LOCUS       DAOSRM010000001        74656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_373_length_74656_cov_100.840940, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000001 DAOSRM010000000
VERSION     DAOSRM010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 74656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 74656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74656
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_373_length_74656_cov_100.840940"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..493
                     /locus_tag="PLA58_00005"
     tRNA            417..493
                     /locus_tag="PLA58_00005"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:451..453,aa:Pro,seq:ggg)
     gene            651..735
                     /locus_tag="PLA58_00010"
     tRNA            651..735
                     /locus_tag="PLA58_00010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:685..687,aa:Leu,seq:tag)
     gene            783..2108
                     /gene="tig"
                     /locus_tag="PLA58_00015"
     CDS             783..2108
                     /gene="tig"
                     /locus_tag="PLA58_00015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOR61208.1"
                     /translation="MSELSVKVEDLSQVKKKMSFEIPWAFVKDELDAVYRTIGKKAKI
                     KGFRPGKIPRKVLETYFKSDAEGEAITNIVNRYYWQELDQRKMVSLSRPEIEQNGLKE
                     DTDFSFSASFETEPEIDPQGYQGIELTRTEIKVSDADMEKRLNEIRRMFASLQDVAED
                     RPAAMGDFVTIDFAGTLDGEALPELKADDYLLELGSKRFVPGFEEQVAGMKKGEKKDI
                     SVTFPADYSESKMAGKNVVFSITLKELKEQKLPELNEEFVKNFDKYNTLDDLKSEVRK
                     SMEEQCKRQSDAKLKDSITEVLLAANAFEAPASLVQRQIFYMIADTQKRMRSAGMDEK
                     SAMELSFRMHDQFKTEAEKTVRAFLLFKKIAEKEAVAVSDEDMDNHIKELSEIHHVGI
                     DSVKSIYEDEEKKESLKAEILQKKVFDFIEQRANIKVVEKIGMGEEAVA"
     gene            2105..2707
                     /gene="clpP"
                     /locus_tag="PLA58_00020"
     CDS             2105..2707
                     /gene="clpP"
                     /locus_tag="PLA58_00020"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOR61209.1"
                     /translation="MNLIPMVVEQDGRGERAFDIYSRLLKDRIIFLGTAIDDNVANVV
                     VAQMLYLEAEDPDKDIFFYLNSPGGHVSSGMAIYDTMQYIKPHISTVCMGQAASMAAL
                     LLAAGGKGKRFALPHSRILIHQPLGGFQGQATDIDIQAREILRLKEDLNKILAELTGQ
                     PLEKVMNDTERDYFMTSYQAKDYGLIDEIVVRKSAASGGK"
     gene            2720..3979
                     /gene="clpX"
                     /locus_tag="PLA58_00025"
     CDS             2720..3979
                     /gene="clpX"
                     /locus_tag="PLA58_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOR61210.1"
                     /translation="MIKRNKDNRGQGMLFCSFCGKTQSEVRKLVAGPTAYICDECIEL
                     CRCIVEEDEKTVEKGHKNVLPEPKEIKRFLDSYVIGQEKTKKILSVAVYNHYKRLFSS
                     AGSNDGVEIQKSNVLLIGPTGSGKTLLAKTLAKFLNVPFTIADATTLTEAGYVGEDVE
                     NIILSLLQNADYDVARASKGIVYIDEIDKIAKKSGNPSITRDVSGEGVQQALLKIMEG
                     TLANIPPKGGRKHPQQEFIQVDTTNILFICGGAFNDLETIISRRIGTNTMGFGAEIKS
                     KKEKRIGDVLAEVRSEDLLKFGLIPEFIGRLPVIAALDDLEEGALVRILMEPKDAIIK
                     QYKKLFELENVNLKFTDGALRCVAKLSMERKSGARGLRSILENTMLEVMYDIPSQRDI
                     KECVISEEVVLNKEKPILIYDQKSESA"
     gene            4004..6412
                     /gene="lon"
                     /locus_tag="PLA58_00030"
     CDS             4004..6412
                     /gene="lon"
                     /locus_tag="PLA58_00030"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOR61211.1"
                     /translation="MTEEKDDTLKNRTNIIPLLPLRDVVVFPHMIVPLFVGREKSIAA
                     LESAMKYEKGIFMVAQKNAKKDDPSEEDIYSVGTIGIIIQLLRLPDGTVKVLVEGKAR
                     GAILEYVRNDDFFLVRVADIDDVDDDPTDVRKQALMRSIKESFELYLKLSKKVHVEMM
                     GTIAGIEDPSKLADVVVTHLNVKLEDKQRIMEIFSIGDRLEAIYSLMLSEIEILQVEE
                     KIKRRVKKQMEKTQKDYYLNEQMRAIQKEMGEKDDFKNEIAELEKRLKQKKLSEEATK
                     KVRQEIKKLQMMAPMSAEATVVRNYIDWLLDMPWSEVTENKHTLKESEAILDEDHYGL
                     KKVKERIIEYLAVQSLVKKNKGPILCLVGPPGVGKTSIAKSVARATNRKFVRISLGGV
                     RDEAEIRGHRRTYIGAMPGKIIQSLKKAGSNNPVFCLDEVDKLSSDFRGDPSSALLEV
                     LDPEQNVAFNDNYLDVDYDLSDILFITTANVLQTIPAPLQDRMEVIRIAGYTEQEKMQ
                     IAIKFLLAKEMEANGLTAESIEFTNQALLRIIRQYTREAGVRNLEREIASICRKVAKE
                     IVSNGNCKKIVISSKNIPKYLGVPKFRHGETEENNQIGLTTGLAWTEVGGELLAIEAS
                     IMEGTGRMVMTGKLGDVMQESVQAALSYIRTRAERFGLPKNFYKKIDIHVHVPEGAIP
                     KDGPSAGIAMATSIASALVKKKVRADLAMTGEITLRGRVLPIGGLKEKILAAHRGNIK
                     TVLIPKDNEKDLTEVPRNVQNAMKIVFVEHIDEVLEIALVKEDDLFGNVVGNEAQADS
                     CAVN"
     gene            6490..6565
                     /locus_tag="PLA58_00035"
     tRNA            6490..6565
                     /locus_tag="PLA58_00035"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6523..6525,aa:Val,seq:tac)
     gene            6601..6677
                     /locus_tag="PLA58_00040"
     tRNA            6601..6677
                     /locus_tag="PLA58_00040"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6635..6637,aa:Asp,seq:gtc)
     gene            6843..7658
                     /locus_tag="PLA58_00045"
     CDS             6843..7658
                     /locus_tag="PLA58_00045"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOR61212.1"
                     /translation="MMKKISDLLSWSSPEQIRRELFSVLAKAQKGGVLDDASRKMIEN
                     ILNFTSTLVREIMIPRTDIVSISAEDSAQEMMREMVEAHYTRIPVHRGSVDNIIGILN
                     IKDLLAAWSPRMSAADILSHMTRPYYIPETKNAHLLFYELKNNNKHIAIVIDEYGGTS
                     GLITLEDLLEEIVGDIRDEHEEQADADDIVRMADGSIVTDGRTEIGKIEELLEIRLDR
                     GRFETVGGLILYAIRRIPHQGESFQIEGMDITIENADERSIKKVKIKKSEDAP"
     gene            7655..9226
                     /gene="lnt"
                     /locus_tag="PLA58_00050"
     CDS             7655..9226
                     /gene="lnt"
                     /locus_tag="PLA58_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOR61213.1"
                     /translation="MKTNGNNIKPDWTGGTPQTRDFFFALLCGILFFLSFPRFGFGVI
                     AWIALVPLFVALNNVSSLSRALLLGWTAGMAAYIGMLYWIAHVVVNYGYLPLVLGVIL
                     TILLAGYLSVYFALFAAGIVYFRGRVPLYLAAPVLWICLEHLKSGLFTGFPWENLGYS
                     QFSNLLFIQMADITGVPGLSFLIVLLNVAFFTLLRERSKKSLALALAVFLIWSGVYAY
                     GAMRIREIRQSMGNARSMDVSLIQGNIEQAVKWNQDFQTETIRIYEELSLRGAKAPGT
                     LIVWPETAVPFNFQEPGVLRARVRQLPSQTKSWLLFGSVSYAAAPAGTDYFNSAFLLS
                     PGGDLAGKYDKVHLVPYGEYVPLRTLFPFVSSLASGIGDFAAGKGYFPLRMDEKKIGV
                     LICYEGILPQAARRYKNAAADVLVNITNDAWFGRTSAPYQHLSMSVFRAVETRLCLAR
                     AANTGISALVDPTGRILAQTKLFERDTITGRIPLMQTPTLYARYGDWFVWAGFIGLAG
                     LFLWSLTRRKRNVRR"
     gene            9213..10323
                     /gene="prfB"
                     /locus_tag="PLA58_00055"
     CDS             join(9213..9284,9286..10323)
                     /gene="prfB"
                     /locus_tag="PLA58_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOR61214.1"
                     /translation="MSVDNLQESILDLKTRIASIGECLDVGELELRVKDLDILSAKKD
                     FWDDPEKARSLLQKKTKLSESLERWKRFSGRIRETENLWNMASEENDQGLLLDLEADL
                     ARLSAEVREEELKMMLASEQDPMNAIMSIHAGAGGTEAQDWVEMLLRMYLRWAEKRNF
                     KTAIIDYLPGDEAGVKSVSFTVEGEYAYGYAKAEIGIHRLVRISPFDAGARRHTSFAS
                     VFVYPEIDDEIKIDIRDDDLRIDTFRSGGKGGQHVNKTDSAVRITHLPTGIVVQCQNE
                     RSQHKNKSMAMKYLKSRLYERELQQKNEKLDEENKQKKDIAWGSQIRSYVLHPYKLVK
                     DHRTNLEIGNAQKVLDGEIDDFIQAYLMAVSNRKS"
     gene            10511..12178
                     /locus_tag="PLA58_00060"
     CDS             10511..12178
                     /locus_tag="PLA58_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOR61215.1"
                     /translation="MRKYKKYNLSRGLLIFLFMIVGFGFAPADRAEAQLPEVSVLFQN
                     PQSVSSPESQAGPPANPAVREDPNAAEEDSADLEEEQDLMENVLAILEVADNYWKNGD
                     IENTLNELDKAYALLLDTNGDVEIARQKDDLRLLISQRIIAVYSSKKGPTNGKASEIQ
                     LIMNPDVEKEIRSFQGPEREFFTSSYLRSGLYRELIVSELKKAGVPEELFWLPLVESG
                     FKVSAYSPARALGLWQFIPSTGYKFGLTRDEWVDERMDVLKSTRAAIAYLKELHAMFG
                     DWLTVLAAYNCGEGRVLRVISGQHINYFDRFWDLYSRLPHETARYVPRFLATLHIVKN
                     PAKYGFELQTTTDTLMNYETVKVGKMMKLSDIASKMEVSEDYMNVLNAELRYKITPDR
                     EYDFKVPEGSLQKFQLVYNDIPAAEKPRLGYAERNKNTGPGRYIRHRVRPGETVDSIA
                     KRYRVSRQSIYSCNRINRNKRLSQQAKYVRIPIDSAETASAKQKTTGGKNASSAGSAK
                     TYKVRKGDTLFSIATRFNVPVAKLKQANRLTTNEIKYGTTLKIPAQG"
     gene            complement(12181..12639)
                     /gene="ybeY"
                     /locus_tag="PLA58_00065"
     CDS             complement(12181..12639)
                     /gene="ybeY"
                     /locus_tag="PLA58_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOR61216.1"
                     /translation="MKIRIANRQKRTKIDSRRIRGNVIRLLRLIGCAEKEISISFVDD
                     EAIRLLNRQYLRLDKPTNVLSFSLEEGECGKINPGLLGDVVISVDTALRDAKQGGFTL
                     EEEILFLIIHGLLHLTGYEHVGTSRANALKMKKREKELFLALTASDAPFA"
     gene            complement(12593..14923)
                     /locus_tag="PLA58_00070"
     CDS             complement(12593..14923)
                     /locus_tag="PLA58_00070"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOR61217.1"
                     /translation="MNNFLDSTSEKILSFFRKRKEKHRFSFAVLNDVIVQRWVIAVLL
                     SAGLTALLAPQFYSSKPVYRHGMIVQENVKADRDFLVEDVQSTRLKQLESAAAVRPVY
                     DYDPETAMTIRAKIKQSFALAAGQRETSQVKALLDRSLGVTLSQEEFAYLSAHAFSDE
                     LLQRFSRIIFAFYENTFVTTGPFGNQEREKGITIVNTLTRTEENTKELSSILLMKDIG
                     PALSKKINASMRNEQPEARRVTFLFLKKMIAPNLTYNPKATAQKIASARESVKPVYFQ
                     VQKNEMIVREGDKIGYPELAKLEAFHKTAAVSNISSLAVLAGIFCIVLFLTLLLYFWR
                     TRNWLKTSPRSNVDFLVFAIVALLQILFVKAGIFISVAVNRAFPSLPVDACYFAIPFA
                     MGAMIIAVLVNRNVAMIMSVLSSFLISLLFEEKILFPLFSFLGSVAASYHVVNSRQRS
                     TFLKVGVFLGVVNIAAILSLNLLTGRLLDDLILRLAMGLFGGIITGILVAGLTPVFES
                     LFGFITYIKLLELANLNQPLFQKMIIEAPGTYHHSIIVASLVESAAEAIGANALLAKV
                     SAYYHDIGKLAKPHYYIENQTGYDNRHDKLSPKMSALIIISHVKEGCELAMKAKLGQP
                     IINIIREHHGTSLISYFYDKAKKNKDESIRSLTESDFRYPGPKPQTKEAGLVMLGDVI
                     EASSRTLTNPTPARIRSLVRERIARIYTDGQLDECELTLRNLNTIAETFTRILTGIFH
                     HRVEYPEAPPKPKETNGKREVYENSNRKPAEADENR"
     gene            complement(14920..15777)
                     /locus_tag="PLA58_00075"
     CDS             complement(14920..15777)
                     /locus_tag="PLA58_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOR61218.1"
                     /translation="MSVTGEDTAVAIASGIIRQLYEMMQKGWRVHLSDFDYAHRILSE
                     DIHFDLASIFLETVFISSQKRVISPKSIAQRHYIDAMRNADMALAIGPAGTGKTYLAM
                     AMAVSCLLEKKVQRIILTRPAVEAGERLGFLPGDLAEKVNPYLRPLYDALYDMVDMEK
                     AGALIAKGLIEVAPLAFMRGRTLNDSFIILDEAQNTTSEQMKMFLTRLGFHSKAVVTG
                     DITQIDLPMERSSGLVEAAAVLRKIQAVRIVHFSQVDVVRHPLVADVISAYAAWENEK
                     KNGKNEARP"
     gene            complement(16052..17293)
                     /locus_tag="PLA58_00080"
     CDS             complement(16052..17293)
                     /locus_tag="PLA58_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOR61219.1"
                     /translation="MIDEVLFEKVSRRIDQSRQDMIELQKALTAVPAVGPANGGDGEM
                     PKALALKKRLVKMGFTIFRHFDAPDPTVSGGLRPNFLTSLPGKDDSRRIWIITHLDIV
                     PPGEPGLWSADPYRVYVKDDCVYGRGTEDNQQDMVASVFAARALLEEGIPPPATVNLL
                     FVSDEETSSEKGVQHLLGLPEKIFSPGDWIVAPDSGNSEGSLIEVAEKGILWLGFRTT
                     GKQCHGSKPQLGINAFSAASRLVIELTELRKIFDAADPLFDPPVSTFEPTKKEANVGN
                     INTIPGEDVFYMDCRILPCYRIDDVLSEIKKITKSIEAAYRVTIEISIHQRVDSAPST
                     PADAPVVRALTDAVNRVYQIRAFAGGVGAGTVAAYFRKQGLSVAVWSKTNMKAHQPDE
                     NCPIGNMTGDAKVFAHLFLQA"
     gene            complement(17308..18738)
                     /locus_tag="PLA58_00085"
     CDS             complement(17308..18738)
                     /locus_tag="PLA58_00085"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOR61220.1"
                     /translation="MKNRKAFFPAAGLLLLAIFAMSPPSAPAESPWISPDQIAYPALE
                     FRMPQAQRIALTNGMVLFYLKDGELPLVSLTVLVRTGSLYDPEGKEGLAELTAALLRT
                     GGTTKISSGEMDRRLDFLAASPSFSMSLDSASVHFSFLKSDLEECLGLLSRMLLEPAF
                     EEKKIQEALALKKEELRRIADDPQKLAFREFNRLLYPGDPRGRYATSASIQNISRADL
                     AAFHREYFSPANMLLAVSGDISREEAVEKIEQAFSDATIPRRVHELPDAPKQRGRGIF
                     LIPKAIPQSTVVSGELTISKNHPDFYAFEVLDFLIGSGGFSSRIFSAVRNEEGLAYSA
                     GSFYRARSNYGVFGTYAFTKTASTFRAISLIDAILKDAAAGSFSQTELDRAKRSIINS
                     FIFSFEQPGQIASQQMTVEYEKLPADFLTAYRTKIEAVTLEDVRRVAAAFLHEKERLT
                     LIVGDADRFGAWPQNWAPPSQFVPQH"
     gene            complement(18728..20254)
                     /locus_tag="PLA58_00090"
     CDS             complement(18728..20254)
                     /locus_tag="PLA58_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOR61221.1"
                     /translation="MRTKNKIWIALGLLLLACIALPAQAVDLEKKIVKARLQNGLTVL
                     MMERKFSPTVSLYIRYRVGAVDESPGQSGAAHFLEHMMFKGTTTIGAKNYPAEKKLLS
                     DMEKIGRELDRERAKQGSADARKIRRLEARMKRLQDKHLRYYIPNEIDRLYTENGASG
                     MNASTGQDMTTYFINLPANKIELWARIESDRLLHPVFRQFYTERDVILEERRQRVEAS
                     PDGKLYEAFMERAYSTHPYGIPIVGLPKDLTFMSPAAIHAIHRKYLSPRRIVIAVVGD
                     IDPSKTMELIRRYFGKIPDAGAPAARIPAEPEQKAERKAQVWFDASPSMIIGYHKPAP
                     PAREDYVFDVLETILSKGRTSRLYDRLVISRQIAQSISAANGVPAARYPNLFTIFARP
                     RHPHTNEELQTAILGEIEAIKTAPVTEEELARAKNQLKMDFIKSLDSNSELASILSYT
                     ELLLGDYRYFSDYLPQIQKVTAGDIQAAAIRYLTAENRTVAVLNRKTAPEEGGGAHEK
                     "
     gene            complement(20238..20477)
                     /locus_tag="PLA58_00095"
     CDS             complement(20238..20477)
                     /locus_tag="PLA58_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61222.1"
                     /translation="MELLVRIKNIVVFLLSGFFLVFGVLLLMSSFQLANPLEFVMTLF
                     AASFIILFCIAGILYAYFRFFQGNSISEEDHADKE"
     gene            complement(20455..21843)
                     /locus_tag="PLA58_00100"
     CDS             complement(20455..21843)
                     /locus_tag="PLA58_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR61223.1"
                     /translation="MSKNILLINPWIYDFAAYDFWMKPLGLLTLGSLLRANRHRVSYI
                     DCLNPYSEPMLKKTGRSPKRHAYGHGRFYRQVIEKPSCLKAFPRSYCRYGMDPDVFRA
                     ELGRFSDTDIVLVTSMMTYWYPGVFDAIQIVRETLPGVPVVLGGKYASLCHEHALAES
                     GADYVISGRGEKQILELLRGIFNEEILFSPEENDLDALPYPAFDLADRIDQVPLITSL
                     GCPYRCSYCSSPLFYKQHLRRDPGKIADEIEYWHKNFGVADFSFYDDALLVNPESSIV
                     PLLHDIKRRGIDCRFHCPNGLHVREIDAPLSRLLFASGFKTIRFGFETARFEAQEKTG
                     GKVRNDELRRAVAHLKEAGYKTRDIGVYLLCGLPDQKAGDVSGSIDFVLRCGARPVLA
                     EYSPIPGTKMWPEAVAASVFDIQREPLYHNNTLLCCRNNDFSYEAYRRMKERVKALPW
                     ETGLPDGTTGSN"
     gene            21989..22438
                     /gene="dtd"
                     /locus_tag="PLA58_00105"
     CDS             21989..22438
                     /gene="dtd"
                     /locus_tag="PLA58_00105"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOR61224.1"
                     /translation="MRAVLQRVDRACVSINSQLFSATDQGLLVFLGVEKGDTEKDADF
                     ILEKTVHLRIFEDEQGKMNRSLVDIAGEMLVVSQFTLLADCDKGRRPSFARAQEPEKA
                     RALYEYFVRRAQPRVKKLATGAFQEMMQIELVNSGPVTILLDSRKMA"
     gene            22441..22905
                     /locus_tag="PLA58_00110"
     CDS             22441..22905
                     /locus_tag="PLA58_00110"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HOR61225.1"
                     /translation="MDESDIKIDKEGIWYYRGAHMFRKDILCVFFEHLKQDDCGRYFV
                     ELNREICYLDVEDTAFVVTAVYRNQTPCGAEELEILLNDDQKEKLAADTLAVGKDNVL
                     YCRVKNNRFPARFTRKSYYQLAEFIETDGADNKFCVVLNNRKYPIAHTSLNP"
     gene            22920..24380
                     /gene="guaB"
                     /locus_tag="PLA58_00115"
     CDS             22920..24380
                     /gene="guaB"
                     /locus_tag="PLA58_00115"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOR61226.1"
                     /translation="MLDDVVKESLTFDDLLLVPAASSVLPRDVETATWLTQKIRLNIP
                     IVSAAMDTVTESRTAICMAQEGGIGIIHRNMSIERQAIEVDRVKKSESGMVVDPITIE
                     PERKVSDALALMHQYSISGVPVVKNEKLVGILTNRDLRFEENLEQPVSQVMTKKNLIT
                     VKSSITLEESKKLLHKHRIEKLLVVDDENRLKGLITIKDIEKIRRYPNACKDSLGRLR
                     VGAAVGIIDREPRIEALLAAGVDVIAIDTSHGHSAGVIDAIRSTKANFPKCELIAGNI
                     ATAEGARALIEAGVDAVKVGVGPGSICTTRIVAGVGVAQMSAIRDTYKVASQKGIPVV
                     ADGGIKYSGDIVKALGCGANAVMIGGLFAGTEESPGETILFQGRSYKVYRGMGSLEAM
                     KMGSRDRYYQDDIEAPSKTVPEGIEGRVPFRGSLSASIVQLIGGLKAGMGYVGCRTIA
                     ELIEKSRFVRITSAGLRESHVHDVIITKEAPNYWID"
     gene            24387..27857
                     /gene="dnaE"
                     /locus_tag="PLA58_00120"
     CDS             24387..27857
                     /gene="dnaE"
                     /locus_tag="PLA58_00120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOR61227.1"
                     /translation="MKQADFVHLHVHTQYSLLDGMIRLDELFKKAKEFAMPAIAITDH
                     GTMFGAIDFYKQALANNIKPIIGCELYVAPRSRLDKTPSAVGDTARHLIVWVKNLQGY
                     KNLLKLTSVAHLEGFYYRPRVDKALLAECSEGLIASSACLHGEIASHIVRGHMEEARR
                     AARELQKIFGEDNFYLELMENGIPEQKIANQGLMELSRDLSIPLVATNDCHYLEADHA
                     EAHDVLLCIQMGKTIEDKDRMSMSSNQFYVRSPQEMQTLFAATPEAVANTLAIAESCN
                     LTFEFGKFYLPKFEVKNPEETLEDYLDRKATEGLEKLFPVILKYAKEDEAKVREKYTR
                     RLRSELAIIKKMGFAGYFLIVSDFIKHAKHTGVPVGPGRGSAAGSLVAYAIRITDIDP
                     LRYGLFFERFLNPDRISMPDIDIDFCQERRGEIIKYVTEKYGKDKVSQICTFGKMMAK
                     GVIRDVGRALNIPYGDADRIAKLVPSFPLNITLAEAFKAEPRFAEERRKNPKIDKMLS
                     LSLILEGLNRHSSVHAAGVVISDAPLVERVPLFAPKDDIVSQYSMKDIETVGLTKFDF
                     LGLKTLTVIKNAVDFIRASHNVDLDVNNLPLDDEETYRLLSRGETDGVFQLESAGMKD
                     LLVNFKPDRIEDIITLIAAYRPGPMQMIPDFIARKHGKQQITYELPQLEPILKETYGI
                     ILYQEQVMQIANAIGGYTMSQADTLRKVMGKKQIAAMEKEKPRFLEGAKKQNINENKA
                     KYIWEQMEKFAAYGFNKSHSAAYAMITYQTAYLKAHYPVAFLAALLTSEKDNRDKIIK
                     HMSNCKEMGIDILPPDINESQKDFSISGGNIRFGLTAVKNVGEAAIESVMDIRKEGKF
                     TSFMDFLCRIDLRKVNRRVLESLIKCGSFDSLGYTRRQLMECLDAAMEVAQRKQKEIL
                     SNQASIFDQLGAEERAETKGSGSFSIPDLPEWDRKELLAIEKETVGFYISGHPLHGYA
                     GKLNLVANTDSSTLNNKRDKETVTIAGIVSSLSERLTRKKDVMCNVVLEDLQGSASII
                     FWADIYRKYSELLHADEPVVIQGIVDVGDESNKIIAQEVIPLARALENPYKQVRFIVR
                     TDKIPPEGISALNDTIRKYSGKYEGYIHLLNGKCETIVRLGDRTKLDICEKLRREADA
                     ILGEGSTVYC"
     gene            27901..28638
                     /locus_tag="PLA58_00125"
     CDS             27901..28638
                     /locus_tag="PLA58_00125"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOR61228.1"
                     /translation="MTSNANMIYEERTYRSLINKDNLISYQVKVAQSDLWISSDTDLS
                     GLAEKSLRQHRYSLESYIHRYPEFRTTLLPFPQDPLAPAIIREMIEQSTVCGVGPMAS
                     VAGAIAEFVGNDLAGLTDNIIVENGGDIFLRSKKSLSIGVFAGESTLSYKVKLIIKPD
                     ETPLGICTSSATVGPSLSFGKADAVCVLSRCATLADAAASAVGNCVKSKNDIRKALDF
                     GLKIPGVRGVVIIIENEMGAAGEVTFF"
     gene            28690..29082
                     /locus_tag="PLA58_00130"
     CDS             28690..29082
                     /locus_tag="PLA58_00130"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOR61229.1"
                     /translation="MNEFTEEIWKRSPFIKLLGLEIEKIEHGACFSRLRIRDTLLNKH
                     SVVHGGVIYSMADISMGVAVYSTLKSGEETSTVEIKINYLKPAKTSLLTCEAKILKKG
                     KNIAVMEADIRSGENSLIAKALGTFMFT"
     gene            complement(29189..30151)
                     /locus_tag="PLA58_00135"
     CDS             complement(29189..30151)
                     /locus_tag="PLA58_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOR61230.1"
                     /translation="MAEDYYQVLGVDKKATADEIKKAYRKLAVKWHPDKNPNNKAAAE
                     EKFKKISEAYAVLSDAEKRQNYDNFGSADQFRQQYSQEDIFRGFDLDEILRSFGFGGS
                     RGGGRTTFRTGTRRGGYTEYDDPFDGMFGQMGGGAAGRRYADMPQKGQDVEYNLSISL
                     EESVMGADKKISLQMENRIEDINVKIPPGISTGKKLRLPGKGLTGYQGGPNGDLYLNI
                     NVLPHPIFARDGNDLYIEKTIKFSQAALGTTIDVPTLDGSTKRLKIAPGTQNNTKIRM
                     KGFGVPGMKGAPKGDQFVKINVEVPKKLTDKQIRMIEQLAADGI"
     gene            complement(30200..30781)
                     /locus_tag="PLA58_00140"
     CDS             complement(30200..30781)
                     /locus_tag="PLA58_00140"
                     /inference="COORDINATES: protein motif:HMM:NF005322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOR61231.1"
                     /translation="MKDDKVKSILFAGVGGQGILRASDILCEVMMEAGYDVKKSEVHG
                     MAQRGGCVTSHVRYGAKVYSPLAEPGTIETLVSFEKMEALRYLKYLSADASIILNTEQ
                     INPPAVNLGDSQYPDDIIEFLKNNYPRVIAVDALSLALKAGNLKTANVVLLGTLSSLM
                     DIKTSIWESVIRKSFPQKLVKLNLEAFQMGIPA"
     gene            complement(30798..32564)
                     /gene="iorA"
                     /locus_tag="PLA58_00145"
     CDS             complement(30798..32564)
                     /gene="iorA"
                     /locus_tag="PLA58_00145"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOR61232.1"
                     /translation="MKKVLMSGNEAIARGVYEAGVRFAAAYPGTPSTEIMEQFARYDG
                     VYAEWAPNEKVAVEVAIGAALGGARALAVMKHVGVNVAADPIFTVSYTGIAGALVIIS
                     ADDPSMHSSQNEQDNRNYAKFAKIPMLEPADPQEAKEYVKLAFALSEQFDTPVFLRTT
                     TRVSHSKSVVTLGEPRIPEDKTTLAHHAAKYVMVPINARTRRVEVEKRQKALLSFAET
                     FPENKMEINDPEVGIITAGMPYNYAKDVFPNYSYLKLGMVYPLPVKMIRHFASRVKKI
                     YVVEELDPYLEEQIRAMGIEVTGKEIFPYTNEFDPGVIRNSIASGGQDAVSPYPGSLA
                     QRPPNLCPGCPHRGLFYALKKSKTYVHGDIGCYTLSYLKPLEGLHSCICMGASIGMAH
                     GMSKALKDKGKGKVVGVIGDSTFLHSGIAPLMNMAYNQSDALIIILDNSTTAMTGMQE
                     HPGTGYTLMGKETKKVNLNILISALGIENIRVIDPNNVKEARIAIKDELASSGPSVII
                     AQSPCVLFKRANQKPKPPLKVDPDACEGCRACLELNCPPIAWKKGVTKEGSKRKGIAE
                     IDETLCNGCGLCAQVCKFGAIG"
     gene            complement(32614..33294)
                     /locus_tag="PLA58_00150"
     CDS             complement(32614..33294)
                     /locus_tag="PLA58_00150"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR61233.1"
                     /translation="MAAKLSKKELKSPDAFQTAFEKVGEYVEENKSRVLIAGTALACA
                     VLLVLGIYFYWSYYSGAALKLYAQAQENILKSGDNPQTADANIPVFQKLTDQYSYTWS
                     GRMAHYHLANIYYNKGDMDRAITSYEKFIAKTGSDKTGVKFLALTSLGYAYEAKKDFR
                     AALKYFEQAQKVYHTGFEMMSLRNLARACEELNDKAKALEYYKKALEKTTEPAARIFI
                     QRKIAALG"
     gene            complement(33296..33715)
                     /locus_tag="PLA58_00155"
     CDS             complement(33296..33715)
                     /locus_tag="PLA58_00155"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOR61234.1"
                     /translation="MESNLTKIRVIYADTDAMGIVYHANYIRWFEHGRNELMRQVGIV
                     YSDLNQLGLHLPLTQVFCRYLLPATYDDLLTVETSFDYIKRASIRFGSKIWDENRQKL
                     HAEGYTIHACTNSVGKIRRMPALITGLIEKYSISKGE"
     gene            complement(33705..34931)
                     /locus_tag="PLA58_00160"
     CDS             complement(33705..34931)
                     /locus_tag="PLA58_00160"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOR61235.1"
                     /translation="MKYVVLLGDGMADYPSRQLGGKTPLQCAVTPFLDQIASEGTLGL
                     VDTIPEGFTPGSDVANLSVLGYDPKETYTGRGPLEAASQGIALGQGDIAYRCNLVHIG
                     EPQEGTAFMDDFTAGHISSPEAREIILDLNKKLNTSRYQFYPGVGYRHLLVWRNAAGS
                     PQTTPPHDIPGKPIVPFLPQGDCAEEILGLMRAAADILKDHPVNAARVADGKKPANSI
                     WLWGQGGKPQMAPLTEKYGLRGGMISAVDLLNGLGIYAGLKPLRVEGATGYIDTNYEG
                     KAAMALDALKFMDFVFLHLEAPDEMGHEGNAKGKILAIEFFDEKIVGPILTRIGERGE
                     YRILVLSDHPTPIDVKTHVGDPSPFAVLSSRREENLARGLKFSEAQAGAAGLVVSPGH
                     LLMDKFLRDWSAFLGK"
     gene            complement(34990..36297)
                     /locus_tag="PLA58_00165"
     CDS             complement(34990..36297)
                     /locus_tag="PLA58_00165"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOR61236.1"
                     /translation="MKNISIGLIGLGNIGTGVVRLLEQNEKLIAQKLGAKLVLKKIAD
                     IDITSPRGVKIPKKLLTTDARDILNDPDISIVIELMGGYEPARTFVLEAIKKGKHVVT
                     ANKAMLATFGNEIFPAAQKKNVDIGFEASVGGTIPIIKTLKESLVANRIHSIVGIMNG
                     TCNFILTKMTNEGKPFDVVLKEAQQLGFAEADPTFDIEGIDTSHKMAIVLSLAYGKKV
                     RLKDIYLEGITKISAEDIAFAGELGYRIKLLAIGRLKGDAVEARIHPTMIASGHLLAN
                     VNHNYNAFHLVGDASGPVFLFGQGAGMMPTASAVFSDILDSARNVLKGISGRVPLRSI
                     DETGMKDIRLVPMDNIETKYYFRFTALDRPGVLSKISGILGAHDISLATVIQKAREEA
                     GAVPIVMTTYKAKEKNVRQALKKIDRLDIVRDKTILIRIEDEL"
     gene            complement(36436..37353)
                     /locus_tag="PLA58_00170"
     CDS             complement(36436..37353)
                     /locus_tag="PLA58_00170"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOR61237.1"
                     /translation="MRIAVVGIGGVGGYFGGKLAREYASSGKHEIIFIARGEHLRAIQ
                     KNGLQLYTREGQYTAWPNIATDDPSLAGPLDLVLLCVKSYSLEESARSLAPNISRKTT
                     VIPLQNGVDSKERLRAVLPGADIASGCVYIISFLDQPGVVIQTQGACRLIFGTDNRAS
                     ADRYKNILDLLLAAKIDARLTDKITEALWMKFLMMCPLGSLTAATGKTYGFIREDESL
                     RERLRAMMMEVAAVAKARRIRLPQNAVDKTMEMIGGFSHDAKTSMQLDREKGKQTEVD
                     TLTSFLCRAGRDAGIPTPLHDEVCRQLTP"
     gene            complement(37424..38092)
                     /locus_tag="PLA58_00175"
     CDS             complement(37424..38092)
                     /locus_tag="PLA58_00175"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOR61238.1"
                     /translation="MHLKNSFLRHFLLVLSGLILACGCPPCLYADHDTALRTDLNFSY
                     TFNEKFRGISYAYIQADDEMSNYDYAEWGIGLNYQLPLPWLSFQIQYCQGYTKDEGRS
                     WALEQDPGASLNLRTKIGPFSIEEQILFEYRILPEWNDFRLKNILVISLPAALLTPYA
                     GLESYYENREKALMLNRLKIGINAAVSRHISAGPYYRMDFSNVDHSWEWARQLIGVQL
                     SINY"
     gene            complement(38322..38627)
                     /locus_tag="PLA58_00180"
     CDS             complement(38322..38627)
                     /locus_tag="PLA58_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61239.1"
                     /translation="MKMKRMIAVLTVAAFLAVSMAAFAAGPGKKPDRVPPKLQSAILG
                     NEMLWDRPEAFGPVPKSKRNLGKQVCGAEKKAVGYHPKAQDENGKAFPKGGFLCAPK"
     gene            complement(38720..41185)
                     /gene="recG"
                     /locus_tag="PLA58_00185"
     CDS             complement(38720..41185)
                     /gene="recG"
                     /locus_tag="PLA58_00185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOR61240.1"
                     /translation="MESFKKNLQRIEQPLAFAAKDHFKNLPNIRNLGKSLSGLIALQI
                     DAIPSAAKNLFGPALDHMMRIFLEYDEKDEDRKRSSIAEALVILERLRHAAENFDARH
                     PAAPLREEQIAARINDLKTSCEKLSAPVQFLKGVGPKMALRFAAKKIFTVEDLLYFLP
                     RTYEDRREIRKIFKLETNRTQTVVAAVSEAAFRYYGKRRILEVAVSDDTGILTAKWFK
                     GRIAHLAGAFKKGTRVIFTGNVTPGYTGKSMIHPDYEILDEEDEENLLHFKRIVPVYS
                     ETEGLHQKYIRKVMASALENYSRYIASPIPADICRKRSLINIREAIVSVHFPGSDAEM
                     APFLDARSEAHRRLIYDEFFFFQLGMAVRKSGRVLEKGISFSRGGNWLAKFFASLPFE
                     LTGAQKRVVEEIQKDLQKPTAMNRLLQGDVGSGKTLVAMSAMITVCENGYQAALMAPT
                     EILAKQHDATLCAWAEPLGLRVVLLTGGMNGAARTEALERIRSGKADIIVGTHALIQE
                     DVDFRKLGLVVIDEQHRFGVLQRATLRNKGLSPDVLVMTATPIPRTLAMTVYGDLDVS
                     VIDEMPPGKKPVRTVVMGENRREAVYEAIRKELANHHQVFIVYPLVEQSENLDLKDAT
                     KMAEHLQQDVFPDIRVGLIHGKMKDREKDAVMTDFLENRIAILVSTTVIEVGIDVPRA
                     SLMVIEHAERFGLSQLHQLRGRVGRRDIPSACILLADYKASGDARKRLKVMEKTTDGF
                     VLADEDLAIRGPGDFLGTRQSGMPDFRIASIIRDARILNDAKEDAFALAARDPLLTKP
                     EHAILKETLIAKWQGKLDLARTG"
     gene            41348..42415
                     /gene="purM"
                     /locus_tag="PLA58_00190"
     CDS             41348..42415
                     /gene="purM"
                     /locus_tag="PLA58_00190"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOR61241.1"
                     /translation="MPNKSVKSKPSTYKESGVDIEKANAFVERIKPLIKTTARKEVLS
                     GIGGFGGLFRFDAAKTKNPILVSSTDGVGTKLKIAQLMDKHDTVGIDLVAMSVNDVVV
                     QGAEPLFFLDYLATGKIEPEKSVAIVEGIVQGCKQAGCALLGGETAEMPGFYPPGEYD
                     LAGFCVGVVESEKLIDGSTISINDRVIGLASSGLHSNGFSLARKVLLEKGRLSVKDTV
                     SGLARTVGEELLEPTRIYVKPLLNIFKSFPVKGLVHVTGGGFYDNIPRVVPDVCRCVI
                     RRGSWEIPPVFSVIRDIGHVEEQEMFRVFNMGIGMIMVVPEKDAQEILDRLKGLGENA
                     CLLGAIEKRDENGPPVCFTDL"
     gene            42425..43084
                     /gene="purN"
                     /locus_tag="PLA58_00195"
     CDS             42425..43084
                     /gene="purN"
                     /locus_tag="PLA58_00195"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOR61242.1"
                     /translation="MADFLKLGVLISGSGSNLQSIIDQIERGALNARIAMVVSNNPEA
                     YGLTRAQKHGIPSAVLNHQDFENREDFDRELLRVLTDAGVELVALAGFMRILSPFFLR
                     AFPDRIINIHPALLPAFPGTHVQQKALDYGVRFSGCTVHFVDEGVDTGPIIIQAVVPV
                     WAGDTAQTLADRILKEEHKIYPQAIRYFADGRIVKNGRRVEIKDSRNHDAVLCNPPLD
                     R"
     gene            43124..44581
                     /locus_tag="PLA58_00200"
     CDS             43124..44581
                     /locus_tag="PLA58_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61243.1"
                     /translation="MYDLLVTGDDLASHVAAAYASRQGLKTLLVAQSGLGGLQIIGDF
                     VFNLDSAPMTGLASGQPGFSVLAELGVEIPETHPTAVSPAYQVLLPGHRIDFSSDPDS
                     LLSELAWEFPDREAELRDFYGSAREASSVFQDWIAEHPRIQPQTLKEYVSYLKIFPFL
                     LQYKFAAARFDRMLSQDAPLEKVWEAQQALLSLNTDDLFSFASAFQYSAPLRGVAYFP
                     QGKQFLFNAMIRTLEEGGGLYLSGHSITSVARHNTITLEVKAPDGSLFQATGRHWIVS
                     TKSDALSLLEANRSHVIFSERLRPARIVGYPFTLFLGVAEKSLPEQMARHIAVVTDVH
                     KDLYDHNVILLETGLPEKEKPLFQAKNFVTATVYLPAEDDVWTGEALRQEAASIMERL
                     DGFFPFLKDGIELYDLDKSIELSLNYRKVLSPKYKVRNAFFTSFAAKSDRTRLKNVFL
                     TGASLLTDAGFDAELLSGKNAAMQVIEERKFSDGT"
     gene            44571..45869
                     /locus_tag="PLA58_00205"
     CDS             44571..45869
                     /locus_tag="PLA58_00205"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOR61244.1"
                     /translation="MEHDFSIACLGPCTIDSPINISGFVSDENRILFNTRLDDYDAMK
                     DAAGKPLSLEPAGPRKKIFFDPAKTKAAIVTCGGLCPGINDVIRSVTMTLFYRYGVKN
                     VLGIKYGLRGLNPEYGKIPIQLTPDYVKDITHIGGSILSSSRGPQDVKVMVDYLELLK
                     IDILFCVGGDGTMRAAEKIATEIHSRKKQIAVIGIPKTIDNDLNLIEKSFGFDTAIAK
                     TVEAVGSAHVEAKGAYNGIGLVKIMGRLSGYITATAALAQGDANFVLIPEVPFELDGE
                     KGFLKTLENRLKASGHAVILVAEGAGQELMKQDGQEKKTDPSGNVRLHDIGLFLKNAI
                     DVYFHRINMEINLKYIEPSYLIRSVPANSSDSLYCSSLGQYSVHAAMAGKTGLLVALR
                     KDEYVHLPLSAAISGRQIDPDGNLWFRVLEATGQPAEMKK"
     assembly_gap    45947..46046
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            46134..46331
                     /gene="rpmB"
                     /locus_tag="PLA58_00210"
     CDS             46134..46331
                     /gene="rpmB"
                     /locus_tag="PLA58_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOR61245.1"
                     /translation="MSRICEVCGKKPAIGNNVSHANNKTRVIWRPNLQKLRCVDEKTG
                     SVKRVKVCTRCIRSGFVKKAS"
     gene            complement(46328..47062)
                     /locus_tag="PLA58_00215"
     CDS             complement(46328..47062)
                     /locus_tag="PLA58_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61246.1"
                     /translation="MHDMDPDIYIYNIAPDLLTIHPDISPRQTHFIPRFIEAPPDHQR
                     TAYVMFHLLVDDLAHYGSISMTCQEGFDPNSSGYTYLKGRNLVGHIMNLHRMIDKEIS
                     FSDAAYQSHLLVEMIYDLVIRQRIDRSGSIDLLEEAVHFTFDRREKEFCSDISWLYGI
                     DPEHSREVLKIAVSYITRERMQRFMNIEGRIRLFTDKFGLRNNNELFTETISALFQDA
                     LLSIGDENFLQEACGTIRNCGWLPTR"
     gene            complement(47242..47628)
                     /locus_tag="PLA58_00220"
     CDS             complement(47242..47628)
                     /locus_tag="PLA58_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR61247.1"
                     /translation="MNVQIIKHKGAPEYAIVPFEEWEKIISRLEELEDIRDARVVRAA
                     IAEGEETYGEDFVKRLCFGKSRLKVWREYRKLTLVDLAKICGVSVAAISQIENGKRTP
                     SVDLLMKLAKALNCDMEDLIQPSKLP"
     gene            complement(47954..49174)
                     /locus_tag="PLA58_00225"
     CDS             complement(47954..49174)
                     /locus_tag="PLA58_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOR61248.1"
                     /translation="MRVNDQNGNSRVEPMEIVEMTVRVQNMGHGDARGVAADVQVGQN
                     VFLAGDAQTHFNLGAIPTGKYRDFKFMFYTNNRIAGGEKIPIVVNLTEARPKFNAQKD
                     PALAMNAPQRRAQEFVVKGEDTAPKQEISLAGGLSVDVDMNIPDGQKAGKFDAAVVIG
                     NRNYSASGLPEVEFAVRDAQVMKEYLVRTMGYDPANILYAEDATVGKFYELFGNERNH
                     KGRLFRLVKEGKSKVFVYYTGHGAPDLETQEAYFVPVDANPQDLKTNGYRLQTFYDNL
                     SKIPAKKITVVLDTCFSGNSDRGMLFKNVSPGILKVKSEYRGPKNAVLITSAAVDQVS
                     SWYPEKRHSLYTYYFFKGLQGEADANRDRKITVGEMDAFLKENVPYMSRRISGNEQNP
                     VMLGSAAEVMAWLK"
     gene            49413..51152
                     /gene="recJ"
                     /locus_tag="PLA58_00230"
     CDS             49413..51152
                     /gene="recJ"
                     /locus_tag="PLA58_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOR61249.1"
                     /translation="MNERGQLPVTQWKIKDSGPKNIEESLVKEFGVHPIVSQLILNRH
                     VDSLEGAQRYLHPSLNDLHSPFLMQDMRKGVARLLKAIYDEEDIVIYGDYDADGITSV
                     VILYKFIKQLTGRVSYYIPDRVQEGYGLKIPVIDQFKQKNVKLIVTVDCGISDIEQIA
                     YAKSAGIDTIVLDHHEIADQLPPAVACINPNRKDCAFPFKRLAGVGIAFNFLIALRGT
                     LRKEGFWKNAEYPNLRELLDVVALGTIGDIAPLLGENRIFARIGLDLMTEGRRPGIKA
                     LKEVSGIDGQLIDSFKASFCLIPRINAAGRIASPLDAVELLLAETLEEARPLAEKLDL
                     HNRRRQAMEKDILNEILEQLGAKPDLENINALVFSSEKWHPGVVGIVASRLVDLFSRP
                     TFVISLKDGVGKGSGRSVSDFNIHKGLQQCAPLLLSYGGHYRAAGISIKEDDIDEFAC
                     MLDEIIRNSVQSSEMVSQTLIDAECRLQDISLDLIRQMSMLAPFGSENPEPVLCSRNI
                     RASSPVIVGNNHLKMRLVDDSASLNAIWFGMGKHLGAINSANLDVVFSPQINYWNGSF
                     DVQLKIRDAAIVA"
     gene            51162..51518
                     /locus_tag="PLA58_00235"
     CDS             51162..51518
                     /locus_tag="PLA58_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61250.1"
                     /translation="MNQETRAALFSFLLFPGGGQIFLKRYARGMAFLAPAAAATLALA
                     WMIFQVVLVIVREALSGASGMDGVLPGMRNGLGAINMNLALWLLILILFLWISSTIDA
                     YRLGKKAEPPTTSANR"
     gene            complement(51496..52749)
                     /locus_tag="PLA58_00240"
     CDS             complement(51496..52749)
                     /locus_tag="PLA58_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR61251.1"
                     /translation="MKRLYDEKEAWGVEERKSRQDRLLARMVRLGYQKSPALRRMLDA
                     NKINPARIKTREDLEILPVTSREKLVEMEMQKPPYAGLENPDVAIERIFTSPGPVYEP
                     HLSETDDLWARAFFAAGIGPKDIALNAFSYHLVAAGLTFHNGLRKVGATVVPSGTSSS
                     QIQVQLIRDLNVTAYVGTPSFLASIIDQSKELGFDFKKDFRVKKACFAAEPLLPSLRQ
                     RFEKEHGIRTFQMYGATEVGDVAYECCAKSGWHICEETIVEIVDPATGKNVAPGELGE
                     VVVTRLNNIFFLLRFGTGDLSRLITKKCSCGRTSFRLAGIAGRVGDAVKVRGLFIAPS
                     QMKLLSAKFDGLPLQAIVSRKGHEDVLTLRVENINSQVNSDGWENNFKKIFKEICTVK
                     IDVLEVVEKGGIKPDQKMIIDLRTW"
     gene            complement(52937..53401)
                     /locus_tag="PLA58_00245"
     CDS             complement(52937..53401)
                     /locus_tag="PLA58_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61252.1"
                     /translation="MDLLNELKKISLKLDQAGIDYALCGGLAIAIYAKPRATLDIDMM
                     ILPDFLSKTKIVLQKIGYTMSSAPMKFNQGAVIIHRLSKADKKSGEYFIVDLLLATPE
                     TRQAWETRLTVDWKGHPLKVVSPQGLILLKSMRKAGQDLDDIEYLRSLEHED"
     gene            complement(53502..54302)
                     /locus_tag="PLA58_00250"
     CDS             complement(53502..54302)
                     /locus_tag="PLA58_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR61253.1"
                     /translation="MGEILLQLNNISVVYSNVIQVLKGVSLSVARGHIVSLLGSNGAG
                     KTTTLKAISGLLKPENGEVTDGDIIFNGVNIQNQAPEFITRQGIIQVLEGRQPFKYLT
                     IEENLRVGTATRPGKPYKEDLEMVYHYFPALVKRKKALAGYCSGGELQMLVIGRALMA
                     HPQLLMLDEPSLGLAPLVVQEIFRIIKKINEEHATTIVLVEQNANMALQVAHYGYVME
                     NGKIVMEGTAQELSDNPDVKEFYLGMAASGIAKSYKDVKSYRRRKRWL"
     gene            complement(54379..55449)
                     /locus_tag="PLA58_00255"
     CDS             complement(54379..55449)
                     /locus_tag="PLA58_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR61254.1"
                     /translation="MKKPSFHPCGNFRESYAEELAVFDTWFGRVSLLVFLILLFGLAP
                     FILDRYLLYILNNIGIMAIAAIGLNILIGYTGQISLGHGAFFGVGAYAGAILATRLNL
                     PFFVCIPAAGLITAMVGMIFGIPSGRLKGLYLTIATLAGQFIIEYVLIQWESLTKGTM
                     GITLPEPNILGVNIAGDKAFFFLIFACLVAMTWFAVNIMRSKFGRAFVAIRDNDRAAE
                     GMGIPIFRYKLLSFAISSFYAGFAGALWAYYMVSITTEPFNLALSVEFIAMVIIGGMG
                     SMPGAIFGATFITVLNEVLRFATGALMNVGWIAGLGLNMAPLREFVFGLAIVLFILLE
                     PKGLAELWRIVRSSFRLWPFSY"
     gene            complement(55451..56329)
                     /locus_tag="PLA58_00260"
     CDS             complement(55451..56329)
                     /locus_tag="PLA58_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR61255.1"
                     /translation="MDFFLQLLVNGVCVGLLYGISAMGFVMIFKASSVLNFAHGELLA
                     MGAFFFLSLATWGKIPVAAAFVLTLAGCFLLGFLIEKFFLRPLIGEKLIFVIMLTVGL
                     AAMFKGLILLVWGGNLHTYPEFLPASLELHLGSITIVPVYSATLVISVAFLVLFGLFF
                     KKSSQGIFMRSVADNQKAALSLGVHVRRVFALSWAIAAMVACMSGIVLGIINGVNVHD
                     LSAIGMKVFPVVILGGLDSIGGAIIGGIIIGLLETFTGGYLSPSLRDVVPYIALVFIL
                     MVKPYGLFGLVEIERV"
     gene            complement(56341..58269)
                     /locus_tag="PLA58_00265"
     CDS             complement(56341..58269)
                     /locus_tag="PLA58_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR61256.1"
                     /translation="MDKSTAHSTKQNRVTLPRLLASNAEKYGDAKTAIREKAFGIWQK
                     YTWNDYFHYVRKTAAGFASLNMTRGDNLCMVVNNSPEWLFSELGAHALGATSLNLFTS
                     SIAEELAFSIGRIRSPLVVVQDQEQADKLLEVKDKLPHVQNVVYVDPTGMTSYENDPW
                     LISYATLLERGEKYLADHPGVVEEEIDKGRPQDIAVMIQTSGTTGVPKIAMLSHRNLA
                     AMAFQWIEAADIQPEENWISMSPTAWIVDQMWGMGVALAGAMTMNFPETPETVLEDFR
                     DIGPSMLITASRFWEDMASRIRVKISDAGWLKRKLFYLAERVGRTVVEKKLRKERISW
                     PVHVLYGFLKLAVYNPLLDRLGCSRFHSAFTGGHPISPDVIGFFRAIGLNLKQCYGLT
                     ESCGIFQIQPDDEVKLETVGRPLPRTSVKLAEDQEVLVRSEANFSGYYNDYQSTEAAF
                     ENGWLKTGDAGYIDDDGHLLIIGRKEDIIRNKSGNAFSPDFIETRLKFSPYIKEAVTF
                     GEARPYITAMINIDLGNVGNWAEERMIPYTTYMDLSQQKAVEELILKEVQEVNRQLPD
                     VMKIKKFVLLYKLLDADDDELTRTGKVRRRFVYGLYIKIIEAMYGGLKSVPVTGKIRY
                     RDGRIGEIETTINIIDLD"
     gene            complement(58285..59172)
                     /locus_tag="PLA58_00270"
     CDS             complement(58285..59172)
                     /locus_tag="PLA58_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR61257.1"
                     /translation="MDPVFPRRKRGVNTRVEVFMKAFRSRPSIMINRPQEDKKAELEI
                     RNLRLSFGGVVAVQDVDMTVHTGELMAVIGPNGAGKTSLLNCITGFYRPQQGQILFNG
                     RDITRLHTHQLVGIGIGRTFQNIELFPGMTVLANMTLARHIHCGYSFLHSCLFTPSVR
                     EEEIRHRRILEEIIDFLEMQSIRKQTVGSLPYGMMKRVELGRALALEPRLLILDEPFA
                     GMNLEEKEDMVRFLLELNGRWGLTMVLVEHDMSIVMSISRRVMVLNFGEKLGEGSPGE
                     ISANPEVIKAYLGDAQSLE"
     gene            complement(59195..60403)
                     /locus_tag="PLA58_00275"
     CDS             complement(59195..60403)
                     /locus_tag="PLA58_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR61258.1"
                     /translation="MKTCGRILLALCILALATTAGQAVAQTKTIKVGAAINLTGPASS
                     WGQFHEKGQRDYFRYVNEVKGGVAGHKIDMITVDTAYKVPEAQAAVQKFVLQDKVDMI
                     ATWGAGEGLAAKPIVQKYKVPTINYSTSWEILEKPIDYMYLPFGSYKMDCRAVLEYIK
                     TIHTGKEAPKVGLLTYNNAYGRSIHAPSREYAAQLGVDLVAIEEFPPRTVDLNTELLR
                     LQKKGARYIFIQMLPSTIITAFKSADRIKYNPLFLGTWTSTDPDFFKMGKGLIRDRLV
                     MQFPGGLPSDKSKGMDVMKELWKRYKTVRSFDASYWEGVVVGMIMERAFLRAYEKSKA
                     INPGTINAALESMKNEDFGGLVPAVSFSKDNHEGSFTARMVKVMENGTYIPLTNFYVP
                     GKDKIKVLKK"
     gene            complement(60678..61967)
                     /locus_tag="PLA58_00280"
     CDS             complement(60678..61967)
                     /locus_tag="PLA58_00280"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOR61259.1"
                     /translation="MEKATLTLNGMKYELPIVEGTENEKGIDISRLRQDTGLITLDVG
                     YQNTGSCISSITYIDGDKGILRYRGIPIEELAEKASFTEVAYLLIYGELPNREQQQLF
                     SQRLTQNSMIHEEMLRFFDGFPPTAHPMAILATMVNSMSVYYTDYFTEDFRAETFDLM
                     AASLISRIRTIAAYSYKHSIGHPHVYPKRDLKYASNFLYMMFDSPVNPYVPDPDIERA
                     LSTLMILHADHEQNCSTSTVRLVGSSMANLYASICSGICALWGPLHGGANQKVIEMLE
                     EIQSSKLSIQTCIDSAKDKTSKFRLFGFGHRVYKNYDPRARILKQYCETIFQKHNYQD
                     PLINIAMELEEAALKDDFFIQRKLYPNVDFYSGILYRMIGIPTNMFTVMFAIGRLPGM
                     IAQWKEMHDTVPLKIGRPRQIYTGSNLRHYIPIDERG"
     gene            62192..63955
                     /locus_tag="PLA58_00285"
     CDS             62192..63955
                     /locus_tag="PLA58_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOR61260.1"
                     /translation="MKKMMMVLLCLVAILLMAATVLSKEKYTVTVLPFSLYSAENIEY
                     VRQGINDMLISRISGSDRIEVTRKDVVSDILKKTGGKELNLADVQGIGQQLKSDYVVW
                     GSITKIGKSISIDGKLIDIAGGKSDVGIFSQSQSLDEVIPKINDFSQRIVRHITGGAA
                     AQTSPVVAAAAPAASAVAGGSPAPPPVPGASREAQIIAGMKSGKGKGTLTSMINAEFI
                     NSSDPLDRKKGFWMSQQFKTEFYGMDIGDVNKDGVNEVVIIDRHNVYIYLKTKNNLQL
                     LKQIKGKSYHKYISVDVADINKDGTPEIIVTSLNDRVLNSFVLQYEDGDYKMIASDIR
                     YFLRVITPSSGTPMLLGQTYGMGSFSDGMGGKVFDTQIYEIVWRDGKYVEGDKQKIPV
                     GLSIYGLTLDSLGAGTSEKIIALDELDYLCIITPTNKPLSRIFSFGFSPDELIWRSDS
                     QYGGSNNYIDNVDKQKQQSTEATQEEGAFANLRILMFDTNNDGKKEMIIVKNLSSVGR
                     VFKNLKLFSSSEIYNLEWDGLGMAENWRTKKINGYVADYCFKDVDNDGKPEIVLALVQ
                     SVGATLSERSVLVVYELEMPQ"
     gene            64023..64099
                     /locus_tag="PLA58_00290"
     tRNA            64023..64099
                     /locus_tag="PLA58_00290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64057..64059,aa:Met,seq:cat)
     gene            64162..65154
                     /locus_tag="PLA58_00295"
     CDS             64162..65154
                     /locus_tag="PLA58_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOR61261.1"
                     /translation="MSAEPGNHHASFQPRSFLKNRHLQSVLASSHLIIPPHGPLIRHS
                     REFIIETPGGSKLLSYLSPHPHSRGMMILLHGWEGSSSSAYILAAGAYFFDRNFSVCR
                     LNLRDHGESHHLNSGLFHGALIEETFDAVNVIAQQAGNLPVYLLGFSLGANFSLRIAM
                     RHSHTPVTNLQKVFAVSPPLDPFKTTQTIDGGPALYRSYFLRKWKRSLRKKQKFFPDH
                     YDFSGMLRARSCMELTEKMMAYFPEFSSYRAYFQLYTLTEKSFRDLGLPVKIFIADDD
                     PVIALDDFRKLRDHGFLTVSRQPFGGHCGFVDLFPQRRWYNEQIFTELNQERNG"
     gene            65151..65990
                     /locus_tag="PLA58_00300"
     CDS             65151..65990
                     /locus_tag="PLA58_00300"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOR61262.1"
                     /translation="MTMDLTLLRQKDAFPLHVLERLNRTPVDYLEKHLSIERSNAAGA
                     GHLEAGVVLLLLFRDSGFHFLLIKRSDAVAQSGDISCPGGMLERPVDEMLSHIVSKTG
                     ILRTAGNRALHELPNKDEQTLSLIQLFLMNALREAWEEIGLSPLNVEFLGALPTYSLT
                     LFTRTIYPLVCLVKDHWPYRPNDEVDKMLEIPVTAFFRSSSYALLELNTEDGKSDPRH
                     NLQFPCLVIPDGKGGEDILWGATFFIITNFLREVTGGAFPAETPGRIVTKTLSAHYTS
                     GNR"
     gene            65995..66681
                     /locus_tag="PLA58_00305"
     CDS             65995..66681
                     /locus_tag="PLA58_00305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOR61263.1"
                     /translation="MAPIPFDRSSIDTVIFDFDGTLARLNIDFEQMRREVAALMDRWG
                     VDSRSLQNRFVLEMIAEVQTLLGKNSGNHAALFTREAFRIIEEIEVAAAQSGSLFDGA
                     KGLLTGLRKASLQTGIITRNCEKAVRTVFPDIRDYCPVVICRNDVRRVKPHPEQINLA
                     LSRLSAKPGRCVMIGDHPIDIETGKNAGTRTAGVLTGRFQAEDFLQAGADLVLAEAPE
                     ILRRLKPERC"
     gene            66778..68166
                     /locus_tag="PLA58_00310"
     CDS             66778..68166
                     /locus_tag="PLA58_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOR61264.1"
                     /translation="MNPHVFREYDVRGVVDKDLNEVFIVNLGRAIGTYALQRNVRTLT
                     VGRDCRLSSDDYRRFLIAGLNAAGIDTIDIGLCATPMLYFSIRHCNAGGGVMITGSHN
                     PPEFNGFKICVGYDSIHGEQIQELRSIMESGSYLSGKGTAQAQDITRVYQDYIFDAVR
                     VSKKLKIVLDGGNGVGGKFAFPILERMGCDVIALYCEPDGRFPHHFPDPTVEENLTDL
                     IRLVAENKADLGIAFDGDADRIGVISDTGEIIWGDKLLLLFARFILKEHPGATIIGEV
                     KCSQVLYDGIGQSSGRPLMWKAGHSLIKAKMKEEKALLGGEMSGHLFFADRYFGYDDA
                     IYAAARLLEILSQTGLKLSELLADVPKTFATPEIRMDCDDDRKKAVVARIKEHYRNTP
                     GVIDIDGIRVPFGDGWALVRCSNTQPVIVLRFEASSAERLQEIRGEVERLLDGVRSCN
                     HARKSSLTEQSC"
     gene            complement(68355..68780)
                     /locus_tag="PLA58_00315"
     CDS             complement(68355..68780)
                     /locus_tag="PLA58_00315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOR61265.1"
                     /translation="MNYHLYLEKIRAGKKGLNPYLDHFDIDVVDLSPGRAVFRMPVRP
                     EYLQGAGFMQGGLMVALADEAIAHAMMTVLTPEEGLTTIELKSNFLAGVDKGKLRAEA
                     SVFKKGRSLIIGDCLVTDDGGKPVCRASATFMLLRTKQK"
     gene            69105..69797
                     /locus_tag="PLA58_00320"
     CDS             69105..69797
                     /locus_tag="PLA58_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61266.1"
                     /translation="MEHTHEVEQMCVVAKGAKNGPAPIPQEGKWTQVKEVKDISGLTH
                     GIGWCAPQQGACKLTLNVKNGIIEEALIECIGCTGMTHSAAMAAEILPHKTVLEALNS
                     DLVCDAINTAMREIFLQIAYGRTQTAFSEGGLPIGAGLEDLGKGQRSVIATMYGTKAK
                     GPRYLEMAEGYVLEIGLDGDSEIIGYKFVNLGVMMDSIVKGMDPAQALSKATGTYGRF
                     AEAVKVINPRKE"
     gene            69809..70807
                     /locus_tag="PLA58_00325"
     CDS             69809..70807
                     /locus_tag="PLA58_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HOR61267.1"
                     /translation="MALFEGYERRIKQITPFLAKYGISSLEEAEKVCLEKGVDVRKIV
                     KEIQPISFENAGWAYLVGAAAAIKKGQKTAADIAETLGEGLQSFCIPGSVADDRQVGI
                     GHGNLASMLLREETKCFAFCAGHESFAAAEGAIGLAKSANKARKVPLRVILNGLGKDA
                     AHIIARVNGFTHVATAFDYKTGKVSVAREKAYSKGDRAAVRCYGADDVREGVAIMHLE
                     GVDVAITGNSTNPTRFQHPVMGVYKKECILAGRKFFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGASVAVAVAVEQAMK"
     gene            complement(70895..71980)
                     /locus_tag="PLA58_00330"
     CDS             complement(70895..71980)
                     /locus_tag="PLA58_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR61268.1"
                     /translation="MNTILSVENLMVHRGGVRVLDIRDLKVQESRVLALIGPNGAGKS
                     TLLLTMAGLLKPEQGKIYYKGRSIETSAERSWMRRSVCVVFQEPLLLSTSVYKNVALG
                     LKFRGMTQSDIRKNVEDALAYFGISSLAKRSAKTLSGGESKRVSLARAFALKPHLILL
                     DEAFNSLDPPTRESLIDDLRHILEETKITAVLALHDREETLRLAQDVTVMSEGRLIQT
                     GATAQIFNQPQSEFVANFVGTETILEGTVQNIRSGLMEVLVSGKTIEATGSAPISQKV
                     FCCIRPENIVVSREAPENSSARNIFEAQIQKIVRQAFFYKLVLDCGFPVVAYLTVSSC
                     EDLGLCEGATVFASFKATAVHVMRREK"
     gene            complement(71977..72675)
                     /locus_tag="PLA58_00335"
     CDS             complement(71977..72675)
                     /locus_tag="PLA58_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR61269.1"
                     /translation="MELIVQGIVKAFQLLISLDPEVLGVTWLSLKISGTATFISLFFG
                     VSVGTVVALNNFPGKRLVISIINTGMGLPPVVVGLFVTIMIWRSGPFGFLEILYTPYA
                     MIIAQAIIATPIVMGISLASIQNLPPNLRLQILSLGASRMQMVWVLIKESRLPLLAAV
                     MAGFGGVISEVGASIMVGGNIKGYSRVLTTATVMETGKGNFDIAIALGIILLLLAFLV
                     NLLLTHVQQRQHPR"
     gene            complement(72682..73548)
                     /locus_tag="PLA58_00340"
     CDS             complement(72682..73548)
                     /locus_tag="PLA58_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOR61270.1"
                     /translation="MKIFKSSKRFILLAVVASLVVLGATGAFAAPKQKNIILATTTST
                     QDSGLLDVLIPLFEKETGYFVKTIAVGSGQAMKMGEKGEADVMLVHSPDAEKKFVEAG
                     YGINRRLVMHNDFIIVGPPSDPAAIKGIKSSSDALKKIAAANALFISRGDNSGTHAKE
                     KTLWKKANINPSGQKWFQETGLGMGQTLNVASEKKAYTLSDRGTYLAMRKNLGLEILN
                     EGDAALLNIYHVIEVNGAKWPKANAPGAKAFADFMVSKKTQTIIKTFGVDKYGSPLFF
                     PDAGKKVEDLGK"
     gene            complement(73571..74443)
                     /locus_tag="PLA58_00345"
     CDS             complement(73571..74443)
                     /locus_tag="PLA58_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOR61271.1"
                     /translation="MRIFVRKWSALVVLLCLVLVAGCASLSSPKSEPFIFLSSTIGPI
                     DSGIVAALEDQFEKETGIRVRHVGAGTGAALKLATNGQIDLVMAHAKSLEEKFIADGY
                     GTERIPLMYNDFVIVGPASDPAAVKGEKSATAALKKIMEANAKFISRGDKSGTHVAEM
                     ELWKKSGVEPKGAWYVVYEKGAEGNIPTLKYTDQQAAYTVIDRATFLSLKDQIKVVIL
                     VEGDEALLNYISLIPVNPAKFPKVNHKDTLTFVKWLTSPEKGQLIIRDFGKDKYGAPL
                     FFPNSVEWQKLQNK"
BASE COUNT        17175 a        20130 c        20276 g        16975 t
ORIGIN      
        1 cctgcagaaa aaccaggacg aaaagcaagg cgatcagttc tcccgccaac cggcagattt
       61 ttatatttgt cttgcgaatc atggccttat cataccggaa ggatgcttga agttcaagcg
      121 gaaaatagcc tgctgggtca agggctgttg cacaaagaca ctttgagtgg ccaatgagcg
      181 cgttttgtca cataaatctt ggcgaagcga aagatcagcc tttgacaact gtttgagccg
      241 aaggcgagtt ttgtcaaagc ctttcgcaag ccttagattt atacaaaacg ctctcaggca
      301 agcgaaaagg tgtttgggca acaagccatc ctgagctgtc ctttcgaatt tggtgtttgc
      361 tcttgacagg ttttaccgaa ggtggtaatt ccctcaacac ttttagcaca tcttgtcggg
      421 gcgtggcgca gtctggtagc gtatctgaat ggggttcaga gggccggtgg ttcaaatcca
      481 ctcgccccga ccatttttcc cagttcatct ttctcttgca atacagggaa ttttactcta
      541 caggatacgg cctgcaggaa ttgcaacatg actggttttg cagcagaatg ccgaaaataa
      601 tccgcgttga cattttgacc aatgaatata taatcccgaa gccatcacgg gcgagagtgg
      661 cggaattggc agacgcactg gacttaggat ccagcgggta aaaccttagg agttcaagtc
      721 tcctctctcg caccagaatg aaaatgaagg agaattccat acaaaagaag gagaatcaag
      781 gcgtgagcga gctttccgtt aaagtagagg acctaagcca ggtaaagaaa aaaatgtctt
      841 tcgagatccc ctgggccttt gtaaaagatg aattggacgc cgtttaccgg accatcggca
      901 agaaggcgaa aatcaagggt ttccgtccgg gcaagattcc ccggaaggtg ctggaaacct
      961 atttcaagag cgatgcggaa ggggaagcca tcaccaacat cgtcaaccgg tactactggc
     1021 aggagctgga tcagcgaaaa atggtgtcgc tttcccgtcc ggaaatcgag cagaacggat
     1081 tgaaggagga tacggacttt tctttcagcg cctcctttga aacggagccg gagattgatc
     1141 cgcagggata tcaggggatc gaactcacaa gaaccgaaat caaggtttcc gatgccgata
     1201 tggaaaagcg ccttaacgaa atccgccgca tgtttgccag cctgcaggat gtcgcggaag
     1261 accggccggc ggcgatggga gatttcgtga cgattgattt cgcgggcacg ctggatggcg
     1321 aagcgctccc cgaactgaaa gcggatgact atcttctgga actcggttca aaaagattcg
     1381 tccccggttt tgaagagcag gtggcgggaa tgaagaaagg cgagaagaag gacatttcgg
     1441 ttacatttcc ggccgattat tccgaatcga agatggcggg gaaaaacgtc gtcttttcaa
     1501 taacgctcaa ggagctcaaa gaacagaagc tgcctgaact gaatgaagaa ttcgtcaaga
     1561 atttcgacaa gtacaacacg ctggacgatt tgaaaagtga agtccggaaa tccatggaag
     1621 agcaatgtaa gcgccagagc gatgcgaagc tgaaggattc gattacggag gtcctgctcg
     1681 ccgccaatgc atttgaagcg ccggcgtccc tggtgcagag gcagatcttt tacatgattg
     1741 ccgatacgca aaaaagaatg cggtccgccg gcatggacga gaagagcgca atggaactga
     1801 gtttccgaat gcacgatcag ttcaaaaccg aagcggagaa aacggtcagg gcctttctcc
     1861 ttttcaaaaa aatagccgaa aaagaagccg tcgcggtatc cgatgaagat atggataatc
     1921 atatcaagga gttgtcggaa atccatcatg tgggtattga ttccgtcaaa agcatctatg
     1981 aggatgagga gaaaaaagaa tccctgaaag cggagattct tcagaaaaag gtatttgact
     2041 ttatcgaaca gcgggccaat attaaggtcg tggaaaagat cggaatgggc gaggaggctg
     2101 ttgcatgaat ttgatcccca tggtggtgga acaggatgga cggggcgaaa gggcgtttga
     2161 tatctattcc cggcttttga aggatcggat catctttctg ggaacggcga tcgatgacaa
     2221 tgtggccaat gtggtggtcg cgcagatgct ttatcttgag gcggaagatc cggataagga
     2281 catctttttc tacttgaatt ctcccggagg gcatgtaagc tccggaatgg cgatttatga
     2341 cacgatgcag tatatcaaac cccacatttc cacggtgtgc atggggcagg cggccagcat
     2401 ggcggcgctt ctcctggcgg cgggcgggaa ggggaaacgg tttgcccttc ctcattcacg
     2461 gattctgatt catcagccgc tgggaggttt tcagggccag gcgacggata ttgacatcca
     2521 ggccagagaa atcctcaggt tgaaggaaga cctcaacaaa attctggcgg aattgaccgg
     2581 tcagccgctg gaaaaggtga tgaacgacac ggagcgggat tactttatga ccagctatca
     2641 agcgaaagac tatggtttaa ttgatgaaat cgttgttcgg aaatccgcgg catccggtgg
     2701 taagtaaagg agatattcag tgatcaaacg aaacaaggac aatcgggggc agggtatgct
     2761 tttttgttca ttttgcggaa agacgcaaag cgaagttcgt aagctggtgg ccggcccgac
     2821 ggcatatatt tgcgacgagt gcattgaatt gtgccggtgc atcgttgaag aagacgaaaa
     2881 aacggtggaa aagggccata aaaatgttct tccggagccg aaggaaatca agcggtttct
     2941 ggattcctac gtaatcggcc aggaaaaaac caagaaaatc ctgtccgtcg cggtatataa
     3001 ccattacaaa aggctttttt ccagcgccgg ttccaacgac ggtgttgaaa tccagaagag
     3061 caacgtcctt ttgatcgggc ctacgggttc ggggaaaacc ctgcttgcca aaacgctggc
     3121 gaaatttctc aatgttccct ttacgattgc ggacgccacc acgctcacgg aagcgggcta
     3181 tgtgggagag gatgttgaaa acatcatttt aagcctcctg caaaacgcgg attacgatgt
     3241 ggcacgcgca tcaaaaggta ttgtttatat tgatgaaata gacaaaatag cgaaaaaatc
     3301 aggcaatcct tccatcacaa gggacgtctc cggtgaaggt gttcagcagg ccctgctgaa
     3361 aatcatggaa ggaactctgg ccaatatccc cccgaaggga ggccggaagc atccgcagca
     3421 ggaattcatt caggtggata cgaccaatat tctgtttatc tgcggcggcg ccttcaacga
     3481 cctggaaacg atcatttcca gacgcatcgg caccaacacc atggggtttg gcgcggaaat
     3541 caaaagcaag aaggaaaaaa gaatcgggga cgttctggcg gaagtccggt ccgaagacct
     3601 gctcaaattc ggcctgattc ccgaattcat cggacgcctg cccgtgatcg ccgcactgga
     3661 tgatctggaa gagggggcgc tggttcgcat tttgatggaa cccaaggacg ccatcatcaa
     3721 gcagtacaag aaattgtttg aactggaaaa cgtgaatctc aagtttacgg acggcgcttt
     3781 gcgctgcgtg gccaaactgt ccatggagag gaaatccggc gcgcggggcc tgcgctccat
     3841 tctggaaaac accatgctgg aagtgatgta tgatattccg tcgcagcggg acatcaagga
     3901 atgtgtgatc agcgaggaag tggtgctcaa caaggaaaag ccgattttga tctatgatca
     3961 gaaatctgaa tccgcataat ttcatacggc gcaggaagaa aaaatgaccg aagaaaaaga
     4021 cgatacgctt aaaaatagaa caaacatcat tccgctgttg ccgcttcgcg acgtggtggt
     4081 gttcccgcac atgattgtgc cgctgtttgt cgggcgggaa aagtccatcg ccgcgctgga
     4141 atccgccatg aagtatgaaa agggaatttt catggtggcc cagaaaaacg cgaagaaaga
     4201 cgacccctcc gaagaggata tttattcggt tggaacgatc ggcatcatca tccagctttt
     4261 gcgcctgccg gatgggacgg tgaaggtgct ggtggaaggc aaggcgcgcg gcgccatcct
     4321 ggaatatgtc agaaacgacg atttctttct cgttcgggtc gccgatatcg acgatgtgga
     4381 tgatgatccg acggacgtgc ggaaacaggc gctgatgcgc agcatcaagg agtcctttga
     4441 gctttatctg aagctcagca agaaagtcca cgtggagatg atgggaacga tcgccggcat
     4501 cgaagatcct tccaaactgg cggatgtcgt ggtgacgcat ctgaacgtca agctggaaga
     4561 caaacagcgg attatggaaa tcttcagcat cggcgatcgg ctggaggcca tttattcgct
     4621 gatgctctcc gaaatcgaaa ttctgcaggt ggaggaaaaa atcaagcggc gcgtcaaaaa
     4681 gcagatggaa aagacgcaga aggattatta cctcaacgag cagatgcgcg ccatccagaa
     4741 ggaaatgggc gaaaaggacg attttaaaaa tgagatcgcg gagctggaaa aacgcctcaa
     4801 acagaagaag ctctccgagg aagcgaccaa aaaggtccgc caggaaatca agaagctgca
     4861 gatgatggcg cccatgtccg cggaggcgac ggtggtccgc aattacattg actggctgct
     4921 ggacatgccc tggtcggagg tcacggaaaa caaacatacg ctcaaggaat cggaggcgat
     4981 tctcgacgag gatcactacg gcctgaaaaa agtcaaggaa cggatcatcg aatatctggc
     5041 ggttcagtcc ctggtcaaaa aaaacaaggg ccccattctc tgcctcgtcg gtcctcccgg
     5101 cgtggggaaa acatcgattg ccaaatccgt ggcgcgcgcg acgaaccgga aatttgtccg
     5161 catctcgctg ggcggcgtcc gtgatgaggc ggaaatccgc ggccaccgcc ggacctatat
     5221 cggcgccatg ccgggcaaga tcattcaatc actgaagaaa gccggctcca acaaccccgt
     5281 cttctgtctg gacgaggtgg acaagctcag ctccgatttt cgcggcgatc cctcctcggc
     5341 gctcctggaa gtcctggacc ccgagcagaa cgtggcgttc aacgacaatt atctggacgt
     5401 ggattatgat ttgtccgata ttctgttcat tacaaccgcc aatgttttgc agacaatccc
     5461 ggcgccgctg caggaccgga tggaagtcat ccgcattgcg ggatataccg agcaggaaaa
     5521 aatgcagatc gcgatcaagt tcctgctggc caaggaaatg gaggccaacg ggcttacggc
     5581 cgaatcgatc gaattcacca accaggccct gctgcgcatc atccgccagt acacgcggga
     5641 ggccggtgtg cgaaacctcg agagggagat cgcatccatc tgccgcaagg tcgccaagga
     5701 aatcgtcagc aacggaaact gcaaaaagat tgtcatcagc tccaaaaata ttccgaaata
     5761 tttgggggtt ccgaaattcc gccacggcga gacggaggaa aacaaccaga tcggtttgac
     5821 gaccgggctg gcctggacgg aggtcggcgg cgaattgctg gcgattgaag cctccatcat
     5881 ggaaggaacg ggccgcatgg tcatgaccgg caagctgggc gacgtgatgc aggaatcggt
     5941 gcaggcggcc ctctcgtaca tccggacccg cgccgagcgc ttcggtttgc cgaaaaactt
     6001 ttataaaaag atcgacattc acgtccatgt gcccgaaggc gccattccca aggacggacc
     6061 ctccgccggg atcgccatgg ctacttcgat cgcttcggcg ctcgtcaaga agaaagtccg
     6121 ggccgacctg gccatgaccg gggaaattac gctgagggga cgggtcctgc cgatcggcgg
     6181 cctgaaggaa aagattctgg cggcccaccg cggcaatatc aaaacggtcc tgattcccaa
     6241 ggacaatgag aaagacctta cggaagtgcc ccgcaatgtt caaaacgcga tgaaaatcgt
     6301 tttcgtggaa catatcgacg aggtgctgga gattgcgctg gtcaaagagg acgacctctt
     6361 cggcaacgtg gtgggaaacg aggcgcaggc ggattcctgc gccgtgaatt aacggggaat
     6421 attgttgttt taccgcttga caagccatct caatgttgct agaagccagt cagctttttt
     6481 cgggcaaggg ggcgggtagc tcagttggaa gagcgccacg cttacaccgt ggatgtcaca
     6541 ggttcgagcc ctgtcccgcc taccaggaaa gaaagtgaat attgttttta atgatttttt
     6601 ggggtcgtag ttaagttggt tataacgccg gcctgtcacg ccggagggcg tgggttcaag
     6661 tcccatcggc cccgccagaa aatctgaaag ggaatcagct ttttttaagt gattcccttt
     6721 ttgtttaccg cgaccgcccg gagaatctct tccatcagcg tgattgacag gatggatgtt
     6781 gcatggttaa ttggacgcgg aagagccgcg tggacggcgc gcggtgaaaa aatcttgagt
     6841 ctatgatgaa aaaaatttcc gatcttttat cctggagcag ccccgagcag attcgccggg
     6901 aactgttttc ggtccttgcc aaagctcaaa agggcggcgt tctggacgat gcgtccagga
     6961 aaatgatcga aaacatcctg aattttacat ccacgctggt tcgcgaaatc atgatccccc
     7021 ggacggacat cgtttcgatc agcgcggagg attcggccca ggagatgatg cgggagatgg
     7081 tcgaggcgca ttacacgcgg attcccgtcc accgcggctc cgtggacaat atcatcggca
     7141 ttctgaacat caaggacctg cttgccgcct ggtcgccccg aatgagcgcc gccgatattc
     7201 tgtctcatat gacgcggcct tactacattc cggagacaaa aaacgcgcat ctgctgtttt
     7261 acgagctgaa aaacaacaac aagcacattg ccattgtcat cgatgaatac ggcggcacct
     7321 ccgggctgat cacgctggag gacctgctgg aggaaattgt cggcgacatt cgcgacgaac
     7381 atgaagagca ggcggatgcc gacgatatcg tccgaatggc cgacggctcc atcgtgacgg
     7441 acggacgaac ggaaatcggg aagattgaag agcttctgga aatccgcttg gaccggggcc
     7501 ggttcgaaac ggtcggcggt ctgattttat acgcgattcg cagaatacct catcaggggg
     7561 aaagctttca aatcgaggga atggacatca ctatcgaaaa cgcggacgaa cgcagcatca
     7621 agaaggtgaa aatcaaaaaa tccgaagacg cgccatgaag accaacggca acaacatcaa
     7681 accggattgg accggcggca cgccgcagac ccgcgatttt tttttcgcgc tcttgtgcgg
     7741 cattctgttc tttttgtcgt tccccaggtt cggcttcggc gtcatcgcgt ggattgcgct
     7801 ggttccgctg ttcgtcgctc tgaacaacgt ttcatcgctc tcgagggccc tgcttttggg
     7861 gtggacggcg ggaatggcgg cgtatatcgg catgctgtac tggattgcgc atgtcgtggt
     7921 gaactacggt tatcttcccc tggttctggg cgtgatcctc acgattctgc tggccggcta
     7981 tctgagcgtc tatttcgccc tgtttgccgc gggaatcgtt tactttcggg gcagggttcc
     8041 cctttatctt gccgcgccgg ttttatggat ttgcctggag catttgaaat ccggtctgtt
     8101 cacgggattt ccgtgggaga acctggggta ttcccagttt tccaatctgc tgttcattca
     8161 gatggccgac atcacagggg tgcccggcct gtcctttctg atcgtccttt tgaacgttgc
     8221 cttttttacg ctgcttcggg aaaggtcgaa aaaatcgctc gcgctggcgc tggccgtgtt
     8281 tctcatctgg tcgggggtat atgcctacgg cgcgatgcgg attcgcgaaa tccggcagag
     8341 catgggaaat gcgcggagca tggacgtatc gctcattcag ggcaacatcg agcaggccgt
     8401 caaatggaat caggatttcc aaaccgaaac gattcggatt tacgaggagc tttccctgcg
     8461 cggcgcaaag gctcccggaa cgctgatcgt ctggccggaa accgccgttc cttttaactt
     8521 tcaggagccg ggggttttgc gggcccgcgt cagacaattg ccttcgcaaa ccaaaagctg
     8581 gctgcttttc ggatccgtca gttatgccgc cgcgcctgca ggcacggatt attttaacag
     8641 cgcctttctg ctttcccccg ggggggacct tgcgggaaaa tacgacaagg tgcatctggt
     8701 gccctacggg gaatatgttc ccctgagaac cttgtttccc tttgtcagca gcctggcgtc
     8761 gggcatcggc gactttgccg cggggaaggg gtatttcccc ctgcggatgg acgaaaaaaa
     8821 aatcggcgtc ctgatctgct atgagggcat tctgccccag gcggcgcgcc ggtataaaaa
     8881 cgccgcggcc gatgttttgg tgaacataac caacgacgcc tggtttggaa ggacatccgc
     8941 gccttatcag catctttcca tgtccgtttt ccgggccgtg gagacaagac tctgcctggc
     9001 gcgggcggcc aatacgggca tttccgcgct ggtggatccg actggtagaa ttctcgcgca
     9061 gacgaagctg tttgaaagag acaccatcac cggcagaatt ccattgatgc aaacgccgac
     9121 tttgtacgcc aggtacggcg attggttcgt gtgggccggc ttcatcgggc tggccggttt
     9181 attcttatgg agtttaacaa ggaggaaaag aaatgtccgt cgataatctt caggaaagca
     9241 ttctggattt gaaaacaaga atcgcctcga tcggagaatg tctttgacgt aggcgaactg
     9301 gaattgcggg tcaaggatct ggacatcctg tccgcgaaaa aggatttctg ggacgatccg
     9361 gaaaaagccc ggtccctcct gcagaaaaaa acaaagctct ccgaatcgct ggaacggtgg
     9421 aaaaggttca gcgggcgcat ccgggagacg gaaaacctct ggaacatggc ctccgaagag
     9481 aatgaccagg ggcttctgct ggatctggaa gccgatctgg cgcgcctgtc ggccgaggtc
     9541 cgggaagaag agctcaagat gatgctcgca agcgaacagg acccgatgaa cgcgatcatg
     9601 tccatccatg ccggcgcggg gggcacggaa gcgcaggact gggtggaaat gctgctgcgc
     9661 atgtatctgc ggtgggctga aaaaagaaat tttaaaacgg ccattattga ttatctgccg
     9721 ggcgatgaag caggggtgaa aagcgtttcg tttaccgtgg agggcgaata cgcttacggg
     9781 tacgccaagg cggagatcgg aattcaccgt ctggtgcgca tttctccgtt cgacgctggc
     9841 gccagacgcc atacgtcctt cgcgtcggta tttgtctatc cggaaatcga cgacgaaatc
     9901 aaaatcgaca ttcgcgacga tgatttgcgg atcgacacgt tccggtccgg cggcaaaggc
     9961 gggcagcacg tcaacaagac ggattccgcc gtccgcatta cccatctgcc gaccggcatt
    10021 gtcgtgcagt gccagaatga acgatcgcag cacaaaaata aatcgatggc gatgaaatat
    10081 ctgaaatccc ggctgtatga aagggagctt cagcagaaaa atgaaaagct cgatgaagaa
    10141 aataaacaga aaaaagacat cgcctggggc agccagatcc gctcctatgt tttgcatccc
    10201 tataagctgg tgaaggatca tcgcaccaac ctggaaatcg gcaacgccca gaaagtgctg
    10261 gacggggaga tcgatgattt tattcaggcc tacctgatgg ccgtttcgaa tcgcaaatcc
    10321 tgaaccggtt attccccgcc ggtaaatcag gagtaacatt tgcgattgtc gatatcgttt
    10381 ggggatgcgg gcaaaatgtg ttataaagcc cgcgaaaaac cgggaggcgc cggaatccgg
    10441 atgccccgcg ctttttcgtt cagggaaaca aataaaaacg cgctttgaaa acgacaggag
    10501 agccttgccc atgcgaaaat ataaaaaata caatttgtcc cgtggtctgt tgattttcct
    10561 cttcatgatc gtgggttttg gcttcgcgcc cgcggacaga gccgaggctc agctcccgga
    10621 ggtcagtgtc ctgtttcaga atccgcagtc cgtttcatcg ccggaatctc aggcgggccc
    10681 tcccgcaaat ccggccgtcc gggaagaccc caacgcggcg gaggaagact ctgcggactt
    10741 ggaagaagag caggatctga tggaaaacgt gctggccatt ctggaagtgg cggacaacta
    10801 ctggaagaac ggcgatatcg aaaacaccct caatgaactg gataaggcct atgcgctgct
    10861 tctggacacg aatggcgatg tggaaatcgc ccggcagaaa gatgacctgc gcctcctgat
    10921 ttcacagcgg attatagcgg tttactcgtc caaaaaaggc cccaccaacg gcaaggccag
    10981 tgagattcaa ctcatcatga acccggatgt ggagaaggaa atccgctcct tccagggccc
    11041 cgaaagggaa ttttttacat cctcctatct gcgttccggt ctatacaggg agctcatcgt
    11101 ttccgaactg aaaaaagcgg gcgtccccga ggaactcttc tggcttcccc tggtggaaag
    11161 cggattcaag gtcagcgcgt attcccccgc gagggctttg ggactgtggc agtttattcc
    11221 gtccacgggt tacaaattcg ggttgacgcg ggacgaatgg gtggatgaac gcatggacgt
    11281 cctgaaatcc acgcgggcgg ccattgctta tttgaaggag ctgcacgcga tgttcggcga
    11341 ctggctcacc gtgctggccg cttacaactg cggcgaaggc agggtcctgc gcgtcatttc
    11401 cggccagcac atcaattact tcgaccgctt ctgggatctg tattcccggc tgcctcatga
    11461 gacggcccgc tacgttcccc gttttctcgc cacactgcat atcgtcaaaa atccggccaa
    11521 atacggcttt gagctgcaga ccacgacgga cacgctgatg aactatgaaa cggtgaaggt
    11581 cggcaaaatg atgaaactgt ccgacatcgc ctccaaaatg gaagtatccg aagactacat
    11641 gaatgtgctc aatgcggagc tgcgatacaa aatcacgccg gacagggaat atgatttcaa
    11701 ggttcccgag ggcagtcttc aaaaatttca actggtgtac aacgatattc cggccgccga
    11761 gaagccccgc ctcggttacg cggagagaaa caaaaacaca ggccccggcc gctacatccg
    11821 gcatcgcgtt cgccccggtg aaacggtgga ctccattgcc aaacgctaca gggtatccag
    11881 acagtcgatc tacagctgca accggatcaa tcgaaacaag cggttaagcc agcaggcgaa
    11941 atatgtcaga ataccgattg attccgcgga aacggcgtca gccaaacaaa aaacaaccgg
    12001 cgggaaaaac gcttcttcgg ccggttcagc caagacgtac aaagtcagga aaggcgacac
    12061 gctgtttagc attgcaacgc gtttcaacgt ccccgtcgcc aaactcaagc aggcgaatcg
    12121 tttaacgacg aatgagatca aatacggaac aacgctgaaa attcccgcgc agggatgacc
    12181 tcaggcaaac ggggcgtcgg aggccgtcag ggccaggaaa agctcttttt ctcttttctt
    12241 cattttcagg gcgttggccc tggaggttcc aacgtgctcg taaccggtca gatgcagcag
    12301 gccgtgaatg attagaaaga gaatctcctc ctccagggta aaaccgcctt gctttgcgtc
    12361 gcgcagggcc gtatccacgg aaatcacgac atctcccagc aggccgggat ttatcttgcc
    12421 gcactcgcct tcctcgagtg aaaaagaaag cacattggtc ggtttgtcca ggcgcagata
    12481 ctgccggttg agaaggcgaa tggcctcgtc atccacaaac gaaatgctga tttccttttc
    12541 cgcgcagccg atcagccgca acagccggat gacgttgccg cggatccggc ggctatcgat
    12601 tttcgtccgc ttctgccggt ttgcgattcg aattttcata aacttctctt ttgccgttcg
    12661 tttctttcgg cttcgggggc gcttccggat attcgacgcg atgatggaaa atgccggtta
    12721 aaatccgcgt gaaggtttcc gcgatggtat tcaggtttct gagcgtcagc tcacactcat
    12781 ccagctgccc gtccgtgtaa atgcgggcga ttctttcccg gaccagcgac cggattcggg
    12841 ccggcgtcgg gttggtcagc gtgcgcgagg aagcttcaat cacatcgccc agcatgacga
    12901 gccccgcttc tttggtctgc ggtttggggc cgggatagcg aaagtcgctt tccgtcaggg
    12961 agcggatgga ttcatccttg tttttcttgg ctttgtcgta gaaatagctg atcagactgg
    13021 tgccgtgatg ctcccggatg atgttgatga tcggctggcc cagcttggcc ttcatggcca
    13081 gttcgcagcc ttccttgacg tgggaaatga tgatcagggc gctcattttc ggagaaagct
    13141 tgtcgtgtcg attgtcatag ccggtctgat tttcaatata gtaatggggt ttggcaagct
    13201 tgccgatgtc gtggtagtag gcgctgacct tggccagaag cgcgttggcg ccgatggctt
    13261 cggccgccga ttccaccagg gaggcgacga tgatgctgtg atggtaggtg ccgggcgctt
    13321 caatgatcat tttctgaaaa agcggctgat tgagattggc cagctccagg agcttaatgt
    13381 acgtgatgaa gccgaagagg ctttcaaaaa cgggcgtcag accggccacc aggattccgg
    13441 tgatgatgcc gccgaaaagg cccatggcca gacggaggat cagatcgtcg agcagccggc
    13501 cggtcaatag attcaggctg aggatggcgg caatgttgac gacgcccaga aagacgccga
    13561 ccttcaggaa cgtcgagcgt tggcggctgt tgacgacgtg ataggaggcg gccacggacc
    13621 ccagaaacga aaaaagcggg aaaagaattt tttcctcaaa aagcagactg atcagaaacg
    13681 aggatagaac gctcatgatc atggccacgt tccggttcac cagcacggcg ataatcatgg
    13741 cgcccatggc gaagggaatc gcgaaatagc atgcgtccac cggaagggaa gggaaagccc
    13801 ggttgacggc gaccgaaata aaaatgcccg ctttcacaaa taaaatctgc agcagggcga
    13861 cgatcgcgaa aaccagaaag tctacattgg agcggggcga tgttttcagc cagtttcggg
    13921 tgcgccagaa atacaaaagc agcgtcagaa agagaacgat gcagaaaatg cccgcaagga
    13981 ccgcgaggct ggagatgttg ctgaccgcgg cggttttgtg aaaggcttcc agcttggcca
    14041 gctccggata accgattttg tctccctccc ggacgatcat ctcgtttttc tgcacctgga
    14101 agtaaaccgg cttgacgctt tctctcgccg atgcaatttt ttgcgccgtc gccttcgggt
    14161 tgtaggtgag attgggcgcg atcattttct tcaggaataa aaatgtaacg cgtctggcct
    14221 ccggctgttc gtttcgcatg gaagcgttga tttttttcga aagcgccggg ccgatgtctt
    14281 tcatcagcag aatggacgac aactctttgg tgttttcttc cgtccgggtc agcgtgttga
    14341 cgatggtaat gcctttttca cgctcctgat tcccgaaagg gcccgtcgtg acgaaggtgt
    14401 tttcgtaaaa ggcaaaaatg attcgggaga atctctgcag cagctcgtcg gaaaaagcat
    14461 gggcgctcag ataagcgaac tcctcctgcg acagcgtgac gcccagggac cgatccagaa
    14521 gagccttgac ctgagatgtt tccctctgcc cggccgcgag cgcaaaggac tgtttgattt
    14581 tcgcccttat cgtcatggcc gtttccggat catagtcata taccggacga acggcggcag
    14641 cggattcgag ttgctttagc cttgtggatt ggacgtcctc caccaggaag tcgcggtcgg
    14701 ctttgacgtt ttcctgaaca atcatgccgt ggcggtacac cggcttggaa gaatagaatt
    14761 gcggcgcgag aagcgccgtc aggccggcgc tcaaaaggac ggcgatcacc cagcgctgaa
    14821 cgatgacatc gtttaaaacg gcaaaggaga agcgatgctt ttccttgcgt ttccgaaaaa
    14881 aagacagtat tttttccgat gtcgaatcca aaaaattatt catggccgcg cttcattttt
    14941 cccgtttttc ttttcgtttt cccaggctgc ataggcggag atcacgtccg ccaccagggg
    15001 atggcggacg acgtccacct gtgaaaaatg aacgatgcgg acggcctgta tcttgcgcag
    15061 caccgccgcg gcttccacca ggccggagga tctctccatc ggcagatcga tctgggtgat
    15121 gtcgccggta acgaccgcct tggaatggaa gcccagcctg gtgaggaaca ttttcatctg
    15181 ttccgacgtg gtgttctggg cttcatccag aatgatgaat gagtcgttca gcgtccggcc
    15241 gcgcatgaac gcgagcggcg cgacctcgat gagccccttg gcaatcaggg cgccggcttt
    15301 ttccatgtcc accatgtcat aaagcgcgtc atacaagggg cggaggtagg gattgacctt
    15361 ttccgccaga tcgcccggca aaaaacccag cctttcgccc gcttccacgg cgggccgggt
    15421 caggatgatc cgctggacct ttttttccag aagacaggag acggccatcg ccatggccag
    15481 gtaggttttc cccgtgccgg ccggtccgat ggccagcgcc atgtcggcgt ttctcatggc
    15541 gtcaatgtaa tggcgctggg cgatgctttt gggagagatc accctctttt gggaagagat
    15601 gaaaaccgtc tccagaaaaa tactcgcgag gtcaaaatgg atgtcttccg agagaatccg
    15661 gtgcgcataa tcaaaatccg agaggtggac gcgccatcct ttttgcatca tttcatagag
    15721 ttgccggatg atgcccgaag cgattgctac ggctgtgtct tcgccggtga cagacaagca
    15781 attcccccag ggcttaattt tgacgggttc aagcttttcg aggagtttca ggtgcttatc
    15841 gtgttcgccg caaagatttt tgagcagatt tgaatccgcg aaggaaatct tttccgctcg
    15901 tacgcttttc gttgacttaa tcaaaaaaat ccacctttaa aaggttgatc tccgccattg
    15961 cagagccgtc gtatccgggt ttttgccaat ggcaatggct ttttgcgcga ggctggtttc
    16021 taccattaag aacccgctat tgcaatacaa tttaagcctg caaaaacagg tgggcaaaga
    16081 ccttggcgtc gccggtcatg ttgccgatcg ggcagttttc gtcgggctga tgggctttca
    16141 tgttggtttt tgaccagacc gccaccgaaa gcccctgctt cctgaaatac gcggcgacgg
    16201 ttcccgcccc gacgcctccg gcaaacgccc ggatctgata gacccggttg acggcgtccg
    16261 tgagggcgcg gacaaccgga gcatcggcgg gtgtggaagg cgctgaatca acgcgctggt
    16321 gaatggagat ctcgatggtt acacgatatg ccgcttcaat gcttttggtg atttttttaa
    16381 tttcggaaag aacgtcgtcg atcctgtagc acggcaggat ccgacaatcc atgtagaaga
    16441 cgtcttcacc cggaatcgtg ttgatgttgc ccacgttggc ttcttttttg gtcggctcga
    16501 aggtgctgac gggaggatcg aaaagcggat ccgcggcgtc aaagattttg cgcagttcgg
    16561 tcaattcgat gaccagacgg gaggcggcgg agaacgcatt gatccccagc tgcggtttgc
    16621 tgccgtggca ctgttttccg gtggtcctga aacccagcca gaggattcct ttttcggcga
    16681 cttcaatcag ggatccttcg gaatttcccg aatccggcgc cacaatccag tcgccggggc
    16741 tgaaaatttt ttccggcaga ccgagaagat gctgcacgcc tttttcgctg gacgtttcct
    16801 cgtcggagac aaacagcagg ttgaccgtgg cagggggcgg gatgccttcc tccaagagcg
    16861 ccctggcggc aaaaacagaa gccaccatat cctgctgatt gtcttccgtg ccccggccgt
    16921 acacgcaatc gtccttgacg tacacccggt agggatcggc gctccagagg cccggctcgc
    16981 ccggagggac aatgtccaga tgcgtaatga tccagatgcg gcgggagtcg tctttccccg
    17041 gcagcgatgt caggaaattc gggcgcagac cgcccgaaac cgtcgggtcc ggggcgtcaa
    17101 aatgccggaa aatcgtaaac cccattttca ccagacgctt tttcagcgcc agggctttcg
    17161 gcatttcacc atcgccgccg tttgccggac cgacggccgg aacggccgtc agcgcttttt
    17221 gcagttcgat catgtcctgg cggctctggt cgattctgcg ggatactttt tcaaaaagaa
    17281 cttcgtcgat catgatcaaa cctcgaatca gtgctgggga acgaattgcg aaggcggggc
    17341 ccagttttgg ggccatgcgc cgaaccggtc cgcgtcgccg acgatcaacg tcaacctttc
    17401 tttctcatgg aggaaggccg cggcgacgcg gcggacatct tcgagcgtca ccgcttcgat
    17461 ttttgttcgg taggccgtca ggaaatcggc gggcagcttc tcgtattcga ccgtcatttg
    17521 ctgggaggca atctgtccgg gctgttcgaa ggagaaaatg aaactgttga tgatcgatcg
    17581 cttcgcccgg tccagctctg tctgggaaaa ggatccggcc gcggcatcct ttaaaatggc
    17641 gtcgatcagg gaaatcgccc gaaaggtcga agccgttttc gtgaaagcat aggtcccgaa
    17701 aaccccgtag ttggaccgtg cgcggtagaa gctgccggcg ctgtaggcca ggccttcctc
    17761 attgcggacg gcgctgaaaa tccgggagga aaagccgccg ctgccgatga ggaaatccag
    17821 aacctcgaaa gcgtaaaagt ccggatggtt cttgctgatc gtcagctccc cgctgaccac
    17881 cgtggattgc ggtattgcct tgggaatcag aaaaatgccg cgccccctct gtttgggcgc
    17941 atccgggagc tcatggacgc ggcgggggat ggttgcgtcg ctgaaggctt gctcgatttt
    18001 ctctacggcc tcttcccggg aaatgtcgcc cgacaccgcc agcaacatat tggccggcga
    18061 aaaatattcg cggtgaaaag ccgcgagatc ggcgcgcgaa atgttttgta tggaggcgga
    18121 ggttgcatag cggccccgcg gatcgccggg atagagaagt cggttgaatt cgcgaaaggc
    18181 cagtttttgc gggtcgtccg cgattcgcct caactcctcc ttttttaacg ccagcgcctc
    18241 ctgaattttc ttttcttcaa aggccggctc cagaagcatc cgggagagaa ggccgaggca
    18301 ttcctccaga tcgcttttca aaaaagaaaa atgcaccgag gcggaatcaa gcgacatcga
    18361 aaaggacgga gaggcggcca ggaaatccag tcgccggtcc atttcgccgc tgctgatttt
    18421 tgttgttcct cccgtccgca gaagagcggc ggtaagctcc gccagacctt ccttgccctc
    18481 ggggtcgtag agcgagccgg ttcgcaccag cacggtcagg gaaaccagag gcaattcgcc
    18541 gtctttcaga tagaacaaaa ccatgccgtt tgtgagtgcg atccgctggg cctgcggcat
    18601 gcggaattcc agcgcgggat aggcgatttg gtccggagaa atccagggag attctgcggg
    18661 agccgacggc ggactcatcg cgaaaatcgc caggagcagc aacccggcgg ccgggaaaaa
    18721 ggcttttcta tttttcatgg gcgcctccgc cttcctccgg tgcggttttc cggttgagaa
    18781 ccgcgacggt gcggttttcc gccgtcagat accggatggc cgccgcctgg atatcaccgg
    18841 ccgtcacctt ttgaatttgg ggcagatagt cggaaaagta ccggtaatcc cccaacagca
    18901 gttcggtgta ggaaagaatg gacgccagct cggaattgga atccaggctt ttgatgaagt
    18961 ccatcttcag ctggtttttg gcccgggcca gctcctcttc cgtcacggga gcggtcttga
    19021 tcgcctcgat ctccccgaga atggccgtct gaagttcttc gttggtatgg ggatggcgcg
    19081 gccgcgcaaa aatggtgaag aggttggggt atcttgctgc cggcacgccg tttgccgcgc
    19141 tgatgctctg ggcgatctgg cgcgaaatca ccagccggtc atacagacgg ctcgttctgc
    19201 ctttgcttaa aatcgtctcc agaacatcaa acacataatc ctcgcgggcg ggcggcgcgg
    19261 gcttgtggta gccgatgatc atggaagggc tggcgtcaaa ccagacctgg gctttgcgct
    19321 ccgctttttg ctccggttcg gccggaatcc gggcggcagg agcgccggcg tcggggatct
    19381 ttccgaaata ccggcgaatc agctccatcg tcttggaggg atcgatgtcc ccgaccacgg
    19441 caatgacgat ccgccggggc gacagatatt tccgatgaat ggcgtgaatc gccgccgggc
    19501 tcatgaacgt caggtctttc ggcaatccga caatggggat tccgtaggga tgggttgaat
    19561 aggcccgttc cataaaggcc tcatagagct tgccgtcggg gctggcctcc acgcgctgcc
    19621 ttctttcctc cagaatgacg tcccgctccg tgtaaaactg acggaagacg ggatgcagaa
    19681 ggcggtcgga ttcaatgcgc gcccagagtt cgatcttgtt ggcgggaagg ttgatgaagt
    19741 aggtggtcat gtcctggccc gtcgacgcgt tcatgccgga tgcgccgttt tccgtataga
    19801 ggcggtcaat ttcgttgggg atgtaatacc ggaggtgctt gtcctggagc cttttcatcc
    19861 gcgcttccag ccgccggatc ttccgggcat cggccgatcc ctgctttgcg cgctcgcggt
    19921 ccagttcccg gccgattttt tccatgtccg aaagcagttt cttttccgcc gggtagtttt
    19981 tcgcgccgat cgtcgtcgtt cccttgaaca tcatgtgctc caggaaatgg gccgcgccgc
    20041 tttgccccgg ggattcatcg acggcgccca cccggtagcg gatgtacagg gaaacggtcg
    20101 ggctgaattt gcgctccatc atcagaacgg tcaggccgtt ttgcagcctg gccttcacga
    20161 tttttttctc caggtccacc gcctgcgcag gcagggcaat gcacgcgagc aacagcagcc
    20221 cgagggcgat ccagatttta ttctttgtcc gcatggtcct cctcagaaat cgaatttccc
    20281 tggaaaaaac gaaaatatgc gtagagaatg ccggcgatgc aaaacaaaat gatgaaagag
    20341 gccgcaaaca gcgtcatcac gaattccagg ggattggcca gctgaaagga gctcatgagg
    20401 agaagaacgc cgaacaccag aaaaaatccg gagagcaaaa aaacgacgat attcttaatt
    20461 cgaaccagta gttccatcgg gcaatcctgt ttcccagggc agcgctttga cgcgctcctt
    20521 catcctgcgg taagcctcat aactgaaatc attattgcgg cagcacaaca gtgtgttgtt
    20581 atgatataga ggttcccgct gaatgtcaaa tacggatgcg gcgacggctt ccggccacat
    20641 tttagtgccc ggaatcggcg aatattcggc cagcacgggc cttgcgccgc accgaaggac
    20701 gaaatcaatg ctcccggaca catcccctgc tttctgatcc ggcaggccgc acaaaaggta
    20761 aacgccgatg tcccgggttt tgtaccccgc ctctttcaaa tgcgcgaccg ccctgcgcaa
    20821 ctcatcgttt cgcacctttc cgcccgtttt ctcctgcgcc tcgaaacggg ccgtttcaaa
    20881 accgaagcga atggttttaa agccggacgc aaagagcaga cggctgagcg gggcgtcgat
    20941 ttcccggaca tgcaggccgt tcgggcagtg aaaccggcag tcgatgccgc gcctttttat
    21001 gtcgtgaagg agtggaacaa tactgctttc ggggttcacc agaagcgcgt cgtcgtaaaa
    21061 actgaaatcc gcaacaccga aattcttatg ccagtattcg atttcgtcgg cgatttttcc
    21121 gggatcgcgc cgcagatgct gtttgtaaaa taaaggggac gaacagtagc tgcagcgata
    21181 agggcacccc agagaggtga tgagcgggac ctggtcgatg cggtccgcga ggtcgaaggc
    21241 gggatagggc agggcgtcca gatcgttttc ctccggagaa aaaagaatct cttcgttgaa
    21301 aataccgcgc aacagctcca gaatctgttt ctcaccccgt ccgctaatca cgtaatccgc
    21361 gccggattcg gccagggcat gctcatggca gagagaggcg tatttgccgc ccaggaccac
    21421 cggaaccccc ggcagggttt cccggacgat ttgaatggcg tcaaaaacgc ccgggtacca
    21481 gtaagtcatc atggaagtga ccaaaacaat gtccgtgtcc gaaaaacgcc cgagttcggc
    21541 gcgaaagacg tccggatcca tcccataccg gcaatagctg cgtggaaacg ctttgaggca
    21601 ggacggcttt tcgatgacct gtcggtagaa gcggccatgc ccgtaggcat gacgctttgg
    21661 tgacctgccg gtcttcttca gcatgggctc tgaataggga ttcaggcaat cgatatagga
    21721 aacccggtgg cggttggccc gcagaaggga gcccagggtt aaaagcccca gaggtttcat
    21781 ccagaaatcg tacgccgcga aatcataaat ccagggattg atcaacagaa tatttttgct
    21841 catggatgcg tcttgtagca tacccgggca aggattacaa aagcttggac aagttcatga
    21901 atttccacgg aaatatttta taacagaaag aacttgaaca ctcggggaat tgttgctaac
    21961 atgcgcgcac ttttaaatgg gatgccttat gcgtgcagtt cttcagagag tcgaccgggc
    22021 ctgcgtcagc atcaattctc agctattttc cgctacggat caggggcttt tggtgtttct
    22081 gggcgtggaa aagggagaca cggagaaaga cgctgatttt attttggaaa aaacggttca
    22141 tctgcgcatt tttgaggatg aacagggaaa aatgaaccga tcgcttgtcg acatcgcggg
    22201 agaaatgctg gtcgtatccc aattcacgct gctggccgac tgcgacaagg gacgaagacc
    22261 gtcttttgcc cgggcgcaag agccggaaaa agccagagcg ctgtatgaat attttgtccg
    22321 ccgggctcaa ccaagggtca aaaaactggc caccggcgca tttcaggaaa tgatgcagat
    22381 tgaattggtc aattccggcc cggtgacgat tctgctcgac agcaggaaaa tggcgtagaa
    22441 atggatgaaa gcgacatcaa aatagacaag gaaggaatct ggtactaccg aggcgcacac
    22501 atgttccgca aagacatttt gtgcgttttc ttcgagcatc tgaaacagga tgactgcggc
    22561 aggtatttcg tcgaactcaa ccgggaaata tgctatctgg atgtcgaaga tacggccttt
    22621 gtcgtgacgg cggtttacag gaatcaaacc ccctgcggcg ctgaagagct ggaaatcctg
    22681 ctcaacgacg accaaaagga gaaactggct gcggacaccc tggccgtcgg caaagacaat
    22741 gtcctgtatt gccgtgtaaa aaacaaccgg ttccccgccc gctttacacg gaaaagctac
    22801 tatcagctgg cggaatttat tgaaaccgac ggcgcggaca acaaattctg cgtcgttttg
    22861 aacaaccgaa aatatccgat cgcacacaca tcccttaacc catgacaacg gaggagacca
    22921 tgttagatga tgttgtgaaa gaatccctga catttgacga cctgctgctg gtgccggcgg
    22981 cttccagtgt tctgccgcgg gacgttgaaa cggccacctg gctgacgcag aagatccggc
    23041 tgaacattcc catcgtctcg gcggccatgg atacggtgac ggaatcccgc acggccatct
    23101 gcatggctca ggaaggcggc atcggcatta ttcaccgcaa catgagcatc gaacgccagg
    23161 ccatcgaagt ggaccgggtg aaaaaatccg aaagcggcat ggtggtcgat ccgatcacca
    23221 tcgagccgga gcgaaaagtc agcgatgcgc tggctctgat gcaccagtat tccatttcgg
    23281 gcgtgcccgt cgtcaaaaat gaaaaactgg tcggcattct gaccaaccgc gatctgcgtt
    23341 ttgaagaaaa tctggagcag cccgtgtccc aggtgatgac caagaaaaat ctcatcaccg
    23401 tcaaatccag cattaccctg gaagaatcca aaaaactgct ccacaagcac cgcattgaaa
    23461 aactgctggt cgtggacgat gaaaaccgtt tgaaaggcct gatcaccatc aaggacattg
    23521 aaaaaatccg ccggtatccc aacgcctgca aggactcgct gggaaggctg cgcgtcggcg
    23581 cggccgtggg catcatcgac cgggagccgc gcatcgaggc gcttttagcg gccggcgtcg
    23641 atgtgattgc cattgacacc tctcatggac attcggcggg cgtgattgac gccatccggt
    23701 ccaccaaggc gaatttcccc aaatgcgaac tgatcgcggg aaacatcgcg acggccgagg
    23761 gcgcccgggc gctgatcgag gcgggcgttg acgcggtaaa ggtcggggtc ggcccaggtt
    23821 ccatctgcac cacccgcatt gttgcggggg tcggcgtggc gcagatgtcc gccattcgcg
    23881 acacgtacaa agtcgcctct caaaaaggaa tccccgtcgt tgccgacggc ggcatcaagt
    23941 attccggaga catcgtcaag gcgctgggct gcggcgcgaa tgccgtcatg atcggagggc
    24001 tctttgccgg aaccgaagaa agccccggcg agacgatttt gtttcagggg cgcagttaca
    24061 aggtatatcg cggcatggga tcgctggagg ctatgaaaat gggaagccgc gaccgctatt
    24121 atcaggacga catcgaagcg ccgtccaaaa cggtcccgga agggatcgag ggcagggtgc
    24181 ctttccgcgg atcgctttcc gccagtatcg tccagctcat cggtggatta aaagccggca
    24241 tgggctacgt gggctgccga acgatcgcgg aactgattga aaaatcccgc tttgtccgca
    24301 tcacgtcggc aggcctcagg gaaagccacg tccacgatgt catcatcacc aaggaagcgc
    24361 ccaattactg gattgattag tcaaacatga agcaagccga tttcgtccat ctccatgttc
    24421 atacacagta cagccttctc gacggaatga tccgtctgga cgaactcttt aaaaaagcca
    24481 aagaattcgc catgccggcg atcgccatca ccgaccacgg cacgatgttc ggcgcgattg
    24541 atttctacaa acaggcgctc gccaacaaca tcaaaccgat catcggctgt gaactgtatg
    24601 tcgcgccccg gagccgcctg gacaaaacac cgtccgccgt cggcgacacg gcgcgccatc
    24661 tgatcgtctg ggtcaaaaat ttgcagggat ataaaaatct gttgaaactg acctccgtcg
    24721 cccatctgga ggggttttac taccgcccgc gcgtggacaa ggccctgctg gcggagtgct
    24781 ccgaaggcct gatcgcgtcc agcgcctgcc tccacgggga aatcgcttcg catattgtcc
    24841 ggggccatat ggaagaggcc cgcagggccg cccgtgaact tcagaaaatc ttcggcgagg
    24901 acaatttcta tctggagctc atggaaaacg gcattcccga gcagaagatc gcgaaccagg
    24961 gcttgatgga actgagcagg gatctttcca ttccgctcgt tgccacaaac gactgccact
    25021 atctggaggc cgatcacgcc gaggcgcacg atgttcttct ttgcattcag atggggaaaa
    25081 cgatcgagga caaggaccgg atgtcgatgt cgtcgaatca gttttatgtc cgctctccgc
    25141 aggaaatgca gacgctcttt gccgcgaccc ccgaagccgt cgccaatacg ctggccatcg
    25201 cggagagctg caatctgacc tttgaattcg ggaaatttta cctgcccaaa ttcgaggtca
    25261 aaaatcccga ggaaacgctg gaggattacc tggaccgcaa ggcgacggaa gggctggaga
    25321 aactctttcc ggtcattctc aagtatgcga aggaagacga ggcaaaagtt cgcgaaaaat
    25381 acacccggcg cctgcgctcc gagcttgcca tcatcaagaa gatgggcttt gccggatatt
    25441 ttctgatcgt ttccgatttc atcaaacacg ccaagcacac gggcgttccc gtcggtcccg
    25501 gacgcggctc cgcggccgga tcgctggtgg cctacgccat ccgcatcacc gatatcgatc
    25561 ccctgcgcta cggactgttt tttgagcgtt ttctcaatcc ggaccggatc agcatgccgg
    25621 atatcgacat cgatttttgc caggagcgcc gcggtgaaat catcaaatac gtcacggaaa
    25681 aatacggcaa ggacaaggtt tcccaaatct gcactttcgg caagatgatg gccaaaggcg
    25741 tgatccggga tgtgggaaga gccctgaata ttccttacgg cgatgcggat cgcattgcca
    25801 aactggtgcc ctcattcccg ctgaacatca cgcttgcgga agcattcaag gcggagcccc
    25861 gctttgccga agagcgcagg aaaaatccaa aaatcgacaa gatgctgtcc ctttcgctga
    25921 ttctggaagg cctcaaccgg cattcttccg ttcacgccgc cggcgtcgtg atctccgatg
    25981 ctccgctcgt cgagcgtgtt ccgctttttg cccccaagga cgatatagtt tcccagtatt
    26041 cgatgaagga tatcgaaacc gtaggcctga ccaaatttga ttttctgggg ttaaaaacgc
    26101 tgaccgtcat caaaaacgcc gtggatttca tcagggcctc tcataacgtt gaccttgacg
    26161 tgaataacct tccgctcgat gatgaagaaa cctaccggct gctgtccaga ggggaaacag
    26221 acggtgtttt ccagttggaa agcgccggca tgaaagacct gctggtcaat tttaaaccgg
    26281 accgcattga agacattatc accctgatcg ccgcttaccg gccggggccg atgcaaatga
    26341 ttcccgattt tatcgcccgc aagcacggca agcagcaaat tacctatgag cttccccaac
    26401 tggagccgat cctgaaggaa acctacggaa ttattctgta tcaggaacag gtcatgcaga
    26461 ttgccaacgc tatcggcggc tatacgatgt cccaggccga tacgctgcgc aaggtgatgg
    26521 ggaaaaagca aattgcggcc atggagaagg agaaaccccg ctttctggaa ggcgcgaaaa
    26581 agcaaaacat caatgaaaac aaggcgaaat acatctggga gcagatggag aaatttgccg
    26641 cgtacggctt taacaaatcg cacagcgccg cttatgcgat gatcacgtat cagaccgcct
    26701 atctcaaggc gcattatccg gtggctttcc tggcggcgct tctgacgagt gaaaaagaca
    26761 accgggacaa gattatcaag cacatgtcca actgcaagga aatgggcatc gatattctgc
    26821 ccccggacat taatgagtca cagaaggatt tcagcatctc gggcggcaac atccgtttcg
    26881 gccttaccgc cgtcaagaat gtcggggaag cggcgattga atcggtcatg gacattcgaa
    26941 aggagggaaa atttacctcc tttatggatt tcctgtgccg tattgatctg cgcaaagtca
    27001 accgaagagt actggagagc ctgatcaaat gcggatcttt tgattctctg ggctacacca
    27061 ggcggcagct tatggaatgc ctggatgcgg ccatggaggt tgcgcagcgc aaacagaagg
    27121 aaatcctcag caatcaggca agcatcttcg atcagctcgg cgctgaggag agagcggaga
    27181 caaaaggtag cggatctttc tccatccccg atcttcccga atgggaccgc aaggagctgc
    27241 tcgccatcga aaaggaaacc gtcggatttt atatttcggg ccacccgctg cacggctacg
    27301 ctggaaaatt aaacctggtt gccaataccg attcttcgac tctgaacaat aaaagggaca
    27361 aagaaaccgt caccattgcg gggattgtca gttcgctttc cgaaagactg accaggaaaa
    27421 aagacgttat gtgcaacgtc gttcttgaag acctgcaggg ttccgccagc attattttct
    27481 gggccgatat ttacaggaaa tattcggaat tgctccatgc cgacgaaccg gttgtgatcc
    27541 agggcattgt cgatgtcgga gacgaatcga acaaaatcat tgcccaggag gtgataccgt
    27601 tggccagggc tctggaaaat ccctacaaac aggttcgttt tatagtccgg acggacaaaa
    27661 tcccaccgga gggaatttcc gcgctgaacg ataccattcg aaaatacagt ggaaaatacg
    27721 agggttatat tcatctgttg aacgggaaat gtgaaacgat cgtccgtctg ggggaccgga
    27781 caaaactgga tatctgcgag aaactccgac gggaggcgga cgcgatcctg ggggagggta
    27841 gtacggttta ttgttaaaag aatgggcaat gggcgatagg aagaaaaaga tgagaaacac
    27901 gtgacgagta atgctaatat gatatatgag gaaagaacat ataggtcact aattaataaa
    27961 gataatctga tttcatatca agttaaagtt gctcaaagtg atctgtggat aagttccgat
    28021 accgatctgt ctggtcttgc tgaaaaatct cttcgacaac atcgatattc actggaatcc
    28081 tatatccaca gatatccgga attccgtacg acacttcttc catttccgca agaccctctg
    28141 gctcctgcga tcatcaggga aatgattgaa caatcaactg tctgcggggt agggcccatg
    28201 gcctccgttg ccggcgctat cgcggaattt gtagggaacg atctggccgg tctcacggat
    28261 aatatcattg tcgaaaacgg aggagatatt tttcttcgat ccaagaaaag tctttcgata
    28321 ggggtttttg ccggagaatc aactttaagt tataaagtca aactaattat aaaacctgat
    28381 gaaacgcctt tggggatctg cacctcctcg gcaaccgtag gtccgtcttt gagctttgga
    28441 aaagccgacg cggtctgtgt tctttcccgc tgtgcaaccc tggccgatgc ggctgcctcc
    28501 gctgtcggca attgtgtaaa aagcaaaaac gatataagga aagcgctcga tttcgggttg
    28561 aaaataccgg gggttcgcgg cgtcgtgatt atcattgaaa atgagatggg agccgcagga
    28621 gaggtcacat tcttctgaga aatggtaaaa ttcggatgag tccaggaaaa cgaggtatga
    28681 tatatcgata tgaatgaatt tactgaagaa atatggaaga gaagcccttt tatcaagctg
    28741 ctgggacttg aaatcgaaaa gatcgaacac ggagcgtgct tcagtcgcct gcggatcaga
    28801 gacaccttgc tcaacaagca cagcgtcgtt catgggggcg tcatttattc catggccgac
    28861 atcagtatgg gagtggcggt ttattcgaca ttgaaaagcg gtgaagaaac atcgacggtt
    28921 gagataaaaa tcaattatct gaaacctgca aaaaccagcc ttttaacgtg tgaagccaaa
    28981 atattgaaga aaggcaaaaa cattgccgtg atggaggcgg atatccgatc tggtgaaaat
    29041 tcactgattg ccaaggctct gggtacattc atgtttacat aacatatctt ataagacata
    29101 atgttaaaat atttgtcgtc agcagagaca taaaaaaaga cctgctaccc ttcaaccttc
    29161 agtcttcggt cttcagcctt aaaacctatt atattccatc cgccgcaagc tgctcaatca
    29221 tacgaatctg cttgtcggtt agtttcttcg gaacctcgac gttgatcttg acgaactgat
    29281 ccccttttgg agctcctttc attcccggca ctccaaaacc cttcatgcgt attttggtat
    29341 tgttttgtgt tccgggtgca atcttcaatc tcttggtgct gccgtcaagt gtgggaacgt
    29401 cgatggtggt ccccagagcc gcctggctaa atttaatggt tttttcaatg taaagatcgt
    29461 ttccatcgcg ggcgaaaatc ggatgaggca aaacattgat gttcaggtaa agatccccgt
    29521 ttggaccgcc ctgatagccg gtcaggcctt ttcccggaag tctcagtttc ttacccgtgc
    29581 tgatgccggg gggaattttg acattgatat cttcaatgcg gttttccatt tggagcgaaa
    29641 tcttcttgtc ggcgcccata acggattctt ctaatgatat cgataaatta tattctacat
    29701 cctgtccctt ttgcggcata tcggcgtacc gccggcctgc ggcgcctccg cccatttggc
    29761 cgaacatgcc gtcaaacgga tcatcatatt ccgtatagcc gcctcgcctc gttcccgtgc
    29821 ggaaggttgt tcgtccccct cccctggagc ctccgaatcc gaagctgcgc agaatttcat
    29881 ccaggtcaaa tccgcggaaa atatcttcct gtgaatactg ctggcgaaac tgatcagctg
    29941 aaccgaaatt gtcgtaattc tgacgttttt cagcgtcgct taagacggcg taggcttcgc
    30001 tgattttctt gaatttttct tcagcggcgg ctttattgtt tggattttta tccggatgcc
    30061 acttgacggc cagctttcgg taggctttct tgatttcatc cgcagtagcc tttttatcga
    30121 cccccaaaac ctgataataa tcttcagcca taaaaatgtc ctttgttttt tatatttaaa
    30181 atatccttat ggcggtaatt taagcaggaa ttcccatttg aaaagcttca agattcaatt
    30241 ttacaagttt ttgcggaaaa gatttccgga tgacgctttc ccaaatcgag gtcttaatgt
    30301 ccattaacga agagagggtt ccgagaagga cgacattggc tgttttcagg ttgcctgctt
    30361 tcagcgcaag cgaaagcgca tcaacggcaa tcaccctcgg gtaattattt tttaaaaact
    30421 caataatatc atcaggatat tgagaatccc cgagattgac ggccgggggg ttgatttgtt
    30481 cggtattcag gatgatggaa gcgtccgcgc ttaagtattt caggtagcgg agcgcttcca
    30541 ttttctcaaa ggaaacaagg gtttcaatgg ttcccggctc tgccagcggg gaataaacct
    30601 ttgcgccgta gcgaacgtgg ctggtaacac agccgccgcg ctgcgccatg ccgtgaactt
    30661 cgcttttctt gacgtcgtaa cctgcttcca tcatgacttc gcacagaata tcgctggccc
    30721 gcaggatgcc ctgcccgccc acaccggcaa acaggatgct ttttaccttg tcgtctttca
    30781 taaacctttc ctctttttca gccaatcgcg ccgaatttgc agacctgggc gcacaagccg
    30841 cacccgttgc acagcgtctc gtcaatttca gcgattcctt ttcgcttgga gccctccttc
    30901 gtaacgcctt ttttccaggc gatcggcggg cagttcagtt ccagacaggc ccggcagcct
    30961 tcgcacgcat ccggatccac cttaagcggc ggtttgggct tttggtttgc ccttttaaac
    31021 aggacgcagg ggctttgggc gataatgacg gaaggaccgg agctggccag ttcgtccttg
    31081 atcgcaatgc gggcttcctt cacgttgttc ggatcgatca cccggatgtt ttcaatcccc
    31141 agggctgata ttaaaatatt caggttgact tttttggttt ctttccccat cagcgtgtag
    31201 cctgttcccg gatgctcctg catgccggtc atggctgtgg tgctgttgtc cagaataata
    31261 atcagggcat cgctttggtt gtaggccatg ttcatcaggg gggcgatgcc ggagtgaagg
    31321 aaggtggaat ctccaatgac gccgaccacc ttccctttcc ctttgtcctt cagggccttg
    31381 ctcatgccgt gggccatgcc gatgctggcg cccatgcaga tgcaggaatg cagaccttcc
    31441 agaggcttca gataggacag tgtgtagcag ccgatatcgc cgtgaacata cgtttttgat
    31501 tttttgagcg cgtaaaacaa tccccggtga ggacagccgg gacagaggtt cggcggccgt
    31561 tgtgccaggc ttcccggata gggcgaaacg gcgtcctgcc cgccggaggc aatggaattc
    31621 cggatgacgc cgggatcaaa ttcattcgtg taggggaaga tttctttgcc ggtcacctcg
    31681 atgcccatgg cccggatctg ctcttccaga tagggatcga gttcttctac aacataaatc
    31741 tttttgaccc tggaagcaaa atgccggatc attttcaccg gcagcggata gaccatgccc
    31801 agcttcaggt aggaataatt cgggaaaacg tccttggcgt aattgtaggg catgcccgcg
    31861 gtaatgatgc cgacttcggg gtcgttgatt tccattttat tttcaggaaa cgtttccgca
    31921 aatgaaagca gggctttctg gcgcttttcc acttccacgc ggcgggtgcg cgcgttgatg
    31981 ggaaccatga cgtattttgc cgcgtgatgc gccagggtgg ttttgtcttc gggaatccgc
    32041 ggttcaccca gcgtcaccac ggatttggaa tgggagacgc gcgtcgtcgt gcgcagaaag
    32101 accggcgtgt cgaattgttc gctgagggcg aaggcgagct tgacatactc cttcgcttcc
    32161 tggggatcgg ccggctccag catgggaatt ttggcgaact tggcgtagtt gcggttgtcc
    32221 tgctcgtttt gcgagctgtg catggacgga tcatcggcgc tgatgatgac cagagccccc
    32281 gcgattccgg tgtagctgac ggtaaaaatc ggatcggcgg ccacgttcac gccgacgtgc
    32341 ttcatcaccg ccagcgccct ggcgccgccc aaagccgcgc cgatggccac ttccaccgcg
    32401 actttttcat tgggcgccca ttcggcgtaa acgccgtcgt accgggcgaa ctgctccatg
    32461 atttcggtgc tgggcgtgcc cgggtaggcc gccgcaaacc tcacgcccgc ctcatacacg
    32521 ccgcgggcga tggcttcatt tcccgacatc agtacttttt tcaaatgttg cgcctccgtt
    32581 aatcaactgc tttctgaaac cggatgcgaa aaatcagccg agagcggcga tctttcgctg
    32641 aataaaaatt ctggccgccg gttccgttgt tttttccaga gcctttttat aatattccag
    32701 agccttcgct ttgtcgttca attcttcgca agcgcgcgcc aggttgcgca ggctcatcat
    32761 ctcgaagccg gtatgataaa ccttttgcgc ctgctcaaaa tatttcaggg ccgcccgaaa
    32821 gtcctttttg gcttcgtatg cgtatcccag agacgtcagc gccagaaact tgacccccgt
    32881 tttatccgat cccgttttgg cgatgaattt ttcgtaggaa gtgatcgccc tgtccatgtc
    32941 tcccttattg taataaatgt tggccagatg gtaatgggcc atccggccgc tccaggtgta
    33001 ggaatactga tccgtcagct tttgaaagac cggaatattg gcgtccgccg tttgcggatt
    33061 atcgccgctt ttcaggatat tttcctgagc ctgggcatat aatttcaggg cggcgccgga
    33121 gtagtagctc cagtaaaaat aaatgcccag aaccagcagc acggcgcagg ccagggctgt
    33181 tccggcaatg agcacacggc ttttattttc ctcaacgtac tcgcccactt tctcaaaggc
    33241 ggtttgaaaa gcgtccgggc ttttcagttc ttttttggat aacttggcgg ccattttact
    33301 cccctttgct aatggaatat ttttcaatca atccggtgat cagcgccggc attctgcgga
    33361 ttttgcccac ggaattcgtg caggcatgga tggtgtatcc ttcggcgtgc agcttctgcc
    33421 ggttttcgtc ccagattttg gagccaaacc ggatgctggc cctcttgatg tagtcaaagc
    33481 tcgtttccac ggtgagcagg tcgtcgtagg ttgccggcag gaggtagcgg cagaatacct
    33541 gcgtcagagg cagatggagg cccagttgat tcaaatccga ataaacaatt cccacctgcc
    33601 gcatcagttc gttgcggccg tgctcaaacc agcggatata gttggcgtga taaacaatgc
    33661 ccattgcgtc cgtgtcggcg taaatcacgc ggatttttgt cagattactt tccaagaaac
    33721 gcgctccagt cccggaggaa tttgtccatc agcagatgtc cgggagacac aacaagaccg
    33781 gcggcgccgg cctgggcttc ggaaaatttc aatccccggg cgagattttc ttcccgcctg
    33841 gacgacagaa cggcaaaagg gctcggatcg cccacatggg ttttcacgtc gatcggcgtc
    33901 ggatgatcgc tgagcaccag aatgcggtat tctccccgtt caccgatgcg cgtcagaatc
    33961 ggtccgacaa ttttttcgtc gaaaaactcg atggccagaa tcttcccttt tgcgttgcct
    34021 tcatggccca tctcgtcggg cgcttccagg tgaagaaaca caaaatccat gaacttcagc
    34081 gcgtcgagcg ccatcgcggc cttcccttcg tagttggtat cgatgtaccc ggtggcgccc
    34141 tccacgcgca gcggtttcaa accggcgtaa atgcccagtc cgttgagcag gtccaccgcg
    34201 gaaatcatgc cgcccctgag tccgtatttc tcagtcaaag gcgccatctg cggctttccc
    34261 ccctgccccc acagccagat ggaattggcc ggtttcttcc catccgcgac gcgggcggcg
    34321 ttgaccggat ggtctttgag gatgtccgcg gcggcgcgca tcagtccgag aatttcctcc
    34381 gcgcaatcgc cctgcggcaa aaacgggaca atgggcttgc cggggatgtc gtgcggcgga
    34441 gtggtttgcg gagacccggc cgcgttgcgc cacaccagca gatgacgata cccgacgccg
    34501 ggataaaact gataccggga cgtgttcagc tttttgttga ggtccaggat gatttccctc
    34561 gcctcgggcg aagaaatgtg cccggccgtg aagtcgtcca tgaacgccgt cccctcctgg
    34621 ggctctccga tatggaccag gttgcagcgg taggcgatgt cgccttgccc gagcgcaatg
    34681 ccctggcttg cggcctccag cggtccgcgg cccgtgtagg tttctttcgg gtcgtagccc
    34741 agcacgctta aattggcaac gtccgaaccg ggcgtaaagc cttcgggaat ggtatccacc
    34801 agccccaggg ttccttccga ggcgatctga tcgagaaagg gcgtcacggc gcactgaagc
    34861 ggggttttcc ctccaagctg ccggcttgga taatccgcca tcccgtctcc cagcaaaacc
    34921 acgtatttca cagatccacg cccccttacg tccttgttcc cgcgcctgcc gccgtcgcct
    34981 gcgcccggct tacaactcat cctcgatgcg aataagaatc gttttatccc ttacaatgtc
    35041 gagccggtcg atttttttca acgcctggcg aacatttttc tctttcgctt tataggtcgt
    35101 catcacgatg ggcacggcgc ccgcctcttc cctcgccttc tggatcacgg tggccaggct
    35161 gatgtcatgt gcgcccagaa tgccggatat ttttgacagg acgcccggac ggtccagggc
    35221 ggtgaaccgg aagtaatact tcgtctcgat gttgtccatc gggaccagcc ggatatcctt
    35281 catgccggtt tcatcaatgg accgcagcgg cacccggccg gaaatcccct tcagtacatt
    35341 gcgggcgctg tccagaatat cgctgaaaac cgcgctggcc gtcggcatca tgcccgcgcc
    35401 ctggccgaaa agaaagaccg gtccggaggc gtctcccacc aggtggaacg cgttgtagtt
    35461 gtgattgacg ttcgccagca gatgccccga cgcgatcatg gtcggatgga ttctggcctc
    35521 caccgcatcg cctttcagcc tgccgattgc cagcagcttg atccggtaac ccagctcccc
    35581 ggcgaaagca atgtcctccg cgctgatttt cgtgatgcct tccaggtaaa tatccttgag
    35641 ccggactttt ttcccgtagg cgagcgacag gacaatggcc atcttgtgtg acgtgtcgat
    35701 gccttcaata tcaaacgtcg gatcggcttc ggcaaacccc agctgctggg cttccttcag
    35761 gaccacatcg aagggcttcc cctcattcgt cattttggtc agaatgaaat tgcaggttcc
    35821 gttcataatg ccgactatgg agtggatccg gttggccacc agagattcct tcagtgtttt
    35881 gatgatcgga atggtcccgc cgacactggc ttcaaaaccg atatccacat tttttttctg
    35941 cgctgccggg aaaatctcat tgccgaacgt ggccagcatc gccttgttgg cggtcaccac
    36001 atgcttccct tttttgatgg cttccagaac aaaggtacga gccggttcat agccgcccat
    36061 cagttcgatc acaatggaga tgtccggatc gttcagaatg tcgcgggcgt cggtcgtgag
    36121 cagttttttc gggattttga cgccgcgcgg ggaggtgatg tcgatatcgg cgattttctt
    36181 caaaacaagc ttcgcgccca gtttctgggc gatcaatttt tcattttgct caagcagcct
    36241 gacgacgccg gttccgatat tgcccaggcc gatcaggccg atggatatgt ttttcatttt
    36301 ttacctcgaa atcaataaga aatccggctt cgcgccgctt ctccccgcct gttttcgcaa
    36361 gggaggtacc ctatatcaaa tcccgttgtt ttgtcaaatg gataagcgat cgaggccctg
    36421 tccggaccga agaggtcacg gggttaattg acggcaaacc tcatcgtgaa gaggcgtggg
    36481 aattcccgcg tcccggcccg cccggcacag aaacgacgtc agggtgtcca cttccgtctg
    36541 tttgcccttt tccctgtcca gttgcatgga ggttttcgcg tcatggctga agccgccgat
    36601 catttccatg gttttgtcca ctgcgttttg gggcaggcga atccggcgcg ctttggcaac
    36661 tgcggcgact tccatcatca tcgcccgcag acgttcgcgt aaactttcgt cctcgcggat
    36721 gaagccgtat gttttgccgg ttgcggccgt gagcgatccc agggggcaca tcatcaggaa
    36781 tttcatccac agcgcttccg ttattttgtc cgtcagccgg gcgtcaattt ttgccgcgag
    36841 cagaagatcg agaatgtttt tgtaccggtc ggcggatgcc cggttgtccg ttccgaaaat
    36901 caaccggcag gcgccctgcg tctggatcac tacgccgggt tgatccagga aactgatgat
    36961 gtaaacgcag ccgctcgcga tgtcggcccc gggcagcacg gcgcgcagcc tctctttgct
    37021 gtccacgccg ttttgcagcg ggatcactgt cgttttcctg ctgatgttcg gcgccagcga
    37081 gcgcgccgat tcctcaagcg agtagctttt gacgcagagc aaaaccagat cgagcgggcc
    37141 cgccagggac gggtcgtctg tggcaatgtt cggccaggcc gtatattggc cttcacgggt
    37201 gtaaagctga aggccgtttt tctgtatggc ccggagatgc tctccacggg cgataaagat
    37261 aatttcatgc tttccggaag aggcatattc ccgggccagt ttgccgccga aatatccccc
    37321 taccccgccg atgccgacca ctgcgattct catcgaaaca atctcctttt ccgtcgcgag
    37381 ttaaacgaaa tcctccaaaa gatcaagtct caatataggg gcttcagtag ttgatggaca
    37441 actggactcc gatcagctgc cgggcccatt cccatgagtg atcaacattg gaaaaatcca
    37501 tccggtaata ggggccggcg gaaatatgac ggctgacggc cgcgttgatg ccgattttca
    37561 ggcgattcag catcaaggct ttctcccggt tttcgtagta ggattccagg ccggcgtagg
    37621 gagtcagaag agccgcggga agcgaaatca ccagaatgtt tttgagtctg aaatcgttcc
    37681 actccggcaa aatccggtat tcgaaaagga tttgttcctc gatggagaaa ggaccgatct
    37741 ttgtccgcag gttcaggctg gcgcccggat cctgctccag cgcccaggaa cggccctcgt
    37801 ctttcgtgta cccctggcag tactgaatct ggaaggagag ccagggaagc ggcagctgat
    37861 agttcaggcc gattccccac tccgcatagt catagttcga catttcatcg tcggcctgga
    37921 tgtaggcgta ggagatgccc cggaattttt cattgaacgt gtaggaaaaa ttcaaatccg
    37981 tccgcagcgc cgtgtcgtgg tccgcataca gacacggcgg gcatccgcag gccaggatca
    38041 gccctgataa aaccagcagg aaatgccgca aaaaactgtt tttgaggtgc atggaaagta
    38101 tcttcaatcg gtttctttca aagacctgtt gcccagaaga cactttgagc ggccattggg
    38161 agcgttttgt ataataaatc ttggcgaagc gaaagatcag cctttgacaa ctgtttgagc
    38221 cgaaggcgag ttttgtcatt gcctttcgca agccttagat ttatacaaaa cgctcacagg
    38281 caagcgaaaa gggtatctgg gcaacagccc gtccgcaccc tttatttcgg ggcgcagaga
    38341 aaaccgcctt tcggaaaggc cttgccattc tcatcctggg ccttggggtg ataaccgacg
    38401 gccttcttct ccgcgccgca cacctgtttg ccgaggtttc ttttcgattt cggcaccggg
    38461 ccgaaagcct ccgggcggtc ccacagcatt tcattgccga gaatcgcgga ctggagcttg
    38521 ggcgggacgc ggtccggttt cttgcccggg cccgcggcaa acgctgccat ggaaaccgcc
    38581 aaaaatgccg ccaccgtcaa cactgcgatc attcttttca ttttcatact gtgctccttt
    38641 cggttgaaaa ggttgttaaa aattcagagg cgttccctct cctttttgct tttttacacc
    38701 cgtaccgggt aaaatgatat catcccgttc tggccagatc cagctttccc tgccatttcg
    38761 cgatcagcgt ttctttcaaa atggcatgtt cgggtttggt caggagcgga tcgcgcgccg
    38821 ccagtgcgaa ggcgtcctcc ttggcgtcgt tgagaatgcg ggcgtcacgg ataatgctgg
    38881 caatgcggaa atcgggcatc ccggactgcc gcgttcccag aaaatccccc gggccgcgaa
    38941 tggccaggtc ttcgtcggcc aggacaaaac catcggtggt tttctccatg accttcagcc
    39001 gtttgcgcgc gtcgcccgac gccttgtagt cggccagcag gatgcaggcg gagggaatat
    39061 cacggcggcc cacgcggccg cgcagctgat gaagctgcga cagaccgaag cgctccgcgt
    39121 gttcaatcac catcagcgag gcgcgcggca cgtcgattcc cacctcgatt acggttgtgg
    39181 aaacgaggat cgcgattcga ttttccagaa aatccgtcat caccgcgtct ttttcccggt
    39241 ccttcatctt gccgtggatc aggccgactc ggatatccgg aaaaacgtcc tgctgaagat
    39301 gttcggccat ttttgtcgcg tctttcaaat ccagattctc cgactgctcc accagaggat
    39361 aaacgatgaa cacctgatga tgatttgcca gctctttgcg aatggcctca tataccgctt
    39421 cccttctgtt ttcccccatg accaccgtgc gcaccggttt tttgccgggc ggcatttcat
    39481 cgatcaccga tacatccaga tcgccgtaaa ccgtcatggc cagcgttcgc gggatgggtg
    39541 tggcggtcat caccagcaca tccggggaaa ggcctttgtt gcgcagcgtt gcccgctgca
    39601 aaacgccgaa gcggtgttgc tcgtcgatca cgaccagccc cagcttcctg aagtccacgt
    39661 cctcctggat gagggcgtgc gttccgacga tgatgtcggc tttgccggac cggatgcgct
    39721 ccagcgcctc tgtgcgtgcc gcgccgttca tgccgccggt cagcaaaacc acgcgcagtc
    39781 cgagcggttc ggcccacgca cagagtgtcg catcgtgctg cttggccagg atttccgtag
    39841 gcgccatcag cgcggcctga tacccgtttt cgcacacggt gatcatggcg gacatggcca
    39901 ccagcgtttt cccgctcccc acatccccct gcagaaggcg gttcatggcc gttggcttct
    39961 gcagatcctt ttgaatttct tccaccaccc gtttttgcgc gccggtcagc tcaaacggaa
    40021 gcgaggcaaa aaattttgca agccaattcc ctccgcggga gaaggaaatg cccttctcca
    40081 gaacccttcc ggatttcctg acggccattc ccagttggaa aaagaaaaac tcgtcgtaaa
    40141 tcaggcgccg gtgcgcctcg gatcgcgcgt cgagaaaagg ggccatctcc gcgtcgcttc
    40201 cgggaaagtg gacgctcacg atcgcctcgc gaatattgat caggcttcgc ttccggcaga
    40261 tgtccgcggg aatcggcgag gcgatatagc gcgaataatt ttccagcgcg gaggccatga
    40321 ctttccggat gtatttctgg tgcaggcctt ccgtctccga atagaccgga acgatgcgct
    40381 tgaagtggag caggttttcc tcatcctcct cgtccagaat ttcataatcc ggatgaatca
    40441 tggatttgcc cgtgtagccg ggcgtcacgt tgccggtaaa aatcacccgc gtcccctttt
    40501 taaaagcgcc ggccaggtgc gctatccggc ctttgaacca tttggccgtc agaatcccgg
    40561 tatcgtcgct cacggcgact tccagaatcc ttcttttccc gtaatagcga aaggcggcct
    40621 ccgaaacggc ggccacgacc gtctgggtcc tattcgtctc cagtttgaaa attttacgga
    40681 tttcccggcg gtcttcatac gtccggggca gaaagtagag taaatcttcg accgtgaaaa
    40741 ttttttttgc ggcaaagcgc aatgccattt tcggcccgac gcccttcaaa aactgaaccg
    40801 gcgcggacag cttttcacag gacgttttca ggtcgttaat ccgggcggca atctgttctt
    40861 cccggagagg agcggcagga tggcgcgcgt caaagttttc ggcggcgtgc cggagccttt
    40921 ccagaatcac caaggcttcg gcaatgctgc ttcttttacg gtcctcgtct ttttcgtcgt
    40981 actccagaaa aattcgcatc atgtggtcca gggcggggcc gaaaaggttt ttcgcggcgg
    41041 aaggaatggc gtcaatctgc aaagcgatca aaccggacag agacttcccc agattcctga
    41101 tgttcgggag atttttaaaa tgatccttcg cggcaaaggc gagcggctgc tcgatgcgct
    41161 gcaggttctt tttaaaagac tccatttctt ccctctccgg aaacggaaaa tacaggaaat
    41221 gctttgttaa ttttttaaca aataaatttt gtttgcgcaa gtcaaggatt gacacgcagg
    41281 cggatttacg ttaagtcgtt tccgtcattt atcacttgaa aaataaaccg gtatttcatg
    41341 gagccctatg ccaaataaat ccgtaaaatc aaaaccttct acatataaag aatcaggggt
    41401 ggacatcgag aaggccaatg cctttgtcga gcgcatcaag ccgctgatca aaacgacggc
    41461 ccgcaaggaa gtgctctccg gaatcggcgg tttcggcggt ttgttccgct ttgacgccgc
    41521 caagaccaaa aaccccattt tggtttcctc caccgacggc gtgggaacca agctcaaaat
    41581 cgctcaactc atggacaagc atgacacggt cggcatcgat ctggtggcca tgtcggtcaa
    41641 tgatgtggtc gttcagggag cggagcctct gttctttctg gattacctgg ccaccggcaa
    41701 aatcgaaccg gaaaaaagcg ttgcgatcgt ggaaggcatc gtccagggct gcaaacaggc
    41761 cggctgtgcg cttttgggcg gggaaacggc tgaaatgccg ggattctacc cgcccggcga
    41821 atatgatctg gccggattct gcgtgggcgt ggtggaatcg gaaaagctca tcgacggctc
    41881 cacgatcagc atcaacgacc gggtgatcgg gctggcttcc agcggtctgc acagcaacgg
    41941 cttttcactg gcccgcaagg tgcttctgga aaaaggcagg ctttccgtga aggataccgt
    42001 gtcggggctg gcgcgaaccg taggcgagga gctgcttgag ccgacgcgga tttacgtgaa
    42061 accccttttg aatattttca agagctttcc ggttaaggga ctggtgcatg tcaccggcgg
    42121 cgggttctac gacaatatcc cgcgcgtcgt tcccgatgtc tgccgctgcg tgatccggcg
    42181 cgggagctgg gagattccgc cggtcttttc cgtcatccgg gacatcggcc atgttgagga
    42241 gcaggagatg ttccgcgttt tcaatatggg catcgggatg atcatggtcg tgccggaaaa
    42301 ggacgcgcag gaaattctcg accgtctcaa gggactgggg gaaaacgcct gtctgctcgg
    42361 tgcaatcgaa aagagggacg aaaacgggcc tccggtctgt tttacggatc tgtaaggcgg
    42421 ttctatggcg gattttttaa agctgggcgt cctgatttcc ggaagcggct ccaaccttca
    42481 gtccattatc gatcagattg aacggggagc gcttaacgcc cgaatcgcca tggtcgtcag
    42541 caacaatccc gaagcttatg gtctgacccg ggcgcaaaag catggaattc ccagcgcggt
    42601 tttgaaccat caggattttg aaaaccgcga ggattttgac cgcgaactgc tccgcgtgct
    42661 cacggacgca ggcgttgaac tggtggcgct ggccggattc atgcggattc tctcgccgtt
    42721 tttcctgcga gcctttccgg accggatcat caacattcac cccgccctgc tccccgcatt
    42781 ccccggaacg cacgttcagc agaaggcgct ggattacggc gtccggtttt cgggctgcac
    42841 ggttcatttt gttgatgagg gcgtggatac ggggccgatc attattcagg cggtcgtgcc
    42901 cgtttgggcc ggggatacgg cgcagacgct ggcggaccgc atcctgaagg aagagcataa
    42961 gatttatccg caggccatcc gttatttcgc tgacggccgc attgtcaaaa acggtcgccg
    43021 cgttgagatc aaagacagca gaaatcatga cgccgtgctc tgcaatccgc ccctggaccg
    43081 ctgaagggca gcttcccgcc gtcttggttt tggaggaaaa ttcgtgtatg atctgctggt
    43141 gacaggcgat gatctcgcct cccacgttgc cgccgcctac gcaagccgcc agggcctgaa
    43201 gacgcttttg gttgcccaga gcggactcgg agggctgcag atcatcggcg actttgtttt
    43261 caatctggat tccgcgccga tgaccggtct ggcttccggt cagcccggct tttccgtgct
    43321 tgccgaactg ggcgttgaaa ttcccgaaac gcatccgacc gccgtcagcc cggcgtatca
    43381 ggtgcttctt cccggccacc ggattgattt ctcttcagat cccgactcgc ttctctccga
    43441 attggcctgg gaatttccgg accgggaggc ggaactccgc gatttctacg gaagcgcaag
    43501 ggaggcttct tccgtttttc aggactggat tgccgaacat ccccggatcc agccgcaaac
    43561 cctgaaggaa tacgtctcct acctgaaaat tttccccttc ctgctgcagt acaaattcgc
    43621 cgccgcccgg tttgacagaa tgctgtccca agacgcgccg ctggaaaaag tgtgggaagc
    43681 gcagcaggcg ttgctttcgc tcaacacgga cgatttgttt tcctttgcct ccgcttttca
    43741 atacagcgcg ccgctgcgcg gcgtcgccta tttcccgcag ggaaagcagt ttttgttcaa
    43801 tgcgatgatc cgaaccctgg aagaaggcgg cggtctgtat ctgagcgggc attccatcac
    43861 gtccgttgcc aggcacaata cgatcacgct ggaggtgaaa gctccggacg gctccctgtt
    43921 tcaagccacc ggccgtcatt ggattgtcag cacgaaatcg gatgcattgt ccctcttgga
    43981 ggcaaaccgc agccacgtca ttttttcgga gagactccgt ccggcaagga ttgtcggcta
    44041 tccgttcact ctttttctgg gggttgccga aaaatctctg cccgaacaaa tggcccggca
    44101 cattgccgtt gtcacggatg tccataaaga tctctacgac cataacgtaa ttctcctgga
    44161 aacgggtctg cccgaaaaag aaaagcccct gttccaggca aaaaatttcg tgacggccac
    44221 ggtttacctg cctgccgaag acgacgtctg gaccggagag gccctgcggc aggaagccgc
    44281 atccatcatg gagcggctcg acggtttttt ccccttcttg aaagacggca tcgagcttta
    44341 cgatcttgac aaatccatcg aactttcgtt gaattaccga aaagttctct cgccgaaata
    44401 caaggtgcgc aatgcttttt ttacgtcttt tgcggccaag agcgatcgga caagattgaa
    44461 gaacgtattt ttaaccgggg cgtcgctgct gacggacgcc ggttttgacg cggagttgct
    44521 ctccgggaaa aacgcggcca tgcaagtcat cgaggaaagg aaattttccg atggaacatg
    44581 atttctcaat cgcctgtctg ggcccctgta cgattgactc tccgatcaac atcagcggat
    44641 ttgtgtccga cgaaaaccgg atattattca acacgcgcct ggacgattac gacgccatga
    44701 aagacgccgc cggaaagcct ctgtctctgg agcctgcggg cccccggaaa aagatttttt
    44761 tcgatcccgc aaagaccaag gcggccattg ttacgtgcgg aggcctctgc cccggcatca
    44821 acgacgtcat ccgttcggtg acgatgacgc tgttctaccg ctacggcgta aaaaatgttc
    44881 tgggaatcaa atacggactg cgcggattga atccggaata cggcaagata ccgatccagc
    44941 tcacgcccga ctacgtcaag gacatcacgc atatcggcgg cagcatcctg tcctcgtcgc
    45001 gcgggccgca ggatgtgaag gtcatggtgg actacctgga gcttctgaaa atcgacatcc
    45061 tcttttgcgt gggcggcgac ggcaccatgc gggccgcgga aaaaatcgcg acggaaatcc
    45121 attcccggaa aaaacagatc gctgtcatcg gcatccccaa gaccatcgac aacgatctta
    45181 acctgatcga aaaatctttc ggctttgaca ccgccatcgc caaaaccgtg gaagccgtcg
    45241 gcagcgcgca tgtggaggcc aagggcgcct acaacggcat cggtctggtc aaaatcatgg
    45301 gaagattgtc cggctacatt accgccacgg cggctctcgc ccagggagac gccaactttg
    45361 tgctgattcc cgaagtgccc ttcgaactcg acggcgagaa gggattcctc aaaactctgg
    45421 aaaaccgact gaaagccagc ggacacgcgg tgattctggt ggcggaaggc gcgggtcagg
    45481 aattgatgaa gcaggacggg caagagaaaa aaacggaccc ctccggcaat gtccggctcc
    45541 acgatatcgg gctgtttctg aaaaacgcca tcgatgtcta tttccaccgc atcaacatgg
    45601 aaatcaactt gaaatacatc gaacccagtt acctgatccg ttccgtgccg gccaactcct
    45661 cggacagtct gtactgcagt tcccttgggc agtactccgt tcacgccgcc atggcaggga
    45721 aaaccggcct gctcgtagcg ttgagaaagg atgaatacgt ccatctgccg cttagcgccg
    45781 ccatttccgg aaggcagatc gatccggacg gcaacctctg gtttcgggtg ctggaggcaa
    45841 ccgggcagcc cgcggaaatg aaaaaataag tttcatagac tgaaggccaa agactgaagg
    45901 cggtgaggaa taaaaaataa cttgcaaaat aaagcattct ggcttannnn nnnnnnnnnn
    45961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46021 nnnnnnnnnn nnnnnnnnnn nnnnnngact gaaggcggtg aggaataaaa aataacttgc
    46081 aaaataaagc attctggctt agtaacgacg cactttttta aaggagtttg atcatgtctc
    46141 ggatttgcga agtatgcgga aagaaaccgg ccatcggcaa caacgtcagc cacgccaaca
    46201 acaagacccg tgtgatctgg cgtccgaatt tgcagaaatt acgctgtgtt gacgagaaaa
    46261 caggatccgt gaagcgggtc aaagtctgca cgcgctgcat ccgctccggg tttgtgaaaa
    46321 aagcaagcta gcgcgtcgga agccacccgc agtttcggat ggtcccgcaa gcctcctgga
    46381 gaaaattctc atctccaatc gaaagcaagg catcctgaaa cagcgctgaa atcgtttctg
    46441 taaacaattc attgttattc cgaaggccga atttgtccgt aaaaaggcga atccggcctt
    46501 cgatattcat gaaccgctgc atcctttctc tggtgatgta ggatacggcg atcttgagaa
    46561 cctcgcggct gtgttcggga tcaataccgt aaagccagga tatatctgag caaaattcct
    46621 tttctctccg gtcgaacgtg aaatgaacgg cttcttccag aaggtcgatg gacccgctcc
    46681 ggtcgatgcg ttgccggatc acaaggtcat aaatcatttc caccagtaaa tgggactggt
    46741 aggccgcatc gctgaaggat atttccttgt cgatcattct gtgcaaattc ataatgtggc
    46801 cgaccagatt gcgccccttc aaatacgtat agcccgatga gttcgggtca aagccttcct
    46861 gacaggtcat cgagatgctg ccgtaatgcg ccagatcatc caccagcaga tgaaacatga
    46921 cgtaggccgt gcgctgatgg tcgggcggcg cctcgatgaa ccgggggata aaatgggttt
    46981 gccgggggct gatatcggga tgaatcgtca ataaatccgg agcgatattg taaatgtaaa
    47041 tgtccggatc catgtcgtgc attccggagg cgctcatcat ttgctgcaac aaacacgtat
    47101 gggttaacat caacatggaa atgcctgacc gccgccgaat ttacttcatg cgccgattag
    47161 catttattgt tttaaagatc aaggtggtcc ggcttatgat tgttcaggca aaaaacgtaa
    47221 atcaaaataa cggtggactt ttcatggaag cttcgacggt tgaataagat cttccatatc
    47281 gcaattcaag gcctttgcca gcttcatcaa taaatcgacg ctgggtgttc tctttccatt
    47341 ttcaatttga gaaatggctg ccacggaaac accgcaaatt ttagccagat ccacgagcgt
    47401 cagtttcctg tattcgcgcc agaccttcaa gcggcttttc ccgaaacaca aacgttttac
    47461 aaaatcttca ccataggttt cttctccttc ggcaatggcc gcccttacaa ctcgcgcatc
    47521 gcgaatatct tcaagctctt ccagacggct gatgattttt tcccattctt caaacggaac
    47581 aatggcgtat tccggtgcgc ctttatgttt aatgatttgc acgttcattt ataaacatct
    47641 cctctcgatc ctattttcag gacatagaca atcaattctt ttttgacaac gtcacagatc
    47701 acccgccagt tgcccactcg tagcctccaa aatggtgagc ctttcaacct tttccagtct
    47761 cccttgtatc tacttggttc ctttgcgatt ttttcaagct ccgcatgaat cctctgtgca
    47821 accacttcag gcatctttaa gaatgtttta acggcctgct tgctgtacct tatagtaaac
    47881 atgtttatat gttaactcat tgttaaatat tggtcaacgt tattttaaca tttcttcctg
    47941 tattagtggc tttttacttc agccacgcca tcacttccgc tgcactcccc agcatcacag
    48001 gattctgctc gttgccggaa attctgcggg acatatacgg cacgttctct ttgagaaacg
    48061 cgtccatctc gcccacggtg atcttccggt cacggttggc atccgcctca ccctgcagcc
    48121 ctttgaaaaa ataatacgtg tagagcgagt ggcgtttttc gggataccag gaagacacct
    48181 gatccacggc ggcgctggtt atcaacacgg cgtttttcgg tccgcgatat tcgctcttca
    48241 ctttcagaat gccggggctc acgttcttga aaagcatgcc gcggtcggaa ttgccggaaa
    48301 aacacgtatc caggaccacg gtaatctttt tggccggaat tttggagaga ttgtcgtaaa
    48361 aggtttgcag gcgatagccg ttggttttca aatcctgagg attggcatcg accggcacaa
    48421 agtaggcttc ctgcgtttcc agatcgggcg cgccgtgccc ggtataatag acaaacactt
    48481 tcgacttgcc ttccttcaca agacggaaaa gcctgccctt gtggtttctt tcattgccga
    48541 acaattcata aaatttcccg accgtcgcgt cttccgcgta taggatattg gccgggtcat
    48601 agcccatcgt tctgaccaga tattccttca ttacctgcgc gtcacggacg gcaaattcca
    48661 cctccggcaa tcccgaggct gaatagttgc ggttgccgat caccaccgcc gcatcgaatt
    48721 tccccgcctt ctgcccgtcg ggaatattca tatccacatc cacggaaaga ccgcccgcca
    48781 gcgagatctc ctgctttggc gctgtgtctt cgcctttgac cacaaattcc tgcgcgcggc
    48841 gctgcggcgc gttcatcgcc agcgccgggt ctttttgcgc gttgaacttc gggcgcgcct
    48901 ccgtcaggtt cacgacgatg ggaatttttt caccgccggc aatgcggttg ttggtataga
    48961 acatgaattt gaaatcccgg tatttgccgg tcggaatcgc gcccaggtta aaatgggtct
    49021 gggcgtcgcc cgccagaaag acgttttgcc ccacctgcac atccgccgcc acgccgcgcg
    49081 cgtcgccgtg tcccatgttc tgcacgcgca ccgtcatttc cacaatctcc atcggctcca
    49141 cgcggctgtt gccgttctgg tcgttgacgc gcatatcggc gatgaccagc ctgggcggct
    49201 cgaaggcttt cgttttggca gaacaacatc agaatcattg agaaatgctt gagtatggaa
    49261 aacatgatga tccctccgga gcgggaagtg tgagcaatgt aataactttc gataatcatt
    49321 tcaagcctat ttcatgcaaa ccaatgaaaa acttgacata tgaacgatcg ctgatatgat
    49381 tacgccgaaa ataaaagagt aaagaagaac acatgaatga acggggacaa ctgccggtaa
    49441 cgcaatggaa aataaaggat tccggcccta aaaacataga agaatcgctg gtgaaggaat
    49501 tcggcgtcca cccgattgtt tcccagttga tcctaaatcg ccacgtggac agcctggaag
    49561 gcgcgcaacg ctacctgcac ccttccctca acgacctgca cagccccttt ctgatgcagg
    49621 acatgagaaa aggcgtggcc cgtctgctga aggcgatcta cgacgaagaa gacatcgtca
    49681 tttacggcga ctacgacgcg gacggcatca cctccgttgt cattttgtac aaatttatca
    49741 aacagttgac cggccgcgtc tcctattaca ttccggaccg ggttcaggaa ggctacgggc
    49801 tgaagattcc ggtcatcgat cagttcaaac aaaagaatgt aaagctgatt gtcaccgtgg
    49861 attgcggcat ctccgatatc gaacagatcg cctacgccaa atccgcgggc atcgacacga
    49921 tcgtgctgga ccaccacgaa atcgccgatc agctcccccc tgccgtcgcc tgcatcaatc
    49981 ccaaccgcaa ggactgcgcc tttcctttca agcgcctggc cggcgtgggc atcgccttta
    50041 attttctgat cgcgcttcgc ggaacgttgc gcaaagaagg tttttggaaa aacgccgaat
    50101 atcccaatct cagggaactc cttgacgttg tcgccctggg aacgatcggt gacatcgcgc
    50161 cgcttctggg ggaaaaccgg atttttgccc ggatcggcct ggatctgatg acggaaggca
    50221 gacgacccgg aatcaaagcc ctcaaggaag tcagcggcat tgacggtcaa ctgattgact
    50281 ccttcaaggc ttctttctgc ctgattcccc gcatcaacgc cgcaggcaga atcgcttcgc
    50341 cgctggacgc cgtcgaactg ctgctggcgg agacgctgga agaggcccgg ccgctcgcgg
    50401 aaaaactgga tctgcacaac cgccggcgtc aggccatgga aaaagacatc ctcaacgaaa
    50461 tcctggagca gctcggcgcg aagcccgatc tggaaaacat caacgccctg gtcttttctt
    50521 ccgaaaaatg gcaccccggc gtcgtgggca ttgtggcctc gcgcctggtg gatctcttca
    50581 gccgtccgac ctttgtcatc agcctcaagg acggtgttgg aaaaggctcc ggccgaagcg
    50641 tttcggattt caatatccac aagggcctgc agcagtgcgc gccgctgctt ctttcttacg
    50701 gcggccacta ccgcgcggcg ggcatctcca ttaaggaaga tgacatcgat gaatttgcct
    50761 gcatgctgga tgagatcatc cgcaactcgg tgcagtcctc ggaaatggtt tctcagactc
    50821 tgattgacgc ggagtgccgc ctgcaggaca tcagcctgga tctgatccgg cagatgtcga
    50881 tgctcgcgcc tttcggcagt gaaaatccgg aaccggtgct gtgctccagg aacatccggg
    50941 cgtcgtctcc cgtcattgtc ggcaacaacc acctcaaaat gcgcttggtt gacgacagcg
    51001 cgtccctcaa cgccatctgg ttcggcatgg gcaagcatct cggcgcgatc aactcggcca
    51061 acctcgatgt ggtgttttct ccgcagatca attactggaa cggttcattc gatgttcaac
    51121 tgaaaatcag ggacgcggcc attgtcgcct gaggagcctc catgaatcaa gagaccagag
    51181 cagccctgtt cagctttctt ctttttccgg gcggcgggca gatttttctg aagcgatacg
    51241 cgagaggaat ggcttttctg gcgccagcgg cagccgcgac acttgcgctg gcctggatga
    51301 tttttcaggt cgttcttgtt atcgtccggg aggcgctctc gggagcctcg gggatggacg
    51361 gcgtccttcc gggcatgagg aacgggctcg gggcgatcaa catgaacctc gctctgtggc
    51421 tgctgatttt gattttgttt ttgtggattt cttcgacgat cgacgcttat cgcctcggca
    51481 aaaaagcaga gccgcctacc acgtccgcaa atcgataatc atcttctggt ccggtttgat
    51541 tccgcccttt tccacaactt caaggacatc gattttgacg gtgcagattt ccttaaatat
    51601 cttcttgaag ttattctccc aaccatcaga atttacttga gaatttatat tctcgacccg
    51661 cagcgtcaaa acgtcttcat ggcctttgcg actgacgatg gcctggaggg gaaggccatc
    51721 gaatttggcg gaaagcagct tcatctgact gggagcgata aaaagaccgc ggacttttac
    51781 ggcgtctccc actctgcccg cgatgcccgc cagccggaag gatgtccggc cgcaggagca
    51841 tttctttgta atcagccggg ataaatcgcc tgtaccgaag cgcagaagaa agaaaatgtt
    51901 gttgaggcgt gtcacgacca cttcacccag ttcgcccggc gcgacatttt tccccgttgc
    51961 cgggtcaaca atttccacaa tcgtttcctc gcagatgtgc cagccggatt tcgcacagca
    52021 ttcgtaggcc acgtccccca cttcggtggc gccgtacatc tgaaaggttc tgattccgtg
    52081 ctctttttca aacctctgcc gcagcgaagg caggagcggc tccgcggcaa agcaggcctt
    52141 cttcacgcga aaatcttttt taaaatcaaa acccaactct ttggattggt cgatgatgga
    52201 cgcgaggaaa ctcggcgtgc ccacataggc cgtcacgttg aggtcccgga tgagctgaac
    52261 ctgaatctgc gacgaggatg tgccggaggg aaccaccgtc gcgcctactt tgcgcagacc
    52321 gttgtggaac gtgagacccg ccgccaccag atgataggaa aaggcgttga gcgcgatgtc
    52381 cttcggcccg atgccggccg cgaaaaacgc ccgtgcccag agatcatcgg tttcggataa
    52441 atgcggttcg tacaccggcc ccggagacgt gaaaatgcgc tcaatggcga cgtcagggtt
    52501 ttccagccct gcgtagggcg gcttctgcat ctccatttcc accagtttct cccgggaagt
    52561 taccggcaga atttccagat cctcacgggt ttttatccgg gcgggattga ttttgttcgc
    52621 gtccagcatt cggcggagcg cgggcgactt ctgatagccc agcctaacca tgcgcgccag
    52681 gagcctgtcc tgtcgggatt ttctttcttc gacaccccat gcttcctttt catcgtataa
    52741 ccgcttcata cccttccctc tgatggtcta atcatgcgtc cagtgtccaa gcgcctttgc
    52801 cgttcgccga accgtatcgt tgtttcgatt ttttttcagg atatccctcc ccgcactcga
    52861 tcttgcaagc gaaagacacg cctttcgcaa ttcattgacc agctttagac gatgagcgac
    52921 gacttcggga ctcatatcaa tcttcatgtt ccagactcct gaggtattca atatcatcca
    52981 aatcctgccc tgccttgcgc atggatttca gcagaatcag accctgtgga gaaaccactt
    53041 tcaacgggtg tcctttccaa tcaacggtaa gacgcgtctc ccacgcctga cgggtttccg
    53101 gagttgccaa gagcaaatcc acaataaaat attcccctga ttttttgtcc gccttgctca
    53161 gccggtgaat aataaccgcc ccctgattga atttcatcgg cgcagaactc atggtatagc
    53221 cgattttttg cagcactatc ttggttttcg aaagaaaatc cggaagaatc atcatatcaa
    53281 tatcgagcgt tgcccttggc tttgcataaa tggcaatggc caatccgccg cacaaggcgt
    53341 aatcaatgcc cgcttggtcc aatttcagag atattttttt aagctcgttt aagaggtcca
    53401 tgtttatccg ggttgcgccg aagctgaaaa agtcttttgt catctaccgt aattgacgag
    53461 gataatcaaa cgattattgc ggcaacagca ggcacagcgg gttacagcca gcgtttacgg
    53521 cggcggtagg atttgacgtc cttgtaggat ttggcgatgc ccgacgcggc catccccagg
    53581 taaaattcct tgacatccgg attgtcggac aattcctgag ccgtgccctc catgacgatt
    53641 ttgccgtttt ccatgacata accgtaatgg gcgacctgca gcgccatatt ggcgttctgc
    53701 tcgaccagca caatcgtcgt ggcgtgttct tcattgatct tcttgatgat gcggaagatc
    53761 tcctgaacca ccagcggcgc aagccccagc gaaggctcgt ccagcatcag caattgggga
    53821 tgggccatca gcgcccggcc gatgaccagc atttgcagtt cgccgccgct gcagtatccg
    53881 gccagcgctt tctttctttt taccagcgcg gggaagtaat gatagaccat ttccaggtct
    53941 tctttgtagg gttttcccgg ccgggtagct gttccgacgc gcaaattttc ttcgatggtc
    54001 aggtatttga aaggctggcg gccttccagc acctgaatga tgccctggcg cgtgatgaat
    54061 tcaggcgcct gattctgaat gttcacgccg ttgaagatga tgtcgccgtc ggtgacttcc
    54121 ccgttttccg gcttcaaaag gcccgatatc gccttgaggg tcgtggtctt tcccgcgccg
    54181 ttgctgccga gaagcgacac gatatgcccg cgcgcaaccg aaagcgacac gcctttgagc
    54241 acctgaatga cgttggaata aacgacggaa atattattca actgcaaaag aatctcgccc
    54301 ataaaatatc ctaaccccgc tgtacgggct acgtgcgtta acgaaagtat tttctgtaga
    54361 gaacacagaa aatacttgct aataggaaaa cggccagagc cggaaactgg agcgcacaat
    54421 gcgccaaagc tccgccagcc ctttcggttc cagtaaaatg aacagcacaa tcgccagtcc
    54481 gaaaacaaat tcgcgaagcg gcgccatgtt caggcccagg cccgcaatcc agccgacgtt
    54541 catcagagcg cccgtcgcga agcgcagcac ttcattgagc accgtgatga aggttgcgcc
    54601 gaaaatcgcg ccgggcatgc tgcccatgcc gccgatgatg accatggcga tgaattccac
    54661 ggaaagcgca agattgaagg gctccgtcgt gatgctgacc atgtagtagg cccagagggc
    54721 gcctgcgaaa cccgcgtaaa acgagctgat cgcaaacgac aggagcttgt accggaagat
    54781 cggaatgccc atgccttccg ccgcgcggtc gttgtcccgg atggcgacaa acgcgcggcc
    54841 gaatttgctg cgcatgatgt tgacggcgaa ccaggtcatg gccaccaggc aggcgaaaat
    54901 caaaaagaaa aaggccttgt ccccggcgat attgacgccc aggatgtttg gctcaggcag
    54961 tgtgatgccc atcgttccct tggtcaggct ttcccactgg atcaggacat actcgatgat
    55021 gaactgaccg gccagcgtgg cgatcgtcag atacagcccc ttgaggcggc cggagggaat
    55081 gccgaaaatc atgccgacca tggcggtgat cagtcccgcg gcggggatgc agacgaagaa
    55141 cggcaggttc aggcgcgtgg ccagaatcgc tcccgcatag gctcccacgc cgaaaaacgc
    55201 gccgtgcccc aaagagatct gcccggtgta gccgataaga atgttgaggc cgatcgcggc
    55261 aatcgccatg attccgatgt tgttcaaaat atacagcagg tagcggtcca ggatgaaagg
    55321 cgccaggccg aacagtaaaa tcaggaaaac gagcaggctg acccggccga accaggtgtc
    55381 aaaaacggcc agctcctccg catagctttc gcggaaattg ccgcagggat gaaaggacgg
    55441 ttttttcatt ttacactctt tcaatctcga ccagaccgaa caggccgtag ggcttcacca
    55501 tcaggatgaa aaccagcgcg atgtagggaa ccacgtctct cagcgacgga gacagatagc
    55561 cgccggtgaa ggtttccaga agaccgatga tgattccgcc gatgatcgcc ccgccgatgc
    55621 tgtccaggcc gcccaggatg acgacgggaa acactttcat gccgatggcg gacaggtcat
    55681 ggacgttcac gccgttgatg atgcccagaa cgatgccgct catgcaggcc accatcgcgg
    55741 caatcgccca ggacagcgcg aagacgcggc gaacatggac ccccagcgac agggccgctt
    55801 tctgattgtc cgccaccgaa cgcatgaaga tgccctgaga cgactttttg aaaaacaggc
    55861 cgaagagaac cagaaacgcc acgctgatca ccagcgtggc ggaatacacg ggcacaatgg
    55921 tgatcgaccc cagatgcaac tccagcgagg cgggcagaaa ctccggatag gtgtgcagat
    55981 tgccgcccca gacgagcaga atcaagcctt tgaacatggc ggcaagcccc accgtcagca
    56041 tgatgacaaa gatcagcttc tcgccgatga gcggccgtaa gaaaaacttt tcaatcagaa
    56101 aacccagcag aaagcatccg gccagcgtca gcacaaaggc ggccgcaacg ggaattttcc
    56161 cccaggtggc gagcgacaga aaaaagaagg cccccatcgc cagcagttcg ccgtgcgcaa
    56221 agttgagcac gctggatgcc ttgaaaatca tcacgaagcc catggcggaa atgccgtaca
    56281 gcagtccgac acagacgccg ttgaccagca attgcagaaa aaaatccata aaccgatccc
    56341 tcaatccaag tcgataatat taatggtggt ttcgatctcg ccgattcttc cgtcccggta
    56401 gcggattttc ccggtcaccg gcacgctttt cagtccgccg tacattgctt caatgatttt
    56461 gatgtacagg ccgtaaacga agcgccggcg cactttgccg gtgcgtgtca gttcgtcgtc
    56521 gtccgcgtcc agcagcttgt agagcagaac gaactttttg atcttcatta cgtcgggaag
    56581 ctgccggttc acctcctgaa cttccttcaa aatcagctct tccacggcct tctgctggga
    56641 cagatccatg taagtcgtat agggaatcat ccgctcttcc gcccagttgc cgacgttgcc
    56701 cagatcgatg ttgatcatgg cggtgatgta gggacgcgct tcgccgaagg taaccgcctc
    56761 cttgatgtag gggctgaact tgagccgggt ctcgatgaag tcgggcgaga aggcgttgcc
    56821 gctcttgttg cggataatgt cttccttgcg gccgataatc agcaggtggc cgtcgtcgtc
    56881 gatgtagccc gcgtcgccgg tcttcagcca gccgttttca aaggccgcct ccgtggactg
    56941 gtagtcgttg tagtatccgg aaaaattggc ctccgaccgg accagcactt cctgatcctc
    57001 ggccagtttg acgcttgtgc gcggcagcgg cctgccgacc gtttccagct tgacttcatc
    57061 gtccggctga atctgaaaaa tcccgcagga ttccgtgagt ccgtagcact gcttcagatt
    57121 cagaccgatc gcccggaaga atccgatgac gtcggggctg atcgggtgac cgccggtaaa
    57181 ggcgctgtga aaacgggagc agcccaggcg gtccaagagc gggttgtaaa cagccagttt
    57241 caggaaacca tacaacacat ggacgggcca ggagatgcgc tctttgcgca attttttttc
    57301 gacaaccgtt cggcccactc tctctgccag gtaaaaaagc ttccttttga gccagcccgc
    57361 atcggatatt ttcacgcgaa tgcgggacgc catgtcctcc cagaaacgcg aggccgtgat
    57421 gagcatggaa gggccgatat cccgaaaatc ttccaggacc gtctccggcg tttccggaaa
    57481 attcatcgtc atcgcgccgg cgagcgccac gcccatgccc cacatctgat cgacgatcca
    57541 ggcggtcggc gacatggaaa tccagttttc ttccggctga atgtccgcgg cttcaatcca
    57601 ctgaaatgcc atggcggcca gattgcgatg agacagcatg gcgattttcg gcacaccggt
    57661 ggtgccggag gtttgaatca tgacggcaat gtcctgcggc cttcccttgt cgatttcctc
    57721 ctctacaacc cccggatgat cggccaggta tttttcaccc cgctccagga gcgttgcgta
    57781 gctgatcagc cagggatcat tttcataaga cgtcatgccg gtgggatcga catacacgac
    57841 gttttgaaca tgcggcagct tgtctttgac ttccagaagc ttgtccgcct gctcctgatc
    57901 ctgcacgacg accagagggg agcggatgcg tccgatggag aaagcgagtt cctcggcgat
    57961 ggaggaagta aagagattca gcgatgtcgc gcccagcgcg tgcgcgccca gctcggaaaa
    58021 aagccattcg ggggaattgt tcacgaccat gcacagattg tctccccgcg tcatgttcag
    58081 ggaggcaaag cccgccgctg ttttcctgac ataatggaaa tagtcgttcc aggtgtattt
    58141 ctgccagatg ccgaaggctt tttcacggat ggccgtcttc gcgtcgccgt atttttcggc
    58201 gttggaagcc aaaagccttg gcagcgtgac ccggttttgt tttgttgaat gtgcggttga
    58261 cttgtccaaa gcgtttcgcc ctctttattc cagcgactgc gcgtcgccga gatacgcttt
    58321 aatcacctcg ggattggcgc tgatttcccc ggggctgccc tcccccagtt tctcgccgaa
    58381 gttcagcacc ataacgcgcc gggagatgct catgacgatg gacatgtcat gctccaccag
    58441 caccatcgtc aggccccagc ggccgttcag ttccagaaga aagcgcacca tatcctcttt
    58501 ttcttccaga ttcatccccg cgaacggttc atccagaatc aggagccgcg gctccagcgc
    58561 cagcgcccgc cccagttcca cgcgcttcat cattccgtac ggcagagagc cgacggtttg
    58621 cttgcggatg gactgcatct ccagaaagtc gatgatttcc tcgagaatcc ggcgatggcg
    58681 gatttcttct tcgcggacgg acggtgtaaa gaggcaggaa tgcaaaaagc tgtagccgca
    58741 gtgaatatgg cgcgccagcg tcatgttggc cagcaccgtc atgccgggaa acagttcgat
    58801 gttttgaaac gttcggccga tgccgatgcc gacgagctgg tgcgtgtgca gacgcgtaat
    58861 atcccgcccg ttgaaaagga tctgcccctg ctgggggcga tagaagccgg tgatgcagtt
    58921 gagcaggctg gttttgccgg cgccgttggg gccgatcacc gccatcagtt cgccggtgtg
    58981 gacggtcatg tccacgtctt ggaccgccac gacgccgccg aagctcaacc gcagattgcg
    59041 gatttccagt tcggctttct tatcctcctg gggccggttg atcattatcg aaggccggct
    59101 gcggaatgct ttcatgaaca cctcgacgcg ggtgttgacg ccgcgttttc ggcgcgggaa
    59161 gaccggatcc accggtcttc ccgcgccgaa agccttattt cttcagaact ttgattttgt
    59221 cttttcccgg aacgtagaaa tttgtcaagg gaatatacgt cccgttttcc atcaccttga
    59281 ccatgcgcgc ggtgaaggat ccctcatggt tgtctttcga gaagcttacc gccggaacca
    59341 gtccgccgaa atcttcattt ttcatggatt ccagcgcggc gttgatggtc ccgggattga
    59401 tcgccttcga tttttcgtag gcccgcagaa acgccctttc catgatcatg ccgaccacga
    59461 cgccctccca gtaggacgca tcaaaactcc tgaccgtttt gtagcgcttc cacaattcct
    59521 tcatcacgtc catgcctttg gatttatccg agggcagacc tccggggaac tgcatgacca
    59581 ggcggtcgcg aatcaacccc ttgcccatct tgaagaagtc cggatcggtg gaggtccagg
    59641 ttcccagaaa cagcggattg tatttaatgc gatccgcgct tttgaaggcg gtgatgatgg
    59701 ttgacggcag catctgaatg aaaatgtacc gggcgccttt tttctgcagg cggagcagct
    59761 ccgtgttgag atccacggtt ctgggcggaa attcctcgat ggccaccagg tcaacgccca
    59821 gctgtgccgc gtattccctg ctgggcgcat gaatcgatcg tccgtaggcg ttgttgtagg
    59881 tcaacaggcc gactttcggc gcttccttgc ccgtgtggat ggttttgata tattcgagga
    59941 ccgcccggca atccatttta taactgccga acggcaggta catgtagtcg atgggttttt
    60001 ccagaatttc ccagctggtg gaatagttga tcgtcggcac cttgtatttc tgaacaatgg
    60061 gcttcgcggc caggccttcg cccgcccccc acgtggcgat catatccact ttgtcctgca
    60121 gtacgaattt ttgaacggcg gcctgcgcct cgggcacctt gtaggcggtg tccacggtaa
    60181 tcatgtcaat tttatgaccg gccacgccgc ccttcacctc gttaacgtag cggaaataat
    60241 cccgctgccc tttttcatga aactgtcccc aactggacgc cggccccgtc aggttgatgg
    60301 ccgccccgac cttgatggtt tttgtctggg cgacggcctg ccccgccgtg gtcgcgagcg
    60361 ctaaaatgca aagcgcgagt aaaatcctgc cgcatgtttt catgatccct tccccctgtg
    60421 tttgatttta cgatgttcaa gtaaaaaaaa accgtgggca aattttcatc cgcccacggt
    60481 tcattcctct tcatgcgagg tcatgatacg tccaggcaga tcctgtcgcc gataaagcta
    60541 aaaaagaaaa aaccgactgt ctgaacgttg aaccgcgttt tcatcattgg tatcattccc
    60601 ctttgcgccg catataggca aacggcgtgt aaaagtcaag aaaaaaattc ccccgccggt
    60661 ttttttcgcg gctctcgcta tccgcgctca tcgattggaa tgtagtgccg cagattgctg
    60721 ccggtataaa tctgccgcgg tcggccgatt ttcagcggga cggtatcgtg catctccttc
    60781 cactgcgcga tcatgcccgg cagtcggccg atggcaaaca tgaccgtgaa catgttggtg
    60841 ggaatgccga tcatccggta caggatgccg ctgtaaaaat cgacattggg atacagcttg
    60901 cgctgaatga agaaatcatc cttcagggcc gcctcttcca gctccatggc gatattgatc
    60961 agcgggtcct ggtaattgtg cttctggaaa atggtttcgc agtattgttt cagaatccgc
    61021 gcgcggggat cgtagttttt gtaaacccgg tggccgaagc cgaaaagccg gaacttgctc
    61081 gttttgtcct tggccgaatc gatgcaggtt tggatggaca gcttgctgct ttggatttct
    61141 tccagcatct cgatcacttt ctggttggcg cccccgtgca gcggacccca cagcgcgcaa
    61201 atgccggagc agattgacgc gtacagattg gccatactgc tgcccaccag ccgcacggtg
    61261 gacgtgctgc agttctgctc atggtcggcg tgaaggatca tcagcgtgga gagcgccctt
    61321 tcaatgtccg ggtccggcac gtaggggttt accggagagt caaacatcat gtagaggaaa
    61381 ttggacgcgt atttcaggtc ccgtttggga taaacgtgcg gatggccgat gctgtgcttg
    61441 taggaatacg ccgctatcgt gcggattctg gagatcagcg aagccgccat cagatcgaac
    61501 gtctcggccc tgaaatcttc cgtgaaataa tccgtatagt aaacggacat ggagtttacc
    61561 atggtcgcca gaatcgccat cggatgggcg gtcggcggga acccgtcgaa gaaccgcagc
    61621 atttcctcgt gaatcatgga gttttgcgtc agccgctggg aaaaaagctg ctgctgctcc
    61681 cgattgggaa gctccccgta gatgagcaga taggccacct cggtaaagct tgctttttcg
    61741 gcgagttcct cgatgggtat tccgcggtat ctcaaaattc ccttgtctcc gtcgatgtac
    61801 gtgatggagg agatgcagga acctgtgttc tgatagccga cgtccagcgt aatcaaaccc
    61861 gtatcctgcc tcagcctgga gatatcgatt cccttttcgt tttccgttcc ttcaacgatc
    61921 ggcagctcgt atttcattcc attgagcgtc aacgttgctt tttccatggt atccttcacc
    61981 tttcgcaagc cttgaaatat ccggctgttt taagggggtt ctttacatga aaggcgttca
    62041 tctgacaaca cgaattttca tgacgcgaga cgatgcggcg ccttcgcggc tctttgcgcc
    62101 gcgatgaaaa ccgtcttgaa ttatcgattt ttaaatgcta gaagcactag cggtttcata
    62161 ataaaaagcc gtcaaaaacg gagaaaacaa aatgaaaaaa atgatgatgg ttcttttgtg
    62221 ccttgttgcc atcctgctga tggcggcgac agttttatcg aaggaaaaat acacggtaac
    62281 ggttttacct ttttcgctgt atagcgctga aaacattgaa tatgtgcggc aggggatcaa
    62341 cgacatgctg atcagccgca tctccgggtc cgacaggatt gaagtgacgc gcaaagatgt
    62401 tgtttcggac atcctgaaaa aaacaggggg aaaagagctg aacctggcgg atgttcaggg
    62461 catcggccag cagttgaaat ccgattacgt ggtctggggc agcattacca aaatcgggaa
    62521 aagcatcagc attgacggaa aactgattga tattgccgga gggaaatcgg acgtcggcat
    62581 tttttcccag tcgcagagcc ttgatgaggt cattcccaaa atcaacgatt tttcgcagcg
    62641 cattgtccgg catattaccg gaggggccgc cgcgcaaacc tcgccggttg tggctgccgc
    62701 cgcgcctgcc gcctcggctg ttgccggagg ctcccccgct cctccgccgg ttcccggcgc
    62761 atccagggaa gcgcagatca tcgccggcat gaagtccggc aagggcaaag gaacgctgac
    62821 ctcgatgatc aacgcggaat tcatcaactc ctccgatccg ctcgatcgca agaagggctt
    62881 ctggatgagc cagcaattca aaacggaatt ctacggaatg gacatcggcg acgtcaacaa
    62941 ggacggcgtc aacgaagtgg tcatcattga ccgccacaat gtctatattt acctgaagac
    63001 caagaataat ttacagttat tgaagcagat caaaggcaaa tcctaccata aatacatctc
    63061 cgtggacgtg gcggacatca acaaagacgg aacgccggag attatcgtga cgtcgctcaa
    63121 cgatcgggtg ctcaattcct ttgtgcttca gtacgaggac ggcgactaca agatgatcgc
    63181 gtccgatatc cggtatttcc tgagagtcat tacgccgtcc tcgggaaccc ccatgctgct
    63241 ggggcaaacc tacggtatgg gaagcttcag tgacggcatg ggcggcaagg tcttcgatac
    63301 gcaaatttat gaaatcgtct ggcgcgacgg gaaatacgtt gagggcgaca agcagaaaat
    63361 ccccgtcgga ttgtccattt acgggttgac gctcgattcc ctgggcgcgg ggaccagtga
    63421 aaaaatcatc gccctggatg agctcgacta cctgtgcatc atcacgccga ccaacaagcc
    63481 gttgagccgg attttttcgt tcggcttcag tcccgatgaa ctgatctgga gaagcgactc
    63541 ccaatacggc ggcagcaaca attatatcga caatgtggac aagcagaagc agcagtccac
    63601 ggaagccacg caggaggaag gcgctttcgc caatctgcgg atcctgatgt tcgacacgaa
    63661 caacgacggc aaaaaggaaa tgatcatcgt caaaaatctg tcctcggtcg gtcgcgtctt
    63721 taaaaacctc aagcttttct cctcgagcga aatttataat ctggagtggg acggcctcgg
    63781 catggcggaa aactggcgga caaagaaaat caacggctat gtcgcggatt actgctttaa
    63841 ggacgttgac aatgacggca aaccggagat tgttctggcg ctggtccagt ccgtcggtgc
    63901 gacgctcagt gagagaagcg ttctggtcgt gtatgaactg gaaatgccgc aataggtatt
    63961 gacaaacgcc ggactcttcg gtatagcctc cggcggcttc gcaaagaacg cttgcttttc
    64021 aaggcggtgt agctcagttg gtcagagcat acggctcata tccgtagtgt ccctggttca
    64081 aatccaggca ccgccaccaa ttcatccttc cgcgatgaca acacccggat attacaaacc
    64141 atttttcaaa acaggaaact catgagcgcc gaacccggaa accatcatgc aagctttcag
    64201 ccccggtcct ttcttaagaa ccggcacctg cagtccgttc tggcgtcttc gcacctgatc
    64261 attccccctc acggccccct gatcaggcat tccagggaat ttattatcga aacgcccggc
    64321 ggatcaaagt tgctgtcgta tctgtcgcct cacccccatt cccggggaat gatgattctt
    64381 ctgcacggct gggaaggctc ctcttcttcg gcttatatac tggccgcagg cgcctacttt
    64441 tttgaccgga atttttccgt ttgccgcctg aatttaagag accacggcga gtcccaccac
    64501 ctcaatagcg gcctttttca cggagcgctg attgaggaga cattcgacgc ggtaaacgtg
    64561 atcgcgcaac aagccggcaa tctgccggtt tatcttctgg gcttttctct gggcgccaat
    64621 ttttcgctgc gcatcgcgat gcgccattcg catacgccag tcacaaatct gcaaaaggta
    64681 ttcgccgtca gtccgccgct cgaccctttc aagacgacgc agacgatcga cggcggcccc
    64741 gctctgtacc ggagctactt tctgcgaaag tggaagcgtt cgcttcgaaa aaaacagaag
    64801 tttttcccgg atcattacga cttttccggc atgctgcggg cgcgctcctg catggaactg
    64861 acggaaaaaa tgatggccta ttttccggaa ttttcttcct atcgggccta ttttcagtta
    64921 tacacgctga cggaaaaatc tttccgtgac ctcggcctgc cggttaaaat attcattgcg
    64981 gacgatgatc cggtcattgc tctcgatgac ttccgcaagc tgcgcgacca cggttttctg
    65041 acggtttccc ggcagccgtt tggagggcat tgcggttttg tggacctgtt tccccagcgg
    65101 cgctggtaca atgaacaaat ttttactgag cttaaccaag agagaaacgg atgaccatgg
    65161 acttgacgct gctgcggcaa aaagacgcct ttccgctcca cgttctggag cgcctgaacc
    65221 ggacgcctgt tgactattta gaaaagcacc tttccattga acgctccaac gccgccggcg
    65281 ccggacattt ggaagccggc gttgtgctgc tgctgctgtt cagggattcc ggatttcact
    65341 ttttgctgat caagcgatcg gatgccgtcg cccagtccgg cgacatcagc tgccccggcg
    65401 gcatgctgga gcgccccgtc gatgaaatgc tgagccacat cgtttcgaaa accggcattc
    65461 tccggacggc cggcaaccgg gcgcttcacg aactcccgaa caaagacgag cagacccttt
    65521 cgctcatcca gctttttctg atgaacgccc ttcgcgaagc ctgggaagaa atcggcctga
    65581 gcccgctgaa tgtcgaattt ttgggcgccc tgcccaccta ctcgctcaca ttgtttaccc
    65641 ggaccatcta tccgctggtc tgcctggtca aagaccattg gccttaccgg cccaatgacg
    65701 aagtggacaa gatgctggaa atccccgtta cagctttttt ccggagctct tcctacgctc
    65761 ttttggagct caacacggag gatggaaagt ccgaccctcg ccataacctc cagttcccct
    65821 gcctggtcat tccggacggc aagggcggcg aagacatcct ctggggcgcc acgtttttca
    65881 tcatcaccaa tttcctgcgg gaggttacgg gcggcgcttt tccggccgaa acgcccgggc
    65941 gcatcgtcac caaaaccctc tctgcacact acacgtcggg aaaccgttga acccatggcc
    66001 cctattcctt tcgaccggtc ctccatcgac accgtcatct ttgattttga cggaacgctg
    66061 gcccggctga acatcgattt tgaacaaatg cgccgggagg ttgccgctct gatggaccgc
    66121 tggggtgtgg actcccggag tctgcaaaac cggtttgttt tggaaatgat cgccgaagtt
    66181 cagaccctgc ttggaaaaaa ttcaggaaac catgcagcgt tattcacgcg ggaagccttc
    66241 cggatcatcg aagagattga agtggcggcg gcgcaaagcg gatccctgtt tgacggcgcc
    66301 aaaggacttc tcacgggtct caggaaagcc tcccttcaga cgggaattat cacccgcaac
    66361 tgcgaaaaag ccgtccggac cgtctttccg gatatccggg actattgtcc ggtggtcatc
    66421 tgccgaaacg acgtccgtcg tgtcaagccg catcccgagc agatcaatct cgctctgtcg
    66481 agactaagcg caaaacccgg ccgctgtgtc atgatcggcg accacccgat cgacattgaa
    66541 acggggaaga acgcggggac ccggaccgcc ggtgttctga ccggccgctt tcaggcagag
    66601 gattttctgc aggcgggagc ggacctggtg cttgcggaag ccccggagat cctgcgacgg
    66661 ctgaagccgg agcgctgttg acgcttcttc tctgattgac atccccggcc aaattgtata
    66721 gaagcggacg gcgccggaag cccggacgca tcatcttgct tgattttgag aggaaaaatg
    66781 aatccacatg tttttcgcga atacgacgtg cgcggcgtcg ttgacaagga cctcaacgag
    66841 gtttttatcg tcaatctggg tcgcgccatc ggcacgtacg cgctacagcg caatgtccgg
    66901 accctgacgg tcggacgcga ctgccgcctg agttcggacg actaccgccg ttttctgatc
    66961 gcggggttga acgcagccgg aatagacacc atcgacatcg gcctgtgcgc gacccccatg
    67021 ctgtattttt ccatccgcca ctgcaatgcg ggaggcggcg tcatgatcac cggaagccac
    67081 aacccgccgg agttcaacgg ctttaaaatc tgcgtcggat acgattccat tcacggggaa
    67141 caaattcagg aattaagaag catcatggaa tccggaagtt atctttccgg caagggaacc
    67201 gcgcaggcgc aggatattac cagggtttac caggactata tcttcgacgc cgtccgcgtg
    67261 tcgaaaaaac tgaagattgt ccttgacggc ggaaacggcg tcgggggcaa atttgccttt
    67321 cccattctgg aaagaatggg ctgcgacgtc atcgccctct attgcgagcc ggacggccgt
    67381 ttccctcatc attttccgga tcctaccgtt gaggaaaatc tgacggatct gatccggctg
    67441 gtggccgaaa acaaagccga cctgggcatt gccttcgatg gcgacgcgga tcgcatcggt
    67501 gtgatcagcg acaccggcga gatcatctgg ggagacaaac tgctgctgtt gtttgcgcgc
    67561 tttatcctga aggaacatcc cggggccacg atcatcggcg aagtcaagtg ctctcaggtc
    67621 ctctatgacg gcattgggca atcttccggc aggccgctga tgtggaaggc gggtcattcg
    67681 ctgatcaaag ccaagatgaa ggaggaaaaa gccctgctgg ggggagaaat gagcggtcac
    67741 ctgttttttg ccgaccgtta cttcggctat gacgacgcga tttacgccgc cgccagactg
    67801 ctggagattc tctcccagac cggtctgaag ctctccgaac tcctcgccga cgtgccgaaa
    67861 accttcgcca cgccggaaat ccgaatggat tgcgacgatg accggaagaa agcggtggtg
    67921 gccagaatca aagagcatta ccgcaacacg cccggcgtca ttgacattga cggcatccgc
    67981 gtgcccttcg gggacggctg ggcgcttgtc cgctgctcca acacacagcc ggtcatcgtc
    68041 cttcgctttg aagcgtcatc cgccgaacgc ctgcaggaga tccgcggcga agtggagcgg
    68101 ctcctggatg gggtcaggtc ttgcaatcac gcacggaagt cctctttgac ggagcaatca
    68161 tgctgattgc ttctgaatat tgcggtgcat accgcaagta tgaaccggcg atagttcacc
    68221 atccctcaat ccctcccatc aagggaagga agtaacaaac ggccattatt aggatttctt
    68281 gtcgctcgcg ctccccgaaa tgacattttt caaaagtctc catctgcaat ccggggattg
    68341 catccggtcc ggcctcactt ttgtttcgtc ctcaacagca tgaaggttgc ggaagcgcgg
    68401 cagaccggtt ttcccccgtc gtccgtgacc aggcaatctc cgatgatgag gcttcggcct
    68461 ttcttgaaaa cactcgcttc ggcccgaagc tttcctttgt ccacaccggc cagaaaattg
    68521 cttttcagct cgatcgtcgt cagtccttct tccggggtga gcaccgtcat catggcgtgg
    68581 gcgatggcct cgtccgccag tgcgaccatc aagcctccct gcatgaatcc ggcgccctgc
    68641 aggtattccg gtcttacggg catccggaag acggcgcggc cgggcgacag atcgaccacg
    68701 tcgatgtcaa aatgatccag atagggattc aacccttttt tcccggcgcg aattttttcc
    68761 agataaagat gataattcat gatggcgcac cccctttttt tttgacggaa atgacctttg
    68821 aatcctcgca acttataatc ccagccccga aaaaatgcca acagtttctc cgcatcagac
    68881 ccggaccgga atgggatccc caaaagaccg ttttgcctga ccggagcaaa aatacagcgc
    68941 cgcgccaata atcatattga ctaaagtttc ctttttgaat agattcactt cggtttattg
    69001 tcttgaaacc acccggaaag ggatttttat ctcgttcaga acgaaaacaa atcggataat
    69061 caacggttat ccacaatatt ttatcggaag gagatggagt ttttatggag cacactcatg
    69121 aagtggaaca aatgtgcgta gttgccaagg gcgccaaaaa cggccccgct cccataccgc
    69181 aggagggcaa atggacgcag gtaaaagaag tcaaggatat cagcggcctg acccacggca
    69241 ttggctggtg cgcgccccag cagggcgcct gcaaactgac cctgaacgtg aaaaacggaa
    69301 tcatcgaaga ggcgctgatc gaatgcatcg gctgcaccgg tatgacccat tccgccgcga
    69361 tggccgccga aattctgccg cataaaacgg ttctggaagc gctgaattcc gacctcgtct
    69421 gcgacgcgat caacaccgcg atgcgggaaa tattcctgca aatcgcttac ggccgcacgc
    69481 agacggcctt ctccgaaggc gggcttccca tcggcgcagg actcgaggat ctgggcaaag
    69541 gccagcgcag cgtgattgcc acgatgtacg gcaccaaagc caaaggcccc cgttacctgg
    69601 aaatggccga agggtacgtg ctggaaatcg gcctggatgg cgacagtgaa atcatcggct
    69661 acaaattcgt taatctgggc gtgatgatgg acagcatcgt caagggcatg gacccggccc
    69721 aggcgctttc gaaagcaacc ggaacttacg gccgttttgc tgaagctgta aaagttatca
    69781 atccccgtaa agaataagga gtagaaaaat ggctttattt gaaggatacg aaagaagaat
    69841 caaacagatc actccttttc tcgcgaaata tggaatttcc tcgctggagg aagccgaaaa
    69901 agtatgcctc gagaaaggcg tggatgtcag aaaaatcgtc aaggaaattc agccgatttc
    69961 atttgaaaac gccggctggg cttatctggt cggcgctgcc gccgccatta aaaaaggcca
    70021 gaagacggcc gccgatatcg ccgaaacgct gggtgaagga ttgcagtcct tctgcattcc
    70081 cgggtccgtc gccgacgacc gccaggtggg catcggacac ggcaatctcg cttccatgct
    70141 gcttcgggaa gagacaaaat gctttgcatt ttgcgcgggg catgaatcat ttgccgcggc
    70201 ggagggcgcc atcggactcg ccaaatcggc caacaaggcg cgcaaagttc ctctgcgcgt
    70261 cattctgaac ggtctgggaa aagacgcggc tcatattatc gcccgcgtca acggctttac
    70321 ccatgtcgcg accgcatttg actacaaaac cggaaaagtt tccgtggcca gggagaaagc
    70381 ctactccaag ggcgaccgcg cggccgtgag gtgctacggc gccgatgacg tgcgcgaagg
    70441 cgtggccatc atgcatctgg aaggcgtgga tgtggccatc acgggcaact ccaccaaccc
    70501 gacgcggttc cagcatccgg tgatgggcgt ttacaaaaaa gaatgcattc tggccggcag
    70561 gaagtttttc tccgtggctt ccggcggcgg aacggggaga acgctgcatc ccgataatat
    70621 ggccgccggc cccgcctcct acggcatgac cgacaccatg ggacgcatgc attcagacgc
    70681 gcagtttgcc ggatcatcct ccgttcccgc gcacgtggag atgatggggc tcatcggcat
    70741 gggcaacaac ccgatggtcg gcgcttcggt tgccgtcgcc gtcgcggttg aacaggccat
    70801 gaaataatcg gggattttca aaaatggaca cacagcggtc ttatgccgcc cgcaagggcg
    70861 gctgcggccg ctcttttttt acccggaaga aatattattt ttcccggcgc atgacgtgaa
    70921 cggccgtggc cttgaaggac gcaaaaaccg ttgcgccctc gcacagcccc agatcctcgc
    70981 aggaagacac ggtgagatag gcaaccacgg gaaaaccgca gtccagaacc agtttataaa
    71041 aaaaggcctg gcggacaatc ttctgaattt gagcctcgaa gatgtttcgg gcgctcgaat
    71101 tttccggcgc ttcccgcgac acaacgatat tttccggccg gatgcagcaa aagactttct
    71161 ggctgatcgg agcgctgccc gtcgcctcga tggttttccc cgacacaagc acttccatca
    71221 gacccgaccg gatgttttgg acggttcctt ccagaatcgt ttcggttccg acaaaattgg
    71281 ccacaaactc ggattggggc tgattgaaga tctgcgcggt cgcgccggtc tggatgagtc
    71341 gtccttcaga catcaccgtg acatcctgcg ccaggcgcag ggtttcttcc cggtcgtgca
    71401 gcgccagcac ggcggtgatc ttcgtctctt ccagaatatg ccgcagatca tcgatcagcg
    71461 attcgcgcgt cggcggatcg agggagttga acgcctcatc gagcaaaatc agatgaggct
    71521 tcagggcaaa ggcgcgcgcc agggatactc ttttggattc tccgcccgaa agcgttttgg
    71581 ccgatctttt cgccagcgaa ctgatgccga aatacgccag ggcgtcttcg acgttcttcc
    71641 gaatgtcgct ctgcgtcatc ccgcggaact tgagccccag ggccacattt ttataaacgg
    71701 acgtggaaag cagcagcggc tcctggaaaa caacacaaac gctcctgcgc atccaggaac
    71761 gctccgcgct ggtttcaatg gatcgtccct tgtaatatat tttcccctgc tccggtttga
    71821 gcaggcctgc cattgtcagc aacagcgtgg acttgcccgc gccgttgggt ccgattaaag
    71881 ccagaactct gctttcctga accttcagat cccgaatgtc cagcaccctg acgccgccgc
    71941 ggtgaaccat taaattttcg acgctcagaa tcgtgttcat cgtggatgct gcctttgctg
    72001 aacgtgggtc agcagcagat tcaccagaaa cgcaaggagc aaaaggataa tgcccagcgc
    72061 tatggcgata tcgaagttcc ccttgccggt ttccatcacc gtcgcggtcg tcagcacgcg
    72121 cgaatatcct ttgatgttgc cgccgaccat gatcgatgct ccgacttcag aaatcacgcc
    72181 cccgaaaccc gccatgacgg ccgccaaaag cggaaggcgg gattccttga tgagaaccca
    72241 gaccatctgc atccggctgg cgcccagcga cagaatctgc agccgcagat tgggcggcag
    72301 attctgaatg gaggccagcg agatccccat gacgatcggc gtggcaatga tcgcctgggc
    72361 aatgatcatg gcgtagggag tgtagagaat ttcaagaaaa ccgaatgggc cgctgcgcca
    72421 gatcatgatg gtcacaaaaa gacccaccac cacgggcggc aatcccatgc ccgtgttgat
    72481 aatgctgatg accagccttt ttcccggaaa gttgtttaac gcaacgaccg tcccgacgga
    72541 aacgccgaag aacagactga tgaacgtggc tgtccccgat attttcaggg acagccacgt
    72601 gactcccagc acttcgggat ccaggctgat cagcagttga aaagccttga cgattccctg
    72661 aacaattaat tccatcgttg attattttcc cagatcttca actttctttc cggcatccgg
    72721 gaaaaataaa ggcgaaccgt atttatccac gccaaatgtc ttaatgatgg tctgcgtttt
    72781 tttggaaacc ataaaatcgg caaaggcttt ggcgcctggg gcattggctt tcggccactt
    72841 tgcgccgttg acttcaatca catggtaaat gttgagcagc gccgcgtcgc cttcattcag
    72901 aatctccagc cccagatttt ttctcatggc cagataggtg ccccggtccg acagtgtgta
    72961 ggcttttttc tcggaggcga cgttcagcgt ctgtcccata cccagccccg tttcctgaaa
    73021 ccatttctgt cccgacggat tgatgttcgc ctttttccaa agggtctttt cctttgcgtg
    73081 tgttccggag ttgtcaccgc gcgaaataaa cagagcgttg gcggcggcaa tcttcttcag
    73141 cgcgtccgag gaggatttga tgcccttgat tgcggccggg tcggacggcg ggcccacaat
    73201 gatgaagtca ttgtgcatca ccaggcggcg gttgattccg tagcccgctt caacaaactt
    73261 cttctcggca tccggcgaat gaaccagcat aacatcggcc tcgccttttt cccccatctt
    73321 cattgcctgt ccggaaccga cggcgatcgt cttcacgaag tagcccgtct ctttttcaaa
    73381 aaggggaatt aaaacatcga gcaggccgga atcctgcgtg ctggttgttg tggccagaat
    73441 gatgtttttc tgcttggggg ccgcgaacgc accggttgcg cccagcacaa ccagagacgc
    73501 gacgacggcc aggaggataa accgcttgct gcttttgaat attttcatat cttgtctcct
    73561 tcttcaggtt ttatttgttc tgcagtttct gccattccac ggaattgggg aaaaacagcg
    73621 gcgcgccgta tttgtctttg ccgaaatccc ggatgatcaa ctggcctttt tccggtgacg
    73681 tcagccactt gacgaacgtc agggtgtctt tgtggttgac ttttgggaat ttggccgggt
    73741 tgaccggtat cagcgaaatg taattcaaca gcgcctcgtc gccttccacc aggatgacca
    73801 ctttgatctg atctttcagg gacaggaagg tggcccggtc aatcaccgtg tatgcggcct
    73861 gctgatcggt gtatttcagc gtgggaatat ttccttccgc ccccttttca taaacaacat
    73921 accatgcgcc cttaggttcg acacctgatt ttttccagag ttccatttcc gccacatgcg
    73981 tgcccgactt gtccccccgg ctgatgaatt tggcgtttgc ctccatgatt ttcttcaagg
    74041 ccgccgttgc cgatttttct cccttgacgg cagccgggtc tgaagcgggg ccgacgatca
    74101 caaaatcgtt gtacatgagc ggaattcttt ccgtgccgta cccgtcggca ataaactttt
    74161 cctccaggga tttggcatgc gccatcacca gatcgatctg gccgttggtg gccagcttga
    74221 gcgccgcacc ggtcccggcg ccgacgtggc gaacgcggat tccggtttct ttttcgaact
    74281 gatcttccag cgcagccaca atgccggagt cgatcggtcc gatcgtactg gataaaaaga
    74341 tgaacggctc gcttttcggg gatgacagag acgcgcaccc cgccaccaga accaggcaaa
    74401 gcaaaacaac caacgccgac cattttctga caaaaatcct cataatcatg ctcctttcac
    74461 ttttcatttt tcgcacgttt tcgtaatgca ttatgagaat tttatcaaga atattatccc
    74521 aaaatctgac gttaaattac ctttttgcca attatcggcc caataccatt tagaatttgt
    74581 tgatgggaac attttacccg ggtggataca gaagatgggg agaaaggaag cagggaaaga
    74641 ctgaaagtgg ctagtg
//
LOCUS       DAOSRM010000002        65305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_468_length_65305_cov_107.334008, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000002 DAOSRM010000000
VERSION     DAOSRM010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 65305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65305
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_468_length_65305_cov_107.334008"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1095
                     /locus_tag="PLA58_00350"
     CDS             7..1095
                     /locus_tag="PLA58_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOR61272.1"
                     /translation="MRKNFASRFPDGTSRNWRDWRWQLRHRATSLIALTRILETPPRD
                     GKPLERVMSRYPMAITPYYLSLLEPDHENDPLSLQCVPDLRELSFSAGLRDDPLGEDR
                     SMPAPNLIQRYPDRVLAIVTHRCATYCRHCNRKRFWSVPEAGPLKNRLAPMVRFVSES
                     PQIREVILSGGDPLMFDDASLEWILSSFASIPHVEVLRIGSRAPVVLPMRITGNLCRI
                     LKRHRPLWFNTQFNHPSEITKDAQRACNMIQEAGIPVSNQSVLLQGVNDSPKVMQSLL
                     NGLQKISVRPYYLFHCEPARGCNHFRTDARKGLNLMEKLHRSCSGLALPQYVADLPGR
                     EGKVPLWPLSRPIQNALRKHQDFFDNFE"
     gene            1158..1721
                     /gene="efp"
                     /locus_tag="PLA58_00355"
     CDS             1158..1721
                     /gene="efp"
                     /locus_tag="PLA58_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOR61273.1"
                     /translation="MYTASDLRKGLRLKIDNDPYVITEFNFVKPGKGQALYRCKLKNM
                     LTGSQFERTFRSVDVFEPADLREKKMQYLYKEGDKFCFMDNETFEQVYLTQEQVGDAV
                     NFLLDNIEAEILLFEDRPIGISLPNFVELVVTEAEPWAKGDTVSGATKPVKVQTGYTI
                     LVPTFISEGEKIRIDTRTGEYLTRVKG"
     gene            1857..2795
                     /locus_tag="PLA58_00360"
     CDS             1857..2795
                     /locus_tag="PLA58_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase GenX"
                     /protein_id="HOR61274.1"
                     /translation="MSTLPKDDCFYLARKAQALQLRAGFTEHLRRFFIQQGFLEVETP
                     LRIPAPAPEEHIEPLPSGNWFLQTSPEICMKRLLAAGYPRIFQFCKCFRAGERGNRHL
                     PEFSMLEWYALHCDYRKLMDQCEDLLISACRQMGRSGKIVWQNKTIRLSPPWERITVA
                     DAFSRYAPVSLPNALAGDRFDEVLVEHVEPNLGNDLPTFLFDYPAQMASLAKIKKDDP
                     AVAERFELYIGGMEIANGFSELTDAREQRRRFEEALKAQAARHQVHYAMPEPFLASLE
                     NLPPCAGIALGLDRMIMILADTATIDDVIAFSPETL"
     gene            2903..3598
                     /gene="tolQ"
                     /locus_tag="PLA58_00365"
     CDS             2903..3598
                     /gene="tolQ"
                     /locus_tag="PLA58_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOR61275.1"
                     /translation="MESNFHGSLLGMILDAGLMVKFVLLILLVFSIISWTIIFIKFRT
                     YRRIRQENEAFDSDYQKSTKLSDLLPTSRKYPFSTTAEVFRAGYAEMTKANRLSRDSA
                     RPEEISLSSLDNVERALNRASSTEMTKLESALGFLATTGSASPFIGLFGTVWGIMETF
                     KSIGARGSATLAVVAPGISEALIATAAGLAAAIPAVIFYNYFLNKSRTMVQEMDNFAD
                     EFLNIVERYLIRK"
     gene            3658..4041
                     /gene="tolR"
                     /locus_tag="PLA58_00370"
     CDS             3658..4041
                     /gene="tolR"
                     /locus_tag="PLA58_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HOR61276.1"
                     /translation="MAEINVTPLVDVMLVLLIIFMVTAPMLSMGIDVNLPRVKSKSID
                     LAEEKLILSINENREIFLNKTRLPVQDLNAKLAAIFAGRVDREVFLRADKNVPYGFVV
                     EVMSEIRKAGVDKLGMITEPPGDSK"
     gene            4050..4760
                     /locus_tag="PLA58_00375"
     CDS             4050..4760
                     /locus_tag="PLA58_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOR61277.1"
                     /translation="MTGKRVFGKNSGAGNMFLASLFLHLMVIAAVFATVPGSSKQLTF
                     GAPYSVALVGPDVLESSKEDVPKPKDLLQSGASTGPVILKRDIGASPSSFVMKKDDSG
                     KPDIEKAIRALEQKETAAGQSKDSPAASTSPIAAAANSSQLNEYSRHVWSKVKRNWSL
                     PAALMPKNNVEAVIEVRIAKSGAVEHIGFEKRSGNSYFDESALRAVKKSTPFPPLGGW
                     TSGRTVEIGIRFHSAELR"
     gene            4787..6103
                     /gene="tolB"
                     /locus_tag="PLA58_00380"
     CDS             4787..6103
                     /gene="tolB"
                     /locus_tag="PLA58_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HOR61278.1"
                     /translation="MDRKYRLAVGTLLVLAWIFNAAGAAADKVYIDIDAPGFQQFPVA
                     ICDFQIKTGDPSKESVDLSDSVRRYLDLTGIFRVLDKKSFLEGVEPGSPEIIRFSDWS
                     VIGADFLLRGNLTVRGKDVFAESRLYDVTRGQMLFEKKYSGITDDLQSVALSIASDIL
                     LALTGDPGDFHTKIAFVIKKKSSSDIFTMNYDASDVKNLTNDHSIVAAPRWSPDGRYF
                     AYTSYKNGRPMVFLRHLKTGKEKILASFSGLNLCGSFSPDSKKILLTLSKDGNEEIYV
                     LDIETMNLRQLTHSFSIDVSPAWSPDGRKIAFVSNRSGSPQIFVMEADGNNVKRITFE
                     GNYNTSPSWSPRGGRIAYEGLINRKYQIFSVDDEGNNLIQLTFDDADNEYPSWSPSGR
                     QIVFHSRQNARSRICIMNANGMNFRILKESGDFLVMPAWSPRSEQK"
     gene            6196..6828
                     /gene="pal"
                     /locus_tag="PLA58_00385"
     CDS             6196..6828
                     /gene="pal"
                     /locus_tag="PLA58_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOR61279.1"
                     /translation="MKKHLTVIGLFLILAFSLTIFSGCAEKKAVVKDEAIQEQKVAAA
                     QEAAPAAVTADDEAARKAKEAADREAALKEQAAKDEAARREAARAAWAKKNAEALVEL
                     SLQNIYFDYDKSAIRPDAREILKANAEIFTKNSSAKIVVEGHCDERGTAEYNMALGER
                     RAQEAKQYLVNLGIDASRIETISYGKERPLDNRSTEEAWAQNRRAQFLLK"
     gene            6854..7678
                     /gene="ybgF"
                     /locus_tag="PLA58_00390"
     CDS             6854..7678
                     /gene="ybgF"
                     /locus_tag="PLA58_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOR61280.1"
                     /translation="MKRFWHLILPAMAFSLAACATSSDLKELRTELNQQMEAKFAAAD
                     TRLDDRVSALQKAQAKNAEDLSSLRKTNANTNANFSDLRDQVQQLRGQVETLKKETSR
                     DPKKDAEMQEKIDHLLVKINFIENFLEIGKKDAAGAADSAASGKDQGKQGKAAVYAAA
                     YQTFKNGHYTKAREAFQDFLTAYPTGEYSDNAQFWIGECYFFEKNYEKAILEYEKVTK
                     KFPSSNKVPYALLKQGLCFQNLGDKVSAKLLLQQVIRDYPNTSQARIARSKLQEIK"
     gene            complement(7731..8510)
                     /locus_tag="PLA58_00395"
     CDS             complement(7731..8510)
                     /locus_tag="PLA58_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOR61281.1"
                     /translation="MFYDFASGKRMTGILLLSLLACGCAGGAVTESGRHPDPAAMLRP
                     ALRETHRIVATARMDLSTVQGHYPLRAALILQRPSYLRLEMLPVIGTPDFFLSATPRE
                     MRIWIPSQGEFYVGQPSAENMSRFLPWTLNLEDLVMILSGSFPPLDGAHWSYGDSAEG
                     GALRVDMKTVSGPSQTIWMENSGRLTRFVRYGSDGREVYSVRYEDYTPGLFLARKILI
                     QRADNTLSAFVEYSDVNIEETADFSVFALPAPDGAKILRLD"
     gene            complement(8553..10253)
                     /locus_tag="PLA58_00400"
     CDS             complement(8553..10253)
                     /locus_tag="PLA58_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR61282.1"
                     /translation="MQKKHVFFLICGFLFAAILAGCASFPASRPPADAPVPDPYSKEA
                     ACHYSLAVLSRLDGNLDEAIDQMKRALSFSPDSSYLTTELVSLYVENNDVDPALSLGE
                     SALAKDPGNIELRSILGGLYFNLREYDKAVRAYQTVTEMDPENAVAYLYLATIYSQEK
                     KYDQAEKAYQKLLALDPDHIIGMFYYAKTLTQMNRTEDAEKLYRKIIAQRPSFEAAWT
                     GLAALYENQNKHEEAIVVYRRYLEMNPARIGIRVKIAELLMKTDREEAAEKELREVLK
                     ISPENREVQMALGLLYYDIRRYDQAAAQFSLLLETTPADDKLRYLLASVLEQKGDLQK
                     ALTEYQKISPKFELYANAQIQAAKLIKQEGDFTGAIAVVRRAIVQRKDQAILYLYLSS
                     LYEEQKDMAAAEKTVREGTGLFPGNTDLHYLLGALLEKTGRFEESIQSMKQVLALDPQ
                     NADALNFIGYSYADRGMRLDEAEQMIVQALKIKPDNGYILDSLGWVYFKKNDYESALK
                     YLKKALGFLPEDPSVMEHLGDTYLKIGRREEALEWYRKAIKVDPGNSRLKDKIDLLKP
                     "
     gene            complement(10298..11206)
                     /locus_tag="PLA58_00405"
     CDS             complement(10298..11206)
                     /locus_tag="PLA58_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOR61283.1"
                     /translation="MKKKKIQKTTTVLITLIVLAVAAIAAIVLWDREETTGVKPPHLP
                     KEQAEGLREAAKAQRHKTEDGAQLARIAPPRSAARQVAIVIDDIGYDMKPVRELLAIN
                     ENITFAVLPLLAHSREAAETLHRAHREILLHLPMEPLSYPKEKPGRGALFTDMNDEEI
                     LFQLQRNVDSVPYASGVNNHMGSKFMADEEKLTLVFRELKKKNLFFIDSRTTRDSKTE
                     AAAHKARLTVMSRKIFLDNERDTSKIYQILMEAARSSDGNSPLIVIGHPYPETIQAVR
                     EAGKVCREQGITIVPVSALLGKQGAS"
     gene            complement(11241..12530)
                     /locus_tag="PLA58_00410"
     CDS             complement(11241..12530)
                     /locus_tag="PLA58_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOR61284.1"
                     /translation="MAVILFGLFVVFGPYSDSKVSARDKNIYKEIKTFNEVLDMVQKN
                     YVEEIDSSVLIQGAINGMIKSLDPHSSFMTPELYKELEVETQGRFGGIGIEITILKDV
                     LTVVSPIEDTPAFKAGVKPGDSIVKIDGKSTKDITIMEAVKKLRGPKDTKVTITILRE
                     NMAQPRDITLTRAVIQVKSVRAKAFDDHIGYIRIASFHERTADDLRKALTDVAEKTKP
                     MKGLVLDLRNDPGGLLTQAIEVSDMFLKSGVIVSTRGRTKKMETKATARDSGDKEVNA
                     PIVVLVNEGTASAAEIVAGALQDNGRALIVGTQTFGKASVQTVIPLEDGSALKLTTAR
                     YYTPNGRSIQAEGIKPDIIVKPVRPSEEPESLMGERLREKDLKGHIKPAKEEGAHPDE
                     NKNGDSRETTDLVKDNQLKAAIDILKSWDIMKKNLAK"
     gene            complement(12732..14204)
                     /gene="cysS"
                     /locus_tag="PLA58_00415"
     CDS             complement(12732..14204)
                     /gene="cysS"
                     /locus_tag="PLA58_00415"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOR61285.1"
                     /translation="MAQKIYNTKTRKKEIFQPVREGAVGMYVCGITAYDVCHIGHARA
                     AVVFDVVFRHLKARGYDVTYVKNFTDIDDKIIDRANREGVGIAEIAERYIALHDEDMA
                     SLNVLTPTVTPRATGHMEDMIDLIRALEEKGIAYAVDGDVFFAVAKFPAYGGLSGRNP
                     DEMMAGARVDVNDKKKNPLDFVLWKKSKEGEPSWDSPWGMGRPGWHIECSAMSRRYLG
                     EPFDIHGGGEDLIFPHHENEIAQSQAATGKPPVRYWMHNGFVKINAEKMSKSLGNIFP
                     IREIVRQFHPEVLRLFMLQSHYRSPVDYSDVSLREARTALIRCYTALRLMKDAQAADV
                     HLGGVKTDSDGGKYAARLRASVEKFDLALDDDFNTAQALGFVFDAARLVNQVMTVEKK
                     MPPADKQAILETAREAFRHFGDALGVFQSDPDLFFSKDRDIEAEKRGMDIHRVEALIA
                     ERQKARDAKDWGRADEIRRELALQSIALKDEAGRTTWIID"
     gene            complement(14218..15258)
                     /gene="argC"
                     /locus_tag="PLA58_00420"
     CDS             complement(14218..15258)
                     /gene="argC"
                     /locus_tag="PLA58_00420"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOR61286.1"
                     /translation="MKTKAAIFGASGYTGQELIRILSAHPQVELTAVTSRRYAGMKVS
                     DVFPSLSGLTFLTYANAAPREIAELCDVVFLALPHGVSMEIAPVFIEAGKKVIDLSAD
                     YRLRDRATYEQWYAKHSSAGLIKQAVYGLPELYREAAKTSPLIANPGCYPTGIILGLA
                     PALQSKLLDVSTIIADSASGVSGAGRDPVVGSLFCEVDGGFKAYKVGKHRHTPEIEQE
                     LHQLAGEKFFISFTPHLLPVKRGILSTMYATLKKKTTEAKLRDLYASFYAGEKFVRIC
                     PAGVYPNISSVCGSNFCDIGLTVDLRTGRVIIISAIDNLIKGAAGQAVQNMNLVCGLP
                     EDSGLGTPPLYP"
     gene            complement(15303..15695)
                     /gene="rpsI"
                     /locus_tag="PLA58_00425"
     CDS             complement(15303..15695)
                     /gene="rpsI"
                     /locus_tag="PLA58_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOR61287.1"
                     /translation="MTVQRFYATGKRKSAIARVYMKSGSGSIMVNKRNYEEYFTRPSL
                     KMIIRQPLEITGKKDQFDFYVNVSGGGVAGQAGAVKHGISKALLECDAELRPILKKAG
                     FLTRDARIKERKKYGQPGARKRFQFSKR"
     gene            complement(15739..16170)
                     /gene="rplM"
                     /locus_tag="PLA58_00430"
     CDS             complement(15739..16170)
                     /gene="rplM"
                     /locus_tag="PLA58_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOR61288.1"
                     /translation="MTKTFSAKAGQVKQDWFVVDASGKILGRLASEIALRLRGKHKAI
                     YTPHVDTGDFIVVVNAEKIALTGKKLTDKIYYSYSGYPGGLRETAAGKMLAEKPENLI
                     RMAVAGMLPKNHLGRKMLKKLKVYSGNAHPHEAQCPQTLTV"
     gene            complement(16344..18581)
                     /gene="lptD"
                     /locus_tag="PLA58_00435"
     CDS             complement(16344..18581)
                     /gene="lptD"
                     /locus_tag="PLA58_00435"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOR61289.1"
                     /translation="MMKILPVLLKRHTVMILGVIFVLGFARSGVWAQDVKAPDDAPVK
                     IEADTLSYDQERDLYEAEGNVVITYGDGVLTAASASYNRKNNLATARGNALLRMAQDS
                     LQGDKIVVNVEDKTGTAYNSKAFYARNHFYIRGSRIEKTGENTYFIEEPVATTCDGDR
                     PDWEIAGRSMNVTLEGYGLMKDARLRARHLPIFYTPLVAFPAKTKRQSGFLFPYLSHS
                     KDKDGIDIELPFFWAITPQMDATFFSRYMEKRGFKEGVEFRYFAGSKSFGTLYGDFME
                     DNRHVTETAAVGSQSRDWQETHRRWSYYLNHQTNFDSQFYVRTDLRRVSDSWYFRDFS
                     AHNYYLSNYSTTELDPFRKVPFQGNESLPSLESSARVYKGWNNFSLMARVSFTDDFSA
                     VNNERTLQQYPEIVLTGIKQRFFSTPLYYEFAGNYDYFYRGEGQKGHYVDIAPTISLP
                     FNISRYAKITPQITVREVYWNRDDAQANSDDKSGNRNVYNAGLSLSSRISRVFAVKIQ
                     NWDKIKHEIKPEIFYSYIPSVNQDNLPDYIPWVNSFMANSLSSNIGYSSVLKEQNAVA
                     WALTNTVTARTQDKSGSRSYIELLRLKLFQVYDINEAKKDMAGSTAERRPVSDLGIEF
                     DLKPHPYVSFAARNKYSPYVGWKEMNYDLGLNDWRGDNLTVGYRYTLDSLEEINMALK
                     LNLTRGFSGNLILRRDQFNDRTVENTVGLAYWAQCWGVGVDYTKTYDDERIMLKFSLA
                     GLGRF"
     gene            complement(18640..19935)
                     /locus_tag="PLA58_00440"
     CDS             complement(18640..19935)
                     /locus_tag="PLA58_00440"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOR61290.1"
                     /translation="MNSLNPGAYLSGLNIDVMRFGLEAITGLLERFGAPQNDYFTLLI
                     GGTNGKGSTAAMTASMLREAGFRTGLYTSPHLVDVRERIVLDGKKIPGRDLGRILSEI
                     SVKAPEPVSYFEALTAAAFIYFQRARADIAVMEVGLGGRLDATNVCRPLASVITNIGL
                     EHTAYLGRTLAAVAGEKAGIIKQGGVCITGATQKRVADHLASVCRRKKARFYRLGKDF
                     EAIRRRDGTVDYSGINRKISSLRIALSGGHQLANAALALAALEVAEESGLKIRDAAIR
                     AGLRKTRWEARMEILHTRPVFLLDGAHNPSGVRVLRRALEEDFSFRRLILIFAVLSDK
                     DYRNMLQKIAPLASVAILPPLPTGRAVPPEQLAGLMKKMGFQAVVAGTVDEAVSLALA
                     MAEEGDMICALGSLYLAGEVKQSFHQMNSCGKARRTEKK"
     gene            complement(19951..20718)
                     /locus_tag="PLA58_00445"
     CDS             complement(19951..20718)
                     /locus_tag="PLA58_00445"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HOR61291.1"
                     /translation="MKIRVLGCHGSQLPNHNTTSFLLGKNILIDAGAITQVLTLRQQL
                     LIDYILVTHAHLDHVRDIMFLADNLCYAKRKTPLRICSSRGILESIHRHLFNNVIWPD
                     FSRIPSAKNPLVKFEILSPGRQKTLGDWRVRAIPVHHTVETMGFLMESQGKEALFLGD
                     TGPTEDVWKVAGAAKGLRAIFVETSLPAGMQSLADKTGHLTPLTLAAELKKLKGAIPD
                     IYLYHMKPRYAGIIHREVASIPERKIHVIKDGQVIHL"
     gene            complement(20977..22692)
                     /locus_tag="PLA58_00450"
     CDS             complement(20977..22692)
                     /locus_tag="PLA58_00450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR61292.1"
                     /translation="MNYLLQPKKYQSLMSDEGSKAIMDKTIAFFEKMGKTRITEDYNK
                     KVWYREFVDFIGKEQIFAKLLTPSQYGGGDPDCRWDTARNSEYSELLAFYGLGYWYCF
                     QVSILGLGPIWMSPNEKAKQKAADLLKKGAIFAFGLSEKTHGADIYSTETSLTPKPDG
                     TWVANGEKYYIGNGNEAEMVSTLGKVKDGTDDYTFFVTNYRHKAYELKKNVISHQEYV
                     SNFALHDYPITEDDILSRGPHAWDSALNTVNIGKFNIGPASLGVAEHCFYESITHAAN
                     RVLYGIPVTAMPHVKKNFMDAWLRIVGMKLYQRRATDYFRNANAQDRRYLLYNPTSKM
                     KVTLQAEDVINLLWEVIAAKGFEKDTYFSIGASDIRGPSKLEGTVHVNVQLIRKFMKN
                     YFFSPAAYPPVAPDFSLKDDMFLFNQGPTKGLGSVQFHDYKPVFEANQQLPNVAIFIK
                     QIELFRELLEKAGPDKAQDMDPLFSLPLGEMFSIVVYGQLILEQAKFDQVDPDIVNQI
                     FDFMVRDFARFALQIYGMHNTKDEQRAYCKEIMLIKAQGDDVQYERVWKEYVYALNGE
                     YAMNE"
     gene            complement(22780..23604)
                     /locus_tag="PLA58_00455"
     CDS             complement(22780..23604)
                     /locus_tag="PLA58_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOR61293.1"
                     /translation="MESQIPKAIQLQYQPVAIFWSDEKPEGAMQFSEGKWGCVMWLAV
                     SAAKGRPAVADRKTFGCVGGGVGLGFGNQYLNFPGGMEGFCRFLSSGNALRPGGPELA
                     EKIKPFMTKESYENFLQGERYIKNPELAEKFVEALPMTDVPAAYVVFQPLSSVDPATE
                     KPQTILLFVNPDQLSALTVLANYARGDNENVIMPYAAGCQNIGIYPYREARSDRPRAV
                     VGLTDLSARVNVRKQLRDGNFMTFAAPFALFEEMERNIPGSFLERHTWQSLLPKEP"
     gene            24015..25550
                     /locus_tag="PLA58_00460"
     CDS             24015..25550
                     /locus_tag="PLA58_00460"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOR61294.1"
                     /translation="MNRAFANIREMVETQGRRCQDRIAIRFYDRDITYRDLDDESNRI
                     ANGLLKLGVGKGDRVCLLMDNSPEFYYVYFGIIKIGGIAGPVNCWWQAAEIRYLLQDS
                     GAVALFCDHNYRGLVDQIIGETPDMQHVIEREAADDRFLPFEKMLTEPVSLADAAISP
                     DDVSTIVYTSGTTGNPKGVLLTHRNILTNSWQASLLANIDENDVILCFLPLFHVNGLV
                     ITGTSPLSKGSQIVLRRNFSASEFWECVAKYRVSIFSGVPTVYQILLNTPGFEGLDVS
                     SLRYGVCGAAPMPVETIRKFEETFHMIIIEGYGLTESTAGATANPINGVRKIGSIGIP
                     FAGTEIRILDDADQEVPQGEVGEICIRGDHVMKGYFNKPEDTAQTLRGGWLHTGDMAY
                     KDEDGYLFIVDRKKEMIIRGGENIYPKELEGIICRHPDVAEVAVVGIPDAIYGEEVMA
                     CLVLHPDRTLTEEAFQDWCRANMASYKVPKYVNIRASLPRNILGKLLKKELKAALKSE
                     GKI"
     gene            25608..26834
                     /locus_tag="PLA58_00465"
     CDS             25608..26834
                     /locus_tag="PLA58_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOR61295.1"
                     /translation="MKISSIELYHVQVPLKQTFWPTWIPGYPQTHNGFTLIKLVTDDG
                     IEGYSAGSALGLERQGLGDLIGGYLLGADPTDIESVQALLKQAGILGWRNFWIEPACW
                     DIIGKSKGKPVYELLGGEKRPIEVYCSTGEMHEPEKRVEELLAMKERGFKTAKLRVKN
                     KDLKDDIRHIEVVRKGIGDRLVLGVDANQGWLVTIVDKIPAWDLKRATAFAEVCAANG
                     ISWLEEPLDSRDYDGNAALKKVSKVRISGAELNYGWDEIKIMFEKDCFHIYQPDATFA
                     GGIAQVKKVIDASHAHQREYSPHTWTNGIGFYVNWNLVLADKGNALPLEYPLEEPSWA
                     PEQREGIIDPILPDQNAMLAPFTKPGLGFEINQSLLNKYGKRFFKLTELRQKIKVIRQ
                     KGLKTALALKKRKESQ"
     gene            complement(26910..27563)
                     /locus_tag="PLA58_00470"
     CDS             complement(26910..27563)
                     /locus_tag="PLA58_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOR61296.1"
                     /translation="MKIFAVSDIHGATRSIEKAAHLIREADAVVISGDITHTKTRTEA
                     KDVLGCLEQHSKRILAVHGNWDREEVKDVLEEKGFSLHGRGRILDGTGFFGVGGSSPT
                     PIPTATVYSEEEIARILKTGYEQVRQASRLVLISHAPPRSVRDRSFIGLRGGSHSVKA
                     FLEAHPVGLCLCGHIHEAHGIERFQNTLVVNTGSFKKGRYAIIELGANPAVTPGKLG"
     gene            complement(27653..29407)
                     /locus_tag="PLA58_00475"
     CDS             complement(27653..29407)
                     /locus_tag="PLA58_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR61297.1"
                     /translation="MKRNLFLKILFSYLVIISVSFFILHLLVKDRISGIMTAGIEQEL
                     MHYAELVDLNAPQAMSAHLQQISKISKSRVTLVNERGVVFADSEKAIAGLENHLFRPE
                     IQEARLRGKGKAVRYSSSLGVNMLYVAVAIQDGKNSRGYVRLARPLHDVQSAVDQVYQ
                     SIFLAMLIGALVSLAVALFFSHRLAAPIRMMEEFTQRLRRGQPVGSIMLRTSDETRTL
                     ADNINYLVEELRDKIRLANEEKSKLMTALTSINEGVVILNRDERIEFLSPLLAGILSG
                     QYEDFLGKTLLEAFRNAELQKAFQSFKQTRQSILREIALGAAETVIMNVSLSEVQGLP
                     GEEKVMIVFHDVTRLKKLERVRTDFVANVTHEIRTPLTAVIGYLETLQAGALENPGDA
                     KRFTDIMLKQARRLHRLVEDLMILSRIELGEVVFRFEAVFLPEAMDSVISLLDAKAAA
                     KTIAMQNRLPQNLRPIKADRDRLSQILVNVLDNAVKFTPEGGRITMEAEERGDQVVLK
                     ISDTGIGIPVEEVQRLGERFYRVDKARSRELGGTGLGLSIVKHLMLAHGGRMEIESRP
                     GRGTTVSLCFPLNGPVKA"
     gene            complement(29417..30103)
                     /gene="phoU"
                     /locus_tag="PLA58_00480"
     CDS             complement(29417..30103)
                     /gene="phoU"
                     /locus_tag="PLA58_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOR61298.1"
                     /translation="MEEKRHTSLHYEKELQTIKDGLIYLGVLTEAAIGKAMKSLSGRD
                     TELAHQVVREDDEIDRLDTDLEERCIKILALRQPAAIDLRFITTAIKITGHLERIGDM
                     AVNIAEKVMRLNEEPQLKPYIDLPRMSELVGNMIRDSLDSFINSDLEKAEQVRRLEQV
                     IDDLNEQIFRELLTFMMEDSKNIHRALLVMQISKNMERIADHAKGIADMVTYMVTGNC
                     VRHQSCGMTT"
     gene            complement(30124..30882)
                     /gene="pstB"
                     /locus_tag="PLA58_00485"
     CDS             complement(30124..30882)
                     /gene="pstB"
                     /locus_tag="PLA58_00485"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017789372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOR61299.1"
                     /translation="MEAGDIISVNRVNFYYGAVRALTDISMTFKKNKVTALIGPSGCG
                     KSTLLRLLNRMNELIDATKVEGEILFEGRNIYAPDVDPVEIRRKIGMVFQKPNPFPKT
                     IHNNISYGPKLTGTSVADMDALVEQSLKQAVLWDEVKDILNKSAMNLSGGQQQRLCIA
                     RALAMKPDILLMDEPTSALDPISTAKIEELIEELKENYTIIIVTHNMQQAARVSDETA
                     FFYLGNLIEYNKTEKIFTKPDVKQTEDYITGRFG"
     gene            complement(30885..31763)
                     /gene="pstA"
                     /locus_tag="PLA58_00490"
     CDS             complement(30885..31763)
                     /gene="pstA"
                     /locus_tag="PLA58_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOR61300.1"
                     /translation="MKNLKNHSMRWRYFKQNVFFVLVRASALVITLALGGILLYILAN
                     GISVISWDFITKPPTDSMTKGGIMPAILGTIYLTIGAIAIGLPLGIASAVYLTEYARQ
                     GRVIRLIKVGINCLAGVPSVVFGLFGLGFFVIFLKFGSSILSGSLTLGFLILPTIIGA
                     SEEALKAVPQTFREASLSLGVSKWQTIYRIVLPNALPGILTGSILGIGRAAGETAPIM
                     FTAAAYFTAKLPGSVFDEVMALPYHIYVLATAGTNIEATRPIQFGTVLVLVAVVLGID
                     LIAILIRGSMRRNKRW"
     gene            complement(31773..32660)
                     /gene="pstC"
                     /locus_tag="PLA58_00495"
     CDS             complement(31773..32660)
                     /gene="pstC"
                     /locus_tag="PLA58_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOR61301.1"
                     /translation="MTRQTKEIIIQYIFLLFALVSVLVLGLIVFSLFREGLPLFTSVS
                     VKDFLFGKEWYPTYDPPSFGIFPLVVGSLIVTLIATLIAVPLGVMAAIYISEIAPQRI
                     KEILKSVIELLAGLPSVVLGFFGMVIVAPWLQETFDLPTGLNIINASVILAIMAIPTI
                     SSISEDALYAVPREFKEASYALGATKFETIAQVIVPAALSGISTAVMLGMARAVGETM
                     VVLMVAGGAAAIPESLFDPVRPMPASIAAEMGETPYGSAHYQALFAIGMVLFFLTLAF
                     NLIADYVSHKFRQTGSATL"
     gene            complement(32721..33137)
                     /locus_tag="PLA58_00500"
     CDS             complement(32721..33137)
                     /locus_tag="PLA58_00500"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOR61302.1"
                     /translation="MNFPKQTVLFICTHNAVRSQMAEALLNNIYGDRYAAYSAGSDPS
                     QIDPLVKTVMSETNIDVSHHRSKGFCVFQEVPLDYVITLCDQAKEACPYFPGGRFRMH
                     KGFSDPSRFQGSAEDLIQEYRRVRDEIKRWIEKEFH"
     gene            complement(33158..34009)
                     /locus_tag="PLA58_00505"
     CDS             complement(33158..34009)
                     /locus_tag="PLA58_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOR61303.1"
                     /translation="MFSIFQKAALLSIAFLMAAGVSFAADSIVIKGSTTVLPVAQGTL
                     EAFMKANPGVQMSLSGGGSGEGIKALIDKTTDIATSSREIKDKEIELAKSKGVTPVAH
                     VVAYDAIIPVVHPKNKVKNLSIDQLSQIYQGKITNWKEVGGDDLKIVVISRDSSSGTF
                     ESWDHFVMKKAKVTPKAQMLASNGALVTAVSKNKYAIAYLGIGYVNKSVKPLQVNGIT
                     ASLQTAMSKAYPFSRELYMYTDGEPHGSIAKYIAFVKSAEGQKIVAREGFVPLMDAKK
                     VSGRGKK"
     gene            complement(34136..34834)
                     /locus_tag="PLA58_00510"
     CDS             complement(34136..34834)
                     /locus_tag="PLA58_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOR61304.1"
                     /translation="MTKILIVDDEKDIADLISYNLEREGFRARIVLDGNGALAAVRSF
                     TPDLMILDLMLPGVGGLDICKRIRANPETSSLPIIMLTAKADEVDKIIGLEIGADDYV
                     TKPFSVKELVARVRTVLRRAQEGKKPSAKERFEFQGLSIDYGSCRVRVEGRPVTLSPT
                     ELKLLFFLSRNPGRVYSRDQILTQVWGDDTFITDRAVDVHIRRLRSVIEENMEKPQYI
                     LTVRGFGYKFADIR"
     gene            complement(34941..36302)
                     /locus_tag="PLA58_00515"
     CDS             complement(34941..36302)
                     /locus_tag="PLA58_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61305.1"
                     /translation="MKRFWFVLLSLGLMVAFSTSAMAVDVKVSGEYYAAGMYLDKTNL
                     TKTTTGGDPSTAFYYQRLRVRTDFTISPGLTLITRLDAMERAWGATRSATGTALAADS
                     AGTRAENENIAVDWVYIHYASPIGIFDVGYMNDGSTGTIFGNSYSPKARIKYSNVFGP
                     ITLNLAYTKEAENSRTAINAVNRADADNDKYGIEGVYNWKTGKAGVNINYYHQAANRP
                     AAPAYKRTYFLVTPYAFAKIGPVSLQAELNYLTGSDKYEDGTNPAGAPGIDKVDLAGI
                     SGWIDATANFGIFYAGGSIAYVSGNDPGTNDKIEGGIINGGRDWNPCLIMWNYDRTNW
                     AGALTGYNGAAQDTAMANGWLFQMRGGVKPVEKLDVLASVTYAFADQKPWGTAGNPAT
                     VYLHNDYGWEVDLTATYKITNNLSYMLGGGYLFTGKYYKGTSDANELTNDFLVINKLT
                     LTF"
     assembly_gap    36433..36532
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(36539..36799)
                     /locus_tag="PLA58_00520"
     CDS             complement(36539..36799)
                     /locus_tag="PLA58_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61306.1"
                     /translation="MAEKKIGEVLKFFAKPSVAAVHLSEGELSIGDSIKFSGHTTEFT
                     DVIQSMEVDNKPVQKAVAGDSIGLKVSDRVRPGDEVFKVIPD"
     gene            complement(36910..37317)
                     /gene="rplQ"
                     /locus_tag="PLA58_00525"
     CDS             complement(36910..37317)
                     /gene="rplQ"
                     /locus_tag="PLA58_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOR61307.1"
                     /translation="MYHGKAGRKLGRTSSHREAMFRNMVTSIIKHESIRTTDATAKEV
                     RKLADRMITLGKRGDLHARRQALSVVRDKEMVAKLFGELAERFRNRAGGYTRIVKVGY
                     RFGDNAPVSILEYIPDENRKEKAKPKKKEKKAE"
     gene            complement(37404..38378)
                     /locus_tag="PLA58_00530"
     CDS             complement(37404..38378)
                     /locus_tag="PLA58_00530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOR61308.1"
                     /translation="MQRNWSSLIRPKRVDVDEATHTRFYGEFTIQPLERGFGITLGNA
                     LRRVLLSSIQGAAITSVKIDGVLHEFSTIPGVKEDVTDVILNLKGVRFKLGAADTKVV
                     KFNVTSAGVITAGDIITDGTIEVLNPDHYIATLSGGGSLKAEMVVKMGKGYIAAKKEL
                     EPDQPEGTINIDAIFSPIKKVNYVVSHARVGQITDYDKLVLEVWTDGSLNPGDAIAYS
                     AKILKQQLDVFINFEEVEEDAAPEQGEDGKGNVNEILMRSVEDLELSVRSANCLKNAG
                     INTIGELVQKTEAEMLKTKNFGRKSLSEIKDILNEYGLAFGMKPEENA"
     gene            complement(38423..39052)
                     /gene="rpsD"
                     /locus_tag="PLA58_00535"
     CDS             complement(38423..39052)
                     /gene="rpsD"
                     /locus_tag="PLA58_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOR61309.1"
                     /translation="MARYTDSSCRLCRREGLKLFLKGDRCYSEKCSFERRGYAPGDHG
                     QSHKKQQSDYGTQLREKQKLKRMYGLLEKQFHGYFEKADRQKGITGTNLLVLLERRLD
                     NMIFRLGFANSRIEARQLISHGHFLVNGKAVNIPSYLLKAGDEVTVKEGSRKINRILE
                     AMETVARRGVPHWLGLDKDNFKATIKLLPVREDLTMPVQEQLVVELYSK"
     gene            complement(39098..39490)
                     /gene="rpsK"
                     /locus_tag="PLA58_00540"
     CDS             complement(39098..39490)
                     /gene="rpsK"
                     /locus_tag="PLA58_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOR61310.1"
                     /translation="MAKTVRKTGKRKEKKNITEGVAHIQSTFNNTIVTITDMTGNVIS
                     WSSAGLQGFKGSRKSTPFAAQMAAEDAVRKAKDQGMRRVQVYIKGPGAGRESALRSLQ
                     IAGLTITMIRDVTPVPHNGCRPPKRRRV"
     gene            complement(39518..39886)
                     /gene="rpsM"
                     /locus_tag="PLA58_00545"
     CDS             complement(39518..39886)
                     /gene="rpsM"
                     /locus_tag="PLA58_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOR61311.1"
                     /translation="MARISGVDLPKNKRMEVALTYIYGIGKTRAKQILKESGVSPDTK
                     TDQLADSEISAIRSIIDKEGKVEGDLRREISMSIKRLMDIGAYRGLRHRKGLPVRGQR
                     THTNGRTRKGPRRAIAGKKK"
     gene            complement(39912..40025)
                     /gene="rpmJ"
                     /locus_tag="PLA58_00550"
     CDS             complement(39912..40025)
                     /gene="rpmJ"
                     /locus_tag="PLA58_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOR61312.1"
                     /translation="MKVRSSVKKICEKCKIVKRKGVLRVICENPKHKQRQG"
     gene            complement(40060..40278)
                     /gene="infA"
                     /locus_tag="PLA58_00555"
     CDS             complement(40060..40278)
                     /gene="infA"
                     /locus_tag="PLA58_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOR61313.1"
                     /translation="MAKEEAIEVEGRVLEPLPNAMFRVELENGHKVLAHISGKMRMHF
                     IKILPGDKVTVELSPYDLTRGRITYRTK"
     gene            complement(40310..41059)
                     /gene="map"
                     /locus_tag="PLA58_00560"
     CDS             complement(40310..41059)
                     /gene="map"
                     /locus_tag="PLA58_00560"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOR61314.1"
                     /translation="MVILKQPDEIDKAKASSRIVAEVLSVLRERIKPGVTTRELDKIA
                     ESVTEKRGAKPAFKGYRGYPYSLCASVNEEVVHGMPSGRVLKEGDIVGLDFGVYYQGF
                     YGDAAVTLPVGRVSEEAARLMRVTEQSLYAAIEQASSGNRLGDISAAVQETAESAGYS
                     VVRDFVGHGIGRNMHEDPQIPNFGKKGRGIELQTGMILAIEPMVNAGRYRVKILPDGW
                     TVITEDGSLSAHFEHSVAITDNGPEILSRLN"
     gene            complement(41084..42391)
                     /gene="secY"
                     /locus_tag="PLA58_00565"
     CDS             complement(41084..42391)
                     /gene="secY"
                     /locus_tag="PLA58_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOR61315.1"
                     /translation="MLEGLGGVSKIPELQRRILFTLLLLAVYRVGVFIPTPGIDNTAL
                     LAFFEQARGSMLGLMDLFAGGALSTFSIFALGIMPYITSSIIINLLTVAIPHLEKLSK
                     EGENGRRKITQYTRYGTVGLSIIQGFGIAWGLQQMASPTGAPIVLHAGWGFILMTIIT
                     LTSGTAFLMWLGETITEKGIGNGISLIIFAGIVCNLPGAIGKTWRLYATGELHFLLLV
                     LLGLFMIAVIGAIVFVEAAQRRIPVQYAKRIVGRKMYGGQTTHLPLKINSAGVIPPIF
                     ASSVIMFPATIANFAPAGLMKTISGYLMPGRLGYEILFVGLIFFFCFFYTAVTIKPDD
                     MAENMKKFGGYVPGIRPGKKTAEYIEEILERLTFSGAVYVSIICVLPSVLISQLNTPF
                     YFGGTSLLIVVGVAMDTASQIESHLLTRQYEGFMKKGHMARRR"
     gene            complement(42393..42839)
                     /gene="rplO"
                     /locus_tag="PLA58_00570"
     CDS             complement(42393..42839)
                     /gene="rplO"
                     /locus_tag="PLA58_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOR61316.1"
                     /translation="MDLSSLKAPLGATKKRKRVGRGDASGHGGTSGKGHKGQKARSGG
                     YVRDGFEGGQMPMSRRLPKRGFRNPFRESYVGVNVMDLCRKFEKGAVIDEEAILKSGL
                     VKKKGAGIKLLAKGNIDFPVTVKMAKISAAAKAKITAAGGSIEEVI"
     gene            complement(42844..43032)
                     /gene="rpmD"
                     /locus_tag="PLA58_00575"
     CDS             complement(42844..43032)
                     /gene="rpmD"
                     /locus_tag="PLA58_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOR61317.1"
                     /translation="MGKMLKVKMVKSVITRPEKQRKIMRGLGLTKVNKTVTLADTPEI
                     RGMIHKVIHLVSVEESKE"
     gene            complement(43056..43520)
                     /gene="rpsE"
                     /locus_tag="PLA58_00580"
     CDS             complement(43056..43520)
                     /gene="rpsE"
                     /locus_tag="PLA58_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOR61318.1"
                     /translation="MKEVELLDRVIAINRTAKVVKGGRRFRFSAIVVVGDGQGMIGAG
                     LGKAHEVPEAIRKGMEMAKKNMVKVAVANKTIPYEVLGKFGAGKVLLKPASEGTGLIA
                     GGAVRAVLEVAGVHNILTKCLGSHNPHNLVKATVDGLCQLKDPAEISARRGK"
     gene            complement(43575..43940)
                     /gene="rplR"
                     /locus_tag="PLA58_00585"
     CDS             complement(43575..43940)
                     /gene="rplR"
                     /locus_tag="PLA58_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOR61319.1"
                     /translation="MASKKTLKVRAKREKRVRGKLTGTAERPRLSVFRSDKHIYAQVI
                     NDAQGTTIATASTLSRELAGKHKGIKKVDVAREVGKLIAARLQTLGVEKVVFDRGRFL
                     YHGRVKALADAARESGLKF"
     gene            complement(43977..44519)
                     /gene="rplF"
                     /locus_tag="PLA58_00590"
     CDS             complement(43977..44519)
                     /gene="rplF"
                     /locus_tag="PLA58_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOR61320.1"
                     /translation="MSRIGKKPIAFPKNVKISQNGNIVKVEGPKGTLSSKLPDGIAMT
                     MEENQLIIERKSDDRLARSYHGLARTLVNNMVTGVSAGFEKGLEISGVGYRAELAGSN
                     LKLVLGFSSPVEYSIPKGIEVRIDKQVNLVVSGIDKELVGRVASEIRSLKKPEPYKGK
                     GIKYAGEHIRRKAGKAAGAK"
     gene            complement(44554..44955)
                     /gene="rpsH"
                     /locus_tag="PLA58_00595"
     CDS             complement(44554..44955)
                     /gene="rpsH"
                     /locus_tag="PLA58_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOR61321.1"
                     /translation="MGMTDPIADMLTRIRNGNKARFKNVSVYMSQMNMNIAKVLKAAG
                     YIHNYEAVKDESGRAMLKITLKYPDAKHTVITDIQRVSKPGRRVYVSADKIPKVLNGF
                     GISILSTSRGIMTDQEARESNVGGEVLCKVW"
     gene            complement(44978..45163)
                     /locus_tag="PLA58_00600"
     CDS             complement(44978..45163)
                     /locus_tag="PLA58_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOR61322.1"
                     /translation="MAKKSLIAKAKRKPKFAVREYNRCQICGRPRAYYRKFEMCRICL
                     RKLSLQGELPGVIKSSW"
     gene            complement(45191..45730)
                     /gene="rplE"
                     /locus_tag="PLA58_00605"
     CDS             complement(45191..45730)
                     /gene="rplE"
                     /locus_tag="PLA58_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOR61323.1"
                     /translation="MARLKEYYLKTVVPALVREFDYKNPMQVPKMEKIVVNMGLGEAI
                     SNVKIIDGAVQEMAMITGQKPVVTKAKKSIATFKLRQGMPIGCSVTLRKDVMYEFFDR
                     LVNVALPKVRDFRGISASAFDGRGNYSIGLHEQIIFPEIEYDKIEKIKGMNITIVTSA
                     RTDEEARVLLRLMGVPFKN"
     gene            complement(45736..46059)
                     /gene="rplX"
                     /locus_tag="PLA58_00610"
     CDS             complement(45736..46059)
                     /gene="rplX"
                     /locus_tag="PLA58_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOR61324.1"
                     /translation="MSLGRLKKGDLVKVLAGKDKGKTGKILKAIPGKNKVIVEKINVI
                     KRHKRPDQKSKGGVLEKEAAMDVSKVSIICGKCKEATRIRNRILEDGKKIRICSKCND
                     VIDVG"
     gene            complement(46082..46450)
                     /gene="rplN"
                     /locus_tag="PLA58_00615"
     CDS             complement(46082..46450)
                     /gene="rplN"
                     /locus_tag="PLA58_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOR61325.1"
                     /translation="MIQMQTILNVADNSGAKRVACIKVLGGSKRRFASLGDVIVVAVK
                     EAIPNSKVKKGDVMKAVIVRTVKEVRRPDGSYLKFDDNSAVLISNQMEPIGTRIFGPV
                     ARELRAKQFMKIISLAPEVL"
     gene            complement(46499..46756)
                     /gene="rpsQ"
                     /locus_tag="PLA58_00620"
     CDS             complement(46499..46756)
                     /gene="rpsQ"
                     /locus_tag="PLA58_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOR61326.1"
                     /translation="MAEKSNKRTIKGVVVSDKMDKTIVVKAERLVKHPVFHKYVRRHV
                     KYKAHDEQNQCRTGDTVIIIESRPISRDKRWRMLEVLEKAK"
     gene            complement(46758..46952)
                     /gene="rpmC"
                     /locus_tag="PLA58_00625"
     CDS             complement(46758..46952)
                     /gene="rpmC"
                     /locus_tag="PLA58_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOR61327.1"
                     /translation="MKISEIRGLGVAELQTKKSELAEELFRLKIRHAGGQLESTASLG
                     RLRRDIARIKTVMKEKEAAG"
     gene            complement(46942..47364)
                     /gene="rplP"
                     /locus_tag="PLA58_00630"
     CDS             complement(46942..47364)
                     /gene="rplP"
                     /locus_tag="PLA58_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOR61328.1"
                     /translation="MLMPKRVKYRKLQKGRMGGKAIRGGSIAFGDYGLQAAECGWVSA
                     RQIEAARVAMTRYVKRGGKIWIRVFPHKSITKKPAETRMGKGKGAPEGWVAVVKPGSV
                     LYEMEGVTKEVAREAFRLASHKLAISTKFCSREISDEN"
     gene            complement(47404..48051)
                     /gene="rpsC"
                     /locus_tag="PLA58_00635"
     CDS             complement(47404..48051)
                     /gene="rpsC"
                     /locus_tag="PLA58_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOR61329.1"
                     /translation="MGQKVNPVGLRLGYIKKWQSVWFAEKNYSGMLLEDLKIRKYLKK
                     KLYHAGISRIEIERAANKAKVNIYAARPGVIIGKKGSEIEKLKAEIEKFSQAEIIINI
                     LEVRKPETDAQLVAENVAMQLERRVAFRRAMKKCVGYALKFGAKGIKISCSGRLGGAE
                     IARAEWYREGRVPLHTLRADIDYGFIEAHTAYGLVGVKVLIFHGEVLPQKNERQS"
     gene            complement(48064..48396)
                     /gene="rplV"
                     /locus_tag="PLA58_00640"
     CDS             complement(48064..48396)
                     /gene="rplV"
                     /locus_tag="PLA58_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOR61330.1"
                     /translation="MEAKAVAKYIRMSPQKVRLVVDLVRGKKVQEARNILLYTRKYSA
                     GIVAKVLKSAVANAAQNPNIDENTLYVKEIFVDQGPSLKRWRARAQGRAAGIRKRTSH
                     ITVVVDER"
     gene            complement(48436..48717)
                     /gene="rpsS"
                     /locus_tag="PLA58_00645"
     CDS             complement(48436..48717)
                     /gene="rpsS"
                     /locus_tag="PLA58_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOR61331.1"
                     /translation="MARSIKKGPYIEESLLTKVRKMEAESSKNVIKTWSRRSTITPEL
                     IGYTFAVHNGKKFIPVYVTENMVGHKMGEFAPTRTFYSHSGDRKTKVKK"
     gene            complement(48721..49545)
                     /gene="rplB"
                     /locus_tag="PLA58_00650"
     CDS             complement(48721..49545)
                     /gene="rplB"
                     /locus_tag="PLA58_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOR61332.1"
                     /translation="MAIIKYKPTSPGRRFQSVSDFAEITSTEPVKSLLKPIKRTGGRN
                     SYGRITARHIGGGHKRKYRMIDFCRNKADIPAKVAAIEYDPNRSARIALLNYRDGEKR
                     YIVAPAQIHVGDVLVSGEKADIKPGNAIPLKYIPLGSLIHNVELKVGRGGQLIRSAGA
                     YGQLMAKEGSYAQVRLPSGEVRKVFIECMATIGQVGNNEHENVSIGKAGRTRWLGKRP
                     HVRGVVMNPVDHPMGGGEGRSSGGRHPCTPWGVPTKGHKTRKNKKTDKYIVKRRGK"
     gene            complement(49567..49854)
                     /gene="rplW"
                     /locus_tag="PLA58_00655"
     CDS             complement(49567..49854)
                     /gene="rplW"
                     /locus_tag="PLA58_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOR61333.1"
                     /translation="MELHQVIRKMLLTEKSNLDREAANKYHFEVDRRANKVEIGQAVE
                     KLFKVKVTEVRVIHVLGKKKRMGRVMGKKSDWKKAIVTLAEGSRIEVAEGV"
     gene            complement(49854..50477)
                     /gene="rplD"
                     /locus_tag="PLA58_00660"
     CDS             complement(49854..50477)
                     /gene="rplD"
                     /locus_tag="PLA58_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOR61334.1"
                     /translation="MAVADVFDIEKKKVAEVELNDAVFGADINEATIYEVVKMQRASR
                     RSGTSATKGRNEVSGGGKKPWRQKGTGRARAGTSRSPIWRGGGIVFGPQPRDYAYSVP
                     KKVRKNALISALSMKVKEEKMTILRDFPMEAISTRAFQKVVDLFGLKKALFVIDQDNV
                     VLMKSSRNIKNVKMIRSEGINVYDVLNYEHLVLLEPSIKKIEGALLA"
     gene            complement(50496..51128)
                     /gene="rplC"
                     /locus_tag="PLA58_00665"
     CDS             complement(50496..51128)
                     /gene="rplC"
                     /locus_tag="PLA58_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOR61335.1"
                     /translation="MNKGILGKKLGMTQVFAEDGAAVGVTAIEVEPSVVVQVKTREKD
                     GYDAVQIAYGRIKQKNVTKPLQGHLNKANKGYFRVLREVPAAAGAYEVGQEISADIFQ
                     AGGFVDVVGTSKGKGFQGVVKRHGFGGGRATHGSMFHRAPGSIGASADPSRVFKGTKM
                     GGQMGNVRKTVQNLQVWQVRSDRNLLLLKGSVPGGKNGYVFISQARKKSA"
     gene            complement(51225..51533)
                     /gene="rpsJ"
                     /locus_tag="PLA58_00670"
     CDS             complement(51225..51533)
                     /gene="rpsJ"
                     /locus_tag="PLA58_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOR61336.1"
                     /translation="MKEQKIRIRLKAYDYKLLDRSVEEIVETAKRTGARVAGPIPLPT
                     EINKYCVNRSPHVDKKSREQFEIRTHKRLIDIVEPTQATVDALMKLDLSAGVDVEIKA
                     "
     gene            complement(51555..52748)
                     /gene="tuf"
                     /locus_tag="PLA58_00675"
     CDS             complement(51555..52748)
                     /gene="tuf"
                     /locus_tag="PLA58_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOR61337.1"
                     /translation="MAKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKYLAKKGFAEF
                     RAFDSIDNAPEEKERGVTINISHVEYETEKRHYAHVDCPGHADYIKNMISGAAHMDGT
                     ILVVAASDGPMPQTREHILLARQVQVPYVVVFLNKVDLVDDPELLDLVELELRELLSQ
                     YEFPGDAIPIIRGSALKALEAEDMASPDVKCIEELMKAVDEYIPEPKRDTDKPFLMPV
                     GDVFTISGRGTVVTGRVDRGIIKVGEEVEIIGIRPTIKTVVTGVEMFRKTLDEGRAGD
                     DVGLLLRGIKREEVERGQVVAKPASITPHTKFEAAVYVLTKEEGGRHTPFFSGYRPQF
                     YFRTTDVTGVATLPEGVEMVMPGDNVKMTVELIMPIAMEEQLRFAIREGGRTVGAGVV
                     SKVIA"
     gene            complement(52806..53276)
                     /gene="rpsG"
                     /locus_tag="PLA58_00680"
     CDS             complement(52806..53276)
                     /gene="rpsG"
                     /locus_tag="PLA58_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOR61338.1"
                     /translation="MPRRREIEKREILPDPKYNNKLVAKFVNSLLKRGKKSVAESILY
                     GAFDIIEKRVKEQPVEFFEKAVNNVKPVIEVKSRRVGGSTYQVPTEVVPARRTALAIR
                     WLISNAQERTEKTMREKLAAELIDAANNRGGAIKKREAVHKMAEANKAFAHYKF"
     gene            complement(53295..53666)
                     /gene="rpsL"
                     /locus_tag="PLA58_00685"
     CDS             complement(53295..53666)
                     /gene="rpsL"
                     /locus_tag="PLA58_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOR61339.1"
                     /translation="MPTISQLVRKGRTQIKRKTNAPALAGSPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTSGYEVSSYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTL
                     DAVGVADRKQSRSKYGAKKPS"
     gene            complement(53772..57866)
                     /gene="rpoC"
                     /locus_tag="PLA58_00690"
     CDS             complement(53772..57866)
                     /gene="rpoC"
                     /locus_tag="PLA58_00690"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOR61340.1"
                     /translation="MEDVFSYFEKPKNPIRFNAMKISIASPEKILSWSNGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGRYKRMKHRGVVCEKCGVEVIQSKVRRERM
                     GHITLATPVAHIWFLKSLPSRIGNVMDLTLKELEKVLYFESWIVLDPKDTPLKKKELL
                     TDEEYIEAKEQYGENGFVAGIGAEAVRQLLEEIDLDKLYEELRAEVVTSISDTKKKKI
                     VKRLKVVEALRKSGNKPEWMVLTVLPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLQELNAPEIIIRNEKRMLQEAVDVLFDNGRRGRAITGTNKRPLRSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPDLRLHQCGLPKKMALELFKPFVYNRLLEKGYV
                     TTVKSAKKMVERETSEVWDAVDEVVREYPVMLNRAPTLHRLGMQAFEPVLIEGKAIRL
                     HPLVCTAFNADFDGDQMAVHVPLSIEAQTEARVLMMSTNNILSPANGKPIIIPSQDIV
                     LGIYYLTRAKKDVKGEGMIFSGPDEVRSALDAGAIDLHARIKVRIESELKETTAGRIV
                     LFEILPEEISFDAINKLMNKKELANLIDHCYRVCSDKTTVLLADRLKDLGFKYATISG
                     LSFAVGNMIIPENKKNIVAQADKDVLKIQKQYMDGLITDGERYNKVIDIWAQATEKIA
                     SEMLTGISTEEVAGADGKKRRIESFNPIYMMADSGARGSGQQLRQLAGMRGLMAKPSG
                     EIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDCIIT
                     EQDCETVDGLYVSALMEGGEIIETAGERVLGRVALQEIRDPFTDEVIVKANEAIDEAL
                     AEKIDRAGIERVNIRSVLTCRAKHGVCAMCYGRDLAHGHLVNIGEAVGIVAAQSIGEP
                     GTQLTMRTFHIGGTAKFDEHSTLEARHDGRVKFVNLNTAKTRENDYVVMNRHGEIHVL
                     DEQNRSRGKYPVPYGAHIKVGDESSVKRGQLIAEWDPFSIPILAEVDGTIKYGDIIEG
                     KTMQEQVDEVTGLSRKVIVEFKSGDFRPRVSIKDSKGKTAKIAGSNAAARYFLSVGVN
                     IVVSEGDQVRAGDILAKIPRETTKTKDITGGLPRVAELFEARKPKDFAVISEIEGTVT
                     FGKDAKGKRKIVVTPEIGEEKEYLIPKGKHISVQEGDRVAPGDALMDGVNNPHDLLAI
                     KGEKELARYLVDEVQEVYRLQGVKINDKHMEVIVRQMLRRVRITDPGDTTFIADEQVE
                     RYRFQEENDKVMAQGGKPAIGDPLLLGITKASLSTQSFISAASFQETTRVLTEASLSG
                     KTDYLRGLKENVIMGRLIPAGTGLVMYRKLGIKVEEEEAEAASE"
     gene            complement(57904..62019)
                     /gene="rpoB"
                     /locus_tag="PLA58_00695"
     CDS             complement(57904..62019)
                     /gene="rpoB"
                     /locus_tag="PLA58_00695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOR61341.1"
                     /translation="MGDFRKNFGRVKKVQDIPNLIEIQTRSYEKFLQADVPLAKRRNV
                     GLQGAFKSVFPISDFSGKCSLEFVSYKIGDVRYDMKECIQKGMTYAAPLKIVVRLMVF
                     DTDRLPDGRGNRETPETKPIRDIKEQEIYFGEIPLMTENGTFIVNGSERVIVSQLHRS
                     PGIFFDHDKGKTVASGKLIYSARVIPIRGSWLDLEFDSKDLLYVRIDRRRKMPVTILL
                     KAMGNSTEFLLNYFYHIEKITLEGEKIYFGVDESLAGQKAPEDIKDPKSDEVIVKKGR
                     KLTRPLLKKIMDAGIKRVAIKEADLTGKILSSDVQDPSTGEILFRCNEELPFNGLEIA
                     QEKGVRELNFIHIDEDLDNASIRDTLLMDRIDTAEDAIMEIYRRLRPSNPPTPETAKK
                     FFNSLFFELETYDLSDVGRAKMNYKLGLNVPTDLTVLRNEDILASVKYLIDLKNGVGE
                     CSVDDIDHLGNRRVRSVGELIENQYRIGLVRMERAIKEKMSLQDIETMMPHDLINAKP
                     VSAVVNEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHSTHY
                     GRICPIETPEGPNIGLIVSLSTYARVNEFGFIESPYRLVENGKVLNDVKYMTAIEEEN
                     QLIAPADRPLDKKGKFEEELISVRKGGDFVSAMPEDIKMMDVAPDQLVSVAAALIPFL
                     EHDDANRALMGSNMQRQAVPLMCPEVPVVGTGMEAVVARDSGAVIVSKRSGVVESVDA
                     SRIVVRADKPEEDGLDTGVDIYTLSKYQRSNQDTCFNQKPIISTGDRVRKRDILADGP
                     ATDTGELALGRNVMVAFMSWGGYNYEDSILVSERIVKEDIYTSIHIEEFETMARDTKL
                     GKEEITRDIPNVGEEALRNLDESGIIRLGVSVKPGDILVGKITPKGETQLSAEEKLLR
                     AIFGEKASDVRDTSLRVPPGVEGTVIDAKIFTRKGADKDLRSRAIEEEAIEKLTKDRD
                     DEIRILAESIKKDIAKLVVGKTAAGKIVDVKKKTLLKKGEKITREAWQEIPFMYWKDV
                     TLSEHDGSEEKLANLLSVMERKIDFVKARFEEKLEKIQASDELPPGVIKMVKVYVAIK
                     RKLSVGDKMAGRHGNKGVLSRILPEEDMPYFSDGSTVDIILNPLGVPSRMNVGQILET
                     HLGWAAKSIGEGLNELLEKNNNLNQIKSELRKTYASPDFDEFLEKASAEDIKRFVGRL
                     KKGMLVASPVFDGCPEPQIRKLLDTAGLPESGQAVLFDGRTGEPFDQEVTVGIIYMLK
                     LHHLVDNKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAMEAYGASYTLQEFLT
                     VKSDDVAGRTRIYESIVKGEHAFEPGLPESFNVLVKELQSLGLDVELVEEE"
     gene            complement(62056..62448)
                     /gene="rplL"
                     /locus_tag="PLA58_00700"
     CDS             complement(62056..62448)
                     /gene="rplL"
                     /locus_tag="PLA58_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOR61342.1"
                     /translation="MADQITKEDVVKFIENMTVLELSNLVKELEEKFGVSAAAPMMAA
                     VAMPGQAAGAAPAAEEKTEFNAVLTGFGAEKIQVIKVVRAITGLGLKEAKDLVEGVPK
                     PIKEAVSKEEAAEIKKKIEEVGGTVEIK"
     gene            complement(62474..63001)
                     /gene="rplJ"
                     /locus_tag="PLA58_00705"
     CDS             complement(62474..63001)
                     /gene="rplJ"
                     /locus_tag="PLA58_00705"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOR61343.1"
                     /translation="MDRRTKEQIVSELQEKLKKAHLGVLTSFNAMNVEKMEALRNALR
                     KNDAELKVVKNTLLGIASKETGFAVLADHFKYPIAVVLSYKDPVAPTKALMEFAKKNP
                     ELDIKVGVLDGRMLTKAELTALADLPGREVLLGKLVSVMAAVPTSLVTVLSAVPRSFV
                     QVLHAYSEKKQTLNS"
     gene            complement(63272..63973)
                     /gene="rplA"
                     /locus_tag="PLA58_00710"
     CDS             complement(63272..63973)
                     /gene="rplA"
                     /locus_tag="PLA58_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOR61344.1"
                     /translation="MLRRGKRIIAAKKKVDPTRRYTLKEATEMVVSMAGVKFDETVDA
                     AVRLGVNPAHADQMVRGSVVLPNGLGKTVRVLVFAKGDKEKEALEAGADLVGNDEIIE
                     KIKGGWLEFDRVIATPDMMGSVGKIAKILGPRGLMPNPKVGTVTFDIANAVRELKAGK
                     VEFRVEKAGIVHSPVGKVSFGAEKLYENITALLETIIKLKPASSKGTYIKSIAISSTM
                     GAGVKVDPLDIKSVS"
     gene            complement(63993..64418)
                     /gene="rplK"
                     /locus_tag="PLA58_00715"
     CDS             complement(63993..64418)
                     /gene="rplK"
                     /locus_tag="PLA58_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOR61345.1"
                     /translation="MAKKVIANIKLQIKAGKATPSPPIGPALGQHGVNIMEFCKAYNA
                     MTQNQEGMIVPVVITVYADRSFTFITKTPPASVLLKQAAKVAKGAADPKREKVGTVTA
                     KQVQDIAQLKFNDLNAVDMEGAVRIISGTARSMGIEIAG"
     gene            complement(64486..65019)
                     /gene="nusG"
                     /locus_tag="PLA58_00720"
     CDS             complement(64486..65019)
                     /gene="nusG"
                     /locus_tag="PLA58_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOR61346.1"
                     /translation="MAFKWYVVHTYSGFENKVKLSLQERIELAGAQAYFSDILIPEED
                     VVELISGEKKTSKRKFFPGYILVRMEMSDENWHIVKNTPKVTGFIGSKDKPSPIPDKD
                     VENLKVRIDEGTLKPKPKFKFDVGDHVRIIDGPFTNFDGVIDEVRPEKSKLRVIVSIF
                     GRPTPVELDFIQVTQGQ"
     gene            complement(65024..>65305)
                     /gene="secE"
                     /locus_tag="PLA58_00725"
     CDS             complement(65024..>65305)
                     /gene="secE"
                     /locus_tag="PLA58_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOR61347.1"
                     /translation="PDSKSGCWGFKSLLACQFFPKTKADDMEKFKLLFDRAVQFLAQA
                     KTELKKVTWPTRKQTLASTGVVMVIVAISSLYLGVIDLILAKLVKFILG"
BASE COUNT        14065 a        18831 c        16514 g        15795 t
ORIGIN      
        1 aaaatcatgc gaaaaaattt tgcttcccgg tttcccgatg gaacttcccg caactggcgg
       61 gattggcgct ggcagttgcg ccatcgcgca acatctctga tcgccttgac ccggattctg
      121 gaaacgccgc cgcgcgacgg gaaaccgctg gaacgcgtaa tgtcccgcta tcccatggcc
      181 attactccct actacctgtc tctcctggaa ccggaccatg aaaacgaccc cctgtctctc
      241 caatgcgtgc cggatctcag agagctgtcc ttctccgcgg gccttaggga cgacccgctc
      301 ggcgaagacc gctccatgcc tgcgccgaac ctgattcagc gctatccgga ccgcgtgctg
      361 gccatcgtca cacaccgttg cgccacctac tgccggcatt gcaaccgcaa acgtttctgg
      421 agcgtccctg aagccggtcc cttaaaaaac cgcctggcgc caatggtccg ctttgtatcg
      481 gaatcgccgc agattcgtga agtcatcctc tccggagggg acccgctgat gtttgacgac
      541 gcctccctgg aatggatcct gtcatcgttt gcgtccatac cgcacgtcga ggtgctgcgc
      601 atcggcagcc gcgcccccgt ggtcctgccg atgagaatca cgggaaatct ctgccggatt
      661 ctgaagcggc accggccgtt atggttcaat acccagttca atcatccttc ggaaatcacc
      721 aaagacgccc agcgggcgtg caacatgatc caggaagcgg gaattccggt ttccaaccag
      781 tcggttctgc tccagggcgt caacgactcg ccgaaggtta tgcaaagtct gctgaacgga
      841 ttgcagaaaa tatcggttcg cccttattat cttttccact gcgaacccgc gcggggctgc
      901 aatcatttcc gcacggacgc caggaagggc ctgaatctca tggaaaagct tcatcgaagc
      961 tgctcgggtc tggcgcttcc gcaatacgtg gccgatctgc ccgggcggga ggggaaagtc
     1021 cccctttggc ccctgtcgcg gcccatccaa aatgccttgc gaaaacatca agattttttt
     1081 gacaattttg aataattgga ttaggaagag cgcgaagata tcgcttcaga gccgtcaacg
     1141 aaggagaaag actttttatg tacaccgcca gtgatttgag aaaaggcctg cgcctgaaaa
     1201 tcgacaacga tccctacgtg atcaccgagt ttaactttgt caaaccagga aaaggacagg
     1261 cgctttaccg ctgcaaatta aaaaacatgc tcaccggaag ccagtttgaa cggaccttcc
     1321 gctccgtcga tgttttcgag cccgccgacc tgcgcgaaaa gaaaatgcaa tacctgtaca
     1381 aggaagggga caaattctgc ttcatggaca atgaaacctt cgagcaggtc tatctgaccc
     1441 aggaacaggt cggggacgcc gtaaacttcc tgctcgacaa cattgaagcg gaaatccttc
     1501 tctttgaaga ccgtcccatc ggcatcagcc tgcccaattt tgtcgaactg gtcgtgaccg
     1561 aagccgaacc gtgggccaag ggagatacgg tgtccggcgc gaccaaaccc gtcaaggtgc
     1621 agaccggata caccattctg gtgccgacgt tcatcagcga aggcgaaaaa atccgcatcg
     1681 acacgcgaac gggagaatat ctgacgcggg tcaaaggcta gcgcaagcaa ctttccaaaa
     1741 tgctcatatg ctgcgttgcg tttcagcttt cgtcgttgca acgcatacaa aaatacgcat
     1801 ccctcctcat gcttcacgcg ccttgcctct gaaacatttt gaaaagctgc ataaccatga
     1861 gcacactccc aaaggacgat tgtttctatc tggcgcgcaa ggcgcaggct ctgcaactgc
     1921 gcgccggatt taccgaacat cttcgccgct tctttattca acaaggtttt ctggaagtcg
     1981 aaacccccct gcgcatcccc gcgcccgcgc cggaagagca tattgaaccc cttccctccg
     2041 gaaactggtt tttgcagacc tcccccgaga tttgcatgaa aagacttctg gccgccggtt
     2101 acccccgaat cttccagttt tgcaaatgct tccgggccgg agaaagaggc aaccggcacc
     2161 tgccggagtt ttcgatgctc gagtggtacg ctctgcactg cgactaccgg aaactcatgg
     2221 accagtgcga agatcttctc atttccgcct gccggcagat gggccgttcc ggaaagatcg
     2281 tctggcagaa taaaactatc cgcctttcgc cgccatggga aagaatcacc gtggcggatg
     2341 ctttttcccg atatgcgccc gtcagcctgc cgaacgcgct ggccggcgac cgctttgacg
     2401 aggtattggt cgagcatgtc gaaccgaacc tcggcaatga ccttccaacg tttctttttg
     2461 attatccggc gcaaatggcc tccctggcca aaatcaaaaa agatgatccg gccgtcgcgg
     2521 agcggtttga gctttacatc ggcggcatgg aaatcgccaa cggcttttcg gaattgaccg
     2581 acgcccggga gcagagacgg cgctttgaag aagccctgaa ggcgcaggcg gccaggcatc
     2641 aggtccacta cgccatgccg gagccgtttc ttgcgtctct ggaaaacctg ccgccctgcg
     2701 ccggcatcgc gctgggtctg gaccggatga tcatgattct ggccgatacg gcaacgattg
     2761 atgatgtcat cgccttttcc cccgaaacac tttagccgca ggaaaaagat atattgacaa
     2821 gccttttttt ttcctttagg ttaccctgca aacacagaca aatcgatgag gcaagatagc
     2881 aaatgattct ggagatttag acatggaaag taattttcac ggttctctgc tgggcatgat
     2941 tctagacgca gggttgatgg ttaaatttgt gttgctgatc ctgctggttt tctccatcat
     3001 ttcctggacg attattttca taaaattccg aacctatcgc agaatccgtc aggaaaatga
     3061 ggccttcgat tccgattatc agaagagcac gaaactttcc gatcttcttc cgacctccag
     3121 gaagtatccc ttctccacca cggctgaagt tttccgcgcc ggctacgcgg aaatgaccaa
     3181 agcgaaccgg ctctcccggg attcggcgcg gcccgaggaa atcagcctgt cctctttgga
     3241 caatgtggag cgcgccctca accgcgcctc cagcaccgaa atgaccaagt tggagtccgc
     3301 gctgggattt ctggccacaa ccggttcggc cagccccttc atcggattgt tcggaaccgt
     3361 ctggggaatc atggaaacct tcaaatccat cggcgcgaga ggttcggcaa cgctggccgt
     3421 tgtcgccccc ggcatctcgg aagctctgat cgcaacggct gcaggactgg ccgcggccat
     3481 tcccgctgtt attttctaca actactttct gaacaaatcc agaacgatgg tccaggaaat
     3541 ggataatttc gcggatgaat ttctaaacat tgtcgaacgt tatctgatcc gtaaataaac
     3601 gatatcgggt gaaacagaat gcggcaggcg cgcagaagag aatctgaaaa gaaatccatg
     3661 gcggaaatca acgtcacgcc gctcgttgac gtgatgctgg tgctgctgat cattttcatg
     3721 gtaaccgcgc cgatgctttc catgggcatt gatgtcaatc tgccccgcgt taaatcaaaa
     3781 agcatcgacc ttgccgaaga aaagctgatc ctgagcatca atgagaaccg ggaaattttc
     3841 ctcaacaaaa ccaggctgcc ggttcaggat ctgaacgcaa agctggcggc catttttgcc
     3901 gggcgcgttg accgggaagt ctttctgcgc gccgacaaaa acgttcctta cggcttcgtg
     3961 gtggaagtca tgtctgaaat acgcaaagcc ggcgtcgata aactcggtat gatcaccgag
     4021 cctcccgggg attcaaaatg atgtcccgga tgaccggcaa aagggttttc ggcaaaaaca
     4081 gcggcgccgg caatatgttt ctggcctctc tgtttctgca cctgatggtc atcgccgccg
     4141 ttttcgcaac cgtgcccggc tcgtcaaagc agctgacttt cggagcgcct tattccgtgg
     4201 cgctggtcgg acccgacgtc cttgaatcca gtaaagagga tgtcccgaag ccaaaggatc
     4261 ttcttcaatc aggggcttcg accggccccg tcattctcaa gcgcgacatc ggagcctcgc
     4321 cgtcttcgtt cgtcatgaaa aaagacgaca gcggcaaacc ggacatagaa aaagcgattc
     4381 gcgctctaga gcagaaggaa acggccgcag ggcaaagcaa agattcaccg gcggcttcaa
     4441 cctcccccat agccgctgcg gccaattcat cacaactgaa cgaatacagc cgtcatgtct
     4501 ggtccaaagt aaaaagaaac tggtcgcttc ccgccgctct gatgcccaaa aacaacgtgg
     4561 aagccgtgat tgaagtgcga attgcaaaaa gcggcgcggt ggagcatatc ggctttgaaa
     4621 aacgctcggg caacagctac tttgatgaat ccgctctccg tgcggttaaa aaatcaaccc
     4681 ccttcccgcc gcttggggga tggacatccg gccgcacggt tgaaatcggc attcgtttcc
     4741 attccgcgga actgcgctga cgcatccgtc gtaaaggacc ctgttcatgg atagaaaata
     4801 tcgcctggcc gtaggaacgc ttcttgtttt ggcatggatt ttcaacgccg ccggcgcggc
     4861 agcggacaag gtttatatcg acatcgacgc gcctggattt cagcaatttc ccgttgccat
     4921 ttgcgatttc cagatcaaaa caggcgaccc ttcgaaagaa tctgtcgatc tttccgacag
     4981 cgtccggcgc tatctcgacc tgacgggaat ctttcgcgtc ctggataaaa aaagctttct
     5041 ggaaggcgtt gagccgggat cgccggaaat cattcgcttt tccgactggt cggtcatcgg
     5101 cgccgatttt ttgctgcggg gcaacctgac ggtcagggga aaggacgtgt tcgcggaaag
     5161 ccggctgtat gacgtcacgc gcggtcagat gcttttcgaa aagaaatatt caggcataac
     5221 cgacgatttg cagtccgtag cgctctccat cgcctcggac atccttctgg ccttgaccgg
     5281 tgatccgggg gattttcaca ccaaaatcgc ctttgtgatc aaaaaaaagt caagctccga
     5341 cattttcacc atgaactatg acgcctccga tgtgaaaaat ctgacgaacg accacagcat
     5401 tgtcgcggcg ccccgctggt cgccggatgg ccggtatttt gcctatacct cgtataagaa
     5461 cggccggccc atggtttttc tgcgccacct gaaaaccgga aaggaaaaaa ttcttgcttc
     5521 tttttcaggc ctcaatctgt gcggttcctt ctcgccggac agcaaaaaaa tattgctgac
     5581 gctgagcaag gacggcaatg aagaaattta cgtcctggac attgaaacga tgaacctccg
     5641 gcagctgacg cacagttttt ccattgatgt ttctcccgcc tggtcgccgg acggaaggaa
     5701 aatcgctttt gtatccaacc gctccggctc accgcaaatc ttcgtcatgg aagcggacgg
     5761 aaacaacgtg aaacggatca ccttcgaagg caactacaac acctctccct cctggtctcc
     5821 ccgcggagga agaatcgcct atgaaggact gatcaacagg aagtatcaga ttttctccgt
     5881 ggatgatgaa ggcaacaatc tgattcagct gacctttgat gacgcggaca acgaatatcc
     5941 ttcctggtct ccttccggca ggcagattgt attccattcc cggcaaaacg ccagaagccg
     6001 gatttgcatc atgaacgcga atggaatgaa cttccgtatt ctgaaggaaa gcggggactt
     6061 cctggtcatg ccggcatggt cccccagatc cgaacaaaag taattttcca taatttttat
     6121 tgacgtgaga cggcaagtta tgttttaatc ccggcgaatc caaaagacat tacaaaataa
     6181 cgaaaggaga acatcatgaa gaaacatttg acggtcatcg gattatttct gattcttgct
     6241 ttcagtttaa ccatattcag cggttgtgcg gaaaaaaagg ctgtggtcaa ggatgaagcg
     6301 attcaggaac agaaggtggc ggccgctcag gaagcggctc cggcagccgt aacggccgat
     6361 gatgaagccg ccaggaaagc gaaagaagcc gcggacaggg aagccgccct gaaagaacag
     6421 gccgccaaag atgaagccgc cagaagagaa gccgcccgcg cggcctgggc caagaagaac
     6481 gcggaagcgc tggtggaact gagcctgcag aatatttact tcgactacga caaatccgcc
     6541 attcgccccg acgcgcgcga aatcctgaaa gccaacgctg aaatcttcac caaaaacagc
     6601 tccgcgaaga tcgtcgtgga ggggcactgc gatgaaagag gaacggccga atataacatg
     6661 gccctgggag aaagacgcgc gcaggaagcc aagcagtacc tggtcaacct cggcattgac
     6721 gcttcccgga ttgaaaccat cagctacggc aaggagcgtc cgctggacaa ccgcagcacc
     6781 gaagaagcct gggcgcagaa cagacgggcg cagtttcttt taaaatagcc aggggctgcc
     6841 acaggagaca tttttgaaac gcttttggca tcttatactg ccggcgatgg ccttctccct
     6901 tgccgcgtgc gcgacctcct ctgatttaaa ggaactgcgc acggagctga accagcagat
     6961 ggaagcaaaa ttcgccgcgg cggacacccg gttggatgac cgggtatccg ccctgcaaaa
     7021 agcgcaggcg aaaaacgccg aagacctgtc ctctctgcgc aagacaaacg ccaacaccaa
     7081 cgccaatttc tcggacctgc gcgaccaggt gcagcaactg cgcggccagg ttgagaccct
     7141 gaagaaggaa accagccggg atcccaaaaa agacgcggaa atgcaggaaa agatcgacca
     7201 cctgctggtg aagatcaatt tcattgaaaa ctttctggaa atcggcaaaa aagacgcggc
     7261 aggcgcagcc gacagcgccg cttcaggcaa agaccaggga aagcagggca aagccgcggt
     7321 ctatgcggca gcctaccaga cttttaagaa cggccattac acgaaggcca gggaggcttt
     7381 tcaggacttc ctgacggcct acccgaccgg cgaatattcc gacaacgctc agttctggat
     7441 tggcgagtgc tatttttttg aaaaaaacta cgaaaaagcc attctggaat atgaaaaagt
     7501 aaccaaaaaa ttccccagca gcaacaaagt accctacgcg cttttaaagc agggcctctg
     7561 ctttcaaaac ctgggcgaca aggtcagcgc caaacttctt ttgcaacagg tcatcaggga
     7621 ctatcccaac acaagccagg cgaggattgc gcgctccaaa cttcaggaaa tcaaatagaa
     7681 aaaatcttgc tttgtcttct atccggcccg cttgatccgc cgctgcagtt tcaatccaaa
     7741 cgaaggatct tcgctccgtc cggggccggc agcgcaaaca ccgaaaaatc ggccgtttct
     7801 tcaatgttca catcggaata ttccacgaag gcggacaggg tgttgtccgc ccgttgaatg
     7861 aggatctttc gggccaggaa gagccccggc gtataatcct cataccgcac cgaatacact
     7921 tcccttccgt ccgaaccata ccggacaaac cttgtcaatc ttccgctgtt ctccatccag
     7981 atcgtttgcg aaggtcccga aaccgttttc atatccacgc gcagggcccc gccttccgcg
     8041 gaatccccgt aagaccaatg ggccccgtcg agcggtggaa aggagccgga aagaatcatg
     8101 accaggtctt caagattcaa tgtccagggt aaaaagcggg acatattttc agcggagggc
     8161 tgcccgacat aaaattcacc ctgcgacggg atccatattc tcatctcccg gggcgtcgcg
     8221 ctcaggaaaa aatcgggcgt gccgatgacc ggcagcattt ccaggcgaag ataggagggc
     8281 ctctgtagaa tcagagcagc ccggagggga taatgcccct gaaccgtgga cagatccatc
     8341 cgcgccgtag cgacgatcct gtgcgtttcg cgaagggcag ggcggagcat cgccgcggga
     8401 tccggatggc gtccggattc cgtaacggcc ccgccggcgc atccgcaagc cagaagcgac
     8461 aggagcaaaa tgcccgtcat ccttttgccg gaagcaaagt cgtaaaacat gcaaaacatc
     8521 ccgaatgtat ttgatgcgga aagcagcgct catcaaggtt tcagaaggtc tatcttgtcc
     8581 ttcaggcggc tgttgccagg atccactttg atggcctttc gataccattc cagcgcctct
     8641 tcccgacggc cgattttgag atacgtgtct cccaggtgct ccatgacgct gggatcttcc
     8701 ggcaggaacc ccagggcttt cttcaggtat ttcagggcgc tttcgtagtc gttttttttg
     8761 aaatacaccc agcccagcga gtccagaata tagccgttgt ccggtttgat cttcagcgcc
     8821 tgaacaatca tttgttcggc ttcgtccagg cgcatcccgc ggtcggcata gctgtacccg
     8881 ataaagttga gggcgtccgc attttgcgga tcaagcgcca gcacctgttt catggattga
     8941 atgctttcct cgaaacggcc cgttttttcc aggagcgccc ccagaagata atgcaggtcc
     9001 gtgttgccgg ggaaaagtcc ggtgccttcc cgaaccgtct tttcggccgc ggccatgtct
     9061 ttttgctctt catacagcga ggataaatac agatataata tggcctgatc ttttctttgg
     9121 acgatggccc ggcggaccac cgcgatcgcg cccgtgaaat ctccttcctg cttgatcagt
     9181 ttggccgcct gaatctgggc attggcatat aattcaaact tcggggagat cttctgatat
     9241 tcggtcagcg ctttttgcag atcgcctttt tgctccagga cactggccag aagataccgc
     9301 agtttgtcat cggcgggtgt tgtctccaaa agcagcgaga actgagcggc cgcctggtca
     9361 taccggcgga tatcgtaata taaaagcccc agggccattt gcacttcccg gttttcggga
     9421 ctgattttca gcacctcccg cagttctttt tccgccgctt cctcccggtc ggttttcatc
     9481 agcagctccg cgatcttcac acgaatgccg atccgggcgg gattcatctc cagatagcgc
     9541 cggtaaacga caatggcttc ttcatgcttg ttttgatttt cataaagagc ggcaaggccc
     9601 gtccaggccg cttcaaagga cggtcgctgc gcaataatct tccgatagag cttttccgca
     9661 tcctccgtcc gattcatctg cgtcagtgtt tttgcgtaat aaaacatccc gatgatatga
     9721 tcgggatcca gcgcaagcaa tttttgataa gccttctcgg cctggtcata tttcttctcc
     9781 tggctgtaaa tcgtcgccag ataaagatag gcgaccgcat tttccggatc catttccgtg
     9841 acggtctgat acgcccggac ggctttgtca tattcgcgca gattgaaata caggccgccc
     9901 agaatggacc ggagttcgat attgcctgga tctttcgcga gggcggactc tccgagagac
     9961 agggccggat cgacatcatt gttttcgacg tagagcgaaa ccagctcggt ggtcaaataa
    10021 gacgaatcgg gagaaaagga aagcgcgcgt ttcatttgat cgatcgcttc gtccagattt
    10081 ccgtccagcc ttgacagcac ggccagcgaa tagtggcagg cggcctcttt ggaatacgga
    10141 tccggaaccg gggcatccgc cggcggccgg cttgcgggaa aagaagcgca gccggcaaga
    10201 atcgcggcaa aaagaaatcc gcagatcagg aaaaaaacgt gctttttttg cataggagac
    10261 gcttgccttc gcttccggtt ttcgggaaca ccgtttttca ggaggcccct tgctttccaa
    10321 gcaatgccga taccggaaca atcgtgattc cctgttcccg gcacaccttg ccggcctccc
    10381 ggacggcctg aatggtttca ggatagggat gaccgatcac gatgagcggt gaatttccgt
    10441 ccgatgatcg ggcggcctcc ataagaatct gataaatttt ggaagtgtcg cgttcattgt
    10501 ccagaaagat ttttctggac atcaccgtca gacgggcttt gtgcgccgcc gcttccgtct
    10561 ttgaatcccg ggtggtccgg gaatcgataa aaaacaaatt tttcttttta agctcgcgaa
    10621 aaaccagcgt cagcttttcc tcgtccgcca tgaatttgga ccccatatgg ttgttgacgc
    10681 cggaggcata cggcacggag tccacgtttc tctgaagctg aaagaggatc tcttcatcgt
    10741 tcatatccgt aaaaagagcg ccgcggcccg gtttttcctt cggatacgaa agaggctcca
    10801 tgggcaaatg gagaaggatt tcacgatggg cccggtgaag cgtttccgcc gcctcgcgcg
    10861 aatgggcaag gagcggcaga acggcaaacg tgatattctc attgatggcc agcagttcgc
    10921 gcaccggctt catgtcatag ccgatgtcgt cgatgacaat cgccacctgc cttgccgccg
    10981 atcggggcgg cgcgatcctg gcaagctgcg cgccgtcttc cgttttgtgg cgctgcgcct
    11041 tcgccgcctc ccgcaacccc tccgcctgtt ctttcggcaa atgcggcggt ttgacgcccg
    11101 tcgtctcttc acgatcccac aggacaatgg cggcaatggc ggcaacggca aggacgatca
    11161 gcgtaatcag gacggttgtc gtcttctgga tctttttctt tttcatggcc gtcagtattg
    11221 gactcccgga accagcgacc ttatttggcc agatttttct tcatgatatc ccagcttttt
    11281 aaaatatcaa tcgccgcctt gagctgatta tccttcacca gatccgtcgt ttccctggaa
    11341 tctccgtttt tattttcatc gggatgcgcc ccctcttctt tggccggctt gatgtgcccc
    11401 ttgagatcct tttcccgaag cctttccccc atgagagact ccggttcctc cgagggccgc
    11461 accggcttga cgataatgtc cggcttgatg ccttcggcct gaatggaccg gccattgggc
    11521 gtatagtatc gggccgtcgt caatttcaag gcggagccgt cctccagggg aatcacggtc
    11581 tgcacggacg cttttccgaa ggtttgcgtc cccacaatca gcgcccggcc gttgtcctgc
    11641 agggcgcccg ccacaatctc cgccgcgctg gccgtgcctt cattcaccag gacgacaatg
    11701 ggcgcgttga cttccttgtc gccgctgtcg cgcgccgtgg ctttggtttc catctttttc
    11761 gtccggccgc gggtggacac aatcacgccc gacttcagaa acatgtcgga cacttctatg
    11821 gcctgggtca gcagtccgcc cggatcgttg cgcagatcca gcaccaggcc cttcatgggc
    11881 ttggtttttt cggccacgtc ggtaagcgct ttgcgcaggt catcggcggt tcgttcatgg
    11941 aaggaggcaa tccgaatgta gccgatatgg tcatcaaaag cctttgccct gacgcttttt
    12001 acctgaatca cggcgcgggt cagcgtgatg tccctcggct gggccatatt ctcccgcaga
    12061 atcgttatgg tcaccttggt gtctttcgga ccgcgcagct tcttcacggc ctccataatc
    12121 gtaatgtctt ttgtggattt accgtcaatc ttgacgatcg aatcgccggg tttcacgccc
    12181 gccttgaacg ccggtgtgtc ctcgatgggg gacactaccg tcagcacgtc cttcaggatg
    12241 gtaatttcaa tgccgattcc gccgaaacgc ccctgcgttt ccacttccag ctccttgtaa
    12301 agctcaggcg tcataaacga gctgtgcgga tcgagagact tgatcatgcc gttgatcgcg
    12361 ccctgaatca gaaccgaaga atcgatttct tccacgtagt ttttctgcac catatccagg
    12421 acttcattga aggttttaat ctccttgtaa atatttttgt cccgcgcgga aaccttgctg
    12481 tcggaatacg gcccgaaaac gacgaaaagc ccgaagagga ttacggccaa tattggccac
    12541 ttaccggaaa tatttttcat gctctgcctc actgcccgtc gatgatttta ccacacgcca
    12601 agagaatgac ttctatagct tcaagtatat accaatcttt tttttctttt ccatctattt
    12661 cggcaagccg taaaagaaaa taacggagaa tcttcagaaa ccgcgcaaaa gcgaatcggc
    12721 cggccgggct tctaatcgat gatccaggtg gtccggcccg cttcatcctt caaggcaatg
    12781 ctctgcaaag ccagctcccg gcgaatttca tctgcgcggc cccaatcttt agcgtcgcgg
    12841 gctttttgcc gctcggcaat cagcgcctct acccgatgaa tatccattcc gcgtttttcg
    12901 gcttcgatgt cgcggtcctt cgagaaaaag agatccgggt cggactgaaa cacacccagc
    12961 gcatcgccaa aatgacggaa ggcctcccgg gcggtctcca ggatggcctg tttgtcggct
    13021 ggaggcatct ttttttccac cgtcatgacc tggttgacga gccgggcggc gtcaaacaca
    13081 aaacccagcg cctgcgccgt gttaaaatcg tcgtccaggg ccaagtcgaa tttttccacg
    13141 gaggctctca atctggcagc gtatttcccg ccgtcggagt cggttttgac cccgcccaga
    13201 tgaacatccg ccgcctgcgc atccttcatc aaacgaaggg cggtataaca gcggatcagc
    13261 gcggtccgtg cttcccgtaa agagacatcc gaataatcca ccggacttcg gtaatggctc
    13321 tgcagcatga acagacgcag aacttccgga tggaattgcc ggacgatctc ccggatcggg
    13381 aagatgtttc ccagggattt ggacattttt tccgcgttga ttttcacgaa accgttgtgc
    13441 atccagtaac ggaccggagg ctttccggtc gccgcctggg attgggcgat ttcattttcg
    13501 tgatgcggaa aaatcaggtc ttcgccgccg ccgtgaatat caaagggctc gcccagatat
    13561 cggcggctca tcgccgaaca ttcgatatgc catcccggtc tgcccattcc ccacggactg
    13621 tcccaggaag gctcgccttc cttgcttttc ttccagagca cgaaatccag cgggtttttt
    13681 ttcttgtcgt tgacgtccac gcgggcgccg gccatcattt catccgggtt tcgtccggac
    13741 aatccgccgt aggcgggaaa tttggcaacc gcaaaaaaca cgtctccgtc caccgcgtag
    13801 gcaattcctt tttcctcgag agcccggatc agatcgatca tatcctccat gtgccccgtg
    13861 gctctcggcg tgaccgtcgg cgtcaggacg ttgagggagg ccatatcttc atcgtgcagc
    13921 gcaatatagc gctcggcgat ctcggcaatg ccgacgcctt cccgattcgc ccgatcaatg
    13981 attttatcgt caatgtcggt gaaatttttc acatacgtca cgtcgtagcc gcgcgctttc
    14041 agatggcgga agacgacatc aaacacaacc gccgcgcggg cgtggccgat gtggcagaca
    14101 tcatacgccg taatgccgca aacgtacatt ccgaccgcgc cttcccgaac cggttgaaag
    14161 atttcctttt ttcttgtctt cgtattatag attttctggg ccattttctt accttcgtca
    14221 gggatacagc gggggcgtcc cgagcccgct gtcttcgggc agtccgcaga ccagattcat
    14281 attctgcacg gcctggccgg cggcgccctt gatgagattg tcgatggcgg aaataataat
    14341 cacccggccc gtccgcaaat caacggtcaa accgatgtcg cagaaattgg atccgcaaac
    14401 ggaggaaatg ttgggataga cgcccgcggg acagatccgg acgaattttt caccggcgta
    14461 gaaggacgca tacagatccc gcagcttagc ctccgtcgtc ttttttttca gcgtcgcgta
    14521 catcgtgctc aaaatgcccc gcttgaccgg cagcagatgc ggcgtgaagg aaatgaagaa
    14581 tttttccccg gccagttgat ggagctcctg ctcgatttcc ggcgtgtgcc ggtgtttccc
    14641 gaccttatag gctttaaatc cgccgtccac ttcgcagaaa agcgatccga ccaccggatc
    14701 ccttcccgct ccgcttacgc cggaagccga atcggcgata atcgtggaga cgtccagaag
    14761 cttgctttga agcgcgggcg cgagccccag aataatgccg gtcggataac agccgggatt
    14821 ggcaatcaac ggcgatgttt tagccgcttc ccggtaaagc tccggaagcc cgtaaaccgc
    14881 ctgtttgatg agaccggcgc tggaatgttt ggcataccac tgttcatagg ttgcccggtc
    14941 ccgcaagcgg tagtcggcgg acagatcgat taccttcttc cccgcttcga tgaagacggg
    15001 ggcgatctcc atcgacacgc cgtgcggcag cgccagaaaa acgacatcac aaagctctgc
    15061 gatttcccgg ggcgccgcgt tggcatatgt gagaaaagtc aaaccggaaa gggaaggaaa
    15121 aacgtccgaa accttcattc cggcgtagcg ccgggaagtg acggccgtca gctccacctg
    15181 cggatgtgcg gatagaatcc ggatgagttc ctgccccgtg tagccgctgg ctccgaatat
    15241 cgctgctttc gttttcataa aaaactccaa aaaaaagggg agcctgaagg ccccccggaa
    15301 aattaacgtt tggagaattg gaatcttttg cgggcgcccg gctggccgta cttctttctc
    15361 tccttgatgc gggcgtctcg cgtcagaaaa cccgcttttt tcagaatggg gcgcaactcg
    15421 gcgtcgcatt ccagaagcgc tttggaaatc ccgtgcttca ccgctccggc ctgacccgcc
    15481 acgcctccgc cgctgacatt gacataaaag tcgaactggt cctttttccc cgtgatctcc
    15541 agaggctggc gaatgatcat tttcaggctg ggacgcgtaa aatattcttc atagttcctt
    15601 ttgttcacca taatgctgcc gctgcccgat ttcatataaa cgcgggcaat ggcgctcttt
    15661 cttttcccgg ttgcataaaa acgttgtact gtcatgacct ctcccaaaag taattaatcc
    15721 atgaaacagc ccggaccatc aaaccgtcag ggtttgcgga cactgggctt catgcggatg
    15781 tgcatttccg ctgtaaacct tcagtttttt tagcattttt ctgccaagat gattcttcgg
    15841 cagcatcccg gccacggcca ttcgaatcag attttccggt ttctcggcaa gcatcttccc
    15901 ggccgccgtc tctctgagcc cccccgggta accggagtag gaatagtaaa tcttatccgt
    15961 aagcttcttg ccggtcagcg cgatcttctc ggcattgacc actacaataa aatcgccggt
    16021 atcgacatgc ggcgtataaa tcgctttgtg cttgccccga aggcgcaggg cgatttcact
    16081 ggccagcctt cccaatatct tgccggaagc gtcaaccacg aaccaatcct gcttgacctg
    16141 tcctgctttt gctgaaaatg ttttagtcat aaaccgtcac ctggttaaaa atttaaaggt
    16201 ggaagctatg caattccacg ctctttgtca aggaaaatgt ttggaaaatg tttgaattcc
    16261 ttatgccgta gtctgaaaca cggcactgcc cccttcccaa cccccaaggg aaatggccgc
    16321 gcctgaccaa ccggtcgtcg cgtttaaaac ctgcccaatc ccgccagaga gaatttcagc
    16381 atgattcttt cgtcatcgta ggtcttggtg tagtccactc ccacgcccca gcactgcgcc
    16441 caataggcca gaccgaccgt attttccacc gtccggtcat taaactgatc ccgcctcaaa
    16501 ataagattcc cgctgaaccc ccgggtgagg ttgagtttta gggccatatt aatttcctca
    16561 agagaatcca gcgtatagcg ataccctacc gtcagattgt ctccgcgcca atcattgagc
    16621 cccagatcgt aattcatctc cttccaaccg acataaggac tgtatttatt gcgcgcggca
    16681 aaggaaacat aaggatgcgg cttcaggtca aattcgatcc ccagatccga aaccggccgc
    16741 cgctccgcag tggaaccggc catgtctttt ttggcttcgt tgatgtcgta aacctgaaac
    16801 agcttcaaac gcaacaattc aatataactg cgcgacccgg atttgtcctg cgtccttgcg
    16861 gttaccgtgt tggtcagcgc ccaggccacc gcattttgtt cttttaaaac actggaatag
    16921 ccgatattgg aagaaaggga gttggccatg aaggagttca cccagggaat gtaatcggga
    16981 agattgtcct ggttcacgct gggaatgtac gaatagaaaa tttcaggctt gatttcatgt
    17041 ttgattttgt cccagttttg aattttgacc gcaaacacgc gtgaaatccg gctgctgagg
    17101 ctcaggccgg cattatacac gttgcggttg ccgcttttgt cgtcggaatt ggcctgcgca
    17161 tcgtcgcggt tccagtaaac ctcccgcacg gtgatttgag gggtaatctt cgcgtaacgg
    17221 gaaatattaa aaggcaggga aatcgtcggc gcaatatcca cataatgccc tttctgtccc
    17281 tcgcccctgt aaaaataatc atagttgccc gcgaattcgt aatacagggg ggttgaaaaa
    17341 aaccgctgct tgatgcccgt caatacgatt tccggatact gctgcagggt ccgctcgtta
    17401 ttcacggctg aaaagtcgtc cgtgaaactg acgcgggcca tcaggctgaa gttattccat
    17461 cctttatata ccctggcgga cgattccagg gacggcagag actcgttgcc ctgaaaaggg
    17521 actttccgaa acgggtccag ctccgtcgtg gagtaattgg acagataata gttgtgggcg
    17581 ctaaaatccc tgaaatacca ggaatcggaa acccgcctca agtccgtccg cacataaaac
    17641 tgcgaatcaa aattcgtctg atgattcaaa taataagacc agcgtctgtg cgtctcctgc
    17701 cagtcgcggc tttggctgcc gaccgcggcg gtttccgtga cgtgcctgtt gtcctccata
    17761 aaatcgccgt aaagggtccc aaaagacttg ctgccggcga aataccggaa ctcaacaccc
    17821 tctttgaatc ctcttttttc catgtaccgc gaaaagaacg tcgcatccat ctggggggta
    17881 atcgcccaga aaaaggggag ctcgatgtcg attccgtctt tatccttcga atggctgagg
    17941 taggggaaga gaaatcccga ctgtcgcttg gtctttgcgg gaaaagcgac gagcggcgta
    18001 tagaaaatcg gcaaatgcct cgctctcaac cgggcgtctt tcatcaatcc atacccttcc
    18061 agcgtcacgt tcatcgaacg tccggcaatt tcccagtccg gccggtctcc gtcacaggtc
    18121 gtagccaccg gctcttcgat aaaataagtg ttctcgcccg ttttctcgat tctgctgcct
    18181 cggatgtaaa aatgattgcg cgcgtaaaag gctttggagt tgtaggccgt gccggtttta
    18241 tcttctacgt tgaccacgat tttatcgccc tgcaacgaat cctgggccat ccggagcagg
    18301 gcattgcccc gggcggttgc cagattgttt ttgcggttat aggaggcgct ggcggcggtc
    18361 aggacgccgt cgccgtaagt gatcactaca ttgccctcgg cttcgtaaag gtccctttcc
    18421 tgatcatagg acagcgtatc cgcctcgatt ttcaccggcg cgtcgtcggg cgctttcacg
    18481 tcctgggccc aaacgccgct ccgggcaaac cccaggacga aaatcactcc cagaatcatg
    18541 accgtatgtc gtttcagcaa aaccggtaaa atcttcatca tcccgtctta tgacatgcaa
    18601 gcaacaaaaa caccctcttc acttctcact catcgctttt cactttttct cagtgcgcct
    18661 ggctttacca caagaattca tttgatgaaa cgactgtttt acctcacccg caagatacag
    18721 agagcccaga gcgcagatca tatccccttc ttccgccatc gccagcgcca aagaaaccgc
    18781 ttcatcaacc gtccccgcca caaccgcttg aaagcccatt tttttcatca aaccggcgag
    18841 ctgctccggg ggcacggctc gcccggtcgg aagcggcggc aaaatcgcga cggacgccag
    18901 aggcgcgatc ttttgcagca tgttccggta atccttatcc gacaagacgg caaaaatcag
    18961 aatcaaacgc cggaaggaaa aatcttcctc cagcgcacgg cgtaaaaccc ggacgccgga
    19021 aggattatgc gcgccgtcca gcaggaaaac cggccgggtg tgaaggattt ccatgcgggc
    19081 ctcccagcgt gttttcctca aaccggcacg gattgccgca tcccttattt tcaaaccgct
    19141 ttcttctgca acttccaggg cggccagcgc cagcgccgca ttggccagtt gatgcccgcc
    19201 ggaaagcgca atgcgaagag agctgatctt ccggttgatc ccggaatagt caaccgtccc
    19261 gtctcttcgc cggatcgctt caaagtcctt ccccagacgg taaaaccggg cttttttccg
    19321 gcggcagacg gaagccagat gatccgccac cctcttctgc gtggcgccgg taatgcagac
    19381 gcctccctgt ttgatgatcc cggccttttc tccggccacc gccgccaggg tacggcccag
    19441 ataagccgtg tgctcgagtc cgatattggt gatgaccgaa gccagcggac ggcagacatt
    19501 ggtcgcatcg agcctgccgc caagcccgac ttccatcacc gcaatatccg cccgcgcccg
    19561 ttgaaaataa ataaacgccg ccgcggtcag cgcttcaaag taagagacgg gctcaggggc
    19621 tttgaccgaa atttccgata gaatacggcc cagatcgcgc ccgggaattt ttttgccgtc
    19681 cagcacaatc cgctcccgga catccaccag atgaggagag gtataaagtc cggtcctgaa
    19741 gcccgcctcc ctgagcatgg aagcggtcat cgcggccgtg gatcctttgc cgttggtgcc
    19801 cccgatcagc agggtgaaat aatcgttctg gggagcgccg aaccgctcca gcagcccggt
    19861 gatcgcctcc agaccgaaac gcatgacgtc gatattcagg ccggaaagat atgcgccggg
    19921 attcaaggaa ttcatggaat tgccggtaaa ttaaagatga atgacctgac cgtctttgat
    19981 gacgtgaatt tttctttccg gaatcgaagc gacttcgcgg tggatgattc ccgcatacct
    20041 cggcttcata tgataaaggt aaatatcggg tatggcgccc ttcaatttct tcagctccgc
    20101 cgcaagcgtc agcggcgtga gatggcccgt tttgtccgca aggctttgca ttccggcagg
    20161 cagagacgtt tccacaaaaa tcgcccggag gccttttgcg gccccggcaa ctttccacac
    20221 gtcttcggta ggccccgtat cgccgaggaa aagggcctcc ttcccctgcg actccatcaa
    20281 aaaccccatg gtttccacgg tatgatgaac aggaatggcc cgcacccgcc aatcgcccaa
    20341 tgttttttgc cggccgggag agagaatctc aaatttgacc aggggatttt tagcgctggg
    20401 gattctggaa aaatcaggcc agataacgtt gttgaaaaga tggcggtgga tgctttcaag
    20461 aatcccccgg ctgctgcaaa ttctcagggg ggtctttcgc ttcgcgtaac agagattgtc
    20521 cgcgagaaac atgatgtcgc gcacatggtc caggtgggcg tgcgtgacca gaatgtaatc
    20581 aatgagaagc tgctggcgaa gcgtcagcac ttgcgtaatg gcgcctgcat cgatcaggat
    20641 gtttttcccc agcagaaaac tggtcgtatt gtgattcggc aactgcgaac cgtggcaacc
    20701 caaaacccgt atcttcatgt caagctcccg ccgcctgaaa tcgtaaacgg cattcgtcaa
    20761 ggacgctatc ataccttcgg aaggcaggca atgtttttgt aagatcataa cgtcaaaagg
    20821 tttttcgata aattgtcccc catagaacat acaaatcaaa acgagtcaaa atcatgtctt
    20881 tcttccatga aaatcctgtt gaacaagaaa agggagagaa gcatccctct ctccctttct
    20941 ttttaaaaac gcggcgctga tcctgaaaat ccccgcctat tcattcatcg cgtattcacc
    21001 gtttaaggca taaacatatt ccttccagac ccgttcatac tggacgtcat ctccctgggc
    21061 cttgatcagc atgatttcct tgcagtaggc tcgctgttcg tctttcgtgt tgtgcatgcc
    21121 gtagatttgc agggcgaacc gggcaaaatc gcgcaccatg aaatcaaaaa tctgattcac
    21181 gatatcggga tcgacctgat cgaatttggc ctgctccaga atcaattggc cgtaaaccac
    21241 gatggagaac atctctccca gcggcagaga aaacagcgga tccatatcct gagccttgtc
    21301 cggaccggct ttctccagca attccctgaa cagctcaatc tgtttgatga agatggccac
    21361 attgggaagc tgctgattcg cttcgaaaac cggtttgtag tcatggaact gaacggaccc
    21421 cagacctttg gtcggtccct ggttgaacag gaacatgtca tccttgaggg agaaatcggg
    21481 cgcaaccggc ggatatgcgg cggggctaaa gaaataattt ttcatgaatt tacgaatcaa
    21541 ctgcacgttg acgtgaaccg tcccctccag cttggagggc ccccggatgt cgctggcgcc
    21601 gatggaaaaa taggtgtcct tttcaaaccc tttggcggca atgacttccc acagaagatt
    21661 aatcacgtcc tcggcctgaa gcgtcacctt catcttgctg gtgggattgt acagcaggta
    21721 gcgacgatcc tgcgcgttgg cgttgcggaa atagtcggtg gcccggcgct ggtagagctt
    21781 catgcccaca atccgcagcc aggcatccat gaaattcttc ttcacatgcg gcatggcggt
    21841 cacgggaatg ccgtaaagaa cgcggtttgc ggcatgggtg atggattcat aaaagcagtg
    21901 ttcggcgacg ccgagggaag ccgggccgat gttgaacttg ccgatattca cggtgttgag
    21961 cgcggaatcc caggcgtgcg gcccgcgcga cagaatatcg tcttcggtaa tgggataatc
    22021 atgaagcgcg aagttggaaa catattcctg atgggaaatg acgttcttct tcagctcata
    22081 ggccttgtgg cggtaattgg tgacaaaaaa agtataatcg tccgtgccgt ccttcacctt
    22141 gcccagcgtg gaaaccattt ccgcctcgtt gccgttgccg atgtagtatt tttccccgtt
    22201 ggccacccag gttccgtccg gtttgggcgt caggctggtt tccgtggaat aaatatccgc
    22261 gccgtgcgtc ttttcggaca ggccgaaggc gaagatcgcc ccttttttga gcaggtcggc
    22321 cgctttttgc ttggcttttt cattggggct catccagatg gggccaagtc ccaggatact
    22381 gacctggaaa cagtaccagt agcccagacc ataaaaggcc agcagttcac tgtattcgct
    22441 gttgcgggcc gtgtcccagc ggcaatccgg atcgcctccg ccgtattgac tgggagtgag
    22501 aagtttggca aagatctgtt ccttgccgat aaaatcaaca aactcgcgat accagacttt
    22561 cttgttgtaa tcctccgtga tgcgcgtttt acccatcttc tcaaagaagg cgatggtttt
    22621 gtccatgatc gcctttgaac cttcatcgga catcagggac tgatacttct tcggctgcag
    22681 cagataattc atacgggact cctttcatcc tgttaaaatg agttttcaaa tgtgcaatta
    22741 ctctatttca aagaaagaag ctacttcaaa attattcctt cacggctcct tcggaagcag
    22801 gctctgccag gtgtgccgct ccagaaacga accgggaata ttgcgctcca tctcttcgaa
    22861 cagggcaaaa ggcgcggcaa aggtcatgaa gttgccgtca cgcagctgct tgcgcacatt
    22921 gacgcgggcc gaaagatcgg tcagccccac caccgcgcgg ggtctgtccg acctggcctc
    22981 ccggtaaggg taaatgccga tattctgaca gccggcggca tacggcatga tgacattttc
    23041 attgtcgccg cgcgcgtaat tggccagcac cgtcagcgcg gaaagctggt cgggattaac
    23101 gaaaagcagg atcgtctgcg gtttttccgt tgccggatcg accgaagaca agggctggaa
    23161 aaccacgtag gcagccggca catccgtcat cggcagggcc tcaacaaatt tttccgccag
    23221 ctccggattt ttgatatagc gctcgccctg cagaaaattt tcataactct ctttggtcat
    23281 gaacggtttg atcttttcgg ccagttccgg gccgccggga cgcaaggcat tgcccgacga
    23341 cagaaaacgg cagaatccct ccataccgcc gggaaaattc agatactgat taccgaagcc
    23401 cagccccacc ccgccgccga cacagccgaa cgtttttcga tccgccacgg cgggtctgcc
    23461 cttggccgcg gaaacggcga gccacatcac gcaaccccac ttgccctcgg aaaactgcat
    23521 cgcgccttcc ggcttttcat ccgaccaaaa aatcgccacc ggttgatatt gaagttgaat
    23581 tgctttgggt atttggcttt ccatgttgaa tcctcctgaa catttggccg gattatacat
    23641 ttcgctttgg aaaaaataaa gattcttttt cgcccggtcc ggcgatcccg cgccctgccg
    23701 gcgtcgtttt taaagccccc ggactggagg acggcacaaa actttttcaa aaaatatgcc
    23761 ttgaagcctt gacacgcaaa acagccttgg cctatactcc aaacgctcaa aaatatttct
    23821 cattgatttt ccatagaagc cgagacaacc aggaggagaa cttgaagaat ctgacaggca
    23881 gctcatgatc cggtcaccgg cagaggaagc ggcgcagggc ggcggattcc atagaaggcg
    23941 gcagaaggag tcatcccgca gccatcattc gaaagagccc gcaaaaaagg ctttaaacca
    24001 gcaaggagcc gaacatgaat cgagcgtttg cgaacatcag ggaaatggtg gaaacacagg
    24061 gtcgccgctg tcaggacagg atcgccatcc gcttttacga ccgggatatc acgtaccgtg
    24121 acctggatga cgagagcaac cgcatagcca acggcctttt gaagctgggg gtcggaaagg
    24181 gcgaccgggt ctgtctgctc atggataaca gcccggaatt ttactacgta tattttggaa
    24241 tcatcaaaat cggcggcatc gccggtccgg tcaactgctg gtggcaggcc gcggaaatcc
    24301 ggtacctgct gcaggattcc ggggccgtgg ccctgttttg cgatcacaac tatcggggac
    24361 tcgtcgacca aatcatcggt gaaacaccgg acatgcagca cgtcattgaa cgggaagcgg
    24421 ctgacgatcg ttttctgccg ttcgaaaaaa tgctgacgga gccggtgtcc cttgcggatg
    24481 cggcgatcag ccccgacgat gtgtccacga tcgtctatac ctcgggaacc acgggaaacc
    24541 cgaagggggt tttgcttacc cacagaaaca tcctgaccaa ttcctggcag gccagtctgc
    24601 tggccaacat cgatgagaat gatgtcattt tgtgcttcct gccgctcttc cacgtcaacg
    24661 gcctcgtgat taccggaaca tctcccttga gcaaaggctc ccagatcgtc ctgcgccgca
    24721 acttttccgc gtcggaattc tgggaatgcg tcgcgaaata ccgcgtgtcg atcttcagcg
    24781 gcgtgccgac ggtttatcag attctgctca acacgccggg gtttgaggga ttggacgtgt
    24841 cgtcgctgcg ctacggcgtg tgcggcgcgg cccccatgcc ggtggagacc atccgcaagt
    24901 ttgaggaaac gtttcacatg atcatcattg aaggctacgg gctgaccgaa agcacggccg
    24961 gggcaacggc caaccccatc aacggcgtcc ggaaaatcgg ctccatcgga attccgtttg
    25021 cgggaaccga aatccggatt ctggacgatg cggaccagga ggtcccccag ggagaggtgg
    25081 gagaaatttg catccgcggc gaccatgtca tgaaaggcta cttcaacaaa ccggaggaca
    25141 ccgcccagac cctgcgcggc gggtggctcc acacggggga catggcctat aaagacgagg
    25201 acggctacct gtttatcgtg gaccggaaaa aggaaatgat catccggggc ggtgaaaaca
    25261 tctaccccaa ggagctggaa ggcatcatct gccggcaccc cgacgtcgcg gaggtagcgg
    25321 tagtcggcat ccccgacgcg atctacgggg aagaggtcat ggcctgtctg gttctccatc
    25381 cggaccggac gctgacggag gaagcctttc aagactggtg ccgggccaac atggcgtcct
    25441 ataaggtgcc gaaatacgtt aatatccgcg cctccctgcc caggaacatt ctggggaaac
    25501 tcctcaagaa agaactgaaa gccgccctga agtcggaggg aaaaatctga ccgggaccgg
    25561 cgggggaccg cgcgaacaga aatgattaaa attttcagga ggcaaccatg aaaatatcat
    25621 ccattgagct ttatcatgtg caggttccct tgaagcagac gttttggccc acgtggatcc
    25681 ccggctatcc gcagacgcac aacgggttca cgctgatcaa gctggtcacg gacgacggca
    25741 tcgaaggata ttccgcgggt tccgccctgg gacttgagcg gcaaggtttg ggcgatctca
    25801 tcggcgggta tctgctgggc gcggacccga ccgacatcga atcggtgcag gccctgctga
    25861 aacaggccgg catcctggga tggagaaatt tctggatcga gccggcgtgc tgggacatca
    25921 tcggcaagag caagggaaag cccgtctatg aacttctggg cggcgagaaa cggcccatcg
    25981 aggtgtactg ttccacgggg gaaatgcacg aaccggaaaa gagagtggaa gagctcctgg
    26041 ccatgaagga gcgcgggttc aaaacggcaa aactgcgggt aaaaaacaag gatctgaagg
    26101 acgacatccg acatatcgag gtcgtccgga aaggcatcgg agaccggctg gttttaggcg
    26161 tggacgccaa ccagggatgg ctggtgacga ttgtggacaa aattcccgcg tgggacctga
    26221 agcgcgcgac ggcctttgcg gaagtctgcg ccgcgaacgg catctcctgg ctggaagagc
    26281 ctctggattc gcgggactac gacggcaatg ccgccctgaa aaaagtctcc aaagtcagaa
    26341 tctccggcgc cgaactcaac tacggatggg atgagatcaa gatcatgttc gaaaaggact
    26401 gcttccacat ttaccagcct gacgcaacct tcgccggagg aattgcccag gtgaagaagg
    26461 tgattgacgc cagccatgcc caccaacggg aatactcccc gcatacctgg accaacggaa
    26521 tcgggtttta cgtaaactgg aacctcgtcc tggccgataa aggcaatgcc cttcccctgg
    26581 aataccccct ggaggagccc tcctgggcgc cggaacaaag agaagggatc atcgacccga
    26641 tccttcccga ccaaaacgcg atgcttgcgc cgtttacaaa gccgggcctt ggatttgaga
    26701 tcaaccagag cctgctgaac aaatacggaa agcggttttt caagctgacg gaactgcgac
    26761 agaaaatcaa ggtgattcgc cagaaaggcc tgaagacagc gctggcactg aagaaaagaa
    26821 aagaatccca gtagcctgat gagggaagtc ctccgcattt cgacggccag gtccgggata
    26881 ttttcggaaa ataacagccc cagccttctc tatcccaact ttccgggggt caccgccggg
    26941 tttgcgccga gctcgatgat cgcataccgt cccttcttga agcttccggt gttgacgacc
    27001 aacgtgtttt gaaaacgttc aatgccatgc gcctcgtgaa tgtggccgca gagacagagg
    27061 ccgaccggat gggcttcgag aaatgctttc acactgtgac tccccccgcg caacccgatg
    27121 aaggaccggt cccggacgga acgaggagga gcatgggaaa tcaaaaccag ccgggaagcc
    27181 tgccggacct gctcatatcc tgtttttaaa atccgggcga tttcttcttc cgaatatacc
    27241 gtggcggtag gaatcggcgt cggcgacgac ccgccgacgc cgaagaatcc cgtcccgtcc
    27301 agaatccggc ccctgccgtg cagagaaaaa cctttttctt caagaacgtc tttgacttcc
    27361 tcccgatccc agttgccatg cacggcgagg atccgttttg aatgttgctc cagacaccca
    27421 agaacatcct ttgcctcggt ccgcgttttg gtgtgcgtga tgtcgccgga aatgaccacc
    27481 gcgtcagcct cgcggataag atgggcagct ttttcaatgg accgggtagc cccgtgaata
    27541 tcgctgacgg caaaaatttt catatccatt catccgggga gttcaatgat ttttccatgc
    27601 tcttccaatc aatggaaatc tcgaccaacg cctgatttcc gtcatcctca cgttatgcct
    27661 ttacgggtcc gttcaacgga aagcacagcg atacggttgt gcctcgtccc ggccggcttt
    27721 cgatttccat cctgccgccg tgcgcgagca tcagatgttt gacgatcgac aatcccagtc
    27781 ccgtgccgcc cagctcacgc gagcgcgcct tgtccacgcg gtaaaagcgc tcgcccaggc
    27841 gctgtacttc ctccacggga attccaatgc cggtatcgct gatcttcaaa accacctgat
    27901 cgcctctttc ttcggcttcc atcgtgatcc gccccccctc cggcgtaaac tttacggcgt
    27961 tgtccagcac gttgaccagg atctggctga gacggtcccg atccgccttg atgggcctga
    28021 gattctgcgg cagccggttt tgcatggcga tggttttggc cgcggctttg gcatccagaa
    28081 gggagatgac gctgtccatg gcttccggaa gaaaaacagc ttcgaagcga aaaaccacct
    28141 ccccgagttc aatcctcgac aggatcatca ggtcttccac caggcgatgg agccgccggg
    28201 cctgtttgag catgatatcg gtgaagcgct tcgcatcccc gggattctcc agggcgccgg
    28261 cctgaagcgt ttccagatag ccgatgacgg ccgtaagcgg cgtgcggatt tcatgcgtga
    28321 catttgccac aaaatccgtc cggacacgct ccagtttctt cagccgggtc acgtcatgaa
    28381 agacgatcat gactttttct tccccgggaa gcccctgaac ttcggacagg ctgacgttca
    28441 tgatcacggt ttcggcggct cccagagcaa tttcgcgcaa aatactttgc cgggtctgct
    28501 taaaactctg aaacgccttc tgaagttcgg cattgcgaaa agcttccaga agcgtcttgc
    28561 ccagaaagtc ttcgtattgg ccggaaagaa tgccggccag caacgggctt aaaaattcga
    28621 tccgttcatc tctgttcaga atcacgacac cttcgttgat gctggtcaga gcggtcatca
    28681 gtttgctttt ttcctcgttg gccagccgga tcttgtccct gagctcctcc accagatagt
    28741 tgatgttatc cgccagcgtt cgggtttcat cggacgtcct gagcatgatg gagccgacgg
    28801 gttgccccct gcgcagccgc tgcgtaaatt cctccatcat ccggatgggg gcggccagcc
    28861 gatgggaaaa aaacagggcg accgccagag acaccagcgc gccgatcagc atcgccagga
    28921 aaatggactg ataaacttga tcaaccgcgc tctggacatc atgaagcggc cttgccagac
    28981 ggacatagcc cctgctgttt ttcccgtcct ggatggccac ggccacataa agcatgttca
    29041 cgcccagcga actgctgtaa cggacagcct ttccctttcc tctcagcctc gcctcctgaa
    29101 tctccggacg aaacagatgg ttctccagtc cggcgatcgc cttttccgaa tcggcaaaaa
    29161 cgacgccccg ctcattcacg agcgtcacgc gggacttgga tattttactg atctgctgaa
    29221 gatgcgcgga catggcctgt ggcgcgttca gatccaccag ctcggcataa tgcatcaatt
    29281 cctgttcgat tccggctgtc atgattccgg atatccggtc tttgacgagc agatgcagga
    29341 taaagaaaga cacgctgatg atcaccagat aggaaaataa tattttgagg aataaattgc
    29401 gtttcacacg gcctcctcag gtcgtcatgc cgcaggattg atggcgcacg caatttcctg
    29461 tcaccatgta ggttaccatg tcggcgattc ccttggcgtg atcggcgatc cgctccatgt
    29521 tcttggaaat ctgcataacc agaagagccc tgtggatatt tttggaatcc tccatcataa
    29581 acgtcaggag ctcgcggaaa atctgctcgt tgagatcgtc aatcacctgt tccagcctgc
    29641 ggacttgctc cgccttttcc agatcgctgt tgataaagct gtccaatgaa tccctgatca
    29701 tgtttccgac cagttcggac atgcgcggca aatcaatata gggcttgagc tgcggctctt
    29761 cgttgagccg catgaccttt tcggcgatgt tcacggccat atccccgatg cgctccaaat
    29821 ggccggtaat cttgatggcg gttgtgataa agcgcagatc gatcgccgcc ggctggcgaa
    29881 gcgccagaat cttgatgcag cgctcttcca ggtcggtgtc caggcggtcg atttcgtcat
    29941 cctcccggac aacctgatga gcaagttccg tatcgcggcc ggacagggac ttcatcgcct
    30001 tgccgatcgc ggcctcggtc agcacaccca gatagatcag gccgtccttg atcgtctgca
    30061 attccttttc ataatgcaag ctggtgtgtc gtttttcttc catgattcat cctcccgaaa
    30121 tgatcaaccg aagcggcctg taatataatc ctccgtctgt ttgacatccg gcttggtaaa
    30181 gattttctcc gttttgttgt attcaatcaa attccccaga taaaagaaag ccgtttcatc
    30241 cgaaacgcgg gcggcctgct gcatgttgtg cgtcacaata atgatcgtgt aattttcctt
    30301 cagctcttcg atcaactctt caatcttggc ggtggaaatg ggatccagcg ccgaggtcgg
    30361 ttcgtccatc aataagatgt ccggcttcat cgccagcgcg cgcgcgatgc acagccgctg
    30421 ctgctggccg ccggagagat tcatggccga tttgttgagg atatccttca cctcatccca
    30481 caaaacggcc tgtttcagac tttgttccac cagggcgtcc atatcggcga cggaagtccc
    30541 ggtgagcttg ggtccatagg aaatattgtt gtggatggtt ttaggaaacg gattgggttt
    30601 ctgaaaaacc atgccgatct tgcggcggat ctccaccgga tcgacgtccg gcgcgtaaat
    30661 gttccggcct tcaaaaagga tttccccttc caccttcgtt gcgtcaatca attcattcat
    30721 ccgatttaaa agccgcaaca gcgttgattt gccgcatccg gacggtccga tcagcgccgt
    30781 caccttattt tttttaaacg tcatcgagat gtccgtcagg gcacgcacgg cgccgtaata
    30841 aaagttcacc ctgttgacgg aaataatatc gcctgcttcc atggctacca ccttttattc
    30901 cttctcatag acccgcggat caagatcgcg atcaggtcaa tgcccagaac caccgcaacc
    30961 agcaccagca cggtgccgaa ctggatgggc cttgtggcct caatattcgt tcccgccgtg
    31021 gccagaacat aaatatggta cggcagggcc atcacttcat caaaaaccga tcccggcagt
    31081 ttggcggtga aatacgcggc ggccgtgaac atgatcggcg ccgtttctcc cgcggcccgc
    31141 ccgatgccca gaatggagcc ggtcagaatg ccggggagcg cgttgggcag cacaatccgg
    31201 taaatcgtct gccacttgga cacccccagg gacagagagg cttcgcgaaa ggtctgcggc
    31261 accgctttga gcgcctcttc cgatgcgccg ataatggtcg gcaggatcaa aaatcccagc
    31321 gtcagagagc cggaaagaat actcgagccg aattttagaa aaataacgaa aaaccccagg
    31381 ccgaaaagac cgaaaaccac cgagggcacg ccggccaggc agttgattcc aaccttgatg
    31441 agcctgatca cccggccctg cctggcatat tcggtcagat aaaccgccga agcgatgccc
    31501 agcggaagtc caatggcaat cgcgccgatc gtcagataaa tcgtgcccaa aatagcgggc
    31561 atgatgccgc ctttggtcat ggaatccgtc ggcggtttgg tgatgaagtc ccaactgatc
    31621 acggagatgc cgtttgccag aatatacaaa agaatgccgc ccaatgctag cgtgatgacg
    31681 agcgccgagg cgcgcaccag aacaaaaaat acgttctgtt tgaagtaacg ccagcgcatg
    31741 gaatggtttt tcagattctt catgcgcctc ccttacagcg tcgcggaacc ggtctggcgg
    31801 aacttgtggg acacataatc ggcaatcagg ttaaacgcca gcgtaagaaa gaacagcacc
    31861 atgccgatgg cgaaaagcgc ctggtagtgc gcgctgccgt acggcgtctc ccccatctcg
    31921 gcggcgatgg aggccggcat cggacgaacc gggtcaaaca gactctccgg aatcgccgcc
    31981 gcgccgcccg ccaccatcaa cacaaccatg gtctcgccga cggcccgggc catgcccagc
    32041 atgaccgccg ttgaaatccc ggagagtgcc gcaggtacga tcacctgcgc aatcgtttca
    32101 aatttcgtgg cccccagcgc gtaggaggcc tccttgaatt cgcggggaac ggcgtagagc
    32161 gcgtcttcgg aaatgctgga aatcgtggga attgccatga tcgccagaat aacggaggcg
    32221 ttgatgatgt tgagccccgt gggcagatcg aacgtctcct gcagccaggg cgccacgatc
    32281 accatcccga aaaatcccag aacgacggac ggcaatcccg ccagcagttc aatgacggat
    32341 ttgagaattt ccttgatgcg ctgcggggcg atttcagaaa tgtaaatcgc cgccatcacg
    32401 cccagcggca cggcaatcag cgtggcaatc agcgtcacaa taagcgaacc aaccaccagc
    32461 ggaaaaattc cgaaggaggg cgggtcgtag gtgggatacc attcttttcc gaaaagaaaa
    32521 tcttttacgg atacggatgt aaacagcggc aggccttccc gaaaaagaga aaatacgatc
    32581 aatcccagca ccagaacgga aaccagagcg aaaagcagga aaatgtattg aataataatt
    32641 tcttttgtct gtctggtcat aaaagctctc tgaaaaataa aaccgcagca gaagcccggg
    32701 gcattcaggc gatggacacg ctaatggaat tctttttcta tccatctttt tatttcgtcg
    32761 cgaacccgcc ggtattcctg aatcaaatct tcagcgctgc cctgaaatcg cgaaggatca
    32821 gagaaaccct tgtgcatgcg aaaacgtccg cccggaaaat acgggcaggc ctctttggcc
    32881 tgatcgcaaa gtgtaatcac atagtccagg ggaacctctt gaaaaacaca gaaccccttt
    32941 gaacgatgat gactgacatc aatattcgtt tcactcataa ccgttttaac cagaggatca
    33001 atttgcgagg gatcggaacc ggcgctatag gccgcgtacc gatccccgta aatattgttc
    33061 agaagcgcct ccgccatctg agaacggacc gcgttatgcg tgcagataaa taaaacggtt
    33121 tgtttgggaa aattcattat ccaatatccc cggcttttta tttcttgccg cgcccggata
    33181 ctttttttgc atccattaaa ggaacaaatc cctctctggc gacaatcttc tgtccttcag
    33241 cggatttgac aaaggcgata tatttcgcaa tcgatccgtg gggttctcca tcggtgtaca
    33301 tgtaaagttc tctggagaag ggatatgctt tggacatggc cgtctgaaga gaagccgtga
    33361 tcccattcac ctgaaggggt ttgacgcttt tattgacata accgatgccc aggtaggcaa
    33421 ttgcatactt gtttttggac accgcggtga ccagcgcgcc gttggaggcc agcatctggg
    33481 ctttgggggt cactttggcc tttttcatca caaagtggtc ccaggactca aaggtgccgg
    33541 aagaagaatc gcgcgagatc accacaatct tcaaatcatc gcctccgact tccttccagt
    33601 tggtgatttt gccctgataa atctggctga gctggtcaat gctcagattc ttgactttat
    33661 tcttgggatg aaccacagga ataatcgcat cgtatgccac cacgtgggcc accggagtga
    33721 cgcccttgga tttggcaagt tcgatttcct tgtccttgat ttcacgcgat gacgtggcaa
    33781 tgtcggttgt cttgtcgatc aaggccttga tgccctcgcc ggatccgccg ccggaaaggg
    33841 acatctgcac gccgggattg gctttcatga aggcttccag cgtcccctgc gccaccggca
    33901 aaacggtggt cgatcctttg atgacgatgg aatcggcggc aaaagacacg ccggcagcca
    33961 tcaaaaacgc gatgctgaga agcgctgctt tttggaaaat actgaacata ggtacttcct
    34021 cctgttttta gattttccga ccttatacgg gatgaatgtt acagtttgat gacgcgttaa
    34081 tgaatttgtc aaaacaatgc caaagcaggg agcccgcgtg agagaagatg gttttctacc
    34141 gtatgtctgc gaatttatat ccgaatccgc ggactgtcag aatatattgc ggtttttcca
    34201 tattttcttc gattacgctg cgcagacggc ggatatggac atccacggcg cgatcggtaa
    34261 taaaggtgtc gtcgccccag acctgcgtaa gaatctgatc ccgtgaatac acacggccgg
    34321 ggttacgcga cagaaagaaa agcagtttca gttcagtggg actgagggtc accggacggc
    34381 cttccacacg cacccggcag gatccgtaat cgatggacag cccctgaaat tcaaagcgct
    34441 cctttgcgga aggtttcttt ccctcctggg cgcgcctcag aacggttctg acgcgggcaa
    34501 ccagttcctt gacgctgaag ggcttggtga cgtaatcatc cgccccgatt tccaatccga
    34561 tgattttatc gacctcgtcg gccttggccg tcaacatgat gatcggcagg gatgaagttt
    34621 ccggattggc gcgaatcctt ttgcagatat ccaggccgcc gacgcccggc agcatcagat
    34681 ccagaatcat cagatcgggg gtaaacgacc ggacggcggc aagagccccg tttccgtcca
    34741 acacgatcct ggcgcggaag ccttcccgct ccaggttgta ggaaatcagg tccgcaatat
    34801 ctttttcgtc atcgacgatc aggattttcg tcattgcgct ttttatcatt gtttgccgtt
    34861 caaaaacaac cccctaaaaa gtcaggagca gttccttttc ttttcagggg gttatgcgct
    34921 tttccttccg gattgccgct ttagaacgtc agcgtcagct tgttgatgac cagaaaatcg
    34981 ttggtcagtt cgttggcgtc ggaagttcct ttatagtact tgccggtgaa aagataaccg
    35041 ccgcccagca tataggaaag gttattggtg attttgtagg ttgcggtcaa atccacttcc
    35101 catccgtaat cgttgtgaag gtacacagtg gcaggattgc cggccgtgcc ccagggcttt
    35161 tgatcggcaa acgcatacgt aacggacgcc agcacatcca gcttttcgac cggtttaacg
    35221 ccgccgcgca tttggaacag ccagccgttg gccatcgccg tatcctgagc ggccccgttg
    35281 tatcctgtca gggcgcctgc ccagttggtc cggtcatagt tccacatgat cagacaggga
    35341 ttccagtcgc gcccgccgtt gataatgccg ccttctattt tgtcgttcgt cccgggatca
    35401 ttgccggaca catacgcgat actgccgccc gcatagaata tgccgaaatt ggccgtcgca
    35461 tcgatccagc cgctgatgcc ggccaaatcc accttatcga tgcctggcgc cccggccgga
    35521 ttggtgccgt cttcatactt gtcgcttccc gtcaggtaat tgagttccgc ctggagggag
    35581 accgggccga ttttcgcgaa agcataagga gtcaccagga aataggttct cttgtaagcc
    35641 ggcgccgcgg gacggttcgc ggcttgatgg taatagttga tgttcacgcc ggctttgccg
    35701 gtcttccagt tgtaaacgcc ttcgatgccg tatttatcgt tatcggcatc cgcccggttt
    35761 actgcgttaa tggccgtgcg gctgttttcc gcttctttgg tataggccag attgagcgtg
    35821 atgggtccga agacatttga atacttgatt cttgccttcg gactatagct gttgccgaaa
    35881 atggttcccg tcgaaccgtc gttcatgtaa ccgacgtcaa aaattccgat gggcgatgca
    35941 tagtggatgt aaacccagtc aacggcaatg ttttcgtttt ccgcgcgcgt tcccgcggaa
    36001 tcggctgcca gcgccgttcc agtggcgctt ctggtcgctc cccaggctcg ctccatcgcg
    36061 tccaggcgtg tgatcagggt caaccccgga gaaatggtga aatccgtacg aaccctcaac
    36121 cgctggtaat aaaaagcggt gctgggatcg ccgcccgttg tggtcttcgt taagtttgtc
    36181 ttatccagat acatccccgc cgcgtaatac tcaccgctga ccttgacgtc aacagccatc
    36241 gctgacgtac tgaaggcaac catcagcccc agtgacagca aaacaaacca aaatctcttc
    36301 atactctcct cctgaaagtt tttttcagag gtcggccttg gatcagcgca tcctctttca
    36361 tcaagcgtcg gcacgataca gagttatgat gacaggagga tgacaagtcc atgacaaaca
    36421 aaataatttt gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgaagatt
    36541 aatcgggaat cactttaaaa acttcatctc cgggccgtac gcggtccgag actttcaggc
    36601 cgatggaatc acccgccacg gctttctgca ccggcttgtt gtccacttcc atggactgaa
    36661 tcacgtcggt aaattccgtt gtgtggccgg aaaatttgat gctgtcgccg atcgacaatt
    36721 cgccttccga aagatggacc gccgccacgg aaggcttcgc aaaaaacttg agcacttcac
    36781 cgatcttttt ttctgccatc acgactcctc ctttgcgata gtgattcatc aataccttga
    36841 ttgatttcaa aaaacaacca caaaaaaagg cagggagcgc gccctgcctt tgataattga
    36901 ttttattggc tattcagcct ttttttcctt cttcttcggc tttgccttct ctttcctgtt
    36961 ctcgtccgga atatattcca gaatggaaac cggcgcgttg tcgccaaagc gatagccgac
    37021 tttgacgatc cgggtgtagc cgcccgcgcg attgcggaag cgttcggcca gttcgccgaa
    37081 aagcttggcg accatctctt tgtcccgaac caccgacagc gcctgacggc gcgcgtgcag
    37141 gtcgcctctt ttccccagcg taatcattcg atccgccagt ttgcgcactt ccttggctgt
    37201 cgcatcggtt gtccgaatac tttcatgttt gatgatcgat gtcaccatat tgcgaaacat
    37261 ggcttcccga tggctggacg ttcgtcccag ttttctgccc gccttaccgt gatacatgat
    37321 tacactatcc tttcctgacc gtctcccgat gattcatcaa acgccgctct gcccgcggtt
    37381 tgagttccgg tcgctttgct gttttaagcg ttttcctccg gcttcatgcc aaacgcaagg
    37441 ccgtattcgt tgagaatatc cttgatttca ctcaaggact tgcgtccgaa attcttggtc
    37501 ttgagcattt ccgcctcggt tttctgcacc agttccccga tggtgttgat gcccgcgttt
    37561 ttcaggcagt tggccgagcg aacggaaagc tccagatctt ccaccgaccg catgagaatt
    37621 tcattgacgt tgccttttcc atcctcgccc tgttccggag cggcatcttc ctcgacttcc
    37681 tcaaaattga tgaatacatc cagctgctgc ttcaggatct tggccgaata agcgatggcg
    37741 tcgcccgggt tcaggcttcc gtccgtccag acttccagca ccagtttgtc gtaatccgta
    37801 atctggccga cgcgggcgtg cgacaccacg tagttgactt tcttgatcgg cgaaaaaatg
    37861 gcgtcgatat tgatggtgcc ttccggctga tcgggctcca gctctttttt cgcggcgata
    37921 taccccttgc ccattttcac gaccatttcc gccttcagcg aaccgccgcc ggacagtgtg
    37981 gcgatataat gatcggggtt taaaacctca atcgttccgt cggtaatgat gtcgcctgcc
    38041 gtaatcacgc cggcgctggt cacgttgaat tttacgactt tcgtatccgc cgcgcccagt
    38101 ttgaaacgaa cgcccttcag attcaaaatc acgtccgtga catcttcctt gacgccgggg
    38161 atcgtggaaa actcgtgcaa aacaccatcg attttgacgg aggtaatggc cgctccctgg
    38221 atggacgaca acaaaaccct tctcaacgcg tttcccagtg ttattccaaa ccctctttcc
    38281 agaggttgaa tggtaaattc tccgtagaac cgtgtatgcg tggcttcatc gacatcgaca
    38341 cgtttgggac gaattaaact cgaccagttt ctctgcataa tcaaacccac ccttaagttg
    38401 gtttattcaa tctgttggtt gcttacttgg agtaaagttc cacgacaagc tgttcctgca
    38461 ccggcatcgt cagatcttcg cgaaccggca gcagtttgat ggtcgcttta aagttgtctt
    38521 tatccaatcc cagccaatgc ggcacaccgc gcctggcaac ggtttccatg gcttccagaa
    38581 tccggttgat cttccgggag ccttctttaa cggttacttc atcgccggct ttgagcaggt
    38641 acgacggaat attgacggcc ttcccgttca ccagaaaatg accgtggctg atcagctgcc
    38701 gcgcctcgat tcgtgaattg gcaaacccca ggcggaaaat catattgtcc agcctgcgct
    38761 ccagaaggac cagcaaattg gtgcccgtaa tgcccttttg gcgatccgcc ttttcaaagt
    38821 agccgtgaaa ctgcttttcc agaagtccgt acattctttt gagcttctgc ttttcgcgca
    38881 gctgggtgcc gtagtccgac tgctgtttct tgtgggactg gccgtgatct cccggcgcgt
    38941 agcctcttcg ttcaaaagaa catttctccg aataacaccg atcgcccttc aaaaaaagct
    39001 tcaagccttc ccgacggcac aaacgacatg aggaatccgt atatcttgcc aaaaaagcct
    39061 ccctttactc ttctcaagta ttcttgaaat atgaatatta taccctgcgc cgtttcggcg
    39121 gtctgcagcc gttgtgcgga acaggcgtaa cgtcgcgaat catcgtaatg gtcaatccgg
    39181 cgatctgcag agacctgagc gccgattccc gcccggcgcc cggacctttg atataaacct
    39241 ggacgcggcg catgccctga tccttggctt tgcgcaccgc gtcttccgcg gccatctgcg
    39301 cggcgaaagg cgtgctcttg cgcgatccct tgaatccctg gagtcccgcg gacgaccagg
    39361 aaatcacgtt gccggtcatg tccgtgatcg taacaatcgt attattgaaa gtggattgaa
    39421 tatgggcaac cccttccgta atgtttttct tttcttttct cttgcctgtt ttcctgactg
    39481 ttttcgccat tttccctccg gtcgaaaata ttttcagcta ttttttcttg cccgcaatgg
    39541 cccgtctcgg accttttctc gtccgtccgt tggtatgggt tctctgcccc cggaccggca
    39601 gccctttgcg gtggcgcagg ccgcggtaag cgccgatgtc catcaggcgc ttgatcgaca
    39661 tggaaatctc acggcgcaga tccccttcga ccttcccctc cttatcgatg atgcttcgaa
    39721 tggccgaaat ctcggagtcc gccagctggt cggtcttggt gtccggactg acgcccgatt
    39781 ctttcagaat ctgcttggcg cgggttttgc cgatgccgta aatgtacgtt aaggcaactt
    39841 ccattcgctt gtttttcggt aaatcaacac ctgaaattcg tgccacctat aacctcctga
    39901 tatggttgaa actagccctg gcgctgctta tgtttggggt tttcacaaat cacgcgcaaa
    39961 acacccttgc gtttgacaat cttgcacttc tcgcatattt ttttcactga cgatcgtact
    40021 ttcacgactc ctgctccttc tccccggcaa atgaggcttt cacttcgtcc tgtaggtgat
    40081 ccggcctcgg gtcagatcgt aaggagaaag ctccaccgtg actttgtccc cgggcaatat
    40141 tttgataaaa tgcatgcgca ttttgccgga tatgtgcgcc agaaccttat gtccgttttc
    40201 cagttccacc cgaaacatgg cattcggcag gggctccagc acccgacctt caacttctat
    40261 tgcttcctct ttggccatac attttcaacc gccggttaaa tgaaaaaaat caattcagcc
    40321 tgctcaagat ctccggtccg ttgtccgtaa tggccacgga atgctcgaaa tgagccgaca
    40381 aacttccatc ctccgtaatg accgtccatc cgtccggcag aattttcacc cgatacctgc
    40441 ccgcgttgac catcggctcg atggcgagaa tcattcccgt ctgaagctcg attccccgtc
    40501 ctttttttcc gaaattcgga atctgggggt cctcgtgcat attcctgccg atgccgtgtc
    40561 cgacaaaatc ccgaacgacg gaatacccgg cggattccgc cgtttcctga acggccgccg
    40621 agatgtctcc cagacggttt ccggaagagg cctgttcaat cgccgcgtat aaactctgtt
    40681 cggtcacccg catcaaccgg gccgcctctt cgctcacccg gcccacggga agcgtaaccg
    40741 ccgcatcccc gtaaaagccc tgataataaa cgccgaagtc cagtccgaca atgtcgcctt
    40801 ctttcaagac ccggccggac ggcattccgt gaacgacttc ctcgttgacg gatgcgcaca
    40861 gggaataggg atacccccga taccctttga acgccggctt ggcgcctcgc ttttccgtaa
    40921 cgctttcagc gattttgtcc aattcccggg tggttacgcc cggttttatt ctttcccgaa
    40981 gaacgctcag gacttccgca acaatccggc tgctcgcttt agctttgtcg atctcgtccg
    41041 gctgcttcag aatgaccatg agcccctgct ttcaacactc cgtctatcgc ctgcgcgcca
    41101 tgtgtccttt tttcatgaac ccctcatact ggcgggtcag cagatgcgat tcgatctggc
    41161 tcgccgtgtc catggccacg ccgacgacaa tcaacaatga cgtaccgccg aaatagaaag
    41221 gcgtgttcag ctgcgagatc agcacgctgg gcagcacgca gatgatggaa acataaaccg
    41281 ccccgctgaa ggtaagcctt tccagaattt cttcaatata ttcggcggtt ttctttcccg
    41341 gtcggattcc cggaacatat ccgccgaatt ttttcatgtt ttccgccatg tcgtccggtt
    41401 tgatcgtcac ggccgtgtaa aagaaacaga agaagaaaat caagccgaca aacaggattt
    41461 catagcccaa tcgcccgggc atcaggtaac ccgagatcgt cttcattaaa cccgccggcg
    41521 caaagttggc gatggtcgcc ggaaacatga tcaccgaaga cgcaaagatc ggcggaatga
    41581 cgcccgcgga attgatcttc agcggcagat gggttgtctg tccgccgtac atcttccggc
    41641 ccacaatcct tttggcgtac tgcaccggaa tccggcgctg cgccgcttca acaaaaacaa
    41701 tcgcgccgat gacggcgatc atgaacagcc ccagcagcac cagcaggaga aaatgcagtt
    41761 cgccggtcgc atacaggcgc caggttttgc cgatggcccc gggcagattg cacacaatgc
    41821 cggcaaaaat gatcagcgag atgccgttgc cgatcccctt ctccgtaatg gtctctccca
    41881 gccacatcag aaacgccgtt ccggacgtca gggtgatgat cgtcatcagg ataaaccccc
    41941 agccggcgtg cagaacgatg ggggcgcccg tcgggctggc catctgctgc aacccccagg
    42001 cgatgccgaa tccctgaatg atgctcagcc ccaccgtgcc gtagcgggtg tactgggtga
    42061 ttttgcggcg gccgttctcg ccctctttgg aaagtttctc caggtgcggg atggccaccg
    42121 tcagcaagtt gatgataatg gacgaggtga tgtagggcat gatgcccagc gcaaaaatgg
    42181 aaaaagtgct cagcgcgccg ccggcaaaca aatccatcaa gcccagcatg gatccgcgcg
    42241 cctgctcgaa aaaagccaga agcgccgtat tgtcgatgcc gggcgtcgga ataaagacgc
    42301 cgacgcggta caccgccaga agaagcagtg taaacagaat tcttctctgc agctcgggta
    42361 ttttggaaac gccgcccagt ccctctaaca aatcagatta cctcttcaat cgatccgccc
    42421 gccgcggtaa ttttggcttt cgcggccgcg ctgatttttg ccattttcac ggtcacggga
    42481 aaatcgatgt ttcccttggc cagcagtttg attcccgcgc cctttttctt gaccaggccg
    42541 ctttttaaaa tggcctcttc gtcaattacg gcgcccttct caaatttcct gcacaaatcc
    42601 atgacgttca cgccgacata gctttcccgg aagggattgc ggaagcctct cttcggaagc
    42661 cgccgggaca tgggcatctg gccgccttca aatccgtcgc gcacatagcc gccggagcgg
    42721 gctttctgtc ccttgtgtcc tttgccggac gttcccccgt ggccggacgc gtcgccgcgg
    42781 ccgactcttt ttctcttttt ggtcgccccc aggggcgctt tcagagaact caaatccata
    42841 gccctactcc tttgactctt ccacagaaac cagatggatg accttgtgaa tcataccacg
    42901 aatctccggg gtatcggcca gagtcacggt cttgtttacc ttggtcagtc ccaggccgcg
    42961 catgattttt ctctgctttt ccggccgcgt gatgacgctt ttcaccattt tcaccttcaa
    43021 cattttaccc acggtcttcc tgcccccgtc tgcttttact tgccccgtcg cgcggaaatt
    43081 tccgccggat ccttcaactg acaaagcccg tcaacggttg ctttgaccag attgtgcgga
    43141 ttatgcgagc ccaggcattt ggtcagaata ttgtgaacgc cggccacctc cagcactgct
    43201 ctcaccgcgc cgccggcaat caatccggtc ccttccgacg ccggcttcag cagcactttt
    43261 cccgcgccga attttcccag aacctcatag ggaatcgtct tgttggccac cgcgactttg
    43321 accatgtttt tcttggccat ttccattccc ttgcggatcg cctcgggaac ttcgtgggct
    43381 tttcccagtc ccgcgccgat catgccctgg ccgtcgccga ccaccacgat cgcgctgaag
    43441 cgaaaacgcc ttcctccctt gacgaccttt gccgtacggt taatggcaat gacccggtcc
    43501 aggagctcca cttctttcat gtctctttta tcgatggatt cttctctgat catgagttcc
    43561 gttcacaaaa gtaattaaaa tttaaggccg ctttcccgtg ccgcatcggc cagggctttt
    43621 acgcgtccat gatagagaaa ccgtcctctg tcaaaaacga ctttctccac tcccagcgtc
    43681 tgcaggcgcg ccgcaatcag cttgcccacc tcccgggcca catccacttt cttgatcccc
    43741 ttgtgctttc cggccaactc cctgctcagc gtggaagccg tcgcaatcgt tgtgccctgc
    43801 gcgtcgttga tcacctgtgc ataaatgtgt ttgtccgacc ggaaaaccga aaggcggggc
    43861 cgctccgcgg ttcccgtcag tttgcccctg actctctttt ccctttttgc tcttacttta
    43921 agcgtctttt tggaagccaa tgttccacct cttttttgct gtgtttcccg aaacgattat
    43981 ttagcgcctg ccgccttgcc ggccttccgg cgaatatgct caccggcgta tttaatgcct
    44041 ttccccttat agggctccgg cttcttcaac gagcggatct ccgacgcgac gcggcccacc
    44101 agctctttgt cgattccgga tacaacgagg ttgacctgct tgtcgatcct gacctcaatg
    44161 cctttgggaa tgctgtattc aaccgggctg gaaaatccca ggaccagttt caggttcgaa
    44221 cccgcaagct ccgcgcggta gccgacgccc gagatttcca gtcccttctc aaaaccggcg
    44281 ctgacgccgg tcaccatgtt gttgaccagc gttctcgcca gcccatgata ggaccgtgcc
    44341 agacgatcat cggattttcg ctcaatgatc agctgatttt cctccatcgt catcgcaatg
    44401 ccgtccggca attttgaaga caaggttccc ttgggcccct ccactttcac gatattgccg
    44461 ttctggctga tcttcacatt tttcggaaac gcaattggtt tcttgcctat tctcgacatg
    44521 aatcagaact cccgtctacc ctctggaatt gaactaccaa accttgcaaa gcacttcacc
    44581 gccgacattc gactctctgg cttcctggtc ggtcatgatg ccgcgggacg tggaaagaat
    44641 cgaaatgccg aagccgttga gcaccttggg aatcttgtcg gccgaaacgt acacccgacg
    44701 gccgggtttg ctgacccttt gaatatcggt aatgaccgta tgtttggcat ccggatattt
    44761 gagcgtaatc ttgagcattg cacggccgct ttcatccttc acggcctcat aattatggat
    44821 atatccggcc gctttcagga ctttggcaat attcatattc atctgcgaca tgtaaacgct
    44881 gacattcttg aagcgggctt tattcccgtt gcgtatccgg gtcagcatat cggcaatagg
    44941 atccgtcatc cccatgctta ttctccttac acgatggtta ccaacttgat ttgatcacgc
    45001 cggggagctc gccctgtagg gacagctttc tcaggcagat tctgcacatt tcgaattttc
    45061 tataatacgc ccggggccgc ccgcaaatct gacagcggtt gtattcccga accgcaaatt
    45121 tcggtttcct ttttgccttt gcgattaatg acttttttgc cacgctacaa ttcctccgga
    45181 gcttcctggt ttaattctta aagggaacgc ccatcaggcg gagcagaacg cgggcttctt
    45241 cgtccgtccg ggccgacgtc acgatggtaa tgttcatccc cttgatcttt tcgattttgt
    45301 catactcgat ttccgggaaa ataatctgct cgtgaaggcc gatggaataa ttgccccggc
    45361 cgtcaaaagc gctggccgat atgccccgaa aatcacggac tttcggcagg gcgacgttca
    45421 ccagccgatc gaaaaactcg tacatcacat cttttctcag cgtcaccgaa cagccgatcg
    45481 gcatgccctg gcggagcttg aaggtcgcga tggatttttt tgctttggtg accaccggct
    45541 tctggcccgt gatcatcgcc atttcctgca cggcgccgtc aatgattttc acgttggaga
    45601 tggcctcgcc cagacccata ttgacgacaa tcttctccat tttcggaacc tgcatgggat
    45661 tcttgtagtc gaattccctg accagagccg gcactacggt tttcaaataa tattccttta
    45721 atcttgccat tttccctaac ctacatcgat gacgtcattg catttgctgc agatccgaat
    45781 tttcttgccg tcctcgagaa tgcggttccg aatccgggtc gcttccttgc atttgccgca
    45841 aataatgctg accttggaga catccatcgc cgcctctttt tccaggacgc cgcccttgct
    45901 tttttgatca ggccttttat gtctcttgat cacattgatc ttttcgacga tcactttgtt
    45961 tttgccgggt atcgctttca gaatcttgcc ggtttttcct ttgtccttgc cggccagaac
    46021 tttcaccaaa tccccttttt tcaatctgcc taaactcatt tttcccctct tcaaaaccgg
    46081 cttataaaac ctctggcgcc agagagatga ttttcataaa ctgttttgct ctcaattcgc
    46141 gggcgaccgg cccaaaaatt ctggtgccga tgggctccat ctgattgctg atgagaacgg
    46201 ccgaattgtc gtcaaacttg agatacgagc cgtcgggacg cctcacttcc ttgacggtgc
    46261 ggacgatgac cgctttcatg acatcgcctt ttttgacttt ggaattcggg atcgcctctt
    46321 tcaccgccac gacaatgaca tcccccagag aagcgaagcg ccgcttcgat ccgccaagca
    46381 ctttaatgca cgcgacccgc ttggcgccgg aattgtcggc aacatttaaa atggtctgca
    46441 tctgaatcat aaatcaactc caccctcaca acgggcctgc atgggccaag ccaggtttct
    46501 attttgcttt ctccaaaact tccagcatcc gccagcgctt gtcgcgactg atcggccgcg
    46561 actcaatgat gatgacggtg tctccggtgc ggcactggtt ttgctcatca tgggccttgt
    46621 atttcacatg ccggcggaca tatttgtgaa acaccgggtg cttcaccagc cgttcggctt
    46681 tcaccacaat ggttttgtcc attttatcac tgacgaccac gcccttaatg gtgcgtttgt
    46741 tgcttttttc agccatatta tcctgcggcc tccttttcct tcatcaccgt cttgatgcgg
    46801 gcaatgtccc tgcggaggcg gcccaggctg gcggtcgatt ccagctggcc cccggcatgc
    46861 cgaatcttca accgaaacag ttcttccgcc aactcacttt tttttgtctg aagctcggcc
    46921 acaccgagac ctctgatttc gctaattttc atcggatatt tcccttgaac aaaacttggt
    46981 cgaaatcgcc agtttatgcg acgccagacg aaacgcttcc ctggccactt ccttggttac
    47041 gccttccatc tcgtacagca cggatcccgg cttcacgacc gccacccatc cttccggcgc
    47101 tcccttgcct ttgcccatgc gggtttcagc gggctttttc gtaatggatt tgtgcgggaa
    47161 cacgcgaatc cagatcttgc cgccgcgctt cacataacgg gtcatcgcaa cgcgggccgc
    47221 ttcgatctgg cgcgccgaaa cccatccgca ttccgccgcc tgcaatccgt agtcgccaaa
    47281 ggcaatggag cctccccgaa tcgctttgcc gcccatgcga cctttctgca attttctgta
    47341 ttttacccgt tttggcatca acataatcta caactcccgt tcctcttctg ccccccgtcg
    47401 gggtcaggac tgtctctcgt tcttctgggg caaaacttca ccgtgaaaaa tcaaaacttt
    47461 gacgccgacc aatccgtagg cggtatgcgc ttcgataaag ccgtagtcga tatcggcccg
    47521 cagcgtatgc aggggaaccc tgccttcacg gtaccattcc gcgcgggcga tttcagcgcc
    47581 ccccagccgt ccggaacagc tgattttgat gcctttggcg ccgaacttca gcgcgtagcc
    47641 gacgcacttt ttcatggcgc ggcggaaggc aacccgtcgt tccagctgca tggcgacatt
    47701 ttcggccacc agctgcgcat ccgtttccgg cttgcggact tccagaatat tgatgataat
    47761 ttccgcctgc gaaaattttt caatttccgc tttcagcttt tcaatttccg atcccttctt
    47821 gccgatgatc actcccggtc gggcggcgta aatattcacc ttggccttgt tggccgcgcg
    47881 ttcaatctcg atgcgggaga tgccggcatg atagagcttt ttcttgaggt acttccggat
    47941 cttcaagtcc tcaagcagca tgccgctgta atttttttcg gcaaaccata cggactgcca
    48001 ctttttgatg tagcccaacc gtaatcctac cgggttaacc ttctgaccca atatcgttcc
    48061 tccttacctt tcatccacga ccacggtgat gtgggacgtt cttttcctga ttcccgccgc
    48121 tcttccctgc gcccgggccc gccaccgttt caaggacggc ccctggtcca caaagatctc
    48181 cttcacgtaa agcgtgttct cgtcaatatt cggattctgg gccgcgttgg cgaccgccga
    48241 tttgagcacc ttggccacga ttcccgccga atattttcgc gtgtacagca ggatattgcg
    48301 ggcctcctga actttcttgc cccgcaccag gtcaaccacc agccgaactt tctgcggcga
    48361 catccggata tattttgcga ctgcttttgc ttccatatct catccactcc aaacccgtcc
    48421 gcctgcgcga acgccctact tcttcacctt ggttttccgg tcgccggaat gggaataaaa
    48481 cgtcctcgtc ggcgcgaatt cgcccatttt atggcccacc atgttttccg tcacgtacac
    48541 cggaatgaat tttttcccgt tgtgcacggc aaacgtgtag ccgatcagtt cgggggtaat
    48601 ggttgaacgc cgcgaccaag tcttaatcac attcttgctg ctttcagcct ccattttccg
    48661 gactttggtc agcaaacttt cttcaatata aggacctttt ttgatcgaac gtgccacgtt
    48721 ttatttccct cttcttttca caatatattt atcggttttc ttgtttttcc gggttttgtg
    48781 tcccttggtc ggaacgcccc agggggtgca gggatgacgg ccgccggatg accggccttc
    48841 gccgccgccc atcggatgat ccacggggtt catgacgacg ccgcggacgt ggggacgctt
    48901 gcccagccat cgggtgcggc ccgccttgcc gatgctcacg ttttcatgct cgttgtttcc
    48961 cacctggccg atggtcgcca tgcattcgat gaagaccttg cggacttcgc cggacggaag
    49021 ccgcacctga gcgtaactgc cttccttggc catcagctgg ccgtaggcgc ccgccgagcg
    49081 gatcaactgt ccgccgcgtc cgaccttcaa ttccacattg tggatcagcg agcccagggg
    49141 gatgtatttc agcgggatgg catttcccgg tttgatgtcc gctttctctc cgctcaccag
    49201 cacatcgccg acatggatct gcgccggggc cacaatgtat cttttttcgc cgtcgcggta
    49261 attcaaaaga gcgatccggg ccgatcggtt cgggtcgtac tcaatggccg ccactttggc
    49321 cggaatgtcc gccttgttgc ggcaaaaatc gatcatccgg tacttgcgct tgtggccgcc
    49381 gccgatatgg cgcgccgtga tccgcccgta actgttgcgc ccgccggttc tcttgatcgg
    49441 cttcaaaagg cttttgacgg gctcggtgga ggtgatctcc gcaaaatcag aaacgctctg
    49501 gaacctcctg cccggcgatg tcggtttgta tttgatgatt gccatgtgct tagtccttca
    49561 ctcgttctat acgccttcgg cgacttcaat gcggctgcct tcggccagcg tcacgattgc
    49621 ctttttccag tcgcttttct tccccatcac gcggcccatg cgtttcttct tgcccagcac
    49681 atgaatcacg cggacctccg tcacttttac cttgaataat ttttccaccg cctggccgat
    49741 ttccaccttg ttggcccggc gatcgacttc gaagtggtat ttatttgccg cctcacggtc
    49801 gaggttgctc ttttccgtga ggagcatttt ccggatcacc tgatgcagtt ccattatgcc
    49861 agcaatgccc cctcgatctt ttttatggag ggttcaagaa ggaccagatg ctcataattg
    49921 agcacatcat agacattgat gccttccgac cgaatcattt tcacattttt gatgtttcgt
    49981 gatgatttca tcaaaacaac attatcctga tcaatcacaa aaagcgcttt tttcaggccg
    50041 aagagatcca ccactttctg aaacgccctg gtgctgattg cttccatcgg aaaatcgcgc
    50101 agaatggtca tcttttcttc tttcactttc atgctcaacg ccgagatcag cgcatttttg
    50161 cggacttttt tcgggacgct gtaagcgtaa tcgcgcggct gcggaccaaa cacaatcccg
    50221 ccgcctctcc agatcggcga gcgcgacgtt cccgcgcggg cgcggccggt gcccttttga
    50281 cgccagggct tctttccgcc gccgctcact tcgtttcttc ccttggtggc agaggtgccc
    50341 gagcgccgcg atgcccgctg catcttgacg acctcataga tcgttgcttc gttgatgtcc
    50401 gcgccgaaaa ccgcgtcatt caattcgacc tcggcaacct tctttttttc aatatcaaaa
    50461 acatctgcga ctgccatgtt gacaactcca ctgtcttacg cgcttttttt cctggcctgc
    50521 gaaatgaaca catagccgtt tttcccgccg gggacggaac ctttcaacaa caacaaattg
    50581 cggtcggagc gcacctgcca gacctgaagg ttttgaacgg ttttccggac gtttcccatc
    50641 tggccgccca ttttggttcc cttgaagacc cgggacggat ccgcgctggc gccgatggaa
    50701 ccgggggccc gatgaaacat ggagccgtgc gtcgcccggc cgccgccgaa tccgtgccgc
    50761 ttcacaaccc cttgaaatcc tttgcctttg gaagtgccca ccacatcgac aaaaccgcct
    50821 gcctggaaga tgtccgcgga gatttcctgc ccgacttcgt acgcgccggc cgcggcggga
    50881 acttccctta aaacacgaaa gtatccttta ttggccttat tcaaatggcc ctgcagcggc
    50941 ttcgtgacat tcttctgctt gatccggccg taggcgattt gaaccgcgtc gtagccgtcc
    51001 ttttctctgg tcttcacctg aacgaccacc gacggctcga cctcaatcgc cgtcacgccg
    51061 accgcggcgc cgtcttcggc aaatacctgc gtcatcccca atttttttcc aagaattccc
    51121 ttgttcatca tcatccaact tttcaatgaa ataaagccgc gaacctcaac gttttggaaa
    51181 aatggcaatc ccaaaggcca ctttccaccg cctttatttt ccgcctacgc ttttatctcg
    51241 acatccactc ccgccgatag atccagcttc atcagcgcat ccaccgtggc ttgcgtcggc
    51301 tcgacaatat caatcaatct tttatgtgtg cgaatttcga actgctcccg ggatttctta
    51361 tccacgtgcg gtgaacgatt gacacaatat ttattgattt ccgtaggaag aggaatggga
    51421 ccggcgacgc gagcgccggt cctctttgca gtctctacaa tttcttcgac agaacggtcc
    51481 agcagtttgt agtcgtaagc tttgagacga attcttatct tctgttcttt catggatcgg
    51541 ttaccctgcc atttctacgc aatgaccttg cttacgacac ccgcgcctac ggtcctgccg
    51601 ccttcgcgga tcgcgaatcg cagctgttct tccatggcga tgggcataat cagttcgacc
    51661 gtcattttaa cgttgtcgcc gggcatgacc atctccacgc cttcgggcag cgtcgcgacg
    51721 cccgtcacgt cggttgtccg gaaataaaac tgcggacgat acccggaaaa gaacggcgta
    51781 tgccggccgc cttcttcctt cgtcaggacg taaaccgccg cttcaaattt ggtgtggggc
    51841 gtaatcgaag cgggtttggc cacgacctgc cctcgttcca cttcttcacg cttgatgccg
    51901 cgcagcagca acccgacgtc gtcgcccgcc cggccttcgt ccagggtttt gcggaacatt
    51961 tccacgcccg tcacaaccgt tttgatcgtg ggacgaatgc cgatgatttc aacttcttca
    52021 cccaccttga tgatcccgcg gtccacacgg cccgtcacaa cggtgccgcg gccggaaatg
    52081 gtgaaaacgt cgccgaccgg catcaggaac ggtttatcgg tgtcgcgctt cggctcggga
    52141 atgtactcat ccaccgcttt catcagttct tcgatgcatt tcacatcggg agacgccata
    52201 tcctcggctt ccaacgcttt gagcgccgaa ccgcggatga tgggaatcgc gtcacccggg
    52261 aattcatact gactcaggag ttctctcaat tcgagttcaa ccagatccaa caattcggga
    52321 tcatcaacca gatccacttt attcaggaaa acgacgacgt agggaacctg cacctgacgg
    52381 gcaaggagga tgtgctcgcg ggtctgcggc atcggaccat cggaggcggc taccaccagg
    52441 atcgttccgt ccatgtgggc ggcgccggaa atcatatttt tgatatagtc ggcgtggccg
    52501 gggcaatcca cgtgcgcgta atgtcttttt tccgtttcat actccacgtg ggaaatgttg
    52561 atggtaacgc cgcgttcctt ttcttccgga gcattgtcaa tcgaatcaaa agcccggaat
    52621 tccgcgaagc cttttttcgc cagatatttg gtaatggcgg ccgtcaacgt cgtcttgccg
    52681 tgatccacgt gaccgatggt ccctatattt aaatgcggct ttgtcctttc aaattttttc
    52741 tttgccatct cacctttcct ccttaacttt gaatggttgg tttccttgtc gtttgatgaa
    52801 tttctttaaa atttataatg cgcaaaggcc ttgttggcct ccgccatttt atgcaccgct
    52861 tctctcttct tgatggcgcc gccccgattg ttggccgcgt cgatgagctc cgccgccagc
    52921 ttttcccgca tggttttctc ggtgcgctcc tgcgcgttgg agatcagcca gcgaatcgcc
    52981 agcgccgtgc gccgggcggg aacgacttcc gtcggcacct gatacgtgga tccgcccacc
    53041 cgccttgatt tgacttcaat caccggtttg acgttattga cggctttctc gaaaaactcc
    53101 accggctgtt ccttgacgcg cttttcaata atgtcaaaag caccgtatag aatgctttcc
    53161 gcgacgcttt tctttccccg tttgagcaaa gagttcacaa acttggcaac cagtttgttg
    53221 ttatatttgg gatccggcag aatctccctt ttttcaattt ctcttcttcg tggcatgttt
    53281 tttcagtcct ttatctaact gggctttttc gcaccgtatt ttgatcgact ttgcttgcgg
    53341 tcggcaacgc cgacggcatc cagcgtaccg cgaacaatat gatagcgaac gccgggcaaa
    53401 tccttgaccc ggccgccgcg aaccagaacc accgagtgct cctgcagatt gtggccgatg
    53461 ccgggaatat aggatgaaac ctcatagccg ctcgtcaggc gaaccctggc caccttgcgc
    53521 aaagcggaat tcggcttctt gggcgtcgtc gtatagaccc tcacgcatac cccgcggcgc
    53581 tgcggcgatc cggccagagc cggggcgttt gtttttctct tgatctgggt tctgcctttg
    53641 cgaaccagtt ggctaattgt cggcatttct cctcccgaac gtttttctgc attttgctgg
    53701 cagcagcaaa tctgtggttg ctatcaattc aggatgcttc tgtcaagcaa tttcttgcct
    53761 tttggactca tttattccga ggccgcttcc gcttcctcct cttccacctt aattcccaat
    53821 ttgcgataca tgaccaggcc ggttccggca ggaatcaggc gccccataat gacgttttcc
    53881 ttgaggcccc ggaggtagtc cgttttcccg gaaagcgacg cttcggtcag cacccgggtc
    53941 gtctcctgga aggaggcggc cgagataaag ctctgggtgg aaagcgatgc tttcgtaatg
    54001 cccagaagca gcggatcgcc gatggcgggc ttcccgccct gagccatcac tttgtcattt
    54061 tcttcctgga aacggtagcg ttcgacctgt tcatcggcga taaaagtcgt gtcgcccgga
    54121 tcggtgatcc ggactctgcg caacatctgg cggacaatga cttccatatg cttatcatta
    54181 atcttcaccc cctgcaaacg ataaacttcc tgaacttcat cgaccagata ccgggccagt
    54241 tccttttcgc ccttgatggc caggagatca tggggattgt tgacgccgtc catcaacgcg
    54301 tcgccgggag ccacacggtc tccctcctga acactgatat gcttcccctt ggggatcaga
    54361 tattcttttt cttcgccgat ttccggcgtt acgacaattt tgcgcttgcc cttggcgtct
    54421 tttccgaacg tgacggtgcc ctcaatttcg ctgatcactg caaaatcctt tggcttgcgc
    54481 gcctcaaaga gctccgcgac tctgggcaaa ccacctgtaa tgtctttcgt tttggttgtt
    54541 tcccggggaa ttttcgccag aatgtcgcct gcccggacct gatcgccctc ggaaacaaca
    54601 atgttgacgc caaccgacag gaaatagcgg gccgcggcat tcgatccggc gattttagcc
    54661 gttttcccct tgctgtcctt gatggacaca cggggccgaa aatcgccgct tttaaattcg
    54721 acgatgacct tccttgacag accggtgact tcatcgacct gttcctgcat ggtttttcct
    54781 tcaataatat cgccgtattt gatggttccg tccacttcgg ccagaatcgg tatggaaaaa
    54841 ggatcccact cggcaatcaa ctgcccgcgc ttcacggagc tttcgtctcc tactttaatg
    54901 tgggcgccgt agggaacggg gtatttgccg cggctgcggt tctgctcatc cagcacatgg
    54961 atttcgccat gacggttcat caccacataa tcattttccc tggttttggc tgtgttcaga
    55021 ttcacaaatt ttacccggcc gtcatggcgc gcctccagcg tggagtgttc gtcaaatttt
    55081 gccgtgccgc cgatatggaa tgttctcatg gtcagctgcg tgccgggttc accgatggac
    55141 tgggcggcaa cgatgccgac cgcttcaccg atgttcacca ggtggccatg ggccagatcg
    55201 cgtccgtagc acatggcgca tacgccgtgt ttggcccggc aggtcagtac ggagcggata
    55261 ttcacccgct caatgcccgc gcggtcgatt ttttccgcca gggcttcgtc gatggcctca
    55321 ttggccttta cgatcacttc gtcggtgaag ggatcccgga tttcctgcag agcgacgcgg
    55381 cccagcacac gctcgccggc cgtttcaatg atttcaccgc cctccatcag cgcggataca
    55441 tagagtccgt caaccgtttc gcaatcctgc tcggtgatga tgcaatcctg cgccacatcg
    55501 accagtctcc gggtcaggta gccggagttc gccgttttca gggccgtatc cgccagtccc
    55561 ttgcgggcgc cgtgcgtgga aatgaagtac tgcaaaaccg tcagcccttc acggaagttg
    55621 gccgtaatcg gcgtttcgat gatttcgccg gagggcttcg ccatcaggcc gcgcatgccg
    55681 gccagctgcc gcagctgctg cccggagccg cgggcgccgg aatccgccat catgtaaatc
    55741 ggattgaagc tttcaattct gcgctttttg ccgtccgcgc ccgccacttc ttccgtactg
    55801 atgccggtca gcatttcgga agcgatcttt tccgtcgcct gcgcccagat gtcaatgacc
    55861 ttgttgtatc gttcgccgtc ggtaatcaaa ccgtccatgt actgtttctg aatcttcagc
    55921 acatccttgt ccgcctgcgc cacaatgttt ttcttgtttt cgggaatgat catgttgccg
    55981 accgcaaagg acaggccgga aatcgtcgca tatttgaatc ccagatcctt gaggcggtcg
    56041 gccaggagca ccgtggtttt atcgctgcaa acccggtaac agtgatcgat caggttggcc
    56101 agctccttct tgttcatcag cttgttgatc gcgtcaaaac tgatttcctc gggcagaatc
    56161 tcaaaaagga caatgcgtcc ggccgtcgtt tctttgagct ccgattcaat gcggactttg
    56221 atccgcgcat gcaggtcaat cgctcccgcg tccagcgccg agcgcacttc gtccggaccg
    56281 gagaagatca tgccttcgcc cttcacatcc ttcttggcgc gggtcagata gtaaattccc
    56341 agaacgatgt cctggctggg aatgataatc ggcttgccgt tggccgggga aaggatgttg
    56401 ttcgtggaca tcatcagaac gcgcgcttcg gtctgcgcct cgatggacag cgggacgtgg
    56461 acggccatct gatcgccgtc aaagtccgcg ttgaaggccg tgcaaaccag cgggtgcagt
    56521 cggattgctt ttccttcgat cagcaccggc tcaaacgcct gcatgcccag acggtgcagc
    56581 gtcggcgcgc ggttcagcat caccgggtat tcccgcacga cttcgtcaac ggcgtcccag
    56641 acttcggagg tttcccgctc caccattttc ttggcgcttt tgaccgtcgt cacatagcct
    56701 ttttccagca ggcggttata gacaaagggc ttgaaaagtt cgagcgccat ctttttgggc
    56761 agcccgcact gatgcaaccg taaatcgggg cccaccgtga tgaccgaacg gccggaataa
    56821 tccacgcgct ttcccagcag gttctggcgg aaacggccct gcttgccttt gagcatgtcc
    56881 gacagagagc gcagcggccg tttgttggtt ccggtaatcg cgcgtccccg gcggccgttg
    56941 tcgaagagaa cgtccacggc ttcctgcagc atgcgctttt catttcggat aataatttcc
    57001 ggagcgttga gctcctggag ccgtttgaga cggttgttcc ggttgatcac gcgccggtac
    57061 aggtcattga gatcggaggt ggcaaaacgg ccgccgtcca gcggaaccag aggccgcaaa
    57121 tccggcggaa tcaccggcag aacggtcaac accatccact ccggcttgtt gcccgatttg
    57181 cgcagcgcct cgaccacctt gagccttttg acgatttttt tctttttggt gtcggagatg
    57241 gacgtcacca cttccgcccg gagttcttcg tagagcttgt ccagatcgat ttcttccaga
    57301 agctgacgca cggcctcggc gccgatgccg gcgacaaacc cgttctctcc gtactgctcc
    57361 ttcgcttcga tgtactcttc gtcggtgagg agctctttct ttttcagcgg cgtgtctttc
    57421 ggatccagca caatccagga ttcaaagtag agaacttttt ccagctcctt gagcgtcaga
    57481 tccatcacat tgccgatgcg gctgggcagg cttttcagaa accagatatg cgcaaccggc
    57541 gtcgccagcg taatgtgccc catccgctcg cggcgcacct tggactgaat cacttcgacg
    57601 ccgcattttt cgcagaccac gccgcggtgc ttcatccgct tgtagcgccc gcaaagacat
    57661 tcatagtctt tgaccggccc gaagatcttg gcgcagaaca gaccgtcgcg ctccggcttg
    57721 aaggtccggt aattgatcgt ctccggcttc ttgacctcgc cgttggacca ggaaaggatc
    57781 ttttccggcg aggcgatcga aatcttcatg gcattgaagc gaatcggatt tttgggtttt
    57841 tcaaaatagc tgaaaacgtc ttccacagat ttttctcctg ttttttaaaa caaaattgcg
    57901 gggttactct tcctcgacca gttccacgtc cagtccgagg ctctggagtt ccttcaccag
    57961 aacattgaag gattccggca gacccggctc gaaggcatgt tcccctttga cgatggattc
    58021 gtaaatccgc gtgcgtccgg cgacgtcgtc ggacttgacc gtcaaaaatt cctgcagcgt
    58081 gtaggaagcg ccgtaggctt ccatggccca gacttccatt tctcccaggc gctggccgcc
    58141 gaactgggcc ttgccgccca gcggctgctg ggtcaccagg gaatagggcc cgatcgaacg
    58201 ggcatgaatt ttgttgtcca ccaggtgatg cagcttcagc atgtagataa tgccgacggt
    58261 gacttcctga tcaaagggct cgccggtccg gccgtcgaac aggaccgcct gtccgctttc
    58321 cggaaggccc gccgtgtcca gcaatttgcg gatttgcggt tcgggacagc cgtcgaacac
    58381 ggggctggcc accagcatgc ctttcttcag gcggccgaca aaccgcttga tgtcttcggc
    58441 ggacgctttt tccaaaaact cgtcaaagtc cggagaagcg taggtttttc tcaattcact
    58501 tttgatctga ttgaggttgt tgttcttttc cagcaattca ttcaggcctt cgccgatgga
    58561 ttttgccgcc cagcccaaat gggtttcgag gatctggccg acgttcatgc gggaaggaac
    58621 gcccagcgga ttgaggataa tatcgacggt ggagccgtct gaaaaataag gcatgtcttc
    58681 ttccggaaga atcctggaca gcacgccctt gtttccgtgg cggcccgcca ttttgtcgcc
    58741 cacggacaat ttccgcttga tggcgacata gactttcacc attttaatga cgccgggcgg
    58801 cagctcatcg ctggcctgaa tcttctccag cttctcctca aagcgggcct tcacaaaatc
    58861 gatttttctt tccatcacgg aaagcaggtt cgccagcttt tcttccgaac cgtcgtgctc
    58921 cgaaagcgtt acatccttcc agtacatgaa aggaatttcc tgccaggcct cacgggtgat
    58981 tttttcgccc ttcttgagaa gggttttctt tttcacgtca acgatcttgc ccgccgccgt
    59041 tttgccgacc accagcttgg cgatgtcttt cttgatggat tccgccagaa tccggatttc
    59101 atcgtcgcgg tccttcgtca gcttttcaat cgcttcttct tcgatcgccc gggagcgcag
    59161 atccttgtcg gcgcctttgc gcgtgaaaat cttggcgtcg atcaccgtgc cttccacgcc
    59221 gggcggaacg cgcaaagagg tgtcccgcac atcgctggct ttttctccaa aaatcgcccg
    59281 cagcagtttt tcctccgcgg agagctgcgt ttcccccttg ggcgtaattt tgccgaccag
    59341 aatatctccg ggtttcacgg acaccccgag gcgaatgatg ccgctttcgt cgaggttccg
    59401 gagcgcttcc tcgccgacgt tgggaatgtc gcgcgtgatt tcttccttgc ccagtttggt
    59461 gtcgcgggcc atggtttcaa attcttcaat atgaatggag gtgtaaatgt cttccttgac
    59521 aatccgctcg ctgaccagaa tcgagtcttc gtagttgtac ccgccccagg acatgaacgc
    59581 gaccatcacg tttcgaccca gcgccagttc gccggtgtcg gtggcggggc cgtcggccaa
    59641 aatatctctc ttccggacgc ggtcgcccgt ggaaataatg ggcttctggt taaagcaggt
    59701 gtcctgattg gagcgctgat atttggacag tgtgtagata tccaccccgg tgtccagacc
    59761 gtcttcttcc ggcttgtcgg cgcgaaccac gatgcgcgaa gcatccacgc tttcgacgac
    59821 gccggaccgt ttggacacta tgacggcgcc ggaatcgcgg gccaccaccg cttccatgcc
    59881 cgtgccgacc accggcactt cggggcacat gagcggcacg gcctgacgct gcatgttgga
    59941 gcccatgagc gcgcggttgg cgtcgtcatg ctccagaaac gggatcaggg ccgccgccac
    60001 ggaaaccagc tgatcggggg ccacgtccat catcttgatg tcttccggca tggccgaaac
    60061 gaagtcgccg cctttccgga ccgaaatcag ttcttcctca aattttccct ttttatcgag
    60121 cggccggtcc gccggggcaa tgagctggtt ttcctcttcg atagccgtca tgtatttgac
    60181 atcattgagc actttgccgt tctcgaccag gcggtacggg ctttcgatga agccgaattc
    60241 gttcacgcgc gcgtacgtgc tgagggagac gatcaagccg atattcgggc cttccggcgt
    60301 ctcgatcgga cagatgcggc cgtaatgcgt cgaatgaacg tcgcggacct caaacccggc
    60361 gcgctccctt gtgaggccgc cgggccccag cgccgacagg cggcgtttgt gcgtgatttc
    60421 cgaaagcgga ttcgtctggt ccatgaactg ggagagctgg ctggagccga aaaattcatt
    60481 caccacggcc gacaccggtt tggcgttgat caggtcgtgc ggcatcatgg tttcgatgtc
    60541 ctgaaggctc atcttttcct tgatcgcccg ctccatgcgc accaggccga tgcggtactg
    60601 attttcaatc agttcgccga cggagcgcac ccggcggttc cccagatgat caatgtcgtc
    60661 cacgctgcac tcgcccacgc cgtttttcaa atcgatcaga tatttgacgg aggccagaat
    60721 atcttcgttg cgcagaacgg tcagatccgt cggcacattc aacccgagct tatagttcat
    60781 cttggcccgg ccgacatccg acaaatcata ggtttccagt tcaaaaaaga ggctgttgaa
    60841 aaacttcttc gcggtctccg gagtgggcgg attgctgggc cggagcctgc ggtagatttc
    60901 cataatcgcg tcttccgcgg tgtcgatgcg gtccatgagc agcgtgtcgc gaatggaggc
    60961 gttgtccagg tcttcatcga tatggatgaa gttcagttcc ctgaccccct tctcctgggc
    61021 aatctcaagg ccgttgaagg gaagctcttc gttgcagcgg aaaagaattt cgcccgtcga
    61081 gggatcctga acatccgagg aaagaatttt cccggtcaaa tccgcttcct tgatcgccac
    61141 gcgcttgatc cccgcgtcca tgatcttctt gagcaggggg cgggtgagct tgcgcccttt
    61201 cttgacgatg acttcatcgc ttttcgggtc tttgatatct tccggcgcct tctgcccggc
    61261 cagggattca tcaaccccga agtagatctt ttccccttcg agggtaattt tttcaatgtg
    61321 gtagaaatag ttcagaagaa attccgtcga attgcccatg gctttgagca gaatcgtgac
    61381 gggcatcttg cggcggcggt cgatgcgaac gtacagcaga tccttggaat cgaattccag
    61441 atccagccag gagccgcgga tgggaatgac gcgcgccgaa taaatcagct tgccggaggc
    61501 gaccgttttt cccttgtcgt gatcaaaaaa gatcccgggc gaccggtgca gctggctgac
    61561 gatcacgcgt tccgatccgt tgacgataaa cgtgccgttt tccgtcatca gcggaatttc
    61621 accgaagtaa atttcctgtt ccttaatgtc gcggatcggt ttggtctcgg gcgtttcacg
    61681 gttgccccgg ccgtcgggca gccggtcggt gtcaaacacc atcagacgga ccacgatttt
    61741 caaaggagcg gcataggtca tgcctttttg aatgcactcc ttcatgtcgt accggacatc
    61801 gccgatcttg tagctgacaa attccaggga gcattttccg ctgaagtccg aaatagggaa
    61861 aacgctctta aaagccccct gcaggccgac attgcgccgt ttggccaggg gcacgtcggc
    61921 ctgcaggaac ttttcgtagg accgtgtctg gatctcgatc agattgggaa tgtcctgtac
    61981 tttttttacg cgaccaaaat tttttctaaa gtcacccatg gtatccttga attcaatggt
    62041 ggagtgaaaa ttaccttatt taatctcgac cgttccgccg acttcctcga tcttcttctt
    62101 gatttcggcg gcttcctctt tggaaacggc ttctttaatc ggtttgggca cgccttccac
    62161 cagatctttg gcttctttca ggcccaggcc ggtgatggcg cgaacgactt tgatgacctg
    62221 aatcttctcc gcgccgaatc ccgtcagaac ggcgttgaac tccgtctttt cttccgctgc
    62281 aggcgcggcg cccgcggcct ggccgggcat cgccacggcg gccatcatgg gagcggcggc
    62341 ggacacgccg aatttttctt ccagttcctt caccagattg gaaagctcca gcacggtcat
    62401 gttttcaata aatttaacga catcttcttt cgtgatttga tcagccattt ttctattcct
    62461 ctttcgtatg gttttatgag tttaacgttt gctttttctc gctgtaggca tggagcacct
    62521 gcacaaagct tcggggcacc gcgctcaaaa ccgtaaccag cgatgtcggc accgccgcca
    62581 tgaccgaaac cagtttgccc agaagaactt ctctgccggg caaatcggcc agcgcggtaa
    62641 gttccgcttt cgtcagcatc ctgccgtcca gcacgccgac tttgatatcc agttcaggat
    62701 tttttttggc aaattccatc agcgccttcg tcggcgccac cggatcttta tagctcaaaa
    62761 caacggcgat gggatatttg aaatgatccg ccagaacagc aaagccggtt tctttcgaag
    62821 caatgcccag cagggtgttt ttgacaacct tcagctccgc atcgtttttt cgcaatgcat
    62881 tccgcagggc ttccattttt tcaacgttca tggcgttgaa actggtcaag acacccagat
    62941 gggccttttt gagcttttcc tgaagctcgg atacaatttg ttcctttgtt ctccgatcca
    63001 atttatcagc ctcctttcat gatgattttc tgcttgaggc cgaaaaacca agccgcacac
    63061 gaaatagaaa acggtcgatc cgcctttccg atccccgttt ccgggaaaat ggattacccc
    63121 gaagcctctt gaggcaatga ttttccactc tgcgggtctc ggcaggccct tgtccgttta
    63181 aacttccgta cctgctgtct tcgacctgat actgcttgca ccgtaagcgg acgctgcgtt
    63241 ttggggaatg cggcatccgc gaattttttc ctcagcttac gctcttgata tccagcggat
    63301 caaccttcac ccccgcgccc atcgtgctgg aaatcgcaat gcttttaata taggttccct
    63361 tgctggatgc gggtttcagt ttgatgatgg tctccagaag cgccgtgatg ttttcataaa
    63421 gtttttcagc gccgaaagac actttgccca ccggcgaatg aacgatgccg gctttttcca
    63481 cgcggaattc caccttgccg gccttcagtt ccctgaccgc gttggcaatg tcaaaggtca
    63541 ccgtccccac tttggggttg ggcatcaggc cgcgaggccc cagaatcttg gcgattttcc
    63601 ccacggaccc catcatgtcc ggcgtggcaa tcacgcgatc aaactccagc cagccgcctt
    63661 tgatcttttc aatgatttca tcgtttccaa ccagatccgc gccggcttcc agggcttctt
    63721 tttccttgtc gcccttggca aaaaccaata cgcggacggt ttttcccagg ccgttgggca
    63781 ataccacgct gccccgaacc atttgatcgg cgtgcgccgg gttgacaccc agacggacgg
    63841 cggcgtccac cgtctcatca aatttgactc ccgccatgga tacgaccatt tccgtagcct
    63901 ctttgagcgt ataacgcctg gtcggatcga cctttttctt cgcagcgata atgcgtttgc
    63961 ctcgtcttaa catgatcaaa cctcttcctt gattaacctg ctatttctat tcccatggac
    64021 cttgccgtgc cggaaataat tctgaccgcg ccttccatat cgacggcgtt caaatcattg
    64081 aatttcagct gcgcaatgtc ctgaacctgt ttggccgtga cggttcccac tttttcccgt
    64141 ttggggtcgg cggcgccctt ggcgaccttg gccgcctgct tgagcagaac ggacgcgggc
    64201 ggcgttttgg taatgaacgt aaacgagcga tccgcgtaca ccgtaatcac caccgggaca
    64261 atcatgcctt cctggttttg cgtcatcgcg ttgtaggcct tgcaaaattc catgatattc
    64321 acgccgtgct ggcccagggc cggcccgatc gggggcgacg gagtcgcctt tcccgctttg
    64381 atctgcaatt taatgttcgc aatcactttt tttgccataa tcatcacctg aattgaaaat
    64441 ttttttaggc ctgtatctta cgccttatcg tttctcatcc ccgacctact gcccctgggt
    64501 gacctggatg aaatccagtt ccaccggcgt cgggcggccg aagatgctca caatcaccct
    64561 cagtttgctt ttttccggcc ggacttcgtc aatcacgccg tcgaaattgg tgaagggtcc
    64621 gtcgataatc ctcacgtggt cgccgacgtc aaacttgaat ttgggctttg gtttgagcgt
    64681 cccttcgtca atccggacct tgagattctc aacgtcttta tccggaatcg gggaaggctt
    64741 gtccttgctg ccgataaaac cggtcacctt cggcgtattc ttgacgatat gccagttttc
    64801 atcgctcatt tccatgcgca ccaggatgta accgggaaaa aatttgcgct tggaggtttt
    64861 cttttctccg gaaatcagct ccaccacgtc ttcctcggga atcagaatgt ccgagaaata
    64921 agcctgggcg ccggccagtt caatcctttc ctgaaggctc agcttgacct tgttctcaaa
    64981 tccggaatac gtatgaacga cgtaccattt aaaagccatg aagttaccct aaaatgaatt
    65041 tgaccagttt tgcgagaatc aggtctatca cgcccaggta aagggatgaa atcgcgacaa
    65101 tcaccatgac cacgcccgtg gaagccagtg tctgcttccg ggtcggccag gtgacctttt
    65161 tcaactctgt cttcgcctgg gcgagaaact gcaccgccct gtcgaacagc aacttgaatt
    65221 tttccatatc gtcggccttt gttttcggga aaaactggca ggccaggagg gacttgaacc
    65281 cccagcatcc ggatttggag tccgg
//
LOCUS       DAOSRM010000003        64714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_480_length_64714_cov_119.744196, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000003 DAOSRM010000000
VERSION     DAOSRM010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 64714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64714
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_480_length_64714_cov_119.744196"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1273
                     /locus_tag="PLA58_00730"
     CDS             59..1273
                     /locus_tag="PLA58_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOR61348.1"
                     /translation="MSTSFLYHVFGLVGYNYVKTQYQGNAIIFTISHKREKLQCPACK
                     SRKVILRGTIKRRFKAFRFGFKIIYLELEVQRVGCLECDSVRQVSLGFADPRFSYTYA
                     FERYALELSKHMTIQDVAKHLSVSWDVIKDIQKRYLTKRFSKPCLKHLKTIAIDEIAV
                     RKGHVYLTVVMDMDSGAMVFVGNGKGADALEPFWKRLKRAKAEIEAVAMDMSPAYLGA
                     VTEHLPKALVVFDHFHVIKLFNDQLSEFRRDLYHEAKDILQKKILKGTRWLLLKNPEN
                     LDSVKKEKDRLEEALQLNKPLSCAYYLKEDLRQIWLQKTKAKAERVLTDWIKRSASSG
                     IKMLMKFAKTLAAHRSGILAYYDYRISSGPLEGTNNKIKTMKRMAYGFRDMEFFKLKI
                     MAIHESKYALVG"
     gene            complement(1409..2038)
                     /locus_tag="PLA58_00735"
     CDS             complement(1409..2038)
                     /locus_tag="PLA58_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR61349.1"
                     /translation="MKDMGAKERREREKERRKNHILDTARELLLEKGLNATSINQIAR
                     RSELSIGAIYFYYKDKEEIFAALQVEGLELLYRAILAAIDKKATPEKKIRAIAQVYLR
                     FSEENKNYFDIINYFLASPGTIFSPVLKKEIDAHGNASIALLADTIREGIDRGHFKKV
                     DPRRHAFILWSTFNGVIQIKKLEKTILGKGEHQSLYMEALEHFLSGLRA"
     gene            complement(2035..2304)
                     /locus_tag="PLA58_00740"
     CDS             complement(2035..2304)
                     /locus_tag="PLA58_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61350.1"
                     /translation="MSESYQLIYGFVHCRGRTEYSAGEVDSKEEAEAWVKNHREGLLP
                     KIKIPTDDPIRYCRAAWCPFKKQQPWFDMRPKGDTQAAKMKKEQG"
     gene            complement(2319..3341)
                     /locus_tag="PLA58_00745"
     CDS             complement(2319..3341)
                     /locus_tag="PLA58_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOR61351.1"
                     /translation="MKAVVFRKDRGLVYEEVPDYTVAEDEVLVKTANTGFCGSDHSLV
                     AGGHLADGYILGHEISAVIVEKGAAADGPPVGTRVCIRPTYCGKCANCLSGRPHLCRV
                     HRRTAGIGDLPGGFAEYVKVYPQMLIPIPDGVDSRNAALAETFAAALHGINCTSDRKG
                     SALVMGGGPIGLCAVRLLKIFGYSPVVLFEPVASKRDIAVLYGADQVFDPLAKDADQK
                     AMAAAPGGYRKIFECSGVKANINRAVLLAEDAGEICIVSMIFSPVSLEAPFLVNLKEI
                     RLTASNSNTHAENIQCLNWMAQGKLDARPLISDYEPLSRLPDVYRERIDTGLAVKVLL
                     KIGEEF"
     gene            complement(3450..4748)
                     /locus_tag="PLA58_00750"
     CDS             complement(3450..4748)
                     /locus_tag="PLA58_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR61352.1"
                     /translation="MNNVTAPKEYKLSTRSTYYIFALLFLLYFFDYVDRMIVTSLFPF
                     IQKEWGLTDTQSGLLVSMVYWSIVAFVLPVSILVDRWSRKKTIGIMAFLWSMATLACA
                     FTKTFPQLLMTRAAIGIGEAGYAPAGTAMLSGLFPEEKRSRMMGLWNMSIPLGTAVGI
                     GLGGFIAAHWGWRHAFGVVAIPGAIVAILFFFVKDYKTVDLAKTDAACTTGPGKIKMG
                     KMDIVWEFLRTPSLIFTNLGFIGCIFANNAIMVWLPTYFHRTTGIPMSEAGMKVSMLM
                     ILAIIGLPLGGWLADMWFKKRVNARLLFPAITSILNALVIFTAFSFLEGKTQYFTLLG
                     MGVLASAFAPAAIIATQEVIHPGLRAISYSVCVIVQNLFGASLAPIAIGKISDLHGIE
                     AAMSIVPVFLVISSLLFFAGSFFYKKDLDKVEKITLECEN"
     gene            complement(4821..>6518)
                     /locus_tag="PLA58_00755"
     CDS             complement(4821..>6518)
                     /locus_tag="PLA58_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOR61353.1"
                     /translation="MDNVIGGKLVADALVERGVDYIYSLSGGHITPIYQHLENTPITL
                     FDVRHEQAAVFMAEAAARMTRKPAVAMVTAGPGFTNALSGIASARLSNAPVILIAGCV
                     GTEAMEKLDLQDMTQLPVIEPMVKKAFVCYVPERIPEYVDMAFRAAMSGRPGPVYLEF
                     PCNILNAKVDPAAVKKPRTTVVSKPVDRENTRKVVDLLKAAKSPVVIGGSGLWYSDAE
                     KEILEFVEKIGIPIYTAGAGRGVVPDTHPLCFEGSLAIRPGSAMISLMTTDLVLFLGG
                     RLSLFYIFGEIFPSTAKFIQVDIAADEIGRNRTIDLGIVSDIKAFLKELNAVIDDQKI
                     ADTLKKQFQPWVDTVRAAHEAGKKDAKTMWECETLPMHPMRLAKEVNEFMNREDDIVV
                     ADGGDTSTWMGMTRTVKKGGTYLDYGLYGCLAVGIPYANAAKLKNPDKRVLLIMGDGS
                     VGFNFMEFHTAIRKKLPIVVVIGNDQSWGMIAHSQTLRLGHNIPNGTELGWVDYHKMV
                     EVLGGFGALVEKPEDIKPALEAAFASGKTACINVKVDPSVISPGSVALANLGGYKCE"
     assembly_gap    6519..6618
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6797..7636)
                     /locus_tag="PLA58_00760"
     CDS             complement(6797..7636)
                     /locus_tag="PLA58_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR61354.1"
                     /translation="MIYNDLKGKTALVTGAGKKTGIGYAIAEKLASSGCNVIIADLGA
                     PPAPEGPPQPVVSEEMEAIAAELAKTYSIRTLAVAVDVADNAAIGVMVETIKKNFDHI
                     DILCNNAGASFGVPNTVLGYDEAAWMKTIDVNLFSVFRMSRAVFPLMAGKPASIINTA
                     SRAGKVPPIFNSAYAVAKAGVIMLTKVMAKELGGSGIRVNAICPGQIATDLEKWRFGL
                     EASFFGTTIEEREKEMCKTIPLGYIGLPSDAGDLVAFLASDASRYITGQAVNLDGGQC
                     MEL"
     gene            complement(7626..9389)
                     /gene="xsc"
                     /locus_tag="PLA58_00765"
     CDS             complement(7626..9389)
                     /gene="xsc"
                     /locus_tag="PLA58_00765"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HOR61355.1"
                     /translation="MKKIKMTPSEAIVETLLAEGVDHVCGIVGSAFMDMLDLFPTAGI
                     KFIPVRHEQSAAHMEDAYCRISGRCGVVTGQNGPGITNMVTSVAAANMGHTPLVIISP
                     SAGTPTIGWDGFQECDQMSVFRAITKAVVRVPHPKRAADCLRTAFRIAYADRGAVLYD
                     IPRDYFYGELEEKILKPSQYRVDARGCGSIEQLDRAAELLSRAKNPVIVSGRGVVDAD
                     ALEIVKAIAEHLTAPVAVTYLHNDAFYGNHPLSVGPIGYMGSKAAMKTLAKADVMLAI
                     GTRLSVFGTLPQYDINYFPENAKIIQVDINPKHIARTHPIEVGIIGDAKEASAEILKR
                     LKALDGKRKPDEKRLAAVARENKKWEKEIVDLAMVDGNPINPRRVLLEMSRALPDDAI
                     VTTDIGNVSSTANSYLKFNHPRRHIAALTFGNTGFAYPAALGAQIARPKAPVIAIIGD
                     GAWGMSLHEVSTAVEHHLPVIACVFRNMWWGAEAKNQVDFYNNRFVGVDIPNPESFVP
                     VARAMGANAMQVGRPQDIREAFQTCLKSRKPTVLEFLVDGKQLAPPFRKDALALPTRF
                     LPKYAHLDYRHWVDEGGTDDL"
     gene            complement(9481..11175)
                     /locus_tag="PLA58_00770"
     CDS             complement(9481..11175)
                     /locus_tag="PLA58_00770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR61356.1"
                     /translation="MKPKRVVIIGASACGAKAAARIKRLRPDYDVAVLDRSSYISYAA
                     CGMPYYLEGKVRRMDDLLKTTDGIVRDTQYFKDLKDIAVHTHVKACRIDRENRRIMAT
                     NTATGKSLTFDYDDLVLAMGAAPKMPPIPGASLQGVHHLTRMEDTEVIAREVQNREHG
                     RAVIVGGGFIGVEAAEALKAKKWEVALLERESQLFPGMIDFEIAAMIREHFYEQMVET
                     EFDAGILSFEGENGQVRKVITRHHSYSADLVIVAAGLAPETELAQAAGLERGETGALK
                     VNAQMQTSDPAIYAGGDLVENVHIVTGKPCYIPLGSTANKHGRVIADNICGIPSTFPG
                     VQGTFICKAFDYAVGATGLSEAQAKRMGIDAFAISVCGFDKAHYYPNSQIIALKLIVE
                     NGSHKLLGLQAVGKGDVARRIDVAATAIRLGATISDVDDLDMAYAPPFSPALDVFVTA
                     LNVAQNVKSGMMKPVTAQEAKKLLEEDDSVLMIDVRSDLEFRRAHISSPRVVNIPLDE
                     LRRRIQEVPKDKKIICVCLLGLRGFSAQRILEGAGYSDVRTLEGGIFLWPWKEDLD"
     gene            complement(11172..12332)
                     /gene="nrfD"
                     /locus_tag="PLA58_00775"
     CDS             complement(11172..12332)
                     /gene="nrfD"
                     /locus_tag="PLA58_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOR61357.1"
                     /translation="MLEKALKGNRGYWIWIACLILVATVGFFFYLRQLDYGLGITGMS
                     RDVSWGLYIAQFTFLVGVAASAVMLVLPYYLHDYKAFGRITVLGEFLAVSAVVMCILF
                     VFVDLGRPSRVMNVILHPQPKSILFWDMIVLSGYLLINIVTGWVILSCNRKEIPPPRW
                     VKPIIYLSIPWAISIHTVTAFIYAGLPGRGFWLTAIMAPRFLASAFASGPALLILVCL
                     ILKRWGCFDAGEKAIQTLAKIVAYALAVSIFFVLVELFTVFYSQIPEHMHHFQYLFAG
                     LDGRYNLVAWMWTFAVLSIAAFVILIFPGVRRKESWLALACVLVFVSLWIEKGLGLVI
                     TGFIPSPLGAITEYSPTGPEIAITLGVWAAGFLMLTLFYRIFTSVHFERENR"
     gene            complement(12340..13122)
                     /locus_tag="PLA58_00780"
     CDS             complement(12340..13122)
                     /locus_tag="PLA58_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR61358.1"
                     /translation="MTPNRRDFLKIAGASALTIMTPAIFVPAWAKNSIVMPPGQGLAP
                     QKALAAKRWAMAVDLTKCTANCADCIVACHAIHNVPEFGNPKDEVKWIWTEPFASVFP
                     GESHAYSGEKLQKMRPPVFCNHCENPPCVRVCPTKATFRRSDGIVMMDYHRCIGCRLC
                     MAACPYGARSMNYRDPRPFTAKINPDFPTRTKGVVEKCNFCEERLAKGTLPACVEACR
                     QKALTFGDADDPRSDIRRLLETRFSLRRRAHLGTGPQIYYLL"
     gene            complement(13119..13520)
                     /gene="dsrJ"
                     /locus_tag="PLA58_00785"
     CDS             complement(13119..13520)
                     /gene="dsrJ"
                     /locus_tag="PLA58_00785"
                     /inference="COORDINATES: protein motif:HMM:NF038038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrJ"
                     /protein_id="HOR61359.1"
                     /translation="MKNNKWGLILLGLAIFVIGVTFPFWYGKEKTAPPALSLETPEIA
                     GSADKKCVEDKAFMRANHMKLLNAWRDEAVREGKRIYTAKDGRTFEKSLTGSCIRCHS
                     NKEQFCDRCHNYVGAKPSCFDCHIVPAEVKK"
     gene            complement(13601..15205)
                     /locus_tag="PLA58_00790"
     CDS             complement(13601..15205)
                     /locus_tag="PLA58_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOR61360.1"
                     /translation="MSTAKIPEPDELMQIKYAPPQTAWMETPVDFRPGTWSYGAAAKN
                     LAALDMPNPRAWQPSDADWKLPENWRQIILDGMKERLDKYRSFRLFMDICVRCGACAD
                     KCHFYIGSGDPKNMPVLRAELIRSVYRRYFTASGRCFGKLAGARDLTVDVLKEWFYYF
                     YQCTECRRCSVFCPYGIDQAEITMIGRELLNLVGCNINWALEPAANCFRTGNHLGIQP
                     GGIKDMLEFLADDIEDRTGVRVEVPINKKGADILFVTPSADYFADPGTYTCMGYMMLF
                     HEIGLNYTFSTYASEGGNFGLFTSHEMIKRLNAKIYAEAKRLGVKWILGGECGHMWRV
                     IHQYMDTMNGPADFLEEPVSPITGTRFENAKSTKMVHITEFTADLIKNGKLKLDPSRN
                     DHVRLTFHDSCNPARAMGLFEEPRYIIQKTCDHFYEMAEDTIREKTFCCGAGAGLGND
                     ENLEMRMRGGMPRAMAVREVVKKHGVNRLGCICAIDRATLTTLMDYWVPGVSVMGVHE
                     LVGNALVMKSEKERSTDLRGEKIGGS"
     gene            complement(15208..16194)
                     /gene="dsrM"
                     /locus_tag="PLA58_00795"
     CDS             complement(15208..16194)
                     /gene="dsrM"
                     /locus_tag="PLA58_00795"
                     /inference="COORDINATES: protein motif:HMM:NF038037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrM"
                     /protein_id="HOR61361.1"
                     /translation="MRNLGLAFVAVLLLALPAAAAAAADMTALLVVLPYGVFVLFLGG
                     FVYRVVRWAKAPVPFRIPTTCGQEKSLPWIHNNPVENPAGPLGAIGRMAGEVFLFRSL
                     FRNSQTEIVDGRPVYGSAKWLWFFGLMFHVSLLIAALRHLRFFTEPVVPLATGLSALD
                     GFLEIGVPALYISDVTLLAGLVFLFLRRVVLPQVRYISLFSDYFALLLIAAIAATGLL
                     MRHFFPVDLNAVKTLAMGWVSFAPSAPADASILFYLHLCFVFVLIAWLPWSKLSHMAG
                     IFLSPTRNQINNSRSRRHVNPWNAPVKVHTYAEYEDEFRDKMKAAGLPVEKE"
     gene            complement(16200..>17534)
                     /locus_tag="PLA58_00800"
     CDS             complement(16200..>17534)
                     /locus_tag="PLA58_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOR61362.1"
                     /translation="DNWTFLQPLYHSLEYCVKCQICSDACPAYVASGRQDIYRPTMRP
                     ELLRRIISTYVKKEHPLLRKFKASNIELNAQALIRLAELAYRCTLCRRCAQVCLLGCD
                     NALISREIRKIFSQQMGIAPKEVHEKGSVQQIKVGSSTGMTPPALADILEFTEEEIAE
                     KTGKKIKIPVDKKGSDILLLHNAGEFLAWPENLEAFAILFDAAGIDWTLSSELVGYDA
                     VNYGVWYDDVQFARVALRHAQAAKNLGVKRISLGECGHAHKAMIVVADRVLTGELNIP
                     RESALPLMEKIVMSGRIKLDPGRNNFPVTLHDPCNMVRLMGIVEPQRRILRKIAPQFR
                     EMTPHGVENYCCGGGSGFAVISSNNFPDWRSSVSGRMKLKQTLDAFADAPSADVKKYV
                     CAPCSNCKGQYRELFNTYGVWEKSGIFYGGLVELIVNAMADLKQPFIQWEWH"
     assembly_gap    17535..17634
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17635..17956)
                     /locus_tag="PLA58_00805"
     CDS             complement(<17635..17956)
                     /locus_tag="PLA58_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61363.1"
                     /translation="MIRKTGIQDFTKPGGQVAELEPADWMKLPAPYDQMDKPLKKLTE
                     DQRKRMDATLDGVVAIGILPPSMPEEEKAFVDRFLAGVEKLFDAKDNWTFLQPLYHSL
                     EYCVK"
     gene            complement(17959..18903)
                     /locus_tag="PLA58_00810"
     CDS             complement(17959..18903)
                     /locus_tag="PLA58_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOR61364.1"
                     /translation="MGTLLIVLTYIAYVFIVAVYAIKVRKWLKLPVHIRWDLYPVIHE
                     EQYRYGGSYYEKPEWWTQPRPKNTGRSLWHALKDNFYMGEYFKRNPLYWLFLLPWHLG
                     FIFIILFHITCFFAATAMVGGVTIASDSPQLLGQIFYGILMAFAVIAFIGGTFGCIGM
                     PVIKLADRGLRSYAMPMNYFNYFFFLIVYASGLYSWLALDPTLAEYRMYWVGLITLTP
                     PDLNPATVFHIILFDIFLIYLPFTRSTHYITRLLAYFKIRWDDEPNFRGSMLEKQLND
                     LLAQKLSWDAPHILKGQSWVEAAISSGLPETKPETKKD"
     gene            complement(19072..19392)
                     /locus_tag="PLA58_00815"
     CDS             complement(19072..19392)
                     /locus_tag="PLA58_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOR61365.1"
                     /translation="MPQIELAGKTYEVDEDGFLQELDKWSEEFAEAYAHVEEIDGKLT
                     EEHWMVINYLRNYYQQFGIAPMIRKICKDLKIDMKKLYELFPTGPAKGACKLAGLSKP
                     TGCV"
     gene            complement(19516..20955)
                     /locus_tag="PLA58_00820"
     CDS             complement(19516..20955)
                     /locus_tag="PLA58_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOR61366.1"
                     /translation="MNQRRPRIVIAALRGGAGKTTLSVALAVALRHQGIQIAPFKKGP
                     DYIDAAWLAQAASGPAFNLDTYLMGSERVVQSFTRHAAKNGVSIIEGNRGLYDGVNSA
                     GQFSTAELAKLLAAPVILVVDCDKVTRTAAAMVLGCQALDPKVDIQGVILSRVNGNRH
                     SSVIRRSIEESTGLPVYGIVPRLPDIPFVQRHLGLIPPDESTGVQRALEKAGNMARDY
                     LDVSGLLAVARSAPSWKIGAFPKPARIRRGIAEPTTIGVIRDAAFQFYYQENIEALTD
                     LGARVIQISALENKVLPPVDALYIGGGFPETQAEPLAQNESFRRCVKESAERGLPIYA
                     ECGGFIYLGESLTIGHRCYPMVGALPVAFGMERRPQGHGYTTLTVDRENPFFPMGTTI
                     TGHEFHYCRILAREDREMHTAFSIRKGTGIDGAREGLCRKNILAGFTHVHALGAPEWA
                     NALIAKAQEYRRTKQQTSGALESGIHMGV"
     gene            complement(20969..21193)
                     /locus_tag="PLA58_00825"
     CDS             complement(20969..21193)
                     /locus_tag="PLA58_00825"
                     /inference="COORDINATES: protein motif:HMM:NF020263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory sulfite reductase D family
                     protein"
                     /protein_id="HOR61367.1"
                     /translation="MSDDIKTKVTELFREKSKGDKKMFYIRDVTKWLPNEDRHAIQNA
                     VKELLDEEVLKYWSSGSSTYIMLAEYFPKE"
     gene            complement(21197..22261)
                     /gene="dsrB"
                     /locus_tag="PLA58_00830"
     CDS             complement(21197..22261)
                     /gene="dsrB"
                     /locus_tag="PLA58_00830"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HOR61368.1"
                     /translation="MAARTDIGPPDYKTMLPPVIQRNYGKWLWHEILKPGVLMHVSQS
                     GEALYSVRAGSPRLVSTDHIREICDIAEKHCEGFLRFTSRHNIEFLLDDKDKIEPLIA
                     ELKQRGYPVGGTGHSISNIVHTQGWVHCHTPATDASGPVKALMDEIYDHFTNSKLPAH
                     VRIALACCLNMCGAVHCSDIAILGIHRTVPKTDNSRVPNVCEIPSTVASCPTGAIRGD
                     MKNKTVTVNDEKCMYCGNCYTVCPAMPIADPDNDGISIWVGGKVSNARSAPMFSKLAI
                     PFLPNNPPRWPEVVNAVKNIIEVYASHAKKHERVGEWIERVGWERFFSLTGIPFTDQH
                     IDDFDLATETFRTSAQFKWR"
     gene            complement(22263..23450)
                     /gene="dsrA"
                     /locus_tag="PLA58_00835"
     CDS             complement(22263..23450)
                     /gene="dsrA"
                     /locus_tag="PLA58_00835"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HOR61369.1"
                     /translation="MANTDTPLLDELEKGKWPSFVKDMKEAAQKNPMAKDLLHQLELS
                     YKDKIGHWKHGGIVGVKGYGSGVIGRYSDRPDLFPNVQAFHTMRVNHPTGWFYKTDTL
                     RKLCDIWDKHGSGLTNMHGSTGDAILLGTTTEHLQPCFNDLSDAGFDLGGSGSALRTP
                     SACVGPARCEWACIDSLDICYNVTMTYQDKMHRPMFPYKFKIKISACANDCVASIARS
                     DLSIIGTWKDAIQIDQKEVAAYAETINIQKEVCDLCPTRCMELNGRELKIYDEDCTRC
                     MHCINVMPKALRPGKERGASILVGGKAPIVRGALLSWVVVPFMKLEPPYDELKDLIER
                     IQDWWDEFGKSRERLGELIQRLGMRVFLKAVGLPAVPQMVKAPRTNPYVFFWPEDIKK
                     EVK"
     gene            complement(23833..23991)
                     /locus_tag="PLA58_00840"
     CDS             complement(23833..23991)
                     /locus_tag="PLA58_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61370.1"
                     /translation="MTETKIKEEMEKMEYEPLLPIEKKLIGWSLILGVVLLVVLVWVS
                     YTFFPAGH"
     gene            complement(24016..25461)
                     /locus_tag="PLA58_00845"
     CDS             complement(24016..25461)
                     /locus_tag="PLA58_00845"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOR61371.1"
                     /translation="MGNKQGLLKTEDWWSVWLGLFIFILSLGSLVGLDLLGWAVTPKV
                     WTAFSKSIAPASKAYAGLHPLISIILTYLAVMAVLLVGAKALGYNLKKFIYGFTVIFW
                     LTYICTIVGHYAVIAAQTPAEMKKFELDWSLKLTGEAGLILALLVGLFIGNFMPKFAD
                     SLKEAARPEWFIKTAIVIMGVGLGVKSAEQLGLATSMMFRGLCAIIEAYLIYWAVVYL
                     IARKFFKFSREWSAPLASGISICGVSASIATGGAIRARPIVPIMVSSLVVIFAVVELI
                     ILPFVATEFLSNQPLVAGAWMGLAVKTDGAAVASGQIVESLIYARNAAQGINYQPGWV
                     VGAATTVKIFIDIFIGIWAFILAVIWTTKINPKQGVEAKAGEIWDRFPKFVIGYFVTF
                     LIMLIIGLNWPDIKSQLKISAAESDVFRKLFFALTFFSIGLASNFKKLWEEGIGKLML
                     VYIIALFGFIIWVGLIISWIFFQGVVPPLVQ"
     gene            26049..26762
                     /locus_tag="PLA58_00850"
     CDS             26049..26762
                     /locus_tag="PLA58_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOR61372.1"
                     /translation="MIENLISNLSANLQGSVGLSFVAAYLGGVLISFTPCTYPLIPLT
                     VGFIGAQGSGSKLRGFLLSVLYVLGLSVTYAALGAAAAWSGRLFGQMQTTPLVYWIMA
                     NICLVMGLAMLDVFKISLPVPQKMAAYSGGGRKGFLPSFLLGAASGFVIGPCTAPVLG
                     VLLGFVALKTHVVMGIGLLFVFAFGMGTLLILAGTFAGLIAALPRSGRWMTMVTRAFG
                     VILIGAAEYFLYTAGTLSF"
     gene            26777..27283
                     /locus_tag="PLA58_00855"
     CDS             26777..27283
                     /locus_tag="PLA58_00855"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOR61373.1"
                     /translation="MYKNSIVLALVFFLVLAVVSASRGQYIPNAPFKPQVFAAPDFTL
                     KDLQGKTFRMSAQRGKPVLIFFGTTWCPGCRAEIPKYKKVYETYSPKGLEVIYINIME
                     PAKKVARFAQANALPYRTLLDEDGSVGTAYNAIGVPMMILVDKDGAVVKVGFSSSQMP
                     LDKVLPVK"
     gene            27363..27572
                     /locus_tag="PLA58_00860"
     CDS             27363..27572
                     /locus_tag="PLA58_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOR61374.1"
                     /translation="MTTSNWFAEFSKSETDRWIGGVCGGLGEHTPVPSWVWRLLFALL
                     AVGFGTGFLLYLLLWIFVPRKAQTG"
     gene            27620..28285
                     /locus_tag="PLA58_00865"
     CDS             27620..28285
                     /locus_tag="PLA58_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR61375.1"
                     /translation="MKFRDDIYIYEWANYFDNNCNSYYIGGGVQALIDPGLTRYLPDL
                     LNQMANDGIKKKDIRYVINTHSHPDHFQGSELFDQEKVKIALHRKEMDFLKGVGGELY
                     GLFGMTIPQMQINLQLEDGRLELGDQTFRVISIPGHTPGSIGLYWPAKKVLFCGDVVF
                     QQNVGRTDFPGGNGALLKRSIVALSDLDIDLLLPGHMGIIEGHDKVQDNFNIIIQNIF
                     PYI"
     gene            28349..30034
                     /gene="ettA"
                     /locus_tag="PLA58_00870"
     CDS             28349..30034
                     /gene="ettA"
                     /locus_tag="PLA58_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOR61376.1"
                     /translation="MANEGNKVIYSMIGVSKVYDKKPVLKDIYLSYFYGVKIGVIGLN
                     GSGKSSLLKILAGVDDEFLGKTILSPGYTVGYLEQEPRLDEDKTVRRIVEQGVQATMD
                     VVNEYNAVNEKFSEEMSEEAMAKLVDRQAALQEKMDALDAWDIDSRLEMAMDALRCPP
                     GDTPVKILSGGERRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLEHHLQKYEGTII
                     AVTHDRYFLDNVAGWILELDRGQGIPWQGNYSSWLEQKQNRLKSEEKSESARIKTLER
                     ELEWIRMSPKGRHAKSKARIKAYEALLDRDLERESGTREIFITPGPRLGDVVIEAEGV
                     SKGYGEKLLVEGMTFSLPPGGIVGVIGPNGAGKTTLFRMITGQESPDAGTIRIGETVK
                     LAYVDQSRDALDPEKNIWEMISDGQDIIPVGGRQINSRAYVSRFNFSGTDQQKKVGTL
                     SGGERNRVHLARMLKEGGNVLLLDEPTNDLDVNTLRALEDALENFAGCVVVISHDRWF
                     LDRICTHILAFEGDSKVLFFDGNYSEYEEDRKSRLGAAASQPHRIKYRQLTRR"
     gene            30109..30471
                     /locus_tag="PLA58_00875"
     CDS             30109..30471
                     /locus_tag="PLA58_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61377.1"
                     /translation="MKKIWTTLTAAVLLFSCLPPAQAQEYGKVRALQERAAYVTRQKN
                     DFVVRVLRSYEIPHEVNDQGVVVRINMGGRWMDVTSIEIVPVLREAEDQSRQVAAHEL
                     FFFTTDGILDVVSALTIR"
     gene            complement(30474..30797)
                     /locus_tag="PLA58_00880"
     CDS             complement(30474..30797)
                     /locus_tag="PLA58_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61378.1"
                     /translation="MPVYAQILLLVAGFMVFVLAAMYLTGLGLRRVCFKIIAELEEAG
                     AVKASRAMKLQDERKNFFRVGTGNLRPRALHLLISDKLVVRTSDGKYYLDQEKLAEMK
                     RTLGK"
     gene            complement(30856..31257)
                     /locus_tag="PLA58_00885"
     CDS             complement(30856..31257)
                     /locus_tag="PLA58_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61379.1"
                     /translation="MMNKIVIRYADKRIVKGVTVDFAPNRDIFHIAPVGSSPSDKPLE
                     VRIPDLKAVFFVKDFAGNPKYEDKKNFEPGKPVLGRKIRVLFKDGEEMIGTTQGYQLN
                     RPGFFMIPADSQSNVERCYVVTKATREVKFI"
     gene            complement(31292..31750)
                     /locus_tag="PLA58_00890"
     CDS             complement(31292..31750)
                     /locus_tag="PLA58_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOR61380.1"
                     /translation="MIRSEKEIQDPGEIDAILQSAAVCRIAMCEGDRPYIVPVTFAVR
                     NSRLYFHCAKSGKKIDMLKANASVCFEVDIPGELVRGQTACVWGMKYRSVIGFGQAYF
                     IEKAGEKKKALDLLMKKYSGKESFVYADDALDKVLVVGVRIEKMSGKTAR"
     gene            31925..32734
                     /gene="murI"
                     /locus_tag="PLA58_00895"
     CDS             31925..32734
                     /gene="murI"
                     /locus_tag="PLA58_00895"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOR61381.1"
                     /translation="MNIQTHRAIGVFDSGIGGLTVVRSLMERLPFENIVYFGDTARVP
                     YGIKSVETINRYALQITEFLLKKDVKLLIVACNTMAAVAYQAIRDLSPVPVLEVIEAS
                     AKIAVDRTRSKAIGVIGTPATINSNAYARAIHLLDRDAKVFSQACPLFVPLVEEGWFD
                     HPATRLVAEEYLKPVMAERVDTLVLGCTHYPLLKPLFQDITGGQVRLIDSAEAMADIA
                     ADLIKQKNLGNTGRRPPEYLFCVSDVPYRFQTIGERFLGRTLPRVEMVRLQ"
     gene            32731..34203
                     /locus_tag="PLA58_00900"
     CDS             32731..34203
                     /locus_tag="PLA58_00900"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOR61382.1"
                     /translation="MMKVLLAGYNVDYDLIRDLREKSGLTQDITPETISAAYARISRS
                     PKSVDQLRVDARAEVEKARRSNRNIVFEMGHSSVAEHAVFNIDVIGVSRLLVEEIEKF
                     RLCSYTEKSQRYVLFDRDFVVPEEIVRAGMEKDFVETVRMQNDFYHELYEWLRPWVFE
                     QNRELAENPANRSLLEGWAKEDARYAIALATRTQLGMTINARNLELMLRRLASLPLAE
                     ARLCSEKLYAATRDIAPSLIRYTRATDYDRLTRPRLRTKAEMLLAKRPQEAPSGKFAD
                     VRLVSATPGADRQAAAALLFASSGLSYGQCQSRSARLNHRELKGLFQTVFDNMQPYDA
                     ALRELENIDLQFELVVSAGCFAQLKRHRMATIIAQDYDPRLGVTVPPSVRAVGRQKKF
                     MTVMEATQRAHDRIRNKLPSAPPYLLTNAHRRRVLMKFNARELYHLSRLRADAHAQWD
                     IRNLSEKMLRQARRLMPLTLMMACGKDGFAALTQKVFPGA"
     gene            34228..35604
                     /locus_tag="PLA58_00905"
     CDS             34228..35604
                     /locus_tag="PLA58_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR61383.1"
                     /translation="MTYQRKWPIVLLATTISSFTTPFMGSSVNVALPAIGRDFSMDAL
                     SLSWVASSFLLAAAIMLVPLGRLADLFGRRIFFLSGSLIFSLSSLLSIWPETESLFIA
                     LRVVQGIGAAMVFSTGTALLVSAYPAGERGKMLGINIAAVYTGLTAGPFIGGLLTQHL
                     GWQAIFIVNAALGLAAAVLAVRLEKEAPPAAAGESFDLGGSFLYATALFALMYGFSRL
                     PSLSGAVLIAGGGVCFMVFIRGQLKKRSGLINLHLFMGNRVFAFSNLAALIHYCATFA
                     ITFLLSLYLQHVKGLSPSQAGFVLVIEPLLQAVFSPLAGRLSDRYEPRIISSIGMSIT
                     VVGLAGLIFIASDTPLYGIVCCLALLGIGFALFSSPNVNAVMSSVEDRFYGLASATQA
                     TMRMVGQMLSMGIAMLVFAWIIGNHGIDHENGALLMVSAKSIFAILAAICTAGVFASL
                     ARGRIHKA"
     gene            35671..36435
                     /locus_tag="PLA58_00910"
     CDS             35671..36435
                     /locus_tag="PLA58_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOR61384.1"
                     /translation="MKAQGISFAIGLAAGCFGGLIGLGGGIIMVPLLVELLHLDQCRA
                     HGTSLVVLIFTGISGGISYGLHGQVDWKAAVLLAVPAMLLAAAGACLADRLPDWKLKK
                     IFGAFLIFCSALLLLKPVLADWIGVLPDAVSDIALVATGAATGFLSGLMGIGGGTIMI
                     PAMILLGGFTQHIAQGTALLVMVPSGFVGAFTHWKLDNVAVELLFSMVPGIVLGAFAG
                     AGIAQFIPEAPLRWMFVLVAVYMGWRYVKVVSPDTC"
     gene            complement(36577..37869)
                     /locus_tag="PLA58_00915"
     CDS             complement(36577..37869)
                     /locus_tag="PLA58_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR61385.1"
                     /translation="MPPSDRKPKGAKRTPWLWVPSIYIAEGIPYMIVMTVSVVMYKNL
                     GLSNTDIALYTSWLYLPWVIKPLWSPFVDLLRTKRFWIIATQLAIGGSLACVALTLPA
                     SDFVRYTLAMFWIMAFSSATHDIAADGFYMLGLSTPEQAAFVGVRTVFYRVATIASKG
                     GLVILAGTLSQHGYPVASAWSVTFVVVGAFFLALCLYHFLILPRPEADHSAPLNRGRG
                     MVAEYFRIITLFFRQKDILIIVCFFLFYRFAEAQLVKMVAPFLLDAREKGGLGLSTAE
                     VGWIYGTVGVVALMLGGLLGGYVIYRNGLKFWLWPMVIVMHLPDLVFVYLSHAQPENL
                     WIIGSAVAFEQFGYGFGFTAYTMFMIMVSQGEYKTVFYAIGTGIMALGMMFPAMSSGW
                     IQEKLGYIHFYYWILLTTIPGFMVTALVKIDPEYGKKA"
     gene            complement(37883..38317)
                     /locus_tag="PLA58_00920"
     CDS             complement(37883..38317)
                     /locus_tag="PLA58_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61386.1"
                     /translation="MNRKTRLLKRYGGLLAAILLIMVLAACGASQKYVLRSEQDFQRA
                     FNDVYFQGEAQMEAPVKYGRIDLLSSEYAIEVDSPENFHEAIGQALHYAKETGKKPAV
                     AFYILEDEPGHREKLKYAVWLCNYYKIKTWFINEELKKARKK"
     gene            complement(38314..39636)
                     /locus_tag="PLA58_00925"
     CDS             complement(38314..39636)
                     /locus_tag="PLA58_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOR61387.1"
                     /translation="MIDREPDIQAALLQHTSEARMILEGVFVLPDGHGINGEHTARGA
                     DGLIVLTDSDGREIFQGKNLLLSPAMPAESFFTVPDVTIGIDFHWQRVQKQSFRGSLF
                     LSGQSPTAFHLINIVPLEEYLASVISSEMSAGAPLEFLKAQAVTSRSWLVAMLDRKKT
                     HRLPPPNRQDEISVWQDVNDHEGFDVCADDHCQRYQGISRIASENVARAIGATRGVFL
                     TSGDQICDARYYKCCGGRTDVYATAWEDASFPYLSGVADADRPGAPIATEQDAAGWLR
                     SRPDAFCNTADQNLLQRILPSFDLETLNFYRWRVAYSRQELEQILRARSGIDFGTLQN
                     LEPLARGPSGRIFRLKVVGSRRTVVVGKELEIRRWLSPSHLLSSAFVVSVERDPDGTA
                     QNFLLEGGGWGHGVGLCQIGAAVMAVRGFRAEEILAHYFAGAQLRKLY"
     gene            39867..40787
                     /locus_tag="PLA58_00930"
     CDS             39867..40787
                     /locus_tag="PLA58_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61388.1"
                     /translation="MAHIWPIGGGKGGSGKSFITSSLGRLSAGLGRRTLVIDLDLGAA
                     NLHTLVGVPYPSKSFSDFLQKKVTRLDDTVVETPFENLFLISGAQDPFDIANLPYEQK
                     IKALRAIMRLDYACILLDLGAGTSFNTLDFFLLSQNGIFITTPEPTSIENVYRLIRSV
                     YFRRIRYCFSVSQFKALEEEAIEQFGEGAFNKPECMMKVIERDHPESYRELQNDFNAF
                     RFRLVINQLHKRDNAVLGLHICRIIEKHMGLGIEFTGNVSYDDHVHDAICQQVSFLDR
                     YPYTKTAGELRAVGRQIFQPAGRQLMLQYV"
     gene            40828..41508
                     /locus_tag="PLA58_00935"
     CDS             40828..41508
                     /locus_tag="PLA58_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR61389.1"
                     /translation="MRKSFAELNYYEMLDIRPDAAAFEIRQAYDAALQIYQPGSLVSH
                     SFFTDAERREILSLVEKAYRTLIQDQLRKAYDDELVRRGELDAVRETAPAVKKPVGIF
                     SISRGRGRQPAGPNPESLKNRILQSARIRELTGRPALSGEDLKQIREELDVTLEEISQ
                     ATKIRQDHLINIEEDCFARLPAAVFLKGFVKSYLKYLCLKPAEELAARYMETFARLSQ
                     SQRAEENK"
     gene            41505..42185
                     /locus_tag="PLA58_00940"
     CDS             41505..42185
                     /locus_tag="PLA58_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR61390.1"
                     /translation="MKGATMEFDTITTAEGDLGIRFLGHSSLIFFWAGKTIHVDPVSG
                     EADYTKLPKAELILITHEHYDHLDLKAVDIIRTAETKIVGNGEVGQQIPGTIVLKNGD
                     TRMVDGLKIEAVPAYNLQHMSTPGNPFHPKGIGNGYIVTFGDLRLYIAGDTENIPEMR
                     RLRNIDIAFLPMNLPYTMTPEMVADAARMFRPKILYPYHQGETDTAKLLALMKDEKDI
                     DVRIRTMK"
     gene            42393..43223
                     /gene="dapF"
                     /locus_tag="PLA58_00945"
     CDS             42393..43223
                     /gene="dapF"
                     /locus_tag="PLA58_00945"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOR61391.1"
                     /translation="MNKNGIAFWKASGSGNDFIIIDNRDLSLNVGELPAFARSVCRRK
                     VSVGADGLLLVEPSSAADFRWRFFNADGSAAAMCGNAARCVARFAFENGIAGQTLSFE
                     TQAGIIRAEVAGDVAKVRLTDPSSLKQDIRVHLSGGECGLDCIDTGVPHAVCFVASAE
                     ACDVTATGREIRRHEVFQPEGANANFALVINRHKMRVRTYERGVEDETLACGTGVVAS
                     VLAAAGRRFVDSPVDVIVQSGETLRVYFTRRDGAFSEIYLEGKVKIVYQGTLFEDAYR
                     "
     gene            43241..44419
                     /locus_tag="PLA58_00950"
     CDS             43241..44419
                     /locus_tag="PLA58_00950"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOR61392.1"
                     /translation="MFIVQTAERLKKIPPYLFMELRKKINQAKADGVDVISLAIGDPV
                     EATPGSIIDELCRTARDPLNHRYPTDEEKGMLALRREIARWYARRYGVELNPEKEILG
                     LIGSKEGCHHFVLARVNPGDIVLMTDPGYPAYRASILMGEGVPCNVPILPQNDYLPAL
                     EDIPSETAKKASAMFLNYPNNPTGACATQEFLNKLVAFAREYDIAVCYDNPYGEIVFA
                     GQERISFLQAPGAKDVGVELNSFSKPFNMCGWRIGMACGNPDLIAAISKVKENTDSGI
                     FNAVQFAAIQALTGEAGFIEEMLSIYGRRRELVLKTLQKIGIDFQPPRGTFYLWVPAP
                     KGMTSLEFTNRLFDKTAVVVAAGTAYGQYGEGFIRISLTVPDDRLQEAMERIEKEFAP
                     "
     gene            44471..45502
                     /locus_tag="PLA58_00955"
     CDS             44471..45502
                     /locus_tag="PLA58_00955"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOR61393.1"
                     /translation="MEMGILGLPQSGKSTLFEIMTGVKVSPSHTETVVRGQASVPDAR
                     FDRLVEIYKPAKVSPARVPFIDVHAVGEHPWEAIRQNLSGADGILHVVDGFSLPDISI
                     ALDAYRKLEDELVLSDLLIIEKKLERLARMSKKAIPAQEAAQNELLPRLKDCLEEGRP
                     LRRAGLTAQEVFTLKSFSFWTIKPELVVVNVAEDNPAFADEFAEAAHLACPVMGICCK
                     VEAELAGLDAADQKEFLASMGLAEAAFGRIIRAAFSLLGRISFFTVGEDEVKAWVIPQ
                     NTKAPKAAGAIHNDFERGFIKAEVMSFDDFMAHGGSHARVKSAGRLRLEGKEYVVADG
                     DIITFRFNV"
     gene            complement(45557..46039)
                     /locus_tag="PLA58_00960"
     CDS             complement(45557..46039)
                     /locus_tag="PLA58_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61394.1"
                     /translation="MRKIFWIGMILMLWAAPSAAVAENALTIYFKDGRHMTIPYRDIT
                     RIEFQSSDQAGHPGYSREISVSGSWKTDNGDIQLWQTGNQVSGAYLPQEHGEIIGTLS
                     GNTMSGFWIEDMSGTKCPYAKNGRYYWGKLRFVFDGSRFSGQWGYCDAEPNQSWNGKR
                     "
     gene            complement(46121..46423)
                     /locus_tag="PLA58_00965"
     CDS             complement(46121..46423)
                     /locus_tag="PLA58_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61395.1"
                     /translation="MILTGTRWQATYLAFETARETFPGDLCEKDDEEGAIKVHRNSFF
                     HGNTLITIRFQRINDNNWFVNVSSEGTGLNQPLGNRSASETELYLKALEDAAARTR"
     gene            complement(47098..47192)
                     /locus_tag="PLA58_00970"
     tRNA            complement(47098..47192)
                     /locus_tag="PLA58_00970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47156..47158),aa:Ser,seq:gct)
     gene            complement(47259..47344)
                     /locus_tag="PLA58_00975"
     tRNA            complement(47259..47344)
                     /locus_tag="PLA58_00975"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47308..47310),aa:Ser,seq:tga)
     gene            complement(47376..48650)
                     /gene="serS"
                     /locus_tag="PLA58_00980"
     CDS             complement(47376..48650)
                     /gene="serS"
                     /locus_tag="PLA58_00980"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOR61396.1"
                     /translation="MLDIKYLRQNLEAVRRKMSERGQNIDFDRFVGLDAKRRDILQAV
                     ETLRNERNSVSKQVGELKKKKEDASELIAKMGDVSAKIKEYDETLRVTEEELNAFVMI
                     VPNIQHESVPQGGGSEDNTVVRTWGEKPSFCFAPKQHFELGENLNILDFARGAKITGA
                     RFTLYRGAGAQLERAITNFMLDLHTANHGYTEVFTPFMVNAESMTGTGQLPKFKEDLF
                     KIEGMDYYLIPTAEVPVTNVYRDEILEEEKLPVYLVAYSACFRAEAGSYGKDTRGLIR
                     QHQFNKVEMVKFSRPETSYDELEKLTANAEEVLKRLGIPFRTVCLCTADLGFSSAKTY
                     DVEAWMPGQDTYREISSCSNFEDFQARRAAIRFRRKDSGKVEFVHTLNGSGLAVGRTV
                     VAVLENYQQADGSVVIPEALRPYMRGLERITP"
     gene            complement(48744..50336)
                     /gene="murJ"
                     /locus_tag="PLA58_00985"
     CDS             complement(48744..50336)
                     /gene="murJ"
                     /locus_tag="PLA58_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOR61397.1"
                     /translation="MDDPTEKSENAKVAKAAGIVGAATMVSRVLGVIRDMVIAAFFGA
                     SWTTDAFWVAFRIPNMLRRLLGEGSLTVSFVPVFTEYLQKKTKPEALELADNAFTILS
                     VILAAVSALGIALSPVIVGLIAPGFMANPEQYELAVFLNRLMFPYIFFIALVALCMGI
                     LNSFRHFTSPALSPVMLNIAMITAALVLHDAFDQPITALAAGVLAGGVLQLAMQRPAL
                     KKFGVSFHFRFNLNHPGIRQIGALMLPAIVGAGVGTINVFVGTVLASLLPGGSVTYLF
                     YADRIMEFPLGIFAIAIGTAALPSFSAHAAAGKTEELKSGISFSLRLMLFLTIPSMVA
                     LMALHLPIISVLFQRGAFDAQAAVYTGQALFCYALGLWAYSVLRVFVSAFYSLKDSKW
                     PLKAGIITLVVNVVFSLTLMYPLRHNGIALAGSISAAVNVGVLAFVLRNKIGPYLDRA
                     FYTSVFKIAASSAAMLAAIGLVEYGMPWNTSSGFRDRLIYLSFAIGAGACAYFTCAHL
                     LKSPEMRAFLKIAQKRLTSSGH"
     gene            complement(50370..50687)
                     /locus_tag="PLA58_00990"
     CDS             complement(50370..50687)
                     /locus_tag="PLA58_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HOR61398.1"
                     /translation="MTDTLLIRETKSGLSFDISVNPHASRAQIAGISEGLLKVKVTAP
                     PVEGAANDACIELLAKKLGLKKSRMKISAGARGRKKTILINDVSRMELEQKLRQLDLH
                     SES"
     gene            complement(50702..51004)
                     /locus_tag="PLA58_00995"
     CDS             complement(50702..51004)
                     /locus_tag="PLA58_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOR61399.1"
                     /translation="MLSIGMSNFIVALAKVIDIVLTVYLWIIIARALVSWVNPDPYNK
                     IVVFLYRVTEPVLGPIRRMLPRHSLPIDFSPLIVLLIIIFLQYFLVQTMIQAARGF"
     gene            complement(51179..51970)
                     /locus_tag="PLA58_01000"
     CDS             complement(51179..51970)
                     /locus_tag="PLA58_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR61400.1"
                     /translation="MLKYFKVRENRIACWVNPGDFGAHAQSLVFLHGSGGNSGAWVEQ
                     YSVLHKSFNIAAVDLPGHGQSEGSGERDISAYVLRLKEILDVLKLARPVLVGHSLGAA
                     IALGFAAQYPQEVSGVVAAGGGVTMPVNPDILDGFAKQPDLILDLMCKFSIAKENRPK
                     LFGPLRESLGQASVDVVAGDMLACGKFDLTEELQNIRVPVLALCGMEDKMTPPASSEK
                     IAGGITGAKCVLIEGAGHMAMLEKPEAFNDALQNFVAALPQTIEK"
     gene            52091..52906
                     /gene="proC"
                     /locus_tag="PLA58_01005"
     CDS             52091..52906
                     /gene="proC"
                     /locus_tag="PLA58_01005"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOR61401.1"
                     /translation="MFTGKKVAIIGGGKMGGILAQGMISRKLLSPQNITVTDIDPQRL
                     EELRSSLKLRVTGDNAEAVKGADIVVLAVKPQNFPETLKGIRKASNSSKLFISIAAGV
                     TTDSIEKLLTRKPRVLRVMPNVAALAGEGAAAVARGHFARKDDAQYALAMLGAVGLAV
                     EVEEKLMDAVTGLSGSGPAYCFVIIEALADAGVQLGLGRDLAEKLAAQTMLGAARLCL
                     QENQHPARLKNMVTSPGGTTAAGLRVLEEGKIRATLTGAVEAAAKRAKELAGS"
     gene            53043..53828
                     /locus_tag="PLA58_01010"
     CDS             53043..53828
                     /locus_tag="PLA58_01010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR61402.1"
                     /translation="MEIKGKTIIVTGAASGIGRGLCQRFAKEGAKAIVAADINEAGAK
                     AVAAEVGGEAFVCDVRKEADVAGLVKFTEDKFGGVDLFCSNAGIIAIGGYEVDNETWQ
                     RIWEINVLAHVFVARAVIPGMIRRGGGAIMITASAAGLLSQIGSLPYSVTKHAAVGMA
                     ENLAITYGGQGIQVFALCPQAVESEMTRGHEGGGVAGVDGLMKPEQLADAVMESFAAD
                     EFLILPHREVKTYMQRKAADYNRWIQGMRRLQERFFTGAPLKK"
     gene            53883..54230
                     /locus_tag="PLA58_01015"
     CDS             53883..54230
                     /locus_tag="PLA58_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOR61403.1"
                     /translation="MGGESCVSVDNLQDLEQALLVADRVIVLVHATWCPFCRKVLPVF
                     EKLARKENQHLLLVADDEERIADVYDVDIFPTLILFEKGRVVKRLDGKPGVGLSEKQI
                     ADFIKSCRPAEPG"
     gene            54310..54558
                     /locus_tag="PLA58_01020"
     CDS             54310..54558
                     /locus_tag="PLA58_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61404.1"
                     /translation="MSDVQRLLGPAFRLTTDPAGAPHKTGLLVCGCPTACAENPENSN
                     RARRWVVVAGKTVSARELTEDRLAEAVAEEIKKIIFSE"
     gene            54699..55436
                     /locus_tag="PLA58_01025"
     CDS             54699..55436
                     /locus_tag="PLA58_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR61405.1"
                     /translation="MADRVRAKTRKDRIMDAALRIFAEKSFQEATISEISKEAGVSEA
                     TIYEYFGTKEDLLFAIPEKISNDTYEESKAVLPYIKDVEGRMRAIMLSYIQLYESNPH
                     YSALVLLQLMSNKRFRQTAAHAAIRRSAHSLLDCIREGIANGTFRPDADAYLIRSMLM
                     GTIEHLFIHWHMQGMPRRDKTMMEMLDPFLEIVLSGIRVRKEQQGLTLHLNLEDARAL
                     GQILQNDPALQSPAQSADASCATKEKN"
     gene            55469..56968
                     /locus_tag="PLA58_01030"
     CDS             55469..56968
                     /locus_tag="PLA58_01030"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOR61406.1"
                     /translation="MRTKQDFINALSKMKRNIYYDGQLIDRTDELQSDCINVMGTTYD
                     EAAKPENEVLMTATSHLTGQKINRFTHIHQNTTDLHRKQDMTRMLCQKVGGCIQRCMG
                     IDGTNAIYNVSFEADKVKPGETNYHENFKKWLTRFQKEDMIAACAQTDTKGDRMLRPA
                     EQPDPSAYVRIKERTKDGIIVEGCKLHISEASVSDEVLVIPTRALRKEDKDYAVAFAV
                     PADYDGMKQVVTIHNYRQREHFKRGFTPGLTDSYLIFDNCFVPWERVFLAGEHLYGGV
                     LALLFALFHRHSYSGCKPAIGDVILGTAALAAECNNVHKESHVREKLAEIIMTIELGY
                     AAGYTASDLGKPEVYIPGVGFMPYGPGSYIPNSIYCNVGRCLSGEAVWREAEILCDIA
                     GGVVATFPHEKDFVNPDVGPALLKYTKRNPKMSAEDQAQFWRYLGDSMCSATGGIANV
                     GNYHGGGSPIMEQIAITTQYDIESRKKLVKFIAGMSGGDREALAPKVKK"
     gene            57040..58542
                     /locus_tag="PLA58_01035"
     CDS             57040..58542
                     /locus_tag="PLA58_01035"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOR61407.1"
                     /translation="MRTKQDYIKGLSKMKRNIYFDGQLIDRDDELQMDCLNVIGTTYD
                     EAAKPENADLLTATSHLTGQKINRFTHIHQNTTDLHRKQDMTRMLCQKVGGCIQRCMG
                     IDGTNAIYNVSFEADKVKPGETNYHENFKKWLTRFQTEDLVGCCAQTDTKGDRMLRPA
                     DQPNPGAYVRIKERLKDGIVVEGCKLHISEASVADEVLVLPTRALRKEDADYAVGFAV
                     PGDWDGLKQVVTIHNLRNREHYKRGFVPGATDSYMIFEDCFVPWERVFLAGEYQHGGA
                     LALLFALFHRHSYSGCKPAIGDVILGTAALAAECNNVHKESHVREKLAEIIMTIELGY
                     AAGYTASDLGKPEVYIPGVGFMPYGPGSYIPNSIYCNVGRCLSGEAVWREAEILCDIA
                     GGVVATFPHEKDFVNPDVGPALLKYTKRNPKMSAEDQAQFWRYLGDSMCSATGGIANV
                     GNYHGGGSPIMEQIAITTQYDIESRKKLVKFIAGMSGGDREALAPKPAKK"
     gene            58736..59542
                     /locus_tag="PLA58_01040"
     CDS             58736..59542
                     /locus_tag="PLA58_01040"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOR61408.1"
                     /translation="MLAMFDLSRFSLEGKVAIVTGGSRGIGQGIAYGFAKAGAKVAIT
                     SRKAQDLEATACEMTNIGCEVLVVPAHLGKMEEIQRVVDTVMQKFGRIDILVNNAGAS
                     PAMGSVLDSDERLWDTIMNLNLKAVYFMSQACAKIMKKQGGGKIINVASIDGVNPEPF
                     VSVYSISKAGVRQITRAFAMELARDNIMVNAILPGPISTKMMNSHWQHLPPEEAARQK
                     EELCKHLPTCRIGEPDEIAGAAIYLASDASSYTTGAEIIIDGGLLLGPHL"
     gene            59644..61602
                     /locus_tag="PLA58_01045"
     CDS             59644..61602
                     /locus_tag="PLA58_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR61409.1"
                     /translation="MKTLEHLLSPIKIRSLEIPNRLVMSPMGTALGNPDNTVSEANLA
                     YIRRRAEGGAGLIITEITEVHPLGSASPRCIGVWDDKFIPGLSKLADVVHAQGSKIAM
                     QLHHTGRENYLLQKKNKAVGPSAIPSYVFGFLGTPREMTLAEVEETIAAFGSAAVRAR
                     KAGFDAVELHGAHGYLLMQFLSALSNQRKDRYGGDFRGRARFMIECLREVRRQVGTDF
                     PVSIRISGEECIKNGYTISDMETILPDLVNAGADIINVSFGTHGSPAVNIDTPNPSAP
                     VEYPPGFKSHLARRVKEAAGVPVISVGRYTDPYFMDEVIARGDADMIAVARQHLADPD
                     FLKNALAGHPEDTLECLACNQGCIERLSLEALPIRCAINPQTGQELMYPAGPAAVPRK
                     VWVVGGGPAGLTAAYEAARLGHQVTLFEQESQTGGNVRYAAKAPHKEVYGRYIQTLTA
                     KCKKKGVEIKTGAEVTEAMIEAGKPDAVILAIGAGKAACPAEGIDASVVCDAWQILDG
                     TVAPKEPVVVIGGGLVGMETADFLLEKGVRNITIVEMLPASPVLALAAHGYMLHKRLR
                     AAGIALMLGTRVKKIEENAVIVEVKGEEKRLEPVSQVIVAIGVTPRTALKDMLAKKSI
                     RHFVVGDASAPRRIIEATTEGAKAAWEV"
     gene            61722..62579
                     /locus_tag="PLA58_01050"
     CDS             61722..62579
                     /locus_tag="PLA58_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR61410.1"
                     /translation="MDLRERYIETNGIKLHVMESGPADGPMVLFLHGFPEFWYAWRKQ
                     IGYFADRGYLVVAPDQRGYNVSDKPAGVAAYNIDELAKDIIGLVDAYGRKQILLVGHD
                     WGASVSWWVALKYPDRVRKLAILNVPHPKVMARNLFTNQRQMEKSWYIFYFQLPGAVE
                     KFGSAHNYAWPVHLLATTSRPGAFTPEELEEYRKAFAQPGAFSAMVNWYRAMIQTQPE
                     PLQSFDVVMPMILMWGEDDVAMLAEMADQSMAYCKQGRLIKFPGVSHWIQHEEADRVN
                     ALIEELFIA"
     gene            62653..63255
                     /locus_tag="PLA58_01055"
     CDS             62653..63255
                     /locus_tag="PLA58_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61411.1"
                     /translation="MKKGIVILGAGLILLLMSGAATAQVKSPAMIRTAYSAMDANGDG
                     VVTVTEHAAFWQGRFKDMDADRDGKLTAAEFNAAEKRFFGDMDTDDDGALLAKEYIAY
                     WCGPKALEPAKSKAKTKTKSKKKLDADKNGKIGDDECVAFWVAEIFDVDANQDGKVTS
                     EEYMAAMASAFKEMDKDRNGFISIEEHAYFIAKKAGAAKK"
     gene            63423..64634
                     /locus_tag="PLA58_01060"
     CDS             63423..64634
                     /locus_tag="PLA58_01060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOR61412.1"
                     /translation="MSREVVFVDGMRTAFGTLGKTLRNILGEDLAGMTLKGLVERSKI
                     VEKGGVVDGVFMGSAIGPNTAINGARWPTLASGLPESTVASYIEMQCGSAIDSINHAA
                     WKILAGQSDIMIAGGYESYSQAPCRFSMSQEPFKMIPPLPIAPALSPTRDPATMNMGL
                     TAEALQKMYNISREAQDEFSYRSQMRMKAAQESGFLAQDIMPVMVPQGKKKPPLEFSV
                     DEFPRPDTTMEGLAKLPPAFIKDGTVTAGNASGRNDGAAFVLMMTKEKALQLGYKPWA
                     KWLSGGDYGVDPKIMGIAPAYAVPMALKRAGIKMSQLDVIECNEAFAVQNLAVAAELE
                     KQTGEKVDWNKWNPNGGAISIGHPNGASGARICIFTMRELMKKGGKYGCFSSCCGGGL
                     GVACVVENLTF"
BASE COUNT        14070 a        18019 c        18413 g        14012 t
ORIGIN      
        1 atggacatga acccccaaaa gggaatcgac gaagatttcc gatataagga gggggagcat
       61 gtccacgagt tttctgtacc acgttttcgg tcttgtaggc tataactatg ttaagacaca
      121 gtaccaaggc aatgcgatta tattcactat ttctcacaaa agagaaaaac tgcaatgtcc
      181 ggcctgtaaa agcaggaaag tcattttgcg ggggacaatc aagcggcgct tcaaagcgtt
      241 tcgctttggg tttaaaatca tctatttgga gttggaggtt cagcgggttg gctgtttgga
      301 gtgtgacagc gtccgtcaag tttcccttgg ctttgccgat ccgcggtttt cttacaccta
      361 tgcgtttgaa cgctatgctc tggagctgtc gaaacatatg acgattcaag atgttgccaa
      421 gcacctgtcg gtatcctggg atgtcatcaa agacattcag aagagatatc tgacgaaacg
      481 attttccaaa ccttgtttga aacacctcaa gacaattgcc attgatgaaa ttgccgttcg
      541 taaagggcat gtctatctga ccgttgtcat ggatatggac agcggggcca tggtgtttgt
      601 agggaacggc aagggggctg acgccctcga acctttctgg aaaagattga aacgggccaa
      661 ggccgagatt gaagccgtcg ccatggacat gtctccggcg tatcttggcg ccgtcacgga
      721 gcaccttccg aaggctctcg ttgtttttga tcattttcat gtcatcaagc tcttcaatga
      781 tcaactctct gaatttcgcc gcgaccttta tcatgaagcc aaggatattc ttcagaagaa
      841 aatcctgaaa ggcacccggt ggcttctgtt gaagaatccg gaaaaccttg attccgtcaa
      901 aaaggaaaaa gatcggctgg aggaagctct ccaattgaac aaacctcttt cgtgcgccta
      961 ctacctgaaa gaagatcttc gtcaaatctg gctccaaaaa accaaggcca aagcagagcg
     1021 cgtattaacg gattggatca aaagatcagc atcttccggc atcaagatgc tgatgaagtt
     1081 tgccaaaacc cttgctgcac atcgctcagg aatcctcgct tattacgatt accgaatttc
     1141 ttcgggcccg ttagaaggaa ccaacaataa aattaaaacc atgaaacgaa tggcctatgg
     1201 cttcagagat atggaattct tcaaactgaa aatcatggcc attcatgaat caaagtacgc
     1261 tttagtcgga tgaaccacaa aaaacacttg ccaatcaata cattgaaggg agaacaaatt
     1321 taccgtgaaa taaatggggc atctgacccg ttcctttgtt cccatgcgcc gaccgcaatc
     1381 aaaacagccg ttccgttttt acagatgatc acgcgcgcag gccgctcagg aaatgctcca
     1441 gggcctccat atacagggat tgatgttcgc ctttgccgag gatggttttt tccagcttct
     1501 tgatctggat cacgccgttg aaagtgctcc agagaataaa ggcatgccgt cgggggtcca
     1561 ctttcttgaa gtgtccccgg tcgattccct cgcggatcgt gtcggccaga agagcgatgc
     1621 tggcgttgcc gtgcgcgtcg atttctttct tcagtacggg ggaaaagatg gttcccggcg
     1681 acgccagaaa atagttgatg atgtcgaagt aatttttgtt ctcttcgctg aagcgcaaat
     1741 agacctgggc gatggcgcgg attttcttct cgggcgtcgc tttcttgtcg atggccgcca
     1801 ggatggcgcg gtacagcagc tccagcccct ccacctgaag cgccgcgaaa atttcctcct
     1861 tgtccttgta ataaaaatag atggcgccga tgctgagttc ggagcgccgg gcgatctgat
     1921 tgatggaagt ggcgttcaat cccttttcca gaagaagttc ccgggccgta tccagaatat
     1981 gatttttgcg ccgctctttt tctctttcgc gtctttcttt ggctcccata tctttcatcc
     2041 ttgctctttt ttcatctttg ccgcctgggt gtcccctttg ggtctcatat caaaccaggg
     2101 ttgctgcttt ttaaaagggc accaggccgc ccggcaatat cgaatcgggt cgtcggtcgg
     2161 tatttttatt ttcggcagca ggccttcccg gtggtttttc acccaggctt cggcttcctc
     2221 ttttgaatcg acttccccgg cgctgtattc ggtccttccc cggcaatgca caaagccgta
     2281 aatcagttga taggattcgg acatgcctcc tccgtctttt aaaattcctc gccgattttc
     2341 aacagcactt tgacggccag gccggtgtcg attctttccc gatagacgtc gggcaggcgc
     2401 gaaagcggct cgtagtcgga gatcagcggc cgggcgtcca gtttgccctg ggccatccag
     2461 ttcagacact gaatgttttc cgcgtgcgtg ttggagttgg aagccgtcag acggatttcc
     2521 ttgaggttga ccaggaaagg cgcctccagg ctgaccgggg aaaagatcat gctcacgatg
     2581 cagatttctc cggcgtcttc cgccagaagc acggcgcggt tgatgttggc cttgacgccg
     2641 gagcactcga atattttccg gtagcccccg ggcgcagccg ccatcgcttt ctgatccgcg
     2701 tcttttgcca gcggatcgaa gacctgatcg gctccgtaca aaacggcgat gtcgcgcttg
     2761 gacgcgaccg gttcgaaaag aacgaccggg ctgtagccga aaattttgag cagccggacg
     2821 gcgcagaggc cgatggggcc gcctcccatg accagcgccg atcctttccg gtcgctggtg
     2881 cagttgatgc cgtgcagcgc ggcggcaaag gtttccgcga gcgccgcgtt tcgggaatcg
     2941 acgccgtccg ggatgggaat cagcatctgc ggatagactt tgacatactc cgcgaagccg
     3001 cccggcaaat cgccgattcc ggcggtccgc cggtgaaccc ggcagaggtg cggccggccg
     3061 gaaaggcagt tggcgcattt tccgcagtag gtgggacgga tgcagactct ggttcccaca
     3121 ggggggccgt cggcggccgc ccctttttcc acgatgacgg cgctgatttc atgtcccagg
     3181 atatagccgt cggccagatg accgccggcg accagggaat gatcggagcc gcaaaatccg
     3241 gtgttggccg ttttgaccag cacctcatct tcagcaaccg tataatcggg gacctcttcg
     3301 tagacgaggc ccctgtcctt gcgaaagaca accgctttca taattccctt tttccttcgt
     3361 agtagagaac ggcggtgtga cctcgtgcca cctcgtgtga ccttcaggtc atcaggtggt
     3421 caggttatct cgaccgttct ctatattctc taattctcac attccagggt aatcttttcc
     3481 actttatcca ggtccttctt gtagaaaaaa gagccggcaa aaaacaacag ggacgaaatc
     3541 accagaaaga caggcacgat cgacatggct gcctcgatgc catgaagatc ggagatcttc
     3601 ccgatcgcaa tgggcgccag tgatgcccca aaaagattct gaacaatcac gcaaaccgaa
     3661 tacgagatcg cccgaaggcc gggatggata acctcctggg tggcgataat cgcagccggg
     3721 gcaaatgcgg aggccagaac gcccatgccc agaagcgtaa aatactgcgt ttttccctcc
     3781 aggaaggaaa aagcagtaaa gataacgagg gcattgagga tggacgtaat cgcgggaaac
     3841 aaaagccgcg cattcacccg cttcttaaac cacatatccg ccagccaccc tcccagaggc
     3901 aacccgataa tggccagaat catgagcatg cttaccttca tgcccgcttc gctcatgggg
     3961 atgcccgtcg tccggtgaaa ataggtggga agccagacca tgatggcatt gttggcgaag
     4021 atgcagccga taaacccgag attggtaaaa atcagggaag gggtgcggag aaactcccag
     4081 acaatatcca tcttgcccat tttaatttta ccgggaccgg ttgtgcaggc ggcatcggtc
     4141 ttcgccaggt ccacggtctt gtagtccttg acgaagaaaa aaaggatcgc cacgatcgcc
     4201 ccgggaatgg ccaccacgcc gaaagcgtga cgccatcccc agtgcgcggc aatgaatcct
     4261 cccagcccga tgccgacggc tgttccgaga gggatggaca tattccagag gcccatcatc
     4321 cgcgaccgtt tttcctcggg gaacaagccc gaaagcatcg ccgtgccggc cggggcgtat
     4381 ccggcctcgc cgattccgat ggcggcccgg gtcatgagca gttggggaaa ggtcttggtg
     4441 aaggcgcagg ccaacgtggc catgctccat aaaaaggcca tgatgccgat ggtctttttc
     4501 cggctccagc gatccacaag aatcgaaacc ggaagcacaa aagcgacaat ggaccagtaa
     4561 accatcgaca cgagcaatcc ggattgggta tcggtcagcc cccattcctt ctgaatgaag
     4621 ggaaaaaggg acgtcacgat cattcgatcc acgtaatcga agaaatacag cagaaagagc
     4681 agtgcgaaga tgtagtaggt gcttcgggtg gacagcttgt attccttggg ggcagtgacg
     4741 ttattcatgg ggcctccttc ttctgtggat tcatggatgt gtatcttgcc tctcttcaaa
     4801 gtcggcgtcc tgccggcaac tcattcgcat ttgtatccgc cgagattggc cagggcaacg
     4861 ctgccggggc tgatcacgga aggatctacc ttgacattga tgcaggcggt tttcccggag
     4921 gcaaaagccg cttccagcgc gggcttgatg tcttcgggtt tttccaccag cgcgccgaag
     4981 ccgcccagga cttccaccat cttgtggtaa tccacccagc ccagctccgt gccgttggga
     5041 atattgtgtc ccagccggag ggtctggctg tgcgcgatca tcccccagga ctgatcgttg
     5101 ccgatcacga cgacgatggg cagcttcttg cggattgccg tgtgaaattc catgaagttg
     5161 aagccgaccg atccgtcgcc catgatgagg agcacgcgct tatccgggtt tttgagcttg
     5221 gccgcgttgg cgtagggaat gccgacggcc agacagccgt aaaggccgta gtccagatac
     5281 gtgccgccct ttttgacggt tctcgtcatg cccatccagg tggacgtatc cccgccgtcc
     5341 gcgacgacaa tgtcatcctc gcgattcata aattcgttga cctctttggc caggcgcatg
     5401 ggatgcatgg gcagggtctc gcattcccac atcgttttgg cgtctttttt gccggcttca
     5461 tgggctgccc ggacggtatc cacccagggc tggaactgtt ttttcagcgt gtcggcgatt
     5521 ttctgatcgt cgatgacggc gttgagctcc ttcagaaacg ccttgatatc gctgacgatg
     5581 ccgagatcaa tcgtgcggtt gcggccgatt tcgtcggcgg cgatatcgac ctggataaac
     5641 ttggccgtcg atgggaaaat ttcgccgaag atgtagaaga gactcaaccg gccgcccagg
     5701 aagagcacca gatcggtggt catgagcgaa atcatggccg aacccggccg gatggccagg
     5761 gagccttcaa agcaaagggg atgcgtatcc ggcaccacgc cgcggcccgc gcccgccgtg
     5821 taaatcggaa tgccgatttt ttccacgaat tcgagaattt ctttttccgc atcggaatac
     5881 cacagaccgc tgccgccgat gacgaccgga cttttggccg ctttgagcaa atcgacaacc
     5941 ttcctcgtgt tttcgcggtc aaccggtttg gaaacgaccg tcgtacgggg cttcttgacc
     6001 gcggccggat cgaccttggc attgagaatg ttgcagggga attccagata aaccggtccc
     6061 ggccgtccgc tcatcgccgc gcggaaggcc atgtccacgt attcgggaat ccgttccgga
     6121 acatagcaga cgaaggcttt cttcaccatc ggttcaatga cgggaagctg ggtcatatcc
     6181 tgaaggtcca gcttttccat ggcttcggtg cccacacagc cggcaatgag aatgaccggc
     6241 gcgttggaaa gcctggcgct ggcaatgccg cttaaagcat tggtaaatcc cggtccggcc
     6301 gtgaccatcg ccaccgcggg cttgcgggtc atccgggccg cggcttcggc cataaagacg
     6361 gccgcctgtt cgtggcgcac gtcaaaaagg gtgatgggag tgttttccag atgctggtaa
     6421 atgggtgtaa tgtgcccgcc gctcaatgaa taaatataat ccacgcccct ttcgaccagg
     6481 gcgtccgcaa cgagcttgcc gccgataacg ttatccatnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnncg cccctttcga ccagggcgtc cgcaacgagc ttgccgccga
     6661 taacgttatc cataaaaaat tcctccttaa aaacaggttg ttgctgttga tgtgataact
     6721 gtagggaacg gtctcgtgac ctcgcgtgcc ctttagggta ttaggttatt caaaccgttc
     6781 cctacggatt cgtttcctac agttccatgc actggcctcc atcaagattg acggcctggc
     6841 cggtaatgta gcgggaggcg tcggacgcca ggaatgcgac cagatcgccc gcgtcgctcg
     6901 gcagaccgat gtatcccagc ggaatggtct tgcacatttc cttttcccgc tcttcgatcg
     6961 ttgtgccgaa aaaggatgcc tccaggccga atcgccattt ttccagatcc gtggcaattt
     7021 gtcccggaca gatggcgttg acccgaatcc cgcttccgcc cagttctttg gccatgactt
     7081 tggtcagcat gatgacgccc gccttggcta cggcgtaagc gctgttgaaa atgggaggca
     7141 ccttgccggc ccgcgaggcc gtgttgatga tcgaggccgg tttgcccgcc atcaggggaa
     7201 aaacggcccg tgacatgcgg aagacggaaa agagattcac gtcgatcgtt ttcatccagg
     7261 ccgcttcgtc gtaaccgagc accgtgttgg ggacgccgaa ggacgctccc gcgttgttgc
     7321 agagaatgtc gatgtgatcg aagttttttt tgatggtctc caccatgacg ccgatggccg
     7381 cattgtccgc cacatcgacg gcgacggcca gtgtgcgaat cgaataagtc tttgccagtt
     7441 cagcggcgat ggcttccatt tcttcgctga cgaccggctg tgggggccct tccggcgcgg
     7501 gcggcgcgcc caggtcggca atgatcacat tgcagccaga ggaggccagc ttttcggcga
     7561 tggcgtagcc gatgccggtt ttcttgccgg cgccggtgac cagggccgtc ttgcctttca
     7621 agtcattata aatcatcggt tcctccttca tcaacccagt ggcggtaatc cagatgggcg
     7681 tacttgggca ggaatcgcgt cggcagcgcc agcgcatcct tgcggaaagg cggcgcgagc
     7741 tgcttgccgt ccaccaggaa ttcgagcacg gtgggcttgc ggctcttcag gcaggtttgg
     7801 aaagcttccc ggatatcctg cggacggccg acctgcattg cgttggcgcc catcgccctg
     7861 gccaccggca caaagctttc cgggttggga atatcgacgc cgacaaagcg gttgttgtag
     7921 aagtccacct ggtttttggc ttccgcgccc caccacatgt tgcggaaaac gcaggcgatc
     7981 acgggcagat gatgctctac ggcggtgctg acttcgtgaa ggctcatgcc ccacgcgccg
     8041 tcgccgatga tggcgatcac cggcgctttc gggcgggcaa tctgcgcgcc cagcgccgcg
     8101 ggataggcaa aacccgtgtt gccgaaggtc agcgccgcga tatgccggcg aggatggttg
     8161 aacttcaggt agctgttggc cgtcgaggag acgttgccga tatccgtcgt cacgatggcg
     8221 tcgtccggca gcgcccgcga catttccagc agaacgcggc gcgggttgat cggattgccg
     8281 tccaccatcg ccagatcgac aatttccttt tcccatttct tgttttcccg ggctacggcc
     8341 gccaggcgct tctcatccgg tttgcgtttg ccgtccagcg ccttcagtcg tttcaggatt
     8401 tccgcggaag cttccttggc gtcgccgatg atgccgacct cgatgggatg cgtccgggcg
     8461 atatgcttgg gattgatgtc cacctggatg atcttcgcat tttccgggaa gtaattgatg
     8521 tcgtactgcg gcagcgtgcc gaagacggaa agccgcgtgc cgatggccag catgacgtcg
     8581 gctttggcga gcgtcttcat ggccgccttg gaacccatgt agccgatggg cccgacggac
     8641 aggggatggt ttccgtaaaa cgcgtcattg tgcagatacg tcaccgccac cggcgccgtc
     8701 aggtgttcgg caatggcttt gacgatctcc agcgcatcgg catccaccac gcctcggccg
     8761 gagacaatga caggattctt cgccctggac agaagctcgg cggcccggtc cagttgctcg
     8821 atggaaccgc agccgcgcgc gtccacacga tactgcgaag gctttagaat tttttcctcc
     8881 agctcaccgt agaaataatc gcgggggata tcatacagga cggcgccccg gtcggcgtag
     8941 gcaatgcgga aagccgttct caggcagtcg gccgctcttt tcggatgggg cacgcgaacg
     9001 acggctttgg tgatcgcgcg aaatacggac atctggtcgc attcctggaa gccgtcccag
     9061 ccgatggtcg gtgttcccgc tgaaggcgag ataatcacca ggggggtgtg gcccatattg
     9121 gccgccgcca ccgacgtcac catgttggtg atgccggggc cgttctggcc cgtgactacg
     9181 ccgcagcgtc ccgatatgcg gcagtaggcg tcttccatat gggccgcgct ttgctcgtgg
     9241 cggacaggaa taaatttaat gccagccgtc gggaagaggt ccagcatgtc cataaaagcc
     9301 gaacctacaa tgccgcagac atgatccacg ccttcggcga gcagggtttc gacaattgct
     9361 tcactgggtg tcattttgat ttttttcatt ttcaacctcc aatacattca aacgttaagt
     9421 gtctttttga gacacgttct tttcctaaat tgaaaatcaa tattttcatg gacgaaagcc
     9481 tcaatccaga tcttctttcc agggccacaa aaatatcccg ccctccagcg tccggacgtc
     9541 ggagtagccg gccccttcca gaatccgctg agccgaaaat ccccgtaaac caagcagaca
     9601 gacgcaaatt atctttttgt ctttcgggac ttcctgaatt ctccggcgca gttcatccag
     9661 aggaatattg acaaccctgg ggctgctgat gtgcgcgcgc cggaattcca gatccgaccg
     9721 gacgtcaatc atcagaacgc tgtcgtcttc ctccagaagt tttttggcct cctgtgcggt
     9781 caccggcttc atcatgccgg atttcacatt ttgcgcgacg ttgagcgccg tgacaaatac
     9841 atccagcgcc ggcgaaaacg gaggcgcata agccatatcc aaatcatcga catccgaaat
     9901 cgttgccccc aatcttattg ctgtggcggc cacatcgatg cggcgcgcaa catcgccttt
     9961 ccccacagcc tgcaagccca aaagcttatg cgagccgttt tcaacgatca atttcagcgc
    10021 gatgatctga ctgttcggat agtagtgagc cttgtcaaat ccgcaaacgg aaatcgcaaa
    10081 tgcgtcgatc cccattcgct ttgcctgcgc ctccgaaagt ccggtggcgc cgacggcgta
    10141 atcgaacgcc ttgcagatga acgtgccctg tacgccggga aacgtcgacg gaatgccgca
    10201 gatattgtcg gcaatcaccc ggccgtgctt gttggcggtg gagcccagcg gaatgtagca
    10261 gggcttcccc gtgacgatgt gcacgttttc aaccaggtca ccgccggcat aaatagccgg
    10321 gtcgctggtc tgcatctgcg cattgacttt caacgcgccg gtctctcccc gttccagacc
    10381 ggcagcctgc gccagctccg tctcgggggc aagccccgct gcaacgatga cgagatcggc
    10441 ggaatacgaa tgatgccggg tgatgacttt ccggacttgt ccgttctctc cttcgaagga
    10501 cagaatcccg gcgtcgaact ccgtctccac catctgctcg taaaaatgtt cccggatcat
    10561 cgccgcaatc tcgaaatcaa tcatgcccgg gaagagctgg ctttcgcgct ccagcagcgc
    10621 cacctcccat ttctttgctt tcagggcttc cgcggcttcg acgccgataa atcctccgcc
    10681 gacaataacg gcgcggccgt gttcgcgatt ttggacttcc cgggcgatca cttccgtgtc
    10741 ttccatgcgg gtcaggtgat gcacgccttg aagacttgct cccggtatcg ggggcatctt
    10801 gggcgcggcg cccatcgcca gaaccaggtc gtcatagtca aaggtcaggg attttcctgt
    10861 cgccgtgttg gttgccataa tccgcctgtt ttcgcggtcg atccggcaag cctttacatg
    10921 ggtatgaacg gcgatgtctt tcagatcttt gaaatactgc gtatcgcgaa cgatgccgtc
    10981 agtggttttc agcaggtcgt ccatccggcg gacttttcct tccagatagt agggcattcc
    11041 gcaggcggca taggaaatat aggagctgcg atcaagaacg gcaacatcat aatccggacg
    11101 aagccttttg atgcgcgccg cggctttcgc gccgcaggcg gatgctccaa tgatgacgac
    11161 ccgtttcggc ttcatcgatt ctccctttca aaatgcacac tcgtaaaaat ccggtaaaac
    11221 agcgtcagca tcaaaaagcc ggcggcccag acgcccaggg tgatggcgat ttccggtccc
    11281 gtcggggaat attcagtgat cgctcccagc ggagagggaa taaagccggt gatcaccaga
    11341 cccagcccct tttcgatcca gagggagaca aacacaagaa cacaggccag cgccagccag
    11401 ctttccttcc ggcggacccc ggggaagatc aggatgacaa aagcggcaat cgataaaacg
    11461 gcaaaggtcc acatccaggc aacaaggttg taacgaccgt cgagccccgc aaagaggtac
    11521 tgaaaatgat gcatatgctc cgggatctgg ctgtaaaaca ccgtaaacag ttccacgaga
    11581 acaaagaaaa tgctcacggc cagcgcatag gcaacgattt tcgcgagcgt ctgaatggcc
    11641 ttttccccgg cgtcgaaaca tccccagcgc ttgagaatca ggcagacgag aatcaggagc
    11701 gccggaccgg atgcgaaagc ggaggccaaa aagcgggggg ccatgatcgc ggtgagccag
    11761 aagccacggc cgggcagacc ggcgtaaatg aaagcggtga cggtatgaat gctgatcgcc
    11821 cagggaatgg acaaatagat aatcggtttc acccaacggg gcggcggtat ttccttacgg
    11881 ttacaagaca ggatcaccca gcccgtaacg atgttgatga ggagatagcc ggacaggacg
    11941 atcatgtccc agaacaggat cgattttggt tgcggatgca gaatcacatt cattacacgg
    12001 gagggccgcc cgagatcgac gaagacgaaa aggatgcaca tgacgacggc ggaaaccgcc
    12061 aaaaactcgc ccagcacggt gatccggccg aaggccttgt agtcgtgcag gtagtaaggc
    12121 agcaccagca tcacggccga cgcggccacg ccgaccagaa aggtaaactg tgcaatgtag
    12181 agcccccagg aaacgtcgcg gctcatcccg gttattccca gaccgtaatc cagctgacga
    12241 aggtagaaga aaaatccgac ggtggcgaca aggatcaaac aggcgatcca gatccagtat
    12301 ccgcggtttc cttttaaagc tttctccagc ataacgtcct cataacaaat agtagatctg
    12361 cgggccggtg cccaggtgcg cgcgtctgcg caggctgaag cgcgtttcta aaagacgccg
    12421 gatgtccgac cggggatcgt ccgcgtcgcc gaaggtgagc gctttttgcc tgcacgcctc
    12481 cacacaggcg ggcagcgttc ctttggcgag ccgctcctca cagaagttgc atttttccac
    12541 gactcctttc gtgcgcgtcg gaaagtccgg attgattttg gcggtaaacg gtctcgggtc
    12601 cctgtaattc atgctccgtg ccccgtaggg acaggcggcc atgcacagcc ggcagccgat
    12661 gcagcggtgg taatccatca ttacgattcc gtcggaccgc cggaacgtgg ctttggtcgg
    12721 acacacgcgg acgcagggcg gattttcgca atggttgcag aaaacgggcg gacgcatctt
    12781 ttgcagcttt tcgcctgaat aggcgtgcga ttcgccagga aagacggatg caaaaggctc
    12841 cgtccagatc catttgacct catctttggg attgccgaat tccggaacat tgtgaatcgc
    12901 gtggcaggcg acaatacagt cggcgcagtt cgccgtgcat ttcgtcagat ccaccgccat
    12961 ggcccagcgt ttggccgcaa gcgccttttg gggagcaaga ccctgccccg gaggcatgac
    13021 gatgctgttt ttcgcccagg ccgggacaaa gatcgccggc gtcatgattg tcaaagcgga
    13081 cgctcccgcg attttcagaa agtctctgcg gttcggcgtc attttttcac ctccgcggga
    13141 acaatgtggc agtcaaagca ggagggcttt gcgcccacgt aattgtggca gcgatcgcag
    13201 aactgctctt tgttgctgtg gcagcggatg caggagccgg tgaggctctt ctcgaacgtc
    13261 cgtccgtctt tcgccgtgta tatacgcttg ccttcgcgga cggcttcgtc gcgccaggcg
    13321 ttcaaaagct tcatatggtt ggcgcgcatg aaggccttgt cctcgacgca tttcttgtcc
    13381 gccgatccgg cgatttcagg cgtctccagg ctgagagcgg gcggggccgt cttttccttg
    13441 ccgtaccaga agggaaacgt caccccgatg acgaaaatcg ccaaccccag aaggatcaag
    13501 ccccatttgt tatttttcat ttttaacatc cactatgcag ggaacggccg cgaccgttcc
    13561 ctacggattc attcactatc cgctatctac catccaccat tcacgatcca ccaattttct
    13621 cccctctcaa atccgtcgac ctttccttct cacttttcat caccagcgcg ttgccgacca
    13681 gttcatgaac acccatcacc gaaacacccg gcacccagta gtccatgagc gtcgtcagcg
    13741 tcgcccggtc gatggcgcaa atgcagccca gacggttcac gccgtgcttc ttcacgacct
    13801 cccggacggc catggcccgc ggcattccgc cgcgcatccg catctccaga ttttcatcgt
    13861 tgcccagtcc cgcgcccgct ccacagcaga aggtcttttc acggatcgtg tcttcggcca
    13921 tttcgtaaaa atggtcgcag gttttttgaa tgatgtagcg cggctcttca aaaagcccca
    13981 tggcccgggc cggattgcac gaatcgtgaa acgtcagccg gacgtggtcg ttgcgcgacg
    14041 gatcgagctt cagcttgccg tttttgatca ggtccgctgt aaattcggtg atgtgtacca
    14101 tcttcgttga ttttgcgttt tcaaaccgcg tgccggtgat gggcgatacc ggttcttcca
    14161 gaaaatcggc cggcccgttc atcgtgtcca tgtactggtg gatgacccgc cacatgtgtc
    14221 cgcattcgcc gcccagaatc catttgacgc ccagacgctt ggcctccgcg taaatcttgg
    14281 cgttcagtcg cttgatcatc tcgtgggagg tgaacagacc gaaattgccg ccttccgaag
    14341 catacgtgct gaaggtatag ttgaggccga tctcgtggaa gagcatcatg tagcccatgc
    14401 aggtgtaagt tcccggatcg gcgaagtagt cggccgacgg cgtgacgaac aggatgtcgg
    14461 cgcctttttt gttgatcggc acttccactc ggacgccggt gcgatcctca atatcgtccg
    14521 ccagaaattc cagcatgtcc ttgatgccgc ccggctgaat gcccaggtgg ttcccggtgc
    14581 ggaagcagtt ggccgcaggc tccagcgccc agttgatgtt gcagccgacc aggttgagca
    14641 gttcgcggcc gatcatggtg atttccgcct ggtcgatgcc gtagggacag aacaccgaac
    14701 agcggcggca ctccgtgcac tggtaaaagt agtaaaacca ttctttgaga acatcgacgg
    14761 tcagatcccg cgcgcccgcc agcttgccga agcagcgtcc cgacgccgta aagtagcgcc
    14821 ggtaaaccga gcggatcagc tcggcccgca ggaccggcat gtttttcgga tcgccggagc
    14881 cgatgtagaa atggcatttg tccgcgcagg cgccgcagcg cacgcagatg tccatgaaca
    14941 ggcggaaaga gcggtattta tccaggcgct ccttcatgcc gtcgaggatg atctgccgcc
    15001 agttttccgg cagtttccag tcggcgtcgc ttggctgcca ggcccgtgga ttgggcatgt
    15061 ccagagccgc aagatttttc gcggcggcgc cgtagctcca agtgccggga cggaaatcga
    15121 cgggcgtttc catccaggcc gtttgcggcg gcgcgtattt gatctgcatc agttcatccg
    15181 gttcgggaat tttcgcggtt gacatggcta ctccttctcc accggcaggc cggccgcttt
    15241 cattttatcc cggaactcgt cttcgtattc tgcgtacgtg tgaaccttta cgggggcgtt
    15301 ccagggattg acgtgtcttc ggctgcggct gttgttgatt tgattgcgcg tggggctcag
    15361 gaatatcccg gccatgtggc tgagcttgct ccaggggagc caggcgataa gaacaaagac
    15421 aaagcaaagg tgcagataaa acaggatgct cgcgtccgca ggcgcagacg gcgcaaacga
    15481 cacccagccc atggccaggg tcttcacggc gttgaggtca accgggaaga agtggcgcat
    15541 caaaagaccg gttgccgcga tggccgcaat caaaagcagc gcgaaataat cgctgaacag
    15601 ggagatataa cggacctgcg gcaggacgac tctgcgcaga aagagaaaaa ccagccccgc
    15661 caggagtgta acatcgctga tataaagcgc cggaacaccg atttccaaaa agccgtccag
    15721 cgcggaaagc cccgttgcca ggggaacgac cggctcggtg aaaaagcgca ggtgccggag
    15781 cgccgcgata agcagggaga cgtgaaacat cagcccgaaa aaccaaagcc atttggcgct
    15841 gccgtaaacc ggccggccgt ccacaatttc cgtttgcgaa tttctgaaga gcgaccgaaa
    15901 caaaaagact tcgccggcca tccggccgat cgcgcccagc ggccccgcgg ggttttccac
    15961 cggattgttg tgaatccagg gcaggctttt ttcctggccg caggtggtcg gaatgcggaa
    16021 gggaacggga gctttcgccc agcggacaac gcggtaaaca aaacctccca gaaaaaggac
    16081 gaacacgcca tagggaagga cgaccagcaa ggcggtcata tccgccgccg ctgcggccgc
    16141 agccggcagc gcgagaagca aaacagcgac aaaggctaag ccaagattac gcatcggcat
    16201 caatgccact cccactgaat aaacggttgt ttgagatcgg ccatggcatt gacgatcaac
    16261 tccaccaatc cgccgtaaaa gatgccggat ttttcccaga cgccgtaggt gttgaaaagc
    16321 tcgcggtact gccctttgca gtttgaacag ggagcgcaga cgtatttttt aacatccgct
    16381 gaaggcgcat ccgcaaaggc atccagggtt tgtttgagtt tcatccggcc tgaaaccgaa
    16441 gaccgccaat ccggaaaatt gttcgacgaa atgacggcaa aacccgaccc gccgccgcag
    16501 cagtagtttt caacgccgtg cggggtcatt tcccgaaact gcggagcgat ttttctcaaa
    16561 attctgcgct gcggttccac aatgcccatc aacctgacca tgttgcaggg atcgtgcagc
    16621 gtaacgggga agttattgcg gcccggatcc aatttaatcc tgccgctcat gacaattttt
    16681 tccatcagcg gaagagcgct ctccctggga atattcagct ctccggtcaa tacccggtcg
    16741 gcaacaacga tcatggcctt gtgggcgtgg ccgcattccc ccagcgaaat tctcttcaca
    16801 cccaggtttt tggctgcctg tgcgtgccga agcgcaacgc gggcaaactg cacgtcgtca
    16861 taccagacgc cgtaattcac cgcgtcgtaa cccacgagtt cgctcgacag cgtccagtca
    16921 atgccggccg cgtcaaacag gatcgcaaag gcttccagat tctccggcca ggccagaaac
    16981 tcgccggcat tgtgcaaaag caggatatcg gagccttttt tgtcgacggg aattttgatt
    17041 ttcttgccgg ttttttcagc aatctcttcc tcggtaaatt cgagaatatc cgccagcgcc
    17101 ggcggcgtca tccccgtgct ggaacccacc ttgatctgct ggacggagcc tttttcatga
    17161 acttccttcg gagcaattcc catctgctgg ctgaaaatct tgcggatttc ccggctgatc
    17221 agggcgttgt cgcagcccag cagacagacc tgcgcgcagc ggcggcaaag cgtgcagcgg
    17281 tacgccagtt cggccagacg aatcagtgcc tgtgcgttga gctcaatatt gctggccttg
    17341 aactttcgca ggagcggatg ttcttttttg acgtacgtgc tgatgatccg gcgcaggagc
    17401 tccggcctca tcgtcggacg gtagatgtcc tggcggccgc tggccacata agccgggcag
    17461 gcatcggaac agatctggca tttcacgcag tattccagcg aatggtacag cggctgcagg
    17521 aaggtccagt tgtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatttca
    17641 cgcagtattc cagcgaatgg tacagcggct gcaggaaggt ccagttgtct ttcgcatcaa
    17701 acagtttttc gactccggcc agaaacctgt ccacaaacgc tttttcctct tccggcatgg
    17761 acggcggcaa aatgccgatg gccacgacgc cgtcgagcgt cgcgtccatc cgtttgcgct
    17821 ggtcttccgt cagttttttc agcggtttat ccatctgatc atacggcgcc ggaagcttca
    17881 tccagtcggc cggttcgagt tcggcaacct gtccgccggg tttggtaaaa tcctgaatgc
    17941 cggtttttcg aatcatattt aatccttttt cgtctccggt ttcgtttccg gaaggcccga
    18001 cgatatggcg gcttcgaccc agctctggcc tttcagaata tgcggggcat cccaggaaag
    18061 cttctgtgcc agaaggtcgt ttaattgttt ttccagcata ctcccgcgaa agttcggttc
    18121 gtcgtcccaa cggattttga agtatgcgag cagccttgta atgtaatgtg tggagcgcgt
    18181 gaaaggcaga tagatcagga agatatcgaa caggatgatg tggaaaaccg tcgcgggatt
    18241 caggtccggc ggcgtcagtg tgatcaggcc cacccagtac atccggtact cggcaagcgt
    18301 cgggtccagc gccagccagg agtagagacc gcttgcatat acgatcagaa agaaaaaata
    18361 gttgaagtaa ttcatcggca tggcgtagga ccgcaaaccg cggtccgcca gtttgatgac
    18421 cggcatgccg atgcagccga aggtgccgcc gataaaggcg atgacggcaa aagccatcag
    18481 gatgccgtaa aagatttgcc ccaaaagctg cggtgaatcg gaggcgattg ttacgccgcc
    18541 taccatagcc gttgcggcga aaaagcaagt gatgtgaaac agaataatga agataaaccc
    18601 caggtgccac ggcagcagga aaagccagta cagcgggttg cgcttgaagt attcgcccat
    18661 gtagaaattg tctttgaggg catgccagag gctgcgtcct gtgtttttcg ggcgcggctg
    18721 tgtccaccac tccggttttt catagtaaga cccgccgtaa cggtactgtt cttcatggat
    18781 cacgggatag agatcccatc ggatatgcac cggcagctta agccatttgc gaaccttgat
    18841 cgcatacacc gctacaataa acacataagc gatgtacgtc agtacaatca acaacgttcc
    18901 catggattga atcctttgaa cctttcaaaa tctttcattt ctcaggctgt tttcagacgg
    18961 gcagaggcaa ggcgcgtaag gacgaccggg acgcggagca tatatttgat atgtgagcat
    19021 cacggcccgg aacgcaacgc agcatatgac cgtatcaaac agcctgcttt cctacacgca
    19081 gcccgtcggt ttcgacaacc ccgccagctt gcaggcgccc ttggccggtc cggtcgggaa
    19141 gagctcgtag agcttcttca tgtcaatttt cagatctttg cagattttgc ggatcatcgg
    19201 ggcgatgccg aactgctggt agtaattgcg caggtagttg atgaccatcc agtgctcctc
    19261 cgtcagtttt ccatcgatct cttccacgtg tgcgtaggct tcggcaaatt cctcgctcca
    19321 tttgtcgagt tcctgcaaaa acccgtcttc atctacttca tacgttttgc ctgctaattc
    19381 aatctgcggc atggttaaaa cctcctcatt tgatcagact gttgaaaacc gcccactgga
    19441 catttttgaa cagtcttttt ttcgttttta ttttttcagt tttctcttca aataaagcct
    19501 ataatgattt tctatttaaa cacccatatg aatcccgctc tccagggctc cgcttgtttg
    19561 ttgctttgtt ctccgatatt cttgagcttt cgcgatcagc gcattcgccc attcgggcgc
    19621 gcccagcgca tgcacatggg taaagccggc cagaatattt ttccggcaca atccttccct
    19681 ggcgccgtcg atccccgttc ccttccgaat gctgaacgcg gtgtgcattt cccgatcttc
    19741 ccttgccaga atccggcagt aatggaattc gtgaccggtt atcgttgtac ccatgggaaa
    19801 gaagggattt tcccgatcca cggtcagcgt ggtgtaaccg tggccctgcg gccgtctttc
    19861 cattccgaag gccacgggca gtgcgccgac cattggataa cagcgatggc ctatcgtcag
    19921 agattctccc aggtaaatga atccgccgca ctccgcataa atcggcagac ctctttcagc
    19981 cgattctttc acacaacggc gaaaagattc attttgcgcc agtggttcag cctgggtttc
    20041 cggaaatccg cctccgatgt acagagcgtc cacgggcgga agaaccttgt tctccagggc
    20101 gctgatttgg atcacgcggg cgcccaaatc cgtcagcgct tcgatgtttt cctgataata
    20161 aaactgaaac gccgcgtccc ggatgacgcc gatggtcgtc ggctctgcaa tccctctgcg
    20221 gattcttgcc ggcttgggaa atgcgccaat tttccacgaa ggagcgcttc gggccacagc
    20281 cagcagaccg gagacgtcaa ggtaatcgcg ggccatattg cccgccttct ccagcgcccg
    20341 ctgaacaccc gtcgattcgt cgggaggaat caatcccaga tgtctctgga cgaagggaat
    20401 atccggcaga cggggaacga ttccataaac gggaagcccc gtcgattctt caatcgatct
    20461 tcgaatgacg gaagaatggc ggttcccgtt cacgcggctt aaaataacgc cctgaatatc
    20521 cactttcgga tcaagcgcct gacagcccag gaccatggcc gccgccgtcc gggttacttt
    20581 gtcacaatcg accaccagaa tgaccggcgc ggcgagaagc ttggccagct cggccgtgct
    20641 gaactggccc gcgctgttca caccgtcata cagaccgcga tttccctcaa tgatcgaaac
    20701 gccgttcttc gcggcgtgac gcgtaaagga ttgcacaacc cgttcggacc ccatgagata
    20761 cgtgtccaga ttaaaggctg gaccggacgc tgcctgggcc agccatgcag cgtcaatata
    20821 atccggtccc tttttgaagg gggcaatctg aataccttga tggcgcaggg caaccgccag
    20881 ggccacggaa agggttgttt tccccgcgcc gccccggagg gcggcgatga cgattcgcgg
    20941 gcgacgctga ttcatgacgg accatccttt attctttcgg aaagtactcc gcgagcatga
    21001 tgtacgttga actcccactc gaccagtatt tcaacacttc ctcatcgagg agttctttca
    21061 cggcgttctg gatggcgtga cggtcctcgt tgggcagcca tttggtgacg tcgcggatgt
    21121 aaaacatctt tttgtcgccc ttcgattttt cgcgaaacag ctcggtcact ttcgttttga
    21181 tatcgtcgct catgtcttac ctccatttga actgtgcgga tgtccggaag gtttccgtgg
    21241 ccagatcaaa gtcgtcgatg tgctgatccg taaacgggat gcccgtgagt gagaagaatc
    21301 gctcccagcc cacgcgctcg atccattcgc cgacgcgttc gtgctttttc gcgtgactcg
    21361 cgtaaacttc gatgatgttt ttgaccgcgt tcaccacttc cggccagcgc ggcggattgt
    21421 tgggcagaaa cggaatggca agcttcgaga acatcggagc gctgcgggcg ttggaaacct
    21481 ttccgcccac ccagatggag atgccatcgt tgtccgggtc ggcaatgggc atggccgggc
    21541 aaaccgtgta gcagttgccg cagtacatgc atttttcatc gttgacggtg acggttttgt
    21601 ttttcatgtc gccgcggatg gcgcccgtcg ggcaggaggc cacggtcgac gggatttcgc
    21661 aaacattggg cacgcgcgaa ttgtccgtct tgggaaccgt tcggtggatg cccaaaatgg
    21721 cgatatccga gcagtggacg gcgccgcaca tattcaggca gcaggccagc gcgatgcgga
    21781 catgcgccgg aagtttggag ttcgtgaagt gatcgtaaat ttcatccatc agcgctttca
    21841 ccgggccgga ggcgtccgtg gccggcgtgt ggcaatggac ccagccctgc gtatgaacga
    21901 tgttggagat ggaatgccct gtgccgccga cggggtagcc gcgctgcttc agctcggcga
    21961 tcagcggctc gattttgtcc ttgtcgtcca gcagaaattc aatattgtga cggctggtga
    22021 aacgcaggaa gccctcgcag tgtttttccg cgatgtcgca gatttcgcgg atgtgatccg
    22081 ttgaaacgag acggggcgag ccggcgcgca cactgtaaag cgcctcacct gactgggaga
    22141 cgtgcatcaa cacgcccggt ttcaaaattt catgccagag ccatttcccg taatttctct
    22201 ggatcacggg aggaagcatg gtcttataat cagggggtcc gatatccgtt cttgcagcca
    22261 ttttacttta cctccttttt gatgtcttcg ggccagaaga aaacgtaggg gttggttctg
    22321 ggggccttca ccatctgcgg caccgcggga agaccgacgg ccttcaaaaa gacccgcatg
    22381 cccaggcgct gaatcagttc acctaagcgt tcacgcgatt tgccgaactc atcccaccag
    22441 tcctgaatac gctcgatcag atccttcagt tcatcatagg gcggttcgag tttcatgaac
    22501 ggtacgacca cccaggagag cagggcgccc cggacaatgg gcgctttgcc gccgaccaga
    22561 atagacgccc cgcgttcttt gcccggcctc aaggccttcg gcatcacgtt gatgcagtgc
    22621 atgcagcggg tgcagtcttc atcgtaaatt ttgagttccc tgccgtttaa ttccatgcag
    22681 cgcgtcgggc acagatcgca gacttctttc tgaatgttga tcgtttccgc gtaagcggcg
    22741 acttccttct gatcaatttg aatggcatcc ttccaggttc cgatgatgga caggtcggac
    22801 cgggcgatgg aagccacgca gtcgttggcg cacgcggaaa tcttgatttt gaatttataa
    22861 gggaacatcg gacgatgcat cttgtcctga taggtcatgg tgacgttgta gcagatgtcg
    22921 aggctgtcga tgcaggccca ctcacagcgc gccggcccca cgcaggcgct gggggtgcgc
    22981 agcgccgatc cggatccgcc caggtcaaac ccggcgtccg acagatcgtt aaagcagggc
    23041 tgaagatgct cggtggtggt gcccaacaga atggcgtccc ccgtggatcc gtgcatgttg
    23101 gtcagtccgc tgccgtgctt gtcccagatg tcgcagagtt tgcgcagcgt gtccgttttg
    23161 taaaaccatc cggtcgggtg attgacccgc atggtgtgaa acgcctgcac gttgggaaac
    23221 aaatccggac ggtccgaata ccggccgatc acgcccgagc cgtagccctt gacgccgaca
    23281 atgcctccat gtttccagtg gccgatttta tctttgtagg aaagttccag ctgatgcaga
    23341 agatccttgg ccatcggatt cttctgcgct gcttctttca tgtctttgac gaagctgggc
    23401 cacttccctt tttccagctc atccagcagg ggggtgtctg tgtttgccat tttagtcctc
    23461 cttcttatca atattctaaa tcttcaggag tgttgatcct atttattaga ataccattca
    23521 caacttgaat agtactcttt ttatgaagta tgtcaagttt tattttgccc ggatcggaaa
    23581 cgggtcattc ggccattatg atgaattaat tcaaatgttt ttcttttctg cgcaagacag
    23641 gggcgcgcct tccgcattcg aaaaacaggg tattttgcgc gtttgtgctt tgtccgtgga
    23701 aggctccatt tcctgattga tatgctgtga aaagttgaca cgacaacgac gcgcccccgc
    23761 gacggctttc cttgatttat atgcgaaagc cgccgcccgg ggcgatcgcc gctttaagtt
    23821 accgacaggc tgttagtgcc cggccgggaa aaacgtgtag ctcacccaca ccagaacgac
    23881 cagcagcacg acgccgagaa taagactcca gccgatcagt ttcttttcga tcggcagtag
    23941 gggttcatat tccatttttt ccatttcttc ttttattttt gtctctgtca tgatgcgctc
    24001 cttattcatg ttaaattatt gaaccagcgg cggtacaacg ccctggaaga agatccacga
    24061 gatgatcaac cccacccaga tgatgaagcc gaacagcgcg atgatgtaca ccagcatcag
    24121 cttgccgatg ccttcctccc agagcttctt gaaattggaa gccagcccga tcgagaagaa
    24181 cgtcagcgcg aagaagagct tccggaacac atcgctttcc gccgccgaaa ttttcagctg
    24241 cgacttgatg tccggccagt tgagtccgat gatcagcatg atcagaaacg tgacgaagta
    24301 gccgatgacg aatttgggga agcggtccca tatttccccg gctttggcct ctacgccctg
    24361 cttgggattg atcttcgtcg tccagatcac ggccagaata aacgcccaga tgccgatgaa
    24421 gatatcgatg aagattttca cggtcgtggc ggcgccgacc acccatccgg gctggtaatt
    24481 gatgccctgc gccgcgttgc gcgcgtaaat cagcgattcg acgatctggc cgctcgccac
    24541 cgccgcgccg tccgtcttca ccgcgagccc catccaggcg ccggccacca gcggctgatt
    24601 ggaaagaaat tccgtcgcga caaaggggag aatgatcagt tcgaccacgg cgaaaatgac
    24661 gaccagcgac gagaccatga tcggcacgat cggccgggct cggatggcgc cgcccgtcgc
    24721 aatcgaggcg gaaacgccgc agatggaaat gccggaggcc agcggcgccg accattcacg
    24781 gctgaacttg aagaatttcc gggcgatcag ataaaccacg gcccagtaaa tgaggtaggc
    24841 ttcaatgatg gcgcaaaggc cccggaacat catcgacgtc gccagcccca gttgctcggc
    24901 cgatttcacg ccaaggccga cgcccatgat cacgatggcc gttttgatga accattccgg
    24961 ccgggccgct tccttaagcg aatcggcgaa cttcggcatg aagttgccga tgaagaggcc
    25021 gaccagcagc gccagaatca gccccgcctc accggtcaat ttcagcgacc agtccagctc
    25081 gaattttttc atttccgcgg gcgtctgcgc ggcgatgacg gcataatgtc cgacaatcgt
    25141 acagatgtag gtcagccaga aaatcaccgt aaagccgtaa ataaattttt tcagattgta
    25201 ccccagggct ttggcgccca ccagtaggac cgccatcacg gccagatagg ttaaaatgat
    25261 ggatatcaaa ggatggagcc ccgcgtaggc tttggatgca ggcgcgatcg atttggaaaa
    25321 cgccgtccaa actttggggg tgacggccca ccccagaaga tccagcccta ccagtgagcc
    25381 aagcgagaga ataaaaataa acagccccag ccacaccgac caccagtctt cggtctttaa
    25441 aagaccttgt ttgttgccca taacgacctc ctttattata aacggtttaa tcatggcttg
    25501 cggagcgagg gaaacatctt tcccgcctct gcaaaaatca atacgcctgc tgccttcaaa
    25561 cggaaattga tgaattcctt ttgcgcccgg tccgccgatt ttgcgtttga gcctgcaagc
    25621 aatttttatc aggccatacc cgccggactg ttagccgttg ccggttgcgc ttcgggcgaa
    25681 accctcctta aggtatggat gcaatggatt taaagtcagc gccggaaatt gtcttgaagc
    25741 gtttgagaaa gcggagaaaa aaaacgagca aagagatgcc ggaaaatacc gtcgaatttt
    25801 cctgatgccg gatggtgtct caaaaaccgt cataaaactc tcccacgggc tggaagttca
    25861 cagatcagtt tcgtcaacga ttcatgccaa taagcctcat atatcagaga tggccttgaa
    25921 tgtcaaaaag aaacagaagg aaagacggcc aatgtcccgg cagcggtccc cccctcccca
    25981 cacaatattt caccttgact ttttcttgcc tgtctataca gtctttccaa gaaaactttg
    26041 gacagccgat gatcgaaaat ctaatcagca acttatccgc caacttgcag ggttccgtcg
    26101 gactttcctt cgtggccgct tatctgggcg gcgtgctgat cagttttacc ccctgcacgt
    26161 atccgctgat ccctcttacg gtcggcttca tcggcgccca gggatccgga tcgaagctgc
    26221 gcggatttct gctgtctgtg ttgtatgtgt tggggctttc cgtcacctac gccgcgctgg
    26281 gggcggcggc ggcctggtcg ggaagactgt tcggacagat gcagacgacg ccgctcgtgt
    26341 attggatcat ggccaacatt tgtctggtga tggggttggc catgctcgat gtttttaaaa
    26401 tttctcttcc cgtgccgcaa aaaatggcgg cctattccgg cggcgggagg aagggcttcc
    26461 tccccagctt tctgttgggc gcggcttccg ggtttgtgat cggcccctgc accgcgccgg
    26521 tgctgggggt gttgctcggt tttgtggcgc tgaaaaccca tgtggtcatg ggcatcggcc
    26581 tgttgtttgt ttttgccttc ggcatgggga cgctcctgat tctggccggc acgtttgccg
    26641 gcctgattgc ggcgcttccg cggtccggcc gatggatgac gatggtcacc agggctttcg
    26701 gtgttatttt gattggcgcg gccgaatatt ttctgtatac tgccggcaca ctttcttttt
    26761 agcgaaaagg gtcatcatgt acaaaaacag cattgtcctg gcgctggttt tctttctggt
    26821 tctggccgtt gtgtcggctt cccggggtca gtatattccc aacgcgccgt tcaagccgca
    26881 ggtttttgcc gctccggatt tcaccctcaa agacctgcag ggcaaaacct ttcgcatgag
    26941 cgcccagcgc ggcaagccgg tgctgatctt tttcggaacc acctggtgtc cggggtgcag
    27001 ggccgagatt ccaaaatata aaaaggttta cgagacgtat tcgcccaaag gactggaagt
    27061 gatttacatc aacatcatgg agccggccaa aaaagtcgcg cgattcgccc aggccaatgc
    27121 cctgccttac cggacgctgc tggatgagga cggatccgtc ggcacggcgt acaacgcgat
    27181 cggtgttccc atgatgatcc tggtggataa agacggcgcc gttgtgaaag tcggcttcag
    27241 ctcctcgcaa atgccgttgg acaaagtgtt gccggtaaaa taaaatacca cgtgttttcc
    27301 ggcgcggtgt gtgtccggaa ggcgaccgga cgccatcgat tcccctgttg aaggatggac
    27361 ccatgaccac aagcaactgg tttgccgaat tttccaagtc cgaaaccgat cgctggatcg
    27421 gcggtgtctg cggcggtttg ggagagcaca cgcccgttcc ttcgtgggtg tggcgtttgc
    27481 tgtttgccct gctggctgtt ggattcggga ccggattcct tctctacctt ctgctttgga
    27541 ttttcgttcc acgcaaggcc caaacagggt gaagccgaac agagatcaaa ggattttttg
    27601 aaacaggaaa gggttaacca tgaaatttcg ggacgatatt tatatttatg agtgggcaaa
    27661 ctattttgac aacaactgca acagctatta catcggcggc ggcgtacagg cgctgattga
    27721 tcccggcctc acccgctatc tgccggacct gctcaaccag atggcgaatg acggcattaa
    27781 gaagaaagac atccggtatg tgatcaacac gcattcgcat cccgatcatt ttcagggctc
    27841 cgagcttttt gaccaggaaa aggtaaaaat cgccctgcac cgcaaggaaa tggacttcct
    27901 caaaggcgtc ggcggcgagc tgtacggtct gttcggcatg acgattccgc aaatgcagat
    27961 taacctgcaa ctggaggacg gccggctcga actgggcgat cagacctttc gggtcatttc
    28021 cattcccgga cacacgccgg ggtctatcgg tctttactgg ccggcaaaga aagtcctctt
    28081 ctgcggcgat gtggtatttc agcagaacgt cggccgcacg gattttcccg gcggcaacgg
    28141 cgctctgttg aaaaggagca tcgtggcgct gtccgatctg gacatcgatc tgcttctgcc
    28201 cggccacatg ggcattatcg aggggcacga caaagttcag gacaatttca acattattat
    28261 tcagaacatt tttccctaca tttaaggagg gcaaacgcag ggagcggccg gcggccgttt
    28321 tcctgagaac gggggggatc ggcgccgcat ggccaatgag ggaaacaaag tcatctactc
    28381 catgatcgga gtcagcaagg tttacgacaa gaaacccgtg ctgaaggaca tttatctttc
    28441 gtatttctac ggcgtgaaga tcggcgtgat cggtctgaac ggttcgggca aaagctcgct
    28501 tttgaaaatt ctcgccggtg tggatgatga atttctgggg aagaccattc tttcccccgg
    28561 atacacggtg ggctatctgg agcaggagcc gcggctggac gaagacaaaa ccgtccgccg
    28621 gattgtggaa cagggcgttc aggccaccat ggatgtggtc aacgaataca acgccgtcaa
    28681 cgaaaaattc tccgaagaaa tgtccgagga ggccatggcc aaactggtgg accggcaggc
    28741 cgcgctgcag gaaaaaatgg acgcgctcga cgcctgggac atcgactcgc gcctggagat
    28801 ggccatggac gctctgcgct gcccgccggg cgatacgccc gtaaaaattc tgtccggcgg
    28861 agagaggcgc cgcgttgcgc tctgccgcct gctcctgcag aaacccgata ttctgctgct
    28921 ggacgagccg accaaccatc tggacgccga atccgtagcc tggctggaac accacctgca
    28981 gaaatacgaa gggaccatca tcgctgtcac gcacgaccgc tacttcctcg ataacgtcgc
    29041 gggctggatc ctggagctgg accgcggcca gggcattccc tggcagggca attattcttc
    29101 ctggctggaa cagaagcaaa accgcctgaa aagcgaggag aagtcggaaa gcgccagaat
    29161 caaaaccctg gagcgagaac tggaatggat ccgcatgtcc cccaaaggac ggcatgccaa
    29221 gtccaaggcc cgcatcaaag cgtatgaagc actgctggac agggatctgg aaagggaatc
    29281 cggcacacgc gaaatcttta tcacgcccgg gccccgcctg ggcgacgtgg tgatcgaagc
    29341 cgagggggtt tccaagggct acggcgaaaa gctgctggtt gaaggcatga cgttttcgct
    29401 gccgcccggc ggcatcgtcg gcgtcatcgg ccccaacggc gcgggcaaga ccacgctgtt
    29461 ccgcatgatc accggccagg aaagccccga tgcgggaacc atccgcatcg gcgagaccgt
    29521 caagctggcc tacgtcgatc agagccgcga cgcgctcgat ccggagaaga acatctggga
    29581 gatgatttcc gacggccagg acatcatccc ggtcggcggc cggcagatca attcgcgcgc
    29641 gtatgtgtcg cgcttcaact tttccgggac ggaccagcag aaaaaagtgg gaacgctttc
    29701 cggcggcgag cgaaaccgcg tacatctggc gcgcatgctc aaggaaggcg gaaatgttct
    29761 gctgctggac gagccgacca acgacctgga cgtgaacacg ttgcgcgcgc tggaagacgc
    29821 gctggagaat ttcgcgggct gcgttgttgt gatcagtcac gaccgctggt ttctggaccg
    29881 catctgcacc cacatcctgg cctttgaggg agacagcaaa gtgctttttt tcgacggcaa
    29941 ctattccgaa tacgaagaag accgcaaaag ccggctgggc gcggccgcca gccagcccca
    30001 ccggatcaag taccggcagc ttaccagacg atagccgccg gctgcgcaag cccgagcagg
    30061 ttttttggcc gcaggatctt ttgcgccatc cctataaagg agatcgatat gaaaaaaata
    30121 tggacgacgt tgacggcggc ggttctgttg ttttcctgct tgccgccggc gcaggcgcag
    30181 gaatacggaa aggtccgtgc gcttcaggag cgggcggcgt atgtcacccg gcagaaaaat
    30241 gattttgtcg tgcgggtttt gcgttcttac gagattccgc atgaggtcaa cgatcagggc
    30301 gtcgttgtcc gcatcaacat gggcggccga tggatggacg tcacgtccat tgaaatcgtc
    30361 cccgtgctcc gggaagccga agaccaaagc cggcaggtgg ccgcacacga gttgtttttc
    30421 tttacgacgg acgggatcct ggatgtggtg tcggcgctta ccatccggta ggtttatttg
    30481 ccgagcgtcc ttttcatttc cgcgagcttc tcttggtcca gatagtattt gccgtcggac
    30541 gtcctgacga ccagtttgtc cgatatcaga aggtgaagcg cccgggggcg caaattgccc
    30601 gtgccgaccc gaaagaaatt tttacgctca tcctgaagtt tcatggccct ggacgccttg
    30661 acggcgcccg cctcttccag ctcggcaatg attttgaagc atacccgccg caggcccaac
    30721 cccgtcaaat acatcgccgc cagaacgaaa accataaagc cggcaaccaa caaaaggatt
    30781 tgcgcataaa caggcatatg aatatttttt atgccggaat cgggaagccc ggcctccgga
    30841 cttttgagat ggccgttaaa taaacttcac ttcacgcgtc gctttcgtga ccacataaca
    30901 gcgctctaca ttggactggg agtccgccgg aatcatgaaa aaacccggcc ggttcaactg
    30961 atacccctgg gtcgttccga tcatttcctc tccgtccttg aagagcaccc ggatcttgcg
    31021 gcccaaaacg ggtttaccgg gttcaaaatt cttcttgtcc tcatattttg gattcccggc
    31081 gaaatccttg acgaaaaaaa cggctttcag gtcggggatg cgcacttcca gcggtttgtc
    31141 gctgggtgaa gaccccaccg gcgcgatgtg aaaaatatcc cggttggggg caaagtcaac
    31201 cgtgacgcct tttacaatcc gcttgtcggc atagcggatg acaatcttgt tcatcatggc
    31261 ggcaggctcc tttccggcgt gagccatttt ttcaacgagc agttttaccg gacatctttt
    31321 ctattcttac accaaccacc agaactttgt ccagtgcgtc atcggcataa acaaaggatt
    31381 ctttccccga atattttttc atcaggagat cgagcgcctt cttcttttct ccggcctttt
    31441 cgataaaata tgcctgaccg aaaccgatca cactgcggta tttcatgccc cacacgcagg
    31501 cggtttgacc ccgcaccagc tcgcccggga tatccacttc aaagcatacc gacgcattgg
    31561 ccttcagcat gtcgatcttt ttgccggatt tggcgcaatg aaaataaaga cgggaatttc
    31621 ggaccgcaaa agtgaccgga acgatgtagg ggcgatcgcc ttcgcacatc gcgatccggc
    31681 aaaccgctgc gctctgtaaa atcgcgtcaa tttcgccggg atcctgaatt tctttctccg
    31741 accgaatcat ggcgctgccc cctctcctca tacggcaaat tcaaatcttt cgaagaactc
    31801 ttggcatgtt tcggaaagtt atgatacaaa aaccccgctt tcttgtctgc ttttccagat
    31861 gctgctttta tccgaaacgg ccggaaaagt cgatgattta atgatcggga tacgagagaa
    31921 gaggatgaac atccagaccc atcgggcgat cggcgttttt gattccggca tcggcggact
    31981 gaccgtggtg cgttctctca tggaacggct gccctttgaa aatatcgttt atttcgggga
    32041 tacggcgcgt gtgccttacg gcatcaaatc ggtagagacc atcaaccgtt acgcgctgca
    32101 aattactgaa tttttgttga agaaggatgt caagctgctg atcgtcgcct gcaacaccat
    32161 ggcggcggtc gcctatcagg cgatccggga tctttcgccc gtgccggtgc tggaagtgat
    32221 cgaggccagc gcaaaaatcg ccgttgaccg gacgcgcagc aaagccatcg gcgtgatcgg
    32281 aacgcccgcc accatcaact cgaacgccta tgccagagcc attcatcttt tggaccggga
    32341 cgcgaaggtt ttttcgcagg cgtgccccct gtttgttccg ctggtggagg aaggctggtt
    32401 tgatcatccg gccacccggc tcgtcgccga ggaatactta aaacccgtca tggccgagcg
    32461 cgtggatacg ctggtcctcg gctgcacgca ttatccgctg ctcaaacccc tctttcagga
    32521 catcacgggc gggcaggtcc ggctgatcga ttcagcggaa gccatggcgg acattgccgc
    32581 cgacctgatc aaacaaaaaa atctgggcaa cacgggccgc cggccgccgg agtatctttt
    32641 ttgcgtcagc gatgtgccgt accgttttca gaccatcggt gaacgctttc tcggccggac
    32701 gctgcctcga gtggaaatgg tgaggcttca atgatgaagg tgctgctggc cggctacaac
    32761 gtcgattacg atttgatccg cgaccttcgt gagaagagcg ggctcaccca ggacattacg
    32821 ccggaaacga tttccgccgc ctacgcccgc atcagccgca gtcccaaatc cgtcgatcag
    32881 ctgcgcgttg acgcccgggc cgaagtcgaa aaagcccgcc gatccaaccg gaatatcgtt
    32941 tttgaaatgg ggcacagttc ggtagccgaa cacgccgtct tcaatatcga cgtcatcggc
    33001 gtttcccgtc tgctggtcga agaaatcgaa aagttccgcc tgtgctccta tacggaaaaa
    33061 tcccagcggt acgttttgtt tgaccgggac ttcgttgtgc ccgaagaaat tgtccgggcg
    33121 gggatggaga aggattttgt cgaaacggtc cggatgcaaa acgattttta ccatgagctc
    33181 tacgagtggc ttcggccctg ggtctttgag caaaaccggg aactggccga aaatcccgcc
    33241 aaccgctctc tgctggaggg ctgggccaaa gaagacgcgc gctacgccat agcgctggcc
    33301 acccggacgc agctgggcat gacgatcaac gcccgcaatc tggagctgat gttgcgccgg
    33361 ctggcctccc tgccgctcgc ggaggcgcgc ctttgcagcg aaaagctcta tgccgcaacc
    33421 cgggacatcg cgccgtcgct gatccgctac acccgggcga cggattacga ccgtctgacc
    33481 cggccgcggc tgcggacaaa ggccgaaatg cttctggcca agcggcctca agaggcacca
    33541 tcgggaaagt tcgccgacgt tcgcctcgtg tctgcgacgc ccggcgcgga ccggcaggcc
    33601 gccgccgcgc ttttgtttgc gtcttccggc ctgagctacg gacagtgcca aagccgttct
    33661 gcccggctga accaccgcga gctgaaaggt ttgttccaaa ccgtgtttga caatatgcaa
    33721 ccctacgatg cagcgcttcg ggaactggag aatatcgatc tgcagttcga gttggtggtc
    33781 agcgcgggct gctttgccca gttgaaacgc caccgcatgg cgaccatcat cgcccaggac
    33841 tacgaccccc ggctgggggt gaccgttcca ccctccgtgc gcgccgtcgg ccggcaaaag
    33901 aaattcatga ccgtcatgga ggcgacccaa agggcgcatg atcgcatccg gaacaaactg
    33961 ccgtcggcgc cgccgtatct gctgaccaac gcacaccgca ggcgggtgct catgaaattc
    34021 aacgcccggg agctttatca tctttcccgc ctgcgggcgg acgcgcacgc ccagtgggat
    34081 atccgaaatc tttcggaaaa aatgctccgg caggcgcgcc gcctcatgcc gctgacgctg
    34141 atgatggcct gcggcaagga cggctttgcc gcgctcactc aaaaagtttt ccccggggca
    34201 tagccctctt tcctctggcg ggaccccatg acctatcaaa gaaaatggcc cattgtcctt
    34261 ctggccacca ccatcagctc gtttacgacg cccttcatgg ggtcgtccgt gaatgtcgcc
    34321 cttcccgcca tcggccggga tttttcgatg gacgcgcttt ccttaagctg ggtggcgtct
    34381 tcctttcttc tggccgcggc tatcatgctg gtgccgctgg gtcgtctggc cgatcttttc
    34441 ggacgcagaa ttttttttct ttcgggatcg ctgatttttt ccctgtcctc gctgttgagc
    34501 atctggccgg aaacggaaag cctttttatt gctctgcgcg tggttcaggg aatcggcgcc
    34561 gcgatggtct tcagcaccgg cacagcgctc ctggtttccg cctatcctgc gggcgagcgc
    34621 ggcaagatgc tgggaatcaa tattgccgcg gtttacaccg gcctcaccgc cggtcctttt
    34681 atcggcggtc tgctgaccca gcatctggga tggcaagcca tttttatcgt taatgccgca
    34741 ttgggtttgg ccgccgctgt gctggccgtc cgactggaaa aggaagcgcc gcccgccgcg
    34801 gcgggtgaat cttttgatct gggcggatct tttctgtatg cgacggcgct ttttgccctg
    34861 atgtacggtt tttcccggtt gccgtcttta agcggcgccg ttctgatcgc cgggggcggt
    34921 gtctgtttca tggtcttcat tcgcgggcaa ctgaaaaagc gctctgggct catcaatctt
    34981 cacctgttta tgggcaaccg ggtttttgct ttttccaatc ttgccgcttt gattcattat
    35041 tgcgccacct tcgcgatcac tttcctgctg agtctttatc tgcagcacgt caagggactt
    35101 tcgccttccc aggccggctt tgttctggtc atcgaacccc tgctgcaggc ggtcttctct
    35161 ccgctggccg ggcggctgtc cgatcgctac gagccccgca tcatttcttc catcggcatg
    35221 tcgatcacgg tggtcggcct tgccggattg atttttatcg cttcggatac gccgctttac
    35281 ggcattgtat gctgtctggc tttgctgggg atcgggtttg cgcttttttc ttctcccaac
    35341 gtcaatgccg ttatgagttc cgtggaagac agattttacg gcctcgcatc cgcgacgcag
    35401 gcgaccatgc ggatggtggg gcaaatgctg agcatgggca ttgccatgct ggtttttgcc
    35461 tggatcatcg gcaaccacgg catcgaccat gagaacggcg cgctgttgat ggtcagcgcg
    35521 aagagcattt ttgcgattct ggccgccatc tgcacggcgg gcgtctttgc ttcgctggcg
    35581 cgggggcgca tacataaggc ttgattttca gtctgcgatc cgtatgattc gggtcggcga
    35641 cgccttttat ttttgtgagc ggggggaaca atgaaggcac aggggatcag ctttgccatc
    35701 ggtcttgccg cgggctgttt cggcggcctg atcggactgg gcggcggaat catcatggtc
    35761 cccctgctgg tggaactgct ccatttggat cagtgccgcg cgcacggcac gtccctggtg
    35821 gttttgattt ttacgggaat cagcgggggc atctcttacg gactgcacgg ccaggtggat
    35881 tggaaggcgg ccgtcctgct ggcggttccg gccatgctgc tggccgcggc gggcgcctgc
    35941 ctggccgacc ggctgccgga ttggaaatta aaaaaaattt tcggggcgtt cttaattttc
    36001 tgctccgcgc ttttgctttt gaagcccgtt cttgccgatt ggatcggcgt cctgcccgat
    36061 gcggtttccg acattgccct ggtggcgacg ggcgccgcca cgggatttct ttccggactg
    36121 atggggatcg gcggcggcac gatcatgatt cccgccatga tcctgctggg aggctttacg
    36181 cagcacatcg cgcagggaac cgccctgctg gtcatggtcc cctccggatt cgtcggcgct
    36241 ttcacgcact ggaagctgga caatgtggcg gtcgaactgc ttttcagcat ggtgccgggc
    36301 attgtgctgg gcgcttttgc cggcgccggc atcgctcagt ttattccgga ggcgcctctg
    36361 cgctggatgt ttgtcctggt ggcggtttat atgggatggc gctacgtgaa agttgtctca
    36421 ccggatacgt gctgaatcgc cggattgtgg ttttcgggct caaaaaagct ctgatggatt
    36481 caggcggatt ctccgcggaa tatcctcgtt gctgccggaa caaaacgctc ttaaggcaag
    36541 cgaaaaggat attttgggca accgcccgtt ttaatcttac gccttctttc cgtattccgg
    36601 atcgattttg accagcgccg tcaccatgaa gcccgggatg gtcgtgagca atatccagta
    36661 gtagaagtga atatagccca gtttttcctg gatccagccg cttgacatgg ccggaaacat
    36721 catgcccagc gccatgattc ccgtgccgat ggcgtaaaag acggttttgt attcgccctg
    36781 cgacaccatg atcatgaaca tagtgtaggc ggtgaagccg aagccgtagc cgaactgctc
    36841 aaaggccacg gcggagccga tgatccagag attctccggc tgcgcgtgcg acagatatac
    36901 aaaaaccaaa tccggcagat gcatgacaat caccatcggc cagagccaga atttcaagcc
    36961 gttgcggtaa atgacatacc cgccgagcaa tccccccagc atcagcgcga ccacgcccac
    37021 cgtgccgtaa atccagccga cttcggcggt ggaaagcccc agcccgcctt tttcccgcgc
    37081 gtccagaaga aagggcgcga ccatttttac cagctgcgct tcggcaaacc ggtagaacaa
    37141 aaagaagcag acaatgatga gaatatcctt ctgccggaaa aagagggtga tgatccggaa
    37201 atactcggcc accatcccgc gccctctgtt cagcggcgcg ctgtggtccg cttcggggcg
    37261 gggcaggatc aggaaatgat acaggcacaa cgccagaaaa aaggccccga cgaccacaaa
    37321 cgtgaccgac caggcggaag caaccggata gccatgctga ctcaacgtgc cggccaggat
    37381 gaccagaccg cccttggaag cgatggtggc cacgcggtaa aaaacggtgc gcacaccgac
    37441 aaaagcggcc tgctcggggg tggaaagccc cagcatgtaa aagccgtcgg cggcgatgtc
    37501 gtgggtggcg gaagaaaaag ccatgatcca gaacatggcc agcgtatagc ggacaaaatc
    37561 gcttgcgggc agcgtcaggg cgacgcaggc gagcgacccg ccgatggcca gctgcgtggc
    37621 gatgatccag aaacgcttgg tgcgcagaag atcgacaaaa ggactccaga ggggtttgat
    37681 gacccagggc aggtacagcc agctcgtata aagggcgatg tcggtattgg aaagtccaag
    37741 gtttttatac atcacgacgc tgacggtcat gacgatcata tagggaattc cctcggcgat
    37801 gtagatggac gggacccaga gccagggggt gcgcttcgct ccttttggtt ttcggtcgga
    37861 agggggcatg atgttccttt atctacttct tacgagcctt tttcagttcc tcattgatga
    37921 accaggtttt gattttgtaa tagttgcaaa gccagacggc atatttcaat ttttcccgat
    37981 ggccgggttc atcctccagg atatagaaag cgacggccgg ttttttcccg gtttccttgg
    38041 catagtgcag cgcctgcccg atggcttcat gaaaattttc cgggctgtcc acttcaatgg
    38101 cgtactcgct gctcagcaga tcaatgcggc cgtatttgac cggcgcctcc atctgcgcct
    38161 caccttggaa atagacgtcg ttgaacgccc gctgaaagtc ctgttccgag cgcaaaacat
    38221 acttctggga agcgccgcag gcggccagaa ccatgatcag caaaatcgcc gcaaggaggc
    38281 cgccgtatct tttgaggagc cgagtcttcc tgttcaatac aatttcctca attgtgctcc
    38341 ggcaaagtag tgcgccagga tttcttccgc ccggaagcct ctgacggcca tgaccgccgc
    38401 gccgatctgg cagaggccca cgccgtgccc ccatccgccg ccctccagaa gaaaattctg
    38461 cgcggttccg tccggatccc gctcgaccga gacgacaaac gcgctggaga gcaaatgcga
    38521 gggcgaaagc cagcggcgga tttccagctc cttgccgacc acgaccgtgc ggcgcgatcc
    38581 tacgactttc agccggaaga tccttccgga aggcccccgc gccagcggct cgaggttttg
    38641 cagggtgccg aaatcgatgc cggacctggc ccggagaatc tgctccagct cctgccggct
    38701 gtaggcgacc cgccagcggt agaagttcag ggtctccagg tcaaaggaag gcaggatcct
    38761 ttgcagaagg ttctgatcgg cggtgttgca gaaagcgtcg ggacgggagc gaagccaccc
    38821 ggccgcgtcc tgttccgtgg cgatcggcgc ccccggccgg tcagcgtcag ccacgccgga
    38881 aagataggga aaggatgcat cttcccaggc ggttgcgtaa acatccgtcc gtccgccgca
    38941 gcatttgtag tagcgcgcgt cgcagatttg gtctccgctg gtgagaaaca cgccccgcgt
    39001 ggccccgatg gcccgggcaa cattttccga agcgatgcgc gaaatgccct gataccgctg
    39061 gcagtggtcg tcggcgcaca catcgaagcc ttcgtggtcg ttgacgtcct gccagacgga
    39121 gatttcatcc tgccggtttg ggggcggaag cctgtgggtt ttcttccggt ccagcatcgc
    39181 gaccagccag ctgcgggacg tgacggcctg cgccttgaga aactccaggg gggcgccggc
    39241 cgacatttcc gacgagatca ccgaagcgag gtattcttcc agcgggacaa tattgatgag
    39301 gtgaaaggca gtcgggcttt gtcccgacag aaaaagcgag cccctaaacg attgtttctg
    39361 aacgcgctgc cagtgaaaat cgatgccgat ggttacgtcg gggacggtaa aaaaagattc
    39421 ggcgggcatt gcgggggaaa gcagaaggtt tttgccctgg aaaatttctc tgccgtcgga
    39481 gtcggtcaga acgatcaaac catcggctcc acgggctgtg tgctctccgt tgatgccgtg
    39541 tccgtcggga agaacaaaaa cgccttccag gatcatccgg gcttcggaag tgtgttgcag
    39601 cagggccgcc tgtatgtccg gttcgcgatc gatcattcct tcatccccgc cgaaaagtta
    39661 tcggcctttt tagaaacaat gggtggagaa gtcaagaaag ttgtcgctgc ctgcgctttg
    39721 gtcttacggg aaaattcgtt ttgcttgggc ccggcggggt gaaaattttt taaaatgggt
    39781 aaaatcatta gcaatatcaa actttttgct tgaatcttgg cggtttatcg tgtagaaaaa
    39841 taccaaaatc actgagcgga tgggttatgg cgcacatttg gccgattggc ggaggcaagg
    39901 gcggttccgg caaaagcttt atcaccagtt cgctgggacg gttatcggcg gggttgggca
    39961 ggagaacgct ggtaatcgat ttggacctgg gcgcggccaa tctgcatacg ctggtcggtg
    40021 ttccctatcc ctcaaaaagt ttctccgatt ttctccagaa aaaagtgacc cgactcgacg
    40081 atacggttgt ggagacgccc tttgaaaatc tcttcctcat cagcggcgct caggatcctt
    40141 tcgatattgc caatcttccc tatgaacaga aaatcaaggc gctgcgcgcc atcatgagac
    40201 tggactatgc ctgcatcctg ctggatctgg gcgccgggac gtctttcaat acgctcgatt
    40261 tttttctgct ttcccaaaac ggcattttta tcaccacgcc ggagccgacc tccatcgaga
    40321 atgtctatcg gctgatccgt tccgtttatt tccggcggat tcgctactgc ttcagcgtgt
    40381 cgcagttcaa agcgctggaa gaagaggcga tcgagcagtt cggcgaaggc gccttcaata
    40441 agccggagtg catgatgaag gtcattgagc gggaccaccc ggaatcctac agggagctgc
    40501 agaatgattt taacgccttc cggttcaggc tcgtcatcaa ccaactgcac aagcgcgaca
    40561 acgccgttct cgggttacat atttgcagaa tcatcgaaaa gcacatgggg ctgggcattg
    40621 aattcacggg caatgtttct tacgacgatc atgttcacga tgcgatttgt cagcaggtgt
    40681 cttttctgga ccggtatcct tacacaaaga ccgcgggcga actgcgcgcc gtgggccgtc
    40741 agatctttca gccggcgggc cggcagttga tgcttcagta tgtctagaga agattctttc
    40801 aggtgcaata cggccaaagg ggcttgtatg cggaaaagtt tcgccgagtt aaactattat
    40861 gaaatgctgg acatccggcc ggacgctgcg gcatttgaaa tccgccaggc ctacgatgcc
    40921 gccctgcaga tttaccagcc cggttcgctg gtaagccatt cttttttcac cgacgcggaa
    40981 aggcgggaaa tcctgtccct ggttgaaaaa gcctaccgga ccctgattca ggaccagctc
    41041 cggaaagcct atgatgatga actggttcgc cggggggagc tcgacgccgt aagggaaacc
    41101 gcgcctgcag tcaaaaagcc ggtcggcatt ttcagtatca gccgcggtcg cggccggcag
    41161 ccggccgggc ccaatccaga atccctgaaa aacaggatcc ttcaaagcgc acggattcgg
    41221 gagctgacgg gtcgccccgc attgagcggc gaagatctga agcaaatccg cgaggagctt
    41281 gatgtgacgc ttgaggaaat ttcgcaggca acgaaaatca ggcaggatca tctgatcaat
    41341 atcgaggagg attgctttgc gaggctgccc gcggctgttt tcctcaaggg gtttgtcaag
    41401 tcgtatttga aatatttgtg tttgaaaccg gcagaggaat tagccgcccg ctatatggag
    41461 actttcgcgc gcctgtcgca aagtcaaagg gcggaggaaa ataaatgaaa ggagcgacca
    41521 tggaattcga tacgattaca acagcggagg gggatctggg catccggttt ctggggcaca
    41581 gctcgctcat ttttttctgg gcaggcaaaa ccattcatgt ggacccggta tcgggggagg
    41641 cggattacac gaaattgccc aaggcggaac tgatcttgat tacccacgaa cattatgatc
    41701 atctggatct gaaagccgtg gacatcatta gaaccgcgga gacaaaaatc gtcggcaacg
    41761 gcgaggtcgg ccaacagatt cccggaacga ttgttctgaa aaacggcgac acgcggatgg
    41821 ttgacgggct gaagatcgaa gccgtgccgg cttacaatct tcagcatatg agcacgccgg
    41881 ggaacccctt tcacccgaag gggatcggga acggctatat cgtcaccttc ggcgatctgc
    41941 gcctgtatat tgccggtgac acggaaaata ttccggagat gagacggctt cggaatatcg
    42001 acattgcctt tctgccgatg aatctgcctt ataccatgac gccggagatg gtcgccgatg
    42061 ccgcgcgaat gtttcgtccg aaaatcctgt acccttatca tcagggcgag acggatacgg
    42121 cgaaactgct tgcgctgatg aaggatgaaa aggacatcga cgttcgcatc cggaccatga
    42181 agtgacgggg tgcgcgtcag ccggcgcggg cttgaaagac ccacgcgaag gcccggagat
    42241 ggagaaccgg tttgcatcat tactgttttg tcatcgcgag gagcgaagcg acatggcgat
    42301 ctcttccaat atccaatgct gatgagatgg cttcacttcg ttcgcgatga cagacagaca
    42361 tatgcaggtt tcagatggag ggagatgctt cagtgaacaa aaacggtatt gccttttgga
    42421 aagccagcgg cagcggcaat gattttatca tcatcgacaa ccgcgattta tcgctgaacg
    42481 tgggggaact gccggctttt gcccgaagcg tgtgccgaag aaaagtatcg gtcggcgcgg
    42541 acggtctgct gctcgtcgag ccctcgtcgg ccgccgattt ccgatggcgg tttttcaatg
    42601 ccgacggctc cgcggcggcc atgtgcggca acgcggcccg ctgtgtggcc cgttttgcct
    42661 ttgaaaacgg cattgccgga caaaccctgt cttttgaaac gcaggccggc atcatccgcg
    42721 cggaagtggc gggcgacgtc gcgaaagtcc gtctgaccga tccctcttcc ctgaagcagg
    42781 atatccgggt gcacctgtcg ggaggcgagt gcggactgga ctgcatcgat accggcgtgc
    42841 cccacgccgt ctgctttgtc gcgtccgccg aggcgtgcga tgtgacggcg accggaaggg
    42901 aaatccgccg ccatgaggtt tttcagcccg agggcgccaa cgcgaatttt gccctggtga
    42961 tcaaccggca taaaatgcgg gtccggacgt acgagcgggg cgtcgaggat gaaacgctgg
    43021 cctgcggcac gggcgtggtc gcctccgttc tggctgccgc cggacgccgt tttgtggatt
    43081 cgccggtgga tgtgatcgtg cagagcggcg aaaccctgcg cgtgtatttt acccgtcggg
    43141 acggggcttt ttcggaaatt tatcttgaag ggaaagtgaa aatagtctat caagggacgc
    43201 tttttgaaga cgcatatcga taatctctga tcaggaggga atgttcattg tgcagacggc
    43261 cgaacggtta aaaaaaatcc ccccctattt gttcatggag cttcggaaaa aaatcaacca
    43321 ggcaaaagca gacggtgtgg atgtcatttc tctggccatc ggcgatcccg tggaagcgac
    43381 tcccggttcc attatcgacg aactgtgccg gacggcccgc gacccgctga accaccgcta
    43441 tcccaccgac gaggaaaagg gcatgctcgc gctgcgcagg gaaatcgccc gctggtatgc
    43501 ccggcgctac ggtgtggagc tgaatccgga aaaggagatt ctgggcctca tcggttccaa
    43561 ggagggatgc caccactttg tcctggcgcg cgtgaatccc ggcgacatcg tgctgatgac
    43621 cgatcccggc tatccggcgt accgcgccag catcctgatg ggggaaggcg tcccctgcaa
    43681 cgtgcccatc ctgccgcaaa acgactatct tccggcgctg gaagacattc cgtcggagac
    43741 ggccaaaaag gcgtcggcca tgtttttgaa ctacccgaac aatccgacgg gcgcctgcgc
    43801 cacacaggag tttttaaaca aactggtggc gtttgcgcgc gaatacgaca ttgccgtctg
    43861 ctacgacaat ccttacggcg aaatcgtttt cgcagggcag gagcgcatca gctttcttca
    43921 ggcgccggga gcgaaagacg tcggcgtcga gctgaattcc ttttccaagc cctttaacat
    43981 gtgcggctgg cggatcggca tggcctgcgg caatcccgac ctgattgcgg caatcagcaa
    44041 ggtcaaggaa aatacggact ccggcatctt caatgccgtg cagtttgcgg cgatccaggc
    44101 gctcaccggc gaagcggggt tcattgaaga gatgctctcc atttacggac gccgccggga
    44161 gctggttttg aaaacgctcc aaaagatcgg cattgatttt cagccgccgc gcggcacgtt
    44221 ttatctgtgg gtgcccgcgc cgaaaggcat gacgtcgctg gaattcacca accgcctgtt
    44281 tgacaagacc gccgtcgtgg tggcggccgg gacggcttac ggccagtacg gcgaagggtt
    44341 catccgcatt tcgctcaccg tgccggatga tcggctccaa gaggcgatgg agcggatcga
    44401 aaaagaattt gcgccataac gctgtaggga acggtcggga ccgttcccag caggtttgag
    44461 gcagtaaaga atggaaatgg gcattctggg gcttccccaa agcgggaaga gcaccctgtt
    44521 tgaaatcatg accggcgtga aagtgagccc gtcgcacacc gaaacggttg tgcgcggaca
    44581 ggcttccgtg ccggacgcgc gcttcgaccg gctcgtcgaa atctacaaac ccgccaaagt
    44641 ctctcccgcc agggtgccgt ttatcgacgt ccacgcggtg ggcgagcatc cctgggaggc
    44701 catccgccag aatctgagcg gcgccgacgg catcctgcac gtggtggacg gtttttccct
    44761 gccggatatt tcgattgctc tcgacgccta tcggaaactc gaagatgaac tggttctctc
    44821 cgatctcctc atcatcgaaa agaagctcga acgcctggcc aggatgtcca aaaaagccat
    44881 tccggcgcag gaagcggcgc aaaacgagct tttgccccgg ctcaaagact gtctggaaga
    44941 gggacggccg ctgcgtcggg cgggccttac cgcgcaggaa gtctttacgc tcaaaagttt
    45001 ttcattctgg acgatcaaac cggaactggt cgtggtcaac gtggccgaag acaatccggc
    45061 gtttgccgat gaatttgccg aagccgccca tcttgcctgc ccggtaatgg gcatctgctg
    45121 caaggtcgag gcggagttgg ccggactcga cgcggccgac cagaaggagt ttttggcctc
    45181 gatgggcctt gcggaggccg ccttcggccg gatcatccgc gccgcctttt cgcttctggg
    45241 gcgcatctcc tttttcacgg tcggcgagga tgaagtcaag gcctgggtca ttccgcaaaa
    45301 cacaaaagcc cccaaggccg cgggcgccat ccacaacgat ttcgaacgcg gattcatcaa
    45361 ggccgaagtc atgtcctttg acgatttcat ggcgcacggc ggttcgcacg cgcgggtgaa
    45421 atccgccggc cgcctgcgcc tggagggcaa ggagtacgtc gttgccgacg gcgacatcat
    45481 caccttccgc ttcaacgtct gacgggtccc gggtctcagg cccggaagcc gtcttcggtc
    45541 attccgcttt tcgtctttac cgctttccgt tccaggactg gttcggctcc gcgtcgcagt
    45601 agccccactg accgctgaaa cggctgccgt caaagacaaa ccggagcttt ccccagtaat
    45661 agcggccgtt cttcgcatag ggacatttgg tgccggacat gtcttcgatc cagaagccgg
    45721 acatcgtgtt tcccgacaac gtgccgataa tttctccatg ctcctgcggc aggtaggcgc
    45781 cggagacctg gtttcccgtc tgccacaact ggatgtctcc gttgtcggtt ttccaggagc
    45841 cgctgacgga aatttccctg ctgtaacccg ggtggcctgc ctggtccgac gactgaaatt
    45901 caattcgggt aatgtcccgg tagggaatgg tcatatgccg gccgtccttg aaatagatcg
    45961 taagcgcatt ttcggccacg gccgctgacg gagccgccca caacatcagg atcatgccga
    46021 tccaaaatat ttttctcatc gctgacctcc ttgaaatgat ttctgtttgt taaaaaattg
    46081 ttcattgcgc agcgtgcgac agaaactttg cgatgttccg tcatcgcgtc ctcgcggctg
    46141 cgtcttccag cgctttcagg taaagctccg tttccgaagc cgatcgattg cccagcggct
    46201 gattcaggcc ggttccttcc gaggaaacgt tcacaaacca gttgttgtcg ttgatccgct
    46261 ggaaacggat cgtgatcaac gtgttgccgt gaaaaaaaga attccgatgg actttgatcg
    46321 cgccttcctc atcgtccttt tcgcataggt cgccggggaa tgtttccctt gccgtttcaa
    46381 aagccagata cgtcgcctgc cagcgcgttc ccgtgaggat caccggacgc agcgccgagg
    46441 acggcgacac cgatgtctgg gcggcgctgc acgctgaaaa gagccagatc agcgcaagga
    46501 aagataaaag tactcctgag cggcgcaaag agcctgtgac catcaggttt tcaggggatc
    46561 gaaaaagttt tctcctccgc agagagccgg ttaaacgcaa tgaacatcag ctccttatct
    46621 ttggtttcca gaatgaatat cgtttgcctc tttttttccg gtcaagccgt tattgcgacc
    46681 attctttttc caatcgccca gaacgaatgc ggctgacggt ttttctagca taagtcgatt
    46741 acaaaagacg agcagtattt gatgggttcc atccgaaaaa acagagttgg gcggggattc
    46801 gatcgggcgg ggaataaaaa acgagaaccg cgaacgccgt cttgtctttt tctggagtca
    46861 ttcgttttac acttggtcta ttgcgggtgg ccatcaccac gaagaccctg tggtttcttt
    46921 ttacagttaa catccagtag aatggactca atacgcgcgt cgcagtttct cgttgactat
    46981 aatctactgc tgtctgtgct taaagtcaag cctggagcgc ttacagccgg aggatggaaa
    47041 aggaaacgcc gggcaggggg atgatgccgg ataaattact gaatttatga ataaaaatgg
    47101 cggagagagc gggatttgaa cccgcggtac acctttaaag ggcgtacaat cgcttagcag
    47161 gcgatcgctt tcggccactc agccatctct ccgctgattt tttcaggcct gtatgtttaa
    47221 aacagtacgt ctcaatctgc aagaagttct gtatgttctg gcggagggag caggattcga
    47281 acccgcgaac ctttcggtca acggttttca agaccgccgc catagaccac tcggccatcc
    47341 ctccatcgta atttttcccg gttatgcttc cggccttacg gagtgatccg ctccaggcct
    47401 ctcatataag gacgcagcgc ctccgggatg acgacgcttc cgtcggcctg ctgatagttt
    47461 tccagaaccg cgaccaccgt gcggcccaca gccagtccgg agccgttcag ggtatgcacg
    47521 aattccacct tgccgctgtc tttccggcgg aagcggattg ccgcccgcct ggcctggaaa
    47581 tcctcaaagt tgctgcacga ggaaatttcc cggtacgtgt cctgtccggg catccaggct
    47641 tccacgtcgt acgttttggc tgaagaaaag cccagatcgg cggtgcacag gcaaacagtc
    47701 cggaaaggga tgcccagcct tttgaggact tcttccgcgt tggcggtcag cttttccagc
    47761 tcatcgtaag acgtctccgg tctggagaat ttgaccattt ccaccttgtt gaactggtgc
    47821 tggcggatca gcccccgcgt gtccttcccg taggaaccgg cttccgcgcg gaaacaggcc
    47881 gaatacgcca ccagataaac cggcagtttt tcctcttcca ggatttcatc ccggtaaacg
    47941 ttcgtgaccg gaacttccgc cgtcggaatc aggtaatagt ccatgccttc aattttaaaa
    48001 agatcttcct tgaacttggg aagctggccg gtcccggtca tactctcggc gttgaccatg
    48061 aagggcgtaa acacctccgt gtagccgtgg tttgccgtat gcagatccag catgaagttg
    48121 gtgatcgccc gctccagctg ggcgcccgcc ccgcgataaa gcgtgaatct tgccccggtg
    48181 attttcgcgc cgcgcgcgaa gtccagaatg ttcaggttct cacccagttc aaagtgctgc
    48241 ttcggcgcaa aacagaagga gggtttttcg ccccaggtcc gcacgaccgt gttgtcttcg
    48301 gatccgccgc cctgcggcac ggattcgtgc tgaatgttcg gcacgatcat gacaaaggcg
    48361 ttgagctcct cttccgtgac gcgcagggtt tcgtcgtact ccttgatttt ggccgatacg
    48421 tcgcccattt tggcgatcag ctcggacgcg tcctcttttt tctttttcag ctcgccgacc
    48481 tgcttggaca cgctgttgcg ttcgttgcgc agggtttcca ccgcctgcag gatgtcccgt
    48541 ctttttgcat ccaggccgac aaagcggtca aagtcaatgt tctgcccccg ttcgctcatt
    48601 ttccggcgaa cggcttccag gttctgtctc aagtacttaa tatctaacat agtttacctt
    48661 cagatctccg gctgacgaat cccgccgaag acgggtttcc ctatcacttt gaactattga
    48721 agtcaatggt tttatcgggg ggatcaatgg ccggaggagg tcagcctttt ttgagctatc
    48781 ttcaaaaagg cgcgcatttc cgggcttttg agaaggtggg cgcaggtgaa atacgcgcat
    48841 gccccggccc caatggcaaa ggagagatag atcagccgat cccgaaagcc tgaggacgtg
    48901 ttccagggca ttccatattc cacaagcccg atggcggcca gcatcgcggc ggaggaagcg
    48961 gcgatcttga aaacggaggt gtaaaacgcg cggtcgagat acggtccgat tttgtttctc
    49021 agcacaaacg ccaggacgcc gacattgacg gcggcggaga tggagccggc cagcgcgatg
    49081 ccgttgtgcc tcagcggata catcagcgtc aggctgaaga ccacgttgac gaccagcgta
    49141 atgatgcccg ccttcagagg ccatttggaa tccttcaggg aataaaaagc ggaaacgaaa
    49201 acacgcagaa ccgaataggc ccagagcccc agcgcatagc agaacagcgc ctggccggta
    49261 tagacggccg cctgggcgtc aaacgcgccg cgctgaaaaa gcacggagat aatgggcaga
    49321 tggagcgcca tcagggcaac catcgatggg atggtcagaa aaagcatcag acgcagagaa
    49381 aaggaaatgc cggatttcag ctcttcggtt ttccctgctg cggcatgcgc ggaaaaactg
    49441 ggcagcgccg ccgttccgat ggcaatggcg aaaatcccca gcgggaactc catgatccgg
    49501 tcggcgtaaa acagatacgt gaccgaaccg cccggcagca gggaggccag caccgttccc
    49561 acaaagacat tgatcgtgcc cacgcccgcg ccgacgatgg ccggaagcat cagggcgccg
    49621 atttgccgga tgcccgggtg gttgaggttg aaccggaaat gaaacgacac gccgaatttt
    49681 ttgagggcgg gccgctgcat ggccagctgc aggacgccgc ccgccagaac gcccgcggcc
    49741 agcgccgtga tcggctgatc aaacgcgtca tgcagaacaa gggcggccgt gatcatggcg
    49801 atgttgagca tgaccgggga cagggccggt gaagtgaaat gccggaaaga attcaaaatc
    49861 cccatgcaca gggcgaccag cgcgataaaa aagatgtagg ggaacatcag gcggttcaga
    49921 aaaaccgcga gctcgtattg ctcgggattg gccatgaagc ccggggcaat cagtccgaca
    49981 atcacgggcg agagcgctat gcccagcgcg gagaccgcgg ccaggatgac ggaaaggatc
    50041 gtaaaggcgt tgtcggccag ttccagcgct tccggcttgg tttttttctg caggtactcg
    50101 gtaaacaccg gaacaaagga aaccgtcagc gagccttcgc ccagcaggcg ccggagcata
    50161 ttgggaatgc gaaacgccac ccagaaggcg tccgtcgtcc agctcgctcc gaaaaaggcg
    50221 gcgatgacca tgtcccggat gacgccgaga acacggctga ccattgtcgc ggcgcccacg
    50281 attccggcgg ctttggcgac ttttgcattt tctgattttt ctgtcggatc gtccatttct
    50341 tcaagccggt ttgcgcgttg cgggtgaatt catgattcag aatggaggtc cagctggcgg
    50401 agcttttgct ccagttccat ccggctgaca tcgttgatca ggatcgtctt tttgcgccct
    50461 cgggcccccg cggaaatctt catccggctt ttcttaagcc ccagtttttt ggccagcagt
    50521 tcgatgcagg cgtcgttggc cgccccttcg accggcggcg cggtgacctt cactttcaga
    50581 aggccttcgg aaatgccggc gatttgcgcc cgcgatgcgt gcggattgac ggaaatatcg
    50641 aaggataagc cgcttttggt ttcccggatg aggagcgtgt cggtcaaagc gttcgccttt
    50701 cttaaaagcc gcgcgccgcc tggatcatgg tctggaccag gaagtactgc agaaagatga
    50761 tgatcagcaa aacgatcagc ggcgaaaaat cgatcggcag gctgtgccgg ggaagcattc
    50821 gccgaatcgg ccccagcacc ggttcggtca cgcggtaaag gaaaacaacg attttattgt
    50881 agggatcggg gttcacccag gaaaccagcg cgcgggcgat gatgatccac agatagaccg
    50941 tcagaacgat gtcgatgact tttgccagcg cgacgatgaa attgctcata ccaatcgaga
    51001 gcatgacccc tccgcctttt gtttttttat tgtctcgtaa aaagttaagg aatcgtcatg
    51061 ccggcggata ccctgaaggg cacaagcgcc ggtatccaga gcaaattaaa atactggatt
    51121 ccgggtcaag cccggaatga caaaaaggag cttcttaact tttgtcgagt tcattatttt
    51181 atttctcaat cgtctgcggc agagccgcca cgaaattctg taaggcgtca ttgaacgcct
    51241 ccggtttttc cagcatggcc atgtgtcccg ctccttcgat caggacgcat tttgccccgg
    51301 taatgccccc ggcaatcttt tcggaagaag cgggcggcgt cattttgtct tccatgccgc
    51361 aaagcgccag caccggcacg cggatgtttt gcagttcttc cgtcagatca aatttgccgc
    51421 acgccagcat gtcgcccgcc accacatcga cgctcgcctg gcccaggctt tcgcgcagcg
    51481 gcccgaagag cttcgggcgg ttctctttgg cgatggagaa tttgcacatc aaatccagga
    51541 tcaaatccgg ctgttttgca aacccgtcca gaatgtcggg attgaccggc atggtcacgc
    51601 cgccgcctgc ggccacgacg ccgcttacct cctgcggata ttgcgcggca aagccaagcg
    51661 cgatggccgc gcccagcgaa tggccgacca gaaccgggcg cgcaagcttc agaacatcaa
    51721 ggatttcttt taatcgaagg acataggcgg aaatatcgcg ctctccgctt ccctccgatt
    51781 gcccgtgccc cggaagatcg acagccgcga tgttgaacga cttgtgcaga accgaatact
    51841 gttcgaccca ggcgccggaa ttgccgccgg agccgtgaag aaagaccaga ctttgcgcgt
    51901 gggcgccgaa atcaccgggg ttgacccagc aggcgatacg gttttctctt accttgaaat
    51961 atttcagcat gagtttttat cctcccgagc ccgtgactac cacaaaagcg ggattttata
    52021 caatcttcaa tattgtgttg acaggatgcc ggcaaaaagg gcatgtattt gccatccgga
    52081 ggacataact atgtttacag gcaagaaagt tgcgattatc ggagggggaa aaatgggcgg
    52141 cattctggcg caggggatga tttcccggaa gctcctttcc ccgcagaata tcaccgtaac
    52201 ggacatcgat ccgcagaggc tggaggagct gcgttcttct ctgaaactgc gggttaccgg
    52261 cgacaacgct gaggcggtga aaggggccga tattgttgtt ctggcggtca agccgcagaa
    52321 ttttccggaa acgctcaaag ggattcggaa agcttcgaat tcctcgaagc tgttcatttc
    52381 catcgctgcg ggtgtgacga cggattccat cgaaaaactg ctgacccgga aaccgcgcgt
    52441 tttgcgcgtg atgcccaatg tcgccgcgct ggcgggcgaa ggcgccgcgg ctgtggcgag
    52501 ggggcatttt gcccgcaaag acgatgcgca gtacgcgctg gcgatgctgg gcgccgtcgg
    52561 tctggccgtg gaagtggagg agaaactcat ggatgccgtg acgggtctca gcggcagcgg
    52621 cccggcctac tgctttgtga tcatcgaggc cctggcggat gccggcgttc aactgggtct
    52681 tggccgggac ctggccgaaa agcttgccgc gcagacgatg ctgggcgcgg ctcgcctgtg
    52741 ccttcaggag aatcagcacc ccgcccggtt gaaaaacatg gtgacctctc ccggcggaac
    52801 caccgccgcg gggctgcggg tgctggaaga ggggaaaata cgcgcgacgc tgacgggcgc
    52861 cgtggaggcg gccgcaaaac gcgcaaaaga actggccggt tcataaccaa cggttgaatt
    52921 ccgctgaagc gggacgagcg ttcattctcc gcagcttgct gcgatatcat gacgttcatt
    52981 tcatacctcg acagcttgct gcgaggtggt tgattaaaaa taaagaagca gcgaggggaa
    53041 aaatggagat caagggaaaa accatcattg tgacgggggc ggcctccggc atcggccggg
    53101 gcctgtgtca gcggtttgca aaagaaggcg cgaaggccat cgtcgcggcc gacatcaatg
    53161 aagcgggcgc gaaggccgtc gccgcggaag tggggggcga ggcgtttgtc tgcgacgtgc
    53221 gcaaagaagc ggatgtcgcc ggtctggtga aattcacgga agacaagttt ggcggcgttg
    53281 accttttttg ctccaatgcc ggcatcatcg ccatcggcgg ctacgaagtg gataacgaaa
    53341 cctggcagcg catctgggag attaatgttc tcgcgcatgt cttcgtggcg cgggcggtga
    53401 ttcccggaat gatccggcgc ggcggcggcg cgatcatgat tacggcttcc gcggccgggc
    53461 tgctcagcca gatcggatct ctgccttact ccgtcaccaa gcacgcggcg gtgggcatgg
    53521 cggaaaatct ggcgatcacc tacggcggcc agggcattca ggtgtttgcg ctttgtccgc
    53581 aggcggtcga gtcggaaatg acgcgtgggc atgaaggcgg cggcgtggcg ggggtggacg
    53641 gcctgatgaa gccggagcag ctcgcggacg ccgtcatgga atcgtttgcc gccgatgaat
    53701 ttctgattct gccgcacagg gaagtcaaaa cctacatgca gcgaaaggcg gcggattaca
    53761 accggtggat tcaggggatg cgcaggctgc aggagagatt cttcaccggc gcgccgttaa
    53821 aaaagtaaga agcggtctga agcggtttct ttggtttctt gcatccggta agggggaagc
    53881 ccatgggtgg cgaatcctgc gtgtcggtgg acaaccttca ggatctggaa caggcgctcc
    53941 tggtggccga cagggtgatt gtcctcgtgc acgccacctg gtgtcccttc tgccggaagg
    54001 ttctgccggt gtttgaaaaa ctggcgcgga aggaaaacca acacctgctc ctggtggccg
    54061 acgatgagga acgcattgcg gatgtgtacg acgtcgatat tttcccgacg ttgatcctgt
    54121 ttgagaaggg ccgcgtcgtc aaacgcctcg acggcaagcc gggggtgggc ctgagcgaaa
    54181 aacagattgc cgacttcata aagtcctgtc ggccggctga gcccggttaa ccttcggagc
    54241 cccatacccc tggatatttt cgttcgatcc tgcggcggct gtaattgtca gctggatcga
    54301 tccaaactgg tgagcgacgt tcaacgcttg cttggccctg cgttccgcct gacgacggat
    54361 cccgcaggcg cgcctcacaa aacaggactc ctggtgtgcg gctgtccgac ggcctgcgcc
    54421 gaaaatcccg aaaacagcaa tcgggcgcgg cgctgggtgg tcgttgccgg caaaaccgtg
    54481 agtgcccgcg agctgacgga ggaccgtctg gcggaagcgg tcgcggagga aatcaaaaag
    54541 atcatttttt ctgagtaaaa atagatagat aggtttatta tctgatcata gactacaaat
    54601 atcctttaaa aaaaatagca tgactatgat aaaaaataat acttgacagt gggactatga
    54661 agatgtagct taaatcaaac ttaaaaaagg atattggaat ggccgatcgc gttcgagcaa
    54721 aaaccagaaa agaccgcatt atggatgcgg ctttgcgcat ttttgccgaa aagagttttc
    54781 aggaggcaac gatatccgaa atcagcaagg aggccggtgt ttccgaggcc acgatttatg
    54841 agtatttcgg caccaaggaa gatctgctct ttgccattcc cgaaaaaata tccaatgaca
    54901 cgtatgaaga atccaaagcc gtccttcctt acatcaagga tgtggaaggc cggatgcgcg
    54961 ccatcatgct gagctatatt cagctctacg aaagcaatcc gcactattcc gcgctggtgc
    55021 tgctgcagtt gatgtccaac aaacgtttcc ggcagaccgc cgcgcatgcg gccatccggc
    55081 gctccgcgca cagtctgctc gactgcatcc gcgaaggcat cgccaacggc acgttccgtc
    55141 ctgatgccga cgcctacctg atccgctcga tgctgatggg caccatcgag catctgttta
    55201 ttcactggca tatgcaggga atgccccggc gcgacaaaac catgatggag atgctggatc
    55261 cgtttctgga aatcgttttg agcggcatcc gggtgagaaa ggagcagcag gggcttacgc
    55321 tgcatttgaa tctggaggat gcgcgcgccc tgggacagat tctgcaaaat gatccggcgc
    55381 tgcaatcccc ggcccagagc gcggacgctt cctgtgcaac gaaagaaaaa aattaaacaa
    55441 cgcatataaa agaaaggagt acctctctat gagaaccaag caagatttta tcaacgccct
    55501 gtcgaaaatg aaacgcaaca tctactatga cgggcagctg atcgaccgga ccgacgagct
    55561 gcaaagcgac tgcatcaacg tgatgggcac aacgtatgat gaggcggcca agccggagaa
    55621 cgaagtgctg atgacggcca cgtctcacct caccgggcag aagatcaacc gcttcacgca
    55681 cattcaccag aacacgaccg acctgcaccg caagcaggac atgacccgca tgctctgcca
    55741 gaaagtcggc ggctgcattc agcggtgcat gggcatcgac ggcaccaacg ccatctacaa
    55801 cgtatcgttt gaagcggaca aggtgaagcc gggcgagacg aactaccacg agaatttcaa
    55861 gaagtggctg acccgctttc agaaggaaga catgattgcc gcctgcgccc agacggacac
    55921 caagggcgac cgtatgctgc gtccggccga acagccagat ccgagcgctt acgtgcgcat
    55981 caaagaacgg accaaggacg gtatcattgt ggaaggctgc aaactgcaca tctcggaagc
    56041 ctccgtttcc gatgaagtgc tggtgattcc cacccgcgcg ctgcgcaagg aggacaagga
    56101 ctacgccgtg gcgtttgccg tgcccgcgga ctacgacggc atgaagcagg tggtgaccat
    56161 tcacaattac cgccagcggg agcacttcaa acgcggattc acgcccgggc tgaccgattc
    56221 gtatctgatt ttcgacaact gcttcgtacc gtgggaaagg gtcttcctgg ccggcgagca
    56281 cctctatggc ggcgttctgg cgctgctctt cgcgctgttc catcgtcatt cctactccgg
    56341 ctgcaagccc gccatcggcg acgtcattct gggcaccgcg gcgctggccg ccgaatgcaa
    56401 caacgtccac aaggagtccc atgtccgcga gaagctcgcc gaaattatca tgacgatcga
    56461 gctgggctat gcggcgggct acacggcttc ggatctgggc aaaccggaag tctatattcc
    56521 cggcgtcggc ttcatgcctt acggcccggg ctcctacatt ccgaattcca tctactgcaa
    56581 tgtgggccgc tgtctgtcgg gcgaggcggt ctggcgcgag gcggagattc tttgcgacat
    56641 cgcgggcggc gtcgtggcga cgttcccgca cgagaaggat ttcgtcaatc cggatgtcgg
    56701 tccggcgctc ctcaaataca ccaagcgcaa tccgaagatg tccgccgagg accaggcgca
    56761 gttctggcgc tacctgggcg attcgatgtg ttcggccacg ggcggcatcg ccaacgtcgg
    56821 caactaccac ggcggcggtt ctccgatcat ggagcagatt gccatcacga cgcagtacga
    56881 catcgaatcc cgcaagaagc tggtgaaatt catcgccggc atgagcggcg gcgatcgcga
    56941 agccctggcg ccgaaagtca agaaataaaa tggattcacc cagggggcct gacggctcag
    57001 gccccctttt atcatcattt aaaaagaaag gacggcgcca tgagaacaaa gcaggattat
    57061 attaaaggcc tttccaaaat gaagaggaac atttattttg acggccagct gatcgatcgc
    57121 gacgatgaat tgcagatgga ttgcctcaat gtcatcggga ccacctatga cgaagcggcc
    57181 aagccggaga acgcggattt gcttaccgcc acgtcccacc tcaccgggca gaagatcaac
    57241 cgcttcacgc acattcacca gaacacgacc gacctgcacc gcaagcagga catgacccgc
    57301 atgctctgcc agaaagtcgg cggctgcatt cagcggtgca tgggcatcga cggcaccaac
    57361 gccatctaca acgtatcgtt tgaagcggac aaggtgaagc cgggcgagac gaactaccac
    57421 gagaacttca agaaatggct gacccggttt cagaccgaag acctggtggg ctgctgcgcc
    57481 cagacggaca ccaagggcga ccgtatgctg cgtccggccg accagcccaa ccccggcgcc
    57541 tatgtgcgca tcaaggaacg gctgaaagac ggcatcgtcg tggagggctg caagctgcac
    57601 atttcggaag cctctgtggc ggacgaagtg ctggttctgc ccacccgcgc gctgcgcaag
    57661 gaagacgcgg actacgcggt cggctttgcc gtgccgggcg actgggatgg tttgaagcag
    57721 gtcgtcacga ttcataacct tcgcaatcgc gaacattaca aacgcggttt cgtgccgggc
    57781 gcgaccgatt cctacatgat ttttgaagac tgcttcgtgc cgtgggaaag agtcttcctg
    57841 gccggcgaat atcagcacgg cggcgctctg gcgctgctct tcgcgctgtt ccatcgtcat
    57901 tcctactccg gctgcaagcc cgccatcggc gacgtcattc tgggcaccgc ggcgctggcc
    57961 gccgaatgca acaacgtcca caaggagtcc catgtccgcg agaagctcgc cgaaattatc
    58021 atgacgatcg agctgggcta tgcggcgggc tacacggctt cggatctggg caaaccggaa
    58081 gtctatattc ccggcgtcgg cttcatgcct tacggcccgg gctcctacat tccgaattcc
    58141 atctactgca atgtgggccg ctgtctgtcg ggcgaggcgg tctggcgcga ggcggagatt
    58201 ctttgcgaca tcgcgggcgg cgtcgtggcg acgttcccgc acgagaagga tttcgtcaat
    58261 ccggatgtcg gtccggcgct cctcaaatac accaagcgca atccgaagat gtccgccgag
    58321 gaccaggcgc agttctggcg ctacctgggc gattcgatgt gttcggccac gggcggcatc
    58381 gccaacgtcg gcaactacca cggcggcggt tctccgatca tggagcagat tgccatcacg
    58441 acgcagtacg acatcgaatc ccgcaagaag ctggtgaaat tcatcgccgg catgagcggc
    58501 ggtgaccgtg aagctctggc gcccaaaccg gccaagaaat agtctgttta cgcttcggat
    58561 catcctgcgg gggaacggct tgcctgacct gaccgcctga tagcctaaag gtcacgcgag
    58621 gcggcgcgag gttacaagac cgttccccgc aacggatact ccatattctc ttgataaagg
    58681 ggggtgtaca ttcccattca atttattttc tgctgcatta aataacagta aaggagtgct
    58741 tgctatgttt gatctttcca ggttttcttt agaaggtaaa gtggcgattg ttaccggcgg
    58801 cagccgcggc atcggccagg ggatcgctta cggttttgcc aaagccggcg cgaaagtggc
    58861 catcaccagc cgcaaggcgc aggatttgga agccaccgcg tgtgaaatga cgaatatcgg
    58921 ctgtgaagtt ctggtggtgc ccgcccattt gggcaagatg gaggaaattc agcgcgtggt
    58981 ggatacggtt atgcaaaagt tcggccggat tgatattctg gtcaacaatg ccggcgcgag
    59041 tcccgccatg gggtcggtgc tggattccga cgaacgtctc tgggatacca tcatgaacct
    59101 gaatcttaaa gccgtttatt tcatgagcca ggcttgtgcg aaaatcatga aaaagcaggg
    59161 cggcggcaag atcatcaacg tagcctccat tgacggcgtc aatccggagc cgtttgtcag
    59221 cgtgtattcc atttccaagg ccggcgtccg ccagatcacc cgcgcgtttg ccatggaact
    59281 ggcccgcgac aatatcatgg tcaacgcgat tctgcccggt ccgatcagca ccaagatgat
    59341 gaattcgcac tggcaacacc ttccgccgga agaagcggcc cggcagaaag aggagttgtg
    59401 caagcatctg cccacctgcc ggatcggcga accggatgag atcgccggag cggcgattta
    59461 tctggcctcc gacgcatcga gctataccac cggcgcggag atcatcatcg acggcggtct
    59521 gcttttgggc ccgcatctgt aaaaaaggca acagccgctt ttcaggcggc cggtattttc
    59581 cgatgcgccg ccgtccaaac gcgtgaaggc ggcgcatccc tacttctgca aaggagatcg
    59641 tttatgaaaa cccttgagca tttgttgtcg cccatcaaaa tccgttccct ggaaattccg
    59701 aaccgtctgg tgatgtcccc catgggaacg gctctcggca atcccgacaa taccgtcagc
    59761 gaggcaaacc tggcctatat ccggcgcagg gccgagggcg gcgcagggct catcatcacg
    59821 gagatcacgg aggtgcaccc gctgggttcc gcctccccgc gatgcatcgg cgtctgggac
    59881 gacaagttca ttcccggcct gagcaagctt gccgatgtgg tgcatgccca gggaagcaaa
    59941 atcgccatgc agcttcacca tacaggccgc gaaaattacc tcctgcagaa gaaaaacaag
    60001 gctgtcgggc cttcggccat ccccagttat gtctttggtt ttctcggaac gccgcgcgag
    60061 atgacgcttg ccgaagtcga ggaaacgatt gccgctttcg gcagcgcggc cgtgcgggcg
    60121 cgcaaggcgg gatttgacgc cgtcgagctt cacggagcgc acggctatct cctcatgcag
    60181 tttctttcgg ccctcagcaa tcagcgcaag gatcggtacg gcggcgattt tcgtggacgg
    60241 gcccgcttta tgatcgaatg cctcagggaa gttcgccgtc aggtcggcac ggatttcccc
    60301 gtttccatcc ggatttccgg cgaagagtgc atcaaaaacg gctacacgat ctccgacatg
    60361 gagaccatcc tccccgacct ggtgaacgcc ggcgcggaca tcatcaatgt ttccttcggc
    60421 acgcacggca gcccggccgt gaacatcgat accccgaatc caagcgcgcc ggttgaatac
    60481 ccccccggct tcaagtccca cctggcgcgc agggtgaaag aggccgcggg cgttccggtg
    60541 atttccgtcg ggcgctacac ggatccttac tttatggatg aagtcatcgc ccgaggcgat
    60601 gcggatatga ttgccgtggc gcgccagcat ctggccgatc cggattttct caaaaacgcg
    60661 ctggccggtc atcccgagga cacgctggag tgtctggcct gcaatcaggg gtgcattgag
    60721 cgcctctcgc tggaggcgct gccgattcgc tgcgccatca atccgcaaac cggtcaggaa
    60781 ctgatgtatc cggcgggtcc ggcggctgtt ccccgcaagg tctgggtggt cggcggcggg
    60841 ccggccggac tgaccgccgc ttacgaggcc gcgcgcctcg gccatcaggt aaccctcttt
    60901 gagcaggaaa gccagacggg cggaaatgta cgctatgccg ccaaagcgcc ccacaaagag
    60961 gtttacggcc gctacattca gaccctcacg gcaaaatgca aaaagaaagg cgtggagatc
    61021 aaaaccggcg ccgaagtgac cgaggcgatg attgaagcgg gcaaacccga tgccgtcatt
    61081 ctggcgatcg gcgccggcaa agccgcctgt ccggccgaag gcatcgatgc gtccgtggtt
    61141 tgcgacgcct ggcagattct ggatgggacc gttgcgccga aagagccggt ggtggtcatc
    61201 ggcggcgggc tggtgggaat ggaaacagcc gattttctgc tggaaaaggg cgtccggaat
    61261 atcaccatcg tggagatgct gcccgcgtcc cccgttctgg cgctggccgc gcacggctac
    61321 atgctgcaca agcgccttcg ggcggccggg attgccctga tgctgggaac ccgggtgaaa
    61381 aagatagaag aaaacgcggt gatcgtcgag gtcaagggag aggaaaagag attggagccg
    61441 gtttcccagg tgattgtggc catcggcgtt acgccccgca cggcgctgaa ggacatgctg
    61501 gcgaaaaaaa gcatccgtca ttttgtcgtg ggcgacgcct ccgctccgcg aaggatcatc
    61561 gaagcgacga cggagggggc gaaagccgcc tgggaagtat aaaaaaggct gaagacggaa
    61621 gaccgaaggc tgaaggaaaa gataaaaaaa gtgggtgcga agcgccccgc aaaatgatcc
    61681 aatagtcttc agtctcatcc cctcaataaa aggaggccga aatggatttg cgtgagcgat
    61741 acattgaaac caacggaata aaactgcacg ttatggaatc cgggccggcc gacggcccga
    61801 tggttctttt tctccatggc ttcccggaat tctggtatgc ctggcgcaaa cagatcgggt
    61861 attttgcaga ccggggctat ctggtggttg cgcccgatca gcgcggctat aatgtaagcg
    61921 acaagcctgc aggcgttgcg gcctataata ttgatgagct ggccaaggac atcattggac
    61981 tcgtcgacgc ctatggacgg aaacagattc tgctggtcgg ccacgactgg ggggcgtccg
    62041 tgtcgtggtg ggtggcgctg aaatatcccg accgcgtccg aaagctcgcc attctgaatg
    62101 ttccccatcc caaggtcatg gccagaaatc tctttaccaa tcagcggcag atggaaaaga
    62161 gctggtacat cttttatttt caactgcccg gcgcggtgga aaaattcggc tcggcccata
    62221 attatgcctg gcccgtgcac ctgcttgcga ccacgagccg tcccggcgcc tttacgcctg
    62281 aagaactgga agaataccgc aaggcgttcg cgcagccggg cgctttttcc gcgatggtca
    62341 actggtaccg cgccatgatt cagacgcagc ccgaaccgct gcaaagcttt gacgtggtca
    62401 tgcccatgat tttgatgtgg ggagaagacg atgtcgccat gcttgcggag atggccgatc
    62461 agagcatggc ctattgcaag cagggcaggc tcattaaatt tcccggcgtt tcgcactgga
    62521 tccagcatga ggaagcggac cgggtgaatg cgctgatcga ggaattattc atcgcgtgag
    62581 caagccccgg cgaaggcctg cgcggccgca tccggggagg gccgatcgcg aaaaatcata
    62641 aggaggaaag ttatgaaaaa aggaatcgtt attttgggag caggattaat tctgttgttg
    62701 atgtcgggcg cggcaacagc tcaggtcaag agccccgcca tgatccggac ggcttattcg
    62761 gcgatggacg cgaacggcga tggcgtcgtc accgttacgg agcacgcggc cttctggcag
    62821 ggacggttca aggacatgga tgccgaccgc gacggcaagc tgacggcggc ggaattcaat
    62881 gcggcggaga aacggttttt cggcgacatg gatacggacg atgacggcgc gctgttggcc
    62941 aaggaatata tcgcttactg gtgcggaccc aaggcgcttg aacccgccaa aagcaaggcc
    63001 aagacaaaga ccaagtcgaa gaaaaagctg gacgccgaca aaaacggcaa aatcggcgac
    63061 gatgaatgcg tcgcgttctg ggtggcggag atttttgatg tggacgcaaa ccaagacggc
    63121 aaagtaacct ccgaagaata catggccgcc atggcctccg ctttcaagga aatggacaag
    63181 gaccgcaacg gatttatctc catcgaagag cacgcgtact tcatagcgaa aaaagccggc
    63241 gccgcgaaga aataagccgg ataatttaca acgggtgttg tattcacttg acgcggggat
    63301 ttgggtgtga taacgcccca ttcatcaatt ccaaagagcc gtttggcggc gggaatatca
    63361 ggtgttgtgc aaaaagacga agcatcgttt tttttaaatt gaacaatgga caaggagggt
    63421 ttatgagcag agaagtagta tttgtggatg gcatgagaac agcctttggc accttaggga
    63481 aaaccctgcg caatatcctg ggtgaagatc tggccggcat gaccctcaag gggctggtcg
    63541 aaaggtcaaa aattgttgaa aaaggcggcg tggtggacgg cgttttcatg ggttcggcca
    63601 ttggacccaa caccgccatc aacggcgcgc gctggcctac tctggcttcc ggcttgcccg
    63661 agagcaccgt ggcttcttat attgaaatgc agtgcggttc ggcgattgat tcgatcaatc
    63721 acgccgcctg gaaaattctg gccggtcagt ccgacatcat gatcgccggc ggttatgaat
    63781 cctacagcca ggcgccgtgc cgtttttcca tgtcgcagga accctttaaa atgattcccc
    63841 cgcttccgat cgctcccgcc ctttccccga ccagagatcc ggccaccatg aacatgggtc
    63901 tcaccgctga agctctgcag aagatgtaca atatttcccg tgaagcacag gatgaatttt
    63961 cttaccgcag ccagatgaga atgaaagcgg cccaggaatc cggtttcctg gcccaggaca
    64021 tcatgccggt catggttccc cagggaaaga aaaaaccgcc gctggaattc agcgtggacg
    64081 aatttccccg tcccgatacg acgatggaag gtctggccaa actgccgccc gcttttatta
    64141 aagacggcac cgtcactgcc ggcaacgcct cgggccgcaa cgacggagcg gcattcgtgc
    64201 tgatgatgac caaagagaaa gccctgcaac tgggatacaa gccctgggcc aagtggctgt
    64261 ccggcggcga ctacggcgtc gatcccaaga tcatggggat tgcgcccgct tatgcggttc
    64321 cgatggcgct caaacgcgca ggcatcaaga tgagccagtt ggacgtgatc gagtgcaacg
    64381 aagcttttgc cgtgcagaat ctggcggtgg ccgcggaact ggaaaaacag accggtgaaa
    64441 aagtggattg gaacaaatgg aacccgaacg gcggcgcgat ctccatcggc catcccaacg
    64501 gcgcttccgg cgcgcgcatc tgtatcttca ccatgcgcga attgatgaag aagggcggca
    64561 agtacggttg cttcagctcc tgctgcggcg gcggtttggg cgtggcctgc gtggtagaga
    64621 atctgacgtt ctaaacaagg ttttccttta gaaaaacata ttacggggta aacggaatct
    64681 ccggagctga aaggctccgg agatttttca cgaa
//
LOCUS       DAOSRM010000004        49364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_797_length_49364_cov_109.275731, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000004 DAOSRM010000000
VERSION     DAOSRM010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 49364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49364
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_797_length_49364_cov_109.275731"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..820
                     /locus_tag="PLA58_01065"
     CDS             284..820
                     /locus_tag="PLA58_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61413.1"
                     /translation="MIRYFNATHIPVAARAFSDAEKLVSRHFRLSENDLRKNKYDVKT
                     LAFLEEHEVKDGAFAHLCKYSYEKPSDGSPEGKEGFDFYRVCLQDNIILDAVERANTF
                     IKFSPLMLYIAAHELIHVLRFGSGAIDFNASLQEKEQEEKIVHNLTRSALQPVKQNDL
                     EIVLDCFSSEFSISDLYN"
     gene            839..1123
                     /locus_tag="PLA58_01070"
     CDS             839..1123
                     /locus_tag="PLA58_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOR61414.1"
                     /translation="MPIYEYQCRKCGKQFEAFQGITEPDLKTCKFCKGKVHKMMSLSS
                     FSLKGSGWYATDYAGKKPQTVNPKSTETATAESSSASSDASATVKGVSED"
     gene            complement(1420..2454)
                     /locus_tag="PLA58_01075"
     CDS             complement(1420..2454)
                     /locus_tag="PLA58_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61415.1"
                     /translation="MRDIGFHKETVTGRHLSPFDGFEPKDMTIEERYDETTGVASRIL
                     PYRVRPAQKPDTAVYLEKSPESICPFCPALFDKITPRFTPDIIEGGKFTRGSAFLFPN
                     AFPYDATNTVAIFSHRHFVGLDSLTPDAMRDGFAVCRDYFYRMAELQKGFRYGSINWN
                     YMPPAGGGLIHPHLQTIAGKKPTSFMRRLLASAQNYEAASGGNLWKDLLDREQDAGER
                     FIASTGVVSWLAAFSPKGMAGEVDFYFREKTSFFDLTQANFEELLKGLSRVFLHMYVN
                     NFMSFNLSLYAPMLPEKNFWVQGKIVPRFEINPLGTSDLNYFEKLHDEIICPIVPEQL
                     CKELQAFFQE"
     gene            complement(2451..3515)
                     /locus_tag="PLA58_01080"
     CDS             complement(2451..3515)
                     /locus_tag="PLA58_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOR61416.1"
                     /translation="MHRLPFKSETQQNVKKLLELAQPDDSLLVLMHGSPDPDAIASAM
                     ALREILLRTKGLSKWVFAATEPIRRQQNRELAQAMRLNIRLLNQIDAGACRLVALVDA
                     QPSFFGDSLPAREPDIVMDHHPREGGWQAELADIRPSYGALSTILTEYLLAARVRIPR
                     NLHTALLYGIKTDTDNFDRETLAEDIRAYTYHSRRANLRLIRRIELNQTPRSYLKYFD
                     HAFHSMTHFRGRRVGFLGNVESPDVCVQVADFYLHVIGTSYVVVAGIVNDRLVIIFRG
                     DGYRQDCGAMAQKACGQIGQGGGHRSAARLEIPLDVLKAELQGDLSHESVERFLHQCL
                     RKSASRKEEPPPTENGKEGT"
     gene            complement(3522..4574)
                     /gene="selD"
                     /locus_tag="PLA58_01085"
     CDS             complement(3522..4574)
                     /gene="selD"
                     /locus_tag="PLA58_01085"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4521..4523),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOR61417.1"
                     /translation="MEEKKSVRLSQTVSSAGUACKIGPGDLSDILCDLPLVSDPKLVT
                     GYEHAEDAGVYKISDDLALVQTVDFFTPTVDDPFTFGQIAAANAMNDIYAMGGTPLTA
                     MNIVCFPVKTMDKSVLRDVLRGGLDKMREAGALLVGGHSVEDNEIKYGLSVTGTIHPD
                     RVLFNRGARAGDRLILTKPLGTGIVATAIKAGAVGQALIEQATRCMTTLNKKAAELMI
                     AAGNIHSCTDITGFGFLGHACETIEGSDVGLRIEAARIPILEGVRDLVESGYLPGGLY
                     RNRNYRLHQVEKAPSCPDWIFDVCFDPQTAGGLFFSLPSGKAEPLAEAMRRAGITDAA
                     VVGDVVADHSGKILLE"
     gene            4881..6284
                     /gene="thrC"
                     /locus_tag="PLA58_01090"
     CDS             4881..6284
                     /gene="thrC"
                     /locus_tag="PLA58_01090"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOR61418.1"
                     /translation="MKGIPGMSIRYYSTNRHVKPAAGITPFTGSVSFREALLAGQSPD
                     EGLFVPDAIPQLPMKDIVALRNQPYWQAALLVARAFLSEEFSLEVLETIVKDAYNFPV
                     PLEAVYPRAFILRLDQGPTASFKDFAARMMARLMSHCRPEGERLNILVATSGDTGSAV
                     GEAYKGVDGIDVTILYPADEVSLMQKKQLDTIGGNVRAICVRGKFDDCQNLVKEAFAD
                     KDLARFNLSSANSINFGRILPQIVYYIYAYAQLAGAGEEAVVSVPSGNFGNALGCEYA
                     RRMGLPVAKLIMPTNENDEFPAFLETGTYAKVSPSRACISNAMNVGHPSNLARFFELY
                     GGTVDRTGMVHRYPDLDEMRRRIFSVSVSDGETRQTIKSVYERYRVVLEQHGAVGWAG
                     LTAYQKKTGDTRLAICLETAHPAKFPDEIKDLLGIDPEPPESMKDLARREGSAINLSG
                     DFASFKTYLQAHLKIKK"
     gene            6333..7091
                     /locus_tag="PLA58_01095"
     CDS             6333..7091
                     /locus_tag="PLA58_01095"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HOR61419.1"
                     /translation="MKKPATPPKNRQTTGQDFSPGDLIVETGRFFLGAPYREGTLEGP
                     GREKLIVNLHAFDCTTFVETVLALVRSARFGKLRAPEFRRNLRYIRYRGGKIDGYASR
                     LHYFTDWIIDNQKKKTLKDVTRHLGGARRQKEIHFMTTHRERYAALTHQNQAKRMRQT
                     ERRLSRRSFYMIGKERVSQIQDGIQPGDILAFTTNQDGLDVAHVGLAVRERGRLRLLH
                     ASRREGAVVVSAETLPAYLRRRGNFTGILVARAL"
     gene            7207..7644
                     /locus_tag="PLA58_01100"
     CDS             7207..7644
                     /locus_tag="PLA58_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOR61420.1"
                     /translation="MNYVRDILKTKDGRIQTISPKATVYEALEKMSEKEIGALVVMEN
                     KKVVGIFSERDYARKIILQGKTSKKTLVKEVMSSHLFSVTPDTTVEDAMVLMTGKHIR
                     HLPVFEKSKFVGIISIGDVLKLIISNKDFLINQLSDYIAGKYY"
     gene            7888..9351
                     /locus_tag="PLA58_01105"
     CDS             7888..9351
                     /locus_tag="PLA58_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOR61421.1"
                     /translation="MDMVNAAASVVKNIRIQDILDMAIIAGMIFALLMWFKTRASRFV
                     LIGILLLGGVYLAARFFQLYLTVIVLQGFFAILLFVLVVIFQEDLRGFFERIAMLGQL
                     GKQAPPLSRLQKTANIISETAGNLAKKRIGALLVIQGNDPLDRHLNGGVPLNGAASEP
                     LLESLFDPHSPGHDGAAVLKDDRVAMFGCHLPLSVNTARHGNMGLRHTAALGLTERSD
                     ALCVVVSEERGSISVSEGKTLSVVPGASALHEILEKHYAKYRPAPKKRPLRAWLSENT
                     KEKVIAVGLACLLWAAFGYQRDTVRRDFFVPLAYKNLPAGWHFDAPPVKEAKVTLQGP
                     EQAFRLMNEDSLKLALDVSALAQKNQEFSLNRNMLNTPTGLSIAEISPAKIKVFASGQ
                     VVAHLPVHVATVNRLPDHLVLQKISVKPAALRVLLDSWIKPAGLRLETEPLDLQSVTS
                     SMTVALRVNAVSGASFPDGKSARVQVAVRVKPKEPTP"
     gene            9348..10409
                     /gene="corA"
                     /locus_tag="PLA58_01110"
     CDS             9348..10409
                     /gene="corA"
                     /locus_tag="PLA58_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOR61422.1"
                     /translation="MTLAVKKRSKKAGLSPGTLIHIGHAYEDSSRLRLMRYDEAALVE
                     KENFSPGELIEEKKKPGVLWVHLDGLRDIGRLEEIGRIFGLHPLILEDILNTDQRPKA
                     EDFTDYIFIVLKNIHHPAGQNLHAEQISILLGPNFVLSFQEKAGTLFDPIRERIRTDK
                     GRIRKAGADYLAHHLMDAIVDQYFAVLEDIDDKIELLEEDLVKKTTPDKLQAIHLLKR
                     ELILLRKSLWPLREAIGLLQRADSPLIADSDLIYFKDISDHIIAVIDTVDTFRDMLAG
                     MLDIYLSSASMRMNQVMKVLTIIATIFMPLTFLAGIYGMNFAYMPELGWRWGYFAVLG
                     LMLTIALGMLMLFRRKKWI"
     gene            10489..10971
                     /locus_tag="PLA58_01115"
     CDS             10489..10971
                     /locus_tag="PLA58_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOR61423.1"
                     /translation="MQVFAASDIVEVAIRIEENGINFYKFAEQIARQEDAKKLFSQLA
                     QAEAAHKRTFEKFFADMEKYNPPEQYAGEYSAYLRNYVDNNLIFTKEIMDRQLAKVTD
                     TVGAFEFAMQRELDSVLYYHEIKNMVPAAQHEAIDKIIEEERRHYTMLCDMKKRYENK
                     "
     gene            11046..11804
                     /locus_tag="PLA58_01120"
     CDS             11046..11804
                     /locus_tag="PLA58_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR61424.1"
                     /translation="MKKKTVNKMLRILYQPYKWLIFAPYLAVSTLFFGSLTMVLAVVT
                     NPRITSFICGTIWARLNGYLTPIRVTVTGRNHIVKTQSYVIVANHQSQYDIFVLYGWL
                     GIDFKWVMKQELRKVPGIGIGCEKVGHIFIDRSNHEKALASLAAAKEKIVNGTSVIFF
                     PEGTRSRDGRLGAFKKGAFKMAVDLRLPILPITIVGTRDILPTDSVDLFPGRARMIIH
                     KPIDTAGYTDDNLDELVDRARSVIESGLRETAAG"
     gene            complement(11856..13217)
                     /locus_tag="PLA58_01125"
     CDS             complement(11856..13217)
                     /locus_tag="PLA58_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOR61425.1"
                     /translation="MQKLHILIVEDGRSQREMLRDFLISEGHRVMEAENGEAAVTTVA
                     NHHFDLILLDYKMPGMDGLAVLKEVKRINHEIDVVIITAYGTIETAVEAIKAGAVDYI
                     TKPIELDELLILVDRVAERRGLIRENDLLKQELGKTGVTAEKIIYKSARMVELMNMAS
                     RVAPSRATVLISGESGTGKELLARLIHQLSPRAARPIVVVNCGALQENLLESELFGHE
                     KGAYTGAGSRRIGRFEEADGGTLFLDEIGELSPAVQVKLLRFLQEKEISRLGSNASIS
                     VDVRIISATNRDLEARVREGAFREDLFYRLKVVAMSLPPLRERKEDLPLLLDHYLEKF
                     AAENGKSLRGITAEARDLLLKYDYPGNIRELVNIMERAVVIARNDYITVSDLPFRPGS
                     FSGAPGGSSAGTLRDSVEELEKRLIGEAMAKASDNQTKAAEFLGMSERMLRYKLKKYD
                     LKN"
     gene            complement(13237..15039)
                     /locus_tag="PLA58_01130"
     CDS             complement(13237..15039)
                     /locus_tag="PLA58_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR61426.1"
                     /translation="MLFFQKKKAITQRGAHSYNGNFLQPLAVGIVSAVLVALILVMGY
                     LDIRRNETNLVGFMEDQALTTISVLLRQTEDNLKSIAAAKGKMPSHLKTAKEAESAYS
                     IQLVYDAITKLGQQIDEQWKKKLLSNAYLRKFAADNHFWYAAVLNMRGDVIYQSGPLK
                     TDMLPKSDLTVKGRKATTVELLEKIRIKQDIGYVGMKLKGDSGIAVFCLDRQGRLYWS
                     LKVAVERAMSKMGEGHGIAYLQVFNDQKILLGNIGELPAGLTSEDLNDQEMMRGKKRI
                     LSRKVDRQGIKILDVAAPFVMENPYLPREKIAGIVRIGLDRGSMDKIIAENRQNIFIF
                     MLFVVGIALLSMWLLYHDQNRHLAGIIEMERRLEKAERLSSLGQLAAGVAHEIRNPLN
                     AISMATQRLKRDFIPPDETKAREFKNLSTVIRDEIRRLNGIIEEFLSFSKSRRLELSN
                     FSVTEVLQKIVSLMAEEAASRNITLETQWRLSPAVIPMDINKLQQAFLNFIKNAMESI
                     TNGGKITITVSREGKKHVLVSIADTGCGMTPEEIEKVFSPEYTTKEKGLGLGIPLATE
                     IIRGHGGDVRVISRPGEGTTFEIVLPVEKISDKT"
     gene            complement(15496..16860)
                     /locus_tag="PLA58_01135"
     CDS             complement(15496..16860)
                     /locus_tag="PLA58_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOR61427.1"
                     /translation="MKNLDLFSQGTPDDALDTAPLAERMRPLTLADYIGQPHLVAEGT
                     LLRRSIAEDKLFSMIFWGPPGSGKTTLARILANETKANFVSFSAVLSGVKEIRQVIDD
                     ARDLWEAKKQKTILFVDEIHRFNKAQQDAFLPHVESGLITLIGATTENPSFEVIAPLL
                     SRTRVMLLRQFTDEDLMAILKRALSDPQRGLGKFAIQIAEEALQFIIGISDGDARTAL
                     NNLEAAASMVQSLPGEERTITAKSAEEALLKKALLYDKDGEEHYNLISALHKSMRGSD
                     PDATLYWLGRMLAAGEDPLYILRRMVRFASEDIGNADPHALVLTMAAQQAFHFIGLPE
                     GELAIAQAAVYLAVAPKSNSLYTGYGQTKDLINKTGYLPVPLHIRNAPTKLMKELEYG
                     KDYKYAHDYSDAYVPQEYFPDKLQGKVLYSPTDAGYEKIIKERITEWRRRKTEAKKGS
                     EKKG"
     gene            complement(16857..17114)
                     /locus_tag="PLA58_01140"
     CDS             complement(16857..17114)
                     /locus_tag="PLA58_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61428.1"
                     /translation="MKTPHDHKITRCPKLGDEMTFAYCLQEAGDLPCARIIFCWQAAF
                     DVASLLEENLTQEEQKRFADAQPKEKVVSLIEILEKAKRQT"
     gene            complement(17306..18667)
                     /locus_tag="PLA58_01145"
     CDS             complement(17306..18667)
                     /locus_tag="PLA58_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOR61429.1"
                     /translation="MEQVKTLLEDHEIPRQWYNVLADLPTPMKPPLHPGTGQPVTPQD
                     LAAIFPMNIIEQEASAQRWIDIPDEVLQKYVLWRPSPLYRARNFEKLLGCPVQIYYKN
                     EGVSPAGSHKPNAAVPMAYYNREAGTKRMTTETGAGQWGSALAMACQMFDLACRVYMV
                     RVSYEQKPYRRLMMNTWGAECIASPSNLTQVGRNVLAEYPDTPGSLGIAISEAIEDAV
                     NSPNTHYGLGSVLNHVILYQSIIGLEAQKQMKKLGLYPDVVMGCCGGGSNFAGIATPF
                     ACDKIHGKEVKIVGAEPTSCPSMTKGPFAYDFGDLAKSTPLLPMYTLGHDYVPAPIHA
                     GGLRYHGMAPIVSHLVREKLVEPRAYDQLKTFDAGVKWARSEGFIPAPETNHVLAAVV
                     DEAMRAREEGKEKIILFNWSGHGLVDLASYDAYLSGKLEAYELPDHEIERALNAIKDF
                     PKP"
     gene            19338..21605
                     /locus_tag="PLA58_01150"
     CDS             19338..21605
                     /locus_tag="PLA58_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOR61430.1"
                     /translation="MTKNTDRMSGRLTVALLFGVLLAGMTAPPSAAADPHFVIAASAQ
                     SPPYSYLDNKNNPQGILIDFWNLWARKTGAEASFALTSFDKAIELVRDGKADFHAGIF
                     YSEPRARHLDFSTGFLDDTLSLFVLDKLDVYSMSDLKNVPVVVGISKEYYAVEYMKEH
                     YPFIGIRLYNNNEELLHAALRREIVAFVVDYPVAFHYLSKNDSLGRYRVIQDISSQKL
                     RAAVKKSNRATLDFINNGLKKISDRELGDLADKWGLRQNSVIPPWFKKVLLGGVVVVI
                     LLAFFIHWALLRKEIRKQTSDLAEKNKILTLMQRDMMEVNRTTQNLMEDLEKISRDNE
                     QMLDMIVREMKETLERIQRATEDGDCAGPALMEIHDQSKNVLSLIEKFRQVSPVDAEN
                     YSQEMRKINLTEFFSKKKKDFKNLSLLKGIDLHMDMPAKTVTAMIHPGHFDELCNNIF
                     LSTVKYSRAGGQIRISLALAGTTDEKKIVIQFGYHSLAGGETREDGSSGASDRELPPV
                     ESASGIRLALIKKLILLQGGKISAFERERGERTIRLELPAVEEIVKSYEDIEALAHTG
                     DIILFKGLYYNRHGEKSVATDWTHVGLIVRLPGNPLPLIWESTPLENVPDQELGKGKS
                     GAQLVSLKERLENYETNVYAIRFLKVVRDPSMLNHLFRFISYAHTLPFPGELRLVGKV
                     IHAKIFSRLYRMKRRYKNIFCSELVAESYMVMGLLPEVPPPSAYMPVDFSTQKKLPLL
                     KGAHLSGEFMISFEK"
     gene            21801..22817
                     /locus_tag="PLA58_01155"
     CDS             21801..22817
                     /locus_tag="PLA58_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOR61431.1"
                     /translation="MMNMEIAVIVIFAFLTIAILMARPLIKIRRMSSVTNRAPEPHKP
                     QLTHNLYRHVRALAHQIGSRSLSEYDKLKEAQTYIETVLRESGIPYSIQDCPAHEKTC
                     ANIVVTFPGSRPLPQKILIGAHYDTIPGTPGADDNASSVSVLLEMCRALKNSTPDKTL
                     QLVFFTLEEPPSFDTERMGSRVCAGEARKNNEDILLMICLEMIGYYSPRKNGQEFPLP
                     LMNLFYSAVPDFVLVAGDLASRKMVAFAGDCIRRTSGLRVETLTVPQCFPGIKLSDHS
                     SFWRRGYRAIMITDTAFYRNPNYHTPMDTIDTLDFKAMTALCETLIKMVHELDGRPDD
                     GNCR"
     gene            23058..24467
                     /locus_tag="PLA58_01160"
     CDS             23058..24467
                     /locus_tag="PLA58_01160"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOR61432.1"
                     /translation="MKDTVEKRLEKKVQFYETVLNNMLNGVIITDLEGKVIFFSESYG
                     RFLGKDPKAQIGLHTTEVVENSRMHIVAKTGIPEINHAQRIMDHDMVVQRIPIKDHNN
                     KLVAVFGQVMFEDVRDVQVLANKLSILESKVKLYEKELVNLRASKYSCRNIIGKSSIM
                     TELRKLAVKAAKTNAPILLMGESGTGKELFAHAIHHASDRQAAPFVRINCAAIPKDLM
                     ESELFGYEPGAYTGAGNKSKPGKFELAHQGTIFLDEITELPLEMQPKLLRVLEEKELE
                     RLGGTRVTKCDFRLIAATHENLEKRVREGLFRQDLYYRINVIPINIPPLKERKEDIAM
                     IAQHLVLTMGKECGTNISGISPEVIRIFEKYDWPGNIRELSNVLERIFCSIEGVEDKI
                     CVEHLPVFFRGLAKKPSPSTSIILKNIREDSEKEALLQAIQISNNNKNQAARLLGIHR
                     TALYKKMKKFDIPLIHSKS"
     gene            24681..25208
                     /locus_tag="PLA58_01165"
     CDS             24681..25208
                     /locus_tag="PLA58_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61433.1"
                     /translation="MSSKAAGVAPRRSLTFREGGKHQMITIGDQKRDVIVVIMALLWA
                     FVLYAAWSGAVNKNSGLILCGFFASALIVGAFYLLGAVTNEKMPVGVLVYPVIFNVVC
                     WVIAFVMAYQTRGQKLDFILGMHPGMFGAIVFFWIGSMVAAAVGLLVWFDKHILPPDK
                     WAEFENEVTQQEKLH"
     gene            25224..26708
                     /locus_tag="PLA58_01170"
     CDS             25224..26708
                     /locus_tag="PLA58_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61434.1"
                     /translation="MNEQFIQPSPLIIGYTIVFILISMGIGFYAGKYAKTAEQFFGGT
                     KMFGGVTIGLASMAAVMSAFGFIGGPGLVYKFGMSSIWMTIACGPGMAYGYWILAKRM
                     RGMADVTDVATLPDIARVRFNSQAVRAVLAIGLFLAAISYLSSQVIGGGILMNQMLGV
                     SNEWGIVIMFGTTVAYMIFSGMSGSILTDAFQGFVMLIAVIAVLVGFFMMTGGDLTPI
                     MNSPQFGPSFVDGFGSMPLQAALVFGLIFFVGAMGQPTMLAKMYSLKTPQDIKKAGVI
                     GGLSYGVTSIVWILCGYGALYIVATGASPALAKADNAAFLFVSKLGGVTQALVMAGLL
                     AAIVSTSSFFISLSSSAITRDLIGAFGHEIPHDKQIRIGQILQVIVPLIAIGMGLWGG
                     RMVAILGALGWGFFASVTIPTFAIGMLWKRCSTEGILTGLVFAVISNPAYLILENLKI
                     FKFPFAGYLFCIAGAVVLTVVVSLFTKTSAGDNMPKALKPIFKL"
     gene            26773..26997
                     /locus_tag="PLA58_01175"
     CDS             26773..26997
                     /locus_tag="PLA58_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61435.1"
                     /translation="MKKFLSIRLTVALLACAVFFVACNGNGSKKKVASEDQDFSSSGM
                     IKDMEEMRKAAEKGKPTAPPAGVAPQESRK"
     gene            27053..29044
                     /locus_tag="PLA58_01180"
     CDS             27053..29044
                     /locus_tag="PLA58_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HOR61436.1"
                     /translation="MADSPTHHSKDSSQKKEKVVSAADAVKTIRDGDVIATEGFVGAG
                     FAEELAIALADQFLNTGTPRNLSIIYAAGQGDGATAKKGLNHLALEGLVGRVVGGHIG
                     LAPKLQTLIKENKILAYNWPQGVVSHFFREVAAKRPGVISRVGLGTYIDPRYDGGKLN
                     EKTFREGADLVKLIEIEGQEYLFYKTFPIHVAFIRGTTADTFGNMSMEKEALTLEALA
                     IAMAVKNSGGIVIAQVERVAEQGTLNCRDVKVPGIMVDYVVVSRPENHWQTFAVQYDP
                     SLSGEVRKPMKYIPPMAMGPRKIIARRAAFELRPKSVVNLGIGMPEGVANVANEEGIL
                     TSLVMTAEPGVIGGMPNGGLNFGTGTNTDCLIDQPYQFDFYDGGGLDVAFLGSAEIDK
                     HGNINVSKFGPRFVGPGGFINISQSTKEIVFVGTFTAGGLKVSVDEGKLTILEEGRER
                     KFLNHVEQVTFSGKYAAAAKQPVLYVTERCVFSLVEDGIALIEIAPGIDLEKDILANM
                     EFKPVMKTPPKLMDERIFQHGLMGLRKELVVIPLEERLIYNPKRNSFYVNFSNYNIRT
                     SEEIRRIKNGIEAILAPLGKKVPTIVNYDNFNIAPELTHEYADMLKHVMQYYSNVTRY
                     TTSAFLLMKLGDELLKRDVDLQMFGSIEDAKEALNTLEK"
     gene            29902..30357
                     /locus_tag="PLA58_01185"
     CDS             29902..30357
                     /locus_tag="PLA58_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOR61437.1"
                     /translation="MTRSEFKKIIANAIKAEVAANKFYSGVAKKAKDANLRKLFQELA
                     GEEAQHKLTLKGYMTKGAAELQFSESADYRVADELPTPPLTPDLKPVDGLVIAIKNEL
                     AAMQMYTQLAKASADKAQKKVFLELASMERGHKKRLEDIYTNMAFPEAW"
     gene            complement(30464..30967)
                     /locus_tag="PLA58_01190"
     CDS             complement(30464..30967)
                     /locus_tag="PLA58_01190"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOR61438.1"
                     /translation="MRKVWLTVLGGLLSVVFCLSVAAQTPVLSDGAGDALIKSQVETA
                     VSLLAAIHARQQKGEMSAEKAKELAAGLLRELRYGKEGYFWADTTEGVNVVLYGRKDT
                     EGRNRLADKDAKGVFYVKEFLARGKTGGYVEYLFTKKGQATPLPKRSYVLLFKPFGWV
                     VGTGYYR"
     gene            complement(31017..32123)
                     /locus_tag="PLA58_01195"
     CDS             complement(31017..32123)
                     /locus_tag="PLA58_01195"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HOR61439.1"
                     /translation="MFLKAVFPGKYIQGEGVLAQLPDAIGPFGKKGLILASPSAQNKI
                     LVPYRENLKTRGILIEPFSGECCEKELSRLADVLRRERVDVLVGMGGGKAIDTAKIAA
                     DRAGLPVMIVPTIASTDAPCSGCAVLYSEQGTFESVFYQKMNPQIVLVDTRVIAEAPA
                     RFLISGMGDALATWFEARSCRRTQSPNECGGLSTLAGLHLAKLCYDTLLSYGRAAKMA
                     AAQHIVTPALNHIIEANILLSGLGFESSGLAAAHSIHNGLTALPQTHAFYHGEKVAFG
                     VLAGLHLTDAAPDEIQTVYDFCEAIGLPTTFSDIGLADANRGMIDKAACKACASGESI
                     HHEAGDITPEKVLQAMLAANAMGEARRRSGQNQA"
     gene            32260..33855
                     /locus_tag="PLA58_01200"
     CDS             32260..33855
                     /locus_tag="PLA58_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR61440.1"
                     /translation="MNQPSRPVKIDFNSEFQRAFSLMEDTQKNILITGRAGTGKSTLL
                     NYFRDATQKKVVILAPTGVAAVNVEGQTIHSFFHFKPNVTPASIKRKKKGEKDRPTLY
                     KKLTTIVIDEISMVRADLLDCVDKFLRLNGPHEKRPFGGVQMIFIGDLYQLPPVVSSA
                     EREIFKSHYPTPYFFSAKVFDQLDIEYIELEKVYRQKDDEFVRLLNTIRNRSVTDDDL
                     ARFNARCDPLFEAPPGSFYLSLTSTNDLADAINEKRLAELPGKIWRAAGRIEGDFGKE
                     YLPTAVDLKLKKGAQIMLLNNDSLGQWMNGTIGKIRRFEQEDDGEEVIVAELDNGDTA
                     RISPYTWKIYRFFLKNEELRSEEVGSFRQYPVRLAFAVTIHKSQGKTFENVVIDVGRG
                     TFAHGQMYVALSRCTTLDGIVLKRPLQKNHILMDWRVVKFLTNLQVAQAAKTFSREEK
                     IKWIEEAIAEKKNIEILYLKGQDEKSRRTVRPLFMGNMEYKGYPYLGLEAFCLERREK
                     RIFNVDKILEIGEGKSSENKTGF"
     gene            complement(33893..34120)
                     /locus_tag="PLA58_01205"
     CDS             complement(33893..34120)
                     /locus_tag="PLA58_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61441.1"
                     /translation="MRAITGVSALSGVMNGHRSVTLRRESFAAATRKGGKEGHDPSVA
                     RIDRLISAAGAVALALSAFYFAPIVGSIFLK"
     gene            complement(34149..34736)
                     /gene="lexA"
                     /locus_tag="PLA58_01210"
     CDS             complement(34149..34736)
                     /gene="lexA"
                     /locus_tag="PLA58_01210"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOR61442.1"
                     /translation="MKKIRDIKSVEQSLVSFYRHQKRMPTYAEMMDLFEVRSKSVVFY
                     WIEKLIEKGILEKDAQGFLKLSGVSFGIPLVGNVAAGIPSSAEESTRDILSMDEYLVT
                     RPEASFLLRVTGDSMIGAGIMEGDLVIVERERTPRSGDIVLAEVDGRWTMKYFRRQGG
                     KVVLEAANPAYPAICPREELKIGGVITASVRKYLM"
     gene            complement(34839..36452)
                     /locus_tag="PLA58_01215"
     CDS             complement(34839..36452)
                     /locus_tag="PLA58_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HOR61443.1"
                     /translation="MMMLLAVTMIAACERSPQAGNAGSKGDKPPAYGDILVRGDIGDA
                     SNLIPLLASDSASHAVGGMVFNGLVKYDKDMNIVGDLAESWEVSPDGLSITFHLRKGV
                     RWHDGRPFTAADVLFTYQVTVDPKTPTAYAGDFQKVKKARILDDYTFRVTYDKPFAPA
                     LISWAAAMMPAHLLAGKDITQSPLSRHPIGTGPYRFKEWVPGQKIVLVSNEDYFEGRP
                     YLDGRITRIIPDTATMFLELRAQNLGMMGLTPLQYTRQTENNLFRENFNKYRYLAFAY
                     TYLGYNLKNPLFADRRVRQAISHAINKDEIISGVLLGLGKPATGPYKPGTWAYNDKVK
                     IYSYDPAKARELLKQAGWADANGDGLLDKDGRSFAFEILTNQGNETRQKCAEIIQRQL
                     AEVGIAAKIRVLEWSAFVTHFINKRRFDAVILGWTIPPDPDAYDVWHSSKTAPEELNF
                     VSYSNPEADEMLEKGRSTFHQDERKKYYDRFQEILAEDQPYTFLYVPDDLIIISNRVR
                     GIEPAPIGIGHNFIRWYVPKDEQKYTMTR"
     gene            36645..37295
                     /locus_tag="PLA58_01220"
     CDS             36645..37295
                     /locus_tag="PLA58_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOR61444.1"
                     /translation="MKVKTQELFNFYSHFAGAVAAIIGTVYLAIAASDSASGLVTALI
                     YGISVVFLFSASTLYHAFKKEENELSFWRKMDRLAIFFMIAGTFTPICYFCLDGAYKW
                     MMIAFQWSLVGVGLISQIFFPGAPRKLYAVIYLFMGWSALFTLKQMLANMSSSQAILL
                     VSGGVSFTIGAIIYAVKKPRMIPGIFSFHELFHIMVLIGGILHYAMIYGVYSHPGV"
     gene            37298..38230
                     /locus_tag="PLA58_01225"
     CDS             37298..38230
                     /locus_tag="PLA58_01225"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOR61445.1"
                     /translation="MSASSPPAPPGPATNLLLAEELKLFLQDYEKNYKAGESKPPEVG
                     MPFLLIAPESDTGVLLIHGLMAAPAEVREWAEFLHARGLTVYAPRLSGHGTSSGDLST
                     RNANDWLSSVDRGHALLKTCCTKILVAGFSTGAGLALQSAILKPRDFEAVIAVSAPLR
                     FKSFSSRFAELLHGFNLLCANLGMDRLAVKFMKNDADNPHINYLLCPVSSFVQVKKLM
                     RSVGRRLADIQIPALILQGRQDPKVAPKSGPAIYERLGAHQKRFAWIEFNRHGIVLGP
                     IAREVFKEVESFLGACRLIDSPKQRNARIGQAQK"
     gene            38494..40023
                     /locus_tag="PLA58_01230"
     CDS             38494..40023
                     /locus_tag="PLA58_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOR61446.1"
                     /translation="MAEGEKDRAALRKTRKESVQMRAAGAWDIVYDAVNNFSLNGDAN
                     QAAAVALYAILSAIPLFILTLIAAGSFFSSNTTLQTDIVGAVQGFHPYFSETLLQQVG
                     QIEGKSKVLGWIGVLGLVWLSAAIFNAVESALNIIFRSRHKRNYFVSKLMAIAMIPAA
                     WIVGGLSILITYMATLLAKEPTASADGLLLSLTMPAQIGLRYIVPYLMVVILVAFVYR
                     IIPTVKIRPSVALAGSAIFAFFVEIAKQLFTWYVANYTRYNVIFGSLETVVILVIWVF
                     YVALIFLFCAEIMSSFERRDMLLLERALLKPHHSLLKVDARLFQKFGRIYAPGEVVFN
                     ENDAGSEMFYVISGRVRLERVDGRVKKTLAEMGPGQYFGEMAALIDVRRTATARALEN
                     SHLAVIDDRTFQNLIRESREVALAMLREFASRLKNSNAALEEFTHRRTQMLILLQILD
                     QPKATVDEHIEQISRLTRKEPSQIRNIFQELSGQGIVRLRDNRPDIDRDKMWSMFDSG
                     I"
     gene            40057..41823
                     /locus_tag="PLA58_01235"
     CDS             40057..41823
                     /locus_tag="PLA58_01235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOR61447.1"
                     /translation="MKMILHPRTLHRIGPFILCLVLLWAITAQAGEAPPIRVGGAVPA
                     VEIASQSEYLLDYSGRLQYQDILSSGQPFQRHTKYSFQFSFKRATLWFKCRIAPADSG
                     DETALASRRSFLVFDNAALGAVTLYVPVILDGKPDLIEFHGGWRQGEKSREFPFLYPT
                     FVLPDNLDASRPVVVRVATPYALQFRATLYTIDAFRKMSFVLFLIVGFFAGILIAMIL
                     YNLALYLFIRDRQYLFYITYIGFLLLWQCVLVGLFQYIWPPLGAFLMSGIPVFAALMM
                     IFAVSFGIVFLNTPKTAPRHDKLLKGLAVFIGTIIALVLLRQLWLGNLLSYLSGQVVS
                     VVLFTAALSSLRSGFRPALFYLIAFGVFLLCAFVFFFKFYGLIPNNTFTMHIVIFGSA
                     AEAVLLSFALGYRIRIMQEEEEQLRERERDLEAISVTDELTGLFNRRFLNPALVKKTA
                     AARRSGTRLSLLMLDVDHFKDFNDTYGHPEGDKVLAALGGLLIQTLREEDIACRYGGE
                     EFVVILHNADLNAAMEAAERIRAGFEKISFRPDPKKEIFATVSIGAAELLPQETPEQL
                     LFRADQAMYQAKQTGRNRICSA"
     gene            42103..43113
                     /locus_tag="PLA58_01240"
     CDS             42103..43113
                     /locus_tag="PLA58_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOR61448.1"
                     /translation="MKAAVLRGAKDIRTETVPDPVCAPHGVIVKVNSCGVCGSDLHWY
                     KEDGHEGTIFGHEFSGDIVEVGKNVQGIAVGTRCTAVGFSPCGQCFWCKQGKAHRCSA
                     MALLGYQFPGAMAEYVHVPFAALGRSVFPLPEELTYEDAASVEPLSISYFSVNRGQPK
                     TSDIVAVIGLGVIGLYAVQILKALGVEKVLAAGRRPSRLAAAKRCGADPVIDAAAEDT
                     LQAVMQATDQLGVNTVVECAGNQATFDQAVAMARGGGKILLVGIYEEALHWQPLSVIS
                     KNLTLVGCLGGNFPAVIELLKSGKASSRGIISHRFPLSQAAEAFRTQLQDPTAVKVMV
                     QP"
     gene            43126..43821
                     /locus_tag="PLA58_01245"
     CDS             43126..43821
                     /locus_tag="PLA58_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOR61449.1"
                     /translation="MNELYLAHTEFIERDAPEIRAFAQNAVGDAATPREKAVKLFFAV
                     RDGIYYDPYRIDPRREAFRATTVLRQGYGYCVTKAVLLAALLRSQGIACRIHLADVRN
                     HLTTKRLKEVMKTDIFYNHGYNDVLLDGRWLKVTPTFNLSLCEKFKVKPLDWDGQSDA
                     VLHPFDMEGRRHMEYINDRGSFDDLPFDFLIETFITRYSGSIESFANQAAKTGQFEAE
                     AEDEARKLSANQT"
     gene            43818..45185
                     /locus_tag="PLA58_01250"
     CDS             43818..45185
                     /locus_tag="PLA58_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOR61450.1"
                     /translation="MNEMTDFSELKAILDERRVSSGESVRHLHSHDESFHAPSLPDVV
                     VWPHTTEEASRLVRWAKGKRVPVTAWGAGTSLEGNPIPVQGGMVMDFNEMNRIIAVRP
                     EDFQADVQAGVIYKELNKTLARDGLFFPPDPGAAATIGGMIGNNASGIRSVRYGATKD
                     YVMRLTVVLPDGEVIHAGNRARKSSSGYNLPALFTGSEGTLGIITEATLKLAGLPVNF
                     MAVRATFPTVLNATTTVYQIMRAGLAPAAMEFLDANVVRVLNADRDLTLEENPTLLME
                     FSGYSRQGLAEEMAFVEEICQQNGALSIDRGIGAEERARLWEIRHQTYESIKRYHTGL
                     SPLIMDVAVPLSRYSEMVDFSKNEVRDLTAYLFGHAGDGNIHVHVMDDPQDAKRWARV
                     EEATRSIVMKALEFEGTCTGEHGIGIGKSRFMETEHGASLMLMKRLKALLDPENLFNP
                     GKFFL"
     gene            45226..46101
                     /locus_tag="PLA58_01255"
     CDS             45226..46101
                     /locus_tag="PLA58_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61451.1"
                     /translation="MEDIIYRGVLTNIGELSFLAGENGSRHLEGEKIWSGYWSHWSGK
                     SVCARRLPQIDYETGKPVILLWPDAPVADASLVELYFNERLVKYPLSFLGHLAVLVNG
                     KVFNFSHWMNENEAMSPEEYFFRPALGEFAPDPASGRDNTEDSQRPYYDKFGRLFMRT
                     IHVLRISGLDTRRLSGFFFTELEKIRSTPPDPKEPGKYRDFHILTKSCATIIRDGFQS
                     LGFEKISGIFPRDLFVNMAYFFLKPLRLPNVQASLHTLRQLQVPEAAPSAMPPLLNPQ
                     NRLRYRTLRKEYDVG"
     gene            complement(46287..48761)
                     /gene="lon"
                     /locus_tag="PLA58_01260"
     CDS             complement(46287..48761)
                     /gene="lon"
                     /locus_tag="PLA58_01260"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOR61452.1"
                     /translation="MTEQNVPENKNTFNIPEVIPILPLHNVMVFPKTMIPLEVTGSAS
                     VLVDEAMTKDRLVGLIMAKKEQENIQPYAREDLHAMGTCAVILKMAKTAENRTQLLLQ
                     GLSRFSVEEIIEGKPYQQARIRLVDEKEVKDLETEALMANLLTLFDRILKLSPFLPPE
                     FGPMAKSISEAGTLADLIASVINAPVEEKQKVLDTADVKLRLKELTKMVNHQMEVLEL
                     GNKIQTKVKDDIDKNQREYYLRQQLKAIRQELGESDENTVETDEYRKKIEEKNLPEEA
                     KKEALRELERLSRMHPSSAEYTVSSTYLDWITTLPWHEATADNLDIVAARKILDEDHY
                     GLSKPKKRIIEYLAVRKLKPDSKGPILCFVGPPGTGKTSLGHSIARALGRKFWRIALG
                     GVRDEAEIRGHRRTYIGALPGRIIQGLRRAESNNPVFMLDEIDKLGSDFRGDPSSALL
                     EVLDPQQNNTFTDHYLDVPFDLSKVLFIATANMLDTIPPALLDRLEVIELTGYTQEEK
                     VKIAEKYLIPRQLAENGLTASQFKLTGKALSTIITGYTREAGVRNLEREIANACRGVA
                     AQIAEGSLKSKTVTEKDVHRYLGAVRVPTDIDARITRPGIAIGLAWTPVGGDMLFIEA
                     TAMKGKKGLTLTGQLGDVMKESVAAALSFLRTNAEALGVNPDFFEDRDIHIHVPAGAT
                     PKDGPSAGVTMLTALTSLLTNRKVRKQLAMTGEITLRGAVLPVGGIKEKVLAAYRAGI
                     KTLILPAWNRKDLDDIPANVRKKVKFHFVSDMMEVLRLALEPAREKTMKQKAEKKAVK
                     PGSAKPAAKGDKSRTKIARKPVAKKK"
     gene            complement(48795..49235)
                     /locus_tag="PLA58_01265"
     CDS             complement(48795..49235)
                     /locus_tag="PLA58_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOR61453.1"
                     /translation="MTYIKINFGGGFEDEFQKAVDEVFHLVRPAFKNYECIWRPGVDV
                     CESADEVIVLADMAGLNKEELHIEVNRLTMKISGIRKAIQLLQNARYRQAEIPHGYFE
                     RSVALPAPVDAQSSVASYADGILMVRMKKLPVNKTHRISVISET"
BASE COUNT        11044 a        13801 c        13285 g        11234 t
ORIGIN      
        1 ctgataaaaa tagccctttg aaggcggttt gggatgagag ctttgaataa cggccatttt
       61 tgtcatttcg accgaaggga gaaatcttga acaaacggcc atccttaaga tttctcgtcg
      121 ctcacgctcc tcgaaatgac atttttcaaa gctctcggga tactaacatc ggcaatctta
      181 tttgacaagc atttcctgac accggtttcc cgcccgtatt gacagaaaca cggagaatga
      241 atatactacg gccaaatcgg gcagcggcat ttaaggtgtt tttatgattc gctattttaa
      301 cgcgacgcat ataccggtgg cggccagggc ctttagcgat gctgaaaagc tggtctcccg
      361 acattttcgc ctgagcgaga acgatttgcg caagaacaag tatgacgtaa aaacacttgc
      421 atttttagag gagcatgaag taaaagacgg cgcgtttgcg cacctttgta aatattctta
      481 cgaaaagcct tcggatggaa gtcctgaagg aaaagaaggg tttgactttt accgggtgtg
      541 tttgcaggac aatatcattc tggatgcggt ggaaagggca aatacattta taaaattcag
      601 ccctttgatg ttgtacattg ccgctcatga gctgattcac gtgctccggt ttggaagtgg
      661 cgccattgat ttcaatgcgt cactccagga aaaagaacag gaagaaaaga tcgttcacaa
      721 cctgacgagg agcgctttgc aaccggtaaa acaaaacgac ctggagattg ttttggactg
      781 cttctcgagt gagttttcga tcagtgattt atataattaa tgtgaggagg ttgagactat
      841 gcctatttat gaatatcagt gcaggaaatg cggcaaacaa tttgaggcgt ttcagggcat
      901 taccgagccg gatttgaaaa catgcaaatt ctgcaagggc aaggttcaca agatgatgtc
      961 gctgtcgtcc ttcagcttga agggcagcgg atggtatgca acggattatg cggggaaaaa
     1021 gccgcagacg gtcaatccga aaagcacgga aacggcaacg gcagaaagtt cttcggcttc
     1081 ttccgatgct tcagcaaccg tcaaaggcgt cagcgaagat taaatgtatc ttctcaaaaa
     1141 gaccaaataa aaaagcggca tcggaatgcc gcttttttat taaccacact ggagcaataa
     1201 aacgcagatc gctgttgccc caacatccct ttgagcggcc attggaaggg ttttgcataa
     1261 taaatcaagg tgatgcgaaa gatcagcgat gacaactgtt tgagccgaag gcgagttttg
     1321 tcatcgcctt tcgcgagcct tagacttatt caaaacactt ccaggcgagc gaaaagggga
     1381 tttggggcaa cagcgcgctt tctaatccca ccgtcaaatt tattcctgaa aaaacgcctg
     1441 taattccttg cacaactgct ccggcacaat cggacagatg atttcatcgt gcagtttttc
     1501 aaaataattc aaatcgctgg tgcccagggg attgatttca aaacgcggaa cgattttccc
     1561 ctgcacccag aagttcttct ccggcagcat cggcgcgtac agggaaagat tgaagctcat
     1621 gaaattattg acatacatgt gaagaaagac ccgggaaagc cccttgagca gttcctcaaa
     1681 gttggcctgg gtcagatcaa aaaacgaggt tttctccctg aaataaaaat ccacttcccc
     1741 ggccatgccc ttgggcgaga aagcggcaag ccatgacacc acaccggtgg acgcgatgaa
     1801 gcgctcgcct gcgtcctgct cacgatccag caggtctttc caaagattgc cgccggaggc
     1861 agcttcgtaa ttctgcgcgc tggccagcag tcgccgcatg aaactggtcg gctttttgcc
     1921 cgcgatcgtc tgcaggtggg gatggatcag tccgcctccc gcgggcggca tgtagttcca
     1981 gttgatcgaa ccataccgga accctttctg cagttcggcc atccggtaaa aataatcccg
     2041 gcagacggca aagccgtcgc gcatggcgtc gggcgtcaat gagtccagcc ccacaaagtg
     2101 gcggtgcgaa aaaatcgcca ccgtgttggt cgcgtcgtag ggaaatgcgt tgggaaacag
     2161 aaaagcgctt cctcgggtga attttccgcc ttcgatgatg tccggagtga accggggcgt
     2221 gattttgtca aagagcgccg ggcaaaacgg acagattgat tccggcgatt tttccagata
     2281 gaccgcggtg tccggcttct gggccggcct tacccggtaa ggaagaatgc ggctcgccac
     2341 gccggtggtt tcgtcgtacc gctcttcaat ggtcatgtct ttgggttcaa atccgtcaaa
     2401 ggggctgagg tgccgtccgg tgaccgtttc cttgtgaaat ccgatatctc tcatgttccc
     2461 tccttcccgt tctcagtcgg cggcggctcc tctttgcgcg aagcgctttt tctcaggcac
     2521 tggtgaagaa agcgctccac gctctcatgg gataagtccc cttgcagctc cgccttgaga
     2581 acgtccagtg gaatttccag ccgggcggcg ctgcggtgtc cgcccccctg gccgatctga
     2641 ccgcaggctt tctgcgccat cgcgccgcag tcctggcggt agccgtcgcc ccggaaaata
     2701 atgaccagcc ggtcgttcac gatgcccgcc accaccacat acgacgtgcc gatcacgtgc
     2761 agataaaaat ccgccacctg cacgcagaca tccggacttt ccacattccc cagaaacccg
     2821 acgcggcgtc cgcggaaatg cgtcatggaa tgaaacgcgt gatcgaaata tttcagatag
     2881 ctgcgcgggg tctgattgag ctcgatccgg cggatcagcc gcaggttggc gcgccgggaa
     2941 tgataggtgt aggcgcggat gtcttcggcc agcgtttcgc ggtcgaaatt atccgtgtcc
     3001 gtcttgatgc cgtacagcag cgccgtgtgc agattgcggg gaatgcgcac gcgcgcggcc
     3061 agcaggtatt ccgtgagaat cgtcgagagc gcgccgtacg aaggccggat gtcggcaagc
     3121 tcggcctgcc agccgccttc gcggggatga tgatccataa cgatgtccgg ctcgcgcgcc
     3181 gggagggaat ccccaaaaaa agaaggctgc gcgtccacca gcgcaacaag ccggcaggcg
     3241 ccggcgtcga tctgattcag aagccggatg ttgaggcgca tcgcctgcgc cagttcccgg
     3301 ttctgctggc gccggatcgg ctccgtcgcc gcaaaaaccc atttggacag gcctttggtg
     3361 cgcaggagga tttcgcgaag cgccatggcg gaagcgatcg cgtccgggtc ggggctgccg
     3421 tgcatcaaca ccagcagcga atcgtccggc tgcgcgagct cgaggagctt tttgacattc
     3481 tgctgcgtct cacttttgaa gggcaaacga tgcaatcgac gttactccaa gagaattttc
     3541 cccgaatgat cggccacgac gtcgcccacg acggccgcat ccgtgatgcc cgcccggcgc
     3601 atggcttccg cgagcggctc ggccttcccg gaaggcaggc tgaaaaacag accgcccgcc
     3661 gtctgcggat caaagcagac gtcaaaaatc cagtccggac aggacggggc tttttcgacc
     3721 tgatgaagac ggtagttccg gttgcggtaa agaccgccgg gaagatagcc ggattccacc
     3781 agatcccgca cgccctccag aatcgggatc cgggctgcct cgatccgcag accgacatcg
     3841 ctgccttcaa tggtttcaca ggcgtgtccg agaaaaccaa agcccgtgat atccgtgcag
     3901 gaatggatgt tccccgcggc gatcatcagc tcggccgctt ttttattgag cgtcgtcatg
     3961 cagcgtgtcg cctgttcgat gagcgcctgg cctaccgccc ccgccttgat ggccgttgcg
     4021 acaatgcccg tgcccaacgg cttggtcagg atgagccggt cgcccgcgcg ggcgccgcgg
     4081 ttgaaaagca cgcggtccgg gtgaatcgtg ccggtcaccg acaggccgta tttgatttcg
     4141 ttgtcttcca cactgtggcc gcccaccaga agcgcgccgg cttcgcgcat cttgtccagc
     4201 ccaccgcgca aaacgtcccg cagcaccgat ttgtccatcg ttttcacggg aaaacagacg
     4261 atattcatgg cggtgagcgg agttcccccc attgcgtaaa tatcgttcat ggcgttggcc
     4321 gcggcgattt ggccgaaggt gaaggggtca tccacggtcg gcgtgaagaa atccacggtc
     4381 tgcaccagcg ccaggtcgtc ggaaatttta tacacgcccg cgtcttccgc atgttcatac
     4441 ccggtcacca gcttcggatc ggaaacaagc ggcaaatcgc agagaatgtc tgaaaggtcc
     4501 cccggaccga tcttacaggc tcaacccgcg ctcgatacgg tttggctcaa acgaacggac
     4561 tttttttctt ccatggttcc tcaactcgaa aaatgatttg cgcgcccctg ctgaaggcgt
     4621 ttgatttttt agcatagaag ccgttatttt ccaatggatt tcagaaaaga caaaactctt
     4681 tacgttccct cgaaaaagac aacggtcctg attccgaacg gccggtttgt ccgatggcct
     4741 gccggagggg ccgcagaacc tgcggataag acaaaacact ttctccgacc gcgccaacag
     4801 aacacccgga cggcaacccg gcaggagggc attgtttttt gttgatttat ttgcgccatt
     4861 cagattatag gaaatcacct ttgaaaggga taccgggcat gtccattcgc tactacagca
     4921 ccaatcgcca tgtaaaaccg gctgcgggaa ttactccctt taccggaagc gtgtctttcc
     4981 gggaagccct gctggcgggg cagtcgccgg acgaaggcct gtttgttccc gacgccatcc
     5041 cgcaacttcc catgaaagac attgttgcgc ttcgcaacca accttactgg caggccgcac
     5101 tgctggtcgc ccgggcgttt ctgtcggaag aattttcttt agaagtcctc gagacgatcg
     5161 tgaaggacgc gtacaacttc cccgtcccgc tggaggcggt gtatccgcgc gccttcatcc
     5221 ttcggctgga ccagggcccc acggcgtcct tcaaggattt cgccgcccgg atgatggccc
     5281 ggctcatgag ccactgccgg cctgaagggg agaggctcaa tattctggtg gcgacgtccg
     5341 gcgatacggg aagcgccgtg ggagaagcct acaagggcgt tgacggcatt gatgtgacca
     5401 tcctgtatcc ggcggatgaa gtgagcctga tgcagaaaaa gcagctcgac accatcggag
     5461 gcaacgtacg ggccatatgc gttcgcggaa aattcgatga ctgtcagaat ctggtcaaag
     5521 aggcgtttgc cgacaaagat ctggcccgtt tcaatctgtc gtcggccaac tccatcaatt
     5581 tcgggcgcat tctgccgcag atcgtttact atatctatgc ctacgcccag ttggccggcg
     5641 ccggcgagga agcggtggtc tccgtcccct ccggcaattt cggaaacgcc ctgggctgcg
     5701 aatacgcccg ccggatggga ttgccggtgg caaaactgat catgcccacc aatgaaaacg
     5761 acgagtttcc cgcttttctg gaaaccggaa cctacgcgaa agtatcgccg tcgcgcgcct
     5821 gcatttccaa cgccatgaac gtggggcatc ccagcaatct ggcgcgattc tttgaattgt
     5881 acggcggcac cgtggaccgc acgggcatgg ttcaccgcta tcccgacctg gatgaaatgc
     5941 ggcgccgcat tttttccgtg agcgtcagcg acggggaaac ccggcaaacc atcaaaagcg
     6001 tctacgagcg ctatcgcgtg gttctggagc agcacggagc ggtcggctgg gcggggctta
     6061 cggcctacca gaaaaaaacc ggcgacacga ggctggccat ctgtctggag accgcccatc
     6121 cggcgaagtt tcccgatgag atcaaggacc tgctggggat cgaccccgaa ccgccggaaa
     6181 gcatgaaaga cctggcccga cgtgaaggaa gcgccatcaa cctgtccggg gatttcgcct
     6241 ccttcaaaac gtacctgcag gcgcatctga aaatcaaaaa ataaacccct ggacgtccgg
     6301 ccggcgaatt ccggcagacc gtgtggcgac ggatgaaaaa accggcaacc cctcccaaaa
     6361 accgacagac gacggggcaa gatttttctc ccggagacct gatcgttgaa accggccgct
     6421 ttttcctggg cgcgccttac cgggaaggca cgctggaagg accgggccgt gaaaagctga
     6481 tcgtcaatct tcatgcattc gactgtacaa cctttgtgga aaccgtgctg gcgctggtcc
     6541 gctctgcccg tttcggaaag ctccgcgcgc cggaattccg caggaacctc cgatacattc
     6601 gctaccgcgg gggaaaaatc gacggctatg cttcccgtct tcattacttt acggactgga
     6661 tcattgataa tcagaaaaaa aagacgctga aggacgttac gcggcatctt ggcggcgccc
     6721 gccggcaaaa agaaattcat ttcatgacaa cccatcgcga acgctacgcc gccctgaccc
     6781 atcaaaacca ggcgaaaaga atgcggcaga cggaacggag actgtcgcgc aggtcgtttt
     6841 acatgatcgg caaagaacgg gtatcccaaa ttcaggacgg cattcagccg ggagacattc
     6901 tcgcatttac gacaaatcag gacgggctgg atgtggcgca tgtcggattg gccgtccgcg
     6961 agcgcggccg tctgcgcctg ctgcatgcct cgcgccggga aggcgccgta gtggtttccg
     7021 ctgaaacgct tccggcgtac ctgcgccgcc gtggaaattt caccgggatt ctggtcgccc
     7081 gggcactcta gcgccgcccg gcgaaaaatc ctgcgccgcc gtttttttgc tcattttcac
     7141 ctttattttt gagcgcacat atattaagat aaagacaaat caccgaaggc aatacaggag
     7201 gcgactatga actatgtcag ggatattctc aagacgaaag acggcaggat tcagacgatt
     7261 tctcccaagg caaccgtata tgaagccctg gaaaaaatga gcgagaagga aatcggcgcc
     7321 ctggtggtca tggaaaacaa gaaggtggtg ggcatctttt cagaaagaga ttacgccaga
     7381 aaaattatcc ttcaggggaa aacctccaag aaaaccctgg tcaaggaagt catgtcttcc
     7441 catttgttct ccgtcacccc ggacaccacg gtggaagacg cgatggtcct gatgacggga
     7501 aagcatatcc ggcatctgcc ggtttttgaa aagtccaaat ttgtcggcat catttccatc
     7561 ggcgacgtcc tgaagctgat tatctccaat aaggatttcc tcatcaatca gctgagcgac
     7621 tacatcgccg gaaaatatta ttagaccgca aaccattctt ttccccgaag acggaatttc
     7681 caggcgtccc ttcggggaaa atttttttcg ctgtccgcgc cgcgcgtccg accatcctga
     7741 tcgggtcggc tttcccacaa acctgccctt cctgcaagtc cgcctcctct tttccaagag
     7801 gcgttttttc gccttgttcc ttgacatccg tcgtcaaata ccgttataca ttgtcgccca
     7861 tttctttcaa actggaagcg tgccgtcatg gatatggtca acgccgctgc atccgtcgtc
     7921 aaaaacatca gaattcagga catcctggat atggcgatca tcgccggaat gattttcgcc
     7981 ctcttgatgt ggttcaaaac cagggcgtcg agatttgttc tgatcggcat tttgcttctg
     8041 ggcggcgtgt atctggccgc ccggtttttc cagctctatc tcaccgtgat cgtactccag
     8101 gggttcttcg ccattttgct gttcgtgctg gtggtgatct ttcaggaaga tcttcgcggc
     8161 ttctttgagc gcatcgccat gctgggccag ctcggcaaac aggcgccgcc cctgtcgcgt
     8221 ctgcaaaaaa ccgcgaacat catctcggaa accgcgggca atcttgccaa gaagcgcatc
     8281 ggcgccttgc tggtcatcca gggaaatgat ccgctggacc gccatctgaa cggcggcgtc
     8341 cccctgaacg gcgccgcgag cgagccgctt ctggaaagcc ttttcgatcc tcactccccg
     8401 ggccacgacg gggcggcggt cctcaaagac gaccgcgtgg caatgttcgg ctgtcatttg
     8461 cccctgtccg tcaataccgc gcgacacgga aacatgggcc tgcgccacac ggccgcgctc
     8521 ggactgacgg agcgatcgga tgccctgtgc gttgtcgtgt ccgaagagcg cgggagcatc
     8581 tccgtctccg agggaaagac gctctccgtc gtccccggcg cctcggcgct ccacgaaatc
     8641 ctggaaaagc actacgcaaa ataccgaccg gcgccgaaaa aaagaccgct ccgggcctgg
     8701 ctcagtgaaa acacaaagga aaaagtaatc gccgtcggtc tggcctgttt gttgtgggcg
     8761 gccttcggtt atcagcggga taccgtgcgc cgtgattttt tcgttcccct ggcgtataaa
     8821 aatctgcctg ccggatggca ttttgacgcc cccccggtga aggaggccaa agtgacgctg
     8881 caggggcccg aacaggcctt ccggctgatg aatgaagatt cgctgaagct cgccctggat
     8941 gtatcggcgc tggcgcagaa aaatcaggaa ttttccctca accggaacat gctgaacacg
     9001 ccaaccggtt tgtcgattgc cgaaatcagc ccggcaaaaa tcaaggtttt cgcgtcgggg
     9061 caggttgtcg cgcatctgcc ggtccacgtc gcgaccgtca accggctgcc ggatcacctg
     9121 gtcctgcaga aaatatccgt gaagccggcc gcgctccggg tcctgctgga ttcgtggatc
     9181 aagcccgccg gccttcggct ggaaacggag cccctcgatc tgcaaagcgt cacttcctcc
     9241 atgacggtgg cgctcagggt caatgccgtc tccggcgcat ctttcccgga cggaaaatcc
     9301 gccagggttc aggttgccgt ccgcgtcaaa cccaaggagc caaccccatg accctcgccg
     9361 tgaaaaaaag atcgaaaaag gccggactga gcccggggac attgattcat atcggccacg
     9421 cctacgagga ttcatcccgc cttcggctga tgcgctacga cgaagccgcc ctggtggaaa
     9481 aggaaaactt ctcgccgggg gagctgatcg aggaaaagaa aaaaccgggc gtcctgtggg
     9541 tccacttgga cggtctgcgg gacatcgggc gtctggagga aatcggccgc atcttcggcc
     9601 ttcatcccct gattctggaa gacatcctca acaccgatca gcggccgaag gcggaggatt
     9661 tcacggatta tattttcatt gttttaaaga acatccatca tcccgccggt cagaacctgc
     9721 acgcggagca aatcagcatt cttctggggc cgaatttcgt tttgtccttt caggaaaaag
     9781 cgggaacgct gtttgacccc attcgtgaaa ggatccgcac cgacaaggga agaatccgca
     9841 aggcaggggc ggattatctg gcgcatcatc tgatggacgc aatcgttgat cagtattttg
     9901 ccgtgctgga agacatcgac gacaaaattg aactgctgga agaggacctg gtcaaaaaaa
     9961 ccactcccga caaactgcag gccatccacc tgctgaaaag agagctgatt ttgctgcgca
    10021 agtcgctctg gccgcttcgg gaggccatcg gtctgctgca gcgggctgat tccccgctca
    10081 tcgccgattc cgatctgatt tatttcaagg acatttccga tcacatcatc gccgtcattg
    10141 acacggtgga caccttccgg gacatgctgg ccggcatgct ggacatctac ctgtcctccg
    10201 ccagcatgcg gatgaaccag gtcatgaagg tgctcaccat tatcgcgacc attttcatgc
    10261 cgctgacgtt tctggcgggc atctacggaa tgaatttcgc ctacatgccg gagctgggct
    10321 ggcggtgggg ttattttgcc gtcctgggcc tcatgttgac gattgccctg ggaatgctta
    10381 tgttgttccg caggaaaaaa tggatttgaa acagccaaaa aggttgcaat agccgttaaa
    10441 atatgttcag ttttcagccg taataatatt ttttcaggag gaaaccctat gcaggtattc
    10501 gcagcaagtg acatcgtcga agtggcgatt cgaattgagg agaatggaat caatttttat
    10561 aaattcgccg aacagatcgc caggcaggaa gacgccaaaa agctcttttc ccagctcgcg
    10621 caggcggagg ccgcgcacaa acgcacgttt gaaaagtttt tcgccgacat ggaaaaatac
    10681 aatccgccgg agcagtatgc cggagagtac agcgcctatc tgcgcaacta cgtggacaac
    10741 aacctgatct ttaccaagga aatcatggac cggcagctcg caaaagtgac ggacacggtg
    10801 ggcgcctttg agtttgccat gcagcgcgaa ctggattcgg tcctgtacta ccatgaaatc
    10861 aaaaacatgg tccccgcggc acagcatgaa gccatcgaca aaatcattga agaggaaaga
    10921 cgacactaca cgatgctgtg cgacatgaaa aaacgctacg agaacaaata gcgcaacgac
    10981 gcccttgcag gcccggaccc aagccttatt cagccgttcg acaaaacgat gctccgaatg
    11041 ttgatttgaa aaagaaaacg gtgaataaaa tgctgcgaat tttgtatcaa ccctacaagt
    11101 ggttgatctt tgctccgtat ctggcggtct ccaccctctt tttcggctct ctgacgatgg
    11161 tgctggccgt tgtcacgaat ccccggatca ccagcttcat ctgcggaacg atctgggccc
    11221 gcctcaacgg ctatcttacg ccgatccggg ttacggtcac gggaagaaac catatcgtca
    11281 aaacgcagtc ctacgtcatc gtcgcaaacc atcaaagcca gtatgacatc ttcgtcctgt
    11341 acggctggct ggggatcgac ttcaagtggg tgatgaagca ggaattgaga aaagtgcccg
    11401 gcatcgggat cggctgcgaa aaagtcgggc atattttcat cgaccggtcc aaccatgaaa
    11461 aggccctcgc ctcgcttgcc gcggccaaag aaaagatcgt caacggcacg tcggtcatct
    11521 tcttcccgga aggcaccaga agcagggacg gccggctggg cgcctttaaa aaaggggcct
    11581 ttaaaatggc cgtggatctc cggctgccga tcctgcccat caccatcgtc ggcacgcgcg
    11641 atattttgcc gacggactcc gttgatctct ttcccgggcg ggcgcgcatg atcatccaca
    11701 agccgatcga taccgcgggc tatacggatg acaacctgga cgaactggtg gaccgggcgc
    11761 gcagcgtcat tgaatccggt cttcgggaaa ccgcagccgg gtgatgccgc cgaaaatcgc
    11821 ggcccccacg cttcctggcg tttcagcggg ccctctcagt ttttcaaatc gtatttcttc
    11881 agcttatacc gcagcattct ttccgacatg cccagaaatt ccgcggcttt ggtctggttg
    11941 tcggacgcct tcgccatggc ttcgccgatc aggcgcttct ccagctcttc gacggaatcc
    12001 cgcagcgtgc cggccgatga accgcccggc gccccggaaa aagaaccagg ccgaaagggc
    12061 aaatcactca cggtgatata atcgttgcgg gcgatgacga cggcccgctc cataatgttg
    12121 accagttccc ggatattgcc cggatagtcg tatttcaaaa gcagatccct ggcttccgcc
    12181 gtgatgcccc tgaggctctt tccgttttcc gcggcaaatt tttccagata gtgatccagg
    12241 agcaaaggca ggtcttcctt tctttccctc agcggcggaa gggacatggc caccaccttg
    12301 agccggtaaa aaagatcctc tcggaaggcc ccttcccgga cgcgcgcttc caggtcgcgg
    12361 ttggtggcgc tgatgatccg gacgtccacg gaaatgctgg cattgctgcc gagcctcgaa
    12421 atttccttct cctgcagaaa tcgcaggagt ttcacctgaa cggcggggct caattcgccg
    12481 atttcatcca ggaaaagcgt gccgccgtcc gcctcttcga agcggccgat gcgccgcgaa
    12541 ccggcgccgg tgtaagcgcc cttttcatgg ccgaacagtt cgctctccag aagattttcc
    12601 tgcagggcgc cgcagttcac cacgacaatg ggcctcgcgg cccgcgggct caactgatga
    12661 atcagcctcg ccaggagttc cttgcccgtg ccgctttccc ctgaaatcag cacggtggcc
    12721 cggctgggcg cgacgcggct ggccatgttc atcagctcca ccatgcgggc gcttttgtaa
    12781 atgattttct ccgcggtgac gccggtcttc cccagctcct gtttcagaag atcattttca
    12841 cggatcaggc cgcggcgctc cgccacgcgg tccacgagaa tcagcagttc gtcgagttcg
    12901 atcggcttcg tgatgtaatc caccgccccc gccttgatcg cctccacggc ggtttcgatg
    12961 gttccgtacg cggtaatgat cacgacatcg atctcgtgat tgatcctttt aacctccttg
    13021 agaacggcca gcccgtccat gccgggcatt ttgtaatcca gaagaatcag gtcaaaatga
    13081 tggttggcga ccgttgtaac cgccgcttcg ccgttctccg cttccatgac ccggtggcct
    13141 tcggaaatga gaaaatcccg aagcatttcc cgttgagacc ggccgtcctc cacaatcaga
    13201 atatgtaatt tctgcatggc gctttatcct tggcaatcag gttttgtcgc ttattttttc
    13261 gaccggcagc acaatttcaa acgtggtccc ctcgcccggg cggctgatca cgcgaacgtc
    13321 gccgccgtgg ccgcggataa tctccgtggc cagaggaatg cccagcccca ggcctttttc
    13381 cttggtcgtg tattccgggc tgaaaacttt ttcgatttcc tccggagtca tgccgcagcc
    13441 ggtgtccgca atgctcacca gaacatgttt tttgccctcc cggctgacgg taatggtgat
    13501 tttgcccccg ttcgtgatgg attccatggc atttttgatg aagtttaaaa aggcctgctg
    13561 gagcttgttg atgtccatgg ggatgacggc gggggaaagc cgccactggg tctccagcgt
    13621 aatgtttctt gaggccgcct cttccgccat caggctgaca attttctgca gaacttccgt
    13681 gacggagaaa ttgctcaact ccagccttct gctcttggaa aaagaaagaa actcttcgat
    13741 gatcccgttg agacgccgga tttcatcgcg gatcaccgtg gaaagatttt taaactcccg
    13801 ggcctttgtt tcatccgggg gaatgaaatc tcttttcagg cgctgcgtcg ccatgctgat
    13861 ggcgttgagc ggattgcgta tttcgtgcgc cactcccgcc gccagttgcc ccagcgaaga
    13921 gaggcgctcg gccttttcca gccttcgctc catttcaata atacccgcca gatgccggtt
    13981 ttgatcgtgg tagagcagcc acatggacaa aagagcgatg ccgacgacaa acagcatgaa
    14041 aatgaaaata ttctgacggt tttcggcaat aatcttgtcc atggagccgc gatccagacc
    14101 gatgcggacg attccggcaa ttttttccct ggggaggtag ggattttcca tgacaaaggg
    14161 ggcagccaca tccagaatct tgattccctg ccggtccact ttcctgctca gaattctttt
    14221 cttccccctc atcatctcct gatcgttgag atcctcgctg gtcaagccgg ccggcaattc
    14281 tccgatattc cccagcagaa ttttttgatc attaaagacc tgcaggtaag cgatgccgtg
    14341 gccttcgccc attttgctca tggctctttc gacggcaact ttcaggctcc agtacagtct
    14401 gccttgacgg tccaggcaga agacggcgat tccgctgtct cccttgagtt tcatgccgac
    14461 gtagccgatg tcctgtttga tgcggatttt ttccagcaac tcgaccgttg tagccttgcg
    14521 ccccttgacg gtcaaatcgc ttttcggcag catgtcggtt ttcagaggac cgctctggta
    14581 aatgacgtcg ccccgcatat tgagaacggc cgcataccaa aaatgattat cggccgcaaa
    14641 cttgcgaagg taggcgttgc tcagcaattt ctttttccac tgttcgtcga tttgctgccc
    14701 cagtttggtg atggcatcgt aaaccagctg gatggaatag gcggattccg cctctttggc
    14761 ggttttgaga tgggacggca ttttcccttt ggcggcggca atgcttttca gattatcttc
    14821 cgtctggcgc aaaaggacgc tgatggtggt cagggcctga tcttccataa atccgaccag
    14881 gttggtttcg ttgcggcgga tatccagata gcccatcacc agaatcagcg ctaccaggac
    14941 tgccgacacg atgccgaccg ccagcggttg aagaaaattt ccgttatacg aatgtgcgcc
    15001 gcgttgggtg attgccttct ttttttgaaa aaataacata atatccttat cttacaaaaa
    15061 attaacgggc acttcttcgc tgtgatggtt atttgccggt tatcatcggg ttttttccgg
    15121 gaaaagtcaa cgacaaatga aagcgtaaat cgacattttc gtcgaaaaat aacaaaaaat
    15181 tgcacaaaac ctatctatct gttattgttt gattaaattc attaaaatca aaaccggctc
    15241 gacatttttg ccgaatccgc cggatcgcag gataagggag ctattgggat ttattagcta
    15301 aaatatattt atatcaatta cttatagaaa cactcccgct gtggcacaat atctgctaga
    15361 taatcgttca gaaaaagata agtgtcgact aaaatattac gggcatgatt agccgacagc
    15421 aaaacaacca aggaaggtaa ccagtccttc actcttctct cctttcggga ggctctcaac
    15481 cagcctcccg tttttttatc cttttttctc cgatcccttc tttgcttcgg ttttccggcg
    15541 tcgccactcg gtaattctct ctttgatgat tttttcgtat ccggcatcgg tcggggagta
    15601 aagcactttc ccctgaagct tgtccggaaa atactcctgg ggaacatagg catcggaata
    15661 gtcgtgcgcg tatttgtagt ctttgccgta ctccagctcc ttcatcagtt tggtcggagc
    15721 gttgcggatg tgcaggggaa cgggaagata accggtttta ttaattaaat ctttggtttg
    15781 cccgtatccg gtgtagaggg aattgctttt gggcgccacg gccagataga cggccgcctg
    15841 cgcaatggcc agttcgcctt ccggcaggcc gatgaaatga aacgcctgct gggcggccat
    15901 cgtcagcacc agggcatgag gatcggcgtt gccgatgtct tcggaggcaa agcgcaccat
    15961 gcgccgcaga atgtaaagag ggtcttctcc ggcggccagc atgcggccca gccagtaaag
    16021 cgttgcgtcc ggatcgcttc cccgcatgct tttatgcagc gccgagatca gattgtaatg
    16081 ctcctctccg tccttgtcat acagaagggc ttttttcagc agcgcctcct cggcggattt
    16141 cgccgtgatc gttcgttcct ccccgggcag cgactggacc atcgaggcgg ccgcctccag
    16201 gttgttgagg gccgttcggg cgtcgccgtc ggaaatcccg atgatgaact gtaaggcctc
    16261 ctcggcgatt tggatggcga atttgcccaa gcccctttgc ggatcggaaa gcgcgcgctt
    16321 caggatagcc atcaggtcct cgtccgtaaa ctgcctgagc agcatcacgc gcgttctgga
    16381 caaaagcggg gcgatgactt caaaagaggg attctccgtg gttgcgccga tcagcgtgat
    16441 caatccgctt tccacgtgcg gcaggaaagc gtcctgctgg gctttgttaa agcggtggat
    16501 ttcatcgaca aacaaaatgg ttttctgctt tttggcctcc cagagatccc gggcgtcgtc
    16561 aatcacctgc cggatttcct tgacgccgga aaggacggcg gaaaaactga caaaattggc
    16621 tttggtttca ttcgccagaa tgcgcgccag cgtggtttta ccggagcccg gcggccccca
    16681 gaaaatcatc gaaaaaagct tatcctccgc aatggaccgc ctcaacagcg ttccttcggc
    16741 gaccagatgc ggctggccaa tgtaatcggc aagcgtaagc ggcctcattc tttcggccag
    16801 cggcgccgtg tccaatgcgt catccggagt tccctggctg aataaatcca gatttttcat
    16861 gtttgcctct ttgctttctc cagaatctca atcaaactga ccaccttttc tttgggctgc
    16921 gcgtccgcaa agcgcttctg ctcctcctgc gtcaggtttt cctcaagcag cgacgccacg
    16981 tcaaaagcgg cctgccagca aaaaataatc cgcgcgcagg gcaaatcgcc cgcttcctgc
    17041 aaacagtagg caaacgtcat ttcatcgccc aatttgggac agcgggtgat tttatgatcg
    17101 tgcggggttt tcattgccgg ccagcttggc gaaaaaaaga aaagccgtca agaattttta
    17161 ttttcctgac ggctttccat cagccctgcc tcttcctttt ggcaatcatt tacccataga
    17221 ctatagcctg ttccccataa ccgtagggaa cggtcgcgac catccatgtg ggtggcaaga
    17281 ccgttccctg cgagaatgac atccgttatg gtttgggaaa atccttgatg gcgttcagag
    17341 ctctttcgat ttcgtgatcc ggcagctcgt aggcctccaa tttacccgac agataagcgt
    17401 cgtaggaggc cagatccacc aggccgtgac cgctccagtt gaaaagaatg atcttttcct
    17461 ttccttcctc ccgggcgcgc atggcttcat ccacgacggc ggccagcaca tggttggttt
    17521 ccggcgcggg aatgaagcct tcggaacggg cccatttgac gcccgcatca aacgtcttga
    17581 gctgatcata cgctctgggt tcaaccagtt tttcgcggac cagatggctg acgatgggcg
    17641 ccatgccgtg atagcgaagc ccgcctgcgt ggatcggcgc agggacataa tcgtggccca
    17701 gcgtgtacat cggaagcaac ggcgtggatt tggccagatc cccgaaatca taggcgaacg
    17761 gacctttggt catgctgggg caggaagtgg gctccgctcc gacaattttc acctctttgc
    17821 cgtggatttt gtcgcaggca aacggcgtgg cgatgccggc aaaattgctc ccgccgccgc
    17881 agcatcccat gaccacatcg ggatagaggc cgagtttttt catttgtttc tgcgcttcca
    17941 gaccgatgat cgactggtag aggatcacgt gattgagcac gctgcccaga ccatagtgcg
    18001 tattggggct gttcaccgcg tcttcaatgg cttcgctgat ggcaatgcct aaagaaccgg
    18061 gagtatccgg atattccgcc aaaacattgc ggcccacttg ggtcagattg ctgggactgg
    18121 caatgcattc cgccccccag gtattcatca tcagacggcg gtagggtttc tgctcgtagc
    18181 tgacccggac catgtacacc cggcacgcca gatcaaacat ctggcaggcc atcgccagag
    18241 cgcttcccca ctgaccggcg ccggtttccg tggtcatgcg cttcgttccc gcctcccggt
    18301 tatagtaggc catcggaacc gccgcattgg gtttgtgcga tccggcgggg ctgacgcctt
    18361 catttttata ataaatctgc acggggcagc ccagcagttt ttcgaaattg cgcgcccggt
    18421 aaaggggaga cggacgccac aggacgtatt tttgcaggac ctcgtcggga atgtcaatcc
    18481 agcgctgggc ggaggcttcc tgctcgatga tgttcatggg gaaaatggcc gccagatcct
    18541 ggggcgtgac cggctggccc gttccggggt gcaggggcgg cttcatcggc gtgggcaaat
    18601 ccgccaaaac attgtaccac tgcctgggta tttcatgatc ttccaataac gtcttgacct
    18661 gttccatttc ttctctctcc tttacaaaaa taattcccgg gtttctttca gcaagcgatt
    18721 tttgcgcccg gcccaagccg gcgccaaaaa aaatgccgtg gagctttctt cccacggcat
    18781 ttcaaaatgt ttacgagtca tgggaaggag cctccctgcc catgctcggt gattacagga
    18841 gcctacgggc agacattgat cgcctgccac cgccagtttc tgttaaaaag gcttcttgcc
    18901 atttcttgat cgaccttccg agacccttga aaaatgtcgt ttcgatgagc gagagcgacg
    18961 agaaatccta atgacggccg tttgttcaag atttctccct tcggtcgaaa ggacaaaaac
    19021 ggcagtgatt caaagctctc cttccttcac cgttgacgtt tgcctaacac aaccgcgaaa
    19081 gagatgtcaa gccgaaattt gcagccgacg gtttttttta tccgaaaggg aaagcgccgt
    19141 tgcgccgaag cagacgggct ggggattagc gattcttcgc tccgttgcgc gacagcctga
    19201 cgactggctc ggggctttgc cgggcgcccc cttcaagttg atatttactt ctcgggcaat
    19261 taagagtacg ttaccgcatc agtgctcgtt aggtgcccat aaaacggttc agcgatcaag
    19321 tgagaagcgg catgcagatg acgaaaaata ccgacaggat gtcaggccgg ttaacggtcg
    19381 cgctcctttt cggcgttctg ctcgcgggaa tgacggcgcc gccgtcagcg gcggccgatc
    19441 cacacttcgt catagccgct tcggcccaat cccccccgta ttcctatctg gacaacaaga
    19501 ataacccgca gggaatcctc atcgatttct ggaatttatg ggcgcgcaag accggcgccg
    19561 aagcgtcctt tgccctgacc agcttcgata aggccatcga gctggttcgt gacggcaaag
    19621 cggattttca tgccggcatt ttttattcag agccaagagc gcggcatttg gatttttcga
    19681 cgggctttct cgatgatacg ctgtctctgt ttgttctgga caagctggat gtttactcga
    19741 tgtccgacct gaaaaatgtt cctgtcgtcg ttgggatcag caaagaatat tacgccgtag
    19801 agtacatgaa ggagcattat cccttcatcg gaatccggct ttataacaat aacgaagagt
    19861 tgctccacgc cgcgctccgg cgggagattg ttgctttcgt tgtggactat cccgttgcct
    19921 ttcactatct ttcgaaaaat gattccctgg gccggtatcg ggtgattcag gatatatcca
    19981 gccaaaaatt acgggcagcc gtaaagaaga gcaaccgcgc aacgctcgat tttattaaca
    20041 acggcctcaa aaaaatcagc gacagggagc ttggcgacct cgccgacaaa tggggcctga
    20101 gacagaacag cgtcattccg ccgtggttca agaaagttct gctcggaggc gttgtcgtcg
    20161 ttatcctcct cgcattcttc atccactggg ccctgttgag aaaggaaatc cgcaaacaaa
    20221 cgagcgacct cgccgaaaag aacaaaatcc tcacgctcat gcagcgggat atgatggagg
    20281 tcaacagaac cacacaaaac ctgatggaag acctggaaaa aataagccgc gataacgaac
    20341 agatgctcga tatgattgtc cgcgaaatga aggagaccct ggaacgcatt caacgggcaa
    20401 cggaagacgg agattgcgcc ggtccggctc tgatggaaat tcatgatcaa agcaagaacg
    20461 tcttatccct gattgaaaaa ttccgccagg tttctcccgt cgatgcagaa aactacagcc
    20521 aggaaatgag aaagatcaac ctgacggaat ttttctccaa gaagaagaaa gactttaaaa
    20581 acctttcctt gctgaaaggg atcgatctcc acatggatat gcccgcaaag acggtaaccg
    20641 ccatgatcca tcccggacat tttgacgaac tgtgcaacaa tatttttctc agcaccgtaa
    20701 aatattcccg ggcgggcggg cagatccgca tctctctggc gctggccggg actaccgacg
    20761 aaaaaaagat tgtcattcaa tttggctatc attccctggc cggcggcgaa acgagagagg
    20821 acggttcttc gggggcttcc gaccgggagc tgcctccggt ggaaagcgca tccggaatcc
    20881 ggctggccct gatcaaaaaa ttgatcctgc tccagggagg aaagatatcc gctttcgagc
    20941 gggagcgagg ggaaagaacc atccgtctgg aactgccggc cgtcgaagaa atcgtgaaat
    21001 cctacgagga tattgaagcg ctggcccaca cgggagacat catcctgttt aaagggcttt
    21061 attataaccg acatggtgaa aaatccgtgg caacggattg gacgcatgtc ggactcatcg
    21121 tccggctgcc cggcaacccg ctgcctttga tctgggagtc cacgccgctg gagaatgtgc
    21181 ctgatcaaga gctggggaaa gggaagtccg gcgcgcagct tgtctcgctg aaagagaggc
    21241 tggaaaatta cgaaaccaac gtctatgcga ttcgattcct gaaagtcgtc cgggatccct
    21301 ccatgctcaa ccatctcttc cgttttatct cctatgcgca cacactgccc tttccgggcg
    21361 agcttcgatt ggtcggaaaa gtgattcatg caaaaatatt cagccggctg taccggatga
    21421 aacgtcgtta taagaatatt ttttgttcgg agctggttgc cgaatcctat atggtgatgg
    21481 ggctgcttcc ggaggtcccg cccccttcag cctatatgcc ggttgatttt tcgacgcaaa
    21541 agaaattgcc gttgctcaag ggcgcgcatc tgagcggcga gttcatgatc agctttgaaa
    21601 agtaagcgac cgtgcgggcc agcggcgccg gctcaggcaa cccgtcacgg ttcaaacaaa
    21661 gcgtgacggc ttcgtaaaaa gccaccgctg tcaccgtgag cccgtcgaag ggcgagcgta
    21721 agcgcccgat atcgcgtcat gcttcgacag gctccgcatg acaaaagagg ggaaaaggac
    21781 tttttacgag ggcgtcaagc atgatgaaca tggaaatcgc cgtcatcgtc atctttgcat
    21841 ttttgaccat tgcgatcctg atggcgcggc ctctgatcaa aattcgccga atgtcttccg
    21901 taacgaacag ggcgccggaa ccgcacaagc cgcaactaac gcacaacctt tatcgtcatg
    21961 tgcgggcgct ggcccaccag atcggttcca gaagtctgag cgaatatgat aagctgaagg
    22021 aggcgcaaac ttacattgaa accgttctca gggaatcagg cataccctat tcgattcagg
    22081 attgtccggc acatgaaaaa acctgcgcca acattgtcgt tacttttcca ggcagccgac
    22141 ccctgccgca gaagatcctc atcggagctc actatgacac catccccgga acgccggggg
    22201 cggatgacaa tgcatcttct gtttccgtcc tcctggaaat gtgccgcgcg ctcaaaaact
    22261 ctactccgga caaaaccctt caactcgttt tcttcacgct cgaagaaccg ccgtcttttg
    22321 atacggagcg catgggaagc cgcgtatgcg ccggggaagc caggaagaat aacgaagaca
    22381 ttctgttgat gatttgcctg gagatgatcg gttattacag cccccgcaag aacgggcagg
    22441 aatttcctct gcctttgatg aatctgtttt actccgccgt cccggatttt gtcctggtgg
    22501 ccggcgatct ggcttcccga aaaatggtgg cgttcgccgg cgactgcatt cgtcggacat
    22561 ccggcctgcg cgtcgaaacg ctcacggttc ctcaatgctt tcccggaatc aaactatccg
    22621 accattcctc tttctggcgc cgcgggtatc gggcaatcat gattaccgac accgcgtttt
    22681 accgaaatcc caattatcac acgcccatgg acaccatcga caccctggat ttcaaagcga
    22741 tgacggcgct gtgcgagacg ctgataaaaa tggtccatga actcgacggg cggccggacg
    22801 acggaaattg ccgttgattt cctttccgga gtctgataga ggaccatata atcgaggggt
    22861 gttcataagc tgaaacgact tatcggcgcg agagctttga ataacggcca tttctgtcat
    22921 ttcgacttct tctttgtcat ttcgaccgaa gggagaaatc ttgaacaaac ggccatcctt
    22981 aagattgctc gtcgctcacg ctcctcgaaa tgacattttt caaagctctc ggcacatcca
    23041 tgaacaggag tcgtgatgtg aaagacacgg tcgaaaagcg gcttgagaaa aaagtccaat
    23101 tctacgaaac cgtcctgaac aacatgctga acggcgtcat cattaccgat ctggagggga
    23161 aagtcatatt tttcagcgaa tcctacggac gatttctggg caaggacccc aaagcacaaa
    23221 tcggtttgca cacgacggaa gtcgtggaga attcacgcat gcatattgtt gcaaaaaccg
    23281 gcatcccgga aatcaatcat gctcagcgca tcatggatca cgatatggtg gttcagcgca
    23341 tccccatcaa agatcataac aacaagctgg tggctgtttt cggtcaggtg atgttcgaag
    23401 atgtccggga tgtccaggtc cttgccaaca agctcagcat tctggagtcc aaagtaaaac
    23461 tctatgaaaa ggagctcgtc aatttaagag cgtcgaaata cagttgccgg aacatcatcg
    23521 gcaaaagctc gatcatgacg gagctgcgca aacttgccgt caaagcggcc aagaccaatg
    23581 ccccgattct cctcatggga gaaagcggaa cgggcaaaga actcttcgcc cacgccattc
    23641 atcacgcaag cgaccgtcag gctgcgccgt tcgtcagaat taactgcgcc gcaattccca
    23701 aagatctgat ggaaagcgaa ctcttcggtt atgaacccgg cgcctatacc ggcgccggca
    23761 acaaaagcaa accgggaaaa ttcgaactcg cccatcaggg caccatcttt ctcgacgaaa
    23821 ttaccgaatt gccgctggaa atgcagccca aattactgcg ggtcctggaa gagaaagaat
    23881 tggagaggct ggggggaacg cgcgtcacca aatgtgattt ccggctaatc gccgccaccc
    23941 acgagaacct ggagaaacgg gtgagagaag gacttttccg gcaggacctc tattaccgga
    24001 tcaatgtcat tccaatcaac attcccccct tgaaggaacg gaaggaagat attgcgatga
    24061 tcgcccagca tctcgtcctg acgatgggca aagagtgcgg caccaatatc agcggaatct
    24121 ctcctgaagt catcaggata ttcgaaaaat atgactggcc gggcaatatc cgtgagctct
    24181 ccaatgttct cgaaagaata ttttgctcca ttgagggggt agaggataag atttgcgtgg
    24241 agcatttgcc ggtcttcttc cgcggcctgg ccaagaaacc ctctccatcg acatcgatca
    24301 tcttgaagaa tatcagggaa gattcggaaa aagaagccct gcttcaggcc atccagatca
    24361 gcaacaacaa caagaaccag gctgccaggc tgctcggcat tcatcgcacc gcgctttata
    24421 aaaaaatgaa aaaattcgac attcccctga ttcattccaa gtcctaaaat tctttttttt
    24481 cagctctttt ttctttccaa aagcgtatgc gaatagatac atgtagcgtt acgtctatgg
    24541 ttgcgatcct ttttcactat aatatttcct aagaaaatcc ggtcggttgc cgaattggaa
    24601 cgtcgcttca cgatggcatg cttgttgcga gctcacggga gaatacgatt gcatcaaagg
    24661 gggtgtaaaa agaggaaagg ttgagcagca aagcggccgg ggtggctcca cgacgatcat
    24721 tgacctttag ggaaggaggg aagcatcaga tgattactat tggcgatcaa aagagagatg
    24781 ttatcgtagt tattatggct ctgctctggg cattcgtgct ttatgctgcc tggagcggcg
    24841 ccgtaaataa aaacagcggc ctcattctgt gcggcttttt tgccagcgct ctcatcgttg
    24901 gcgcatttta tttgctcggc gccgttacca atgagaagat gcctgtcggc gttcttgttt
    24961 atccggtgat tttcaacgtt gtctgctggg tgattgcttt tgtcatggcc tatcagactc
    25021 gaggccagaa actggatttt attctgggta tgcatccggg aatgttcggc gcgattgtct
    25081 tcttctggat cggttccatg gttgccgccg ccgtaggcct tctggtttgg tttgataaac
    25141 atatcctgcc tccggataag tgggctgaat tcgaaaatga agtgacgcaa caggaaaaat
    25201 tacactaagg aggacttttc gacatgaacg aacaatttat tcagccttca ccgttgatca
    25261 tcggatatac gatcgttttc atccttatct ctatgggcat cggcttttac gccggcaaat
    25321 acgccaaaac ggccgagcag tttttcggcg gcacgaagat gtttggcggc gtaaccatcg
    25381 ggcttgcctc catggcggcc gtgatgagcg ctttcggttt catcggcggc cccggtctgg
    25441 tttacaaatt cggcatgtcc tccatctgga tgacgatcgc ctgcggtccc ggcatggcat
    25501 acggctactg gatcctggcc aaacggatgc gcggcatggc ggatgtcacc gacgtggcga
    25561 cgctgccgga tatcgcccgg gtgagattca acagccaggc cgttcgcgcc gttctggcca
    25621 tcggattatt cctggccgcc atctcctacc tgtcctctca ggtgatcggc ggcggcatcc
    25681 tgatgaacca gatgctcggg gtctccaatg aatggggcat cgtgatcatg tttggaacga
    25741 ccgtggccta tatgattttc agcggcatgt ccggaagcat tttgaccgac gccttccagg
    25801 gctttgtaat gttgatcgca gtgattgccg tgctcgtcgg ctttttcatg atgaccggcg
    25861 gcgatttaac cccgatcatg aattcgccgc aattcggtcc ttcctttgtg gatggattcg
    25921 gttcgatgcc gcttcaggcc gccctcgtgt tcggactgat cttttttgtg ggcgcgatgg
    25981 gccaacccac gatgctggca aaaatgtatt ccctaaagac gccgcaggat atcaaaaagg
    26041 ccggcgtcat cggagggtta agctacggtg tgacttccat cgtctggatt ctttgcggct
    26101 acggcgcatt gtatatcgtc gcaaccggcg cttctccggc gctggcgaaa gcggacaatg
    26161 ccgccttcct ctttgtaagc aaattgggag gcgtcacgca ggcgctggtg atggcgggcc
    26221 tcttggctgc aatcgtttca acttccagtt tcttcatttc tctgtcttcg agcgccatca
    26281 cccgggatct gatcggcgct ttcggccacg agattcctca cgacaagcag atccgcatcg
    26341 gccagattct gcaggtcatt gttccgctca tcgcgatcgg catggggtta tggggaggaa
    26401 gaatggtggc catcctcggg gcgctggggt gggggttctt tgcttccgtg acgattccca
    26461 ctttcgcgat cggcatgctc tggaagcgtt gctcgacgga aggcattctc accggtttgg
    26521 tgtttgccgt catctcgaat ccggcctatc ttattctcga gaacctgaaa attttcaagt
    26581 ttccttttgc gggctatctt ttctgcatcg ccggagccgt tgtcttgacc gtcgtcgtat
    26641 ccctctttac aaaaacgtcc gcgggagaca atatgcccaa agcgttaaag ccgattttca
    26701 aactgtaaaa acgttgtttt acgtcggggt cgggcacccc cggccccgac gtttttccat
    26761 cgaggattgg ttatgaagaa atttctgtcg atacggctga cggttgcgct gttggcttgc
    26821 gctgtcttct ttgtcgcctg taacggcaac ggatcgaaga aaaaagtcgc ttcggaagat
    26881 caggatttct cctcgtcagg gatgatcaaa gacatggaag aaatgcggaa ggccgcggaa
    26941 aaaggcaagc cgacagcccc gccggcgggg gtcgcgccac aggagtcgcg caaataggct
    27001 tctatcgccg gttgtccggc ggacggcttt atcaagagga ggaatacgat cgatggcgga
    27061 cagcccaact catcattcga aggattcttc acaaaaaaaa gaaaaggtcg tttcagcggc
    27121 cgatgccgtc aagacgatta gggatggcga cgtaattgcc acggaaggtt ttgtcggcgc
    27181 cggttttgcg gaagaactcg ccattgcgct tgcggatcaa tttctgaata ccggcactcc
    27241 ccggaatcta tccattattt acgcggcggg ccagggcgac ggcgccactg ctaaaaaagg
    27301 attgaatcac ctggccctcg aaggacttgt cggcagggtg gttggcggcc acattggact
    27361 tgcgccaaaa ctgcaaacgc tgatcaaaga gaacaagatt cttgcctaca actggcctca
    27421 gggcgtcgtt tcgcattttt ttcgtgaagt ggcggcaaaa cggccggggg tgatctccag
    27481 ggtcggtctg ggaacttata tcgacccgcg atacgatggc ggcaaattaa atgaaaagac
    27541 gttcagggag ggcgcggatc ttgtaaaatt gattgaaatt gaggggcagg aatacctgtt
    27601 ttacaagacc ttccccattc atgtcgcgtt tatccgggga acaacggccg atacgttcgg
    27661 caatatgagc atggaaaagg aagccttgac cctggaggcg ctggcgattg ccatggcggt
    27721 gaaaaacagc ggaggaatcg tgattgcgca agttgagcgc gtcgcagaac aaggcacgct
    27781 gaattgtcgt gacgtgaaag tccccgggat catggtcgat tacgtcgtgg tttcgaggcc
    27841 ggagaatcat tggcagactt ttgccgttca atatgatccc tctctgagcg gagaggtccg
    27901 caaacccatg aaatatatcc cgcctatggc gatggggccg cgaaagatca ttgcgcgacg
    27961 cgccgcgttt gagcttcgtc cgaaaagcgt cgtcaacctg ggcatcggca tgcccgaagg
    28021 agtggccaat gtggcgaacg aagaaggcat ccttacgtcg ctcgtcatga cggcagaacc
    28081 gggcgtgatc ggcggcatgc ccaacggcgg cctgaatttc ggcacgggga caaacacgga
    28141 ctgcctgatc gatcagcctt atcagttcga tttctatgac ggaggcggat tggacgtcgc
    28201 atttctggga tcggcggaaa tcgacaaaca cggcaatatc aatgtcagca aatttggccc
    28261 ccgcttcgtc gggccgggcg gcttcattaa tatcagccag agcaccaagg aaatcgtttt
    28321 tgtcggcact tttacggccg ggggcctgaa agtctccgtt gacgagggga aactgacgat
    28381 tctggaagaa ggcagggaaa gaaaattcct caatcacgtg gaacaggtca ctttcagcgg
    28441 aaagtacgct gcggcagcca agcagccggt tctttacgtg acggaacgtt gcgtgttttc
    28501 tctggtcgag gacggcatag cgttgattga aattgcgccc ggcatagatc tggaaaaaga
    28561 tattttagcg aatatggaat tcaaaccggt gatgaagacg cctccgaaac tgatggacga
    28621 gcgtattttc cagcatggac tcatggggtt gagaaaagag ctggtggtca tcccgctgga
    28681 agaacgtctg atatacaatc ccaagcgaaa ctctttttac gtcaatttct caaactataa
    28741 catacgcacg agcgaagaga tcagaagaat caaaaatggc atcgaagcga tcctcgcgcc
    28801 gctcggaaag aaagtgccca cgatcgtcaa ctacgacaat ttcaatattg ctccggagct
    28861 gacccatgag tatgcggaca tgctcaagca cgtcatgcaa tattacagca atgtgaccag
    28921 atacacgacc agtgcgtttc tgctgatgaa gttgggcgat gaactgctca agcgcgatgt
    28981 ggatctgcag atgtttggca gcatcgaaga cgccaaagaa gctttaaata cactggaaaa
    29041 ataaaatgcc ggccaggaga gagccgttga ataacggcca tttttgtcat ttcgacttct
    29101 tctttgtcat ttcgaccgaa gggagaaatc ttgaacaaac ggccatcctg aagatttctc
    29161 gtcgctcacg ctcctcgaaa tgacattttt caaagctctc caggaaacgc cgacggggga
    29221 atccgtcttt ccaaagcgga gcaaggcgcc gggatcggcc ggatggattc gttatgcgct
    29281 cttttgatca tgtgaagctt gcccgtccac tgcctcccct ccccgcgcag gcaacaacgc
    29341 gtgggggatt gacaatcgcc gcggcttcga ttgcgcacgg cgccgataaa gagtgaagcg
    29401 gcgagagctg agttccgccg ttggcgtcac gcgcggcgcg gcggcggaaa gccttccgct
    29461 gcaaccggcg acgggtctca acctctccgt gagtcaacgc cgataagcgc aagcgggacc
    29521 gccggaacgc cgacccggca aacgttttgc ggatcagcct ttcactcttg gcgacccggc
    29581 ggtttcccct gctttctttc cggaactttc cccgaagcag acgattgaat tcatcattca
    29641 tgtcggtaag acaggcttcg agttttttgg cgttgtgccg ccggtcggca acggctgaat
    29701 atttctgccg cgaaaccggc tcatgacaga gtctctttgc gccttgcgag tcgtttgaca
    29761 ataaaaatca gctatgctac aggaacacgt gtgaccagaa ggtttttgtc atgatacgga
    29821 ttcatgaaac cgcgcagaac tgatttgaat cataaggctt ttcagacagc gcgcctaata
    29881 aacatgaagg agagtgaaac catgacccgg agtgaattca aaaagatcat tgccaacgcc
    29941 atcaaagcgg aagtcgcagc caataaattt tacagcggcg tggccaaaaa agcgaaggac
    30001 gcgaatctca ggaagctgtt ccaggagctt gccggtgaag aagcccagca caagctcacg
    30061 ttaaaaggct atatgaccaa aggggccgcg gaacttcaat tttccgaatc ggcggactac
    30121 agagtcgcgg acgagctccc cacgcctccg ctgacgcccg atctgaaacc ggttgacggt
    30181 ctggtgatcg ccatcaagaa tgaactggca gctatgcaga tgtacaccca attggccaaa
    30241 gcaagcgcgg acaaggcgca gaaaaaggtt ttcctggagc ttgcgtcgat ggagcgcggc
    30301 cacaagaaaa gactcgagga tatatatacg aatatggctt tcccggaagc ctggtagcgc
    30361 tgccatgttt tgatgatttc gtccgggcgc ccggcaggac ccggcaacaa aaaaagtcag
    30421 ggcattgcgg ccctgacttt ttttgttgat caccgccggc gctttaccgg taatatcccg
    30481 ttccgaccac ccagccgaag ggcttgaaca gcagaacata ggatcgcttg ggcagaggcg
    30541 tggcctggcc ctttttggta aacaggtatt cgacatagcc gccggttttg ccccgggcca
    30601 gaaattcctt cacatagaaa acgcctttgg cgtccttgtc ggccagccgg ttgcggcctt
    30661 ccgtatcctt gcggccgtaa agcaccacgt tgacgccttc ggtcgtatcg gcccagaaat
    30721 atccttcctt tccgtagcgc agttcccgca gcagtcctgc ggccagttct tttgcttttt
    30781 ccgcgctcat ctcccctttc tgctgccggg cgtgaatcgc cgccagcagg ctgaccgcgg
    30841 tttccacctg acttttgatc agcgcatccc cggcgccgtc cgaaagaacc ggcgtctgcg
    30901 ccgcaaccga cagacaaaaa acaacgctca gcaaccctcc cagaaccgtc aaccagactt
    30961 tacgcataaa tcctccttca tgaaaataat tttcggttta agacacgacg cttctcttac
    31021 gcctgatttt gaccgctgcg ccggcgcgcc tcgcccatgg cgtttgcagc cagcatggcc
    31081 tgcagaactt tctccggtgt gatgtcaccc gcttcgtgat gaatggactc cccggaagcg
    31141 caagccttac aggctgcctt gtcgatcatc ccgcggtttg cgtccgccag gccgatatcg
    31201 gaaaacgtcg tcggcagccc tattgcttca caaaaatcat agacggtctg gatttcgtcc
    31261 ggcgcggcat ccgtcaggtg cagaccggcg aggacgccga aggcgacttt ttcgccgtga
    31321 taaaaggcgt gggtttgggg aagcgcggtc aggccgttgt gaatggaatg agccgccgcc
    31381 agtcccgagc tttcaaatcc caggccggaa agcagaatgt tcgcctcgat aatgtggttc
    31441 agggccggtg tgacgatatg ttgtgcagcg gccattttcg ccgcacgacc gtaagaaagc
    31501 agcgtatcat aacagagttt ggccagatgc agccccgcca gtgtgctcaa tccgccgcac
    31561 tcattgggcg attgggtgcg gcggcaggac cgggcttcaa accaggtggc cagcgcgtcg
    31621 cccatgccgg atatcaaaaa gcgcgccggc gcttcggcaa tcactcgggt atccaccagc
    31681 acgatttgcg gattcatttt ctgataaaag acggattcga aggtcccctg ctccgaataa
    31741 agaacggcgc agccgctgca gggcgcgtcg gtcgaggcga tggtgggaac aatcatcacc
    31801 ggaaggcccg cgcggtcggc ggcgattttg gcggtgtcga tagccttgcc cccgcccatg
    31861 ccgaccagaa catccacccg ctcgcggcga aggacgtccg ccagccggct caattccttt
    31921 tcgcagcatt ctccggaaaa aggctcgatg agaatcccgc gggtttttaa attctcccgg
    31981 tacggaacca gaattttgtt ttgagcggaa ggagacgcca ggatcaaccc ctttttaccg
    32041 aaagggccaa tcgcgtcggg cagttgcgcg agcacaccct cgccctgaat gtattttccg
    32101 ggaaacaccg cttttaagaa catgagttcg ctcctttgta atgaatgagg ccgccggccg
    32161 acactgtgtt acaccagcgg ctccggaaaa tcaaataatc ctgattgaat accgaaacaa
    32221 attgtgttta gaaacagcag cgacacacgg gaagacacca tgaatcaacc atccagaccg
    32281 gtcaagatcg atttcaattc cgaattccag cgggcatttt cgctcatgga agatacgcag
    32341 aaaaacatcc tgatcaccgg gcgggcgggc acgggaaagt caacgctgct caactatttc
    32401 cgcgatgcca cccaaaagaa agtggtgatt ctggcgccga ccggcgtggc ggcggtcaat
    32461 gtggaaggcc agacgatcca ctcctttttt catttcaaac cgaatgtgac gccggcgtcg
    32521 atcaagcgaa agaaaaaggg ggagaaggac agaccgaccc tttacaagaa actgaccacg
    32581 atcgtgatcg atgaaatttc gatggtgcgc gccgatctgc tggattgcgt cgataaattc
    32641 ctgcgcctca acggccctca tgaaaaacgg cccttcggcg gcgtacagat gatttttatc
    32701 ggcgatctgt atcaactgcc gccggttgtc tcgtcggcgg agcgcgaaat tttcaaaagc
    32761 cattatccga cgccctattt tttcagcgca aaagtctttg atcaactgga catcgagtat
    32821 atcgagctgg aaaaagtcta tcggcagaag gacgatgaat ttgtccggct gctcaacacc
    32881 atccgcaacc gctccgtaac ggatgacgat ctggccaggt ttaatgcgcg ctgcgatccg
    32941 ctcttcgaag cgcctcccgg ctccttttat ctgtctctca ccagcacgaa tgatctggcg
    33001 gacgcaatca atgaaaaacg gctggccgaa ctgccgggga aaatctggag ggccgccggc
    33061 cgaatcgaag gcgatttcgg caaggaatat ctgccgaccg cggtggatct gaagctgaaa
    33121 aaaggcgcac agattatgct gctcaacaac gattcactgg gccagtggat gaacggcacc
    33181 atcggcaaaa ttcgaaggtt cgagcaggag gacgacggcg aggaggtgat tgtggccgaa
    33241 ctggacaacg gagatacggc gcgcatttcc ccctacacct ggaaaatcta ccggtttttc
    33301 ctgaaaaacg aagagctgcg ctcggaggag gtcggatctt tccggcagta tccggtgcgc
    33361 ctggcctttg cggtcaccat tcacaagagc cagggaaaaa cctttgaaaa cgtggtgatc
    33421 gacgtggggc ggggcacctt tgcccacggg cagatgtacg tggcgctgtc gcgctgcacg
    33481 accctcgacg gcattgttct taagaggcct ctccaaaaaa accatatttt aatggactgg
    33541 cgcgtggtca agtttcttac gaatctccag gttgctcaag ccgccaaaac gttcagccgc
    33601 gaggagaaaa taaaatggat tgaagaggcg attgctgaaa agaaaaacat tgaaatcctt
    33661 tacctcaaag gtcaggatga aaaatcgcgc cggacggtga ggcccctgtt catgggaaat
    33721 atggaataca agggttatcc ttacctgggc ctcgaggctt tctgcctgga gcgacgggaa
    33781 aaaagaattt tcaacgtgga caagattctg gaaatcggag aggggaagtc atcggaaaac
    33841 aaaaccggct tctgacagga agattgtgcc aatgcccctt tgcgccacgc cgttatttca
    33901 ggaaaatgga tccgacaatc ggggcgaaat aaaaagcgga cagggccaaa gccacggcgc
    33961 ccgccgcgga aatcagtcgg tcgattcttg ccacgctcgg gtcatggcct tcttttcctc
    34021 ccttgcgtgt tgccgccgca aacgattccc gacgcagcgt gacgctccga tgaccgttca
    34081 taacgccgga caatgcggaa acccctgtga tcgcgcgcat ttttttgctc cctgttttct
    34141 gatccgggtt acatcagata ttttctgacg gaggcggtga tcacgccgcc tattttgagt
    34201 tcttctctgg gacaaatcgc aggataagcc ggattggcgg cctccaggac aacttttccg
    34261 ccctgccggc ggaaatattt catggtccac cggccatcga cttcggccag aacaatgtcg
    34321 ccgcttcggg gcgtccgttc ccgctcgacg atgaccagat cgccttccat gatgcccgcg
    34381 ccgatcatcg agtcccctgt gacccgcagc aaaaaagaag cctcgggcct ggttaccagg
    34441 tactcgtcca tggacagaat atcacgggtg ctttcttcgg ccgacgaggg aatcccggcg
    34501 gccacattgc cgaccagagg aatgccgaag gaaacgcccg acagtttgag aaatccctgc
    34561 gcgtcttttt ccagaatgcc tttttcaatg agcttttcta tccagtaaaa gacgacgctt
    34621 ttggatcgca cctcaaacag gtccatcatt tcggcgtacg tgggcatgcg cttctgatgg
    34681 cggtaaaacg acaccaggct ctgttctacg gattttatgt cgcggatttt tttcatggcc
    34741 cgtcttatat agaacgtacg ttctattgtc aagcattttt ttatttggct gtagagaacg
    34801 gccttgccac ccgcaaggtt tgccgcgatc attccctgct accgggtcat cgtgtatttc
    34861 tgctcgtctt tgggaacgta ccagcggatg aagttgtggc cgatgccgat gggtgccggc
    34921 tcgatgccgc gaacgcggtt ggatatgatg attaaatcgt cgggaacata cagaaaagtg
    34981 taaggctgat cctcggccag aatttcctga aaacggtcgt agtatttttt cctttcgtcc
    35041 tgatggaacg tgctgcggcc cttctccagc atctcgtcgg cttccggatt gctgtaggaa
    35101 acaaaattga gctcttccgg cgcggtcttg cttgagtgcc acacgtcgta ggcgtccggg
    35161 tccggcggga tggtccagcc cagaatgacc gcgtcaaagc gccgtttgtt gatgaaatgg
    35221 gtgacaaacg ccgaccattc cagaacgcgg attttcgcgg caatgccgac ttccgcaagc
    35281 tgccgctgaa taatctccgc gcatttctgg cgggtctcat tgccctgatt ggttagaatt
    35341 tcaaacgcaa aggaccggcc gtccttgtcc agcaaaccgt cgccgtttgc gtccgcccac
    35401 cccgcctgct tcaaaagttc gcgcgctttg gccgggtcgt aggagtagat tttcactttg
    35461 tcgttgtacg cccaggtgcc cggcttgtag ggccccgtcg ccggtttgcc cagtcccagc
    35521 agaaccccgc tgatgatttc gtctttattg atggcgtggg atatggcctg tctcacgcgc
    35581 ctgtccgcaa aaagcgggtt tttcaaatta tagcccagat acgtgtaggc aaaagccaga
    35641 tagcgatatt tgttgaaatt ttccctgaac agattgttct ccgtctgccg cgtgtactgc
    35701 agcggtgtca gccccatcat gccgaggttc tgcgcgcgaa gctccagaaa catggttgcg
    35761 gtgtcgggaa tgatccgcgt aatccggccg tccagatagg gtcgcccctc aaaataatcc
    35821 tcgttggaca ccagaacgat tttctgaccc ggaacccatt ccttgaaccg gtagggcccg
    35881 gtgccgatgg ggtggcgcga aagagggctt tgggtaatgt ctttccccgc gagcaggtga
    35941 gcgggcatca tcgctgcggc ccaactgatt aaagcgggcg cgaaaggctt gtcgtaggta
    36001 acacgaaagg tatagtcgtc cagaatccgg gcttttttga ctttctgaaa atccccggca
    36061 taggcggtgg gcgttttggg atccaccgtc acctgatagg tgaaaagcac gtcggcggcc
    36121 gtgaagggcc ggccgtcatg ccaccgaaca cctttgcgca ggtggaaggt gatcgacaga
    36181 ccatccggcg acacctccca cgattcggcc agatcgccga caatattcat atccttgtcg
    36241 tacttgacca gaccgttgaa aaccatgccg cccacggcgt gagaagccga atcggaagcc
    36301 agaagcggaa tcaggttgct cgcgtccccg atatcgcccc gcaccagaat atcgccatag
    36361 gcgggcggct tgtccccttt ggaaccggcg ttcccggcct gcggcgacct ctcacatgcg
    36421 gcgatcattg tcaccgccag aagcatcatc atgaaattca atatcctgtg catggcggat
    36481 tacataaccc aggaaacccg tctttgcaat atactttgtg tccttttcgc tttccacctc
    36541 cgcatccgtc cgcccttccg ccatatttta tcctcgcttt ttaaagaaat ttgtgaacaa
    36601 gttttgcgcg aaaagagcaa gttgtctctt tcagggagag agttatgaaa gtcaaaacac
    36661 aggagctgtt taatttttat tcacactttg ccggagcggt tgccgccatt atcggaaccg
    36721 tctatctggc cattgccgcc agcgattcgg cctccgggct tgtcaccgcg ctgatttacg
    36781 gaatatccgt cgtttttctt ttttccgcca gcacgctcta ccatgccttt aaaaaggaag
    36841 aaaatgagct gtccttctgg aggaagatgg accggctggc catttttttc atgattgccg
    36901 ggacctttac accgatttgt tatttttgtc tcgacggcgc ctacaagtgg atgatgattg
    36961 cctttcagtg gagtctggtg ggcgtcggac tgatttcaca gatttttttc ccgggggcgc
    37021 cccgaaaact gtatgccgtc atctatttgt tcatgggatg gtcggcgctc ttcaccctca
    37081 agcagatgct ggccaatatg tcctcttccc aggcgattct cctggtgtcg ggcggcgttt
    37141 cctttacgat cggcgccatt atttacgccg tcaaaaagcc ccggatgatt ccgggaattt
    37201 tcagtttcca tgaactcttt cacatcatgg tcctgatcgg cggcatcctt cattacgcca
    37261 tgatctacgg cgtctattcc catccgggcg tttgatcatg agtgcgtctt ccccgcccgc
    37321 accacccggc cctgcaacga atctgcttct ggcggaggaa ctgaaattat ttcttcagga
    37381 ttacgaaaaa aattataagg cgggtgagag caagcctccc gaggtcggga tgcctttcct
    37441 gctcatagca cctgaatccg acaccggcgt cctgctcatt cacggattga tggcggcgcc
    37501 ggcggaagtc cgcgagtggg cggaatttct gcacgccagg ggcctgaccg tttacgcgcc
    37561 ccgattgtcg ggtcacggaa catcctccgg agatctttcg acgcgcaacg ccaacgactg
    37621 gctgtcttcg gttgaccgcg gtcacgccct gctgaaaacc tgctgcacca aaatcctggt
    37681 ggccggtttt tccaccggcg ccggcctcgc gctgcaatcc gccatcctca aacctcgtga
    37741 tttcgaggcg gtgatcgccg tcagcgcacc ccttcggttt aaaagctttt catcgaggtt
    37801 tgccgagctt ctgcacgggt tcaatcttct ctgcgccaat ctgggcatgg atcgcctggc
    37861 cgtgaagttc atgaagaacg acgcggacaa tccccacatt aattatctcc tttgcccggt
    37921 cagctctttc gtgcaggtga aaaagctgat gagaagcgtc ggacggaggc tggccgacat
    37981 tcaaattccg gcgctgatcc tccagggtcg gcaggacccc aaggtcgccc cgaaaagcgg
    38041 gcctgccatc tatgagcgcc tgggcgcgca tcagaagcgc ttcgcctgga ttgagtttaa
    38101 ccggcatggc attgtcctcg gccccatcgc gcgggaagtt tttaaagaag tggagtcttt
    38161 cctgggcgcc tgccgcctga tcgattcccc gaagcagcgc aatgccagga ttggacaggc
    38221 ccaaaagtag tgcggggttt taaccccgcc attcgcggac ataaatgtcc gcactacatt
    38281 tacaaccttc cagcccgatg atggtgcgtg gcgttagcgg cgccgggttt agaggggctg
    38341 aagcaccgca caacaatcat ctctggtgag aacaaagggt tgcaacccct tgttccagtc
    38401 tgctgttcag cattcttttt aatattcatt ttgaccggat gctcattccg atgtaagatg
    38461 cgcccaaaat acgtcggcgt caggcggaaa ttcatggcag aaggggaaaa agaccgggcg
    38521 gctcttcgaa agacgcgcaa agaatcggtg cagatgcggg ccgccggcgc ctgggacatc
    38581 gtgtatgacg ccgtcaacaa tttcagcctg aacggcgacg ccaatcaggc cgcggccgtt
    38641 gcgctctacg ccatcctgtc cgctatcccg ctctttattt taaccctcat tgccgccggg
    38701 tcgttcttca gctccaacac caccctccag acagacatcg tcggcgccgt tcagggattt
    38761 catccctatt tttccgaaac gctcctccag caggtggggc aaatcgaggg caagagcaag
    38821 gtgctgggct ggatcggcgt cctgggcctc gtctggctgt cggccgcgat cttcaacgcc
    38881 gtcgaatcgg cgctgaacat cattttccga tcccggcaca agcgaaatta ttttgtttcc
    38941 aaactgatgg ccatcgccat gatcccggct gcctggatcg tcggcggttt aagcatcctg
    39001 atcacctata tggccacgct gctcgcgaaa gaaccgacgg catcggccga cggccttttg
    39061 ctttccctga ccatgccggc gcaaatcggc ctgcgctaca tcgtccccta cctgatggtc
    39121 gtgattctgg tcgcattcgt ttaccggatc atccccacgg tgaaaatccg tccctccgta
    39181 gccctggccg gaagcgcgat tttcgcgttt ttcgtggaaa tcgccaaaca gctctttacc
    39241 tggtacgtag ccaattacac ccgctataat gtcattttcg gctccctgga aaccgttgtg
    39301 atcctcgtca tctgggtttt ttacgtggcg ctgatcttcc tgttttgcgc ggagatcatg
    39361 tcgtcctttg agcggcgcga catgctgctt ttggaaagag ccttgctcaa acctcatcat
    39421 tcccttctaa aggtggacgc gcgcctcttc cagaaattcg ggcgtattta cgccccaggc
    39481 gaagttgttt ttaacgagaa cgatgccggc agcgagatgt tttatgtgat ttcggggcgc
    39541 gtccgactgg agcgcgtcga cggccgggtc aaaaaaacgc tggcggaaat ggggccgggg
    39601 cagtacttcg gtgaaatggc ggcgctcatt gacgtccgaa ggaccgcaac ggcgcgagcg
    39661 ctcgaaaaca gccacctggc cgtgattgac gaccggacct tccagaacct gattcgtgaa
    39721 agccgggagg tcgcgctggc catgctgcgc gaattcgcct cacgcctgaa aaattccaac
    39781 gcggccctgg aagaattcac gcatcggaga acgcagatgc tcatcctcct gcaaattctc
    39841 gatcagccca aagccaccgt cgatgagcac attgagcaga tttcccggct gacccgaaaa
    39901 gagccgtccc aaatcagaaa catttttcag gagctttccg gacagggaat cgtcaggctc
    39961 cgggacaacc gcccggacat cgaccgcgac aaaatgtggt ccatgtttga ctccggaatc
    40021 tgaaaaaacc gcgtttcggg atgcatcaaa ggaggaatga aaatgatcct gcacccgcga
    40081 acacttcatc gaatcggtcc attcattctt tgcctcgtcc tcctgtgggc gatcaccgca
    40141 caggccgggg aagcgccgcc catccgggtc ggcggcgccg tgcccgccgt cgaaatcgct
    40201 tcgcagtccg aatatctgct cgattactcc gggcgcttgc agtatcagga catcctcagc
    40261 agcggacaac cctttcaacg ccacacgaaa tattcctttc agttcagctt taaaagagca
    40321 acactgtggt ttaaatgccg catcgctccc gccgattccg gggatgaaac ggcgctggcc
    40381 agccgccgct cctttctggt tttcgacaac gccgccctgg gcgccgtcac gctctatgtt
    40441 ccggtcatcc tggacggcaa gccagacctc attgaattcc atggaggatg gcggcaggga
    40501 gagaaaagcc gggaattccc cttcctctac ccgacatttg ttctgcccga caacctggat
    40561 gcgtcccgtc cggtggtcgt ccgggtcgcc accccctatg cgctgcagtt tcgtgcgacg
    40621 ctttatacca tcgacgcttt tcgcaaaatg agctttgtcc tctttctgat cgtcggattt
    40681 ttcgccggca tcctgatcgc catgattctc tacaatctgg ccctgtatct gtttatccgc
    40741 gaccgtcaat atctcttcta catcacctat atcggctttt tgctgctgtg gcagtgcgtt
    40801 ctcgtcggac ttttccagta tatctggccg ccgctgggcg catttctgat gtccggcatc
    40861 ccggtgttcg cagccctgat gatgattttt gccgtttctt tcggcattgt ctttctcaac
    40921 acaccgaaaa cggctccccg ccacgacaaa ctgctcaagg gcctggcggt ttttataggg
    40981 acgatcattg cgctggtttt gctgagacag ttatggcttg gcaatttgct gtcctacctc
    41041 tcgggacagg tggtttccgt cgttcttttc acggccgccc tgtcgtcttt gcgctccggt
    41101 ttccggccgg ccctgttcta cctcatcgcc ttcggtgtct ttctgctgtg cgctttcgtc
    41161 ttctttttca aattttacgg tctgatcccc aataatacct tcaccatgca cattgtcatc
    41221 ttcggcagcg ccgccgaagc tgtccttctg tctttcgcac tgggctaccg tatccgcatt
    41281 atgcaggagg aagaagagca gcttcgcgag cgggaacggg atctcgaggc aatcagcgtc
    41341 acggatgaac tgacggggct tttcaaccgg cgtttcttga atccggcgct ggtgaaaaaa
    41401 acggcggcgg cgcggcgcag cggcacgcga ttatccctgc tgatgctgga tgtcgatcat
    41461 ttcaaggatt tcaacgatac ctacggacat ccggaaggag acaaggtgct ggcggccctg
    41521 ggcgggctgc tgattcagac cctgcgggaa gaagatatcg cttgccgcta tggcggagaa
    41581 gaattcgtcg tcatcctcca caacgccgac ctgaacgccg cgatggaggc cgccgagcgc
    41641 atccgggccg gttttgaaaa aatatccttc cgccccgacc cgaaaaagga gattttcgcg
    41701 accgtcagca tcggcgcagc cgaattgctg ccgcaggaaa cccccgaaca gctccttttc
    41761 cgcgccgatc aggccatgta tcaggccaag caaaccggcc gcaaccggat ttgcagcgcg
    41821 tgaagcgagg ccatggggaa cagtcgcgac tgttccctac agtgaaaaaa taatcaacat
    41881 caaacgacat gcgcttctga gggcacgcac tacccaaaaa aggtcgttgg gaacggtcgc
    41941 gaccgttccc tcctgactga tcactccttg ccggttgcct gctttcctgc catccgccat
    42001 tcgccatcaa cgattcaaat gattgcgtcc tttttctaac ggcttgacac ggaaaccatt
    42061 ctttgaaata tacttctaaa attaaaattt ccggagggca aaatgaaagc agcagtcctg
    42121 cgcggcgcga aggatattcg aaccgaaacg gtccccgatc ccgtctgcgc tccccacggc
    42181 gtcattgtta aagtcaactc ctgcggcgta tgcggctccg accttcactg gtataaagag
    42241 gacgggcacg aaggaaccat tttcggccat gaattcagcg gcgacatcgt ggaagtcggc
    42301 aaaaacgttc aggggatcgc cgtcggcacg cgctgcacgg ccgtcggctt ttctccgtgc
    42361 ggccaatgct tctggtgcaa gcagggaaag gcgcaccgct gctcggccat ggcgcttttg
    42421 ggctatcagt ttcccggcgc gatggctgag tatgtccatg tccccttcgc ggccctggga
    42481 cgcagtgtct ttcccctgcc ggaggaattg acctatgaag atgcggcgtc ggtcgagcct
    42541 ctttccattt cctacttttc cgtcaaccgc ggccagccca aaacatcgga tatcgtggcg
    42601 gtgatcgggc tgggcgtcat tggactttac gccgttcaga tcctcaaggc cctgggcgtg
    42661 gaaaaggttc tggctgccgg acggaggcca tcgcgtctgg ctgcggcaaa acgctgcggc
    42721 gcggatccgg tcatcgacgc agcggccgaa gacacgctgc aggccgtcat gcaggcaacg
    42781 gatcaactgg gcgtcaatac cgtcgtggaa tgcgcgggca accaggccac tttcgatcag
    42841 gccgtagcca tggcgcgcgg cggcggcaag attctgctgg tcggcattta tgaagaagcg
    42901 ctccactggc agcccctttc cgtcatcagc aagaacctga ccctggtggg ctgtctgggc
    42961 ggaaactttc cggccgtgat cgaattgctg aaaagcggca aagcgagcag ccggggaatc
    43021 atctcccatc gttttccctt aagccaggcc gcggaagctt ttcggacgca gcttcaggat
    43081 ccgaccgccg tcaaggtcat ggtgcaaccc tgaaaaaggc agaacatgaa cgaactgtat
    43141 ttagcgcaca cggaatttat cgagcgggac gccccggaga tccgggcttt cgcgcaaaac
    43201 gccgtcggag acgcggcaac gccccgggaa aaggccgtca aactcttctt cgcggttcgc
    43261 gacggaatct actacgaccc ctaccggatc gatccccggc gcgaagcgtt ccgggcgacc
    43321 actgtccttc gccaggggta cggttactgc gtgaccaaag cggtcctgct ggccgctctg
    43381 ctgcggtcgc agggcattgc ctgccggatt catctggccg atgtccgcaa tcacctgacc
    43441 accaaacgcc tcaaagaagt gatgaagacg gacatttttt acaatcacgg ctacaatgac
    43501 gttttgctgg acggccgctg gctgaaagtg acgccgacct tcaacctgtc gctttgtgaa
    43561 aaattcaagg tcaaaccgct cgactgggac ggacaatccg acgcggtgct tcatcccttc
    43621 gacatggagg gacggcggca catggaatac atcaacgacc gcggctcctt tgacgatctg
    43681 ccctttgact tcctcattga aaccttcatc acccgttaca gcggctcgat cgagagcttt
    43741 gcaaatcagg ctgccaaaac gggtcaattt gaagcagagg ccgaagacga ggcccgaaaa
    43801 ctttccgcaa accaaacatg aacgaaatga ctgatttcag tgaactgaaa gccatcctcg
    43861 acgaacgccg ggtttcctcg ggcgaatccg tccgccatct gcattcccac gacgaatcct
    43921 ttcacgcgcc gtctcttccg gacgtcgtcg tgtggcccca taccaccgag gaagcgagcc
    43981 gtctggtccg ctgggccaag ggaaagcggg tgccggtcac ggcctggggc gcaggcacca
    44041 gcctggaagg aaatcccatt cccgtccagg gcggcatggt catggacttc aatgagatga
    44101 accggatcat cgccgtccgt ccggaagact ttcaggcgga tgttcaggcc ggagtcattt
    44161 acaaggaact caacaagacg ctcgcccgcg acggcctttt ctttccgccg gatccggggg
    44221 cggcggccac catcggcggc atgatcggca acaacgccag cggcatccgc tcggtccgct
    44281 acggcgcgac gaaggattac gtcatgcgcc tgaccgtcgt gctgcccgac ggcgaggtca
    44341 ttcatgcggg caaccgcgcg cgcaaaagct cgtcggggta caacctgccc gcactcttta
    44401 ccggatcgga aggaacactg ggcatcatca ccgaagccac gctgaagctc gccggtttgc
    44461 cggtgaattt catggccgtg cgcgcgacct tccccaccgt cctgaacgcc accacgacgg
    44521 tatatcagat catgcgcgca gggcttgcgc ccgccgccat ggaatttctg gacgccaacg
    44581 tcgtgcgcgt gctcaacgcc gaccgggacc tgacgctgga agaaaatccc acgctgctga
    44641 tggaattttc cggatacagc cgccaggggc tggcggaaga aatggccttc gtggaggaaa
    44701 tctgccagca aaacggcgct ctttccatcg accggggaat cggcgctgaa gaaagggcgc
    44761 gcctgtggga aatccggcat cagacctatg aatccatcaa gcgctatcac acgggccttt
    44821 cgcccctgat catggacgtg gcggtgccgc tttcgcgcta cagcgaaatg gtggacttca
    44881 gcaaaaacga ggtgcgggac ctgacggcct acctcttcgg ccatgccggt gacggcaata
    44941 tccatgtcca cgtcatggac gatccgcagg acgcaaaacg ctgggcgcgc gtggaagagg
    45001 ccacccgcag catcgtcatg aaggcgctgg agtttgaagg cacctgcacg ggcgaacacg
    45061 gcatcggcat cggcaaaagc cgttttatgg aaacggagca cggagcaagt ttgatgctca
    45121 tgaagcgcct gaaggcgctg ctcgacccgg aaaacctctt caatcccgga aaattttttc
    45181 tctgatcaaa acccgccgca caggccgtca agggagctcc ccgccatgga agacatcatt
    45241 tacaggggtg tgctgaccaa catcggcgaa ctgtcttttt tagccggaga aaacggcagc
    45301 cggcacctgg aaggcgaaaa aatctggagc gggtattggt cgcactggag cgggaagagc
    45361 gtctgcgccc gcagactgcc gcaaatcgat tatgaaacag gcaaacccgt cattctcctg
    45421 tggccggacg cgcctgttgc cgatgcctcc cttgtcgaac tctacttcaa tgaacggctc
    45481 gtgaaatatc ccttgtcgtt tctggggcat ctggccgttt tggtgaacgg gaaagttttc
    45541 aacttctcgc attggatgaa cgagaacgag gccatgagtc cggaagaata ttttttccgg
    45601 ccggcgctgg gcgagttcgc gccggacccg gcgagcggac gcgacaatac ggaagattcc
    45661 caaaggccct actacgacaa attcggcagg cttttcatgc ggacgattca tgtgctgagg
    45721 atttccggcc tcgacacgcg caggctttcc ggattttttt tcaccgaact cgaaaaaatc
    45781 cgcagcacac cgcccgatcc gaaagagccc ggcaagtacc gggactttca tatcctgacc
    45841 aagagctgcg ccaccattat ccgcgacgga tttcaaagcc tgggattcga gaagatcagc
    45901 ggcatcttcc cccgggatct cttcgtcaac atggcttatt tctttttaaa accgcttcgg
    45961 ctgccaaacg tacaggcttc ccttcatacg ctccggcagc tccaggttcc cgaggccgcc
    46021 cccagcgcca tgccgccgtt gttgaatccg caaaacaggc tccggtacag aaccctcaga
    46081 aaagaatacg acgtcggatg aaatgtttat cccggattac gcaatagcga tatcgtaatg
    46141 gtttgaattt aattttttgt catttcgacc gaagggagaa atcttcaaca aacaaggatg
    46201 atgaagattt ctcttcgtcc cgattcatcg ggactcatcg aaaggaccgc tttgattttt
    46261 ttatatctct cttgcgaaat ccggatttat ttcttttttg caaccggttt gcgcgcaatt
    46321 tttgttctgc ttttgtcccc tttcgccgcc ggtttcgcgc ttccgggctt taccgctttc
    46381 ttctctgcct tttgtttcat cgttttttct ctggccggct ccagcgcaag ccgcagcact
    46441 tccatcatgt cgctcacaaa atggaatttc acctttttcc gcacattggc cggaatatca
    46501 tcgaggtcct tccggttcca cgcgggcaga atgagcgttt tgattcccgc gcgataggcc
    46561 gcgagcacct tttccttgat gccgcccacg ggcagcaccg cgccgcgcag cgtgatctcc
    46621 ccggtcatcg ccagttgctt tctcaccttg cggttggtca gaagcgaggt cagggccgtc
    46681 agcatcgtga cgcccgccga tgggccgtcc ttgggcgtcg cgcccgccgg aacatggatg
    46741 tggatgtcgc ggtcttcgaa aaagtcggga ttcacgccca acgcttcggc gttggtgcgc
    46801 agaaagctca gcgccgccgc gacggactcc ttcatgacgt cgcccaactg accggtcagc
    46861 gtaaggcctt ttttgccctt catggcggtc gcttcgatga agagcatgtc tccgcccacc
    46921 ggcgtccagg ccaggccaat ggcaatgccg ggccgggtaa tgcgcgcatc gatgtcggtg
    46981 ggcacgcgca cggctcccag ataccggtgc acgtcttttt ccgtcacggt tttggacttc
    47041 aggctccctt cggcaatctg cgccgccaca ccgcggcagg cattggcgat ttcgcgctcc
    47101 agattgcgca cacccgcctc ccgggtgtag ccggtgatga tcgtggaaag cgccttgccc
    47161 gtcagtttga actgcgacgc ggtcaggccg ttttccgcaa gctgacgcgg aatgagatat
    47221 ttttcggcga tcttcacctt ctcttcctgc gtgtagccgg tcagttcgat cacttccaga
    47281 cgatccagga gcgccggcgg aatcgtgtcc agcatgttgg ccgtggcaat gaacagaacc
    47341 ttcgacagat caaacggcac atccagataa tgatccgtaa acgtgttgtt ctgctgcggg
    47401 tccagcactt ccagaagcgc cgaagaggga tcgccgcgga agtcgctgcc gagcttgtcg
    47461 atttcatcga gcatgaacac gggattgttc gattccgcgc gcctcaggcc ctgaatgatc
    47521 cggcccggca gagcgccgat gtaggtgcgg cgatggccgc ggatttccgc ctcgtcgcgc
    47581 acgccgccca gcgcgatgcg ccagaacttg cgacccagcg cccgggcgat ggaatgcccc
    47641 agcgaggttt tgccggtgcc cggaggcccc acaaaacaga ggatcgggcc cttggaatcc
    47701 ggcttcagct tgcggacggc cagatactcg ataatccgct ttttcggttt ggacagtccg
    47761 taatgatctt catccaggat tttgcgcgcc gcgacgatat ccagattatc cgccgtggct
    47821 tcatgccacg gcagcgtcgt gatccagtcg agataggtgg aggaaaccgt gtattccgcc
    47881 gaagacggat gcatgcgcga aagcctctcc agttcgcgca gcgcctcttt tttggcttcc
    47941 tcaggcagat ttttctcttc aatttttttg cggtactcgt ccgtctccac cgtattttcg
    48001 tcgctttccc ccagctcctg gcggatggcc ttgagctgct ggcgcaaata atattcccgc
    48061 tggtttttgt cgatgtcgtc cttgaccttc gtctgaatct tattgcccag ctccagcact
    48121 tccatctggt gattcaccat tttggtaagt tctttcaggc gcagcttgac gtcggccgtg
    48181 tccagcactt tctgtttctc ctccaccggc gcattaataa cagacgcaat cagatcggcc
    48241 agcgttcccg cttcgctgat ggacttggcc atcgggccga actccggcgg cagaaacggc
    48301 gaaagcttca gaatccggtc aaacagcgtc agcaggttcg ccatcagcgc ttcggtttcg
    48361 agatccttca cttccttttc atcgaccagc cggatgcgcg cctgctgata cggcttcccc
    48421 tctatgattt cttccacgga gaaacggctg agcccctgta aaagaagctg cgttcggttt
    48481 tccgccgtct tggccatctt cagaatgacc gcgcaggtgc ccatcgcatg caggtcttcc
    48541 ctggcatagg gctggatgtt ttcctgctcc tttttggcca tgatcagccc caccagccgg
    48601 tctttggtca tggcttcatc taccagaacc gacgcgctgc ccgtcacttc caggggaatc
    48661 atcgttttgg gaaacaccat tacattatgc agcggcagga tgggaatcac ttccgggata
    48721 ttgaatgtat ttttattttc agggacgttc tgttccgtca tggtaacctc ctgttgttgt
    48781 cgggcagaaa gcggctaggt ttccgaaatg accgagatcc gatgcgtctt gttgaccggc
    48841 agctttttca tccgcaccat caaaataccg tctgcataag aggcaacgga ggactgcgcg
    48901 tccacgggcg caggcagggc aacgcttctt tcgaaatacc cgtgcggaat ttccgcctgg
    48961 cggtaccggg cattttgcaa aagctgaatg gctttgcgaa tgccggaaat tttcattgta
    49021 agacgattca cttcaatatg cagttcttcc ttgttcagcc cagccatatc ggccagcaca
    49081 atcacttcgt ccgccgattc gcagacatcc acgccgggac gccagatgca ttcataattt
    49141 ttgaaggccg ggcgaaccag atggaaaact tcgtccaccg ctttctgaaa ttcgtcttca
    49201 aaaccaccgc cgaaattaat cttgatgtag gtcatttcct gcgcctctcg gaatcgtttt
    49261 tttgactacc ccgaaagtat agtaattcaa tggggatttg taaaggccgg gatagtggat
    49321 agtgaatagt ggatggtaaa aagcgatgaa cgacgagtgg aaag
//
LOCUS       DAOSRM010000005        46771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_878_length_46771_cov_102.642671, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000005 DAOSRM010000000
VERSION     DAOSRM010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 46771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46771
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_878_length_46771_cov_102.642671"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..568)
                     /locus_tag="PLA58_01270"
     CDS             complement(200..568)
                     /locus_tag="PLA58_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61454.1"
                     /translation="MSEETHKKEHKPHDDQHLDKLTVKELREMAEEFPHEKAIHDMKK
                     EELVAFIKQAKGIKDEGRAHKHKPKGKIRLTRPQVKAKIRELKVLHGRALEDSDREKA
                     ALLRHKISRLKKISRRVAEA"
     gene            811..1362
                     /locus_tag="PLA58_01275"
     CDS             811..1362
                     /locus_tag="PLA58_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOR61455.1"
                     /translation="MNPTGILKEEHQLVLLMLKIMEAVCKKLDLGQAVKTEDLDAIID
                     FIRGFADKCHHAKEEKLLFPALEKAGMPQNDGPIAVMLREHAMGRGFAKSMEEAIGRM
                     KNGNASAAGTFVEKARGYIQLLRNHIDKEDHVLFMMADQRLNPAAQKELLEGFDRVER
                     EETGEGVHEKYHALLHRMRDTYL"
     gene            complement(1624..1938)
                     /locus_tag="PLA58_01280"
     CDS             complement(1624..1938)
                     /locus_tag="PLA58_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61456.1"
                     /translation="MIFGIARFPDPLKNRKKPEVADAREDLRNPPPQDFLLLNLFQPL
                     SRGIEIPDHEIRPVFDGFINRDAAAHVLKEFVVAVFTCAQRLLHLPSLRFELLQLGNP
                     LS"
     gene            2017..>3267
                     /locus_tag="PLA58_01285"
     CDS             2017..>3267
                     /locus_tag="PLA58_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR61457.1"
                     /translation="MAIYDDVTDRKQAECALRESEEKYRALFENAGEAIFVAQDGRVA
                     FLNPMTAQITGYSGEEITSRPFVDFIHADDRDMITDRHTRRLQGEDIPRSYTFRMLRK
                     NGSLLWVQLDTVLINWQGKPATLNFMTDITERKRIEDALRESEHELRALFKSMLNAFV
                     LFESVFDRDGNFVSYRFVYINDAYERITGVKNNEVRGKTVHEVWPNTEASWVKAYGDV
                     ATTGIPSTFDMYHEPTEKLYHCNAYRPWDTKGRFCVVFEDITERRRAEEIIRISEEKF
                     SQAFHSSPILMAISTIEEGRFIDVNETVLRTLLFSREEVIGKTSFELGIFADPVQRQA
                     IRKTTEEKGYARNIEVQMVAKNGQIIEGLFSAEPMTIHNEKCWLTVMVDVTKQKQAER
                     EIQRANALLNSIVENIPNMIFLKDA"
     assembly_gap    3268..3367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3402..>4539
                     /locus_tag="PLA58_01290"
     CDS             3402..>4539
                     /locus_tag="PLA58_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR61458.1"
                     /translation="MIFLKDAGELRFTRFNRAGEELLGHSRDDLLGKNDYDFFPKEQA
                     DFFTQKDREVLRGKVIVDIQEEPLQTRDRGERILHTKKVPILNANGEPEYLMGISEDI
                     TDRKRAEAEKRRLEERLRRAEKMEALGQLAGGVAHDLNNVLGVLSGYSELLLAEIPEG
                     QKARRHVEKILQSTEKGAAIIQDLLTLARRGVLSEEVIQVNDIISGFTRTPAFENIRD
                     YHPGVSFRMDCDPNLLNIKGSPVHLEKTLMNLVSNAAESISGEGEVVIRTENRYLDKA
                     VVGYDEVREGDYVVLSVSDTGTGISDEHREKIFEPFYTKKAMGRSGTGLGLTIVWGTV
                     KDHNGYIDVSSRVGEGTTFTLYFPATRDEQTAPPERTPVERYMGR"
     assembly_gap    4540..4639
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4818..5189)
                     /locus_tag="PLA58_01295"
     CDS             complement(4818..5189)
                     /locus_tag="PLA58_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOR61459.1"
                     /translation="MEFTFAHNNFNVLNLEKSLAFYREALGLKEVRRYEPPDGSFVLV
                     YLGDGRTPHQLELTWMRERTDPYNLGDNEFHLAFRVDDFAAAHALHEKMGCICFENKA
                     MGIYFISDPDDYWLEIVPAKR"
     gene            complement(5241..6398)
                     /locus_tag="PLA58_01300"
     CDS             complement(5241..6398)
                     /locus_tag="PLA58_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR61460.1"
                     /translation="MNNFTFYNPTKILFGEGRIKDIAGEIPPRAKILMTYGGGSIKRN
                     GVYDEVRAALQSCAMEEFGGIEPNPTYETLMRCVETARKNKIDFLLAVGGGSVIDGTK
                     FIAAAVPHEGDPWEIVASYGKKAKGAIPFGTVLTIPATGSEMNNGAVITNRALNAKLP
                     FMHPSLFPKFSVLDPTKTYTLPPEQIGNGVVDTFVHVTEQYLTFPVNGKLQDRFAEGL
                     LLTLIEEGPRALAEPENYDVRANLMWCATLGLNGLIGVGVPQDWATHMIGHELTALRG
                     LDHGKSLAVVLPALLKMRAGEKKGKLLQYAERVWGITTGEDTARIDAAIEKTRDFFES
                     MKVPTRLCAYHIGADVIPDVVENLKAHGMVKLGENRDISPELVELMMKDCL"
     gene            complement(6395..6955)
                     /locus_tag="PLA58_01305"
     CDS             complement(6395..6955)
                     /locus_tag="PLA58_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOR61461.1"
                     /translation="MGKAIVDRLKKEYPIKEEWVSYELHPETPPGGLLLSERFKGRDL
                     APFYAGLKSRGRELGIVFNEHTVLSNSRLALLASEYARDQGRHDVFHENMFRAYLSRR
                     QDIGRPEVVGAVAAESGLDEQEALAAAKDGRYAPRLAQAAKEGRLLGLTGIPLFIVNN
                     QYQITGAQPLESFRRLLQSISQKEGI"
     gene            complement(7073..8884)
                     /locus_tag="PLA58_01310"
     CDS             complement(7073..8884)
                     /locus_tag="PLA58_01310"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOR61462.1"
                     /translation="MNDSGMTKNKKLVQWVDDMAKMCKPEKIYWCDGSRNEYDSMIRI
                     MVDSGLATPLNKEKRPNCFLFRSDPSDVARVEDRTYIASKSRDEAGPTNNWIDPDELK
                     KTMKGLYDGCMKGRTMYVIPFSMGPVGSPIARIGIEITDSPYVVVNMHIMTRVGQKVL
                     DVLGEDGVFIPCLHSVGKPLAEGESDGSKWPCAPIERKYISHFPEEKTIWSYGSGYGG
                     NALLGKKCFALRIASVMAREQGWLAEHMLILGITNPEGEKRYVAAAFPSACGKTNLAM
                     LIPTIPGWKVETVGDDIAWMKFGADGRLYAINPEAGFFGVAPGTSEDSNYNAMKAIEK
                     NTIFTNVALTEDNDVWWEGIGYDAPAGRIIDWTGNVWTKDSGKVAAHANSRFTAPARQ
                     CPAIDPDWENPKGVPISAFLFGGRRPSTVPLVHQSFNWNHGVFLGSIVGSEITAAAIS
                     ANIGQVRRDPFAMLPFCGYNMGDYFQHWINIGTKTSADKLPKIFYVNWFRKTADGKWL
                     WPGYGENSRVLKWVFERSAGKGKASETAIGFMPTEDAIDTTGLSVSAADMKELLSVNK
                     EEWKKEIEGIREHYKKFGDRLPAELKKELNALEKRLS"
     gene            complement(9191..10123)
                     /locus_tag="PLA58_01315"
     CDS             complement(9191..10123)
                     /locus_tag="PLA58_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61463.1"
                     /translation="MIMQTPFRLHDFILVVVVILSMTVAILFPDFGARFQALPVYCLM
                     INFFLSCLSIDLADVWKALRGHIREILGFTALKLALLPVVLYGVFYAVAPDYALAALL
                     LTGVSTGVVSPMISNMVKGNSSLVLVVVVITSALAPFTLPALIKIVIAKDAAISFLAM
                     FKMLAMVIFIPILIVEIIRYLLPRLVAPILKIQFPVALLMFALINLGVFYRWAPFFKE
                     EPSAILMATVVVFVLAAIYCAVGIFFFRKGPLHNHLAGAVMLGNINNVLVIVFSSEFF
                     GPVEPLVAAIYMIPFFVIVIPLRYYRYRKTRHLK"
     gene            complement(10165..>10604)
                     /locus_tag="PLA58_01320"
     CDS             complement(10165..>10604)
                     /locus_tag="PLA58_01320"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOR61464.1"
                     /translation="KCRAAVYYPAEGNPMYSLLADALIIIHFLFILFVVTGGLLILRW
                     PQMVWLHLPAACWGAFIEFAGWICPLTPLENHLRRLGGEAPYTGDFVVQYLIPVIYPA
                     GLSALTQYILGGLVIAVNLLIYGYVLYRRFSRKVPSSMTDIKL"
     assembly_gap    10605..10704
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10941..11795
                     /locus_tag="PLA58_01325"
     CDS             10941..11795
                     /locus_tag="PLA58_01325"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOR61465.1"
                     /translation="MAKKRRRKILEAPFPFEWEAIIRKNMAHYKLLDEDEQRRLRERI
                     AVFMAEKNWEGCGGLELTDEIRVTISAMACLLILNLPRQDYENVESILVYPSERILPE
                     QKIGFFETALAPLETPGPISGQAFEQGPILLVWDAVHESIRYAGYGYNVVYHEFAHKL
                     DMQDGVADGTPRLRDRAEYRDWVKTCSREYKKLLKNVERGKRTFLDEYGAKDEAEFFA
                     VATEHFFDQPLGMKRAVPELYRVLKNFYRQDPAARESQYAAAREEQTRKTEAAFAKAT
                     DEKKGDRE"
     gene            11792..12139
                     /locus_tag="PLA58_01330"
     CDS             11792..12139
                     /locus_tag="PLA58_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61466.1"
                     /translation="MIDYIERHETYVRELLTGHPEKETLAELLVYHDRQISWVQQERL
                     AHLITMMFVCLFFLLAFGWTLIHFTLPYILLTALLLILSAAYILHYYRLENSVQRWYV
                     LSNQIREKLLQAK"
     gene            complement(12229..13872)
                     /gene="groL"
                     /locus_tag="PLA58_01335"
     CDS             complement(12229..13872)
                     /gene="groL"
                     /locus_tag="PLA58_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417396.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOR61467.1"
                     /translation="MGAKILLYDEEARKSVLKGVNTLADAVKVTLGPKGRNVIIDKSF
                     GSPTVTKDGVTVAKEIELEDKFENMGAQMVKEVASKTSDVAGDGTTTATILAQAIYRE
                     GVKAVAAGSNPMDVKRGIDKAVETVVKELGKMSKPTKDQKEIAQVGTISANNDETIGN
                     IIAGAMDKVGKEGVITVEEAKGLETELEIVEGMQFDRGYLSPYFVTNPDKMIVALEDA
                     LILIYEKKISGMKDLLPILEQIAKMGRPLLIIAEDIEGEALATLVVNKIRGTLHVAAV
                     KAPGFGDRRKAMLEDIAILTGGKMISEDMGYKLENVKIEDLGRAKKITIDKDNTTIID
                     GAGKRAELEGRVKQIRAQIEETTSDYDREKLQERLAKLVGGVAVIKVGAATETEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVAYLRTLPALVKLALDGDEQIGVNIVKKAIEEPI
                     KMIACNAGLEGSIVVEKVKEKKGSYGFNARTDEYEDMIAAGVIDPTKVTRFALQNAAS
                     VAALLLTTQCMIADKPEEKGAGGGMPGMPPGGGYPGMGM"
     gene            complement(13898..14188)
                     /gene="groES"
                     /locus_tag="PLA58_01340"
     CDS             complement(13898..14188)
                     /gene="groES"
                     /locus_tag="PLA58_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOR61468.1"
                     /translation="MKIRPLQDRIIVKRLEEETKTKGGIIIPDTAKEKPIEGKVIAVG
                     KGKVADDGKLIKPEVTVGDKVLFSKYGGTEVKIDGEEYLIMREDDILGIIEK"
     gene            complement(14320..15678)
                     /gene="radA"
                     /locus_tag="PLA58_01345"
     CDS             complement(14320..15678)
                     /gene="radA"
                     /locus_tag="PLA58_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOR61469.1"
                     /translation="MKKSKTSFFCQHCGYMSPKWLGKCPSCNGWNCFAEELVTDPEAG
                     GRPDLVFGGKPLPIFDIPAEEGKRIVTGMAEIDRVLGGGIVSGSAILIGGEPGIGKST
                     LMLQMMKNLAAGGRKVLYVSGEESASQIRLRADRIGAQARDLLVLVEVSLEKILEQIR
                     KIEPAVVVIDSVQTVYSGDLMSAPGSVGQVREASSRLILAAKKSAVPLFLVGHVTKDG
                     AIAGPKVLEHMVDTVLYFEGDSGHAYRIVRSMKNRFGPANEIGVFEMRDRGLTEVPNP
                     SAFFLAERPENAPGSVVLASLEGTRPILVEIQALVSASNLGMPRRTAIGVDHNRVSLL
                     VAVLDKICGLNLGGSDIFLNVAGGVRVEEPAVDLAVVSAMASSFLDRPVDARTVVLGE
                     VGLTGEVRAISQIDIRVKEAARLGFNRCIVPKTNIASLTQTAKMEIRTITSLKELLEI
                     LF"
     gene            15761..18046
                     /locus_tag="PLA58_01350"
     CDS             15761..18046
                     /locus_tag="PLA58_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOR61470.1"
                     /translation="MIRIRILILSAIPVFVFSLPVFGFFDDIRLSSYAQKQQIHEIIA
                     MLQPKLDKGDTLNPLQIYLLAASYTSTRDYRKALMTIDIMQKQIDRGERTYLGSDFTV
                     YPQILRGGIYLGQGDFLKSIAEGDLAYRLLHENGREKQNFYTSQLIEIYDFLGVAHAL
                     AGHQTEAQKIAGALQKVSITVMNGPPKYSALARIYMALKEYDKALTAIRNPSARVTGF
                     ITAFYDQTFQEIPKLYILSKSLYETSNLPEARQGYDQLLRHPQIGQIGGIYWIVLCDR
                     AKIALSEGQKSMAEDLLKKAVQVIEQQRSSINTEAGRIGFVGDKQDAYAQLIDLLLSD
                     KRYPEAFAYVERSKARALVDLLSSQKNIPVRGENVQRLKMNLNQMAEAESELAAVSSA
                     ANRESQNKTRSVLLTLKKELGEKAPETAALVTVQAPSTTDIQKLIGSDETLLEYYSTG
                     RNWYVFVLSPSSVAVYKLTTDDLAKDVHDFRKMITLNASESYKARAEALYRQLFAFVE
                     PSIKTRKLIIVPHGALHYLPFCALAQGGNYLIDRYSIRTLPSASVLSFLKARTGQPRT
                     GRSLILGNPKLDDPKYDLQFAQAEALAVGAILPNSKVLLRSEATKTNFMAESSQFSII
                     HLAVHGTFDPDKPLDSSLLLSGDKVSDGLLTSADLYRLSLHADLVVLSACETALGKVA
                     SGDDVIGFTRGFLYAGAGSLISSLWQVDDQATHDLMVDFYSNRKTMPKDESLRQAQIK
                     VKQKYPHPFYWAAFLLTGNAL"
     gene            complement(18380..19834)
                     /gene="gltX"
                     /locus_tag="PLA58_01355"
     CDS             complement(18380..19834)
                     /gene="gltX"
                     /locus_tag="PLA58_01355"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOR61471.1"
                     /translation="MEHKARVRFAPSPTGDLHVGNARTALFNWMFARREGGCFILRIE
                     DTDESRSDSAYQTGLLEDLKWLGLDWDEGPDIGGAYGPYRQSERLDLYAAHLKKLMDA
                     DLAYPCYCTEEELEEERRTLILARRMPRYSGKCRNLSEKEREALAASGRKPSYRFKVP
                     RTTIEFHDLIRGPMRFEGEAMGDFIIVRSNGMPAYNFAVVIDDHLMAISHVIRGEDHL
                     SNTALQILLYRAFGFDEPVFAHHCLILGKDHAKLGKRHGSVSVGEFRKQGFLPEALLN
                     YLGLLGSSFAGGREILSRDEMIGAFSLFRASKSGALFDEDKLRWLNALYIRNLTAGDL
                     LERLQPFLREAGYQPETLDPLWLKEIIGIVKEELTTLAEIGDHIDLFFDERYSPSQEA
                     RRLLAAPGAREVVGAFGAYLNRAQGDAAEIYAAAIRHAKEKSGARGKDLFMPVRAALT
                     GKIKGPELDKVFVILGKESAVKRLKRAEQEIIKA"
     gene            complement(19824..21014)
                     /gene="ispD"
                     /locus_tag="PLA58_01360"
     CDS             complement(19824..21014)
                     /gene="ispD"
                     /locus_tag="PLA58_01360"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOR61472.1"
                     /translation="MKTVAIIPAGGSGKRVGAPMAKQYLLLHALPVLVRTLLVFQKVE
                     AISDIIVVVPGDDVLSVRKQVVEKYNLTKVAAVVAGGRQRQDSVACGLRAVKLPCDIV
                     MVHDAVRPFVTEDMIIRVLAAAAGCGAASLGIPAKDTIKETTEDGIVKTTLPRRNLWQ
                     TQTPQAFSYNLLRRAYEAAEKDHYYGTDDASLAERIGAKVQMITGSDENMKITTPEDV
                     KMAESLLKAAAARQRRPRAGLGYDSHRFAKDRKLKLGGVEIPFDRGLAGHSDADALLH
                     AVCDALLGMAGAGDIGRHFPDNDPAFKDISSLVLLERVGRIVESRGVSIENIDATVVL
                     EKPRLAPYAQAMAANIARALKIPETAVNIKAKTNEEMGFAGRGEGIAVWATACGTEGI
                     ADGA"
     gene            complement(21233..22261)
                     /locus_tag="PLA58_01365"
     CDS             complement(21233..22261)
                     /locus_tag="PLA58_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOR61473.1"
                     /translation="MKRLIVRALLVLICSVSGYFIAYYAMAAASPYAVFQAIFGLIIG
                     LVVALLVIRVERNIRKQSVRIIAGGVVGMIIGLLIALMIGFGLNMVIRIPQNQQVVPW
                     IYLLLIGILGYLGLVIGSKKAEEVNFRTPDSRKLMDHRLLDTSVIIDGRIADICDTGF
                     VDGELIVPRFVLNELQFIADSSDSMKRSRGRRGLDVLNRMQKSTAINIEIVEQDFPKI
                     KGVDGKLVALAQKLNAKLLTNDYNLNKVAELQGVRVLNVNELANAMKPVVLPGEQMTV
                     KIIREGKEQGQGVGYLDDGTMIVVDSAQKMINTTVDVVVTSVLQTTAGRMIFTELKEA
                     ADKKSGNA"
     gene            complement(22437..22922)
                     /locus_tag="PLA58_01370"
     CDS             complement(22437..22922)
                     /locus_tag="PLA58_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOR61474.1"
                     /translation="MFKVGDLAVYPAQGVGVIEAIENREVMGKKQPFYVMKIMGNGMK
                     IMIPTNSAKAVGLREVILEKEIPKVYEILRNKDVTIDKQTWNKRYREYLEKIKTGSVF
                     EIASVLRDLFILKSDKNLSFGERKMMDTAKNLLVKEISVATNAQEAKVERDLQMIFTV
                     Q"
     gene            complement(23042..23980)
                     /locus_tag="PLA58_01375"
     CDS             complement(23042..23980)
                     /locus_tag="PLA58_01375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR61475.1"
                     /translation="MERMIRSAFARRFLAVIRGRYFGACFLLCIAALLLPVGCANSQM
                     QKTQASGVYHIVKKGETLYSIARAYSTGLQELAEINNIEDVASIREGSVIFIPNADQV
                     IEDVMVSAGKAAADAGKEEKVKDIKPSGGEKPSTLAKPLPSQEKTSQTPAGTAKPSRP
                     DTGTVLPAPKADIPGREKPQVSEEKVVMEKGRFAWPFRGEVKTRFGIQPNKTYHNWIK
                     IACPAGTRVKAAASGTVIFSANLKDFGETVIIRHANDFATVYTHLSKRSVRTDQDVGK
                     GQTIGHAGKTDEAGETYFNFEIRIKGKARNPLFYLP"
     gene            complement(23968..24621)
                     /locus_tag="PLA58_01380"
     CDS             complement(23968..24621)
                     /locus_tag="PLA58_01380"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOR61476.1"
                     /translation="MDRYKKQRIRMVETQIRGRSIRDDRVLKVMETVPRHLFVDEGLI
                     NQAYSDNPLPIGERQTISQPYIVALMTQALELKGMERVLEIGTGSGYQTAILAKLADR
                     VFSIERVAVLASRARKILDRLNCYNVAIRVGDGSYGWKEEAPFDAIITTAAAPQFPQH
                     LVAQLSPGGRMVVPVGGREVQTLYKLTRSTEDPSELKKEDLGGCRFVSLIGESGWKE"
     gene            complement(24621..25016)
                     /locus_tag="PLA58_01385"
     CDS             complement(24621..25016)
                     /locus_tag="PLA58_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61477.1"
                     /translation="MAQALHSGREKHQESLCEELLRERAAVLARAGFAVEDALAKLDR
                     LDRRFGEMMFRWQSLQTGSPEGAHPKEKQSVFEEINEIVEQFNAACQKAEIQYYYLIV
                     TREALGLRRHETVQRLYRIPSKKKKMQAV"
     gene            25179..26765
                     /locus_tag="PLA58_01390"
     CDS             25179..26765
                     /locus_tag="PLA58_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOR61478.1"
                     /translation="MRSETTFPMSRAVLNAYAEGQEDETLLPLVGVNQQQTPLGRIQP
                     GDSIIFYNIRGEREIELSRSFTEENFRKFPVHPGLPVHFTTMIEYSKDLNTGVAFPPR
                     GVLSDTLSDIIAAHGLAQAKITEAEKAVHISYFFNGKKSDPLPKEERIVVPTRKDVRL
                     FDEAPEMSIDEISREAEARIQDPSRRFIVVNFPNVDVVGHIENEPAILRAVEAVDRQT
                     GALVDQALRQNMAVIVTADHGTVEKWLYPDGGVDTGHTDSPVPFILLHPEQKFSLKSE
                     GELTDIAPTILDLMGLPVPASMTGSSLIQKKPSPVVPVSRLLLLILDGWGLCENTKGN
                     LIACAQTPVMDRLLRDCPHTRLAASGPAVGLPAGTVGNSEAGHLHIGAGRRIYSDRLL
                     IDQAIARKEFHRNPAFLRAMENARQNNRALHLMGIVSFFSSHGSIEHLLALMEMAKQQ
                     GVSELYIHAMLGRRGEQAESGARYIRQVEEKCRSLGLGQVVSVIGRYWSMDREENWDR
                     IEKTYRMLVYGEGTPVPTEG"
     gene            26768..27301
                     /locus_tag="PLA58_01395"
     CDS             26768..27301
                     /locus_tag="PLA58_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOR61479.1"
                     /translation="MKTTVNQTVIELVCGDITQEETDAIVNAANSRLAGGAGVDGAIH
                     RAGGPAIMAECRKIGGCPTGEAVITTGGNLKARFVIHTVGPVYRGGGKGEAGLLASAY
                     QNSLNLARKKGLASLSFPAISCGVYGYPLREAASLALSTCVAFAKNHPEIRHIRHVLF
                     DQRTYDIFAQELKKITP"
     gene            27315..29309
                     /locus_tag="PLA58_01400"
     CDS             27315..29309
                     /locus_tag="PLA58_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR61480.1"
                     /translation="MNAKPTYEELEQKVALLSRKVNEQQAIENQYRNLVDSTSDSLYL
                     VDADGRYLFINHNHARRLKIQQNDLAGLSYKDIHSPEQNAKFERKIQGVYQTGLSDSD
                     EHHSLRDGRYFLRTFSPVRDSTHPERVIAVAVVSKDITPRREAEEALRRSEEKYRRLI
                     EYSSEAIFIISGGKISFANNRTEKMLGYPLADLQAMPFLELVIAEDRDMIDERQQAVL
                     QESEPSLNNRFRMVRRDESTIWVAANSVLMRWENAPATLNFMRDITRQKKAEAKLLQA
                     QKMESIGTLAGGIAHDFNNLLMGIQGHASLALLNLHPGDPNYEHIRTIETLVVSGAGL
                     TRQLLGFARGGKHEVKSVDLNDLIHQTSRTMGRTKKEINIHHHFEDPLWMTEIDPAQI
                     EQVLVNLFINAWQAMPVGGDLHLETKNMIVDENRFKDFSGKGGRYVKISVTDTGVGMD
                     EKTRERIFEPFFTTREMGRGTGLGLASVYGIIKNHNGFINVYSEKGQGSTFNIYLPAS
                     AHQSQNHQCPEEASAPERSSDRETILVVDDEAMVLDVAGEMLRTLNYRVITVPNGAAA
                     IDLYRREQERVDLVILDMVMPDMNGSEVFDAIRRINPGVRVLLSSGYSLNGQAGKIME
                     RGCSGFIQKPFSISELAGKLRTILEQPSPRKKAPASGSMS"
     gene            29364..29858
                     /locus_tag="PLA58_01405"
     CDS             29364..29858
                     /locus_tag="PLA58_01405"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOR61481.1"
                     /translation="MRKHFKGHLQPVGDILLAALKKRGLAGKLEENFLFKLWPKAVGA
                     KIASQTCPDGWRAGTLFVKTTSSVWVQQLHFMKEDIRKKINDLAGKTVVREIHFTVGY
                     SPARKGAGRRAVPPKLMLKERDKRIIAECTETLKDRELAEIVQRVMQAEISRRRLREE
                     RQGR"
     gene            complement(29818..30573)
                     /locus_tag="PLA58_01410"
     CDS             complement(29818..30573)
                     /locus_tag="PLA58_01410"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOR61482.1"
                     /translation="MTDEETVDEILDGSLKIRQSARGYRFTLDSLMLAGFASVKPRSV
                     NLDLGCGNGILALVMARRHPLTRWIGLEIQEGLAKLACRNVEQNGLEKRVAIVAGDAR
                     DVGRAFPSHSFDNVLFNPPYRKMSSGRMNPLSEKAIARHELQGSLADFLAASKVLLKP
                     KGRVFTIYPATRLVELVSLFRRTGIEPKRMKLVFSDASSDAAFVLAEGRNGSREELKM
                     EPPLYIYDAQKTYTPEMKKLFSDLAAPPEACGG"
     gene            30936..33134
                     /locus_tag="PLA58_01415"
     CDS             30936..33134
                     /locus_tag="PLA58_01415"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOR61483.1"
                     /translation="MPKLSLKRKIIGLAVVAAVLPVVVMMILMGSFKTSVSNLASKEL
                     GDVAMANMSQIARDVYGLCETANDLMQRKINNDLNVARSILKQQGGIDAAREKVAWDA
                     VNQLTQETQKVELPKLTAGGVWLGQNRNPSTPTPVVDDVKRLVGITCTIFQRMNEGGD
                     MLRVATNVEDLNHRRAIGTYIPNLLPDGKVNPVTAAVLKGQPYRGLAYVVNTWYLTAY
                     EPITDRSGKIIGMLYVGEKLEAVSSLRKAIMNMKVGKLGYVGVVGGKNEHRGRYVISK
                     DGKRDGENIWDQKDVHGNLTIQTLITKALAQPKGEAFYHEYIWQNPGDPGPRKKISSV
                     IYFEPFDWVIVAGTYEDDFFGPIGHVHDMIRSIFLKMILAGALIILLSVLLAVVLARR
                     LTAPLELTTSLAQKIATGDIQQTREELAGAESTRIIQSGDETGKLLQAFQAMTGNLDS
                     LIGQVHRSGIQVTTSATEIAASARELEATVAEQAASTTEVTATSREIAATADDLAQTM
                     DGVNASLNETIDMAQEGRKDLSRMETAMRELVKATSSITSKLTVINDRANRISHVVTT
                     INKISDQTNLLSLNAAIEAEKAGEFGRGFSVVAREISRLADQTAIATQDIQQVVSEMQ
                     TSVASGVMEMDKFNDEVRRGVETMAAQSGQLAQIIDQVRIHGPQFTAVKDGMYGQSQG
                     AQQISEAMGQLSLAAEQTKASLHEFKLATEQLNEAIAGLQSEVSRFKISS"
     gene            33149..33613
                     /locus_tag="PLA58_01420"
     CDS             33149..33613
                     /locus_tag="PLA58_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOR61484.1"
                     /translation="MLLLQFQAGTDRYGLDIGQIIEVSSMVSLRAVPHAPPEVAGTFN
                     YRGTLTPVVDLTVLLTGASSRPRMSTRIILIAYQGEDGRSHILGLLAEAVTEMIDCRK
                     ESLQQPVVTVSGAPYLGDTMFDERGSIQVLDMERLLTRDLLFTLFPPEVANG"
     gene            33610..34929
                     /locus_tag="PLA58_01425"
     CDS             33610..34929
                     /locus_tag="PLA58_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOR61485.1"
                     /translation="MNGPSAIMEFLTLSLGMEPDSAGKDAVNRAVAAAMRHEGVSDPR
                     AFEKLFGSSPAVRQRLIDAVVVGETWFFRDRGPFLCLARHALNWHEAHPGNVLNILSA
                     PCATGEEPYSIVMTLLAAGLPPSSFTVDGVDVSALALEKARLACYSKSAFRGNIGPDV
                     ARFFETTPSGRKVADPVVRRVAFSLDNLALPGSLAGRGPYAIIFCRNLLIYMTPEARL
                     RIFDRMDRLLIPGGLLFTGHTETNFWHQRGYLPLPWDRAFALTKPASSASFAAVHTGT
                     PAKPISPKGKPGKIAVLPTDAAAKGKTPIHPDTDPPETPSGKRRQPAKPGAANPDVKF
                     PPTHAQIQEARRLADAGDTAGAARLCREYTRKFGPAAETYCLMGILSMAGQDLNGAEE
                     CFLKALYLDPNHYESLVHISLIYRQKGKEIQAALYRERAERKATVKE"
     gene            34958..35620
                     /locus_tag="PLA58_01430"
     CDS             34958..35620
                     /locus_tag="PLA58_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOR61486.1"
                     /translation="MANAEKYCWKVKGIYGDGSCPELARLIHCRRCPVFATAGRQHLD
                     RDLPLTYLRERAAVMALPKETGSLGTLSVMVFRLCGEKFAVKTIYFQEAAEPSPVHTL
                     PLKVNRIFRGIANINGELLLCLSVADLLDLTSEAQESRPAPAYERMLVVGREGQRFVF
                     PVEQILGVHRIAPEDLGEVPATLSRSARALTRGIFLLNGRPVGLLDEERLFPAMTRSL
                     NP"
     gene            35617..37992
                     /locus_tag="PLA58_01435"
     CDS             35617..37992
                     /locus_tag="PLA58_01435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOR61487.1"
                     /translation="MTTDAAPIDPLMLDLFRVELENNTRMLEAGLVTAERDQTPERIE
                     PLMRAAHSIKGAARIVGLNTAVTLAHAMEDVLSAAQSGKRPLTSTAVDRLLKGNDFFA
                     SLSRLDIQELPAALERGTQEITVMAGELRNFLSSPQETTPAPTAAAQAEEKTAGDVPQ
                     KAQDIKADPSPAPRPAEPTPQKPLPAAVLENACPEPQTAPPAVRSRRSQDQGDGGLVR
                     VRSENMNRLMALAGECLVQAQSIKTVYQSLLVLKKEIFEIEKPLREFLERQEANEQDK
                     GAEDLQQTLSGAVSAQQIVSREIVRFERFSRRLEHLAHRLYNEVISSRMVPFSDGLHG
                     FPRLVRDLARETGKQVRFEIAGAATPVDRDILERLEAPLSHLIRNAVDHGMETPEERK
                     AAGKPAEGTLVLEARHVAGMLNISLTDDGRGINPEDLRGKVVEKGYVSQDMAGSLSHA
                     ELFEFLFLPGFSTARKVTEISGRGVGLDVVFNMAHEVGGTVRVDADAGRGARFHLQLP
                     LTLSVLRTLLLSIAGEAYALPLSRIDRVLSLAPADIREIEDRPYCTLDGEHVGILDAR
                     QLFGLPQASTPPAGFSVVIISDRLNRYGLVVDRVLGEENLVVRPLDSRLGKVPNVSAG
                     AILDDGSPVVIFDVDDLVRSIHQLLTTGKLQKIGRGRQALKTGKKRVLVVDDSLTVRE
                     VERRLLENAGYEVTTAIDGVDGWNKLTSHAFELLVSDVDMPRMNGIDLVRTVKTDPRL
                     KDMPVMIVSYKDREEDRLRGLEAGANYYLTKGSFHDESLLDAVRDLIGGPE"
     gene            37989..39026
                     /gene="cheB"
                     /locus_tag="PLA58_01440"
     CDS             37989..39026
                     /gene="cheB"
                     /locus_tag="PLA58_01440"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOR61488.1"
                     /translation="MTLKIAIAHHDENALKILRQIIAPDTGCAVIWSTGRGSDVIRQC
                     RHDLPDILLIDLALADVSGARLICDVMKETPCAILVLSNAREQQAGKVFEAMGCGALD
                     VISTPVLHSNGFLEGADVLIKKIDTMARLLGSASRTTKPAAAVRQTEKEPQPQLVAIG
                     SSTGGPKALATILAGLPREFPAAIVICQHVDLQFAGGLASWLNSQTPLSVAVAREGMR
                     VRPGVVLVAQTNDHLVLGPDGALHYTEHPRNYPYRPSVNALFESLRQYWPQNDVAVLL
                     TGMGRDGGEGLSALFKAGWHTIVQDEKTSIVYGMPAAAVELGGASEILPLDQIAGAIA
                     RKIINRKGTSL"
     gene            39023..40246
                     /locus_tag="PLA58_01445"
     CDS             39023..40246
                     /locus_tag="PLA58_01445"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HOR61489.1"
                     /translation="MTEKENLNPAPPGSHDPASTLTAHKIKVLLIDDQAIVGAAVKKM
                     LEPETDIVFSFCQDPTQAIPTAAAFQPTVILQDLIMPEIDGLTLVKFFRVHPQLKDVP
                     LIVLSSKEEAVTKADAFSLGANDYLVKLPDRIELIARIRYHSRGYINLLQRNEAYAAL
                     LASRQALAAELAQAADYVVSLMPPPLSDPTITTAWRFFPSAQLGGDSFGYHWLDENTF
                     AIYLLDVCGHGVGSALLSVSAFNAIRSQTLPDTDFHSPDRVLAALNNAFQMEHHNNLY
                     FTIWYGVFDRRTRELRFASGGHPPAFLISKEGTLTKLMTNNLFIGGLPESAYQDSAIT
                     VAPGANLYVFSDGVYEVDPPEGSMWTLDELGDYLLQHKMEAGEDIDALYRKMQDYHGQ
                     KILEDDFSMLAVHFT"
     gene            complement(40450..40728)
                     /locus_tag="PLA58_01450"
     CDS             complement(40450..40728)
                     /locus_tag="PLA58_01450"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOR61490.1"
                     /translation="MLLGLAIALQIGDGVTTFLALQKQGVREWNRILRRLFDRLGVVR
                     GLALAKSFGVAVCWVAYAYGGPFAVYTLGFIVVLYAVVVINNLKSLCR"
     gene            complement(41040..42227)
                     /locus_tag="PLA58_01455"
     CDS             complement(41040..42227)
                     /locus_tag="PLA58_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOR61491.1"
                     /translation="MKLLKIGLLLMLAVLVLLAGCAKKEAFRPEAPRPGEALVPADAR
                     QLDLVDDLDAESLLTAIDRSLLFYDGSGKNQIFRIADRQVSAQQMKETLIAFRKILVS
                     DIPSGEKNRRIAEEFLLLRAAGESGDGAVLFTGYYEPLLEGSLTRTEKYKYPLYRPPP
                     DIVVEKISKHETRISRRENGRLVPYYSRREIDVDGVLQGRGLELIWVSDPVELNSLHT
                     QGSGKIRLEDGRMLTVSYAQNNGRPFRSATQNLLNESKIDPGNASYRGFKAWLKGKSE
                     KEIYEILSYNERYIFFRFVESEPVGSLGQPVTPHRSIATDPAYFPQGALAFIRLRKPV
                     LDEDYRVVRRVEFSRFVLNQDKGSAIRGPGRVDLFCGFGPEAQAIAGSLKEKGELFFL
                     LLK"
     gene            complement(42224..42682)
                     /locus_tag="PLA58_01460"
     CDS             complement(42224..42682)
                     /locus_tag="PLA58_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOR61492.1"
                     /translation="MLRKARIGDVKTIHRLINVSAAKGEMLPRSLMDIYNSVRDFIVY
                     FDEEEQKIVGICAMNIIWENLAEIRSLYVDEAFRRRGIGRKLVEFCISDAITLELFRI
                     FTLTYKKEFFAGLGFKEVDRSILPEKVWSDCFRCPKFPDYCDETAMIIEL"
     gene            complement(42657..44378)
                     /gene="recN"
                     /locus_tag="PLA58_01465"
     CDS             complement(42657..44378)
                     /gene="recN"
                     /locus_tag="PLA58_01465"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOR61493.1"
                     /translation="MLRDLSIANFAIIDELRVSFDEGLNVISGETGAGKSILIGAVSL
                     LLGDRAAADMIRTQTDTATVEALFDIQGNRALQEKLAQMGFAAGDDLVIRRVISRSGK
                     NKAQINGQMATLANLAAISESLINICGQHEHQVILFAENHIDILDAFGGILDARREYA
                     AVYDRFQQCLGRIAKLEELRRRSAEKADWIRFQLEEIKSLNPRAGEDAQLADERKVLA
                     NARALADRADRAYALLYADSGSVGEQLKEALSQIREIQKIDPGLNLRPADVEGSYVIL
                     QEAALALRDYQKKLVFDPERLAAIDERQDALGRLKRKHGGTLEAVLARKREMEEELQS
                     VSGAEEELARLKKEKDAAAGELREKALGLSTMRQQAARSLERSVDREIHDLNMPHASF
                     AVKFLRRCPEDETACGPKGGDEIEFYLTANAGEEPKALHKIASGGELSRIVLALKNVL
                     TRIGSVDTVVFDEVDSGIGGATAEIVGRKLKTLSAHHQVICITHLPQIASFGGPHLRV
                     SKKIVGGRTSTVVEKIDDEQKIEEIGRMLGGVDVTETARDHARELLAAAGAAAGRSRN
                     AKKSAHR"
     gene            complement(44368..45261)
                     /locus_tag="PLA58_01470"
     CDS             complement(44368..45261)
                     /locus_tag="PLA58_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOR61494.1"
                     /translation="MKIKKVGIVANIEKENIAGFTGALKKWLEARGIRVALEANIAAA
                     VGGSGGFKLEDLAARADLIAVLGGDGTMLRTARNVAQHPVPIVGINLGTFGYLTEVNL
                     NETYAAFELILQGDFLTEKRMMLDVKIRRGNKTIASGIVLNDVVINRGNLSRIVELET
                     SVSGHYLTTYKSDGLIVSTPTGSTAYSLSAGGPIVYPGKELIIINPICPHTLTNRPII
                     FPENSDLQITLWSKESGATVTLDGQESYRISSGDVMTIRKSKFYTRLVLSPHRGYGEI
                     LRSKLGWGSLPSGAGKRKNAS"
     gene            45498..46586
                     /gene="rdgC"
                     /locus_tag="PLA58_01475"
     CDS             45498..46586
                     /gene="rdgC"
                     /locus_tag="PLA58_01475"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOR61495.1"
                     /translation="MGLIRGAFTFMQFSVDGKLPQAFTTFARSRIQGNAFREARSSTE
                     EKRMGWVSATDVLDADFENTPFSLGDYLIFSLRIDRKVIPPKLMKVRLMEEQRRFLSE
                     HQQTRIGKAMNEGLKEKVRLEIMTKSDPVPSFYDVLWAVGQNKVYFSSLSDKVADDFV
                     DYFKKTFSLGLKRIVPHEHPLALKNKAKGDAPTEDFTLIGREFLTWLWFKSEERNGAI
                     ALAKTDEVQLQLLKRIALEAGEGEYSQGVVCSGLHAELKEGKEAIRQGKKVKEAGILL
                     HRDRDEWEFNFKADAFYFQSLRMPAADPPETQEDAEGVLLERIYRIENAVRTIDRLYE
                     SFLTLRFSPEWTKNEKPRLAAWLRKEQE"
BASE COUNT        10325 a        13531 c        12537 g        10078 t
ORIGIN      
        1 gccatttttg tcatttcgac ttcttctttg tcatttcgac cgaagggaga aatccatgtc
       61 ccgaatgcaa tgagggatct taagatttct cgtcgctcac gctcctcgaa atgacatttt
      121 tcaaagctct cggctggtcg gcagtcatcc gacgaaacgg ggttcagtac ccgacatgag
      181 gcggccgaaa cagttcgcct tacgcctcgg cgacccgccg ggatattttt ttaagccggc
      241 tgatcttgtg gcgcaggagc gcggcttttt ccctgtcgct gtcttccagc gcccggccgt
      301 gcagaacttt caactcacgg attttcgcct tgacttgggg cctggtcagc ctgattttgc
      361 ctttgggttt gtgtttgtgc gcgcgccctt cgtccttgat gcccttggcc tgtttgataa
      421 aggcaaccag ctcttccttt ttcatgtcgt ggatggcttt ttcatgcggg aactcctcgg
      481 ccatttcccg cagctccttc acggtcaact tgtccaggtg ctgatcatca tggggtttgt
      541 gttctttctt gtgtgtttct tcggacatcg ctcatagacc tccttgtttg gtttcaggaa
      601 catcaacgtg caatgcgaaa aaggcaaacc agtttaactt tcctatacca caaccgcttt
      661 tgatgttcaa cccgagctgc cctattcctc cctattcttc ccattgacat cgcacggcgt
      721 ttgtcttatt ctttgagcaa ccccaatacc ggacagccgg gcgtaaatcg tttggctttg
      781 ttattcattc cttcaaggag aaaaatcgtc atgaacccga ccggtatttt gaaagaagaa
      841 catcaactcg tccttttgat gctgaagatt atggaagcgg tgtgcaaaaa gctggacttg
      901 ggccaggccg taaaaacgga agacctcgac gccatcatcg atttcatccg gggctttgcc
      961 gacaagtgcc accacgccaa ggaagaaaaa ctgcttttcc ccgcgctgga gaaagccggc
     1021 atgccgcaaa acgacggacc catcgccgtg atgctgcgag aacatgctat gggccgcggt
     1081 tttgccaaaa gcatggagga ggctatcggg cggatgaaaa acggcaacgc ctccgccgcc
     1141 ggaacgtttg tggaaaaagc ccgcggctac atccagcttt tacggaatca tatcgacaag
     1201 gaagaccatg ttctgtttat gatggcggac cagcgcctga accccgccgc gcaaaaagag
     1261 cttctggaag gcttcgaccg cgtggagcgt gaagagacgg gcgaaggggt tcacgaaaag
     1321 taccacgcct tgctgcaccg catgcgcgat acctatcttt aacggatttc gtccggcctt
     1381 tccggagcct gtcctcgccg acaaagagcg tcaacaggcc aaatggaaca aacggagcac
     1441 catggatttg tcattgaaat cggaaaacat agactgaatg ttcacaaggc ggtcaacgaa
     1501 tcttctctct caattttttt ccggccgctt ttttaactac aagaaccatc aaccagtgaa
     1561 cgagacatcc tcatgaaaga tcaagccaaa accaagcaga ccttgatcgg cgaactggct
     1621 tccttaagaa agcggattgc ccagttggag caattcgaat cggagcgaag gcaggtggag
     1681 aaggcgttgc gcgcaagtga aaaccgctac tacaaactct ttcaacacat gagcagcagc
     1741 gtcgcgattt atgaagccgt cgaagacggg gcggatttcg tgatccggga tttcaatgcc
     1801 gcggctgagc ggctggaaaa gattcagaag gaggaaatcc tggggcggcg ggttacggag
     1861 gtcttcccgg gcgtcagcga cttcgggctt ttttcggttt ttcaacgggt ctggaaaacg
     1921 ggcaattccg aagatcacaa cgtttcattt tatcaggaca accggatttc gggctggcgc
     1981 gaaaatcatg tttataaatt gccttccgga gaggtcgtgg cgatctatga cgatgttacc
     2041 gaccgcaagc aggcggaatg tgcgcttcgg gaaagcgagg agaaataccg cgccctcttt
     2101 gaaaatgccg gcgaggcgat ttttgtcgcc caggacggca gggtggcgtt tctcaatccc
     2161 atgacggccc agatcaccgg ttattccggc gaggagatca cttccaggcc atttgtcgat
     2221 tttatccatg cggatgaccg cgatatgatc actgaccgtc acacccgacg tcttcaaggc
     2281 gaagatattc cccggagtta cacttttcgg atgcttcgta aaaacggaag cttgctgtgg
     2341 gtgcagttgg atacggttct gatcaactgg caaggcaagc ccgcgacgct gaattttatg
     2401 accgacatca cggagcgcaa acggatcgag gatgcgcttc gggagagcga gcacgagcta
     2461 agggctcttt tcaaaagcat gctgaatgca tttgtgcttt tcgagtcggt ttttgaccgg
     2521 gacggaaatt ttgtcagcta ccggtttgtc tatatcaacg atgcatacga acgcatcacg
     2581 ggcgtcaaga acaacgaagt caggggaaaa accgtgcatg aggtctggcc caacacggag
     2641 gcaagctggg tcaaggcata cggtgacgtt gcaaccacag gaataccaag cactttcgat
     2701 atgtatcacg aaccgaccga aaaactttat cactgcaatg cctatcggcc ctgggacaca
     2761 aaaggtcgat tttgtgttgt gtttgaagac atcaccgagc gcaggcgggc ggaggaaatt
     2821 atccggatct ctgaagaaaa attcagccaa gccttccatt cgagtcctat tctgatggcc
     2881 atctcgacga tagaagaagg ccgttttatc gatgtgaacg aaaccgtttt gcgaaccctg
     2941 ttattctcca gagaagaagt catcggtaaa acttcttttg aattgggtat ttttgctgat
     3001 cccgtccagc gtcaggccat caggaaaaca acggaagaaa aaggttatgc gagaaacatc
     3061 gaagtacaga tggttgcgaa gaatggccaa atcattgaag gtctcttctc cgcagaaccg
     3121 atgaccatcc ataacgaaaa gtgctggctt accgttatgg tggatgtaac gaaacaaaag
     3181 caggcggaaa gggaaattca aagagcgaat gccttgctga actcgattgt cgagaatatc
     3241 ccaaacatga tcttcctcaa ggatgccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnctt gctgaactcg attgtcgaga atatcccaaa catgatcttc ctcaaggatg
     3421 ccggggaact ccggttcacc cgtttcaacc gggcgggaga agaactgctg ggtcattcca
     3481 gggacgacct gttgggaaag aacgattacg atttctttcc gaaggagcag gcggacttct
     3541 ttacacagaa ggaccgggaa gttttgcgcg ggaaggtaat tgtggacatc caggaagaac
     3601 cccttcagac ccgtgacagg ggagagcgaa tccttcacac caagaaagtg ccgattctaa
     3661 acgcgaacgg agagcccgaa tacctgatgg gcatctccga agacatcacc gaccggaagc
     3721 gggcggaggc ggagaagcga agactggaag agcgtttgcg cagggcggag aagatggagg
     3781 cgcttggtca actggcgggc ggcgtcgccc atgatctgaa caacgtcctg ggcgtccttt
     3841 ccggatattc cgagcttttg cttgccgaaa ttcccgaggg gcagaaagcc cggagacacg
     3901 tcgaaaagat cctccagtcc acggagaaag gcgcagccat cattcaggac ctgctgacct
     3961 tggcccggcg gggcgtcctg tcggaggaag tcattcaagt caacgatatc atttccggtt
     4021 tcaccagaac cccggctttt gagaacattc gggactatca tccgggcgtt tccttccgga
     4081 tggactgcga cccgaatctt ctcaacatca aaggctctcc cgtccatctg gagaagacgc
     4141 tgatgaacct ggtgtccaac gcggcggagt ccatcagcgg cgagggcgaa gtggttatcc
     4201 ggaccgagaa ccgctatctg gacaaagccg ttgtgggcta tgacgaggtc cgggaggggg
     4261 actatgtcgt tttgtccgta tccgatacgg ggacgggcat ttccgacgag caccgggaga
     4321 agatttttga gccgttttac acgaagaagg ccatgggccg aagcgggacg ggtctggggc
     4381 tcacgattgt ctggggaacg gtcaaggacc acaacgggta tattgatgtg tcaagtcgtg
     4441 tgggagaagg gacgacgttt accctgtatt ttcccgccac acgggatgaa cagaccgccc
     4501 cgccggaaag gacgccggtg gagcggtaca tgggcagggn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnng gggaatatta ataccgattt tgacatccac atccgctatt
     4681 gccgaaaccg gcgtcaaaag gatcagcgac aagaaaatca tccataaaaa ctttttcatt
     4741 gaatcttctc ctttcttaag cgaagttgcc cctccggtca ccgggaaaaa cggtgtgact
     4801 tgataatagg tggaaattta ccgctttgct gggacgattt caagccagta atcgtccgga
     4861 tcgctgatga agtaaatccc catcgccttg ttttcaaagc agatacagcc cattttttcg
     4921 tgcagcgcat gcgcggcggc aaaatcgtcc acccggaagg ccagatgaaa ttcattgtcc
     4981 cccaagttgt agggatccgt ccgctctctc atccaggtca gctccagttg atgcggcgtc
     5041 cggccgtcgc cgagatacac cagaacaaaa ctgccgtccg gcggctcata gcggcgcact
     5101 tccttcaatc caagcgcctc ccggtaaaag gccagacttt tttccagatt cagcacattg
     5161 aaattgttgt gggcaaacgt aaattccata tcctgctcct ctccacactg ttttcccgcg
     5221 ggtgaacacc cggaatccgc ctacaggcaa tccttcatca tcaattccac cagttccggc
     5281 gagatgtccc tgttttcgcc cagtttgacc ataccgtgcg cttttaaatt ctcaaccaca
     5341 tcgggaatga cgtccgcgcc gatatggtag gcgcaaagcc gcgtcggcac cttcatgctt
     5401 tcaaaaaaat cgcgtgtttt ttcgatggcg gcatcaatgc gggcggtatc ctccccggtt
     5461 gtaattcccc acacgcgttc ggcgtattgc agaagttttc ccttcttttc ccccgcgcgc
     5521 atcttcagca gcgccggcag caccaccgcc agggattttc cgtgatccag gccgcgcagc
     5581 gccgtcagtt catggccgat catatgcgtc gcccagtcct gcggaacacc gacgccgatc
     5641 agcccgttga ggccaagcgt ggcgcaccac atcaggttgg cgcgcacatc ataattctcc
     5701 ggttcggcca gggcccgggg tccttcctcg atcagggtga gcagcaggcc ctccgcgaaa
     5761 cgatcctgca gcttcccgtt gacgggaaac gtcaaatatt gctcggtcac atggacaaac
     5821 gtgtccacca cgccgttgcc gatctgctcc ggcggcagcg tataggtctt cgtgggatcg
     5881 agcacggaaa atttcggaaa cagcgacgga tgcatgaagg gaagcttggc gttcaaggcg
     5941 cgattcgtga tgaccgctcc gttgttcatc tcggatccgg tcgccggaat cgtcagaacc
     6001 gttccgaagg ggatcgcccc ctttgccttc ttgccgtaac tggcgacaat ttcccaggga
     6061 tctccctcat ggggaacggc tgcggcgata aactttgtcc cgtcgatcac ggaaccgccg
     6121 ccgacggcca gcagaaaatc gattttattt ttgcgcgccg tctccacaca ccgcatcagc
     6181 gtctcatagg tcggattggg ctcaatgcct ccaaattctt ccatcgcgca gctctgaagc
     6241 gccgccctga cctcatcata tacgccgttg cgcttgatgc tgccgcctcc gtaggtcatc
     6301 agaatctttg cccggggggg aatttctccg gcaatatcct tgatccttcc ctcgccaaaa
     6361 agaatttttg tcgggttata aaacgtaaaa ttgttcatat tccctccttt tgtgagatgg
     6421 attgcaaaag cctgcggaaa gattccaagg gctgggctcc cgtgatctgg tattggttgt
     6481 tgacaatgaa caggggaatt ccggtcaggc ccagcaaacg cccttccttt gccgcttgag
     6541 ccagccgggg cgcataacgt ccgtcttttg ccgccgccag cgcttcctgt tcgtcgagac
     6601 cgctttcggc cgcgacagcg cctaccacct ccgggcggcc aatgtcctgg cgccggctca
     6661 gatacgcccg aaacatgttt tcatgaaaga cgtcatgcct cccctggtcg cgggcatact
     6721 ccgaggccaa aagcgccaat cgggaattgg acagcacggt gtgttcattg aagacgatgc
     6781 ccagttcacg accccgcgac ttcaacccgg cgtaaaaggg cgcaaggtct ctgcctttaa
     6841 agcgttcgga aagcaacaga cctccgggcg gggtttcggg atgcagctcg tagctcaccc
     6901 actcttcctt aatcggatat tctttcttca accgatcgac aatcgccttg ccgatataac
     6961 agaagggtca gacatagtcg gaaaatattt taaaatgaaa ggtcatccgc ttcatcctcc
     7021 cgcgatccct cccaagggga gagaagaaag cattgaaaga aacgttgaca atctagctca
     7081 ggcgtttttc cagggcgttg agctcttttt tcagctctgc aggcagtcga tcgccgaatt
     7141 ttttataatg ttcgcggatg ccttcaattt cttttttcca ctcttcctta ttgacgctga
     7201 gcagttcctt catatcagcc gcgctgacgc tcaggccggt tgtgtcgatc gcatcctcgg
     7261 tgggcatgaa gccaatcgcg gtttcgctgg ctttgccttt gcccgccgac cgttcaaaga
     7321 cccatttcag cacgcggctg ttttcgccgt agccgggcca cagccatttg ccgtctgctg
     7381 tttttcggaa ccagttgaca tagaaaatct tcggcagttt gtccgctgat gtttttgttc
     7441 cgatgttgat ccagtgctgg aaataatccc ccatattgta tccgcagaaa ggcagcatgg
     7501 cgaaaggatc gcgcctcacc tggccgatgt tggcggaaat ggccgcggcg gtgatttcag
     7561 aaccgacaat cgaacccagg aatacgccgt gattccagtt gaacgactgg tgaaccagcg
     7621 gaacggtgga gggacggcgg ccgccgaaca aaaacgcgct gatgggcacg cccttgggat
     7681 tttcccagtc gggatcgatc gcggggcact ggcgagccgg cgccgtgaaa cgggagttgg
     7741 cgtgggcggc cactttaccg ctgtctttcg tccagacgtt gccggtccag tcgatgatcc
     7801 tgccggccgg ggcgtcgtat ccgatgcctt cccaccatac atcgttatct tccgtcaagg
     7861 cgacgttggt aaagatggtg ttcttttcaa tggccttcat ggcattgtag ttggagtcct
     7921 ctgaagtgcc gggagcgacg ccgaagaaac cggcttcggg attgatggcg taaagacgac
     7981 cgtcggcgcc gaatttcatc caggcgatgt cgtcgccgac cgtttccact ttccagcccg
     8041 ggatggtggg aatcaacatg gccagattgg tcttgccgca tgcggacggg aatgccgccg
     8101 ccacgtatct tttttcgcct tcgggattgg tgatgccgag aatcagcatg tgctcggcca
     8161 gccagccctg ctctcgggcc atcaccgacg caatgcgcaa cgcaaagcac tttttcccca
     8221 gaagggcgtt gccgccgtaa ccggagccat aggaccagat cgtcttttct tccgggaaat
     8281 gggaaatgta ctttctctcg atcggggcgc agggccactt gctgccgtcg gattcccctt
     8341 cggccagcgg tttgcccacc gagtgcaggc agggaatgaa cacgccgtct tcgcccagca
     8401 cgtccaggac cttctggccc acacgggtca tgatgtgcat attcacgacg acgtaaggcg
     8461 aatcggtaat ttcaatgcca atccgggcga tgggggatcc caccggcccc atgctgaacg
     8521 ggatcacgta catcgtgcgg cctttcatgc agccgtcgta aaggcctttc atcgttttct
     8581 tcaattcatc cggatcgatc cagttgttgg tggggcccgc ttcatccctg gacttcgacg
     8641 cgatataggt gcggtcttcc acacgcgcca cgtcggaagg atcggatcgg aacaggaagc
     8701 aatttggacg cttttccttg ttcaggggcg tggccaggcc cgaatcgacc ataattctga
     8761 tcatggaatc atattcattc ctggagccgt cgcaccagta aattttttcc ggtttacaca
     8821 ttttggccat gtcatctacc cactggacga gcttcttgtt tttggtcata ccgctgtcgt
     8881 tcattcaacg tctcctttgt ccttattttc aaagccctgc tttcataagc cggctttcgt
     8941 tttccaggca aaaccgcctt cttacggcaa gtaggctcca ctagttgaaa atagccaaaa
     9001 aagcaaatca tttttgaatg gcctgcaggg caacttgatt tcatcgcggc tcaagagacg
     9061 gcacaatgca acggccttcc cggacagtga cgggcggccg atatcccacg gccctatccg
     9121 atgctcctcg aatcggtttt catgaaaaac gtcaggcctc atctgctcaa acgcatgctc
     9181 cgcgcgccgc ttattttaaa tgcctggttt tccgatagcg gtaataccgc aggggaatga
     9241 cgatgacgaa aaagggaatc atgtatatgg cggcaaccag cggctcgacg ggaccgaaaa
     9301 actcggagga gaagacgatc acgagcacgt tgttgatgtt ccccagcatg accgctccgg
     9361 ccagatgatt atgcaggggc cccttccgga agaaaaagat tcccaccgcg caatagattg
     9421 ccgccaggac aaatacgacg acggtcgcca tgagtatcgc ggacggttct tccttgaaaa
     9481 acggcgccca gcggtaaaac actccgaggt tgatcagggc aaacatcagc agggcgacgg
     9541 gaaactgaat cttcagaatc ggcgcaacca gccggggcag cagatatcgg atgatttcca
     9601 cgatcagaat aggaataaaa atcaccatcg ccagcatttt aaacatggcc agaaaggaaa
     9661 tcgccgcatc tttcgcaatc acgatcttga tgagcgccgg cagcgtaaac ggcgcaagcg
     9721 cagaggtgat gacaaccacc accagaacca gcgatgagtt gcctttgacc atgttcgata
     9781 tcatcggcga aaccacgccg gtggaaacgc ccgtcaaaag cagggcggcg agcgcataat
     9841 ccggagcaac cgcgtaaaag acgccgtaaa gaaccaccgg caacaaggcg agcttgagag
     9901 cggtaaaacc aagaatctcc cggatgtgcc cccgaagcgc tttccacaca tccgccaaat
     9961 caatcgacaa acagctcaga aaaaaattga tcatcagaca gtaaacggga agcgcctgaa
    10021 aacgggcgcc gaaatcggga aaaagaatcg cgacggtcat ggacaggatc accacgacaa
    10081 ccagaatgaa atcatgaaga cgaaacggag tttgcatgat catgggatac cagatggcgc
    10141 ctatcttatc aacatctttc gcctttacaa cttgatgtcc gtcatggacg atggcacctt
    10201 gcgtgagaaa cgcctgtata aaacatagcc gtagatcaac aggtttacgg caatgaccag
    10261 tccgccgaga atatactggg tcaaggcgga aagccccgcc ggataaatca ccgggatcag
    10321 atattgaaca acgaagtctc ccgtgtaggg cgcttcaccg cccaaccggc gcaggtgatt
    10381 ctccagaggc gtcagcggac aaatccatcc ggcaaattcg atgaaggccc cccaacaggc
    10441 ggcgggaaga tgcagccaga ccatttgcgg ccagcgaagg atcagcaggc cgcccgtcac
    10501 aacgaaaaga ataaacaaaa aatgaatgat gatcagcgca tccgccagca ggctatacat
    10561 aggatttcct tccgcgggat aatataccgc cgcccggcat ttggnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnaggcta tacataggat ttccttccgc gggataatat
    10741 accgccgccc ggcatttgga tcaaccacca cgcagcaagc tgtcgatggg taaaatgaac
    10801 gtcatgatat cgcaacaggc tgcggagaat gaacgctcgt cccgcagcag cgggattcaa
    10861 ctgtaaaccg ccgtttttca tatccatgtt tttatgatag atatagctcc gttggataag
    10921 gagtcaagat gtttggctgg ttggcgaaaa aacgacgacg gaaaatcctg gaggcgcctt
    10981 ttcccttcga atgggaagcc atcatccgca agaatatggc gcattataaa ttgctggatg
    11041 aagacgaaca aaggcgtctt cgcgaacgga tcgccgtgtt catggccgaa aaaaactggg
    11101 agggatgcgg cggtcttgaa ctcacggacg aaattcgcgt caccatttcc gccatggcct
    11161 gcctgctgat attgaacctt ccgcgtcagg actacgaaaa tgttgaatcc atcctggtct
    11221 atccttctga gcggatcctt cccgaacaaa aaatcggttt ctttgaaacc gcgctcgcgc
    11281 cgctggaaac cccgggcccc atcagcggac aggctttcga acagggcccg atcctgctgg
    11341 tctgggacgc ggttcacgaa agcatccgtt atgccggata cggctacaat gtggtgtatc
    11401 atgaattcgc ccataaactg gacatgcagg atggcgtcgc ggacggcacc ccccgattga
    11461 gagaccgcgc ggaataccgc gactgggtca aaacctgttc acgcgagtac aaaaaactcc
    11521 tcaagaacgt ggaaagagga aagcggacat tcctggacga atacggcgcg aaagatgaag
    11581 ccgagttttt cgccgtcgcc acggaacatt tttttgacca gccgctcggc atgaagaggg
    11641 ccgtgccgga gctgtaccgc gttttgaaaa atttttaccg ccaggacccc gccgcccgcg
    11701 aaagccagta cgcggcggcg cgggaagaac aaacccgaaa aaccgaagcg gcctttgcaa
    11761 aagcgacgga cgaaaagaag ggagacaggg aatgatcgac tatatcgaac ggcatgaaac
    11821 ctacgtcagg gaattgctga caggccatcc ggaaaaagaa acgcttgccg aactgctggt
    11881 ttatcacgac cgtcagattt cctgggtgca gcaggaacgt ctggcccatc tgatcacgat
    11941 gatgtttgta tgtcttttct tcctcctggc cttcggatgg acgcttatcc atttcacgct
    12001 gccgtatatc ctgctgaccg ccctgcttct gatcctttcg gccgcctaca tcctgcacta
    12061 ctaccgcctg gaaaacagcg ttcagcgctg gtatgttctg tccaatcaaa tcagagaaaa
    12121 actgcttcag gcaaaataaa tgcaataaaa aaagcccctt gtccatttgg acaaggggcc
    12181 ttaatctttt aatctttagc cttcagtctt caaccttcag cctttttctt acattcccat
    12241 tccgggataa ccgcctccgg gaggcatacc gggcattccg ccgcctgcgc ccttctcttc
    12301 gggcttgtcg gcgatcatgc actgggtggt cagcagcaga gcggcaaccg aagccgcgtt
    12361 ctgcagggca aagcgcgtca ccttggtcgg atcgatgacg ccggcggcga tcatgtcttc
    12421 gtactcatcc gtccgggcgt tgaatccgta ggaacctttc ttctctttca ctttttcgac
    12481 cacaatgctg ccttccaggc ccgcgttgca ggcgatcatt ttgatcggtt cttcgatggc
    12541 tttcttgacg atgttcacgc cgatctgttc gtcgccgtcc agggccagtt tgaccagagc
    12601 gggcagggtg cgcagatacg caacgccgcc gcctggaaca ataccttcct ccacggccgc
    12661 acgggtggca tggagcgcgt cttccacgcg ggctttcttt tccttcattt cggtttcggt
    12721 cgcggcgccg actttaatca cggccacgcc gccgaccagt ttggccagcc gttcctgaag
    12781 tttctcgcga tcgtaatcgg aggtggtctc ttcgatctgg gcgcggatct gtttgacgcg
    12841 gccttccagt tcggcgcgct ttcctgcgcc gtcgatgatc gtggtgttgt ccttgtcgat
    12901 ggtgatcttc ttggcgcgtc cgaggtcttc gattttcacg ttctccagtt tgtagcccat
    12961 gtcttcggaa atcatcttgc cgccggtgag gatggcgatg tcttccagca tggccttgcg
    13021 gcgatcgccg aagccggggg ctttaacggc cgccacatgc agcgtgccgc ggatcttgtt
    13081 gaccaccagg gtggccagcg cttcgccttc gatgtcttcg gcgatgatga ggagcgggcg
    13141 gcccattttg gcgatctgtt cgaggatcgg cagcagatct ttcattccgc tgattttctt
    13201 ttcatagatc aggatgagcg cgtcttccag cgcgacaatc atcttgtcgg gattcgtgac
    13261 gaaatagggg gacaggtagc cgcggtcgaa ctgcatgcct tccacgatct ccagttccgt
    13321 ttcgagccct ttggcctctt ccacagtgat gacgccttcc ttgccgacct tgtccatcgc
    13381 gccggcgatg atgttgccga tggtttcatc gttgttggcg gagatagtgc cgacctgggc
    13441 gatttctttc tggtccttgg tgggtttgct cattttgccg agctctttga cgacggtttc
    13501 cacggccttg tcgatgccgc gtttcacgtc catgggattg gagcctgcgg cgacggcttt
    13561 aacgccttcg cggtaaatgg cctgggccag gatggtggcg gtggtggtgc cgtcgcctgc
    13621 gacgtcgctg gtcttgctgg ccacttcctt gaccatctgc gcgcccatgt tttcaaattt
    13681 gtcttcgagt tcgatttcct tggcgacggt gacgccgtcc tttgtcacgg tgggagagcc
    13741 aaagctcttg tcaataatca cattgcggcc tttggggccg agggttacct tcacggcatc
    13801 ggccagggtg ttgacgccct tcaagaccga ttttcttgct tcttcatcgt aaagaagtat
    13861 ttttgctccc attgtacaga ttcctcctta tattttctta tttttcgata atgcccagaa
    13921 tgtcgtcttc tctcatgatg aggtattctt cgccgtcaat tttcacttcg gtgccgccgt
    13981 atttgctgaa aaggactttg tcgcccacgg tgacttctgg tttgatcagt ttgccgtcat
    14041 ccgccacttt gcctttgccg accgcaatca ctttgccttc aatcggtttt tccttggccg
    14101 tatcggggat gatgatcccg cccttggttt tggtttcctc ctccaatcgt tttacaatga
    14161 tccgatcctg taagggtctg attttcatac tgattcttct cctttcttcc ttataaatat
    14221 tgtaaagtaa accatcgaat tagcggcgtt attttatgca cgatttcccc cctgtcaagg
    14281 catggagaaa ctatttttcg ggaattctct gcaatgtttt cagaagagga tttccagcag
    14341 ttccttcagg gaagtgatgg tccggatttc catttttgcc gtttgggtca gggaggcgat
    14401 attggttttg ggaacaatgc accggttaaa tcccagacgg gccgcctcct tcacccgtat
    14461 atcaatctga gagatcgccc gcacctcgcc cgtgagcccg acttcgccca gaaccaccgt
    14521 ccgggcgtcc accggccggt ccagaaaact ggacgccatg gccgagacaa cggccagatc
    14581 cacggccggc tcttccacac gtacgccgcc cgcaacgttc agaaaaatgt ccgacccgcc
    14641 caggttgagg ccgcagatct tgtccagcac cgccaccaaa agcgaaaccc ggttgtggtc
    14701 cacaccgatg gccgttcgcc tgggcatgcc taaattggag gcgctcacca gcgcctggat
    14761 ttccaccaga atcggacggg tgccttcgag gcttgccagc accaccgatc cgggagcgtt
    14821 ttccggccgt tccgccagaa aaaaggcgga aggattgggc acttccgtaa gcccgcggtc
    14881 gcgcatttca aaaacgccga tttcgttggc gggtccgaag cgatttttca tgctgcggac
    14941 aatccggtag gcgtgcccgg agtcgccctc aaaatacaat accgtatcca ccatgtgctc
    15001 aagcactttt ggcccggcta tggcgccgtc cttcgttaca tggccgacca ggaacaatgg
    15061 aacggcgctt ttcttggcgg caagaatgag ccgcgacgac gcttcgcgga cctgccccac
    15121 gcttcccggc gcggacatca gatcgccgga ataaaccgtt tgaacggaat cgatcaccac
    15181 aacggccggt tcgattttcc ggatctgctc cagaatcttc tccagcgaca cttccaccag
    15241 caccagcaaa tcgcgggcct gcgcgccgat ccggtcggcg cggagcctga tctgggaggc
    15301 ggactcctcg ccggaaacat agagcacttt ccggccgccc gcagccagat ttttcatcat
    15361 ctgcagcatc agcgtcgatt tgccgatacc cggttcgccg ccgatgagaa tggccgagcc
    15421 actgacgatg cctcccccca gcacgcgatc aatttcagcc atgccggtga cgattcgctt
    15481 accttcctcg gccggaatgt cgaatatggg aaggggcttg cccccgaaca ccagatcggg
    15541 acggccgccc gcctccggat ccgtcaccag ttcttcggca aagcagttcc agccgttgca
    15601 ggacggacac ttgcccagcc acttgggcga catatagccg cagtgctgac aaaagaagct
    15661 ggtcttgctc tttttcaaaa ggcatttctc cttgacttgg gtgagtcaca agagcatatt
    15721 ttccgtaaaa acgccaatac gaatttaaaa ggatcaatgg atgatacgca taagaatact
    15781 catcttatca gccattccgg tgtttgtctt ttctcttccc gttttcggtt tttttgacga
    15841 tatcaggctg agcagttatg cccaaaaaca gcagattcat gaaatcattg ccatgctgca
    15901 gccgaagctg gacaaaggcg ataccttgaa tccccttcag atttatttgc tggctgcctc
    15961 ctacacttcc acccgtgact atcgcaaggc gcttatgacc atcgacatca tgcaaaaaca
    16021 aatcgaccgg ggggaaagaa cttatttagg cagtgatttt accgtctatc cgcaaatcct
    16081 gcgcggaggc atttatctgg gccagggaga ttttctgaaa tctattgcgg aaggcgatct
    16141 tgcgtatcgc ttgctccatg aaaacggccg tgaaaagcag aatttttaca catctcaact
    16201 gatcgaaata tacgattttc tgggcgttgc ccacgctctg gccggccatc agacggaagc
    16261 tcaaaaaata gccggcgctc tgcaaaaggt cagcattacg gtgatgaacg gtcctccaaa
    16321 atacagcgct ctggcgcgga tctacatggc tttgaaagaa tacgacaagg cgctgacggc
    16381 catcaggaat ccaagtgcaa gggttacagg atttattacc gccttctacg atcagacttt
    16441 tcaggaaatt cccaaattgt atattttatc caaaagcctg tatgaaacga gcaacctgcc
    16501 ggaagccagg cagggctatg atcagctttt acgacacccg cagatcgggc agatcggcgg
    16561 catatactgg attgtgcttt gcgaccgggc aaaaatcgcg ttgtcggaag gacagaaaag
    16621 catggccgaa gacctgctga aaaaagcggt tcaggtgatc gagcagcagc gttcatcgat
    16681 caatacggag gcggggcgca tcggttttgt cggcgacaaa caggatgcct atgcgcagtt
    16741 aattgatctg cttctgtccg ataaacggta cccggaagct tttgcatacg tggaacgctc
    16801 caaagcgcgc gccctggttg atttgctttc ctcgcagaag aatattccgg tgcgggggga
    16861 aaacgttcag cggttgaaaa tgaatttgaa tcagatggcg gaggcggaaa gcgaactggc
    16921 cgccgtatct tccgcggcaa accgggagtc tcaaaataaa acccgatcgg ttttgctgac
    16981 cctgaaaaag gagctgggag aaaaagctcc cgaaacagcc gctctggtta cggttcaggc
    17041 gccttccaca acagacattc aaaaattaat cgggtcggat gaaaccctgc ttgaatatta
    17101 ttcaacagga cggaactggt atgtttttgt gttgtcgccg tcatccgttg ccgtttacaa
    17161 attaacgaca gatgatcttg cgaaagatgt ccatgatttc cgtaaaatga ttacgctgaa
    17221 cgcttccgaa agttacaaag cccgggcgga agcgctttac cgtcagcttt tcgcgttcgt
    17281 agagccgtcc atcaagacca ggaagctgat cattgttcca cacggcgctt tgcattacct
    17341 gcctttttgc gctttagctc agggcgggaa ttatctgatt gaccgctaca gcatccggac
    17401 gctgcccagc gccagtgttt tgagttttct gaaagcgcgg accggtcagc cgcgcaccgg
    17461 ccgttcgctg attctgggaa atcccaaatt ggatgatccc aaatacgatc tgcaattcgc
    17521 ccaggcggaa gcgctggctg tcggggccat cctgccaaac tcaaaggtac tgctgcgctc
    17581 agaggcgacc aaaacgaatt tcatggcgga aagcagccaa ttttccatca ttcatctggc
    17641 cgtgcatgga acctttgatc cggataagcc gcttgattca tcattgctgc tctccgggga
    17701 caaagtcagc gatggcttgc tgaccagcgc cgatctttat cgcctttcgc tccatgccga
    17761 tctggttgtg ctcagcgcgt gcgaaaccgc cctggggaaa gtggccagcg gcgatgatgt
    17821 cataggcttc acgcggggat ttttatacgc gggagcgggc tcgctgatct ccagtttgtg
    17881 gcaggtcgat gatcaggcca cgcatgatct gatggtcgat ttttattcca accggaagac
    17941 gatgcccaag gatgaatctc tgagacaggc tcaaataaag gttaaacaaa aatatcctca
    18001 ccctttttac tgggcggcct tcctgctcac gggcaatgcg ctttagaatg ccgtttgttt
    18061 tatcgccttt ttcgcgagcc ggattgtttt ttaccagcct tgtgtagggc ggcgtaccga
    18121 cgaccggcaa gtcgatgaac ttctgaaaag cgggttgaaa tttacggaaa taatcgccgg
    18181 ccagttttgt gtcggatgtg ggcagttcca gcgtaagcgt gaaaatattg cgttcatgtc
    18241 cggcatagct ccccggagac cccggatatt aaccgaactt tttcagggga tgatgggctt
    18301 ctctggatat tttttctgcg tttgaagatc gtcctgctca acgaatatcg atgaacggtc
    18361 agtggtggaa tcgtcgtttt catgctttta tgatttcctg ttcggcgcgt ttcagccttt
    18421 ttaccgcaga ctcctttccc aaaatgacaa atactttatc cagctccggg cccttgattt
    18481 taccggtcag cgccgcccgg accggcatga acaaatcttt tcccctggcg cctgattttt
    18541 cttttgcgtg ccggatcgca gcggcgtaaa tttccgcggc gtcgccttgc gcccgattca
    18601 gatacgcgcc aaaagccccg accacctcgc gggcgccggg cgccgccagc agccttcggg
    18661 cttcctgcga cggggaatag cgttcatcaa aaaataaatc gatgtgatcc ccgatctccg
    18721 ccagcgtcgt cagttcttct ttcacgatgc cgatgatctc cttaagccac agagggtcca
    18781 gtgtttccgg ctgataaccc gcctcgcgta aaaacggctg aagtctttcc agcaaatcgc
    18841 cggcggtcag gtttcttatg taaagcgcgt tgagccagcg gagcttgtct tcatcgaaga
    18901 gagcgccgct tttggacgcc cggaaaagcg aaaacgcccc gatcatttcg tcgcgggaaa
    18961 gaatttccct gcctccggca aaggagcttc ccagaagccc cagataattg agcagcgctt
    19021 cgggcaggaa cccctgtttg cggaattcgc ccacggacac ggagccgtgg cgcttgccga
    19081 gcttggcgtg gtcttttccc agaatcagac aatggtgggc aaacaccggc tcgtcaaatc
    19141 caaaagcccg atacagcaga atttgcaagg cggtgttgga cagatgatct tcgccgcgga
    19201 tgacatgcga aatcgccatc agatggtcat cgatcacgac cgcaaagtta taggcgggca
    19261 ttccgttgga ccgcacgata atgaaatcgc ccatggcttc gccttcaaac cgcatcggcc
    19321 cccggatcag gtcatgaaat tcaatcgtcg tccggggaac cttgaaacga taggaaggct
    19381 tccggccgga cgcggccagc gcttcgcgtt ctttttcgct gagatttcgg catttccccg
    19441 aatagcgcgg catgcgcctg gcgagaatca gcgtccggcg ctcctcttcc agctcttctt
    19501 ccgtgcaata gcagggatac gccaggtccg cgtccatcag ttttttcaga tgcgccgcat
    19561 agagatccag cctttcactc tgccggtagg ggccataggc gccgccgatg tccggtcctt
    19621 cgtcccagtc aagccccagc catttcaagt cctccagaag accggtctgg taggccgaat
    19681 cgctgcggga ttcatccgtg tcttcaatcc gcagaatgaa acaaccgccc tcgcgccggg
    19741 cgaacatcca gttgaacaat gcggtgcgcg cattgcccac atgcagatct ccggtgggag
    19801 acggcgcaaa acggactctg gccttatgct ccatccgcga tcccttccgt gccgcaggcc
    19861 gtcgcccaaa cggcgattcc ctcgccgcgg cccgcaaagc ccatctcctc gttggtcttg
    19921 gctttgatgt tcacggccgt ttccggaatc ttgagcgccc gggcaatgtt ggctgccatg
    19981 gcctgcgcgt agggcgccag cctgggtttc tccagcacga ccgtggcgtc gatgttctcg
    20041 atgctcacgc ctctggattc gacgatcctt ccgacgcgct ccagaaggac caggctggaa
    20101 atgtctttaa acgcgggatc gttatccgga aaatgccggc cgatgtcccc cgctccggcc
    20161 atgcccaaaa gcgcgtcaca gacggcgtgc aggagcgcgt cggcatcgga atgccccgcg
    20221 agcccgcggt caaaggggat ttccacgccg cccagcttaa gtttcctgtc ctttgcgaac
    20281 cgatggctgt cgtatcccaa tccggcgcgg ggacggcgct gcctggccgc cgcggctttc
    20341 agaagagact ccgccatttt cacatcttcc ggcgttgtga ttttcatgtt ttcatcggat
    20401 cccgtaatca tttggacctt tgcgccgatt ctttccgcca gcgacgcgtc atccgttccg
    20461 taataatggt ctttttcggc cgcctcatac gcgcggcgca aaagattgta tgaaaacgcc
    20521 tggggcgtct gtgtctgcca taaattgcgg cggggaagcg ttgttttgac gatgccgtcc
    20581 tccgtcgttt ccttaatggt atcctttgcg ggaattccca gggaggcggc gccgcacccg
    20641 gcggccgcgg ccagaacccg aataatcatg tcttcggtga caaaaggccg gacggcatca
    20701 tgaaccatga cgatgtcgca gggcaactta acggcccgca ggccgcaggc gacggaatcc
    20761 tggcgctgtc tgcctccggc aacaacggca gccactttgg tgagattgta tttctccacc
    20821 acctgtttcc ggacggacaa aacatcatca cccgggacga ccacaatgat gtcggaaatc
    20881 gcctccactt tctgaaacac aagaagcgtg cgcaccagca ccggcaaagc atggagcaaa
    20941 agatactgct tggccatcgg agcgcccacc cttttgcctg agccgccggc gggaataata
    21001 gccactgttt tcatagcaaa tctcaaaaaa aacaggcccg aaaagccatt ttccgggcct
    21061 gttccggatg tttttcctcc cggccaaaaa gatgcaggca acaggtcgat agctttgaaa
    21121 aatgtcattt cgaggagcgt aagcgacgag aaaccttaag gattgccgtt tgttcaagat
    21181 ttctcccttc ggtcgaaatg acaaaaatgg ccgttattca aagctctcag gtctatgcat
    21241 tgccgctttt cttgtcggcg gcttccttga gctccgtgaa gatcattcgg ccggccgtcg
    21301 tctgcagcac gctggtgaca accacatcga cggtcgtgtt gatcattttt tgcgcgctgt
    21361 ccacgacgat catggttccg tcgtccagat aaccgacgcc ctgcccctgt tccttgcctt
    21421 cccggatgat cttcaccgtc atctgctctc cgggcaaaac caccggtttc atggcattgg
    21481 ccagttcgtt gacgttgagg acgcgcacac cctgcaactc tgcaactttg ttcaggttat
    21541 aatcgttggt caggagtttg gcgtttagtt tctgggccag ggccaccagt ttcccgtcca
    21601 cgccttttat tttgggaaaa tcctgctcga caatttcaat attgatggcc gtgctcttct
    21661 gcatgcggtt taaaacatcc agcccgcggc ggccccgtga gcgcttcatg gaatccgagg
    21721 aatcggcaat gaactggagc tcattgagga caaaacgcgg aacgatcagt tctccgtcca
    21781 caaaacccgt atcgcagatg tccgcgattc ttccgtcaat gatcacgctg gtatccagca
    21841 ggcggtgatc catcagtttc ctggaatcgg gggtgcgaaa attgacttcc tccgcctttt
    21901 tcgagccgat cacaagcccc aggtacccca gaatgccgat caggagcaga tagatccagg
    21961 gaacaacctg ctgattctgc gggatccgga tgaccatatt caaaccaaaa ccgatcatca
    22021 gcgcgatcaa cagaccgatg atcatgccga ccacaccacc cgcgatgatt ctcacggatt
    22081 gcttgcggat gttgcgttcg acccgaatga ccagcagcgc aacgaccaag cctataatta
    22141 agccgaaaat tgcctgaaaa acagcgtatg gactggctgc cgccatggcg tagtaagcga
    22201 taaaataacc gctgaccgaa caaatcaaaa ccaataatgc tcttacgatc aatcgtttca
    22261 cctcccttcg aataaaagaa tgaatggaat cttataaatc aggaataaaa ttaagcgaat
    22321 gttgcttctt gtcaaatgcg ttgaatatgg aggtcccgca cacgatcagg gaaagagggt
    22381 ttgttcttct ttaagttttc aaagagcgaa aaacagacag acccgatatc cattcgctat
    22441 tgcacggtga aaatcatctg caaatcgcgt tcaaccttgg cttcctgcgc gttggtggcg
    22501 acggagattt ccttcacaag cagatttttc gcggtatcca tcatcttccg ctcgccgaaa
    22561 gaaaggtttt tatcgctttt caaaatgaac aaatccctta aaacactggc aatttcaaaa
    22621 acagaccctg ttttgatctt ctccagatac tcccggtagc gcttgttcca ggtttgtttg
    22681 tctatggtga catctttgtt tcgcaggatt tcatacacct tggggatttc cttctccaga
    22741 atgacttccc tgagccccac agccttggca gaattggttg gaatcatgat cttcatgccg
    22801 ttgcccataa ttttcatgac ataaaaaggc tgctttttcc ccatcacttc cctattctca
    22861 atagcctcta tgacaccgac cccctgggcc ggatacactg ccaaatcacc tactttaaac
    22921 attggatacc tgcctttcta ttagagatca ggactttatc actttttctt gatttagtca
    22981 atgataaata cagtttatga aaagcttcgg aaaggttgcc cggggttgaa aaaatggctt
    23041 cttacggcaa ataaaacaat ggattccggg ctttgccctt gatgcggatc tcaaaattaa
    23101 aataggtttc ccccgcctca tcggttttcc cggcatgacc aatcgtctgg cctttgccca
    23161 cgtcctggtc cgtccggacg gatcttttgc tgagatgggt gtaaaccgtt gcgaagtcgt
    23221 tggcatgccg gataatcacg gtttcgccga aatcttttaa atttgccgaa aaaataaccg
    23281 tcccggaagc cgccgctttc accctggttc ccgccggaca ggcgatttta atccagttgt
    23341 gatacgtttt gttcggttga atcccgaagc gggttttcac ttctccgcga aacggccacg
    23401 cgaaacggcc tttctccatc accacttttt cttcagaaac ctggggcttt tccctgccgg
    23461 gtatgtccgc tttaggcgct ggtaaaaccg tcccggtgtc cggcctggat ggtttggccg
    23521 tcccggccgg cgtctgcgac gttttctcct gcgacggcaa gggtttggca agtgttgaag
    23581 gcttttcccc accggaaggc ttgatatcct tgaccttttc ttccttgccg gcatccgcgg
    23641 ccgctttccc cgcagacacc atgacgtctt caatcacctg atcggcattc ggaatgaaaa
    23701 tgacggatcc ttcccgaatg ctggctacgt cctcaatatt attgatttca gcaagttcct
    23761 gcaggccggt tgaataagcg cgggcgatgc tgtagagggt ttcccctttt ttgacgatat
    23821 ggtaaacgcc gctggcctgg gttttctgca tctgggaatt cgcgcagcca accggcaaaa
    23881 gaagcgccgc gatgcacaat aaaaagcacg ctccgaaata ccggcctcga ataacggcaa
    23941 gaaaccggcg ggcgaaggca gaccggatca ttctttccat ccgctttccc cgatcaggct
    24001 gacaaagcgg cagcccccca gatcttcctt tttcagttcg gacggatctt cagtcgagcg
    24061 ggtaagttta taaagcgtct gcacttcccg gccgcccacc gggacaacca ttctgccccc
    24121 gggagaaagc tgcgcgacca gatgctgtgg aaattgcgga gcggcggcgg ttgtaatgat
    24181 cgcatcgaaa ggcgcctcct ctttccatcc gtacgatccg tcgccgacgc gaatggcgac
    24241 gttatagcaa ttcagccggt ccagaatttt gcgggcgcgc gaggccagca ccgccacccg
    24301 ttcgatggaa aagacccggt cggccagttt ggccaggata gccgtctggt atcccgatcc
    24361 cgtgccgatt tccagaaccc gctccatccc tttcaattcc agggcctgtg tcatcagcgc
    24421 gacgatatag ggctgggaaa tcgtctgccg ctctccgatg ggcagcggat tgtcgctgta
    24481 agcctggtta atcaacccct catccacgaa aaggtgacgc gggaccgtct ccatcacttt
    24541 caaaacacgg tcgtcacgga tggaacgacc gcgaatctgc gtttccacca tgcgaatgcg
    24601 ctgctttttg tacctgtcca tcaaaccgcc tgcatctttt tctttttgga gggaatccgg
    24661 tagagtctct gaaccgtttc gtgtctgcgc aatcccaggg cttcccgggt tacaatcaaa
    24721 taataatatt gaatctcggc tttttggcag gccgcgttaa attgctcaac aatttcgttg
    24781 atttcttcaa aaaccgactg cttttccttc ggatgcgcgc cctccggcga gccggtttgc
    24841 agcgattgcc agcgaaacat catttccccg aaccgtcggt ctaaccggtc cagcttcgca
    24901 agcgcatcct cgacggcaaa acccgccctg gccagaaccg ccgctctttc ccgcagcagt
    24961 tcttcacaca aactttcctg gtgtttctct cttccgctat gcagtgcctg cgccataaaa
    25021 ccttcttacc gattctcgag ggcctctatc attcaagcgc ttaattttca agttaaaagc
    25081 gcttacttcc gaaacgaaga aatttttttc tttacagttc aatattattt cgttacatag
    25141 acgccccgat aaacgcaacc gacaaactgg tgctccccat gcgctctgaa acgacatttc
    25201 ccatgtcccg ggccgtcctc aatgcctatg ccgaaggaca ggaagacgaa acgctgctgc
    25261 ctctggttgg ggtcaaccaa cagcagaccc ccctgggaag aatccagccg ggcgactcga
    25321 tcatctttta caatatccgc ggcgaacgcg aaattgaatt gagccgcagt ttcacggaag
    25381 aaaacttccg gaaattcccc gtccaccccg ggcttcccgt gcacttcacc accatgatcg
    25441 agtattcgaa agacctgaac accggggtcg cttttccgcc ccggggcgtt ctttccgata
    25501 ctttaagcga catcatcgcg gcgcatggac tggcgcaggc aaagataacg gaggcggaga
    25561 aagccgtcca tatcagctat tttttcaacg ggaagaaaag tgatccgctg ccgaaagaag
    25621 aacgaatcgt tgtcccgacg cgcaaggacg tcaggctgtt cgatgaagcg cccgaaatgt
    25681 ccattgatga aatttcacgg gaggccgaag ccagaattca ggatccctcc cgccgtttca
    25741 tcgtcgtcaa tttcccgaat gtggacgtcg tcggccacat tgaaaacgaa ccggcgatcc
    25801 tccgggcggt ggaggcggtg gaccggcaaa ccggagcgct ggtggaccag gcgcttcgtc
    25861 agaacatggc cgtcatcgtc accgccgatc acggcaccgt ggaaaaatgg ctgtatccgg
    25921 acggaggcgt ggataccgga catacggaca gccccgtgcc cttcattcta ttgcatccgg
    25981 agcaaaaatt ctctctgaaa tccgaagggg aactgaccga catcgcaccg acgattctgg
    26041 atttgatggg cctgcccgtc cccgcttcca tgaccggctc gtccctgatt cagaaaaagc
    26101 cttctccggt cgtccccgtt tcgcgtctgc tcctgctcat tctggacggc tggggccttt
    26161 gtgaaaacac aaagggcaac ctgatcgcct gcgcccaaac gcctgtgatg gaccggctgc
    26221 tccgcgattg ccctcacacg cggctggccg cctccggacc ggctgtgggg cttcccgccg
    26281 ggaccgtggg caattccgaa gcggggcatc tgcacatcgg tgcaggccgg cggatttatt
    26341 ccgaccgcct gctgatcgat caggcgattg cccggaagga atttcaccga aacccggctt
    26401 ttttgcgcgc catggaaaac gcccgacaaa acaaccgcgc gctgcacctc atgggcatcg
    26461 tgtctttttt cagctcacac ggatccatcg aacatctgtt ggccctgatg gaaatggcca
    26521 aacagcaggg cgtttccgaa ttgtatatac acgccatgct ggggcggcgc ggcgaacagg
    26581 ccgaaagcgg cgcccgctac atccggcagg tggaagaaaa atgccggtcg ctgggattgg
    26641 gacaggtggt gtccgtgatc ggacgatact ggtcgatgga tcgcgaagaa aactgggacc
    26701 ggatcgaaaa aacctaccgg atgctggttt acggagaagg aaccccggtc cccacggagg
    26761 gatgatcgtg aaaaccactg tgaatcaaac cgtcatcgaa ctcgtttgcg gagacattac
    26821 acaggaggaa accgacgcca tcgtcaacgc cgccaacagc cgcctggcag gcggcgcagg
    26881 cgtggacggg gcgattcacc gcgccggcgg ccccgccatt atggccgaat gccgcaagat
    26941 cggaggctgc ccgaccggcg aggccgtcat cacaacaggc ggcaacctca aagcccgttt
    27001 tgtcatccat accgtcggcc ccgtgtaccg cggcggagga aaaggcgaag cgggcctgct
    27061 ggcctcggcg tatcaaaaca gcctgaacct cgcccgcaaa aaaggcctcg ccagcctatc
    27121 ctttccggcc atcagctgcg gggtttacgg ataccccctc cgggaagccg cttccctggc
    27181 gctttcaacc tgcgtcgctt tcgcgaaaaa ccatccggag attcggcaca tccgccatgt
    27241 tttgttcgat caacggacct acgacatttt cgcgcaggaa ttgaaaaaaa tcacgccgtg
    27301 aaaggtgcgc cttcatgaac gccaagccga cttacgagga actggagcaa aaagtcgccc
    27361 tgttgtcccg gaaagtgaac gagcagcagg ccatcgaaaa tcaataccgc aacctggtgg
    27421 attccaccag cgactccctg tatctggtgg acgccgacgg ccgctatctg ttcatcaatc
    27481 acaaccacgc gcggcgtctg aaaatacagc aaaacgacct tgccggcctt tcctacaagg
    27541 acattcactc gcccgagcaa aacgccaaat tcgaacgaaa gatccaaggc gtctatcaaa
    27601 cgggcctgtc cgattccgac gagcaccaca gtttgcggga cggccggtat tttctgcgca
    27661 ccttcagccc ggtgcgggat tccacccacc ctgaacgcgt cattgccgtg gcggtggtct
    27721 ccaaggacat tacccccaga agggaagccg aagaagccct gagaagatcc gaggagaaat
    27781 accgccggtt gattgaatat tccagcgagg cgatttttat catcagcggc gggaaaatca
    27841 gttttgccaa caaccgcacg gaaaagatgc tggggtatcc gctggccgat cttcaggcca
    27901 tgcctttcct ggagctggtg attgccgagg atcgcgatat gatcgacgag cggcagcaag
    27961 ccgttcttca ggaaagcgaa ccgtcgttga acaaccgttt ccggatggtc cgccgggacg
    28021 aatcgaccat ctgggtggcc gccaacagtg tcctgatgcg ctgggaaaac gctccggcga
    28081 cgctgaactt catgcgcgac atcacccggc aaaagaaggc ggaggccaag ctgctgcagg
    28141 cgcagaaaat ggaatccatc ggcacgctgg ccggaggcat cgctcatgat ttcaacaacc
    28201 tgctcatggg cattcagggc catgcctctc tggcgctttt gaaccttcac cccggtgatc
    28261 ccaattacga acatatccgg accatcgaaa ccctggtcgt cagcggcgcc ggtctgacca
    28321 ggcagctgct cggctttgcc cgcggcggaa agcatgaagt caaaagcgtc gatctgaacg
    28381 acctgattca tcagacgtcc cggaccatgg gccgcaccaa aaaggaaatc aacattcatc
    28441 accacttcga agacccgctc tggatgacgg aaatcgatcc ggcgcagatt gagcaggtgc
    28501 tggtgaatct gttcatcaac gcctggcagg ccatgcccgt cgggggggat ctgcacctgg
    28561 aaacgaagaa catgatcgtg gacgaaaatc gcttcaaaga cttcagcggc aaaggcggcc
    28621 gctatgtcaa gatctccgtg acggacaccg gcgtcggaat ggacgagaaa acccgggaaa
    28681 gaatttttga gccgtttttc accacccggg aaatggggag gggcaccggg ctgggcctgg
    28741 cgtccgttta cggcatcatt aaaaatcaca acggcttcat caacgtctat tccgaaaaag
    28801 ggcagggctc gaccttcaat atttatttgc cggcctcggc acaccagtcc caaaaccatc
    28861 aatgtccgga agaagcgtcc gccccggaga gatcctccga cagggaaacg atcctggtcg
    28921 tggacgatga agccatggtt ctggatgtgg ccggcgaaat gctgagaacc ttgaactacc
    28981 gggtaatcac cgttcccaac ggcgccgccg cgattgacct ctaccggcgg gagcaggagc
    29041 gcgttgatct ggtcattctg gatatggtca tgccggacat gaacgggtca gaggtcttcg
    29101 acgccatccg gcggatcaat ccgggcgtca gggttttact gtccagcggc tacagcctga
    29161 acggccaggc cggcaaaatc atggaacgcg gctgcagcgg cttcattcag aagccttttt
    29221 cgataagcga actggccgga aaattgcgga cgattctgga acagccgtcc ccgcggaaga
    29281 aggctccggc gtctggttcc atgtcctgaa cggcatccgg cgcgcgcccc tgacgatgcc
    29341 gcgtcaaagg caagcagaaa acgatgagaa aacattttaa gggacattta caacccgtgg
    29401 gcgacatcct ccttgccgca ttgaagaaaa gaggcctggc cggcaaactg gaggaaaatt
    29461 tcctgttcaa gctgtggccc aaagcggtcg gcgcgaaaat cgcctcgcaa acctgccccg
    29521 acggatggcg cgcgggcacc ctctttgtaa aaacgacctc ctccgtctgg gtgcagcagc
    29581 ttcatttcat gaaggaagac attcgaaaaa agatcaacga tctggcggga aaaacggtcg
    29641 tgcgggagat tcactttacc gtcggataca gtcccgcccg caagggcgcc gggcgccggg
    29701 ctgtcccgcc aaaactcatg ctgaaggaga gagacaaaag gatcatcgcc gaatgcaccg
    29761 aaacgctgaa agaccgcgaa ctggccgaaa tcgtccaacg ggtcatgcag gcggaaatca
    29821 gccgccgcag gcttcgggag gagcggcaag gtcgctgaaa agcttcttca tctccggcgt
    29881 ataggttttc tgcgcatcat aaatgtaaag cggcggctcc attttcagct cttcacggga
    29941 gccgtttctt ccttccgcca gcacaaacgc cgcgtcggat gacgcatcgg aaaacaccag
    30001 cttcattcgc ttgggctcga tgcccgttct gcgaaacagg ctgaccagct ccaccagccg
    30061 ggtcgccgga taaatcgtaa agacccttcc tttcggtttg agcagaacct tcgacgccgc
    30121 cagaaaatcc gccagcgatc cctgaagctc gtggcgggca atcgctttct ccgagagcgg
    30181 attcatcctg ccggaagaca tttttcgata gggcggattg aaaaggacat tgtcaaacga
    30241 atgggaggga aaggctctcc cgacgtcccg ggcgtcgccc gcaacaatcg cgacgcgctt
    30301 ttccaaacca ttttgctcga catttcggca ggccagcttt gccagccctt cctgaatttc
    30361 cagaccgatc cagcgggtca ggggatgccg tctggccatg accagcgcga gaattccgtt
    30421 gccgcagccc agatccagat tgacggaccg gggttttacc gaagcaaatc ccgccagcat
    30481 cagggaatcc agcgtaaacc ggtagcctct cgcgctctgg cggattttca aacttccgtc
    30541 caggatttcg tcaaccgttt cttcatccgt catttcgccg ctccccactg cgaggcatgg
    30601 agtcttttcc gggccgggct gcctgcgtca tgatccggtg caaggaccgc gggaatcccc
    30661 ctgccggctt cacgctccgg atttgaaaac gcgatccgct ttttcaattt tccgcggtat
    30721 caatcggtat atgcaatctc ttcatcctgt caatacataa aatcctatac cggcgatgcc
    30781 ttttccggcc atccggcgac cggagcagga cttctcctga attttccagg attttctatt
    30841 ccgccaacgc gatcatttcc caagattttc ttgacagacc ccacggcgtt tcctatactt
    30901 tgccgcgcca aaatacggat caaggatgcc tcgccatgcc aaaactttcc ctgaagcgaa
    30961 agatcatcgg tcttgccgtc gtggccgccg ttttgccggt tgtggtcatg atgatcctca
    31021 tgggatcttt caaaacctcc gtttcgaatc tggcctcgaa agagttgggc gacgtcgcca
    31081 tggccaacat gtcccagatt gccagggacg tgtacgggtt gtgcgaaacc gcaaacgacc
    31141 tgatgcagag aaaaatcaac aacgacctca atgtggccag aagcatcctg aagcagcagg
    31201 ggggcatcga tgcggccagg gaaaaggtcg cctgggacgc cgtcaaccaa ctgacgcagg
    31261 agactcagaa agtcgagctt cccaaactga cggcgggcgg cgtctggctg ggccagaacc
    31321 gcaatccgtc aacgcccacg ccggtggtcg atgacgtgaa gcgcctcgtc ggcatcacct
    31381 gcaccatttt tcagcgcatg aatgaaggcg gcgacatgct gagggtcgca accaacgtgg
    31441 aggacctcaa ccatcgccgg gccatcggca cctacattcc caaccttttg ccggacggaa
    31501 aagtcaaccc ggtgacggcg gcggtcctga agggacagcc ctatcgggga ctcgcctatg
    31561 tcgtcaacac ctggtatctg accgcctatg aacccatcac ggaccggagc ggaaaaatca
    31621 tcggcatgct ttacgtaggg gaaaagctgg aggccgtctc ttcgcttcgg aaagcgatta
    31681 tgaacatgaa ggtgggaaag ctgggctatg tgggcgtcgt cggcgggaaa aacgagcacc
    31741 ggggaagata cgtcatttcc aaagacggca agcgcgacgg ggaaaacatc tgggatcaga
    31801 aagatgtcca cggcaacctc accattcaga ccctgataac caaggcgctg gcccagccaa
    31861 agggcgaagc cttttatcat gaatacatct ggcaaaaccc cggcgatccc ggcccgagga
    31921 aaaagatatc gtccgtcatt tactttgagc ctttcgactg ggtgatcgtg gccggcacct
    31981 acgaggacga tttcttcggt cccatcggcc atgtgcacga tatgatcagg tccatcttcc
    32041 tgaaaatgat cctggcggga gcgctgatca tccttctgtc cgtgctgctg gccgtggtcc
    32101 tggcccggcg gttgacggcg cccctggaac tgacgacatc gctcgcccag aagatcgcca
    32161 ccggagacat tcagcaaacc cgggaagaac tggcaggggc cgagtcaacg cggatcattc
    32221 aatccggaga cgaaaccgga aaacttctcc aggccttcca ggccatgacg ggcaatctcg
    32281 actcgctcat cggccaggtt caccggtcgg gcatccaggt caccacatcc gccacggaaa
    32341 tcgccgcttc ggcgcgcgaa ctcgaagcga ccgtagccga gcaggcggct tccaccaccg
    32401 aagtgacggc caccagccgg gaaatagccg ccaccgcgga cgatctggcc cagaccatgg
    32461 acggggtcaa cgcatcgctc aatgaaacca tcgacatggc ccaggagggc cgcaaggacc
    32521 tctcccggat ggaaacggcc atgcgggaac tggtcaaggc tacctcctcc atcacctcga
    32581 agctgaccgt catcaatgac cgggccaaca ggatttccca cgtcgtcacg acgatcaaca
    32641 agatttccga ccagaccaat ctcctgtcgt taaacgccgc catcgaggcg gaaaaagccg
    32701 gtgaattcgg aaggggcttt tcggtcgtcg cccgcgagat cagccggctg gccgatcaga
    32761 cggccatcgc cacgcaggat atccagcagg tcgtctctga aatgcagacg tccgtcgcct
    32821 ccggcgtgat ggagatggac aaattcaacg acgaggtccg gcgcggcgtg gaaaccatgg
    32881 ccgcgcagag cgggcagctt gcccaaatca ttgatcaggt gcgcatccac gggccgcagt
    32941 tcaccgccgt caaggacggc atgtacggcc agtcccaggg cgcgcagcag atcagcgaag
    33001 ccatgggcca gttgtcgctg gcggcggagc agaccaaggc gtcgctgcac gaattcaagc
    33061 tggcaacgga gcagctcaac gaggccatcg cggggcttca gtcagaggtc tcccggttta
    33121 aaatcagctc ctgatcggga aacaacctat gttgctactt cagtttcagg caggcacgga
    33181 ccgttacggc ctcgatatcg gacagattat tgaagtatcc tccatggttt ccctgagggc
    33241 cgttcctcac gcgccgccgg aagtcgcggg aacgttcaat taccggggaa ccctcacccc
    33301 ggtggtggac ctgaccgttc ttttgaccgg agcttcctcc cgcccccgga tgagcacgcg
    33361 catcatcctg atcgcctacc agggagaaga cggccgttct cacatcctgg gcctgctggc
    33421 cgaagcggtg acggaaatga ttgactgcag gaaggaaagc ctccagcagc cggtggtgac
    33481 cgtcagcggc gcgccctatc tgggagacac gatgtttgac gagcgcggct ccattcaggt
    33541 cctggacatg gaacggctgc tcacccgcga cctgcttttc acccttttcc cgccggaggt
    33601 cgccaacgga tgaacgggcc ttccgccatc atggaattcc tcaccctttc cctgggcatg
    33661 gagccggact ccgcgggcaa agacgccgtc aaccgggccg ttgcagccgc catgcggcac
    33721 gagggcgttt cggatccgcg cgcgtttgaa aagctgttcg ggtcttctcc cgctgtgcgg
    33781 cagcggttga ttgacgccgt cgtcgtcgga gagacctggt tcttccgcga cagggggccg
    33841 tttctgtgcc tcgcccgtca tgccctgaac tggcacgagg cgcacccggg gaacgttttg
    33901 aatattctgt ccgctccctg cgcgaccggc gaagagcctt actccattgt catgacgctt
    33961 ctggccgccg gactgccgcc ttcatccttc acggtggacg gggtggatgt cagcgccctg
    34021 gccctcgaga aggcccgcct tgcctgctat tcgaaaagcg cattccgggg aaatatcgga
    34081 ccggacgtcg cccgcttttt cgagacaaca ccttccggcc gcaaagttgc cgatcccgtt
    34141 gtccgccggg tcgccttttc tctcgacaat ctggctttgc ccggaagcct tgccggacga
    34201 ggcccctatg cgatcatttt ctgcagaaac ctgctcatct atatgacccc tgaagcccgc
    34261 ctccggattt tcgaccggat ggaccggctc ctgatccccg gcgggctgct cttcaccggt
    34321 catacggaga caaatttctg gcatcagcgc ggctatctgc ccctcccgtg ggacagagcc
    34381 ttcgccctga ccaaacccgc ctcctccgcc tcgtttgccg ctgtccatac agggactccc
    34441 gcaaaaccga tttcacccaa aggcaaaccc ggtaaaatcg ccgtccttcc gacggacgcc
    34501 gccgcaaagg gaaaaacacc gatccatccg gacaccgacc cgccggagac tccctccggg
    34561 aaacgacgac aaccggcaaa gccaggagca gcaaatccag atgtcaaatt ccctccgaca
    34621 catgcacaga ttcaggaggc ccggcggctg gctgacgcag gagatacggc aggcgccgcc
    34681 cgtctctgcc gggaatacac gcgcaaattc ggaccggccg ccgaaaccta ttgcctgatg
    34741 ggaatcctct ctatggccgg gcaggactta aacggcgcgg aagaatgctt tttgaaggcc
    34801 ctctaccttg atcccaacca ctatgaaagc ctggttcaca tcagcctgat ctaccggcag
    34861 aaagggaagg aaatccaggc ggcgctttac cgggagcggg ccgagcggaa agcgaccgtc
    34921 aaggaatagg caaccgaaag aaggcggaac aaaaacgatg gcaaacgcag aaaaatactg
    34981 ctggaaggtg aaaggaatct acggcgacgg atcctgtccg gaactggcgc gcctgattca
    35041 ctgccgccgc tgtcccgttt tcgccacagc ggggagacag catctggacc gggaccttcc
    35101 gcttacatac ctcagggagc gggccgccgt gatggctctg cccaaagaga cgggcagcct
    35161 gggaaccctg tccgtaatgg tgttccgcct gtgcggcgag aagtttgccg tcaaaaccat
    35221 ctattttcag gaagcggcgg aaccgtcccc ggttcacacg ctgccgctga aggtcaatcg
    35281 gatttttcgg ggaatcgcca acattaacgg ggaactgctt ctgtgtctgt ccgtcgcgga
    35341 cctgctggat ctgacctccg aagcgcagga aagcaggcct gcgccggctt atgagagaat
    35401 gctggtggtc ggtcgtgaag ggcagcgctt cgtgtttccc gtcgaacaga ttctgggggt
    35461 ccatcggatt gccccggagg atctcggaga agttcccgcc acgctctcgc gcagcgccag
    35521 agcgctgacc cggggcatct tcctgctcaa tggccgcccg gtcgggcttc tggatgaaga
    35581 gcggctcttc cccgccatga caaggagtct gaacccatga ccacggacgc agcccccatc
    35641 gatcccctga tgctcgatct gttccgcgtg gagctggaaa acaacacccg gatgctggag
    35701 gccggtctgg tcacggcaga gcgcgaccag acgccggagc gcatcgaacc gctgatgcgc
    35761 gccgcccatt ccatcaaggg ggcagcccgg attgtggggt tgaacacagc cgtcaccctc
    35821 gcccatgcca tggaagacgt tctttcggcg gcacagagcg gaaaacggcc gctgacctcc
    35881 acagcggttg accggctgct caagggaaat gatttttttg cctctctttc ccgcctggac
    35941 attcaggagc tgcccgccgc tctggagcgg ggaacccagg aaattacagt tatggcaggc
    36001 gagctgagaa actttctctc gtcgcctcag gagaccacgc cggctcctac cgcggccgcg
    36061 caggcggaag aaaaaacggc cggggacgtc ccccaaaagg cgcaagacat taaagccgac
    36121 ccttcgccgg caccgcgccc cgccgagccc acacctcaaa agccgctccc ggcagccgtt
    36181 ttggaaaacg catgcccgga gccgcaaacc gcaccgccgg ccgtccgttc ccgccgttcg
    36241 caggatcagg gagacggagg cctggtgcgc gtccggtccg aaaacatgaa ccgtctgatg
    36301 gccctggcgg gcgaatgcct cgtgcaggcc caatccatca aaaccgtcta tcaaagcctc
    36361 ctggtcctca aaaaggaaat cttcgaaatc gaaaaacccc tgcgcgaatt cctggagcga
    36421 caggaagcaa acgagcagga caaaggagcg gaagacttac agcagacgct gtccggcgcg
    36481 gtctcggccc agcagatcgt ttcccgggaa attgtccggt tcgagcgctt ttcacgccgt
    36541 ctggaacatc tggcgcatcg cctttacaac gaagtcatca gcagccgcat ggtgcctttc
    36601 tcggacggcc tccacggttt cccccgtctg gtccgggatc tggcccggga aacggggaaa
    36661 caggtccgct tcgaaattgc cggagccgcc acgcccgtcg atcgggatat tctggagcgt
    36721 ctggaggcgc ccctgtccca tttgatccgc aacgccgtcg atcacggcat ggaaacaccc
    36781 gaagaacgca aagcggcggg caaacctgcc gaaggaaccc tcgttctgga agcgcgccac
    36841 gtcgcgggaa tgctgaacat ctcgctcacc gacgacggcc ggggaatcaa tcccgaggat
    36901 ttaaggggca aggtggttga aaaaggctat gtgtcgcagg acatggcggg aagcttaagc
    36961 cacgcggagc tcttcgagtt tctgtttctg ccggggtttt ccacggcccg caaggtaacg
    37021 gaaatttccg gccggggcgt cggcctcgac gtcgttttca acatggccca tgaagtgggc
    37081 ggaaccgtgc gcgtggatgc cgacgccggc cggggcgccc gctttcacct gcagctgccg
    37141 ctgacgcttt ccgtgctcag aaccctgctt ttgtcgattg ccggcgaagc ctatgctctg
    37201 cccctttccc gcatcgaccg ggttttgtcc ctggcgccgg ccgacatccg ggaaatcgaa
    37261 gaccgcccct actgcacact ggacggggag catgtgggca ttctcgacgc gcgccagctt
    37321 ttcgggctgc cgcaggcttc caccccgcct gcgggatttt ccgtcgtcat catcagcgac
    37381 cggctgaacc gctacgggct ggtggttgac cgcgtgctgg gcgaggaaaa tctcgtggtc
    37441 cggccgctgg acagccgcct cggcaaggtc cccaatgtca gcgccggcgc cattctggac
    37501 gacggttcgc cggtggtcat tttcgatgtg gacgatctgg tccgttccat tcaccagttg
    37561 ctgacgaccg gcaagcttca gaaaatcggc cggggccggc aggctctgaa gacgggcaaa
    37621 aagcgcgttc tggtggtgga cgactccctg accgtgcgcg aagtggagcg cagacttctg
    37681 gaaaacgccg gatacgaggt caccaccgcc attgacggcg tggacggctg gaacaagctg
    37741 acgtcgcacg cctttgaact gctcgtttcc gacgtggaca tgccgcgcat gaacggcatc
    37801 gatctcgtcc ggaccgtcaa aaccgatccg cggcttaagg acatgccggt gatgatcgtc
    37861 tcctacaaag accgggagga ggatcggctg cgcggcctcg aagccggcgc caactactac
    37921 ctgaccaagg gcagcttcca tgacgaaagc ctgctcgatg ccgtccgcga cctgatcgga
    37981 ggacccgaat gacgctcaaa atcgccattg cgcatcacga cgagaacgcc ctgaagatcc
    38041 tgcggcagat cattgccccg gataccggct gcgcggtcat ctggtccaca ggccgcggga
    38101 gcgatgtcat ccggcaatgc cgtcatgact tgcccgatat cctgctcatc gacctggcgc
    38161 ttgccgacgt cagcggcgcg aggctgatct gcgacgtgat gaaagaaacc ccctgcgcga
    38221 ttctggtcct gtccaacgcc agggaacagc aggcgggaaa agtgtttgag gccatggggt
    38281 gcggggcgct ggatgtcatt tccacgcctg tgcttcattc caacgggttt ctggaagggg
    38341 cggacgtcct catcaaaaaa atcgacacca tggccaggct tctgggaagc gcttcccgga
    38401 cgacaaaacc ggccgcggct gtccggcaga cggaaaaaga gcctcagccg cagctcgtcg
    38461 ccatcggttc ttcgacggga ggccccaagg ccctggccac gatcctcgcc ggcctccccc
    38521 gggaatttcc cgccgccatc gtcatctgcc agcatgtcga tctgcagttc gccggcggcc
    38581 tcgcctcctg gctgaactca caaacgccgc tttccgtagc cgttgcccgg gaggggatgc
    38641 gggtccgacc cggcgtcgtc ctggtggccc aaaccaacga ccacctcgtc ctcggaccgg
    38701 acggcgcgct tcactacacg gagcaccccc gcaattatcc ctatcgcccg tccgtcaacg
    38761 cgctttttga aagcctccgg caatactggc cgcaaaatga cgtagcggtg ctcctcaccg
    38821 gcatggggcg ggacggcgga gagggactgt cggctctctt caaggccggc tggcacacga
    38881 tcgtccagga cgaaaaaacg tccatcgtct atggcatgcc ggcggcggcc gtggaactgg
    38941 gcggcgcttc ggaaatcctg ccgctggacc aaatcgccgg cgccattgcc aggaaaatta
    39001 tcaaccgcaa aggaacctca ttatgaccga aaaagaaaat ttaaacccgg ccccgcccgg
    39061 cagccacgat ccggcaagca cactgaccgc ccataaaatc aaggtcctgc tcatcgacga
    39121 ccaggcgatt gtcggcgcgg cggtgaaaaa aatgctcgaa ccggagacgg acattgtctt
    39181 ttcgttctgc caggatccca cgcaggccat tccgaccgcc gccgcctttc agccgacggt
    39241 gatccttcag gacctgatca tgccggagat tgacggtctg acgctggtga aattcttccg
    39301 cgtccatccc cagctcaagg acgtgccgct gatcgtttta tcctccaagg aggaagcggt
    39361 gacaaaggcg gacgccttct cgctcggggc caacgactac ctggtgaagc ttcccgaccg
    39421 catagagctc atcgcccgga tccgctacca ttcccgcggc tacatcaacc tgctgcagcg
    39481 aaacgaagcg tatgccgcgc tgctcgccag ccggcaggcc ctggccgcgg aactggccca
    39541 ggcggccgat tacgtggtat cgcttatgcc gccccctctt tccgatccga ccatcacgac
    39601 ggcctggcgc tttttcccca gcgcccagct cggcggcgac agcttcggct accactggct
    39661 cgatgaaaac acgttcgcca tctatctgtt ggacgtctgc gggcacgggg tcggctcggc
    39721 gctgctttcc gtttccgcct tcaacgccat ccgctcccag acgctgcccg acacggattt
    39781 ccattccccg gaccgggtgc ttgccgcgct caacaacgcg tttcagatgg agcaccacaa
    39841 caacctttat ttcaccatct ggtacggcgt tttcgaccgc cggacccggg aacttcgctt
    39901 tgccagcggc ggccatcccc cggcttttct catttccaaa gagggaaccc tgaccaaact
    39961 catgacgaac aacctgttca tcggcggcct tccggaaagc gcctaccagg acagcgcgat
    40021 caccgttgcc cccggagcga atctttacgt cttttccgac ggcgtttacg aagtggatcc
    40081 gcccgaaggc agcatgtgga cgctggatga actgggagac tatctgctgc agcacaaaat
    40141 ggaagcgggc gaagatattg acgccctgta ccggaaaatg caggactacc acggccagaa
    40201 aattctggaa gacgactttt ccatgctggc cgttcatttc acataggacg cgggaagcag
    40261 aagaagaccg ggcgccgggc cgtcccggaa tcggctcctg tgccgaacag cttcgagagc
    40321 tttgactttc tcacgaaacc cggtaatttg tcattcctgt gaaaacggga atccagtctt
    40381 ttcgaaacct tacggatccc cgcctatgcg ggaatggcag aaaaaggacg tttttcaaag
    40441 cgtctctctt tatcggcaaa gcgatttcag attgttgatc acgacaaccg cgtacagcac
    40501 gacgataaaa cccagcgtat aaacggcaaa aggcccgcca tacgcatagg caacccagca
    40561 gaccgccacg ccgaagcttt tggccagcgc cagcccccgg acgacgccga gcctgtcaaa
    40621 cagccggcgc aaaattctgt tccattccct cacaccctgt ttttgaaggg caagaaaggt
    40681 cgtcaccccg tcgccgatct ggagggcgat ggccaggcct aaaagcattt cattcacggg
    40741 ctttcatcct tcgccatgaa agctttgcaa aaccgcatca aagcgctgat tttgtcacgc
    40801 gtgacctcgt gtgaccttca ggttatcagg tcataccggc gcaggccggt atccagtata
    40861 atcattctgg attccggttt ttgccagcac cgctgaagcg acgcactaca ctgaccatgg
    40921 cgccaatccg atgtagcgcg tggttttaac cccgctaaaa aaccgcgcgg ctgaagccac
    40981 gcactacatt gaacaggggt cgcgaggaag gacggctttt gaccattctg acacaacctc
    41041 tatttgagga gcaaaaagaa gagttcacct ttttccttga ggctgccggc gatggcctgg
    41101 gcttcggggc cgaacccgca aaacagatcg acccgccccg gaccccgaat ggcggatccc
    41161 ttgtcctgat tcaggacaaa gcgcgaaaat tcgacgcggc ggaccacccg gtaatcctcg
    41221 tccagaaccg gttttcgcaa acggatgaac gccagcgcgc cctgcgggaa atacgccggg
    41281 tccgtagcaa tcgaccggtg aggcgtcacc ggctgcccca gcgagccgac cggttcgctc
    41341 tccacgaagc ggaaaaaaat gtaacgttcg ttgtagctca aaatttcata aatttctttt
    41401 tcgcttttgc ctttgagcca ggctttgaat ccacggtagg aggcgttgcc cgggtcgatt
    41461 ttactttcgt tcagcaggtt ctgcgtcgcg gagcgaaaag gcctgccgtt gttctgagcg
    41521 tagctgacgg tcagcattct gccgtcttcc agccggatct ttcccgatcc ctgggtgtgc
    41581 agggaattca gctcgacggg gtcggacacc cagatcagct ccagccccct gccctgcaat
    41641 acgccgtcca catcgatttc ccggcgggag taatagggaa ccagccggcc gttctccctt
    41701 cggctgatcc tggtttcatg tttggatatc ttttccacaa caatgtccgg cggagggcgg
    41761 tagagcggat atttgtactt ctccgtccgc gtcagagagc cttccaaaag cggctcatag
    41821 tagccggtaa acagaacggc gccgtccccg ctttcgcccg ccgcccggag gaggagaaat
    41881 tcttcggcaa tccgccggtt cttctcacca gaaggaatat cggacaccag aattttgcga
    41941 aaagcgatca gcgtttcctt catctgctgc gcgctcacct gacggtccgc aatccggaaa
    42001 atctgattct ttcctgaacc gtcgtaaaag agcaaactgc ggtcgatggc cgtgagaaga
    42061 gattcggcat cgaggtcgtc aaccaaatcc agttgccggg catccgctgg aaccagcgcc
    42121 tcgcctggcc ggggcgcttc cggcctgaac gcctcttttt tcgcgcagcc ggccaaaagg
    42181 accaggaccg ccagcatcag gagcaaaccg attttcagaa gtttcatagt tcgatgatca
    42241 tcgcggtttc gtcgcagtag tccggaaatt tggggcagcg aaagcagtcc gaccagacct
    42301 tttccggcag aatggagcgg tccacttcct tgaagcccag tccggcaaag aattctttct
    42361 tgtacgtcag ggtgaagatg cggaacaact ccagggtaat ggcgtcggaa atgcagaatt
    42421 cgaccagctt tcgcccgatg cctcttctcc ggaaggcttc gtccacgtaa agggaacgga
    42481 tttccgccag attttcccag atgatgttca tcgcgcagat tcccacgatc ttctgttctt
    42541 cttcgtcgaa ataaacgatg aagtcccgaa cggaattgta aatatccatc agggaacgag
    42601 gcagcatctc ccctttcgcc gccgacacat tgatcagccg gtgaatggtc ttgacgtcac
    42661 cgatgcgcgc ttttcttagc attcctgctc cttcctgcgg cggcccccgc ggcggccagc
    42721 agttcccgcg catgatccct cgcggtttcg gtcacatcca cgccgcccag catccgtccg
    42781 atttcctcga ttttctgctc gtcatcgatt ttttccacga ccgtggacgt ccggccgccg
    42841 acgattttct tgctgacccg cagatgcggg ccgccgaagc tggcgatctg cggcaggtgc
    42901 gtgatgcaga tgacctgatg atgcgccgac agggtcttca attttcgtcc gacgatctcc
    42961 gccgtggcgc cgccgatgcc ggagtccact tcatcgaaca cgacggtgtc caccgaaccg
    43021 atccgcgtca gaacattctt cagcgccagc acgatgcgcg acaactcgcc gccggaagcg
    43081 attttatgga gcgctttcgg ctcttccccg gcattggccg tcagataaaa ttcgatttca
    43141 tcgccgccct tcggtccgca ggctgtttca tcctccggac agcgccgcag aaatttcacg
    43201 gcaaaggagg cgtgcggcat gttcagatcg tggatttccc ggtccaccga gcgctccagg
    43261 cttcgcgcgg cctgctgcct catcgtcgaa agcccgagcg ccttttcccg aagctccccc
    43321 gccgccgcgt ctttttcctt tttcaagcgc gcaagctctt cttccgcgcc ggagacgctc
    43381 tgcagctctt cttccatttc ccgctttctg gccagcaccg cttcgagcgt tccgccatgc
    43441 ttgcgcttga gccggcccag cgcgtcctgg cgttcatcga tcgccgccag tcgctcggga
    43501 tcaaatacca gttttttttg gtagtcgcgc agcgcgagcg ccgcctcctg caaaatgacg
    43561 tagcttcctt ccacgtcggc cggccggaga ttcaatcccg gatcgatctt ctgaatttcc
    43621 cgaatctgcg agagggcctc cttaagctgt tcgccgacgg agcccgaatc cgcataaagc
    43681 agcgcgtagg cccgatccgc ccgatccgcg agcgcgcgcg cgttggccag cacctttcgt
    43741 tcatcggcaa gctgtgcatc ttcgcccgcg cggggattca gacttttgat ttcctcaagc
    43801 tgaaaccgga tccagtcggc tttttccgcg cttcttcgcc gcagctcctc cagctttgcg
    43861 atgcggccga ggcactgctg aaatcggtcg taaaccgccg cgtattccct gcgggcgtcc
    43921 agaatgccgc cgaaggcatc gagaatgtcg atgtgatttt ccgcgaagag aatcacctga
    43981 tgttcatgct gtccgcagat attgatgagc gattcgctga tcgcggccag attcgccagc
    44041 gtcgccatct gtccgttgat ttgcgccttg ttcttgccgg agcgcgaaat gaccctgcgg
    44101 atcacgaggt cgtcgccggc ggcaaacccc atctgggcca gcttttcctg aagcgcccgg
    44161 ttgccctgaa tatcaaagag cgcctccacg gtcgccgtat ccgtttgcgt ccggatcatg
    44221 tccgccgctg cgcggtcgcc caagagcaga ctgaccgcgc cgatcagaat ggacttgccc
    44281 gcgccggtct ctcccgaaat cacgttcaga ccttcatcaa acgaaacgcg cagttcgtcg
    44341 atgatggcaa aattggcaat gctcagatca cgaagcattt ttccttttcc cagcgcctga
    44401 aggcagactg ccccagccca gtttggaacg cagaatctcc ccgtaaccgc ggtgcggtga
    44461 aagcaccagc cttgtgtaaa attttgattt tcggatggtc atgacgtcgc cggaactgat
    44521 ccggtaggat tcctgtccgt ccagcgtcac ggtggcgccg ctttccttgg accaaagcgt
    44581 aatctgcagg tcggaatttt cggggaaaat gatcggccgg ttggtcagcg tgtggggaca
    44641 gatcggattg atgatgatca gttcttttcc gggatagacg atcggaccgc ccgcggaaag
    44701 cgaataggcc gtcgaacccg tgggggttga aacgatcagg ccgtcggatt tgtaggttgt
    44761 gagataatgg ccgctcacgg acgtttccag ctcgacaatg cgggacaggt tgccgcggtt
    44821 gatcaccacg tcattcaaaa cgatgcccga cgcgatggtt ttgttgcccc ggcggatttt
    44881 cacgtccagc atcatccgtt tttcggtcag gaaatcgccc tggagaatca gctcgaatgc
    44941 cgcgtacgtc tcgttgagat tgacctccgt gagatagccg aacgtcccca ggttgatgcc
    45001 gacgatgggc acggggtgct gcgcgacatt gcgggcggtt cgcagcatcg tgccgtcgcc
    45061 gcccagcacg gcgatcagat ccgctctggc ggccagatct tccagtttga agcctccact
    45121 cccgccaacg gcggccgcaa tgttggcttc cagagcaacg cggattcccc tggcttccag
    45181 ccattttttg agcgcgccgg tgaatccggc gatgttttcc ttctcgatgt tggcgacaat
    45241 gccgactttc ttaattttca tggtgacggg ctccaaaaaa gacgaggttt cgtaacataa
    45301 aaaaatgtcg ctgtctatgg ataatttttt ggccaatcga cgcaaactct ttgatttata
    45361 aagatattca caaagcggaa tgtcttgaca ggacaacagc ccataaggta cggtgcgcat
    45421 gctcacgcgg gggtaaaacc agcgggacac cataaaacca tgccaactat gaccttggcc
    45481 ttcttggagt aaaagatatg gggttaatca ggggcgcctt tacattcatg cagttttctg
    45541 tggacggcaa actgccgcag gcctttacaa catttgcccg cagccggatt cagggcaacg
    45601 cgtttcggga ggcgcgcagc tccaccgagg aaaaacggat gggctgggtg tccgcgacgg
    45661 acgtcctgga cgccgatttt gaaaacacac ccttctcgct gggcgattat ctgatcttct
    45721 ccctgcgcat cgaccgcaag gtgatcccgc cgaagttgat gaaagtccgc ctgatggaag
    45781 agcagcggag gttcctgtcc gagcatcagc aaacccgcat cggcaaagcc atgaatgaag
    45841 ggctcaaaga aaaggtccgg ctggagatta tgaccaaaag cgatcccgtg ccgtcttttt
    45901 acgatgtcct gtgggccgtg gggcaaaaca aggtttattt ttcctccctg tccgacaagg
    45961 tcgcggatga ttttgtcgat tacttcaaaa aaactttttc gctgggcttg aagcgaatcg
    46021 tcccccatga acatccgctg gcgctgaaaa acaaggcaaa aggcgacgcc cccacggaag
    46081 attttaccct catcggacgg gaatttctca cctggctgtg gttcaaatcc gaagaaagaa
    46141 acggcgccat cgcccttgcc aaaacggacg aggtgcagct gcaactgctc aaacggatcg
    46201 ctctggaggc gggcgaggga gaatactcgc agggggttgt ctgctccgga ctgcacgccg
    46261 aactcaagga aggcaaggag gccatccgcc aggggaaaaa agtgaaggag gcggggattt
    46321 tgctccatcg cgaccgggat gaatgggaat tcaatttcaa ggccgacgcc ttttacttcc
    46381 agtcgctcag gatgccggcc gccgacccgc cggaaacgca ggaagacgcg gaaggcgttc
    46441 ttttggagcg catctaccgc attgaaaacg cggtcaggac catcgaccgg ctttacgaat
    46501 cttttctaac gcttcgcttt tctccggagt ggacgaaaaa cgaaaaaccg cgcctggccg
    46561 cgtggctgag gaaggagcag gagtaggtca tggctcatga gtcatgaccg atggctcatc
    46621 acccgccgct gaagcaatga cggttcacga ctcttcagtt gacaacattt tacaagaatg
    46681 ccagactgct tataatgaat ggatattggc gcaccttttc aatgagtcat tttgaacgca
    46741 atttcccgga cgcgtaaaca ggggtcgcaa t
//
LOCUS       DAOSRM010000006        37033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1254_length_37033_cov_102.326518, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000006 DAOSRM010000000
VERSION     DAOSRM010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 37033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37033
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_37033_cov_102.326518"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PLA58_01480"
     CDS             complement(<1..1121)
                     /locus_tag="PLA58_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOR61496.1"
                     /translation="MAKILVLDDDQGMREFMEIMLAKEGYDVTCTGKPEKAISLCRKT
                     AYDLVITDLKMPKIDGIEFLKTIKEHRPETVVILITAYASGETAVNAMKEGAYDYVEK
                     SGNIDELKQVVRQALLKQGSLSEGQTLKENPAAQTSFCGMIGTSREMAKVFATIRKVA
                     DTPANILILGESGTGKELVARAIHENSSRASMPFMAINSGGIPENLLESELFGYMKGS
                     FTGAYGDRAGLFELARGGTVFLDEIGELPPVLQVKLLRVVQQKTFRRIGGEKDITVDV
                     RIISATNQNLKEKVARGAFREDLYFRLNVIPIHMPPLRERKEDIPLLTRHFIEKYARE
                     FGKEVRTISSYAMELLMEYPFPGNIRELENIIERGVAMET"
     gene            complement(1140..2786)
                     /locus_tag="PLA58_01485"
     CDS             complement(1140..2786)
                     /locus_tag="PLA58_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR61497.1"
                     /translation="MEYDERTWRRLKLLIVSRVVIISAFLLVTLFLGAVKPIIFISRD
                     VLHAIYAVIVAMFILSVIYTLLLRKKKYFQINIYFQLAVDIALVSFLVYLTGSIGSNY
                     SLLYTLVIIYAAIFLGRRGALIFASACSIAYGLVLDLEYFGVIPTFYDTGRDYTLEAG
                     DVFIRIAVHILSFYILAFLASFVVEQEKKARSLLAERESAFDQLDLLFRSIIESVDTG
                     IMTTTLQGRIKTFNRSAEKITGRPLRSVENRQIEDVFPVFAPLFSAGGEPLMKSKGEI
                     RINGREGTPMYLGYALSPLKDRKNEQIGHILIFQDMTEIRRMEENLEKSRRLALIGEM
                     AAGLAHEMRNPLASITGSIQLMAQTPDLDETDKRLMQIILRGKDRLESFVRDFLLLAR
                     PVPEARQSIRLNDVAEEVVESVKMSADWTEDIRLEKKISGENRALANEEQIRQILQNL
                     LTNALQSMDGKGQIGLEIRTVRLEDEKEYAEIKVTDDGCGIEEKDQKKIFEPFFTNKE
                     RGTGLGLAIVNRIVDGYRGRIAIQSAVHEGTTITVWLPCR"
     gene            3002..5533
                     /locus_tag="PLA58_01490"
     CDS             3002..5533
                     /locus_tag="PLA58_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOR61498.1"
                     /translation="MRRPLWYPLIALMTGILIGDRFVLAPEVLLAGMLPVLALLLLCL
                     RRRWNAAALIALLLLTLTAGMLNIQRQPFLARQEKHVLHLADQGKMTLEGVVLSTEQA
                     FPGKSTLIVLCRRVLQNQTPAPVTGKIRVAIPSDLSFQYGDFIRFHTKIKKIQGFHNP
                     GSFDYERSQNRRGLYVSGFVSDRAGIVLIRPDTASGLKLKLERFRLYLKRLLEVHAAS
                     PQREILQAMTIGDQKAIPQDVRDQFAKTGTSHILSISGLHVGIVAASAFFLMLLAFKS
                     SEYCMLKFNIIKTATAAAIVPVLIYALVAGMGTPVLRSTLMTLAFLAALLIGRPKDLY
                     NILAGAALIILIASPEALFEISFQLSFSAVLAILYGVEKLGKRQIPLPSAAPRWIAAG
                     VNRLYLFLLVSAAATLGTLPIIAFYFNRVSAVALIANLVAVPLLGILALIPAMMFILT
                     SVFSAALAGFFISIASFFTGIAVSIIEELALLPWSSLSFIKPNALEIALFYAFMYFLI
                     EALTTDRPKDLQDVPARRPLWIKTGLLVCAALILADAAFLVLKDQYSSHLKITAIDVG
                     QGSSALVRMPGGVNMLIDGGGFHDSSFDMGRAVIAPFLYAKRIRKVDIAVLTHPHPDH
                     LQGLIHIVNAFDVREVWCAGLTADNDLYRLWEKTVAERGVKVRTLSAQSPPEVVSGVT
                     FQFLWPLRPPPPGVQGMSYQETNDASLVIKMTYGARRFLVTGDISAAIEAFLTASGKD
                     LKSDVLFVPHHGSLSSSSPDFLRVVSCRYAVISAGKNNVFRHPHPAVLERYRSAGAAI
                     FRTDRDGAVSIDSDGQSLSVTPWIKKPDPDFVRRRAPNISLPTTG"
     gene            5571..6833
                     /gene="hisS"
                     /locus_tag="PLA58_01495"
     CDS             5571..6833
                     /gene="hisS"
                     /locus_tag="PLA58_01495"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOR61499.1"
                     /translation="MEKITAIKGIKDILPKDSPVWHRVELTARRIFECFGFCEIRVPI
                     MEKTSLFQRGIGETTDIVEKEMYTFRDRDDELLTLRPEATASVIRAYIEHNLSASDPV
                     TRLYTMGPMFRRERPQKGRFRQFHQIDVELFGDESPASDAEVIFMLMHFLQSLNIENL
                     SLEINSLGCKACRPVFSRAVVDYLKGQESSLCPDCRRRMDVNPLRVFDCKVESCGSVI
                     AGAPRILDFLCADCLEHFSRVKSYLNELKLTYEINPRMVRGLDYYTRTAFEVKSGELG
                     AQNSLAGGGRYDGLVRFLGGPDVPGIGFGVGMERLIACLPPAGEVSSSADIFIAALGE
                     RAQKIAFTLTQELRRRGICAAMDYSGKSLKSQMKRADKLNSAYTLIFGDREIEENCVE
                     LRDMNSKNQQRLPLDHLLEAVTDLLKAR"
     gene            6841..8613
                     /gene="aspS"
                     /locus_tag="PLA58_01500"
     CDS             6841..8613
                     /gene="aspS"
                     /locus_tag="PLA58_01500"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOR61500.1"
                     /translation="MSRFITIEKRTHYCGSLTGADEGRQVVLMGWVHRRRDHGGVIFV
                     DFRDREGIVQIVFNPEAGESVHGEAHKIRSEYVLAVKGRVCRRPAGMENPALSTGQVE
                     VVISELEILNESKTPPFSFDDEDISENVRLKYRYLDLRRPAIQKNLFLRSRLAIATRQ
                     YFNDNGFIEVETPFLTKSTPEGARDYLVPSRISKGMFYALPQSPQIFKQLLMVSGFDR
                     YFQIVKCFRDEDLRADRQPEFTQIDVEMSFVSEEDILRMMEGLMAAIFKACRNIDLPL
                     PFPRLTYAEAVSRYGKDNPDVRFGMEITDLTAIVKDSGFKLFAEVAASGGVVKAVKAD
                     QASSLSRKDLDALSDFVAIYGAKGLAWAKVNAADWTSPIFKFLKPEETAAIGRAMDAK
                     EGDILFFVADTPKTACDALGNLRLHLGKKLNRIDPDALAFTWITEFPLMEYSESEKRF
                     VSTHHPFTSPFLEDIPLMTTDPGKVRARAYDLVLNGSEIGGGSIRIHRKDIQAQVFSA
                     LGLSDEEARLKFGFLLDALEYGAPPHGGLAFGLDRLVMIMTGAESIRDVIAFPKTQKA
                     ADLMSDAPSKVSIEQLMELSLKIV"
     gene            complement(8610..9869)
                     /locus_tag="PLA58_01505"
     CDS             complement(8610..9869)
                     /locus_tag="PLA58_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR61501.1"
                     /translation="MKIRQKGGTFDLIQQMAARCLKCKVPQCRKHCPVSTPIPDVTRL
                     VEEGKEQEAAQILFANNPLSAVCSIVCPHERNCFGHCVLGKKSEPVDFFKIEQFLSWT
                     FLKTYEPPVMEKNGARIAVIGSGPAGITMSILLSLMGYRVTLMEARDRIGGVLRYGIP
                     GFRLPREIVDRYDPILRQLGVQFKPNTFIGSSITLDDMFIDGYKAVFIAVGTAKPNKL
                     GLLGETLGNVFYAIDYLKSPEAYRLGRNVVVVGAGNVALDAARIAVRSIEPGGAVTVI
                     NNLLEEEIFGNRQEIDDAVREGIQFVHLLQALKLEEDGVKCVAVRAEKTESGTVYEED
                     FSRTQKIPADSIIVAIGQGPQGAVMADSNVSKTHRGLVAADENGHTSSPGVFAAGDIV
                     TGPKTVVEAVAFAKKVALEIDRYCREN"
     gene            10039..10230
                     /locus_tag="PLA58_01510"
     CDS             10039..10230
                     /locus_tag="PLA58_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61502.1"
                     /translation="MNSKTFSEKDKQMAKKCVECPVCRQARKHQKGLAFWFVKNIEQG
                     ICPYCAAYERVYGKKAHEA"
     gene            10285..10785
                     /locus_tag="PLA58_01515"
     CDS             10285..10785
                     /locus_tag="PLA58_01515"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOR61503.1"
                     /translation="MKDFKYMSMADQAMDKIRKGAFLTVAAEGELNTMTIGWAMFGVV
                     WQKQVLMVAVRRTRHTFGLMEKASDFTVTVPSGDMSRETAFCGTKSGRDVDKFRMCNL
                     KTANGRHVASPIIQTPGIHFECRTVYKSALNPANLDSGLDQTIYPGKDYHTLYFGEVL
                     ACYETE"
     gene            10797..11258
                     /locus_tag="PLA58_01520"
     CDS             10797..11258
                     /locus_tag="PLA58_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61504.1"
                     /translation="MSGKRNDLLPGNATEAQGLLIKAFHEKCGDEILPVIDDILGRQG
                     RALGLKVKNKLPDCRLSAVAAAFTKNFDPSTVRVVSLSDEKFHIQGSHCPFGLENTSR
                     QLCEAVMAIDREYFFTATDGQTTLEILKTRAAGDPICDTIYRAAAYDADKA"
     gene            complement(11280..13247)
                     /locus_tag="PLA58_01525"
     CDS             complement(11280..13247)
                     /locus_tag="PLA58_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOR61505.1"
                     /translation="MIAYERELNPEQYRVVTEKGGPLLVLAGAGSGKTRTLTYRVARL
                     LEDGVPPERILLATFTNKAARSMLCRVESLVGRGAGNILGGTFHSIAHRLLRSYASRL
                     DYSHRFSIVDTEDARQIIRQAMDDLHIDASLSKFPKNTILAEIISLAVNTESTLEDVL
                     ESRYPFFLHLSPEIHSIARRYAQKKKELQVMDFDDLLANGRRLLREHPDVLQTLSQRF
                     QYVLVDEYQDTNVLQAEMVDLIASGHRNLMAVGDDSQSIYSFRGADFKNILRFPERYP
                     DCKIFKLETNYRSTPEILHLANLSIHQNENQFPKALKAVRGRGTRPILVSAQNVLRQA
                     DFVAQRIAELARAGVAFGDMAVLYRAHYHSMEVQMEMVRRDIPFDIRSGIRFFEQAHV
                     KDVTSYMRVLVRPDDELAWRRLMMMYPKIGKITFEKIWARLSRQENPLEALLSESFLK
                     TAPKPAQAGLEKCRKTLLLLLSLEAADRTPGKIIDLLLNQGGYRTHLQDTYSDATGRE
                     EDLMQLGNFSTRFETMDEFLNELALLTNMSKEEDIEERQADRVILSTIHQAKGLEWSY
                     VFIIGCADGMIPLQRALREPGGEEEERRLFYVAVTRAKDQLYLSYPVLDYSRSAGTLT
                     LSSSRFVDEISPLSPQDEQRPFEQWALFDDV"
     gene            complement(13263..13910)
                     /locus_tag="PLA58_01530"
     CDS             complement(13263..13910)
                     /locus_tag="PLA58_01530"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOR61506.1"
                     /translation="MRIILLGAPGAGKGTVAKLLTEFDGSVQISTGDILRTAVKEGTA
                     LGRKAKEYMDRGDLAPDSLIMDIMEARLQEPDCQTGFILDGFPRTIPQAQDLKKLLAK
                     LNIALDFVANLDVPRDVILDRLTTRRTCCNPACQAIYNVKSNPPREDGTCTLCDHQTV
                     QRDDETEEAISNRLETYNAKTAPLIGFYEKEGLLKTFLSLSSKDTVAEIKKALGA"
     gene            complement(14046..14870)
                     /locus_tag="PLA58_01535"
     CDS             complement(14046..14870)
                     /locus_tag="PLA58_01535"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOR61507.1"
                     /translation="MIRNIEKIFVALIFISICVLRPGEAGAENVLPDKNPFSILMQTK
                     APISSVAVQKIIPHDANAFTQGLIIMDGSLCESTGLLGRSTLACKDIATGKVLRQIQL
                     PTEFFGEGIALFKNRIYLLTWQNQTALVYDAASLKEIRRIQYEGEGWGLASDGKSLLM
                     SDGSSTIVFRDPESFKIRRRIHVSDGDRPVSGLNELEFVRGEIWANIYLEDIIARISP
                     HTGRITGWIDLSPLRSYLPANARVDVLNGIAYDGKRNRMFVTGKYWPHLFEIQLSP"
     gene            complement(14942..15631)
                     /gene="tsaB"
                     /locus_tag="PLA58_01540"
     CDS             complement(14942..15631)
                     /gene="tsaB"
                     /locus_tag="PLA58_01540"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOR61508.1"
                     /translation="MLTLAFDTSSKTLSVALLRNRTVLHHVILHREQNHSEVLLPAID
                     EACRLSNVTVRDMDLFACTLGPGSFTGLRIGVSTLKGLMLATGKPAAGVSSLAALALN
                     VEEPDAVIGSMMDAGRGQVYLAYYRCGKDGILHPVGEPQAQSPRSVEDPFSEPVIYVG
                     DGVVRYAGMIPPLTSPKNKMAPAEQQFVSAAAVARLALEKYFRGDLLDPARCAPVYLR
                     SVDALPARSGG"
     gene            complement(15633..16724)
                     /gene="rseP"
                     /locus_tag="PLA58_01545"
     CDS             complement(15633..16724)
                     /gene="rseP"
                     /locus_tag="PLA58_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOR61509.1"
                     /translation="MVTFISFIVLLGILIFAHELGHFLAARAGGVGVLKFSLGFGPKI
                     FGKKIGETEYVLSWIPLGGFVKLLGESGTEELPPEDAKRSFYKQPRWKRLLIVLAGPV
                     FNFLLAIVIFFIVYLYGLPNLLPVIGELSPDSAALQAGILKDDKILSINGRPIKFWDE
                     IRPVIKESGGRNLSILVQRGSEEKTFAVKPKLSRAKNIFGEEESTYLIGISPAGRTVI
                     ERKNPLAAAVSAVDKTWEISKLTVISVVKMLEGVLSPRTLGGPIFIAQVAGDQVKEGI
                     IPFVLFMAILSINLGVINLFPIPVLDGGHIFFYLIEMATRREIPVKIKEWAQQIGFVV
                     LLMLMLFVIMIDIERLNIKPINDVLKFFK"
     gene            complement(16729..17895)
                     /locus_tag="PLA58_01550"
     CDS             complement(16729..17895)
                     /locus_tag="PLA58_01550"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOR61510.1"
                     /translation="MKKITILGSTGSIGASALDVIRKNPKKFRVAALAAGRNVALLAR
                     QTLAFHPEAVSVQTPEDAKHLRDLLGSRNPVRILCGEAGAQDVAAHRSADMVLSAISG
                     AAGLKPTLAAIEAGKDLALANKETMVIAGHLVTKKAKQKKIRVLPVDSEHSAIFQCLA
                     GQKPKNVKRIILTASGGPFYSFSRADLKKVTPAQALRHPRWKMGAKITVDSASLMNKG
                     LEVIEAKWLFGVDISRIDVLVHPQSVVHSAIELIDGTVLAQLGIADMRIPIAYALSYP
                     DRLTNPLPALDLAKTGNVTFCLPDTKKFPCLRLAYEAARAGGTAPVVLNAANEAAVAA
                     FLENKICFNDLPKVIEKILDRHSVKQNPSLEEILHADGEARWEAAGILKKLRRG"
     gene            complement(17895..18710)
                     /locus_tag="PLA58_01555"
     CDS             complement(17895..18710)
                     /locus_tag="PLA58_01555"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOR61511.1"
                     /translation="MATQNSNLQRWLTGLVLAAVLLLIIVMGSPVVLTAVITLVIVIG
                     LWEYNGIVFGPGHRAEKTESMIIAVLIPWIVLAGSTQWIAAVLTFAILMVFMVFLWQA
                     EGPSLDLSSVMKALFGLLYIPLLTSHFVLLRLLESGVFWVLLVLVIGIIGDTVALYVG
                     KSLGKRKLLEAVSPGKTVEGTLGLIAGATVAACVFGYFWLPQIHWAHFLILGFVGGII
                     GQLGDLCESAIKRSHGKKDASALLPGHGGLLDRMDSLIFLAPFVYYYRIFVIG"
     gene            complement(18730..19470)
                     /locus_tag="PLA58_01560"
     CDS             complement(18730..19470)
                     /locus_tag="PLA58_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOR61512.1"
                     /translation="MQKIDPDNLPGHIAIIMDGNGRWAKQHAMGRIRGHQKGAQAVRT
                     AVTACRELGIACLTLFAFSSENWGRPGDEVNALMALLADYLDKEAPKLQKREIRLTTI
                     GDIDRLYAPVRKKLLDVKKLTQSNDKMILNLALSYGSRDEIIAAVRKMLADSREGKLN
                     PDAVDSDMFGRYLYTHGLPDPDLLIRTSGEYRISNFLLWQMAYTELYFTDVLWPDFKK
                     DDLLKAIAAYQGRERRFGLTSDQISGRK"
     gene            complement(19658..20215)
                     /gene="frr"
                     /locus_tag="PLA58_01565"
     CDS             complement(19658..20215)
                     /gene="frr"
                     /locus_tag="PLA58_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOR61513.1"
                     /translation="MKEQIFSKFKDNMEKTISSLEKSFSRVRTGRASVSLLDGIKVDY
                     YGTPTPIAQVANLSVPESRLIVIAPWDVTTIGAIEKAIQKSDLGLMPSTDGKVIRLSI
                     PQLTEERRKDLVKIVKKMAEEGKIKLRNERRDANEALKELKKNNKMSEDEMHGAQDEV
                     QKLTDRYIEKTDKILAAKEKEIMEI"
     gene            complement(20218..20943)
                     /gene="pyrH"
                     /locus_tag="PLA58_01570"
     CDS             complement(20218..20943)
                     /gene="pyrH"
                     /locus_tag="PLA58_01570"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOR61514.1"
                     /translation="MGEKKKAAYKRILLKLSGEALLGRQSTGVDPDVVNYIADEIRSL
                     ADLKIQIGIVIGGGNIFRGVQAGARGMERTTADYMGMLATVINSLALQSALEAKGLAT
                     RVQSSIEMREIAEPFIQRKALRHLEKGRIVIFAGGTGNPYFSTDTAASLRAMEIRADV
                     ILKATKVDGVYSADPVLDHSAVLFKKISYIDVLTKNLKVMDSTAISLCRDNRLPLVVF
                     NLQKKGNIRGVVCGKKIGTYVGE"
     gene            complement(20943..21548)
                     /gene="tsf"
                     /locus_tag="PLA58_01575"
     CDS             complement(20943..21548)
                     /gene="tsf"
                     /locus_tag="PLA58_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOR61515.1"
                     /translation="MEITSTMVKELRTKTGAGMMDCKEALAAVEGDFEKAIDFLRKKG
                     LSAATKRSSKAAKDGTIASYIHMGGRIGVMVEINCETDFVAKTADFQTMARDIAMHIA
                     ASNPLYVRPDEIAEDALNREKEIYRSQLREEKKPEKIWDKIIEGKLKKYYEDVCLVEQ
                     KFVKNQDITVGTLISNMIAKTGENIVIRRFARFQLGEELKK"
     gene            complement(21601..22422)
                     /gene="rpsB"
                     /locus_tag="PLA58_01580"
     CDS             complement(21601..22422)
                     /gene="rpsB"
                     /locus_tag="PLA58_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOR61516.1"
                     /translation="MSAISMKLLLEAGVHFGHQTNKWNPKMKPYIFGARNNIYIIDLQ
                     QTVGMFQSAYNFVVDTVSQGREILFVGTKKQSQEAIREEATRCGMPYVNQRWLGGMLT
                     NFITIKKSIDRLNSLNKMFADDSVKAFPKKEITKLQKEMDKMETVLGGIKNMRTLPAA
                     VFIVDPNRERITVQEAKKLKIPLVAIVDTNCDPTDIDYIIPGNDDAIRAIKLFSSKFA
                     DAVMEGKKRFEEKLAAESNKEGGAPAAVQQDAEADIPESVEMENGSAAPAPEKHS"
     gene            complement(22605..23804)
                     /gene="argJ"
                     /locus_tag="PLA58_01585"
     CDS             complement(22605..23804)
                     /gene="argJ"
                     /locus_tag="PLA58_01585"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOR61517.1"
                     /translation="MTKSTNKVSVPGFLANGVSAGIKSGDRKDLGLIFSTIPAKVAAL
                     FTKNTFKAAPVLIDAERIKRGIAQAVLTNSGCANAATGKEGMADALTVSAAAAKQLKI
                     PEEHLLICSTGVIGKRLPVRKIEAGLGKLVRGLNEFGIEDAEAAMMTTDKFPKIAIRK
                     GIVGAKDITICGIAKGAGMIEPNMATLLTYVMTDALIDAKALNTVFHQVIDSTFNAIS
                     VDGCMSTNDTAIILANGLAGNNPLERAQARLQRFREMLTDVLAELAQAVVRDGEGATK
                     FITVVVEGAKSKSDARRVAYSVANSNLVKTAFFGEDPNWGRILSAIGASGILLEKEKV
                     SLSIGNMTVFAQDAPVNLAPARLKEIFQGGNIEVRISLGGGDKSYSVYTSDLSYDYVK
                     INADYTT"
     gene            complement(23826..26345)
                     /gene="secA"
                     /locus_tag="PLA58_01590"
     CDS             complement(23826..26345)
                     /gene="secA"
                     /locus_tag="PLA58_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOR61518.1"
                     /translation="MLNAILKKIVGTKNDRELKRLSILLHEVNQYEKELMALGDAQLK
                     EKTPYFKEKLKNGLTLDDILPEAFAVAREASRRTLMMRPFDVQVIGGIVLHEGKIAEM
                     KTGEGKTLAATMPLYLNALEGKGCHVVTVNDYLARRDAEWMSPIYSFLGLTVGVIVHG
                     MDDDERRKAYGADITYGTNNEFGFDYLRDNMKFSLEEYVQREFNYAIVDEVDSILIDE
                     ARTPLIISGASEESTDKYYKINQIIPRLRKDADYTIEEKSKTVVLTEEGVSHVEKLLN
                     VQNLYEPRNIEIVHHVNQALRAHTLFKRDVDYLVKDGQVIIVDEFTGRVMPGRRYSDG
                     LHQALEAKEKVKIEKENQTLASITFQNYFRMYKKLAGMTGTADTEAAEFKKIYNLDVV
                     VIPTNMPMIRQDHNDLIYKTEQEKIKAVIEEVKALNKAKRPVLIGTISIEKSEMLSKY
                     LTRAGVKHHVLNAKNHEKEAEIVAGAGQPGQVTISTNMAGRGTDIKLGENVADLGGLH
                     ILGTERHESRRIDNQLRGRSGRQGDNGSSRFYLSLEDDLLRVFGAERISSVMETVGLE
                     EGQPIEHKYISRAIENAQKRVEGQNFDIRKHLLDYDDVMNNQRKVIYEQRKKVLRGQD
                     LWVDVEAMVEEITGDLLSEFVGEKNHPQDWNLKALEDAVFQQFKLRPNIANAKDLTSV
                     DAIRSLIVENVRQYLRGKEKEFGPDLMTYLMKVIMLQAIDTHWKEHLLSMDHLREGIG
                     LRGYGQKDPVREYQREGYAMFMEMIGRIKMDTLEKLCLVRIQREEEVEEIRQKQKQDY
                     ILSRGEDTPAHQTVKRETEKIGRNDPCPCGSGKKYKKCCGA"
     gene            complement(26507..27412)
                     /locus_tag="PLA58_01595"
     CDS             complement(26507..27412)
                     /locus_tag="PLA58_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOR61519.1"
                     /translation="MSDKFFTLMIIPRRKSSVTKISLSGRLVYGLCAGTILAVLLALY
                     VVYDYAGIKRDRMELSRLRQQTAQQTKEINELALKIDGFADRMEEIKQFDRKLRILAS
                     EQVGPDGKTPLGIGGSNNDQIRLQQLIDEDRDKLVSGMHESIDRLNAEANERESSFNE
                     LLKFLREQKSLLAATPSLWPVRGWVTSGFGMRKNPFGSDREFHKGVDIASRLGKEIIA
                     PADGFVLEAAYRSDDGNIVRIEHGYGVSTSYAHLSRAAVKEGVRVKRGDVIGYVGDTG
                     RSTGAHLHYGVIVNNIPVNPRKYLK"
     gene            complement(27577..28434)
                     /locus_tag="PLA58_01600"
     CDS             complement(27577..28434)
                     /locus_tag="PLA58_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOR61520.1"
                     /translation="MGRLGLKIDVDTHTGMKNGVPRLLRVLRDFSVRGTFFLSIGPDN
                     SGRAVLQLLKNPLFLKKMLKTRAPSLYGWKTALYGTILPAPMIALSFPLLVRQILDEG
                     HEVQFHAWDHRRWQDDLPKKSEWWIEEWFEKGVSGFEKLTGRKPTAFGAPSWLIDDRV
                     LRIVGKYPFEYLSCTRAKAPFVHDSLRLPEIPSDLPCFEELAPQNDLAAIAVLMNDGG
                     DHILPVHAEVEGGVFEEKFRQLLKGALTAGMEIATLDDLKNSLNTELLPRRKYQMDLL
                     PGRHSPCAV"
     gene            complement(28449..29483)
                     /locus_tag="PLA58_01605"
     CDS             complement(28449..29483)
                     /locus_tag="PLA58_01605"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HOR61521.1"
                     /translation="MKILILGVNGFIGHHLVNRILAEKPDWQVFGMDLGTERIGAALH
                     NPRFNFLEGDISINREWIEYHIKKCDVIMPLVAIATPKAYVEDPIGVYHLDFEMNINI
                     VKQCVKYHKRVVFPSTSEVYGACADPEFDEDESYLSVGPIRKERWIYSCCKQLLDRVI
                     YAYGTRGSLEFTLFRPFNWIGPRLDSLDTAKEGSSRVVTQFIAELLLKRPINLVDGGR
                     QKRCFTYVDDGIAALMKILENKDDICRNQIINIGNPENECSVKELAALLKKLFQEHPD
                     HRQDSVYSDIIEIPADVYYGKGYQDIYTRKPRIEKAGKLLGWEPKIGLQESLRLTLNS
                     FLEENQAAIL"
     gene            complement(29480..30397)
                     /locus_tag="PLA58_01610"
     CDS             complement(29480..30397)
                     /locus_tag="PLA58_01610"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HOR61522.1"
                     /translation="MKAIVLAYHNMGLAGLAALRRHGFDIAAVFTHQDDPQENCWFGS
                     VKDWAENQGIDCATDAVNSPQTIEKIKRLQPDVLFSFYYRNMVRPEVLAIPRIGAFNL
                     HGSLLPSYRGRCPVNWVLVNGEEETGVTLHDMVEKPDAGDIVGQKAVKIDFEDTARTL
                     YDKLCKAAGGLLDELLPEMKKGKIPRIKQDLSRGSYYGGRKPEDGRIFWSESALHIYN
                     LIRAVTEPYPGAYAFAESGEKIMVWRARPDSSKAGGGPGDVILDGQSVLVKTGDGAIR
                     LLEIEASGARWKDAGIRDYFQQGKVKKLT"
     gene            complement(30394..31344)
                     /locus_tag="PLA58_01615"
     CDS             complement(30394..31344)
                     /locus_tag="PLA58_01615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR61523.1"
                     /translation="MPEMRISVVIPVYNEEANLKALMGRLLPVMESLAKPFEIILVDD
                     GSRDRSLALLKEFTSHPSIRVVELTRNYGQHAAIMAGFSILRGDMVLTMDADLQNPPE
                     EIPNLVRVMEEGGYDVVGTIRKGRKDSFLRILPSKMINVVARKITGVSMRDWGCMLRA
                     YSRPVVERMLACHEQATFIPALATVFAKRVTEIEVAHEERFGGKSNYPLRKLINLQFD
                     LVASFSDFPIKLIMYGGILMSLLGVCFGAFLAVARLVYGVRWAAEGIFSLFAILFVFV
                     GLQFFALGVIGEYIGRIFREVRKRPEFVIERIYGLHEDRP"
     gene            complement(31337..32488)
                     /locus_tag="PLA58_01620"
     CDS             complement(31337..32488)
                     /locus_tag="PLA58_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR61524.1"
                     /translation="MKIRKDFLPFSRPSIGETEINAVAACLKSGWITTGALCKAFEEQ
                     LCALTGAPRAVSLSSATAGMHLMLTALNVGPGDEVITPSMTFASTVNLIALRQAKPVF
                     VDVDYGTLNMDCEAMEEKITERTKVMIPVHFAGAPADMDRVNRLARKYSLKVIEDAAH
                     AVGTSYKGTHAGGFGHPAIFSFHPIKNITTGEGGMITLNSAAVEKKLRLTRFHGIERD
                     AWKRYGKGGNPSYDIAEPGYKYNMPDLLAALGLAQMERWRELNARRRVLAGLYFDGLR
                     GTEGLDLPEVPSYDHVHAWHLFVVKVTGMSRDLFMQKLADHNIGYGLHFPPAHKLSYV
                     KKRFGVRAASLPQTNLAADRIISLPLFPDMTENDVHYVCGAIKEILKNA"
     gene            complement(32495..34174)
                     /locus_tag="PLA58_01625"
     CDS             complement(32495..34174)
                     /locus_tag="PLA58_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOR61525.1"
                     /translation="MKKKAVLWIVPFLLYVALLGVMPQMEPDEVRYSLIASAMNETGS
                     YVTPKIKNTVYLEKPPLVSWVTAAAFKIFGENDFSARLFSGLCAWGCILLVFWIGRRF
                     RDEKTGLYAAAVLTISAFPFALGRINILDMPLTLFLCLAIWLGYLSVTEEKKRLLSYG
                     FYFACALAFLTKGMIGVVFPFAVLGIWLVWERRWRLLPRLISPAGIIIFLAVVCPWLY
                     LAQKENPDFLWFFFVREHFLRFTTQMHGKTEPFYYFLPVLIGGTLPWSIFLIRAWQPG
                     KWKEQLFRKEENRFLAVWFLFIFLFYTVSSSKLVTYIAPVFLPAALFAGCIFRGYDEA
                     PAQPTGRRKILYSLLAWGQFLIVGAALLLSPIVKSHHDPSRGLVIMISDLWWIYLLPV
                     FLAAGLMIFLPDWIARRKGAGWFPSLYLLCCIFLAGFLFPLNDFLYPYRSALVAKEAI
                     ARYVPQGKLLYQYRVNFYGIDFYNKIRTPIVEDFGELSEGIAKLPEAERKKYFLSRQD
                     FFAKCRQEKEVYCITQHEKRLKDLREQFAGMDMLWENGAFYLLRIRNQPKD"
     gene            34789..35946
                     /gene="lptF"
                     /locus_tag="PLA58_01630"
     CDS             34789..35946
                     /gene="lptF"
                     /locus_tag="PLA58_01630"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HOR61526.1"
                     /translation="MNKIINRYIFREIALPFIIILFVLTFVLLMGKILQIMDLMVNKG
                     IRVLDIGHLIVLIMPNFMLFTIPIALLVSILIAMGRLSADNEITVLKASGVSLLQLSY
                     PVAVASLMAFACAMVIGYFLVPQSNFASKRLLFELASQNAGIGIKEKVFNADFKGLLL
                     YADTVPADGESMENVIISDSRVLGEQNTILAKKAFLVADPKLMILKLRLEDGSIHTVG
                     KDLKNYRKVDFKSYDINLDLSSTFASFTDESKSSTEMTLSELLERMKKPALADAVVRE
                     LAIEVHKKFSIPLSCLFFALLAIPLGITHHRAVKSRGFAVGLIIVAAYYLLRIGGEAL
                     VENGRMDPALGVWAPNVLFALLGVFLFYLAHREISVSQTLIAIFQGEKPRG"
     gene            35951..37030
                     /gene="lptG"
                     /locus_tag="PLA58_01635"
     CDS             35951..37030
                     /gene="lptG"
                     /locus_tag="PLA58_01635"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOR61527.1"
                     /translation="MKLIDQYILKEFLRFFLITFVSFIMLFLIIDFFEKSRMFMSNHA
                     TVAQIASYILYSIPFVISMTLPVAVLLATLLTYSFLSKHSEITAMKANGISLYRISLP
                     VLAVAAAVSVFLFFFTEMITPASVQKMEHIIKVEVQKQQPLGFFKQNEIWYRSQNAIY
                     NFKMFDVEKNILHGVTINRLNPDFTMKTRIDAERAEWKNQSWVFYNLLVTTFQPNGPP
                     SLEWSKEKVVALPEGPNDFKIIQKDAEKMGYFELRRYVKKIRAEGYDVSRYLVDLQGK
                     IAFPLVCLIVVLIGMSFSVRSERSGGIMQSLALGLFLGFSYWIVHAFSMSLGRSGTLP
                     VFLAAWTANILFGGLGAVLFYRIRT"
BASE COUNT         8159 a        10542 c         9574 g         8758 t
ORIGIN      
        1 gtctccatcg ccacgccccg ttcgatgatg ttttccagtt cgcggatatt tccgggaaac
       61 ggatattcca tcaacagctc catggcgtag gaagaaatcg tccgcacttc cttgccgaat
      121 tcgcgcgcgt atttttcgat gaaatgccgg gtcaaaagcg gaatatcttc ttttcgctcc
      181 cgaagcggcg gcatgtgaat cggaatgaca ttgaggcgaa aatacaaatc ttcgcggaaa
      241 gcccccctgg cgactttttc cttcaggttc tgattggtgg cggagatgat gcgcacgtcc
      301 accgtaatat ccttttctcc cccgatgcgg cgaaaggtct tctgctgcac cactctcaaa
      361 agcttcacct gcaggacggg cggaagctct ccgatctcat cgagaaaaac cgttccgccc
      421 cgggccagtt caaacaggcc cgcccggtcg ccgtaggcgc cggtaaagga acccttcata
      481 tagccgaaca gctcgctttc cagcaggttt tccggaattc cgccgctgtt gatggccatg
      541 aacggcatcg aggcgcggga cgaattttcg tgaatggcgc gggcgaccag ctccttgccc
      601 gtcccgcttt cccccagaat cagaatattg gccggggtat cggccacctt tcgaatggtc
      661 gcgaagacct tggccatctc ccggctggtc ccgatcatgc cgcaaaacga agtctgggcc
      721 gccggatttt ctttcaacgt ctgtccctcg ctcagcgatc cctgctttaa cagggcctgt
      781 cggacaacct gcttcaattc gtcgatgttg ccgctttttt ccacgtaatc atacgcgcct
      841 tctttcatgg cattgacggc cgtctcgccg gaagcatagg cggtaatcag gatgaccacg
      901 gtttccggcc ggtgttcctt gatggttttc aaaaattcga tgccgtcgat tttgggcatt
      961 ttcagatccg taatcaccag atcataagcg gttttgcggc acaggctgat cgccttctcg
     1021 ggtttgcccg tacaggtgac gtcataccct tctttggcca gcatgatttc catgaactct
     1081 ctcatcccct gatcatcatc cagaaccagt attttcgcca tgcttgcgct cctttgatct
     1141 taccggcagg gcagccaaac cgtgatcgtc gtcccctcat ggacggcgct ctgtatcgcg
     1201 atcctgccgc ggtagccgtc cacaatgcgg ttgacaatcg ccagacccag ccccgttccc
     1261 cgctccttgt tggtaaagaa cggttcaaaa atcttttttt ggtccttttc ttcaatgccg
     1321 catccgtcgt ccgtgacttt gatttcggca tactcttttt catcttccag ccgcacggtc
     1381 cggatttcca gaccgatttg acccttcccg tccatggact gaagggcgtt ggtcagcagg
     1441 ttttgaagga tctgacggat ttgctcttca ttggccagcg cccgattttc cccggaaatc
     1501 tttttttcca gccggatgtc ttcggtccag tcggcggaca tttttacgct ttccaccact
     1561 tcttcggcaa cgtcattcag ccggatggac tgcctggctt cgggcacggg acgggccagc
     1621 agaagaaaat ccctcacgaa gctttccagg cggtctttgc cccgcagaat gatctgcatg
     1681 agtctcttgt ccgtttcatc cagatcaggg gtctgcgcca ttaactggat cgagccggta
     1741 atcgacgcca ggggatttct catttcgtgg gcaaggccgg cggccatttc gccgatcaac
     1801 gcaagcctcc tgcttttctc gagattttcc tccatgcgcc ggatttccgt catatcctga
     1861 aaaatcagaa tgtgtccgat ctgctcgttt ttgcggtctt tcaaaggaga aagcgcgtat
     1921 cccagataca tcggcgtccc ttccctgccg ttgattctta tttcaccctt ggatttcatc
     1981 agcggttccc ccccggcgga aaacaaaggg gcaaaaacgg gaaaaacgtc ctcgatctga
     2041 cggttttcca cggatcgaag cggacggccg gtaatttttt cggcggatcg gttgaacgtc
     2101 ttaatccggc cctgaagggt tgtcgtcatg atgccggtgt ccaccgattc gatgatgctg
     2161 cgaaaaagca gatccagctg atcaaacgcc gactcccttt ccgccagcag tgacctggcc
     2221 tttttttcct gctcgaccac aaagctggcc agaaaagcga ggatgtaaaa cgacaggata
     2281 tggacggcga tccggatgaa aacatccccc gcctccagtg tgtaatcgcg gccggtgtcg
     2341 taaaaagtgg ggattacgcc gaaatattcg aggtccagca ccagcccgta cgcaatactg
     2401 caggccgacg caaaaattaa agcgcccctg cgccccagaa aaatggcggc atagatgatg
     2461 accagcgtgt aaagcaggga gtagttgctg ccgatgctgc cggtcagata caccagaaaa
     2521 gaaaccagcg cgatatccac ggccagctga aaatagatat tgatctgaaa atactttttt
     2581 tttctcaaaa gcagtgtata aatgacggac aggatgaaca tcgcgacgat cacggcataa
     2641 atggcgtgca gaacatcccg ggagatgaaa atgatcggtt tgacggcgcc aagaaacagg
     2701 gttaccagca gaaatgcgga gatgataacc accctgctca cgatcagcag tttgagcctt
     2761 cgccaggttc tctcgtcata ttccatcggt tgccgcatag actgtgccgt tgctcttccg
     2821 cgtccgtatt ttgaatcccg cgtaagcggg acgagcgttc attctccgca gcttgctgcg
     2881 atagtatgac gttcatttca tacctcgaca gcttgctgcg aggtggttga tctgctttta
     2941 taatatttta tattttttag ccaatcaatt gagcggttcc cgctgagagg acctgtcccc
     3001 catgcgcaga cccttatggt atcctctgat cgccctgatg accggtattc tgatcggtga
     3061 tcgctttgtc ctggccccgg aagttttgct ggccggaatg ctgccggttc tggcgctcct
     3121 gcttttgtgc ctcaggcgcc gttggaatgc cgccgcgctt attgcgcttc tcctgttgac
     3181 gctgacggcc ggaatgctca atattcaaag gcaacccttc ctcgcccgtc aggagaagca
     3241 tgttctgcac ctggccgacc agggaaagat gactctggag ggcgttgttc tttccaccga
     3301 acaggcgttc cctggaaaaa gcacgctgat cgttctttgc cggcgagtcc ttcaaaacca
     3361 gacgcccgca cccgtaacgg ggaaaattcg cgtggcgata ccgtccgacc tttcgtttca
     3421 gtacggagat ttcatccgtt ttcacacgaa aatcaagaaa attcaaggct ttcacaatcc
     3481 gggaagcttc gattacgagc gcagccagaa ccgccgcggc ctttatgttt ccggatttgt
     3541 ctccgaccgg gcaggcattg ttctgatccg ccctgacacc gcgagcggcc ttaaactgaa
     3601 gctggaacgc ttccgtttgt atctgaaacg cctgctggaa gtccacgccg cctccccgca
     3661 gagagaaatc ctgcaggcca tgaccatcgg cgatcaaaaa gccattccgc aggacgtgcg
     3721 cgaccaattt gccaaaaccg gcacctcgca tattctgtcc atttccgggc tgcacgtcgg
     3781 catcgtcgcg gcatcggctt ttttcctgat gcttctggcg ttcaaatcct cggaatattg
     3841 catgctgaaa ttcaacatca tcaagaccgc gacggcggca gccatcgttc cggttctgat
     3901 ctatgcgctg gtcgccggga tgggaacccc cgttctccgt tccacgctca tgacgctggc
     3961 tttccttgcc gcgctgctga tcggcaggcc gaaagacctc tacaatattc ttgccggcgc
     4021 ggcgctgatc attctgatcg cttcgcccga agcgctgttc gagatttcgt tccaactgtc
     4081 tttcagcgcg gtcctggcca ttctttacgg ggttgaaaag ctgggcaaac ggcaaattcc
     4141 cctgccctca gcagcgcccc gctggattgc ggcgggcgtc aaccgcctgt acctttttct
     4201 tctggtctcc gccgcggcta cgctgggaac cctgccgatc atcgcctttt atttcaaccg
     4261 cgtttcggct gtggccctga tcgccaatct ggtcgcggtc cccctgctgg gcatcctggc
     4321 gctgattccc gccatgatgt tcatcctgac gtctgtgttc tcggctgcgc tggccggttt
     4381 tttcatttcc atcgcttctt ttttcaccgg gattgccgtc agcatcattg aggagctggc
     4441 tttgcttccc tggtcatctt tgagttttat aaagccaaat gcccttgaaa tagcgctttt
     4501 ctacgcgttc atgtacttcc tgatcgaagc cctgacaacg gacagaccaa aagaccttca
     4561 ggacgttccg gcgcgacggc cgctgtggat caaaaccggc cttctggtct gcgccgcgct
     4621 gattcttgcc gatgccgcct tccttgttct gaaagaccag tattccagcc atttgaaaat
     4681 cacggccatc gatgtcggcc agggctcgtc tgctcttgtc cggatgcccg ggggcgtaaa
     4741 catgctgatt gacggcggag ggtttcacga cagttccttt gacatgggcc gggccgtgat
     4801 cgcccccttt ctttacgcca aaagaatacg aaaggtcgat atcgccgtgc tgacacatcc
     4861 tcaccccgat caccttcagg ggctgattca tatcgtcaat gcctttgacg tccgggaagt
     4921 atggtgcgcc ggtctcacag cggacaatga tctctaccgg ctgtgggaaa aaacggttgc
     4981 cgaacgcggc gtgaaggtca gaaccctgtc ggcgcagtcg ccgcctgagg ttgtatccgg
     5041 cgtgaccttc cagtttctgt ggcccttgcg tccgccgcct cctggcgttc aggggatgtc
     5101 ctaccaggag accaatgacg cttccctggt catcaaaatg acctacggcg ccaggcgatt
     5161 tctggtgacg ggcgatatct ccgcggccat tgaagcgttc ttgaccgctt cggggaaaga
     5221 cctcaaaagc gatgtccttt ttgtgccgca tcatggctcc ctcagttcca gttcgccgga
     5281 ctttctccgt gtcgtatcct gccgctatgc cgtcatcagc gccggaaaaa acaatgtgtt
     5341 tcgccacccc catcccgccg ttctggagcg ctaccggtcc gccggcgccg ccatcttccg
     5401 cacggaccgg gacggcgccg tgtccatcga ttctgacgga cagtctcttt ccgtcactcc
     5461 ctggatcaaa aaaccggacc cggacttcgt tagacgccgg gccccgaata tttcattgcc
     5521 aacaacgggc tgatatgcta gaagcactcg atttattttg agatatgact atggaaaaaa
     5581 tcaccgccat caaaggcatc aaggacattc ttcccaagga ttcacccgtc tggcaccggg
     5641 tggagctgac ggcccgcaga atttttgaat gcttcggttt ttgcgaaatc cgcgtcccca
     5701 ttatggaaaa aacgtcgttg tttcagcgcg gtatcggtga aaccaccgac atcgtcgaaa
     5761 aggagatgta cacgtttcgc gaccgtgatg acgaactgtt gacgctgcgc ccggaagcca
     5821 cggcatccgt catccgcgcc tacattgagc ataacctctc cgcgtccgat ccggtgacgc
     5881 ggctttacac catgggcccc atgttccgcc gcgaacgccc gcagaagggc cgcttccgcc
     5941 aatttcacca gatcgacgtg gagcttttcg gggacgagag ccccgcttcc gacgcggaag
     6001 tcatcttcat gctgatgcac ttcctgcagt ctctgaacat tgaaaacctg tctctggaga
     6061 tcaattccct gggctgcaaa gcctgccgtc cggttttttc ccgggcggtg gtagattact
     6121 tgaaaggcca ggaaagttcc ctgtgccccg actgccggag acgcatggac gtaaacccgc
     6181 tgcgcgtctt cgactgcaag gtggaatcct gcggttcggt gattgccggc gccccccgca
     6241 ttttggattt cctgtgtgcg gattgtctgg agcatttttc ccgggtcaaa agctatctta
     6301 atgaactgaa gctgacgtat gaaatcaatc cgcggatggt gcgcggcctg gattactata
     6361 cccggacggc ctttgaagtg aaatccggcg agctgggcgc gcagaattcc ctggcgggcg
     6421 gcggccgcta tgacggactg gtccgttttc tgggcggccc cgacgttccc ggcatcgggt
     6481 tcggcgtcgg aatggaaaga ttgattgcct gtcttccgcc tgccggtgaa gtttcctctt
     6541 cggcggatat ctttatcgcc gcgttgggag agcgcgcgca gaaaatcgcc tttacgctga
     6601 cccaggaact gcgccggaga ggaatttgcg cggccatgga ttattccggc aaaagcctca
     6661 aaagccagat gaagcgcgcg gataagctca acagcgccta cacgctgatc ttcggggaca
     6721 gggaaatcga ggagaactgt gtcgaactgc gcgacatgaa ctcgaaaaat caacaacgtc
     6781 tgccgctgga ccatcttctg gaggcggtta cggatctttt aaaagcgagg taaatgtttt
     6841 gtgagtcggt ttattacaat agaaaaaaga acccattact gcggaagttt aaccggcgcc
     6901 gatgaaggca ggcaagtcgt tttgatggga tgggtgcacc ggcggcgcga tcacggcggc
     6961 gtcatttttg tcgatttccg ggatcgcgag ggcatcgttc agattgtttt taatcccgaa
     7021 gcgggggaat ccgtccatgg agaagcgcac aaaatccgca gcgagtacgt tctggccgtg
     7081 aaaggcagag tgtgcagaag gcctgcgggc atggagaatc cggcgctttc caccgggcag
     7141 gtggaagtcg tcatttccga actggaaatc ctcaacgaat ccaaaacacc gcccttctcg
     7201 tttgacgatg aagacatctc tgaaaatgtc cggctcaaat accgctatct ggacttgagg
     7261 cggccggcca ttcaaaaaaa ccttttcctg cgcagcaggc tggccattgc cacgcggcag
     7321 tatttcaatg acaacggatt tattgaagtg gaaacgccct tcctgaccaa gagcacaccg
     7381 gaaggcgcgc gggattatct cgttcccagc cgcatctcca agggcatgtt ttacgcgctt
     7441 ccccaatccc cgcagatttt caagcagctc ctgatggttt ccggatttga ccgttatttc
     7501 cagattgtca aatgcttccg cgatgaggat ttgcgcgccg accgccagcc ggaattcacg
     7561 cagatcgacg tggaaatgtc cttcgtctcg gaagaagaca ttctccggat gatggagggc
     7621 ctgatggcgg cgattttcaa ggcctgccgg aacatcgatc tgcctcttcc gtttccccgg
     7681 ctgacgtacg ccgaagccgt cagccgctac ggcaaagaca accccgatgt gcgcttcggc
     7741 atggagatca cggacctcac ggccatcgtg aaggattcgg gcttcaagct gttcgccgaa
     7801 gtcgccgctt ccggaggcgt cgtcaaagcc gtcaaagccg accaggcttc ttctctttcc
     7861 cggaaagatc tcgacgctct gtctgatttt gtcgccatat atggcgccaa aggactggcc
     7921 tgggccaagg tcaacgcggc cgactggacc tcccctatct tcaagttttt gaagccggaa
     7981 gaaacggccg ccatcggccg ggccatggat gccaaagaag gcgacatcct cttctttgtc
     8041 gcggacacgc ccaaaacagc ctgcgacgcg ctgggcaatc tgcgtctgca cctgggcaaa
     8101 aagctgaacc ggatcgaccc ggacgcgctg gccttcacct ggatcaccga gtttccgctg
     8161 atggagtatt cggagtcgga aaagaggttc gtctccacgc atcatccctt cacctcgccg
     8221 tttctggagg acatcccgct catgaccacc gatccgggca aagtgcgggc cagggcctat
     8281 gacctggtgc tcaacggctc ggagatcggc ggcggcagca tccggatcca ccgcaaggac
     8341 atccaggcgc aggtcttcag cgcgctgggc ctgagcgatg aggaggcaag gctgaaattc
     8401 ggttttctgc tcgacgctct cgaatacggg gcgccgccgc acggaggact ggcgttcggt
     8461 ctggatcggc ttgtgatgat catgacggga gcggaatcga tccgcgacgt catcgctttc
     8521 ccgaaaacac agaaggccgc ggacctgatg tccgacgccc cgtcgaaggt gagcattgaa
     8581 cagctgatgg aattgtcgct gaagatcgtt taattttccc ggcagtaccg gtcaatttcc
     8641 agggcgacct ttttggcaaa ggccacggcc tcgacgaccg ttttcggccc cgtgacgata
     8701 tcgcccgcgg caaacacgcc cggggatgag gtatggccgt tttcatcggc ggcgaccagc
     8761 ccgcggtgtg ttttggaaac gttgctgtcg gccatcaccg cgccctgcgg accctgaccg
     8821 atggcgacga taatcgaatc ggcgggaatc ttttgcgtgc gggaaaaatc ctcttcgtaa
     8881 accgttccgc tttcggtttt ttcagcgcga acggcaacac acttcacgcc gtcttcctcg
     8941 agcttcagcg cctgaagaag atggacaaat tgaatcccct cccggaccgc atcgtcgatt
     9001 tcctgccggt tgccgaagat ttcttcctcg agcagattgt tgatcaccgt cacggcgccg
     9061 cccggttcga tgctgcgaac cgcgattctc gcggcatcca gcgcgacgtt tcccgcgccc
     9121 acgacaacca cgttccggcc gaggcggtag gcctccgggc ttttgaggta gtcgatggcg
     9181 taaaaaacat tgcccagcgt ctcgcccagc agaccgagct tgttgggttt ggccgtgccg
     9241 accgcgatga agacggcctt atagccgtcg atgaacatat cgtcgagcgt gatggaggac
     9301 ccgataaaag tgttgggctt gaattgcacg cccagttggc gcaaaatggg atcgtagcgg
     9361 tccacgattt cccggggaag ccggaagccg ggaatgccgt agcgcaaaac accgccgatt
     9421 ctgtcgcggg cctccatgag cgtcacccgg tatcccatga gggacaggag aatgctcatc
     9481 gtgattccgg ccggaccgga tccgatcacg gcaatgcggg cgccgttctt ttccataacg
     9541 ggcggctcat aggttttcag aaaggtccag gacaggaact gctcgatttt gaaaaagtcc
     9601 accggctcgg attttttgcc gagcacacag tggccaaaac agtttcgctc atgcgggcag
     9661 acgatggagc acacggccga aagcggattg ttggcaaaca ggatttgggc cgcctcctgc
     9721 tcttttcctt cttccacgag cctcgtcacg tcgggaatcg gcgtggatac ggggcagtgc
     9781 ttcctgcact ggggaacctt gcatttcagg cagcgggcag ccatctgctg gatcaagtcg
     9841 aacgtacctc ccttttgtcg aatcttcata gtccaaagcg cccgcggttt caagaacgat
     9901 ttcagaaaat aatgattgcc gggagggaca aaggttgcta atgcactgaa aaacgcgaag
     9961 gatgaagaaa tccgaagttt cgaaaaaaac gaaagtccct tcggaaacgg aaggatcttg
    10021 atcgcaagga gactcttcat gaactcaaaa actttttctg aaaaagacaa acagatggcg
    10081 aaaaagtgcg ttgaatgtcc ggtatgcagg caggccagaa aacaccagaa aggcctggcc
    10141 ttctggtttg tcaaaaatat cgaacaagga atctgtccgt attgtgccgc gtatgaaagg
    10201 gtttacggga aaaaggccca cgaggcctag gcggcaaaag gcagggccgc cgcgttaaag
    10261 acgcttgtca aaagggagga gaaaatgaaa gatttcaagt atatgagcat ggctgaccag
    10321 gccatggaca aaatccgtaa aggcgctttt ctgaccgttg cggctgaagg ggagctcaac
    10381 accatgacca ttggctgggc catgttcggc gtggtctggc agaaacaggt tctgatggtg
    10441 gcggtgcgcc gtacccgtca caccttcggc ctcatggaaa aggcttcaga cttcacggtt
    10501 accgttcctt ccggcgatat gagccgggaa acggcatttt gcggaacaaa atcgggccgg
    10561 gatgtggaca agttcaggat gtgcaacctg aaaacggcaa atggacggca cgtcgcatcc
    10621 ccgatcattc agactccggg aattcatttc gagtgcagaa cggtttataa atccgccttg
    10681 aatcctgcaa atctggattc gggcctggac caaaccatct atcccgggaa agactatcac
    10741 acgctgtact tcggcgaggt tctggcctgc tacgaaacgg aatgacagga gaagctatgt
    10801 ccggaaaacg aaacgacctc ctgcccggaa atgcgacgga agcgcagggg cttttgatca
    10861 aggcttttca cgaaaaatgc ggcgatgaaa ttctgcctgt catcgacgat attctcggcc
    10921 ggcagggacg cgcgctgggg ctgaaagtaa aaaacaagtt gcccgattgc cgtctgtccg
    10981 ctgttgccgc cgctttcacg aagaactttg acccgtcgac cgtccgggtc gtttccctgt
    11041 cggacgaaaa atttcatatt cagggaagcc actgtccatt cggtctggag aacacatcgc
    11101 ggcagctctg cgaggccgtc atggcgattg accgcgaata tttcttcacg gcaaccgacg
    11161 gccaaaccac gctggagatt ttaaaaacaa gggcggcggg agatccgatc tgtgacacga
    11221 tttatcgcgc tgcagcctat gatgcggaca aggcctgacg cgccctgaca caccccactt
    11281 tagacatcgt caaaaagcgc ccactgttca aaggggcgct gctcgtcctg cggcgacaga
    11341 ggggatattt catctacaaa acgggatgag gaaagcgtca gcgtgcccgc ggaacggctg
    11401 taatccagca ctggatagga aaggtaaagc tgatctttgg cgcgtgtcac cgccacataa
    11461 aacagcctcc tctcctcttc ttctccgccc ggctccctga gcgcgcgctg cagggggatc
    11521 atgccgtcgg cgcacccgat gataaataca taagaccatt ccagtccttt ggcctgatga
    11581 atcgtgctca aaatgacccg gtcggcctgc cgctcctcaa tgtcttcttc tttggacata
    11641 ttggttaaga gcgccagttc attgagaaac tcatccatcg tctcgaaccg ggtggagaaa
    11701 tttcccagct gcatcagatc ttcttcccgt ccggtcgcgt cggaataagt gtcctgaaga
    11761 tgcgtccggt aaccgccctg attcaggagc agatcgatga tttttcccgg cgtccggtcg
    11821 gcggcctcca gggaaagcaa taacagcaag gtcttccggc atttttcgag gcccgcctgc
    11881 gcgggtttgg gagccgtttt gagaaacgat tccgaaagca gcgcctccag aggattttcc
    11941 tgtcgggaaa gccgggccca gattttttca aacgtgatct tgccgatttt gggatacatc
    12001 atcatcagtc ttcgccaggc cagttcatcg tccggacgga ccagcacgcg catgtaagag
    12061 gtcacatcct tgacgtgggc ctgctcaaaa aaacggatgc cggagcggat gtcgaaggga
    12121 atgtcgcgcc gcaccatctc catctgcact tccatcgaat gatagtgcgc ccggtagagc
    12181 acggccatgt cgccgaaggc caccccggcg cgggccagtt cggcaatccg ctgcgcgaca
    12241 aagtccgcct gcctcaaaac attttgcgcg gaaacgagga tgggcctggt tcccctgccg
    12301 cgaaccgctt tgagcgcctt gggaaactgg ttttcgtttt ggtggatgct cagattggcc
    12361 agatgcagaa tttccggcgt gctgcggtag ttcgtctcca gtttgaagat tttacagtcg
    12421 ggatagcgct cgggaaaacg gagaatattt ttaaaatcgg cgccgcgaaa agaataaatg
    12481 ctctgcgaat catctcccac cgccatcagg ttgcggtggc cggaggctat caaatcaacc
    12541 atttcggcct gaagaacatt ggtgtcctga tattcgtcca ccagcacata ctgaaatcgt
    12601 tgggaaagcg tctgcagcac atcgggatgc tccctcagga gacggcggcc attggcgagg
    12661 aggtcgtcaa aatccatcac ctggagttct ttttttttct gcgcatagcg ccgggcaatc
    12721 gaatggattt ccgggctcaa atgcaaaaag aacgggtacc gcgattccag cacgtcctcc
    12781 agtgtacttt cggtgttgac ggcgaggctg atgatttcgg caaggatcgt gtttttggga
    12841 aatttggaga gactggcgtc gatgtggagg tcgtccatgg cctgccgaat gatctggcgc
    12901 gcatcctcgg tgtccacaat ggaaaaacgg tgggaatagt ccagacggga ggcgtagctt
    12961 ctcaacaggc gatgggcaat ggaatgaaac gtgccgccca gaatgtttcc cgcgccgcgg
    13021 cccaccagac tttccacgcg gcagagcatg gaacgggcgg ctttgttggt gaacgtggcc
    13081 agcagaatgc gctccggcgg caccccgtct tccagcaggc gcgccacccg gtacgtcagc
    13141 gtccgcgtct tgccgcttcc ggcgcccgcg agaacaagaa gcggtccgcc tttttccgtg
    13201 accacccgat actgctccgg attcagctct ctttcatacg caatcatttc aacccggggc
    13261 cttcaggcgc caagcgcttt tttaatttcg gcaaccgtat ctttggaaga aagagacaga
    13321 aatgttttca gcagcccctc tttttcataa aaaccgatga gcggcgctgt tttcgcattg
    13381 taggtctcca gacggttgga aatcgcttct tccgtttcat catcgcgctg aacagtctga
    13441 tgatcgcaca gcgtgcaggt tccgtcttcc cgcggtggat tgcttttgac gttgtaaatg
    13501 gcctggcagg ccggattgca acaggttctg cgcgtggtca gacgatccag aatcacgtcg
    13561 cgcggcacat ccagattggc gacaaaatca agcgcaatgt tcagtttggc cagcagcttt
    13621 ttgaggtcct gcgcctgagg aatcgtccgc ggaaaaccat ccagaatgaa ccccgtttga
    13681 caatccggct cctgcagcct cgcctccatg atgtccatga tcagcgaatc gggcgccaga
    13741 tccccccgat ccatatattc cttcgccttc ctgccgaggg ccgtcccctc tttcacggcg
    13801 gttcgcagaa tatctcccgt ggaaatctgc acggatccgt caaattccgt aagcagcttg
    13861 gcgactgtgc ctttgccggc gcccggcgcg cccagtaaaa taattctcat gttttcctcc
    13921 tgatacgaag cattcccatc ccgcttaagc ccgacgagcg tttttttctc cgcagcttgc
    13981 cgcgagggga tggattggaa caaactgcgg cgcttataat gcaaaaccgg cggacggtca
    14041 agtctttacg gcgacagttg aatttcaaaa aggtgaggcc agtatttgcc ggtcacaaac
    14101 atgcggtttc tcttcccgtc ataggcgatg ccgttgagca catccacgcg ggcattggcg
    14161 ggaagataag aacgaagcgg ggataaatca atccatcccg ttatcctgcc ggtgtggggc
    14221 gaaatgcggg caatgatgtc ctccagataa atattcgccc agatttcccc ccggacaaac
    14281 tccagctcat tgagcccgga aaccggcctg tccccgtcgc tgacatggat tcttcttcgg
    14341 attttaaagg attccgggtc gcggaaaacg atcgtcgaag aaccgtcgct catgagcagg
    14401 cttttcccgt cggaagccag cccccagccc tctccttcgt attgaattct ccggatttcc
    14461 ttcagggagg cggcatcata caccagggcg gtttggtttt gccaggtcag cagataaatc
    14521 cggtttttga aaagcgcgat gccctctccg aaaaactccg taggcagttg aatctgcctg
    14581 agaactttcc cggtcgcgat gtctttgcag gccagcgtcg atcgccccag caggccggtg
    14641 gactcgcaca aagaaccgtc catgatgatc agtccctgcg tgaaagcgtt tgcgtcatgc
    14701 ggaatgattt tttgaactgc gacggaggaa atgggcgcct ttgtttgcat caggatggaa
    14761 aacgggtttt tgtccggcag aacattttcc gcgcctgcct cgcccggccg taaaacacag
    14821 atcgaaatga aaatcagtgc aacaaagatt ttttcgatat tccgaatcat ggcggaccgc
    14881 ctgtgcggga gcaacagaat tcccttgatc ttccggggcg ccgcgacaac cggccgcgcc
    14941 gtcatcctcc gctcctcgcc ggcagggcat ccactgagcg caaataaacg ggcgcgcacc
    15001 gggccggatc cagcaaatcc ccgcgaaaat atttttccag cgcgagccga gcaacggctg
    15061 cggcgctgac gaattgctgc tcggccggcg ccattttatt tttcggcgaa gtcaggggag
    15121 gaatcatccc ggcataccgg acgacgccgt cgccgacata aatgaccggc tcggaaaaag
    15181 gatcctccac gctgcgcggg ctctgagcct gcggttcccc gaccggatgg aggatgccgt
    15241 cctttccgca ccggtagtag gccagataca cctgaccgcg gccggcatcc atcatcgaac
    15301 cgatcacggc gtccggctcc tccacgttga gcgccagagc cgcaagcgag gaaactcccg
    15361 ccgccggctt gcccgtcgcc agcatcaggc ctttgagcgt gctgacgccg atgcgcagcc
    15421 cggtaaagga gccgggcccc agggtgcagg cgaacaaatc catatcccgc actgtgacat
    15481 tggacaggcg gcaggcttcg tcaatggcgg ggagaagcac ttccgaatgg ttttgctccc
    15541 gatgcagaat gacatggtgt aaaaccgtcc ggttcctgag cagggcgacc gacagggttt
    15601 tggaggatgt gtcgaaagcc agggtcagca tattatttga aaaattttaa aacatcgttg
    15661 atgggtttga tattgagcct ttcgatgtcg atcatgatga caaaaagcat cagcatcaac
    15721 aaaacgacaa aaccgatttg ctgcgcccat tccttgattt tgaccggaat ttcccttcgg
    15781 gtcgccatct cgatgagata aaagaagata tggcccccgt ccaggacggg aatgggaaac
    15841 aaattgatga ctcccagatt gatggaaaga atcgccataa aaagaacaaa tggaataatg
    15901 ccttccttca cctgatcgcc cgccacctgg gcgatgaaga tgggtccgcc cagtgtccgg
    15961 ggcgaaagca ctccctccag cattttcacc acggaaatga cggtcagctt gctgatttcc
    16021 caggttttat ccaccgctga aacagcggcc gcgagcggat ttttcctttc gatgaccgtc
    16081 cggccggccg gcgaaatgcc gatcagatag gtcgattcct cttcgccgaa aatatttttt
    16141 gcccgggaga gtttcggttt gacggcaaag gttttctctt cagaaccgcg ttgaaccagg
    16201 atggagagat tgcggcctcc ggactccttg atgacgggcc ggatctcgtc ccagaatttt
    16261 atcgggcggc cattaatcga cagaattttg tcgtctttga ggatgcccgc ctggagggcg
    16321 gcggaatccg gcgacagctc tccgatgacc ggaagaaggt tgggcagacc gtacaggtaa
    16381 acgataaaga aaatgacgat ggccagaaga aagttgaaga cgggaccggc cagcacaatg
    16441 agcagccgct tccatctcgg ctgtttgtaa aaagaccgct ttgcatcttc cggcggcagc
    16501 tcttccgttc ccgattcgcc gagcagtttg acgaatccgc ccagtggaat ccaggaaaga
    16561 acatattccg tttcgccgat tttcttcccg aagattttgg gtccgaaccc cagggaaaat
    16621 ttcaggacgc cgacgccgcc tgcgcgcgcg gccagaaaat gtcccagttc atgcgcaaaa
    16681 atcagaatcc ccagcaacac gataaatgaa atgaaggtaa ccaatcgatc atcctctccg
    16741 cagctttttc agaatgccgg ccgcttccca tctggcctct ccgtccgcat gaagaatctc
    16801 ctccagcgac gggttttgtt tcaccgaatg ccgatccaaa attttttcta ttactttggg
    16861 aagatcatta aagcaaattt tattttccag aaacgcagcc accgccgctt cattggcagc
    16921 gttcagaacc accggggcgg tccctcccgc ccgcgcggct tcatacgcca ggcgaaggca
    16981 gggaaacttt tttgtgtccg gcaggcaaaa cgttacgttg ccggttttcg ccagatccag
    17041 cgcaggcaga ggattcgtca gacggtccgg ataggacaat gcatacgcaa tgggaatcct
    17101 catgtcggca atgcccaact gcgccaggac cgttccgtca ataagctcga ttgcggaatg
    17161 cacgacgctc tggggatgaa ccaaaacatc aatgcgggag atgtccacgc caaacagcca
    17221 tttcgcctcg atcacctcca ggcctttatt catcagtgac gcggaatcca ccgtgatttt
    17281 cgcgcccatt ttccagcgcg gatgccggag cgcctgcgcc ggcgtcactt ttttcaaatc
    17341 cgcccgcgag aaggaataaa acggaccgcc ggacgccgtg agaataatcc tttttacgtt
    17401 tttcggcttc tgtccggcaa ggcattgaaa gatcgcgctg tgctcgctgt ccacgggaag
    17461 aacgcggatt ttcttttgtt tggctttttt cgtcaccaga tgcccggcga tgaccatggt
    17521 ttccttgttc gccagcgcaa gatccttccc cgcttcgatc gccgccaggg tgggcttcag
    17581 gcccgccgcg ccggaaatgg cggaaagaac catgtcggcg ctccgatgcg ccgccacgtc
    17641 ctgcgcgccc gcttccccgc agagaatgcg caccgggttc cggcttccca gaaggtcacg
    17701 aagatgtttt gcatcctccg gtgtttgaac ggaaacggct tcggggtgaa acgccagcgt
    17761 ttgccgggcc aaaagcgcaa cattgcggcc cgcggcgaga gccgccacac gaaatttttt
    17821 cggatttttc cggatgacat ccagcgcgct tgcgccgata gagcctgtcg agcccagaat
    17881 tgttattttt ttcatcagcc aatcacaaat atccggtaat aatacacaaa aggcgccagg
    17941 aagatcaggc tgtccatccg gtccaacaat ccgccgtgtc ccggcagcag agcactggca
    18001 tcctttttcc cgtggcttct tttgatggcc gattcacaaa gatcacccag ttggccgatg
    18061 atgccgccca caaaaccgag gatcagaaaa tgggcccagt ggatttgggg cagccaaaag
    18121 taaccgaaga cgcaggcggc tacggtggcg ccggcgatga gccccagcgt gccctccacg
    18181 gttttgccgg ggctgaccgc ctccagcagc tttcttttgc ccagggactt gcccacgtac
    18241 agcgccaccg tgtctccgat aatgccgatc accagcacca gcagaaccca gaaaacgccg
    18301 ctctccaaga gccgcagtaa aacaaagtgc gaggtcaaaa gcggaatata cagaaggccg
    18361 aacagcgctt tcatcacgga ggacagatcc agggaaggcc cttcggcctg ccacagaaaa
    18421 accatgaaca ccatcagtat cgcaaaagtc agaaccgcgg cgatccactg ggtactgccc
    18481 gcgagcacaa tccaggggat caaaacggcg atgatcatgc tctcggtttt ttccgcacgg
    18541 tgccccgggc cgaaaacaat gccgttgtat tcccaaaggc cgatgacaat caccagggta
    18601 atgacggcgg tcaggacgac cggcgatccc atgacaatga tcagaagcag cacagcggcc
    18661 aaaacaaggc cggtcagcca acgctgaaga ttggagttct gggtcgccat cgctttctcc
    18721 tttggattgt cattttctgc cggaaatctg atcactggtt aagccgaaac gtctttcccg
    18781 gccctgatag gcggcaatcg cctttaataa atcgtctttt ttgaaatcag gccataaaac
    18841 atccgtaaag taaagctccg tataggccat ctgccagaga aggaaattgc tgatgcgata
    18901 ttcgccgctc gtccggatca acagatcggg gtcgggcagg ccatgcgtgt acaaatagcg
    18961 tccgaacatg tccgaatcca cggcatcggg attcagtttt ccttcccggc tgtccgccag
    19021 catctttctg acggccgcga taatctcgtc ccggctgccg tagctcaacg ccagattcag
    19081 aatcatcttg tcgttggact gcgtcaattt tttaacgtcc agcagctttt tccttacggg
    19141 cgcgtacagg cggtcaatgt cgccgatggt ggtcaggcga atttcccgct tctgcagctt
    19201 cggcgcttcc ttgtcgagat aatccgcaag cagggccatc agcgcattga cttcgtcgcc
    19261 gggcctgccc cagttctccg aagaaaaagc aaacagcgta aggcaggcga tccccagttc
    19321 ccggcacgcc gtaaccgccg tgcgaaccgc ctgggcgcct ttctgatgcc cccgaatccg
    19381 ccccatggca tgctgttttg cccaccgccc gttgccgtcc atgatgatgg cgatgtgtcc
    19441 gggaaggtta tcgggatcta ttttctgcat tgcaattccg gtctttcttc aggccagccc
    19501 gcctgcaaac gaccgcgacg gggcaggctg gaagcggttt ggacctccgc cggaaaagca
    19561 tggactgaac caaagggatc cgaaagttgc atccggcatc aaaaacaaat ggggagcttc
    19621 atactcccca tttgcgtctg ctcgattgcg gtccgcctta aatctccatg atttcttttt
    19681 ctttggccgc aagaatcttg tccgtctttt cgatgtaccg gtccgtcagc ttctgaacct
    19741 cgtcctgagc gccgtgcatt tcgtcctcgg acattttatt gttctttttc aattctttca
    19801 gcgcttcatt ggcgtcgcgc cgctcgttgc gcagcttgat tttcccttct tccgccattt
    19861 tcttcacaat tttgaccagg tcctttcgcc gctcctccgt caattgcgga atggaaaggc
    19921 gaatcacctt gccgtccgtg gaaggcatca atcccagatc ggatttctga atggccttct
    19981 cgatggcgcc gatcgtcgtg acgtcccagg gagcaatgac aatcaaacgg ctttccggga
    20041 cggaaagatt ggccacctgg gcaatcgggg tcggcgttcc gtaatagtcc accttgattc
    20101 cgtcaagcag cgagaccgac gcccgcccgg ttcttactct gctgaatgat ttctccagag
    20161 aagagatggt tttttccata ttgtctttga actttgaaaa aatctgctct ttcatgatca
    20221 ttctcctacg tacgttccaa tttttttacc gcaaacaacg ccgcgaatat tgcccttctt
    20281 ctgcagatta aagacgacga ggggcagccg gttgtcacga caaagcgaaa tagccgtcga
    20341 atccattact ttaaggtttt tagtcaaaac atcgatataa cttatctttt taaacaacac
    20401 cgcggagtgg tccaagacgg gatccgcgct gtaaaccccg tccactttcg ttgccttcag
    20461 gatgacatcc gcccggattt ccatcgcccg cagggatgcg gccgtgtccg tggaaaaata
    20521 gggatttccc gttccgccgg caaagatcac aattctgcct ttttccagat ggcgcagggc
    20581 tttgcgttga ataaaaggct ccgcaatttc ccgcatttca atggacgact ggacgcgcgt
    20641 cgcgagcccc ttcgcctcga gtgcgctctg aagggcgagg ctgttgatca ccgtcgccag
    20701 cattcccatg taatcggcgg tcgtgcgctc catgccccgg gcaccggcct gcacgccccg
    20761 gaagatgttg cctcctccga tgacaatccc gatctggatc ttcaggtccg caagagatcg
    20821 gatttcatcg gcaatgtaat ttaccacatc cggatcgacg cctgtggact gcctgcccag
    20881 aagcgcttca ccgctgagct ttaaaaggat tcttttgtac gcggcctttt tcttttcgcc
    20941 cattattttt tgagttcctc gccgagctgg aagcgggcaa aacggcgaat gacgatattt
    21001 tcgcctgtct tggcaatcat gttgctgatc agcgttccga cggtgatgtc ctgattcttg
    21061 acaaatttct gttcgaccag acaaacgtct tcgtagtatt ttttcagttt gccttcaatg
    21121 attttgtccc agatcttttc aggctttttc tcctcgcgaa gctggctgcg gtaaatttcc
    21181 ttttcccggt tgagcgcatc ttcggcaatt tcgtccggac gcacatacaa cggattggag
    21241 gcggcgatat gcatggcaat atccctggcc atggtctgaa aatcggcggt tttcgccaca
    21301 aaatccgttt cacaattgat ttccaccatc acgccgatcc ggccgcccat gtggatgtac
    21361 gaagcaatcg tcccgtcttt ggccgctttg gacgagcgct tggtcgccgc cgaaaggccc
    21421 ttcttccgta aaaaatcaat cgctttctca aaatcgcctt ctacggccgc aagcgcttcc
    21481 ttgcagtcca tcatgccggc gccggttttc gttcttaact ccttgaccat tgttgacgta
    21541 atttccactg tcttttcctc ctggaattct attccctgaa taggctttta cgcaggcgcg
    21601 ttatgaatgc ttctctggag ccggcgcggc cgaaccgttt tccatctcga cgctttccgg
    21661 aatatccgcc tccgcatcct gttggaccgc agccggggct cccccttcct tgttggattc
    21721 cgcggccagc ttttcttcaa accgcttttt tccttccatc accgcatccg caaatttcga
    21781 agagaacagc ttgatggccc gaatcgcatc gtcatttccc ggaataatat aatcgatgtc
    21841 cgtcggatca cagttggtat cgacaatggc gaccagggga attttcagct ttttcgcttc
    21901 ctggaccgtg atccgctcgc ggttgggatc cacgatgaaa acggcggcgg gaagggttct
    21961 catgtttttg atcccgccca gaaccgtttc catcttgtcc atctcttttt gcagcttggt
    22021 gatttccttt ttgggaaaag ccttgaccga atcatcggcg aacatcttgt tgaggctgtt
    22081 gaggcgatcg atactctttt tgatggtgat aaaattggtc agcatgccgc ccagccagcg
    22141 ctgattgacg taaggcatgc cgcagcgcgt cgcctcttcg cgaatggcct cctgggattg
    22201 ctttttggtc ccgacaaaaa ggatttccct gccctgactg accgtatcca cgacaaaatt
    22261 gtaggcggac tggaacatgc ccacggtctg ctgcagatcg atgatgtaaa tgttgttgcg
    22321 ggccccaaag atgtaaggct tcattttggg gttccacttg ttggtctgat gcccgaaatg
    22381 gacgccggct tcgagtaaca gtttcatgga aatggctgac ataacttttt ctcctttgtt
    22441 tttttccgcc actcccgtcg ccttgccggc agacttgaga gtcaagcaac ctgccgtcaa
    22501 tcagggggtg tgtgaaattt tgcggcaatt atcacaaaac catcgattga tcaagcgcaa
    22561 ataacttttt cggcaataaa attaatgttt tttccttaca ggcctcaggt ggtgtaatcg
    22621 gcgttgattt tcacataatc gtaggacagg tccgacgtgt acaccgaata ggacttgtct
    22681 ccacctccca ggctgatgcg gacttctatg ttgccgccct gaaaaatttc cttgagtctg
    22741 gccggcgcca ggttcacggg cgcgtcctgc gcaaagacgg tcatatttcc tatcgataaa
    22801 ctgacttttt ccttttccag caggatgccc gacgcgccga tcgcggagag gattcttccc
    22861 cagttggggt cttcgccgaa aaaggcggtt ttgaccagat tggaattggc gacggaatac
    22921 gccacgcgcc tggcgtccga tttggacttg gcgccctcca ccacgacggt gatgaacttt
    22981 gtcgcgcctt ctccgtcccg gaccacggcc tgcgccagtt cggccagcac atccgtcaac
    23041 atctcccgga acctctgcag cctagcctgg gcccgttcga gcggattgtt cccggccagg
    23101 ccgttggcca gaataatcgc cgtgtcgttg gtgctcatgc aaccgtccac gctgatggca
    23161 ttgaacgtgg aatcgatcac ctgatgaaag accgtattga gcgccttggc gtcgatgagc
    23221 gcgtcggtca tgacataggt taaaagcgtc gccatgttgg gttcgatcat gccggcgccc
    23281 ttggcgattc cgcaaatcgt gatgtccttc gcgccgacga tgcccttgcg gatggcgatc
    23341 ttgggaaatt tgtccgtggt catcatggcc gcttccgcgt cttcgatgcc gaattcgttg
    23401 agccccctga ccagcttgcc gaggccggct tcaattttcc ggacaggcag ccttttgccg
    23461 atcactcccg tggaacaaat gagcagatgc tcttcgggaa ttttcaactg cttggccgcg
    23521 gccgccgaga cagtcagggc gtccgccatc ccctctttgc ccgtcgccgc gttggcgcaa
    23581 ccgctgttgg tcagaacggc ctgcgctatt ccccgtttga ttctctcggc gtcaatcagc
    23641 accggcgccg ccttgaaggt gttcttggta aacagcgcgg cgacttttgc cgggatggtg
    23701 gaaaagatca accccaggtc cttccgatct ccgcttttga tgccggccga tacaccattg
    23761 gccaaaaaac cggggacgct tactttattt gtggattttg tcatgaccgc cactcctttt
    23821 aaaacttaag caccgcaaca tttcttgtat ttcttgccgc tgccgcaggg acagggatca
    23881 ttccgtccga tcttttcggt ctcccgcttc acggtctgat gagccggcgt gtcttctccc
    23941 cggcttaaga tgtaatcctg cttctgtttt tgccgtattt cttcgacttc ttcctctcgt
    24001 tgaatccgga ccagacacag cttttccagc gtatccattt tgatgcggcc gatcatttcc
    24061 atgaacatcg catagccttc acgctgatat tctctcaccg gatccttctg cccgtatccg
    24121 cgcaaaccga tgccttccct caggtggtcc atggacagca ggtgctcttt ccagtgggtg
    24181 tcaatcgcct ggagcatgat gactttcatg agatacgtca tcagatccgg accgaattcc
    24241 ttttccttgc cgcgtaaata ctgccgaaca ttctccacaa tcaggcttcg gatggcatcg
    24301 acggacgtca ggtctttcgc gtttgcgata ttgggacgca atttaaattg ctgaaatacc
    24361 gcatcttcga gcgctttgag gttccaatcc tgaggatgat tcttttcacc gacaaattca
    24421 gaaagcaagt cgccggtaat ttcctcaacc attgcctcaa catcaaccca taagtcctgg
    24481 ccacgcaaga cttttttacg ctgttcgtag atgacctttc tctggttgtt catcacgtcg
    24541 tcgtaatcga gcagatgctt ccggatatca aagttctgcc cttccacgcg tttctgggcg
    24601 ttttcaatgg cccgggaaat gtatttatgc tcgatcggct gaccttcttc gagccccacg
    24661 gtttccatca ccgacgagat ccgctcggcg ccgaaaaccc gcagcaaatc atcctccaga
    24721 gaaagataga agcgcgatga gccattgtcc ccctggcggc cggatcgtcc ccgtaactga
    24781 ttgtcaatcc gtcggctttc gtgtctttcg gttcccagga tatgcagccc ccctaaatcg
    24841 gccacatttt ctcccagttt gatgtccgtc ccgcggccgg ccatgttggt cgaaatcgtg
    24901 acctgtccgg gctgacccgc cccggcgacg atttccgctt ctttttcatg gtttttggca
    24961 ttgagcacat ggtgcttcac ccccgcccgg gtaagatatt tggagagcat ctcggacttt
    25021 tcgatggaga tcgtgccgat cagaaccgga cgtttggctt tgttcagcgc cttcacttct
    25081 tcgatgacgg ctttgatttt ttcctgctcc gtcttgtaga tcaggtcgtt gtgatcctgg
    25141 cggatcatgg gcatgttggt cggaatcacc acgacgtcca ggttgtagat ttttttgaat
    25201 tccgcggctt ccgtatcagc ggtgccggtc attcccgcca gctttttgta catgcggaaa
    25261 taattctgga acgtaatgga ggccagcgtc tggttctcct tttcgatctt gaccttctcc
    25321 ttggcctcca gcgcctgatg cagtccgtcg ctgtaacgtc tgcccggcat cacccgtccg
    25381 gtaaactcat cgacaataat cacctgaccg tctttcacca gataatccac gtcgcgctta
    25441 aacagcgtgt gggcgcggag cgcctgattg acatggtgaa cgatttcaat gttgcgcggt
    25501 tcgtacaggt tctggacgtt cagcagtttt tcaacatgtg acacgccttc ttccgtcagg
    25561 acgaccgttt tgcttttttc ttcaatcgtg taatcggcgt ctttgcgcaa gcggggaatg
    25621 atctggttga ttttgtagta tttgtccgtc gattcttccg acgctccgga gataatgagc
    25681 ggcgtgcgcg cctcgtcaat cagaatgctg tccacttcat cgacgatcgc gtaattgaat
    25741 tcccgctgaa catattcctc caggctgaat ttcatgttgt cgcgaagata atcaaaaccg
    25801 aattcattgt tggtgccgta ggtaatatcc gccccgtagg ccttccggcg ttcgtcgtcg
    25861 tccatgccgt gcacgatcac accgaccgtc agtcccagaa aagaataaat ggggctcatc
    25921 cattccgcgt cgcgccgggc cagataatcg ttgacggtga ccacatggca gcctttgcct
    25981 tcaagcgcgt tcagatacag aggcatcgtc gcggccagcg ttttgccttc gccggttttc
    26041 atttccgcta ttttgccttc atgcagcaca atcccgccga tgacctgcac gtcgaaagga
    26101 cgcatcatca acgtcctgcg cgacgcctct ctggccacgg caaacgcctc cggcagaata
    26161 tcatccagcg tcagtccgtt tttgagtttt tccttaaaat agggagtctt ttcctttaac
    26221 tgcgcatcgc caagggccat cagctctttt tcatattgat tgacttcatg gagcagtatg
    26281 gataatcgct ttaattcacg atcgtttttg gttcctacga tcttcttgag aatcgcgttc
    26341 agcatggacc acctttaaaa aaaccggctt ttaccggtgg attttagcat atttaatcgt
    26401 ttaacaaaaa cattcattta tcgggtcaat tttttccccg cacaccggtg tgcttgggct
    26461 ttcgacccgg cgcgacggct gcattctgtc aattcaaagg cttggattat ttcaagtatt
    26521 ttctcggatt gacgggaata ttattcacaa ttacgccgta atgaagatgc gctccggtgc
    26581 tccggccggt atcgccgaca tatccgatca cgtctcctct tttcacccga accccctctt
    26641 tcacagcggc tcttgataaa tgcgcataac ttgtggaaac cccgtaccca tgctcaattc
    26701 tgacgatatt gccgtcgtcg gaacgatagg ctgcctccaa aacaaaaccg tctgcggggg
    26761 cgataatttc ttttcccaac cttgaagcga tgtcgacgcc tttgtgaaac tccctgtcgc
    26821 ttccgaacgg atttttccgc atgccgaatc cggaggtgac ccacccccga accggccaaa
    26881 gagaaggcgt tgcggccagg agggatttct gctcccggag aaattttaaa agttcattga
    26941 agctcgattc tctttcattg gcttccgcgt tcagccggtc gatgctttcg tgcatgccgg
    27001 aaacgagctt atccctgtcc tcgtcaatga gctgttgcag gcgaatctga tcgttgttgg
    27061 atcctccgat gcccaacggc gtttttccat ccggcccgac ctgctccgac gccagaattc
    27121 gcaatttcct gtcaaattgc ttgatttcct ccattcggtc ggcaaaaccg tcaatcttca
    27181 aggctaactc gtttatttct tttgtctgct gagctgtctg ctgccggagg cgggaaagtt
    27241 ccatgcgatc tcttttaatg ccggcataat cataaacaac atagagcgcc agaagaaccg
    27301 ccagaatggt tccggcacaa aggccataaa ccagtctgcc tgaaagagag atcttggtta
    27361 cagagctctt tctgcggggt atgatcatca gtgtaaaaaa tttatcggac attttccccc
    27421 tgaaaagctg aagtaaaaaa ggacaaacgc gtacaaagtt ttgcatccct aacacaggga
    27481 aaaatcaaag tcaagaataa gtcacggccc tttaggacgg gcaaatgatt attgtcctgc
    27541 gaattcaatg ccttgggtgg ttcaaggggt cttcttttat accgcgcagg gagaatgtct
    27601 tcccggcagc aaatccattt gatactttcg cctgggaagc agctccgtgt tcagtgaatt
    27661 ttttaaatca tcaagcgtcg caatttccat ccccgccgtt aaagctccct tcaaaagctg
    27721 ccgaaatttc tcttcaaaga cccctccctc cacttcggca tgcaccggaa ggatatggtc
    27781 gcccccgtcg ttcatcaaaa cggcgattgc ggccaggtca ttttgagggg cgagctcttc
    27841 aaagcaggga agatcggagg gaatttccgg aaggcgaagc gaatcatgga caaacggcgc
    27901 tttggcccgc gtacagctca gatattcgaa cggatatttc ccgacgattc gcaaaacgcg
    27961 gtcgtcaatc aaccaggaag gggcgccgaa cgcggtcggt tttcgtcccg tgagcttctc
    28021 aaaaccgctc acgccctttt caaaccactc ctcgatccac cattcggatt tcttcggcag
    28081 atcatcctgc cagcggcgat ggtcccaggc atgaaactgt acttcgtggc cttcatcgag
    28141 gatttgacgg accagcaggg gaaaggaaag cgcaatcatc ggagcgggaa gaatcgttcc
    28201 gtaaagggcg gttttccagc cgtacagact gggcgcccgg gtcttcagca ttttttttaa
    28261 aaaaagaggg tttttaagaa gctgcagaac ggcgcggccg gaattgtccg gcccgatgct
    28321 taaaaaaaaa gtcccccgga cggaaaaatc cctgaggacg cgcaaaaggc gcggaacgcc
    28381 gtttttcatt cccgtgtgcg tgtccacatc gatttttaat ccaagcctgc ccatcgaatt
    28441 caatccggtt aaagtattgc cgcctggttt tcctccagaa aagaattgag cgtgagccgc
    28501 agggattcct gcaagccgat tttgggctcc cagccgagca gttttccggc cttctcgatg
    28561 cgcggctttc tggtgtaaat atcctggtag cctttgccgt aatagacgtc cgcgggaatt
    28621 tcaataatat ccgaataaac cgaatcctgt cgatgatcgg gatgctcctg gaacaacttt
    28681 ttgagcagag ccgccagttc ctttaccgaa cattcatttt cgggattgcc gatattgatg
    28741 atttgattcc tgcaaatatc atccttgttt tccagaatct tcataagcgc cgcgatgccg
    28801 tcgtccacat aggtaaaaca gcgcttctgc ctgccgccgt ccaccagatt gatcggacgc
    28861 ttcaaaagca gctcggcgat aaattgcgtc acaacgcgcg agcttccctc tttggcggta
    28921 tccagcgaat cgagcctcgg cccgatccag ttgaagggac ggaaaagcgt gaattccagg
    28981 ctgcctctcg ttccataggc ataaatcaca cgatccagaa gctgtttgca gcaggaataa
    29041 atccagcgct ccttgcgaat cggccccacc gacagatagc tttcgtcttc gtcaaactcc
    29101 ggatcggcgc aggcgccgta gacttccgac gtggagggaa agacaacgcg cttgtggtat
    29161 ttgacgcact gcttgacaat gttgatgttc atttcaaaat ccaggtgata gacgccgatg
    29221 gggtcttcca cgtaagcctt gggggtggcg atcgccacca gcggcatgat gacgtcacat
    29281 tttttgatat ggtattcaat ccattcccgg ttgatggaaa tatccccctc cagaaaatta
    29341 aagcggggat tgtgcagggc ggcgccgatc cgctccgttc ccagatccat tccgaacacc
    29401 tgccagtccg gcttctcggc cagaatccgg ttgaccagat gatgcccgat gaacccgttg
    29461 acgcccagta ttaatatctt catgtcagtt tcttcacctt tccctgttga aaatagtccc
    29521 tgatccccgc atccttccat cttgccccgg atgcttcgat ctccagaagc cttatagcac
    29581 catcaccggt tttcacaagc acggattgcc cgtcaaggat cacgtcgccc ggtccgcctc
    29641 cggctttcga cgaatccggc ctggcccgcc agaccattat tttttcgccg ctttcggcaa
    29701 aggcatacgc gcccggatac ggctccgtca cggcccggat caggttgtag atgtggaggg
    29761 cgctctcact ccagaaaatg cgcccgtctt ccggcttgcg gccgccgtaa tagcttcccc
    29821 gggaaaggtc ctgcttgatg cgcggaattt ttcctttctt catttcgggg agcaactcgt
    29881 cgagcagccc gcctgccgcc ttgcagagct tgtcgtacag cgttcgcgcc gtatcctcaa
    29941 aatcaatttt cacggctttt tggccgacga tgtcgcccgc gtccggtttt tccaccatat
    30001 catgcagcgt gacgcccgtc tcttcctctc cgttgacgag cacccagttg accggacacc
    30061 tgccccggta agacggcaga agcgagccgt gcagattgaa agcgccgatg cggggaatcg
    30121 ccagaacctc cggacgaacc atgttccggt agtaaaagga aaaaaggaca tccggctgaa
    30181 ggcgttttat tttttcaatc gtctggggcg agttgaccgc gtcggtcgcg cagtcaatcc
    30241 cctgattttc cgcccagtcc ttcacggagc cgaaccagca gttttcctga gggtcgtctt
    30301 gatgcgtgaa gacggcggca atgtcaaagc cgtgccgccg gagagcggca agacccgcca
    30361 gccccatatt gtgataggcc aaaacaatcg ctttcacgga cgatcctcgt gcagaccgta
    30421 aatgcgttca ataacgaatt ccggcctttt gcggacttcc cgaaagatcc ggccgatata
    30481 ctcgccgatc acgcccaggg caaaaaactg gagaccgacg aagacaaaca aaatcgcaaa
    30541 cagcgaaaag atgccttccg ccgcccagcg cacaccgtaa acgaggcggg cgaccgccag
    30601 aaacgcgccg aagcacacgc ccagaagcga catcagaatc ccgccgtaca tgatcagctt
    30661 aatgggaaaa tccgagaagg acgccaccag atcaaattgc aggttgatga gcttccttaa
    30721 cggatagttg gatttgccgc cgaaccgctc ttcgtgcgcc acttcgattt ccgtcacccg
    30781 cttggcaaaa acggtggcca gggcgggaat aaaggtcgcc tgttcgtggc aggcgagcat
    30841 ccgctccacg accgggcgac tgtacgcccg cagcatgcag ccccagtcgc gcatgctgac
    30901 gccggtgatt tttcgcgcga cgacattgat cattttggaa ggaaggatgc gcaggaaaga
    30961 atctttgcgc cccttgcgga tcgtgccgac gacatcgtat cccccctctt ccatcacacg
    31021 gacaaggttc gggatttctt caggcggatt ctgcagatcc gcgtccatcg tcagcaccat
    31081 gtcgcctcgc aggatcgaaa atcccgccat gatcgcggcg tgctggccgt aattgcgtgt
    31141 cagctccact acccggatcg acgggtgtga cgtaaattcc ttcaaaagcg caagcgaccg
    31201 gtcgcggctg ccgtcgtcca ccaggataat ttcaaacggt ttggccagag actccatcac
    31261 gggcagcagc cggcccatca gcgccttgag gttggcttcc tcattgtaca ccggaatcac
    31321 caccgagatc cgcatttcag gcatttttca aaatctcctt tatggccccg caaacatagt
    31381 gaacgtcatt ttccgtcata tccggaaata aaggcagaga aatgattcga tctgcagcca
    31441 ggtttgtttg tggaagagac gccgccctga cgccgaatcg cttcttcaca tacgacaatt
    31501 tgtgcgcagg cggaaaatgc aagccgtagc cgatattgtg atccgccagt ttctgcatga
    31561 aaagatcccg ggacatcccc gtgaccttga ccacaaacaa atgccaggca tgaacgtggt
    31621 cataggaagg aacttcaggc aaatcgaggc cctctgtccc ccgcagaccg tcgaagtaaa
    31681 gccccgccag aactcgccgc cttgcattga gctcccgcca tcgctccatc tgcgccagcc
    31741 ccagcgccgc cagcaggtcc ggcatgttgt acttgtagcc cggctctgcg atgtcatagg
    31801 aaggattccc ccccttgccg taccgtttcc aagcgtctct ttctattcca tggaatcgcg
    31861 tcaaacgcaa tttcttttcc acagcggcgc tgttcagcgt aatcatgcct ccttctccgg
    31921 tcgtaatgtt tttgatcggg tgaaaggaaa agattgcggg atggccaaag ccgcccgcgt
    31981 gcgtcccttt gtaggacgtg ccgaccgcgt gggccgcgtc ttcaatgacc ttcagggaat
    32041 atttccgggc cagccggttg actcggtcca tgtccgccgg agcgccggca aaatgaacgg
    32101 gaatcatcac tttggtccgt tcggtaattt tttcttccat cgcctcacag tccatgttga
    32161 gcgtcccgta atcaacatcg acaaacacgg gctttgcctg acgaagggcg atcaggttga
    32221 cggtcgaggc aaacgtcatc gacggcgtga tcacttcatc gcccggcccg acgttcaggg
    32281 ccgtaagcat cagatgcatt ccggctgtgg ccgaactgag cgagacggcg cggggagcgc
    32341 cggtcagcgc gcacaactgt tcttcgaacg ccttgcaaag ggcgccggtg gtgatccatc
    32401 cggatttcag gcaggccgcc acggcgttaa tctccgtctc cccgatggac gggcggctga
    32461 acggcagaaa atctttacga atcttcatag ggcatcaatc ctttggctga tttcgaatgc
    32521 gcagcagata aaacgcgccg ttttcccaga gcatatccat tcctgcaaac tgttcccgca
    32581 ggtctttgag ccttttttcg tgctgtgtaa tgcagtaaac ctctttttcc tgcctgcatt
    32641 ttgcaaagaa atcctgtctt gaaagaaaat atttttttct ttcggcctcc ggcagttttg
    32701 caatgccttc actcaattcg ccaaaatcct cgacaatggg cgttcggatt ttgttgtaaa
    32761 aatcaattcc gtagaaattg acgcggtact gatacaaaag ctttccttgc ggcacgtacc
    32821 gcgcaatcgc ctctttcgca accagcgcgc tgcggtaggg atacaaaaaa tcattgagag
    32881 gaaagagaaa acccgcaagg aaaatgcagc aaagcagata cagggaggga aaccagcccg
    32941 cgccctttcg gcgggcaatc caatccggaa gaaaaatcat caaccccgcc gccagaaaca
    33001 ccggcaaaag gtagatccac cagaggtctg aaatcatgat gaccaacccc ctggaaggat
    33061 catgatgcga tttgacaatc ggcgacaaaa gaagagcggc gcccacgatc aggaactggc
    33121 cccacgccag aagactgtag aggattttcc gtcttccggt cggctgcgcc ggagcttcgt
    33181 cgtagccgcg aaagatgcag cccgcaaaaa gcgctgcggg cagaaacacc ggcgcaatat
    33241 aggtgacaag tttagaagag gaaaccgtat aaaagagaaa aataaataaa aaccaaacag
    33301 ccagaaaccg gttctcctct ttcctgaaca actgctcttt ccattttccc ggctgccagg
    33361 cgcggatcag aaaaattgac cagggaaggg tgccgccgat caacacgggc agaaaataat
    33421 aaaacggctc cgtcttgccg tgcatctgcg tcgtaaagcg caggaagtgt tcccgcacaa
    33481 agaaaaacca caggaaatcc ggattctctt tttgcgccag ataaagccat gggcaaacca
    33541 cggccagaaa aatgataatt ccagcggggg aaatcaaccg cgggagcaaa cgccagcgac
    33601 gttcccagac gagccagatc cccagaacag caaaaggaaa aacgacgccg atcattccct
    33661 tggtcagaaa agccaaggcg catgcgaaat aaaacccgta agaaagcagc cttttctttt
    33721 cctccgtcac ggacaggtat cccagccaga tggccagaca aagaaacagg gtaagcggca
    33781 tgtccaggat attgattctg cccagcgcaa acgggaaggc ggaaatggta aggacggccg
    33841 ctgcatacag gccggttttt tcatcacgga agcgccgtcc gatccaaaat accagcagaa
    33901 tacaccccca ggcgcaaaga ccggaaaaca gccgggcaga aaagtcattt tctccgaaga
    33961 ttttaaaggc tgccgccgtc acccaggaaa ccagcggcgg tttttccagg tagacggtat
    34021 ttttgatttt gggggttaca tagctgcctg tttcgttcat tgcggaggca atcagactgt
    34081 agcgcacctc atcaggctcc atctgcggca tgacccccag cagggcgaca taaagaagga
    34141 aaggcacgat ccacaaaacc gcttttttct tcatcgttta ttcactcctg atacagcgtc
    34201 gtgaatttct aaaaccgttc ggctctccgg ttaagctgga gcctgatcga tccggatatc
    34261 ggaatgattg ccggcgggaa aagcctcttc cggatcgtcc atgtcttgag agctttgaat
    34321 aacggccatt tttgtcattt cgaccgaagg gagaaaccta tcaatcttca caagcattaa
    34381 gatttctcgt cgctcacgct cctcgaaatg acaaaattca aatctgacga cctcgtaaaa
    34441 agcaacccac accgtcaccc cggcgaaagc cggggtccat aactcattgt aattactgga
    34501 taccggcctt cgccggtatg acgaaaagag tgcgaaattg actttttacg aagccgtcaa
    34561 atctctctaa tcgatccggc gcatggcatt catcttcacg atcagatcta ttcccttcag
    34621 cggcgggcag gttcggcaaa gttcgcaagg aatatagaca aaatacaccc gactgtcaac
    34681 aaaacaatag cgtccggcgg gcgaaaacca ttttgtcatg gctgcatgtt ttccttgtgc
    34741 aaaaacgccg atgcgtgata gtttttttca aaatttttaa agtgcgctat gaataaaatt
    34801 atcaatcgct acatattccg ggaaatcgcc cttcccttca tcattattct ctttgtcctg
    34861 acgtttgtgc tgctgatggg caaaattctg cagatcatgg acctgatggt caataaaggc
    34921 atccgggttc tggacatcgg gcatctgatt gttctgatca tgcccaattt catgctgttt
    34981 accatcccca tcgcgcttct ggtttccatc ctcattgcca tgggccgtct gtccgccgac
    35041 aacgaaatca cggtcctgaa ggcgtccggc gtcagcctgc ttcagctttc ttatccggtg
    35101 gcggtcgcct ccctgatggc ctttgcctgc gccatggtca tcggatattt tctggtgccc
    35161 cagagcaact tcgcctccaa gcggcttctc tttgaactgg cctcgcaaaa tgccggcatc
    35221 ggcatcaagg aaaaagtttt caacgccgac ttcaaaggac tgctcttgta tgccgatacc
    35281 gttccggccg acggggaatc gatggaaaac gtgatcatct cggacagccg agtcctgggc
    35341 gaacaaaaca cgatcctcgc aaagaaagcc ttcctggtgg ccgatccgaa gctgatgatt
    35401 ttgaagttgc gccttgaaga cggctcgatt cacaccgtgg gcaaagattt gaaaaattac
    35461 cggaaagtgg attttaaaag ctacgacatc aaccttgacc tgtcgtcaac gttcgcgtct
    35521 tttaccgacg aatccaagtc cagcaccgag atgactctgt cggagcttct ggagcgaatg
    35581 aaaaaaccgg cgctggcgga tgcggtcgtg cgggaactgg ccattgaggt gcataaaaag
    35641 ttttccatcc ccctgtcctg tcttttcttc gcactgctgg ccataccgct gggcatcacg
    35701 caccatcggg ccgtaaaatc ccggggcttc gccgtcggcc tcatcattgt ggccgcctat
    35761 tacctgctgc gaatcggggg agaagccctg gtggaaaacg gcagaatgga cccggcactc
    35821 ggcgtctggg cgccgaatgt gctcttcgcg ctgctgggtg tttttctgtt ttacctggcc
    35881 caccgggaga tttccgtctc ccagacgctg atcgcgattt ttcaaggcga aaagccgaga
    35941 ggatgaactc gtgaaactga tcgatcaata cattctgaaa gaatttctca ggtttttcct
    36001 gatcacgttt gtgtcgttca tcatgctttt cctgattatc gattttttcg agaaaagccg
    36061 catgttcatg agcaaccatg ccacggtggc ccaaatcgcc tcgtatattt tgtattccat
    36121 cccctttgtc atttccatga ccctgcccgt cgctgtcctg ctcgcgactt tgctgaccta
    36181 cagctttctg tccaagcaca gcgagattac ggccatgaaa gccaacggaa tcagtcttta
    36241 ccgcatttct cttcccgtac tggccgtcgc ggcggccgtg tccgtcttcc tgttcttctt
    36301 cacggagatg atcacacccg ccagcgtcca gaaaatggaa catattatca aggtagaagt
    36361 gcagaagcag caacccctgg ggtttttcaa gcagaatgaa atctggtacc gcagtcaaaa
    36421 tgccatctac aattttaaaa tgtttgacgt tgagaaaaat atcctgcacg gcgtcaccat
    36481 caaccgtctg aaccccgact tcacgatgaa aacccgcatc gacgccgaac gggcggaatg
    36541 gaaaaaccaa tcatgggtgt tttacaatct gctcgtcacg acctttcagc ccaacggccc
    36601 cccgtcgctg gaatggtcga aagagaaagt agttgcgctg ccggagggac ccaacgactt
    36661 caagatcatt cagaaagacg cggaaaagat ggggtatttc gaactgcggc gctacgtgaa
    36721 gaagatccgg gcggaagggt acgacgtctc gcgatacctg gtcgatttgc agggtaaaat
    36781 cgccttcccg ctcgtctgtc tgattgttgt tctgatcggc atgtcgtttt ccgtccgctc
    36841 ggagcgaagc ggcggcatca tgcagagtct ggcgcttgga ttattcctgg gcttttccta
    36901 ctggattgtt cacgctttca gcatgtctct gggcagatcc ggaacgctgc ccgttttcct
    36961 ggcggcctgg accgccaaca tcctcttcgg cggcctggga gccgtgctgt tctatcggat
    37021 cagaacctga cgg
//
LOCUS       DAOSRM010000007        34812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1367_length_34812_cov_111.622695, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000007 DAOSRM010000000
VERSION     DAOSRM010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 34812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34812
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_34812_cov_111.622695"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..986)
                     /locus_tag="PLA58_01640"
     CDS             complement(84..986)
                     /locus_tag="PLA58_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOR61528.1"
                     /translation="MSLKKVFSIGCMALLLMGLGFISPAYSDVDGKIDWVDGYVSAVG
                     YGGAAKGNMALSRPLSRRAAIGDAYRNLLEIIKGVKIDSVTTVENFMVTQDIIRTQID
                     GVVKGARIHKEKYESQPDGSLLATVEMRVCISNCPGTTTLVQALNVEQKDDAPFVPSK
                     RLQDIPVVQTPPPPKEYTIVYDKTRPVTGMVVNLEGRIFERVLLPVIIVEEVGDSPVT
                     IYSAKSVKPAVVRTHGVVRYADSIDQARNNTHLGDNVMIIPASKITKENMIVLRGEAA
                     RLIKETTAHGNDYLSEARVVISAQ"
     gene            complement(1084..>1741)
                     /locus_tag="PLA58_01645"
     CDS             complement(1084..>1741)
                     /locus_tag="PLA58_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61529.1"
                     /translation="GSSGAGFVEIKGVKTGTAVTVGEKELLTNGTLKSLSGWTIHRWM
                     QPTGDRGEVTQESDGIKFRNTVGNTRMGIMQTVNADVSGAQRLILKAVVKAVEQNLTG
                     TGWQGREAPVAVMVTYTDVAGVVRNGLGSMANPTESQTNRMFWHGFYYANPTGNSQTW
                     HGTKVSQGSWHTYEVDLMSLNPKPKMIHAVGAEGSGWPTREGRISSISLKFYGTISGR
                     "
     assembly_gap    1742..1841
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1842..2851)
                     /locus_tag="PLA58_01650"
     CDS             complement(<1842..2851)
                     /locus_tag="PLA58_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61530.1"
                     /translation="MKKSIPSCLLAILVCLAVFSLNAEARKPEITRHDAQYLDRQISI
                     NLQWKSSVEIVSVRVAAGKEVKEIKVDPYDNKKTRLGYLGEVNVVLETDPMVSQESIG
                     YTIQIQDEDGQKSNLVTGKVAIPATAYSQPPREHRDSWGKEHLAGTEKSGKKDMIDQL
                     REVATVLAAPPVILDVTVNNPGTGNVTFKTKATHTVGMREITFRVFDASGKQVDAQQI
                     SATGKLWEGTSKDFSLPPGSYSVIAQAVDASGGTSRESRASFAIRGAGLPTQQPATPP
                     VTPPVTPPVEPPVTPPVIPPVMPPVEPPVTPPVVPPVTPPVTPPVTPPVTPPVTPPVT
                     PPV"
     gene            complement(2910..4013)
                     /locus_tag="PLA58_01655"
     CDS             complement(2910..4013)
                     /locus_tag="PLA58_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61531.1"
                     /translation="MGKNKKSMVVAGVVGSLLVLFFLTSCVSTSGTAMKTHQKGMSSI
                     TEEEYFTTKKPVDRAYIGCTWSKQFGPLEDPVAEEIEVKKEKSLSGIQQDFAYNLGVG
                     LGGQTIAGPAAEIGASGGSVEKAKLSGLEIITPVSIADIAFEPGLPYITEALRLANFR
                     IADEKSNKAGLNVGAGTAIGSVTATAGAGTQARRGTEGEGLVVAYKLHMIDSETYAKS
                     ESGNRTLELDKTVEFSKEGLFAKASLQLIEPGSGKSLPRNVIWSCPRAHAKSRDTVAA
                     WVVELKSTDPRRKKTTIAFPAWPAIEDCQNYSGVIFSRIDPRTDKIIRQKISITLIDA
                     EVSDAMKPKAWNTRIALVDESFNIKLVSPKDLE"
     gene            complement(4152..4226)
                     /locus_tag="PLA58_01660"
     tRNA            complement(4152..4226)
                     /locus_tag="PLA58_01660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4192..4194),aa:Gly,seq:ccc)
     gene            4459..6045
                     /locus_tag="PLA58_01665"
     CDS             4459..6045
                     /locus_tag="PLA58_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOR61532.1"
                     /translation="MSKQLQKEIERRRTFGIISHPDAGKTTLTEKLLLFGGAIQMAGA
                     VKARKASKHALSDWMSIEKERGISVTSSVMKFEYAGCEINLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDGAKGVETQTQKLMEVCRLRNTPIITFINKLDRDCLAPLDILTDIEDK
                     LQLECSPLSWPIGMGKRFRGVYNLYDQKLHLFTPGQSKRTDEGVVISDLSDGLLDELL
                     DSQAAELREEIALLTGAANPFEIREYLKAGQTPVFFGSAINNFGVRELLDAFVEIAPS
                     PVARPTTTRQVEPDEDDFSGFVFKIQANMDPAHRDRIAFMRICSGKFERGMRVVHHRI
                     HKEISLSNATIFMAQDRTNVETAYPGDIIGIHNHGTIKVGDTFTQKEPLKFTGIPHFA
                     PEHFCRVRLKDPLRLKHLQKGLMQLSEEGAIQVFKPLWGSDNILGAVGALQFDVTMAR
                     LKDEYSVYADYEQADYAAARWVTCDDAGKMEDFQKKNSMNLATDTEGNLVYLALSDWR
                     LSHVMEEWPQIRFHKTMEKS"
     gene            complement(6126..6530)
                     /locus_tag="PLA58_01670"
     CDS             complement(6126..6530)
                     /locus_tag="PLA58_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOR61533.1"
                     /translation="MKAVFDTNVLIAAFLTEGLCSGLMIRARKQAFSLVLCDDIIGEF
                     EGILRKKFKITPADVSEISAIVSEAASEIIHDLSPVPSICRDPEDNIIIACAVDAQAD
                     YIVTGDEDLLILKRYKGIVILNPRNFEALFAD"
     gene            complement(6527..6748)
                     /locus_tag="PLA58_01675"
     CDS             complement(6527..6748)
                     /locus_tag="PLA58_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOR61534.1"
                     /translation="MMRSVLSVSLPEKMASELDAIARATGRNKSDIVKESLGVFLWEA
                     RFRRMKKTLGPKAKASGLITDDDVFKVIS"
     gene            complement(6847..8709)
                     /locus_tag="PLA58_01680"
     CDS             complement(6847..8709)
                     /locus_tag="PLA58_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOR61535.1"
                     /translation="MSNLLVDSRDVRYVLFEMLEVDSCLQYEKFSDQDQALYEDTLRL
                     AEQISVEQFYPVNSEGDRVGVHFDNKLGVVTVPACFQQPYRAISEAGFIGVSADPAFG
                     GLGLPLSVSVACKEYFCAANGCLTFYTLLSGSAAQLVYSFGSKEQKQTYLPKMLSGKW
                     SGTMCLTEPNAGSDVGNVKTKAVRREDGAYEITGQKIFISGGDHDLTDNIVHMVLARI
                     EGDPPGTRGLSLFIVPKFISEPDGTPGRRNDVVCTGIEEKMGIHGLSTCALAFGENNS
                     CVGYLLGQERKGMRQMFKMMNEERLFVGLQGLSCSTAACMHAIAYAGTRLQGVHVTGM
                     LDPNAPQVPIIEHPDVVRMLLWMKSYVEGMRMLTYFLGKNLDVEAVDQGEKGREAKAL
                     TELLIPLCKAGNTDASWLVTAEAIQVYGGYGYCRDYPVEQYARDCKILSIVEGTNGIQ
                     SVDLLMRKILMDPDERNLNVFRKLTKQTVNTAREAVEEVYITPVARGLVELDKMVGFL
                     KARMGQGKIAGIFAQATPFQKAMFLLCLGWMHLWNLSLAASQLKKTTGRAVADCSFEN
                     IRNDSEACFYYNKIMTSRFFIAAEFPKFFAYVESILNDEPALKAAGSDLFPDRP"
     gene            complement(8760..10037)
                     /locus_tag="PLA58_01685"
     CDS             complement(8760..10037)
                     /locus_tag="PLA58_01685"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOR61536.1"
                     /translation="MANENTAKSGHQKTAAGRRLSKIMFNDYMNLHQKAGEGAFVVWM
                     AIVVPAELFSGFENVVYGVPESHAAMSAGKGVGPLQCEKAEKMGYSMDLCSYARIDLG
                     TAFDGGKDSPTMGLPKPHLIVSNNNNCSLLVKWFDIYHRTWGVPHFILDVPFCYEPQN
                     ARDLDYIVTQYRDLIRTIENLSGQTFNIERVREALENTRDALANWKRFLKTAASRPAG
                     LTAFDSFVQMAPYLTSRGTKTLADHYRLLAEEAEDNIAKGIYPVPNERYRLLWDNIAP
                     WHQLSRMSSRLASLDVNLISASYTYCIGTVEGELDLFAYDGGDPLVYLARMQNSSICP
                     HGMSLRFRAMRRAIEDWGVDGVIFASNRSCKVYSIMQMDMLKRVTNELGIPAVMIDVD
                     HADMRKYSEEQSFTRIEALLEVIDARRKEAKLS"
     gene            complement(10048..11157)
                     /locus_tag="PLA58_01690"
     CDS             complement(10048..11157)
                     /locus_tag="PLA58_01690"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOR61537.1"
                     /translation="MDNIKRLKDQGRKVIGCFPLYPPLELFHSMGLAPVTLWGLRDSL
                     PRVAESDRHIQPYACSVARRLMEFLLKENSSALDGLFMYNACDTLRNLPEIIDCGLAD
                     AGRPPLPIFKMHIPAVPPDQTDSRPYLRASIEGLIGELEKTCNVTFAPDKFRRSISLY
                     REMRELSKMLFASVADGSMSYGEFSRIVTEGWFIPVEDQLLVLQSALRDNFRKKTKPS
                     PESPRVVVSGILPPPLPMIRDLENAGITVVGNDVASQNRALHYSPPAVDDPAEYYIDF
                     YHHHYPCTTLLHSADRRLPAIRKLVSDTGARGVVFVGEKFCEYEYFEFPYLEQILKEM
                     GIRALLLDISLDDDENTAAYTTRIETFAELMHSTV"
     gene            complement(11234..12754)
                     /locus_tag="PLA58_01695"
     CDS             complement(11234..12754)
                     /locus_tag="PLA58_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOR61538.1"
                     /translation="MNCAKNREMPALICEDRTVSWEQMWDRTARLGNGWMDLGMTKGD
                     RLAIYLKNSIEFSETFISTYHSGVIKTPVSSGLKAAELAHQLNDSGACAVVTSPELLD
                     VLIAAKPNVPALKHVIVTGSNVTKDHISYEQLIRESSPEDRGMEVSPYDIDMLLYTSG
                     TTGFPKGAVRGVMEDYHTGVTVCIDWRIRMGDVQMVVIPQYHAGACAWFLATLISGGT
                     QVIMPSYVPEKVLQNIEKYRVNWLMMVPVMYDWLMLLPQEVLNKYDVSSLRTLISGGA
                     PLHTPTKIKIKETFKNAELNEFYGSTELGVSTCLRDEDQLRKERSVGKPGHDLELKLF
                     NEEGMEVRQGEPGILYSRGLGGFRGYWENEAATNKAFLEGGWATVGDIARQDEEGYYY
                     IVDRLNDMIITGGVNVYPVEIEEVLHRMPNIQDVAVIGVPDEKWGEAVKAVVVPVKNA
                     DLSEEEVIGFCKDNLSKVKIPKSVDFVDAIPRTLTGKILKKDIRKKYWSDNTVQVS"
     gene            12964..13650
                     /locus_tag="PLA58_01700"
     CDS             12964..13650
                     /locus_tag="PLA58_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR61539.1"
                     /translation="MPKEMKCHMVRLKKSSAFEAKNLPMKEKKTFNKLKQQEWEVRRN
                     LIMDAAQRVFASETFNKANMREIAREANISPGSIYNYFENQEELFLAASLRGADRLIK
                     VFSDLVAEDHPSIQTAAVAYIDFISKNYEYLRMVQHSMLYAQFTSQESIDQLVETARK
                     LFEQFDMIIQQYISSGNVRKFSHLFFVALNGILFSYGRFPDRTSDEVLTYMKELASLL
                     GELLEKYQPK"
     gene            13705..16158
                     /locus_tag="PLA58_01705"
     CDS             13705..16158
                     /locus_tag="PLA58_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR61540.1"
                     /translation="MNQQPVKKNRGEKNTGPNPEEQGSAGTIGGSQKRARSMMKSGVA
                     AASQAERLRARQNLRESKKRYRLLAENLTDVIWVLDMRLKHVYVSPSVENLRGFTPEE
                     AMKQPLEEVLTPDSYRKVLDILSRELSLETGGQRRQRGWTQNIELEMLRKDGSTIWTE
                     VKISLLYDEHNRPQEILGITRDITERKKAEEALKKSEEYYRAIFEHTATANMILAEDT
                     TVLSVNANFEKTMGYSKKNVENKMSWTQFVVDEDVEVMKQRHVQRRIHPESVPSTYEF
                     RGLTQSGDIRSFFLSVDMIPGTTNSVASLFDITDRKKAEDDLRQSEERFRDMARLLPE
                     TVFEADLNGTFTFVNEPSFDRFGFTRNDMKKGLSLTDVLAPDDRERARANFQKIIRGE
                     SSGLNEYVAQKKDKTRFPVMVHTSPIFKEGKTVGFRGFLIDISEKKAMESQLLRAQKL
                     EAIGTLAGGIAHDFNNLLMGILGNISLMLMHIDQGHPFHERLKNMEEYVQKGSDLTRQ
                     LLGFARGGKYEVRTTNLGKFAARSAEMFGRTRKEISIHHTAEKDLWHVDVDRGQMDQV
                     LLNLFVNAWQAMPGGGNLYIAVENTELGAQDVAGFGVTPGRFVKLTVRDTGVGMDEST
                     RSRIFEPFFSTKERGRGTGLGLASVYGIIKNHGGFIGVESEIGVGSTFTIHLPASEEK
                     TEEEDQPESKPDRGRGTVLIIDDEEMIVDVGRQMLEALGYIVLTAGGGREGIDIFQRN
                     AKSVDLVILDMIMPDLSGRETFEALRRRNPAVNVLLSSGYSLDSQAKDMMAEGCKGFI
                     QKPFTMAELSRKIREMIEK"
     gene            complement(16246..17592)
                     /locus_tag="PLA58_01710"
     CDS             complement(16246..17592)
                     /locus_tag="PLA58_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR61541.1"
                     /translation="MDQTTMEADVVVVGGGAAGLMAAGRAAERGARVLLLEKMEGCGK
                     KILVSGKTRCNLTNAADLESFIAMYGPNGRFLHGAFSRFFRSELLAFFERYGLATKVE
                     RGGRIFPVSDDARDVVRVFQKYLTEHRVRRLLHARVTSVALRPDSLFVVETTGGRYLA
                     SSVILAAGGASWPATGSEGDGYALSAALGHTIVKLRPALVPLIVREQALARSMQGVSL
                     RNVRATAFQGEAGTIDAALAPPCDYGRGESKKPRPPLIESRFGEMLFTHFGLGGPIVL
                     LLSLAVVDALERGPVSILIDLKPAVTREQLGKRLQKDFNDFGKRKIATIWKDYLPAKM
                     VGPLAALSGVDENKPANQVGAAEREKIIDVLKALRFNIQAPLPLAKAIVTAGGVALDD
                     IDPRTLMSKKVPGFYFCGEVMDIDADTGGYNLQAAFSTGYVAGENAAAFAAAKNRS"
     gene            17723..18778
                     /locus_tag="PLA58_01715"
     CDS             17723..18778
                     /locus_tag="PLA58_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraprenyl-beta-curcumene synthase family
                     protein"
                     /protein_id="HOR61542.1"
                     /translation="MRTPTHILSLTTRMILQVRPAAHGFLHRWKERARRIPDPELRRQ
                     ALASIETKTFHCEGGALCGLLAGGFFREAIQFIVAYQTISDYLDNLCDRSTSQDPEDF
                     RALHEAMLHALTPEAPTADYYRFRREHDDGGYLAELARTCQTILGGQPEYRRVAPSIR
                     ALARLYADLQVHKHVRPDEREARLKKWFENHRAGLPEMAWHEFAAGAGSTLGIFCMVS
                     QLIRKQDPLPVVEQIHKAYFPWVQGLHILLDYLIDQEEDRRGGDLNFCSYYENHERLT
                     FRLCHFYSMAHASVADLPDAKFHHLMISGLLSIYLSDRKVSRQKDVRAIARKLLALGG
                     AETFFFYLHCWVYRRLS"
     gene            18969..19349
                     /locus_tag="PLA58_01720"
     CDS             18969..19349
                     /locus_tag="PLA58_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61543.1"
                     /translation="MKKALVCATGIVCLLAVFAFAAEKPSARSTQSVLPKTATMNARG
                     KVVEISEKAVRIERSIKGNAEVMEFALENPAADIAVNDFVKIDYTIKDGKLTASRVAK
                     PKAEKNYRKNGSKQVKEKSAPVTK"
     gene            19435..19947
                     /locus_tag="PLA58_01725"
     CDS             19435..19947
                     /locus_tag="PLA58_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61544.1"
                     /translation="MKRLVLFVIVAVFALCTVAMAQVSTKDEVQSGGTKISDADKAAV
                     KDAQKAAKEKRAAKKAGLVKKEKVKAPEAKKGVSTVEEVQSGGTKISDADKAAVKDAQ
                     KAAKEERAAKKAGLVKKEKVKAPEAKKGVSTVEEVQSGGTKISDADKAAVKDAQKAAK
                     EAKAAKKAAK"
     gene            20110..21654
                     /locus_tag="PLA58_01730"
     CDS             20110..21654
                     /locus_tag="PLA58_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR61545.1"
                     /translation="MLKKLLSKIIDYNNNLTLRVRLVALTVAGLALTMAVWGLIHLTA
                     LDRILVDQQGKRLEGVAETVSTFYQHFPTRQGIATLDSTLKDHIQTDVRLARIDIMEI
                     RRNAVNYVAGASRVPYEWPDHLVAETGAKGKPLSMKLETEGGPSLGLLYPVSGDTRDS
                     RIVVGIIAFSRANAEIMSRARQLLLVSSAGLLLAILLLLGVSYGWLIGRPLEMIIETI
                     DEFQKGRYVERIPIVRRDEWGHLADHFNQMADEIQNVMERNENLTRHLADRVREETLN
                     VIHLQKQVDDLKQLTALGHLTANLAHDLGTPLHSIAGLARLMLEREGWPPDVAHKLNV
                     IVEQTQRLDHVIQNVRKATRLPEPHLELMTVHKILSETLPLVEPLIQKNRITIEVRLE
                     RSVASLYADRYRIQTALLNILQNSIEAMPGGGVIRISAQDDRAARTVVIAISDNGPGI
                     EADLLQRVCDPFVSSHSGEGMRGLGLAIVRDIIKAHNGRMDIQSVPGSGTSVILYLRM
                     ADDATE"
     gene            complement(21658..>22533)
                     /locus_tag="PLA58_01735"
     CDS             complement(21658..>22533)
                     /locus_tag="PLA58_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOR61546.1"
                     /translation="IARVADSPASVLIEGESGTGKELTARSLHSMSSRRQKPFVVVHC
                     GAIPDNLLESELFGYEKGSFTGADHQHVGLIESAGGGTIFLDEITEMSTALQSKLLRF
                     MQDGEVRRIGGHDVRHVAVRVVAAANKNIDEEVAAGRFRLDLLYRFIVRLLIPPLRER
                     RSDIPLLIETMLKKLGYPDVRVSPEAMETLMGYDWPGNVRELENVLRQTLLLSPFVVI
                     LPENLPDRFRTRAVVSAQAGPPEWTPLEEAEREQIVQTLKKTEWNQSRAAEILGIDRK
                     TLRTKIRRYSLQEEK"
     assembly_gap    22534..22633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22634..23144)
                     /locus_tag="PLA58_01740"
     CDS             complement(<22634..23144)
                     /locus_tag="PLA58_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR61547.1"
                     /translation="MKARIIVIDDDAVACEFLQEVLQRDGYDVSSFTSASAALKQNLA
                     DYDLLMSDIRMPGMDGLQLLDEIQKQWPNLPVILMTAYGSLETTMEAIRLGAWDYISK
                     PFSPEDCRALVKKVLEVRELRKHRSSEPLEAAEEAKLIGSSPVMVEFYKQIARVADSP
                     ASVLIEGESG"
     gene            complement(23179..23628)
                     /locus_tag="PLA58_01745"
     CDS             complement(23179..23628)
                     /locus_tag="PLA58_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOR61548.1"
                     /translation="MALVFDEIQIGDVMPPYTSAPITRTHLVRYAGASGDFNPLHHDE
                     TFAQAVGLETVIAQGMFIMGIAGQAVTSWIDHRWLRKFSVHFLSMTQPVDLNDYQTTK
                     DRATITVSAKVVDKYEEDGEKKIRCELTARDAQGSRKLKGFFVAALP"
     gene            complement(23634..24110)
                     /locus_tag="PLA58_01750"
     CDS             complement(23634..24110)
                     /locus_tag="PLA58_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOR61549.1"
                     /translation="MADPSKLGMEFPVYSIRVEKNKIAEFVAALEQKDDTEQINPLYR
                     DVDAAKKAGFENIPTPPTFSTSYVFWTGGGLLGTIKAVGADLSKLLHTKEEYEYFAPV
                     CAGDVITRKMKVVDMYERGKKEQANRYMQVTVLETDLINQRGELVIRARTTFMERG"
     gene            24279..24599
                     /locus_tag="PLA58_01755"
     CDS             24279..24599
                     /locus_tag="PLA58_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61550.1"
                     /translation="MTKRIRRLYFLRAMKNCAHCQKEIPDERWIGRQAQCPFCGHDLR
                     ACLNCVFYEPGAFHDCRESQAERVLEKSRSNFCDYFQYRTGAEKPGRRPADVQEKLAA
                     LFKK"
     gene            complement(24618..25868)
                     /locus_tag="PLA58_01760"
     CDS             complement(24618..25868)
                     /locus_tag="PLA58_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOR61551.1"
                     /translation="MNAWKSWMMTVVLIFFLCGAASAAVRISPLPPPPPTAKLRVFVV
                     AVTTEFKSAKGPAFWPVSPEEFDRKQKQAIDERLKQQGIYEVVGESDIRAVLGTQKPA
                     GWEWLADDCRLVREAGRALHADYALVSERSFTIHLQFDTRLMNLYSGKEFVAEGYVPS
                     TMLRAMNKEKKTLAGAEAIKIQFRQIFQEAKGDLMRTAIRKGKIAAKGSQQVEKTVPP
                     PPEEKAAAGSEPLEKGPQAAQASKDKAPASSLPKTPGALAAGDQQRVFEKELEQALNP
                     KSPKKSGPRLIVYDFDAAEPMKVAGLILTEALREELCHFGDYVLIQRENMAKILEEYK
                     LQRSGLVDEKQAVRVGKWLAANEAVTGNLAVLGNISLLQAKRMDIETLGTLAAGSIKC
                     PAGREDELLDRMPELAAKLISGMK"
     gene            complement(25928..26419)
                     /locus_tag="PLA58_01765"
     CDS             complement(25928..26419)
                     /locus_tag="PLA58_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOR61552.1"
                     /translation="MPIYEFYCRKCNTIYNFFSRSVNTDKIPSCPRCKKVKLERRMSL
                     FSAITGKAKDGEEADPLRGMDEGKMEKAMMQLAAEAEGIREDDPRAAANLMRKLSDAT
                     GMKLGDGFQEALRRLEAGEDPDKIDEEMGDLLSAEDPFGENRRGRKSAARKPRKDETL
                     YDL"
     gene            complement(26501..27376)
                     /locus_tag="PLA58_01770"
     CDS             complement(26501..27376)
                     /locus_tag="PLA58_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HOR61553.1"
                     /translation="MSEPKRKSALVVEGGGMRGIFAAGVLRAFASAGFDPFDLYIGVS
                     AGACHLASHLAGQYDRNLAVTLRYSLSPRFINPWRFLRGGHLMDLDWMWEQTIRHDRL
                     DLTALFETLSRCNKEYRIVATSMETGKALYLSPGPDTLEHFLKVSSAIPVLYRKVLEV
                     GGEKATDGGVVDAIPAREAHRRGATDITVIRSRPVGYVKKESPVALMVLARYYRKYPG
                     LMKSFRRRADVYNAAVRFVRRPPPGVRVTEIAPPAELDVGRTTRDEARLRAAYSTGMD
                     CGSRYLRERAAGHSE"
     gene            complement(27469..27972)
                     /locus_tag="PLA58_01775"
     CDS             complement(27469..27972)
                     /locus_tag="PLA58_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61554.1"
                     /translation="MTLEEQIAELTRAGSGTLWELAGPGFMLLAAALALAGAFTRNPV
                     FFGIAPAAGLLAVAVRQTAPHIANAVRGLREGVKQPGAVEISFHSWKDAESNVHESCR
                     GLISMDGRPLWEMEFVTPEHWQPREGNYPVQLVFIRGVEWPVVVLTQEGLLYPRLKPR
                     KRQGQLQ"
     gene            28148..28417
                     /locus_tag="PLA58_01780"
     CDS             28148..28417
                     /locus_tag="PLA58_01780"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOR61555.1"
                     /translation="MFGIGVQELIIIAIIALLIVGPKKLPDLAKTLGKGFRDFKNATE
                     GVTEDLKDALKEDDKPKPDDGLKDSLLSKKPEAEEEKNKAPGNET"
     gene            28466..29242
                     /gene="tatC"
                     /locus_tag="PLA58_01785"
     CDS             28466..29242
                     /gene="tatC"
                     /locus_tag="PLA58_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOR61556.1"
                     /translation="METNEDLKMSLTEHLIELRKRLTNSLIALGIGFLACYAFKDYLF
                     AVITKPLTDALPKSSYLIFTGLTQAFFTYMKIAIFSSLVLTSPFIFYQVWKFIAPALL
                     PEEKKYVAPFVLSSTLLFAGGVLFGYFVVLPPAFEFFVSFNNEYLRAMISFNDYLSLF
                     IKFLLGFGLSFQLPVLVFFLAKLGIVNDKLLSRYRKYAILLMFIAAAILTPSPDALSQ
                     ILMAIPLLFLYEVSIFVAKFAARKKEAPPQPEAPGAEEEV"
     gene            29245..31275
                     /locus_tag="PLA58_01790"
     CDS             29245..31275
                     /locus_tag="PLA58_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOR61557.1"
                     /translation="MSPDKDRNGRRKTGSKRSPAIKITLAVFLVLFLAAAGGAVTYRV
                     MTKDLPGIDALKDYRPSIASRVLDENDMLIDEFFLEDRKMINIADVPKIVQYAFVAAE
                     DSRFYKHRGVDLLSIFRALFKNVEAGKIVQGGSTITQQVAKLMYLTPERKYVRKLKEA
                     ILSYRIDKYLTKDEILHLYLNQIYLGHGTYGIESASIGYFGKSARDLTLPEAALLAGL
                     PKAPTTYSPFLNFNRAKQRQIYVLTRMQEEGFISKDQMTQAAAAPLSLRPMKPKDKVA
                     AYFVEHVRRYVQEKYGADVLYKEGLTIYTTLNLSAQTAARDALMRGLSEMEARNKYAK
                     GLVQGALYCMDVKTGAIRALVGGRDFTKSEFNRAVQSRRQPGSAFKPLIYTAAFDKGM
                     NPSTRFVDSPIIMEDPSQPDGLWKPKNFDEKFLGPITMRTALVQSRNVVTVKILQEIG
                     VDYAISYAANLGIESPLPANLSLALGSAGVTLQEMVRAYGALANGGKKVSPYFIRKIV
                     DRTGNVFEETKPLSEQVIDPRIAFMTTYIMQDVVESGTGRRVKSIGRPVAGKTGTTND
                     VRDAWFIGFTPSLITGVWVGFDQEKSLGSHEVGGRAAAPIWLYFMEKALQDKPAEAFN
                     APPGIVFIRVDAKTGRPAPGAGPGTIAECFLEDNPPGENGNEMFGEREELFR"
     gene            31606..33309
                     /gene="ilvB"
                     /locus_tag="PLA58_01795"
     CDS             31606..33309
                     /gene="ilvB"
                     /locus_tag="PLA58_01795"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOR61558.1"
                     /translation="MKMKGTEILLKALEEENVDIIFGYPGGAVLDIYDQLYKSNLRHI
                     LVRHEQGAVHAADGYARATGKVGVCLVTSGPGATNAVTGVATANMDSIPLVVLTGQVP
                     TGLIGNDAFQECDITGITRPCTKHNFLVRNIEDLAATIKEAFHIARSGRPGPVLVDLP
                     KNVMAAQTEYDPGNVRIRNHEVLYKPAPKKMANLLSMLQEAKKPLIMTGGGVILSRAA
                     QELTDLARKYRIPVTGTLMGLGSFPASDPLWLGMLGMHGTYYANMAVSHCDLLLAVGV
                     RFDDRVTGSIETFAPEAKIVHIDIDPSSINKNVTVDLPIVGDAQSALTDLLRFLEEHH
                     YEPDIASRQAWLSEIDEWRGKVPLTYCQNGEVIKPQFVIQTLHRLTGGDALITTEVGQ
                     NQMWTAQFFGFDRPNTFISSGGLGTMGFGLPAAIGVKCAFPDKQVVDIAGDGSIQMNI
                     QELATAAQYNIAVKVVLLNNGYLGMVRQWQELFYEKRYSQTDMTYAPDFVRLAEAFGV
                     VGLRATKPGEVEEVLRQGLAVHKPVLMDFRVSREECVYPMVHPGESISQMALGTREMI
                     N"
     gene            33346..33831
                     /gene="ilvN"
                     /locus_tag="PLA58_01800"
     CDS             33346..33831
                     /gene="ilvN"
                     /locus_tag="PLA58_01800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOR61559.1"
                     /translation="MEKKDHIISILVYNKPDVLARIAGTLGGKGYNIDSLCVNTTTQN
                     DISKIVMTTVGSQATINRIVNELQKLVDVISADDLTNVESIHREMVLVRLNLTPANTE
                     AVKKALETHKWKVLVSGDTFVMIEITGDKTQIDFALARLEPLGVTDITRTGLVALKLD
                     D"
     gene            33865..>34812
                     /gene="ilvC"
                     /locus_tag="PLA58_01805"
     CDS             33865..>34812
                     /gene="ilvC"
                     /locus_tag="PLA58_01805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOR61560.1"
                     /translation="MAKINFGGTIEDVVTSEEFTVKKAQDVLKQEVIAVLGYGVQGPA
                     QAFNMKDNGINVIIGQSPEDKPYWDKAIADGWIPGRTLFPIEEAVEKATIIQYLVSDA
                     AQMILWPKVKPHLKKGDALYFSHGFSIVYKEQTGVIPPDFVDVIMVAPKGSGTSVRRN
                     FLDGSGINSSFAVHQDATGRAMERTLALGIAIGSGFLFPTTFQMEVYSDLTGERGVLM
                     GALAGMMEAQYAELRRHGHTPSEAFNETVEELTQSLIRLVGENGMDWMYANCSATAQR
                     GALDWRHKFRKAVEPVFAELYRSVAAGRETEIVLRVNSAP"
BASE COUNT         7940 a         9758 c         9360 g         7554 t
ORIGIN      
        1 tggcctctac cacacccgaa cagccaatgc aagcactatt taacagatgg ttttccggcg
       61 atgcgatctg cgctgagcag ccgttactgg gctgaaatca ccacccgggc ctcgctcaga
      121 taatcattcc cgtgggccgt cgtctctttg atcaggcggg ccgcttcccc cctcagaacg
      181 atcatgtttt ctttcgtaat tttggacgcc gggataatca tcacattgtc tcccaggtgc
      241 gtgttgtttc gagcctgatc aatgctgtcg gcgtatcgca cgacgccatg agtccggaca
      301 accgccggct tcacactctt ggcggaatag atcgtaaccg gcgagtcgcc tacttcctcg
      361 acgatgatca cgggcagcag cacgcgctcg aaaatccgtc cctccagatt gaccaccatg
      421 ccggtaaccg gacgggtttt gtcatagaca atggtgtatt ctttgggtgg gggaggcgtc
      481 tggacaaccg gaatgtcctg cagccttttg ctcggaacga acggagcatc atccttctgc
      541 tccacattca atgcctgaac cagggtcgtg gttccgggac agttgctgat acacacgcgc
      601 atctccacgg ttgccagaag ggacccatcc ggctggctct catacttttc tttatggatt
      661 ctggcgccct tgacaactcc gtcaatttgc gtccggataa tgtcctgggt gaccatgaaa
      721 ttttcgacgg tggtcacgga atcgattttc acgcctttga tgatttccag aaggttgcgg
      781 taggcatccc cgatggcggc tcttctggac aagggccttg acaaagccat gttgcctttc
      841 gccgccccgc cgtatccgac ggcgctaaca tacccgtcca cccagtcgat tttgccgtca
      901 acatcggaat aggccggcga aataaaaccc agccccatca gcaaaagcgc catacagcct
      961 atggagaata ccttcttcag tgacatgata accatccctt ctttacgatc tgttttttaa
     1021 aacatcttcc ccgtctttgg caagtcaaaa aaacgctcca tcaccgggcg acacgaaaga
     1081 attctatcta ccggaaatcg ttccgtaaaa ctttaagctg atcgaagaaa ttctgccctc
     1141 ccgggtcggc cagcccgatc cctcggcgcc gacggcatga atcatcttcg gcttcgggtt
     1201 cagcgacatc aaatcgactt cataggtgtg ccaactcccc tgactcactt ttgtaccgtg
     1261 ccaggtctgg ctgttaccgg tgggattggc atagtagaag ccgtgccaaa acatccgatt
     1321 cgtctgtgat tccgttgggt tagccatact gcccaacccg ttgcgaacca cgccggcaac
     1381 atcggtgtac gtcaccatca ctgcaaccgg agcttctctg ccctgccagc cggttcccgt
     1441 cagattctgc tccacggctt taaccacagc cttcaggatc aatctctgcg cgccggaaac
     1501 atcagcgttg accgtttgca tgattcccat ccgggtattg ccgacggtat tgcgaaactt
     1561 aatcccgtcg gattcctggg ttacctcgcc cctgtccccc gtaggctgca tccagcgatg
     1621 gatcgtccat ccgctgaggc ttttgagcgt gccgttggtg agcagctctt tttcccccac
     1681 tgtcaccgcc gttccggttt taacgccctt gatttccacg aacccggcgc ccgatgaacc
     1741 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacaggtggc gtaaccgggg
     1861 gggtcacggg cggtgtcaca ggtggagtga caggtggagt gactggcggc gtcacaggag
     1921 gcacaaccgg gggagtgaca gggggttcaa ccggtggcat taccgggggg ataaccggtg
     1981 gcgtaaccgg cggttcaacc ggaggagtaa cagggggggt aaccggcggc gttgcgggct
     2041 gctgcgtcgg caagccggcc ccgcggatgg caaaggatgc cctgctctcc cgcgaagttc
     2101 cgccgctcgc atccacggcc tgggcaatca cggaatagct cccgggcggc aggctgaagt
     2161 cttttgacgt cccttcccag agctttccgg tggcggaaat ctgctgggcg tccacctgtt
     2221 ttccggatgc gtcaaaaacc cggaaagtga tctcccgcat gccgacggtg tgcgtcgcct
     2281 tggttttgaa ggtaacattg ccggtgcccg gattattgac ggtcacatca agaatgaccg
     2341 gcggcgcggc cagaaccgtc gcgacctccc gcaactgatc gatcatatcc tttttgccgc
     2401 ttttctccgt tccggccagg tgctccttgc cccacgaatc acggtgctct ctgggtggct
     2461 gggaataggc agtcgccgga atggcgacct ttcctgtcac gagattgctt ttttgcccgt
     2521 cttcatcctg aatctggata gtgtaaccga tcgactcctg agaaaccatg gggtcggttt
     2581 caagaacgac gttgacttcg ccaagatagc ctaaacgcgt ttttttatta tcgtaaggat
     2641 ctactttgat ttccttgact tctttgccgg cggccacccg gacagagaca atttccaccg
     2701 aagacttcca ctgcagattg atggaaatct gccggtccag gtactgcgcg tcgtgccgtg
     2761 taatttccgg tttcctggcc tcggcattca ggctgaacac cgccagacag acaaggatcg
     2821 ccaacaggca tgagggtatg ctctttttca taacgtgaac ctttcttcat ctcttcatcg
     2881 tgaagacagc gggttaatga ccgtttgcct tattccaggt ctttgggcga aacgagtttg
     2941 atgttgaagg actcgtcaac aagcgcaatc cgggtgttcc atgctttggg cttcatggcg
     3001 tccgacactt ccgcgtcgat taaagtgatg ctgattttct ggcggatgat tttgtccgtc
     3061 cggggatcga tgcgcgaaaa gatgacgccg gaatagttct ggcagtcttc gatggcgggc
     3121 caggccggaa aggcgatggt ggttttcttg cgcctcggat cggttgactt taattccaca
     3181 acccaggcgg caacggtgtc ccggcttttt gcatgggctc tggggcagga ccagatgaca
     3241 ttgcggggaa gcgattttcc cgatcccggt tcgatcagct gcagagaggc tttcgcaaaa
     3301 agcccctctt tggaaaactc aacggttttg tcgagttcca gcgttctgtt tccggattca
     3361 ctttttgcgt aggtctcgga gtcgatcatg tgaagtttgt aggccacgac aagcccttct
     3421 ccttcggtgc cccgccgtgc ctgcgtgccc gctccggctg tagctgtcac gctgccgatg
     3481 gccgtgcccg cgccgacatt gagtccggct ttattggatt tttcgtcggc aatgcggaaa
     3541 ttggccagcc gcagggcttc cgtgatgtag ggaagccccg gctcaaaggc gatgtcggct
     3601 atggagacgg gggtgatgat ttccaggccg gagagctttg ctttctcgac gctgccgccg
     3661 gaggcgccga tctctgccgc cggacccgca atggtctgtc cgccgagacc gacccccaga
     3721 ttgtaggcaa aatcctgctg aatcccgctt aacgattttt cttttttcac ctcgatctct
     3781 tccgcgacag ggtcttcaag cggcccgaac tgcttcgacc aggtgcaacc gatgtaggcg
     3841 cggtccaccg gtttcttggt ggtgaaatat tcttcctccg taatggatga catgcccttt
     3901 tgatgggtct tcatcgctgt gccgctggtc gaaacgcagg aggttaaaaa aaacaggacc
     3961 agcaacgatc cgacaacccc agcgacaacc atactctttt tattttttcc catggcgatt
     4021 tcctccgtcc tctcccgctg aattttcgtc aaacctatta actggtttta ctataaaaaa
     4081 aaggggcttc cgtgtcaatc aaatagtctg tatgtttgga gcgggaatgc gtcaggaagg
     4141 aatggagaaa atggagcggg cgaccgggct cgaaccggcg acgtccagct tgggaagctg
     4201 acattctacc gctgaattac gcccgctcac ggcaaaagtc tttgatgcaa cacgctcttt
     4261 ttgtcaagga gtttttgcgc atgaccgatc atcgggcgat tgactttgcg gtcttatcgg
     4321 ttgacagcgc cgggattatc cgttaagagg ctgcaccgtt cagggaagaa ggatataagg
     4381 ataaaggctg aagactgaag gctgaaggga attttgtggt gaacggttga aaacgttccc
     4441 ttcagagatg agagtatttt gagcaaacaa ctgcaaaaag aaattgaacg ccgccggacg
     4501 ttcgggatca tcagccatcc cgacgcggga aaaacaacgc ttacggaaaa gctgctgctt
     4561 ttcggcggcg ccatccagat ggcgggcgcc gtcaaggcgc ggaaagcgtc caaacacgcg
     4621 ctttccgact ggatgtccat cgagaaagaa cgcggcattt ccgtcacctc gtcggtcatg
     4681 aaattcgaat acgccggctg cgagatcaac ctgctggaca cgccggggca tcaggacttt
     4741 tccgaagata cctaccgggt gctcaccgcc gtggacagcg cgctgatggt gattgacggc
     4801 gccaaaggcg tcgaaacgca aacgcagaag ctcatggaag tctgccgtct gcgcaacaca
     4861 ccgatcatca ccttcatcaa caagctggat cgcgactgcc tggcgcccct ggacattctc
     4921 acggacattg aagacaaact gcagctggaa tgttcgccgc tctcctggcc catcggcatg
     4981 ggcaagcggt tccggggcgt ttacaacctt tacgatcaaa aacttcatct ttttacgccg
     5041 ggccagtcaa agcgaacgga cgaaggcgtc gtgatcagcg atttgtccga tggactgctt
     5101 gatgaactgc tggacagcca ggccgcggag ctccgcgaag aaatcgccct gctcaccggc
     5161 gcagccaatc cctttgaaat cagagaatac ctgaaggcgg gacagacgcc ggtttttttc
     5221 ggcagcgcca tcaataattt cggcgtcagg gaattgctcg acgcctttgt ggaaatcgcc
     5281 ccttctcccg ttgcccgtcc cacgaccacg aggcaggttg agccggacga agatgatttt
     5341 tccggatttg tttttaaaat ccaggccaac atggatccgg cgcaccgcga ccgcattgcg
     5401 tttatgagaa tctgttcggg caagttcgag cggggcatgc gcgtggttca tcaccgcatt
     5461 cacaaggaga tctccctgtc caatgccacg attttcatgg cgcaggaccg gaccaatgtc
     5521 gaaacggctt acccgggaga catcatcggc atccacaatc acggcaccat caaagtgggc
     5581 gacaccttca cgcaaaaaga accgctgaag tttacaggca ttccccattt cgctccggag
     5641 cacttttgcc gcgtgcggct caaagatcct ctgcgcctca aacatctgca aaaggggctg
     5701 atgcagcttt cggaagaagg agcgattcag gtcttcaaac ctctctgggg ttcggacaac
     5761 attctgggcg cggtgggcgc tttgcagttt gacgtgacca tggcgcgtct gaaagacgaa
     5821 tacagcgttt atgccgatta cgaacaagcc gattacgccg ccgcccgctg ggtcacctgc
     5881 gacgacgccg gaaaaatgga agatttccag aagaagaatt ccatgaacct ggccacggac
     5941 acggaaggaa acctcgtata tctggcgctt tccgactggc gcctttccca cgtcatggaa
     6001 gagtggccgc aaatccggtt tcacaaaacc atggaaaaaa gctgagcaat gtgttttccg
     6061 ctgtccaaca ttagagacgg cggtagactg ttgacctgat tttattttat cttgaaccgt
     6121 taacgtcaat cggcaaacaa ggcttcaaaa tttcgcggat taaggatgac gataccctta
     6181 tatcttttga ggatcaacag gtcttcatca ccggtaacaa tatagtcggc ctgtgcatca
     6241 acggcacagg cgataatgat attatcctcc ggatccctgc aaatactcgg gacaggactc
     6301 agatcatgaa tgatttctga ggctgcttct gaaacaatgg ccgagatttc ggaaacatcg
     6361 gccggggtga ttttgaattt ttttctcagg ataccttcaa attcgccgat aatatcatcg
     6421 cacagaacga ggctgaatgc ctgcttgcgc gcgcggatca tcaggcctga acaaagccct
     6481 tctgtcagaa aggcggcaat cagaacattg gtgtcaaata ccgccttcac gatataacct
     6541 tgaaaacatc gtcatccgta atcaagcctg acgctttcgc tttagggccc agggttttct
     6601 tcatccggcg gaatctggcc tcccataaaa acacccccag cgactctttc acgatatcgc
     6661 ttttatttct tccggttgca cgggcaatgg catccagctc cgacgccatt ttctcgggca
     6721 aactgacaga cagaacgctt ctcatcatgc gcctcctgca ctacaataca ctacagcaca
     6781 acgtgagtca agtcaaaatt gattctcatg atcctccctt atgcctcttt gtccctctgt
     6841 tgccgttcag ggcctgtcgg gaaacagatc ggagcctgcc gccttcagcg ccggttcatc
     6901 attcaggatg gattcgacat acgcgaagaa ttttggaaat tccgcggcga tgaaaaatcg
     6961 cgacgtcatg atcttgttgt aatagaaaca tgcctccgaa tcgttccgga tattttcgaa
     7021 tgaacaatcc gcaacggcac ggccggttgt ctttttcagt tgggaagccg ccagagagag
     7081 gttccacaaa tgcatccatc cgaggcaaag aaggaacatc gccttttgga aaggcgtcgc
     7141 ctgggcaaag atgccggcga tctttccctg gcccatccgg gctttcagga atccgaccat
     7201 cttgtccaat tcgacaagtc ccctggccac aggcgtgata tacacctcct cgacggcctc
     7261 acgggccgtg ttcaccgttt gtttggtcag tttccggaaa acattcagat tccgttcatc
     7321 cggatccatc agaatcttcc tcatcaacaa atcgacagac tgaatgccgt ttgtcccctc
     7381 gacgatgctc agaattttac agtcacgcgc atactgctcc acgggatagt cccgacaata
     7441 gccgtaaccg ccgtaaacct ggattgcttc ggcggtcaca agccaggacg cgtcggtatt
     7501 acccgccttg cacagcggaa tcagcaattc ggtgagcgcc tttgcttcac ggcccttttc
     7561 tccctgatcg acagcctcca catcaagatt tttcccgagg aaataggtaa gcatccgcat
     7621 cccctcaacg taggatttca tccataggag catcctgacc acatcgggat gttcaatgat
     7681 gggaacttgc ggggcattcg gatccagcat ccccgtcacg tgaacgccct ggagccgggt
     7741 tccggcatag gcaatggcat gcatacacgc ggcagtggag caggaaagtc cctgaaggcc
     7801 tacaaaaagt ctttcttcgt tcatcatctt aaacatctgc cgcatccctt ttctttcctg
     7861 acccaggaga tagccgacgc aggagttgtt ttctccaaag gccagggcgc aggtggagag
     7921 tccgtgaatg cccatctttt cctcaatccc cgtgcagaca acatcgtttc gtctgccggg
     7981 tgttccatcc ggttcggaga tgaatttggg cacaataaag agggaaagcc cccgtgtgcc
     8041 gggcggatcg ccttcgatgc gcgccaggac catatgaaca atattgtccg ttaaatcgtg
     8101 gtcccctccg ctgatgaata ttttctgccc tgtgatttca taggcgccgt cttcacgacg
     8161 aaccgccttt gtcttgacat tgccgacatc ggagcctgca ttgggttcgg tcaggcacat
     8221 cgtgccgctc cattttccgg aaagcatttt cggcagatag gtttgcttct gttctttgct
     8281 tccgaaagaa taaacgagct gcgcggcact gccgctcaac agcgtgtaaa acgtcaggca
     8341 gccgttggcg gcgcaaaagt attctttgca agccaccgac acagacaaag gcaggcctaa
     8401 tccgccaaat gccggatcgg cggaaacccc gatgaatccc gcttcgctga tggcccggta
     8461 gggttgctgg aagcaggcgg gtaccgtcac cactccaagc ttattgtcaa agtggacacc
     8521 cacgcggtcc ccttcgctgt tgacgggata gaattgttcg acggatattt gttcagccag
     8581 acggagggta tcctcataca aggcttgatc ctggtcggaa aacttttcgt actgtaagca
     8641 ggagtccacc tccagcattt cgaacaggac gtaacggaca tcccgggaat caaccagtaa
     8701 attggacata acctctcctt caagatttaa gatggccgtg tcgcagcagg ctaagcggcc
     8761 tatgacagtt ttgcttcttt ccgccgggcg tcgatgacct ccagcagcgc ttcgatgcgc
     8821 gtgaaagatt gttcttcgct gtatttgcgc atatcggcat gatcaacatc gatcatcacc
     8881 gccggaatgc ccagttcatt cgtgacgcgt ttgagcatgt ccatttgcat gatggaatag
     8941 actttacagc ttctgttgct ggcgaagatg acaccgtcta cgccccagtc ttcaatggcg
     9001 cgccgcatcg cccgaaagcg aagcgacatt ccatgcgggc agatggaaga attctgcatt
     9061 cgcgccagat agaccagcgg atcaccgccg tcataggcaa acagatcaag ctcgccctcc
     9121 acggtcccga tgcaataggt gtaggaagct gaaatcaggt tgacgtcaag cgaagccagg
     9181 cgggacgaca tcctgctcaa ttgatgccag ggcgcaatgt tgtcccagag aagccggtat
     9241 ctttcattgg gaacaggata gatccccttc gcgatattat cttcggcctc ttcggcaaga
     9301 aggcggtagt gatcggccag ggtctttgtt ccccgggaag tgaggtacgg cgccatctgg
     9361 acaaaggaat cgaaggcggt aagaccggcc ggccgggacg ctgccgtttt caaaaagcgc
     9421 ttccagttgg ccagagcatc ccgcgtgttt tcgagcgcct cgcggactcg ctcgatattg
     9481 aatgtctgcc cgctcagatt ttcgatggtc cggatgaggt cgcggtactg ggtaacgatg
     9541 taatcgagat cccgcgcgtt ttgcggttca tagcagaacg gcacatccag aataaaatgc
     9601 ggaacacccc atgtccggtg gtagatgtcg aaccacttga ccagcaggga acagttgttg
     9661 ttgttggaga cgataagatg cggtttcggc aggcccatcg tgggagagtc cttcccgccg
     9721 tcaaacgccg tgccgagatc gatgcgtgca taagagcaca gatccattga atagcccatc
     9781 ttttccgctt tttcgcattg cagggggccg acgccttttc ccgcgctcat ggcggcatgg
     9841 ctctccggta cgccgtaaac cacgttttca aaaccgctga acaattcagc gggaacgacg
     9901 atggccatcc acaccacgaa ggcgccttca ccggcttttt gatgcagatt catgtagtca
     9961 ttgaacatga ttttactcaa tcgcctgccg gccgcagtct tctgatggcc ggattttgcc
    10021 gtattttcgt ttgccataaa aacctcccta aaccgtggaa tgcatcaatt cggcaaatgt
    10081 ctcgatacgc gttgtataag ccgcggtatt ttcatcgtca tcgagtgaaa tgtcgagcaa
    10141 gagggcccgt atccccatct ctttcagtat ctgctccaga taggggaact cgaagtattc
    10201 atactcgcag aatttctctc cgacaaagac aacgccccgg gcgccggtgt ccgaaacaag
    10261 tttacggatg gcgggcaagc gccggtccgc tgaatgcagc agcgtggtgc acggataatg
    10321 gtggtgataa aaatcgatgt agtattccgc cggatcgtca actgccggcg ggctataatg
    10381 aagcgcgcga ttttgtgagg cgacatcgtt gccaaccacc gtgatgcccg cgttttcaag
    10441 atcgcggatc atgggcagcg gcggaggcag gatgccgctg acgacaacgc ggggcgattc
    10501 cggggatggc ttggtttttt tccggaagtt gtcccggagg gcactctgca ggacaagcaa
    10561 ctggtcttcc acgggaataa accagccctc ggtgacaatc cgggagaact cgccataact
    10621 cattgaaccg tcggctacgc tggcgaagag cattttggac aactctctca tttcccggta
    10681 aagagatatg cttcggcgaa atttatccgg ggcgaaagtt acattgcagg tcttttccag
    10741 ttcgccgatt aatccctcga tgcttgcccg gagatagggc cggctgtcgg tctgatccgg
    10801 cgggactgcg gggatatgca ttttaaaaat gggaagcggc ggccttcctg cgtccgcaag
    10861 tccgcaatca ataatctccg gaaggttgcg caatgtgtcg caggcattat acataaacaa
    10921 tccgtcgagc gccgaactgt tttccttcag gagaaattcc atcagacggc gggcaaccga
    10981 gcaggcgtag ggctggatgt gccgatcgct ttctgcaacc cggggaaggg aatccctgag
    11041 cccccaaagg gtaacgggcg ccagacccat ggagtgaaaa agctccagag gagggtaaag
    11101 aggaaagcat ccgataacct tccgcccctg gtctttcaat ctttttatgt tgtccataat
    11161 atttttcata ggcgacctgt cttgttttaa tttgtttatg gccatcgtcg aacacatttc
    11221 ctgcatcata gatttagctg acttgaaccg tgttgtccga ccagtatttt ttccggatat
    11281 cctttttcaa aatctttccg gtcagcgtcc gcggaatggc atcgacgaag tcaaccgatt
    11341 tcgggatttt gacttttgac agattgtcct tgcaaaaccc gataacctct tcctcgctta
    11401 aatccgcatt ttttacggga acgacaacgg ccttaaccgc ctccccccat ttctcatccg
    11461 ggacgccgat taccgccaca tcttgaatat ttggcatccg atgcaggact tcctcgattt
    11521 ccaccggata aacattcaca ccgccggtga tgatcatatc gttcaggcga tccacaatat
    11581 aatagtaacc ttcttcatcc tgacgggcaa tgtctccgac cgtggcccat ccgccttcca
    11641 aaaaagcttt gtttgtggcg gcttcatttt cccagtagcc gcggaaacct cccaggccgc
    11701 gtgaatagag gattcccggc tcgccctgcc ggacttccat gccctcttca ttgaacaact
    11761 tcaattcgag gtcatgtccg ggcttgccga cggaacgttc ctttcgcagt tgatcctcat
    11821 cccgcagaca ggtggatacc ccgagttccg tgctgccgta aaattcattg agttccgcgt
    11881 ttttgaaggt ttccttgatt tttatctttg tgggtgtgtg cagcggcgct ccgccggaga
    11941 tgagtgttct taaagaactg acgtcgtatt tattcagaac ctcctgcggg agcagcatca
    12001 gccagtcgta catgaccgga accatcatca gccagtttac ccggtatttt tcgatattct
    12061 gcagaacttt ctccggaaca taggacggca tgatgacctg cgtgcctccg gaaatgagcg
    12121 tagccagaaa ccaggcgcag gcgcctgcgt gatattgggg aataacgacc atctggacat
    12181 cgcccatccg gattctccaa tcgatgcaaa cggtgacgcc ggtatgataa tcctccatga
    12241 cgccgcggac ggctcctttg ggaaaccccg tcgtgcctga cgtataaagc agcatatcaa
    12301 tgtcgtaggg ggatacttcc attcccctgt cttcgggcga agactcccgg atcaattgtt
    12361 cataggaaat gtgatccttc gtgacattac tgcccgtgac gatgacatgt tttaatgctg
    12421 gcacattggg ttttgcggca atcaacacat cgaggagttc gggagaggtg acaacggcgc
    12481 aggcgccgga atcattcaac tgatgggcca attcggcggc tttcagtcct gaactaaccg
    12541 gtgtcttaat gacgcctgag tgataggtcg atataaaggt ttcagaaaat tcaatgctgt
    12601 ttttcagata aatagccagg cgatccccct tggtcattcc taaatccatc catccgtttc
    12661 ccaatcgcgc cgtacggtcc cacatctgct cccatgaaac agtgcggtcc tcacagatta
    12721 atgccggcat ctcccgattt tttgcacaat tcattcgaat gctgctgatt acgttattcg
    12781 aataccgccc ttgaggtttg ctgaaattcg ctgtgatgtt catattgcct ccttgagaaa
    12841 gattttgttc aaacaagcaa tgaattgaac tacgtttatt tatctgaact ttgttcagtt
    12901 tgtcaacaaa tttaaataat attttatatt ccgtagtatt tacatgttgt tacaggaatt
    12961 cgcttgccaa aagaaatgaa atgccatatg gttcggctta aaaaatcatc tgccttcgaa
    13021 gcgaaaaatt tgcctatgaa agagaaaaaa acctttaata aattaaaaca acaggaatgg
    13081 gaggtccgac gcaatttgat catggatgcg gcacagcgcg ttttcgcctc cgaaaccttc
    13141 aacaaggcca acatgcggga aatcgcccgg gaggccaaca tttcaccggg ttcgatctac
    13201 aactacttcg agaatcaaga agaacttttt ttggcagctt ccctgcgggg agcggacaga
    13261 ctgattaaag tgtttagtga tttggttgcc gaggatcacc cctccattca gactgccgct
    13321 gtggcctata tcgactttat ctcaaaaaac tatgaatatt tgagaatggt tcaacacagc
    13381 atgctgtatg cccaatttac ctctcaggaa tcgattgatc aattggtcga gacggcccgg
    13441 aagttatttg aacaatttga tatgatcatt caacaataca tctcttccgg taacgtcagg
    13501 aaattttccc atctcttttt tgtcgctctg aacggaattt tattttcata cggcagattt
    13561 ccggaccgga caagcgatga ggtgcttacg tatatgaagg aacttgcatc ccttctggga
    13621 gaactgctgg agaaatatca gccgaagtaa cttttatgtc atgtcgcgtt tcggatttat
    13681 cggcgaagcg gcgggggaaa tacgatgaac caacagcctg tcaaaaaaaa ccggggcgaa
    13741 aaaaacacgg ggccgaatcc ggaagagcaa ggaagcgccg gaacgatcgg cggaagccag
    13801 aaacgcgctc gatccatgat gaagtccggg gtggccgcgg cttctcaagc ggaaagactt
    13861 cgggcgcggc aaaacctccg ggaaagtaaa aaacgctacc ggctgctggc ggaaaatctg
    13921 acggacgtca tctgggtgct ggatatgcgg ttgaagcacg tttatgtcag cccgtccgtg
    13981 gaaaatctgc gcggattcac tcccgaagag gccatgaaac agcctctgga agaggttcta
    14041 acgccggatt cctaccggaa ggtgctggac atcctctccc gggagctttc tctggaaacc
    14101 gggggacagc gacgccaacg gggatggact caaaatattg aattggaaat gctccgcaag
    14161 gacggttcta cgatttggac ggaagtcaaa atctcccttc tctatgatga acacaaccgg
    14221 ccgcaggaaa tcctcggcat tacccgcgac atcacggaac gaaagaaagc cgaggaagcg
    14281 ctgaaaaaga gcgaggagta ttaccgggcg atctttgaac acacggccac cgccaacatg
    14341 attctggctg aggacacgac cgttctgtcc gtgaacgcca atttcgaaaa aaccatggga
    14401 tattccaaga aaaacgttga aaacaaaatg tcctggactc aattcgtcgt tgacgaagac
    14461 gtggaagtca tgaaacaaag gcatgtgcaa aggagaatcc atcccgaatc cgttccctcc
    14521 acctatgaat tccggggact gacccaatcg ggagacatcc ggtctttctt cctcagcgtc
    14581 gatatgatcc ccggcacaac caacagcgtg gcctccctgt tcgacatcac cgaccgcaaa
    14641 aaagcggaag acgacctgcg gcaatccgaa gaacggtttc gggacatggc ccgcctgctg
    14701 ccggaaaccg tctttgaagc ggatttgaac ggaacgttca cctttgtcaa cgaaccgtct
    14761 tttgaccggt tcggtttcac ccgcaacgac atgaaaaaag gtctgtcgct cacggatgtt
    14821 ctggctccgg acgaccggga gcgtgcgcgg gcgaattttc aaaaaatcat ccggggagaa
    14881 tcttcagggc tcaacgaata cgtcgcacag aaaaaagaca agacgcggtt tccggtcatg
    14941 gtgcatacct cccccatttt caaagaaggg aaaacggtgg ggtttcgggg atttctcatc
    15001 gatatttcgg agaagaaggc tatggagagc cagcttttgc gggcgcagaa gctggaggcc
    15061 atcggaacac tggccggcgg cattgcccac gatttcaaca atctgctgat gggcatcctc
    15121 ggaaatattt ccctgatgct catgcatatc gatcaggggc accccttcca tgaacgcctc
    15181 aagaacatgg aagaatatgt ccagaaaggc tccgatctga cccggcagct tctgggcttc
    15241 gcccggggcg gcaaatacga agtccggacg accaaccttg gcaaattcgc cgcccgcagc
    15301 gcggaaatgt tcggcagaac cagaaaagaa atatccatcc accataccgc ggaaaaagac
    15361 ctctggcatg tggacgtgga ccgaggccag atggatcagg tgctgctcaa tcttttcgtg
    15421 aatgcgtggc aggcaatgcc cggcggagga aatctttata ttgcggtgga aaataccgaa
    15481 ctgggcgccc aggatgtggc cggtttcggc gtcacgccgg gcagatttgt caagctgacg
    15541 gtgagggaca cgggagtcgg aatggatgaa tccacgcgat cccgcatttt cgagccattt
    15601 ttttccacca aggagcgggg gcggggcacg gggctcggcc tggcctctgt ttacggaatc
    15661 atcaaaaatc acggcggatt catcggcgtg gaaagcgaaa taggtgtcgg cagcaccttt
    15721 accatccatc ttccggcgtc ggaggaaaaa accgaagagg aagatcaacc tgaaagtaag
    15781 cccgaccggg gccggggaac cgtcctgatc attgacgacg aagaaatgat tgttgatgtc
    15841 ggccggcaaa tgctggaagc gctgggatac attgtcctga ccgccggcgg cggcagggag
    15901 ggcattgaca tttttcaacg caatgccaag agcgtcgatc tggtcatcct ggacatgatc
    15961 atgccggatt taagcggcag agaaacgttt gaagcgcttc ggcgccggaa ccccgccgta
    16021 aacgtgctgc tctccagcgg ctacagtctg gacagccagg caaaagacat gatggccgaa
    16081 ggttgcaagg gctttatcca gaaacctttc acgatggccg aattatcccg gaagattcgg
    16141 gaaatgattg aaaaatgacg agctttttct tcgtgccgcc gccccatcgc ctatagccta
    16201 ttgcccattg cctatcgccc gtgtttgtaa tgttctaaaa ttttctcagg aacggttctt
    16261 tgcggcggca aaggccgcgg cgttttctcc ggccacatat ccggtggaga atgcggcttg
    16321 caggttatag ccgccggtgt cggcgtcgat gtccatcacc tcgccgcaaa aataaaagcc
    16381 ggggactttc ttcgacatca gcgttcgcgg atcaatgtcg tccagcgcaa cgccgcccgc
    16441 cgtcacaatg gctttggcca gcggcagcgg ggcctgaatg ttgaaccgca gcgccttgag
    16501 cacatcgatt attttctccc gctcagcggc gccgacctga ttcgccggct tgttttcgtc
    16561 cacgccggaa agagccgcaa gcgggccgac catcttggcc ggcagatagt ccttccagat
    16621 cgtcgctatt tttcttttgc cgaagtcatt gaaatccttt tgcagtcttt tgccgagctg
    16681 ctcccgggtc acggcgggtt ttaaatcaat caggatggaa accggccccc gctcaagggc
    16741 gtccaccacg gcgagactca aaagcagaac aatcggcccg cccaggccga aatgggtaaa
    16801 cagcatttcg ccgaaccggc tttcgatgag cggcggccgg ggttttttgc tttcaccgcg
    16861 gccatagtcg catggcggcg caagcgcggc gtctatcgtt cccgcttctc cctgaaacgc
    16921 cgtggcgcgg acattgcgca gagacacgcc ctgcatcgac cgggcaagcg cctgttcccg
    16981 gacgatcagc ggaaccagcg ccggcctgag tttgacgatg gtgtgcccca gcgcggcgct
    17041 caaggcatag ccgtcgcctt ctgagccggt agccggccag gaggcgccgc cggcggcaag
    17101 aatcaccgac gaagccaggt agcggccgcc cgttgtctca acgacaaaga gggaatccgg
    17161 acgaagcgcc acggaagtca cccgggcatg gagaagcctg cggacgcgat gctccgtcag
    17221 gtatttttga aaaacccgca ccacgtcgcg ggcgtcgtcg gaaacgggaa aaattctgcc
    17281 gccccgctcc actttggtcg cgaggccgta ccgttcaaaa aacgccagca gttcggaacg
    17341 gaaaaagcgt gaaaacgcgc cgtgcagaaa acggccgttg ggcccgtaca tggcaataaa
    17401 agattccagg tccgcggcgt tggtcagatt gcagcgggtt ttgccgctga ccagaatttt
    17461 tttgccgcac ccctccattt tttccaaaag cagcacccgg gcgcctcgct cggccgcccg
    17521 tccggcggcc atgagccccg ccgctccgcc gcccaccaca acaacatcgg cctccatcgt
    17581 cgtctgatcc atcccggcat ctccttggac aacacagcta tcacaaatgc aagaaaacgg
    17641 tcaatccatc tgcccggcct tgagaaccaa accgtcctgt gctaaagaat cttcggtcca
    17701 acttgcgtct caggagcatc atttgcgaac gccaacccat attctatctt tgaccaccag
    17761 aatgatcctg caggtccgcc cggcggcgca tgggttttta caccggtgga aagagcgcgc
    17821 acgccggatt cccgatcccg aattgcgccg tcaggctcta gcgagcattg aaaccaaaac
    17881 gttccactgc gaaggaggcg ctctctgcgg cctgctggcc ggaggcttct tccgggaggc
    17941 cattcaattt atcgtcgcct atcaaacgat cagcgattat ctggacaatc tgtgcgaccg
    18001 cagcacatcg caggatccgg aagattttcg cgcgctgcat gaagccatgc tgcatgccct
    18061 gacaccggaa gctccgactg ccgactatta ccggttccgg cgggagcacg atgacggcgg
    18121 ctatctggcc gaactggcgc ggacctgcca aacaattctg ggcggccagc cggaataccg
    18181 ccgggttgcg ccgtccatcc gggcgctggc ccgcctttac gcggatctgc aggtgcacaa
    18241 gcatgttcgc cccgatgagc gggaagcgcg cctgaagaag tggttcgaaa accacagggc
    18301 cggacttccg gaaatggcct ggcatgaatt tgccgccggt gccggctcga cgctgggaat
    18361 tttctgcatg gtctcgcagc tgatccggaa gcaggacccc ttaccggtcg tcgaacagat
    18421 tcacaaggcg tatttcccct gggtccaggg cctccacatt cttctggatt atctgatcga
    18481 ccaggaagag gaccggcgcg gcggagattt gaatttttgc tcttattatg aaaatcatga
    18541 acggctgacg ttccggctct gtcattttta cagcatggcg cacgcgagcg tggcggacct
    18601 gcccgacgca aaatttcatc acctgatgat cagcgggctt ttgagcatct atctgtccga
    18661 tcggaaggtt tcccgacaaa aagacgtgcg ggccattgcc aggaaactgc tcgctctggg
    18721 aggagctgaa acctttttct tttacctgca ctgctgggtt tatcgcaggc tgtcctgatt
    18781 tccgggggta ttttacccca tccattgggg cgttattccc ccttgggcag tccaatacgg
    18841 aaggagcagt cccaatttta aaaatttaac cgtcattaca aatgaataga ggttgacaga
    18901 tcggcacaaa aactggcatg gtcattgctc aaaaacaatt gcaaaaataa tcagggtacc
    18961 ggcattctat gaaaaaagct ttggtttgcg caaccgggat tgtctgcctt ctggcggtgt
    19021 tcgctttcgc cgccgaaaag cccagcgccc gctcgacgca aagcgttctt cccaaaacag
    19081 ccaccatgaa cgcccggggc aaagtggtgg aaatttccga aaaggccgtc cggattgaac
    19141 gaagcatcaa aggcaacgcg gaagtcatgg agtttgccct ggaaaacccg gccgccgata
    19201 ttgccgtgaa cgattttgtc aaaatcgatt acacgatcaa agacggcaaa ttgaccgcct
    19261 ccagggtggc caaaccaaag gcggaaaaaa attatagaaa aaatggatca aaacaggtaa
    19321 aggaaaaatc ggcacccgtt acaaagtaat gtagatgcct gtctatatat ggtatggagt
    19381 ccctgaatgt cgatgggttt atttaaaaac tattaaaaca aggaggatgt caaaatgaag
    19441 cgtttagtat tgtttgtgat cgtagccgtt ttcgcccttt gcaccgtggc catggcgcag
    19501 gtttccacaa aggatgaggt tcagtccgga ggaaccaaga tttccgacgc ggataaagcc
    19561 gccgtgaaag acgcgcagaa ggccgcgaaa gagaaaagag ccgcgaaaaa agcgggcttg
    19621 gtcaaaaaag aaaaagtgaa ggcgccggaa gccaaaaaag gcgtttccac ggttgaagaa
    19681 gttcagtccg gtggaaccaa gatttccgac gcggataaag ccgccgtgaa agatgcgcag
    19741 aaggccgcga aggaagaaag agccgcgaaa aaagcgggcc tggtcaaaaa agaaaaagtg
    19801 aaggcgccgg aagccaaaaa aggcgtttcc acggttgaag aagttcagtc cggtggaacc
    19861 aagatttccg acgcggataa agccgccgtg aaagatgcgc agaaggccgc gaaagaggca
    19921 aaagctgcga aaaaagccgc taagtaaaga agccaaataa gccacagcgg cagatgcgct
    19981 gcaatgttgg caaaaaaaag gacgggggaa tgaccgtcct ttttttatgc cgttttgggt
    20041 tccggatcca agcgggtgca ttaccccccc cgtggttaat tttcaaacaa tctgttataa
    20101 acgtatccca tgctgaaaaa actgctgtca aaaatcatcg attacaacaa caatctgacg
    20161 ctccgggtga ggctggttgc gctgaccgtg gccggcctgg cgctgaccat ggccgtctgg
    20221 gggctgattc acctgaccgc cctggaccgg attctggttg accaacaggg aaagcgcctg
    20281 gaaggcgtcg ccgaaaccgt cagcaccttt tatcagcact tcccgacccg gcagggcatc
    20341 gccacgcttg attccacatt gaaagatcat atccagaccg atgtgcggct ggcccgcatc
    20401 gacattatgg aaatccggcg caacgccgtc aactacgttg ccggcgccag ccgcgtgccc
    20461 tatgagtggc cggatcatct cgtggctgaa accggcgcaa aaggcaaacc cctttccatg
    20521 aaactggaaa cggaaggagg accgtcgctg ggcctgcttt atcctgtttc cggagacacg
    20581 agggactccc gcattgtggt gggcatcatt gctttttcgc gggccaacgc ggaaattatg
    20641 agccgcgccc ggcagctcct gctggtcagc agcgcgggac ttctgctggc cattcttttg
    20701 ctgctcggcg taagctacgg ctggctgatc ggccggccgc tggaaatgat catcgaaacc
    20761 atcgatgaat ttcagaaagg gcgctacgtc gaacgcattc ccattgtccg ccgggacgaa
    20821 tggggacatc tggccgatca tttcaatcaa atggccgatg aaattcaaaa tgtcatggaa
    20881 cgaaatgaaa atctcacccg ccacctggcg gaccgcgtcc gggaagaaac gctcaacgtc
    20941 atccacctgc aaaagcaggt ggatgattta aagcaattga cggccctggg gcatttgacc
    21001 gccaatctgg cccatgatct gggaaccccg cttcattcca tcgccggact ggcccggctg
    21061 atgctcgagc gcgaaggctg gccgccggat gtggcgcata aattgaacgt gatcgtcgaa
    21121 cagacgcagc ggctggacca tgtcattcag aatgtccgca aggccacgcg tcttccggaa
    21181 cctcatctgg aattgatgac cgtccataaa attctgagtg aaacgctgcc gctggttgag
    21241 cctctgatcc aaaaaaaccg gatcaccatc gaggttcggc tggaacggtc cgttgcgtct
    21301 ttgtacgcgg accgctaccg gattcaaacc gcgcttctga atattctgca aaattccatc
    21361 gaagccatgc cgggcggcgg ggtcatccgc atctcggccc aggacgatcg ggcggcccga
    21421 acggtcgtca tcgccatttc cgacaacgga ccgggcattg aggcggatct cctgcaaaga
    21481 gtctgcgatc ccttcgtgag ctcgcattcg ggtgaaggca tgcgcggcct gggattggcc
    21541 attgtccgcg acatcatcaa ggcccacaac ggccgcatgg atattcagag cgtgccggga
    21601 agcggcacgt ccgtcatcct ttacctgcgg atggccgacg acgcaacgga gtgattttta
    21661 cttttcttcc tgcaggctgt agcggcggat ttttgtccgc agggttttgc ggtcaatccc
    21721 cagaatctcg gcggctctgg actgattcca ctccgtcttt ttcagggtct gaacaatctg
    21781 ttcgcgctcg gcttcttcca gaggcgtcca ttccggcggc cccgcctgcg cactcacgac
    21841 cgcccgggtt cggaaccggt ccggcagatt ttccggcaaa atgaccacga acggcgagag
    21901 cagcagcgtc tgccgcaaaa cattttccag ctcccgaaca ttgccgggcc agtcatagcc
    21961 catgagggtt tccatagcct ccggggaaac ccgcacatcg gggtagccca gttttttcag
    22021 catcgtctcg atcagcaagg gaatgtccga ccggcgctcc cgcagaggcg gaatgagcag
    22081 acgcacgatg aagcgataca aaaggtccag acggaaccgg cctgcggcga cctcttcgtc
    22141 aatattttta ttggcggccg ccacgacgcg caccgcgacg tggcgaacgt cgtggccgcc
    22201 gatcctccgg acctcgccgt cctgcatgaa ccggaggagc ttgctttgca gcgccgtgga
    22261 catttccgtg atctcatcga ggaatatggt gccgcccccg gcggattcga tcagccccac
    22321 atgctgatgg tccgcgccgg taaaggaccc tttctcgtag ccgaaaagct ccgattcgag
    22381 caggttgtcg gggatcgcgc cgcaatgcac gacgacaaaa ggcttctggc ggcgagagga
    22441 catggaatgc agcgaccggg cggtcaattc cttgccggtg ccgctttcgc cctcaataag
    22501 cacgctggcc ggcgaatcgg cgacgcgggc aatnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22621 nnnnnnnnnn nnntgccgct ttcgccctca ataagcacgc tggccggcga atcggcgacg
    22681 cgggcaatct gtttgtagaa ctccaccatc acgggcgaag agccgatcag cttggcttct
    22741 tctgcggctt ccaggggctc actcgaccgg tgctttcgaa gctcgcgcac ctccagcact
    22801 ttctttacca gcgcgcggca gtcctccggt gaaaaaggtt tactgatgta atcccacgcc
    22861 cccagacgga ttgcctccat ggtggtttcc agagaaccgt aagcggtcat caggatgacg
    22921 ggcaggttcg gccattgttt ttgtatttcg tccaaaagct gcaggccgtc cattccgggc
    22981 atgcgaatgt cggacatcag caaatcatag tcggcgaggt tctgcttcag ggcggccgac
    23041 gcggaggtaa agctgctgac gtcataaccg tcgcgctgga gcacttcctg gagaaattca
    23101 caggcaacgg catcgtcatc aatcactatt atgcgggctt tcataaaaac acctgcggcc
    23161 attaatgagg ttgaaggatc agggcagggc cgccacgaaa aaacctttga gctttctgct
    23221 gccctgcgca tcccgggccg tcagctcaca gcggattttt ttctcgccgt cttcttcata
    23281 tttgtccacg actttagcgc tcaccgtaat cgtcgcccgg tctttggtgg tctgataatc
    23341 gttcagatcg accggctggg tcatgctgag gaaatgcacg gaaaattttc ttaaccatcg
    23401 atggtcgatc caggaagtga cggcctggcc cgcgatcccc ataataaaca tgccctgggc
    23461 aatgaccgtc tccagcccta ccgcctgggc aaaggtttca tcgtgatgca ggggattgaa
    23521 atcaccggag gcgccggcat agcgcaccag atgcgtccgc gtgatggggg cggaggtgta
    23581 aggcggcatg acgtccccga tttgaatttc gtcaaaaacc agcgccatgc cgttcagcct
    23641 ctttccatga aggtcgtgcg cgccctgatg accagctctc cgcgctgatt gatcaaatcc
    23701 gtttccagaa cggtcacctg catataccgg ttggcctgct ctttctttcc tctttcatac
    23761 atgtccacta ctttcatttt gcgggtgatg acatcgcccg cgcagaccgg cgcaaaatac
    23821 tcatactctt ccttggtatg cagcagcttg ctcaaatcgg cccccacggc tttgatcgtg
    23881 ccgagaagac cgccgccggt ccagaagaca tagctggtgg agaaagtggg aggcgtggga
    23941 atgttttcaa atccggcttt tttcgcggca tcgacatccc ggtaaagagg gttgatctgt
    24001 tccgtgtcgt ccttctgctc gagcgccgcg acaaattccg cgatcttgtt tttttccacc
    24061 cggatggaat aaacgggaaa ctccatgccg agttttgacg ggtcggccat agcaacctcc
    24121 ggaatatggt caagaaatca tgagagtcct atcacattca aaacggaatt tcacgttgaa
    24181 atgaccaaaa gagctgccgg aatggattta tcgacgccca ttgcctcatc aatacatgta
    24241 acccgaaaaa gagcgttcga acaacactcg aaagcctctt gacaaaaagg atacgccgcc
    24301 tgtatttttt gcgcgccatg aaaaattgcg ctcactgtca aaaagaaatt cccgacgaac
    24361 gatggatcgg ccggcaggcg cagtgcccct tttgcggcca cgacctgcgc gcatgcctga
    24421 actgcgtctt ttatgagccg ggggccttcc atgactgccg ggaaagccag gcggagcggg
    24481 tcctggagaa aagccgctcc aatttctgcg actactttca atacaggacc ggcgcggaga
    24541 aaccgggcag gcggccggcc gacgttcagg aaaagctggc cgccctgttc aaaaaataat
    24601 cgccgggcgg ccgtctgtta tttcatcccg gagatcagtt ttgcggccag ctccggcatc
    24661 cggtcgagca gttcgtcttc gcggccggcc ggacatttga tcgaacccgc ggccagcgtg
    24721 cccagggttt cgatatccat ccgtttggcc tgcaggaggc tgatgtttcc cagcacggcc
    24781 agatttcccg tcaccgcctc attggccgcc agccatttgc cgacccggac ggcctgtttt
    24841 tcatccacca gaccggagcg ctgcaattta tactcctcca ggattttcgc catgttttcc
    24901 cgctggatga gcacgtagtc gccaaaatgg cataattctt cacgcagcgc ctccgtcaga
    24961 atcaatcccg caaccttcat cggctccgcg gcgtcaaaat cataaacgat cagacgggga
    25021 ccgctctttt tgggactttt ggggttgagc gcctgttcca gctccttttc aaacactctt
    25081 tgctgatcac ccgcggcaag ggccccgggc gtctttggaa gcgaagaggc cggggccttg
    25141 tcttttgaag cctgcgcggc ctgcggtcct ttttccaggg gctccgatcc ggcggctgcc
    25201 ttctcctccg gaggcggcgg gaccgtcttt tcgacctgct gagatccttt cgccgcgatt
    25261 tttcctttgc ggatggccgt tctcatcaga tcgcccttgg cttcctgaaa gatctggcgg
    25321 aattgaattt ttatagcttc ggcgcccgca agcgtttttt tctctttgtt catcgcccgc
    25381 agcatcgtgg acggaacgta accttccgcg acaaactctt ttcccgaata taaattcatc
    25441 aaccgcgtgt cgaattggag gtggatggtg aaactgcgct cggaaaccag cgcgtagtcg
    25501 gcatggaggg cgcggcccgc ctcacggacc agccggcagt catcggccag ccactcccag
    25561 cccgccggct tttgggtccc gagaacagca cggatatcgc tctcgccgac cacctcgtaa
    25621 atgccctgct gtttcagccg ttcatcaatc gcctgctttt gtttccgatc gaattcttcc
    25681 ggcgaaaccg gccagaaggc aggacctttc gcggatttga attccgtcgt caccgcgacg
    25741 acaaagaccc gaagcttcgc cgtaggaggc ggcgggggca gcggtgaaat ccggaccgcc
    25801 gcggacgcgg cgccgcatag aaagaaaatc agaacaaccg tcatcatcca ggacttccaa
    25861 gcgttcatgt tcatcccccc ctgcacgttt cggatctgaa cgggttgttg cccaagccac
    25921 agcccgttca gaggtcatac aatgtttcat ccttgcgggg tttccgggcg gcggacttcc
    25981 tgcctctcct gttttctccg aacggatctt ccgccgacag aaggtccccc atttcctcgt
    26041 cgattttgtc gggatcctcg cccgcctcca gacgccgcag ggcttcctga aaaccgtccc
    26101 ccagtttcat cccggtggca tccgataact tgcgcatcag gttggccgcg gcgcgcgggt
    26161 cgtcctcccg gatgccttcg gcttccgccg cgagctgcat catggctttt tccatcttgc
    26221 cctcatccat gccgcgcaga ggatccgctt cttccccgtc tttggccttt ccggtgatgg
    26281 ccgaaaaaag ggacattcgg cgctcaagct tcaccttttt acacctggga caggagggga
    26341 ttttgtcggt attgaccgat cgggaaaaga aattatagat ggtgttgcat ttccggcaat
    26401 aaaattcata aatcggcata tttatagctc ccggacgttt gatgctttta taaaacattg
    26461 tccgcaaata ttcaaccgtt tcggcgcccc gaaccatccc tcattctgaa tgcccggcgg
    26521 cgcgttcccg cagataccgg ctgccgcagt ccatgccggt ggaataggcg gcgcgcagac
    26581 gggcttcgtc ccgggtggtc cggccgacat ccaactccgc cggtggagcg atttcggtca
    26641 cccggacgcc cggaggagga cggcggacaa aacgcacggc ggcattatag acatcggcgc
    26701 ggcggcgaaa ggatttcatc aacccggggt atttccggta ataccgggca agcaccatga
    26761 gcgcaaccgg actttccttt tttacgtaac ccaccggccg ggaccggata acggtaatgt
    26821 cggttgcccc tcgccggtgc gcttcgcgcg ccggaatggc atccaccacg ccgccatcgg
    26881 tcgccttctc gcctccgacc tccagaacct tgcggtaaag aaccggaata gcgctggaaa
    26941 ctttgaggaa atgctcgagc gtgtccggcc cggggctcag atacagcgcc ttgccggttt
    27001 ccatggaggt ggccacaatc cgatattcct tgttgcagcg ggagagtgtt tcaaaaagag
    27061 cggtcaggtc cagccggtca tgacggatgg tctgctccca catccagtcc agatccatca
    27121 ggtgcccgcc gcgaagaaac cgccaggggt tgatgaaccg gggggacagg gaataacgca
    27181 gggtaaccgc caggttgcgg tcgtactgcc ccgccagatg tgaagccaga tgacaggcgc
    27241 ctgcggacac accgatatac aaatcaaacg gatcgaaccc ggcgctcgcg aatgcgcgca
    27301 gaactcccgc ggcaaaaatg ccgcgcatgc ccccaccttc caccaccagc gctgattttc
    27361 ttttcggttc gctcatcact gaaaaacgcc ctgttgtcaa aacgtcgcag catccgcggg
    27421 aacattcgat ccggaagcga aaagtcccgg ccggttcccg tcacttcatt attgtagctg
    27481 tccctgcctt ttccggggtt tcaaccgggg atacagcaag ccttcctgcg tcagaacgac
    27541 gaccggccat tccacacccc gaataaagac cagttggacc gggtaattgc cttcccgggg
    27601 ctgccagtgc tcgggcgtca cgaactccat ctcccacaga ggccggccgt ccatggagat
    27661 cagcccccgg caggactcgt gaacgtttga ttcggcgtcc ttccatgaat ggaaagagat
    27721 ttcgacggcg ccgggctgct tcacgccttc gcgcaaacct cggacggcgt tcgcgatatg
    27781 cggggcggtc tgccggacgg caaccgcgag aagcccggcc gccggcgcaa tcccgaaaaa
    27841 caccgggttg cgggtaaaag cgccggccag cgcaagggcc gcggccagca acatgaaacc
    27901 ggggcccgcc aattcccaca gggtgcctga tccggcccgg gtcaattcgg caatttgttc
    27961 ctccagcgtc atggcgaaag gattaccgga tagatcccgc ggggtcaaga tgcaaaatca
    28021 tgacgcgaca aaatacagga cgccgtggaa aacaagcgcg gctgaaatat ccggcattca
    28081 tgaaaaatat tgataaaaaa aggcaatcca tatataagcc tgaatcaatg tttaaaggag
    28141 ccgtcccatg ttcggtattg gagtacagga actgattatc atcgcgatta tcgcgctatt
    28201 gatcgtcggg cccaaaaagc tgcccgatct ggccaagacg ctgggcaagg gcttccggga
    28261 cttcaaaaat gccacggaag gcgttacgga ggacctgaaa gacgccttga aagaagacga
    28321 taaacccaaa cccgatgacg gtctgaagga ttccctgctg tccaaaaaac cggaggcgga
    28381 agaggaaaaa aacaaagccc ccggaaatga aacctgaact tcaacatcat ttctgcgatt
    28441 tcaagcccgg cacaacaggc gcgtgatgga aaccaacgaa gatcttaaaa tgtctttgac
    28501 cgaacatttg atcgagctgc gcaaacggct gaccaactcg ctgatcgcgc tgggcatcgg
    28561 ttttctggcc tgttatgctt tcaaagatta cctgtttgcc gtcatcacga agccgctgac
    28621 ggacgctctt cccaaaagca gttacctgat tttcaccggc ctgacgcagg ctttcttcac
    28681 ctacatgaag atcgctattt tttcttccct ggtgctgacc agccccttca ttttttacca
    28741 ggtttggaaa ttcatcgcgc ccgcgctgct tcccgaggag aaaaagtatg tggcgccttt
    28801 tgtcctgtcc tccacgctgc tttttgcagg aggcgtgctg ttcggctatt tcgttgtgct
    28861 gccgccggct tttgaatttt ttgtcagctt caacaacgaa tacctgcggg ccatgatctc
    28921 cttcaacgac tacctctcgc tgttcattaa attcctgctg ggttttggtt tgtcgttcca
    28981 gctgcccgtg ctggtcttct ttctggccaa actgggcatc gtcaacgaca agctgctctc
    29041 ccgataccgg aaatacgcca tcctgctcat gtttattgcc gccgcgattc ttacgccttc
    29101 accggacgcg ctcagccaga ttctgatggc gatccccttg ctgtttctgt atgaagtcag
    29161 tatatttgtg gcaaagtttg ccgccaggaa gaaagaagcc cccccgcaac ccgaagcgcc
    29221 gggcgcagaa gaggaagttt gaagatgtct ccagacaaag atagaaacgg tcgacgcaaa
    29281 acgggaagca aacggtcccc cgcaatcaaa atcaccctgg cggtgttttt ggttctcttt
    29341 ttggctgcgg caggcggggc cgtgacctac cgggtcatga cgaaggactt gcccggcatc
    29401 gacgcactca aggattaccg ccccagcatc gcttcccgcg tgctggacga aaacgacatg
    29461 ctgatcgacg agtttttcct ggaagaccgc aagatgatca atatcgccga cgtcccgaaa
    29521 atcgttcagt acgcgtttgt cgcggcggag gattcccgct tctacaagca ccggggggtc
    29581 gatttgctga gcattttccg cgcgctgttc aaaaatgtgg aagcgggcaa aatcgtgcag
    29641 ggcggcagca ccatcaccca gcaggttgcc aagctgatgt acctgacgcc ggagcgaaaa
    29701 tatgtccgaa agctgaagga agcgatcctg tcctaccgca tcgacaaata cctgaccaag
    29761 gatgagattc tccatctgta cctgaatcaa atttatctgg gacacggcac ctatgggatc
    29821 gaatcggcgt cgatcggcta cttcggcaaa agcgcgcggg acctgacgct gccggaagcc
    29881 gcgcttctgg ccggtttgcc gaaagccccc accacctact ccccgtttct caatttcaac
    29941 cgggcaaaac aacggcagat ctacgtcctg acgcgcatgc aggaagaagg cttcatcagc
    30001 aaagaccaga tgacgcaggc cgcagccgcc cccctgagcc tgaggcccat gaagccgaaa
    30061 gacaaggtcg ccgcgtactt tgtggagcac gtccgccgct acgtgcagga aaaatacggc
    30121 gcggacgtcc tgtacaagga ggggctgacc atttacacga cgctgaatct ttccgcgcaa
    30181 accgcggccc gcgacgcgct gatgaggggc ctttcggaaa tggaagcccg gaacaaatat
    30241 gccaagggcc tggtgcaggg cgcgctgtac tgcatggatg tgaagaccgg cgcgatccgc
    30301 gccctggtcg gaggacggga cttcaccaaa agcgagttca accgggcggt ccagtcgcgt
    30361 cgccagcccg gctccgcctt caagcccctg atctacacgg cggcgttcga caagggcatg
    30421 aatccctcca cgcgattcgt ggattccccg attatcatgg aagacccgtc gcaacccgac
    30481 ggactctgga aaccgaaaaa cttcgatgaa aaattcctgg ggccgattac catgaggacc
    30541 gcgctggtgc agtcccgcaa cgtcgtcacc gttaaaatcc tgcaggaaat cggggtggac
    30601 tacgccatct cctacgcggc caatctgggc attgaatccc ccctgcccgc gaatctctcg
    30661 ctggcgctgg gatcggcggg ggtgaccctg caggaaatgg tccgcgctta cggcgcgctg
    30721 gccaacggcg ggaaaaaagt atcgccctac tttatccgaa agatcgtgga ccgcaccgga
    30781 aatgtttttg aagaaaccaa gcctctgtcg gaacaggtga tcgatccccg catcgccttt
    30841 atgacgacgt atatcatgca ggacgtggtg gaaagcggca cgggccggag agtgaaaagc
    30901 atcggacgtc ccgtcgccgg aaaaacgggg accaccaatg atgtccgcga cgcctggttc
    30961 atcggcttca ccccgtctct catcaccggc gtctgggtcg gcttcgacca ggaaaaatct
    31021 ctggggagcc atgaagtggg cggccgcgca gcggcgccga tctggcttta cttcatggaa
    31081 aaagccctgc aggacaagcc cgcggaagct tttaacgctc ctccgggcat cgtgttcatc
    31141 cgggtggatg ccaaaaccgg caggccggcg ccgggcgccg gaccgggcac cattgcggaa
    31201 tgctttttgg aagataaccc tcccggtgaa aacggaaatg aaatgttcgg ggaaagagaa
    31261 gagctgttcc gctgaaaatt cagccggatc gagaatgccc ttgacaagca aattattttt
    31321 cgctaaagag gaggcgaatt caaaaggacg ttatgatgtt tcttaaagat ttagacagcg
    31381 gcagcatccg gattcaagcc attgtaagac ggctcgtagt cgtcgtacgg atggggtctg
    31441 ccgccgggta tctttaagaa ctgaagatac tagccgcggg ccccgaaagc ccgcggcttt
    31501 gttttataga aacgaagccg ctttttccaa aagaaacggg cggctttttt agttttcggg
    31561 catcgggcct gcaggaaaat aacagggaac gaaggagtca ttctgatgaa aatgaaaggc
    31621 acggaaattc tgctcaaagc gctggaagaa gaaaacgtgg acatcatttt cggctacccg
    31681 ggcggagcgg ttctggacat ttacgatcag ctttacaaaa gcaacctccg gcatattctg
    31741 gtccgtcacg aacagggcgc ggtgcatgcc gcggacggct atgcgcgggc aaccggcaaa
    31801 gtaggcgtct gcctggtcac ctccggcccc ggcgccacca acgcggtcac cggcgtcgcc
    31861 acggccaaca tggactccat ccccctggtg gtgttgaccg gacaggtgcc gacgggactg
    31921 atcggcaacg acgcttttca ggaatgcgac atcaccggca ttacgcgccc ctgcaccaag
    31981 cacaatttcc tggtgcgcaa tattgaagac ctggccgcga cgatcaaaga agcgtttcat
    32041 atcgcccgct ccggccggcc gggtccggtg ctggtggatc tgcccaaaaa tgtcatggcc
    32101 gcgcaaaccg agtatgatcc gggcaatgtc cggatccgca atcacgaagt cctgtacaaa
    32161 cccgctccga aaaaaatggc caacctgctg tccatgctcc aggaagcgaa aaagcccctg
    32221 atcatgacgg gcggcggcgt cattttgagc cgggcggctc aggagttgac ggacctggcc
    32281 agaaaataca ggatccccgt gaccggcacg ctcatgggtc tgggctcctt tccggcgagc
    32341 gatccgctgt ggctgggcat gctgggcatg cacggaacct attacgccaa tatggccgtg
    32401 agccattgcg atctcctgct ggccgtgggc gtgcgcttcg acgaccgcgt caccggttcg
    32461 atcgaaactt ttgctccgga agccaaaatt gttcatatcg acatcgatcc ctcttccatc
    32521 aataaaaatg taaccgtgga cctgccgatt gtcggcgacg cccaatccgc cctgacggat
    32581 ctgctgcgct ttctcgagga gcaccactac gaaccggaca tcgcgtcgag gcaggcgtgg
    32641 ctttccgaaa ttgacgaatg gcggggaaag gtgccgctga cctactgcca gaacggtgaa
    32701 gtcatcaaac cgcagttcgt cattcaaacc ctgcacaggc tcacgggggg cgacgccctc
    32761 attaccacgg aggtcggcca gaaccagatg tggacggcgc agttcttcgg cttcgaccgc
    32821 cccaatacgt tcatttcttc cgggggcctg ggaaccatgg gcttcggact gccggccgcc
    32881 atcggcgtca agtgcgcttt tcccgacaaa caggtcgtgg acatcgcggg cgacggcagc
    32941 atccagatga atattcagga actggccacg gccgcccagt acaacatcgc cgtcaaggtg
    33001 gttctcctca acaacggcta tctgggcatg gtgcgccagt ggcaggaact tttttatgaa
    33061 aaacgctact cccagacgga catgacgtac gcgcccgatt ttgtgaggct tgcggaagcg
    33121 ttcggcgtgg tcggccttcg ggcgacaaaa cccggcgaag tggaagaggt cctgcggcag
    33181 gggcttgcgg tccataagcc cgtgctgatg gatttccgcg tctcgcgcga ggaatgcgtc
    33241 tatcccatgg tgcatccggg cgagtcaatc agccagatgg ccctgggcac ccgggaaatg
    33301 atcaactgat aaaatcgtca tcctaacggc aaagagaggg taaacatgga aaaaaaggac
    33361 catattattt caatcctggt gtacaataag ccggatgtgc tggccaggat agcaggaacg
    33421 ctgggcggca aaggctacaa catcgacagt ctttgcgtga acaccaccac acaaaacgac
    33481 atctccaaaa tcgtcatgac gaccgtcggg tcccaggcga ccatcaaccg gattgtcaac
    33541 gaactgcaga aactggttga cgtgatctcc gccgacgacc tgaccaatgt cgaatccatc
    33601 catcgcgaaa tggtgctggt tcgtctgaac ctgaccccgg ccaatacgga ggcggtcaaa
    33661 aaggcactgg agacccacaa atggaaagtg ctcgtgtccg gcgacacgtt cgtcatgatc
    33721 gaaattaccg gagacaagac gcagattgac tttgcgctgg cgcgactgga gccgctcggc
    33781 gtcaccgata tcacgcgcac ggggctggtg gccttgaaac tggacgatta acaacaacga
    33841 tctataaaga aaacaggggg ttttatggca aaaatcaatt ttggcgggac catcgaagac
    33901 gtggtgacct cggaagagtt caccgtaaaa aaggcgcagg acgtcctcaa gcaggaagtc
    33961 atcgccgtgc tgggctacgg cgtgcagggc cccgcgcagg ccttcaacat gaaagacaac
    34021 gggatcaacg tcatcatcgg ccagtctccg gaagacaaac cttactggga caaggccatc
    34081 gccgacggct ggattcccgg ccggacgctt ttcccgatcg aagaagcggt ggaaaaagcc
    34141 accatcatcc aatacctggt atccgatgcg gcgcagatga ttctctggcc caaagtcaaa
    34201 ccgcacttaa agaaaggcga cgcgctgtat ttctcgcatg gtttttccat tgtgtataaa
    34261 gaacagaccg gtgtgattcc gccggatttt gtcgatgtca tcatggttgc acccaaaggc
    34321 tccggcacca gcgtccgccg taatttcctc gacggcagcg gcatcaactc cagtttcgcc
    34381 gtgcatcagg atgcaaccgg ccgggccatg gagcgaacgc tcgctttggg catcgccatc
    34441 gggtcgggct tcctctttcc gacaacgttc cagatggaag tgtacagcga tctgaccggc
    34501 gaacgcggcg tgctgatggg cgcgctggcc ggcatgatgg aagcccagta tgcggagctc
    34561 aggcgtcacg gccacacgcc cagcgaggcc ttcaatgaaa cggtggaaga gctgacccag
    34621 agcctcattc gcctcgtcgg tgaaaacggc atggactgga tgtacgccaa ctgctcggcg
    34681 acggcgcagc gcggcgcgct cgactggcgt cataaattcc gcaaggcggt ggagcccgtt
    34741 ttcgcggagc tttaccggtc cgtcgccgcc ggcagggaaa cggaaatcgt cctgcgcgtc
    34801 aacagcgctc cg
//
LOCUS       DAOSRM010000008        34653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1378_length_34653_cov_109.191832, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000008 DAOSRM010000000
VERSION     DAOSRM010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 34653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34653
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1378_length_34653_cov_109.191832"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PLA58_01810"
     CDS             complement(<1..539)
                     /locus_tag="PLA58_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOR61561.1"
                     /translation="MSVIKDVIAKVKQTDPDQPEFHQAVEEVMETLEPTVKKHPEFVK
                     ANIYERIVEPERAVIFRVPWMDDKGNVQVNRGFRVQFNNAIGPFKGGIRFHPSVNLGI
                     IKFLGFEQIFKNALTTLPMGGAKGGSDFDPKGKSDAEVMKFCQSFMRELYRHIGADVD
                     VPAGDIGVGAREIGYMYGY"
     gene            complement(580..1581)
                     /locus_tag="PLA58_01815"
     CDS             complement(580..1581)
                     /locus_tag="PLA58_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOR61562.1"
                     /translation="MKELFKPEDIERKVLLILKILRESPDPMGARVIARRMSDYHVQL
                     SERTVRYHLKIMDERGLTKLVGRRDGRVITDQGFAEIGDARVQDKIGLSISRIDLLSF
                     RTTFDVKKKRGLVPVNVSFFPKEHFARALSIMKPVFQQKLAVSSRVAVAEAGKNLGDI
                     AAPEGHVGFATVCSIVINGVLLKHGIPIDSKFGGILQMRGGKILRFVELIHYAGSSLD
                     PSEIFIRGKMTSVRSVAKTGEGKILANFREIPALSRPLVEKVMEDFTQCGIHGVLSIG
                     HTGRPVCQTHVDMNKIGMILIGGLNPVAAVCEEGLPVDNKAMSTVMEYEKMRDIETL"
     gene            complement(1657..5139)
                     /locus_tag="PLA58_01820"
     CDS             complement(1657..5139)
                     /locus_tag="PLA58_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOR61563.1"
                     /translation="MEKIFNGQTDPADYLDGRETIRALTNCVKELKRENVQEYLFFHQ
                     ERAVNLTRMTAGMKAFLADEEKILEERAAGISSADLETIHRRLLLFKDICWMLDCDIL
                     TNLQKVLSLSGAEKCSSLQPAAFRKYRKLNLLLNALDRLEVRGRDSAGIQLAFVLKNE
                     SAIEELVRRIEETGWGEDYRRRTQKGDLLNRSIFISTGPGGHGGGIHVAFTYKTFSVV
                     GELGRNVADLRQVIRRDGILRLFAEQASMDESAIAHTRWASVGSITEENCHPVNNAAV
                     RPEPPVFPAYPGLEAHISVVLNGDIDNYPALRRDLEKDGEEIAPDVSTDTKIIPLQIE
                     KYLKAGRNLTDAFRLAVKDFEGSHAIAMQSNLEPGKMFLALQGSGQSIYVGVSEDAYM
                     VSSELYGLVEVAPRFIRMNGDAGSGTAGGQLFILDQDRGGGSKGIAACFYDGTPIRPE
                     DQNLQTAEITTRDIDRGHHPHFFLKEISESGLSVRRTLRGKYRIIDAGSAPPQVLFNL
                     GEDIVPASVRNGLQSGKTQNICVIGHGTAAVAGQAVADALAHCLKGSRLNIQSRVASE
                     LSGFGLREDLSDTLVIPITQSGTTTDTNRAVAMARERGAQILSIVNRRQSDITASSHG
                     VFYTSDGRDIEMSVASTKAFYAQMVAGQVLALYFAGLLGTLSDEQIVRRLRHLESVPQ
                     CMQRLFDRRDTVAASVKQSVGKRYWAVVGSGPNKAAADEIRIKLSELCYKTISSDIVE
                     NKKHIDLSAEPLILVCAAGNPDVVLEDIAKDVAIFRAHKAATVVFADEGDLRFDSVAD
                     AVIPIPPAPAPLAVILNAMAGHLWGYYAACAIDEEAQLFRVFRSRLATERTRKGKTKL
                     SIFDVFDLMADVSFCRIINDFYLLFQSRNREGALGALGGKAIADLTLLLKYASGKLPM
                     QDIRQEFHGQEDLVHPFDLLDEALGTAIDELTRPIDAIRHQAKTVTVGTSRREKELGG
                     VIFDLLETLNFSAKNLSYRNLRTIHRLQPAAAAVRGYTVYSVGGLDEQGHPGPDATIA
                     VCAKGGIALRMNSRAEQPTTLMGVKKTIVSTGHVYLGRGKTDSSSIMVIPLQGADGRT
                     GNLLLIHITFNENLSGPEKIEALGHRYDDIRNLINEYNLIWNDRYLEGLSLEFLFSEP
                     IEEIARKIQARRSP"
     gene            complement(5897..6193)
                     /locus_tag="PLA58_01825"
     CDS             complement(5897..6193)
                     /locus_tag="PLA58_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR61564.1"
                     /translation="MKAKKQPKPATGMNVQETLNKAVEEILEKKLDDLATILSGSDNG
                     KSKLHAEVMAMVETGLFKIALRRSGNIKSTAAAFLGIHRNTFTDKMTKLGLDGE"
     gene            complement(6283..6792)
                     /locus_tag="PLA58_01830"
     CDS             complement(6283..6792)
                     /locus_tag="PLA58_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61565.1"
                     /translation="MSVYKIKKTFRFPFVAVTVLLFALFATGMAMGELWERILTGVLF
                     IIALVVAVEVSEREFRTSENGLQIRKFFRTKNFAWAEITHLGTVLLRNKAYFLLTTTK
                     GFYIFSNLLQDHTLLLKFLAEKLGGEKVEAEVKSYIEAPRERTSLIVLTWVSLVILVA
                     LLLTRWLAA"
     gene            complement(6807..7292)
                     /gene="moaC"
                     /locus_tag="PLA58_01835"
     CDS             complement(6807..7292)
                     /gene="moaC"
                     /locus_tag="PLA58_01835"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOR61566.1"
                     /translation="MKKLSHIDDAGQVQMVDVTDKAQTARKAKARGQVTMKSATLKLL
                     AEGRMAKGNVLTAAKLAGILAAKKTGDLIPLCHPLGLTGVDIAFVIDRKKSRVIIESQ
                     AQTVGQTGVEMEALTAVSVAALTIYDMCKAVDREMVIGDILLLEKSGGKSGAFVRKTA
                     N"
     gene            7472..9001
                     /locus_tag="PLA58_01840"
     CDS             7472..9001
                     /locus_tag="PLA58_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOR61567.1"
                     /translation="MFVKVQSMSVIGMESYPVTVEVDVSQGLPQFATVGLPDASVKES
                     RDRIKAAIKNSGYAFPRDHVTVNLAPADIRKEGTGFDLPIAVGILAAEALIGEAALDG
                     CLFIGELSLDGSIKGVRGVLPAAFKARESGIRSIFVPEENAAEAAMVEGVCVYGVRSL
                     PDVVEFLGDRKTIEPLCVNADDLFQINRRYDIDFSEIKGQKLALRALEIAAAGSHNLL
                     MIGPPGSGKSMLARRLPTILPDLSFEEAIEVTKVFSVAGLLSAGETLIAARPFRAPHH
                     TISDVGLVGGGQMPHPGEISLAHLGVLFLDELPEFKRNALEALRQPLEDGTITITRSA
                     VTTAFPAKFMLVAAMNPCPCGYFGDRVRRCRCSPQQIRQYQAKISGPLLDRIDLHVEV
                     PSVRYRALTGKENNESSAEVRKRVVGARRRQQERFGGAAIPANARMTEKQIRSFCSID
                     EESHQLMEMAVEKLGLSARAMNRILKVSRTIADLDGAEKIGAAHVAEAIQYRSLDRNI
                     V"
     gene            9062..10132
                     /locus_tag="PLA58_01845"
     CDS             9062..10132
                     /locus_tag="PLA58_01845"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOR61568.1"
                     /translation="MKIKIIKALPKYLKPKPDEKKLGFGQHFTDHMFTMKYREGDGWY
                     DPVIEPYHYLQLDPTAMCLHYGQEIFEGLKAYRGTNDEIYLFRPLENIRRMNVSAERL
                     CMPPIDETVFLDALKQFVVLEKEWIPREKGASLYIRPTMIATEPALGVHPASEYLFFI
                     ICGPVGAYYAHGFGPTKIYVSDDCVRAAPGGTGHVKAAGNYAASLYASRIASAMGYTQ
                     VLWLDARENRYVEEVGTSNIFFMIGDRLITPPLGGTILPGVTRDSVLKLAAHLGVKAE
                     ESRIAITEVISAIEEGTLRDAFATGTAAVISPVGQIFYHNREYLINDGKTGELAEKLY
                     HEILQIQYGLKEDPFGWRMRIA"
     gene            10447..11148
                     /locus_tag="PLA58_01850"
     CDS             10447..11148
                     /locus_tag="PLA58_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOR61569.1"
                     /translation="MKVNLNKIELFMPAALLAVCAALFLGVACPAASPAGPAPALEGV
                     IDRLQQIYEKTQDFQAGFIQETTVKSIRKTDTEAGTVYFKNPRQMLWDYKKPKTKKLI
                     INAQKAWLYLPGEKTVYTQESNKIFKSEALIKFLSGLGKLNHDFTIQYASPAVDLDGN
                     YLLLLYPREKGASYSCLKMTIAKNNFYILQVGFDDVMGNSTLLKFSDMKMNSGLPSKL
                     FQFHPPPGVSIFKMP"
     gene            complement(11162..11578)
                     /locus_tag="PLA58_01855"
     CDS             complement(11162..11578)
                     /locus_tag="PLA58_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOR61570.1"
                     /translation="MKKYIIDTNALISFVTDRNPDQQEIIADLFEQAAQIKVFLFVHQ
                     HTLTEFIFVMEKVYSIPKKEIARMISDLIAMPGIEVVNTVDYSLVLSWWPESVPDFGD
                     AVIASICKNIKGSIVVTFDRKFMERLKALKLNFQTF"
     gene            complement(11575..11808)
                     /locus_tag="PLA58_01860"
     CDS             complement(11575..11808)
                     /locus_tag="PLA58_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOR61571.1"
                     /translation="MQSVITSKYQTTIPKAIRDNLGLSVKDALEWKLENGKVVVYPSR
                     LQFLKHRNALKVGKGDIAADIELAREKRLEKYR"
     gene            complement(11884..12501)
                     /locus_tag="PLA58_01865"
     CDS             complement(11884..12501)
                     /locus_tag="PLA58_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOR61572.1"
                     /translation="MSGDLRQIYDRFADTYEQNRGLFDMSEVIDDFSRRLPQRPGRLL
                     DLGCGAGEPFPAFFITRGWRVTGVDFSSRMLELARHYQPNMETVEADIRNVEFPDRQF
                     DAVTAIYCLFHIEHEKHGGIFQKIFRWMKPAGKLLFTYATKEYTGAEIFNGYKEFMGE
                     KLFYSHTTPGNLAAILKSIGFVIESSRYRQIGGETFLWITAAKPG"
     gene            complement(12506..13435)
                     /locus_tag="PLA58_01870"
     CDS             complement(12506..13435)
                     /locus_tag="PLA58_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61573.1"
                     /translation="MFMIIPELLDMYRSCALCPRACHVDRTKGEPGYCRLPSDIVMDC
                     ALPHHGEEPPLSGTGGAGTIFLSSCNLGCVYCQNYQISHRAQGRRLTVIELARIMIDL
                     QKSGCHNVEPVTPTPQTPLVMEALCLARAQGLTVPLVYNCGGYENPDVIRMLDGMVDI
                     YLPDFKYGLEEDSLAFSNAPQYPRFALASLREMVRQVGDDLDIENGVAVRGIIIRHLL
                     LPGRLENSKEALRLLRGEISARIPISLMSQYTPIPKVRSHPLLSRRITAAEYHEIVEF
                     ALSLGFENLFLQEVDERALTPDFNRENPFDKTG"
     gene            complement(13577..14344)
                     /locus_tag="PLA58_01875"
     CDS             complement(13577..14344)
                     /locus_tag="PLA58_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOR61574.1"
                     /translation="MEYKNIIVTTENFVTTIVLNRPPMNSVNLGIREEFFDAVTNIEK
                     SKETRAVIITGAGEKGFSAGMDVSDVANITRGPNGNDIMNAISRSTKPYIAAINGHAL
                     GGGCETAIACHFRIMTDNPKALIGLPEVNLGITPGWGGIQRLPRLLGKSKALELILFS
                     KRLPPTEALAVGLVDKVVPAADLMKEATAFAQALTKRPPLAVAAVLEGISVGMEKGFN
                     EGLKLDIEWSNKLAASKDAVEGITAFLQKREPNFTGE"
     gene            complement(14441..15316)
                     /locus_tag="PLA58_01880"
     CDS             complement(14441..15316)
                     /locus_tag="PLA58_01880"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOR61575.1"
                     /translation="MKIELQQMRTIGCGLTRPEGVMALDDGSVRTADGHGRCSRIDPD
                     GRTAFYGRLGGLPNGICMDTEGNCIVANIGNGEVQSLSPDGRHTVLMTEAQGKRMFTP
                     NFPFLDFAGRLWVSNSTDNPDIDASLRAPIPDGCLVVIAKGRAPQIVASGICFANGVA
                     VDAEEQYVYVAETMKRRVLRYRIHADDTVGPAEVYGPEFLGKFGFPDGIAFDEAGNLW
                     VAFPVANAVGYIDPQGALNLYAEDQQGIVLSRPANICFGGKNRRTAFIGSLGGTNVPC
                     FEVPYPGMRLVHQEN"
     gene            complement(15306..16481)
                     /locus_tag="PLA58_01885"
     CDS             complement(15306..16481)
                     /locus_tag="PLA58_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOR61576.1"
                     /translation="MSILFESARLKNMTLRNRFVRSATYDGMSDPTGQVTDRQINLIA
                     GLAEGGVGLIVTAITYVHPSGQVSSFMNSIAEDSCIPGLQKLARAAHQRGAKIAVQLY
                     HGGREARFVKTRGQLPIGPSVIADDPFYRWNYREMTTREIEDVVQAFGRAARRAREAG
                     FDAVQIHGAHGYLFSQFLSPFTNRREDAWGGSLENRLRLHREVYASVRDHAGPDYPVG
                     IKLGVEDGFKGGLTFSEGMKAALMLAETGYDALEISGGVRGEKYEGTEYRTKINIPSR
                     EGYFRAWTGEIRKKTDTPVIAVGGLKSIAMMEEMIRGGEADFISLCRPLISEPGLIRS
                     WEADPGKKPRCVYCNQCLEAVHRGIPLSCVAFSKGEQTVLKAPQRNVGANPEGTHEN"
     gene            complement(16539..16808)
                     /locus_tag="PLA58_01890"
     CDS             complement(16539..16808)
                     /locus_tag="PLA58_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61577.1"
                     /translation="MSKAVKNSGIDVKEIKIRKPMLTMRLNIENPFAFGYFPVRMARS
                     IDRRSKPVNHIPSQGQKPDLRAQILVPAEKNDPAFHKPLPETAKS"
     gene            16855..17478
                     /locus_tag="PLA58_01895"
     CDS             16855..17478
                     /locus_tag="PLA58_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR61578.1"
                     /translation="MFTCFWASIYTWTMPAWRIRIEGRDNVRKDAAYMVVSNHQSQLD
                     ILIAFRLFFHYKWVSKIEIFRVPLIGWNMMLNRYIQLKRGDKQSIEQMLSQCAMHLDE
                     GSSIFMFPEGTRSHDGEVKAFKPGAFQLAHQKKVPILPIVISGTNKALPKYSMNFTGV
                     QKIYIKIFEEIPYDAFAHLSVGETAEMVRGFISRQLRLMNEYTLGNV"
     gene            complement(17501..18616)
                     /locus_tag="PLA58_01900"
     CDS             complement(17501..18616)
                     /locus_tag="PLA58_01900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR61579.1"
                     /translation="MKQPDKPFDMPALAADFAARHVACRADLQTEQEFPSDLWRKMGK
                     AGLFRGGLPEEYGGDGGGYLDLLRSGEAFVRSGLNLGLGMSWIFQQIIARSVVGTFGT
                     TEQCRQYLRAAALGNCAISFAVSEPGRGASPKALATKAQQQGESYLLTGEKTHLTNGP
                     IADLFVVVAVTDDTSARKGFTAFMVPRGAQGLFVEPPMPLNFLKPSPHGGIQLNGCLI
                     PKASVLGREGNAWEDIVVPLGETEDVVMMGPVLGGMAAQLDLLNSALRGHSTDRTLQG
                     EWGALQALWQALRSIAYEAAGKLDRGDGSTASAGIAFARFAAEFHADIARWPEQLHLQ
                     PPERFGLLQKDMAFLEMLQKRRLQIRREKIGAALLKM"
     gene            complement(18613..19998)
                     /locus_tag="PLA58_01905"
     CDS             complement(18613..19998)
                     /locus_tag="PLA58_01905"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOR61580.1"
                     /translation="MVKLIGQFVSCCRAAGLRVSTSEVLDTLKQLELIDPTDELQFKA
                     LLRANFAKSLRELKHFERLYHLFFHEMRVNAEDMQQAASQIDRIREATDLLRKKPHDN
                     PMYDAVVDFLAGNPMALLAEMRRIHLESDDAAAPARFNLGPLASRFNVLLQINAAANA
                     AAALLENNELNMTPADRHNLAAHFEERVEAARSILVYEPQAGDGGAKKVKTHEQRVRD
                     LGEKAFSSFTREEVEEMREAIDRLVRKLKNIVSRRFAVRDRGNLDVKKTLRASAKYDG
                     VPLDIQYRRKSLRKSRIVTLCDVSGSVWAAARFMLNLVYSLQDCFDKVNSFVFIDQLT
                     NVTGIFEEQDISEAIRIVMEEADVHYSAPTDYGETLRNFKRDYMDLLTKKTTLIIVGD
                     GRSNYMNPEDAILGAMRERCRRVIWLNPEPEKLWGTGDSEIRTYLRHCHEVRACRNVN
                     QLFTFIEELVI"
     gene            complement(20123..21040)
                     /locus_tag="PLA58_01910"
     CDS             complement(20123..21040)
                     /locus_tag="PLA58_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOR61581.1"
                     /translation="MSYKTVSDVEQRLIEAGYIPSREIATVIFLAQATQKPVLIEGPA
                     GVGKTELAKAVARSLGRELIRLQCYEGLDEAKALYEWEYAKQLLYTQMVKDRINDIIG
                     ATATLAEAVDAIAAQEDAFFSDRFIQPRPLLQAISSAKPVVLLLDEVDKSDPEFEAFL
                     LELLSDFQVSIPELGTRTAVRIPFVLLTSNNYRDMSDALKRRCVHLYIDYPARDTEIS
                     IVRLKIPDIEERLCAVLVDSIRKIRTLDLKKSPSISETLDWAQSLLALQITELTPEVI
                     SETLNVICKYQADLEKVRKNIDRVVAGAG"
     gene            21339..22592
                     /locus_tag="PLA58_01915"
     CDS             21339..22592
                     /locus_tag="PLA58_01915"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOR61582.1"
                     /translation="MTWEAMANIFQGVTTLAESFSHNPIMAAARIGLILLGLLMIYLG
                     RKGILEALLMIPMGLGMATVNAAIMFFDPLNLHGGTGTLFVEAQAGATADAVKNAAEI
                     IAILQIDWLQPIYTFTFSNGLIACLVFMGIGALLDVGYVMARPYQSMFIAVCAELGTI
                     VTFPVAQLFGYTPQQAAAISIVGGADGPMVLFTSLRLAPELFVPIAIIAYLYLGFAYG
                     GYPYLIKMLVPKKLLGIKPAPPKKKRNITSGEKMLFAVVACVLLSFLLPVAAPLIFSL
                     FFGVVIRESGIKEFIDLLQGPILYGSTMFLGLMLGVLCDANTIMDPKVLPLLLLGVLS
                     LLISGIGGILGGYILYFVTRGKFNPAIGIAGVSCTPTTSKVAQKVVSKVNRGAIILPD
                     ALGANVSGVITTAIIAGIFCSLLAK"
     gene            22606..22731
                     /locus_tag="PLA58_01920"
     CDS             22606..22731
                     /locus_tag="PLA58_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61583.1"
                     /translation="MSGWPEALLTYGLTIVIAFGVAGMIHLMVVLLEKFSPKKDN"
     gene            22825..27063
                     /locus_tag="PLA58_01925"
     CDS             22825..27063
                     /locus_tag="PLA58_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOR61584.1"
                     /translation="MTTKSPSTEKSDSWVSSALKVNLERTAAEVRIPPQYAPLLQMVE
                     DHYGLQKKTRELLTELNHPYVNWDFVLRELKSISIGNFHLYSRRADGLAGLWILLTIY
                     FDILKSTASEDVKDSALHYLFDYADTVILHSEEFLERNLSLFPGMIESLTALADGESA
                     LFKKCSSYLKRILRTAADRNVDLATPAFDALLYKMFRATYAFWLNQPDPALWLVAERK
                     AEAPPNETPYLELIRPLSHHRFRKLMLELEALRPRDGEKAGAHLRDFLALPDYFQILD
                     TYLRVASALEKSEAYASRHLVKLDFLFGIMTAPGLQDLHVSAMREINASLKLVFQEEK
                     KENLDIFVRKIFGFLKKNTSRSEFRSASIDCILTAAREVFAQNDHPLVETFMDELIAY
                     GFEYPEIQGSTAEWQVRVNPEHIRTIRAWLEIIALKPRWTKRLVSALIIHLKIGGIFI
                     RDTDLIQRDISRLLNADIAPAYSLIKQLLRLFPVYFSEIGAEGELRDLTTRVDELSCR
                     NDRLIDFFRKQSHVESNSLLVEFIEDIFRFWVSGEKRTIRKHVPEEIYDQVANEGLYF
                     DGMHQALTRLFEGIGNHPGKFLEWDPEKIAGEISRIPNISETDRERASLMIRIYQLIY
                     KKYHPRYYDLLGDLESANVFTAQDLLSLKRSLADKNHYRSLTVILKCLGSLKAQILSE
                     KKTRSFENIYYKRHIAAGIPSMYGTYHEEKFDALGLSLRLESLAGMLFEELIQSLNLQ
                     FITKRTIIRIHNYLWTYLNALDLEGISTEGLVAKVRYITSALPIKQFSMEQYLDIFRF
                     ISRGIQDVIRDYYIDAHSVNLPVIIRQLQAQTDPSDGKQDETRMEEVIYQQSENFLRG
                     VIASAFGLQALDNFVHTVIKTLNAELEKFKDNKRIVNLLMDYNPELAVSPLYSKNPKT
                     DNQILLGNKGYFLKKLVSFGFQVPPGFIITTEVFRGFDAVYGYKYIFRDLARRVNREI
                     AALEKKTGRRFGDPGNPLLLSVRSGATVSLPGMMRSFLNVGINASIAETLSARKDFQW
                     AAWDSYRRFLQTWGMFQGLSRDFFDAIMDGFKQKHGVQRKIQFPPGLMKQIALVYKQG
                     ILDSGIPLSDHPLRQLRHAVLKVFDSWYAEQARIYRHQMHLSDAWGTAVIVQAMVFGN
                     LHERSGSGVIFTRDPKSASSDVALYGDFIFGVQGDDIVSGLVETYPISERQRLAEQRN
                     TGISLEADFPAIYAELVKTAETLIYEKGLNHQEIEFTFEGPQKEHLFLLQTRDMDQTQ
                     AKSLRRFKETDLLHASMLGTGIGVAGGALCGRAVYSEKDIQQFRQAEPRTPLILVRPD
                     TVPDDVGVLLQVDGLLTAKGGATSHAAVTIPQLGKVGVVGLSKLKVYEMEGYATLEGR
                     AIQSGDFIGIDGWSGALYLGKHDLESASYASLVP"
     gene            complement(27348..28619)
                     /locus_tag="PLA58_01930"
     CDS             complement(27348..28619)
                     /locus_tag="PLA58_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOR61585.1"
                     /translation="MSNSSAKESRLPVLGINSLGRIGKLTLWHHVNRKYFKEIIVNQG
                     RDIGMGMETIARLIEADATYGLLHRFLYGIKAQPCIQITDEKNGKMLIDGIPVTVLRE
                     QRNPMNIPWRQYGADIVVDCSGSFKDPTVPVDDKKGSIRGHLNGGAKAVIHSAPFKIK
                     NKALSTPEDTTTLIYGINHTAFNPKKHLLISAASCTTTGLAHMVKPLLDNKETSTILT
                     ASMSTIHAVTNTQSVLDKLPKAGEKDIRKTRSILNNIILTSTGAAKALAEVIPEVKNI
                     GFMGDSIRVPTNTLSLIVLNATFQARNNDKAAAAGLDTKKLNDIYAKAAQDNPLVRFT
                     MQQNVSTDLIGEDAAVIIEGQFNHTRTAFIPVDLSHIPNLPADLVSSLGEKMLQVPVV
                     HAKIFGWYDNEYGSYTNRMGDLTVYIHKNIQ"
     gene            complement(28944..29270)
                     /gene="hgcB"
                     /locus_tag="PLA58_01935"
     CDS             complement(28944..29270)
                     /gene="hgcB"
                     /locus_tag="PLA58_01935"
                     /inference="COORDINATES: protein motif:HMM:NF040864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HOR61586.1"
                     /translation="MGSMIYLKDVATLALDSQKCTGCGMCLDVCPHGVFKMNGQGLQI
                     ADRNACMECGACSRNCPAGAISVQSGVGCAAAVINSMLGRNGGECCCGPQTGQNQTAK
                     SGGCCC"
     gene            complement(29276..30451)
                     /gene="hgcA"
                     /locus_tag="PLA58_01940"
     CDS             complement(29276..30451)
                     /gene="hgcA"
                     /locus_tag="PLA58_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_264175424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HOR61587.1"
                     /translation="MEENTKGLDAGGKFEFPIIQEQAGCGCGGDNAADADDSGSSLDQ
                     PFVSGSLSTPAGLLPKVSSRLTRQDRWGTIKARFGVGRMNYAVHPGLYALGEPDENAP
                     VLVTANYKMSFDCLRQALPGRNAWILVLDTKGINVWCAAGKGTFGTNELVYRIEASGL
                     KKVVRHRKMVLPQLGAPGVAAHQVKKLSGFKAHYGPVRARDIPAYLDNGLRASREMRI
                     VTFPLADRAVLIPIELLGAAKPFALLSLALLVLAGILGRSGFQTNLLHDGVFAVVALF
                     AALFGGAVLTPLLLPWLPGRAFSVKGLSIGLVTALALMMARGVDVGTWAGRVEILAWL
                     LIIPAIAAYLAMNFTGASTYTSLSGVKKEMRFALPLEITAGIVGLMVWITSLILRWE"
     gene            complement(30467..30895)
                     /locus_tag="PLA58_01945"
     CDS             complement(30467..30895)
                     /locus_tag="PLA58_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOR61588.1"
                     /translation="MNVFVGLAQATRSFRQDAAFCEGVTFHQYIILDAVAKNEELNIS
                     DLHRILSVEKSTTTRLVNPLIQKGLLTRQKSSRDSRAFVLRLTKEGRNTHQNVQLCLE
                     GFFNKIADHLPGDRSDDILQSVQIFLNAVRNAAGICDCCQ"
     gene            31174..31623
                     /locus_tag="PLA58_01950"
     CDS             31174..31623
                     /locus_tag="PLA58_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOR61589.1"
                     /translation="MKNHAAIAEEKFTGGYNCAQSVLFSFCEELGLDADTALKIACGF
                     GAGMGRKGEVCGAVSGGVMVLGLKYGRGEKEERAQTDALYAKVRELIDRFSAKHGSCI
                     CRELLSGCNLATPEGQTYYRENQCFHGICRPCVRDAAEIVADLPAIR"
     gene            31772..32554
                     /locus_tag="PLA58_01955"
     CDS             31772..32554
                     /locus_tag="PLA58_01955"
                     /inference="COORDINATES: protein motif:HMM:NF005126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOR61590.1"
                     /translation="MTINVKTEEHIQHIEVDRPKKFNAMTQGMYHELAQAYFRLDADP
                     EARVGLVYAVGPHFTSGLELTDWAGTFDAGKGFPFVAENEIDPFYMMSDKRCRKPIVL
                     AVHGYCYTWGFEMMLNTDIRVAATDTRFGMLEVKRGFFPGAGATLRLAKEIGWANAMR
                     YMLTGDEMSGPEAYRLGLVQELAEPGQQFDRALALARHVAKAAPLGVAHALRLSRLAV
                     LEGEETARGLIYPAMEEVMKSEDMMEGLQSFIERRAAVFKGK"
     gene            32834..>34653
                     /locus_tag="PLA58_01960"
     CDS             32834..>34653
                     /locus_tag="PLA58_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOR61591.1"
                     /translation="MAHSNWLYQTRDIMFQIKEWLGVEKLLALDAYKDYYGMDDINSI
                     LDVNYKVCRDVMCPANKDADEPGAKFVGGNEHAVTTPDSFKNVYKTIMDAELGPQFGY
                     RGEGKIPLCWYAPILEMQSAASPSIVMFWCLTQGAITVLHEFATQKQQDLFIPKLRTG
                     EWGGTMGLTEPGAGSEVGAVQSKAFPTDTPGKYKLTGTKCFISSGDHDLAENIIHLML
                     AKTPGAKEGTAGISLFIVPKFWVNEDGSLGAWNDVTTMGIEHKLGIHGSTTATLSMGE
                     NDNCYGWMVGEAEVKDGRGIGMKQMFAYMNEERLNTGLFSLGCIGSAYYAALDYAKLR
                     VQSKHSTNPKGPSVRIIEHEDVRRMLLSQKAVLEACRALIYKSYLYRDLAVDAATPEE
                     REYYDDMFAINNPMCKAYTSDMARLTTGDAIQCHGGYGFTEEYAPAELYRDVVIYGIW
                     EGTNFIQAQDYTGRKFTMKDGAPFKKWVAEIDDFVAKKKTDAFAAEFAMMAEAMTAFK
                     EIVHMNDAWTKGDRQMKQLFATRTLHAGARVYCGMLLLDQALLASARMKEKGEIDADF
                     YKGKIATARFYIMNHVPELFGYLKSMKFGDRSAIDIAEES"
BASE COUNT         7401 a         9477 c         9792 g         7983 t
ORIGIN      
        1 tagtagccgt acatgtagcc gatttcccgc gcgcccacgc cgatgtcgcc ggccggaacg
       61 tccacatccg cgccgatatg gcggtacagc tcgcgcatga aggactggca gaatttcatg
      121 acttccgcgt cggatttgcc cttcgggtcg aagtccgagc cgcccttggc gccgcccatg
      181 ggcagcgtgg tgagcgcatt tttgaagatc tgctcaaatc ccaggaattt gataatgccc
      241 agatttacgg acggatggaa gcggatgccg cccttgaacg ggccgatggc gttgttgaac
      301 tggacgcgga agccgcggtt cacctgcaca tttcccttgt cgtccatcca gggcacgcgg
      361 aagatcacgg ctctttccgg ttcgacaatc cgttcgtaaa tgttggcctt tacgaattcc
      421 gggtgctttt tgacggtagg ctccagcgtt tccatgactt cttcgaccgc ctgatgaaat
      481 tccggctggt ccggatcggt ctgtttgact ttggcgataa catctttaat gactgacata
      541 gtttgattcc ccctgtagag tagtatggtt ttttaatggt taaagcgttt caatatcccg
      601 cattttttca tattccatga cggtggacat ggctttgttg tccaccggca ggccttcttc
      661 gcagaccgcc gccaccgggt tcaggccgcc gatcagaatc attccgattt tattcatatc
      721 cacatgagtc tgacagaccg gccggccggt atggccgatg gaaaggacgc cgtgaatgcc
      781 gcattgggtg aaatcctcca tgaccttttc aaccagcgga cggctgagtg ccgggatttc
      841 cctgaaattg gccagaatct ttccctctcc ggtcttggcg acgctccgga cggacgtcat
      901 tttcccgcga atgaagattt ccgacggatc cagcgaagag ccggcgtagt gaatcagttc
      961 cacaaaacga aggatttttc cgccgcgcat ctggagaatg ccgccgaatt tggagtcgat
     1021 cgggatgccg tgctttaaca ggacgccgtt gatgacgatg ctgcagaccg tggcgaagcc
     1081 gacatggcct tcgggcgccg caatgtcccc caggtttttt ccggcctcgg ccaccgctac
     1141 gcgcgagctg acggccagtt tttgctgaaa cacgggtttc atgatggaaa gcgcccgggc
     1201 gaagtgctct ttcggaaaga aggaaacatt gaccggcacg aggccgcgct tctttttgac
     1261 gtcaaaggtg gtccgaaacg agaggaggtc gatgcgtgaa atggaaaggc cgatcttgtc
     1321 ctgcacgcgg gcgtcgccaa tttcggcaaa cccctgatcg gtgatcacgc ggccgtcgcg
     1381 gcgtcccacg agtttggtga gaccccgctc gtccatgatt ttcaggtggt agcggaccgt
     1441 tctctcgctg agctgcacgt gataatccga catccggcgg gcaatgacgc gcgcccccat
     1501 cggatcgggg ctttcacgga ggatcttcag gatcaggaga actttccgtt caatgtcttc
     1561 gggcttaaaa agctctttca ttcgctttcc gtaaggctat cggcaatcgt ttgccgtatc
     1621 cctactttca aaccgttttt ttgtcaaata aaaattttac gggctgcggc gagcctgaat
     1681 ctttcgtgca atttcttcga tcggttcact gaacaggaat tccagcgaca ggccttccag
     1741 atatcggtcg ttccatatca ggttgtattc attgatcaaa ttgcggatgt cgtcataccg
     1801 gtggcccagc gcctcgattt tttccggccc gctgaggttc tcattgaagg tgatatggat
     1861 cagaagcagg ttgcccgtcc ggccgtccgc tccctgcagg gggatgacca tgatggaaga
     1921 ggaatcggtt ttcccgcggc ccaggtaaac atggcctgtg ctgacgatgg ttttcttgac
     1981 ccccatcagc gttgtgggct gctccgcgcg ggagttcatg cgcagggcga tgccgccttt
     2041 ggcgcatacc gcaatggttg catccggccc gggatgcccc tgctcgtcga ggccgccgac
     2101 gctgtaaacc gtgtatcccc ggaccgccgc agccgcaggc tggaggcggt gaatcgttct
     2161 gaggttacgg tagctcagat tcttcgcgga gaaattcagg gtctcgagca ggtcaaaaat
     2221 gacgcctccg agttcttttt ccctgcggct ggtccccacg gtcacggttt tggcctgatg
     2281 gcggatggcg tcgatcggac gggtcaattc gtcaatggcg gttcccagag cttcgtccag
     2341 cagatcgaaa ggatggacca ggtcttcctg tccgtgaaat tcctgccgga tatcctgcat
     2401 gggcagtttt ccggatgcgt acttcaacag aagggtgaga tcggcgatgg ctttgccgcc
     2461 cagcgcgccc agcgcgccct cccggttccg ggattgaaac aggaggtaaa aatcgttgat
     2521 gatccggcag aaggaaacgt cggccatcag gtcgaaaaca tcaaaaatcg ataatttcgt
     2581 ttttcccttg cgcgtccgtt ccgtcgccag gcggctgcga aacacccgga agagctgcgc
     2641 ttcctcgtcg atggcgcagg ccgcgtaata gccccacagg tggccggcca tggcgttcag
     2701 aatcacggca agaggagcgg gcgccggggg aatgggaatg acggcgtcgg caaccgaatc
     2761 gaagcgcaaa tccccttcat cggcgaaaac gacggttgcc gctttatgcg cccggaagat
     2821 ggcgacgtcc ttggcgatgt cctccaaaac gacatcgggg ttgccggcgg cgcacacgag
     2881 aatcagcggt tcggcggaca ggtcgatgtg ttttttgttt tccacgatgt ccgaagaaat
     2941 ggttttgtaa cacagctcgg acagtttgat gcggatttcg tcggccgccg ccttgttggg
     3001 gccgctgccc acgacggccc agtagcgttt gccgacgctt tgttttaccg aagcggcgac
     3061 ggtgtcgcgg cggtcgaaca accgctgcat gcattgcgga acactctcga gatgccggag
     3121 ccggcggacg atttgttcat cgctcagcgt tcccagaaga ccggcgaaat aaagggccag
     3181 cacctgtccg gcgaccatct gggcataaaa ggctttggtg gaagcgaccg acatttcaat
     3241 gtcccgtccg tcgctggtat aaaagacgcc gtgcgacgag gcggtgatgt cggactggcg
     3301 gcggttgacg atggagagaa tctgcgcgcc gcgctcccgg gccatggcca cggcccggtt
     3361 ggtgtcggtg gtggttccgg attgtgtaat ggggatgacc agcgtatccg acagatcctc
     3421 cctgaggccg aagccgctca gctcggatgc aacgcgcgac tggatgttga ggcggctgcc
     3481 tttcaggcaa tgggccagcg catccgccac cgcctggccc gccaccgccg ccgttccgtg
     3541 gccgatgaca cagatattct gcgtcttgcc gctttgcagg ccgtttcgaa ccgaagcggg
     3601 aacgatgtcc tcgcccagat tgaacaggac ctgcggcggc gccgaaccgg cgtcgatgat
     3661 ccggtattta ccccgcaatg ttctccggac cgacaggccg gattctgaaa tttctttgag
     3721 aaagaaatga gggtgatggc cgcggtcaat gtcccgcgtc gtgatttccg cggtttgcag
     3781 attttgatct tccggacgaa tcggggtccc gtcatagaaa caggcggcaa tgcccttgct
     3841 gcctccgccc cggtcctgat ccaggatgaa cagttgtccg ccggccgtgc cgctgcccgc
     3901 gtcgccgttc atcctgatga agcggggcgc aacttccacc agaccgtaca gctccgaaga
     3961 aaccatatac gcgtcttcgc tcacgccgac gtaaatcgac tggccgctgc cctggagcgc
     4021 cagaaacatc ttgccgggct ccagattgct ttgcatggca atggcgtgcg acccctcgaa
     4081 atcctttacg gccagtcgaa acgcgtccgt cagatttctc cccgccttca gatatttttc
     4141 gatttgcagg ggaatgattt tggtatccgt cgagacgtcc ggtgcaatct cttccccgtc
     4201 cttttccaaa tccctgcgga gcgccggata attgtcgatg tccccgttca agaccacgct
     4261 gatgtgcgct tccaatcccg gatacgcggg gaaaacaggc ggctccggac ggacggcggc
     4321 attgttgacc ggatgacaat tttcctcggt gatggagcct acggaagccc atcgggtgtg
     4381 ggcaatggcg gattcgtcca tcgacgcctg ttcggcaaac aatcgaagaa tgccgtcccg
     4441 acggatcact tgccgcaggt cggcgacatt gcggcccagt tcgccgacca cggaaaaggt
     4501 tttgtatgta aacgcaacgt gaattccgcc gccatgacct ccggggccgg ttgaaatgaa
     4561 aatggaccgg ttcaacagat cgcctttttg cgttcggcgc cggtaatcct ctccccatcc
     4621 ggtttcttcg atccgtcgca cgagctcctc gatggcgctt tcatttttca gcacgaaggc
     4681 cagctggatg cccgccgagt cccttccccg gacttccagg cggtccagcg cgtttaacag
     4741 cagattcagc ttccggtatt tccgaaaggc ggccggttga agcgaagaac atttttccgc
     4801 tccggagagc gagaggactt tttgcagatt ggtcagaatg tcgcaatcca gcatccagca
     4861 gatatcttta aacagcagca gccggcgatg gatggtttcg aggtcggcgg agctgattcc
     4921 cgcagcccgt tcttccagaa ttttctcttc atcggccaga aaggctttca ttccggctgt
     4981 catgcgggtc aaattcacgg cccgttcttg gtgaaaaaaa agatattcct ggacattctc
     5041 ccgcttcagc tccttgacgc agtttgtgag cgcccggatg gtttcccggc catccagata
     5101 atccgccgga tcggtttgcc cgttgaagat tttttccaag ccgttctttt ttgctttttc
     5161 ccatagagcc cgaagggctt ggtccgatga ggttccggac tccttttgcg cagaccggca
     5221 ggacatcaga ccggcgaaac cgcaattcaa tcttccgggg aggagagggg caaagatgat
     5281 ggcaggtccg tgaaccgtag ccggccgaat gccgaaaaaa atccggcaat gtgttacaaa
     5341 aaagaaccat ttttttaaaa tgtaagacaa ttttgtatgg aacattattt cctttaggtc
     5401 tcattctcgg tctatatttt taatggtctg ttacccaaag atatcctttt cgcttgcctt
     5461 aagagcgttt tgtataaatc taaggctcgc gaaaggcttt gacaaaactc gccttcggct
     5521 caaacagttg tcaaaggctg atctttcgct tcgccaagat ttattggaca aaactctctc
     5581 aatggcccct caaagggata tctttgggca acatttattc cttcctgtgc ttcctatggt
     5641 tctgctcccc cttttcacgt gcttcctccg aaggcggtca cgcaaaaacc attcctgaat
     5701 tttcccggga ggttttcttt gcctggcggc gggtatggaa tgggtggaaa ccggttgcga
     5761 cctttggttg caaacaggat tgcgggggga ctgaagagcc tgccgggagc ttcaggagaa
     5821 agcattcttt ggggaggatc gcttttgatg cgggcgggag gggctccggc gccgatggtc
     5881 aggaaagaag atctttttat tcgccgtcaa ggcccagttt ggtcatttta tcggtaaagg
     5941 tgttgcgatg aatgcccaga aaagccgcag ccgtgctttt gatgttgccg gagcgcctca
     6001 gggcgatttt gaaaaggccc gtttcgacca tcgccatgac ttccgcatga agtttgcttt
     6061 tgccgttgtc ggagcccgac aggatcgttg ccagatcgtc cagctttttt tccaggattt
     6121 cttcgaccgc cttgttgagg gtttcctgaa cgttcatccc cgtggccggt tttggctgct
     6181 tttttgcttt catgatcatg caactcctgc gttaaaaaat gtctttttca tcaaggggct
     6241 cccttcggaa ttccggatgc cccgatcgat ggacggagag cgtcaggccg ccagccaccg
     6301 ggtcagaaga agggcgacca gaatgaccag ggagacccac gtcaatacga tgagcgatgt
     6361 ccgttctctg ggggcctcga tgtaactttt gacttccgcc tccacttttt ccccgcccag
     6421 cttttccgca agaaacttaa gcagcagcgt gtggtcctgc agcagattgg agaagatgta
     6481 aaaccctttg gtcgtcgtca gcagaaagta ggccttgttg cggagaagca ccgtgcccag
     6541 gtgcgtgatt tccgcccagg caaaattttt cgtccggaaa aacttgcgga tttgcaaacc
     6601 gttttctgaa gttcgaaatt ccctttccga aacttcaacg gcaacgacaa gcgcgataat
     6661 gaataaaacg cccgtcagga ttctttccca caactcgccc atcgccattc ccgtcgcgaa
     6721 cagcgcaaac agaaggaccg tcaccgcgac gaaaggaaaa cgaaaggttt ttttgatttt
     6781 ataaacggac acgcgctgtc tccgaattaa tttgcggttt ttctgacgaa ggcgccgctt
     6841 ttgccgccgg atttttccag aagcagaata tcgccgatga ccatttcccg gtccacggct
     6901 ttgcacatgt catagatggt cagtgcggcg acgctcacgg cggtcagcgc ttccatttcc
     6961 acgccggttt gtccgaccgt ctgcgcctgc gattcaatga tcacccgcga ttttttccgg
     7021 tcgatgacaa aagcgatgtc cacgccggtc agccccaggg gatgacacag cggaatgagg
     7081 tcgccggttt ttttggccgc cagaatcccc gcgagtttgg cggccgtcag cacgttgccc
     7141 ttggccatcc tgccctccgc cagaagcttc agcgtggcgc ttttcatggt cacctgaccg
     7201 cgggctttcg cttttcgggc ggtctgggct ttgtccgtca catccaccat ttgaacctgg
     7261 cctgcatcgt cgatatggga taatttcttc atggcggcaa aggacttacc cgtggttgat
     7321 gttttacgga tccaatcagt ttcagcgaac cctagcacag cgccttgacc gagtcaagaa
     7381 tgacgaaaag aatttttaac aaattaaaat cttgatataa atgaatgaat tagctagtat
     7441 ttgccccgcg aattcttccc cggagaaaaa agtgttcgtc aaagtgcaga gcatgagcgt
     7501 gatcggcatg gaatcctatc ctgtgacggt ggaagtggat gtctcccagg gcctgccgca
     7561 gtttgccacg gtcggtctgc cggacgcttc ggtcaaggaa agccgggacc ggatcaaggc
     7621 cgccatcaaa aattccggct atgctttccc gcgcgatcat gtgacggtga atctggcgcc
     7681 cgcggatatc cgcaaagagg ggaccggctt tgatctgccg atcgcggtgg gcattctggc
     7741 cgcggaggcg ttgatcgggg aagccgccct ggacggatgc ctttttatcg gggagctgtc
     7801 gctggacggc agcatcaagg gagtgcgggg tgttctcccc gccgctttca aagcccggga
     7861 gtcggggatt cgttccattt ttgttcccga agaaaacgcc gcggaagcgg ccatggtgga
     7921 gggcgtctgc gtgtatggcg ttcggtcgct tcccgacgtt gtcgaattcc tgggcgaccg
     7981 gaaaaccatc gagccgcttt gcgtgaatgc cgatgacctg tttcagatca accggcgcta
     8041 tgacatcgac ttttccgaaa tcaaaggcca gaagctggcg ctccgggcgc tggagatcgc
     8101 cgcggcagga tctcacaatc tcctgatgat cggccctccg ggctccggca aaagcatgct
     8161 ggcccgaagg ctgcccacga ttctgcccga tctttccttt gaagaagcca tcgaggtcac
     8221 caaggttttt tccgtggcgg gcctcctgag tgccggggaa acgctgattg ccgcccgacc
     8281 ctttcgcgcg ccgcatcata ccatttccga cgttgggctg gtcggcggcg gtcagatgcc
     8341 gcatcccgga gaaatatcgc tggcccatct gggcgtgctc tttctggatg aactgccgga
     8401 gttcaagcgt aacgcgctgg aggcgctccg ccagccgctg gaagatggga cgattacgat
     8461 tacccgttcg gccgtgacga cggcctttcc ggcgaaattc atgctggtcg ccgccatgaa
     8521 tccctgcccg tgcggttatt tcggggaccg ggtccgccgc tgccgctgtt cccctcagca
     8581 aatccggcag tatcaggcaa aaatatccgg tcctttgctg gaccgcatcg acctgcacgt
     8641 cgaagtgccg tccgtccggt accgggcgtt gacagggaaa gagaacaatg aatcctccgc
     8701 cgaagtcagg aagcgcgtgg tcggggcgcg ccggcgccag caggagcgct tcggcggtgc
     8761 ggccattccg gccaatgccc gcatgacgga aaagcaaatc cggtctttct gctccatcga
     8821 tgaagagtcg catcaactca tggaaatggc cgtcgaaaag ctgggccttt ccgcccgcgc
     8881 catgaaccgc atcctgaaag tgtcgcgcac catcgccgat ctggacggcg ccgaaaagat
     8941 cggagccgcg catgttgccg aggcgattca gtatcgaagc cttgatcgga acatcgttta
     9001 gtgctatgaa aaaagcacag ccggcccgga atggccggaa tctttttgtc aagaggtttt
     9061 tatgaaaata aaaattatca aagcgctacc caagtacctg aagcccaaac cggatgaaaa
     9121 aaaactgggg ttcggccagc attttaccga ccatatgttt accatgaagt acagggaggg
     9181 agacggttgg tatgacccgg tcatcgaacc gtaccattac ctgcaactgg atcccaccgc
     9241 catgtgcctt cattacgggc aggaaatctt tgaaggattg aaggcctacc gcgggaccaa
     9301 cgatgaaatt taccttttcc gtccgctgga gaatattcgc agaatgaatg tgtccgccga
     9361 aaggttgtgc atgccgccca ttgacgaaac ggtgtttttg gacgcgctca aacaattcgt
     9421 tgtgctggaa aaagaatgga ttccccggga aaaaggagcc tccctgtata tccggccgac
     9481 gatgattgcc acggaaccgg ccctgggcgt gcatccggcg agcgaatatc ttttcttcat
     9541 catctgcggg ccggtgggcg cttactacgc gcacggcttc gggccgacca aaatttatgt
     9601 gtccgacgac tgcgtgcggg ccgcgccggg cggaacgggg catgtcaagg cggcggggaa
     9661 ttacgccgca agcctctacg cgagccgcat cgccagcgcg atgggctata cgcaggtgct
     9721 ctggctggac gcgagggaaa accggtatgt ggaagaagtg ggcaccagca atattttctt
     9781 catgatcggc gaccggttga ttacgccgcc gctgggaggg acgatccttc cgggagtgac
     9841 gcgtgattcc gttctcaaac tggccgcgca cctgggtgtg aaggcggagg aaagcaggat
     9901 tgccattacg gaagtgatct ccgccatcga agagggaacg ctcagggatg cgtttgccac
     9961 cggaacggct gccgttattt cgccggtcgg acagattttt tatcacaatc gggaatacct
    10021 cataaacgat ggaaaaaccg gggaactggc cgaaaaactc tatcatgaaa tccttcagat
    10081 tcagtacgga ttgaaagaag acccgtttgg ctggagaatg cggatcgcat gaggatggca
    10141 cgaaaaaaac aaaatttttt tagccgttta agcatttttt gcccaaacgg gaaaccgtat
    10201 tccacaaagt tgtggaaagg cctgtggaca accatgtgga taaacccggc aagtccgcat
    10261 tgcttaggct tttagaccgg attgcttaag gtttgggcaa ttaataatgt aagtaatatt
    10321 aaatagttac ttatatatcc ttgaatcaca acgacttatg gcccccgcag gtccgagata
    10381 ttaaccgcac tgaaaaaaat gtgccatctt tgagcgggtt ttctctattt tgggaaataa
    10441 aaaaagatga aggtaaatct gaataaaatt gagttgttta tgccggccgc cctgctcgcg
    10501 gtttgcgcgg cattgttttt aggggttgct tgccccgctg cttcgccggc aggacccgcc
    10561 cctgccctgg agggagtgat tgaccgcctg cagcagattt atgaaaaaac gcaggacttt
    10621 caggccggtt ttattcagga aaccacggtg aaatcgatcc ggaaaacgga cacggaggcg
    10681 ggaacggtct attttaaaaa tcccaggcag atgctctggg attataaaaa gccgaagaca
    10741 aaaaagctga tcatcaacgc gcaaaaagcc tggctttacc tgcccgggga gaaaaccgtc
    10801 tatacgcagg aatcgaataa gatttttaaa tccgaggcct taatcaaatt tctgtccggt
    10861 ctggggaagc tgaatcatga ctttacgatt caatacgcct ctcccgcggt ggatttggac
    10921 ggcaactatc tgttgttgct ctacccgcgt gaaaaaggcg catcttattc ctgtctgaaa
    10981 atgacgattg ccaaaaacaa tttttacatt ctccaggtcg gctttgacga cgtcatgggc
    11041 aattccacgc tcctgaaatt ttcagacatg aaaatgaaca gcgggcttcc ttcgaaactg
    11101 ttccagttcc acccaccccc cggcgtcagc attttcaaga tgccgtaatt cagacggttt
    11161 tttagaaggt ttgaaaattc agcttcagcg ctttcaaacg ctccatgaat ttccggtcga
    11221 atgtgacaac aatcgaccct ttgatgtttt tgcaaatcga cgcgattacg gcatcgccga
    11281 agtcgggaac agattccggc caccatgata aaacaagcga ataatcgacg gtgttcacca
    11341 cttcaatgcc tggcatggcg atcagatcgg aaatcatccg ggcgatttcc tttttgggga
    11401 tggaatagac cttctccatc acgaagatga attcggttaa cgtgtgctga tgaacgaaaa
    11461 gaaacacctt gatctgtgcc gcctgttcga aaagatcggc gattatttcc tgctgatcgg
    11521 gattgcggtc cgtcacgaag gagatcaggg cattggtgtc gatgatatat ttcttcaccg
    11581 gtatttctcc aggcgctttt cacgcgccag ttcaatatcc gcggcgatgt ctccttttcc
    11641 cactttcagc gcgttgcgat gtttcaaaaa ttgcagccgg gaaggataaa caaccacttt
    11701 gccgttttcc agcttccact ccagggcgtc ttttacggaa aggccaagat tatcacggat
    11761 ggccttgggg atagtggttt gatatttcga ggtaattacg gattgcatgt catgcctcct
    11821 gttcctgtgt ttccttacaa tatgcttata acgtaaggaa tagggaaaag tcaagttgac
    11881 gtattatccc ggtttggcgg ctgttatcca gagaaaggtt tcgccgccga tctgacggta
    11941 acgtgacgat tcgataacaa agccgataga ttttaaaatc gcggccaggt tgccgggcgt
    12001 cgtgtggctg tagaacaatt tttcgcccat gaattccttg tatccattga agatttccgc
    12061 cccggtgtat tcctttgtgg cgtaggtaaa aagcagcttg cctgcgggtt tcatccaccg
    12121 gaaaatcttt tggaaaatgc ctccgtgttt ttcatgctcg atgtgaaaca gacagtaaat
    12181 ggcggtgacg gcgtcaaatt gacgatcggg aaattccaca ttgcggatgt cggcttccac
    12241 ggtttccatg ttcggctggt aatgacgggc cagttcaagc attcgggaag aaaaatcgac
    12301 gcccgtcacc cgccagcccc gcgtgatgaa gaaggccggg aaaggctcgc ccgcgccgca
    12361 gcccagatcc aggagccttc cgggccgctg cggcagcctt cgggaaaaat cgtcgatgac
    12421 ttcggacatg tcgaacaagc cgcggttttg ttcatacgtg tcggcgaagc gatcgtaaat
    12481 ctgtcgcaga tcaccggaca tacattcacc cggttttatc aaagggattt tcacgattga
    12541 aatccggcgt cagggctctt tcatcgactt cctgaaggaa cagattttca aaccccaggg
    12601 aaagggcaaa ttccacaatt tcatgatatt ctgcggctgt gatccggcgg ctcagcaacg
    12661 gatgcgatcg cactttcggg atgggcgtgt attgcgacat gaggctgatc ggaatacgcg
    12721 cggagatctc gccgcgcaaa agcctcagcg cctccttcga gttttccaga cgccccggca
    12781 ggagcaggtg gcggataatg atcccgcgga ccgcaacgcc gttttcgatg tccagatcat
    12841 ctcccacctg ccggaccatt tcccgaaggg aggcgagggc gaaacgcggg tactgcgggg
    12901 cgttggagaa ggcgagcgag tcttcttcca gaccgtattt gaaatccggc agatagatat
    12961 cgaccatgcc gtccagcatc cggatcacgt ccggattttc atagccgccg cagttataga
    13021 ccagcgggac ggtgagcccc tgggcccgcg ccagacagag ggcttccatc accagaggcg
    13081 tctggggcgt gggggttacc ggctcgacat tgtggcagcc gcttttctgg agatcgatca
    13141 tgatccgggc cagttcgatg acggtcaaac ggcggccttg cgccctgtgc gaaatttgat
    13201 agttctggca atagacacat cccagattgc aggaagaaag gaaaatggtg cctgcgccgc
    13261 ctgttcccga aagcggcggc tcctctccgt gatggggcag agcgcagtcc atgacgatgt
    13321 cggaaggcag gcggcagtag cccggttcgc cttttgtccg gtcaacatgg caggcgcgcg
    13381 gacaaagggc gcaggatcgg tacatgtcaa ggagttcggg aatgatcata aacacaagaa
    13441 tagcgcaggc agcagacccc ggattacagc cgacggcctg ctgcctgaaa cagcaaaaga
    13501 aaaaacgttt gcggaaacag tccggccgtt cgcgttgccg ggagagaggc cgcgacggct
    13561 ccttgccttg ttttgattac tcgccggtga agttcggttc gcgcttctgc aggaaagcgg
    13621 tgatgccctc cacggcgtcc ttgctggcgg ccagtttatt ggaccactcg atgtccagct
    13681 tcaggccttc attaaagccc ttttccatgc cgacgctgat gccttccaga acggcggcca
    13741 cggccagcgg cgggcgtttg gtcagcgcct gggcaaatgc cgtcgcctcc ttcatcaaat
    13801 cggcggccgg aacgacttta tcgaccagtc ccacggccag cgcttcggtg ggcggcaggc
    13861 gtttgctgaa gagaatcaac tccagcgcct tggattttcc cagaagcctg ggcagccgct
    13921 gaatgccgcc ccagccgggc gtgatgccca gattcacttc cggcagtccg atcagggctt
    13981 tgggattgtc cgtcatgatg cggaaatggc aggcgatcgc cgtttcgcat ccgccgccca
    14041 gggcgtgccc gttgatcgcc gcgatgtaag gcttggtgga gcgcgagatg gcgttcatga
    14101 tgtcgttgcc gttggggccg cgggtgatgt tggccacgtc ggagacgtcc atcccggcgg
    14161 aaaagccttt ttcgcccgcg ccggtgatga tgacggcccg tgtttccttg cttttttcaa
    14221 tgttcgtgac cgcgtcgaag aattcctcgc gaatgcccag attcacggaa ttcatcggcg
    14281 gacgattcaa aacgatcgtc gtcacaaaat tctcggttgt tacaataatg ttcttgtatt
    14341 ccatcttctt tctcctttca gatttaagag caagggattt gcatcccctg ttccgtgttt
    14401 atccttaggt ttgtagggaa cggtcgcgac cgttccctac ctaattttcc tggtgcacca
    14461 gccgcatgcc cggatacggc acctcaaagc agggaacgtt cgtgccgccc aggcttccga
    14521 taaaagccgt gcggcggttt tttccgccga aacaaatatt ggccgggcgg gaaagcacga
    14581 tgccctgctg gtcttcggca tagaggttca gggcgccctg cggatcaatg tagcctacgg
    14641 cattggcgac aggaaaggcg acccagagat tgccggcttc atcaaacgcg atgccgtcgg
    14701 ggaacccgaa cttgcccaga aattcgggac cgtaaacctc tgccggtcct accgtgtcgt
    14761 cggcatgaat cctgtagcgc aggacgcggc gtttcatggt ttccgccaca taaacatatt
    14821 gttcctccgc gtccaccgcg acgccgttgg caaaacaaat tccggaggcg acgatttgag
    14881 gcgccctgcc tttcgcgatc accaccaggc atccgtcggg gatgggcgcc cttaaggatg
    14941 cgtcgatatc cgggttgtcc gtggagttgg aaacccagag acggcccgca aaatccagga
    15001 agggaaaatt gggcgtgaac attcttttgc cctgagcctc tgtcatcagg accgtgtgcc
    15061 gaccatcggg ggaaagagac tggacttcgc cgttgccgat gttggcgaca atgcagttgc
    15121 cttccgtatc catgcagatg ccgttgggca ggccgcccag tcggccgtaa aaagctgttc
    15181 ggccgtcggg atcgatccgc gaacagcgtc catggccgtc cgccgtgcgg acgctgccgt
    15241 catccagcgc catgacgcct tccggccgcg tcagtccgca gccgatggtt ctcatctgct
    15301 gaagttcaat tttcatgagt cccctccgga tttgctccga cgtttcgctg cggagctttc
    15361 aacaccgtct gctctccttt gctgaaggcg acgcaggaaa gcgggatgcc ccgatggacg
    15421 gcctccaggc attgattgca ataaacgcaa cgcggttttt tcccgggatc ggcttcccag
    15481 ctccggatca gacccggctc cgagatcagc ggccggcaaa gcgagatgaa atccgcctcc
    15541 ccgccgcgaa tcatttcctc catcatggcg atggatttca agccgcccac ggcaatgacg
    15601 ggagtatccg tctttttcct gatttccccc gtccatgcgc ggaaatagcc ttcccgcgac
    15661 ggtatgttga ttttggttct gtattccgtg ccttcgtatt tttctccccg aacgcccccg
    15721 ctgatctcca gcgcatcata ccctgtctcg gcaagcatca gcgccgcttt catcccctcg
    15781 gaaaatgtca ggccgccttt gaagccgtcc tcaaccccca gcttgatccc gacgggatag
    15841 tccgggccgg catgatcgcg gacggacgcg taaacttccc tgtgcagccg caggcggttt
    15901 tccagcgagc cgccccacgc atcctcccgc cggttggtaa agggcgagag gaactggctg
    15961 aaaagatacc cgtgcgcgcc atgaatctgg acggcgtcaa accccgcctc gcgcgcccgg
    16021 cgcgccgccc ggccgaaggc ctgaacgaca tcctcgattt cccgggtcgt catttcgcgg
    16081 taattccagc ggtaaaaggg atcgtcggcg atcaccgagg gaccgatggg caattggcct
    16141 ctggttttga cgaagcgcgc ctcgcggccg ccgtgataaa gctgtacggc aatttttgcc
    16201 ccccgctgat gagcggcccg ggcaagcttt tgcaagccgg ggatgcagga atcttcggca
    16261 atcgaattca tgaatgacga aacctgaccg gagggatgaa cgtacgtaat ggccgtgacg
    16321 atcaggccga cgccgccctc ggcgaggccg gcaatcaagt tgatctggcg gtccgtcacc
    16381 tgtccggtgg gatccgacat gccgtcatac gtggccgacc ggacaaaccg gttgcgcagg
    16441 gtcatgtttt tcagccgggc ggattcaaac agaatgctca ttgctaacct tcggccagtt
    16501 ccgttgttta ctcacattgg atgtgtgcgg gcaccctatc acgatttggc ggtttcaggc
    16561 aatggtttgt gaaaggcggg gtcattcttt tctgccggaa caaggatttg tgcccgcaaa
    16621 tcaggctttt gaccctgtga tggaatgtga ttgacaggtt tggatcttct gtctatactc
    16681 cgcgccatcc tgacagggaa ataaccgaaa gcaaaagggt tttctatgtt taatcgcatt
    16741 gtcagcattg gttttctgat ttttatctcc ttgacgtcta ttccgctgtt tttgacggct
    16801 ttgctcattc gtgtggtaac ttaccccttt gaccggcggt tgcggctttt gcatttgttt
    16861 acctgctttt gggcttcgat ttatacctgg acgatgccgg cctggcggat tcgcatcgaa
    16921 ggccgtgaca acgtccggaa agacgcggcg tacatggtcg tttccaatca ccagtcgcag
    16981 ctggatattc tgatcgcctt ccggcttttc ttccattaca aatgggtgtc gaagattgaa
    17041 atattccgtg tgccgctgat cggctggaat atgatgctga accgctatat tcaactgaaa
    17101 cgcggcgaca agcagagcat cgaacagatg ctcagccaat gcgccatgca cctggacgag
    17161 ggaagctcca ttttcatgtt tccggaaggc acccggtctc atgacggcga agtcaaggcc
    17221 ttcaaaccgg gagctttcca actggcgcac cagaagaagg tgcccattct gccgatcgtg
    17281 atcagcggaa ccaacaaggc ccttcccaaa tacagcatga attttaccgg tgtgcagaaa
    17341 atttacatta aaatatttga agagattccg tatgacgctt ttgcgcatct ttcagttgga
    17401 gagacggctg aaatggtgcg cggtttcatc agccggcaac tgcggttgat gaatgaatat
    17461 acgctgggca atgtctgacc gtatcttccg gagagcccct ttacattttc agcagggcgg
    17521 cgccgatttt ttcccgacgg atttgcaggc gtcttttttg aagcatttcc agaaatgcca
    17581 tgtctttttg cagcaggccg aagcgttcgg gcggctgaag gtggagttgt tcgggccacc
    17641 gggcgatgtc ggcgtgaaac tccgccgcga agcgggcaaa ggcaatgccg gcggatgcgg
    17701 tggatccgtc gccccggtca agctttccgg ctgcttcata agcgatgctc cgcagggctt
    17761 gccacagggc ctgcagcgcg ccccattcgc cctgcagggt gcggtccgtg gaatgccccc
    17821 ggagagcgga attcaggagg tccagttgtg cggccatgcc gcccagaacg ggtcccatca
    17881 tcaccacgtc ttccgtttct cccaaaggga cgacgatgtc ctcccaggcg tttccctccc
    17941 ggccgaggac ggaggccttc gggataaggc agccgttcag ttgaatgccg ccgtgcggtg
    18001 aaggctttaa aaagttcagg ggcatcggcg gctctacaaa gaggccctgc gcgccgcgcg
    18061 ggaccatgaa cgcggtaaaa ccttttcgcg ccgacgtatc gtcggttacg gcgacaacca
    18121 caaacaaatc ggcaatcggt ccgttggtca ggtgggtttt ttcgccggtg aggaggtagg
    18181 attccccctg ctgttgcgct ttcgtggcca gtgctttcgg agaagcgccg cgtcccggtt
    18241 cggacacggc aaagctgatg gcgcagtttc ccagagctgc ggcgcgcaga tattgccggc
    18301 attgctccgt cgtgccgaat gttcccacaa cggagcgggc gatgatctgc tggaagatcc
    18361 aggacattcc cagacccaga ttcagtccgc tgcggacaaa cgcttctccg gaccgcaaaa
    18421 ggtcaagata gccgccgccg tcaccgccgt attcttccgg aaggccgccc cggaacagcc
    18481 cggcctttcc cattttccgc cagaggtctg aaggaaattc ctgctccgtc tgcaaatcag
    18541 cgcggcaggc gacgtgccgc gcggcgaaat ccgcggccag cgccggcatg tcgaaaggct
    18601 tgtccggttg tttcatatga ccagttcctc gatgaacgtg aacaactggt tgacgttgcg
    18661 gcaggcgcgg acttcatgac agtggcgcag gtaggtccgg atctcgctgt cgcccgtgcc
    18721 ccacagtttc tccggttcgg gattaagcca gatgacccgt cggcagcgct cccgcatggc
    18781 gcccagaatc gcgtcttccg gattcatgta attcgaccgt ccgtcgccga cgatgatgag
    18841 cgtcgtcttc ttcgtcagaa gatccatgta gtctctttta aaatttctga gcgtctcgcc
    18901 gtaatcggtt ggcgccgagt agtgcacgtc cgcttcttcc atgacgatgc ggatggcttc
    18961 ggagatatcc tgctcctcaa agatgccggt gacgttcgtc aactggtcga tgaagacaaa
    19021 actgttgacc ttatcgaagc agtcctgaag agaataaacc agattgagca tgaagcgggc
    19081 cgcggcccag acggatccgg acacgtcgca tagggtgacg atccggcttt tgcgcaggct
    19141 tttccggcgg tattggatgt cgagggggac gccgtcgtat ttggccgacg cccgcagcgt
    19201 ttttttcacg tccagatttc cccggtcgcg gacggcgaag cgccggctga cgatgttttt
    19261 cagtttgcgc accagccggt cgatggcttc gcgcatttcc tccacttcct cgcgggtgaa
    19321 ggacgaaaaa gccttttcac cgaggtcgcg cacgcgctgc tcgtgcgttt ttactttttt
    19381 ggcgccgccg tcgcccgcct gcggctcgta aaccagaatg gaccgcgcgg cctccactcg
    19441 ctcttcaaaa tgagccgcaa ggttatgacg gtccgccggc gtcatgttca attcattgtt
    19501 ttccagaagc gcggcggcgg cgtttgccgc ggcattgatc tgcagcagaa cgttgaagcg
    19561 gctggcgagc ggtccgagat tgaaccgtgc gggcgctgcc gcgtcatccg attccaaatg
    19621 gatccggcgc atttccgcca gcagggccat gggatttccg gccagaaaat ccaccacggc
    19681 atcatacatc ggattgtcat gcggcttttt cctgagcagg tccgtcgctt cccgaatgcg
    19741 gtcgatctgg gaggctgcct gctgcatgtc ctccgcgttg acgcgcattt cgtgaaaaaa
    19801 cagatggtag aggcgttcaa agtgcttcag ttcccgcagg cttttggcaa aattggcccg
    19861 cagcagggcc ttgaactgca gttcatcggt cgggtcgatc agctcgagct gcttgagcgt
    19921 gtcgaggact tcggaggtgg agacgcgcag ccccgcggcc cggcagcagg aaacaaattg
    19981 cccaatcagt ttgaccataa atcaccacgg cgggattccg gaggcgcacc cgcaaaagac
    20041 ggctgccctg ccggtcgggt ctttacgggc tgttgcccga atatccttaa tcttttccgg
    20101 gacgggaagc gtgatgaagg cttcatccgg ctccggcgac gacccggtcg atgtttttcc
    20161 gcactttttc cagatccgcc tggtatttgc agatgacgtt gagggtttcc gagatgactt
    20221 ccggcgtcag ttcggtaatc tgcagcgcca gaagcgattg cgcccagtcc agggtttcgg
    20281 aaatactggg gctttttttc agatccaggg tgcggatttt gcggatcgaa tccaccagga
    20341 cggcgcagag tctttcctcg atgtccggaa ttttcagacg gacaatcgag atttccgtgt
    20401 cgcgcgccgg ataatcgatg tagagatgca cgcagcggcg cttgagggcg tcgctcatgt
    20461 cgcgatagtt gttggacgtg agcaggacaa aggggatccg gacggccgtg cgtgtgccca
    20521 gctccggaat ggaaacctga aaatcactga gcagttccag cagaaaggcc tcgaactccg
    20581 gatcggactt gtccacttca tcgagcagca gcacaacagg cttcgccgaa ctgatcgcct
    20641 gcagaagcgg acgcggctgg atgaagcggt cggaaaagaa agcgtcttcc tgagcggcga
    20701 tggcatcgac cgcttcggca agcgttgccg tggcgccgat gatgtcgttg atccgatcct
    20761 tgaccatctg tgtgtagagc aactgcttgg cgtattccca ttcatagagg gctttggcct
    20821 cgtccaggcc ttcgtagcac tggagacgga tcaactcccg ccccagactg cgcgccaccg
    20881 ccttggccag ctccgttttg ccgacgcccg ccggcccttc gatcaaaacg ggcttctgag
    20941 tcgcctgcgc cagaaaaata accgtggcga tttcccgaga cgggatgtag cctgcttcga
    21001 tcaacctttg ttcgacatct gagaccgttt tataggacat atccgacctt tctgccgaag
    21061 ctcttataac aaacattccc cgcggtcaag aataagcggc ctgctcagca aaggatatcg
    21121 ttcgggcaac agactgtaag ggcaggacag gaatggattg tccgggcgcg ggctgttttg
    21181 tctgttgtcg tcaaggatga ttgaaaggct gattcgccct gaaggatgac cggcaaatga
    21241 gcgtttgact tcctgagatg gtttgtggta gttaccctgg aatttttttg taaatttgaa
    21301 cgatttcaag atgctgaatt gtaaagggag tccggccgat gacgtgggaa gcaatggcga
    21361 atatctttca gggggtcacg acgctggcgg agtccttttc ccataacccc ataatggcgg
    21421 cagccagaat cggattgatt ttgctcgggt tgctgatgat ttatctggga agaaagggca
    21481 ttctggaagc ccttttgatg attcccatgg ggctgggcat ggccaccgtc aacgcggcca
    21541 tcatgttttt tgatcccctc aacctgcacg gcggcacggg gacgcttttt gtggaggcgc
    21601 aggccggcgc tacggccgat gccgtgaaaa acgctgctga aatcattgcc attttacaga
    21661 tcgactggct tcagcccatc tacaccttta ccttcagcaa cggcctgatc gcctgcctgg
    21721 tgtttatggg gatcggcgcg ctgctggacg tgggctatgt catggcccgc ccgtatcaaa
    21781 gcatgttcat cgccgtgtgc gcggagctgg gaaccatcgt cacctttcct gtggcccagc
    21841 tgttcggcta caccccgcag caggccgcgg cgatttccat cgtgggcggg gccgacgggc
    21901 ccatggttct gttcacctcc cttcggctgg cgccggagct ttttgttccc atcgccatca
    21961 tcgcctacct ttatttaggc tttgcctacg gcggttatcc ctacctgatc aaaatgctgg
    22021 ttcccaaaaa gcttctgggg atcaagcccg cgccgcccaa aaagaagcgc aacatcacct
    22081 ccggcgagaa aatgctgttt gccgtggtcg cctgcgttct gctgtcgttt ctgctgcccg
    22141 tggcggcccc gttgatcttc tcgctgtttt tcggcgtcgt catccgggaa tccggcatta
    22201 aggagttcat tgatcttttg cagggtccca tcctttacgg gtccaccatg tttctgggtc
    22261 tgatgctggg tgttttgtgc gacgccaaca cgatcatgga cccgaaggtt cttcccctgc
    22321 tgcttttggg cgttttgtcg cttttgattt ccggcattgg cggcattctc ggcggataca
    22381 ttctttattt tgtgacgcgc ggaaagttca atccggcgat cggcatcgcc ggggtgagct
    22441 gcacgccgac cacgtccaag gtggcgcaga aggtggtttc caaagtcaac cggggcgcca
    22501 ttattttgcc cgacgcgctg ggcgcgaatg tcagcggcgt gatcacgacg gcgattattg
    22561 ccggtatttt ctgcagcctg ctggcgaaat aaaaggagaa gcctcatgag cggatggccc
    22621 gaagcgttgc tgacctatgg actgacgatc gtcattgctt tcggcgttgc cggcatgatt
    22681 cacctgatgg tcgttttgct ggaaaaattt tcccctaaaa aagacaacta gcggcgcgca
    22741 aacgccgtct gcctgccgaa tattccttcc ttgaaattcg tccggtattc tgatttattg
    22801 gcctctggcg aggtggatct gtccatgaca acaaaatccc catccacgga aaaaagcgat
    22861 tcctgggttt catcggcgct gaaagtcaat ctggagcgca cggcggcgga agtccggatt
    22921 cctccgcagt acgcgccgct cttgcagatg gtcgaggacc actacggcct gcaaaaaaaa
    22981 acgcgcgagc ttttgacgga gctcaatcac ccctatgtca actgggattt tgtccttcgg
    23041 gaactcaagt ccatctccat cggcaatttt cacctctaca gccgtcgggc ggacgggctt
    23101 gccggtttat ggatcctgtt gaccatttat ttcgacatcc tgaaatccac agccagcgaa
    23161 gacgtgaaag acagcgcgct gcactacctg tttgactacg cggacacggt gatcctgcac
    23221 agcgaagaat ttctggagag gaatctatcg ctgtttcccg ggatgatcga atccctgacg
    23281 gcgctggcgg acggcgaatc ggctctgttt aaaaaatgct cttcctatct gaaaaggatc
    23341 ctccggacgg cggccgacag gaacgtggat ctggccacac cggcattcga tgcgctgctc
    23401 tacaaaatgt tccgcgcgac ctacgctttc tggctgaatc agcccgatcc agctttatgg
    23461 ctggtggccg aacgcaaggc cgaggcgccg ccgaacgaaa ccccttatct ggaattgatc
    23521 cggccgcttt ctcatcaccg gttccgcaag ctgatgctgg agctcgaagc gcttcgtccc
    23581 cgggacggcg aaaaagccgg ggcgcatctc cgggactttt tggcgcttcc ggattatttc
    23641 cagatcctcg acacctacct ccgggtcgcc tccgcgctgg aaaaatccga agcctatgcg
    23701 agccgtcacc ttgtgaagct ggatttcctc ttcggcatca tgacggcgcc gggtctgcag
    23761 gacctccacg tcagcgccat gcgggaaatc aatgcgtcgc tgaaacttgt ttttcaggag
    23821 gaaaagaaag agaacctgga tatttttgta aggaaaattt tcggtttcct gaaaaaaaac
    23881 acatcgcgga gtgaattccg cagcgcgagc atcgactgca ttctcacggc ggcccgggag
    23941 gtttttgcac aaaacgacca ccccctggtg gaaacgttca tggacgaact gatcgcctac
    24001 ggatttgaat atccggagat tcaaggatcg acggccgaat ggcaggtgcg ggtcaatccc
    24061 gaacacatcc ggacgatccg cgcctggctg gaaatcatcg ccctgaagcc gcgctggacg
    24121 aaacggctcg tttccgcgct gattattcat ctgaaaatcg gcggcatttt catccgggat
    24181 acggacctga ttcagcgcga catttcccgt ttgctcaacg cggacattgc tccggcctac
    24241 agtctgatca agcagctttt gcggttgttt cccgtttact tcagcgaaat cggcgccgaa
    24301 ggagaactca gggacctgac gacccgtgtg gatgagctgt cctgccgcaa cgaccgcctg
    24361 atcgattttt tccgcaagca gtcccacgtc gagagcaaca gtctgctggt cgaatttatc
    24421 gaggatattt tccgcttctg ggtttccggg gagaagcgaa ccatccgcaa acatgtcccc
    24481 gaggaaatct atgatcaggt ggcaaacgag ggtctttatt ttgacggcat gcatcaggcg
    24541 ttgacccgcc tctttgaggg catcggcaat caccccggga aattcctgga atgggatccg
    24601 gagaaaattg ccggggaaat atcacggatt ccgaatattt ccgagacgga ccgggagcgc
    24661 gcgtcgctca tgattcgcat ttaccagttg atttacaaga aataccatcc ccggtactat
    24721 gatctgcttg gcgatttgga gtccgccaat gtttttaccg cgcaggatct tctgtcgctg
    24781 aaaagatcgc tggccgataa aaaccattac cggagtctga cggtcatcct gaaatgcctc
    24841 ggttcgctca aggctcaaat tctttccgaa aagaaaaccc gctcttttga aaacatctac
    24901 tataaaaggc atattgccgc ggggattccg tccatgtatg gaacctacca cgaggaaaaa
    24961 ttcgacgcgc ttgggctttc cctgcgcctg gaaagccttg cgggaatgct ctttgaagag
    25021 ttgattcaat ccctgaatct gcagtttatc accaagcgca ccatcatccg gatccataac
    25081 tatctgtgga cctatctgaa cgcgctggat ctggaaggga tctccaccga aggtctggtg
    25141 gccaaagtcc ggtacatcac cagcgcgctg cccatcaagc agttttccat ggagcagtat
    25201 ctggatattt tccggttcat ctcccggggc attcaggatg tcatccgtga ttactatatc
    25261 gacgcccaca gcgttaattt gccggtgatc atccgtcagc ttcaggcgca gacggatcca
    25321 tccgacggca agcaagacga aacccgtatg gaggaggtca tctatcagca atcggagaat
    25381 tttctccggg gcgtgatcgc gtccgccttc ggtctgcagg cgctggacaa ttttgttcac
    25441 accgtcatca agacgctcaa cgcggagctg gaaaagttca aggacaacaa gcggattgtc
    25501 aatcttctga tggattacaa tccggagctg gccgtgtctc ccctctacag caagaacccg
    25561 aaaacggaca accagattct gctgggcaac aaaggctatt ttctgaagaa gcttgtttcc
    25621 ttcgggttcc aggtgccgcc gggcttcatc atcaccacgg aagtgttccg gggattcgac
    25681 gcggtctatg ggtacaagta cattttccgg gatctggccc ggcgggtcaa ccgggaaatc
    25741 gccgcgctgg agaagaaaac gggccgccgt ttcggagatc ccggcaatcc gctgctcttg
    25801 tcggtgcgaa gcggcgcgac ggtttcgctg cccggcatga tgcggtcttt tttaaacgtc
    25861 ggcatcaacg cttcgattgc cgaaaccctc agcgccagaa aagattttca gtgggcggcc
    25921 tgggattcct accgccgctt ccttcaaacc tgggggatgt tccaggggct gagccgggac
    25981 ttcttcgatg ccatcatgga cggcttcaaa cagaagcatg gcgtgcaaag aaaaattcaa
    26041 tttccgcccg ggctgatgaa gcagattgcc ttggtgtata agcagggaat tctggacagc
    26101 ggcattccgc tgtcggatca tccgctccgg cagcttcgtc atgcggtttt aaaggttttt
    26161 gattcctggt atgccgagca ggcgcgcatc taccgccacc agatgcactt gtccgatgca
    26221 tggggaaccg ccgtcatcgt acaggcgatg gtcttcggca acctccatga acggtccggt
    26281 tcgggtgtga tctttacccg cgatccgaaa agcgcttcct ccgacgtggc cctttacggg
    26341 gatttcatct tcggcgttca gggggacgat atcgtttcgg ggctggtcga aacgtatccc
    26401 atctccgagc gccagcggct tgccgaacag cgcaacacgg gaatttccct ggaagccgac
    26461 tttccggcca tctatgccga actggtcaaa accgcggaaa cgctgattta tgaaaagggc
    26521 ctgaaccacc aggaaataga atttaccttt gaaggcccgc aaaaagagca tttgtttctg
    26581 ctgcagacgc gggacatgga tcagacccag gcaaaaagcc tgcggcgctt taaagagacg
    26641 gacctcctgc acgcctccat gctgggaaca ggaattggcg tcgccggcgg cgccctttgc
    26701 ggccgggccg tttattccga aaaagacatt cagcagtttc gccaggcgga gcccaggacg
    26761 ccgctcattc tggtccgacc cgatacggtt cccgatgatg tcggcgtcct tttgcaggtg
    26821 gatgggctgc tcaccgccaa gggcggagcg acctcccatg ccgccgtaac cattccgcag
    26881 ttgggcaaag tcggtgtcgt ggggctcagc aaactgaagg tctatgaaat ggaaggctat
    26941 gcgacgctgg agggccgcgc catccaaagc ggagatttta tcggcattga cggctggagc
    27001 ggagctctgt acctcgggaa gcatgacctg gaatcagctt cctacgcttc ccttgtcccg
    27061 taaggcgttc cgcgtcaaaa acaacaatct cctccccagg ctttttttac ctgatgcatg
    27121 gagagctttg aaaaactcct ttttcgtcat accgtcgaaa gacggtatcc agaaaagcat
    27181 ttgatatgac tggattccgg atcgcgctgc gcttgtccgg aatgacggtt ttgatggttt
    27241 tgcaccttgc acgatctttg ccgggtggtc ccgtataggc tttccaacaa aaaaccctcg
    27301 ttttcctttg cgggaaaacg agggctttgt ttttgagcat caacagctta ctgaatgttt
    27361 ttgtggatat agacggtcag gtcgcccatc cggttcgtgt agctgccgta ttcgttgtcg
    27421 taccagccga aaatcttggc atggaccacg ggaacctgaa gcattttttc gccaaggctc
    27481 gaaacgaggt ccgccggcag atttggaatg tgcgaaaggt caaccggtat gaaagccgtg
    27541 cgggtgtgat tgaactgccc ttcaatgatg accgccgcgt cttcgccgat cagatcggtg
    27601 gagacattct gctgcatggt gaaacggaca agcgggttgt cctgtgcggc cttcgcgtaa
    27661 atatcattga gctttttggt gtcgaggccg gccgccgcgg ccttgtcgtt gtttctggcc
    27721 tggaacgtcg cgttgagcac gatgagcgaa agagtgttgg tcggcacccg gatggaatcg
    27781 cccatgaagc cgatgttttt aacttccgga ataacttcgg ccagggcctt ggccgcgccg
    27841 gtcgaggtca gaatgatatt gttgaggatg ctgcgcgttt tgcggatatc tttctcgccc
    27901 gccttgggca gcttgtccag aacgctctgc gtattggtca cggcatgaat tgtggacatg
    27961 gacgccgtca ggatggtgct ggtttccttg ttgtccagaa gcggtttgac catgtgcgca
    28021 agccccgttg tggtgcagga tgccgcggaa atcaacaggt gcttcttggg gttgaaggcc
    28081 gtatggttga tgccgtaaat cagcgtcgtg gtatcttccg gcgtcgaaag agccttgttt
    28141 ttgatcttga aaggcgcgga atgaatgacc gctttggcgc cgccgtttaa gtgaccccgg
    28201 atggagcctt ttttgtcgtc cacgggaacg gtcggatctt tgaagctgcc gctgcagtcc
    28261 acgacaatgt ccgccccgta ctggcgccag ggaatattca tgggattgcg ctgttcccgc
    28321 aggaccgtca ccgggatgcc gtcgatgagc atcttgccgt ttttttcgtc cgtaatctga
    28381 atgcagggct gggctttgat gccgtacaga aaccggtgga gcaacccgta ggtcgcgtcg
    28441 gcttcaatca gccgggcgat ggtttccatc cccatgccga tgtcccggcc ctggttgacg
    28501 atgatttctt tgaagtactt tctgttgacg tgatgccaca gggtcagttt accgatgcgt
    28561 cccaggctgt tgatgccgag taccggcaac ctgctttctt ttgcgctgct gttgctcatg
    28621 atgtatttcc tcctttacac cctttcgggc gggaatgatt ttttggcgtg aaacaaagag
    28681 gatcgggtac aggacgacct tctttgtgtc gcggaaggta tgacggtgta aattttgtac
    28741 aaatccgtta tccgttactg tattgaggtc gttttgagaa gaccggaaca cctttacagg
    28801 aaagacccgt caaagtcaag agcgtaatag ataaatataa ttatatcata tgtatacagt
    28861 attttggcct tgtcggaggg ggggccgccg ccagcgtcct ttccaccggc cgggatattc
    28921 tgaccggggt cgagaccgcc ggttcagcag caacagcccc cgctctttgc ggtttgattt
    28981 tgaccggtct gcggtccgca gcagcattcc ccgccgtttc gtcccagcat actgttgatg
    29041 acggcagcgg cgcagccgac gcccgattga acgctgattg cgcccgccgg acaatttctg
    29101 ctgcacgcgc cgcattccat gcaggcgttg cgatcagcga tttgcagacc ttggccgttc
    29161 attttgaata ccccatgagg gcaaacatcc aggcacatgc cgcagccggt gcatttttgc
    29221 gaatccagcg ccagggtggc aacatctttc agataaatca tacttcccat ttttactact
    29281 cccatcgaag tatcaatgac gttatccaga ccatcaggcc gacaatgccg gccgttattt
    29341 ccagaggcag agcaaaccgc atttcctttt ttacgccgga gagcgacgtg taggttgaag
    29401 cgccggtaaa attcatggcc aggtaggccg cgatggccgg aatgatcagg agccaggcca
    29461 ggatttctac cctgccggcc catgtcccga catcaacgcc tcttgccatc atcagtgcga
    29521 gggctgtcac cagcccgatc gaaagaccct tgacggagaa cgcccgcccc gggagccagg
    29581 gcaaaagcag cggagttaaa acagctccgc cgaaaagagc tgcgaaaagc gcaacaactg
    29641 caaacacgcc gtcatggagc aggttggttt gaaatccgga tcgacccaga atacctgcca
    29701 gaaccaagag ggcgagcgat aggagcgcaa agggttttgc cgcgcccaga agttcaatcg
    29761 ggatgagcac ggcgcggtcc gccagcggaa aagtaacgat tctcatttcc ctggaggccc
    29821 taagcccatt gtccagatac gcagggatat ccctggcgcg aaccggaccg tagtgcgcct
    29881 tgaatccgga gagcttttta acctggtggg cggcgacgcc gggtgcgccc agttgcggga
    29941 gaaccatttt tctgtggcgg accacttttt tcaaaccgct ggcctcgatc cggtaaacca
    30001 gttcgttggt cccgaacgtg cctttccctg ccgcgcacca gacgttaatg cctttggtgt
    30061 ccaaaaccag aatccaggca ttccggccgg gcagcgcctg cctcagacag tcaaaactca
    30121 ttttgtaatt ggcggtgacc aggacgggcg cattttcatc cggttcgcct aacgcataaa
    30181 ggccgggatg gacagcgtag ttcattctgc cgactccaaa acgggctttg atcgttcccc
    30241 atcggtcctg acgcgtcaac cgggatgata ctttcggcag gaggcctgcg ggcgtggaaa
    30301 gagatccgga aacaaaaggc tggtccaaac tggaccccga atcgtctgca tccgctgcgt
    30361 tgtccccgcc gcagccgcat cccgcttgtt cctgaataat cggaaattca aatttgccgc
    30421 ccgcgtcaag gcctttcgta ttttcttcca tattttaccc tttatcttat tgacaacaat
    30481 cacaaatgcc ggccgcattc ctgacggcgt tcaagaaaat ctgcacagac tgcaggatat
    30541 catcgcttct gtcccccggc agatgatcgg ctattttatt gaaaaatcct tccagacata
    30601 actgaacatt ttgatgagta ttcctgcctt ccttcgtaag cctcagcaca aaggctctgg
    30661 aatcgcggct ggatttttgg cgggttaaaa ggcctttttg aatcagggga ttgaccagcc
    30721 gggtggtcgt acttttttcc acggaaagaa tccggtgcaa atctgagata ttcagctctt
    30781 catttttggc cacggcatcc agaataatgt actggtgaaa cgtaaccccc tcgcaaaaag
    30841 cggcatcctg cctgaaagat ctggttgcct gggcaagtcc gacaaaaacg ttcattaatc
    30901 cgtcttgctt tccatttttc ataatggttg tattatgcaa ctatattcgc gtttgtcaag
    30961 ggataatcat caataaactg ttggaaaagc gtccgtgcgg aaaatgccgg aacagggaaa
    31021 aatttaaaag cggtgagtgg aaagcgcatg aaaatggtcg ataaaacggg caataatcgt
    31081 ttgaatatta aaataaaaat gatattgttc cggcgcggcg tttccggaag acgatgccgc
    31141 ttgtccaaat aagatgatca gaaaggtccg gccatgaaaa accatgcggc aatagccgaa
    31201 gaaaagttca ccggcggtta caattgcgcc caatctgtct tgttttcctt ttgtgaagaa
    31261 ctgggcctcg atgccgacac ggccctcaaa attgcatgcg gattcggcgc cgggatggga
    31321 cgaaagggag aggtctgcgg cgctgtgtcc gggggcgtta tggtgctggg attgaaatac
    31381 ggcaggggag aaaaagagga gcgggcgcaa acggatgctt tgtatgccaa agtgcgcgag
    31441 ttgatcgacc gcttttctgc aaaacacggc tcctgtatct gccgggaact gctcagcggc
    31501 tgcaatctgg ccacgcccga ggggcaaacg tattaccggg aaaaccagtg cttccatggg
    31561 atctgccggc cctgtgttcg cgatgccgcc gaaatcgttg ccgacctgcc ggccatccgg
    31621 tagcgttcag gtaaaagaag gccgccgggg gggcgctgcg gggcgcagtc cctcctccgg
    31681 gagaaaacgc tgtgtttgga ttattccgtt gacacgcgcg acccctttcc ttatactcgc
    31741 ggccagcaaa aattgttcga ggaatcacca gatgacgatc aacgtcaaga ccgaagagca
    31801 tatccagcac atcgaagttg accggccgaa aaaattcaat gccatgactc aaggaatgta
    31861 tcacgagctg gcccaagcct atttcaggct tgatgccgat cccgaggccc gcgtcggcct
    31921 tgtgtatgcc gtcggccctc attttacttc cggtctggag ttgaccgact gggccggtac
    31981 gtttgacgcg ggcaagggct ttccttttgt cgcggaaaac gaaatcgatc ccttttacat
    32041 gatgagcgac aagcgctgcc gcaaaccgat tgttctggcc gttcatggct attgctacac
    32101 ctggggcttt gagatgatgc tcaatacgga catccgggtt gccgccactg acacgcgctt
    32161 cggtatgctg gaggtcaagc gcggattttt cccgggcgcc ggggcgacgc tcaggctggc
    32221 gaaagaaatc ggctgggcga acgcgatgcg ctacatgctg acgggcgatg aaatgagcgg
    32281 tccggaggcc taccgtctcg gactggttca ggagcttgct gaaccggggc agcagttcga
    32341 ccgcgcgctg gcgctcgcca gacatgtggc caaagccgct ccgctgggtg ttgcgcatgc
    32401 tttgagatta agccgtctgg ctgtgcttga gggagaagaa accgccaggg gattgattta
    32461 tccggctatg gaagaagtga tgaaaagtga agatatgatg gaaggtctgc aatcttttat
    32521 cgaacggcgc gccgcggtat tcaaggggaa ataatgcggt gtgcgctttt gccgggaaaa
    32581 gggcggacgc gacattaacg tttaacggca gtaaatgaag tgccttcatc agtgctcggg
    32641 ggttgtgtca gaatggtcaa aagctgtcat tcccgcgaag gcgggaatcc agagtctttt
    32701 aataatactg gattccgggt cgcgcttcgc ttgcccggaa tgacaaaata ggaattgcga
    32761 cgcagccttt cgtgcagggg gcatggattc gtttcatttt gcgcctttaa ttttcaacgg
    32821 gaggaattta agtatggctc atagtaattg gttgtatcag acccgcgaca tcatgttcca
    32881 gatcaaagaa tggctcggcg tggaaaagct cctggccctt gacgcttaca aggattacta
    32941 cgggatggac gacatcaaca gcatcctcga tgtcaactat aaagtctgcc gcgacgtgat
    33001 gtgtccggcc aacaaagacg ccgatgaacc cggcgcgaaa tttgtgggcg gcaacgagca
    33061 tgcggtgacg acgccggact cgttcaagaa tgtttataaa accattatgg atgcggagct
    33121 gggaccccag ttcggctacc gcggcgaagg caaaataccg ctgtgctggt atgcgccgat
    33181 tctggagatg cagtccgccg cgtcgccgtc gatcgtcatg ttctggtgtc tgacgcaggg
    33241 cgcgatcacc gtgcttcatg agtttgccac gcaaaaacag caggacctgt tcatccccaa
    33301 actgcgcacc ggcgagtggg gcggcactat ggggcttacc gaaccgggcg cgggctccga
    33361 agtcggcgca gtgcagagca aggcgtttcc cacagacacg cccggcaagt ataaactcac
    33421 cggaacgaaa tgcttcatct catcgggcga ccatgacctg gccgaaaaca tcatccatct
    33481 gatgctggcg aagacgccgg gcgccaaaga aggcaccgcg ggcatatcgc ttttcatcgt
    33541 gcccaagttc tgggtgaacg aggacggctc gctgggcgcc tggaatgacg tcaccaccat
    33601 gggcatcgag cacaagctgg gcattcacgg ctcgacgacc gccaccctct ccatgggtga
    33661 aaacgacaac tgctacggct ggatggtggg cgaagcggaa gtgaaagacg gacgcggcat
    33721 cggcatgaag cagatgtttg cgtatatgaa cgaggaacgc ctgaatacgg gcttgttctc
    33781 gctgggctgc atcggatctg cctattacgc cgcgctggat tatgccaagc tgcgcgtaca
    33841 gagcaaacat tccaccaatc ccaagggtcc cagcgtccgg atcatcgaac atgaagacgt
    33901 ccgccgcatg cttttgtccc agaaggccgt tctggaggct tgccgggcgc tcatttacaa
    33961 gtcctatctc taccgcgacc tggccgtgga tgccgccacg cccgaagaac gggaatacta
    34021 cgatgacatg tttgcgatca acaaccccat gtgcaaggcc tacacgtccg acatggcgag
    34081 gctcaccacc ggcgacgcca tccagtgcca cggcggctac ggcttcacgg aagagtatgc
    34141 cccggcggaa ctgtaccggg acgttgtgat ttacggcatc tgggaaggca ccaacttcat
    34201 ccaggcgcag gattataccg gccgcaagtt caccatgaag gacggcgcgc cgttcaaaaa
    34261 gtgggtggcg gaaatcgacg atttcgtcgc caaaaagaag acggacgcat tcgcggcgga
    34321 attcgcaatg atggcggaag ccatgacggc tttcaaagag atcgtccata tgaacgacgc
    34381 ctggacgaag ggcgaccgcc agatgaaaca gctttttgcc acccggaccc tgcatgcagg
    34441 cgcgagagtt tactgcggca tgctgctttt ggaccaggcg cttctggcgt cggccaggat
    34501 gaaagagaaa ggcgaaatcg acgccgattt ctacaagggc aaaatcgcca cggcccggtt
    34561 ctatatcatg aatcacgttc ccgaactctt cggttatctc aagtcgatga agttcggcga
    34621 ccgcagcgcg atcgacatcg ccgaagaaag ctt
//
LOCUS       DAOSRM010000009        34561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1382_length_34561_cov_95.415232, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000009 DAOSRM010000000
VERSION     DAOSRM010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 34561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34561
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1382_length_34561_cov_95.415232"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..653
                     /locus_tag="PLA58_01965"
     CDS             78..653
                     /locus_tag="PLA58_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61592.1"
                     /translation="MKRIFSILLFTILIPIYLNANDLKAVVSPEPKYYAWWLRITFMP
                     EHKELYGVPLNKIDPTWMLASQLKKEAIPREVLFESGEDEMAARGIDFMQKGDFNSDN
                     IEDLALVGVFQNLEKEQGMFFLILTKDKNDLWKKTFLQSWLGKPGFLGLQKDGGCLAL
                     LFCMDCGGVSEVTWDKKLKRYQIQKPADEET"
     gene            complement(1017..1092)
                     /locus_tag="PLA58_01970"
     tRNA            complement(1017..1092)
                     /locus_tag="PLA58_01970"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1057..1059),aa:Phe,seq:gaa)
     gene            complement(1152..1784)
                     /locus_tag="PLA58_01975"
     CDS             complement(1152..1784)
                     /locus_tag="PLA58_01975"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOR61593.1"
                     /translation="MTTYAFAGFWRRFIAYMIDGFIVSVVIIFLGVVAGVAYISGAMT
                     GDGRAIVAQMTDPAKLASLTAWIWVLTFAVNIAYFTYFHGSTGRTPGKRLLGLQVVSV
                     TGESISFGTAFLRSVGYLVSSLVFCLGYLWVAFDRRKQGWHDKIAGTVVIIREPQGSE
                     AGISIPDSSSGPQNPAGFGCIPVNASQPEEKKTEAPIASGAAGVDGQKMP"
     gene            complement(1781..2554)
                     /locus_tag="PLA58_01980"
     CDS             complement(1781..2554)
                     /locus_tag="PLA58_01980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOR61594.1"
                     /translation="MTLPRIYSPETLQNASQCVLGPDHLRYLKTVLRLKPGDAILVFD
                     GYGHEFEARIENFSASGVTVRLGASLPPVQRKIRVTLAQGIPKAGKMDGIVRTAAELG
                     TDVIIPVAAARSVRRMEGEKSSAKVARWQKIVQEAARCTRSSSVTVVEPVLSFADMLK
                     RADSGARKLIFWEEEDRLTIREVLSDGRFDGAAHYFIIVGPEGGLTREEVDRARESGF
                     ISVSLGRQILKVETAAAAILSIIQYEKGIFSDVAEGIGT"
     gene            complement(2740..3684)
                     /gene="prmA"
                     /locus_tag="PLA58_01985"
     CDS             complement(2740..3684)
                     /gene="prmA"
                     /locus_tag="PLA58_01985"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOR61595.1"
                     /translation="MTQKEPEKWLKIQISAPAEQIDALGNFLEETGAQGVFSESLMPP
                     GADDFPESTGPEIINAFFPADARTEKRMKAVQKYLKSLEEIFPDAAAPALSTETITDP
                     DWGEQWKKYFKPIRVCRNIVVKPTWERYAPDSRDIVVEIDPGMAFGTGQHASTRMCME
                     AIEDIILNDRSIGEWKVLDAGCGTGILGITAAKMGAQDVICVDNDPRATEIAAQNALI
                     NAVEDRIKICNKDAAQMSQPRNLIIANLTAKLLLKMRPHLVRQLLPGGYLIISGIIEA
                     DVPAMEEHFMVAPLVQYRRLTEKEWVCCVLRKENSGPS"
     gene            4044..6554
                     /locus_tag="PLA58_01990"
     CDS             4044..6554
                     /locus_tag="PLA58_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOR61596.1"
                     /translation="MKTALATEVHLKEPKALSGRIQWLRDYYFRGTERPWNNEFTCWT
                     TGTPWDVIYNEMTFYIVPETYTLLNTLGASYLQAARPVSLHPDFWKWSLVERRAWFVK
                     EAIVNCVPQEILPGDLLAGARFNIQTSMCFTKEESRAWQKLVKGKNGARAKMKWFHDH
                     GYGNAGATSGHLIPGHERLLQIGWKGVHAELMSRYNALSSAERAASKGAQLRAMMTAS
                     TMARDLAGRYRSLCEDLAQKETDAVRAAELRTMAANLARVPWEPPTNFWEAVQALWIN
                     HMLVMTDENYPGAGVSFGRADQYLYPYYEKSLRDGMQRDWTKEILKCFWVHANTAYDA
                     MIRNGNQGITAGFGQLITLSGLGADGRDMTNDLTYAILEVIDEMSPILEPKPNVRLHR
                     NSPEELYDKIVDMIESSQGAPFLLNFDERSMAGMMLEARKAGLAHLIHEGNVHEYAPV
                     GCLENTMVGNDRSGTVDNNLNLLKAVELALTGGKDLLPFYDPMTGKTEAILQDGPKTP
                     DTASMQTWEEFWEAYATQTRYIIQKCVDLYETSESIRARFFPTPYLSCMVEGCAEQGM
                     DVTQGGARISFTTLEAVTYATTVDSLLAVKYLVFDKKVCTLPELADALRANWKGHEVL
                     QAMAKNRAPKYGRDDDEADALALRVMQLWCDETWRHQTRSTGRRYRPGMLSWNYWAGD
                     GYIMAASADGRTKGQFLSNAICPSNGADTHGPTANANSVGKVLGGKAKDGRGDWLDYL
                     NVLPNGASHTITFNPSILRDPVHKDKFKAFLKGYTLNGGTALQINMLDPGMLKDAQDH
                     PENYRHLLVRITGYNAYFTTVGRELQNEVIERVSHRQF"
     gene            6567..7595
                     /locus_tag="PLA58_01995"
     CDS             6567..7595
                     /locus_tag="PLA58_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOR61597.1"
                     /translation="MTHAEAKQGRILQILRMSTEDGPGIRTSVFFKGCPLGCLWCHNP
                     ESISPKPQIQWLKTSCIGCGLCVETCPEKALEKTPDGIVINRLVCTGCGLCTEECPTT
                     AMELLGRKWSVPDLVRELVKDRVYFDESGGGVTLSGGEAALQHEFCLPLLKELRAKGI
                     STALDTCGQAPQKTLAALLPYVDVLLYDLKEIDPEKHKRFTGADNAKILENAVFAARF
                     KKTHPYPKTLWIRTPVIPGATDTPENIAGIGRFIREHLEGAVDRWELCAFNHLCRDKY
                     KRLGLNWAFAGAQPVEKSLMESLAKVAKGAAPETNVCWSGATRLAKSAESKEPAEKPN
                     KRQPACAG"
     gene            7620..8465
                     /locus_tag="PLA58_02000"
     CDS             7620..8465
                     /locus_tag="PLA58_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61598.1"
                     /translation="MTIKNRFSESDMKAFAPSEKVGIIATVTPAGLPHLSLLTSVMAS
                     TPTQMTIGEFCKGVSKDYLQQSRKAGFAILTLDKKLWRGKALWTHLKKEGPEYEVYNQ
                     QPMFRYNTYFGINTVHYLDLKETTEGAPLPLASIAASAILTKIAKGAAAQNAGAKALS
                     PFGKNLFDRLDSLKFLSFIDDDGFPAVIPVIQCQACDENRLAFYPGAFGEELDRLKAG
                     KTMAIFGLTMQMEDVLVRGVFQGYARRRGIRLGTLDIDWVYNSMPPNHGQIYPPLPLK
                     AVVHF"
     gene            complement(8481..8987)
                     /locus_tag="PLA58_02005"
     CDS             complement(8481..8987)
                     /locus_tag="PLA58_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOR61599.1"
                     /translation="MKKTRGILLIVWILLGVFVCAGSASAANETGRDGRFVAYDDGTV
                     LDTGANLMWADKDNGNDINWAAAKSYCENYSGGGYTDWRMPTKNELAGLYDGSRRDGR
                     GLHMATSLITLSGCCPWTSDTCISMMPQYDMAGYYAFTFSFSSGQSVCSHQGLDRDVR
                     VLPVRSSR"
     gene            complement(9007..9588)
                     /locus_tag="PLA58_02010"
     CDS             complement(9007..9588)
                     /locus_tag="PLA58_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR61600.1"
                     /translation="MNINDFRKQENGVHEIFLHRWSPRAMSGGDLDEATLLNLFEAAR
                     WAPSSNNNQPWRFIYARRNTPRWEVFFDLLAEGNQIWAKNAAVLIAVISKTTFDSGSP
                     ARTHSYDAGAAWVSLALQGSLKNLVVHCMQGFDYDKAKEVLRVPDDYAVEAMIAVGHP
                     GKKEDLPDYLQKREFPSPRKKLDEIVMEGVFKK"
     gene            9808..9883
                     /locus_tag="PLA58_02015"
     tRNA            9808..9883
                     /locus_tag="PLA58_02015"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9842..9844,aa:Glu,seq:ttc)
     gene            10250..10603
                     /locus_tag="PLA58_02020"
     CDS             10250..10603
                     /locus_tag="PLA58_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOR61601.1"
                     /translation="MEIKFKIWLEKDGHALFGEGRDQLLKAIDECHGLYGAAKQLKMS
                     YRAAWGKIKLTEERIGKKIVEVGDDKKMHLTKDARMLLAEFEKLEKDVDAFIQVRRSP
                     FIVLKDPLPKSDEKK"
     gene            10892..13450
                     /locus_tag="PLA58_02025"
     CDS             10892..13450
                     /locus_tag="PLA58_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOR61602.1"
                     /translation="MKRRSFLKLAGAAGVATAMTAFPFRNMRAAWAFGDHPQEQMPYR
                     ITKKVPQVCARACEADCAYNVVVGVDPATGLERALTLEGRPEDPVSNGKFCIKGMGFV
                     DSMYDPDRLMVSLKRTNKTKGTDVDPGWVVMKTEDAVNEIIAKMKTYKPEEILMASPG
                     DPYTNRLCQSIGCTRSDQRTECFGTHYYINCLTLTNPPNKFYSSCYTPSHHVCGYDYD
                     TCKYQVWFGFDSFSKCGKAGMLNHWAAGKRNGGKIVMFNPVRTPMTDNFASEYYAIKP
                     GTDLAVALAMINQMMAGKKYNKEFMSKYSDGAALVDVEKQTHLKTADGMFLAWSTKKK
                     KAEPIDECDDPALGGTFTVTIDGRQVKAKPVLQLLAEATKTYTPQWAAKISDVPAKAI
                     SRIARDFAAAAPYALIPTYKRDAAGPNYANSWRLRHAIQILNALAGSIDHEGGMLLLH
                     DVKIPWIDEIAPPVAPYPEQPAQPVDFRNEFPVTDNIYRKKDFSAPGHYGMVGWGLYK
                     TNRAKVAFFRNPHRGLFAMIHSQMFEAASQKLELVVDWNLYLDDLGYFCDYVVPAPHQ
                     FEEGKMDLRLYYPKYPCIVGGTPVQKSPGDQIGWGGFAMKIGLALAPKYWTTDGSGNP
                     AKKIPTNLSDVAVKKAGIAENAADFMKKGGLWIDKKEYQNYKQIEQIGYGKPRGRVKL
                     YINEFAEVGHSPLPVWHARWHETTGDYKFSLLITRAPWYMHADPNFINNPVLKEVTVR
                     NHMDTAWINPEAAAKLGLKEGDGVIIENDPTYMKDLPRPQKAKVHLTKRITRNDCVLL
                     FHGIGHRAKNLKVAANFGYRDGDLIPQKDPAMLKKFDPTGMGWVEDVFVSIKKM"
     gene            13463..14167
                     /locus_tag="PLA58_02030"
     CDS             13463..14167
                     /locus_tag="PLA58_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR61603.1"
                     /translation="MKKYAILLDDTFCTGCNSCAYRCIQEFRYHDQASKGLFRTFVQI
                     NDDGLYQKRCMHCLEPDCVENCPVKALTKSEYGPVLYNAQKCIGCQTCVRVCKFHVPQ
                     FDADTKKIVKCSMCAHRISEDKKPACAEVCPTGALQFGEYEAMKKLAAKLAKEKKLKL
                     YGYEENGGTHLFVLAKEDPTKFGYPKVAKKALKGKVSLDGGIGVPAMAALAVAGFKKY
                     SERRTRIEAEAKKAKK"
     gene            14272..14949
                     /locus_tag="PLA58_02035"
     CDS             14272..14949
                     /locus_tag="PLA58_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HOR61604.1"
                     /translation="MSNQKTTTRRTGPDDRLIGLQARAALFNLLAQAFNYPDQLLVEK
                     LVSGEFTATLNEWLNVLQPEAAWTDRIAVMERKYTAAGQNEAELLLEMEKDYTRMFFS
                     SKPRLVYLFESVYKEGKLLQESTFEIARLYYEAGLNLVDDFKLPPDHIAVELEFMAYL
                     YFNELAAMKKGNKENEALAQKLQKETLEKHLTNFGRTFAEKVVAHAATDFYKIMATVT
                     TSILAAL"
     gene            complement(15165..15338)
                     /locus_tag="PLA58_02040"
     CDS             complement(15165..15338)
                     /locus_tag="PLA58_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61605.1"
                     /translation="MSKLNLTPEEEAKVLEILERYLPELQNETVHTDNKELRKFFKER
                     QAFMEELMRRLKS"
     gene            complement(15433..15891)
                     /locus_tag="PLA58_02045"
     CDS             complement(15433..15891)
                     /locus_tag="PLA58_02045"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOR61606.1"
                     /translation="MPARKLKEYLDSQAVKYIKIAHSPAYTAREIAQSLHIKGGMVAK
                     TVIVVMEGKMAMAVLPANRSINFDHFRKAVGDQEIHLATEGQFKGLFPDCEVGAMPPF
                     GNLYDMEVYVDEHLREDEDIFFNACSHSELVRMSYQDFERLVNPRVIKLT"
     gene            16391..17704
                     /locus_tag="PLA58_02050"
     CDS             16391..17704
                     /locus_tag="PLA58_02050"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOR61607.1"
                     /translation="MDNLSNRKTAVAIFRAALASVDPCRLVQKHIPDVLDAYRRGNGQ
                     RLMLAAFGKAAFAMTRALAESPAGELITRGTVITKYGHVPGTLPDPIAVYEAGHPLPD
                     AAGVAATGRLMAMLENPDPQTLLVCLISGGGSALLVAPADGLTLAEKQATTQCLLAAG
                     ADIGELNAVRKHISGIKGGQLAALARPARVLSLILSDVIGDPLHVIASGPTAPDNTTY
                     ADALAVIGKYGLEERIPNSVYQRLIQGREGTVPETPAKDDPVFADVANLIIGSNKLAI
                     EAAAAKATAAGFETVVLTTELRGEAREAARWLAQQALAFQNQQPAGKKLCLVSGGETT
                     VTVTGTGKGGRNTELALAFAEEIDGRPGITLLSAGTDGTDGPTDAAGAVVDGQTIARA
                     NARGLSSDAFLSRNDSYTFFKKTDELLITGSTGTNVMDIQVVLLT"
     gene            17726..18382
                     /locus_tag="PLA58_02055"
     CDS             17726..18382
                     /locus_tag="PLA58_02055"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOR61608.1"
                     /translation="MTTIVILAIAVVLLGGLLFAEKKESLKGILLTKPFLSGLCIVTA
                     LLQPYAAGPYFYLILAGLVLCFAGDICLAFFFNRKVFAAGLAFFLTGHVAYILAFFMT
                     SGVNAGTAVSLAAVAVVSTYVFIRLRPHLGPMMGPVIAYIAVISIMVVGAASLAVRQD
                     LSFQARTLVMAGSIFFYASDLFVARHRFVKKSIFNRYAGLPMYYAAQFTLVFSVGMIP
                     "
     gene            18394..19698
                     /gene="larA"
                     /locus_tag="PLA58_02060"
     CDS             18394..19698
                     /gene="larA"
                     /locus_tag="PLA58_02060"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOR61609.1"
                     /translation="MKAEIRYHHTLLDIEFPDTSRIYRSCFEPPAAVESELVLQAIRN
                     PLCSESLVDSIKTRPRNSVMIAVSDITRPIPYRTFLPVLLDEIESTGIDRKQITLLIA
                     TGMHRPSTKEERAFMFGDDIAGRYKILDHDAEGDMVTLSGTSCSGAPAKLNRDFAKAD
                     YKIVIGLVEPHFMAGFSGGRKTLCPGLSSMETIKHFHGYEFLSHPKAGIALLDGNPCH
                     EEALSVAKLVDVDFSLQLVVNHAKRIVKAFGGDLFETHASACHYVKGKACPIVEAEAD
                     VVVTGCGGYPLDATFYQCTKALVTALPCVKKGGMIIAAGGCVEGVGSQTYENMLRKYS
                     NDFPRFIRNISRSTDIVKDQWQIQMQARVYEKTGPDNLYFFSHGIASGAGAFLGAKIM
                     EAGLERITGELQKAVDALARQGYSFAVIPEGPYCAPLSKGLV"
     gene            19892..20329
                     /locus_tag="PLA58_02065"
     CDS             19892..20329
                     /locus_tag="PLA58_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOR61610.1"
                     /translation="MKLSTRSRYGLRLMLALARNYGKGNTFLKEIAREEGISEKYLSL
                     IIIPLKGVGLVNSVRGASGGYTLAREPAQITLKDIMDVLEGDCLVDCLKDPLFCSRVN
                     ICASHDIWAMLSGRISETLSGITLEQLVTMNKDKLEKSVSPSI"
     gene            20355..21416
                     /gene="mnmA"
                     /locus_tag="PLA58_02070"
     CDS             20355..21416
                     /gene="mnmA"
                     /locus_tag="PLA58_02070"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOR61611.1"
                     /translation="MNKKILVAMSGGVDSSVAALLLKKEGYDVSGVTMCLGIATDDSA
                     KCCGASALDDAKRVCDRLQIPHYVFDYASQLEKKVIAHFISEYQKGRTPNPCVECNRH
                     LKFGTLLDKALSLAFDFLATGHYAAIGKNENGYFLARPKDKKKDQTYFLYSIPYQRLG
                     DILFPLARFTKEEVRDLAKEYSLPVAAKQESQDICFVTQKNSQEFLSERVRECKPGPI
                     VDLHGKLLGRHRGIIFHTIGQRAGLGISHPTPLYVLSIDSGRNLIVVGEKKDLMANGL
                     VASDVNILAENWPKEVHAKIRYRKKGALCRVTKEKDKLKVVFAEGQEAITPGQSVVFY
                     DHDRVLGGGIIDEVMHGIY"
     gene            21403..22317
                     /gene="nadA"
                     /locus_tag="PLA58_02075"
     CDS             21403..22317
                     /gene="nadA"
                     /locus_tag="PLA58_02075"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOR61612.1"
                     /translation="MEFIKEIERLKKERNAVILAHNYQIPEVQAIADYVGDSLGLSIQ
                     ASKTKADVIVFCGVHFMAETAKILSPEKKVLLPDPESGCPMADMITADELRKLKTEHP
                     QAAVLCYVNTSAEVKAECDMCCTSANAVGIVAEHMKGAEEIIFVPDKYLGTYVAGKTG
                     RDFILWQGYCPIHARILPEDVERQKEKHPHAEVLVHPECTPALTAIAGKVLSTEGMCR
                     RAAKSSNTEFIIATEVGILRRMAKENPEKKFYPASEQALCPNMKRTTLEKVLWSLQDL
                     KHEVDVPADIMIRARRSIEGMLSCQPQN"
     gene            22347..23642
                     /locus_tag="PLA58_02080"
     CDS             22347..23642
                     /locus_tag="PLA58_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOR61613.1"
                     /translation="MNIENSLKIETLALHGGQEPDPTTGSRAVPIYQTTSYQFKDTDH
                     AANLFALKEFGNIYTRLMNPTTDVLEKRLALLDGGVGALAVASGQAAITLALLNIAQA
                     GDEIVSADNLYGGTYNLFHHTFPRLGIKVNFVKSNDLKALQAAITPKTKAVYAESIGN
                     PKLDVADLYGIAAVAHKNGIPFVLDNTVSPYLLRPLDFGVDIVVYSATKFIGGHGTSL
                     GGVIIDSGKFDWTNGRFPLISGPDASYHGIDFVEALKPNGNIAYIIKARVTLLRDLGP
                     AISPFNSFLLLQGLETLHLRLPRHAENALATAQYLKTHPRVAWVNYPGLEDSPEKERA
                     QKYLSKGAGAILGFGIRGGAAAGKKFIESLRLISHLANVGDAKTLAIHPATTTHQQLS
                     PEEQFATGVTPDFIRLSVGLEHIDDILADIEQALQKAES"
     gene            23667..24596
                     /gene="cysK"
                     /locus_tag="PLA58_02085"
     CDS             23667..24596
                     /gene="cysK"
                     /locus_tag="PLA58_02085"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOR61614.1"
                     /translation="MTNLYDDITKTVGRTPLVRLNRITKDLPATFLVKLESFNPLSSV
                     KDRIGVAMIEDAEAGGLLNKDSVIIEPTSGNTGIALAFVCAAKGYRLILTMPETMSME
                     RRQLLNILGAELVLTEGAKGMRGAVEKAEELAKATPGSFMPQQFANPANPAVHRRTTA
                     EEIWKDTEGGIDFFISGIGTGGTITGVGEILKQKKPSVKIIGVEPADSPVLSGGKPGP
                     HKIQGIGAGFVPDVLNRKIIDEMIAVKHEDAGLTARRLAKEEGILAGISSGAALWAAL
                     QAGGKEQAKGKTIVVLLPDTGERYLSTWLFHER"
     gene            24723..25886
                     /locus_tag="PLA58_02090"
     CDS             24723..25886
                     /locus_tag="PLA58_02090"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOR61615.1"
                     /translation="MKKAKHEKTNIHSVGAVETKTFTFAEQLYLASGEKLGPVTLAYE
                     TYGSLNKEKSNAILILHALSGDAHAAGVHAGDNDTGWWEELIGPGKAFDTHRYFIICS
                     NVVGGCKGSTGPSSLNPATGKPYALDFPFITVADMVEAQRHLIDHLGIDKLLCVAGGS
                     MGGMQVLQWVSSYPERVKSAIPIATTLKHSPQQIAFNEVIRQSIMADPAWREGSYYEF
                     GQPEKGLSVARMIGHITFMSEHSMEEKFSRRLKSDRFSFGFDADFEVEGYLRYRGANF
                     VKRFDANSYLYITKAIDYFDLSGGKFLPHGKTVDTRFLIISFKSDWLYPPHQSQEIVR
                     ALKRKHVDATYCELTSTYGHDAFLIEVEEQTTLIKHFLDKTFNGYLVMGNDGI"
     gene            25873..26517
                     /gene="metW"
                     /locus_tag="PLA58_02095"
     CDS             25873..26517
                     /gene="metW"
                     /locus_tag="PLA58_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOR61616.1"
                     /translation="MMEFNTQLDHQIIYSIVKSDSRVLDLGCGEGELLYPLVRDKHIR
                     AQGIELNDQSIQECVKKGLSVFHDDIESGLREFPDNAFDYVIMNQSMQEVKKVDCVIQ
                     EALRIGTQVIVGFPNFAHRTSRFSLFFRGKSPVTKSLPYLWYDTPNVRFLSITDFRDF
                     CVEKNIRIVEEHYLGEKTVVRFFPNLFALNAIFVLTKKDEAALKSGGKGYRLVL"
     gene            26577..27401
                     /gene="nifU"
                     /locus_tag="PLA58_02100"
     CDS             26577..27401
                     /gene="nifU"
                     /locus_tag="PLA58_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HOR61617.1"
                     /translation="MWDYTDKVKEHFLNPRNVGEIENPDGVAEVGSLACGDALKLTFK
                     LDENKRIRDAKFKTFGCASAIASSSALTEMIKGMTVDEALKVTNQDIARYLGGLPDEK
                     MHCSVMGKQALEKAIENYRGAPSLEPGAKIICECFGVTDKEIERAIRENNLATVEDVT
                     NYTKAGGGCGNCQDAIAQILERVKAEAKTEVKPKLTNIQKIRLIEETIEREIRPSLKH
                     DGGDIEIIDIIGNRVIVALRGACATCKASNITLKDFVEQRLRELVVPDITVEEASK"
     gene            27398..28579
                     /gene="nifS"
                     /locus_tag="PLA58_02105"
     CDS             27398..28579
                     /gene="nifS"
                     /locus_tag="PLA58_02105"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOR61618.1"
                     /translation="MKTIYLDNNATTKVADEVLEAMLPYFNQFYGNPSSMHTFGGQIG
                     QRIRDAREQVAALLGALPEEIVFTSCGTESDNTAIYSALLTRPDKKHIVISRVEHPAV
                     RALCTHLSAQGYKITELPVDKNGMLDLEHLEKSLTPDTAIVSLMWANNETGVIFPVEE
                     AAKLTHDKGVLFHTDAVQAVGKIPINMKNNVIDMLSISGHKLHAPKGIGVLYIRRGTK
                     FSPFLIGGHQEKGRRGGTENTAGIIGLGRACQLAANHILTENVHVKQLRDKLERELLK
                     KIPQSRVNGDIQNRLPNTTNISFEYVEGEAILLLMNELGICASSGSACTSGSLQPSHV
                     LRAMGVPFTMAHGSIRFSLSVYNTEEEVDFVIEKMPPIIERLRGMSPFWSQAQQACTN
                     Q"
     gene            28589..29608
                     /locus_tag="PLA58_02110"
     CDS             28589..29608
                     /locus_tag="PLA58_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOR61619.1"
                     /translation="MMKRLNNGGKCREEIKSAKHFRKEVPDVVDGLVHSCNSAGCFDH
                     VSAEPIPHREAIINILHRLSLILYPGYFIRTRLDSTNLEYYLGEQVTALYETLSEQIV
                     LAIRHDCIRHNKSCVHCEPLGHQMTLAFLRKLPTLRFMLAKDIRASLEGDPAAKGYDD
                     IIFSYPGIRAITIYRVAHELYHLNIPLIPRIMTEYAHSRTGIDIHPGAHIGESFFIDH
                     GTGVVIGESCVIGNRVRIYQGVTLGAISLSKSEVKKLRSKKRHPSIEDDVIIYANATI
                     LGGATVVGARSIIGGNVWLTHSVPPDTEVFVNKQDLIFGAKQYKKQAAKQKLRQVNQN
                     HLIIK"
     gene            29623..30420
                     /gene="fdhD"
                     /locus_tag="PLA58_02115"
     CDS             29623..30420
                     /gene="fdhD"
                     /locus_tag="PLA58_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOR61620.1"
                     /translation="MNEKLFSTFDIKEIISDSGVIRLRDSEKDVIGEISLKIFINGTE
                     YASLLCLNQLTEELALGFLHSEGVIDSIGDVASISSNERLFAVMINLAEGKSVEKCES
                     LRSVTSGCGKCFTYINPLKHDKFATLSGENKFLLSNIIQSMKDFERRSEIYKTVGGVH
                     SVLFQHDEYSVFNEDIGRHNCFDKINGILVKEKKMHLVGEGLLFVSGRVSSEIITKVI
                     RMGVPVLVSRATPTSAAVNLAREYNVTLLGYVRKDSGFVYSGQERLI"
     gene            30430..30876
                     /locus_tag="PLA58_02120"
     CDS             30430..30876
                     /locus_tag="PLA58_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOR61621.1"
                     /translation="MKTIKGKILAVNRSHKKGEKKSNIDCGLFLENLGLENDAHAEPG
                     IRQVSLLARESIEKIRDKGLDVQYGDFAENLTTQGIDLPSLPVGAKLKVGEKVLLEVS
                     QIGKICHNRCNIFYTVGDCVMPREGIFAKVITGGEIKILDEITVID"
     gene            complement(31186..31704)
                     /locus_tag="PLA58_02125"
     CDS             complement(31186..31704)
                     /locus_tag="PLA58_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOR61622.1"
                     /translation="MAKLYRCTICGDAYIGASPPANCPFCGAHMEYIVEAKDAVVNFD
                     VSLSVKDQAKAEHALKVEISNSAFYMCAANQTDDPEGKILFKALGKIEAEHASIWRKI
                     LKLGSVPPGKESCYTPNVENLKESHARETRAIDFYRKAASESDHPRLRQIFAALVEIE
                     TDHLHLSEERLK"
     gene            complement(31741..32115)
                     /locus_tag="PLA58_02130"
     CDS             complement(31741..32115)
                     /locus_tag="PLA58_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOR61623.1"
                     /translation="MAKRNEVFKCEVCGHIVEVLHEGKGELVCCNQPMKLLTENTVDA
                     SKEKHVPAIEKMAGGFKVKVGSVAHPMEEKHYIEWIEVIADGKAYRQYLAPGQAPEAF
                     FPIEAATITAREYCNLHGLWKA"
     gene            complement(32140..32637)
                     /locus_tag="PLA58_02135"
     CDS             complement(32140..32637)
                     /locus_tag="PLA58_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOR61624.1"
                     /translation="MSKTEDALKEAFAGESQANRKYLAFAAKADQEGFAQAARLFRAA
                     AEAETIHAHAHLKALKGIRSTKENLQEAVAGETHEFKIMYPAMIEAAKVEGHKEAERS
                     FVFANEVEKIHAGLYQRMLDNLAGAKESYSYYICPVCGYTAEKEAPETCPVCGAKGKM
                     FKKVD"
     gene            complement(32750..33184)
                     /locus_tag="PLA58_02140"
     CDS             complement(32750..33184)
                     /locus_tag="PLA58_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOR61625.1"
                     /translation="MRTNTKEDLIGQLKDNGLKITPQRLAIIDALVENRYEHPGATLI
                     FHEARKKAGRVSLSTVYATLKEFSESGLIRQMQFDRLENRYDVDLSDHVNLICKRCGK
                     VMDYHVPAPLDPKNIVRKSGFVVTDTRIEFHGYCRDCLKRPD"
     gene            complement(33253..33783)
                     /locus_tag="PLA58_02145"
     CDS             complement(33253..33783)
                     /locus_tag="PLA58_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOR61626.1"
                     /translation="MSIDQILGLITGVVFGFLLQKGRVIRFEKQIGALLIKDMTIFKF
                     MLSSILVGMVGIFILSDMGIITFSHKPLNVGAVVIGAILFGIGWAVMGFCPGTSVAAV
                     GEGRVHALFAIAGMLVGAAVFAELYPFLQATVMAWQDFGKISLPGALGISRWVLVPIF
                     WAATIGLFVVFEKKGL"
     gene            complement(33780..34394)
                     /locus_tag="PLA58_02150"
     CDS             complement(33780..34394)
                     /locus_tag="PLA58_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOR61627.1"
                     /translation="MYGPFAASGGEWNPKRFNEGKEATMKLKTKDGGWNPYFAGALVG
                     ILAIASVCATTELMGKTNYLGASTTFVRVAGFVEKVFASEHVAQNAYFAATKIKVDWQ
                     FMLIIGIFLGSLISSLTDKSFQVEKVPPIWRERFGSSMTKRGAGAFLGGIVAMIGARL
                     ADGCPSGHGLSGMMQLSASSFVALALFFAAGALTAALVYRRRAS"
BASE COUNT         8456 a         9273 c         9218 g         7614 t
ORIGIN      
        1 cggatagtgt aagatgataa gcaaataatc gaaaaaagcc cgacgcgtat attaattgtt
       61 atttttaggg catatatatg aaacgaattt ttagcatact gctattcaca attttaatac
      121 ccatatactt aaacgcgaat gatttaaagg cggtcgtatc gcccgagcca aagtattatg
      181 cttggtggtt gcgtataaca tttatgccag aacataaaga gctttatggc gttccgctca
      241 ataagattga tcctacatgg atgcttgcgt ctcaactaaa aaaggaagca attccccgtg
      301 aagtcctatt cgagagcgga gaagatgaaa tggctgctcg cgggattgat tttatgcaaa
      361 aaggtgattt caatagtgat aatattgaag atcttgctct tgttggagta tttcagaatt
      421 tagaaaagga acaaggaatg ttttttctta ttcttacaaa agacaagaat gatttatgga
      481 aaaagacgtt tttgcaatcg tggctaggta agccaggatt tctcggttta caaaaagatg
      541 gggggtgtct tgccttattg ttttgcatgg attgtggcgg tgttagtgaa gtaacgtggg
      601 acaaaaaatt aaagagatat cagatccaga aaccagcgga tgaagagacg taaagaaaca
      661 tttgtaattg cgttacaaac gccagttgga gcggttacaa aggttaacat aatttgaaac
      721 caccataaag caatgcctta tcaattcaat gatcgaacat gctgagcatc ctgaaataaa
      781 acgaaaataa gtatcccgaa tgaagcggct agaaactggg caagaacacc cgtccgggat
      841 ccggggcctg ctcacggcgt gcggacggca cgaacgctgt gacccctttg gtagccgggc
      901 caggcctcgt aggtccgccc gctgctgaaa tccagggcta cccgccagtc ccctttaagt
      961 gccctaaaaa aagaggtcac aaaccgtatt tacgacttgt aacctcttga tttcactggt
     1021 gggtcagggc agaattgaac tgccgacact cggattttca gtccgatgct ctaccgactg
     1081 agctaccgac ccgaacatat ggccaaacct gtttaaaggc cgaaaacctc tataaaactt
     1141 gactcccttt gtcaaggcat tttttgcccg tccacccccg ccgcgccgct ggcaataggc
     1201 gcttccgtct ttttttcttc cggttgtgaa gcgttgacag ggatgcagcc gaatccggcg
     1261 ggattttgcg gtcctgatga ggaatcggga atcgaaattc cggcttcact tccctgcggt
     1321 tcgcgaatga tcactaccgt tccggcaatc ttgtcgtgcc agccctgctt cctccggtca
     1381 aacgcaaccc agagataccc gaggcagaaa acgaggctgg agaccaggta gccgaccgat
     1441 ctcaaaaatg ccgtgccgaa ggaaatcgac tccccggtga cggagaccac ctgcaggccc
     1501 agcagccttt ttccgggtgt gcgtccggtc gaaccgtgaa aataggtaaa gtaggcgata
     1561 ttgacggcaa acgtcagaac ccagatccag gcggtcagcg aagccagctt ggccgggtcc
     1621 gtcatctggg cgacgatcgc cctgccgtcg ccggtcattg cgccggaaat gtaggccacg
     1681 cctgccacaa cccccagaaa gatgatcacg acgctgacga tgaatccgtc gatcatgtac
     1741 gcgatgaaac ggcgccagaa gcccgcgaat gcgtatgttg tcatgtcccg atcccttccg
     1801 cgacgtcgct gaatatgcct ttctcgtact ggatgatcga cagaatcgcc gccgccgctg
     1861 tttccacttt taaaatctgc cggccgaggc tcacggaaat aaaaccgctt tccctggccc
     1921 gatccacttc ctcccgggta agtccgccct ccggcccgac aataataaaa taatgcgccg
     1981 cgccgtcaaa ccggccatcg gacaaaacct cccgaatcgt tagccggtcc tcttcctccc
     2041 agaaaatcag cttgcgcgcg ccggaatccg cgcgtttcag catatcggca aaggacaata
     2101 ccggctccac cacggtcacg ctggaactcc gcgtacagcg cgccgcctcc tggacgatct
     2161 tttgccagcg ggcaactttt gccgacgatt tttccccttc catccttcgg acggaacgcg
     2221 ccgccgccac gggaatgatg acatccgttc ccagctccgc ggccgtcctg acgatcccgt
     2281 ccattttccc cgccttcgga atgccctggg ccagcgtcac gcggattttc ctttgaaccg
     2341 gcgggaggga cgcgccaaga cggaccgtca ctcccgacgc gctgaaattt tcgatgcggg
     2401 cttcaaactc atggccataa ccgtcaaaga ccaaaatggc gtcgcccggt ttcaggcgca
     2461 gaaccgtctt cagataccgg agatgatccg ggccgagcac gcattgcgaa gcattttgca
     2521 gcgtctcggg actgtagatt cggggcaatg tcaacgtcag gcactttccg agagctttgc
     2581 agaatcctac aactccagca cctttgtcat accggagcag gccggtatcc agtatgatca
     2641 agtatttcct ggattccagc cttcgcggga atccaggaaa atggagttgt gcaaaggtcc
     2701 cttttccgtc aaaatgattg gcctgccgcc cggaagcgat caggatggtc cgctgttttc
     2761 tttgcgcagc acacagcaga cccattcttt ctcggtcagc cgacgatact gaaccagcgg
     2821 cgcaaccatg aaatgctctt ccatggcggg cacgtcggcc tcaattattc ctgaaataat
     2881 cagataaccg ccgggcagaa gctgacgcac cagatgcgga cgcatcttca gcagcaattt
     2941 ggccgtcaaa ttggcaatga tcagattgcg cggctgactc atttgcgccg cgtctttgtt
     3001 gcagattttg attcggtctt ccaccgcgtt gatgagcgcg ttctgcgcgg caatttccgt
     3061 ggcccgaggg tcgttgtcca cgcaaatcac atcctgcgcg cccattttgg cggcggtaat
     3121 gcccagaatg cctgtgccgc atccggcgtc cagcaccttc cattcgccga tcgaacggtc
     3181 attcagaata atatcctcaa tcgcttccat gcacatgcgg gtggaagcgt gctgaccggt
     3241 tccgaaagcc atgcccggat cgatttccac gacaatgtcg cggctgtccg gcgcgtatcg
     3301 ttcccacgtc ggcttgacga caatattcct gcacacacgg atcggcttga aatacttttt
     3361 ccactgctct ccccagtcgg gatcggtgat ggtttcggta gaaagcgcgg gggctgctgc
     3421 atccggaaag atttcttcaa ggcttttgag atatttttga accgccttca tccgtttttc
     3481 ggtgcgcgcg tccgcgggga aaaatgcgtt gatgatttcc ggtccggtag actcgggaaa
     3541 atcatcggcg ccgggcggca tcagcgattc ggaaaacacg ccctgcgcgc ctgtttcttc
     3601 cagaaaattg ccgagggcgt cgatttgttc cgcaggcgcc gaaatctgaa ttttcaacca
     3661 tttttcgggt tctttttgcg tcatggtcca gccttcaatg ctgtagtgaa aaaaaccggt
     3721 ttttatatag cctgttcttt caaatatatc aagtcgcgcg gggcagagcc gggcggtgcg
     3781 taaatccaaa ccggaggggc attccttctc tgtgtgagga aatccgccgg acaggttttg
     3841 gcaccggccc ggacttggcc gtccgtcagg atttggcttg acaaaaacaa gcccggggac
     3901 ttattttgac accggtaatg ctacctgccg ggaaacaaag accgtttccg tgatgaaggc
     3961 cgtcttaaaa ccacgggaaa cggcatcttc ggtgatccgg cagaaaagcc tgattgcgca
     4021 aaccttttta ccgggaggca accatgaaaa ccgcactggc aaccgaagtt catctgaaag
     4081 agcccaaagc attatccggc cgtatccaat ggcttcgcga ttattatttc cggggaaccg
     4141 agcggccctg gaacaacgaa ttcacctgtt ggacaaccgg aaccccctgg gacgtgatct
     4201 acaatgaaat gacgttttac attgtgccgg aaacctacac gctgctcaac acgctgggcg
     4261 cctcctatct tcaggccgcg cggcccgtgt cgctgcatcc ggatttctgg aaatggtccc
     4321 ttgtggagcg gcgggcctgg tttgtgaagg aagccatcgt aaactgcgtg ccgcaggaaa
     4381 tcctgccggg ggatttgctc gccggcgcga ggttcaatat tcaaacgtcc atgtgcttca
     4441 caaaagagga aagcagggcc tggcaaaagc tggtcaaggg gaaaaacggc gcaagggcga
     4501 aaatgaaatg gttccatgac cacggatacg gaaacgccgg ggccaccagc ggccatctga
     4561 tccccggcca tgagcggctg ctgcaaattg gctggaaagg cgtccacgcc gaactgatgt
     4621 cccggtataa cgccctcagc agcgctgagc gagccgcatc caagggcgca cagctccgcg
     4681 ccatgatgac cgcgtccacc atggcgcgcg atctggccgg gagataccgg tccctgtgtg
     4741 aggatctggc gcaaaaggag acggacgccg tccgggcggc cgagcttcgg accatggccg
     4801 cgaatctggc ccgcgtgccg tgggagcccc cgacgaattt ctgggaagcc gtgcaggcgc
     4861 tctggatcaa tcacatgctg gtcatgacgg acgagaacta cccgggcgcg ggcgtttcct
     4921 tcggccgggc ggaccagtat ctttacccgt actacgaaaa atctctccgc gacggcatgc
     4981 agagggattg gaccaaggaa atcctgaaat gcttctgggt ccacgccaac acggcatacg
     5041 acgccatgat ccgcaacggc aatcagggca ttaccgccgg cttcggccag ttgatcacgc
     5101 tctccggcct gggggccgac ggacgcgaca tgaccaatga tctgacttac gccatcctgg
     5161 aagtgatcga tgaaatgtcg cccatcctgg agcccaaacc gaatgtacgg ctgcatcgca
     5221 acagtcccga agagctctac gacaaaatcg tggacatgat cgaatccagc cagggcgcgc
     5281 ctttcctgtt gaatttcgac gagcgctcca tggccggcat gatgctggag gcccggaagg
     5341 cggggcttgc gcatctgatc cacgagggca acgtccacga atacgcgccc gtcggatgtc
     5401 tggaaaacac catggtcggc aatgaccgct ccggaaccgt ggacaacaac ctcaatctgc
     5461 tcaaggccgt cgagctggcg ctcacgggcg gaaaagatct cctgcccttt tatgatccca
     5521 tgaccggaaa aacggaagcc atcctgcagg atggccctaa aacgccggac accgcatcga
     5581 tgcaaacctg ggaagaattc tgggaagctt atgcaaccca gacgcgatac atcattcaaa
     5641 aatgtgttga cctctacgaa acgtccgaat ccatccgcgc ccgttttttc cccacgcctt
     5701 atctttcctg catggtcgag ggctgcgccg agcaggggat ggacgtcacg cagggcggcg
     5761 cccgcatcag tttcaccacg ctggaagccg tgacctacgc gaccacggtc gattcactcc
     5821 tcgccgtcaa gtatctggtc tttgataaaa aagtctgcac gctgcccgag ctggcggacg
     5881 cgcttcgggc caactggaag gggcacgaag tgctccaggc catggccaaa aaccgcgccc
     5941 ccaagtacgg ccgcgacgac gatgaggcgg acgcgctcgc cctgcgcgtc atgcagctct
     6001 ggtgcgacga aacgtggcgg caccagacca ggtccaccgg gcgcaggtat cgcccgggta
     6061 tgctcagctg gaactactgg gcgggcgacg gctacattat ggcggccagc gccgacggac
     6121 gaacaaaggg ccagttcctt tccaacgcga tctgtccgtc caacggggcg gatacgcacg
     6181 gtccgaccgc caacgccaat tccgtcggga aagtgttggg cggcaaagcc aaagacggcc
     6241 ggggcgactg gctggactat ctgaacgtgc tgccgaacgg ggcgagccat acgatcacgt
     6301 ttaatccgtc gattcttcgc gatccggtgc acaaggataa attcaaggcg tttctgaaag
     6361 gctataccct gaacggtgga accgcgttgc agatcaacat gctggatccc ggcatgctga
     6421 aagacgcgca ggaccatccc gaaaactacc ggcatcttct ggtgcggatc accggataca
     6481 acgcctattt caccaccgtg ggaagggaac tgcaaaatga agtcattgaa cgggtcagcc
     6541 accggcaatt ttaaggcgga caagcgatga cgcacgccga agcgaaacag ggccggattc
     6601 tgcaaattct ccggatgtcc acggaagacg ggcccggcat ccgcacgagc gtttttttca
     6661 aggggtgccc gcttggctgc ctgtggtgcc acaatcccga aagcatcagc ccaaagcctc
     6721 agattcagtg gctcaaaaca agctgcatcg gctgcggcct ctgcgtggaa acctgcccgg
     6781 aaaaagcgct ggagaagacg cccgacggca tcgtcatcaa ccggcttgtc tgcaccggct
     6841 gcggcctgtg cacggaagaa tgccccacga ccgccatgga gcttctgggc aggaaatgga
     6901 gcgtgccgga tctcgtgcgc gaactggtca aggaccgcgt ttacttcgac gagtccggcg
     6961 gcggcgtgac gctgtccggc ggagaggcgg cgctgcagca tgaattctgc ctgccccttc
     7021 tgaaagagct gagagccaaa ggcatttcaa cggccctgga cacctgcggg caggcgcctc
     7081 aaaaaacgct ggccgccctt ttgccgtatg tcgatgtcct cctgtatgat ctcaaggaaa
     7141 tcgatccgga aaaacacaaa agattcacgg gagcggacaa cgcgaaaatt ctggaaaacg
     7201 ccgtcttcgc agcgcgcttc aaaaaaacgc acccgtatcc gaaaacactg tggatcagaa
     7261 cccccgtcat cccgggcgca accgacacgc ccgaaaatat cgcgggcatc ggccggttca
     7321 tccgcgaaca tctggaggga gcggttgacc gctgggagct gtgcgctttc aaccatttat
     7381 gccgggacaa gtacaaacgg ctggggctca actgggcatt tgccggcgca caacccgtgg
     7441 aaaagtcgct catggagagc ctggcaaaag tcgcgaaagg cgcggcgccg gagacgaacg
     7501 tctgctggag cggagcgaca agactggcga aaagcgcgga gtccaaagag ccggcggaga
     7561 aaccgaacaa gcgccaaccg gcctgcgccg gctgatctct aaaggaaagg aaaaagcgca
     7621 tgaccatcaa aaaccgtttt tccgaatcgg acatgaaggc cttcgcaccg tcggagaaag
     7681 tcggcatcat cgcgaccgtg acgccggccg gcctgccgca cctgtcgctt ctgacctcgg
     7741 tcatggcctc caccccgacg cagatgacca tcggcgagtt ctgcaaaggg gtgagcaaag
     7801 actatctgca gcagagccga aaagcaggct ttgccattct gacgctggac aaaaaactct
     7861 ggcgggggaa agccctctgg acccatttga aaaaggaagg gccggaatac gaggtataca
     7921 atcaacagcc gatgttccgc tacaatacgt atttcggaat caacacggtc cactatctgg
     7981 atctgaagga aacgacggaa ggagcgccgc tgcctctggc ctccatcgca gcgtcggcga
     8041 ttctgaccaa aatcgcgaaa ggcgccgccg cgcaaaacgc cggagcgaag gcgctttcgc
     8101 cattcggaaa aaatctgttc gaccgcctgg actccctcaa atttttgtca ttcattgacg
     8161 acgacggatt tccggccgtc atccccgtca ttcaatgcca ggcctgcgat gaaaaccgtc
     8221 tggcatttta tcccggcgcg tttggggaag agctggatcg actgaaagcg ggaaagacga
     8281 tggcgatctt cggactgacg atgcaaatgg aagatgttct ggtccggggc gttttccagg
     8341 gctatgcccg ccgccggggc atccggctgg gaacgctgga tatcgactgg gtttataatt
     8401 ccatgccccc caatcacggg cagatctacc cgccgcttcc cctgaaagcg gtggtccatt
     8461 tctgatttcg ctttgacgcg ctaccggctg gaacgcaccg gaagaaccct gacgtcgcgg
     8521 tccagcccct gatgcgaaca gacgctctgg ccgctgctga aggaaaaagt gaaagcgtag
     8581 tagccggcca tatcatactg cggcatcatg gagatgcagg tatccgacgt ccacgggcaa
     8641 cagccgctta gggtgatgag ggacgtggcc atgtgaagac cccggccgtc gcgccgcgag
     8701 ccgtcgtaca atcccgccag ttcattcttc gtcggcatcc gccagtctgt gtatccgccc
     8761 ccgctgtaat tttcacaata ggatttggcg gcggcccagt tgatgtcgtt gccgttgtct
     8821 ttatcggccc acatcaggtt ggcgccggta tccagaacgg tcccgtcgtc atacgcgaca
     8881 aaccgtccgt ccctgccggt ttcattggcc gcggacgcgg atccggcaca gacgaaaacc
     8941 cccagcaaaa tccagacaat cagtaaaatt ccccttgttt ttttcatgat gatcctctct
     9001 gtgcggctac tttttaaaaa ccccttccat cacgatttca tccaattttt ttcgcggcga
     9061 aggaaattcc cgtttctgaa gataatcggg aaggtcctct ttcttcccgg ggtggcccac
     9121 ggcaatcatc gcctctacgg cgtaatcatc gggcacccgc agcacttctt tggctttgtc
     9181 gtagtcaaat ccctgcatac agtgcaccac cagatttttc agcgatccct gcagcgcgag
     9241 actcacccat gcggcgcccg cgtcatacga atgtgtccgc gcggggctgc ccgaatcaaa
     9301 cgtggttttg gaaatcacgg cgatcagaac ggcggcgttt ttcgcccaga tttggttccc
     9361 ctccgccagc agatcaaaga aaacctccca gcgaggcgtg ttgcggcggg cgtaaataaa
     9421 ccgccagggc tgattattgt tggaggaagg cgcccagcgc gccgcctcaa aaaggttcaa
     9481 aagtgttgcc tcatcgagat ccccgccgga catggcccgg ggagaccagc gatgaagaaa
     9541 tatttcgtgg acgccatttt cctgcttgcg aaagtcattg atattcatcg tttacctcct
     9601 gcagcctgat gatccggatg gttgccctct gattcagtct ccccgagact tttcttcgcc
     9661 agatggccgt cacagagcca tgtttttaaa aagattaacc ggttttattc agaatgcaag
     9721 cctttttaaa aatagtggag gcggagcttg actttccgga ttgaaaaata tataaggcgc
     9781 aacttgcttg cacgccaggc cgataacgtc cccatcgtct agaggcctag gacacgggcc
     9841 tttcacgcct gtaactgggg ttcgactccc catggggacg ccaataataa aaacgagggg
     9901 ttatgttacc cctctttttt ttgtcatcct aactcccatc gaaatattcg agcaaagcat
     9961 aagaaaacgc agccccagcg aacttaagcc ccggaccatt cccgaacagt aatattcaac
    10021 gatctgacaa agaattttcg gaaatatatt cccgaccggt catatacacc aaaaagaagt
    10081 tgctttgccc ggaaaaaaat ataagaatat tacatattca aatgtttaag acggcggctt
    10141 ggcaaatcct tttgttattt ttgcgttgct gcacccgcga cggatgctcc ctccggcaat
    10201 caagccggtt atgggaaagt ccggaaaaga atattttttg aaggtgatta tggaaattaa
    10261 atttaaaata tggctggaaa aggacggcca cgccctgttc ggtgagggac gcgaccagct
    10321 cttgaaagcc atcgatgagt gtcatggcct gtacggggcg gccaaacaat tgaaaatgtc
    10381 ctatcgcgcg gcctggggaa aaattaaatt gacggaagag cgcatcggca aaaaaattgt
    10441 tgaagtgggc gatgacaaga aaatgcacct gacaaaagac gcgcgaatgc tgctggctga
    10501 attcgagaaa cttgaaaaag acgtcgatgc atttatccaa gtgcgccggt ccccgtttat
    10561 cgtgcttaaa gatccccttc cgaaaagcga tgaaaagaaa tgagtattga acgcataccc
    10621 cccttctttc atctggttat gttccaccgg gcatatgctc tataacacat aactatatga
    10681 aataactgaa aaattacctt gacaatccct tttaatcctt tatgctgaac ttagcagaat
    10741 ggtcgacgcc ttaacggcgc aggctgtctg gacaagaaaa tttcgagtgt cggacgatgt
    10801 tcggtgaaac cgaacactcg aatgacttgg aaagaatatt ttgtcggttc catgtgtaaa
    10861 ggaggtaatg tcaatggaaa agaagatctc aatgaaaaga agaagttttt tgaagctggc
    10921 gggagccgcc ggtgtcgcca cggcgatgac ggcttttccc ttcagaaata tgcgggccgc
    10981 ctgggctttc ggggatcacc cccaggagca gatgccgtac cgcattacca agaaggtgcc
    11041 gcaggtttgc gcacgggcct gcgaagcgga ctgcgcctat aatgtcgtgg tcggcgttga
    11101 ccccgccacc ggtcttgaac gagcgctgac cctggaaggc cgtccggaag atcccgtttc
    11161 caacggaaag ttttgcatca agggcatggg gtttgttgat tccatgtacg atcctgaccg
    11221 cttgatggtt tctctgaaaa ggaccaacaa gacgaaggga actgacgtgg atcccggctg
    11281 ggtcgtcatg aagacggaag atgcggtcaa cgaaatcatt gccaagatga aaacctacaa
    11341 accggaagaa attctgatgg cgtcgccggg cgatccctac accaaccgcc tgtgtcagtc
    11401 catcggctgc acccgcagcg atcagcggac ggaatgtttc ggcacacact actacatcaa
    11461 ctgtctgacg ctgaccaatc cgccgaacaa gttttattcc agctgctaca ccccgagcca
    11521 ccatgtgtgc ggttatgatt acgacacgtg caaataccag gtctggttcg gattcgactc
    11581 tttttccaag tgcggcaaag cgggcatgct caatcactgg gcggcgggaa aaagaaacgg
    11641 cggcaagatc gttatgttca acccggtccg cacgcccatg acggacaatt tcgccagtga
    11701 gtattacgcg atcaagccgg gaacggatct ggccgttgcg ctggccatga ttaatcaaat
    11761 gatggccggc aaaaagtaca acaaggaatt catgtccaaa tattcggacg gagcggctct
    11821 ggtggacgtt gaaaagcaga cgcatctgaa aacagccgac gggatgtttc tggcctggtc
    11881 cacaaaaaag aaaaaagccg aacccatcga tgaatgcgat gacccggctc tcggcggcac
    11941 ctttaccgtg acgatcgacg gacggcaagt caaggccaag ccggttctgc agcttctggc
    12001 cgaggcaacg aaaacctata cgccccagtg ggcagcgaag atcagcgatg ttccggccaa
    12061 ggccatcagc aggattgcca gggattttgc ggccgccgcg ccttacgcgt tgattcccac
    12121 atacaaacgt gacgcagcgg gtcccaatta cgccaacagc tggcggctcc gccacgccat
    12181 ccagattcta aacgccctgg ccggctcgat cgaccatgaa ggcggcatgc tcctgctgca
    12241 tgacgtcaag attccctgga tcgatgagat tgccccgccc gtggcgcctt atcccgagca
    12301 gcccgcccag ccggtggact tccgcaatga atttccggtc accgacaata tttaccggaa
    12361 aaaagatttt tcggcgccgg gtcattacgg catggtggga tggggacttt acaagacgaa
    12421 ccgcgcaaag gttgccttct tccggaaccc gcaccgcgga ttgtttgcca tgatccactc
    12481 ccagatgttc gaggccgcct cgcagaaact ggaactcgtg gtggattgga acctgtacct
    12541 ggacgatctg ggctacttct gcgactacgt cgtgccggcg ccccaccagt ttgaagaggg
    12601 caagatggac ctgcgtctct actatccgaa atatccctgc atcgtcggcg gtacgccggt
    12661 gcagaaatcg ccgggcgacc agatcggctg gggcggcttc gccatgaaga tcggtctggc
    12721 gctggcgccg aaatactgga caacggacgg cagcggcaat ccggcgaaga agattccgac
    12781 aaatctgagc gatgtcgcgg tcaagaaggc ggggattgcc gagaacgccg cagatttcat
    12841 gaagaagggc ggtttgtgga tcgataaaaa agagtatcag aactacaagc agatcgagca
    12901 gatcggctac ggcaagccca gaggaagggt gaagctgtac atcaacgaat ttgccgaagt
    12961 cggccacagc ccgctgcccg tctggcatgc ccgctggcat gaaaccacgg gagactacaa
    13021 gttctcgctg ctcatcaccc gggcgccttg gtatatgcat gccgatccca atttcatcaa
    13081 caaccccgtc ctcaaggaag tgacggtccg caaccacatg gatacggcat ggatcaatcc
    13141 cgaggctgcg gccaagctgg gtctcaagga aggcgacggc gtcatcatcg agaacgatcc
    13201 gacctacatg aaggatcttc ctcggccaca gaaagccaag gtgcatctga cgaagcgcat
    13261 cacaagaaac gactgcgtcc tgttgttcca cggcatcggg caccgggcca agaacctgaa
    13321 ggtggcggcc aatttcggtt accgggacgg cgatttgatc ccgcagaagg atccggcgat
    13381 gttaaagaag ttcgatccga ccggaatggg atgggttgaa gatgtttttg tcagcataaa
    13441 gaagatgtag tgaggtgacg aaatgaaaaa gtatgccatt ttactggacg acacattctg
    13501 cacgggatgc aactcctgcg cctaccggtg catccaggag ttcagatatc acgaccaggc
    13561 gtcaaagggg ctcttccgaa cctttgtcca aatcaatgac gacggacttt atcaaaagcg
    13621 ttgcatgcac tgtctggagc cggactgcgt tgaaaactgt ccggtgaaag ccctgaccaa
    13681 atcggaatac ggtccagttc tttacaatgc ccagaagtgc atcggctgcc agacctgcgt
    13741 ccgtgtgtgc aagttccatg ttccccaatt tgacgcggat acgaagaaga tcgtcaagtg
    13801 cagcatgtgc gcgcacagaa tcagcgagga caagaaaccg gcctgcgcgg aggtttgccc
    13861 caccggcgcg ctgcagttcg gcgaatatga agcaatgaag aaactggcgg caaagctggc
    13921 caaagagaag aaactcaaac tgtacggcta cgaagaaaac ggcggaaccc atctgttcgt
    13981 tctggccaag gaagatccca cgaagttcgg ctatcccaag gtggcaaaga aggccctcaa
    14041 gggcaaggtt tctctggacg gcggcatcgg cgttccggca atggcagctc tggctgtcgc
    14101 aggcttcaaa aagtacagcg aaagaaggac ccgtattgaa gcggaggcca agaaggcaaa
    14161 gaagtaacga aaccatgaac cgcgttccgg cggcgccttc cggcgccgcc ggaacgtttg
    14221 ccgatttgtc ggtcattgga caatcgggcg gacatttgtt aggaaatatt tatgagcaac
    14281 caaaaaacaa caacgcgccg gaccggaccg gacgatcgtt taatcggtct gcaggcccgg
    14341 gcggcgcttt tcaacctcct ggcgcaggca tttaactatc cggatcagct ccttgtggaa
    14401 aaactggtct cgggggaatt cacggccaca ttgaacgaat ggctcaatgt cctccagccg
    14461 gaggccgcct ggaccgatcg gatcgcggtc atggaaagaa aatacaccgc cgccggtcag
    14521 aacgaggcag agctgcttct ggagatggaa aaagattata cccggatgtt cttttcgtca
    14581 aaaccccggc tggtttatct tttcgaatcc gtttacaaag aaggaaagct tctccaggag
    14641 tcgacctttg aaatcgcgag gctgtattac gaagcggggc tcaacctggt ggacgatttt
    14701 aaactaccgc ccgatcacat cgcggtcgag cttgaattca tggcctatct gtacttcaac
    14761 gagcttgcgg ccatgaagaa aggcaacaag gaaaacgagg cgctcgccca gaaactgcaa
    14821 aaagagactc tggaaaagca tctcacgaat tttggacgga cgtttgcgga aaaggtcgtc
    14881 gctcatgccg caacggattt ttataagatc atggcgaccg tcactacctc catcctcgca
    14941 gccctataaa aactgcttat cgcgggaggt gtttatgtgc gatacctgaa gcattccgga
    15001 aggcggaacg tcaggaaaaa gccatgacga tccggatgaa agaaattcaa aaggcggcct
    15061 ccgccccggc catccgcttt tggaaacgga tggccggccg gtcgggcgtt cctctggcat
    15121 agggggaaaa gcctggattc aggtcgttga aatattcgtt atgctcagct tttcaaccgc
    15181 ctcatcagct cttccataaa cgcctggcgt tccttaaaaa atttgcgaag ttctttattg
    15241 tccgtatgca ccgtttcatt ctgcagttcc ggaaggtacc tttccaggat ttccagaact
    15301 ttggcttcct cttccggcgt caaattcagt tttgacataa cggctctcct ttcttgaaat
    15361 tcggtcgccg acacttccgg cggcgctttg cttttccggc tgcttcaccg gattaaacga
    15421 aaaaaaccgg catcaggtaa gtttgatcac cctggggttt acgagccttt caaaatcctg
    15481 gtaggacatc cgcaccagtt ccgaatggga gcaggcgttg aaaaagatat cctcatcttc
    15541 ccgtaaatgc tcgtccacgt aaacctccat gtcgtataga ttcccgaaag gaggcatggc
    15601 gccgacttcg caatccggaa acagtccctt gaactggccc tccgtggcaa gatggatctc
    15661 ctgatcgccg acggccttgc gaaagtggtc aaagttgatg gagcgattgg ccggcagaac
    15721 cgccatggcc atctttccct ccatcacaac aatgacggtc tttgccacca tgcccccctt
    15781 gatatgcagg gactgggcaa tttcccgggc cgtataggcc ggtgaatgag caattttgat
    15841 gtacttaacg gcctgactgt ccaaatattc tttcaatttt cttgcgggca taaggaacct
    15901 cctttcgatt taatttttgt tcctggctgg atcaatcctt tcttcattta ttctctttcc
    15961 cacaaaacgt tgttttcccg tgatttcttc cgtctgcgag caatgcggtg agcgaatgaa
    16021 agtgaatgac agcaggggct tgatgtttga gaaaggaggc ttccggaatc ggcgcgcttg
    16081 tggactttta aaaagcactg cctggtgtga gtttaatgta gccccggaaa acgtgaatgt
    16141 caatagcaaa ccagttgggg caacagtccg ttactctttg acatttccag atacaaatca
    16201 aaagattgtt gacaatccct ttcagaaacc ttccaggaac tgcttgactt atcatccttc
    16261 gtttcctata cgtgatctca gacaatggcc ggacagaaag tttattcagc cggtttggac
    16321 cgccaattca atctgccata tgattgaagg gcaagcgtcc gatccggacg gcacaggagc
    16381 catacagaaa ttggacaatc tttccaaccg caaaacggct gtcgccattt tccgggcggc
    16441 gcttgcgtcc gtcgatccct gccggctggt ccaaaaacac attccggacg ttctggacgc
    16501 gtatcggcgg ggaaacggtc aaagactgat gctggcggcc ttcggcaagg ccgcttttgc
    16561 catgacgcgg gcgcttgccg aaagccccgc tggagagctg atcacacggg ggaccgtcat
    16621 cacgaaatac ggtcatgtgc ctgggacgct gcccgacccg atcgcggttt atgaagcggg
    16681 acatcccctg cccgatgccg caggcgttgc ggcgacgggc cggctgatgg ccatgctgga
    16741 gaaccccgac ccgcagacgc ttttggtgtg cctcatttcc ggcggcggct ccgcgctttt
    16801 ggtggcgccc gcagacggcc tcaccctggc cgaaaagcag gccacgacgc aatgtctgct
    16861 tgcggcgggg gcggacatcg gcgagctcaa cgcggtgcgc aagcacattt ccggaatcaa
    16921 gggcggtcaa ctggccgcgc tggcccgtcc ggcgcgggtg ctgtcgctga ttttatccga
    16981 tgtcatcggc gatccgctcc acgtgatcgc ttcggggccg acggcgccgg acaataccac
    17041 ctatgccgat gccctggccg tcatcgggaa atacgggctt gaagaaagaa tcccgaactc
    17101 ggtttaccaa agattgatcc agggccggga aggaaccgtt ccggagacgc ccgcaaaaga
    17161 cgatccggtg tttgccgatg tcgccaacct gatcatcggc agcaacaaac tggctataga
    17221 agccgcggcc gcaaaagcaa ccgcggcagg ctttgaaacc gttgtcctca cgaccgaact
    17281 ccggggcgaa gccagggaag ccgcccgatg gctcgcgcaa caggcactcg cattccaaaa
    17341 ccagcaaccg gccggcaaaa agctctgcct tgtttccggc ggcgagacga ccgttaccgt
    17401 aacgggaacg ggaaagggcg gcagaaatac cgaactggcg ctggcctttg ccgaagaaat
    17461 cgacggccgg ccgggcatca cgcttctttc tgcggggacg gacggcacgg acggaccgac
    17521 ggacgcggcg ggcgccgtcg ttgacgggca aaccattgcg cgggccaacg cccggggcct
    17581 ctcctccgac gcttttttat cgcgcaacga ttcttatacc tttttcaaaa aaacggatga
    17641 actgctgata accggatcaa ccggcaccaa cgtcatggac atccaggtgg tcttgctgac
    17701 ctgacccctt taaggagaaa gaacaatgac caccatcgtc attttggcaa tagctgtggt
    17761 tcttcttggt gggcttctgt ttgccgaaaa aaaggaaagc ctcaagggga tcctcctgac
    17821 gaaacccttt ttgtcggggc tttgcatcgt aacagccttg ttgcagccct atgccgcagg
    17881 cccctatttc tatctcatcc tggcaggtct tgtcctttgc ttcgcaggcg acatctgtct
    17941 tgcctttttc tttaaccgca aggtgtttgc ggccggcctt gcgtttttcc tcacggggca
    18001 tgtcgcctac atcctggcgt ttttcatgac ctccggcgtc aacgccggca cagcggtgtc
    18061 cctcgcagca gttgccgtcg tctccaccta tgtatttatc agactgcgtc cgcacctcgg
    18121 tcccatgatg ggtccggtca tcgcctatat tgccgtcatc tccattatgg tggtgggagc
    18181 ggcctctctt gccgtccgcc aggacttgtc ctttcaagcc cggacgctag tgatggcggg
    18241 atcgatcttc ttctacgctt ccgacctgtt tgtggcaagg cacaggtttg tgaaaaaaag
    18301 catcttcaac cgttatgccg gtttgccgat gtactatgcc gcccagttca cgctggtctt
    18361 ttcagtggga atgatcccct aagcgaggat ctgatgaagg cggaaatacg atatcatcat
    18421 actcttctcg acattgaatt tcccgacaca agccggatct atcgctcctg cttcgagcct
    18481 cccgcggcgg ttgaatcaga acttgtcctg caggccatca gaaatccgct ctgctcggaa
    18541 agtcttgtgg attccatcaa aacaagaccc cgcaacagtg tgatgatcgc cgtatcggac
    18601 attacaaggc cgatccccta ccggacgttt ttaccggtcc tgcttgatga aatcgagtca
    18661 accggaatcg atcgaaaaca gatcaccctt ctgatcgcca ccggcatgca caggcccagc
    18721 acaaaagaag aaagggcttt catgtttggt gatgatattg ccggcagata taaaattctg
    18781 gatcatgatg ccgaaggaga catggtcacc ctttccggca caagctgttc cggtgctccg
    18841 gcaaaactca acagagactt tgcgaaggcg gattataaaa tcgtcatcgg gcttgtcgag
    18901 cctcatttta tggcgggttt ttcaggcggg cgcaaaacgc tttgtcccgg cctctcctcc
    18961 atggaaacca tcaagcactt tcacgggtat gaatttctct ctcatcccaa ggccggcatt
    19021 gccctgctgg atgggaaccc ctgccatgaa gaggcgctgt ctgtggccaa actggtcgat
    19081 gttgatttca gcctccaact tgtggtgaac catgcaaaac ggatcgtcaa ggcttttggc
    19141 ggagatcttt ttgagacgca tgccagtgcg tgccattacg taaaaggaaa ggcctgtcca
    19201 atcgttgagg cagaggcgga tgtcgtggtg accggctgcg ggggctatcc cctggatgcc
    19261 accttttatc aatgcaccaa agcgctggtg acagccctcc cctgtgtaaa aaaagggggg
    19321 atgatcattg ccgcgggagg atgcgtggaa ggcgtcggct cacagacgta tgaaaacatg
    19381 ctccgcaagt acagcaacga ttttccccgc ttcatccgca acatcagcag atcgaccgac
    19441 atcgtaaaag atcagtggca gatacagatg caggccagag tctatgagaa aaccgggccg
    19501 gataaccttt atttcttttc ccatggaatt gcttccggcg ccggcgcatt tctgggagca
    19561 aagatcatgg aggccggttt ggagagaatt accggcgaac tgcaaaaagc tgtcgacgct
    19621 ctggccaggc agggatattc ttttgccgta ataccggaag gaccttattg cgcgccgctg
    19681 agcaaaggat tggtttagcg gagtcacgca tcgcccgatc aacgtcatgg tctttgctct
    19741 ttttacaaat gaagcaaaaa tgaaattgga agatcgtcac cgtcgtccgc gttttcaaac
    19801 agaacgaaaa taattcttga caaaaacatc ttaaaagaat aattactagt tcaacgatag
    19861 taattatagt aatttataag gtattgaata tatgaaactt tccactaggt cccgctacgg
    19921 attgagattg atgctggcac tggccagaaa ttatggaaaa ggcaatactt ttctgaagga
    19981 gattgcccgt gaagagggta tttcggagaa ataccttagt ctcatcatca tccctctgaa
    20041 aggagtcgga ctggtcaatt ccgtgagagg ggcatccggc ggctatacac tggccaggga
    20101 accggcccag ataacgctga aggatattat ggacgttctc gagggagact gcctggtcga
    20161 ttgtctgaaa gatcccctgt tctgctccag ggtaaacatc tgcgcgagtc acgatatttg
    20221 ggcgatgttg agcggcagga tatcagaaac attgagcggg ataacgctgg aacagcttgt
    20281 cacgatgaat aaagataaat tggaaaagtc agtaagtcca tctatataat tctttacggt
    20341 gagcgaactc aaggatgaat aaaaaaattt tagtggccat gagcggcggc gttgattcat
    20401 ccgtcgcggc attgttgctg aaaaaagaag gatacgatgt atccggagta accatgtgtt
    20461 taggcattgc gacagacgac agcgcaaaat gctgcggagc atccgcgctt gacgacgcca
    20521 aaagggtctg cgaccggctg caaattcccc attacgtttt cgattacgcc tcccagttgg
    20581 aaaagaaggt catcgcccac tttatcagcg aatatcaaaa ggggagaacg ccgaacccct
    20641 gtgttgaatg caaccgccat ctgaaattcg gcacgctttt ggacaaggcg ctgtcgctgg
    20701 cctttgattt tctggccacg ggccattacg cagccatcgg gaaaaacgaa aacggttatt
    20761 ttctcgctag gcccaaggac aaaaaaaaag accagaccta ttttctctat tcgatccctt
    20821 atcagaggct gggcgatatc ttattccctc tcgctcgttt cacgaaagag gaggtgcggg
    20881 atttagcgaa agaatattct cttcccgtag cggcaaagca ggagagccag gatatttgtt
    20941 ttgtcacgca gaaaaattct caggaattcc tttcggagcg ggtgcgggaa tgcaagccgg
    21001 gtcccatcgt ggatcttcat gggaaattgc tgggccggca tcgcgggatt atctttcaca
    21061 cgatcggcca gcgggcaggc ctgggaatca gccatccgac gccgctttat gtattatcca
    21121 tcgattcggg caggaacctc atcgtcgtcg gagagaaaaa agatttaatg gcaaatgggc
    21181 ttgtggcttc tgatgtaaat atcctggcgg aaaactggcc gaaagaagta cacgcgaaaa
    21241 tcagataccg gaaaaaaggc gcgttgtgca gggtgacaaa agaaaaggat aaattaaaag
    21301 tagtctttgc cgagggacaa gaggcgatca cgcccggaca gtctgttgtt ttttatgatc
    21361 atgaccgggt tctcggcggc ggaatcattg atgaggtaat gcatggaatt tattaaagaa
    21421 atagaacggc tgaaaaaaga aagaaatgcc gtcattctgg cgcacaacta tcaaattccc
    21481 gaagtacagg ctattgccga ttatgttggt gactctttag gcttaagcat tcaagcgtct
    21541 aaaaccaaag ccgatgtcat tgttttctgc ggcgttcatt tcatggcgga aacggcaaag
    21601 atactgtctc ccgaaaagaa ggtgctgctt cccgatccgg aatccggttg tccgatggcg
    21661 gacatgatta ccgccgatga actgcgcaaa ttaaaaactg agcatccgca ggcagccgtt
    21721 ctttgttatg tcaacacctc cgcggaagtc aaagccgagt gcgatatgtg ctgcacatcg
    21781 gccaatgccg tcggcatcgt tgccgagcac atgaaaggtg ctgaggaaat catctttgtc
    21841 ccggacaaat atctgggaac ctacgttgcc ggaaaaaccg ggcgggattt cattttgtgg
    21901 cagggatact gccccattca cgcgcggata ctgcccgaag acgtggaaag gcaaaaagag
    21961 aagcatcccc atgcggaggt tctggttcat ccggaatgta cgccggcatt aacggcgatt
    22021 gcgggcaagg tgctttccac ggaaggtatg tgccgccggg cggccaaatc atcgaacaca
    22081 gaatttatta tcgcgacgga agtgggtatc ctgcgccgca tggcaaaaga aaatccggag
    22141 aagaaattct accccgcttc agaacaggcc ctatgcccca acatgaagcg aacgaccctg
    22201 gagaaagtcc tgtggtcttt gcaggatttg aaacacgagg ttgacgtccc ggcagacatt
    22261 atgatccggg cgcgcagaag catcgaagga atgctttcgt gtcaaccgca aaattgaatg
    22321 cttaataaaa cggaaaggaa actaaaatga atatcgaaaa ttctttgaaa atagaaaccc
    22381 tggcgctgca cggcggacag gaacccgacc cgacaacagg ttcgcgtgcc gtgcccattt
    22441 atcagacaac ctcctatcag ttcaaggata cggatcacgc cgccaatcta ttcgctctca
    22501 aggagttcgg caatatttac acgcggctca tgaatcccac caccgatgtg ctggagaaaa
    22561 ggctcgcgct gctcgacggc ggtgtcggcg cgctggcggt cgcgagcggc caggcggcca
    22621 tcacgctggc gcttttgaat atcgcccagg ccggtgatga aattgtctcg gcggataatc
    22681 tctatggcgg aacgtacaat ctgtttcatc acaccttccc ccgtctggga ataaaggtga
    22741 attttgtgaa gtccaatgat ttgaaggcgt tgcaggcggc catcacaccg aagaccaaag
    22801 ccgtctacgc ggaatccatc ggcaatccca aactggatgt ggccgatctc tacggcatcg
    22861 ccgccgtcgc gcataaaaac ggcattccgt ttgttctgga caataccgtt tctccctatc
    22921 tgctgcgccc gctggatttc ggcgtggata tcgtcgtgta ttcggcgacg aaatttatcg
    22981 gcggacacgg cacgtcgctg ggcggcgtga tcatcgattc gggaaaattc gactggacca
    23041 acggcagatt tccgctgatc tccgggccgg atgccagcta ccacggaatt gattttgtcg
    23101 aagcgttgaa gcccaacggc aacatcgcct atatcatcaa ggcgcgggtg acgctgctgc
    23161 gcgatctggg accggccata tcgccgttca attccttcct gcttcttcag ggactggaga
    23221 cccttcatct gcgtttgccg agacacgccg aaaacgctct ggcgacggcg caatacttga
    23281 agacacatcc ccgggtcgcc tgggtgaatt atccgggact ggaagacagc cctgaaaagg
    23341 aacgcgccca aaaatacctg tccaagggcg cgggcgcgat tttgggtttc ggcatcaggg
    23401 gcggcgcggc agccggaaag aaatttattg aatcgctgcg tctgatttct catctggcca
    23461 atgtgggcga cgccaaaacg ctggcgattc atccggcaac aaccacgcat cagcagttat
    23521 ccccggaaga gcaattcgcc accggcgtga cgcctgattt tatcaggctc tccgtcggcc
    23581 tggaacacat tgacgatatt ctggcggaca ttgagcaggc tttacaaaaa gcagaatcct
    23641 gacaaacaat taatgaggat cataaaatga ccaatttata tgacgatatc acaaagacgg
    23701 tgggtagaac gcccctggtg cgtctcaacc gcatcaccaa agatttaccg gctactttcc
    23761 tggtcaagct ggagtcgttc aatccgctct ccagcgtcaa ggaccgcatc ggcgtcgcca
    23821 tgattgaaga tgccgaagcc gggggattat tgaataaaga ctccgtcatt atcgaaccga
    23881 ccagcggcaa taccggcatc gctctggctt ttgtctgcgc cgcgaaaggt taccggctga
    23941 ttctcaccat gccggagacg atgagcatgg agcggcggca gttgctgaac atcctgggcg
    24001 cggaacttgt cctgaccgaa ggcgcaaaag gcatgagggg cgcggtggaa aaggccgaag
    24061 agctggccaa agccacgccg ggcagtttta tgccgcagca gtttgccaat ccggccaatc
    24121 ccgccgtgca ccgcagaacc accgctgaag aaatttggaa ggacacggaa ggcggaattg
    24181 acttttttat ttccggcatc ggcaccggcg gcaccatcac cggcgtggga gaaattctca
    24241 aacagaaaaa gccgtccgta aaaatcatcg gcgtcgaacc ggctgattcg ccggtgctgt
    24301 cgggcgggaa gcccgggccg cacaagatcc aggggatcgg cgcgggcttt gttcccgatg
    24361 tgttgaaccg gaaaatcatc gacgagatga ttgccgtcaa gcatgaagat gccggtctga
    24421 cggccaggcg tctggcgaag gaagaaggca ttctcgcggg catatccagc ggcgcggctc
    24481 tttgggccgc cctgcaggcc ggggggaagg aacaagccaa aggcaagacg attgtcgttt
    24541 tactaccgga tacgggagaa cgttatttat cgacatggtt gtttcatgag cgataaaaat
    24601 acttattata gaataccccc cttcctctca tccctctacc ctgaagggcg ccgtggccgc
    24661 caagggaggg agattccctc tccctcgagg tgagaggatg atcgtgaggg tgaggaacca
    24721 ctatgaagaa agcgaaacac gaaaaaacaa atattcattc cgtcggtgcc gtggaaacta
    24781 aaactttcac ttttgccgaa cagctttatc tggcaagcgg agaaaaactg gggcctgtga
    24841 cgcttgctta cgaaacgtat ggaagcttga acaaagagaa atcaaacgcg attctgattt
    24901 tgcacgctct gtccggcgac gctcacgcgg cgggagtcca tgccggagac aacgacacag
    24961 gctggtggga ggaactgatc ggtcccggca aagcgttcga tacgcaccgg tattttatca
    25021 tctgctcgaa tgttgtcggc ggctgcaaag gatccaccgg gccatcttcc ctgaacccgg
    25081 caacgggcaa accttacgcc ctggattttc ccttcatcac ggtggccgat atggtggaag
    25141 cgcaaaggca tcttatcgat catctgggca ttgacaaact cctctgcgtg gcagggggtt
    25201 ccatgggcgg catgcaggtt ctgcaatggg tttcctctta tccggaacgc gtgaaatcgg
    25261 ccattcccat cgccacgacg ctgaagcatt ccccccagca gattgctttc aacgaggtca
    25321 tccggcagtc catcatggcg gaccccgcct ggcgtgaagg aagctattac gaattcgggc
    25381 agccggagaa gggattgtcg gtggcgcgca tgatcggcca tatcaccttc atgagcgaac
    25441 actcgatgga ggaaaagttt tcccggcgtt taaagagcga taggttcagc ttcggattcg
    25501 acgcagattt cgaagtggaa gggtatctgc gttatcgcgg cgctaatttt gtcaagcgct
    25561 tcgatgccaa ttcctatctc tacattacca aagcgataga ttattttgat ctgtccggcg
    25621 gcaaattcct tccgcacggc aagaccgttg acacaaggtt tctgattatt tctttcaagt
    25681 cggactggct atatcctccc catcagtcac aggaaatcgt cagggcgctg aaaagaaaac
    25741 acgtcgatgc cacgtactgc gaattgacct caacctatgg acatgacgct tttcttattg
    25801 aggtggagga acagacaaca ctgatcaaac attttctgga taagacattt aacggatatc
    25861 tggtaatggg caatgatgga atttaacact caattggatc accaaatcat ctattcgatt
    25921 gttaaatcgg attcgcgcgt tctggatctg ggctgcggtg aaggtgaact tttgtatccc
    25981 ctggttcggg ataagcatat ccgggcacag ggaatcgaac ttaacgacca gtccattcag
    26041 gaatgcgtca agaagggttt gagtgtcttt cacgatgata ttgaaagcgg tttgcgggaa
    26101 tttccggata atgcattcga ttatgtcatt atgaatcaga gcatgcagga agtcaaaaaa
    26161 gtggattgcg tgatacagga agcgttgcgc atcggcacac aggttattgt cggtttcccc
    26221 aattttgccc ataggacatc gcggttcagt ctgtttttcc ggggaaaatc tccggtgacg
    26281 aaatcgctgc cttatctctg gtatgatacg cccaatgtgc gttttttgag cattacggat
    26341 ttcagggatt tttgcgttga aaaaaatatc cggattgtgg aagaacacta cctgggagaa
    26401 aagaccgtgg tgcgcttttt cccgaatctt tttgccttaa acgccatctt tgtattgaca
    26461 aaaaaagacg aggcggcgtt gaaatcaggc gggaaggggt atagattagt cctgtaaatt
    26521 ttttaggaag ccgaacactt tgattttaac aaataattca gaacgaagga gtgtctatgt
    26581 gggactatac cgataaagta aaagagcact ttctgaatcc gcgcaacgtg ggtgaaatcg
    26641 aaaatccgga cggcgtggcc gaggtcggat cgctcgcctg cggcgacgct ctgaagctga
    26701 cctttaagct ggacgaaaac aaacgcatcc gggatgccaa gtttaaaacc ttcggctgcg
    26761 ccagtgcgat tgcctcttca tcggcgctga cggaaatgat caaggggatg acggtggatg
    26821 aagcgctcaa ggtcaccaac caggacattg cccgatacct gggcggactg ccggacgaaa
    26881 aaatgcactg ctcggtgatg ggcaagcagg cgctggaaaa ggcaatcgag aattaccgcg
    26941 gcgctccgtc gctggaaccg ggagcaaaaa ttatctgcga atgtttcggc gtcaccgaca
    27001 aggaaatcga gcgggccatc cgcgaaaaca atcttgccac ggtggaagat gtgaccaatt
    27061 acaccaaggc cggcggcggc tgcggcaatt gtcaggatgc cattgcccag attctggagc
    27121 gggtgaaggc ggaggcaaag accgaggtta agccgaagct gacgaacatt caaaaaataa
    27181 ggctgatcga agaaaccatc gagcgggaga tccgtccatc gttgaagcat gacggcggcg
    27241 atattgaaat catcgatatt atcggtaatc gcgtgatcgt ggccctgcgc ggcgcctgcg
    27301 caacctgcaa ggcgtcgaat atcacgctga aggattttgt cgaacagcgg ctcagggaac
    27361 tggttgtacc ggacattacc gttgaggagg cttccaaatg aaaaccatct atctagacaa
    27421 taatgcaaca acaaaagttg ccgatgaagt tctggaagcg atgcttcctt atttcaacca
    27481 attctatggg aacccatcca gcatgcacac cttcggcggt cagatcggtc agcgcatccg
    27541 cgacgcgcgc gagcaggtgg ccgcgttact gggcgcattg cccgaagaaa ttgttttcac
    27601 cagctgcgga acagaaagtg ataataccgc catttattct gcgctgctga ccaggcccga
    27661 taaaaaacac atcgtcatca gccgcgtcga acacccggcc gtccgcgcgc tctgcacgca
    27721 tttgagcgca caaggctata aaatcacgga actgcccgtg gataaaaacg ggatgctcga
    27781 tctggaacat ctggaaaaaa gcctgacgcc ggataccgcg attgtcagtc tgatgtgggc
    27841 caataatgaa accggcgtca tctttccggt ggaagaggcg gctaaactaa cccatgacaa
    27901 gggcgttctc tttcataccg acgcggtcca ggccgtgggc aagattccca ttaacatgaa
    27961 aaataatgtc attgacatgc tgtccatctc cggccacaag ctgcacgctc cgaagggaat
    28021 cggcgttctc tatatccggc gcggcaccaa attttctccg tttctcatcg gcggccatca
    28081 ggaaaaggga cgccgcggcg gcacggaaaa tacggcgggc attatcggac tgggcagggc
    28141 ctgtcaactg gccgcaaatc atatcctcac ggaaaacgtg cacgttaaac aactccgcga
    28201 caagctggag cgcgaactct tgaagaagat tccgcaaagc cgcgtcaacg gcgatattca
    28261 gaaccgtcta cccaacacga ccaacatcag ctttgagtat gtggaaggtg aagccattct
    28321 gcttttgatg aacgaactgg gcatttgcgc ctcgtccggc tcggcatgca catccggctc
    28381 attgcagcct tcacatgttt tgcgcgcaat gggcgtgccc ttcacgatgg cgcacggctc
    28441 catccgcttc agtttgagcg tgtataatac ggaagaagaa gtggattttg tcattgaaaa
    28501 aatgccgccc atcatcgagc ggctgcgggg catgtccccc ttctggagtc aggcgcaaca
    28561 agcctgcaca aatcaatagg agcggcggat gatgaagcgt ttgaacaacg gcggcaaatg
    28621 cagggaagaa atcaagtcag cgaagcattt tcgcaaggaa gtgccggatg tcgttgacgg
    28681 gcttgttcat tcgtgcaaca gcgccggatg cttcgatcat gtgagcgccg agccgattcc
    28741 ccatcgtgag gccatcatca acatccttca tcgtctgtct ttgattcttt acccagggta
    28801 ttttatccgg actcgtttgg attcaacgaa tcttgaatat tatctgggcg aacaggtgac
    28861 ggctttgtat gaaacgctct ccgagcagat tgtcctggcc attcgccacg actgtatccg
    28921 ccataacaag tcctgcgtgc attgcgagcc gctgggacat cagatgacgc tggcgtttct
    28981 gcgcaagctt cccacgttgc gcttcatgct ggccaaggat atccgcgcct ccctggaagg
    29041 agatccggcg gcaaaaggat atgatgatat tatcttcagc tacccgggca tccgggcgat
    29101 cacgatttac cgggtcgcgc atgaacttta tcatctgaac attccgctga tcccccggat
    29161 tatgacggaa tacgcgcaca gccgcaccgg catagacatt catcccggcg cgcacatcgg
    29221 cgagagcttc ttcattgatc acggcaccgg cgtggttatc ggcgaatcct gcgtcatcgg
    29281 caaccgtgtg cgcatctatc agggcgtcac gctgggcgcg atttccctgt ccaagtcgga
    29341 agtcaagaaa ctaagatcga aaaaacgcca tccatccata gaagatgacg tcatcattta
    29401 cgccaatgcc acaatcctgg gcggggcaac cgtcgtcggc gcgcgctcca tcatcggcgg
    29461 caacgtgtgg ctcacgcatt ccgtcccgcc ggataccgag gtctttgtca ataaacagga
    29521 tctgattttc ggagcgaagc aatacaaaaa acaggccgcc aaacagaagc tgcgccaggt
    29581 gaatcagaat catctgatca ttaaataaca gatgttaaaa acatgaatga gaaactgttt
    29641 tcaacttttg atatcaaaga aattatttcc gacagcggcg tcatacgttt gcgcgattcc
    29701 gaaaaagacg ttatcggcga gatttcgctc aagattttta tcaacgggac ggaatatgct
    29761 tcgctgttgt gtctgaatca actcacggaa gaactggcgc tgggcttcct ccacagcgaa
    29821 ggcgtgattg attccatcgg cgatgttgcc tctatttcct ctaacgagcg tctgtttgcc
    29881 gtgatgatca atcttgcgga aggcaaatcc gtggagaagt gcgagagtct gcgcagtgtc
    29941 acctccggct gcggtaaatg cttcacctac atcaatccgc tcaagcatga taagtttgcc
    30001 accctgtccg gtgaaaataa attcttgtta agcaacatca tacagtccat gaaggacttc
    30061 gagcgtcgtt ccgaaatata taaaaccgtc ggcggggtcc acagcgtctt gttccagcat
    30121 gacgagtatt ccgtattcaa cgaagacatc ggacggcaca actgcttcga caaaatcaac
    30181 ggcatactgg tgaaagaaaa aaagatgcat cttgtcgggg aaggcttact gtttgtcagc
    30241 ggcagggtgt cttcggaaat catcaccaag gtcatccgca tgggcgtgcc ggtgctcgtt
    30301 tcgagggcca cgccgacatc cgcggcggtg aatctggccc gggaatacaa tgtcacgctg
    30361 ctgggttacg taagaaaaga ttccggattt gtctattcgg gacaggagcg tctcatctaa
    30421 gggaaaaaca tgaaaacaat aaagggaaaa attctagcgg tgaatcgttc gcacaagaaa
    30481 ggcgaaaaga aaagcaatat cgattgcggc ttgtttctgg aaaacctcgg attggaaaac
    30541 gacgcccatg ccgagcccgg catccggcag gtgagtcttt tggccaggga aagcattgag
    30601 aaaatcagag acaaaggcct tgatgtgcaa tacggtgatt tcgccgaaaa tctgacgacg
    30661 caaggcattg atctgccgtc gctgcccgtc ggcgccaaac tgaaagtggg cgaaaaggta
    30721 ttgctggagg tttcgcagat cggcaagatc tgccacaacc gctgcaatat cttttatacc
    30781 gtcggcgatt gcgtcatgcc ccgcgaagga atatttgcca aggtcatcac gggaggcgaa
    30841 ataaagatct tggatgagat caccgtcatc gattgaaaaa ggggtcaaac ggcgtcggtt
    30901 gaatataatg cttccgcgat tttttcctcc atcgacgcaa ggttgaattt tacatttcgg
    30961 gaagtggttt tccacgaccg acatggcggc cgcgccctac ggtacgccga tgaaatattc
    31021 cggagcaatc aaccacctcg cagcaagctg tcgaggcatg aaatgaacat cattatatcg
    31081 cagcaagctg cggagaatga acgctcgtcc cgcttaagcg ggattcaact gagacggtgt
    31141 gtgtgaaacc gggggaaaac gctcccccgg tttcgcgggt atcatttact tcaacctttc
    31201 ttcggagaga tgcaggtggt ccgtctcgat ttccaccagc gctgcaaaga tctgtcgaag
    31261 tctgggatga tccgattcgg aggccgcctt gcggtagaag tcgatggccc ttgtttcccg
    31321 ggcgtgggat tccttgagat tctcgacatt cggcgtatag caggactctt ttcccggagg
    31381 cacgcttccc agcttgagga tttttctcca gatggacgcg tgctccgctt caatcttgcc
    31441 cagtgccttg aacagaatct tcccttcggg atcatcggtt tgattcgcgg cgcacatgta
    31501 aaaggctgaa ttgctgattt ccaccttgag cgcgtgttcg gctttggcct gatccttcac
    31561 gctcagcgac acatcaaaat ttacgaccgc gtctttggcc tctacaatat actccatgtg
    31621 cgcgccgcag aacgggcagt tcgccggagg actggcgccg atatacgcat cgccgcatat
    31681 cgtgcatcgg tataacttgg ccatgtcttt tcctttcggc aaaaatttgc ctgctttctc
    31741 ttatgctttc cacagaccgt gcagattaca gtattcccgg gctgtaatcg tggcggcttc
    31801 gatggggaaa aacgcttccg gcgcctgtcc gggcgccaga tactgacgat aggctttacc
    31861 gtccgcgatg acttcgatcc attcgatgta atgcttttct tccatcgggt gagccacgct
    31921 gcccactttt accttgaagc cgcccgccat tttctcaatc gccggaacat gcttttcctt
    31981 tgaggcgtca accgtatttt ccgtcaaaag cttcatgggc tgattgcagc agacgagctc
    32041 gcccttgcct tcgtgaagca cttccacgat atgtccgcac acctcacatt taaagacttc
    32101 gtttcttttg gccatgatga acctcctttg ttggaatgat taatcgacct ttttaaacat
    32161 tttacctttg gcgccgcaga ccggacaggt ttccggcgct tctttttccg cggtatagcc
    32221 gcagaccgga cagatataat agctgtagga ttcttttgcg ccggccagat tgtccagcat
    32281 gcgctgataa agccctgcat gaatcttttc cacttcgttg gcgaaaacaa aagagcgctc
    32341 cgcctccttg tggccttcca ccttggccgc ttcgatcatc gccggataca tgattttgaa
    32401 ttcatgcgtt tctccggcaa ccgcttcctg aaggttttct tttgtggagc ggatgccctt
    32461 taatgccttc aaatgggcgt gtgcgtgaat cgtttccgcc tccgcagctg cccggaataa
    32521 tcttgcggcc tgggcaaatc cctcctggtc ggccttagcc gcgaacgcca gatattttcg
    32581 gttcgcctgt gattctcctg caaaagcttc ctttaaagca tcttctgttt tcgacattct
    32641 tcatttcctc ctttctcaat ccggaatgat tcctaataaa atataaaggc ctctcctcct
    32701 ttttctttta ccttccgtgt atgtcctgcg agactgctgg atcaaaagtt cagtcggggc
    32761 gcttcaaaca atcccggcaa tagccgtgaa attcaatccg ggtgtcggta acgacaaatc
    32821 ccgattttcg gacaatattt tttggatcga gcggcgcggg gacgtggtag tccatcactt
    32881 ttccgcaccg tttgcagatg agattcacat gatcggagag atccacgtca tatcggttct
    32941 ccagacggtc aaattgcatt tgccggatca gtccgctttc ggaaaattcc ttgagtgtcg
    33001 catacaccgt cgacaggctg acgcgtccgg cttttttccg ggcttcatgg aagatgaggg
    33061 tcgcgccggg atgctcgtag cgattttcca ccagcgcgtc aataattgcc aaacgctgcg
    33121 gcgtaatctt gagcccgttg tcttttaact gaccgatcag gtcttctttg gtattcgttc
    33181 tcatattgga atcattccag tttgagaaac cctaccctta tccggctggt cctgtcaaga
    33241 ggtttttaca aattacagac cctttttctc gaatacaaca aacagtccga tcgttgccgc
    33301 ccagaatatc ggcacaagca cccagcggga aatgcccaga gcgcccggca ggctgatttt
    33361 gccgaagtcc tgccaagcca tgacagtggc ttgaagaaac ggatacaatt cggcaaatac
    33421 cgccgcgccg accagcattc ccgcgatggc aaacaatgca tgtacgcggc cttcgcctac
    33481 ggctgcaacg gaagtgccgg ggcagaatcc cataaccgcc cagccgatgc cgaaaagaat
    33541 cgcgccaatc accacggcgc ccacattcaa cggtttgtgg ctgaaggtga taatccccat
    33601 atccgagaga atgaatatcc ccaccatgcc gaccagaatc gaggaaagca tgaatttgaa
    33661 tattgtcatg tcttttatca aaagcgcgcc aatctgtttt tcaaagcgga tgacccgccc
    33721 tttctgcaac aggaatccga acaccacacc ggtgatcaac cccagaatct gatcaatgct
    33781 catgatgccc tccttctgta aaccagcgcc gccgtcagtg cgccggccgc aaaaaacagc
    33841 gccagcgcca cgaaagaact tgccgacagc tgcatcatac cgctgagtcc gtgtccgctg
    33901 ggacatccgt ccgccagccg cgcgccgatc attgccacaa tgccgccgag aaacgcgccc
    33961 gctcctcttt tggtcatcga ggagccgaag cgttcccgcc agatgggcgg tactttttcc
    34021 acctgaaaac ttttatcggt cagagaggag atcagcgagc ccagaaaaat tccgatgatg
    34081 agcatgaact gccagtccac tttgattttt gtggcggcga aataggcgtt ttgagccaca
    34141 tgctccgaag cgaaaacctt ctccacaaaa ccggcgacgc ggacaaaagt ggtggacgcg
    34201 cccagataat tggttttgcc catcagctct gtcgtggcac acaccgaagc aatcgccaga
    34261 atgccgacca gcgctccggc aaagtaagga ttccatcctc cgtcttttgt tttaagcttc
    34321 atcgttgcct cctttccttc gttgaatctc ttagggttcc attccccgcc gcttgcggcg
    34381 aatggcccat acaaaataga aagcttttga taccccgctg cttgcggcgg ggttgttcat
    34441 ttagccaacg gcggcttgta tcgtatccga tcccggcaag aattgccacg aagtttttcg
    34501 ggtgacggat aagtgggggc gacgggttgc aatttccacc gctccatgat atgagctgca
    34561 c
//
LOCUS       DAOSRM010000010        34507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1389_length_34507_cov_101.502264, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000010 DAOSRM010000000
VERSION     DAOSRM010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 34507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34507
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1389_length_34507_cov_101.502264"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..594
                     /gene="rpsF"
                     /locus_tag="PLA58_02155"
     CDS             124..594
                     /gene="rpsF"
                     /locus_tag="PLA58_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOR61628.1"
                     /translation="MKRYEVIAIVKTDVSEEDLTAIMERSSNIITERKGVIAKAEKWG
                     KRRLAYEIKKQKDGFYFYIDYAGDGSIVAEMERNFKIDDRILKFMTVAKEGAVTREGI
                     DAEIAAAEAKRTQIRIETDNTAAAKEEKAAGDARPARPTFRKAPHREDNTPKGE"
     gene            597..866
                     /gene="rpsR"
                     /locus_tag="PLA58_02160"
     CDS             597..866
                     /gene="rpsR"
                     /locus_tag="PLA58_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOR61629.1"
                     /translation="MAAPNGERPSRPPQRKFFHRKKFCRFCTDSNVKIDYKNPVILNN
                     FVTERGKIMPRRITGNCAYHQRELTVAIKRARTIAIMPFVTAEGR"
     gene            1001..1954
                     /locus_tag="PLA58_02165"
     CDS             1001..1954
                     /locus_tag="PLA58_02165"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOR61630.1"
                     /translation="MNLSFFQNSFLRLLLTITLFLAASLLPFAGLVFLLFLPLVLYVL
                     CFLADPGKTLIAFLASLCVVTILLWALNASASAPALAAAGFAGMLMFWTVQKRFSVEG
                     VVLLPSLLLVGAIALYFIYGGMQLSITPWQLVEKRIQEAVELNIRLYSQFPLNAEEIQ
                     EITNSKPQMISLFTMIFPALCVIAAVFTIWINLLAAGRLLKGRGILPAPLSALSEWSA
                     PAWLVWIFIAAGGMSAVPMAPVRFSGVNLFLVASFIYFLQGLAIVSFFFQHRNISPFF
                     RFLAYFLIAIQQMLMIAIAAVGLFNLWVDFRKYLRKDRTTD"
     gene            1979..2428
                     /gene="rplI"
                     /locus_tag="PLA58_02170"
     CDS             1979..2428
                     /gene="rplI"
                     /locus_tag="PLA58_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOR61631.1"
                     /translation="MKVILIQNVPSLGKAGDLVKVNDGHARNLLIPKGLAVEADHKNI
                     KAFELAKKNILQKTQKEKEGARDLASRLSQVTLTLSRKVGDQHKIFGSVTSKDIEAAL
                     REKGFSVDRKMIVHDEPIKSLGEFKVRIKLHPGVETEIKIIVAGEES"
     gene            2425..3780
                     /gene="dnaB"
                     /locus_tag="PLA58_02175"
     CDS             2425..3780
                     /gene="dnaB"
                     /locus_tag="PLA58_02175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOR61632.1"
                     /translation="MKDIDPSLYKLPPQNIEAEQSILGSILLENSAINAAQEILNKDD
                     FYSEAHRKIFSVIEQLADKNEPVDLITLGNALKDKTLLDSVGGIAYLASLVDNVPSAA
                     NVANYAKIVKEKAILRGLIGSATEIINSCYETDSDVDQVLDKAEHRIFEISENKVRTS
                     FYPIKDIVRESFRAIEDLFTRKELITGVATGFDKIDDMTSGLQASDLIIVAGRPSMGK
                     TAFALNIAQHAAMETQTPVAIFSLEMSKEQLAFRLLASEARVDSQRLRKGFLGETDWP
                     KLTAAAGRLSEAPLYIDDTPAITVLEMKAKSRRLKADRDLGLIVVDYIQLMRAQGNQN
                     SREQEISEISRSLKALAKELKVPVIALSQLNRKVEDRPNRRPQMADLRESGAIEQDAD
                     VIAFIYRDEVYNKSDDNPDKGVAEIIIGKQRNGPTGLVKLAFLDRFTTFENLARPDGP
                     S"
     gene            3831..4337
                     /locus_tag="PLA58_02180"
     CDS             3831..4337
                     /locus_tag="PLA58_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61633.1"
                     /translation="MRPKEVIVNDRMQKGYRYVLTKPAGRDFDPDFKPELTPKQMLEI
                     GVFGGKYMTDCRREFPASWFAKARLSPERHDPKLNCFGVNASKPLSYWVAKGWIHPDD
                     PRGWFQWYCRYYLGRRRPDDERQIKRWSAMQRHISQLTKNCPAGDLSCRRKQRQALLH
                     WAYDSRKM"
     gene            4802..5083
                     /locus_tag="PLA58_02185"
     CDS             4802..5083
                     /locus_tag="PLA58_02185"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOR61634.1"
                     /translation="MIKSFRCRETEKIFRRERSSKFPGDIQQRAFMKLNALDAAVKIE
                     DLRLPPSNRLEALRGKRHGQWSIRINDQWRICFVWQKDHAEKVEIVDYH"
     gene            5095..5397
                     /locus_tag="PLA58_02190"
     CDS             5095..5397
                     /locus_tag="PLA58_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOR61635.1"
                     /translation="MLKKRIKPVHPGDYLKELLDELEISQYRLAHDLGVGTMRINHVV
                     NGKRPVNAELALRLGRYFSQEPRYWMNLQSRYDMDMAEDALSDQVSHEVQPLRMAG"
     gene            complement(5493..7937)
                     /locus_tag="PLA58_02195"
     CDS             complement(5493..7937)
                     /locus_tag="PLA58_02195"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOR61636.1"
                     /translation="MFRNKRSIWTFLLSGLLVLLSAGAALASEADIKLPDLSQVSFMG
                     GALSGMMILNVGLVICIIGLIFGVMQYVQTKNLPAHQAMLDVSQTIWETCKTYLFQQG
                     KFLIGLWILIAICMIYYFGGLSGMPAGEIVIILVCSILGILGSYSVAWFGIRINTVAN
                     SRAAFSSLSGNPLNIVNICLRSGMSVGLLLVSIELFFMIMILGYIPKELVGPCFIGFA
                     IGESLGASALRICGGIFTKIADIGSDLMKIVFHLPEDDPKNPGVIADCTGDNAGDSVG
                     PTADGFETYGVTGVALITFLALALAGNPEMGGKLIIWIFAMRILMILTSLVSYFVNNG
                     ISTAAFAGKKEFNFEHPLTNLVWITSIVSIGVTFAASYFLLNANDLDPKYADLWWALS
                     VIISCGTIAGALIPEFTKIFTSTSSRHCEEVVNASKQGGPSLNILSGFVAGNFSGFWL
                     GMVVVLLMFVSYMVSKNASVMALMPPAFTFAAPVFAFGLVAFGFLGMGPVTIAVDSFG
                     PVSDNAQSIYELSMIESRKDAKDVVKAAYGIAPDFELAKHYLESGDGAGNTFKATAKP
                     VLIGTAVVGATTMVFGIIILLEGLFGQVISKLSLVQPEIILGLIMGGAVIYWFTGAST
                     QAVTTGAYQAVVYIKKNINLDKAEASIQDSKKVVQICTQYAQKGMINIFIVIFFMCLA
                     LSFFNPYFFIGYLIGIAFFGLFQAIFMANAGGCWDNGKKIVEVDLKMKNTPLHEASVV
                     GDTVGDPFKDTSSVSLNPVIKFTTLFGLLATEIAVTMTNQNLKIGLAVVFLLIALIFV
                     YRSFYSMRIASEKLGG"
     gene            complement(7941..8591)
                     /gene="pth"
                     /locus_tag="PLA58_02200"
     CDS             complement(7941..8591)
                     /gene="pth"
                     /locus_tag="PLA58_02200"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOR61637.1"
                     /translation="MRLFSFGKYLFRFQKERGQVVYLVVGLGNPGKRYEFTRHNIGFM
                     VADLLAARWKVDLKQKSFNALWGKGTVEGKNVLLAKPQTFMNLSGTAVKQLQSFFKTE
                     ISNLIVIHDDLDLPYGAVRMKAGGGTAGHKGLASIESNLGTSGFMRVRLGIGKPVDKS
                     RIEGYVLEPFRKEEQVMLPEVIHRAADASAEIVLNGLQKAISKYQTKNIDFLKKEE"
     gene            complement(8619..9296)
                     /locus_tag="PLA58_02205"
     CDS             complement(8619..9296)
                     /locus_tag="PLA58_02205"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOR61638.1"
                     /translation="MEITDLTAQVRTEHKKGPARRLRQQGLVPAIFYGKATGNIPLAV
                     KSSDLFRLHKQKKDHAFIKLLIDDGGKKIEKLSLIKELQIQPLSGKFYHADFYEVDMN
                     KKLAFDVELNFTGKAVGVENGGELQHIKRDVRVLCLPTDLPDHIDVDISALEIGHSLK
                     VKDIVIPEGLTHLDPPDAAVVSVAAVRVAKVESAEAVAAEGAEAAEGAAAAAEPAKEE
                     KKEKEKK"
     gene            complement(9311..10252)
                     /locus_tag="PLA58_02210"
     CDS             complement(9311..10252)
                     /locus_tag="PLA58_02210"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOR61639.1"
                     /translation="MLERIRVFTGNANRELARKICEKLGVPLGNANVTTFSDGETRVE
                     IDENVRGMDVFIIQSTCTPVNVTLMELLIMIDAMKRASADRITAVVPYYGYARQDRKV
                     APRAPISAKLVADLITTAGANRLLSMDLHAGQIQGFFNIPVDNLFATPVLIDYMKRNY
                     REDTVVVSPDTGGVERARAFGKRLGASLAIIDKRREGPNEAQVMNIIGNIKGKRVIIL
                     DDMVDTAGTVVQAAQALADAGALEVSVCCTHPVLSGPAIDRISKSAIREFVVTDTIPL
                     CEKAAQCERIKVLSVSGLLSEAVRRIYYNDSVSSLFI"
     gene            complement(10278..10352)
                     /locus_tag="PLA58_02215"
     tRNA            complement(10278..10352)
                     /locus_tag="PLA58_02215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10318..10320),aa:Gln,seq:ttg)
     gene            complement(10371..11273)
                     /gene="ispE"
                     /locus_tag="PLA58_02220"
     CDS             complement(10371..11273)
                     /gene="ispE"
                     /locus_tag="PLA58_02220"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOR61640.1"
                     /translation="MSKKKAEMRRLAPAKINLFLRILRKRPDGYHDLASVMQQISIFD
                     ELICSLRPEGIALSCPGTDLPTDEKNLVYRAARALFEHTGYPGGVDITLSKQIPSAAG
                     LGGGSSDAAATLLALNDLCSLGLSKDELMKLGVKLGADVSFFIFGQTAFASGIGDRLQ
                     ALENTPKLSMVLINPPFPLSTKTVYENLNFTLTKKENNYSIPRFYALGDVIRELHNDL
                     EAVSMNMHPELADFKQLLINEGALGALMSGSGPTLFGLFADEESAREARSAIEKKVSG
                     DCRVFFAQSLSGKYEFSPDEELPG"
     gene            complement(11254..11649)
                     /locus_tag="PLA58_02225"
     CDS             complement(11254..11649)
                     /locus_tag="PLA58_02225"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOR61641.1"
                     /translation="MAEETKGEGFVIRDRRFSEEPDANAKEEPASVKAEEPKGQAGKT
                     PAEPDEYPPVNFTNFVISLSTSALFHFGDFPEVEGGKAEKNLPAAKQTIDILDMLQKK
                     TAGNLDKNETNLIEGVLYELKMRYVKEKG"
     gene            complement(11732..14320)
                     /gene="clpB"
                     /locus_tag="PLA58_02230"
     CDS             complement(11732..14320)
                     /gene="clpB"
                     /locus_tag="PLA58_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOR61642.1"
                     /translation="MRFDKFTLKVQEALQDAQTMAASQGHQAIEPEHLLVCFLQEPDS
                     IVSAVLTKLGASPQKIERELDAYLKKQPRVGGSGAGQVYLGGRLNRLFDQAMTEAAQL
                     RDEYVSAEHVLVAMSEEKEGQAGKTLRQNGVTRDNIFKALVDIRGNQRVTDPNPEGKY
                     QALERYAKDFNELARTGKFDPVIGRDDEIRRIMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVNGDVPENLKDKRVIGLDVGALIAGAKYRGEFEERLKAVLKEVTSAEGEII
                     LFIDEIHTVVGAGASEGSMDASNMLKPALARGELRCVGATTLNEYRKYIEKDAALERR
                     FQPVLVKEPTVEDTIAILRGLKERYEVHHGVRIKDSAIVAAATLSHRYITDRFLPDKA
                     VDLIDEAASRLRIELDSMPSEIDAIDRRIIQLEIEKQSLKKDTDKAATERREKIEKEL
                     AELKSEMDRMKLHWSSEKDIIKKIQQIKSRMEALKTEEAGAQREGNLARAAEIRYGKM
                     VELARELETEQAKLSDVQKNNQMLKEEVDAEDVAEVVSNWTGIPLSRMMESDVQKLIH
                     MDERLRMRVIGQDDGIEAVSAALRRARSGLQDPNRPIGSFIFLGPTGVGKTELARALA
                     EFMFDNEQAMIRIDMSEYMEKHAVARLIGAPPGYVGYDEGGYLTEAVRRKPYSVLLFD
                     EIEKAHPDVFNILLQILDDGRLTDGHGRTVDFKNTVVIMTSNVGSGWIQDPSLSEEEK
                     KNRSMEALRATFKPEFLNRIDDIIMFHGLTLDDIYRIVGIQLELIDRRLKERKLSIEL
                     TDAAKKYIAEEGYSPVYGARPLKRSLQKLVLDPLAMDILAGKFAEGDHIVIGVSKGAI
                     TFSKKG"
     gene            complement(14334..15206)
                     /locus_tag="PLA58_02235"
     CDS             complement(14334..15206)
                     /locus_tag="PLA58_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOR61643.1"
                     /translation="MKSVNKTMVESAIKLAKEVKAGCVLLCVDVRGELAELSEDERKS
                     VRFVFVMRESEELPEKLLPTAKKLELPDVNLTRVGKIKIAIAKGIVSGLFKKGDRIVC
                     LSGVPKFGYADSIFFIDVGREFEILTSDDISDVVDSVQPEVFNAALNIACELAAQGRE
                     TRKVGTIFVLGDDEKVMQLSRQMIINPFKGYSEEQRNILNPELEETIKELSAIDGAFI
                     IKANGAIVTAGRHLSAALDSKDFPSGLGSRHIAAAGITNVTNAVAMVISQSSGNVSIF
                     KNGKLFVTIEKPVE"
     gene            15304..16593
                     /locus_tag="PLA58_02240"
     CDS             15304..16593
                     /locus_tag="PLA58_02240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HOR61644.1"
                     /translation="MLDRQWTLRYFNKPCFFIEEASTLKLPIFTGTLDLYIAEINRFP
                     ILTPEEEFKLAMRLKKDNDMEAAEKLIVSNLRFVVKIAHEYRSYGFKLADLIQEGNIG
                     LIHAVKKFDPEKGYRLISYAVWWIRAYIQNYLIKSWSIVKIGTTQAQRKLFFKLNQAR
                     KQLETISKKNPEFSEIADSLGVKGSEVAEMDLRMGARDLSLNKLIGDDGETSHLDFLT
                     HDGDNQETSVIKDYERELVEHNIGGALATLNERERYIIQHRIMLNNTMEIERIVNEEQ
                     NKKVESNDEKERSRFDRIRAYKQLVEEGKGDDINQINKIAQKGETVTSAKEAIQAEEA
                     ILRKAKEDIIEFKRAIRSSDREENIAKTLQEIGDRYNIPRELVRQIREQALKEDIAKT
                     LQEIGDKYNITRERARQIEKQALKKVQLALPHLKEDH"
     gene            complement(16590..17204)
                     /locus_tag="PLA58_02245"
     CDS             complement(16590..17204)
                     /locus_tag="PLA58_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOR61645.1"
                     /translation="MTRRSFKKRIGMMLLVMIVLAAGVFYAPRFLAYSTGCARADAVV
                     VLLGPVFHDRDRYARDLMNRGMADTLLIPAYQKIFTVDRGKLRPAFVRKDPDRLNANQ
                     GDEASPYYEDTHLELIEAKRIMRQTGQTTAIFVSSPYHMRRIRIMVGKLFGSSEGHCF
                     LPTPYEKAPVNAWELKASDWKKVGREYVKIAWFLVYSPWAQMTD"
     gene            complement(17232..19856)
                     /gene="mutS"
                     /locus_tag="PLA58_02250"
     CDS             complement(17232..19856)
                     /gene="mutS"
                     /locus_tag="PLA58_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOR61646.1"
                     /translation="MALDATFNLTPAMKQYVEIKEKYPDCILFYRMGDFYEMFFEDAL
                     AAAPLLEITLTSRNKSSKDAVPLCGVPYHAASAYITRLVEKGYKVAVCEQVEDPKSAK
                     GIVRREVVRVITPGLVLDEENLKAGENNYLACLTAGPDALGLAFLDLSTGEFYIGEFP
                     GREALLAAAGGLVFRELLVAGTFPDPVLVKMLEAQMPSVCVNTIDAAGFEPGQADEIL
                     ERELAPSVLEKSGLHKLPAAKIAAAEILRYVKATQQVRAGHISAIREYAPRNYLMLNE
                     AARRNLEIFATLQDGRSAGSLFSLINETLTPMGARRLRWWMAYPLTSPERIRGRLAAV
                     AEIKNSHAAREGLRKILSGVYDLERLAGRVALMAATPRDLVALKTSLAVLPGLRSGLE
                     EFTAPALIALRKDMTDMSATVSLLSRAIVADPPARTQDGGFIAAGFDEELDRLRSISA
                     DGKKWIAALEVKERQATGIHSLKIGYNSVFGYYIEVTKANAALVPDSYVRKQTLVNAE
                     RYINQELKEFEQTVLHAEERIRGRENELFVALRESLLPDIPEIQHNAFRVAEIDATCA
                     LAEAAEKHAYVSPEIDDGDVIDIQDGRHPVVEAALKKEGFVPNDTLLDLRQNRLLVIT
                     GPNMAGKSTYIRQVALIVLLAQMGSFVPASRARIGVVDKIFTRIGASDSLVKGQSTFM
                     VEMMETAQILTEATPRSLVILDEVGRGTSTFDGLSIAWAVAEYIHDFAGQGMRTLFAT
                     HYHQLTDLAASRTGAKNYNIAVKEWGEKIIFLRRIMEGGTSRSYGIEVARIAGVPADV
                     IARAREILRNLEKGEFDEIGMPRIARGQNVRKPANPQLNLFAPSEDVVLEELRRLDVS
                     SMTPLSALNKLNELKSKL"
     gene            complement(19847..20275)
                     /locus_tag="PLA58_02255"
     CDS             complement(19847..20275)
                     /locus_tag="PLA58_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOR61647.1"
                     /translation="MFQGTFMTILLTRWLVITAAILLSSSLLSGIHVASVPTAFLAAA
                     ILGILNTFLRPVLLLLTLPLTVLTLGIFAFVLNALMLLLVAYFVPGLEIDGFFWAFLG
                     ALMISIASWLANRFIYQQPIVHHGKPDVIDLERGDDGKWR"
     gene            complement(20293..21132)
                     /locus_tag="PLA58_02260"
     CDS             complement(20293..21132)
                     /locus_tag="PLA58_02260"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HOR61648.1"
                     /translation="MMTHGRILLAIVLLMLPQQTAGAQKPLWEVGAGGGFLMMPDYRG
                     SDKTRPYLLPFPYAVYRGGIFRLEDKRLTARLFKTDRVTLDASGYGAVPVKSDDNDAR
                     RGMEDLDPTFEFGPALRVKLLDSRENWHRLSLAFPVRAVFSTDFRSVRHEGWVFSPRL
                     NFEKGDVIPGTGLYLGVSTGPMFGDRGYHQYFYNVEPAYATAWRPAYDAGAGYSGSTL
                     TVGLGKNYKSFRFHAFVSADFLQGAVFEDSPLVKTKTSWMSGLSITWVFLKSKKTVMD
                     DTP"
     gene            complement(21145..21645)
                     /locus_tag="PLA58_02265"
     CDS             complement(21145..21645)
                     /locus_tag="PLA58_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOR61649.1"
                     /translation="MDEKIREALREAYVGEAKAVLRLKMFAEKADQEGYPQIARLFRV
                     IAFSEEIHGKRALRVLKEIKTTEENLAESFESEKTVAEIAYDRFVKMAEQAGDKQALL
                     HFSQSRDVEETHAKLYKQAMNHFMEERQTVYHVCKVCGYVADGVLPEECPVCAAKKEQ
                     FTTFDR"
     gene            complement(21672..21830)
                     /locus_tag="PLA58_02270"
     CDS             complement(21672..21830)
                     /locus_tag="PLA58_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOR61650.1"
                     /translation="MKKYVCDVCGYVYDPDQGDEEGGIPPGTPFEKLPADWTCPVCGA
                     DKSEFKPE"
     gene            complement(21852..23063)
                     /locus_tag="PLA58_02275"
     CDS             complement(21852..23063)
                     /locus_tag="PLA58_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOR61651.1"
                     /translation="MTDIYSAIQVSDHVYWVGAIDWSIRDFHGYTTPHGSTYNAYLIL
                     ADKITLIDTVKKPFLEEMLARIASVIDPAKIDYIISNHSEQDHCGCLSEVVGRVRPSK
                     VFASAAGVKNLGEILHNDYVIQAVRDGETLSLGNMELTFMETRMIHWPDSMFTFLAKD
                     GLLFSQDAFGMHLATFERFADELPADMLEYEAATYYANIVLPYSPVVLKALEKVAATG
                     WEIRTIAPDHGPVWRRDLGFILDRYKKWAVQKPANKAVVVYATMWHSTESMAKAIAEA
                     LAQAGVSVKILSLNEAHRSEVIFELLEAGALIVGSSTLNNNMLPQMADVMTYIRGLKP
                     VNLIGAVFGSFGWSGESVKDLETALKGMKVDVVAETVSVRHVPGPEALEKCHELGRTV
                     ASELAKRLSQK"
     gene            complement(23130..23744)
                     /locus_tag="PLA58_02280"
     CDS             complement(23130..23744)
                     /locus_tag="PLA58_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOR61652.1"
                     /translation="MKNKNSGLTKNLILTGVGGQGNVMASRALAGMLVDAGYVVTIGE
                     TFGMSQRGGSVMSHLRISPDAVKSPQIPKGGADVIVALEPLEALRVLTVYGNPGVSLI
                     SNARSIDPMGVITGERDYPSEETIHEAFARLTARCWIFDATAIGLVLGNPVLSNIVMI
                     GALAGTGLLPIDRRMFAKEIRKTLSPEKYALNLKAFDAGCSMIK"
     gene            complement(23809..25767)
                     /locus_tag="PLA58_02285"
     CDS             complement(23809..25767)
                     /locus_tag="PLA58_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOR61653.1"
                     /translation="MTRLLSPAGSRVLFLGNEAIARGALEAGVSVATGYPGTPSSEVI
                     ESLATVARGRNLYVEWSVNEKVALEVAAAASFAGLRSMAVMKQNGVNVASDFLLHLTA
                     SGTRGGLVLVSCEDPGALSSVNEGESRYFAGMLEMPLLEPADFQEAKDMTKWAFELSE
                     AIGNVVMLRSVTRMSHASGNVICGKFPAKTRKAVFRCDGPIMDSQTGPMISTPVVAKH
                     LLRQQKLKTVQALFEKSPFNCYTGPARPDLLLVTASACSLYAAEAVEMLGLQKRVGIL
                     KIGTTWPLPPRLVSGHLRKAGKILIVEEVMAFLEENVKVLAAGDPKLSKGKTFFGKYD
                     GTLPFAGELNPDIVADALAKIFKIRRDALSPSYLEEARKTAAMLPPREQTFCAGCPHR
                     ASFWSIHNAIALDSREGFVCGDIGCYSMAALTPANGYWTMPTLHSMGSGTGLAGGFAK
                     LAPFGFKKPVLTVCGDSTFYHAVIPALINAQHNRADVTFIVLDNQGTAMTGFQPHPGI
                     EVNALKESVPAVDIAAVCRAIGAKVTVCDPFDLAATGAVLTDFLAEKGLLKVLILRQA
                     CALSPEKKGKKTFEMKVDEELCLGDACGCNRLCTRIFRCPALVWDRKKKKAAIDEVVC
                     AGCGVCASICPQKAIVKTPVASAFASGG"
     gene            complement(25852..26985)
                     /locus_tag="PLA58_02290"
     CDS             complement(25852..26985)
                     /locus_tag="PLA58_02290"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOR61654.1"
                     /translation="MRKKRSSQSRLKKDVLEQSLCTGCGACVGLCPYQMVYKDRTVQL
                     FDCDLPDGRCRAFCPRTPSDLPLLRESLFDALDATDEIGPVKEFWIARAKDAGLRARS
                     QHGGTVTALLRLAMSEGWIDAAIVSGRLSEREPEGRLIDDAGALADYARSRFWAAPTV
                     AAFHRAPEGEGRRIGVVATPCQALALAKIRTAKIDGYDKGKTLSLVIGLFCGWTLALE
                     PFQNLLETYQLSAANLTGMDIPAGKNILELHTAEGPVNVPMAEVDACVREACRYCVDS
                     TAEFADLSVGAARYGRNSGEMQNWNQVIVRSETGKKLVSLARSRGILEFRETPQEALH
                     ELKNAAAEKKKKALARIIAKSRSPQNLLYLNPGDTVVKKYLKT"
     gene            complement(26985..27776)
                     /locus_tag="PLA58_02295"
     CDS             complement(26985..27776)
                     /locus_tag="PLA58_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR61655.1"
                     /translation="MNLGLQNKIAIVAAASQGLGRAAARQLALEGATVVICSRREEAI
                     TQAARELREESGSVVLPVKADVTKPQDISHLVSEAEQRFGTVHILVNNAGGPPAGDLL
                     SLTDEDWLRGHDLTLMSVVRLTRAVLPLMVRQEWGRIITINSIVAKQPINELLLSSAV
                     RPGIGGLAKVLANQYAKYNITVNTVCPGHIRTRRQEELAQTRAAARRISVEQYFTETA
                     AAIPAGRLGQPEEIGNAVAFLASEKAAYINGVNLLVDGSAAKGVH"
     gene            complement(27822..29840)
                     /locus_tag="PLA58_02300"
     CDS             complement(27822..29840)
                     /locus_tag="PLA58_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOR61656.1"
                     /translation="MLNPLIRKYNSRKISTQYLLLLIPAILLLFLAFGFVAYTLGKPE
                     ALKNNYELAEVVTKKTNQALMRWLEEQIRTAQTIARDPRIMAVALSPEDQQKRADAQK
                     FLSEMHARYPYYENIPLAVKLAPDRTIAVDVHGEKRLIGNGNFFIDTVGGKTIGKCGP
                     EFSYIRNVFAGKPYFISEVYPSILRGNPIFVVAAPIEHNGEVIGATIVAPRMDYFTEF
                     FVEKSVIGATGYLSMIDQDGMIISHPQKSLILNKKGPTLVKPVTDRIAAGESSFLSDF
                     AGTVKAYYVEKLTTAQFHMEDSWYIVFCRNYDEVLSGIFAFLFKLFISMLLVAVAVAL
                     LVYFLTRRLVGKPMEKLTGAADSVAKGDLRVQIDAGSSRNEVGLLSLSINKMTRSIQE
                     QTIRIREAVDTLNATTASIVSASGQQEAAMKSLEGNVARVGVSSKQISATSQELSGTM
                     ESVRNAAGEASRLANAGQASLSGLQSAMQSLSAGTASISEKLALMRQKAASIDSVIAT
                     ITKISDQTNLLSLNAAIEAEKAGEYGRGFSVVAREIGRLANETAISTLDISDIIAEMQ
                     ASVVDGVREMDHFREDVARGVGSVEDATMQLTQVIARVANLSPEFDKVNEGMRFQSLG
                     SRQISEAMAELNAGAAQLTGSLSQFKAVTQQLNTAARGLSETVSRVIV"
     gene            30039..31346
                     /locus_tag="PLA58_02305"
     CDS             30039..31346
                     /locus_tag="PLA58_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOR61657.1"
                     /translation="MADFNKQYREKLTTFDLAAKTVKDGDTLIHGVTIAEPPGLLAAI
                     ANRARLGDLNHLKVYTFNAQKHFAETLGNPDIADVVDSYSWFVSEGSRNMVQVGLTQF
                     VPAYLHQVPKIVMETMEVDVAVTTVAPMNEEGYFSFGPCNGYLSVMATLCKTLIVEVN
                     ENMPFVYGDALIHISDIEMIVENTVPLLELLPMQPNPLDPIIGKIVAETIPDRAVIQL
                     GIGTLPNAIAPYLDSHRDLGIHTELLCPGMVDLIQKGVITGAHKNVHTGKHIFSLAYG
                     GKETFQYMNGDKSFENYPSSYVMNPAVIAQNHRMTAVNAILQVDLLGQCNAEYLEGRQ
                     YSGTGGQLDFVRGAYDSPDGKAILVLYSTAKNEEVSRIVGQFPAGTMVTTPRNDVHYI
                     ATEYGIVNLKGKSTRARALALISIAHPKFRDELMREAENMSLM"
     gene            31377..31910
                     /locus_tag="PLA58_02310"
     CDS             31377..31910
                     /locus_tag="PLA58_02310"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOR61658.1"
                     /translation="MRKPPPVTAMTSSIASHPALVTGACSLVSVRAIQQGIKAVKRFL
                     GCDVPDVFPCIAEKYANTTTTSHFLVLHDSMLQNKMQAGDNILLISGDLGMLNAIQVL
                     DAMIKSGEAQVGMVIASEINSDRRPDSSYPYPTSGAALPLTLQSAWGGPRLSPGKKGL
                     LLACGSGITAGTAIYYF"
     gene            31963..33522
                     /locus_tag="PLA58_02315"
     CDS             31963..33522
                     /locus_tag="PLA58_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR61659.1"
                     /translation="MTKEELLKEMDQMRKSMKRLQKSEASCKEAADLFRKREQEYREM
                     VELANSIILRMDTSGRIVFVNDFALRFFGFSKEEIVGKNLIGTIVPKMESSGRDLAAF
                     MVSVCRNPGEHTVNENENIKKDGSRVWILWTNRPVLDERGRLAEIICIGNDVTQKKEA
                     EQILQNAHDELEKEVRERTKKLQKLNEDLLFEMAERKISEEALKESENRYRNIVESAF
                     EGIFQTTLEGKCTMVNSALVALLGYSSAEEYIAATARPENLYVDASRREELIRILQTD
                     GYVKGFETEFYRKDGSKLPVSMNVRAVFDDRGNFIYYQGTVIDISAGITLRRVLDETT
                     GALSTAVEIRDPYTAGHQERVTRLAEAIAAEMNFSDERISAVHTAGMLHDIGKIYVPA
                     EFLSRPGKITDNEYNILKQHAQEGYEILKSIEYKYPIAEIVYQHHERINGSGYPRKLS
                     GDRILIEARIISVADVVEAMASPRPYRPALGIDKALDEIRKNRGILYDENVVDACLTL
                     FQDKGFQFGGG"
     gene            complement(33575..34087)
                     /locus_tag="PLA58_02320"
     CDS             complement(33575..34087)
                     /locus_tag="PLA58_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61660.1"
                     /translation="MQVLKFKFGASGDRVDAFGIRFFGDFDGRVGHGGTFSEILTDPD
                     IPPMTTHQRVTLWFMFEKIRPYDKVARMLDHLTEGLKKRGYTIKLSSLDGLVDTTSPE
                     YDSKPERRFPASRRMHGCNAATGFSVTAEKTDAKLKFSSKEISSVKEVAVQLGQEIYG
                     RPLKMVSLQS"
     gene            complement(34148..>34507)
                     /locus_tag="PLA58_02325"
     CDS             complement(34148..>34507)
                     /locus_tag="PLA58_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61661.1"
                     /translation="RYDEKSAKKTFYETVLFESLEFLLVTAGEAVSSGKPEDIDMLNR
                     LTEDRGPMMESLRARHFERDDAASRQTQDYCFDISNLFARLICFLPVLAGRLAYLGRQ
                     QKNRNGDKKTAVIDYDM"
BASE COUNT         7768 a         9984 c         9194 g         7561 t
ORIGIN      
        1 aaaacctgaa acataatcct tgattttcaa accggcttgg gatagaaggc accaacatca
       61 aatacaggga ctccccttgc tcccgaactg aatcgggggc tttagagccg aaaggaggaa
      121 agattgaaga gatacgaagt tatcgctatt gtcaaaacgg atgtgtcgga agaagacctg
      181 accgccatca tggagcggtc ctccaacatc atcaccgaac gcaaaggagt catcgccaaa
      241 gctgagaaat ggggcaaacg gcggctggcc tacgaaatca aaaaacaaaa agacggattt
      301 tatttttaca tcgactatgc cggcgacggt tccatcgtgg ccgaaatgga gagaaatttt
      361 aaaatcgacg accggattct gaaattcatg acggtcgcca aagaaggcgc cgtaacccgt
      421 gagggcatcg atgccgagat cgccgccgcg gaagcaaagc gcacgcagat ccgcatcgaa
      481 acggacaaca cggccgccgc gaaagaggaa aaggcggcag gggatgcaag acctgcgcgg
      541 ccgaccttcc gcaaagcccc gcaccgggaa gacaacacac cgaaaggaga ataatcatgg
      601 ctgcacccaa cggcgaaaga ccatcccgtc cgcctcagag aaagtttttc cacagaaaga
      661 aattctgccg gttctgcacg gactccaacg taaagattga ttataaaaat ccggtgattc
      721 tcaacaactt tgtcacggag cgcggcaaaa tcatgccccg aaggatcacg ggcaactgcg
      781 cttatcacca gagagagctg accgtggcca ttaaaagagc ccgcacgatc gccatcatgc
      841 cttttgtcac cgcggaaggc agataaaccc gttttttaca accgccggcc ggttttattc
      901 tgttgaagga gaaccggccg gtttttattg acccggcaca ccgtttcact gatcaggatc
      961 attgccattt ttttcatcgc gggccgctaa tttgacaata ttgaatttat cgttttttca
     1021 aaattccttt ctccggcttc tgctcaccat cacacttttt ctggcggcct cccttcttcc
     1081 ctttgccgga ctcgttttcc tgttgtttct cccgctggtt ctgtatgtcc tgtgcttcct
     1141 tgccgatccc ggaaaaacac tcatcgcctt tcttgcctcg ctttgcgtcg tgaccatcct
     1201 cctgtgggcc ctgaatgcgt ccgcgtctgc gccggccctg gcggcggccg ggttcgccgg
     1261 aatgctgatg ttttggaccg tccaaaagcg cttctccgtc gaaggggttg tccttctgcc
     1321 gtcccttctt ttggtgggcg caatcgcttt atattttatt tacggcggca tgcaactttc
     1381 cataactccc tggcaactgg ttgaaaagcg cattcaggag gcggtggaac tcaacatccg
     1441 cctctacagt caatttcctc taaatgctga agaaattcaa gaaattacaa acagcaagcc
     1501 gcagatgatt tcgctgttca cgatgatttt ccccgccctt tgcgtcatag cggccgtatt
     1561 caccatctgg atcaatctgc ttgccgcggg caggctgctg aaaggccgag gcattttgcc
     1621 ggccccgctt tcggccctat ccgaatggag cgcgcccgcc tggctggtct ggatttttat
     1681 tgccgcggga ggcatgagcg ccgtcccaat ggcccccgtc cgcttttcgg gcgtcaatct
     1741 ttttcttgtc gcgtcattta tttattttct gcaaggcctt gctattgtta gtttcttctt
     1801 tcaacatcga aacatctccc cttttttccg ctttcttgct tattttttaa ttgcgattca
     1861 acaaatgctt atgatagcaa tagctgccgt ggggctcttc aatctctggg ttgattttcg
     1921 caaatatctc cgcaaagacc ggacaacaga ctgacgatgc acgacaggag gtttgaccat
     1981 gaaggtgatt ttaattcaaa acgtgccttc cctgggcaaa gccggagact tggtcaaagt
     2041 caacgacggc cacgcccgca atctgctgat tccaaagggt ctggccgttg aggcggacca
     2101 taaaaacatc aaggccttcg aactggccaa aaaaaacatt ctgcaaaaga cacagaaaga
     2161 aaaggagggc gcacgggatc tggcctcccg cttaagccag gtgacgctga cgctatcgcg
     2221 caaagtagga gatcaacaca agatcttcgg ctcggtcacc tccaaagaca tcgaagccgc
     2281 tcttcgggaa aagggatttt ccgtggaccg gaaaatgatt gtgcacgatg agccgatcaa
     2341 atccctggga gagttcaagg ttcgcatcaa actgcacccg ggcgttgaaa cggaaatcaa
     2401 gatcattgtt gccggcgaag aatcatgaaa gacatcgatc cttccttata caaactgccg
     2461 cctcagaaca tagaggccga acagtccatt ctgggcagca ttctgctgga gaacagcgcc
     2521 atcaacgcgg cgcaggaaat cctgaacaag gatgattttt acagcgaagc gcaccggaag
     2581 attttttccg tcatcgagca gctggccgac aaaaatgagc cggttgacct gatcacgctc
     2641 ggcaacgccc tgaaggacaa gaccctgctg gattcggtcg gcggcatcgc ctacctggca
     2701 tcgctggtgg acaacgtccc ctcggccgcc aacgtggcca attacgcaaa gatcgtgaag
     2761 gaaaaggcga ttctgcgcgg cctgatcgga tccgccaccg aaatcatcaa cagttgctat
     2821 gagacggact ccgacgtcga tcaggtgctg gataaagccg aacaccgcat ttttgaaatt
     2881 tccgaaaaca aggtgcggac gtctttttat ccgatcaaag atatcgtccg ggagagcttc
     2941 cgggccatcg aggatttgtt cacccgcaag gaactgatca ccggcgtcgc caccggattc
     3001 gataaaatcg acgacatgac ttccggcctg caggcttccg atctgatcat tgtcgccgga
     3061 cgccccagca tgggcaagac cgccttcgcg ctcaatatcg cccaacacgc cgcaatggaa
     3121 acccagacgc cggtggccat tttttcgctg gaaatgtcca aggagcagct cgctttccgc
     3181 ctgctcgcct ctgaagccag ggttgattcc cagaggctcc gcaaaggatt cctgggcgaa
     3241 accgactggc cgaagctgac ggcggcggca ggcaggctct ccgaagcgcc gttatacatt
     3301 gacgacacac ccgccatcac cgttctggaa atgaaagcga agtcccgccg cctgaaagcg
     3361 gaccgcgatc tgggactgat cgtcgtggac tatatccagt tgatgcgcgc ccagggcaac
     3421 caaaattccc gcgagcagga aatctccgaa atcagccgct cgctcaaagc gttggccaaa
     3481 gagctcaagg tgcccgtgat cgcgctttcc cagctcaacc gcaaggtgga agaccgtccc
     3541 aaccggcggc cgcaaatggc ggatttgcgg gaatccggcg ccattgaaca ggacgcggac
     3601 gtgatcgcct ttatctaccg ggatgaggtg tacaacaaat ccgatgacaa cccggacaaa
     3661 ggcgtcgccg aaatcatcat cggcaaacag cgcaacggcc ctaccggcct ggtgaagctt
     3721 gcgttcctgg accggttcac cacttttgaa aatctggcgc gtccggatgg accgtcgtaa
     3781 aagacgaagc gcgtccgaac ggcaacatgc atggatgggg tgcggatcac atgaggccca
     3841 aagaagtcat cgtcaatgat cggatgcaaa agggttaccg atacgttctg accaaaccgg
     3901 ccggaagaga ttttgacccg gacttcaagc cggaactgac gcccaaacaa atgctggaaa
     3961 tcggcgtctt cggcggaaaa tacatgacgg actgccgcag ggaatttccc gcctcctggt
     4021 ttgccaaagc cagactctct cccgaacgcc atgatccgaa gctcaactgc ttcggcgtca
     4081 acgcctccaa acccctctcc tactgggtgg ccaaaggctg gattcatccc gacgatcccc
     4141 ggggctggtt tcagtggtac tgccgctatt acttgggccg ccgccgcccc gatgatgaaa
     4201 gacaaatcaa acgctggagc gccatgcagc ggcacatctc ccaactcacc aaaaactgcc
     4261 ccgcgggcga tctttcctgc cgccgcaaac agcgccaggc cctgctccac tgggcgtacg
     4321 acagcaggaa aatgtaagat atctaacggc gaggcaacgg cgcttctttc gtgcaaagga
     4381 tgcgccgttt ttcgggagcg tcattaccgt tcagaatttc ggcgatttct tctgtttctg
     4441 tccccacgga cacatcattc tgacggaagc tgctcccttc aaaaagatca ggacaaacgt
     4501 aatactgatc tgtcatgata agcctgtcga accatggggc gttatatcat acgggcttct
     4561 cgcaggcgca tacggcagaa aaagggacaa ccatttttct ttcagacgcg gcggtcaggc
     4621 ccgcctgact tccaatgtaa atggacttcc cctgcagccg tgccggcttg aagatttaca
     4681 gggcctatta tcaaggaatc gctatcccgg ttatttcgtt cgctcactat cgcatgtgcc
     4741 ccataatctt ctggctaagt tcattcttga caatagtaaa caatgtgttt actatgaaac
     4801 tatgattaaa tcgtttcgat gccgtgaaac ggagaaaata ttccgcagag aacgctccag
     4861 caagtttccg ggcgatattc agcagagggc ttttatgaag ctcaacgccc tggatgcagc
     4921 agttaaaatt gaagacctcc gcctgccgcc gtctaaccga ttggaagccc tgcgggggaa
     4981 aaggcatgga cagtggagca tccgaatcaa cgatcaatgg cggatttgtt ttgtctggca
     5041 aaaagatcat gccgaaaaag ttgaaattgt cgattatcat taaggagcaa ggttatgttg
     5101 aaaaaacgga ttaaaccggt ccatccggga gattatttaa aagagctgct ggacgagctg
     5161 gaaatatccc agtaccggct ggcccatgat ctgggagttg ggaccatgag gataaaccat
     5221 gtggtcaacg gcaagcgtcc ggttaatgcg gaactggcat tgcgattggg caggtatttc
     5281 agccaggaac cacgctactg gatgaatctg caaagccggt atgacatgga tatggcagaa
     5341 gatgctcttt ccgatcaggt atcccatgaa gttcagccgc tgagaatggc gggataatgc
     5401 tgcctacgtt agcgtaaacc atgttcccgg gtatgtaaaa aaagggcgct ccttttgggg
     5461 gagcgccctt ttggtttaaa ggggatggaa aattatccgc ccagtttctc gctggcgatt
     5521 ctcatggagt agaaggagcg gtacacgaaa atcagggcaa tcagcaggaa gacaaccgcc
     5581 agaccaatct tcaggttctg gttggtcatg gtcaccgcga tttccgtagc cagaagaccg
     5641 aacagggtcg tgaatttgat gaccgggttc aaagaaaccg aagaggtgtc tttgaagggg
     5701 tcgccgacgg tgtcgccgac cacgctggct tcatgcagcg gggtgttttt catcttgaga
     5761 tccacttcaa caatcttctt gccgttgtcc cagcagccgc cggcgttggc catgaagatc
     5821 gcctggaaca gaccgaagaa ggcgatgccg atcaggtagc cgatgaagaa gtaggggttg
     5881 aagaacgaaa gggccagaca catgaagaag atcacgatga agatgttgat catccccttc
     5941 tgagcgtact gggtgcaaat ctgaacgact ttcttggaat cctgaatcga ggcttccgcc
     6001 ttgtccagat tgatgttctt cttgatgtac acaacggcct gataagcgcc ggtggtcacg
     6061 gcctgcgtgg aagcgccggt gaaccagtaa atgaccgcgc ctcccatgat caggcccaga
     6121 ataatctcag gctgaaccag ggacagcttg gaaatgacct gtccgaagag accttcgagc
     6181 aggatgatga tgccgaacac catggtggtg gcgccgacaa cggcggttcc gatcaacacg
     6241 ggtttggccg tcgctttgaa ggtattgccc gcgccgtcgc cggattccag ataatgtttg
     6301 gccagctcaa agtcgggagc gatgccgtac gccgccttga cgacgtcctt ggcatccttg
     6361 cgggattcga tcatggacag ttcataaatg gactgcgcat tgtcggatac cggaccgaag
     6421 ctgtccaccg cgatggtgac ggggcccatg cccagaaaac cgaaggccac cagaccgaac
     6481 gcaaagaccg gcgccgcaaa ggtgaatgcc gggggcatca gcgccataac ggacgcattc
     6541 ttggaaacca tgtaagagac aaacatcaga agcacaacca ccattcccag ccagaaaccg
     6601 gagaagttcc ccgcgacgaa accggacagg atgttcagag aaggcccgcc ctgtttggaa
     6661 gcgttgacga cttcttcgca gtgacgggaa gaggtgctgg tgaagatttt ggtgaattcg
     6721 ggaatcaacg cgccggcaat ggttccgcag ctgatgatca ccgacagagc ccaccacagg
     6781 tctgcgtatt tgggatcaag gtcatttgcg ttcagcagga aataacttgc cgcaaaggta
     6841 acgccgatgg aaacgatcga ggtaatccag accaggttgg tcagcggatg ctcgaaattg
     6901 aattctttct ttccggcaaa agcagcggtg ctgatgccgt tgttgacaaa gtaggaaacc
     6961 agagacgtca ggatcatgag gattctcatg gcgaaaatcc agatgatcag cttgccgccc
     7021 atttcggggt tgccggccag agccagtgcc aggaaggtga tcagcgcgac gccggtaacg
     7081 ccgtaagttt caaaaccatc cgccgtgggg ccgacgctgt cacccgcgtt gtcgcccgta
     7141 cagtcggcga tgacgccggg gtttttcggg tcgtcttcgg gaagatggaa gacgatcttc
     7201 atgaggtcgg aaccgatgtc ggcgatcttc gtgaagatac cgccgcagat acgcagcgcg
     7261 gaagcgccca gcgattcacc gatcgcgaaa ccgatgaaac aggggccgac cagttctttc
     7321 gggatataac ccaggatcat gatcatgaag aacagttcga tgctcaccag cagaaggccg
     7381 acgctcatgc cggaacgcag gcagatattg acgatgttga ggggattgcc ggaaagcgac
     7441 gagaaagcag cgcgggagtt ggcaaccgta ttaatgcgga taccgaacca ggccaccgag
     7501 taggagccca gaatacccag aatcgaacag accaggatga tcacgatttc gccggcaggc
     7561 attccggaca gaccgccaaa atagtaaatc atacaaatgg caatcaggat ccataaaccg
     7621 ataaggaact tgccctgctg gaacaggtag gttttgcagg tttcccagat cgtctgggaa
     7681 acatcgagca tcgcctgatg ggccggcagg ttcttggtct gtacatactg catcacaccg
     7741 aaaatcaacc cgataatgca gatgaccaag ccgacgttca gaatcatcat gccggaaagg
     7801 gcgccgccca taaaactgac ctgacttaag tccggcagtt tgatgtccgc ttcactggcc
     7861 agggccgcgc cagcggaaag cagaaccagc aaaccgctca gtaaaaatgt ccaaatactt
     7921 cttttgtttc taaacattca ctattcctcc ttttttagaa aatctatatt ttttgtctgg
     7981 tacttgctta tcgctttctg cagaccgttc aaaacaattt cagcgctcgc atcggccgcg
     8041 cgatgaatca cttccggcag cataacctgc tcctccttcc ggaagggctc caagacataa
     8101 ccctcaattc gggatttgtc aactggtttg ccaataccca gtctcacccg cataaaaccg
     8161 gaagttccca agttagactc gatagatgcc agtcctttat gaccggcggt tcccccaccc
     8221 gccttcatcc gcacagcccc gtagggcagg tcgagatcat cgtgaataac gatgagattg
     8281 ctgatttccg ttttaaagaa cgactgcaac tgctttaccg cagtaccact caaattcatg
     8341 aatgtctggg gcttggccag aagaacattt ttaccttcaa cggtcccttt accccacaac
     8401 gcattgaaac tcttctgttt taaatcaact ttccacctgg cagccagaag gtcggccacc
     8461 ataaacccga tattgtggcg ggtgaattcg tagcgtttcc ccggatttcc cagtccgaca
     8521 accagataaa ccacttgtcc cctctctttt tgaaaacgga aaagatattt tccaaagctg
     8581 aagagacgca tgatctcccg tataattcct ctgttccctt atttcttttc cttttccttc
     8641 ttttcttctt tcgccggttc cgcggcggcg gcagcgcctt ccgcagcttc agcaccttcc
     8701 gcggcaacgg cttcggcgga ttcaaccttg gccacccgga cggcggcgac ggacacgaca
     8761 gcggcgtccg gcggatccag atgggtcaat ccctcaggaa tgacgatatc tttcactttc
     8821 agggaatggc cgatttcaag agctgaaata tcgacatcga tgtggtcggg cagatcggtc
     8881 ggcagacaca acaccctgac gtctcttttg atatgctgga gttccccgcc gttttccacg
     8941 ccgacggcct tgccggtaaa gttcagttcg acgtcaaagg ccagcttctt attcatatcc
     9001 acttcataaa aatccgcgtg atagaatttc ccgctcaggg gctggatctg caattccttg
     9061 atcagggaaa gcttctcgat tttcttgccg ccgtcatcaa tcagcagctt gataaaggcg
     9121 tggtctttct tttgcttgtg cagcctgaac aggtcgctgc ttttcacggc cagcgggatg
     9181 ttccccgtgg ctttgccgta aaaaatggcc ggcacaagtc cctgctgacg cagccttcgg
     9241 gccgggccct tcttgtgttc tgtacggacc tgggccgtta aatcggttat ttccatttac
     9301 cccttccctc ctaaataaat aatgaactca ctgaatcgtt gtaataaatg cggcgcaccg
     9361 cttcggacaa caggccggac accgacaaaa cttttattct ttcacattgg gccgcctttt
     9421 cacaaagcgg aatcgtatcc gtcacgacaa attccctgat ggcggatttg ctgatgcgat
     9481 cgatcgccgg accggacaac accggatgcg tgcagcagac ggatacttcc agagcgcccg
     9541 cgtccgccag cgcctgggcc gcctgcacga cggttcccgc cgtatccacc atatcgtcca
     9601 gaataatgac cctctttccc ttgatgttgc cgataatatt catgacctgg gcttcattgg
     9661 ggccttccct gcgtttgtcg ataatcgcca gagaggcgcc cagccttttg ccgaacgccc
     9721 gggcccgttc gacgccgccg gtatccgggg aaaccaccac cgtatcctcc ctgtaatttc
     9781 ttttcatgta gtcaatcaac accggcgtgg caaagaggtt gtccacggga atgttgaaga
     9841 agccctgaat ctggccggcg tgcaggtcca tggacaaaag gcggttggcg ccggccgtcg
     9901 taatcaaatc cgcgaccagt ttggccgaaa tgggcgcgcg gggcgccact ttccggtcct
     9961 gcctcgcata tccgtagtac ggcacgacgg cggtgatgcg gtcggcagac gcccttttca
    10021 tggcatcgat cataatgagc agttccatca gggtgacatt caccggcgtg caggtggact
    10081 ggatgatgaa aacatccatc ccccgaacat tttcatcaat ctccacgcgg gtttcaccgt
    10141 cgctgaaggt ggttacattg gcgttgccca gcggaacccc cagcttttca cagattttcc
    10201 gggccagttc ccgattggca tttccggtaa atacccttat tctttcaagc atttaaatat
    10261 ttctcccaaa gcttttctgg ctggggcggg aggattcgaa cctccgaatg caggaaccaa
    10321 aatcctgtgt cttaccgctt gacgacgccc caacgtcttc gatttataat tcaaccgggc
    10381 agttcttcgt caggggagaa ttcgtattta cccgagagtg attgggcaaa gaaaacgcga
    10441 cagtctccgg aaactttttt ttcaatagca ctccgcgctt ctctggccga ctcttcatcg
    10501 gcaaaaaggc caaatagcgt gggaccgctg cccgacatca atgcacccag ggcgccttcg
    10561 ttgatcagca actgtttaaa atccgccaat tcgggatgca tgttcatcga tacagcttct
    10621 aaatcattat gcaattcccg aattacatca cccagtgcgt aaaatcgcgg aatgctataa
    10681 ttattttctt tttttgtcaa cgtgaaatta aggttttcgt aaaccgtttt tgtggaaagg
    10741 ggaaaggggg gattgattaa aaccatactg agttttggcg tgttttccag cgcctggagc
    10801 ctgtcgccga tgccggatgc aaacgccgtt tggccaaaaa taaaaaaaga cacatccgcg
    10861 ccgagtttta cccccagttt catcagctcg tctttggaaa gccccagtga acacaggtca
    10921 ttgagcgcca gcagggtggc cgcggcatcc gaactgcctc cacccagacc ggccgccgaa
    10981 ggaatctgct tggagagcgt gatgtcaacg ccgcccggat agccggtatg ttcaaaaagc
    11041 gcccgggccg cccggtaaac caggtttttt tcatccgtgg gaaggtcggt tccggggcag
    11101 gaaagcgcaa tcccctccgg gcggagggaa cagatcagtt catcgaaaat ggaaatctgc
    11161 tgcatgacgg aagccagatc gtggtaacca tccggcctct ttcgcaaaat tcgcagaaaa
    11221 aggttgattt ttgcaggcgc cagccttcgc atttcagcct ttttctttga catagcgcat
    11281 tttcagctcg tagagcactc cctcgatcag attggtttcg tttttgtcga ggtttccggc
    11341 ggttttcttc tggagcatat ccagaatatc gatggtctgt ttggcagccg gaagattctt
    11401 ttcggctttt cccccttcga cctccggaaa atcgccgaaa tgaaacagag cggacgtgga
    11461 aagcgaaata acaaaattcg taaaattaac aggtggatac tcatccggtt ctgccggagt
    11521 ttttccggcc tgtcccttcg gttcctccgc ttttacggat gccggctcct cttttgcatt
    11581 agcatccggt tcttccgaaa aacgcctgtc cctgatgaca aatccttcgc ctttggtttc
    11641 ttccgccata ataccccctt attttgcagg gaacggccgc gaccgttccc tactctttat
    11701 ccttcagtct tcagccttca gccttcggtc ttcagccttt cttactgaac gtgatcgccc
    11761 ctttcgaaac gccgatgacg atgtggtcgc cttcggcaaa tttgcctgcc agaatgtcca
    11821 tcgccagcgg gtccaaaaca agcttctgca gcgatctttt gagcggccgg gccccgtaaa
    11881 ccgggctgta gccctcttcg gctatatatt ttttcgccgc gtctgtcaat tcgatggaga
    11941 gcttgcgctc cttgagccgc ctgtcgatca gttcgagctg aatgccgacg atccggtaaa
    12001 tgtcgtcaag cgtcaagccg tggaacatga tgatgtcgtc gatgcggttc aaaaactccg
    12061 gcttgaaggt cgcgcgcagc gcttccatcg accggttttt cttttcctcc tcggaaagcg
    12121 acggatcctg aatccacccg cttccgacgt tggaggtcat gatcacgacg gtgttcttga
    12181 aatccaccgt gcggccgtgg ccgtcggtga gacggccgtc gtccagaatc tgcagcagga
    12241 tgttgaagac atccggatgc gccttttcga tttcgtcgaa cagcaggacg ctgtaaggct
    12301 tgcgccggac ggcctccgtg aggtagccgc cttcgtcata gcccacgtag ccgggcggag
    12361 cgccgatgag gcgcgccacc gcgtgctttt ccatgtactc ggacatgtcg atgcggatca
    12421 tggcctgctc gttgtcgaac atgaactccg ccagcgcccg cgccagctcc gtcttgccca
    12481 cgccggtcgg ccccaggaag atgaacgagc cgatgggacg gttcgggtcc tgcaggccgg
    12541 agcgcgcgcg ccgaagcgcc gccgatacgg cctcgattcc gtcgtcctga ccgatcacgc
    12601 gcattcgaag ccgctcgtcc atatgaatga gcttctgcac gtcgctttcc atcatgcgtg
    12661 aaagcggaat gcccgtccag ttggaaacca cttcggccac gtcttcggca tcgacttcct
    12721 ctttgagcat ctggttgttt ttctgaacgt ccgaaagctt cgcctgctcc gtctccagct
    12781 ccctggcaag ctccaccatt ttgccgtagc ggatttcggc cgcgcgcgcc aggttgcctt
    12841 cgcgctgcgc gcccgcttcc tccgtcttca gcgcctccat ccggctcttg atctgctgga
    12901 tctttttgat gatgtccttt tccgacgacc agtgcaactt catccggtcc atctcgcttt
    12961 tgagttcagc cagctctttt tcgatttttt cgcgccgctc cgtcgccgcc ttgtccgtat
    13021 ccttcttgag cgactgtttt tcgatttcca attgaataat tctgcggtcg atcgcatcga
    13081 tctcggacgg catcgaatcc agctcgatcc ggaggcgcga cgcggcctcg tcgatcaggt
    13141 ccaccgcctt gtccggcagg aaccggtcgg tgatgtagcg gtgggagagc gtcgcggcgg
    13201 caacgatcgc cgagtccttg atccgcacac cgtggtgcac ttcatagcgc tccttgaggc
    13261 cgcgcagaat ggcgatggtg tcctccaccg tcggctcctt gaccagcacc ggctggaagc
    13321 gccgctcgag cgccgcgtcc ttttcaatgt acttgcggta ttcgttgagc gtcgttgcgc
    13381 ccacgcagcg cagctcgccc cgcgccagcg ccggcttgag catgttggag gcgtccatcg
    13441 acccttccga ggcgcccgct ccgacgacgg tgtgaatttc gtcgataaag agaatgatct
    13501 cgccttcggc gctcgtgacc tccttcaaaa ccgccttgag cctttcttcg aattcaccgc
    13561 ggtatttcgc gcctgcgatg agcgcgccga cgtccagacc gatcacgcgc ttgtctttca
    13621 ggttttccgg cacgtcgccg ttgacgatgc gctgggcaag gccttccacg atggcggttt
    13681 tgccgacgcc cggctcgccg atcagcaccg ggttgttctt cgtgcgcctc gacagcacct
    13741 gcatgatgcg gcggatttca tcatcgcggc cgatcaccgg atcaaatttt cctgtacggg
    13801 ccagttcgtt gaaatccttg gcatagcgct cgagggcctg atacttgcct tccggattgg
    13861 ggtccgtcac gcgctgattg ccgcggatat cgaccagcgc cttgaaaata ttgtccctcg
    13921 tgacgccgtt ttgacgcagc gttttacccg cctgcccttc cttctcctcg gacatcgcga
    13981 ccagcacatg ctcggcgctc acgtactcgt ccctgagctg tgccgcttcc gtcatggcct
    14041 ggtcgaacag ccggttgaga cggccgccca gatacacctg gcctgcgccc gagcctccga
    14101 cacggggctg ctttttcaaa taagcgtcca gttcccgttc tattttttgc ggggatgccc
    14161 ccagcttcgt cagaacggcg gaaacgatgg agtctggctc ctgtaaaaaa cacaccagca
    14221 gatgctccgg ctcaatggcc tggtgcccct gcgaggcggc catcgtctgg gcgtcctgaa
    14281 gcgcttcctg cactttcaat gtaaatttat caaatctcat ggactccctc cttctactcc
    14341 acgggttttt caatcgtcac aaaaagcttc ccgtttttaa aaatactgac gtttcccgag
    14401 gactgggaaa tcaccatcgc caccgcattg gtcacgttgg tgatgcccgc cgcggcgata
    14461 tggcggctgc ccagccccga cggaaaatct ttgctgtcca gcgccgcgct caagtgccgg
    14521 cctgccgtaa caatcgctcc gttggccttg atgataaacg cgccgtcaat ggcggaaagc
    14581 tccttgatgg tttcctccag ctccggattc agaatattgc gctgctcctc ggagtatcct
    14641 ttaaacgggt tgatgatcat ctgccgggac aactgcatga ctttctcatc gtcccccagc
    14701 acaaagatcg ttccgacctt ccgggtttcc cgcccctgag cggcaagctc acaggcgatg
    14761 ttcaacgccg cgttgaaaac ctccggctgg acggaatcca cgacatcgct gatgtcgtcg
    14821 gatgtcagaa tttcaaattc gcgccccacg tcgatgaaaa agatgctgtc cgcatatccg
    14881 aatttcggca cgcccgacag gcagacgatt ctgtcgcctt ttttgaacag tccggaaaca
    14941 atgcctttgg cgatcgcgat cttgattttt ccgacgcggg tcagattcac gtcgggaagt
    15001 tccagtttct tcgccgtcgg cagcagcttc tccggcaatt cttcggattc tctcatgacg
    15061 aaaacgaacc gaaccgattt tctctcgtct tcggaaagct cggccagttc accgcgaaca
    15121 tctacgcaca ggagaacaca gccggctttg acctctttcg ccagtttaat ggctgattcg
    15181 accatcgtct tgttcacgga tttcatatcc ataatccctg gttttttgaa ggtagaataa
    15241 tcattcggcg cccgcctgtc aagacgggga cgaccgcccg gggaaatatt tactggaaaa
    15301 tatttgcttg accgccaatg gactcttcgg tattttaata aaccatgttt ttttatcgag
    15361 gaggccagca cgttgaagtt acccatcttc accggcactc tggacttata catcgctgag
    15421 atcaatcgct tccccatttt aacccccgag gaagagttca agctggccat gcgcctgaag
    15481 aaagacaacg atatggaagc ggcggaaaaa ctcatcgttt ccaacctgcg gtttgtcgtg
    15541 aagatcgccc acgaataccg cagctacggc ttcaaactgg ccgatctcat tcaggaaggc
    15601 aacatcggac tgattcatgc cgtcaaaaaa ttcgacccgg aaaaaggcta ccgcctgatt
    15661 tcctatgcgg tctggtggat tcgcgcctac attcagaact acctgattaa atcctggagc
    15721 atcgtcaaga ttggaaccac gcaggcgcag aggaaacttt ttttcaagtt gaaccaggcc
    15781 aggaaacagc tggaaaccat ctcgaagaag aatcccgaat tcagcgagat cgccgattca
    15841 ctgggcgtca aggggtccga ggtcgcggaa atggatctgc ggatgggcgc gcgcgacctg
    15901 tcgctcaata agcttatcgg tgatgacggc gaaacgtccc atctcgattt cctcacccat
    15961 gacggcgaca accaggaaac atccgtgatc aaggactatg aacgagaatt ggttgaacac
    16021 aatatcggcg gcgccttggc tacactcaat gaacgggaac gctacatcat ccagcaccgt
    16081 ataatgttga ataatacgat ggaaatagaa cgtattgtta acgaagagca aaataaaaaa
    16141 gtagaaagca atgatgaaaa agaaagaagt agattcgata ggattagagc atataaacaa
    16201 ctcgttgaag aagggaaagg cgatgatatt aaccaaatta ataaaattgc gcagaaaggc
    16261 gagacagtta catccgctaa agaggctata caagcagaag aagcaatcct aaggaaggca
    16321 aaagaagaca ttatcgaatt taaaagagct ataaggagtt ctgatagaga agaaaatatt
    16381 gcaaaaaccc ttcaggaaat cggcgacaga tacaacatcc cccgtgaact cgtccgtcag
    16441 atcagggaac aggcgcttaa agaagatatt gcaaaaaccc ttcaggaaat cggcgacaaa
    16501 tacaacatca cccgtgaacg cgcccgccag attgagaagc aggccctcaa aaaagtgcag
    16561 ctggccctgc cgcacctaaa agaggatcat taatcggtca tttgggccca cggggaataa
    16621 acgagaaacc acgcgatttt cacatactct ctgcccactt ttttccagtc ggacgccttc
    16681 agttcccagg cgtttaccgg ggccttttcg tagggcgtcg gcaaaaaaca atggccttcg
    16741 gatgaaccga acagtttgcc gaccatgatg cggatgcgcc gcatatgata cggggagctg
    16801 acgaagatcg ccgtcgtctg accggtttgc cgcatgattc ttttcgcctc gatcagctcc
    16861 aggtgcgtgt cctcataata cggcgaagcc tcatcgccct gattcgcatt gaggcgatcg
    16921 gggtccttcc ggacgaaagc gggccggagc ttaccccggt caacggtgaa gatcttttga
    16981 taggcgggaa tcaggagcgt gtccgccatc ccccggttca tcaaatccct ggcgtagcga
    17041 tcccggtcgt gaaaaacggg gcccagcaaa accacgacgg catccgcccg ggcgcagccg
    17101 gtcgaatacg ccagaaaccg gggcgcataa aacacccccg ccgcaaggac gatcatcacc
    17161 agcagcatca ttccgatgcg ctttttaaag gaccgccggg tcatgggccg atgccgttcc
    17221 tgtttccttt ttcataattt gcttttcagt tcgttgagtt tattgagcgc ggaaagcggc
    17281 gtcatggaag aaacatccag ccggcgaagc tcttccagaa cgacgtcttc gctcggggca
    17341 aacagattca actgcgggtt ggccggcttt cggacatttt ggccgcgggc gatccgcggc
    17401 atgccgattt cgtcgaattc gcccttctcc agattgcgca gaatttccct ggcccgggca
    17461 atgacgtccg ccggaacgcc ggcgatcctc gcgacttcga tgccgtagct gcggctcgtt
    17521 cccccctcca tgatcctgcg cagaaaaatg attttctccc cccattcctt gacggcaatg
    17581 ttgtaatttt tcgcgcctgt cctggacgcg gccagatcgg tcaactggtg gtagtgtgtg
    17641 gcaaacagcg tgcgcatgcc ctgcccggcg aaatcgtgaa tatactcggc cacggcccag
    17701 gcgatgctta acccgtcaaa cgtcgaagtt ccccgaccca cctcgtccag aatgaccagg
    17761 ctgcggggcg tcgcctccgt gagaatctgc gccgtctcca tcatttccac cataaacgtg
    17821 ctctggcctt tgaccaggct gtccgacgcg ccgatccgcg tgaagatttt atcgaccacg
    17881 ccgatgcggg cgcgggaagc gggaacaaaa ctgcccattt gcgcgagaag cacgatcagg
    17941 gccacctgtc ggatatatgt ggacttcccg gccatgttgg gcccggtaat gacgagcagg
    18001 cggttttgcc gcaaatccag cagcgtatcg ttgggcacaa acccttcctt tttgagggcc
    18061 gcttcgacca ccggatggcg gccgtcctga atgtcgatga cgtctccgtc atcgatttcc
    18121 ggcgagacgt aggcatgctt ttcggccgct tccgccagcg cgcaggtcgc atcgatctcg
    18181 gccacgcgaa aagcgttgtg ctgaatttca ggaatatccg gcagcaggct ttcgcgaagc
    18241 gcgacaaaca gttcattttc gcgcccgcgt attctttctt ccgcgtgcag caccgtctgt
    18301 tcgaattcct tcaattcctg attgatgtag cgctccgcgt tgaccagcgt ctgcttgcgc
    18361 acgtaggagt ccggcaccag cgcggcgttg gctttggtca cttcgatata gtagccgaac
    18421 acgctgttgt agccgatttt gagggaatgg atgcccgtgg cctgccgctc tttgacctcc
    18481 agcgcggcaa tccatttttt gccgtcggcg ctgatggagc gcaggcgatc cagctcttcg
    18541 tcaaagcccg cggcgataaa tcccccgtcc tgcgtgcggg cgggaggatc ggcgacaatc
    18601 gcgcgggaaa gcagcgaaac cgtcgcggac atgtccgtca tgtctttgcg caaggcgatc
    18661 agagcgggcg cggtaaattc ttccaacccg gatcgcaggc cgggtaaaac ggcaagagac
    18721 gttttcagcg ccacgaggtc gcgcggcgtc gccgccatga gcgcaacgcg tccggccagc
    18781 ctttccaaat catagacgcc ggatagaatc ttgcgcaggc cttcccgcgc cgcgtggctg
    18841 tttttgattt ccgccacggc cgccagccgt ccgcggattc tttcgggcga cgtgagcgga
    18901 taggccatcc accagcgcag ccgtctggcc cccatcggcg tcagggtttc attgatcagt
    18961 gaaaaaagag agccggcgct tctgccgtcc tgaagggttg cgaagatttc caggtttcgg
    19021 cgcgcggcct cgttgagcat cagataattc cggggcgcat attcccgaat ggcggaaata
    19081 tggccggcgc gaacctgctg cgtggccttc acatagcgca gaatctcggc ggcggcgatt
    19141 ttcgccgcgg gaagcttgtg aaggcctgat ttttccagca ccgaaggcgc gagctccctc
    19201 tccagaattt catccgcctg cccgggctca aagccggccg cgtcaatcgt attgacgcaa
    19261 acggacggca tctgggcctc cagcattttg accagcaccg gatcgggaaa ggtcccggcg
    19321 accagcagtt cccgaaagac caatccgccc gcggcggcga gaagcgcttc gcgcccgggg
    19381 aactcgccga tgtaaaattc accggtggaa agatccagaa acgccaggcc cagcgcatcg
    19441 gggccggcgg tcaggcaggc cagataattg ttttctcccg ctttcaggtt ctcctcgtcc
    19501 agaaccagcc ccggcgtgat cacgcggaca acctcgcgcc ggacgatgcc tttggcgctt
    19561 ttcgggtcct ctacctgttc gcagacggct actttgtagc ccttttccac gagccttgtg
    19621 atgtaggcgg aagcggcgtg atagggaacc ccgcacagcg gcacggcgtc tttactggac
    19681 ttattgcggg aggtgagcgt gatttccaga agcggcgcgg ccgcaagcgc gtcttcgaaa
    19741 aacatctcgt agaagtcgcc catgcggtaa aacaaaatgc agtccggata tttttccttg
    19801 atttcgacgt actgcttcat cgccggggta agattgaacg ttgcgtctag cgccatttcc
    19861 cgtcatcccc ccgctccaga tcgatcacgt cgggtttccc gtgatggacg atgggttgct
    19921 gataaatgaa acggttggcc agccagctgg cgatgctgat catcagggcg ccgagaaacg
    19981 cccagaaaaa accgtcaatc tccagacccg gcacgaaata ggccaccaga agcagcatga
    20041 gggcattgag cacaaaggca aaaatcccca gcgtcagcac cgtcagcggc agggtcaaaa
    20101 gaagcaggac gggacgaagg aaggtgttca gaattcccag aatcgcggca gctagaaagg
    20161 ccgtcggtac ggaagccaca tgaatgccgg aaaggaggct ggacgagagg agaatggccg
    20221 cggtaatgac cagccaccgc gtgagaagaa tcgtcatgaa tgttccctga aacatcgcca
    20281 tcttgctcct cgctagggcg tgtcgtccat cacggtcttt tttgacttca gaaagaccca
    20341 ggtgatgctg agcccgctca tccaggatgt tttcgtcttg accagggggc tgtcctcgaa
    20401 aaccgcgccc tgcagaaaat cggcgctgac aaacgcatga aagcgaaaag acttataatt
    20461 tttccccaac ccgacggtca gcgtggagcc gctgtagccc gcgcccgcat cgtaggccgg
    20521 acgccaggcc gtggcgtacg caggctccac gttgtaaaaa tattgatggt agccgcggtc
    20581 gccgaacatc ggacctgtgg agacgcccag atacaaaccc gtcccaggaa tcacgtcgcc
    20641 tttttcaaag ttcaatcggg gggaaaatac ccatccctca tggcgcacgg aacgaaaatc
    20701 ggttgaaaaa accgcgcgca ccggaaaggc caggctcagc cgatgccagt tttcgcggga
    20761 gtcgagcagt ttcacgcgca gcgcggggcc aaattcaaac gtgggatcca ggtcctccat
    20821 gccgcggcgc gcgtcattgt cgtcgctctt cacgggcacg gcgccgtagc cgctcgcgtc
    20881 aagggttacg cggtcggttt tgaaaagccg cgcggtgagc cttttgtctt ccaggcgaaa
    20941 aatgccgccc cggtaaacgg cgtagggaaa aggcagcagg tacggccgcg ttttgtccga
    21001 tccccggtaa tccggcatca tcagaaaacc gccgcctgcg cccacctccc acaaaggttt
    21061 ctgggcgcct gcagtctgtt gcggcagcat cagcaaaaca atcgccaaca gaattcgacc
    21121 atgcgtcatc atgaacgccc aaccctagcg gtcaaaagtc gtaaactgct cttttttcgc
    21181 cgcgcaaacc ggacattcct ccggcaatac cccgtccgcc acataaccgc agaccttgca
    21241 gacatgataa acggtttggc gctcttccat gaagtggttc atggcctgtt tgtagagctt
    21301 ggcgtgtgtt tcctccacgt cgcggctctg gctgaagtgc agaagggcct gcttgtcgcc
    21361 cgcctgctcc gccatcttta caaagcggtc ataggcgatt tccgccaccg ttttttccga
    21421 ctcgaagctt tccgcaaggt tttcttccgt cgttttgatc tccttgagca cgcgcagcgc
    21481 ccgtttgccg tgaatctctt ccgaaaaagc aatcacgcga aaaaggcgcg cgatctgcgg
    21541 atagccttcc tgatcggctt tttccgcgaa cattttcagc cgcaggacgg ctttcgcctc
    21601 gccgacgtag gcctctcgaa gcgcttcccg aatcttttca tccatcgctt accccctttc
    21661 ctgaaaaata cttattccgg cttgaactcg cttttatcgg cgccgcaaac cggacaggtc
    21721 cagtccgcgg gaagcttctc aaacggcgtt ccggggggaa ttccaccttc ttcgtctccc
    21781 tggtccggat cataaacata cccgcataca tcgcacacat actttttcat gattcgcctc
    21841 cttgaatggt cttatttctg ggacagtctt ttggccagtt ccgacgcaac ggtccgaccc
    21901 agctcatggc atttttcaag cgcttccgga cccggcacat gccggacgga aaccgtttcg
    21961 gccaccacgt ccactttcat tcctttgagg gccgtttcca gatctttcac cgactctccg
    22021 ctccagccga aagagccgaa caccgcgccg atcagattga ccggtttgag cccccggata
    22081 taggtcatga catccgccat ctgcggcagc atattgttgt tgagcgtgga cgaaccaacg
    22141 atcagcgccc ccgcctccag cagttcaaaa ataacctcgc tgcggtgcgc ttcgttgagg
    22201 gaaagaattt taacggacac acccgcctgc gccagggcct ccgcaatggc tttggccatg
    22261 ctttcggtcg aatgccacat ggtcgcataa accaccaccg ccttgttggc cggcttctgc
    22321 acagcccatt tcttataccg gtcgaggatg aaccccagat ccctgcgcca caccggtccg
    22381 tgatccggcg caatggtcct gatttcccag cccgtcgccg ccaccttttc cagcgctttg
    22441 agaactaccg gcgaataagg cagaacgatg ttggcgtagt atgtggcggc ctcgtattcc
    22501 aacatgtcgg ccggcagttc gtcggcgaag cgctcgaacg tcgccaggtg catgccgaag
    22561 gcgtcctgcg aaaacagcag gccgtcttta gccagaaacg taaacatcga atccggccag
    22621 tgaatcattc gcgtttccat gaaggtcagc tccatattgc ccagggaaag cgtctctccg
    22681 tcgcggaccg cctgaataac ataatcgtta tgaagaatct ctcccaggtt tttcacgccg
    22741 gcggcggagg caaacacttt tgagggccgg acccgtccga ccacttcaga gagacatccg
    22801 caatggtcct gttcggagtg attggaaata atgtagtcga ttttggctgg atcgatgacg
    22861 gatgcaatcc gcgccagcat ttcctccagg aagggcttct tgacggtgtc gatcagcgtg
    22921 attttatccg ccaggatcag gtaggcattg tacgtgcttc cgtggggcgt ggtgtagccg
    22981 tgaaaatcac gaatcgacca gtcgatggcg cccacccagt aaacatggtc gctcacttgt
    23041 atcgcggaat agatgtcggt catgaagggt cacctcggaa aatgatttgt gagggagtaa
    23101 tataaggttt accgccggat cgtcaaccct tatttgatca tggagcatcc cgcgtcaaaa
    23161 gccttcagat ttaaagcata tttttccgga gaaagggttt tgcgaatctc ttttgcgaac
    23221 atccgacggt cgatgggcaa aaggcccgtc ccggccagcg cgccgatcat gacgatattg
    23281 ctgagcacgg ggttgcccag gacaaggcct atggccgtgg cgtcaaagat ccagcaccgg
    23341 gccgtcaatc gcgcaaaggc ctcatgaatc gtttcctcgg aagggtagtc gcgttcgccc
    23401 gtgatcaccc ccatgggatc gatcgaacgg gcattggaga tgagcgagac gccgggattc
    23461 ccgtacaccg tcaaaacccg aagagcttca agcggctcca gcgccacgat cacatccgcg
    23521 ccgcctttgg gaatctgcgg acttttcacc gcgtcggggg aaatgcgcag atggctcatg
    23581 accgacccgc cgcgctgcga cataccgaac gtttcgccga tggtcacgac atagcccgcg
    23641 tccaccagca tgcccgccaa tgcccgggat gccatgacat tgccctgtcc gcccacgccg
    23701 gtgagaatca ggtttttggt gagtccggag tttttgtttt tcatacacaa ttcccgaaaa
    23761 tacaacgata cgctcctccc cgctccgtcg ggacggggag ccgtccaatc atccgccgga
    23821 agcgaaagcg gacgcgaccg gcgtttttac aatagccttc tgcggacaga tcgaagcgca
    23881 gacgccgcag cctgcgcaga ccacctcgtc aatcgccgct ttcttctttt tccggtccca
    23941 gacgagtgcc gggcagcgga aaatccttgt gcacagacgg ttgcagccgc aggcgtcccc
    24001 cagacacaac tcttcgtcca ccttcatctc gaatgttttt ttgccctttt tttcagggct
    24061 gagcgcgcag gcctggcgca gaatcagcac tttgagcaaa cctttttcgg ccagaaaatc
    24121 cgtcagaacc gcgccggttg ccgcaaggtc aaacgggtcg caaaccgtca ccttcgcgcc
    24181 gatggcccgg cagacggcag cgatatcgac ggccggaacg ctttccttga gggcgttcac
    24241 ctcaatgccc ggatgcggtt gaaaccccgt catggccgtc ccctgattat ccagcacaat
    24301 gaacgtgacg tccgcgcggt tgtgctgcgc gttgatgagc gccgggatca ccgcgtgata
    24361 aaatgtggaa tcgccgcaga ccgtcagaac gggttttttg aatccaaacg gcgccagttt
    24421 ggcaaaaccg ccggccagcc ccgtgccgga ccccatggaa tgaagcgtcg gcatcgtcca
    24481 gtagccgttg gccggcgtga gcgccgccat cgaatagcag ccgatgtcgc cgcagacgaa
    24541 cccttcgcga ctgtccagcg caatggcgtt gtggatgctc cagaaggacg cgcggtgcgg
    24601 gcagccggcg caaaacgtct gttcccgggg gggaagcatc gccgccgttt ttcgcgcttc
    24661 ttccagatag gacggcgaaa gtgcatcacg ccggatcttg aaaatcttcg ccagggcgtc
    24721 cgccacgata tcggggttga gttcccccgc aaaaggaagc gtcccgtcgt acttgccgaa
    24781 gaatgttttg ccctttgaaa gcttcgggtc tccggcggcc agaaccttga cgttttcttc
    24841 caaaaacgcc atcacttctt cgacgatcag aatctttccg gctttccgca gatggcccga
    24901 caccaggcga ggcggcaggg gccaggtggt cccgatcttc agaatgccca cccgcttttg
    24961 aaggcccagc atttcaaccg cctcggctgc gtagagagaa caggcgctgg ccgtgacgag
    25021 gagcagatca ggccgggcgg ggcccgtgta acagttgaac gggctttttt cgaaaagggc
    25081 ttgaacggtt ttgagctttt gctggcgaag cagatgtttg gccaccaccg gcgtgctgat
    25141 catcggcccg gtttgcgaat ccatgatcgg tccgtcgcag cggaacaccg ccttgcgggt
    25201 tttcgccggg aattttccgc aaatcacgtt tccgctggca tgcgacatgc gggtgacgga
    25261 gcgcagcatc acgacgttgc cgattgcttc ggaaagctca aaagcccatt tcgtcatgtc
    25321 cttggcttcc tgaaaatccg ccggctccag aagcggcatc tccagcatgc cggcgaagta
    25381 gcgcgactcc ccttcgttga cgctggagag cgcccccggg tcttcgcagg aaaccaggac
    25441 gaggccgccg cgggttcccg aagcggtcag gtgcagcaga aagtccgacg ccacgttcac
    25501 gccgttctgc ttcatgacgg ccatcgaacg cagtcccgcg aaggacgcgg ccgccgccac
    25561 ttccagcgcg actttttcgt tgaccgacca ctccacgtaa agattgcgcc cgcgggccac
    25621 cgtcgcaagc gattcgatca cctcggaaga aggcgtaccg gggtatccgg tcgccacgga
    25681 cactcccgct tccagcgcgc ctcgggcgat ggcttcattt cccagaaaca aaactctgga
    25741 cccggccggt gaaagcaatc tggtcatacg gatgtctcgt ctttcgtatc tcgtcgttcg
    25801 cgcgccatcg ggaccggttg gaaccaccca tgcgggcggc gcgacactcc cctacgtctt
    25861 taaatatttc ttcaccaccg tgtcgccggg attcaaatac agcaaattct gaggcgaccg
    25921 gcttttggcg atgatgcggg ccagcgcttt tttctttttc tccgccgccg cgtttttcag
    25981 ttcgtgaagc gcctcctgcg gcgtttcgcg aaattccaga atcccccggc tgcgcgccag
    26041 cgaaacaagc tttttccccg tttcgctgcg gacaatcacc tggttccagt tctgcatttc
    26101 cccggagttc cggccatagc gggcggcgcc gacggacagg tcggcaaact ccgccgtgga
    26161 atcgacgcaa tagcggcagg cctcgcgcac gcaggcatcc acctcggcca tcggcacatt
    26221 caccgggcct tccgccgtgt ggagctcgag aatatttttc cccgccggaa tgtccatgcc
    26281 ggtaagattc gccgcggaaa gttgataggt ttccagaaga ttctgaaaag gttcaagcgc
    26341 gagcgtccag ccgcaaaaca aaccgatgac cagcgaaagc gttttgccct tgtcgtatcc
    26401 atcaatcttt gccgtcctga ttttggccag cgcgagcgcc tggcagggcg tggccacgac
    26461 gccgattctg cggccttcgc cttccggcgc ccggtggaac gcggcgacgg tcggcgccgc
    26521 ccaaaagcgg cttcgggcat agtccgcaag cgctcccgcg tcgtcgatca aacggccttc
    26581 gggctcccgt tcactcagac ggccggaaac aatcgccgcg tcgatccatc cttcggacat
    26641 cgccagcctt aagagcgccg ttaccgtgcc gccgtgctga gagcgggccc gcagtcctgc
    26701 atccttcgcg cgcgcgatcc aaaactcctt caccggaccg atttcgtcgg tcgcatccaa
    26761 agcgtcaaaa agactttccc gaagaagcgg cagatcggaa ggcgttcggg ggcaaaaggc
    26821 gcggcacctg ccgtcgggca gatcacagtc gaaaagctga acggttcggt ctttgtacac
    26881 catctgatag gggcaaagcc ccacgcacgc tccgcagccg gtgcagaggc tttgttccag
    26941 aacatctttt ttcagcctgg actggctgct tcgtttcttt ctcatcaatg aacgcccttg
    27001 gccgcgcttc cgtccaccag cagattcaca ccgttgatgt aggccgcctt ttccgatgcg
    27061 agaaacgcca cggcgtttcc gatctcctcc ggttggccca gtcttccggc gggaatggcg
    27121 gcggccgttt ccgtaaaata ctgttcgacg gagatccgcc gcgccgcggc gcgcgtttgc
    27181 gcaagctcct cctgccttcg ggttcgaatg tggccggggc acacggtgtt taccgtaatg
    27241 ttgtattttg cgtactggtt ggccagcact ttggcaaggc cgccgatgcc ggggcgaacc
    27301 gcggaagaga gcagcagttc attgatgggc tgtttggcga cgatcgaatt gatggtgatg
    27361 atcctgcccc attcctgccg caccatcagg ggcaacacgg cgcgggtcag ccgcaccacg
    27421 ctcatcagcg tgaggtcatg gccccggagc cagtcttcgt cggtcaagga aagcagatca
    27481 cccgccggag gaccgcctgc gttgttgacc agaatatgaa ccgtaccgaa acgctgttcg
    27541 gcttccgaca ccagatggct gatgtcctgg ggctttgtaa catcagcttt cacgggaaga
    27601 acaaccgagc cgctttcttc acgcagctcc cgggccgctt gcgtaattgc ctcttcgcgc
    27661 ctggagcaga tcacgaccgt cgctccctcc agcgccaact ggcgcgccgc tgccctgccc
    27721 agtccctggc ttgccgcggc gacgatggct attttatttt gcagtcctaa attcataaac
    27781 cctccgggcc ttcccgccgc agcggtcgcc gcaaaagccg attacacaat gacgcgggag
    27841 accgtctcgc tcagtcctct ggcggcggtg ttcagttgct gggtgacggc cttaaactgg
    27901 gacagagaac cggtcaactg cgccgcgccg gcgttcagtt cggccatggc ctcgctgatc
    27961 tgccgggaac ccagagactg gaagcgcatg ccctcattga ccttgtcgaa ttccgggctg
    28021 agattggcca cccgggcgat gacctgtgta agctgcatcg tggcatcttc gacggagccg
    28081 accccacggg caacgtcctc gcgaaaatga tccatctccc ggacgccatc cacgaccgat
    28141 gcctgcattt cagcgatgat gtccgaaata tccagcgtcg aaatggcggt ttcattggcc
    28201 agccgtccga tttcccgcgc gacgacggaa aagccccttc cgtattcccc cgctttttcc
    28261 gcttcgatgg cggcgttgag cgacaggaga ttcgtctgat cggaaatttt ggtaatggtt
    28321 gcgatgacgc tgtcgatgct tgccgccttc tggcgcatca acgcaagctt ttcagaaatg
    28381 ctcgccgttc ccgctgagag gctttgcatg gcgctctgca aaccggaaag acttgcctgc
    28441 ccggcattgg ccagccggga cgcctcaccg gcggcatttc tcacgctctc catggttccg
    28501 gacagttcct gagacgtcgc cgaaatctgt ttggaagaaa cccccacccg cgccacgttt
    28561 ccttcgaggc ttttcatcgc tgcttcctgc tggcccgatg ccgaaacgat ggaggccgtg
    28621 gtcgcgttga gggtatcaac cgcctcccgg atccggatgg tctgctcctg aatgcttcgg
    28681 gtcattttgt tgatggagag gctgagcaga cccacctcat tgcggctgct gccggcgtcg
    28741 atctgcacgc gcagatctcc tttggcgacg ctgtctgccg cgccggtcaa cttctccatc
    28801 ggcttgccga ccagtcttcg cgtcaggaaa tagacgagca gcgcgaccgc gaccgcgacc
    28861 agaagcatgc tgatgaacaa tttgaacagg aaggcaaaaa tgccggaaag cacctcatcg
    28921 tagtttcggc aaaaaacgat ataccagctg tcctccatgt gaaactgggc cgtggtcagt
    28981 ttttccacat agtaggcctt gacggtgccc gcaaagtcgg acaaaaagga agattcgccg
    29041 gcggcgatcc tgtccgtgac gggtttcacc agggtcggcc ctttcttatt gaggatcagc
    29101 gatttttgcg gatgggaaat gatcatgccg tcttgatcga tcatcgagag gtagccggtc
    29161 gcgccgatga cggatttttc gacgaaaaac tccgtgaaat aatccattct cggcgcgaca
    29221 atggtcgcgc cgatcacctc gccgttatgc tcgatgggag cggcgacgac aaaaatggga
    29281 ttgcccctta gaatgctggg atagacctcg ctgatgaagt aaggttttcc cgcaaaaaca
    29341 ttcctgatat aactgaactc ggggccgcat ttcccgatcg tttttccgcc caccgtatcg
    29401 atgaaaaaat ttccatttcc gatgagtctc ttttccccgt ggacgtccac ggcaatcgtc
    29461 cggtccgggg caagctttac ggcaagggga atattttcat aataggggta gcgtgcgtgc
    29521 atttcggaca ggaatttctg cgcatccgct cgtttctgct ggtcctcggg ggaaagagcg
    29581 acggccataa tcctcgggtc gcgggcaatg gtctgcgccg tgcggatctg ctcctccagc
    29641 catcgcatga gcgcctgatt tgttttcttg gtaaccacct cggcgagctc gtaattgttt
    29701 ttcagggctt ccggtttgcc cagggtgtac gcgacaaacc cgaaggcaag aaaaagcagc
    29761 aagatggccg ggatcaacaa cagaagatac tgcgtggaaa tcttccgcga attgtatttc
    29821 ctgatcaatg ggttcagcat ccgcatcttc atttcatccc tgggttgttt tccgccgcaa
    29881 aacggattat ctgaataccc tattgccgat aatttatcaa catcttccgc taccacaacc
    29941 tgtgtctttt ccgacgcctt ttttccgcat cgaacctcat gatttcgctt gaaaggatga
    30001 agcaggccat gtaaatatac gggccgggga atttgaatat ggccgatttt aataagcagt
    30061 accgggaaaa attgacgacg tttgacctcg cggcgaaaac cgtcaaagac ggggatacgc
    30121 tgatccacgg cgtcaccatt gccgagccgc cgggcctgct ggcggccatc gccaaccggg
    30181 cccgcctggg cgacctgaac catctcaagg tttacacgtt taacgcccag aagcattttg
    30241 ccgaaacact gggaaatccg gatatcgccg atgtcgtcga ttcctacagt tggttcgtga
    30301 gcgaaggttc ccgcaacatg gtgcaggtcg ggttgacgca gttcgttccc gcctatctcc
    30361 atcaggtccc gaaaatcgta atggagacga tggaggtcga tgtggccgtg accaccgtcg
    30421 ccccgatgaa tgaagagggc tacttcagtt ttggcccctg caacggctac ctctccgtga
    30481 tggcaaccct gtgcaaaaca ttgatcgtcg aggtaaacga aaacatgccc ttcgtttacg
    30541 gcgacgccct gatacacatc tccgacatcg aaatgattgt tgaaaatacg gtccctctgc
    30601 tcgaactgct gcccatgcag ccaaacccgt tggatccgat catcggaaaa attgtcgccg
    30661 aaaccatccc ggaccgggcg gtgattcaac tgggcatcgg caccctgccc aacgcgatcg
    30721 ctccctacct ggacagtcac agggatctgg gcatccacac ggagcttctg tgccccggaa
    30781 tggtggatct gattcaaaag ggcgtgatta ccggcgcaca caaaaatgtc cacaccggca
    30841 aacatatttt ttcgctggcc tacggcggaa aagaaacctt tcaatatatg aacggggaca
    30901 aaagttttga aaattatccc agctcctacg tcatgaaccc ggccgtcatt gcccaaaacc
    30961 atcgcatgac ggccgtgaat gccattcttc aggtcgatct gctgggacag tgcaacgcgg
    31021 aatatctgga gggacgtcag tacagcggca ccggagggca gctcgatttt gtccgggggg
    31081 cctacgactc gccggatgga aaagccattc tcgttttata ttccacggca aaaaacgaag
    31141 aagtatcccg cattgtgggt cagtttcccg ctggaaccat ggtcacgacg ccccgcaatg
    31201 atgtgcacta catcgcaacg gaatacggca tcgtcaacct gaagggcaaa tcaaccaggg
    31261 caagggctct cgcgctgatc agcatcgccc atcccaaatt ccgggacgaa ctcatgcggg
    31321 aagccgaaaa catgtctctc atgtaagaag aattcctccc ggcgaaaaga aggatcatgc
    31381 ggaaacctcc tcctgttaca gccatgacct cctccatcgc ttcgcatccg gcgctggtca
    31441 ccggcgcctg ctctctggtt tccgtgcgcg ccatccagca gggcatcaag gcggtaaagc
    31501 gcttcctggg ctgcgacgtg cccgatgttt tcccgtgcat cgccgaaaag tacgccaaca
    31561 cgaccacgac cagccatttt ctggtgctgc acgattccat gctgcaaaac aaaatgcagg
    31621 ccggcgacaa catcctgctg atcagcggcg acttgggcat gctcaacgcc attcaggttc
    31681 tcgacgccat gatcaaaagc ggcgaggcgc aggtcggcat ggtcatcgcg agcgaaatca
    31741 acagcgaccg gcgtccggat tcctcctatc cctacccgac gagcggagcg gcgctgccgc
    31801 tgacgctgca aagcgcgtgg ggcggaccgc gcctgtcacc cggaaagaaa ggactgctcc
    31861 tggcctgcgg ttcgggaatc acggccggca cggctattta ttacttttag gcgccgtttg
    31921 agatctgcgc aacgccgaca aaccattttt aaggataatg ccatgaccaa agaagaactg
    31981 ctgaaagaaa tggatcaaat gcgtaaatcc atgaagcgcc ttcagaaatc cgaagcgagc
    32041 tgcaaagaag cggcggacct gttccggaaa cgcgagcagg aataccgcga gatggtcgaa
    32101 ctggccaaca gcatcattct ccgcatggac acttccggca ggattgtctt tgtcaacgat
    32161 tttgcgctcc ggtttttcgg cttttccaaa gaggagattg tcgggaagaa cctgatcggg
    32221 accatcgttc cgaaaatgga atcctccggg cgcgatctcg cggcctttat ggtgtcggtc
    32281 tgccggaacc ccggagaaca caccgtcaac gaaaacgaaa atatcaaaaa agacggcagc
    32341 cgggtctgga ttttatggac caaccggcct gtgttggatg aacggggacg gcttgccgaa
    32401 attatctgca tcggaaacga tgtgacgcaa aagaaggagg cggagcaaat cctgcaaaac
    32461 gcccacgacg aactggaaaa agaagtgcgg gaaaggacaa aaaagcttca gaagctcaac
    32521 gaagatctgc tgtttgaaat ggccgagcgc aaaatttccg aagaagcctt aaaagaaagc
    32581 gaaaacagat accgcaacat tgtcgagagc gcctttgagg gcatttttca gaccaccctg
    32641 gagggcaaat gcaccatggt caactccgcc ctggtggcgc tgctgggcta ttcctccgcc
    32701 gaagaataca ttgcggcgac ggcccgtccg gaaaacctgt atgtcgatgc gtcccgccgg
    32761 gaagaattga tcaggattct tcaaaccgac gggtatgtga agggatttga gacggaattc
    32821 tacagaaagg atgggagcaa actgccggta tccatgaatg tccgggccgt tttcgatgat
    32881 cggggcaatt tcatctatta ccagggaacc gtcatcgaca tcagcgcggg aatcacgctg
    32941 cggcgcgttc tggatgaaac caccggcgcg ctttccacgg ccgtggaaat ccgggacccc
    33001 tataccgcag gccaccagga gagggtaacc cgcctggccg aggcgatcgc cgccgaaatg
    33061 aatttcagcg atgaacgtat cagcgccgtg catacggcgg gtatgctcca cgatatcgga
    33121 aaaatctacg tgcccgcgga gttcctcagc cggccgggca agatcaccga taacgaatat
    33181 aacatcctga agcagcatgc ccaggaaggt tacgaaattc tgaaaagcat cgagtacaaa
    33241 tacccgattg cggaaattgt ctatcagcac cacgaacgaa tcaacggatc ggggtaccca
    33301 cgaaaacttt ccggggaccg gatcctgatc gaggccagga tcatctcggt ggccgatgtc
    33361 gtcgaggcca tggcctctcc ccggccctac cggcccgcac tcggaatcga taaagcgctg
    33421 gacgagatca ggaaaaacag agggatcctc tatgatgaaa acgtcgtcga cgcctgtctg
    33481 acgctgtttc aagacaaagg atttcaattc gggggggggt gacgggattc gatgcaacgg
    33541 ccggaaagga acagccaagg aaccggtccg tttctcaaga ctggagcgaa accattttca
    33601 agggacggcc gtaaatttcc tggccgagtt gcacagccac ctccttgacg gatgaaattt
    33661 cttttgatga aaatttcaat ttcgcatctg ttttttcggc cgtcactgaa aaaccggtgg
    33721 ccgcgttgca gccgtgcatc ctgcgggagg cgggaaaacg ccgctcgggt ttgctgtcgt
    33781 attccggcga ggtcgtatcc accagcccgt cgagcgatga aagcttgatc gtgtatcccc
    33841 tctttttcag gccttcggtc aggtgatcca gcatgcgggc aaccttgtcg tacggccgga
    33901 ttttttcaaa catgaaccag agggtgactc tttgatgcgt cgtcatggga ggaatatccg
    33961 gatccgtaag gatttcgctg aaggttccac cgtgtcccac tctgccgtcg aaatccccga
    34021 aaaaacgtat tccaaatgcg tccactctgt cgccgctggc cccgaattta aattttaata
    34081 cttgcatgcc ctctcttttc cggcggtaac ctcaaagcgt cttttttccc gccgctgcac
    34141 gctcatatca catgtcatag tcaatcacag cagttttttt gtccccgtta cggttttttt
    34201 gctgccgccc aaggtaggcc aaccgaccgg ccagcacagg aaggaaacag atcaaacgcg
    34261 cgaaaagatt tgaaatatca aaacaataat cctgcgtttg gcgcgaggcc gcatcgtcgc
    34321 gttcaaaatg acgcgcccgc agggattcca tcatcgggcc gcgatcttcc gtgagccggt
    34381 tcaacatgtc aatatcttcc ggcttcccgg aagaaaccgc ttctccggcc gtcaccagaa
    34441 gaaattcaag gctttcaaat aagacggtct cgtaaaaagt ctttttcgcg ctcttttcgt
    34501 cataccg
//
LOCUS       DAOSRM010000011        32265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1526_length_32265_cov_99.933561, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000011 DAOSRM010000000
VERSION     DAOSRM010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 32265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32265
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_32265_cov_99.933561"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..601
                     /locus_tag="PLA58_02330"
                     /pseudo
     CDS             140..601
                     /locus_tag="PLA58_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            623..4588
                     /locus_tag="PLA58_02335"
     CDS             623..4588
                     /locus_tag="PLA58_02335"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOR61662.1"
                     /translation="MGTSPCKAHCPTHISVQGYVALIAEGKFKEALKLIKQDNPFPIV
                     CGRVCNHPCESACMRGKVDEPIDIMHLKRFVADLDLNSDTRYLPEKKESKGKKVAVVG
                     AGPSGLTCAYYLAIEGYDVEVFEALPVAGGWMAVGIPEYRLPKNVLNAEIKVIQDLGV
                     KIHLNTRIGKDISFDKLKSDYSAVFIGCGTMKSSKLNIPGEDMQGVVHGVDYLMKINL
                     GEKVALGDRVAVIGGGNVAMDAVRTAVRTGSKNVFILYRRTRAEMPASPEEIEEALEE
                     GIEMKFLVAPKRVVGKDGKVTGVECTRMELGEPDKSGRRRPIEIKGSEFIVECDAIVP
                     AIGQEADLSFITKESGVSINKWNNLDFDKITYATNVAGVFTGGDVSTGPQTVVKAVYS
                     GKEAAKSIDRYLQGQDMKAGREKDWTKDLADKADLSNVPKVPREKYPNMKPEERRTSF
                     QEVGIGFSEAQAIAEAHRCLACGICSECYQCVEACIAKAIDHDMKVEEETIEVGAVIA
                     SAGFEIFDAGKRGEYGYGIYKNVVTALQFERILSASGPFFGHVQRISDGKEPKKVAFI
                     QCVGSRDVSCGNSWCSSVCCMYATKEAIIGKEHAKGLEPTIFYMDIRAHGKDFDRFVN
                     RAKDEYGIRYIRSMPSAIKELQQSNNLLIKYTNPDGSFTEEEFDMVVLSVGLMPPKEA
                     KKLSASLGIELEEHGFCKTALDNPVQTSRPGVFVCGAFGGPKDIPETVMEASAAAACA
                     EGLLASKRGTMITPVENPEEKDMRGQGVRTGVFVCHCGINIGGVVNVPEVRDYAATLP
                     TVVYAADNLFTCSQDTAVKMAEVIKEKDLTRVVVASCSPRTHEGLFQENCEKAGLNRY
                     LFEMANIRDQNSWVHMHEPEKATEKAKDLLRMAVAKAQYLKPLKPGQLSVNHQALIIG
                     GGLAGMTAALSLADQGFASTIVEKEDRLGGNYNHLYKTLEGLDAQAHLKGLHEKVYKN
                     PLITVVTGAAIEKIEGFIGNYKTTVKSREGEKVFEHGVVLVATGAYENKPKEYLYGEN
                     AKVKSQRELEKMIYEKDPKLASVKNIVMIQCVGSRDKERPYCSRYCCGEAIKNALELK
                     AADPSRDITILYRDIRTFAFKEDYYKKAREANIKFISYEENRKPEVAAAGDKVEVKVL
                     DPILNETVSLPADVVALSIGVVPNPENAAIGQMLKIPTNQDGFFLEAHVKLRPVDFAT
                     DGVFMCGMSHSPKYSDETVTSANAAVSRACLVLSLDFIEAEGKTAYVNKERCSACGLC
                     EINCPYSAIRVNPAEGCAEVNAVLCKGCGVCTASCRMNAVDLNGFNNEEVLAQIYNIN
                     N"
     gene            4633..5073
                     /locus_tag="PLA58_02340"
     CDS             4633..5073
                     /locus_tag="PLA58_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4849..4851,aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOR61663.1"
                     /translation="MAENTATKEWEPKFVAIVCNWCTYAGADLAGISRIQYPTNVRII
                     RVPCTGRINPFYIVKALQTGADGVLVSGUHPGECHYISGNLVARRKFATLKSFLNYIG
                     VEGDRTIFTWVSASEGDRWAQVIKGATEKIRALGPANKMVKQIG"
     gene            5116..6126
                     /locus_tag="PLA58_02345"
     CDS             5116..6126
                     /locus_tag="PLA58_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR61664.1"
                     /translation="MENIETKLREEAKRLLLEKKAEVIVGYEKGTLPLTATPCFITKP
                     EEADKLVWNNLCTQNLAKFVHDLISQHKNSQKRVKPEDRKKKVIGVVARGCTTRSLII
                     HLQERQYERGDIFIIGVPCDGYVDGKGLKVKIGGADIIDGAVEGGQIKMKTSDGDKTL
                     ALKDVLADCCVTCRFNNPIISDVMIGSAAPPMNPDAEYNEVKEFEAKAMEERWEYFTK
                     EMAKCMRCNACRQACPSCYCPTCFVEQSQPQWVGIGEDQTDTQVFQFMRLYHMVGRCV
                     DCGSCVAVCPMGVDLRKFLKKLDKDCFEFFGNRAGSGMEDMPPLGKFDEHHDKGDFVY
                     NP"
     gene            6150..6677
                     /locus_tag="PLA58_02350"
     CDS             6150..6677
                     /locus_tag="PLA58_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR61665.1"
                     /translation="METFLEEVNKKIHGVPLQACYHCRKCTAGCPAASYMEFNPNKVI
                     KMIQNGQKDRVLSSSTIWLCLSCETCITRCPNQVDIARMMDVLRQMAIEEGRAAKEAN
                     VLKFHEAFLSSIKFGGRVHEMLMTVHYKLKSGDLFGDVDIAPSMFLKGKLSLLPPRTK
                     DLKSVRNIFEKTQSK"
     gene            6749..7597
                     /locus_tag="PLA58_02355"
     CDS             6749..7597
                     /locus_tag="PLA58_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOR61666.1"
                     /translation="MKVTYYPGCSLHGMGKEYDQSMKAVSRALNIELKEVDDWSCCGA
                     SAAHNTNFDLSVALPARNLIAAEKDAMDVMVPCAACFNRFKMAEHHLKADKDLKARIE
                     GVLGAKYQGGIAIRNPIDVIYNEIGMDALAAKVVKPMTGLKPVSYYGCLLLRPPEVCQ
                     FENYENPFMLDKIMRTIGADVKNWSYKTDCCGGSLSVSKTPIVTNLCDKLMAAAKEAG
                     ANCVVTACPMCMANLEMRPSANMKMPVFYFTELISLALGLPGSQETFKTHLTDPQPLL
                     NQLRLV"
     gene            7668..8690
                     /locus_tag="PLA58_02360"
     CDS             7668..8690
                     /locus_tag="PLA58_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR61667.1"
                     /translation="MEKRLIKKDTLAGIAKKMAGDFLVWAPVKKEDNVLFEPMGKDGE
                     PFFAYLNSKNAPKNVFFPHTETLMKYTRTPKGMVFSSEVNRADQSVLFGVRPCDAHSF
                     VLLDKLFDQEKYKDAYYIEKRKSTTVIALACVKPPYATCFCTSVDGEPVSADGADILL
                     TDIGGDYLVEFITKKGEKLASYFGDAKADAAADARKAEIAAQAKAAVTSKIPAHEVKP
                     ILDVNFDNPFWNTIHGKCLACGTCTYMCPTCHCFDISDEVKGADGIRLRSWDSCMFPL
                     FTKETSGHNPRPSQKERWRQRTMHKFKYYVDMFGAISCVGCGRCVMSCPVNIDIRKVV
                     EDISKL"
     gene            8705..9544
                     /locus_tag="PLA58_02365"
     CDS             8705..9544
                     /locus_tag="PLA58_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOR61668.1"
                     /translation="MQNPYIPYPVVVDKIITEVDTKDIKTFRFKFVNPEDEQKYAYIP
                     GQFGELSIYGKGESPIGIASSPTQKGYVEFTVQKAGVVTSALHEMEEGEMMGIRGPLG
                     NSWPLQFLEGKNIVIVGGGFAFTTLRSLINYMLHEDNRKRFGNITVIYGARNPGLLLY
                     KDELTEWGKRTDLNLNVTVDKGDATWKGREGFVPAVCKEVAPSKENAVAVICGPPVMI
                     RFTLPVFFELGFSKENIITSLEMRMKCGIGKCGRCNVGSKYVCKDGPVFSLAELDKLT
                     PEY"
     gene            9716..10891
                     /locus_tag="PLA58_02370"
     CDS             9716..10891
                     /locus_tag="PLA58_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOR61669.1"
                     /translation="MPQTVTPYLEMSQAIMEAGGEALKKCYQCGTCSGTCPWTPITHF
                     NIRKLVRYGQLGLDGIEDFMWGCSTCRFCVERCPRGVELIDIVTAIRNVYSGGGLLPQ
                     SLRAFVGSLSARGNPWSGDPAVRNDWAKGKYPVYKPGMDYLFWTCCTVCYDPRNTRLA
                     KAAAEILNLAGASWGMPSADVNCCGESLRKVGDAELFGRLKGKNLDYFKSNEVSKIIT
                     VSPHCLATFNNDYGQDYDVTHLSELMAQWIKDGKLVPKKDFGGLTVTYHDPCYLGRHS
                     KVYDAPREILKALPGVEFVEMERNSEESMCCGGGGGGLWTEKAKGERLSDLRIEEAMG
                     TGAAVLATACPYCITMFEDSARTLNADGQVQIRDVTELLLESLEINIDEPVADTCAL"
     gene            11404..13353
                     /locus_tag="PLA58_02375"
     CDS             11404..13353
                     /locus_tag="PLA58_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOR61670.1"
                     /translation="MSRIGVFVCHCGLNIAKTVRVSELAEFAATLPDVVVAKDYKFMC
                     STPGQEMIANDIREHRLDRVIVTACSPLMHEQTFRKVLAASGLNQYLLTIVNIREQVS
                     WVTHNIDEGTSKAKALLNGAVNRSRLLMPLTSRVIDVNPDVMVVGAGIAGIQASLELA
                     NTGRKVYLVEKNSTIGGHMAQFDKTFPTLDCSACILTPKMDSVLQHKNIQLLTSCEVS
                     EVKGFVGNFDITIRQKARYVDHDKCNACLACTEKCPGKSVSEWDEGLAKRKAIYIPFP
                     QAVPQKPVIDREACTFFKKGKCRLCEKVCEQKAIDFEQQDTFSTVKVGAVIVATGYDL
                     MDTRDLIQYGYGKYPGVYNALEVERLFNSAGPTGGKVILRDGKEPQAIAILHCIGSRD
                     KNSYEHCSRVCCMYSLKFAHLFKEKTSADVYHFYIDIRAAGKGYEEFYNRIIEEDVKF
                     IRGKGARVAKSADEPDRLMVEAEDTITGKFIRLPVDMVVLSPAMIPRSNAKDIARLFN
                     LSTDKQGFFMERHPKLAPLSTMSEGVFIAGVCQSPKDIPDTVAQANGAAAEALTIVVK
                     DKMELEATTVMVNPASCCACQNCVRVCPYGAPFFKEDKGVSEINEALCKGCGLCASVC
                     PTGAIIARHFTNDQVLAEMEGLMEF"
     gene            13508..13921
                     /locus_tag="PLA58_02380"
     CDS             13508..13921
                     /locus_tag="PLA58_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOR61671.1"
                     /translation="MSFEPKILGIACNWCTYTGADLAGTTRLQYPQNLRVVRVMCSSR
                     INPSFIFRAFQLGADGVLVGGCHPGDCHYNTGNLYARRRLAVTKKILEFAGLEPGRFR
                     VEWISASEGNKFAEVVTEFTGQIKALGPQTKFKIS"
     gene            13938..14804
                     /locus_tag="PLA58_02385"
     CDS             13938..14804
                     /locus_tag="PLA58_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR61672.1"
                     /translation="MAVSFQQIEEKLKTEARRLLESGQVSLVLAWGRGFDEKHPMPYV
                     AKGASDVDGIVFNEFCTHNLARYLVRYPRGTKMAVAVKAADSRAVVQLIQEDKVKRHD
                     LVILGIPVYGIKNPKTGEIIDGKTTCGLYNPVLYDILIAEEVHGQPVSSPYAVLAEYE
                     AMDQDARFAFWKKELDRCIRCYACRKACPMCYCDPCFIDQNKPKWGEKSPETPGNLMY
                     HLTRFHHLAGRCIDCGECSRACPVDIPLYLFHKKVAKECEEMFGQAAGMSIEDKPVLV
                     NFRMEDSDKILE"
     gene            14817..15854
                     /locus_tag="PLA58_02390"
     CDS             14817..15854
                     /locus_tag="PLA58_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR61673.1"
                     /translation="MLKKTAIHQLSDLTSALMGKGTLVAPVREDAGFNFREISDAKQV
                     DLNFHNTIISPKSVFFPQTEDFIRYRTGRPLQEAETVEPEGKPLILFGVRPCDVKSFE
                     IMDIHFSCTGAVDPYWQARREAATLIGYAFDVAAPAEPEEFYQTLGIGAADPEGSDIF
                     MVRRGDDLILKSITGKGEALLAGIEGLAGASADDGKFFDDTVAAGRNFKTRFTCVDAK
                     AITKKLEDLFHNTDFWEKASNACLSCGVCTFACPTCYCFDICDETLFGRGTRRRVWDA
                     CMFTDFTLEASGHNPRTKVYQRLRQKIGHKYSYHVRKYGVISCVGCGRCTRHCPVGID
                     IFSIVEEAMKA"
     gene            15905..16765
                     /locus_tag="PLA58_02395"
     CDS             15905..16765
                     /locus_tag="PLA58_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOR61674.1"
                     /translation="MRSTNPYIPMPVEIVKITDEVDTHDIKSFRFKFLNAQDEARFQY
                     LPGQFAELSIYGKGESPIGIASSPTEPGYVAFTVQRAGAIVPGLVTAALHDMEEGGRI
                     GLRGPLGHPWPIEFLEKKNIVVVGGGFAFTTLRSLVTYMLHENNRSRFGDITVIYGAR
                     TPGLLCYKDELAAWEKRSDIKTIITVDKGNETWKGREGFVPAVCKDVAPSPANAVAVI
                     CGPPVMIKFTLPVFFELGFSKENILTSLEMRMKCGIGKCGRCNVGYKYVCSDGPVFSL
                     AELEELPRDF"
     gene            16807..17208
                     /locus_tag="PLA58_02400"
     CDS             16807..17208
                     /locus_tag="PLA58_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61675.1"
                     /translation="MKKVMAILTMIVFVAGPALAADKAFQKADKNKDGKITSQEYLNA
                     TSKAFDELDKNRDGVLTKEEIQANDKIDADKLIRETDANRDGKILKKEYQKAAKQRFH
                     SMDKNKSGHIDYNEWASDRSDLYSPFNLFSF"
     gene            17390..19573
                     /locus_tag="PLA58_02405"
     CDS             17390..19573
                     /locus_tag="PLA58_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOR61676.1"
                     /translation="MRYTVQPLQQGYTNQILSINVTTGKMEVRPLPPEVRNFFIGGRG
                     LGLYLLHEQVTSRTKASDPENPLIFSPGPLGGIPQFPGTSKCMAISLSPLTGIPGVSN
                     FGGHFGAYLKYAGFDALTVAGIAPEPVMIVIDGFRREISIVGVSGDDQVFHLEKTLVD
                     RFLREGHEKKDLAFLTTGIGAEHTSFGCINSHYYDATKPVEGGKGLFRTKQAGRTGLG
                     SVMSQKLVTAIVVLAAFPRGENPYGAADWNRVKEAGAKLHGVVRDVDPQALKMHLKGS
                     AGLISFMNKEEYQSLPVHNYQRGSHPRAGEICGKVYAERLFEHRGMDGCFPGCNLRCT
                     KGGWVMLTSGEHAGRRVWVDGPEYETAAGFGSNLGMWHPEFVMEANWHCDNYGIDTIT
                     TAVIMAFVMECCQRGYLTKEDTDGFCLTWGDEQAALQFIHDLAYRRTDLARAAALGMV
                     GLTDWVSLKYALRTGKAKPLEELQTFAMQTKGLPFSLYRTHRSLSMQASYAAASDIGA
                     HHAAAWLVKVDLLGAFPTFESKARALITYPRVRLGNDNLGLCKLPWVDVLNPDSMKEN
                     HTDIYINPASQELYADFYNGMLGTELTWEQIFEQTDRDINLQRVLNVLSFGATTGERD
                     WIPDRAIGPTEDTLYELEKEYNDRQLSEILQWPLPEIQQMPTGEKRAALMRHRKEDLR
                     QLILVYYRERGWTDAGVPKVETLQQIGLWRFLTDEARAIITRLNG"
     gene            19601..21352
                     /locus_tag="PLA58_02410"
     CDS             19601..21352
                     /locus_tag="PLA58_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR61677.1"
                     /translation="MLQNQVWQPLPGIRQAEIYPYLRKPDLLSSNSCVIRTPQQILLI
                     DAGALAAQTADLNRILAECQRERTRPVVVYLTHCHLDHSLEVGRHRQIMTAAPVWIAV
                     QEQGADYLSLGDPAKTIAELYGVAFPSLRPDIRLLTETDMRRKAPRSISLQPGVNMRI
                     TTEAIATDLKEPLFQQTISIGGGDVLELYSAPGHSPDSVCIRVGEILFIGDLLAAANP
                     MVAGIAGWHREHLLDTQRHVQWLLDHQPITLCYPGHGGLIPSEKARDILGRLQRKTLS
                     LGDVSRMNEERLFQITDYALELIDEAEEVFSSIAGRLLYVAYQLERLEEEEAACRCRD
                     AMPMERIDACLLEFRKLCRRLESGKIRRVEFAHGALAIVEQIKGLFDPRPLTAILPQP
                     MIHRGTSLLLDFIGIANGRRNLEEFIPTDVNAVIDDVLRAGRETPHLDASVLDYVEDE
                     ARFLAALVLRIGHEPAAARPPVHFLPHKSLPYVSVAPGRFSDTLLTFLDWLAQTHPPS
                     IRIATGMHRGGPFVTIAPAGWGDAAPTPHRKKKIDSFARRFRMCGLVLHAKKEGFRLT
                     LAEGPDAADVSAPVDLH"
     gene            21603..22460
                     /locus_tag="PLA58_02415"
     CDS             21603..22460
                     /locus_tag="PLA58_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOR61678.1"
                     /translation="MEIKKICVLGAGLMGNGIAQICAQAGYEVALRDIEQRFVDGGMN
                     TIKKNLARDVEKGKMTQAHMEAVLGRITPTLDLKQAAAGADVVVEVVIEVMDVKKKVY
                     AELEAVVPEHCLFFTNTSGLSITEMAAVTGRPDRFIGTHFFNPVPVMRLLEIIRGHQT
                     SDETLKTAQEWGRKIGKEVITVKEAPAFVVNRILCTMLNEAFFVLDEGLASAEDIDKG
                     MVLGCNHPIGPLALADLVGNETLLRVIEGLQRELGDKYRPAPILRKLVRAGNFGRKTG
                     RGVYDYGKK"
     gene            22536..23678
                     /locus_tag="PLA58_02420"
     CDS             22536..23678
                     /locus_tag="PLA58_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR61679.1"
                     /translation="MNFELTEEMRMLKEMAYKFAVAEIAPFSAECDEQEKYLPEIRKK
                     AAENGLVGAWIPEAYGGSGAGILGNAIITEELSKIDMGIGLNIVASGFGCESILNYGT
                     EEQKKTYLPPICEGVQVCAGAYTEPNAGTDVSGYKTRAVKDGSDYVINGNKMFITNGT
                     VCDFMVAQCITNPEQKKHNSFSLIIVPADARGVTRNKIHGKMGIRASDTAEIAFEDVR
                     VPQSNLVGKEGNGFRQLMHFFDITRVMVSGQALGLAQACLETSIKYVKERTAFGAPLG
                     SFQITQMKLTEMAIRIEALRGLVYKAAWLIDQGKPDYTLAAMAKFYGGQTAVFCANYA
                     VELHGGYGYIEEYPVQKWYRDAKILELYEGTKEAEIMTIGRALQTR"
     gene            complement(23681..24190)
                     /locus_tag="PLA58_02425"
     CDS             complement(23681..24190)
                     /locus_tag="PLA58_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61680.1"
                     /translation="MRKSGMISAALLAAVFVGLFTGCSGKLLKDMGSFKPSIEATENF
                     KKFIVRSDYNYFISGSDVYPVAIFGLQKDVVIDSDEDLWKKIEPKTEIMADLVTNMQR
                     RLNECCLQSPHGLDILDNQGRKIGEWYSMLGLIIGIKIKEGGKVVIYPPSDTDEVKKY
                     QDRTSGKAR"
     gene            complement(24326..24550)
                     /locus_tag="PLA58_02430"
     CDS             complement(24326..24550)
                     /locus_tag="PLA58_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61681.1"
                     /translation="MPEAIREERRTVGIGQFENSVLSEAEDLLMGVLSIGKAARDVNH
                     ELIPHGSTAARGNPKKRSSPFLKKGFENYV"
     gene            24613..26403
                     /locus_tag="PLA58_02435"
     CDS             24613..26403
                     /locus_tag="PLA58_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOR61682.1"
                     /translation="MKNSLELGLIGNCRIGALIDERGEIVWSCLPRFDGDPVFCSLLR
                     EHADGEGSGYCAVELIDQVEAAQFYLPNTAILVTRLTDRHGASIEITDFAPRFHQYGR
                     TFMPMMMIRQIRRIHGTPRICVRVRPVCEYGGQSCTVTHGSHHIRYVAPSWILRLTTD
                     ISVTAVLQEIPFFLETAATMILGPDETIPDAIGDLSRRFLDETVGSWRNWVRDLSIPF
                     EWQEEVIRAAITLKLNTFQDTGAIIAAMTTSIPEAPDSGRNWDYRYCWLRDSYFVVNA
                     LNRLSTTNTMERYLNYLINVAAGTPNGRIQPVYGIDGRAKLDERDVDSLPGYRGMGPV
                     RIGNQACEQIQHDVYGSAILAVAHTFFDRRLAHRGGAALFSRLEPLGEAAMAVHDQPD
                     SGPWETRNTLRVHTFSSLMCWAACDRLARIAVYIGLSERAACWQSRAERIHATICRRA
                     WNEKKQSFVGAFDGDSLDASLLLLHDIGFLSDDDPRFVRTVAAVERELRRGDYIYRYV
                     ETDDFGVPQNAFVVCTFWYIYALAALNRGEEARALFENLLSRRSRHGLLAEHIDPRTG
                     DPWGNFVQTYSMVGLINSAIRLSRRWDSAF"
     gene            26417..28669
                     /locus_tag="PLA58_02440"
     CDS             26417..28669
                     /locus_tag="PLA58_02440"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOR61683.1"
                     /translation="MSLQSIKSSRSLRLTLRFVIPLVLAMALLAFAVLPLVDKLTLHW
                     SVRDMDIRSKLVTSTIQDPLAELLEQGNKRKIQSLLSRATQDERLFALGYCDIQGKLL
                     FKTRDFPDILGCSVPDPSVRKASSLLELPQGLVHVAVNPFLIEGLPMGSLMVLHDMSF
                     VRQRSEDTRKYLLVLFAVLSLVTSVITVLIAHLSWRGWMTGVRALLRGEGLIKPFAKS
                     SSSELQPLVGDLRALLRTMDAEKRIADDLTISWNPDSLRSLLFKQLAGEQILVVSNRE
                     PYIHMKGKEGMEVQRPASGLVTAVEPVMRACSGIWIAHGSGTADREVVDKKDHVRVPP
                     EHPEYTLRRIWLTEEEERGYYYGLANEGLWPLCHIAHVRPVFRTADWNRYVAINQRFA
                     DAVVREAEREDPVVLVQDYHFALLPGMLQQVLPKATIITFWHIPWPNPESFGICPWRE
                     EILRGMLGSTILGFHTPFHCKNFLETVDRYLETRVNYASSTISHGGNLTLVEAYPISI
                     QWPSPWQDTQPPVARCREDIRQELNLGRDILVGLGVDRLDYTKGILERLRAVECLLET
                     HPDLVGRFCLIQIAAPSRSVLEEYKAFESRVRALAERINAKFSRNGYQPVHLKVEHHE
                     PRNINRYYRAVDVCLVTSLHDGMNLVAKEFVAARDDERGVLVLSRFTGAAHELYEALI
                     VNPYHIEQTADALYQALTMPEFEQQARMRSMRSMVRDFNVYRWAARMLLDAARIRRRE
                     KLAERIGVAL"
     gene            28666..29445
                     /gene="otsB"
                     /locus_tag="PLA58_02445"
     CDS             28666..29445
                     /gene="otsB"
                     /locus_tag="PLA58_02445"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOR61684.1"
                     /translation="MRLDLPAVYLFGRTACSVLDRFLDRSTLFAFDLDGTLAPIVSDP
                     DAAGISGAVREELSVLAGMAPVAIITGRSRPDALRHLAFAPRYLVGNHGAEGLSGWKA
                     REKTFVRTAQGWQCQLDTLIPVEERRGIVVENKGSTISIHYRHAENRNKAHAIIRRAA
                     GRLVPPPRLVRGKCVENLIPEGAPDKGAALKLLMRQAGCAKAFFAGDDETDEDVFRLN
                     HQRILAVRVGRKNDSGARFFLRDQSEIVRLLREINRILGRT"
     gene            complement(29470..30609)
                     /locus_tag="PLA58_02450"
     CDS             complement(29470..30609)
                     /locus_tag="PLA58_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR61685.1"
                     /translation="MAFEITSEQEMIRLMARDFARKELEPMAAEWERQGIFPMDAVKK
                     MGGLGLLGMMVPPELGGSGAGTVAYSLALQEIAYACASSAVTMSVANLSTEPLLRFGS
                     PEQKEKWLMGLAEGSLLGAFALTEPGAGSDPGSMTTTAVLRKDKYIINGTKVFITHGQ
                     YADVVNLIARTGQERGTKGLSAFIIEKGMPGFKIGTKEDKLGLRASNTVELVFEDCEV
                     PAKNLLGNPGEGFKVAMRALDSGRIGIASQAVGIARAALDEALSYTEQRRQFGKTINS
                     FQAIQFMIADAATRIEAATWLTLAAADRKDRGLKFTKEASMAKLFASETANKAAYMAL
                     QVHGGYGYTKEYKVERLFRDARATTIYEGTSEIQRVVIAREVIKT"
     gene            complement(30621..31169)
                     /locus_tag="PLA58_02455"
     CDS             complement(30621..31169)
                     /locus_tag="PLA58_02455"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOR61686.1"
                     /translation="MAKVKEHKKSFGQSIKAYRESRKLSIKDLAHETGYPADLLEKVE
                     KDETTAPVALVLQLSRTLKVDVAQLNGDENKKASSRVKSQKKRAGSYAYTQLTKSGAD
                     KHLGAYLVTIEPNTAHEGVEYHHEGEEFVYVLKGKLSISVGKNTSLLSPGECIHFNSA
                     LHHALSNPSSDKTKLLVVLYIP"
     gene            complement(31195..31584)
                     /locus_tag="PLA58_02460"
     CDS             complement(31195..31584)
                     /locus_tag="PLA58_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOR61687.1"
                     /translation="MIENTIATWHKLVKERDAAGLDGILAENVVFHSPVVHTPQAGKP
                     ITTLYLTAALHVLNNDTFQYLREVISGNNAVLEFLTVIDGISINGVDMITWGEDGRIT
                     DFKVMIRPLKAINLVHKMMGEMLQKIK"
     gene            complement(31600..31950)
                     /locus_tag="PLA58_02465"
     CDS             complement(31600..31950)
                     /locus_tag="PLA58_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61688.1"
                     /translation="MSEADEPRITNIKINPDNLYKEESFTDLTFATIRRLSPVNIDGS
                     PDESREPLFTGMAQLMSPNGPIPVQCLIEGAKTLPEAAAKLPDAIEKAVKGMIAEAQE
                     MERQEASKLIVPGQ"
BASE COUNT         7181 a         8841 c         9427 g         6816 t
ORIGIN      
        1 gtcaatgaat acaaggcctt gtattctaaa atccaaatac atttatcttc tgcgcttgac
       61 acgataaata aagtgtgtta gccgacggac gctgatccga caatgcgtcg gacggatttt
      121 tacggggctt cttatataaa ttacgaggtg cgaacacgtg gtaaaagata acgaaaaagt
      181 tggtgcagta atggtggtcg gcgcgggtat cgccggcatt caggcctctc tcgatctggc
      241 caattcagga atgaaagttt atctggtgga aagcgggatc agcatcggcg gcgtcatggc
      301 ccagctggac aaaaccttcc ccacaaatga ctgttctgcc tgtattctgt ctccgaagct
      361 ggttgaagtc ggacgccatc cgaatgtaac gatcaaaaca aggcgcacgg tagaagccgt
      421 ggaaggcgag ccgggaaaat tcaaggtcaa actcacctcc gccccccggt acatcaacct
      481 ggacaaatgc acgggctgcg gcgattgcgc caaagtctgc cccatcacgg tcaaagcgga
      541 attcaacggc aatttaagcg aaagaaaggc cgtctatcgg cacttcccgc aggccattcc
      601 cagcggcttc gcgatcgaca aactgggcac ttcgccctgt aaggcccact gcccgacgca
      661 tatcagcgtt cagggatatg tggcgctgat cgccgaaggc aaattcaagg aagccctcaa
      721 gctgatcaag caggacaacc cgttccccat cgtctgcgga cgcgtctgca accatccctg
      781 cgaatccgcc tgtatgcgcg gcaaggtgga tgaaccgatc gacattatgc acctgaagcg
      841 ctttgtcgcg gatctggatt taaacagcga cacgcgctat ctgccggaaa agaaggaaag
      901 caagggcaag aaagttgccg tggtcggcgc cggcccctcg ggcctcacct gcgcgtacta
      961 cctggccatt gaaggctacg atgtggaagt ttttgaagcc ctgccggtgg cgggcggctg
     1021 gatggccgtc ggcattcccg aataccgcct ccccaagaac gtcctgaacg cggaaatcaa
     1081 ggtcattcag gatctgggcg tcaaaatcca cctcaacacc cgcatcggca aagacatttc
     1141 cttcgacaaa ctcaaaagcg attacagcgc ggtattcatc ggctgcggca caatgaagag
     1201 cagcaaactc aatatccccg gcgaagacat gcagggcgtg gttcacgggg tggattacct
     1261 gatgaagatc aacctcggtg aaaaagtcgc gctcggcgac cgggtggcgg tcatcggcgg
     1321 cggcaacgtc gcgatggacg cggtccgcac ggcggtgcgc accggctcga agaatgtttt
     1381 cattctgtat cgccgaaccc gcgctgaaat gcccgcttct cccgaggaaa tcgaagaagc
     1441 gctggaagaa ggaattgaaa tgaaattcct cgttgccccc aagcgcgtgg tgggcaagga
     1501 tggaaaagtc acgggcgtcg aatgcacgcg catggaactg ggcgagccgg acaagagcgg
     1561 ccgccgccgt cccattgaaa tcaaaggctc cgaattcatc gtcgaatgtg acgccattgt
     1621 tccggccatc ggccaggaag cggatctctc cttcatcacc aaggaaagcg gcgtttccat
     1681 caataagtgg aacaacctgg atttcgataa aataacctac gccaccaacg ttgcgggcgt
     1741 tttcaccggc ggcgacgttt ccaccggtcc gcagacggtc gtcaaggccg tttattccgg
     1801 aaaggaagcg gcgaagtcga tcgatcgtta tctgcagggt caggatatga aggccggtcg
     1861 cgaaaaagac tggaccaaag acctggcgga caaagcggat ctctccaacg ttcccaaagt
     1921 tccccgggaa aaatatccga acatgaaacc ggaagagcgc cgcaccagct tccaggaagt
     1981 cggcatcggc ttctccgagg cccaggcgat tgcggaagcg catcgctgcc tggcctgcgg
     2041 catctgctcc gaatgttatc agtgcgtcga ggcctgtatc gccaaggcca tcgaccacga
     2101 tatgaaagtg gaagaagaaa cgattgaagt cggcgccgtg attgcgtcgg ccggctttga
     2161 aatcttcgac gccggcaaac gcggtgaata cggctacggc atttacaaga acgtcgtcac
     2221 ggccctgcag tttgagcgca tcctgtccgc gtccggcccc ttcttcggcc atgtgcagag
     2281 aatttccgac ggcaaggagc ccaaaaaggt cgccttcatt cagtgcgtcg gctcccgcga
     2341 tgtttcctgc ggcaacagct ggtgctcgtc ggtctgctgt atgtacgcca ccaaagaggc
     2401 gatcatcggc aaggaacacg ccaaggggct ggagcccacg attttctaca tggacatccg
     2461 cgcccacggc aaggatttcg accggttcgt gaaccgcgcc aaggacgaat acggcatccg
     2521 ctacatccgc tccatgccgt ccgccatcaa ggaattgcag cagtccaaca acctgctgat
     2581 caaatacacc aaccccgacg gcagcttcac ggaagaagaa ttcgacatgg tggttctgtc
     2641 ggtcggtttg atgccgccca aagaagccaa aaagctttcc gccagcctgg gtatcgagct
     2701 ggaagagcac ggcttctgca agacggccct ggacaatccc gttcagacgt cgcgccccgg
     2761 cgtgtttgtc tgcggcgctt tcggcggccc gaaggacatt ccggaaaccg tcatggaagc
     2821 ctcggcggcg gcggcctgcg cggaggggct gctggcctcc aagcgcggca ccatgattac
     2881 gccggtggaa aatccggaag aaaaagatat gcgcggccag ggcgttcgca ccggcgtgtt
     2941 tgtctgccac tgcggcatca acatcggcgg cgtggtgaac gttcccgaag tccgcgacta
     3001 cgcggcgacg ctgccgacgg tcgtttatgc ggcggacaac ctctttacct gctcgcagga
     3061 cacggccgtg aagatggcgg aagtgatcaa ggaaaaggat ctgacccgcg tggtggtggc
     3121 ttcctgttcg ccccggacgc acgaagggct tttccaggaa aactgcgaga aagccgggct
     3181 gaatcgttat ttgttcgaga tggccaatat ccgcgaccag aactcctggg tgcatatgca
     3241 cgaaccggag aaagcaacgg agaaagccaa agatctgctg cgcatggcgg tcgccaaggc
     3301 ccagtatctg aagccgttga aacccggtca gttgtccgtc aaccatcagg cgttgattat
     3361 cggcggcgga ctggccggca tgacggcggc cctgtcgctg gccgatcagg ggtttgcttc
     3421 cacgatcgtt gaaaaggaag accgcctcgg cggcaattac aatcacctgt acaaaacact
     3481 ggaaggcctg gacgcccagg cgcacctgaa agggcttcat gaaaaagttt acaagaatcc
     3541 tttgatcacg gtcgtcaccg gcgccgccat tgaaaagatt gaagggttta tcggcaatta
     3601 caaaaccacc gtcaagtcca gggaaggcga gaaagttttc gaacacggcg tggtgctcgt
     3661 cgccaccggc gcgtatgaaa acaagcccaa agagtacctc tacggggaaa acgccaaagt
     3721 gaaaagccaa agagagctgg agaagatgat ctatgaaaag gatccgaaac tggcctctgt
     3781 gaaaaatatc gtcatgattc agtgcgtggg cagccgcgat aaggaaagac cgtattgcag
     3841 ccgctactgc tgcggagagg cgatcaaaaa cgcgctggaa ctcaaagccg ccgatccgtc
     3901 gcgtgacatt acgatccttt atcgcgatat ccgcacgttt gctttcaagg aagactatta
     3961 caagaaagcc cgcgaagcga atatcaaatt catctcctat gaagaaaacc gcaagccgga
     4021 agtggcggcc gccggagata aagtggaagt caaagtgctt gatccgattc tcaacgaaac
     4081 ggtcagcctg ccggccgatg tggtggcctt gagcatcggc gtggtgccga atccggaaaa
     4141 tgcggccatc ggtcagatgc tgaaaattcc cacgaaccag gacggcttct tcctggaagc
     4201 gcacgtcaag ctgcgcccgg tggatttcgc gaccgacggc gtgttcatgt gcggcatgtc
     4261 gcattcgccc aagtacagcg acgaaaccgt tacatcagcc aacgccgccg tttcacgcgc
     4321 ctgcctggtc ttgagccttg atttcatcga ggcggaaggc aaaacggctt atgtgaacaa
     4381 agaacgctgc tcggcctgcg gactttgcga aatcaactgc ccctacagcg cgattcgcgt
     4441 gaatcccgcc gaagggtgcg ccgaagtcaa cgcggttctg tgcaagggct gcggcgtttg
     4501 cacggcctcc tgccgcatga acgcggtgga tttgaacggt ttcaacaacg aagaagtgct
     4561 ggcgcagatt tataacatca acaattaagg cgaacggccg attcggccgc attgccgctg
     4621 gaaggagaat atatggctga aaacacagca accaaggaat gggaaccgaa attcgttgcc
     4681 attgtctgca actggtgcac gtacgcgggc gccgatctgg ccgggatcag cagaattcag
     4741 tacccgacca acgtgcgcat catccgcgtc ccctgcacgg gaagaatcaa tccgttctat
     4801 atcgtgaagg cattgcagac gggagccgac ggcgtcctgg tctcgggttg acacccgggt
     4861 gaatgccact atatctcagg aaatctggtg gcgcggcgca agtttgcgac gttaaaaagt
     4921 ttcctgaact acatcggtgt ggaaggcgac cggaccattt ttacctgggt ctccgcctcc
     4981 gaaggcgaca gatgggcgca ggtcatcaaa ggcgcaacgg agaaaatccg ggcgctggga
     5041 ccggccaata agatggtcaa gcagattggt tagtctgatt caggatcata atacataaac
     5101 ggggaaatca tcactgtgga aaacatcgaa acaaaattgc gggaagaagc aaaaaggctg
     5161 cttttggaaa aaaaggccga agtgattgtc ggctatgaaa aaggaacgct gccgctgacg
     5221 gcgacgcctt gtttcattac gaagccggaa gaggccgata agctggtctg gaacaacctc
     5281 tgcacgcaaa acctggccaa atttgtgcat gatctcattt cacagcacaa aaattcacag
     5341 aagcgcgtaa aaccggaaga ccggaaaaag aaagtcatcg gtgtggttgc ccgcggctgc
     5401 acgacgaggt cgctcattat tcaccttcag gaaagacagt atgaacgcgg cgacattttc
     5461 atcatcggcg ttccctgcga cggctacgtg gacggcaagg ggctgaaagt gaagatcggc
     5521 ggcgcggaca tcatcgacgg cgccgtcgag ggcggccaga tcaagatgaa aacgtccgac
     5581 ggcgacaaaa cgctggcgct caaagacgtt ctggcggact gctgtgtgac ctgccgcttc
     5641 aataatccca ttatttccga cgtcatgatc ggatcggcgg ccccgccgat gaatcccgac
     5701 gcggaatata atgaagtcaa ggaatttgaa gccaaggcga tggaagagcg ctgggaatat
     5761 ttcaccaaag aaatggccaa gtgcatgcgc tgcaacgcct gccgccaggc ctgtccgtcc
     5821 tgctattgtc caacctgctt cgtcgaacag agccagccgc agtgggtggg catcggcgaa
     5881 gatcaaacgg atacccaggt attccagttc atgcgtctgt accatatggt cggccgctgc
     5941 gtggactgcg gttcctgcgt ggccgtctgc ccgatgggcg tggatctgcg caagttcctc
     6001 aagaaactcg acaaggattg ctttgaattc ttcggcaacc gcgccggctc cggcatggaa
     6061 gacatgccgc ccctggggaa attcgatgag catcacgaca aaggcgattt tgtctacaac
     6121 ccgtaatagt ttgagatgat ggagtttaca tggaaacttt tctggaagaa gtaaacaaaa
     6181 agatacacgg tgttcccctg caggcctgct atcactgccg gaaatgcacg gccggctgcc
     6241 ccgccgcgtc ctacatggaa tttaacccca acaaggttat taaaatgatt cagaacgggc
     6301 agaaggacag agtgctgtcc agttcgacga tctggctgtg tctgtcctgc gaaacctgca
     6361 ttacccgctg ccccaatcag gtggacattg cgcggatgat ggatgtgctc cgccagatgg
     6421 ccattgaaga aggccgcgcc gcgaaggaag cgaatgtgct gaaattccat gaagcgttcc
     6481 tgtccagcat caagttcggc ggccgcgtgc atgaaatgct catgacggtc cattacaagc
     6541 tgaagtccgg cgatttgttc ggcgacgtgg acattgcccc cagtatgttt ttgaagggga
     6601 aattgtctct gttgccgccg cggacaaaag atttgaaatc ggttcgaaac atttttgaga
     6661 agacacagag caaataaagg aatgctgcgc ccccggccgc aaacggccgg atgcgctgaa
     6721 gtaaccagtg aaaaaggagg ctactctctt gaaagttacc tattatccgg gctgttcgct
     6781 gcatgggatg ggaaaagagt atgatcagtc catgaaggcc gtcagtcgcg ccctcaacat
     6841 cgaactcaaa gaagttgacg attggtcctg ctgcggagca tccgccgcgc acaacacaaa
     6901 ttttgatctt tccgttgcgc tgccggcgcg caatctgatc gcggccgaaa aggacgcgat
     6961 ggatgtgatg gtgccctgcg ccgcctgttt caaccgcttc aaaatggcgg agcatcacct
     7021 gaaggccgac aaagacctga aagcccgcat tgaaggcgta ctcggcgcaa aatatcaggg
     7081 cggcattgcc atccgcaatc cgattgacgt gatctacaat gaaatcggga tggatgccct
     7141 ggcggccaaa gtggtgaaac cgatgaccgg tttgaagccc gtttcctatt acggctgcct
     7201 gctgctgcgt ccgccggagg tctgccagtt tgaaaattac gaaaatccct ttatgctcga
     7261 caagatcatg aggacgatcg gcgcggatgt gaaaaactgg tcttacaaga ccgattgctg
     7321 cggcggttcg ctgtcggtca gcaaaacgcc catcgtcacc aacctgtgcg acaagttgat
     7381 ggcggcggca aaggaagccg gcgccaactg cgtggtcacg gcctgtccga tgtgcatggc
     7441 caacctggag atgcgcccca gtgcgaacat gaaaatgccg gtgttctatt ttacggaatt
     7501 gatttcgctg gcgctgggcc ttcccggttc gcaggagacc tttaaaacgc acctgacgga
     7561 tccgcagccg ctcttgaatc agctccggct ggtgtaatgg agtcgttgat aaaacccgac
     7621 gcttttgaag ctgaaaagtt agataaaata acacattagg gacaattatg gaaaaacgtt
     7681 tgatcaaaaa agacacactg gcaggcattg ccaagaaaat ggccggcgat ttcctggttt
     7741 gggcgcccgt gaaaaaagaa gacaatgttc tttttgaacc catggggaaa gacggcgaac
     7801 ctttctttgc gtatttgaat tccaaaaatg cgcccaagaa tgttttcttt ccgcatacgg
     7861 aaaccctgat gaaatatacc cggaccccca aaggcatggt gttttcttca gaagtcaacc
     7921 gggcggatca atccgttttg ttcggcgtgc gcccctgcga cgcgcacagc ttcgtccttc
     7981 tggataagct tttcgatcag gaaaaataca aggacgccta ctacattgaa aagcggaaaa
     8041 gcacgacggt gattgcgctg gcctgtgtga agccgcctta cgcaacctgc ttctgcacgt
     8101 cggtggacgg ggagccggtt tcggccgacg gcgcggacat tctgctgacc gacatcggcg
     8161 gcgattatct ggtggaattc atcaccaaaa agggcgaaaa gcttgcctcg tatttcggcg
     8221 atgcaaaggc cgatgcggcc gccgatgcca ggaaagcgga gattgccgcc caggccaaag
     8281 cggctgtgac gtcgaagatt ccggcgcacg aggtgaaacc gatcctggac gtcaattttg
     8341 acaatccctt ctggaacacg attcacggca agtgcctggc ctgcggcacc tgcacgtata
     8401 tgtgccccac ctgccactgc ttcgacatca gcgacgaggt gaaaggcgcc gacgggattc
     8461 gccttcgttc ctgggattcc tgcatgtttc cgctcttcac gaaggaaacg tccggacaca
     8521 atccgcgccc gtcgcagaag gaaagatggc gtcagcgcac gatgcacaag tttaaatatt
     8581 acgtggacat gttcggcgcg atttcctgcg tgggatgcgg acggtgcgtc atgtcttgtc
     8641 cggtgaatat tgatattcgt aaagtggttg aagatatttc aaaattataa tcagggagtt
     8701 cgtaatgcaa aacccgtata ttccttatcc cgttgtggtg gataaaatca ttactgaagt
     8761 cgataccaaa gacatcaaaa cgttccgctt caagtttgtc aacccggaag atgaacagaa
     8821 atatgcgtac attcccggtc agttcggcga actttccatt tacggcaaag gcgaatcacc
     8881 catcggcatc gcttcttctc cgacgcaaaa agggtacgtg gaatttaccg tacagaaagc
     8941 cggcgtggtg acctcggcgc tccatgaaat ggaagaaggc gaaatgatgg gcatccgcgg
     9001 cccgctgggc aactcctggc cgctgcagtt tctggaagga aaaaatattg tcattgtcgg
     9061 cggcggcttt gccttcacca ccctgcggtc tctgatcaat tatatgctgc atgaggataa
     9121 ccgcaaacgc ttcggcaata ttaccgtgat ttacggcgcg agaaatcccg gcctcctgct
     9181 gtataaggat gaattgacgg aatggggcaa aagaaccgac ctgaacctga acgttacggt
     9241 ggacaagggc gacgcgacct ggaagggcag agaaggattt gttcccgcgg tttgcaagga
     9301 agtcgctccc agcaaggaaa acgccgtggc ggtgatctgc ggtcctccgg tgatgatccg
     9361 cttcacgctg ccggtcttct ttgaactcgg cttttcgaag gaaaatatca ttacgtccct
     9421 ggaaatgcgg atgaaatgcg gaatcggcaa atgcggccgc tgcaacgtcg gcagcaagta
     9481 tgtctgcaag gacggcccgg tcttctctct ggcggagctg gataaactga cgccggaata
     9541 ctagccaccc ccgaattctt caaacctgtt ttaaaaggga gcctttgggt tccctttttt
     9601 ttgaagggac actccagcct gggcaagcgg agtcgggaga acaaaaaaat tgatgtttgt
     9661 ttttttctat gctagcagaa gcataaattg atccaatact cacgggagat gttgcatgcc
     9721 acaaacggtt accccttatc tggagatgtc ccaggcgatc atggaggcgg gcggcgaggc
     9781 gctgaaaaaa tgctaccagt gcggcacgtg ctccggaacc tgcccctgga cgccgatcac
     9841 gcatttcaat atccgcaagc tcgtgcgcta cggccagttg ggcctggacg gcatcgagga
     9901 tttcatgtgg ggctgctcca cctgccggtt ttgtgtggaa cgctgtcccc ggggcgttga
     9961 actcatcgac atcgtgacgg ccattcgcaa cgtgtacagc ggcggcggcc tgctgccgca
    10021 gagcctgcgc gcctttgtcg gctccctttc ggcccgcggc aatccctggt ccggcgaccc
    10081 ggccgtgcgc aatgactggg ccaagggcaa atatcccgtt tacaagcccg gcatggacta
    10141 cctgttctgg acctgctgca cggtctgtta cgacccccgc aacacaaggc tcgcgaaagc
    10201 ggcggcggag attctgaatc tggccggcgc aagctggggg atgccttctg ctgacgtcaa
    10261 ctgctgcggc gagagcctgc gcaaagtggg cgacgcggag cttttcggac ggctcaaggg
    10321 caaaaacctc gactacttca aatccaacga agtttccaaa atcattacgg tatccccgca
    10381 ctgtctggcg acgttcaaca acgactacgg ccaggactac gatgttacgc atttgagcga
    10441 actgatggct caatggatta aggacgggaa actcgttccc aaaaaagatt tcggcggcct
    10501 aaccgtgacg tatcacgatc cgtgctacct gggccggcac agcaaggtgt acgacgcgcc
    10561 ccgcgagatt ctcaaggccc ttccgggtgt ggagttcgtc gagatggagc gcaacagtga
    10621 agaaagcatg tgctgcgggg gcgggggcgg cggattgtgg acggagaaag ccaaaggcga
    10681 acgcctgagc gatttgcgca tcgaggaagc catggggacc ggcgctgcgg ttctggcgac
    10741 ggcctgtccg tactgcatca ccatgtttga agacagcgcg cggacgctca atgcggacgg
    10801 gcaggttcag atccgggatg tgacggagct cctcctggaa agcctggaaa tcaacatcga
    10861 cgaaccggtc gcggatacat gcgcccttta gataacgttg ccgccggctg gaaccgaacg
    10921 tatgacgggt tgagaacttt gcaccattac actttggtca tcgcgaggag cgaagcgacg
    10981 tggcgatctc ttcaaatatc aaatgctcat gagattgctt cacttcgttc gcaatgacag
    11041 agtagggcgc gatccctgat tgcgccgcaa tcctcgtcat accggcgaag gaggttgtgt
    11101 cagaatggtc aaaagctgtc attcccgcga agcctgccct cgagaagtcg gggggcggga
    11161 atccagagtc ctttaataat actggattcc tggtcgcgct acgcttgccc ggaatgacaa
    11221 aataggaatt accacacagt ctcgaaggcc ggtatccagg ttctgtttct tcgaaaagaa
    11281 gataaccggg accgggagtg aatgataaag caagagaaaa caacacgaag tggaatgatt
    11341 tgaatgattc aattattgag ctggatgccg gccttcgccg gtatgacaat atagggagat
    11401 agtatgagca gaattggtgt ttttgtttgc cactgcgggt tgaatatcgc gaagacagtg
    11461 cgggtgagtg agcttgccga gtttgcggcg actctgccgg acgtcgttgt ggcaaaagac
    11521 tacaagttca tgtgttccac ccccgggcag gaaatgatcg cgaacgacat cagggaacac
    11581 cggctggacc gcgtgatcgt gacggcctgt tcgccgctga tgcacgagca gacgttccgc
    11641 aaggtgctgg cggcctccgg tctcaaccag tacctgctga cgatcgtcaa catccgcgag
    11701 caggtgtcct gggtgacgca taatatcgac gaaggaacct ccaaggccaa ggcgctgctc
    11761 aacggcgccg tgaaccgctc ccgcttgctg atgccgctca cgtcgcgcgt gatcgacgtc
    11821 aaccccgatg tcatggtggt cggcgcgggc attgccggaa tccaggcgtc gctggaactg
    11881 gccaacacgg gcagaaaagt atatctcgtc gaaaagaatt caaccatcgg cggccacatg
    11941 gcgcagtttg acaagacgtt tcccacgctg gactgctccg cctgcattct cacgcccaag
    12001 atggacagcg tgctacagca taagaacatt cagcttttga catcctgtga agtgagcgag
    12061 gtcaagggct ttgtcggaaa cttcgatatt accatccgtc agaaggcgcg ttatgtcgat
    12121 cacgacaaat gcaacgcgtg tctcgcgtgc acggagaaat gccccggcaa gagcgtgtcc
    12181 gaatgggatg aaggcctggc gaagcgcaag gccatctaca ttcccttccc gcaggccgtg
    12241 ccgcagaagc cggtcatcga ccgggaggcc tgcaccttct tcaaaaaggg caaatgccgc
    12301 ctgtgcgaga aagtttgcga gcagaaagcc attgatttcg agcagcagga caccttcagc
    12361 accgtgaagg tcggcgcggt cattgtggcc acggggtacg acctgatgga tacccgggat
    12421 ttgattcagt acggttacgg caaatatccc ggcgtgtaca acgccctgga agtggagcgg
    12481 ctcttcaact ccgccggacc caccggcggc aaggtgatac tgcgcgacgg gaaagagccg
    12541 caggccatcg cgattcttca ctgcatcggc agccgcgaca agaacagcta cgagcattgc
    12601 agccgcgtgt gctgcatgta ttcgctgaaa ttcgcgcatt tgttcaagga aaaaacgtcc
    12661 gcggacgtct atcattttta cattgatatc cgcgccgccg gcaaaggcta tgaagaattt
    12721 tacaaccgca tcattgaaga ggacgtgaaa ttcattcgcg gcaagggcgc gcgcgtggcg
    12781 aaaagcgcgg acgaaccgga tcgtctgatg gtggaagcgg aagacacgat taccgggaag
    12841 ttcatccggc tgcccgtgga catggtcgtg ctctcgccgg ccatgattcc ccgttccaac
    12901 gccaaagaca ttgcgcggtt gtttaatcta agcacggaca agcagggctt tttcatggag
    12961 cgccatccga agctggcgcc gctgtccacg atgtcggaag gcgttttcat cgccggggtt
    13021 tgccagagcc cgaaggatat tcccgacacc gtggcgcagg ccaacggcgc ggcggcggaa
    13081 gccctgacca ttgtcgtcaa agacaagatg gagctcgaag ccacgacggt gatggtcaat
    13141 ccggcctcct gctgcgcctg ccagaactgc gtgcgcgtct gcccctacgg cgcgccgttc
    13201 ttcaaggagg acaaaggcgt atcggaaatc aatgaggcgc tgtgcaaggg ctgcgggctt
    13261 tgcgcgtccg tctgcccgac gggggcgatt atcgcaaggc attttacgaa tgatcaggtg
    13321 ctggcggaga tggaaggatt gatggaattc tagttatggc atcattaaaa ggccggatac
    13381 tgcgttgcac atcgtctcgc gtcactgcgg cgtatgaaaa tatacgcctc gttccacgat
    13441 actcgtgcgc cttgtctgcg gcgctttttc tgacgccata attattttaa aaggaaagga
    13501 aacgttcatg tcatttgaac cgaaaatact gggcattgcc tgcaactggt gcacctatac
    13561 cggggccgac cttgccggaa cgacgagact gcagtatccg cagaatctcc gggtcgtccg
    13621 cgtgatgtgt tccagccgga tcaacccgtc gttcatcttc cgcgcgttcc aactgggcgc
    13681 ggacggcgtg ctggtcgggg gatgccatcc gggcgattgc cattacaata cggggaacct
    13741 ctatgcgcga agacgcctgg ccgtgaccaa aaaaattctg gaatttgcag gcctggagcc
    13801 gggccggttc cgcgtggaat ggatttccgc ctccgaaggc aacaaatttg ccgaggtcgt
    13861 cacggagttc accggtcaga ttaaagcgct gggaccccag accaaattca agatttcata
    13921 gaagtacgag ggagcatatg gcagtcagct ttcagcaaat tgaagagaaa ttaaagaccg
    13981 aagcccgaag gcttttggaa tcgggccagg tgagcctggt gctcgcgtgg ggcagaggtt
    14041 ttgatgaaaa acatcccatg ccctatgttg ccaagggcgc ctccgatgtg gacggcatcg
    14101 tgttcaacga gttctgcacc cacaatctgg ccagatacct ggtccggtat ccgcggggaa
    14161 ccaaaatggc cgtcgccgtc aaggcggcgg acagccgcgc cgtggttcaa ctgattcagg
    14221 aagacaaggt caagcggcac gacctggtga tcctcggcat tcccgtttac ggcatcaaaa
    14281 atccgaagac gggggagatc atcgacggca agaccacctg cggcctgtac aaccccgtgc
    14341 tgtatgacat tctgattgcc gaagaggtgc acggacagcc ggtctcgtcg ccttacgccg
    14401 tgctggccga atacgaggcg atggatcagg acgcgcgctt cgcgttctgg aaaaaggaat
    14461 tggatcggtg catccgctgc tacgcctgcc gcaaggcctg cccgatgtgc tactgtgatc
    14521 cctgctttat cgatcagaac aaacccaaat ggggagaaaa gtctccggag acgccgggca
    14581 acctgatgta ccatctgacc cggtttcatc atctggccgg ccgttgcatc gattgcggcg
    14641 aatgttcgcg cgcctgtccg gtggacattc ccctgtatct gtttcacaag aaagtggcca
    14701 aagagtgcga agaaatgttc ggccaggccg cggggatgag catcgaagac aagcccgtgc
    14761 tggtcaattt ccgcatggag gattccgaca aaattttgga ataaggagct tttgttgtgc
    14821 tgaagaaaac cgccatccat caactgtccg atctgacttc cgcgctgatg ggaaagggaa
    14881 cgctggtcgc accggtccgg gaagacgccg gcttcaattt ccgggaaata tccgacgcga
    14941 agcaggtgga tctgaatttc cacaatacga tcatcagtcc caaatccgtc ttctttccac
    15001 agacggaaga tttcatccgc tatcggacgg gcagaccttt gcaggaggcc gaaacggtcg
    15061 agcccgaggg gaagcccttg attcttttcg gcgtccgccc ctgcgacgtg aagagctttg
    15121 agatcatgga catccatttt tcctgcaccg gcgccgtgga tccttactgg caggcccgaa
    15181 gagaagcggc caccctgatc ggatacgctt ttgatgttgc cgcgccggcc gagccggagg
    15241 aattttatca gacgctgggc atcggcgcgg ccgaccccga aggcagcgac atcttcatgg
    15301 tgcgccgcgg cgatgatctg atcctcaaaa gcatcaccgg caagggagag gcgctgctgg
    15361 ccggaatcga aggcctggcc ggggcgtcgg ccgatgacgg gaaatttttt gacgacacgg
    15421 tcgccgcagg gcgaaatttc aagacgcgtt ttacctgcgt ggacgccaaa gccattacga
    15481 agaaactgga agatcttttt cataacacgg atttctggga gaaggcgtcc aacgcctgtt
    15541 tgagctgcgg cgtgtgcacc tttgcgtgtc cgacctgcta ttgctttgat atttgcgatg
    15601 aaaccctgtt tgggcgggga acgcgccgcc gcgtgtggga cgcctgcatg ttcacggatt
    15661 tcacgctgga agccagcggc cacaatcccc gcacgaaggt gtatcagcgc ctgcggcaga
    15721 aaatcgggca caagtattcc taccatgtcc ggaaatacgg cgtcatttcc tgcgtgggct
    15781 gcgggcgctg cacgcggcac tgtccggtgg gcatcgatat tttttcgatc gtcgaagagg
    15841 ccatgaaggc atgatgccga acgctaaaaa gcacgttcgg gaataaacca atttgaggaa
    15901 agatatgcgt tcaacgaatc cctacatccc catgccggtg gaaattgtca aaatcaccga
    15961 tgaagtggat acccatgaca tcaagtcgtt ccgctttaaa tttctgaatg cccaggacga
    16021 ggcccggttt cagtatctcc ccggccagtt cgccgagttg tccatttacg gaaaaggaga
    16081 gtccccgatc ggcatcgctt cgtcgcccac ggagcccggc tatgtggcgt ttacggtcca
    16141 gcgggcgggc gccatcgttc ccgggctggt gacggctgcg ctgcacgata tggaagaggg
    16201 cggcagaatc ggcctgcgcg ggccgctggg gcatccctgg ccgattgaat ttctggagaa
    16261 aaagaacatc gttgtggtcg gcggcggatt tgcttttacc acgctgcgct cgctggtcac
    16321 ctatatgctg cacgagaaca accgttcccg cttcggcgac atcaccgtca tttacggcgc
    16381 gcggaccccg gggctgctct gctataagga tgaactggcc gcctgggaaa agcgaagcga
    16441 catcaaaacc atcattaccg ttgacaaggg taatgaaacc tggaagggcc gggaagggtt
    16501 tgtgcccgcc gtctgcaagg acgtcgcgcc gtcgcccgcc aatgccgtgg ccgtcatctg
    16561 cggcccgccc gtcatgatca aattcacgct gccggttttc tttgagctgg gtttttccaa
    16621 ggagaacatt ctgacgtcgc tggaaatgcg catgaagtgc ggcatcggca aatgcggccg
    16681 ctgcaatgtc ggctacaaat acgtctgttc ggatgggccg gtgttttcgc tggcggagct
    16741 ggaagagctg cctcgcgatt tctagagatg cccgtcgtaa agcaaatgac gagggtgagg
    16801 taaaaaatga agaaagtcat ggccatcctg acgatgatcg tttttgtcgc cggaccggcg
    16861 ctggccgcgg acaaagcgtt tcaaaaagcc gataaaaaca aagacggcaa aatcacaagc
    16921 caggagtacc tgaatgcgac ttccaaggcc tttgacgaac tggacaagaa tcgcgacggc
    16981 gttctgacga aagaagagat tcaggccaac gataagattg acgcggacaa gctgattcga
    17041 gaaacggacg caaaccggga cggcaaaatc ctgaaaaaag aataccagaa ggcggcaaaa
    17101 caaagatttc attccatgga taaaaacaag agcggccaca ttgactacaa cgaatgggcg
    17161 tccgaccgct ccgatcttta ctctcctttc aatctctttt cgttctgacc gtcgcgactg
    17221 ccctccgcgg gacgcaaatc ccggatttgg gacgggccgg aagccgtccg tccgaaagcg
    17281 gcgccggggg cttttcgcag agggtcgcgc attcttcaac acctttttat gattgatttt
    17341 ttgcatggat tgcattaacg tgcgccgact attttcaaag gaacagcaaa tgcgctatac
    17401 ggttcaaccg cttcagcagg gatacaccaa tcagatcctt tccatcaacg taacgaccgg
    17461 caagatggag gtccgtcctc ttcctcccga agttcgaaat ttctttatcg gcggaagggg
    17521 tctggggctt tatctgctgc atgagcaagt gacttcccgg accaaagcct ctgatccgga
    17581 aaatcccctg attttctcac cggggccgct gggcggcatt ccgcagtttc ccggaacgag
    17641 caagtgcatg gcgatttcgc tgtcgcccct gaccggcatt cccggtgtct ccaattttgg
    17701 aggccatttt ggcgcttatc tgaaatatgc cgggtttgac gcgctgacag tcgcgggaat
    17761 cgcccctgaa ccggtcatga tcgtcattga cggtttccgg cgggaaatct ccatcgtcgg
    17821 tgtttccgga gacgatcagg tttttcatct tgagaagacg ctggtggacc ggtttttgcg
    17881 cgagggccat gaaaaaaagg acctggcctt tttgacgacc ggcatcggag cggagcatac
    17941 gtctttcggt tgcatcaaca gccattacta tgacgccacg aagccggtgg aaggcggcaa
    18001 aggcctcttc cgcaccaaac aggccggccg cacgggcctg ggatccgtga tgagccaaaa
    18061 gcttgttacg gccattgttg ttctggccgc ttttccccgc ggtgaaaacc cttacggagc
    18121 ggccgactgg aacagagtca aagaggcggg ggcgaagctg cacggggttg tgcgggacgt
    18181 cgatccgcag gcgctgaaaa tgcatctcaa agggtcggcc ggtctgatct cttttatgaa
    18241 caaggaagaa taccagtccc tgccggtcca taattatcag cgcgggtcac accctcgggc
    18301 gggtgaaatc tgcggcaaag tttacgccga gcggctgttt gagcatcgcg ggatggacgg
    18361 gtgctttccg ggctgcaatc tgcgctgcac caagggcggc tgggtcatgc tgacgtcggg
    18421 cgagcatgcc gggcgaagag tctgggtgga cggccccgag tatgaaaccg ccgccggttt
    18481 cggctcgaat ctgggcatgt ggcatcccga atttgtcatg gaagccaact ggcactgcga
    18541 caattacggc atcgatacca ttaccacggc ggtcatcatg gcgtttgtca tggaatgctg
    18601 ccagcgcggc tacctgacga aggaagacac ggacggcttt tgtctgacgt ggggggatga
    18661 gcaggcggcg ctgcagttca tccatgacct ggcgtatcgc cggaccgact tggcccgcgc
    18721 cgccgccctg ggcatggtgg gcctgaccga ctgggtctct ctaaagtacg ctttgcgaac
    18781 cggcaaagca aagccgctcg aagagctgca gacgtttgcc atgcagacca aaggccttcc
    18841 tttctccctg tatcgcacgc accggtcgct ttccatgcag gcctcctacg cggcggccag
    18901 cgacatcggc gcgcatcatg ccgccgcctg gctggtcaag gtggatctgc tgggcgcctt
    18961 tcccacgttt gaatccaagg ccagagcgct gatcacctat ccccgtgtgc gcctgggcaa
    19021 cgacaatctg ggcctgtgca agctgccctg ggtggatgtt ttaaatcccg attccatgaa
    19081 ggaaaaccat accgacatct acatcaatcc ggcgtcgcag gagctttatg cggattttta
    19141 caacggcatg ctggggacgg aactgacctg ggaacagatc ttcgagcaga cggaccggga
    19201 catcaacctc cagcgtgtgc tgaatgtgct gtcgttcggg gcaacgaccg gggaacgcga
    19261 ctggattccc gaccgggcca tcgggcctac ggaggacacg ctctatgaac tggaaaaaga
    19321 atacaatgac cggcagctga gcgagattct gcagtggcct ctgccggaaa ttcagcagat
    19381 gcccacgggc gaaaagcgcg ccgcgctgat gcggcatcga aaagaggatc tgcgtcagtt
    19441 gattcttgtg tattaccgtg agcggggctg gacggatgcg ggtgtcccga aggtggagac
    19501 gctccagcaa atcggtttat ggcgttttct gacggacgaa gccagggcta tcattacccg
    19561 cctgaacggg taaagatttt cggccggaaa ggagtctccc gtgcttcaga atcaggtctg
    19621 gcagcctctt ccgggaatcc ggcaggcgga aatctatccg tatctgcgga agcccgatct
    19681 tctctcctcc aattcctgcg tcatccgcac cccccagcag attctgctga tcgatgccgg
    19741 ggcgctggcc gcgcagaccg cggatctgaa ccggatcctg gcggagtgtc agcgggaacg
    19801 cacccgaccg gttgtcgttt acctgaccca ctgccacctg gaccattccc tggaggtcgg
    19861 caggcaccgg cagatcatga cggcggcgcc cgtctggatt gccgtccagg aacagggggc
    19921 cgattatctg agccttgggg atccggcaaa aaccatcgcg gaactttacg gcgtggcttt
    19981 tccatccctg cggccggata tccgtctgct cacggaaaca gacatgagaa gaaaagcccc
    20041 ccgatcaatt tcactccagc cgggcgtgaa catgaggatc acgaccgagg ccatcgcaac
    20101 ggatctgaag gagcctctgt tccagcagac catctccatt ggcggaggcg atgtcctgga
    20161 actgtattcg gcgcccggac acagtcccga cagcgtctgc atccgcgtcg gcgaaattct
    20221 ctttatcggc gatttgctcg ccgcggccaa tccgatggtt gccggaattg ccggctggca
    20281 ccgggagcat ctcctcgaca cccagcggca tgtacaatgg cttttggatc atcaaccgat
    20341 cacgctctgt tatcccggac acggcggttt gattccgtcg gagaaagccc gcgacatcct
    20401 ggggcggctg cagcgcaaaa ccctctcgct gggcgacgtg agccggatga acgaggagcg
    20461 cctctttcag atcacggact acgcgctgga gctcatcgat gaggccgagg aagtattttc
    20521 atcgattgcc ggccggcttc tttatgtggc gtatcaactg gagcgcctgg aggaagaaga
    20581 ggcggcctgc cggtgccgcg acgcaatgcc catggagcgg attgacgcct gtctgctgga
    20641 atttcgcaaa ctctgccgga ggctggagtc cggaaaaatc cggcgggtgg aattcgcgca
    20701 tggcgccctg gcgattgtcg aacagatcaa agggctcttt gacccccgcc cgctgaccgc
    20761 cattctgccc cagccgatga ttcaccgggg cacaagcctg cttctcgatt ttatcggcat
    20821 cgccaacgga cgccgcaacc tggaggagtt tattccaacg gacgtcaacg cggtgatcga
    20881 cgatgtgctg cgggcgggtc gggagacccc tcatctggac gcctctgttc tggattatgt
    20941 ggaggatgag gcgcggtttc tggccgctct ggttctgcgc atcggccacg aaccggcagc
    21001 cgctcgtccc cccgtccatt tcctgccgca caagtccctt ccttacgtgt cggttgcacc
    21061 cggccgtttt tcggatacgc tcctgacgtt tctggactgg cttgcgcaga cgcacccgcc
    21121 gtccatccgc atcgcgaccg gcatgcatcg cggcggtccc tttgtgacga tagcgcccgc
    21181 gggctggggc gatgcggcgc cgacgcccca ccggaagaag aaaatcgatt cctttgcccg
    21241 ccggttccgc atgtgcggcc tggttctgca tgcgaaaaaa gaaggttttc ggctgaccct
    21301 tgccgaaggg ccggacgcgg cagacgtatc tgcgccggtt gatttacact gaataatttt
    21361 cgaaaaagtg gttgatttca gcgtcaaaaa aaattaagaa acaccctcaa gagcattgaa
    21421 tcccgctgtt gcgggacgag cgttcattct ccgcagtatg ctgcgatatt ttgcgttcat
    21481 ttcatgcctc aacagtgtgc tgcgaagtgg ttgatcaacg aaatatctga tacatttttt
    21541 ccggcaagat tcacccagta aacagtaatt tgaaaaattt ttttcgataa ggagaatgct
    21601 ttatggaaat caagaaaatt tgtgtgttgg gcgcgggact gatggggaac ggcattgcgc
    21661 agatttgcgc tcaggcgggg tacgaagtgg cgcttcggga catcgaacag cggtttgtgg
    21721 acggcggcat gaacacgatc aaaaagaacc tggcccggga cgtcgaaaaa gggaaaatga
    21781 cgcaggcgca tatggaagcg gtgctcggcc gcatcacgcc aacgctggat ttgaagcagg
    21841 cagccgccgg cgcggacgtg gtggtcgaag tcgtgatcga agtcatggac gtcaagaaaa
    21901 aggtctatgc cgaactggaa gccgtcgttc ccgaacactg cctgtttttt accaacacgt
    21961 ccgggctcag cattacggaa atggccgccg tgaccggccg tccggaccgt tttatcggca
    22021 cgcatttttt caatcccgtt ccggtgatgc gtcttctgga aattatccgc ggtcatcaga
    22081 cctccgatga aacgctgaaa accgcccagg agtggggcag gaaaatcggc aaggaggtca
    22141 ttacggtgaa ggaggccccg gcttttgtcg tcaaccggat tctgtgcacg atgctgaacg
    22201 aggccttctt tgtgctggac gaggggctgg cgtcggccga agacatcgat aaaggcatgg
    22261 tgctgggatg caaccatccg atcgggccgc tggcgctggc cgacctggtg ggcaacgaga
    22321 cattgctgcg cgtgatcgaa ggtttgcagc gggaactggg cgacaaatac cgtcccgcgc
    22381 cgattttgag aaaactggtc cgtgccggaa atttcggacg gaaaaccggc cggggcgttt
    22441 acgattacgg taaaaaatag catcatcctg caggcgggca caggcttccg ggccgccccg
    22501 gcagtctaat gacggaggga aaggagaaat ttcgcatgaa tttcgaattg acggaagaaa
    22561 tgagaatgct gaaggagatg gcctataaat ttgccgtggc ggaaatcgcg ccgttttccg
    22621 ccgaatgcga cgaacaggag aaatacctgc cggaaatccg gaagaaggcg gccgaaaacg
    22681 gcctggtcgg ggcgtggatt ccggaagcct acggcgggtc cggcgccggc attctgggta
    22741 atgccatcat tacggaggag ctgtccaaaa tcgatatggg catcggcctg aatatcgtcg
    22801 cttccggctt cggctgcgag tccattctca actacggaac ggaagagcag aagaagacct
    22861 atctgccgcc gatctgcgaa ggcgtccagg tttgcgccgg cgcctatacc gaacccaacg
    22921 cgggaacgga tgtctcgggt tacaagacgc gggccgtcaa ggatggaagc gattatgtga
    22981 tcaacggcaa caaaatgttc atcaccaacg gcaccgtgtg cgatttcatg gttgcgcaat
    23041 gcatcaccaa ccccgaacaa aagaagcaca acagttttag cctcatcatt gtgccggcgg
    23101 acgccagggg agtgacgcgc aacaaaatcc acgggaagat gggcattcgc gccagcgaca
    23161 cggcggagat cgcgtttgaa gacgtccgcg ttccccaatc caatctggtg gggaaggaag
    23221 ggaacggctt ccgccagctc atgcactttt tcgacatcac ccgcgtgatg gtttccgggc
    23281 aggccctggg gcttgcgcag gcgtgcctgg aaaccagcat caagtatgtc aaggaaagaa
    23341 ccgctttcgg cgcgccgctg ggctcttttc aaatcaccca gatgaagctc acggaaatgg
    23401 ccatccgcat tgaggcgctg cgcggcctgg tgtacaaggc ggcctggctg atcgatcagg
    23461 gaaagcccga ttacacgctc gcggccatgg ccaaattcta cggcggtcag accgccgtct
    23521 tctgcgcgaa ttatgccgtg gaactgcacg gcggctatgg atacatcgaa gagtaccccg
    23581 tgcagaaatg gtaccgggat gcgaaaatcc tggagcttta tgaagggacc aaggaagccg
    23641 aaatcatgac gatcggcagg gcgctgcaga cccggtaagc ttaccgggcc ttgccgctgg
    23701 tcctgtcctg atatttcttg acttcgtctg tgtcggaggg gggatagatg accacctttc
    23761 ccccctcttt tattttgatg ccgatgataa gtcccagcat ggagtaccac tcgccgattt
    23821 tccggccctg gttgtccaga atgtccaagc cgtgggggct ctgaagacag cattcattga
    23881 gccgcctttg catgttggtg acgaggtcgg ccatgatctc cgtcttcggc tcgatttttt
    23941 tccacaggtc ctcgtcgctg tcgatcacca cgtctttctg aaggccgaaa atggccacgg
    24001 gatacacgtc cgacccgctg ataaagtaat tgtaatcgct gcggacgatg aatttcttga
    24061 aattttccgt tgcttcgatg cttggcttga agctgcccat gtctttgagc agttttcccg
    24121 agcaaccggt aaacagcccc acaaacaccg cggccagcag ggccgctgaa atcatccccg
    24181 atttacgcat gtttacgctc cttggttgtg tcattttgtc ttgccgtttc cggcaggaca
    24241 tccggtttgg ccttcacgac cggtgttctg gctgtatcgg ccttgatccg cagccggcat
    24301 ttttgtaaca caaaacggga agtcatcaaa cataattttc aaaacctttt ttcaaaaaag
    24361 gggagcttct ttttttcgga ttgccgcggg cggctgtgga tccatgcgga atcaattcat
    24421 gattgacatc gcgggcggct tttcctatac tcaagacgcc catgagcaaa tcttccgcct
    24481 ctgacaaaac gctgttctcg aattgaccga taccgacagt ccgccgttct tccctgattg
    24541 cttccggcat ctcccccgct gaatgatcgg cgacggaaaa acatggacaa cctcaaataa
    24601 ggaaggcgtt tgatgaaaaa ttcccttgag ctcggcctga tcggcaactg ccgcatcggc
    24661 gctttgattg atgagcgcgg tgaaatcgtc tggagctgtc tgccccggtt cgacggagat
    24721 ccggttttct gctctctgct gcgcgaacat gccgacggcg aagggtccgg ctattgcgcg
    24781 gtggagctga tcgatcaggt ggaggccgcc cagttctatc tgcccaacac ggccatcctg
    24841 gtgacccgcc tgacggaccg gcacggcgcg tccatcgaaa tcacggattt tgccccccgt
    24901 tttcatcagt acggccggac gttcatgccg atgatgatga ttcgccagat ccggcgtatt
    24961 cacggaacgc cgcgcatttg cgtgcgcgtt cgccccgtgt gcgaatacgg aggccagagc
    25021 tgcaccgtca cccacggcag tcatcacatc cgctacgtcg caccgtcctg gatcttacgc
    25081 ctgaccaccg acatctccgt gaccgccgtg cttcaggaaa tccccttttt cctggaaacc
    25141 gccgccacga tgattctggg gccggatgaa accattccgg acgccatcgg cgatctttca
    25201 cgccgttttc tggacgaaac ggtcggcagc tggcgcaact gggtcagaga cctttccatt
    25261 cccttcgagt ggcaggaaga ggtgattcgc gccgccatta ccctgaagct gaacacgttt
    25321 caggatacgg gcgccatcat cgcggccatg acgacatcga ttccggaggc gccggattcg
    25381 ggccgcaact gggactatcg ctactgctgg ctgagagatt cctactttgt cgtcaacgcc
    25441 ctgaaccgcc tcagcacgac caatacgatg gaacggtatt tgaattacct gatcaatgtg
    25501 gcggcgggaa cgcccaacgg ccggattcag cccgtgtacg gcattgacgg cagggccaag
    25561 ctggacgagc gggacgtcga tagccttccc ggttatcggg gaatggggcc cgtccggatc
    25621 ggcaaccagg cctgcgaaca gatccagcat gatgtttacg gatcggccat cctcgccgtg
    25681 gcgcacacct tctttgaccg cagacttgcc caccggggag gagcggctct cttctcgcgg
    25741 ctggaacccc tgggggaagc agccatggcg gttcatgacc agccggattc gggtccctgg
    25801 gaaacacgca acaccctgcg ggttcacacg ttttccagcc tgatgtgctg ggcggcctgc
    25861 gaccgtctgg cccgcatcgc cgtttatatc ggcctttccg aacgcgctgc ctgctggcaa
    25921 agccgggcgg aacggattca tgccaccatc tgccgccgtg cgtggaatga aaaaaagcag
    25981 tctttcgtcg gcgcatttga cggagattcg ctggatgcca gccttctgct gctccacgac
    26041 atcgggtttc tgtccgacga tgatcctcgt tttgtccgga cggtggccgc ggttgaacgg
    26101 gagctccggc gcggcgatta tatttaccgc tatgtggaga cggatgattt cggcgttccc
    26161 caaaacgctt ttgtggtctg caccttctgg tatatttacg cgctggctgc cctgaaccgc
    26221 ggcgaagaag cccgcgcgct gtttgaaaac ctcctgtccc gccgaagccg ccacgggctt
    26281 ctggccgagc acatcgatcc gcgcacggga gatccctggg ggaattttgt tcagacctac
    26341 agcatggtgg gcctgatcaa ctccgccatc cgcctcagca ggcgctggga cagtgcgttt
    26401 tagaagaggg gggcggatga gcctgcaaag catcaaatct tcccgatcgc ttcgccttac
    26461 gctgcgcttt gtcattcccc tcgtgctggc catggcgctc ctggctttcg cggtgttgcc
    26521 tctggtggac aagctgacgc tgcactggtc ggtaagggat atggatatcc ggtcaaagct
    26581 ggtgaccagc accatccagg accccctggc cgaacttctg gaacagggca acaaaagaaa
    26641 aattcaatcc ctgctttccc gcgccacgca ggatgagcgt cttttcgcat tgggctattg
    26701 cgatattcag ggaaagctgc tcttcaaaac acgggatttc cccgacatcc tcggctgttc
    26761 tgttccggat ccgtccgtcc ggaaagcgtc ttcgctgctg gaattgccgc aggggctggt
    26821 gcatgttgcc gtcaatccct ttttgattga gggactgccg atgggttccc tgatggtgct
    26881 tcacgacatg agctttgtca ggcagcgaag cgaggatacg cgaaaatacc ttctggttct
    26941 gtttgccgtt ctttctttgg tgacctccgt gattacggtg ctgatcgccc atttgagctg
    27001 gcgcggctgg atgaccggtg ttcgcgccct gctgcgcggc gaaggcctga tcaaaccttt
    27061 tgccaaatct tccagctccg agcttcagcc gctggtgggc gatctgcgcg cccttctgcg
    27121 cacgatggat gcggaaaagc gcatagccga tgatctcacc atttcctgga atccggattc
    27181 gctccgcagc ctgctcttta aacaactggc cggggagcag attctcgtcg tttccaaccg
    27241 ggaaccttac attcacatga aaggaaaaga agggatggag gttcagcgcc cggccagcgg
    27301 actggtgacg gcggtggaac cggtcatgcg cgcctgttcg gggatatgga ttgcccacgg
    27361 cagcggcaca gcggaccggg aggtggtgga caaaaaggat catgtccgcg tgccgccgga
    27421 acatccggaa tacacgctgc ggcgcatctg gcttacggaa gaagaggaac ggggctacta
    27481 ctacggtttg gccaacgagg gcctctggcc gctgtgccac atcgcgcatg tgcgccccgt
    27541 cttccggacc gccgactgga accggtacgt ggcgatcaat cagcggtttg ccgatgcggt
    27601 ggtccgggaa gccgaacggg aggatccggt cgttctggtt caggactacc attttgccct
    27661 gctgccgggt atgctccaac aggtccttcc caaagcaacg atcatcacct tctggcacat
    27721 cccctggccc aatccggaat ccttcggcat ttgcccgtgg cgggaagaaa ttctgcgagg
    27781 catgctggga agcacgattc tcggtttcca cacacctttc cactgcaaga attttctgga
    27841 aaccgtggac cgctatctgg aaacgcgggt gaattatgct tcttccacga tttctcacgg
    27901 cggcaatctg acgctggtcg aagcgtatcc gatttccatc caatggccgt cgccgtggca
    27961 ggatacgcag ccgccggttg cccgctgcag ggaagacatc cggcaggagc tgaacctcgg
    28021 gcgggatatc ttggtggggc tcggtgttga ccggctggat tacaccaagg ggattcttga
    28081 gcgtttgagg gccgtcgaat gcctgctgga gacgcatccg gacttggtcg gccggttttg
    28141 tctcattcag atcgccgctc catcgcgatc ggttcttgaa gaatacaagg cctttgagtc
    28201 ccgcgttcgg gctttggccg aacggatcaa cgcgaaattt tcccgaaacg gctatcagcc
    28261 ggttcatctg aaagtggagc atcacgaacc gcggaacatc aaccgctact accgcgccgt
    28321 ggatgtctgc ctggtaacga gcctgcatga cggcatgaat ctggtggcca aggaattcgt
    28381 ggcggcccgc gacgacgaac ggggagtcct cgtgctcagc cgttttaccg gagcggccca
    28441 cgagctgtat gaagccctga tcgtcaaccc ctatcacatc gaacagacgg ccgacgccct
    28501 ttatcaggcc ctcaccatgc ccgaattcga acaacaggcg cggatgcgca gcatgcggtc
    28561 catggtgaga gatttcaacg tttaccggtg ggcggcccgg atgcttctgg atgcggcgcg
    28621 catccgccgg cgcgagaagc tggcggaacg gattggtgtc gcgctatgag gctggacctg
    28681 cccgctgttt atctcttcgg aaggaccgcc tgctccgtcc tcgatcgttt tctggatcgg
    28741 tcaacactct ttgcctttga tctcgacggc acgctggcgc ccatcgtatc ggatccggac
    28801 gccgccggaa tctccggggc tgttcgggag gaattatccg ttctagccgg gatggcgccc
    28861 gttgcgatca ttaccgggcg ttcccgcccg gatgccctgc ggcatctggc gtttgcgcct
    28921 cgttatctgg tcgggaatca cggcgcggaa gggctttcgg gatggaaggc gcgggaaaaa
    28981 acttttgtcc gcacggcgca gggctggcag tgtcagctcg atacactcat tccggtggag
    29041 gaacggagag gcatcgtggt ggaaaacaag ggctcaacga tttccatcca ctaccggcac
    29101 gccgaaaacc ggaataaagc acatgccata attcgacggg ccgccggccg gctggttccg
    29161 ccgcccagac ttgtcagggg caagtgcgtc gaaaatttaa ttcccgaagg cgctccggac
    29221 aaaggcgctg ctctgaagct gctcatgcgg caggcggggt gtgcaaaggc gttttttgcc
    29281 ggagatgacg agacggacga agatgttttc cgtctgaacc atcaacgcat tctcgccgtc
    29341 cgtgtgggcc gcaagaatga cagcggggcc cgtttctttc tgcgggatca atctgaaatc
    29401 gttcgtcttt tgcgcgagat caaccggatt ctcgggcgga cctgatcccg gctgtttgat
    29461 ggccgttggt catgttttga tgacttcccg ggcgatgacc acgcgctgaa tttccgatgt
    29521 gccttcatag atggttgtgg cgcgggcatc ccggaacagg cgttcgacct tgtactcttt
    29581 ggtgtagccg tagccgccgt ggacctgcag cgccatgtag gcggccttgt tggccgtttc
    29641 cgaggcgaac agtttggcca tggaagcctc tttggtgaac ttcagtccgc gatccttgcg
    29701 gtcggccgcg gccagcgtca gccaggttgc cgcttcaatc cgggttgcgg catcggcgat
    29761 catgaattga atggcctgga acgagttgat ggtttttccg aactggcgcc gctgctcagt
    29821 gtaggaaagc gcttcatcca gagccgccct ggcaatgccg acggcctggg aggcgatgcc
    29881 gatccttccg ctgtccaggg cgcgcatggc caccttgaat ccctcgccgg gattgcccag
    29941 caggttcttt gcggggacct cgcagtcttc gaacaccagt tcgacggtat tggaggccct
    30001 cagccccagc ttgtcttctt tggttccgat tttaaaaccg ggcattcctt tttcgatgat
    30061 gaacgcagag aggccttttg ttcctctttc ctggcccgtc cgggcgatca ggttgacgac
    30121 atcggcatac tggccgtgcg tgataaagac tttggtgccg ttgatgatgt atttgtcttt
    30181 ccgaaggacg gcggtggtcg tcatcgaacc cgggtctgag ccggcgcccg gctccgtcag
    30241 agcaaacgcg ccgaggagcg atccttccgc aagtcccatc agccattttt ctttctgctc
    30301 cgggctgccg aacctgagaa gcggctccgt ggagagattg gcgaccgaca tggtgactgc
    30361 gctcgacgcg cacgcatagg cgatttcctg cagcgccaga gaataggcca ccgtgcccgc
    30421 gccggagccg ccgagttcag gcggcaccat catgcccaaa aggccgagcc cccccatttt
    30481 tttgaccgcg tccatcggga aaatgccctg acgctcccat tcggcggcca tcggttccag
    30541 ctcttttctg gcaaaatctc tggccatgag ccggatcatt tcctgttcgc ttgtaatttc
    30601 aaatgccatg gttggttcct tcagggaata tacagaacga cgagaagttt cgttttatcg
    30661 gaggaaggat tgctcagcgc gtgatgcagc gccgaattga agtgaatgca ttcgcccgga
    30721 ctcaacagac tggtgttttt gccgaccgaa atggaaagct ttccctttaa gacatagacg
    30781 aactcttccc cttcatggtg gtattccacg ccttcgtggg cggtgttggg ttctatggtg
    30841 acgagatacg cgcccaggtg cttatcggcg ccggatttgg tcaactgcgt ataggcgtag
    30901 gaaccggcgc gctttttctg gcttttgact ctggaggatg cttttttgtt ttcgtcgccg
    30961 ttaagctggg cgacatccac tttcagcgtg cggctcaact gcagaacgag agccaccggg
    31021 gcggtggttt catccttttc aaccttttcc agcaggtcgg ccggatagcc tgtttcatgc
    31081 gccaggtcct taatgctcag ctttctgctt tcgcggtacg ccttgatgct ctgaccgaag
    31141 gacttcttgt gttctttgac ttttgccatg ggcttcatcc tgtaaaaaag agggtcactt
    31201 gatcttctgc agcatttctc ccatcatttt gtgaaccagg ttaatggctt tcagcggacg
    31261 gatcatcacc ttgaaatcgg tgattctgcc gtcttcaccc caggtgatca tatctacgcc
    31321 gttgatggag atgccgtcga tcaccgtcag aaattcgagc acggcgttgt tgccggaaat
    31381 gacttcccgc agatattgaa acgtgtcgtt gttgaggacg tgcagggccg ctgtcaggta
    31441 cagcgtggta atcggctttc cggcttgcgg cgtatggacc acgggggaat gaaagacgac
    31501 attttccgcg agaatgccat ccaggcccgc tgcgtctctt tctttgacca gtttgtgcca
    31561 ggtggcgatg gtgttttcaa tcatggttgc acctctttct tattggccgg gaacgatgag
    31621 cttggaggct tcctgccgct ccatctcctg cgcttcggcg atcatgccct ttaccgcttt
    31681 ttcaatggcg tcgggcagct tggctgccgc ttcagggagc gttttggcgc cttcaatcag
    31741 acactgaacc gggatgggtc cgttggggga catgagttga gccatcccgg tgaaaagggg
    31801 ttcccggctc tcatcaggcg agccgtcgat gttgaccggc gaaagacgcc ggatggtggc
    31861 gaaggtcagg tccgtgaaag actcttcttt gtaaagattg tcgggattga tcttaatgtt
    31921 tgtgattctg ggctcgtccg cttcgctcat gatggtctcc ttttaagatg gggtgatggc
    31981 tcatgccccg aaaaaaataa aaggaaacgg tgggcaaaac ggcctccttt ctttcggatc
    32041 attgaccgct ttcctacact gaaaaagcat gggggtcaag gagggatgtg agggaatgat
    32101 ggatggtgaa aaacaggcaa gaggcattag gcgatgggcg atagggaaaa gctcatggct
    32161 catagtaaaa agcgacgagc gatgtgtagg gaacggtttt aaaccgtttt ttgcaggcgc
    32221 agatcatggg tgaatgtagt agggaacggt cgcgaccgtt cccta
//
LOCUS       DAOSRM010000012        29958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1701_length_29958_cov_98.695950, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000012 DAOSRM010000000
VERSION     DAOSRM010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 29958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29958
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1701_length_29958_cov_98.695950"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLA58_02470"
     CDS             complement(<1..926)
                     /locus_tag="PLA58_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HOR61689.1"
                     /translation="MARKEIMQKVAGYDEIIRDNTILEKYGFDYPARKGEVQKIIDSL
                     HPQRLDLIVSEIREQTASAKSFRLVSGNGALPPFQSGQYINVFLEAGGIRTSRPYSIA
                     SSPTQTGYYEITVRRVEDGFVSNVLLDELKPGDRLQASSPAGNFHYNPLFHGNRLAFI
                     AGGSGITPFMSMIREMADRNLDRRLHLFYGSRVEEDVIYRQEIERIAEAHKNFTWDLV
                     VSEPDPSFSGCRGFIDANLIGKKLEGTDWTFFVCGPEAMYQFCLPQLEKLSIPARKTK
                     VEVMGAPKNIVSYPGWPENVKAGDVFQVAVKG"
     assembly_gap    1069..1168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1169..2292)
                     /locus_tag="PLA58_02475"
     CDS             complement(<1169..2292)
                     /locus_tag="PLA58_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR61690.1"
                     /translation="MSDYDVVVIGAGNGGLTAAAGLAQKGHKTLLLERHNVPGGCATS
                     FVRGRFEFEIALHQLSGMGLPEFPGPLRGLLGELGVLDKIDFIQQQNLYRVAIPGRMD
                     INLKADRQAVIETLIEKFPAEKEGIQKFFDLLYAFCNELVNVLFIHDPEASPEKYPLF
                     FKLALQNSQGILDQYLKNPLLQTVVTIYWSYAGLPPSRLSFMELAMVIWAYIEFKPWH
                     MKGGSQAMSNAICDAFLRFGGDTRFNCAAKKIRVQNGKVVSVVTEDGDEISTKFVVSN
                     AGPIRTLIDMVGAENCPEDELKKLNARTVGLSAFTLYMGFDCDPSEMGIDKATNFICG
                     TADADYAHSKMKTLDGAEYSLLTCYDVDDPDFSPRGACQVS"
     gene            complement(2487..3395)
                     /locus_tag="PLA58_02480"
     CDS             complement(2487..3395)
                     /locus_tag="PLA58_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOR61691.1"
                     /translation="MMKAPLVFGIGQCSLDHLGLVAAYPPPDVKCEFTNLLLQGGGPA
                     ATALVALKRWGARCHIAGVLGDDGFGTQIKALLDAEGLDTSGLQIRKQHQSQFAFIAS
                     EPALARRTIFYQMPTGEPLRPEEINTGILLSSQALHTDGLFTEASLFACRKAKEAGIP
                     VIVDAGTLRDGMLEMAKMSDCFVTSEVFSNAFAETPAETCRKLAGMGVRLAGVTLGKR
                     GYVALVDGCFIEKEAYPAKAIDTTGCGDVFHAGLIHGILHGWSPEKCLDLGAWAAARV
                     SEQMGGRSGIPDIHTLQLKYSDEMNR"
     gene            3750..4373
                     /locus_tag="PLA58_02485"
     CDS             3750..4373
                     /locus_tag="PLA58_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOR61692.1"
                     /translation="MKVLLINGSPNKKGCTYTALCEAEKELNKQKIETEILYLGQKPI
                     RGCTACGKCLETGKCVFDDTVNVALEKAAQSDGFVFGSPVHYAGASGFITSFLDRCFF
                     AGSDVFAYKPGAAIVSCRRGGATAALDQLNKYFTINNMPVVSSQYWNMVHGNTPQEVL
                     QDLEGMQTMRGLGRNMAWLLKSIQAGKKSGVALPAPEERIWTNFIRS"
     gene            4484..4873
                     /locus_tag="PLA58_02490"
     CDS             4484..4873
                     /locus_tag="PLA58_02490"
                     /inference="COORDINATES: protein motif:HMM:NF037404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOR61693.1"
                     /translation="MSDEREIIKYWSTTSKQDFETAEILFENQKYSHALFFCHLSIEK
                     ILKALIVRKTHAAPPLVHDLVRLAEKTGISLSDKLKGELAEISSFNIQARYDDYKLSF
                     YKKAKKRFAAKYIAATREILIWLRAQL"
     gene            4852..5184
                     /locus_tag="PLA58_02495"
     CDS             4852..5184
                     /locus_tag="PLA58_02495"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOR61694.1"
                     /translation="MAKSATLKKKETDLINRYVLLLRQKGIDVSKVVLFGSYAKGSAK
                     PESDIDLAIVSSQFGKDHWKEMVLLRKLALEIDSHIEPLPFAPEDVEDRYSALSQEIK
                     KTGILLKI"
     regulatory      5233..5333
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5393..>5789
                     /locus_tag="PLA58_02500"
     CDS             5393..>5789
                     /locus_tag="PLA58_02500"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOR61695.1"
                     /translation="MAITAAEIFKSVEDIRAERPLIHNITNYVVMNNTANALLAIGAS
                     PVMIHAEEEVEDMAAVAGALVINIGTISAPWVRGMFKAFAKAASRGVPVVIDPVGAGA
                     TPYRTETVRDLIRSGRPTIIRGNASEILAL"
     assembly_gap    5790..5889
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5890..6351
                     /locus_tag="PLA58_02505"
     CDS             <5890..6351
                     /locus_tag="PLA58_02505"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOR61696.1"
                     /translation="RSGRPTIIRGNASEILALRDDRLKTKGVDSTAASSEALYAAQSI
                     SKTYRCAVCVSGAVDYIVEGKRLVKVANGHPMMPKVTGLGCTASALCGAFAAVDRDPL
                     TAAAKAMAVMGIAGEIAAEQSPGPGSLQMHLIDALYRLTKEDMERRLKVEG"
     gene            6354..6995
                     /gene="thiE"
                     /locus_tag="PLA58_02510"
     CDS             6354..6995
                     /gene="thiE"
                     /locus_tag="PLA58_02510"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOR61697.1"
                     /translation="MRGIYLVTDRGLCRGRLLLDVVAQAVKGGAACVQLREKEMPTRA
                     FVEEALAVKNILAPHRIPLMINDRIDVALACGADGVHIGQEDMPYETARRLLGPKAII
                     GLSVETWADVEASRDLDVDYLGVSPVFATPTKTDTKEPWGLEGLRKIKAFSRHPLVAI
                     GGISEANACEVTEAGANCLAVVSAICSADDPAGATARLKHVFDEALRRRGNRD"
     gene            7270..8280
                     /gene="aroF"
                     /locus_tag="PLA58_02515"
     CDS             7270..8280
                     /gene="aroF"
                     /locus_tag="PLA58_02515"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOR61698.1"
                     /translation="MIIVMKSRATEKQIENVIAWIESVGYKAHPSSGVERTIIGAVGD
                     QRDKAALKYAENLPGVEKTMPILKPYKLASREFHEEDSVVRVGAAEIGGPEFVVMAGP
                     CAIESEEQLLESAYVVKKGGGQILRGGAFKPRTSPYSFQGMEEEGLKVLESVSGKTGL
                     PTVTEVVNPADVDLVESYADMLQIGARNVQNFALLKKVGHARKPVLLKRGMMTTIEEL
                     LMSAEYILSSGNPNVVLCERGIRTFETATRNTLDISAVPVLKSLTHLPVVIDPSHAAG
                     NWKYVIPLSRAALAVGADGIIVEVHPEPEKAVSDGAQSLKPEKFYMLMDDVRALAKAM
                     RG"
     gene            8298..9392
                     /gene="aroB"
                     /locus_tag="PLA58_02520"
     CDS             8298..9392
                     /gene="aroB"
                     /locus_tag="PLA58_02520"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOR61699.1"
                     /translation="MKMLKVHLDKKALSSHEIRIGRNILDRVVLLLAKNHKASRYGIV
                     TDTHVNRLFGKTLLESMSGAGLNTFLLEIPAGESSKTIATAMDLAGRLLEAGADRGTL
                     LIALGGGVVGDLTGFVASVYMRSVPYVQIPTTLVGQVDSAIGGKTAVDLPQGKNLLGT
                     FYQPRAVFADLRFLDTLPEREFVGGLAEIIKYGIIDDEKLFQRLEEDMEAVKRRDPER
                     LLTLVETCCRIKKSIVEMDEREQGLRRILNFGHTLGHALETASGYALSHGEGVALGMI
                     AAARLSVQMKYLPQSEAKRVEKVIAGAGLPVRIAATLDTKAVMDSLKMDKKKRNNVVH
                     FVLIRKIGMPFVNGSIQDETILSVLEGMKA"
     gene            9389..10468
                     /gene="pheA"
                     /locus_tag="PLA58_02525"
     CDS             9389..10468
                     /gene="pheA"
                     /locus_tag="PLA58_02525"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOR61700.1"
                     /translation="MKQPLETLREEMRKKDRDIVRLLNERSEISVKIGRVKGQAGFPV
                     YDPAQEARVLRYLSELNGGPLSDSQVTSIFREILSASRDLQKPTRIAYLGPEASFSHQ
                     ASLLHFGASCQFFPQQGIARVFDEVEKGEVEWGVVPVENSLEGSVNVTLDRLALTPLT
                     VQAEIDLRISQCLISQAKSLKGIKRVYSHPQGLAQCQSWLRAHLPNAATGEMESTAAA
                     VAMVRGKKTAAAIGSALAASTYGLNLLARGIEDNSSNTTRFLVIGRGDTFPTGDDKTS
                     IIFATPDVPGSLHRALSCFAKRRINLTKIESHPVKERLWEYFFFVDMIGHLRDRSAQN
                     CLAELKDKTIYLKTLGAYPRAKDGR"
     gene            10465..12024
                     /locus_tag="PLA58_02530"
     CDS             10465..12024
                     /locus_tag="PLA58_02530"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOR61701.1"
                     /translation="MICIPVMDPTHREALRSAQKSASLADLIELRMDWIADGDVSELI
                     RAARRASASVKIIVTCRRREESELAREGLEALSVKERTNAAKMKLLREAVVSGADFID
                     IELAEGTRAVSRMRSWCHSRPNPARLIVSWHDPYKTPSLASLKKIFEACARAGADIVK
                     IVSYARSLSDNAAVLNLIDYAKHQGREIIAMCMGEKGQISRVLAPSRGSYLGFAVLPG
                     GRASAPGQLTLREMRTFQKLLRPHRPSIEPRQNQLHFVLLGNPVRQSLSPLMHQAAFR
                     TMGIDASYSAFCIADPAGAVSGIRAMNIRGASVTIPFKTAVMEYLDDIDADAAAVGAV
                     NTIVNDCGRLIGHNTDWLGLMAALKEKTQIRGRRFVILGAGGTARAAAYGIRKEGGRP
                     VIVNRTPEKGMALAMQFGCPFYPLAQISKIKAFGLINTTPVGMFPDIQKSPVPPSVLA
                     NYRVVADVVYNPLKTKLLRDAEAKGCKVIPGLEMFVRQGAEQLMLWTGRAAPLAVMRN
                     AVRERLMQHES"
     gene            12014..13270
                     /gene="aroA"
                     /locus_tag="PLA58_02535"
     CDS             12014..13270
                     /gene="aroA"
                     /locus_tag="PLA58_02535"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOR61702.1"
                     /translation="MNPEKDLPGKVSSVEVPGSKSLTQRALIAAALARGESLIRGALI
                     AEDTRHLMEGLNLLGARLEGVDGGIRVVGTGGAIANPDRAIFLGNNGTALRFLTALVC
                     LGRGTYVLTGEPRLLERPVGPLVEALKGMGVNIACKNQCPPVTVSANGLRGGQITLTD
                     IESSQYVSALLLCAPYTAKGIELSLTGNIVSAPYIDLTVGVMKDFGAKIYRTGRDLFS
                     VGTQSIYEGRAYDVEGDVSSASYFFLAAALLKRPVRVYGIRRESAQGDIGLLDVLEKL
                     GCRIESGEAWVEVSAPNSLAAGDVTLDMGNMPDMVPTVGVLAAYRRGKTVMTNAAHLK
                     IKESNRLAAMASELSRVGIAASERPDGLTIEGGIPHAADIETYNDHRIAMSFAVAGLA
                     TPGIEIADKKCVDKSFPGFWRELDKL"
     gene            13267..13830
                     /gene="aroL"
                     /locus_tag="PLA58_02540"
     CDS             13267..13830
                     /gene="aroL"
                     /locus_tag="PLA58_02540"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HOR61703.1"
                     /translation="MKIILIGCRASGKSTVGRLLSRKLGVPFVDADQLVEKTAGMSIA
                     EMVALKGWQEFRRRETQTLASLQDPSVCVLATGGGVVLADENRRLLKKTGVVIYLKAD
                     LPDVVERLRRDAKNTQSRPPLTDGDLIEETRSVLLQRAPLYRSVADYTVETRNKNAEQ
                     AAEEIYRQLLEAGIMSKMQTLKQKTIE"
     gene            13848..14924
                     /gene="aroC"
                     /locus_tag="PLA58_02545"
     CDS             13848..14924
                     /gene="aroC"
                     /locus_tag="PLA58_02545"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOR61704.1"
                     /translation="MAGNRWGTLFCVTTWGESHGPALGAVIDGCPAGIRLTPEEIQRE
                     LDRRRPSTSAASTSRAEKDRVEILSGVFEGQTTGTPIALLVRNTDARSEHYDELRNVF
                     RPGHGDYTYWKKYGLRDWRGGGRASGRETVGRVAAGAVARKVVRAAGMEVIAYTRSIG
                     GIGISPDALTRDQHWEKRVRESPLYCPDPQAASAMEERLAAVRKEGDTLGGVVEILVR
                     GCPAGLGEPVFDKMDADLAKALMSIGTVKAVEVGEGCAVASKNGSEINDPMDAAGFVK
                     NSAGGILAGITTGQDLVCRVYCKPIPSIALPQQTVDESGRERSIRVGGRHDICVIPRI
                     VPVCEAMVLLVLADHWLRQKAGGR"
     gene            14921..15694
                     /locus_tag="PLA58_02550"
     CDS             14921..15694
                     /locus_tag="PLA58_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOR61705.1"
                     /translation="MKKMTAGIIGGTHGMGRWMAGLLRAQGLAVSVAGRATKTTAVDL
                     AGRCDVVVVAVPIAATAGVIREVGPRVKPGRLLMDLTSLKTEPVALMLSHSEADVVGC
                     HPLFGPSVSDPAGHSVVLCRGRGENGYALMKAVFEKAGYRVLETTPRRHDKMMSVVQA
                     LNHFNTIVLGMAVAEAGIPFPELQPFSTPIFTTKMEIVRKVFTDTPGLYADILAGNPH
                     TKKILASYARVAQKIRARADARDTAGLTRAMEDAAKTLF"
     gene            15792..16556
                     /locus_tag="PLA58_02555"
     CDS             15792..16556
                     /locus_tag="PLA58_02555"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOR61706.1"
                     /translation="MDQKIDQVARLAAKAGKIVVFTGAGISTESGIPDFRGPGGLWTK
                     YDPDDFTIDKFLGSHATRKKMWQRLREGGLMEDARPNAAHYAVVELEKMGKLLSLVTQ
                     NVDNLHQKAGSSPALIRELHGNMQRLVCLNCRESYPIAVVKEKYAGSDDAPTCDYCRG
                     ILKPDVIFFGEALPQQALAQAMREAESCDLMIVIGSSLVVYPAAYVPVHAKQSGADLV
                     IINKGETEQDHLADVRIDASAGETMTKILNRLKAED"
     gene            16762..17535
                     /locus_tag="PLA58_02560"
     CDS             16762..17535
                     /locus_tag="PLA58_02560"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HOR61707.1"
                     /translation="MCLILFACNAHPDYRLILAANRDEFYDRPSAPADFWTSHPQVLA
                     GKDLKEKGTWLGVTREGKFAAITNYRDPASWKADAPSRGKLVSRYLTGSAGPEKYLRN
                     VAKKAEAYNGFNLLLGDGADLFVYSNRGDIRKLSSGIYGLSNELLDTPWPKVQRGKKL
                     LKRALAQKGKELEEALFDLLADRRVPPDRNLPDTGIGLEWERLLATMFIKSPIYGTRS
                     STLLLIGKNKRVKLIEKVYNGEEEPWLISRFAFPVEDRT"
     gene            17548..18291
                     /locus_tag="PLA58_02565"
     CDS             17548..18291
                     /locus_tag="PLA58_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOR61708.1"
                     /translation="MKVVYSEEYKQVYSYDPASNPGRIESIEFALAGKVNYVEPLPAT
                     EEEIRAVHTPKHVAEVRREGVYDIAALAAGGAIRAAEIGRFEPCFAVIRPPGHHASAD
                     SAWGFCYFNNMAIALTALKRKNKIQKAFILDFDLHVGDGNINILGKTGYVTILNPAGQ
                     DRNEYLKTVANALDKTDADMIAVSAGFDNHEADWGGLLATEDYTTMGRLVREAARRNG
                     GGCFGILEGGYNHSVLGKNVWAFIEGMSG"
     gene            complement(18288..19820)
                     /locus_tag="PLA58_02570"
     CDS             complement(18288..19820)
                     /locus_tag="PLA58_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR61709.1"
                     /translation="MTLIKTRRILSFAIFILFVLLFAGAEKMSAVLSGILPPLQFVPA
                     LLRTLTRPDVVFLAGLAFILLITMLFGRVYCSFLCPLGTLQDLLMAGSKRLGLRPAPS
                     YAKPLNVLRYGLLALTAATALFGSLSLVILLDPYSLTGRMLTHFLQPLAAHAYNLFIL
                     VLKPFDLYLHPKQTTFIPLAVGATTGGFVLLVSILALRHGRLYCNTVCPVGTLLGLLS
                     RASVFHFALHPKTCLACRRCESACKAACIDPQNAAVDMSRCVGCFNCLDACSSGVIRY
                     QKRQRQPKTSRWSPSRRRLILAGIAAASGFWALNANLRECFSARDAGANPPVTPPGSI
                     SAQRFSQACTACSLCVSACPTRVLTPTLTAFGLSGPLQPKMNYEKSYCDYECDVCGRV
                     CPTGAILPIGLAEKKLTQIGEAELLKDICVVYVDDRNCGACGEVCPTHAIRFTEKETI
                     LYPEIDRQYCIGCGACQLACPTTPRSIVVHARPEHKKAQKYIHPETSGESRTSSDQDF
                     PF"
     gene            complement(19938..20891)
                     /locus_tag="PLA58_02575"
     CDS             complement(19938..20891)
                     /locus_tag="PLA58_02575"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOR61710.1"
                     /translation="MAGEQKFKRREILFRGLSLGLVAGGALIPGKPDFLWAKEKSARI
                     PDLVAVKNGEPDAMFKKAIELMGGMEQFVKKGQTVVVKPNIGWPRVPEVGANTNPLLV
                     KTIVESCWAAGARRVYVFDNVVSPTVSNARNCYQLSGIEEAAKAARAVVVPVDNFSYR
                     EVRIPGGKILKTADVHSLILDCDVFINVPVLKHHSSTHLTIAMKNLMGVVKNRMAYHL
                     TGLDQCIADFCLYRKPDLNVVDAYRVLMSHGPSGPGNPQSADIQMKKTLLISRDIVAI
                     DAAAAKIFGREPESIGYIKIAHAQKIGNMNLKELKIATYAM"
     gene            complement(20923..21327)
                     /locus_tag="PLA58_02580"
     CDS             complement(20923..21327)
                     /locus_tag="PLA58_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOR61711.1"
                     /translation="MKYLSAEEARKFAEQWLPAWSGNHPERLADFYTDDAFYLDPGIP
                     HGVKGKKDLLAYFRKLLAVNPNWVWRQTEGIPLEDGFLNKWLARIPVGSATLEIVGVC
                     FVQLDDAGKIRRNEVYFDRSRLLAEIAGLKNR"
     gene            21558..22706
                     /locus_tag="PLA58_02585"
     CDS             21558..22706
                     /locus_tag="PLA58_02585"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HOR61712.1"
                     /translation="MTVIALILFYLFFPVLAVYLARKNALCNKLGAVVICYICGIVIG
                     NIGVLPANLAPIQNPLMLVAISLALPLMFFSIDVRRWSRLAGKSLLSFGLQTLAILIV
                     AALGFFIFRGRVGEETWKLAGMFVGLYTGGTVNLGAIATALRIDSTLYLAAHTSDVVV
                     SAVYLLFLMTVGRRILLKFLPAFQTAGVVSPADHPDFDSYAGIFEKKTFVPLLGALGL
                     SVLIFITGGLFFLVWHEDIAFVAAILSICTLGIACSFVPRIRNIKMTYQAGNYFILIF
                     CLVVSSMADFNRLVSTAPVMLVYVTFTILICLALHVALARIFRIDADTVIVTSVAGIC
                     SPPLVPMVASALKNKEIVLSGVITGIIGWVIGTYLGISLSYILRGTGA"
     gene            22735..23973
                     /locus_tag="PLA58_02590"
     CDS             22735..23973
                     /locus_tag="PLA58_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR61713.1"
                     /translation="MKLYEFNESYRLFEEAQQYVPNGIYGPRTPAFLTFGSYPAFIRR
                     GEGCRIWDVDGNEYIDYMCSFGTNLLGLKHPKVDAAAKKQWENADCFTLPSDLWLPLA
                     KKIVGTIHGADWCVFGKNGSDVTSYAITLARIFTGKQDILLAHGSYHGSHFWCQPHGQ
                     GVPEEWKTHVHYFEYNSVEDLERVVSEHEGAVAGIMLTPHRHDSMRDQELPAKEFVAA
                     VNRLAQKHRFMIIVDDVRCGFRLELAGSSIHYGFNADIQCFGKAMANGYAISVAAARK
                     EMMDAAKNIFFTGTHYFSGVPFAAALACIDEIQAAGAIEYIRKTGTLLMEGLRQAANA
                     AGLQVNVTGPPAMPYMTFADDPLFEKNRFFCGEAARRGIFFHPHHNWFVCAAMTESDI
                     LRTIDVASECFKLTKEKFGG"
     gene            23975..24346
                     /locus_tag="PLA58_02595"
     CDS             23975..24346
                     /locus_tag="PLA58_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOR61714.1"
                     /translation="MKWYHVGIKAAASEASVQFYRDILGLEVIDRVEIWGKQFVFVGN
                     DAISIEIEQANPGDTQSDPSSMTGLNHFSLIVEDIRELVSQAREKGACVLMDPFHPRP
                     DRLTTFLRDPDGVMIQLIQFV"
     gene            24639..25736
                     /locus_tag="PLA58_02600"
     CDS             24639..25736
                     /locus_tag="PLA58_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR61715.1"
                     /translation="MKKFASLVVVLLVALLVSTGNAQVRAGSFTITPTLGLYTFEGNE
                     DIDQAGPLVGLRLGYNFNKYIGIEGYVHYSQTENNAWSPKKNVDFTGYGGEIIFHLLP
                     NQALVPFLAVGAGGVHYSYGVFEDDSDYGTDNYKDNKFTFDYGYGLKWFLTDWFALRL
                     DIRHVMPIDRFHNNMLASLGLNFAFGGAKKTTATAVSAAEPVVEEAAKPQAAAAPVVE
                     ESRPAVAAAAPVVVEEVVKPQPKTQVREEVDEMGRATLEVLFDFDKATIKKNYTKDID
                     HMVDVMKEHSDLDLTIEGHTDNVGKPAYNKSLSQKRADSVKNYMVEKGGIDAKRLKAV
                     GYGQEKPVASNKTKAGRAKNRRVEAAVDYKK"
     gene            complement(25754..25972)
                     /locus_tag="PLA58_02605"
     CDS             complement(25754..25972)
                     /locus_tag="PLA58_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61716.1"
                     /translation="MSDEKSKDPSLHSGHGFLPSVEMIKMTVIQNSQFVKGLSADFNR
                     QGISPATRATKKTPAEKTAGAPFDLKAF"
     gene            26163..26783
                     /locus_tag="PLA58_02610"
     CDS             26163..26783
                     /locus_tag="PLA58_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61717.1"
                     /translation="MKKIYAVVYIFILALFLLAGCAKAPPVVKKDEPKVKEPPPVPVV
                     VKKPPVAPPPPPPDMEKLPDIVRSVPDSKVWVRYGKTKMGENYYNQTNIARSSNIATV
                     TLYRVVTDLCRKQMIEEVKKYNPKKSVKYHYYEHDVRVDEMDCKNQKWRVKDLIHYDD
                     KGSVLDHHTYENEPWQPIKFVTDHHTLQKSICVAEEKPVVKKKKKK"
     gene            complement(26795..28054)
                     /locus_tag="PLA58_02615"
     CDS             complement(26795..28054)
                     /locus_tag="PLA58_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOR61718.1"
                     /translation="MQQPELLAPAGNLEKLRTALLYGADAVYVGVPGLSLRGTSAEMS
                     LEDLALGVSEAHARSVRVYGAINTFARNADLDQAAYCLPDWAATGVDALIVSDPGLVR
                     LARRRVPQVPLHLSTQSNTTNAEAVRFWQDQGVSRIILARELPLCEVGEIAAAVPGME
                     LEIFIHGAMCMAYSGRCYLSAWRNRKSANSGDCTQPCRWEYRLSESTRPDDPLVLEED
                     DRFSYLLSSKDLCLLEHLPEVLASGVAGVKIEGRMKSSYYVAVVTRTYRQALDALCLH
                     QETYRCDPNWMEELKTVSHRGYTTGFAFAEEKINESSPDIKNIQTHEPAGMVVGYDNV
                     TKELRMEVRNFLQAGEDVELLLTKETLPVDARPMTDDEGNRIDRAHAGNLIRVPLPFP
                     APKGALLRKKTVGAKTQGPARKPSENG"
     gene            complement(28066..28839)
                     /gene="recO"
                     /locus_tag="PLA58_02620"
     CDS             complement(28066..28839)
                     /gene="recO"
                     /locus_tag="PLA58_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOR61719.1"
                     /translation="MSARETHKTAGFVLRSLQYGESDLIVTFYTREFGKLKGIAKGAK
                     RSRRRFANVFEPFSLTQMIFSRRNRDALAFIDSCDILEHFAPIRLDLEKTLTASYFID
                     LTEHFSPEGKRNDKVFVLLADFLSLLTREDVSESMIRFFEMRLLKAVGFEPALTACVR
                     CKTPVTNGAAYIFLPNEGGIFCAACARPERYDQSVSAGAVRTLLLGKDMDLDKMKMVC
                     MTESLASESRSILCGFISHVLGKEIKSLKVLEQVRRYCP"
     gene            complement(28836..>29958)
                     /locus_tag="PLA58_02625"
     CDS             complement(28836..>29958)
                     /locus_tag="PLA58_02625"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOR61720.1"
                     /translation="LIDMHTHLREPGYEYRETVASGAAAAVAGGFTSIACMPNTRPVN
                     DNRSVTEFILRKAAEANLANIYPIGAISMNSDGKQMTEFWDLREAGIVALSDDGNPVM
                     DAALMRRAMEYADSLNLPVIQHCEDKNLSAGGLMNEGYPSTLLGLPGIPAIAEEIMVA
                     RDILIAEYTGTRIHFAHVSTVGSVRLIRDAKKRGLQVTAETAPHYFTLTDESLRDYDT
                     NYKVSPPLRGKTDVSAVKEGLADGTLDAIACDHAPHGRTDKEVEFEYAANGISGLETS
                     LGLSLSLVHEGVLSWPELIAKMSCNPARILNLPKGTLAKGADADITVIHPELAWSVDA
                     AAFHSLGKNSPFSGRKLKGRAVLTIVGGDIKYDGLASKP"
BASE COUNT         6612 a         7847 c         8892 g         6407 t
ORIGIN      
        1 ttccctttga cggcaacctg gaaaacgtcg ccggctttga cgttttccgg ccagccgggg
       61 tacgaaacaa tatttttcgg agcgcccatg acttcaacct ttgtttttct cgcgggaatc
      121 gacagctttt ccaactgcgg caggcagaat tgatacatgg cttcgggccc gcagacaaag
      181 aatgtccagt ccgttccttc aagctttttc ccgatcaggt tggcgtcgat gaatccccgg
      241 catccgctga aggaaggatc cggttcggac acgaccaggt cccacgtgaa gttcttgtga
      301 gcttcggcaa tccgttcgat ttcctgccgg taaatcacat cctcctctac gcggctgccg
      361 taaaacagat gcagccggcg gtccaggttt cggtcggcca tctcacggat catgctcatg
      421 aaaggcgtga tgccgctgcc gcccgcgatg aacgccagcc ggtttccgtg gaaaagcgga
      481 ttgtagtgga agttccccgc gggcgatgag gcttgcagcc ggtcgccggg cttcagttca
      541 tccagcagaa cattggaaac aaatccgtct tccacgcgcc ggaccgtgat ttcgtaatag
      601 ccggtctgcg tgggggagga cgcgatgctg tagggcctgc tggtcctgat gccgcctgcc
      661 tccaggaaaa cattgatgta ctggccggac tgaaacggcg gcagagcgcc gttgccgctc
      721 accagtctga aggatttggc gctcgcggtt tgctccctga tttccgacac gatcagatcg
      781 agcctttgcg ggtgcagcga atcgatgatc ttttgaactt ccccctttct ggcgggatag
      841 tcgaatccgt acttctcgag aatcgtattg tcccggatga tttcatcgta tccggctact
      901 ttttgcatga tttcttttct ggccatattt tcccccttac tgccttttca gaagtcgtga
      961 aacctgccgc gccgcggaag cgcccgattg aagcgtcggc tgaaagccgc cggagccgtt
     1021 ccatgcgccc gtgaaataca gcccctcgat cgccgatttg gaatcgacnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aacctggcag gcgccgcggg gtgaaaaatc
     1201 cggatcgtca acatcatagc acgtcagcag ggagtactcc gccccgtcca gcgttttcat
     1261 tttgctgtgg gcgtaatccg cgtccgctgt gccgcagatg aagttggtgg ccttgtcgat
     1321 tcccatctcc gaaggatcgc agtcgaagcc catatacagc gtgaaggccg acagtccaac
     1381 cgtgcgggcg ttgagctttt tcagctcgtc ttccggacag ttttccgccc cgaccatgtc
     1441 gatgagcgtg cgaatcggac cggcgttgga caccacgaat tttgtcgaaa tttcatcccc
     1501 atcctccgtg acgacggaga cgacttttcc gttctgaacc cggatctttt tcgccgcgca
     1561 gttgaatcga gtatccccgc cgaagcggag aaaggcgtcg cagatcgcat tggacatggc
     1621 ctgcgatccg cccttcatgt gccagggttt gaactcgatg tacgcccaga tcaccatggc
     1681 cagctccatg aaactcagcc ggctcggcgg cagccccgca tagctccagt aaatggttac
     1741 gacggtctgc agcagggggt tcttgaggta ttggtccaga atgccctggg agttttgcag
     1801 cgccagtttg aaaaagagcg gatatttttc gggactggcc tccggatcat gaatgaacag
     1861 cacgttcacc agttcgttgc agaacgcata gagaaggtca aaaaactttt gaatcccttc
     1921 cttttccgcc ggaaattttt ctatcagcgt ctcgatgacc gcctgtctgt cggctttcag
     1981 attgatgtcc attcggccgg ggatggcaac ccggtagagg ttttgttgct ggataaaatc
     2041 gattttatcc agcacgccca gttcgccaag cagccccctc agggggccgg gaaattcggg
     2101 aagtcccatg ccgctcaact ggtgaagcgc aatttcgaac tcgaatctgc ctcttacgaa
     2161 gcttgtcgcg catccacccg gaacattgtg tctttcgagc agcagggtct tgtgcccttt
     2221 ctgggcaagt cccgccgcag ccgtcagccc gccgttgccg gcgcctatca ccacgacatc
     2281 ataatccgac ataagccttc tcctctccga aaataaaaat attcaggccg aaatcccggc
     2341 cggtcttcag actaaacgat ctcttgcgca ttcaggaaac ggatttcttt cgctggcgag
     2401 tatagtgaaa cccttcgtct ctgtcaaagg aaaaccgccg ctcatgatct ttccatttgc
     2461 gtcggtcatt ccttgtttct cctgttttat cgattcattt catcggaata ttttaattgc
     2521 agagtgtgaa tgtccggaat gccgctgcgg ccgcccattt gttcgctcac gcgcgcggct
     2581 gcccaggcgc ccaggtccag gcatttttcc ggactccacc cgtgaaggat tccgtgaatc
     2641 aatcccgcgt gaaaaacgtc gccgcaaccc gtcgtgtcga ttgctttggc cggataagct
     2701 tctttttcaa tgaagcaccc gtcaaccagg gccacatacc ctctctttcc cagcgtgacg
     2761 ccggccagcc gcacgcccat gccggcgagt ttgcggcagg tttcggcggg cgtttcggca
     2821 aaagcgttgg agaatacttc cgatgtgaca aagcagtcgc tcattttcgc catctccagc
     2881 atgccgtcgc gcagggttcc cgcgtccaca atgacgggaa tccccgcttc ctttgccttg
     2941 cggcaggcga aaagcgaggc ttcggtaaac agcccgtcgg tatgcagcgc ctggctcgaa
     3001 agcaggatgc cggtgttgat ttcctccggc cggagcggtt ctcccgtggg catctggtaa
     3061 aagatcgtcc gacgggccag cgccggttcg ctggcgataa acgcgaactg ggactgatgc
     3121 tgtttgcgga tttgcaggcc ggacgtgtcg aggccttcgg cgtccagcag cgccttgatt
     3181 tgtgttccaa agccgtcgtc gcccaggacg ccggcgatgt ggcagcgggc gccccagcgt
     3241 ttgagcgcga ccagcgccgt ggcggcgggt ccgccgccct gcaagagcag gttggtgaat
     3301 tcacatttta catccggagg gggataagcc gcgaccagac caagatgatc cagagagcac
     3361 tggccgatcc cgaaaacaag gggggctttc atcatggcaa tgtccttaat tttttgcgtt
     3421 caataccatg caacactgga atgtgaaaat aaaaaagcgt acccgcaaga taattattga
     3481 taagaatttc aagatgaaat aaaaataatc gaagggcgat aatatatacg gcgtgttcgg
     3541 ggaacccgcc ctacggggaa tttgttgtag ggattgatcc ccgatcaatc cgcgggctaa
     3601 cggcgctcgg agatcgcgtc ctgcaactaa cgcggtaaac tgtgatatgt agggactgtt
     3661 ctccgagcag gccgcaaaac agctgtatgt tcagggttca cgctcgcagg acaacatcaa
     3721 aagaaatcaa aactattgag aggcataaaa tgaaagtgct gttaatcaat ggaagtccga
     3781 acaaaaaagg atgcacctat accgcccttt gtgaagcgga aaaggagttg aacaagcaga
     3841 agattgaaac ggagatcctc tacctcggac agaagcccat cagaggatgt acggcatgcg
     3901 ggaaatgtct ggagacggga aaatgcgtct ttgacgacac ggtaaacgtc gcccttgaaa
     3961 aggccgctca atccgacggt tttgtttttg gttctccggt ccattacgcg ggagcgtccg
     4021 gtttcatcac atcctttctg gaccgctgct tttttgccgg cagtgatgtc tttgcctaca
     4081 aacccggcgc ggccatcgtg agctgccgca ggggcggcgc caccgcggcg ctggaccagc
     4141 tcaataaata ttttaccatc aacaacatgc cggtggtgtc atcccaatac tggaatatgg
     4201 tgcacggcaa cacaccgcag gaagtgcttc aggaccttga aggaatgcag accatgcggg
     4261 ggctgggccg caatatggca tggcttttaa aatccattca ggcgggtaaa aaatcgggag
     4321 ttgcgctgcc ggcgccggaa gaacggatat ggaccaattt catcagatca tgaatgcagt
     4381 tgccatagtg gaaggggcag catcgttggg aatatttatg aagtccggtc aaagcgccca
     4441 ttttttaatg gcaataaaaa ggcaattaca ttagaatcac accatgagtg acgaacgaga
     4501 aatcataaaa tactggtcga ccacctcaaa gcaagatttt gaaacagcgg aaattctctt
     4561 tgagaaccaa aagtacagcc acgctttatt tttctgtcat ttgagcatag agaaaattct
     4621 taaagcgctc attgtcagga aaacacatgc agctcctccg cttgtccatg atctggtgcg
     4681 cttagcggaa aaaacgggca tcagcttatc ggataagttg aaaggtgagt tggcggagat
     4741 cagttcattt aatattcagg cgaggtacga cgattataaa ctaagctttt acaagaaagc
     4801 gaagaagcga tttgcggcaa aatacattgc tgcaacaaga gagattttaa tatggctaag
     4861 agcgcaactt taaaaaagaa agaaacggat cttatcaacc gttatgtcct gttgctcagg
     4921 caaaaaggaa tcgacgtatc taaagtagtg ctgttcggtt catacgcaaa aggatcggca
     4981 aagccggaga gcgacatcga tctggcaatc gtttcttctc aattcggcaa ggatcactgg
     5041 aaagaaatgg tccttttacg aaaattggct ctggaaattg attcgcacat cgaaccactg
     5101 ccctttgcgc cggaagatgt ggaagatcgt tacagcgcgc tttcgcaaga aataaaaaaa
     5161 accggaatat tgcttaagat ttagcgcaaa agcgtgaatc gttgacaatg ccgttttgta
     5221 ttgctacata aaaatgcaaa ttcggggtgg cagaaaacaa aatctgtctg agatgatacc
     5281 cgttgaacct gatccggata atgccggcgg agggaggata gagaaaaaaa tgaacccttt
     5341 cttccggaca cggcgaaagg gtttttttat ttaacaaagg ggagtgcaag ccatggcaat
     5401 aacggcggcg gagattttta aatcggtgga ggatatccgg gccgaaaggc cgctgattca
     5461 caacatcacc aattatgtgg tcatgaacaa cacggccaac gctcttctgg caatcggcgc
     5521 atcgccggtc atgatccatg ccgaggaaga agtggaagac atggcggcgg ttgccggcgc
     5581 gctggtcatc aatatcggaa cgatcagcgc cccgtgggtt cggggcatgt tcaaggcgtt
     5641 tgccaaagcc gcatccagag gggttccggt ggtgatcgat ccagtcggcg caggggccac
     5701 accttacaga accgagaccg ttcgggatct gatccgatcc ggccggccga cgattatccg
     5761 gggcaacgcc tccgaaattc tggcgctgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnc gatccggccg gccgacgatt atccggggca acgcctccga aattctggcg
     5941 ctgagggacg accgcctgaa aacgaagggg gtggacagca ccgctgcctc cagtgaggcc
     6001 ctttacgccg cgcaaagcat cagcaaaacc tatcggtgcg cggtgtgcgt cagcggcgcc
     6061 gttgactata tcgtagaagg aaaacgtctg gtaaaagtag ccaacggcca tccgatgatg
     6121 ccgaaagtga cgggcctggg ttgtacggcc agcgcgcttt gcggagcttt tgcggcggtg
     6181 gatcgggatc cgctgacggc ggcggccaaa gccatggcgg tcatgggcat tgccggagag
     6241 atcgccgccg aacaatcgcc cggtcccggc tcgctccaga tgcatttgat cgatgcgctg
     6301 taccgtttga cgaaagagga catggagcgc cgtctgaaag tggagggata gcggtgcgcg
     6361 gcatctatct ggtgacggac cgcgggttgt gccgcggcag actgcttctg gacgtggtgg
     6421 cgcaggcggt caaaggcggc gcagcctgtg tgcaactgcg tgaaaaggag atgcccacgc
     6481 gcgcattcgt ggaggaagcc cttgccgtga aaaacatcct tgcaccccac cggattcccc
     6541 tgatgatcaa tgaccggatc gatgtggcgc tggcctgcgg cgcggacggc gttcatatcg
     6601 gtcaggagga tatgccgtat gaaacggccc gccgcctgct ggggccgaaa gcgatcatcg
     6661 gtctgtccgt ggaaacctgg gcggatgtcg aggcgagtcg tgatctggat gtcgattacc
     6721 tcggtgtcag ccccgttttt gccacgccga ccaagaccga tacgaaagag ccgtggggac
     6781 tcgaagggct cagaaaaatc aaggcattca gccgccatcc cctggtggcc atcggaggaa
     6841 tcagcgaggc aaacgcctgc gaagtgactg aagcaggcgc caattgcctg gccgttgttt
     6901 cggccatttg cagcgccgat gatccggccg gagcgaccgc gagactcaaa catgtctttg
     6961 atgaggcgct gcgccgtcgg ggaaatcggg attgaaaaat tttcttgaca agcccggagc
     7021 gctcgtttat agggcaggcc aagaaagagg acggtttttt tcaatgaaca gacagatcaa
     7081 tgcaaacagc aatgcttttg gctattggtt ttattacgat gcgtcggtct gcctgtcgct
     7141 ggggaggact tggtaaggta acaaagccaa aacagccatg gggagatcga gcgaaagctg
     7201 cccatggctt ttctgtttta aaaggccatg gcggcgaaaa gccatggcct tttttttcaa
     7261 gggggatgta tgattattgt catgaagagc cgcgcaacgg aaaagcagat cgagaatgtg
     7321 attgcctgga ttgaatcggt cggctacaag gcgcatccgt ccagcggcgt ggaacgaacc
     7381 attatcgggg cggtcgggga ccaacgcgac aaagccgcgc tgaaatatgc ggaaaacctt
     7441 ccgggcgtcg aaaagaccat gccgattctg aagccttaca aactggccag ccgggaattc
     7501 catgaagaag acagtgtggt ccgtgtgggc gccgccgaga tcggcggacc ggaattcgtg
     7561 gtcatggcgg ggccctgcgc catcgaaagt gaagaacagc ttctggaaag cgcctatgtc
     7621 gtcaaaaaag gcggcggcca gattctgagg ggcggcgcct tcaagccccg cacgtctcct
     7681 tacagcttcc agggcatgga ggaggaagga ttgaaagtcc tggagtccgt gagcgggaaa
     7741 accggcctgc cgacggtgac ggaagtggtc aatcccgcgg atgtggatct ggtggaatcc
     7801 tacgcggata tgctgcaaat cggcgcgcgc aatgttcaga atttcgcgct gctcaagaaa
     7861 gtcggccacg cgaggaaacc tgtgctgctg aagaggggaa tgatgacgac catcgaggag
     7921 ctgctcatgt cggcggaata tatcctttct tccggcaatc ccaacgtggt cctttgcgag
     7981 cgcggcatcc gcacgttcga aacggccacg cgcaacacgc tggacatcag cgccgtgccg
     8041 gtgctcaaaa gtctgacgca tctgccggtg gtgatcgatc ccagccacgc ggccggcaac
     8101 tggaaatatg tgattccgct ttcccgcgcg gcgctggccg tgggcgcgga cggcatcatt
     8161 gtggaggtgc acccggagcc ggaaaaagcg gtttccgacg gggcgcaatc gctcaaaccg
     8221 gaaaaatttt atatgttaat ggacgacgtg cgcgcgcttg ccaaagccat gcgcggctga
     8281 tccagggggg aaccgatatg aaaatgttga aagtccatct ggataaaaaa gctctttctt
     8341 cccatgagat ccgcatcgga cggaatattc tggaccgggt ggttctgctg ctcgcgaaaa
     8401 accataaggc ttcgcgctac ggaatcgtga cggatacgca tgtgaatcgt ctctttggaa
     8461 aaacgctgct ggaatccatg tccggcgctg gtttgaacac atttcttctg gaaattccgg
     8521 cgggagagtc ttccaagacg attgcgaccg cgatggatct cgccggacgt ctgctggaag
     8581 ccggcgccga tcgcggaacg ctgctgatcg cgctgggcgg cggcgtggtg ggcgatctga
     8641 cgggctttgt cgcctcggtc tatatgcggt cggtcccgta tgttcagata ccgaccaccc
     8701 tggttggaca ggtggacagc gccatcggcg ggaaaaccgc cgtggatctg ccgcagggaa
     8761 agaatttgct ggggactttt tatcagccgc gggcggtgtt tgcggattta aggtttctcg
     8821 acacgctgcc ggagcgggaa ttcgtgggcg gtctggccga aatcatcaag tacggcatca
     8881 ttgatgatga aaagttgttt cagcggctgg aagaggatat ggaagcggtc aaacgcaggg
     8941 atcccgaacg gctgctgacg ctggtcgaaa cctgctgccg gatcaagaaa tcgatcgtcg
     9001 agatggatga gcgggagcag gggctgagaa gaatcctgaa tttcggccac acgctggggc
     9061 atgccctgga gaccgcgtcc ggctacgcgc tgtcgcacgg tgagggcgtg gcgctgggca
     9121 tgatcgcggc tgcccggctg tcggtgcaaa tgaaatatct gccccagagc gaggcaaaaa
     9181 gggtggaaaa ggttattgcc ggggcggggc ttcctgtgcg aatcgccgct acccttgata
     9241 cgaaagctgt tatggacagc ctgaagatgg acaaaaagaa aagaaataat gtggtccatt
     9301 ttgtcctgat ccggaaaatc ggcatgccgt ttgtcaacgg cagcatccag gatgagacga
     9361 tcctcagtgt tctggagggc atgaaggcat gaaacaaccg ttggaaacgt tgcgggaaga
     9421 gatgagaaag aaggaccggg acattgtccg gctgctcaac gaacgctcgg aaatatccgt
     9481 gaaaatcggc cgggtgaaag ggcaggccgg tttcccggtg tacgatccgg cgcaggaagc
     9541 cagggtgctg cggtatctga gcgaactcaa cggcggcccc ctgtcggaca gtcaggtcac
     9601 gtccattttc cgggagatcc tctcggcatc ccgcgatttg cagaagccca cgcgcatcgc
     9661 ctaccttggg cccgaggcgt ccttcagtca tcaggcctcc cttctgcatt tcggagcctc
     9721 ctgtcaattc ttcccgcagc agggcatcgc gcgggttttc gatgaagtag agaagggcga
     9781 agtggaatgg ggcgtcgttc ccgtggaaaa ttcgctggag ggttcggtca atgtcacgct
     9841 ggaccggctg gcgctgacgc cgctgacggt tcaggcggaa attgatcttc gcatcagcca
     9901 gtgtctgatt tcgcaggcga aaagcctgaa aggcatcaaa agggtctatt cgcatccgca
     9961 ggggctggcg cagtgtcagt cgtggcttcg cgcgcatctg cccaacgcgg cgacgggtga
    10021 gatggaaagc acggcggcgg ccgtcgcgat ggtgcggggg aaaaaaacgg cggcggctat
    10081 cggaagcgcg ctggcggcct ccacctacgg attgaacctg ctggcccggg gcattgaaga
    10141 caattcctcc aacacgacgc gttttctggt gatcggccgg ggcgacacgt tccccaccgg
    10201 cgatgataag acgtcgatca tattcgccac accggacgtt cccgggtcgc tgcaccgggc
    10261 gctttcgtgt tttgcgaagc gcaggatcaa tctgacgaaa atcgagtcgc atcccgtgaa
    10321 agagaggctg tgggagtatt tcttttttgt ggacatgatc ggtcatctgc gcgaccggtc
    10381 agcgcagaac tgccttgcgg aattaaaaga caaaaccatt tatctgaaaa cactgggggc
    10441 ctatccgcgg gccaaggacg gaagatgatc tgcataccgg tcatggaccc gacgcatcgg
    10501 gaggctctcc ggtcggcgca aaaaagcgca agtctcgccg atctgatcga attgcgcatg
    10561 gattggatcg cggatggaga cgtgtcggag ttgatccgcg cggcaaggcg cgcctcggcg
    10621 agcgtcaaaa ttattgtgac gtgccgcagg agggaagaat ctgaacttgc gcgggaaggc
    10681 ctcgaagccc tttccgtgaa agaaagaacc aacgcggcca aaatgaagct gctgcgggag
    10741 gctgttgtat cgggagcgga ttttattgat atcgagcttg cggaagggac cagagccgtt
    10801 tcgcggatgc gatcctggtg ccacagccgc ccaaatcctg cgcggctgat cgtttcctgg
    10861 cacgaccctt acaaaacccc gtcgctggcc tctctgaaga aaatctttga ggcctgtgcg
    10921 cgggccggcg ccgacatcgt gaaaattgtt tcgtacgccc gaagtctgtc cgataacgcc
    10981 gccgtgctga atctgattga ctatgcgaaa caccaggggc gggaaatcat cgccatgtgc
    11041 atgggggaaa aggggcagat cagccgggtg ctggcgcctt cgcggggaag ttatctgggg
    11101 tttgccgttt taccgggcgg cagggcttcc gcacccggcc agttgacgct cagggagatg
    11161 aggacttttc agaaacttct gcgcccgcat cgtccatcga ttgagccgcg gcagaaccaa
    11221 cttcatttcg tccttctggg aaacccggtt cgccagagcc tgtcgccgct gatgcatcag
    11281 gcggctttcc ggacaatggg catcgacgct tcttacagcg ccttttgcat cgccgatccg
    11341 gcgggcgccg tatccggcat tcgggcgatg aacatccggg gtgcatcggt cacgattcct
    11401 ttcaagacgg cggtcatgga gtatcttgac gacatcgacg cggacgcagc cgctgtgggg
    11461 gcggtcaaca cgatcgtgaa cgattgcggc cgcctgatcg gccataacac ggactggctg
    11521 ggactgatgg cggcgctgaa ggaaaaaacg cagatcaggg gcaggagatt tgtgattctg
    11581 ggcgcgggcg gcacggcgcg ggcggccgca tacggaatcc ggaaagaagg cgggcggccg
    11641 gtcattgtca accggacgcc ggagaagggc atggcgctgg ccatgcagtt tggctgtccg
    11701 ttttatccgt tggcacagat cagtaaaatc aaggccttcg gccttattaa cacgacgccg
    11761 gtcggcatgt ttccggatat acaaaaatcg cccgtccctc cgtccgttct tgcgaattac
    11821 cgggtcgtgg cggatgtcgt ctataatccc ctgaagacga aattgcttcg ggatgcggaa
    11881 gcgaagggct gcaaggtcat cccgggactg gaaatgtttg tcaggcaggg agcggagcag
    11941 ttgatgttat ggacgggcag agcagcgccg ctggcggtga tgagaaatgc cgtccgggaa
    12001 aggttaatgc agcatgaatc ctgagaaaga cctgccggga aaagtttcca gcgtggaagt
    12061 gccaggttcg aagagcctga cgcagcgggc tctgattgcc gcggcgctgg cccgggggga
    12121 atcgttgatc cggggcgctt taatcgcgga ggacacccgt catttgatgg aaggactgaa
    12181 tctgctgggc gcccgtctgg agggggttga cggcggaatc cgggttgtcg gaaccggcgg
    12241 ggcgattgcc aatccggacc gtgcaatctt tctgggaaac aacggcacgg cgctgcggtt
    12301 tttaacggcg ctggtttgcc tgggacgcgg gacgtatgtt ctgaccgggg agccgcgttt
    12361 gctggaacgg ccggtcggcc ctctggttga ggccctgaag ggcatggggg taaacatcgc
    12421 gtgcaaaaac cagtgcccgc ctgtcaccgt atcggccaac ggactgaggg gggggcaaat
    12481 tacactgacc gatatcgaaa gctcgcagta tgtttcggcg ttgctcctgt gcgctcctta
    12541 cacagccaag gggatcgagc tttccctcac gggaaatatt gtctccgcgc cttacattga
    12601 tttgacggtc ggcgtcatga aggatttcgg cgcaaaaatt taccgcacgg gacgcgattt
    12661 gttcagcgtt ggaacccagt cgatttacga gggtcgggcg tatgatgtgg aaggagacgt
    12721 ctccagcgcc tcgtattttt ttctggcggc ggcccttttg aaaaggccgg tccgcgttta
    12781 cggcattcgc cgggagagcg cccaggggga catcggcctg ctggatgttc tggagaaact
    12841 gggctgccgg atcgaaagcg gcgaggcgtg ggtggaagtt tccgcgccga acagccttgc
    12901 cgcaggcgat gtgacgctgg acatgggaaa tatgccggac atggtgccga cggtcggggt
    12961 tctggcggcg tatcgcaggg gaaagacggt gatgaccaat gccgcccatt tgaaaatcaa
    13021 ggaaagcaac cgcctggcgg cgatggcaag cgaactgagc cgtgtgggga tcgccgcgtc
    13081 cgaacggccg gacggtttga cgatcgaggg aggcattccc catgccgcgg acattgaaac
    13141 gtacaacgat caccgcattg caatgagttt tgccgttgcg gggctggcca cgccggggat
    13201 tgaaatcgcc gacaaaaaat gtgtggacaa atcttttccg ggcttctggc gggagctgga
    13261 caaattatga aaatcatcct gatcggctgc cgcgcgtctg gaaagtccac cgtcggcagg
    13321 cttttgtcgc gaaaactcgg cgtgcctttc gtagatgcgg atcaactcgt cgaaaaaacg
    13381 gcgggcatgt ccatcgcgga aatggtcgcg ttgaaaggct ggcaggaatt ccgcaggaga
    13441 gaaacgcaga cgctggcttc tcttcaggat ccgtcggttt gcgtcctggc caccggcggc
    13501 ggagtggttt tggcggatga aaaccggagg ctgctgaaaa aaacgggtgt cgtgatttac
    13561 ctgaaagcag acctgccgga tgtcgtggag cggctgcgcc gcgacgcgaa aaatacacag
    13621 agccgcccgc cgctgacaga cggagatctg atcgaagaaa cccgctctgt gttattgcag
    13681 cgagccccgc tgtatcggtc ggttgccgat tacacggtgg aaacgcgaaa caaaaatgcc
    13741 gaacaggcgg cggaagagat ttatcggcag cttctggagg cgggcatcat gtcaaaaatg
    13801 cagaccttga aacagaagac gatcgaataa cgattattgg gggacgtatg gcaggcaata
    13861 ggtggggaac cttgttttgc gttacgacgt ggggggagtc gcacggcccc gcgctgggcg
    13921 cggtgattga cggctgtccg gccgggatcc ggctgactcc cgaagagata cagcgggagc
    13981 tggaccgacg caggccttcc acatcagcgg cttcgacgtc ccgcgccgag aaagaccggg
    14041 tggaaatcct ttccggcgtt tttgagggtc aaaccaccgg cacgccgata gcgctgctgg
    14101 tccggaatac cgacgcgcga tcggagcatt acgacgagtt gaggaatgtt tttcgccccg
    14161 gccacgggga ttacacgtac tggaaaaaat acggcctccg ggactggcgc gggggtggac
    14221 gcgcttcggg acgggaaacc gtcgggcgcg tcgcggcagg cgcggttgca cgcaaagtgg
    14281 tccgggccgc cggcatggag gtgattgcct atacccggag catcggcggc atcgggattt
    14341 cgccggatgc tctgacgcgg gatcaacact gggaaaaaag agtccgcgaa agccccctgt
    14401 attgtcccga tccgcaggcg gcgtcggcca tggaggagcg cctggctgcc gtccggaagg
    14461 aaggggacac gctgggcggc gtggtggaaa tccttgtccg gggatgcccg gccggtttgg
    14521 gggagccggt cttcgacaag atggatgcgg acctggccaa agccctgatg agcatcggaa
    14581 cggtgaaggc cgtggaagtg ggcgagggct gtgcggtcgc gtcaaaaaat gggtccgaga
    14641 tcaatgatcc gatggatgcc gcaggctttg tcaaaaattc cgccggaggc atcctcgcag
    14701 gcatcaccac cggtcaggac cttgtttgcc gggtgtactg caaacccatc ccctccatcg
    14761 ccctgcccca gcagacggtg gacgaaagcg gccgggagcg ttcgatccgg gtcggcggca
    14821 gacacgatat ttgcgtgatt ccgaggatcg ttccggtttg cgaggcgatg gtcctgctgg
    14881 tgctggcgga tcactggctt cgacagaaag cgggtggccg atgaaaaaaa tgacggcggg
    14941 cattatcggc ggaacgcacg gcatgggccg ctggatggcc ggactgctgc gcgcgcaggg
    15001 tttggccgtg agcgtggcgg gccgggcaac aaaaaccacg gcggtggatc tggccggccg
    15061 atgcgatgtg gtggtggttg ccgtgccgat tgcggcgacg gcaggtgtca ttcgggaagt
    15121 cgggccgagg gtgaaacccg gccggctgct gatggattta acgtcgctga aaacagagcc
    15181 ggtggcgttg atgctttcgc attccgaagc ggacgtggtc ggctgccacc cgctgttcgg
    15241 tccgtcggtc agcgatccag ccggacattc cgtggttctt tgccgggggc ggggcgagaa
    15301 cggctatgct ttgatgaagg ccgtttttga aaaggccggt tatcgggttt tggagacaac
    15361 gccccggagg catgacaaga tgatgtcggt cgttcaggcc ctcaaccatt tcaacaccat
    15421 cgtcctgggt atggcggttg ccgaagcggg gattccgttt ccggagctgc agccgttttc
    15481 cacgccgatt tttacaacca aaatggagat cgtccgaaag gtatttacgg acacacccgg
    15541 actttatgcg gatattcttg ccgggaatcc ccacacgaag aagatattgg cgtcttacgc
    15601 gcgcgtcgcg caaaagattc gcgcccgagc ggatgcccgc gacaccgcag gcctgacgcg
    15661 ggccatggag gatgccgcaa agacgttgtt ctgaggcggg ccggacggaa gatttccttt
    15721 ggatttttga ggcggatggt gtatttttgt cacgctgcat aacgtttcga tcaaaggagc
    15781 ggccttcttt tatggatcaa aaaatcgatc aagtcgcgag actggcggcg aaagccggaa
    15841 aaattgtcgt cttcaccggc gcaggcatca gcacggaatc cggcatccct gattttcgcg
    15901 gtcccggagg actctggacc aaatacgatc cggacgattt caccatcgat aaatttctgg
    15961 gatctcatgc aacgcgcaaa aaaatgtggc agcgcctgcg ggaaggcggc ctgatggaag
    16021 acgcacggcc caacgcggcg cattatgccg tcgtggagct ggagaagatg ggcaaactgc
    16081 tgtctctggt tacgcagaac gtggacaatc tgcaccagaa ggcggggagc tcgcccgcac
    16141 tcatccggga attgcacggc aatatgcagc ggctggtttg cctgaactgc cgggagagtt
    16201 acccgattgc cgtggtcaag gaaaagtatg ccggttccga cgatgcgccg acctgcgact
    16261 actgccgggg catcctcaag ccggatgtga ttttcttcgg agaggccctg ccgcaacagg
    16321 ccctggcaca ggcgatgcgg gaagcggaaa gctgtgattt gatgatcgtg atcggttcgt
    16381 cgctcgttgt gtatcccgcg gcctatgttc cggttcatgc caagcagtcg ggggccgatc
    16441 tggtgatcat caacaaaggc gaaaccgaac aggatcacct ggccgacgtc cgcattgacg
    16501 cctcggcagg ggagaccatg acgaaaatac taaacaggct gaaggctgaa gactgaaggt
    16561 aaaggaacgg gcgcgaaacg cgccgtcatg ccggcgaata ccctggaggg cacgagtgcc
    16621 ggcatccaga aaaaattaac cacgtcgcag caaactgcca agaagtaaca ccccatcccg
    16681 caacaggggg atttaaaaag ccgaaggctt acaaaacaga tcttcagcct tcagtcttca
    16741 gccttcggtc taaggatcct tatgtgcctg atcctttttg cctgcaatgc gcatccggac
    16801 taccgcctga ttctggcggc caaccgcgat gaattctacg accggccttc cgcgccggcg
    16861 gatttctgga ccagccatcc gcaggtgctg gcgggaaaag atctgaaaga aaaagggacg
    16921 tggctgggcg tcaccaggga aggcaaattt gccgccatta ccaattatcg tgaccccgct
    16981 tcctggaaag ccgacgcgcc gtcccgcgga aagctggtca gccggtatct gaccggatcg
    17041 gccggtcctg aaaaatatct ccggaatgtt gccaaaaaag ccgaggctta caacgggttt
    17101 aaccttcttt tgggcgacgg tgcggacctc tttgtgtatt ccaaccgggg agatatccgg
    17161 aaactctcat ccgggattta cggcctgagc aatgagctcc tcgatacgcc ctggcccaag
    17221 gttcaacggg ggaaaaaatt actgaagaga gccctggccc aaaaaggaaa agaactggaa
    17281 gaggcgctgt ttgatcttct tgcggaccga cgcgtccccc ccgaccggaa tctgccggac
    17341 acgggcatcg gcctcgaatg ggaaaggctg ctggcgacga tgtttatcaa aagccccatt
    17401 tacggaacgc gctcttcaac actgctgctc atcgggaaaa acaagcgagt caaactgata
    17461 gaaaaagttt acaacggcga ggaggagccc tggctgatca gtcggtttgc gtttcctgta
    17521 gaggatcgga catgaaagga ggatgccatg aaggtggttt attccgaaga gtataaacag
    17581 gtttactcat acgaccccgc ctccaacccc ggtaggatcg aatccatcga attcgctctg
    17641 gcggggaaag tgaactatgt ggagccgctg cccgccacgg aagaagaaat ccgcgccgtg
    17701 cacacgccga aacacgttgc cgaagtccgg cgcgaaggcg tctatgacat cgccgcgctt
    17761 gccgcgggcg gagcgatcag ggccgcggaa atcggacggt tcgagccttg tttcgccgtg
    17821 atccgccctc cggggcatca tgcctctgcc gacagcgcct gggggttctg ctatttcaac
    17881 aacatggcca ttgccctgac cgcgctcaag cggaaaaata aaatccaaaa agcctttatt
    17941 ctggattttg acctccacgt cggcgacggc aatatcaaca ttctcggcaa gacggggtat
    18001 gtgacaattc tcaatcctgc gggtcaggac cgcaacgaat acctgaaaac cgttgcgaac
    18061 gctctggaca aaaccgacgc ggacatgatc gccgtttcgg ccggattcga caatcatgaa
    18121 gccgactggg gcgggctgtt ggcgacggaa gactacacga ccatggggag gctggtccgg
    18181 gaagcggcgc ggcgcaatgg gggcggctgc ttcggcattc tcgaaggcgg ctacaaccat
    18241 tctgttttgg ggaaaaacgt ttgggcgttc attgaaggta tgagcggcta aaaggggaaa
    18301 tcctgatcgg atgacgtccg ggattcgccg gaggtttccg gatggatgta tttttgagcc
    18361 tttttgtgct ccggcctggc gtggaccacg atggagcggg gcgtcgtcgg acaggccagc
    18421 tggcaggcgc cgcagccgat gcagtactgc cggtcgattt ccggatacag gatggtttcc
    18481 ttttcggtaa agcggatggc gtgcgtcggg cagacttcgc cgcaagcgcc gcaattccgg
    18541 tcatcgacgt atacgacgca gatatccttc agcagttcgg cctcgccgat ctgcgtgagt
    18601 ttcttttcgg caagcccgat cggcaaaatc gcgccggtgg ggcagacccg gccgcagaca
    18661 tcgcattcat aatcgcagta actcttctcg tagttcattt tcggctgcag cggaccggat
    18721 agtccaaacg ccgtgagcgt cggcgtcaac acgcgggtcg ggcaggcgct gacgcacagg
    18781 gagcaggcgg tgcaggcctg tgaaaaacgc tgcgcgctga tcgatccggg gggagtgacc
    18841 ggcgggtttg ccccggcgtc gcgggcgctg aagcattcgc gaagattcgc gttcagcgcc
    18901 caaaagccgg atgcagcggc aatgcctgcg aggatgagcc tgcggcgcga aggagaccag
    18961 cggcttgttt tgggctgccg ttgtcttttt tggtagcgaa tgacgcctga tgagcaggcg
    19021 tcgaggcagt tgaaacagcc gacacagcgg ctcatgtcaa cagcggcgtt ttgcggatcg
    19081 atgcaggctg ccttgcaggc cgattcacac cgacggcagg ccaggcaggt ttttggatgt
    19141 aaggcaaagt gaaaaacaga cgcacgcgac agcaggccca gcagcgttcc caccgggcaa
    19201 accgtgttgc agtaaaggcg tccgtgccgg agggcgagga tggaaaccag gagaacaaac
    19261 ccgcctgtcg tggccccaac ggcaagaggg ataaaagtcg tctgtttcgg atgcaggtaa
    19321 agatcaaagg gcttcaggac cagaataaac aggttgtaag catgggctgc gagcggttga
    19381 agaaaatggg tgagcatcct tcccgtcagc gaatagggat ccagtagaat gaccagcgac
    19441 agggacccga aaagcgccgt cgccgccgtc aaagccagaa gaccatagcg caaaacgttc
    19501 aggggctttg cataagaggg cgcggggcga aggccgagtc ttttcgatcc ggccatcagc
    19561 aaatcctgca gcgtgcccag cggacacaaa aaagaacagt acacccggcc gaaaagcatc
    19621 gtgatcagaa ggatgaaggc caggcccgca agaaaaacga cgtcgggcct ggtgagggtt
    19681 cgcagcaaag ccggcacgaa ttgcaaaggc ggcaggattc cggaaaggac ggcggacatt
    19741 ttttccgcgc ctgcgaacag cagaacaaaa aggataaaaa tggcgaaaga caggattctg
    19801 cgtgttttaa tcagtgtcaa tgagggtttc cccttgtttc gatcctgaaa aagttgccgg
    19861 tgttgccggt ctttgagcgg cggattcgcc gcccggatgg cttgtaaggc ttgagcacgg
    19921 tcggccgcaa cggttctcta catcgcgtag gtggcgattt tcagctcttt caagttcatg
    19981 ttcccgattt tctgcgcgtg ggcgattttg atatagccga tggactccgg ctctctgccg
    20041 aagattttgg cggcggccgc gtcgatggcc acgatgtcgc gcgagatcag cagggtcttt
    20101 ttcatctgga tatcggcgga ttgcggattg ccggggcctg aaggtccatg gctcatcagc
    20161 acgcggtaag catccaccac gttgaggtcc ggtttgcggt acagacagaa atccgcaatg
    20221 cactggtcca gtccggtcag atggtacgcc atgcgatttt tgacgacgcc catcaggttt
    20281 ttcatggcga tggtcaggtg cgtggagctg tggtgtttga ggaccggcac attgatgaaa
    20341 acatcgcaat ccaggatcag actgtgcacg tccgcggttt tgaggatttt gcctccggga
    20401 atccggactt cgcgatagct gaaattatcc acggggacca cgacggcgcg ggcggctttg
    20461 gcggcttctt cgatgccgct cagttgatag cagttgcggg cgtttgacac agtgggcgag
    20521 accacgttgt cgaaaacgta aacgcgcctc gcgccggccg cccagcagga ttcgacgatt
    20581 gttttgacga gcagtggatt ggtattggcc ccgacttccg gcacccgcgg ccagccgatg
    20641 ttgggtttga caaccaccgt ctgccccttc ttcacaaact gctccatgcc gcccatcagt
    20701 tcgatcgctt ttttgaacat ggcatccggc tcgccgtttt tgacggcaac cagatccggg
    20761 atgcgggcgg atttttcttt ggcccagagg aaatcaggct tgcccggaat caacgcgccg
    20821 cccgcgacca gacccaacga aagacccctg aaaagaattt cccgccgttt gaatttttgt
    20881 tcccccgcca tggttgcctc ctgccggttt ttgacccggc ggctatcggt ttttcaatcc
    20941 ggcgatttca gccagcagac gcgaacgatc gaaatagacc tcatttcgac gaatttttcc
    21001 cgcatcgtcc aactgaacaa aacagacgcc gacgatttcc agcgtcgcgc ttcccacggg
    21061 gattctggcc agccatttgt ttaaaaaacc gtcttccagc gggatgcctt ccgtctgccg
    21121 ccaaacccag ttggggttga ccgccagcag tttgcgaaaa taagccagca aatccttctt
    21181 gccttttaca ccatggggaa tgcccggatc caggtaaaag gcgtcgtcgg tataaaaatc
    21241 cgccagacgt tcgggatggt tgccggacca ggccggcagc cattgttcgg caaattttct
    21301 ggcttcctca gcggagagat atttcatatg gcctccatct ttgtgatgaa acacctgttg
    21361 tgtcgccttc ggcatttctg tgttcgctct tgccttggat tcttacttaa aaaccgtctt
    21421 ttcgtaaaga ataacttttt ccatcgccct ttcaaaggaa tcatgatttt tatgttataa
    21481 ttcctttgac acaaaagcgg caatctctta tacttcgccg caaattctaa cgtattctta
    21541 aaattgattg gggtcatatg accgtcattg cgctgatttt attttatctg tttttccccg
    21601 tgttggccgt ttacctggcc aggaaaaacg ctctctgtaa caaactcggc gccgtcgtga
    21661 tctgttacat ttgcggaatc gtgatcggca atatcggcgt gcttcccgcg aatctggctc
    21721 ccattcaaaa tcccctgatg ctggttgcca tcagtctggc tctgccgctg atgttttttt
    21781 ccattgacgt gcgtcgctgg agccggctgg ccggcaagtc gctcctttct ttcggtttgc
    21841 agacgctggc gatcctgatc gtggccgctc tgggcttttt catcttccgc ggccgggtgg
    21901 gggaagaaac ctggaaactc gccggcatgt ttgtggggct ttatacgggc ggcacggtga
    21961 acctgggcgc catcgccacg gcactgcgca tcgattcaac tctgtacctg gccgctcaca
    22021 catccgacgt tgtcgtgtcc gcggtctatt tgctttttct gatgacggtc gggcggcgga
    22081 ttttgcttaa atttttaccc gctttccaaa cggccggagt tgtgtcgcct gccgatcatc
    22141 cggatttcga ttcttacgcc ggcatttttg aaaagaaaac ttttgttcca ctcctgggcg
    22201 ccctgggact ttccgtgctt atttttataa cgggaggctt gttttttctt gtgtggcatg
    22261 aggatatcgc ttttgtcgcg gccatccttt ccatatgcac gctggggatc gcttgttcgt
    22321 ttgttccccg aatccggaat ataaagatga cctatcaggc aggcaattat ttcattctga
    22381 tcttctgtct tgtggtgagc tccatggcgg actttaaccg ccttgtgtcc actgccccgg
    22441 ttatgctggt ttatgtcact ttcaccatat tgatctgcct tgcgctgcat gtggcgctgg
    22501 cgagaatatt caggatcgac gcggacaccg tcatcgtgac ctctgttgcg ggaatttgct
    22561 cgcctccgct cgttccgatg gtcgcttcgg ctttgaagaa taaggaaatc gtactttccg
    22621 gggttatcac cggcatcatc ggctgggtca tcggaaccta cctcggaata tcgttgtcgt
    22681 atattctgcg cggaacaggc gcttaatcga tcaatttcat taggggaggc tgtcatgaag
    22741 ctttatgaat ttaatgaatc gtaccggttg ttcgaggaag cgcagcagta cgttcccaac
    22801 ggcatttacg gtccccgcac tccggccttt ctcaccttcg ggtcatatcc cgcctttatc
    22861 cggcggggag aaggatgccg gatctgggat gtggacggta acgaatacat cgattacatg
    22921 tgctccttcg gaaccaacct cctgggattg aagcatccga aggtcgatgc ggcggcgaaa
    22981 aaacaatggg aaaacgcaga ctgcttcacg ctgccgtccg atctgtggct tccgctcgcc
    23041 aaaaaaatcg tgggaacgat tcatggcgcg gactggtgtg ttttcggaaa aaacggctcc
    23101 gatgtgacgt cgtatgcgat cacgctggcc cggatattca ccggaaagca ggacatcctg
    23161 ctggcgcacg ggtcctacca cggctcccac ttctggtgtc agccccacgg gcagggggtt
    23221 ccggaagaat ggaagacgca tgtccattat tttgaataca acagcgtgga ggatctggag
    23281 cgggtcgttt ccgagcacga aggcgccgtc gccggaatca tgctgacgcc gcaccggcac
    23341 gattccatga gggaccagga gctgccggca aaggaattcg tggccgccgt caaccgactg
    23401 gcgcaaaagc accggttcat gatcattgtc gacgatgtcc gctgcggttt tcgccttgaa
    23461 ctggcgggaa gctccattca ttacggattc aacgccgata tccaatgctt cggcaaggcc
    23521 atggcgaacg gatatgccat ttccgtggcg gcagctcgga aagaaatgat ggatgcggca
    23581 aaaaatattt tctttaccgg tacacattac ttttcaggcg ttccctttgc ggcggcgctg
    23641 gcctgcatcg acgagattca ggccgcaggc gccatcgagt acattcgcaa gacggggact
    23701 ctgctcatgg aaggcttgag gcaggccgca aacgcggcgg gcctgcaggt caatgtcacc
    23761 ggtcccccgg cgatgcccta catgacgttt gccgacgatc ccctctttga gaaaaaccgg
    23821 tttttctgcg gtgaggcggc gcggcgaggc atctttttcc atccgcacca caactggttt
    23881 gtctgcgccg ccatgacgga atcggatatt ctcaggacga ttgatgtggc ctccgagtgc
    23941 ttcaaactga ccaaagagaa atttgggggc tgatatgaaa tggtatcatg tcgggatcaa
    24001 agccgccgcg agcgaggcgt ccgtccaatt ttaccgggac attctgggac tggaagtgat
    24061 cgaccgggtg gaaatctggg gaaagcaatt tgtttttgtg ggaaacgatg ctatttccat
    24121 tgagattgag caggccaatc ccggggatac ccaatcggat ccttcttcca tgaccggctt
    24181 gaatcatttc agcctgattg ttgaggatat tcgggaatta gtgagccagg cccgcgaaaa
    24241 gggcgcctgc gtcctgatgg atccgtttca tccccggccg gaccgactca cgacctttct
    24301 ccgggatccc gacggcgtga tgattcagct gattcagttc gtttaaatga ttttgatatc
    24361 gaagcgttgc ggcgccgcag gcagtgatga atgcgtccac tcacgcgctc aagaatggtt
    24421 aacagggaaa gcatttcccg gttgcccgca atttcggtga taaatataaa tgaaaaatta
    24481 tattgacatc acttaagtga ttgattataa aaacattgca cataatgcgt cagagaattc
    24541 ataagccttt tattttttag tggaatcagg gcggtttggc ttgatttaac aaagattgat
    24601 taaacgtaaa gattacggtt attttaagga gagaaattat gaaaaaattt gcttcattgg
    24661 ttgttgtcct gctggtggcg ttgctggttt ctacaggcaa cgcgcaggtg agggcggggt
    24721 cttttacgat cacgccgact ctgggtttgt acacgtttga agggaacgaa gacatcgatc
    24781 aggcggggcc cttggtcggg ctgcgtctgg gatacaactt caacaagtac atcggcatcg
    24841 aaggatacgt tcattattcc caaacagaaa acaatgcctg gtctcccaaa aagaatgttg
    24901 attttacggg gtacggcggc gagattattt tccatctttt gccgaatcag gcgctggtgc
    24961 ctttcctggc ggttggtgct ggaggcgtcc actattccta tggggtattt gaggacgatt
    25021 cggattacgg gacggacaat tacaaagaca acaagtttac ctttgattac gggtacggtc
    25081 tgaagtggtt cctgacggat tggttcgccc ttcgtctgga tattcgccac gtcatgccga
    25141 tcgaccggtt tcacaacaat atgctcgcgt ccctcgggtt gaatttcgct ttcggcggcg
    25201 ccaagaaaac gacggcgacg gctgtttccg cggccgagcc ggtcgtggag gaggcggcca
    25261 agccccaggc tgcggccgct ccggtcgttg aagaatccag acctgctgtt gcggcggcgg
    25321 ctcccgtggt ggttgaagaa gtcgtgaagc cccagcccaa gacccaggtg cgcgaagaag
    25381 tcgatgagat gggaagagcg acgctggaag tcttgtttga tttcgacaag gccaccatca
    25441 aaaagaatta caccaaggat atcgatcata tggtcgatgt gatgaaagaa cattcggacc
    25501 tcgacctgac catcgaaggc catacggaca acgtgggcaa gcccgcttac aacaaaagtc
    25561 tttctcaaaa gcgtgcggac tccgtgaaga actacatggt tgaaaaaggc ggcatcgatg
    25621 ccaagcggct gaaagccgtc ggctacggcc aggaaaaacc cgttgcttcc aacaagacca
    25681 aagcggggcg ggcgaagaat cgccgcgtgg aagcggccgt cgattataaa aaataaataa
    25741 gaaagccttc cgtttaaaag gctttcagat caaatggagc ccccgcggtt ttttcggcgg
    25801 gggttttttt tgttgcgcgc gtggcggggc ttatcccctg gcggttgaag tccgccgaga
    25861 gccctttgac gaattgagag ttttgaataa cagtcatttt tatcatttcg accgaaggga
    25921 gaaatccatg tcccgaatgc aatgagggat ctttagattt ctcgtcgctc acgctcctcg
    25981 aaatgacctt tttcaaagct ctcgaattgt cccgcttgac cgggggcatg gagaaccttg
    26041 attaatcatg gtaaatgtat taaatcttca cacgggtttt acagggccgc ttccatgccc
    26101 gcccgtggat gaaaaagaca accgatccag tccgacccgc aagaagggtg aaaggagcaa
    26161 atatgaaaaa gatttatgcg gtggtttata tttttattct ggcccttttt ctgctggcgg
    26221 gctgtgcgaa agctccgcct gttgtcaaaa aagatgagcc gaaagtcaaa gagcctcccc
    26281 cggttccggt cgtggtgaaa aaacctcctg ttgctccgcc tcctccgccc cccgacatgg
    26341 aaaaactacc cgacattgtg cgcagcgtac cggacagcaa ggtgtgggtg cgctatggaa
    26401 aaaccaaaat gggagagaac tattacaacc aaaccaatat cgccagatcc tccaatatcg
    26461 cgaccgtcac cctttatcgg gttgtgacgg atctttgcag gaaacagatg attgaagaag
    26521 tgaagaaata caatccgaag aaatcggtaa aataccatta ttacgaacat gatgtccgcg
    26581 tggatgagat ggattgcaag aatcaaaaat ggcgggtgaa agacctgatc cattacgacg
    26641 acaagggaag cgttttggat catcatacct acgaaaatga accctggcaa cccattaaat
    26701 ttgtaacgga tcatcacacg ctgcagaaaa gcatttgcgt ggccgaggaa aagcctgtcg
    26761 tcaagaagaa aaaaaagaag tgaggtgagg atcgtcaacc gttttcagaa ggtttgcgcg
    26821 ccggcccttg cgtcttcgcc ccaacggttt ttttccgaag cagggcgcct ttcggcgccg
    26881 ggaagggaag ggggacgcgg atgaggtttc ccgcgtgagc ccggtcgatt ctgtttcctt
    26941 catcatccgt catcggtcgg gcatcgacgg gcagggtttc cttcgtcagc agcaattcca
    27001 catcctcgcc cgcctgcagg aaattgcgga cctccatccg tagctccttg gtgacgttgt
    27061 catagccgac aaccattccc gccggttcat gcgtctgaat atttttgatg tccgggctgg
    27121 attcattgat cttttcttcg gcaaaggcaa agcccgtggt gtagccccga tgggagacgg
    27181 ttttcagctc ctccatccaa ttcgggtcgc agcgataggt ctcctggtga aggcaaaggg
    27241 cgtccagcgc ctgccgatag gttcgggtca cgaccgccac ataataggaa gacttcatcc
    27301 ttccctcaat cttgacgccg gcgacgcccg acgccaggac ctccggcaga tgttccagca
    27361 gacataaatc tttggagctc agcagatacg aaaaccggtc atcttcttcc aggacgagag
    27421 gatcatccgg gcgggtggat tccgaaagac ggtactccca gcggcagggt tgggtgcagt
    27481 cccccgaatt ggcgcttttg cggtttcgcc aggccgagag ataacagcgt ccggaatacg
    27541 ccatgcacat cgcgccatgg ataaagatct ccagctccat gccgggaacc gccgcggcga
    27601 tttcccccac ttcgcataga ggcagctccc gcgcgagaat aatccggctc accccctggt
    27661 cctgccagaa gcggaccgct tcggcattgg tggtgttgga ttgtgtgctg agatgcagcg
    27721 gaacctgcgg aaccctcctg cgggccagcc ggacaagccc cggatcgctg acgatcagcg
    27781 cgtccactcc cgttgcggcc cagtcgggaa gacaatacgc cgcctggtcc agatcggcat
    27841 tgcgggcaaa cgtgttgatg gcgccataca cccggacgct ccgggcgtgc gcttcggaaa
    27901 cgcccagggc cagatcctcc agggacattt cggcggaggt tccccgcagg ctcaaaccgg
    27961 gaacgccgac atagacggca tcggctccgt aaagcagggc ggtgcgcaat ttctccaggt
    28021 tgccggcggg ggcaagcaat tccggctgtt gcatcgaaac ctccgtcagg gacaatacct
    28081 gcgcacctgc tccaggactt tgagagattt gatttctttt cccagtacat gggaaataaa
    28141 gccgcagagg atgctgcggg attcggaggc aaggctttcc gtcatgcaga ccattttcat
    28201 tttatccaga tccatgtctt tccccaggag cagcgtacgg acggcgccgg cggagacgct
    28261 ctggtcgtat ctttccggcc gggcgcaggc cgcgcagaaa atgccgcctt cattcggtaa
    28321 aaagatgtag gcggcgccgt tggtcacggg cgttttacag cgcacacagg ccgtaagcgc
    28381 aggttcaaat ccgacggcct tcaacagccg catttcaaag aaacggatca tggactccga
    28441 aacatcttcc cgggtcagaa gcgacaaaaa atccgccagc aatacgaaga ctttgtcgtt
    28501 gcgcttgcct tcggggctga agtgttcggt cagatcgatg aaataggaag cggtcagggt
    28561 cttttccaga tccaggcgaa tcggggcaaa gtgttcaagg atgtcgcagg aatcgatgaa
    28621 cgcgagcgcg tcgcggttgc ggcgtgaaaa aatcatctgc gtcagggaaa agggttcaaa
    28681 aacgttggcg aaccgccttc ggctccgttt ggcgcctttg gcaatgccct tgagcttgcc
    28741 gaactcacgg gtgtaaaatg tgacgatcag atccgattcc ccgtattgaa ggcttcgcag
    28801 gacaaaaccg gcggttttat gggtttcccg ggcgctcacg gtttggatgc cagtccatcg
    28861 tacttgatgt cgccgcccac gatggttaaa acggcgcgcc ctttcagttt tcttccggaa
    28921 aacggcgagt tttttcccag cgaatggaag gcggcggcat cgacgctcca ggcaagctcc
    28981 ggatggataa ccgtaatgtc cgcgtcggcg cccttggcca gcgtcccttt gggcagattc
    29041 aaaatgcgcg ccgggttgca gctcattttg gcgatcagtt ccggccacga cagaacgccc
    29101 tcatgcacca ggctcaggct caggcccaga gaggtttcca gaccgctgat gccattggcg
    29161 gcatattcga actccacttc cttgtccgtg cgtccatgcg gcgcgtgatc gcaggcgatg
    29221 gcgtccagcg ttccgtccgc gaggccctct ttgacggccg aaacgtcggt cttgccccgc
    29281 agcggcggac tgaccttgta gttcgtgtcg taatctcgaa gcgattcatc cgtcagcgta
    29341 aaataatgcg gggcggtttc cgccgtcact tgcaggcccc gttttttggc gtcgcggatc
    29401 aggcgcacgg agccgaccgt gctcacatgc gcgaagtgga tgcgcgtgcc ggtgtactcg
    29461 gcgatcagaa tgtcgcgcgc caccatgatc tcttcggcga tggcgggaat tcccggcagc
    29521 cccagaagcg tggaggggta tccttcattc atcaggccgc ccgcggacag atttttgtct
    29581 tcgcagtgct gaatcaccgg caggttcagg gaatcagcgt attccatggc tctgcgcatc
    29641 agggccgcgt ccatcacggg attgccgtcg tccgacaaag cgacgatgcc cgcctccctg
    29701 aggtcccaga attcggtcat ctgtttgccg tcggagttca tgctgatcgc tccgatggga
    29761 tagatattgg ccagattcgc ttccgcggcc tttcgcagga tgaattcagt gaccgagcgg
    29821 ttgtcgttca ccggccgggt gttgggcatg caggcaatcg acgtgaagcc tccggccacc
    29881 gcggcggcgg cgccgctggc gacggtttcc cggtattcat atcccggttc gcgaagatgc
    29941 gtgtgcatgt cgatgagt
//
LOCUS       DAOSRM010000013        29149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1763_length_29149_cov_111.362515, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000013 DAOSRM010000000
VERSION     DAOSRM010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 29149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29149
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1763_length_29149_cov_111.362515"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLA58_02630"
     CDS             <1..674
                     /locus_tag="PLA58_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61721.1"
                     /translation="AAVALVLQAADHGFEGGNMPKNTILHALVFIQNWGEEDGLMNFL
                     RGNPHAVSIFHVMGRHSYVVDSNFDDKEQLHDWINRLKTIRLSAGVPAVISVQTQKVI
                     QVFKQKNDFDLKDYRDLADRYHFFMLIDNPHHDEKLIELLKKLRIVSSILHTQGANSF
                     IVEIITEAYDSYKKMLGKAKELKSIQHIETLEVLSVKKYRNQIIDKGGELSYPRTDLR
                     ELYTL"
     gene            complement(731..1513)
                     /locus_tag="PLA58_02635"
     CDS             complement(731..1513)
                     /locus_tag="PLA58_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOR61722.1"
                     /translation="MAFEYEKLVVEQDGAVVVAAINHPPANAMAQGVLRDLNDLLDKC
                     EKDDGVRVIIITGTGPKCFSAGADITEFADLQSGKVPQYDGNAVYFKIENYPKIIIAA
                     IQGLALGGGLELACSCHLRIMAEEAVVGLPEVKLGLNPGWGGTQRLPRYIGKTKALEL
                     MLTGDFMPANEAYARGLLNKVVPQAEVLPAAKKLAKKLAGGAPVAQREIMKAVRLGLE
                     GTLYDGITKIEKAAFQTLLVTEDFKEGSKAFLEKRPAQFKGR"
     gene            1798..3555
                     /locus_tag="PLA58_02640"
     CDS             1798..3555
                     /locus_tag="PLA58_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOR61723.1"
                     /translation="MNVAVVLIIAVVLFFLAYRFYARFIAKLFDENDNRPTPACALRD
                     DRDYVPTKPVVLFGHHFAGIAGGGPIIGPTVALLFGYLPVWLWAVIGTIFIGAVQDMT
                     VLFASVREKGKSIAEIAKESLGNTGFFLFISFAILMLLLLTSAFLGLTAVSLTSLVPL
                     EMMRLAPDQTIVKTVLVGGVPNAQIGGISSTSLIIITAFAPVIGWLVYKRGVSVNVAV
                     PLAILVCIGSILLGMKYPVSLDPDIWMIILCFYTLLAAGIPVWIILQPRDFTNSFLLY
                     MGVAALFAGGIAAGFQGVTFTAPAFNLAQGASKLGPVWPFLFITVACGAISGFHALVA
                     GGTSSKQVAKESHVKKIAFGGMLLEGLLAIGVMIAVGCGILFSDYVNIAFPTAAGVKA
                     NPILAFAIGVGGLLDKALGIPPVYGTVFGILMVEGFIITTLDTAIRLERYLLEELWQV
                     LFKNVPKIMKSYFFNAFLCVVLMYILAYTNAFAAIWPIFGSANQLLASLALIAVSAWL
                     AKRKKTAWFTVLPAIFMMITTLYSLVSLLVHKYLPKSNYALAVTDVALMILSIGVIVL
                     ALRSWRELRNGPSTAGEAV"
     gene            complement(3564..4148)
                     /locus_tag="PLA58_02645"
     CDS             complement(3564..4148)
                     /locus_tag="PLA58_02645"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOR61724.1"
                     /translation="MDIAAFYHSEIFKFVLLPVLIFLARICDVTLDTLRIIYVGRGMK
                     FLAPVIGFVEVMIWLVAITQIFKNLSSPVCYVAYAGGFAMGNLIGILIEEKMAIGTVV
                     IRIITQKDAETLIRCLKENRYGVTYVDAQGAMGQVKIIFTIVKRKNIDSVLGIVRDCN
                     PLAFYTIEDVRSVRKGVFPLFKAAGKGTGPAADG"
     gene            4535..5377
                     /locus_tag="PLA58_02650"
     CDS             4535..5377
                     /locus_tag="PLA58_02650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR61725.1"
                     /translation="MALSLAAMPFESRSSGPDVSFSSDAMVRGGLILLRVQGCESDDV
                     QVVWMGKEIPLVRDREGTLRHGFLAADLNREPGVYPVAVRDPSSGFEKYFELRVNDRD
                     YGVRKIRLPRAKVEPDEASLRRVKAEALAVNALWKAECRPPEWEGSFWMPVNNEIVGA
                     FGQRSIINKKKRSPHTGVDIRGKTGAPVRVSNSGTVVLAADHFFTGKSVYVDHGSCIF
                     SMYFHLDQIRVEEGEQIEKGGILGSVGATGRAAGPHLHWGVQINGARVNPLMLIELSR
                     ELDP"
     gene            5540..5950
                     /locus_tag="PLA58_02655"
     CDS             5540..5950
                     /locus_tag="PLA58_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOR61726.1"
                     /translation="MRDFRNILYAMDLDAEHFSSLVRALEFARRFQSRIHILYVNDPQ
                     AGYRHPADHEDAVALKVKEAAPESLLENLKVVYAVSKGDTAGEIVRYAGENQIDLIVV
                     GHKHRGKLYASMFDSTDVHVIDQAMLPVLVFPET"
     gene            6013..6804
                     /locus_tag="PLA58_02660"
     CDS             6013..6804
                     /locus_tag="PLA58_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOR61727.1"
                     /translation="MKIKKDAKQKRIRIGLFGFGKTGRLVAEEFFQDGLFDLEWVVRK
                     THKENHKYATRLLGHEVDDASVFSVDEVTPEFYRRHPVDIIVDFSSAAAHRHYAPAAD
                     FGIKIISAISKYRTEDVEDLRRMAEKTAVLYSPNITLGINFLLVASQILQGIAPHADI
                     EIVEEHFRGKKGESGTALKIAEILGLEKEKRVNSIRVGGIVGKHEIIFGLPNQTIRLV
                     HESISRAAFGQGAIFAAKWLIHAPPGLYSMENIIVDMFKKNIPVY"
     gene            complement(6801..8729)
                     /locus_tag="PLA58_02665"
     CDS             complement(6801..8729)
                     /locus_tag="PLA58_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HOR61728.1"
                     /translation="MKILPEGRITPRDLQSAGAKAVLEKMRRMPGYYLTNTERDLSQS
                     DFKNRILPHTQMLVAPERNEAGYFSLEITGGASVHVDMLRRQMNPLEKLEVLAGHMPD
                     TLFQTLCRGINLFGYRPYPENVIRLTVETFARRVHVWRVFDFLNYIPNMVPVYEEVRK
                     AGRLLEAAVCFSTGPEHTNRFYIRKVGEILDVTGPDILLAIKNHGGLGSPGRIGELVR
                     AIREKYPDLLIHYHGHNTDGNEIGRIVAAVRNGAKIVDASDHALTGFYGPPPLLTVVN
                     TLEDYGYNAVGLDKQAVIEASNKLRHERPYYRDFESQFFGFDPTVQTHKLPGGATGSS
                     FEQAQKGGFLDRMPEILQNELPRVQVELGNFWSVTPGSQILWTTAANNVVKGARYRDA
                     SDDLKNLMLERYGKFPFYKPAPEIYEAVFGKDWKKIVRREAGYQKIEDVDLDVERRIL
                     EHAIGRKATDDELVLYLQHPNDAVGFFKFEEKFGKTWVLPPKVWFRRGGFGIGDKFEF
                     PDASGKLHTIEIGPSRRTKDGSYMTHLGVDYHPEPVVTPADDEGEKPKASVVLSAKEI
                     GALARMGDIRATLKGVVNQIPVAVGDEVAVGDTLVVLEAMKMLTNITADVDGKVSEIF
                     ASPGASVEIGDPLMMMEL"
     gene            complement(8759..10207)
                     /locus_tag="PLA58_02670"
     CDS             complement(8759..10207)
                     /locus_tag="PLA58_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOR61729.1"
                     /translation="MKKIRKVLVANRGEIALRIIRTVKEMDLDTVAVYEKPDREAYFL
                     RFADQAILMGDGPRRDYLNIDKIIWAAQKSGSDAIHPGYGFLSENPDLAQACVDAGIS
                     FIGPPPQVIRDLGNKVVARNIMEKAAIPFVPGTGTLSAGDAGLTEAVAFGKTHGYPIM
                     LKALAGGGGRGIRKIDSEEELRSQLPQARSEARSAFNDDRIYIEKCVESPRHVEVQIL
                     ADAYGHVMHLGTRDCSIQRRHQKLLEIAPADLPKNVLDAMHQTAIRAAREAGYVNAGT
                     VEFLVDPRTNRFWFMEVNTRLQVEHTVTEILTGIDIIRQQILMAEGETLDIPEERIHL
                     VGKAIQVRVNAEDPKNNFMPEGGKRVEVYQSPGGPEIRLDGIVYQGYKIPTDYDSLLV
                     KLTVRGYSWQQTIQRLKRALKDFLIVGPKTTIPFYLAICDDKDFRDGRFTTDYLEAHP
                     ELFDYPEPEREIAKLSKLIAEIHARKINPYAY"
     gene            10363..12261
                     /locus_tag="PLA58_02675"
     CDS             10363..12261
                     /locus_tag="PLA58_02675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR61730.1"
                     /translation="MKDCTMAGFFDYRSKVIGTDQVLEKLKPGQKIFLSSGVAAPGKV
                     LTALARSDAKNLRDLEIIQLITLRKYLSHQSSDQYRLKTFNVGESISKEVAEGRVDFI
                     PANLMELPYIFLSGAVAVDVAIIQTSVPDDKGFMSLGVAVDVANIVIKQAALVIAEIN
                     PNMPVTLGETTIHISSIDYVVESDLPLVERINRPYDEITDRIGWNISNLIDDGATVVL
                     HVGRLFNAVADHLKQKRNLGIVTHVVSDWAVDLIESGAISLDRSRFNGGLITASYCYG
                     SKALYRYVHRNPIFEFYPIARLNNPFVVRRIKSLIGIMNVKKIDISGESVIFHSGDNL
                     LTGYESKLNFAVGATMTRRGKAIVALNSVDTEGNSNIVIKFDGRTDNTHSTLGVVKYI
                     VTEYGVANLFGKSIRERALAMIDIAHPDHREDLLRQAKALNYVYADQIYVARHAANYP
                     ALLETRKSLKDSLEIKVRPIKPSDEDKMRRLFYNFSDESKYFRYFAPKPVMPHKEMQT
                     YVSIDYDKVLSVVAVAEKGRNERIIAEARYAYDKSAGAYEIAFIVDEDFQGRGVATFL
                     FKYLIKIARDRGIQWFIAYVLPRNESMMKVFEKSKLPIQKSYENDALVLRFDLTQEVA
                     DTDAAARK"
     gene            12420..13709
                     /locus_tag="PLA58_02680"
     CDS             12420..13709
                     /locus_tag="PLA58_02680"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOR61731.1"
                     /translation="MSNAVIVSGVRTAVGAFGGSLKDVPAKDLGALVIRESLIKAGFK
                     PVLPAHAKEDAPDTAKNEGLCPVEQQYSRWDANLREIAVDEVIMGNVIGAGQGQNVGR
                     QAMIRAGLPKETTAITVNKVCASGMKAIALAAATIKAGDNEVVIAGGMENMSDVPIGL
                     PKARWGHRMDMPFGKTVDLMVFDALYEIFYGYHMGVTAENIAARYNISREEQDKLSLE
                     SHTRARNAIKSGLLKDEIVPVTIPQKKGNPIVFDTDERPMETSMEKLAKLGTAFKKDG
                     TVTAGNASGINDAAVAMLIMSEDKAKALGVKPLARIKGYASGGVDPAYMGLGPVPAVR
                     KLLRITGTSVKDYGLFELNEAFASQAISCLRELNIPWDITNVNGSAISIGHPVGCSGA
                     RIVLALANEMKRRNVGLGLASLCIGGGQGMAIAIEAV"
     gene            13772..14572
                     /locus_tag="PLA58_02685"
     CDS             13772..14572
                     /locus_tag="PLA58_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR61732.1"
                     /translation="MPYQTVNGIKIYYEEKGTGEPLLLINGLAFPMDLWFAQIEELSK
                     DFRVIALDNRGIGRSDKPDDVYSMSLMASDAVGLLRSLGIQKANVAGLSMGGFISQEI
                     ALSCPEMVNRLILVATGMGGPRSLALGKPFWDRVAAAIAGKNPWEIYRIDLTLMTAPG
                     FVEEHPETLDKAVELRMKNPQPFHAFVRQFTAAGLFDSNERVQKIEHPTLIVLGREDP
                     IFPIALAEDFREKMPHAKMIIYEQCGHAILLEKPDRLSKDIREFLKGN"
     gene            14687..15997
                     /locus_tag="PLA58_02690"
     CDS             14687..15997
                     /locus_tag="PLA58_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOR61733.1"
                     /translation="MGIKNIKTVSPDQAVRAVRSGDHVHISATSNTPACLVAALCRRG
                     RSGELRGVHIHHLHTEGPAPYADPSFEGVFQLESFFVGPNVRLSTQQGYADYIPVFLG
                     ETQRLYREGYLPCDVALIQVSPPDEQGRVSLGTSVDASVAAVEKAGVVIAVINPHVPH
                     TYGDGLIPTDRIHVFVEDDTPLETVHFAPPGDVEAAIGEHCASLIEDGATLQMGIGAI
                     PNAVLAQLSGHKNLGIHTEMFADGVIDLVEKGVINGANKAIDRGQMVATFLLGSKRLY
                     DYTHRNDRVLMKDVGYTNDPFVIAKNPKATAINSALQVDLTGQICADSLGTKCYSGVG
                     GQIDFFYGASRSAGGKPILAMPSVTEKGESKIAPILLPGAGVVTTRAHAHWLVTEFGA
                     VNLYGRSLQERARLIIGIAHPDHREALDRAAFERFGPHYHFARI"
     gene            16161..17999
                     /locus_tag="PLA58_02695"
     CDS             16161..17999
                     /locus_tag="PLA58_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR61734.1"
                     /translation="MKDHAKTKQPMIGELASLRERIAELERSALEQKRVEEELRESAE
                     KYRLFFEYSPLGNFYFDEKGVIVACNEHFVDLIGSSRKALIGLELFKLPDQKVVEAVR
                     QALGGQTALYEDVYRSVTADKATPVRAVFTPIHLEGRGVIGGVGIVEDITERRRSEAA
                     LREKEERYRSLVENADEAILVIQDGKIQFVNTRAVAAFGYSVQEFISIPVFELVHPED
                     REMVIRRYLEKISGDAAPTRHTYRVLPKKGLVSWIEISSILIHWEGRPATLNLITDIT
                     DRKKAEDALREREEKYRTILESMEDGYFEQDLAGNFIFVNDSMSRIYGYPKEELIGMN
                     YKKHTDAESGRKCFHIYLNIYKTGKPGKVVDFDIIRKDGARRHVESSVSLIRDNAGTP
                     IAFRGIVRDVTERKQAEEALNQSFARLRAALTATVGATAMMVETRDPYTAGHQRRVAD
                     LGRAIAEEMQLTDEQIEGVHMAGMVHDLGKISVPSEILSKPTRLTDLECRLIKTHPQA
                     GYEILKDIAFPWPIARIVLEHHERMDGSGYPHGLKGDQALLESRILSVADVVEAMASH
                     RPYRAGLGIEAALAEIEANRGILYDLFVVDACLKLFREKGYRFPES"
     gene            complement(18085..18567)
                     /locus_tag="PLA58_02700"
     CDS             complement(18085..18567)
                     /locus_tag="PLA58_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61735.1"
                     /translation="MSDRIGNFIKSVSACLLMTMVGCSGYGILQKQEVSGIPDASYER
                     IIKLENPEGAPDKTLAGLVHVTGKETICADFPRQRVIQSLDDLEKPEKQALAHFQNYA
                     IRDGDETLGYVSVPVEYGVNIWRRDNDPVCKYSVQILPPRHRHGQTDYQDAVQGSTSW
                     "
     gene            18796..19299
                     /locus_tag="PLA58_02705"
     CDS             18796..19299
                     /locus_tag="PLA58_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61736.1"
                     /translation="MKVFKIKFGKSGEKADAFGIRFLGDFDGKMGQNGSFSEIISPPD
                     SAPLITHQRITIWFTFERIRPYDTVSGFVRELSELLKEKDYIIKYSFIDGLFNTTLLD
                     DANPPEGFSDPRDPGNAATGFSVTAEKHSAKLKFTVNEIVSVSEAAAKLGRTIYGRNL
                     ERVRVNR"
     gene            complement(19332..20963)
                     /locus_tag="PLA58_02710"
     CDS             complement(19332..20963)
                     /locus_tag="PLA58_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOR61737.1"
                     /translation="MLIGNYLIKAIQEAGARHVFGIQGDYVLHFYDQLHKSDLTLINT
                     CDEQGAGFAADAYARMSGFGVVCVTYGVGGLKLANSTAQAFAERSPVLVISGAPGMSE
                     RSGDALLHHKVRSFETQRNVFREMTAATAALTDAQSAAAEIDRVIDAVMTTKRPGYLE
                     LPRDMVSRETDVPPKNTPKDKQDTSKVLNEALKEVLSMLRAAKRPIAVAGIDIHRFGL
                     QRLFLDFLERSGLPFVTGILGKGVIAESHPQFVGVYAGAMSPDDVRNMVENADFILAA
                     GLLITDLSTGMFTTGTLNPGKAVVACPDSLCVRHHIYPGLGMADVLKALCAKLSATPP
                     KTKWTPKNAPAPFVPQTGCKVTMESLIACVNSLLDDHTSVIAEPGDPLFGGLDLFIQG
                     ASDFMSPAYYALLGFAVPGAVGVSLAAPDRRVIALVGDGSFQMTGMEISTAARCGLAP
                     IVLVLNNGGYGTFRSMIDGPFNDLCPWQYADLPRVIGFGKGYTATNEEELWRALHAAK
                     NNGDELAVIDVKLDKYDISPRLRRLTEQLKKRVIR"
     gene            complement(21407..23632)
                     /locus_tag="PLA58_02715"
     CDS             complement(21407..23632)
                     /locus_tag="PLA58_02715"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOR61738.1"
                     /translation="MEKKTYAPIVPELTKNAITVLERRYLKRDKEGKVLEAPVQMFRR
                     VADTIAAADRKFNEKEDTTAIAEEFYRIMTQLEFLPNSPTLMNAGRELGQLSACFVLP
                     VGDSMEEIFDAVKFTALIHKSGGGTGFSFSRLRPANDVVLTTTGISSGPISFMRVFDV
                     ATETIKQGGTRRGANMGILRVDHPDIMDFIMCKADKKQLNNFNISVGLTEAFMKAVEK
                     DENYDLINPRDNQVSGTLNARKVFNRIVTQAWENGEPGIVFLDRLNRDNPTPHIGQIE
                     STNPCGEQPLLPYESCNLGSINLGKMVQNGKINWKRLGRVVQLAVHFLDNVVEVNNYP
                     LPKIHEMTMGNRKIGLGVMGWADMLIALGIPYNTEEAIELAHKIMGFINEQGHAASRA
                     LAGSRGAFPNFKGSLYDKKGEPPLRNATVTTIAPTGTISIIANASSGVEPLFAVSYVR
                     QVMDNDILLEVHPLFEAMAKEKGFYSPELMQRIAEHGTLHGITEIPEEIRNVFVTAHD
                     ITPEVHIRMQAAFQKYTDNAVSKTVNFAKSASIQDVASVYELAYKLDCKGVTIYRDGS
                     RDHQVLSVGKKEEAEAGAAQGKPATKRERPKVLKGWTYQMQTGCGPLYVTVNEDDTGL
                     FELFTTMGKAGGCAASQSEAIGRMVSLAWRSGLGARQVVKQLQGISCHSPSGFGENKV
                     LSCADAVAKAIQAHVAANGGKVQHVKRVFLKGACPDCGGIIEHEGGCAVCRLCGYSEC
                     A"
     gene            complement(23754..25298)
                     /locus_tag="PLA58_02720"
     CDS             complement(23754..25298)
                     /locus_tag="PLA58_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR61739.1"
                     /translation="MTTAKIIFIHPPVVKPSEPPAGIARLSACLKANSVEHGVIDANL
                     EGMLHLMRAFARTETPVGRWSRRACKNLEENLAALQNPGTFCNQSRYSRAVMDIHRVL
                     HTAGKPFGVQLSLSDYEDGRRSPLRSVDLIQAAEQPEVNPFYSYFSARLAQALEESPD
                     FIGFSMNFLSQALCTMAMIGFVKKIHPRQKILLGGSLATSWIRLSGRTNLFSGLVDRM
                     VEGAGEEKLLALLGMPPRREGPSADYDLLEKNRYFSPGFILPYSAAEGCWWRKCSFCP
                     EKAEANPYRPLSASRAAAELEALARKTQASLIHLLDSSISPALLQALIENPPGSPWYG
                     FARATRHLADEDFCRALKKSGCAMLKLGMESGHQGVLDALSKGIDLAEASAALRALHR
                     AGIAVYGYFLFGTPPETEADAHETLDFIVRHSEYISFLNLAIFNLPAGSEEARSLSTQ
                     KFYEGDLSLYRSFLHPAGWQRGNVRNFIEKTLKKHPAVAPIIRRTPEFFTSNHAPFFA
                     FLKNSK"
     gene            25428..26222
                     /locus_tag="PLA58_02725"
     CDS             25428..26222
                     /locus_tag="PLA58_02725"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOR61740.1"
                     /translation="MKINEPYDLPARLRTLLVLCVFVLIALTSFAAEAADRFPAHKGT
                     AVNDFAGVIDPENAAKMESLSREILEKTGTAIVVVSLAELGPAEEINLYASGLYQAWG
                     IGKKGEDKGVLILLAVKERKIRIETGYGVEGVLPDGRVGAILDQVVAPHLKAGETGKA
                     LYNAMLACGAYLAKDAKVQLTGDFSFYRSAARPEKKGINILGILFFLIAAAVLLGTRT
                     GREMLPWILLMVLSGGGRGGGSGGFGGGFGGFGGGMSGGGGAGRGF"
     gene            26240..26968
                     /locus_tag="PLA58_02730"
     CDS             26240..26968
                     /locus_tag="PLA58_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61741.1"
                     /translation="MARIPNQPQDIFVPLTEDWLNAFGRDLVSLMLYGSAAGGSYVKG
                     KSDINLLVVLTPEGMSRLEDAFAPVKKWRKRNVAVPLVLTQEFISSSLDAYPIEFLNM
                     QNHCVLIYGENVLEPLVFKPEDLRLQIERELKSKIILLREGYLESEGSARRVRELIGR
                     SLVALVSVFNAMLYLKQAGLPRQKKETIEEAGRVFGVDANVFARCFEIKAKEDHLSGE
                     EVIGVFKKYLREVEKVCHRIDGLK"
     gene            26993..27565
                     /locus_tag="PLA58_02735"
     CDS             26993..27565
                     /locus_tag="PLA58_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOR61742.1"
                     /translation="MSAGKKIIIVAAVIAVLGISLYSLFAGNYNKFVQMDVAIKAAWA
                     QVENQLQRRYDLIPNLVETVKGYAKQEKDVLVEVTNARSKVGGAGTVPDKIAANNELS
                     GALNRLMVVVERYPDLKSNQNFMQLQTELAGTENRIAVERMRYNDAVKIYNQAIRTFP
                     ANLIASMFGFKEAAFFEAPREAKAPPQVKF"
     gene            complement(27655..>29149)
                     /locus_tag="PLA58_02740"
     CDS             complement(27655..>29149)
                     /locus_tag="PLA58_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOR61743.1"
                     /translation="PLLISEIFVDSLLGVHVHLFNKKSTHQPDEPNILCQEDLNLKNF
                     QEMYKEKLRTAEEAVRIVKSGDWIDFAMFNGKPVVCDRALAARKEELKDINIMAAVTI
                     PPVPEVVLKDPQGEVFTYNDQHFSLLTRILQQQCKGVFYQPTLYGESELYMRYAQSDP
                     SKLGTNLRDVFFCQVTPMDKDGFFNWGLHNSCCYLQATTAKACILEVNENIPYCYGGN
                     MERIHISEVTYVVEGENTPLAELPEVSPTDTDRKIAENVLSYLKDGDCIQLGIGGMPN
                     ILGKMISETDLKDLGGWTEMLVDAYRDMYESGRMTGKHKKFDTGKINYTFALGNKKLY
                     EWMDHNPAIASCSVGYVNHPTQLCQIDNLVSINQALQVDLYGQVNAESSGFKQVSGNG
                     GMSDFVLGAYWSKGGRSFICLPSTHTNKDGSVISRIVPNFDIGSITTVTRQMVNHVVT
                     EYGCVSLKTCPTWHRAEKLISIAHPDFRDDLIKEAEKRKIWRPSNKK"
BASE COUNT         6250 a         7986 c         8393 g         6520 t
ORIGIN      
        1 aggctgcggt ggcgctggtt ttgcaggcgg ccgatcacgg gttcgaagga ggaaacatgc
       61 cgaaaaacac gatattgcat gcgctggttt ttatacaaaa ttggggcgag gaagacgggt
      121 tgatgaattt tttgagggga aacccgcacg ccgtttccat cttccatgtg atgggccgcc
      181 acagctacgt cgttgactcc aactttgacg ataaagaaca gctccatgac tggatcaacc
      241 ggctcaagac gatccggctt tccgcgggtg tgcccgccgt gatttccgtg cagacgcaga
      301 aggtcattca ggtcttcaaa caaaaaaatg attttgactt aaaagattac agagatctgg
      361 cggaccggta tcactttttc atgctcatcg acaatcccca tcatgacgaa aagctgatcg
      421 aactcttgaa aaaattacga atcgtcagtt ccattctgca cacgcagggc gcgaactcct
      481 tcattgtgga aatcatcacg gaagcctacg acagctacaa aaagatgctc ggcaaggcca
      541 aagagctcaa gagcatccag catattgaaa cgctggaagt cctttccgtc aaaaaatacc
      601 gcaaccagat tattgacaag ggaggggagc tttcctatcc tcggacggat ctccgggagc
      661 tctataccct gtagcgctca aaaaaacggc ggccagaacg gccgccgttt tgcattttcg
      721 cgttttttcg ttatcggcct ttgaattgcg ccggtctctt ttccagaaac gctttggagc
      781 cttctttgaa atcttcggta accagcaggg tctggaaggc ggctttctca atcttggtga
      841 tgccgtcgta aagggtgcct tccaatccca gacggacagc cttcataatc tcacgctgcg
      901 ccacgggagc gccgcccgcc agttttttgg ccagcttttt ggcggctggc agaacttcag
      961 cctgcggaac gaccttgttg agcaatcccc gtgcgtacgc ttcattggcc ggcatgaaat
     1021 cgccggtgag catcagttcc agagccttgg ttttgccgat gtagcgggga agtctctggg
     1081 ttccgcccca gccggggttc aaacccagtt tgacttcggg aagacccacc accgcttctt
     1141 ccgccatgat cctcaaatgg caggagcagg ccagttccag tcctccgccc agggccaggc
     1201 cctgaatggc cgcgatgatg atcttgggat aattttcaat tttgaaatac acggcgttgc
     1261 cgtcgtactg gggcacttta cccgactgga ggtccgcaaa ctcggtaatg tccgcgcccg
     1321 ctgaaaagca tttcgggccg gtgccggtga taatgatgac gcgcacaccg tcgtcttttt
     1381 cgcatttatc caaaaggtcg tttaaatccc gcaaaacccc ctgcgccatt gcgttggccg
     1441 gcggatgatt gatggcggcc accacaaccg cgccgtcttg ttccaccacc agtttttcat
     1501 attcaaaagc catgttccta tcctccttgg gtattgattt taaagatttt catcgtaccg
     1561 tttttaaaaa acgccgaagg cactatagaa agaacgcaaa agcgtgtcaa gaggaaacag
     1621 caaccggccg ccatgcggga cgctgcatgc cggaattttc atttccgcca tgcaaaattt
     1681 tcttgaaacc cggaaaataa ggtgtatgat tccgtcgtcc tgcgggcacg cggggcaacc
     1741 ttcatcaaca ataaatggcg gcgaccatcc aatccgtgcg ctatcgagga gaaggttatg
     1801 aatgttgctg ttgtgttaat catagcggtg gttttgttct ttctggccta tcgcttttat
     1861 gcccgtttta ttgcaaagct ttttgacgaa aatgacaacc ggcccacgcc ggcctgcgcg
     1921 ctcagggacg atcgcgacta tgtgccgaca aagcccgtgg ttttatttgg ccatcatttt
     1981 gccggcatcg ccggaggcgg tccgattatc gggcctaccg tcgccctgct gttcggctat
     2041 ctgcccgtct ggctgtgggc ggttatcggc accatcttca tcggcgccgt ccaggacatg
     2101 accgtgctgt ttgccagcgt tcgggaaaag ggcaagtcca tcgctgagat tgccaaagaa
     2161 tccctgggca acacgggctt ctttttattc atcagctttg ccatcctgat gcttttgctg
     2221 ctgacgtccg cctttctggg actgacggcc gtttccctga cgtcgctggt gccgctggag
     2281 atgatgaggc tcgcgcccga ccagacgatt gtgaaaaccg tcctggtcgg cggcgtgcca
     2341 aacgcgcaaa tcggcggcat ctcctcgaca tcgcttatca ttattaccgc ctttgcgccg
     2401 gtcatcggct ggctggtcta taaacgaggc gtcagtgtca atgtggccgt gccgctggcg
     2461 attctggtct gtatcggctc catattattg ggcatgaaat atcctgtttc cctggacccg
     2521 gatatctgga tgatcattct ctgcttctac acactgcttg ccgccgggat tcccgtctgg
     2581 atcattttgc agccccgcga ttttaccaat tcgtttctac tttatatggg tgtggcggcc
     2641 ctgtttgcgg gcggcattgc cgccggcttc cagggcgtga cctttacggc ccccgccttc
     2701 aatctggctc aaggggcaag caagctgggc cccgtctggc ctttcctgtt catcacggtg
     2761 gcctgcggcg ctatttcggg cttccatgcc ctggtggcgg ggggaacgtc cagcaaacag
     2821 gttgccaaag aatcgcatgt gaaaaagatt gccttcggcg ggatgctgct cgaagggctg
     2881 ctggcaatcg gtgtgatgat tgctgtcggc tgcgggatcc tgttcagcga ttacgtgaat
     2941 attgcttttc ccacggcagc gggcgtaaag gccaacccga ttctggcgtt tgccatcggc
     3001 gtcggcggat tgctggataa agccctcggc atcccgccgg tttacggaac cgttttcggg
     3061 attctgatgg tcgaaggttt tatcattacg acactggaca cggccattcg tctggagcgt
     3121 tatttactcg aagagctctg gcaggtgctt tttaagaatg tcccgaaaat catgaaatcc
     3181 tacttcttca acgcttttct ctgtgtggtt ttaatgtata tcctggccta caccaacgcg
     3241 tttgcggcga tctggccgat tttcggctcc gccaatcagt tgctggcgtc actggcgctg
     3301 attgccgtgt ccgcctggct ggccaaaagg aagaaaacgg cctggttcac cgtgctgccg
     3361 gcgattttca tgatgatcac caccctgtac tcgctggtgt cgctgctggt tcataaatac
     3421 cttcccaaaa gcaactatgc gctggccgtg acggacgtgg cgctgatgat cctttccatc
     3481 ggcgtgattg tgctggcgct gcgcagttgg cgggagttaa gaaacgggcc ttcgacggca
     3541 ggggaagccg tgtgacaaac cttctatccg tctgcggccg gcccggttcc cttgccggcg
     3601 gccttgaaga ggggaaagac ccccttgcgg acggaacgaa catcttcaat cgtgtaaaac
     3661 gccagcggat tgcagtcgcg gacgatcccc agcacggaat cgatgttctt ccttttgacg
     3721 atggtaaaaa taatctttac ctgtcccatg gccccctgag cgtccacgta tgtgacgccg
     3781 tagcggtttt ctttgaggca gcggatgagc gtttccgcgt ctttctgcgt gataatccga
     3841 atgaccacgg tgccgatcgc cattttttct tcaatgagga tgccgatcag gttgcccatc
     3901 gcgaaaccgc ccgcataggc cacatagcag accggactgg acaggttctt gaagatctgc
     3961 gtgatcgcga ccagccagat catcacttcc acgaagccga tgaccggcgc cagaaatttc
     4021 attccccgac cgacatagat gatgcgcagc gtgtcgagcg tgacgtcgca aatccgcgcg
     4081 agaaaaatca ggacaggcag caggacaaat ttaaagattt ccgaatgata aaaagcggca
     4141 atgtccactg aagctttttt actccttcaa aacgtttcca gcactataga cgatgcagcc
     4201 ttgcatgtca atccgcttcc ctgtttttca cgatacgagt catttcaggg ttgaaataaa
     4261 tcgaatcttg cgtaaactga tcccgacaac gctttaagga gattcccatg gcccgacacg
     4321 ttgctccacg catttcgccg tttcagcctt tcccggcatt ctttgcgcct cttccccgcc
     4381 gtcggcggac gggaatcttg tgaagccggt atccgcaaac ggggccgccg catcgaaaag
     4441 gcgggcgatt cggccgtccg ccgcgcgttg ggcctcggag cgcccaaagg gaaggcaggc
     4501 gtcctttaca aagcgggtat ccgccgtgtt tctgatggcg ctgagtctgg ccgcgatgcc
     4561 cttcgaatcc cggtcgtccg gtccggacgt gtctttttct tccgacgcca tggttcgcgg
     4621 cggcctgatc ctgctcaggg ttcagggctg cgaatccgac gatgttcagg tggtctggat
     4681 gggaaaggaa atcccccttg tccgcgaccg ggagggaacc ttgcgacatg gatttctggc
     4741 cgccgatttg aaccgggagc cgggagtgta tccggtggct gtgcgcgatc cttcttcggg
     4801 gtttgaaaaa tattttgaac ttcgggtcaa cgaccgggat tacggcgttc gcaaaatccg
     4861 gttgccccgc gccaaagtcg aaccggatga agcgtcgctg cgaagagtga aggcggaagc
     4921 gctggccgtc aacgccctgt ggaaggccga atgccggccc ccggaatggg aaggttcctt
     4981 ttggatgccg gtgaacaatg aaatcgtcgg cgccttcgga cagagaagca tcatcaataa
     5041 aaagaagcgc tccccgcaca cgggcgtgga catccggggc aaaaccggcg ctccggtccg
     5101 ggtgtccaat tccggcaccg tcgttctggc cgccgatcac tttttcacgg gcaagtccgt
     5161 ttacgtggac cacggaagct gcatcttcag catgtatttc catctggatc agattcgggt
     5221 tgaagagggg gaacaaatcg aaaagggagg gatcctcgga agcgtcggcg ccaccgggcg
     5281 agcagccggt ccgcatctgc actggggcgt gcagatcaac ggggccaggg taaatccttt
     5341 gatgctgatc gaattgagcc gggagttgga cccatgaacc gacggttccg gaagaaagca
     5401 ccccaaagcg ttccgcggcg tttgggagac ggtatccgtc atgacggccg gcttttcatg
     5461 ccggaaacac gaaaaatcgc ctcaaaaggt tgacatcgtt ttttgattgt atataatcac
     5521 aaccgttcgg gaggtacgca tgcgcgattt tcgaaatatt ttatacgcca tggatctgga
     5581 tgcggagcat ttttcttccc tggtgcgcgc gctggaattt gcgcggcgtt ttcaaagccg
     5641 gattcatatc ctctatgtca acgatccgca ggccggctat cgccatccgg cggaccatga
     5701 agatgccgtg gccctgaaag tcaaggaagc cgcgcccgaa tccctgctgg aaaatctgaa
     5761 ggtcgtttac gccgtctcca aaggcgacac ggccggagaa attgtccgat acgccggaga
     5821 aaatcagatc gacctgattg ttgtcggaca taaacaccgc ggcaaactgt atgcatcgat
     5881 gtttgacagc acggatgtcc acgtcattga ccaggccatg ctgcccgttt tagtttttcc
     5941 ggaaacgtaa attttcttgc ggggaagact cttaaggagc agcggccgtg cgcacacagg
     6001 aggattttgt ccatgaaaat caaaaaagac gcgaaacaga aaaggatcag gatcggtctt
     6061 ttcggtttcg gtaaaaccgg ccgcctggtg gcggaagagt tttttcagga cggcctgttc
     6121 gatctggagt gggtggtccg gaaaacccac aaggaaaacc acaaatatgc tacccgcctg
     6181 ctggggcatg aagtggatga tgcaagtgtt ttttcggtgg atgaagtgac cccggagttt
     6241 taccgccggc acccggtgga catcatcgtt gatttttcaa gcgccgccgc ccacaggcat
     6301 tacgctcccg ccgctgactt tggcatcaaa atcatttccg ccatttcgaa gtacagaacg
     6361 gaagacgtgg aggatttaag gcggatggct gaaaaaacgg cggtgctgta ttcgccgaac
     6421 atcactttgg gcatcaattt tctgcttgtg gcgtcgcaga tccttcaggg gatcgctccg
     6481 cacgcggata ttgaaattgt ggaagagcac tttcgcggca agaaggggga atccggcacc
     6541 gctttgaaaa tcgcggagat tctgggcctc gaaaaggaaa agcgcgtcaa ctccattcgt
     6601 gtgggaggga ttgtgggcaa acacgaaatc attttcggtc tgcccaatca gaccatccgt
     6661 ctggtgcatg agagcatcag ccgggcggcg ttcggccagg gcgccatttt tgccgccaaa
     6721 tggctgatcc acgctccgcc ggggttgtat tccatggaaa acatcatcgt ggacatgttt
     6781 aagaaaaaca ttcctgttta ctagagttcc atcatcatca gcggatcgcc gatttccacg
     6841 gacgcgccgg gggaggcaaa gatttccgaa accttgccgt ccacatccgc cgtgatattg
     6901 gtgagcattt tcatggcttc caggacgacc agcgtgtcgc cgacggcgac ctcgtctccc
     6961 accgccacgg gaatctggtt gacgacgccc ttcagggttg cgcggatatc gcccattctg
     7021 gccagcgccc cgatttcctt ggcggaaagg accacggagg ccttgggctt ttctccctcg
     7081 tcgtcggccg gcgtgacgac gggttcgggg tggtaatcca cgcccagatg cgtcatatac
     7141 gagccgtcct tggtgcggcg ggaagggccg atttcaatgg tgtgcagttt tccggaggcg
     7201 tcggggaatt cgaatttgtc gccgatgccg aatccgccgc gccggaacca cactttgggc
     7261 ggcagcaccc atgtcttgcc gaatttttcc tcaaacttga agaagcccac cgcgtcgttg
     7321 ggatgctgca gatacagcac cagttcgtcg tcggtggcct tccggccgat cgcgtgctcc
     7381 agaatccgcc tttcgacgtc cagatcaacg tcttcaattt tctggtagcc ggcttccctt
     7441 ctgacaattt ttttccagtc ctttccgaaa accgcctcat aaatctccgg cgccggcttg
     7501 taaaacggaa acttgccgta gcgctccagc atcagatttt tcaaatcatc gctggcgtcc
     7561 cggtagcgcg ctcccttcac gacattgttg gcggccgtgg tccacaaaat ctgcgaaccg
     7621 ggggtgacgc tccagaagtt gcccagttct acctgtacgc ggggcagttc gttttgcagg
     7681 atttccggca tgcggtccag aaaaccgcct ttttgcgcct gttcgaagct ggaccccgtc
     7741 gcgccgcccg gcagcttgtg ggtctgcacc gtcgggtcaa atccgaagaa ctgcgattcg
     7801 aaatcccggt agtaggggcg ttcgtgccgc agtttgttgg aggcttcgat gacggcctgt
     7861 ttgtccagtc cgacggcgtt gtagccgtaa tcctccagcg tgttcaccac cgtcaacaga
     7921 ggcggcgggc cgtagaagcc ggtcagcgcg tgatcggagg cgtccacgat ttttgcgccg
     7981 ttccgcacgg cggccacgat gcggccgatt tcattgccgt cggtgttgtg tccgtggtag
     8041 tgaatcagca gatcgggata cttctcccgg atggcgcgaa ccagttcgcc gatgcgtccg
     8101 ggcgacccca gtccgccgtg attcttgatc gccagcagaa tatccggtcc ggtaacgtcc
     8161 agaatttcgc ccactttcct gatgtaaaac cggttggtgt gctccggccc cgtcgaaaag
     8221 cagacggccg cctcgagaag gcgtcccgct ttccggactt cttcgtaaac ggggaccatg
     8281 ttggggatgt agtttaaaaa gtcgaacacc cgccagacgt ggacgcgacg ggcaaaggtc
     8341 tccacggtga ggcggatgac gttttccgga tagggacggt agccgaacag gttgatgccg
     8401 cggcagagcg tctggaaaag cgtatccggc atatgcccgg caaggacttc gagtttttcc
     8461 agcgggttca tctggcggcg cagcatgtcg acatgaaccg acgcgccgcc cgtgatctcc
     8521 agcgaaaaat agccggcttc gttgcgctcc ggggcgacaa gcatctgcgt gtggggcaaa
     8581 atccggtttt taaaatccga ctgggagaga tcgcgctccg tgttggtgag ataatagccc
     8641 ggcatgcggc gcattttctc caggacggct ttggccccgg cgctttgtag atcgcgggga
     8701 gtgattcttc cttcggggag gattttcatg caatacgtct ccttgcagct ttgcccggtc
     8761 agtaggcgta ggggttgatt ttccgcgcgt ggatttccgc gatcagtttg gagagtttgg
     8821 cgatttcccg ctccggctcc gggtaatcga acagttcggg atgggcctcg aggtaatccg
     8881 tcgtaaagcg gccgtcccgg aaatccttat cgtcgcagat ggccaggtaa aaggggatcg
     8941 tcgttttcgg tccgacgatc agaaagtcct tgagggcgcg cttgagccgc tggatggtct
     9001 gctgccagga atagccccgg acggtcagct tgaccagcag cgaatcgtag tcggtgggaa
     9061 ttttgtagcc ctggtacacg atgccgtcga gccggatttc cgggccgccg ggcgattgat
     9121 aaacctcgac gcgcttgccg ccttccggca tgaagttgtt tttggggtct tccgcgttga
     9181 cgcggacctg aatggctttg ccgaccagat ggatgcgctc ctcgggaatg tccagcgtct
     9241 ccccctcggc catcagaatc tgctggcgga taatatcgat gcccgtgagg atttccgtta
     9301 cggtgtgctc cacctgcagg cgggtgttga cctccataaa ccagaaccgg ttggtccggg
     9361 gatccaccag aaattccacc gtgccggcgt tgacatatcc cgcttcacgg gccgcccgaa
     9421 tggccgtttg gtgcatggcg tcgagcacgt ttttcggcag gtcggccggc gcgatctcca
     9481 gcagcttctg gtggcggcgc tgaatggagc agtcccgcgt gcccagatgc atcacatggc
     9541 cgtaggcatc ggccagaatc tgcacttcga cgtggcgggg cgactccacg catttttcaa
     9601 tgtagatgcg gtcgtcgttg aaggcggaac gcgcctcgga gcgcgcctgc ggaagctgcg
     9661 agcgcagctc ttcctcgctg tcgattttgc ggatgccccg tcccccgccg ccggccagcg
     9721 ccttgagcat gatcggatag ccgtgcgtct tgccgaaggc cacggcctcc gtgaggcccg
     9781 cgtcgcctgc ggaaagcgtg ccggttcccg gaacaaaggg aatggctgcc ttttccatga
     9841 tgttgcgcgc gacgaccttg ttgcccagat cgcggatcac ctggggggga ggtccgataa
     9901 acgaaattcc cgcatcgacg caggcctggg ccagatcggg attttccgac aaaaaaccgt
     9961 agcccggatg aatggcgtcc gagccggatt tctgcgcggc ccagatgatt ttatcgatat
    10021 tgaggtaatc cctgcgcggc ccgtctccca tcagaatggc ctgatcggca aatcgcagaa
    10081 aataggcttc tctgtcgggt ttttcataga cagccacggt atcgaggtcc atttccttga
    10141 ccgtccggat gatgcgcagg gcgatttccc cccggttggc caccagtact tttcggattt
    10201 ttttcataaa cgcccttcct tgccgtcgtc gtgatcccgt cctgacgatg ggaaggttat
    10261 acttttctca cgcaacggct gtcaagcaca tagtctgcaa tcaggatttg cttattttga
    10321 cgaaatattt taacgtggcg cgggctgatg gagggcatcc ctatgaaaga ctgcaccatg
    10381 gcgggtttct tcgattatcg cagcaaagtg atcggcacgg atcaggtgct ggaaaagctg
    10441 aagcccgggc agaagatctt tttatccagc ggtgtcgcgg cgcccgggaa agtcctgacg
    10501 gcgctggccc ggtcggacgc gaaaaatctg cgcgacctgg agatcatcca gctcattacg
    10561 ctgcgcaaat acctgtctca ccagagctcc gaccagtacc ggctgaaaac gttcaacgtc
    10621 ggagaaagca tcagcaagga agtcgccgag ggccgggtcg atttcatccc cgccaacctg
    10681 atggagctgc cgtacatttt tttaagcggc gcggtggcgg tggacgtcgc gatcattcag
    10741 acgtccgtgc cggacgacaa gggcttcatg tccctgggtg ttgcggtgga cgtggccaat
    10801 atcgtcatca agcaggcggc gctggtgatc gccgaaatca atccaaacat gccggtgacg
    10861 ctgggcgaaa cgaccatcca catcagcagc atcgactatg tcgtcgaaag cgatctgccg
    10921 ctggtcgagc ggatcaaccg gccgtatgac gagattacgg accgcatcgg ctggaatatc
    10981 tccaacctga tcgacgacgg ggcgaccgtc gtgctgcacg tgggcagact gttcaacgcc
    11041 gtggccgatc acctgaagca aaagcgcaac ctcggcatcg taacgcatgt ggtttccgac
    11101 tgggcggtgg acctgattga atccggcgcc atttcgctgg accgcagccg gttcaacggc
    11161 ggactgatta ccgccagcta ttgctacggc agcaaggcgc tctaccggta cgttcaccgc
    11221 aaccccatct ttgaattcta tccgatcgcg aggctcaaca atcctttcgt ggtgcgccgg
    11281 atcaaaagcc tcatcggcat catgaacgtg aaaaagatcg acatctcggg cgagtcggtc
    11341 atcttccatt ccggcgacaa tctgctgacc ggctacgaaa gcaagctgaa cttcgccgtc
    11401 ggcgcgacca tgacccgaag aggcaaggcg atcgtggcgc tcaattccgt ggatacggag
    11461 ggaaacagca acatcgtcat caagtttgac ggccggaccg acaacaccca cagcacactc
    11521 ggcgtcgtga aatacatcgt caccgaatac ggcgtggcca atcttttcgg gaagtccatt
    11581 cgggaaaggg cgctggccat gatcgacatc gcccaccccg accatcgcga agacctgctc
    11641 aggcaggcca aagcgctcaa ttatgtttat gcggaccaga tttatgtcgc ccgccacgcg
    11701 gccaactatc cggcgctgtt ggaaacgcgc aaatccctga aggactctct ggaaatcaag
    11761 gttcgtccga tcaagccctc cgatgaggac aagatgcggc ggctcttcta caacttttcc
    11821 gacgaatcga aatatttccg ttattttgct ccgaagccgg tcatgcccca caaggaaatg
    11881 cagacgtatg tgagcatcga ttatgacaag gttttgtccg tcgtggccgt cgcggaaaaa
    11941 gggcggaatg agaggatcat cgcggaagcc cgttatgcct atgacaaaag cgccggcgcg
    12001 tatgagattg cgttcattgt cgatgaggac tttcagggaa ggggcgtggc cacctttttg
    12061 tttaagtatc tgatcaaaat cgcgcgggac cgaggaattc aatggtttat cgcctatgtg
    12121 ctgccgcgca atgaatccat gatgaaagtc tttgaaaaat caaaattgcc catacagaaa
    12181 tcgtatgaaa acgatgcgct ggttttgcgt tttgatctga cgcaggaggt tgcggatacg
    12241 gatgcggccg caagaaagta agcgcgccgc ctcgcaattc attcttgcca taacacccgg
    12301 attgtggaat acttcgccgc gaatccggcc gattcgtaaa gcattacgcg cttttggttt
    12361 tgccgggttg taaaaataac atcaacgata tcaatcctat cgagggaggt ggcagaatca
    12421 tgtcaaatgc agtcattgtc agcggtgtgc gcacagcggt tggggccttc ggcggttccc
    12481 tgaaagacgt tcccgccaaa gatctgggag cgctggtgat tcgtgaatcg ttaatcaaag
    12541 ccggtttcaa gccggttttg ccggcccatg ccaaagaaga cgcgccggat acggccaaaa
    12601 acgagggcct ttgcccggtc gagcagcagt attccaggtg ggacgccaat ctccgggaaa
    12661 ttgccgtgga cgaagtgatc atgggcaacg tcatcggcgc gggccagggg cagaacgtcg
    12721 gacgccaggc gatgatccgc gccggccttc ccaaggaaac gaccgccatc accgtcaaca
    12781 aggtatgcgc ctccggcatg aaagccattg cgctcgccgc cgccaccatc aaggccggcg
    12841 acaacgaagt ggtgattgca ggcggcatgg aaaatatgag cgacgtgccc atcggcctgc
    12901 ccaaggcccg ctggggccac cgtatggaca tgccgttcgg caaaaccgtc gatctgatgg
    12961 tgtttgacgc cctctatgaa atcttctacg gctatcacat gggcgttacc gcggaaaaca
    13021 tcgccgcccg ctacaacatt tcgcgcgagg agcaggacaa actcagcctg gagagccata
    13081 cccgtgcgcg caatgcgatc aaaagcggcc ttttgaaaga tgaaattgtt cccgtgacga
    13141 ttccccagaa gaagggcaac cccatcgtgt tcgacacgga tgagcgtccc atggaaacca
    13201 gcatggagaa gctggccaag ctgggcacgg ccttcaagaa agacggcacg gtaacggccg
    13261 gcaacgcctc cggcatcaac gacgcggcgg ttgcgatgct gatcatgagc gaggacaaag
    13321 ccaaagcgct gggggtgaaa cccctggccc gcatcaaggg ctacgcctcg ggcggcgtcg
    13381 atccggctta catggggctg gggcccgttc ccgccgtccg caaactgctg cgcatcaccg
    13441 gcacgtcggt caaggattac ggcctgtttg aactcaacga agcgtttgcc tcccaggcca
    13501 tttcctgtct gcgcgagctc aatattcctt gggacatcac caacgtcaac ggctcggcca
    13561 tttccatcgg tcatccggtc ggctgctcgg gagcgcgcat cgtgctggcg ctggccaatg
    13621 agatgaagcg ccgcaacgtg ggcctggggc tcgcgtctct gtgcatcggc ggcggacagg
    13681 gcatggcgat tgccatcgaa gcagtttaac aaatatttta tatcgtaggg aacggcttca
    13741 aaccgttccc tatattttta tggagtggtt catgccttat caaaccgtca acggcatcaa
    13801 aatctattac gaagaaaagg gcacgggtga gccgctgctg ctcatcaacg gcctggcttt
    13861 cccgatggac ttgtggtttg cgcagatcga ggagttgtcc aaagacttcc gggtgattgc
    13921 gctggacaac cggggcatcg gccggagcga caagccggac gatgtgtatt ccatgtcgtt
    13981 gatggcctct gatgccgtgg gcctgctccg gtcgctgggc attcaaaagg ccaacgtggc
    14041 cggcctgtcc atgggcggct ttatttccca ggaaatcgcg ctttcctgtc cggaaatggt
    14101 caaccggctg attctggtcg cgaccggcat gggcggcccc cggtcgctgg cgctgggaaa
    14161 gcccttctgg gatagagtcg cagccgccat tgccggaaag aatccctggg agatctatcg
    14221 catcgatctg acgctgatga ccgcgccggg atttgtcgag gagcatccgg agaccctgga
    14281 taaagccgtc gaactgcgca tgaaaaaccc gcagcctttc catgcctttg tacggcagtt
    14341 tacggccgcc ggtttgtttg acagcaacga acgggtgcaa aaaatcgagc atcccacgct
    14401 gatcgtttta ggacgggagg atccgatttt tccgattgct ctggcggagg atttccggga
    14461 aaagatgcct catgcgaaga tgatcatcta cgaacaatgc ggccacgcga tccttttgga
    14521 aaaaccggac cggctttcaa aagatataag agaattttta aaagggaatt agaccgaaga
    14581 cggaaggccg aaggtctatt ttgcaggcct tcggccttca ttggaacggt gccgacagcg
    14641 cctgcctttt tagtcttcag ccttcggtct tcagtctatt tgcgatatgg gaatcaaaaa
    14701 catcaaaacg gtttcgcctg accaagccgt ccgggccgtt cgctccggcg atcacgtgca
    14761 catcagcgcg acctccaaca ctcccgcgtg cctggttgcg gcgctgtgcc ggcgcggccg
    14821 ttcgggcgaa cttcgcggag tccatattca tcatctgcac acggaaggcc ccgcgcctta
    14881 cgccgatccg tcgtttgaag gcgtctttca gcttgagtcc ttttttgtcg gccccaatgt
    14941 ccggctttcg acccagcagg gatacgccga ctacattccc gtgtttctgg gagagaccca
    15001 gcgtctctac cgtgaaggat acctcccgtg cgacgtggcc ctgattcagg tgagcccgcc
    15061 cgacgaacag gggcgcgtct ccttggggac gtcggtggac gcgtccgtgg ccgccgtcga
    15121 aaaagccggg gtcgtgatcg ccgtgatcaa tccgcacgtg ccccacacct acggagacgg
    15181 cctcattcca accgatcgca tccacgtttt cgtggaagac gacacgccgc tggaaacggt
    15241 tcatttcgcc ccgcccggtg acgtcgaggc ggccatcggc gaacactgcg cttcgctcat
    15301 cgaagacgga gcaacgctgc agatgggcat cggcgcgatt cccaacgcgg tgcttgcgca
    15361 gctttccggg cataaaaatc tgggaatcca cacggaaatg ttcgccgacg gcgtgattga
    15421 tctggtcgaa aagggcgtca tcaacggggc gaacaaggcc atcgaccggg gccaaatggt
    15481 ggcgacgttt cttctggggt ctaaaagatt atacgactac acgcaccgca acgaccgggt
    15541 gttgatgaaa gatgtcggct acaccaacga tccgtttgtc atcgcgaaaa acccgaaggc
    15601 cacggccatc aactccgcgc tgcaggtcga tctgaccggg cagatctgcg ccgattccct
    15661 ggggacgaaa tgctactccg gcgtcggagg gcagatcgat tttttttacg gcgcgtcgcg
    15721 ctcggcgggc ggaaaaccca ttttagccat gccgtcggtg acggaaaaag gggagagcaa
    15781 aatcgccccc atcctgcttc ccggcgcggg cgtggtgacc acgcgcgccc acgcgcactg
    15841 gctggtgacg gaattcggag cggtgaatct ttacggccgt tccctgcagg agcgggcgcg
    15901 gctgatcatc ggcattgccc acccggacca ccgggaagcg ttggatcggg ccgcgttcga
    15961 acgcttcggt ccgcactacc attttgcgcg catatgacgg accggatctg atccgcgcga
    16021 agtcttgttc aattttttgt tttctgctac actgcactca tgaataaata caatatccat
    16081 cgcaaactat ccccgcttgg cagttgttca aaaatctttt tcacccgtca acgcaagcct
    16141 gcacacgaaa gaatgatcgc atgaaggatc acgccaagac caaacagccc atgatcggcg
    16201 aactggcttc cctgcgcgaa cggattgcgg agctggagcg ctccgcgctg gagcaaaagc
    16261 gggtggagga ggaactgagg gaaagcgcgg agaaataccg gctgtttttc gagtactccc
    16321 ccctgggcaa tttttacttt gacgaaaagg gcgtcatcgt cgcgtgcaac gaacacttcg
    16381 tcgaccttat cgggtcttcc agaaaagcgc tgatcggcct ggaattattc aaactgccgg
    16441 atcaaaaggt tgttgaagcg gttcggcagg cgctgggcgg ccaaaccgcc ctttatgagg
    16501 atgtgtatcg ttccgtcacc gcggacaaag ccacgccggt aagggctgtt tttacgccga
    16561 tccatctgga gggcaggggc gttatcggtg gtgtggggat tgttgaagat atcaccgaac
    16621 gcaggcggtc ggaggcggcg ctgcgggaga aagaagaacg ctatcggtcg ctggtcgaaa
    16681 atgccgatga ggcgattctc gtgatccagg acggcaaaat tcaatttgtc aacacccggg
    16741 cggtcgcggc ttttggatat tcggtgcagg aatttatctc catccctgtc tttgagctgg
    16801 tgcatccgga ggacagggaa atggtgatcc ggcggtatct tgagaaaatc agcggcgatg
    16861 ccgcccccac ccggcatacc taccgggtgc tccccaaaaa aggcctcgtt tcgtggatag
    16921 agatcagctc catcctcatc cattgggaag gcaggcccgc gacgttgaat ctcatcacgg
    16981 atatcaccga tcgcaaaaag gcggaggacg ccctgcggga aagagaagag aagtaccgga
    17041 ccattctgga aagcatggaa gacggctact tcgaacagga cctcgccggc aactttattt
    17101 tcgtaaacga ctccatgtct cgaatctacg ggtatccgaa agaagaatta atcgggatga
    17161 attacaagaa gcataccgat gcggaaagcg gcagaaaatg ctttcatatc tacttgaata
    17221 tttacaaaac ggggaagccc ggcaaagtcg ttgattttga tattatccgg aaggacgggg
    17281 ccagaaggca tgttgaatca tccgtgtctc ttatccggga caacgcggga accccgatcg
    17341 cctttcgggg cattgtgcgg gacgtcacgg aacgcaagca ggcggaggag gccttgaatc
    17401 agagctttgc caggctgcgc gccgcgctta ccgccaccgt tggggcaacc gccatgatgg
    17461 tggaaaccag ggatccttac acggcgggcc atcagcgccg ggtggccgac ctgggccggg
    17521 ccattgccga agaaatgcag ctgaccgacg agcagatcga aggcgttcat atggcgggca
    17581 tggtccatga tctgggcaaa atctccgttc cctccgagat cctcagcaag cccaccaggc
    17641 tcaccgatct ggagtgccgg ctcatcaaaa cccacccgca ggccggctat gagattctca
    17701 aagacatcgc gtttccctgg cccatcgcca gaatcgttct ggagcaccat gagcggatgg
    17761 acggttcggg atatccgcat ggcttaaagg gggatcaggc gcttctggaa tcccggatcc
    17821 tttccgttgc ggacgtcgtg gaagcgatgg cttcgcatcg tccctatcgt gccgggcttg
    17881 gcatcgaagc cgccctggcc gagatcgaag cgaatcgggg gattctctac gacctgtttg
    17941 tggtggatgc ctgtctgaaa ttgtttcgcg aaaaaggcta tcgctttccc gaatcctgaa
    18001 aaccaatgtt ttccaaccgc attcgctgac gcaaaccctc cggctgtccg gaatggaaac
    18061 gcttatcaaa acctccctca cccgctacca gcttgtcgaa ccctggacag cgtcctgata
    18121 atcggtctgc ccgtgccggt ggcggggcgg aaggatttgc acggagtatt tgcagaccgg
    18181 gtcgttgtcc ctgcgccaga tattgacccc gtattccacg ggaacggaaa catagccgag
    18241 tgtttcatct ccgtcccgaa tggcgtaatt ctgaaagtgc gctaacgcct gtttctccgg
    18301 tttttccaga tcatcgaggc tttggatgac ccgctgacgc ggaaaatccg cgcatatggt
    18361 ctctttcccc gtgacatgga caaggccggc aagcgttttg tcgggcgccc cttcgggatt
    18421 ttccaacttg atgatgcgtt cgtatgaagc gtcgggaatg ccggagactt cctgcttttg
    18481 aaggattccg tagccgctgc agcccaccat cgtcatcaaa agacatgccg aaacgctttt
    18541 gatgaaattt ccaatgcgat cactcatctt tcccgtccgc ctttcccgga agcgggcatc
    18601 caagccggtc tggccggccc cctttccggt gggatcaggc tactaccgtc cggaaggctt
    18661 gtcaaggacg gccgaagacg gccgacgata cggccgggca atttcctgaa atatacattt
    18721 tttccttcgg cttttttgtg gtatagtgcc gccgctttgc gacagaaaac gcctgcgcga
    18781 tacggaacgg aaaacatgaa agtattcaaa atcaaatttg ggaagagcgg cgaaaaagca
    18841 gatgcgttcg gaatacgttt tttgggggac tttgacggca aaatggggca aaatggaagc
    18901 ttcagcgaaa tcatctcccc gccggactcg gcgcccctga taacgcatca aaggattacc
    18961 atctggttta cgtttgaaag aatcagaccg tacgacaccg tctccggctt cgtccgcgag
    19021 ttgtcggaac tcctgaaaga gaaggattac atcataaaat attcatttat tgacgggctg
    19081 ttcaacacga ccctgctgga tgacgcgaat ccaccggaag ggttttcgga cccaagagat
    19141 cccggcaatg ccgcgaccgg tttttccgtc acggccgaaa aacacagcgc caagctgaaa
    19201 tttacagtga atgagatcgt gtcggtcagt gaagcggccg cgaaactggg ccgcacgatt
    19261 tacggcagaa atctggagag agtcagagtc aaccggtaac cccggtccaa catcgacaac
    19321 agaaataaaa atcaccggat cacccgtttt ttcaactgct cggtcaggcg ccttagcctg
    19381 ggcgaaatat cgtatttatc gagtttgaca tcaatgacgg ccagttcatc gccgttgttt
    19441 ttcgccgcgt gcagcgcccg ccagagctcc tcttcgttgg tcgcagtgta tcctttgccg
    19501 aagccgatga cgcgcggcaa atccgcatat tgccaggggc acaaatcgtt aaacggaccg
    19561 tcgatcatgg agcggaaggt gccgtatccg ccgttgttca aaacaagcac tatcggagcc
    19621 aggccgcagc gggcggcggt ggatatttcc atgccggtca tctgaaacga tccgtctccc
    19681 accaaggcga tgacgcgacg atccggcgcg gcaagcgata cgccgacggc gcccggcacg
    19741 gcaaagccaa gcaaagcgta ataggccgga gacatgaagt cgcttgctcc ctgaatgaaa
    19801 agatccagac cgccgaaaag cggatctccc ggttcggcaa tcacactcgt atgatcgtcg
    19861 agaagcgagt tgacgcaagc gatgaggctc tccatggtca ccttgcagcc ggtctgcggc
    19921 acaaaaggag cgggcgcgtt tttgggcgtc cactttgttt tgggaggtgt tgcggaaagt
    19981 ttggcgcaca aagccttcag cacgtcggcc attcccaaac cgggatagat gtgatgccgg
    20041 acgcaaaggg aatcgggaca ggcgacaacg gctttgcccg gattgagcgt gcccgtggtg
    20101 aacatgccgg tggacaaatc cgtgatcaag agtcccgccg ccagaatgaa atccgcgttt
    20161 tcaaccatat tgcggacatc gtcggggctc atcgcgcccg cgtaaacgcc gacaaactgt
    20221 ggatgcgact ccgcgatcac ccctttgccc aggatgccgg tgacgaaagg cagtcctgat
    20281 cgctcaagaa agtcgaggaa aagtcgctgc agaccaaaac ggtgaatatc gatgccggca
    20341 acggcaatcg gccgttttgc ggcgcgaagc atcgaaagca cttcctttaa ggcttcgttg
    20401 aggactttgg aggtgtcctg cttgtctttc ggggtgtttt tcggtggaac gtcggtttcc
    20461 ctgctgacca tgtcgcgggg cagctcgaga tatccgggcc ttttggtggt cataacggca
    20521 tcgatcacgc ggtcgatttc ggcggcggcg ctctgggcgt ccgtcagcgc ggcggttgcc
    20581 gccgtcattt cccgaaacac gtttcgctgc gtttcgaagg agcggacttt gtggtgcaga
    20641 agcgcgtcgc cggatcgttc cgacattccg ggagcgccgc tgatgaccag cacgggagag
    20701 cgctccgcaa aagcctgggc ggtgctgttg gcgagtttga ggccacccac gccgtaggtg
    20761 acgcacacga ccccgaagcc cgacattctc gcgtaagcgt cagcggcaaa tcccgcacct
    20821 tgctcgtcgc aggtgttgat gagcgtcaag tcgcttttat ggagttggtc gtagaaatgg
    20881 agcacatagt cgccctgaat gccgaagacg tgacgggcgc cggcctcttg aatcgccttg
    20941 atgagataat tgccgatgag catggttcga cctcctgaga ttcaaaaacg gggtgatccg
    21001 gcctgtgtga acggttgaac gggtccggtt ttgccgtcgt gcagtttctt ttccgctccc
    21061 cttcatggtt cgttgcattt cttcgagggt aatatcgaag aacgatttgc gacaaccgtc
    21121 ccggcattgt ttgccaaaac gtcgacggct ccgaaattcc ggcgaatcct gtcggcggtt
    21181 tgcgccactg cctcgctctc ggcgatattg cacaaataat acgacgcgcg cacattacgg
    21241 ctcgatattt cccgggtgac gctttttgcc gcctgttcat tgaggatttg aacaactttt
    21301 tcgcgggatt gcgcaacggc ctccgtccac caaaggggca aaagcagaaa agaggtaatg
    21361 actgccgcgg ggaccttccg tttcgtcatg actcctgacg gcgcgcttac gcgcactcgc
    21421 tgtagccgca gaggcgacag acggcgcagc ctccctcatg ttcgatgatg ccgccgcagt
    21481 ccggacaggc gcctttgagg aaaacgcgct tcacatgctg caccttgccg ccgtttgccg
    21541 ccacatgcgc ctgaatcgct ttggccaccg cgtcggcgca ggacaaaacc ttgttttcac
    21601 cgaagccgga cggagaatgg caggaaatgc cctgcaactg tttgacgacc tgacgcgccc
    21661 caagtccgct gcgccaggcc agcgacacca tgcgtccgat cgcttcgctc tgggaggcgg
    21721 cgcatccgcc cgctttgccc atcgtcgtaa agagttcgaa aagacccgtg tcgtcctcat
    21781 tcaccgtgac atacagcggt ccgcatcccg tctgcatctg ataggtccag cctttgagga
    21841 ctttgggccg ttcgcgtttg gtggccggct ttccctgggc ggcgcccgct tccgcctctt
    21901 cttttttccc gacgctcaga acctgatggt cccttgagcc gtcccggtaa atggtgacgc
    21961 ccttgcagtc gagcttgtag gccagttcat agacgctggc cacatcctgg atcgacgcgc
    22021 ttttggcgaa gttgacggtt ttgctgaccg cgttgtccgt gtatttttga aaggcggcct
    22081 gcatccgaat gtgcacttcc ggcgtaatgt cgtgcgcggt gacaaacacg tttcgaattt
    22141 cttccggaat ttccgtgatg ccgtgaagcg tgccgtgttc ggcgatccgc tgcatcagct
    22201 ccggtgagta aaatcccttt tctttggcca tggcttcaaa gaggggatgg acttccagca
    22261 gaatatcatt gtccattacc tgacggacgt aggatacggc aaaaaggggt tccacaccgg
    22321 aagaggcatt ggcgatgatg gaaatcgtac cggtgggtgc aatggtggtc acggtggcgt
    22381 tgcgcagggg cggctcgcct tttttgtcgt aaagggaccc tttgaaattc ggaaaggcgc
    22441 cccggctccc ggccagcgca cgggacgcgg catgcccctg ttcattgata aaccccatga
    22501 ttttgtgggc cagttcaatg gcttcttcgg tattgtaggg aatgcccagt gcgatcagca
    22561 tatccgccca gcccatgacg cccaggccga ttttgcggtt gcccatggtc atttcgtgga
    22621 ttttcggcag gggatagttg ttgacttcca cgacgttgtc caggaaatgg acggccagtt
    22681 gaaccacgcg gcccagtctt ttccagttga tcttgccgtt ctggaccatt ttgcccaggt
    22741 tgatggagcc caggttgcac gattcatacg gaagcaaagg ctgctctccg caggggttcg
    22801 tggattcgat ttgcccgatg tggggtgtcg gattgtcccg gttgagccgg tcgagaaaaa
    22861 cgatgcccgg ctccccgttt tcccaggcct gcgtgacgat gcggttgaaa accttgcggg
    22921 cgttgagcgt cccggaaacc tgattgtcgc ggggattgat cagatcgtag ttttcgtctt
    22981 tttccaccgc tttcatgaaa gcttccgtca gccccacgga aatgttgaaa ttgttgagct
    23041 gctttttgtc ggccttgcac atgatgaaat ccatgatgtc cgggtgatcg acgcgcagaa
    23101 tgcccatgtt ggcgccccgg cgcgttccgc cctgcttgat cgtttcggtc gctacgtcga
    23161 aaacacgcat gaaggagatc ggcccgctgg aaatgccggt ggtggtcaga acgacatcat
    23221 tggccggacg caggcgcgaa aaggaaaatc cggttcctcc gccggatttg tggatcagcg
    23281 cggtgaactt cacggcgtca aagatctctt ccatggaatc gcccaccggc aggacaaagc
    23341 aggcggacaa ctgtcccagt tcacgcccgg cattcatgag cgtcggggaa ttgggcagaa
    23401 attccagttg ggtcattatt cggtagaatt cttccgcgat cgcggtcgta tcctcttttt
    23461 cattaaactt cctgtccgcg gcggcgatgg tatccgccac gcgccggaac atttgaaccg
    23521 gagcctccag cacctttccc tctttgtcgc gcttcagata gcgccgctcc agaaccgtaa
    23581 tggcgttttt tgtcaattcc gggacgatcg gggcataggt cttcttttcc atgaaaatga
    23641 actcctttgt gtatgaaaat aagagggtta gtaaacaatt aaaaaatact acatattgta
    23701 tgatggcttc cattaaccac aacatctagg cattgtcaat agaaggaaaa gaattatttt
    23761 gaatttttca aaaaggcaaa aaaaggcgcg tggttggaag tgaaaaattc cggcgtgcgc
    23821 cggatgatgg gagcgacagc cggatgcttt tttaatgttt tttcaatgaa attgcgaaca
    23881 ttcccacgct gccatccggc ggggtgcaaa aagcttcggt acagggacag atcgccttca
    23941 taaaatttct gtgtggacag gctgcgggct tcttcgctcc cggccggcag attgaaaatc
    24001 gccagattca aaaaagaaat atattcggaa tgccgaacga tgaaatccag cgtttcgtgc
    24061 gcgtctgcct ccgtttccgg cggcgtgccg aacagaaaat aaccatagac ggcaatgcct
    24121 gcgcgatgga gcgcccggag cgcagcagag gcttcggcaa gatcgatgcc tttggagagg
    24181 gcatccagaa ctccctgatg accggattcc atgccgagct tcagcatcgc gcagccgctt
    24241 tttttcagcg cccggcaaaa atcctcatcc gccagatgcc tcgttgcgcg ggcaaagccg
    24301 taccagggcg aaccgggcgg attttcgatc agcgcctgca ggagcgcggg agaaatagag
    24361 ctgtccagaa gatgaatcag ggacgcctgc gtctttcgcg caagcgcttc caattccgcc
    24421 gcggcgcggg aggccgacag aggccggtag ggatttgctt ccgccttttc cgggcagaaa
    24481 gaacattttc gccaccagca gccctcggcc gcgctgtagg gcaggataaa accgggagaa
    24541 aaatatcggt ttttttccag caggtcataa tcggccgagg ggccttcccg gcgagggggc
    24601 atgcccagca gcgccaagag tttttcctcc cccgctcctt ccaccattcg gtcaaccagg
    24661 ccggaaaaca gattcgttcg ccccgaaaga cgaatccagg aagttgcgag cgatccgccc
    24721 aggagaattt tctgccgcgg atgaattttt ttgacgaagc cgatcatggc catggtgcag
    24781 agcgcctggc ttaagaaatt catggagaag ccgataaagt ccggagattc ctcaagcgcc
    24841 tgcgccagac gcgcggaaaa gtaagaataa aaaggattga cttccggctg ttcggccgcc
    24901 tgaattaaat caacgcttct cagcggggag cgtcggccgt cttcatagtc ggacagagac
    24961 aattgaacgc cgaagggctt cccggccgtg tgcagaacgc ggtgaatatc catgacggcg
    25021 cgggaatacc ggctttgatt gcagaaagta ccggggtttt gcagcgcggc caggttttcc
    25081 tccaggtttt tgcaggcgcg gcgactccag cggccgacag gcgtttccgt gcgggcgaag
    25141 gcgcgcatca gatggagcat gccttccaga ttagcgtcga tgacgccgtg ctcgacgctg
    25201 ttggctttca ggcaggcgga aagtctggca atgcccgccg gcggttcgga gggtttcacg
    25261 acgggaggat gaatgaaaat gatctttgca gttgtcatcg gcgaatgttt agtattgaat
    25321 gacgccttgc gcaagcaata tctcatgcct atatttgagt tgacagggcc cgggctttac
    25381 gcaatagttc cgaccgtgaa gaaaaaacaa ccattgaaga gccttttgtg aaaattaacg
    25441 aaccatacga tttgccggcg cggctccgaa ccttacttgt tctgtgtgta tttgtcctga
    25501 ttgcgctgac ctcttttgct gcggaagcgg cggatcggtt ccctgcgcat aagggaacgg
    25561 ccgtcaatga ttttgccggc gtgatcgatc ctgaaaatgc cgcaaaaatg gaatccctgt
    25621 cgcgggaaat tctggagaag acaggaacgg cgatcgtcgt ggtttcgctg gcggaactgg
    25681 ggcctgccga agaaatcaac ctttatgcga gcggtcttta tcaggcctgg gggatcggga
    25741 aaaaaggcga ggacaagggt gttttgattc tgctggccgt caaggagcgg aaaatccgca
    25801 tagaaaccgg ctacggcgtc gaaggcgtcc tgccggacgg cagggtgggc gcgatcctcg
    25861 atcaggtcgt ggcgcctcat ctcaaggcgg gggagaccgg caaggcgctt tacaatgcaa
    25921 tgctggcctg cggcgcctat ctcgccaaag acgcgaaggt tcagttgacc ggcgatttct
    25981 ccttttaccg cagcgccgcc cgcccggaaa agaagggcat caatattttg ggaatcctgt
    26041 tttttctgat tgccgcggcc gttcttctgg gaacgagaac aggacgggag atgctgccct
    26101 ggattctgtt gatggtgctt tccggcggcg gccggggagg cggctccgga ggcttcggcg
    26161 gcgggtttgg cggtttcggc ggcggcatga gcggcggcgg cggcgcaggc cgcggttttt
    26221 aaaacgaagg atggagaaca tggcaagaat accgaatcag cctcaagaca tttttgttcc
    26281 cctgaccgag gactggctca atgccttcgg cagggatctg gtttctctga tgctttacgg
    26341 cagcgcggcg ggcggctcct atgtgaaggg aaaatcggac atcaatcttc tggtcgtcct
    26401 gacgccggaa ggcatgagcc gcctggaaga cgcttttgcc ccggtgaaaa aatggagaaa
    26461 gcgcaatgtg gccgtgccgc tcgtcctgac gcaggaattc atttcttcct cgctcgacgc
    26521 ctatccgatt gaatttctca acatgcagaa ccattgcgtt ttgatttacg gggaaaatgt
    26581 tctggagccg ctggtgttca aaccggagga tctacgcctg cagatcgaac gggaactcaa
    26641 atccaagatc attttgcttc gggaagggta tctggaatcc gaagggtcgg cgcggcgcgt
    26701 cagagaactg atcggccggt cactcgtcgc cctggtttcc gtttttaacg ccatgctgta
    26761 tttaaagcag gcaggcctcc cccggcaaaa gaaggaaacg attgaggaag cgggcagggt
    26821 ttttggcgtc gacgcaaacg tctttgcgcg ctgtttcgaa atcaaggcca aagaggacca
    26881 tctttccggc gaagaagtga tcggggtgtt caaaaagtat ttgcgggaag tggaaaaagt
    26941 ctgtcatagg atcgatgggt taaaataatt caacgcgatg gaggagaaaa aaatgtcggc
    27001 aggaaagaaa atcatcattg tggcggcggt cattgcggtt ttgggaattt ctctgtattc
    27061 ccttttcgcg ggcaactaca acaaattcgt ccaaatggac gtcgccatca aggcggcctg
    27121 ggcgcaggtg gaaaatcaac tgcagcgccg ctacgacttg attcccaatc tggtggaaac
    27181 ggtcaaaggc tacgccaagc aggagaagga tgtgctggtg gaagtgacga acgcgcgctc
    27241 gaaagtcgga ggagccggaa cggttcccga caagatcgcg gccaataatg aactctccgg
    27301 cgccctcaac cgtctgatgg tcgtggtgga gcgctacccg gatttgaagt cgaatcagaa
    27361 tttcatgcaa ctgcagactg aactggccgg cacggaaaac cgcattgccg tggaaagaat
    27421 gagatacaac gacgcggtga aaatctacaa tcaggccatc cggacgttcc cggccaatct
    27481 catcgcctct atgttcgggt ttaaggaggc cgctttcttt gaagcgccca gggaagccaa
    27541 agccccgcct caggtcaaat tctgatcccc tattcgtgag gggaacggcg tcaaaccgtt
    27601 cccctcattc ttttcgcttt cttgaagcag cgccggtgaa gacgcgcttt ccgtctattt
    27661 tttgttggag ggcctccaga tctttctctt ttccgcttcc ttgatcaggt cgtctctgaa
    27721 atccggatgg gcgatggaaa tcagtttttc cgcccgatgc caggtggggc aggttttcag
    27781 cgacacgcag ccgtattcgg tgaccacatg gttcaccatc tggcgggtca cggtggtaat
    27841 ggagccgatg tcgaagttcg gcacgatcct ggagatcacc gagccgtctt tgttggtgtg
    27901 ggtggagggc agacagatga agcttcgtcc gcctttggac cagtaggcgc cgagaacaaa
    27961 gtccgacatg ccgccgttgc cggacacctg tttgaatccc gagctttccg cgttcacctg
    28021 gccgtacaga tccacctgca gcgcctggtt gatggagacg aggttgtcga tctggcaaag
    28081 ctgcgtcggg tgattcacgt agccgacgga acaggaggcg atggcgggat tgtggtccat
    28141 ccactcgtat agctttttgt tccccagcgc gaaggtgtaa ttgatttttc ccgtgtcgaa
    28201 tttcttatgt ttgccggtca ttcttccgga ttcgtacatg tcccggtagg cgtccaccag
    28261 catttccgtc cagcccccca ggtctttcaa atccgtctcg ctgatcatct tccccagaat
    28321 gttcggcatg ccgccgatgc ccagctgaat gcagtcgccg tctttcaggt agctcagcac
    28381 attttcggcg attttccggt ccgtgtcggt cggcgaaact tcgggcagtt cggccagcgg
    28441 cgtgttttcg ccttccacga cataggtgac ttcggagata tggattcgct ccatgtttcc
    28501 gccgtagcaa taggggatat tttcgttcac ttccaaaatg caggccttgg ccgtggtggc
    28561 ctgaagatag cagcaggaat tgtgcaggcc ccagttgaag aagccgtctt tatccatcgg
    28621 cgtcacctgg cagaagaaaa catcgcgcag gttcgtgccc agctttgacg ggtccgactg
    28681 agcgtatctc atgtagagct ccgactcgcc gtagagcgtc ggctgataga aaaccccttt
    28741 gcattgctgc tgcaggatgc gggtgagcag actgaagtgc tgatcgttat acgtgaaaac
    28801 ttcaccctgg gggtccttga gcacgacttc aggaaccggc ggaatcgtta ccgccgccat
    28861 gatgttgatg tccttcagct cctccttgcg ggccgcaagc gcacggtcgc agacaaccgg
    28921 tttgccgttg aacatggcga aatcgatcca gtcgcctgat ttgacgatgc gcaccgcctc
    28981 ttcggccgtt cgcagttttt ccttgtacat ttcctgaaaa tttttcaaat tcaaatcctc
    29041 ctgacacaaa atatttggtt catccggttg atgcgtactt tttttgttga aaagatggac
    29101 atgaaccccc aaaagggaat cgacgaagat ttccgatata aggaggggg
//
LOCUS       DAOSRM010000014        28814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1793_length_28814_cov_105.932682, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000014 DAOSRM010000000
VERSION     DAOSRM010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 28814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28814
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1793_length_28814_cov_105.932682"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLA58_02745"
     CDS             complement(<1..231)
                     /locus_tag="PLA58_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR61744.1"
                     /translation="MPLLEISRLTHRFEGLCAVSDFNLRVDPHELVGIIGPNGAGKTT
                     VFNLMTGVYRASEGSINFQGKELVGLNPHSITR"
     gene            complement(218..1255)
                     /locus_tag="PLA58_02750"
     CDS             complement(218..1255)
                     /locus_tag="PLA58_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR61745.1"
                     /translation="MMKAAIVTLQKRWQQLTGLKYFWPVFLAAGLAFCVAADRYDFVD
                     LYLQLIIIYVGINIILTVSLNLINGYMGEFSVGHAGFMAVGAYIASILTVYVFPQNAA
                     GILFPAAILAGGMGAALVGFLVAIPSFKTRGDYLAIVTLAFGMIVKSVLENIDAVGGP
                     RGLLGMEKLTTLPWVFFWTVLSVWVIRNLVYSNFGRGIWSIREDEIASDLMSVNTRQV
                     KIIAFVVSSFFAGVAGGLFAHAIQFINPRMFDILKSTDVLIMVYLGGIGSIAGSVLGA
                     VIYTLLLEILRPLGVWRMVFMPLMLVLLMIYRPRGIMGLREARLFTPLLDRVTEKLWR
                     QRKKEARHAAP"
     gene            complement(1264..2139)
                     /locus_tag="PLA58_02755"
     CDS             complement(1264..2139)
                     /locus_tag="PLA58_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR61746.1"
                     /translation="MAYLFQQALNALQLGSIYALIALGYTMVYGILTMINFAHGDLFM
                     VGAFFCFLLAVYLKLSFVPTLLVSMLGVACLGVVIERLAYKPLRAAPRVSAIITALGV
                     GLFLENFTLALSPYPKHIPPLLTNTTWTIGSLSISSLQVLVIALSLVLMLLLDFVVQR
                     TKAGMAMRAISWDKSIVPLMGVPINKIISFTFAIGTGLGGAAGVMYGLAYPVIDPYMG
                     IMVGWKAFICAVVGGIGNVRGAMIGGFILGAVEILVAAFFPSTYRDFVAFTLLLVLLI
                     LRPYGILGKPQPQKV"
     gene            complement(2256..3422)
                     /locus_tag="PLA58_02760"
     CDS             complement(2256..3422)
                     /locus_tag="PLA58_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR61747.1"
                     /translation="MKHTFILIALLVICSLCVFGCAQKDSDAVRIGVIAELTGDIPAV
                     GASCKNAAELAVREINDNGGILLGEKKYPVELIIEDNAGKADQSASSAQKLITQQKVT
                     AIVGPNASRYALPAAEIAESGKTVLITPWSTAPKATLNSKSNASKKYVFRACFIDPFQ
                     GGVIAKFTLDDLKLKKTAVLYDVASEYNKGIAEIFKEVYEKNGGTIVAFETYTTNDKD
                     FSSQLTKIKKANPDIIFLPNYYSEVPLQIQQAKRLGISVPFIGSDSWGSEELLKLCGK
                     DCEGYYFSTHYAADSASDETKKFIATYKAKYGTTPDDVAALTYDSFGLLAQAIKTAGK
                     NDRQAVRDALAKIPLYNGVTGNMQFKEGSGDPIKSAVILQIKDGKFTWFANAKP"
     gene            complement(3488..4585)
                     /locus_tag="PLA58_02765"
     CDS             complement(3488..4585)
                     /locus_tag="PLA58_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR61748.1"
                     /translation="MEKTDITIIGAGVVGLAVAAELARPGMNVFILEQNARHGEGISS
                     RNSEVIHAGIYYPEGSLKAELCLAGNRMLYESARRRGIPHQKVGKLIVAADPLETGEL
                     ERLFTQGRKNGAAALEMMGGEHIARMEPNIRARAALYSPETGIISAHDLMNDFLARAL
                     ENQAHLICRTRVIRIEQENNAWRILTRNDRGEESTFLSGVVINCAGLASDTVANMMGG
                     AYRLYYCKGDYCGISGVKPGLVRRLIYPAPLKNSVGLGVHLTMDLNGRLKLGPDATYI
                     DRVEDYTVAPEKAALFYEHAVKFLPFLKPENVSADMAGIRPKRQGPKDAFADFAIRAD
                     APGFINLVGIESPGLTASPAIARYVKDLLFH"
     gene            4753..5532
                     /gene="xth"
                     /locus_tag="PLA58_02770"
     CDS             4753..5532
                     /gene="xth"
                     /locus_tag="PLA58_02770"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOR61749.1"
                     /translation="MKIATYNVNSIRSRLHIVLPWLKNHQPDIFCMQETKVEDAKFPL
                     SEFEAAGYTVTFRGGKQYNGVAIASKRKPQKVSFGLADDPPDPDRLAAAHFGDLVILN
                     VYVPQGQEVDTPPFAYKLAWLGRLKKYLFQNFQPDQKLVLCGDFNVAPQQIDVHDPKR
                     ILGHVSFNPEVWKAYENVLTWGLVDVFRKHHPGEPGQYTFFDYRVKDSVGRNLGWRVD
                     HILATNKTAAVSSGCVIDRATRLAEKPSDHAVIYAEWEGKL"
     gene            5532..6107
                     /locus_tag="PLA58_02775"
     CDS             5532..6107
                     /locus_tag="PLA58_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HOR61750.1"
                     /translation="MRKKEDIHRINEDLAARFRKMETELLSAPTIAGLFEALVEGIER
                     EFGVPFVWLTFVDRPGADALTNAAVASDILKSRFSVVSAELLEKIIPDGRQPVLSNQN
                     LQLFYRLMPSSQKYFVRSIAVVPFALEGRMIGAWNNGDADANRYSPDMDTALLAALAD
                     RISLRLTELAAKKNAASAAEKSAEQPGGFHG"
     gene            6100..6450
                     /locus_tag="PLA58_02780"
     CDS             6100..6450
                     /locus_tag="PLA58_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOR61751.1"
                     /translation="MVDEWIKEIKKKCPPDRLGMILVHNGIVRATSKKGAQVARMNLS
                     FDRAALDKAVRRFKEREGIVEIRAWINEGTLNVGDDIMCVCVAGRFRTDVLPVFQELI
                     SLIKTEIVKEDETS"
     gene            6607..7731
                     /locus_tag="PLA58_02785"
     CDS             6607..7731
                     /locus_tag="PLA58_02785"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOR61752.1"
                     /translation="MDTMTEFREVVDQPYAYLQKLKGQTGKKMVGYVCSYTPEEILLA
                     AGAHPVRFFGTKEQIHLADSHLQSYSCSIVRGVLEEGLSGRIGFLDGMIFPHTCDSIQ
                     RLSDIWRMNIPFGFHLDLVLPVKLNTSSARDYMTDVLRKFQNDVGRALGVNITDDDLQ
                     KAIGTMNAIRQSIRDLYEIRSQYPHLLSGADLYTLVRASMMMDRERLAGMLAETAVKM
                     KAKAAGEKPDASLKRIILVGGMCNHPDIYPLIEEAGGAVVWDDFCTGARYFTELVAGG
                     DDPVAGIAERLIRRVVCPAKHAGLDRRAKHILRLVREHNARGVVFLLFKFCDPHAFDY
                     PDIRKSMDEAGIPVLLLEMEDPLPGEGQLKTRFEAFLEMV"
     gene            7743..8981
                     /locus_tag="PLA58_02790"
     CDS             7743..8981
                     /locus_tag="PLA58_02790"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOR61753.1"
                     /translation="MSEVNSDKEKRKIKSEKKMRDIMTAYYIDAKMARQNNKKVAWIT
                     SGGPVEPLIAMDVIPVYPENHGAMIGASKMGVDLCEKAESMGYSNDLCSYARSDISCA
                     TVNGGPIGGLPEPDMLVCCNNICGTVLKWYEVQARYFNIPLFIFDTPFCHTEYTKEAK
                     RYVERQIEEYVAFLEGACGRKMDQDRMKDVGRISFEGQKLWQEVLDTAVHKPSPMTAF
                     DAFFHLALIVTLRGTQQTIDYYQGLLAEMKDRIQAGIGAIPREQYRLLWDNLPIWYRM
                     KWLSQKFAEHDACLVADTYTSAWCGAMKYIDENKFLDSMAEAYSRIYLNIGVDEMAKM
                     VIAMIDKYQVDGFVLHSNRSCKPYSLGQFDIQRIVEKERNIPCLMIEADMVDERSFSE
                     SQVSTRIDAFMEIIKDRKRT"
     gene            9075..9842
                     /locus_tag="PLA58_02795"
     CDS             9075..9842
                     /locus_tag="PLA58_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOR61754.1"
                     /translation="MITAGIDIGSITAKAALVENGKILGAKVTFTGYNAEAAGRKVYE
                     ELLAASGLTASSVGKIVSTGYGRNSVKFADRTFTEIMAHAAGANFLKPEVRTIIDIGG
                     QDSKAIALDERGKVKNFVMNDKCAAGTGRFLEVMARALEVDLDEFGAMSLRSEAPSKI
                     SSLCTVFAESEVISLIARGEQRFDIIAGIHESIAARVSSMIARVGLTEPVMITGGVAR
                     NAGVVAALEKKLGVKIAVLSSAQVNGAIGAAVLAASL"
     gene            10077..10934
                     /locus_tag="PLA58_02800"
     CDS             10077..10934
                     /locus_tag="PLA58_02800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR61755.1"
                     /translation="MMADRESSLTALVTTYIRAAHQLFDAQPLILDDPVALSLLGADA
                     AIQIRQAVERHQTPEGRVLRAHVVLRSRFTEDRLAAAVFRGIKQYVILGAGFDTFAFR
                     QPQWAKKLKIFEVDHPGTQAMKRSKLAAAGIVLPENLVFADVDFEKESLHDGLCRHRV
                     SNAELTFFSWLGVTMYLKEDAIDAVLKTVAAFPESSEIVLTFIQPPTSLSDAEKKSYA
                     RLVESVASAGEPFVSYFEPDALEAKLRNLCFSKMVFLSPADAQAGYFQQRPNDLPAPA
                     VTNIVAAIR"
     gene            complement(10942..13179)
                     /gene="feoB"
                     /locus_tag="PLA58_02805"
     CDS             complement(10942..13179)
                     /gene="feoB"
                     /locus_tag="PLA58_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOR61756.1"
                     /translation="MSSLTVIADLREGDEGVIASMDGGSALTSRLAGMGIVTGARFRI
                     AQVSGGMVVLSVGSTRIALGRGEASKIMARKIDPADDACLPPIEKEISVALVGQPNVG
                     KSTIFNILTGLSQHVGNWPGKTVEKKEGIHRANNVLIRIVDLPGTYSLTSFSEEERVT
                     RDYIIREKPDLVVLVLNAAALERSLYLLSEVLLLDRPVMVAINMLDVASNQGTQIDAK
                     ALQNSLGLPVVPMVAKRNSGIQELVDEMTALGSCPQDFRPELPEVSADHQEIYQKILA
                     AIRPFIREPYTPEWVAVKLMEGDPEVTAMAATQIQPAGHEFIQKLLSEHEDALHAVVN
                     GRYDWIEKMTRAAVSRFKMGQVVLTDRIDHVLTRPVFGIPVLLAIMATVFFLTYSLGV
                     PLQNAMNNGIQSLAQSFEPSLAQWPGWAGGLLIDGVIGGVGSVLSFLPILLIFFAIMA
                     FLEDVGYMARAAFVMDRIMHLVGLHGKSFIPMCLGFGCNVPAVLGARIIETRKARMIT
                     LLLIPFVPCTARLAVLTLISAAIFGQHAALVSWTILALNIAVLGLAGILVNNTIWKQD
                     APFIMELPIYHTPDVRTIGMVVWSRTLSFLRKAGTVILGMSVLIWILSYFPAGTVEES
                     WLAALGKVIEPLGGPLGLDWKMITALLTGLVAKETVVATLGVLYSVGEGGLSAVLPTI
                     MDRASAAAFLVVMMLFIPCVATIAVLRKEMNSNKWFYSTIAMTLIVSYLGGMAAYHFI
                     RWIGL"
     gene            complement(13176..14045)
                     /locus_tag="PLA58_02810"
     CDS             complement(13176..14045)
                     /locus_tag="PLA58_02810"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOR61757.1"
                     /translation="MDITETIDLAHRPFLAEQFHQMHLNISDYTFANVYLFRKVSHYE
                     ILKKECGVFISAYNRQGQNYIMPLADLRVCCLDTLKELLNGRNFFFPIPEEWLPFFPE
                     NEFRATFDESESDYLYRTANLASFAGKPYTRHRNHLNQFFGAYRPEGQPLGRDNFQDA
                     LTVLRRWQDESRFAEGKTDFAVCEEALQNFSSLALWGTLYYIEKQPAGFIIGEALNSD
                     TFCLHFAKASKYYHGIYEFMFNDSAIQLQSRYQYLNLEEDMGNKNLRKTKASYGPERI
                     LKKYRISLKEGIP"
     gene            complement(14082..14768)
                     /locus_tag="PLA58_02815"
     CDS             complement(14082..14768)
                     /locus_tag="PLA58_02815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOR61758.1"
                     /translation="MDIQYSLIRSRKRKKTVSLQIGNRSEITVQAPVHMPAAEIQRFV
                     EEKQAWIEKSLRRQAALQASHPKKAYETGEIFYHLGEACPLEAFFDPLENPGIVFRNN
                     RFFLNCPPNRQMRKYYLTAWYRKQARTYLTRRVDFYSRLLGLTCGGIRITAAEKRWGS
                     CSEKNHLSFAFRLIMAPPQIVDYVVVHEMMHIREKNHSSKFWNLVLEVIPDYRNRRLW
                     LREHQQHFDL"
     gene            14989..15333
                     /locus_tag="PLA58_02820"
     CDS             14989..15333
                     /locus_tag="PLA58_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOR61759.1"
                     /translation="MEEKNLRPWGYYQILSDAADHKVKRILVVPGGTLSLQRHTKRAE
                     HWYAVSGSGDATIDGEVCLLSAGSAVDIPKGATHRLENTGSEDLVIIEVQTGTYFGED
                     DIERLEDKYGRA"
     gene            complement(15347..17080)
                     /locus_tag="PLA58_02825"
     CDS             complement(15347..17080)
                     /locus_tag="PLA58_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOR61760.1"
                     /translation="MFLPTTPEELRKKGWDRPDVLLVTGDAYIDSPHIGAAVIGRVLA
                     AAGYRVGIIAQPDMANDADIRRLGEPRLFWGVTGGCMDSLVANYTALKKKRQGDDLTP
                     GGKNNRRPDRAVIAYANWIRRCFKNTKPIVAGGIEASLRRIAHYDFWSDAVRRSILFD
                     ARIDILAYGMAEKAVLEIAGRLNAGESLRNIRGTCVIENEPPDRYLMLPSFEEAAARP
                     KAFLQMFGVFYANQDPVTARGLVQQHAGRYLAHHPPQAPLSEAELDAVYALPFTRRVH
                     PFYAKQGKVRAMDTIAFSLTTHRGCFGECRFCSIASHEGRTILSRSEESILQEAQKIA
                     ALPDFRGIITDVGGATANMYGIDCARKTSRGACANRHCLDPQPCPSLQNGHSRQIRLL
                     SALRKISGVKKVFVASGIRHDLVLADKRHGASYLRELVKHHISGQLKIAPEHTVPSVL
                     RLMRKPQPEKLKEFKNLFDQMNREEGRKQFLTYYFIAAHPGCAEKDMRELKSFALHEL
                     HATPQQVQIYTPLPSTWSALMYWTGIDPKTGRKIYVEKDLMNKQRQKGIATARRKPPG
                     TEHQRICVKKK"
     gene            complement(17081..17473)
                     /locus_tag="PLA58_02830"
     CDS             complement(17081..17473)
                     /locus_tag="PLA58_02830"
                     /inference="COORDINATES: protein motif:HMM:NF015166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61761.1"
                     /translation="MLIIIHLLLMFAASLCLLAGVGLARFGRKRKFWLRGHKNLNSAG
                     GILLTAGGVMAFVNIILTGSDHLAELHHRIGFIALMLTFLTVFLGFYSLKASNKSVFR
                     AVHRWSGRLSPIAILAALTLGLSMIGIF"
     gene            complement(17525..18364)
                     /locus_tag="PLA58_02835"
     CDS             complement(17525..18364)
                     /locus_tag="PLA58_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR61762.1"
                     /translation="MIIRCWGARGSIPVSGEKYLRYGGNTTCLEIRNNRDDILLVDAG
                     SGIREAGNESLASGRHDFILLLTHAHWDHIMGFPFFKPLYLHQANIDVWGCAFAQDSI
                     REMLARVMSAPYFPINYDAIHANVTYADICKEIYELGGMTITPVALNHPNQGNGYQFS
                     ENGKNFVFLTDNELGFRHEGSLSYENYVAFCQNADLLIHDAEYRNEEYSKRIGWGHST
                     IEQAVKLALDAGVKQLGLFHHNQDRFDTAVDDMVADCRQIIAEKKSPLQCFAVYQGME
                     LSL"
     gene            18505..18705
                     /locus_tag="PLA58_02840"
     CDS             18505..18705
                     /locus_tag="PLA58_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOR61763.1"
                     /translation="MKSVKIAGMTCQHCVMAVTKALGAVEGIKDVRVHLETGIATYDE
                     VKPVDASIIAAAIKKAGYDIVG"
     gene            18650..20941
                     /locus_tag="PLA58_02845"
     CDS             18650..20941
                     /locus_tag="PLA58_02845"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOR61764.1"
                     /translation="MRPSLPRPLRRRVTILSDKVSISVGGMTCAACVRRVENALAQVE
                     GVVEANVNLATARATVVHQARWAGVPALARVITEQGYEFLGETSDAAADPIEASRIRE
                     LGELKLKVSCGAVLSVMIFFGSMQHWFGFLHAIPRQSMLVAMFVLTAPAVFWVGSRFF
                     IGAWKAAKQKTSDMNTLVAVGAFSAYAYSSVATFFPRFFGSAGIAPHVYYDGAAMIIT
                     LVLLGRLLEAVSRGRTSQAIKKLIGLAPKTARVIRDGAEVEMTIDALQTGDEVLVRPG
                     EKVPVDGIILTGRSTLDESMLTGESMPVSREAGQKVFAATMNQSGSFTMRATGVGSDT
                     MLAHIIRMVEEAQGSKAPIQRLADKVASVFVPVVFLIAFLTFGVWYFVPFDANFSRAL
                     LNFVSVLVIACPCALGLATPTAIIVGTGLGAQSGILIKGGEALEKIHKISMVVFDKTG
                     TLTRGEPEATDVVCAVDFTGRQVLAVAAALEKASEHPLAKAIIDRARKDGISPSSTEN
                     FEAFSGMGARGFVDGKAVLVGNRFLMTKEGVSVDLLEGEAVKLAHQGKTLVYVAQGEK
                     IMGLIALADAPKASAKKAVESLKRLGLKVSMVTGDNAGTAASVGAQLGVEQVMAEVLP
                     GDKANTIKQLQAAGEIVAMVGDGINDAPALAAADVGIAVGAGADVAIEAADITLVRDD
                     LNAVPDAVALSAATIRVIRQNLFWAFIYNVIGIPIAAGVLYPFFGILLHPEYAAAAMA
                     MSSVSVVSNSLRLKRVWTKRKNS"
     gene            21133..21684
                     /locus_tag="PLA58_02850"
     CDS             21133..21684
                     /locus_tag="PLA58_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOR61765.1"
                     /translation="MLLKGCLHTHTTCSDGKMSPQEVADAYESKGYDFIAFTDHDYLL
                     KPNYMDLYRQVRTSMIVFYGIELSVFVNGNIHVAKIEGDRERLHIFNHISEYDFTPQQ
                     LLVRLEQLEKMYPLDAVETTKKGFRDAVFESLDIAYPKIASDDSHTLTGIGRAWVELD
                     VKRDKDSIINAIRKGEFWNCYLG"
     gene            21772..22917
                     /locus_tag="PLA58_02855"
     CDS             21772..22917
                     /locus_tag="PLA58_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOR61766.1"
                     /translation="MGKRLAVCAVAQIKNHPDYHYLRFQNMLLECFESIMEQTKVTFD
                     MEKGIRTIISCSDDMFDARTISDNGVTDVMGAHFRGEEKMAQESINGLGYAMACILSG
                     HDDVILFMGHCKESQGESRRMISNLAYDPFYCRPLGMDYHNVDAMQARAYMEKSNVTD
                     EHLAKIVVRSRANGKKNPYGRENKIVSEKDVLNSPMYSDPLREMHIYPVTDWAYGMLL
                     CCEERAKEFTKNPVWITGYGSCMDRYFLGDKDFTSNFSLKNAAKRAYKMAGIKDPKKD
                     IQVFELSDHAAYQLPMWAEGVGIAEEGRGGRWIDDGGMDKFNVNLSGGQLNGNPLLLG
                     GAARAIECYYQLSKQAGERQIKGAKRAVAQAATGGAGQHQAVIVMEA"
     gene            22930..24126
                     /locus_tag="PLA58_02860"
     CDS             22930..24126
                     /locus_tag="PLA58_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61767.1"
                     /translation="MAHKKNRNVGIIGIGQTKHSSHREDVNQPELIHEAVSLALKDAK
                     LTMKDIDCVVHGNMELFEMVHSPDLWHVLGTGAYGKDSIRITTGGTVGATLSCASDNL
                     VASGMYDIVMAIGFEKLQEGHTTGGITNMACPLWFRKIQAGALTGSSAYDVEREFGLE
                     RANRASLMYRIIMDKHACLNPNAHRAFGLDFNQVDDLIRTSPKLVGELKLIEMCSQSD
                     GACCVIYACEKKAKELSKTPVWIRDHITAHKEENFTIFGYDRKYPVLKTHKFAAEHLF
                     QRNGIKDPLNYFDVFEMYDPATWWAIDWIRDFFNLQGDEHLKLIENKEIMIGGKMPIN
                     PSGGVIGSNPIGATALVRVAEAALQVRGNAGPHQIPTPVNHALASGFGGTMWTVMTML
                     EKELNW"
     gene            24137..25327
                     /locus_tag="PLA58_02865"
     CDS             24137..25327
                     /locus_tag="PLA58_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOR61768.1"
                     /translation="MAEYWGVKVEDIFNTMKERFRPEGAKDVDAVFGYDIADEGKWRL
                     TVTNNTMLLEKTDTLAGCVSTMSADGETFVGVNTGKVDATNAFMSGKFKVEGDMGAFG
                     KTGKLFRKFVIPKKEMTTKEYLADMFATIVPRFKAKEAEGLEASFGFDLGGPDGGKWT
                     VFIKDKVCTVTTSIEGKPTVTLEFNDAKDYVDFILGKIDAQSILAAGKASAKGDINMM
                     ASKWPILFEKYKDPMAGAVQEQELLVLKKTISLNEKFSTGPIMGKFLKGLKDKKIFAN
                     KCPECGRLQLPPREVCAECRVRAGEMVEVGPKGEVRYMDIVYYAMPDPLTGVARETPY
                     GSIFILFDGCKGNETLPHFIRKDQIEKIQMGWNEKKGTRVRPVWEENRIGDIWDIQYF
                     EIDE"
     gene            25339..26766
                     /locus_tag="PLA58_02870"
     CDS             25339..26766
                     /locus_tag="PLA58_02870"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOR61769.1"
                     /translation="MTTNSIKGFEDSYQVEGKMALPYSYFAGRVGSKFITTIRDQKKI
                     MGVKCPACNTVYLPPRQVCDVDFTDIRDAWVELESTGTVTNFTVVRYDDKHLPRKAPF
                     VLALIKLDGAGTPFMHILEECKIEDVKIGMKVEAVFAKETTNTILDIDHFKPAAEKAS
                     IHEINAQRKQWVPTEEPEEAAKRKGGKPDMSKPVIITAALTGAATMRNQNPAVPYKPE
                     EFAEEAYKCWKAGAAMVHVHAREDGGMATHDHARIKATYDAIKNKCPDLIVCLSSAVG
                     MGKTAEQRISQIVYVKPEMASLNTNTMNFGIVDRKTGKIFIDYVFENTFGMLQDFAKA
                     MEENGVKPEIECYDMGGLDNTVMIGKQGIFSDPMNFNFVWGVAGGQQFRTEAFVAMMN
                     ALPPKANFTTCGVGTDQYPCIMQSCILGGHMRVGLEDNIRMPNGVLAKGSYEQVEVAV
                     TIANCLGRPVATTEEARLIMGIKKR"
     gene            26833..28683
                     /locus_tag="PLA58_02875"
     CDS             26833..28683
                     /locus_tag="PLA58_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOR61770.1"
                     /translation="MEATEKTMNDVFRNRVQKYKDRLAVEKKMNGVWHSATWNEYYER
                     SRAVGMGLYALGIRKGDMVSILSENRLEWIYTDMGALGIGACVIPIYPTLIADEIEYI
                     LNNSESKVILTEDRGQLKKALEIIDKCPSVEKIIVMEEKDAKGHPSVMSFGTLLELGR
                     KKHTEDPSLFEKLSRDVTVDDLATLVYTSGTTGLPKGAMITHGNVFWIVKSLDAVQPP
                     FADDQDVLVAFLPLSHVFQRIPGHFYAMHRGITISYAQSIDTLLADFEEKRPTMILAV
                     PRVCEKVYQKIIAQVKEQSPFKQKVFHWGQNVGSRISELREKHKPIPLGLQLKYKIAY
                     AVIFKKLQNKLGGRLKWMTAAGAPTAPEIIRFFNAAGITVIQGYGMTETTAPASIQSL
                     ADFRIGTTGKPMPGQDIRIAEDGEILIKGGNVMKGYWKLPEETAAVFTPDGYFMSGDI
                     GKFDEEGNLLITDRKKDLLITSGGKNIAPQMIEGLFKSDPLFTQFIVIGEGKKYLTGL
                     CNIDLEIAAMIADEEKIPYDKPSDLLDNDRFLAVVKKHVEERNTHLAKYETIKDFRII
                     KNEFSQEGGELTATLKLKRKVIYEKYQDLIGDMYGKEAVDELYGQKQSRA"
BASE COUNT         6753 a         7489 c         8404 g         6168 t
ORIGIN      
        1 ccgggtgatc gaatgcgggt tgagccccac cagttccttg ccctgaaagt tgatgctgcc
       61 ttcgcttgcc cgatacacgc cggtcatcag gttgaagacg gtggttttcc ctgcgccgtt
      121 ggggccgatg atgccgacca gttcatgggg atcaacccgc aggttgaaat cggataccgc
      181 gcaaagtccc tcgaaccggt gtgtgagccg ggagatttca aggagcggca tggcgcgcct
      241 cctttttcct ttgccgccag agcttttccg tgaccctatc gagaagcggt gtgaacaggc
      301 gggcttcccg caggcccatg atgccgcgcg gccggtaaat catcagaagc accagcatca
      361 gcggcatgaa gaccattcgc cagacgccca gcgggcgcag gatttccaga agaagtgtgt
      421 agatgaccgc gccgagaacc gatccggcga tggagccgat gccgcccagg taaaccatga
      481 tcagtacatc ggtcgatttc agtatgtcga acatgcgcgg gttgatgaac tgaatcgcgt
      541 gggcgaaaag gcctccggcg acgcccgcaa aaaaggacga tacgacaaag gcgatgattt
      601 tgacctggcg cgtgttgacg ctcatcagat cgctggcgat ttcatcttcc cggatcgacc
      661 aaatgcctcg cccgaaattg gaatacacca ggttgcggat aacccagacg gacagcactg
      721 tccagaagaa aacccaggga agcgtcgtga gcttctccat gcccagaagc ccccggggcc
      781 cgcccacggc gtcgatgttt tccaggacgg acttgacgat catgccgaaa gcaagcgtga
      841 cgatggccag gtaatcgcct cgtgttttaa aggagggaat ggccaccaga aagccgacca
      901 gcgccgcgcc catgccgccg gccagaatcg ccgcgggaaa caaaatgccg gcggcgttct
      961 gcgggaaaac atacacggtc aagatcgagg caatataggc gccgacggcc atgaatcccg
     1021 cgtgtcccac ggagaattct cccatgtagc cgttgatcag attcaggctg accgtgagaa
     1081 tgatgttgat gcccacgtag atgatgatca actgcaggta taaatccacg aaatcgtagc
     1141 ggtcggcggc aacgcagaag gccagccccg cggccaaaaa gaccggccag aaatatttca
     1201 ggccggtgag ctgttgccag cgcttttgca atgtgacaat tgcggctttc atcatcgtca
     1261 tgcctatact ttttgcggct ggggcttgcc cagaatgccg tagggccgca gaatcagcag
     1321 aaccagaagc agcgtgaaag cgacaaagtc ccggtacgtg gacgggaaaa acgccgccac
     1381 gaggatttcc accgcgccca gaatgaaacc gccgatcatg gcgccgcgca cgttgccgat
     1441 gccgcccact acggcgcaga tgaaggcttt ccagccgacc atgatgccca tgtagggatc
     1501 gatcaccgga taggccagtc cgtacatgac gccggctgcg ccgcccagtc ccgtgccgat
     1561 ggcgaaggtg aaggagatga ttttattgat cggcacgccc atcaggggaa caatcgactt
     1621 gtcccaggaa atggcgcgca tggccatgcc cgctttggtg cgctggacca caaaatccag
     1681 aagcagcatc aaaaccagcg aaagggcgat caccagcacc tgcaaggaag agatggacaa
     1741 ggatccgatg gtccaggtgg tgttggtgag cagcggcgga atgtgcttgg ggtaggggga
     1801 aagggccagt gtgaaatttt ccagaaacaa ccccacgccc agcgcggtga tgatggcgga
     1861 gacgcgcggc gcggcccgca ggggcttgta ggccagtctt tcgatgacga cgcccagaca
     1921 ggccacgccc agcatggaga caagcagcgt cgggacaaac gacagcttca gataaacggc
     1981 cagcagaaag cagaaaaacg ctcccaccat gaacagatcg ccgtgggcga agttgatcat
     2041 cgtcaggatg ccgtaaacca tcgtgtagcc cagggcgatg agcgcgtaga tgctgcccag
     2101 ctgcagggcg tttaacgctt gctgaaaaag ataggccatg ccagatcctt ccccgccccc
     2161 tgcgggtggg gatcgaggag agaggggttt aatttgggtc acccatccat gatccctccc
     2221 atcgggggat catggatgaa ggatgaaaat actttctatg gtttcgcgtt tgcgaaccat
     2281 gtgaactttc cgtctttgat ttgcagaatg acggcacttt tgatcggatc gccggagcct
     2341 tccttgaact gcatattgcc cgtcacgccg ttgtaaagcg gaattttcgc gagcgcgtcg
     2401 cggacggcct ggcggtcgtt cttgccggcg gttttgatgg cctgcgccag gagcccgaat
     2461 gaatcgtaag tcagggcggc cacgtcgtcc ggcgttgttc cgtatttggc tttgtaagtc
     2521 gcgatgaatt ttttggtttc gtcgctggcc gagtccgccg cgtagtgcgt gctgaagtag
     2581 tagccttcgc agtctttgcc gcagagcttg agcagctctt cggaccccca ggaatccgag
     2641 ccgataaacg gaaccgaaat gcccagacgc ttggcctgct ggatttgcag aggcacttcg
     2701 ctgtagtagt tgggcaggaa gataatgtcg ggattggctt ttttgatttt ggtgagctgc
     2761 gatgaaaaat ccttgtcgtt ggtggtatag gtttcgaaag cgacaatcgt gccgccgttt
     2821 ttctcgtaaa cctccttgaa aatctcggcg atgcctttat tatattcgga ggccacatca
     2881 tacagcaccg ccgttttttt cagcttcagg tcatccaggg tgaatttggc gattacgcct
     2941 ccctgaaacg ggtcgatgaa gcaggcgcga aaaacgtatt ttttggaggc gttgcttttg
     3001 gaattcagcg tggctttggg cgcggtggac cagggagtaa tcaaaacggt tttgcccgac
     3061 tcggcgattt ccgccgccgg cagcgcgtag cggctggcgt tggggccgac aatggccgtc
     3121 actttctgct gggtgatcag cttctgcgcg gacgaggcgg actggtccgc tttgccggca
     3181 ttgtcttcaa tgatcagctc caccggatat ttcttctccc ccagaaggat gccgccgttg
     3241 tcgttgattt cccggacggc gagctccgct gcgtttttgc aggaggcgcc caccgccgga
     3301 atgtcgccgg tcagctcggc gatgacgccg atccttaccg cgtcggaatc cttttgggcg
     3361 cagccaaaaa cacaaaggga gcaaatcacc aaaagagcaa tcaatataaa agtgtgtttc
     3421 atggattttc tcctgcctgg catcagaaaa ttttgacccc agcttataag ggaaggacgc
     3481 gtcagtgtca atggaaaaga aggtctttga cataccgggc gatagccggc gaggccgtga
     3541 gccccggtga ttcgatgccg acgaggttga tgaaaccggg agcgtcggcc cggatggcaa
     3601 aatcagcgaa agcgtctttg ggcccctggc gtttgggacg gatgccggcc atgtcggccg
     3661 aaacgttctc cggcttcaaa aaaggcagaa atttcacggc gtgctcatag aataaagcgg
     3721 ctttttcggg ggcgacggtg taatcctcca cccggtcaat gtaagtggcg tccggtccca
     3781 gcttcagtcg tccgttcaag tccatcgtta aatgcacacc cagccccaca ctgttcttaa
     3841 gcggggcggg gtagatcaac cgccgaacga ggcccggctt cacgccggaa ataccgcagt
     3901 aatcgccttt gcagtaatac agccgatagg cgccgcccat catgttggcg accgtatcgg
     3961 atgcgagccc cgcgcagttg atcaccaccc ccgacaggaa cgtggattcc tccccccggt
     4021 cgtttcgggt gaggatccgc caggcgttgt tctcctgttc aatacggatc acgcgggtgc
     4081 ggcagatcag atgggcctgg ttctccagcg cccgggccag gaaatcattc atcaggtcat
     4141 gggcgctgat aatgcccgtt tccggcgagt acagggcggc gcgggccctg atgttcggct
     4201 ccatccgggc gatgtgctct ccgcccatca tttccagggc ggcagcgccg ttttttcggc
     4261 cctgggtgaa gagacgctcc agttcgccgg tttccaacgg atcggcggcc acaataagtt
     4321 tgccgacttt ttgatgggga atgccgcgcc ggcgggccga ttcatacagc atccggtttc
     4381 cagccaagca cagttcggcc ttcagcgatc cttccggata gtagatgcct gcgtgaatca
     4441 cttcactgtt gcgcgagctg atgccctctc cgtgccgcgc gttctgctcc aaaataaaga
     4501 cattcatgcc cgggcgcgcc aattccgctg cgacggccag gccgacaacc cccgctccaa
     4561 tgatcgtgat atcggttttt tccatattcc ctttgcccat gtttgagaag aaggggttgc
     4621 aaccccttgc ttcctcatcg ctgacgatga tcaagaacca tacacgattt cggcgggcgg
     4681 cgcaaaaaat cttgagcgac gcggcaggtc gtggtaataa ccaccgaaat ttacagaaag
     4741 gtcttttaac cattgaagat tgccacatac aatgtcaatt cgatccgttc ccgtctgcat
     4801 atcgttttgc cttggctcaa aaaccaccag ccggatattt tctgcatgca ggaaaccaag
     4861 gtcgaggacg ccaaatttcc cctctctgaa tttgaagcgg cgggatacac agtaaccttc
     4921 cgcggcggca agcaatacaa tggcgtggcg atcgcttcaa aacgaaaacc gcagaaggtt
     4981 tcctttggac tggccgatga tcctccggat cccgaccgcc tggccgccgc gcatttcggt
     5041 gatctggtga ttttaaacgt ctacgttccg caggggcagg aagtggacac gcctccgttt
     5101 gcctataagc tcgcgtggct ggggaggctc aaaaaatatc ttttccaaaa ctttcagccg
     5161 gatcagaaac ttgtgctgtg cggtgatttt aacgtcgcgc cccagcagat tgacgttcac
     5221 gatcccaaac gtattctggg acatgtctct ttcaatccgg aggtctggaa ggcgtacgaa
     5281 aatgttttga cctggggtct ggttgatgtt tttcggaagc atcatcccgg tgagccgggg
     5341 caatacactt tttttgacta ccgcgtgaaa gattcggtcg gacgaaacct gggctggcgc
     5401 gtggatcata ttctggccac gaataaaaca gccgcggtgt cgagtggctg cgtcattgac
     5461 cgggccacgc gtcttgcgga aaagccgtcc gatcacgcgg tgatttacgc cgagtgggaa
     5521 gggaaactct gatgaggaaa aaagaggaca tccatcgcat caatgaagat cttgcggcgc
     5581 gtttcaggaa aatggaaacg gaactcttat ccgcccccac cattgccggg cttttcgaag
     5641 cgttggtgga agggatcgaa agagaattcg gcgttccttt tgtctggctg acgtttgtgg
     5701 atcggccggg ggcggatgct ttgacgaatg ccgccgtcgc gtcggacatt ttgaaaagca
     5761 ggttcagcgt cgtttccgcc gagcttctgg aaaaaataat tcccgacgga cggcagcccg
     5821 ttctctccaa tcaaaatctc cagctttttt acaggctgat gccgtccagt caaaaatatt
     5881 ttgtgcggtc gatcgccgtg gttccttttg cgctggaggg acgaatgatc ggagcctgga
     5941 acaacggcga tgcggacgcg aaccgctatt cgccggatat ggataccgcg ctgcttgctg
     6001 cgctggccga ccggatttcc cttcggctga cggagctggc cgcgaaaaaa aacgccgcat
     6061 ccgccgcaga gaaaagcgcg gagcagccgg gaggttttca tggttgacga gtggattaaa
     6121 gaaatcaaga aaaagtgccc gccggacagg ctgggcatga ttctggtgca caacggcatt
     6181 gtgcgggcca cgtccaaaaa aggcgctcag gtggcgcgca tgaatctttc ctttgaccgg
     6241 gccgcgctgg ataaagcggt gcgtcgcttt aaagaaaggg aaggcatcgt ggagattcgc
     6301 gcctggatta acgaagggac gctcaacgtc ggcgacgaca tcatgtgcgt ctgcgtggcg
     6361 ggccggttcc gtacggacgt tctgccggtg tttcaggagc tcatttcgct gatcaaaaca
     6421 gaaatcgtga aggaagatga gacttcatga acgaagtcct ttttcaaggt tagtggcgca
     6481 aagaagaaca gcggcaggaa tttcgtgttc gggaagcgaa gaaacattgc acttttgatc
     6541 ccgaatatgc tagcttgcgt tcacaaaacg ggaaagccgc agatttcggg ctgggaagga
     6601 aatgcgatgg acaccatgac ggaattcagg gaagttgttg atcaacctta cgcgtacctg
     6661 caaaaactca aaggccaaac cggaaagaag atggtgggct atgtctgttc ttatacgccg
     6721 gaggagatcc tcctggcagc cggggcccat ccggtgcgtt ttttcggcac caaagagcag
     6781 attcatctgg ccgattccca tcttcagtcc tacagctgct cgattgtccg cggcgttctg
     6841 gaggagggcc tttccgggag aatcggattt ctggacggta tgattttccc tcatacctgc
     6901 gattccattc aaaggctgtc cgacatctgg cgcatgaaca tcccgttcgg atttcatctg
     6961 gatctggtgt tgcccgtcaa gctcaacaca tcctccgcac gggactatat gacggatgtc
     7021 cttagaaaat tccaaaacga cgttggccgc gcgcttgggg tgaacattac cgatgacgac
     7081 ctgcaaaaag caatcgggac gatgaatgcc atccggcaat cgatcaggga tctttacgag
     7141 attcgcagcc aataccccca tctcctgtcc ggcgcggatt tatacacact ggtccgggcg
     7201 tccatgatga tggaccggga gcgcctcgcg gggatgcttg ccgaaacggc tgtgaagatg
     7261 aaggcaaaag cggccgggga aaagccggac gcctctctga agaggattat tcttgtcggc
     7321 ggcatgtgta atcatccgga tatttatccc ctgattgaag aggcaggcgg agccgttgtc
     7381 tgggatgatt tctgtacagg ggcaagatat tttaccgagc tggttgccgg gggggatgat
     7441 cctgtagcgg gtatcgcgga gcggctgatt cggcgtgttg tttgcccggc caagcatgcc
     7501 ggattggacc ggcgcgccaa acacatcctc cgcctcgtgc gggagcataa cgcccggggc
     7561 gtcgtttttc tgcttttcaa attctgcgat ccgcacgcgt ttgattatcc cgacattcgg
     7621 aaaagcatgg atgaagcggg aattcccgtt ctgcttttgg aaatggaaga cccccttccc
     7681 ggggaagggc agctgaaaac acgctttgaa gcatttctgg aaatggtgtg aaaggagatg
     7741 tcatgtccga agtaaactct gataaggaaa aaaggaaaat caaatccgaa aagaaaatgc
     7801 gggatatcat gacggcctat tatatcgacg ccaaaatggc ccggcagaac aacaagaagg
     7861 tggcctggat cacgagcggc gggccggtgg agccgctgat cgccatggat gtgattcccg
     7921 tctatccgga gaatcacgga gccatgatcg gcgccagcaa gatgggcgtc gatttgtgcg
     7981 agaaagccga atcgatgggc tattccaacg atctgtgttc ctatgcccgt tccgacatct
     8041 cctgcgcgac ggttaacggc ggtccgatcg gagggctgcc cgaaccggac atgctggtgt
     8101 gctgcaacaa catctgcggg accgtgctga aatggtacga ggtgcaggcg cgctatttca
     8161 acattcccct gtttattttc gacacgccct tctgccacac cgaatacacg aaagaggcga
     8221 agcgctacgt cgagcggcaa attgaggagt atgttgcctt cctggagggt gcctgcggcc
     8281 gtaaaatgga ccaggaccgc atgaaggacg tcggcagaat ctcctttgaa ggccagaaat
     8341 tgtggcagga agtcctggat acggccgtcc acaagccctc tcccatgacg gccttcgacg
     8401 ctttttttca cctggcgctt atcgtgacgc tccggggcac gcagcagacg atcgattatt
     8461 accagggcct gctggccgag atgaaagaca gaattcaggc cggcatcggc gccattcccc
     8521 gggaacaata ccgcctcctg tgggacaatc ttccgatttg gtaccggatg aaatggctgt
     8581 cgcagaaatt cgccgagcac gatgcgtgtc tggtggccga cacttacacc tcggcctggt
     8641 gcggcgcgat gaagtacatc gacgaaaaca aatttctgga ttcgatggcc gaagcctact
     8701 cgcgcattta cctgaacatc ggcgtggacg aaatggcgaa aatggtcatc gcgatgatcg
     8761 acaaatatca ggtggacggg tttgtgctgc attccaaccg cagctgcaaa ccctattcgc
     8821 tgggccagtt cgatattcag cggatcgtcg aaaaagagcg gaacattccc tgcctgatga
     8881 tcgaagcgga catggtcgat gagcgaagtt tttcggaaag ccaggtttcg acgagaatcg
     8941 acgcttttat ggagatcatc aaggatagga aaaggacgta aaagcccgca ggtttcagcg
     9001 ttggaaaagg gctttcacgg cggcaggaaa cgctttcctg aaaaagacga acgacgaagc
     9061 acaagaaatg ggaaatgata acggcaggca ttgacatcgg gtccatcacg gcaaaagcgg
     9121 ctctggttga aaacgggaaa attctgggcg cgaaggtgac gtttaccggc tataatgcgg
     9181 aggcggcagg cagaaaggtt tatgaagaat tgctggccgc ttccggcctc acggcgtcat
     9241 ccgtgggtaa aattgtatcc acgggctacg ggcgcaattc ggtgaaattc gccgacagga
     9301 cattcacgga aatcatggcg catgccgccg gcgcgaattt tctgaagccg gaggtccgca
     9361 ccatcatcga cattggcgga caggacagca aagccatcgc gctcgatgag cggggcaagg
     9421 tcaaaaattt tgtcatgaac gacaagtgcg ccgcgggaac cggccgtttt ctggaggtca
     9481 tggcgcgcgc gctcgaagtc gatttggatg aattcggcgc catgtcactt cggtcggagg
     9541 cgccctccaa aatcagcagt ctgtgcacgg tcttcgcgga atcggaagtc atttccctga
     9601 ttgcccgcgg cgagcagaga ttcgacatca ttgccggcat ccatgaatcg atcgcggcgc
     9661 gcgtctcctc catgattgcg cgcgtcggcc tgaccgaacc ggtcatgatc accggcggtg
     9721 tcgcgcgcaa cgccggcgtc gtggccgcgc tggaaaagaa gctcggcgtg aaaatcgccg
     9781 tattgtcctc cgcgcaggtc aacggcgcga tcggcgccgc ggttctggct gcatcgctct
     9841 aatgccgtca aggcgcaaaa gaattgcggc tgttggcaaa gatgtcagtg tgagagcttt
     9901 gaaaaatgtc atttcgagga gcgtgagcga cgagaaatct taaagacggc cgtttgttca
     9961 agatttctcc cttcggtcga aatgacaaaa atggccgtta ttcaaagctc tcagtgtgcc
    10021 tttgaatacc gcacgccata acaggttcgc cgccttgcct cctgaaggag acaaccatga
    10081 tggcagaccg agaatccagt ctcaccgctc tggtaaccac atacatccgg gctgcgcatc
    10141 agttattcga cgctcaaccc ctcattcttg acgatcccgt tgccctatct cttttgggag
    10201 cggatgccgc aatacaaatc aggcaggcgg tagaacgtca tcagacgcca gaaggacggg
    10261 tattgcgagc gcacgttgtt ctgcgctcac gctttacgga agaccggctc gccgcagcgg
    10321 tgtttcgcgg aatcaagcag tatgtcatcc tgggggcggg attcgatacc tttgcgttca
    10381 ggcagccgca atgggcgaaa aaactgaaaa ttttcgaagt tgaccaccct ggaacacagg
    10441 cgatgaagcg ttccaaactg gcggctgcgg gaatcgtgct gccggaaaat cttgtttttg
    10501 ccgacgtcga ttttgaaaag gaatccctcc atgacggact ttgccggcac cgcgtgtcga
    10561 atgccgagct gactttcttt tcgtggttgg gcgtgacgat gtatctcaaa gaagacgcca
    10621 ttgatgctgt gctgaaaacg gtcgcggcgt ttccggaatc cagcgagatt gtgctgactt
    10681 ttatccagcc gccgacgtcc ttgtcggatg cggaaaagaa gtcctatgca cgacttgttg
    10741 agagcgtagc aagtgcgggc gagcctttcg tgagttattt cgagcctgac gcactggaag
    10801 caaaactgcg aaacctgtgt ttttcgaaaa tggtttttct ctcgccggcc gatgcgcagg
    10861 ccggatactt tcagcagcgc ccaaatgacc tgccggcgcc ggcggtcacc aacattgtcg
    10921 ctgccatacg ataatcctga attacaggcc gatccaccgg ataaaatgat aggccgccat
    10981 tccgcccagg taggagacga tcagggtcat tgcgatcgtg gagtaaaacc acttgttgct
    11041 gttcatttcc ttgcgcagga cggcgatcgt cgcgacgcag ggaataaaga gcatcatcac
    11101 gaccagaaac gcggccgcgg aggcgcggtc catgatggtc ggcagaacgg ccgatagccc
    11161 cccctcgccc acggaataca gcacgcccag cgtggcgacc acggtttcct tggcgacgag
    11221 tccggtgagc agcgcggtga tcatcttcca gtccagcccc agcggcccgc ccagcggttc
    11281 aatgactttt cccagggcgg ccagccagct ttcctccacc gttccggcgg ggaaatacga
    11341 aagaatccag atcagcaccg acatccccag aatcacggtg cccgccttgc gcaggaagga
    11401 aagggttctc gaccagacga ccatgccgat ggtccggaca tccggcgtgt ggtaaatggg
    11461 cagctccatg ataaacgggg cgtcctgctt ccagatcgtg ttgttgacca gaataccggc
    11521 gagtcccagc acggcgatgt tgagggccag gatcgtccaa gaaaccaggg cggcgtgttg
    11581 accgaagatg gcggccgata tcagcgtgag caccgccagc ctggcggtgc aggggacaaa
    11641 cgggatgagc agcagcgtga tcatgcgggc tttgcgcgtc tcgatgatcc gcgcgccgag
    11701 cacggcggga acattgcagc cgaaacccag gcacatggga atgaagcttt ttccgtgcag
    11761 tcccaccagg tgcatgatgc ggtccatgac gaacgcggcg cgcgccatgt agcctacgtc
    11821 ttccagaaac gccatgatcg cgaaaaagat cagcaaaatg ggaagaaaac tcaacacgga
    11881 gcccacgccg ccgatcacac cgtcgatgag aagccccccg gcccagccgg gccattgggc
    11941 gagcgacggt tcgaaagact gggcaaggga ttggatgccg ttgttcatgg cgttttgcag
    12001 cggcacgccg agggagtagg tcagaaaaaa gacggttgcc atgatggcca gcagaaccgg
    12061 gatgccgaaa accgggcggg tcagcacatg gtcgatgcgg tcggtgagca ccacctgtcc
    12121 cattttaaag cgtgagaccg ccgcgcgggt cattttctca atccaatcgt aacgcccatt
    12181 gacgacggcg tgaagggcgt cttcatgctc gctgagcagt ttctgaatga actcatggcc
    12241 ggccggttgg atttgtgttg ccgccatggc cgttacttcc gggtcgcctt ccatcaattt
    12301 taccgccacc cattcgggcg tgtagggctc ccggataaaa ggcctgatgg cagccagaat
    12361 cttttggtaa atttcctgat ggtcggcgga gacctcgggc aactcgggcc gaaaatcctg
    12421 agggcaggaa cccagggctg tcatctcatc caccagctcc tgaatcccgc tgtttcgttt
    12481 ggctaccatg ggcacaaccg ggagtcccag tgaattttgc agcgctttgg cgtcaatctg
    12541 cgtgccctga ttggatgcga catccagcat gttgatggcc accatcacgg gacggtccag
    12601 aagcagcacc tcggagagaa gatacaggct tcgctccagc gccgcggcgt tgagcaccag
    12661 cacgacgagg tcgggcttct cccggatgat ataatcgcgc gtgacccgtt cttcctcgga
    12721 aaaagaggtc aggctgtagg tgcccggcag atcgacgatc cggattaaaa cgttgttggc
    12781 gcggtggatg ccttcttttt tttcgaccgt tttcccgggc cagttgccga catgctggga
    12841 aagtccggtc agaatgttaa agatcgtgga tttgccgacg ttgggctggc cgaccagggc
    12901 gacggaaatt tctttttcga tcggggggag gcaggcatca tcggcgggat cgattttgcg
    12961 cgccatgatc tttgacgctt cgccccgtcc caaagcaatc cgggtggatc caacggagag
    13021 gacgaccata ccgccgctga cctgcgcgat gcggaaccgg gcgccggtga cgatgcccat
    13081 gccggccagg cggctggtca gcgccgagcc gccgtccatt gaagcaatga cgccttcatc
    13141 gccttcccgc aaatcagcaa tgaccgtcag actgctcatg ggatgccttc cttgaggctt
    13201 atgcgatatt tttttaaaat ccgttccggg ccgtacgacg ctttcgtttt ccgcaggttt
    13261 ttattgccca tgtcctcttc cagattcagg tattgatagc gggactgcaa ttggatggcc
    13321 gaatcattga acatgaactc ataaatgccg tgatagtatt tggaggcttt ggcaaagtgc
    13381 aggcaaaacg tgtcgctgtt caaagcctct ccgatgataa agccggccgg ttgtttttct
    13441 atgtaataca gggttcccca aagcgcaagc gaggagaaat tttgcagagc ctcttcgcat
    13501 accgcaaaat cggttttgcc ctcggcgaac cgggattcat cctgccagcg ccgcaggacc
    13561 gtcagggcgt cctgaaaatt gtcccgaccc agcggttgac cctccggacg gtacgcgcca
    13621 aagaactgat tcagatggtt gcggtggcgg gtgtagggct tgccggcaaa ggacgccagg
    13681 tttgccgtcc ggtagagata atccgattcg ctctcgtcga aggttgcacg gaattcattt
    13741 tcggggaaaa aaggcagcca ttcctcaggg atcggaaaga aaaagttgcg tccgttcagc
    13801 agctccttca gcgtatccag acagcagacg cggagatcgg caagcggcat aatgtagttt
    13861 tgtccctgcc ggttgtaagc ggaaatgaaa acgccgcatt cttttttcag gatttcataa
    13921 tgactgacct tgcggaacag ataaacattg gcgaacgtgt agtccgaaat attcaaatgc
    13981 atctgatgaa actgctccgc cagaaatggc cggtgcgcca gatcgatggt ttcagtgatg
    14041 tccatagaat aaaatggttt aagccctttc cgattctttg ctcaaagatc aaaatgctgc
    14101 tgatgttcac ggagccaaag ccttcggttt cgataatcgg gaattacttc tagcactaag
    14161 ttccagaatt ttgaagaatg atttttctcc cggatatgca tcatttcatg aacaaccaca
    14221 taatccacta tttgcggcgg cgccatgatc aggcgaaaag cgaaggacaa atgatttttt
    14281 tcagagcagg atccccaccg cttttcggcg gcggtaatcc gaatgccgcc gcaggtgagg
    14341 ccgagcaggc ggctgtagaa atccacccgc cgggtgaggt aggtccgtgc ctgtttccgg
    14401 taccaggccg tcaggtaata ttttctcatt tgccggttcg gggggcagtt cagaaaaaag
    14461 cggttgttcc ggaatacaat ccctggattt tccagcgggt caaaaaaggc ttccagggga
    14521 caggcttcgc ccagatgata aaaaatttct cctgtttcat aggctttctt cggatgcgag
    14581 gcctgcagcg ctgcctgccg gcgcagggat ttttcaatcc aggcttgttt ttcttcgaca
    14641 aagcgctgaa tctccgcggc cggcatatga accggcgcct gaaccgtgat ttccgaacga
    14701 ttgccgattt gcagcgagac ggtttttttt ctttttctgc ttcgtatcaa cgaatattga
    14761 atatccatgg cttttatgcg gaaaagcgtt aacacattca gaagggaaaa gcgaggctgg
    14821 aagtgcgggt tagcggtttt gccgcgcgca gggtcgtcgc ggctgttccc ttcccgtaag
    14881 cggcccgaaa ggaacaaacg ggaggcgcgg cgcagccggc gggcaaagat gcttttcatt
    14941 catcggcgat tgtgataggc aatcgtcaaa aatcctggaa gtgaaaaaat ggaagagaaa
    15001 aatcttcgtc cctggggtta ctatcaaatc ctgtccgatg ccgcggatca caaggtcaaa
    15061 agaattctgg tcgtgccggg cggcaccctg agcctgcagc gccacaccaa gcgggcggag
    15121 cactggtacg cggtttcggg cagcggcgac gccacaattg acggcgaggt ttgtctcctt
    15181 tccgccggca gcgccgtgga tattccaaaa ggggcgacgc accgcctgga aaacaccggc
    15241 agcgaagacc tggtgatcat cgaagtgcag accgggactt atttcggtga ggatgatatc
    15301 gagaggctgg aagacaagta cggcagggcg tgaagaagcg gaaggactac ttctttttca
    15361 cgcaaatcct ctgatgttct gtgcccggtg gtttccgccg ggcggtggcg atgccctttt
    15421 gtctctgttt attcatcaaa tctttttcca cataaatctt tctgccggtt ttcggatcga
    15481 ttcccgtcca gtacatcaac gccgaccagg tggaaggaag cggtgtgtaa atctgcacct
    15541 gctgcggcgt cgcatgcaat tcatgaaggg caaaggattt cagctcccgc atgtcttttt
    15601 cggcgcagcc cggatgcgcg gcgatgaaat agtaggtaag aaactgcttt cgcccttcct
    15661 ccctgttcat ttggtcaaaa agattcttga actccttcag cttttccggc tggggttttc
    15721 gcatcaggcg cagcacggaa ggcacggtat gctccggcgc aatctttaat tggccggaaa
    15781 tgtgatgctt caccagttcg cgcagatagg acgcgccatg tcttttatcc gccaaaacca
    15841 gatcatgccg gatgccggac gcaacgaaga cttttttgac gccggaaatt ttacgcagag
    15901 ccgaaagcag acggatttga cgggagtggc cgttttgcag agacgggcag ggctgcggat
    15961 cgagacaatg tctgttcgcg caggcgcccc ggctggtttt tctcgcacag tcgattccat
    16021 acatattggc cgtcgcgccg ccgacatcgg tgatgattcc cctgaagtcc ggcagcgcgg
    16081 ctattttttg cgcttcctgc agaatggatt cttccgaccg gctgagaatg gtccggccct
    16141 cgtgggaggc gatggagcaa aaacggcatt cgccaaagca cccgcggtgc gtcgtcaggg
    16201 agaaggcgat ggtgtccatg gcgcggacct tgccctgttt tgcataaaaa ggatgaacgc
    16261 gccgcgtaaa gggaagagcg taaactgcgt ccagttcagc ttcggacaga ggcgcctggg
    16321 gcggatgatg cgccagataa cggccggcgt gttgctgaac caaaccgcgg gccgtcaccg
    16381 ggtcctgatt cgcgtaaaac acaccgaaca tttgaaggaa agctttcggt cgggctgccg
    16441 cttcttcgaa agagggcagc atcagatacc tgtcgggcgg ttcgttttcg ataacgcagg
    16501 ttccgcggat gttccggagg gattctccgg cgttcaggcg tccggcgatt tccaggaccg
    16561 ccttttccgc catcccgtaa gccagaatgt cgatgcgcgc gtcaaacaaa atggagcggc
    16621 ggacggcatc cgaccagaaa tcataatgcg cgatccgccg cagactggcc tcgatgccgc
    16681 cggcgacaat cggctttgtg tttttgaaac agcggcggat ccaattggcg taggcaatga
    16741 cggcccggtc gggtcggcgg ttatttttcc caccgggtgt caggtcgtcg ccttgcctct
    16801 tttttttcag cgccgtgtaa ttggcgacga gcgaatccat gcagccgccg gtgacgcccc
    16861 agaacaggcg aggttctccc agacggcgga tgtccgcatc gttcgccatg tccggttgtg
    16921 cgatgatgcc cacccggtag cctgcggctg ccagcacccg gccgatcacc gccgcgccga
    16981 tatgagggct gtcgatatag gcgtcaccgg tgaccagcag gacgtccggg cggtcccaac
    17041 cctttttccg aagttcttca ggtgtggtcg gcagaaacat ttaaaagatt ccgatcatgg
    17101 agagccccag ggtcagcgcg gcaaggattg cgattgggga aaggcggccc gaccagcggt
    17161 gaacggctcg aaagacgctt ttgttggagg cttttaaaga atagaacccg aggaaaaccg
    17221 tcaggaatgt cagcatcaag gcgatgaacc cgatccggtg atggagttcc gccagatgat
    17281 ctgagcccgt caggatgata ttgacaaaag ccataacgcc gcccgcggtc aacaggatgc
    17341 ctccggcgct gttgagattt ttgtgtcccc tcagccagaa ttttcttttc cttccgaacc
    17401 tggccaggcc gacgcccgcc agaaggcata aagacgccgc aaacatcagc aacagatgaa
    17461 taatgatcag catggcatgc ggcctcctcg cgccggggga ttcgggtgtt tttctgaaac
    17521 aagactacaa ggacaattcc attccctgat aaacggcaaa gcattgcaga ggggattttt
    17581 tttcggcgat gatttgccgg cagtcggcga ccatgtcgtc gacggccgtg tcgaatcggt
    17641 cctggttgtg atggaaaagc cccagttgtt tgacgccggc atccagcgca agtttcaccg
    17701 cctgttcgat ggtggagtgc ccccagccga tccttttgga atactcttca ttccggtatt
    17761 cagcgtcgtg aatcagtaaa tcggcgtttt ggcaaaaagc aacatagttt tcataggaca
    17821 gcgatccttc gtgccggaat cccagttcgt tgtcggtcag aaaaacaaag ttttttccgt
    17881 tctctgaaaa ttgataaccg tttccctgat tggggtggtt gagcgcgacc ggtgtaatgg
    17941 tcataccgcc cagttcgtag atttccttgc agatgtccgc ataggttaca ttggcatgga
    18001 tggcgtcata attgatgggg aagtacggag cggacatgac gcgcgccagc atttcccgga
    18061 tggagtcctg agcgaacgcg cagccccaga cgtcgatatt ggcttgatgc aggtaaagcg
    18121 gtttgaaaaa agggaatccc ataatatggt cccagtgcgc atgggtcaaa agcaggataa
    18181 aatcgtgccg gccgctggcc agggactcat ttcctgcttc gcggatgccg gatccggcgt
    18241 caaccagcag aatatcgtcg cggttgttcc ggatttccag gcaagtcgtg ttgcctccgt
    18301 aacgaaggta tttctcgccg gataccggta tggaacctct cgcgccccag caacgtatga
    18361 tcatcaatcg tcccctgtcg gcataatcat acagaatatt tatttttttt caagcaggcg
    18421 atcataaaaa atacggcttt cccccttgct attgaaccaa tatccattat cttagggaaa
    18481 aacgaaaatc atcggaggtt tcttatgaaa agcgttaaaa ttgcaggcat gacttgtcag
    18541 cattgtgtca tggcggttac aaaagcgctg ggtgccgtgg agggaattaa agatgtcagg
    18601 gttcatttgg aaacgggaat cgcaacttac gatgaagtaa agcccgtgga tgcgtccatc
    18661 attgccgcgg ccattaagaa ggcgggttac gatattgtcg gataaagtat ctatttcagt
    18721 gggcggcatg acctgtgcag catgcgtgcg ccgtgtggaa aacgcgcttg cacaggtaga
    18781 aggcgtggtc gaggcgaatg tcaatctggc cactgcccgc gcgaccgtgg tccatcaggc
    18841 ccgctgggcg ggagtgcccg cactggcccg cgttatcacc gaacagggat acgaattcct
    18901 gggggagaca tccgatgcgg ccgccgatcc gatcgaggct tcccgcatcc gggagcttgg
    18961 cgagttgaag ctgaaagtct cctgcggcgc tgtgttgagc gtcatgatct ttttcggctc
    19021 gatgcagcac tggtttgggt ttttgcatgc gattccgcgt cagtcgatgc tcgtggcaat
    19081 gtttgtttta acggcgcccg cggttttttg ggtggggagc cggtttttca ttggagcctg
    19141 gaaggccgcg aagcaaaaaa cgtcggacat gaacacgctg gtggcggttg gcgcattctc
    19201 cgcttacgcc tattcgtccg tcgccacttt ttttccccgt ttctttggca gtgcgggcat
    19261 cgctccccat gtgtattatg acggcgcagc catgatcatc acgctggtgc tgctgggccg
    19321 gcttctggag gctgtcagca ggggaaggac ttcccaggcc attaagaaac tgatcgggtt
    19381 ggcgccgaaa acggcgcgtg tcattcggga cggcgcggaa gtggaaatga ccattgatgc
    19441 actgcagacc ggagacgagg tgctggtgcg gccgggagaa aaagttccgg tggacggaat
    19501 catcctgacg ggccgatcga cgctggatga gtccatgctg accggcgaga gtatgccggt
    19561 ttccagggag gccggccaaa aagtgtttgc cgccaccatg aatcaatcag gaagcttcac
    19621 gatgcgcgcc accggcgtag gctcggacac catgctggcg catattatcc gtatggtgga
    19681 agaagcgcag ggttccaagg cgccgattca gcgcctggcg gacaaagtag cgtctgtttt
    19741 tgtgccggtt gtctttttga ttgcctttct gacgtttggt gtttggtatt ttgttccctt
    19801 cgacgccaat ttcagccgcg ctctgcttaa ttttgtgtcg gtgctggtca tcgcctgccc
    19861 ctgcgcgctg gggctggcca cgcccacggc catcatcgtg ggtacgggcc ttggcgcgca
    19921 aagcggcatc ctgatcaaag gcggcgaggc ccttgaaaag atccataaaa tttccatggt
    19981 cgtatttgac aaaaccggaa cgctgacgcg tggtgaaccg gaagcaacgg atgtggtctg
    20041 cgcagtggat tttaccgggc ggcaggtgct tgccgtggcc gccgcgttgg agaaagcgtc
    20101 ggagcatccg ctcgcaaaag cgattattga tcgggcgcga aaggacggca tttcgccgtc
    20161 ttccacagaa aactttgaag cgttctccgg catgggcgcc agaggctttg ttgacgggaa
    20221 ggcggtgctg gtcggaaacc gtttcctgat gacgaaggag ggggtttcgg ttgatctcct
    20281 ggaaggcgag gcggtcaagc tggctcatca gggaaaaacc ctggtgtatg ttgctcaagg
    20341 ggaaaaaatc atgggcctca ttgcgctggc cgatgcgccg aaagcttccg caaaaaaagc
    20401 cgtggaaagc ctgaagcgtc ttggtttgaa ggtgtcgatg gtgaccggcg acaacgcagg
    20461 aacggcggcg agcgtcggcg cacaactggg cgttgaacag gtcatggcgg aggtgctgcc
    20521 gggcgataag gcaaatacca tcaaacaact tcaggccgcg ggcgaaatag tggccatggt
    20581 gggcgacggc atcaatgacg cgccggcgct ggcggcagcc gatgtcggga ttgccgttgg
    20641 agcgggtgcg gatgtggcca ttgaggcggc ggacatcacg ctggtacggg atgatttgaa
    20701 tgccgtaccg gatgctgtcg ccctttcggc agcgacgata cgggtcattc gtcagaatct
    20761 gttttgggcg tttatttata atgtcatcgg cattccgatt gccgcgggcg tcctttatcc
    20821 tttcttcgga atcctgttgc atccggagta tgccgccgcc gcaatggcca tgagctccgt
    20881 ttccgttgtg agcaattccc tgcgattgaa aagggtttgg acaaagcgaa aaaactccta
    20941 acaaattcca tcatctccaa ttatttttac aaaaataaaa ttttttcttg acaatcagaa
    21001 acggggagag tattcggata gccattggtt gaacgtccaa tcaaatatta ggttgggcgt
    21061 ccaataaaaa gctggcgtat catcaaaacc cgcttcaggt aaatttttac gggaattttc
    21121 tggttggtga cgatgctctt aaaaggctgt ttacatacgc acaccacatg ctcggacggt
    21181 aaaatgtcgc ctcaggaagt cgccgacgcc tatgaatcca aaggatacga ttttattgcc
    21241 ttcacggatc acgattacct gttgaagccg aactatatgg atctttacag gcaggtcagg
    21301 accagcatga ttgtttttta cggcatcgag ctttcggttt ttgtcaacgg aaatatacat
    21361 gtcgcgaaaa ttgaaggcga ccgggagcgg cttcacatct ttaatcatat cagcgaatat
    21421 gattttaccc cgcagcaact gctggttcgc ctggagcagc tcgaaaagat gtatcccctg
    21481 gatgccgtgg aaaccacgaa gaagggattt cgcgatgcgg ttttcgaatc actggacatc
    21541 gcctacccta aaatcgcgtc tgatgattcg cataccctca cgggaatcgg cagagcgtgg
    21601 gttgaactgg acgtaaaacg ggataaggat tccattatca acgcgatacg gaagggagaa
    21661 ttttggaact gctacctggg ttgaggtcgg gtggctgttt cagcggacgg cacgcatctg
    21721 tttgaaggat gaaaaatatt caattcgaac caacaggaag gaggacgggt aatgggtaag
    21781 agattggctg tctgcgcggt ggcgcagatc aagaatcatc cagattatca ttatttgcgt
    21841 tttcagaaca tgctgctcga atgttttgag tcgatcatgg agcagacgaa ggtcactttt
    21901 gacatggaaa agggcatccg gacgatcatc agttgttcgg atgacatgtt cgacgcccgg
    21961 acgatatccg acaacggcgt cacggatgtt atgggcgcgc attttcgcgg cgaggaaaaa
    22021 atggctcagg aaagcatcaa cggcctcgga tacgccatgg cctgcatcct ctccggccat
    22081 gatgatgtga ttcttttcat gggacactgc aaagaatcgc agggggaaag ccgccggatg
    22141 atttccaacc tggcctatga tcccttctac tgtcggccgc tcggcatgga ctatcacaat
    22201 gtggatgcga tgcaggcccg cgcctacatg gaaaaatcga acgtgacgga cgaacacctg
    22261 gcgaagatcg tggtccgctc ccgagccaac gggaagaaaa acccctacgg ccgcgaaaat
    22321 aaaatcgtca gtgaaaaaga cgtgctgaat tcgccgatgt attcggatcc gcttcgggaa
    22381 atgcacatct atccggttac cgactgggct tacggaatgc tgctgtgctg cgaagagcgg
    22441 gcaaaggagt tcacgaagaa ccctgtgtgg atcaccggat acggcagttg catggatcgc
    22501 tatttcctgg gcgacaagga tttcacgtcg aatttttcgc tcaagaatgc cgcaaagcgc
    22561 gcatataaaa tggccggtat caaagatccc aagaaagaca tccaggtgtt tgaactgagc
    22621 gaccatgcgg cgtatcagct tccgatgtgg gccgaaggcg tgggcattgc tgaagaagga
    22681 cggggcggcc ggtggatcga cgatggcggc atggacaaat tcaatgtgaa cctgtccggc
    22741 ggacagctca acggaaatcc gcttctcctg ggaggagcgg ccagggctat tgaatgttac
    22801 taccagttgt cgaaacaggc gggtgagagg cagatcaaag gcgcgaaacg ggcagtggcc
    22861 caggcggcga ccggcggggc cggacagcat caggccgtaa tcgtcatgga agcgtaggga
    22921 ggttgaatca tggcacataa aaaaaacaga aatgttggaa ttatcggcat cggacagacg
    22981 aagcattcca gtcatcgcga ggatgtcaac cagcctgaat tgatccatga agcggtcagc
    23041 ctggcgctga aagatgcgaa gctgaccatg aaagacattg actgcgtcgt tcatggcaat
    23101 atggagcttt ttgagatggt ccactcgccg gatctctggc acgtgctggg aaccggcgcc
    23161 tacggcaagg attccattcg aattaccacg ggggggaccg tcggggcaac cctttcctgc
    23221 gcgtcggaca atctcgtggc ttcaggcatg tatgatatcg tcatggccat cgggtttgag
    23281 aaactgcagg aaggccacac cacgggcggc atcaccaaca tggcctgtcc tctgtggttc
    23341 agaaagatcc aggcgggggc gcttaccggt tcctcggcgt acgatgtcga gcgggaattc
    23401 ggtctggaac gggcgaaccg cgcgtcgctc atgtaccgga tcatcatgga caagcacgct
    23461 tgcctcaatc ccaatgcgca ccgggctttt ggtctggact tcaatcaggt tgacgatctg
    23521 atccggactt cgccgaaact cgtcggcgaa ctgaaactga tcgaaatgtg ttcccagtcg
    23581 gacggcgcct gctgcgtgat ctatgcctgc gagaaaaaag cgaaagagtt gtccaagacc
    23641 ccggtctgga tccgcgacca catcaccgcc cacaaggaag agaatttcac gatcttcggc
    23701 tatgaccgga agtatcctgt cctgaagacg cataaatttg cggcagagca tcttttccaa
    23761 cgcaacggca taaaagatcc cttaaattat tttgacgtat tcgaaatgta tgatccggcg
    23821 acctggtggg ccattgactg gattcgggat ttcttcaatc tgcaggggga cgagcacctc
    23881 aagctcatcg aaaacaagga aatcatgatc ggcggcaaaa tgcccatcaa tccgtccggc
    23941 ggcgtgatcg gatccaaccc gatcggcgcc acggcgctgg tgcgggtggc cgaggctgcc
    24001 cttcaggtgc gcggcaatgc cggtccgcat cagataccga ccccggtcaa tcacgcgctc
    24061 gcgtcagggt ttggcggcac catgtggacg gtgatgacaa tgctcgaaaa ggaacttaac
    24121 tggtaggagg acgatcatgg cagaatactg gggagtaaaa gtagaagata tattcaatac
    24181 gatgaaggag cgtttccgtc ccgagggggc caaggatgtc gatgccgttt ttgggtatga
    24241 cattgccgac gagggcaaat ggagactgac cgttacaaat aacacgatgt tgctggaaaa
    24301 gaccgacacg ctggccggat gcgtgtcgac catgtcggcc gacggggaga cctttgtcgg
    24361 ggtcaacacg ggcaaagtcg atgcgaccaa cgccttcatg agcggaaaat ttaaggtgga
    24421 aggcgatatg ggcgctttcg ggaagaccgg caagcttttc cgcaaatttg tcattcccaa
    24481 aaaggagatg acgaccaaag agtatctggc cgacatgttt gccaccattg tcccgcgttt
    24541 caaggcaaaa gaagccgaag gcctcgaagc ttcttttggc ttcgacctcg gcggaccgga
    24601 cggcggcaag tggaccgtct tcatcaagga caaggtttgc acggtcacca cgtccattga
    24661 gggaaagccg acggtcacgc tggaattcaa cgacgccaaa gattatgttg atttcattct
    24721 cggcaagatc gacgcgcaga gcattctggc cgcgggaaaa gcgtccgcga aaggcgacat
    24781 caacatgatg gcgtcgaaat ggcccatcct cttcgaaaaa tataaagatc ccatggccgg
    24841 cgctgttcag gaacaggaac tgctggttct caaaaagacc atatcgttga acgagaagtt
    24901 ttcaaccggt cccatcatgg gcaaatttct caaagggctg aaagacaaaa aaatattcgc
    24961 gaacaaatgt cccgaatgcg gccgcctgca gctgccgccg cgggaagttt gcgccgagtg
    25021 ccgtgtgcgc gccggcgaga tggtcgaggt cgggcccaag ggggaagtcc ggtatatgga
    25081 catcgtttac tacgccatgc cggatccgtt gaccggtgtg gccagggaaa ccccttacgg
    25141 gtcgatattc atcctttttg acggctgtaa gggcaacgaa accctgccgc actttatacg
    25201 gaaggatcag atcgaaaaga ttcagatggg ctggaacgaa aagaagggga cccgcgttcg
    25261 tcctgtctgg gaagaaaacc ggatcggcga catctgggat attcaatatt tcgagatcga
    25321 tgagtaagga gacgggaaat gacaacaaat tcaattaaag gattcgaaga cagctatcaa
    25381 gtcgaaggca aaatggcgct cccttattct tactttgccg gccgggtggg cagcaagttc
    25441 attacgacca tccgggacca gaagaaaatc atgggagtca aatgccccgc ctgtaacacg
    25501 gtctatctgc cgccgcggca ggtctgcgac gtggatttta ccgacattcg cgacgcgtgg
    25561 gtcgagctgg aaagcactgg cacggtgacg aacttcacgg tggtgcgcta tgacgacaag
    25621 cacctgccgc gcaaggcgcc gtttgtcctg gccctcataa aactagacgg cgccggcacg
    25681 ccctttatgc acattctgga ggaatgcaaa atcgaggatg tcaaaatcgg aatgaaggtc
    25741 gaggcggtgt tcgcgaagga aacaacgaat accatcctcg atatcgatca tttcaagccc
    25801 gcggctgaaa aagcgtccat tcatgaaatt aacgcacaga gaaaacaatg ggttccgacc
    25861 gaggaacccg aggaggcagc aaaaagaaaa ggagggaaac ccgatatgtc caaacccgtc
    25921 attattaccg cagcgcttac cggcgccgcc acgatgagaa atcagaatcc ggccgttccg
    25981 tacaaaccgg aggaattcgc tgaagaagcc tacaagtgct ggaaggccgg cgcagccatg
    26041 gtccacgtcc atgcccggga agacggtggc atggccaccc atgatcacgc gaggatcaag
    26101 gcgacgtatg acgccatcaa gaacaagtgt ccggacctta ttgtctgttt gagctcggcc
    26161 gtcggtatgg gaaaaacggc ggagcagcgg atctcccaga tcgtttatgt caaacccgaa
    26221 atggcgtcac tcaacaccaa caccatgaac ttcggcattg tggaccgcaa aacggggaaa
    26281 atctttattg actatgtttt tgaaaacacc ttcggcatgc tgcaggactt cgccaaggcg
    26341 atggaagaaa acggcgtgaa gccggaaatc gagtgctacg acatgggcgg tctggacaat
    26401 accgtcatga tcggcaagca gggcatcttc agcgatccaa tgaacttcaa cttcgtgtgg
    26461 ggcgttgcgg gcggtcagca gttccggacc gaagccttcg ttgccatgat gaacgcgctt
    26521 cccccgaaag cgaacttcac cacctgcggc gtcgggacgg atcaatatcc ctgcatcatg
    26581 cagtcctgca tcctcggcgg acacatgagg gtgggcctgg aggacaacat ccgaatgccg
    26641 aacggagtgc tggccaaggg gagttacgaa caggtggagg tggccgtgac catcgccaat
    26701 tgtctgggac gtccggtggc aaccacggag gaggcgcgcc tgattatggg gattaagaag
    26761 cgataaacga tcggcgacgc tgcggatgac gtcatcggca gcgtcgcctc aaattcattc
    26821 gggagacgct cgatggaagc aacagaaaaa accatgaacg atgtttttag gaaccgcgtt
    26881 caaaaataca aagaccgtct tgcggttgaa aagaagatga acggcgtgtg gcatagcgcg
    26941 acctggaatg agtattacga gcggtcgcgc gccgttggca tgggacttta tgccctgggg
    27001 attcgaaaag gggacatggt ttccatcctt tccgaaaacc ggttggaatg gatttacacc
    27061 gatatgggcg cgctgggaat cggcgcctgc gtgattccga tctatcctac gctgattgcg
    27121 gatgagatcg agtatattct caataattcc gagtccaagg tcattctgac ggaagaccgg
    27181 ggccagctca aaaaagcgct ggaaatcatc gacaagtgcc ccagtgttga aaagatcatc
    27241 gtgatggagg aaaaggacgc caaagggcat cccagcgtca tgagcttcgg gactctgctg
    27301 gaattgggaa gaaaaaaaca caccgaagac ccctccctct ttgagaaact ttcccgggat
    27361 gtgacggtcg atgatctggc caccctcgtt tatacctccg gcacaaccgg attgcccaag
    27421 ggcgccatga tcacccacgg aaacgttttc tggattgtga aatccttgga cgcggttcaa
    27481 cctccttttg ccgacgatca ggatgtcctg gtggcctttc ttcccctctc gcatgtgttt
    27541 caacggattc ccggccattt ttacgccatg caccgcggaa ttaccatttc ctatgcccag
    27601 tcgatcgaca cgcttctcgc ggacttcgaa gagaagcggc cgaccatgat tctggccgtt
    27661 ccgcgcgtat gcgagaaggt ttatcagaag atcatcgcac aggtgaagga acagtccccc
    27721 tttaagcaga aggtgttcca ctgggggcag aacgtcggca gccggatcag cgaacttcgc
    27781 gagaaacaca aacccattcc actcggcctg caattaaaat acaaaatcgc ctatgccgtc
    27841 atcttcaaaa agctccagaa caagctgggc ggacggctga aatggatgac ggccgccggc
    27901 gcgcccacgg cgccggaaat tatccggttc ttcaacgccg ccgggatcac cgtcatccag
    27961 ggatacggca tgacggaaac cacggcgccg gcgtccatcc aatcccttgc ggattttcgc
    28021 atcggcacga ccggcaagcc gatgcccggt caggacatca ggatcgccga agacggagag
    28081 attctgatca agggcggaaa cgtgatgaag ggatactgga aactgcccga ggagaccgca
    28141 gccgttttca cgccggacgg atatttcatg tcgggcgaca tcgggaagtt cgatgaggaa
    28201 ggcaatctcc tcatcacgga ccgcaagaag gatctcctca tcacctccgg aggcaaaaac
    28261 atcgctccgc agatgatcga agggctgttc aaatccgatc cgctgtttac ccagtttatc
    28321 gtgatcggag aggggaaaaa atatttgacc ggattgtgca atatcgacct ggaaattgcc
    28381 gcgatgatcg ccgacgagga aaaaattccg tatgacaaac cgagtgattt gctggacaac
    28441 gatcgattcc tggccgtcgt aaaaaaacac gtcgaggagc gcaatacgca cctcgccaag
    28501 tatgaaacca tcaaggattt cagaattatc aaaaatgaat tttcgcagga aggcggcgaa
    28561 ctgacggcga cgctcaaact gaagcgaaaa gtcatttatg agaagtatca ggatctgatc
    28621 ggcgacatgt acgggaaaga ggccgtcgat gaactgtacg ggcaaaagca gtcccgggcg
    28681 tgagccgaca ggcggtgatg aaaaccttag tcaagaaggt ggaaaactat gaataaagaa
    28741 gtggaattga aacaggggca gaatcaactt ttttccgatc cggatgcgga caaagcgagg
    28801 gagtttttcc gcag
//
LOCUS       DAOSRM010000015        27906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1893_length_27906_cov_112.465980, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000015 DAOSRM010000000
VERSION     DAOSRM010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 27906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27906
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1893_length_27906_cov_112.465980"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(506..802)
                     /locus_tag="PLA58_02880"
     CDS             complement(506..802)
                     /locus_tag="PLA58_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOR61771.1"
                     /translation="MKIIWSPLAIDRASEIAEYIALDKPAAADKWITTIFSKVEQLAS
                     SPEIGRVVPEIEDEQFRELIYGNYRIVYRIEKKQVSILTIRHGKQILPIEEITA"
     gene            complement(799..1080)
                     /locus_tag="PLA58_02885"
     CDS             complement(799..1080)
                     /locus_tag="PLA58_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOR61772.1"
                     /translation="MQRLKIDQDIKPLSEVRIGIATYIKQVRDTKRPVIITQHGKGVA
                     VLLDASEYENMQERLELLTDVQTSLGQLANGQGITHEDAKNKVLRRVHK"
     gene            complement(1234..3066)
                     /gene="uvrC"
                     /locus_tag="PLA58_02890"
     CDS             complement(1234..3066)
                     /gene="uvrC"
                     /locus_tag="PLA58_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOR61773.1"
                     /translation="MDENTALKEKMTSAPRCPGVYMMQDASGKVIYVGKANDLKNRIS
                     SYLTGRDTRPMAPFLMARASDIAFITTATEKEALILENNLIKKHRPRYNVILRDDKTY
                     YHLSLDPAETFPRLRLVRKRQNDAALYFGPYPSGLAAKETLRFIQRIFPLRTCRDRDF
                     QLRARPCLEYQIGRCLAPCKNRIDEAAYRTLVENAVSFLRGRRRELISDLKKQMEQAA
                     QELNFEEAARLRNRIGALSHALEQQHVDGGSETDQDVLGVFSDSETHRICILFVRAGK
                     LTGSKSFTPVKTKADREEILSSALRQHYDEAGLPEEILLPYSLPDEAVLLEWLAEKQQ
                     KRVRITVPRRGAKKALVDLACANARELQASDRRKEEQKEAALLFLQEKLMLAALPRRI
                     ECFDISNLGGKSAVGSMVVFQDGEPEKSGYRRFRVRGPDEPDDYGMMREVLTRRFQGP
                     DPAPDLLVVDGGKGQLNVALAVLSDLKIKLDVIGLAKEERSFLAAKRRLQGKPAKSED
                     RVYVPRRKDAIYLSSSPAALALLQRLRDEAHRFAVSYHRKVKTKNDLSSVLDAIPDIG
                     PSRRTLLLKYFGSAQQVRKASLEDLQKVPGIGRELAEKIYSALS"
     gene            complement(3316..4167)
                     /locus_tag="PLA58_02895"
     CDS             complement(3316..4167)
                     /locus_tag="PLA58_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOR61774.1"
                     /translation="MAIPYHALIYPEDRQSNRASREARGRAAFAVIAHRGASAYYPEN
                     TMPAFRAAIDMRADMVELDVQLTADGEVVVFHDENISRCTDGRGRIADYPFIRLRKLD
                     AGGWFHKKFTGERIPALTEVLALCKDRIAVNIEVKTEAVTDAVSGGIEERCLKIVDET
                     GMREHVVYSSFDSRAILHFKQIDALTPVAVLYEKKQHGLRLPSEIVQCLGADAFNCSL
                     RELGEKWLPDLKLHGIPVHIYTVNDEKNMRRLLDLGIDGIFTNRPDVLRSVWEEYKGS
                     CVMIDGQ"
     gene            4323..4898
                     /locus_tag="PLA58_02900"
     CDS             4323..4898
                     /locus_tag="PLA58_02900"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOR61775.1"
                     /translation="MKVLGIMGSPRVGGNSDVLLGQALEGAKAAGADVKKIILARKEI
                     AGCRDCKKCNKTGICALKDDMAEIQEQILEAGAVIHSVPVYFWTMTSQMKAYLERWCV
                     FFDAEWKWQKAYYPKMKGKRIGLITVCGDPDPHTADPIVHSFRMTAEMTRLNWLGCVM
                     ASATDKGEIAGNEAALSEAFDLGKKAAAPVS"
     gene            complement(4951..6000)
                     /locus_tag="PLA58_02905"
     CDS             complement(4951..6000)
                     /locus_tag="PLA58_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOR61776.1"
                     /translation="MKKSPAGMPVRSKKKKLNLALQGGGAHGAFTWGVLDRLLDEERL
                     DIEGIVGTSAGAVNATILAYGLASGGRKKAQELLNHFWITNSKNSQCSPFQPTVFDKL
                     LGSRGSIEFSPYWQMFDVLSRMFSPYQWNPNNKNALKEMLEELIDFSVLQKSRSIRLF
                     ICATNVLTGRLKVFENREITPDMIMASACLPYLTQAPEIEGQYYWDGGYMGNPPIFPV
                     IYDTDCRDVLIVQINPINIPEVPRTTNEIFDRINTLSFNSSLMREMRAIYFITSLIEK
                     GELDSSKHKHTFIHTIDAEEVMAKLSASSKMNLDMEFLQYLFNIGREKAGEFLTNHFD
                     KIGRQSSTDLVAKFF"
     gene            complement(6418..9075)
                     /gene="polA"
                     /locus_tag="PLA58_02910"
     CDS             complement(6418..9075)
                     /gene="polA"
                     /locus_tag="PLA58_02910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOR61777.1"
                     /translation="MTEKNPNSLLAVVDGSNYLYRAFYGIPALSNSRGFPTNAIYGFT
                     IMLMKLLKDIHPDYIVVTFDLKGPTTRHEAYEDYKATRKPMPEDLIAQVPLIKEVIRG
                     FSIPILEKQGTEADDLIGTLVVRARKKGWRAVIISGDKDLMQLIGDGVTLVDTMKDKT
                     YDSAAVREKFGVGPDKVVEILGLMGDASDNIPGVPGIGPKTARRLIEEYGSVEAVLEN
                     AEKLHNVKLRESFRKYADQARLSRRLAVISTDVDLDFDLKDAARREPDREALSRLFRD
                     FEFSSLLQQISAGPAKPEVPCKIVADTKALAELVGALRGVRTFACEMFPEKNTPQALI
                     GMAIGAGRERFYIPLGHDGAGQQLKTEEVWPALMPFWANPKITKYAYDLKTALVLLPD
                     LPVSAGMDLQLAAYLLNPARHSYTLDEVAWEYLQERVPSMAEAAGGRGKNQSLAPAPV
                     DQAALSAGQRVAALLSLAEKLLAGLDQAGASSLYRDVEMPLLFTLAGMEKKGVLVDAA
                     LLKQMSGELSQLLALSEEKIYRLAGETFNINSPKQLQMILFEKMQLPKGKKTKEGYST
                     DVDVLSDLARSHELPAEILAYRSLAKLKSTYVDALPALISPQTGRIHTSYNQTVAATG
                     RLSSSNPNLQNIPVRTVEGKRIRQAFIAPPGCVLISADYSQIELRVLAHLSEDEALLD
                     AFAADADIHTRTASDVFGVFPEMVSPDMRRQAKVINFGILYGMSAFGLARELGVSQKT
                     AQVYIDGYFARYKKVRAYLDGVLEGARRDGFVCTILNRRRWLPDLQSKIPSVRQFAER
                     MAINAPIQGSAADLIKVAMVNIDRLLKEKNLDARLIMQVHDELVLEAPVQEQEDVKKL
                     VREEMENVIRLKVPLKVEIASGVNWDEAH"
     gene            complement(9088..9534)
                     /locus_tag="PLA58_02915"
     CDS             complement(9088..9534)
                     /locus_tag="PLA58_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HOR61778.1"
                     /translation="MDAIDKKLLNLLQKEFPLEEEPFRLIGERCGISEEETLARVQKL
                     KDRGIIRRIGAVFDGKKLGRASTLCAARVPEGQIRSFVRIVSAEKGVTHNYRRAHEYN
                     IWFTATAASAEELEALLSDVKKKTGVTDILDLRAVRTFKIDASFDL"
     gene            complement(9611..10636)
                     /gene="ahbD"
                     /locus_tag="PLA58_02920"
     CDS             complement(9611..10636)
                     /gene="ahbD"
                     /locus_tag="PLA58_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HOR61779.1"
                     /translation="MIAWEVTRSCNLNCVHCRAASHLGPYPGELSTGECLRLIDDIAR
                     FSQPVIILTGGEPLLRQDIFDLAAYGTQKSLRMVMATNGTLVDEAKVRKMIDSGIQRV
                     SISIDGPDAQSHDALRQQPGAFAGALAGIAAMKAAGMEFQINTTITTANLHQIKAIHD
                     LALRIGAAAHHIFLLVPTGRGRDLAEQAITAADYEETLRWFYRESLTCAIQLKATCAP
                     HYFRVMHQNKRKGENPAKKVGGHFHESTRGCLGGISFCFISHVGQVQPCGYLELDCGN
                     IRKQSFGEIWETSEVFRNLRDYESYKGKCGRCEFIKVCGGCRARAYEATGDYRAEEPL
                     CLYQPRQ"
     gene            complement(10801..11775)
                     /gene="hemB"
                     /locus_tag="PLA58_02925"
     CDS             complement(10801..11775)
                     /gene="hemB"
                     /locus_tag="PLA58_02925"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOR61780.1"
                     /translation="MQFPEYRGRRLRKNENFRRLVRETRLSADELVYPLFAVPGRDVK
                     KPIASMPGQFQMSVDHLAKEAREAHALGIPALLLFGIPARKDEQGTGAFAKDGIVQQA
                     VKRVKNEVPDMLVITDVCLCEYTSHGHCGIIEKGEVLNDATLEVLAETALSQVKAGAD
                     MVAPSAMMDGQIAAIREILDENALENIPIMAYSAKYASSFYGPFREAAESAPQFGDRK
                     AYQMDPANSDEAIREISLDVNEGADIIMVKPALPYLDIICRARQEFDLPLAAYNVSGE
                     YSMIKAAAKLGWLDEEKAMLESVTAIKRAGADILITYWAKDLAKVLAT"
     gene            complement(11779..12966)
                     /gene="ahbC"
                     /locus_tag="PLA58_02930"
     CDS             complement(11779..12966)
                     /gene="ahbC"
                     /locus_tag="PLA58_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HOR61781.1"
                     /translation="MIGISKLYCGAVEPSDVLRYNRHSGRLPSHLLQFSADKKPVVVW
                     NMTRRCNLKCIHCYSSSADIDYPDELTTEEGKGMIDDLAAFGSPVLLFSGGEPLMRKD
                     LLELARYATDMGMRAVISTNGTLITKEIAAELKKIGLSYVGVSLDGLQPTHDRFRGRK
                     GSFGKAMEGIRNCREAGIKVGLRFTVNRHNVADVPAMFDLLRKEKIERMCFYHLVYSG
                     RGSKLREEDLTHGEARRLLDLIADKTKAMFDDGLAPEILTVDNHADGPYLYLKMREKD
                     PARAAEILELLEMNEGNSTGVGIGCISWDGEVYPDQFWRNRPLGNVRKRPFSEIWTDE
                     KNEFLMKLKDKKKYVTGRCAACVWLSVCGGNFRARAEAEGDEWGPDPACYLTDEEISS
                     PKE"
     gene            complement(12963..14525)
                     /gene="cobA"
                     /locus_tag="PLA58_02935"
     CDS             complement(12963..14525)
                     /gene="cobA"
                     /locus_tag="PLA58_02935"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOR61782.1"
                     /translation="MKKAKPGKVYIIGAGPGDPGLITIKAARCLGLADVVVYDNLVNE
                     DLLAYAPAGARVIYAGKEGGNHTLTQDKINELLVREALRGHTVARLKGGDPFIFGRGG
                     EEAEELAARHIPFEVIPGVTSAIAVPAYAGIPLTHRGVTSTVAFITGHEDPTKEKSDI
                     DWHALAGIGTLVFLMGVKNLDQITGALIAQGKPPETPAALIRRGTTPDQKVLSGTLAA
                     IADLAAENRFKPPAILVVGKVVELRDTLGWFDTKPLFGLGVAITRPERQADDLAKLLA
                     AEGARPIAFPTIAIEPPSDWSELDAALAGLDAYQWLVFTSANGVRFFFERMQQKGKDV
                     RDLKGVKICCIGPATARQLADRGIRADLVPEEFIAEGILKAFAAMDIQGMNILIPRAE
                     KARDILPRTLKAQGARVTVATAYRTVNSGRKKEELARRLAAGDVDVITFTSSSTVTNF
                     FDIMGKDFTLPPSVRVACIGPVTAATAKQAGLSVDIAQEEYTMEGLVAALYRAFQKSA
                     PGTGGGAERKEG"
     gene            complement(14527..15441)
                     /gene="hemC"
                     /locus_tag="PLA58_02940"
     CDS             complement(14527..15441)
                     /gene="hemC"
                     /locus_tag="PLA58_02940"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOR61783.1"
                     /translation="MKIKVGTRGSKLALAQTNSAVAGLKKAAPEIEAEICVIRTSGDI
                     MQDVSLTQIGGQGVFVKEIEEALLSGQIDLAVHSMKDVPGETPEGLVFAAILPREDVR
                     DVLVTRGRIKFEYLPKGAKIGTGSQRRGAQLKSFMPDLEIVPLRGNIDTRLKKIETEN
                     LTGVVLAAAGVKRLGYGEVVTQFLPTEIMLPAVGQGALGLQVRKSDAELAGLCARLNH
                     AVTEAEVTAERAYLRALGGGCRLPIAAYGFMEGKRLTLEGLLAAPNGREVLRDKVWGE
                     PDEAEAMGQRLAEMILEKGGRRLLELMG"
     gene            complement(15526..16788)
                     /gene="hemA"
                     /locus_tag="PLA58_02945"
     CDS             complement(15526..16788)
                     /gene="hemA"
                     /locus_tag="PLA58_02945"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOR61784.1"
                     /translation="MIGLVGLNHKTAPLAVRERLFAGCQEQANLLADLKAIDGVLEIL
                     HLSTCNRVELVASLEDAPRALGELESFLARYGGLADEEAAACLYTYRDREAIRHLFRV
                     TSSLDSLVMGEAQILGQVKDAYRLALAQNATGVVLNRLMHRAFRAAKRVRTETGIAAN
                     PVSVSFAAVELAKKIFGTLAGRKILIIGAGEMAELTCTHLVGNGAEDITVVNRSPAQA
                     RLLAEKFHGAAVGLEALDEALCDVDIVISSTGAPTYVVTGEMVRRCLRKRKNRLLFLV
                     DIAVPRDIDPAADEVENVYLYNIDHLQDIVDENIKNRRREALKAEAIIEEEVANYTNW
                     LKELEAVPTIVSLRSKAEGIVRTEMEKASGWMENLENADREKVEALVHGIVNKVLHAP
                     VAVMKEESAEFASQDIVAAARQLFRLDD"
     gene            complement(16797..17612)
                     /gene="ccsA"
                     /locus_tag="PLA58_02950"
     CDS             complement(16797..17612)
                     /gene="ccsA"
                     /locus_tag="PLA58_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOR61785.1"
                     /translation="MEGILFAAALFCCSLATGGYLISLLVRRVALAKISTWILAAGFM
                     LLALNLLFAAVHWTDWSSFGSRHFLSIYAWVIAGIYLGLQLKTKTRLLGAFIAPFILL
                     FLIAAAGQGSDRNLVPSQWQGGLTTLHLVLAIAGEALFVLASAAGAMFLIQNNLLKNK
                     KPGPLGRLLPSLGDLDQINHFGLLWGFPLLTLGMIGGAVYAAFVWGGSWPADPKVVWS
                     FAVWVFYGFLLHQRLAIGWKGSRMAVLSCLGLIAFLLSALGIRFCLATVHNFI"
     gene            complement(17614..18249)
                     /locus_tag="PLA58_02955"
     CDS             complement(17614..18249)
                     /locus_tag="PLA58_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOR61786.1"
                     /translation="MKYYPVFWDITGKKCVVIGGGEVAARKVARLLDCGAKVHVVSPL
                     LVPELADLKKRGRIGHIAGEYADEHLSGAALVIGATDDEEINASISRDAKARGIPVNI
                     VDDPQKCDFILPSLVERGDLTIACGTGGNSPALARRLREELEAAYGEEYAALLDILGQ
                     LRSKMEKNAGRGRVWFDQLMAAGLLESLRQKDADAAKKIVREITGEEVRID"
     gene            18459..19658
                     /locus_tag="PLA58_02960"
     CDS             18459..19658
                     /locus_tag="PLA58_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOR61787.1"
                     /translation="MLTEKNLQHYADVLLWALATAKKSALKKNDIILIQYDRPALRLA
                     EILYDRLMDKGVHPVQRMAATFRMEKSFYHKAGPQQLTFLAPGDKELYRHIHGRIFLH
                     APESLTHLRDVDPAKIGKAMVARKPLKNILDGREELRKYSWTLCLLPTEELARQANMP
                     LAQYARQVIRACYLDKKDPVSEWKAIHREVDHIKKALSSLAVKSFHLESDSMDLRITP
                     GEQRKWAGVSGHNVPSFEVFVSPDWRGTEGVYYANLPSFRSGNYVKDVRISFARGKAV
                     AVEAAQGEAFVKKQLAMDPGACRLGEFSLTDRRFSRIDRFMAETLFDENFGGKEGNCH
                     VALGSSYSDTFSGNPSRLTPALRKNLGFNDSALHWDLVNTEPKTVTAHMTNGRKTVIY
                     EGGEFKI"
     gene            19673..20767
                     /locus_tag="PLA58_02965"
     CDS             19673..20767
                     /locus_tag="PLA58_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOR61788.1"
                     /translation="MQEELLIQDARKSLRIRPEAAALYVKVKDSLLDHVNASMTEHPR
                     IADLIGGNPMSMMRDNHSNHLDFMSTVFEFNSFELLVKTVPWVYRSYHAHGFSFDYFP
                     RELEAWKNAVKTFLTPEASKEINAVYDWMLKNHGRMIELSAILPDKREPHPELKEERR
                     KFGACLLAADFPLGMKIAGSVLERENGQEALYLGLIQPVMYEIGRLWEQDKISAAEEH
                     MATSMVGRILAGLYARLPVSSANRGRAVVTSAPNEFHELGARILADMLEADGWDIFFL
                     GANTPVEELIKLVRKADPRFLAISVTMPFNMDRVATIISGIRNDSALSSVKILVGGAA
                     FNADRTLWQKIGADAWAEDPPSAIRQVQSW"
     gene            20761..21723
                     /locus_tag="PLA58_02970"
     CDS             20761..21723
                     /locus_tag="PLA58_02970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOR61789.1"
                     /translation="MVKGRGAPTDLESLLRENPEEITGYLLSQGRMAMILLDQNGVIL
                     DCNAFFLESVGLSDKPVGKSIQAFLREGLQAGWDCVRADCRPVKLTFTLDGPLEYSLS
                     GHVLNVHEHYLIFAHTVRLTDHEMVATLSRLTDELTDLTRQLSKKNRELEAANETITR
                     LMNTDPLTGLTNRRQLKERMDVELSKARRHGRPLSALMTDIDHFKFINDTFGHGAGDA
                     VLIGVASILKQTCRKEDIVARYGGEEFIVLLPDSPAAAALDCAERIRKAIEENVFPEI
                     DRKVTVSLGATLFGPTDTEDSFIGRADAALYEAKASGRNRVVLK"
     gene            21911..22999
                     /locus_tag="PLA58_02975"
     CDS             21911..22999
                     /locus_tag="PLA58_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOR61790.1"
                     /translation="MEKQIQAAAGHASGVFADCELGSGFDEEFFLLNFGTRLLTAFTD
                     REILIDIALETLADFSRGKRIAIMSLDESGKTLKVDGAFHQNQSVRPKVSFPVEGTVS
                     GKWVSQKTVGVAPLALEGEVPLPAKGERFGETQCLCLPLVGSSLRAVGLATIEIPADR
                     CYDFFEAQQLRVLSTALAVSLENAKLFSRVIHDSLTNLYTRRFYEIRVEEELAKLKRN
                     SGHLSVILFDLDEFKKVNDSFGHLTGDEVLRQFSALMMNHVRKGSTLVSRYGGEEFIL
                     LMPDARVEEALHLAERITSLCAEHDFGDDLRKIRMTVSGGVAFTDYTEAVLPAELFER
                     ADQALYEAKHRGRNRVVVWKNKSAPHRI"
     gene            23204..24562
                     /locus_tag="PLA58_02980"
     CDS             23204..24562
                     /locus_tag="PLA58_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61791.1"
                     /translation="MNQTIRKYLSNLENLFSFSPADAQGAAARFHEEMKRGLNGQTSS
                     LRMLPSYVGRPTGKEQGRFLALDLGGTNLRVLAVRLDGRGKAEISAVNRLAVPETVMR
                     GPGEELFDFLAEGVAVFLEEHRLDPKEHRDLAFTFSFPVAQSAVNAGVLLNWTKGFTA
                     PGVAGQNVVALLSDALRRKRIEAVSVTALVNDTVGTLATKSYADPSCDMGVILGTGTN
                     ACYPEKNSRPAKGLSPQPRQEMILNLEWGNFDKLDTNACDQALDRITLNPGRQRMEKM
                     VSGMYIGELVRLILLDMTDRGILLEKKHRSVLVREHAVSSEQMSLTVPGEDFFAAIGI
                     PDASDQDRDAAHHVCRIVSRRSARIAGAALAAVVTWMDANLEKKHTIAIDGALFEKYP
                     GYPENVRCVLEELFARRAERIALCTTSDGSGIGAAIIAAVASSRQEQEHKPARQRTRK
                     NR"
     gene            24686..25522
                     /locus_tag="PLA58_02985"
     CDS             24686..25522
                     /locus_tag="PLA58_02985"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOR61792.1"
                     /translation="MKTQSGNFKAHDGTSIYWKGWTPDNAPKAVVHVIHGYAEHIDRY
                     GNVVGELLPAGYAVFGTDHRGHGKSEGKRGHVMSFQEFIDDEKQFQREVIRTKFPKLP
                     CFVLGHSMGSLIAMNLVEQSAEGIRGLVLSGTGSRPGTNIPKILLAATRILSRLLPSI
                     HVKSPLPPDFISRDPEVVKAYVEDPLVYNVITPRLAYEMNRYVVIGAENSGEIQIPVL
                     IQLGSRDTAFSGQKELFEMVGAKDKTFKLYEGLKHEVYNELAQDRIKVLQDLRLWLDA
                     HV"
     gene            complement(25612..26019)
                     /locus_tag="PLA58_02990"
     CDS             complement(25612..26019)
                     /locus_tag="PLA58_02990"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HOR61793.1"
                     /translation="MPDFTRYSLALLEGGQMLYCAGGGGLRPLWDALEKFQGRSGLIL
                     HDKVIGLAAAMLIVRSGIVVEIHTKVASRPAVDFLEKNGIILHAAEVAANILTRDQSA
                     VCPGEIIALSCSNTDDFMKSIRAFLGSQAKGSH"
     gene            complement(26019..26513)
                     /locus_tag="PLA58_02995"
     CDS             complement(26019..26513)
                     /locus_tag="PLA58_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61794.1"
                     /translation="MMNSSVTINGIRSFRRIALAFGLLVLLPCVLSLINITFAHHWKI
                     HFFPAAIFLAAVCFGPAGGLAAGITGSLYSAILLGNPYLLIGNALLGLMTGVFFRKTG
                     KVIPSVGLAFACQLPWLVVTDFYFVGLPAVYIAKLTIVLFLGNLLWALLIHPAVGPVK
                     KYLC"
     gene            26715..27068
                     /locus_tag="PLA58_03000"
     CDS             26715..27068
                     /locus_tag="PLA58_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOR61795.1"
                     /translation="MPRPKCHRQICGIPDKNYFKPRGIPTADLEEVVLSLDEFEAVRL
                     ADYEQLYQEEAATRMNISRQTFGRIVASARKKIADVLINAKALKIEGGKVTLNEIAFC
                     RRGKGSRLRKNHPSL"
     gene            27081..27443
                     /locus_tag="PLA58_03005"
     CDS             27081..27443
                     /locus_tag="PLA58_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOR61796.1"
                     /translation="MKIALPSYQNQVDEHFGHCEYFTVFTTDDNKNILSEEIITPPPG
                     CGCKSDISLILAQMGVKYMLAGNMGQGAVNVLGANGIEVLRGCSGNVREVVQKWLAGS
                     LADSGLSCSQHEHTCHEH"
     gene            27555..>27906
                     /locus_tag="PLA58_03010"
     CDS             27555..>27906
                     /locus_tag="PLA58_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOR61797.1"
                     /translation="MESKKTCDSCSDAGCPAKQQEQNENQEAYADRRRLQARLCRIRH
                     KIVVLSGKGGVGKSTVAVNLATALALHGLRVGLLDMDIHGPSVPTMLGLEGESLKGNP
                     GELFPVEFGGLKVMS"
BASE COUNT         6096 a         8243 c         7479 g         6088 t
ORIGIN      
        1 tcttgtgaac gggcagttcg gaaaggatat gctcccggaa cagaccggcc caggatccgt
       61 ctaaaagttt gcgtcttttg ggtcccaggt agtcccaggg gtcgattaag tagggcgttt
      121 tgtgatcacg ggcatgaatc atggcgttaa cacctatctc cttaatattt aacatgattc
      181 atgccatttc agagaaaaac cgtcaaggaa aaaatgcata aatgctaaat tattttaatc
      241 tctcagggtt gaattccccg ccgcttgcgg cgaatggccc atacaaaata gaaagctttt
      301 gataccccgc tgcttgcggc ggggttgttc attcgagatt gtggttaggt cctgaattat
      361 cagtttcagg caagatgctg ttaatatttg acagcaccgc tgactttttt ctcatctaag
      421 acaaagccgt aattattttt tccgccagtt cccggccgat tcccggcacc ttctgcaaat
      481 cctccaggaa ggctttgcgc acctgttatg ccgtaatttc ttctattggc aatatctgct
      541 tgccatgccg gattgtgaga attgatactt gtttcttttc gatgcgatat acgatgcgat
      601 aattgccgta gatcagctct ctaaattgtt catcttcaat ttcaggaaca accctgccga
      661 tttcgggcga agatgcgagt tgctctactt ttgaaaatat ggtggtaatc catttgtccg
      721 ctgctgccgg tttgtctaag gctatatatt cagcgatttc cgaagctctg tcgatagcca
      781 acggcgacca aataattttc atttatggac tcttcttaag actttatttt ttgcatcttc
      841 atgcgtaatc ccttgcccgt ttgcaagttg accaagagat gtctgaacat ccgttagaag
      901 ctcaagcctc tcctgcatgt tttcatattc agaggcatcc aagagaacgg caactccttt
      961 cccatgttgc gtgatgatca ccggtctttt tgtatcacga acttgcttga tataggttgc
     1021 gatgcctatc cttacctctg ataggggttt aatatcttga tcaattttca atctttgcat
     1081 tttacacctc atcattaact aattttgtac cttatagcgt acaatattta gtattagcaa
     1141 gaagtttttt cataagaggt taatgggggc ctgaattatc aatttcaggc aagatgctgt
     1201 tactatttga cagcaccgct gaccttttct catctaagac aaagccgaat aaattttttc
     1261 cgccagttcc cggccgatgc ccggcacctt ctgcaaatcc tccagtgagg ctttgcgcac
     1321 ctgctgggcc gaaccgaaat acttcaacag cagcgtcctt cgcgacgggc cgatatcggg
     1381 aatcgcatcc agaacggagc tcaaatcatt cttcgttttg accttgcgat gatagctgac
     1441 ggcaaagcgg tgggcctcgt cccgcagccg ctgcagaagg gccagcgcgg cgggcgagga
     1501 agaaagataa attgcgtctt tgcgccgggg cacatagacg cgatcctcgg attttgccgg
     1561 ctttccctga agtcttcttt tggccgccag aaaagacctt tcttctttgg ccaggccgat
     1621 gacgtccagt ttgatcttca agtcgctcag aacggcaaga gcgacgttga gctgcccctt
     1681 gccgccgtca accaccagca ggtccggcgc cgggtccgga ccctggaaac ggcgcgtcag
     1741 cacctcgcgc atcattccat aatcatccgg ctcgtcagga ccgcggacgc gaaaacgccg
     1801 atagcctgat ttttccggtt ccccgtcctg aaacaccacc attgacccca cggcgctttt
     1861 tccgccgaga ttggagatgt cgaaacattc gatccggcgc ggcaaagcgg caagcatgag
     1921 cttttcctga aggaacagca aagcggcctc tttctgctct tccttcctgc ggtcggacgc
     1981 ctgaagttcg cgggcattgg cgcacgccag atcgaccaat gcttttttcg cgccccgccg
     2041 cggcacggta atgcgcactc ttttttgctg tttttcggcc agccattcga gcaaaacggc
     2101 ttcatcgggg agactgtacg gaaggaggat ctcttccggc aggccggctt catcataatg
     2161 ctgccgcagc gccgatgaga gaatttcttc ccggtccgct tttgttttca ccggcgtaaa
     2221 ggatttgctg cccgtgagct tgcctgcgcg cacaaacaga atgcagatcc ggtgcgtttc
     2281 ggaatcggaa aaaacgccca gcacatcctg gtctgtctct gacccgccgt ccacatgctg
     2341 ctgctccagc gcgtgtgaaa gcgcgccgat gcggttgcgc agccgcgccg cttcttcaaa
     2401 gttcagctcc tgcgccgctt gttccatctg ctttttcaaa tcggaaatca gttcacggcg
     2461 tcggccccgc agaaaggaca cggcattttc caccagcgtc cggtatgccg cttcatcaat
     2521 ccggttttta cagggcgcca gacaccgtcc gatttgatat tccaggcacg gcctggcccg
     2581 aagctgaaaa tcccggtccc ggcatgtgcg caggggaaag atgcgttgaa tgaaacggag
     2641 cgtttccttg gcggccagcc ccgacgggta cggaccgaaa taaagcgccg cgtcattttg
     2701 tcttttgcgc accagccgca ggcgcggaaa cgtctccgcg ggatccagcg acagatggta
     2761 ataggttttg tcgtcccgca gaatgacgtt gtagcgcggg cgatgttttt tgataaggtt
     2821 gttttccaga atcagcgctt ctttttccgt ggcggtcgtg atgaacgcaa tatcgcttgc
     2881 cctcgccatc agaaacggcg ccattggcct agtgtccctg ccggtcaaat aggaactgat
     2941 gcggtttttc agatcattgg ccttgcccac ataaatcacc ttccccgacg cgtcctgcat
     3001 catataaacg ccggggcatc gcggagcgga agtcatcttt tcttttaagg cggtattctc
     3061 gtccatcaat acttccaacc attactcatg gttcgacagg ctcaccactc gacaggctca
     3121 ccactcgaca ggctcaccat gacacaactt tttgtcaccc tgagccggcc gaagggcgac
     3181 agggcctgcc ctgcgtttat cgaagggccc accaagacaa ttgccctatc agcggggcta
     3241 aagccccgca ctacactgcc catcgcccat gccccgtagg gaacggttta aaaccgttcc
     3301 ctacatttct tttccctatt gcccatcaat cattacgcat gaccctttat attcttccca
     3361 tacgcttctc aggacgtccg gccggtttgt aaaaatcccg tcaatcccga ggtccagcaa
     3421 ccgccgcata tttttttcgt cattgactgt gtagatatgc acgggaatgc cgtgaagttt
     3481 caaatcggga agccactttt cgccgagctc gcggagcgaa cagttgaagg cgtcggcgcc
     3541 aaggcattga acaatctccg agggcaggcg caagccgtgc tgcttctttt catagagcac
     3601 ggccacgggc gtcagcgcat cgatctgttt gaaatgcaaa atggcgcggg aatcaaagct
     3661 ggaatacaca acgtgctccc gcatgccggt ttcatcgaca attttgaggc atcgctcctc
     3721 gatcccgccg gaaacggcat ccgttaccgc ctccgttttc acctcgatgt tgacggcaat
     3781 tctgtccttg cagagcgcca gaacttccgt aagcgccgga atgcgctccc ccgtaaattt
     3841 tttgtgaaac cagccgccgg catcgagttt tcgcaaccgg atgaacgggt aatcggcgat
     3901 ccttccccgg ccgtccgtgc agcgcgagat gttttcatca tgaaagacca ccacttcccc
     3961 atccgccgta agctggacat ccagctccac catatcggcc ctcatgtcga tcgctgccct
     4021 gaaggcgggc atggtatttt ccggataata ggccgacgcc ccgcgatggg caataacggc
     4081 aaacgcggct cttcccctcg cttcccggga tgcccggttg ctttgccggt cttcaggata
     4141 gatcaatgca tgatagggta tggccatagg cagccccgct ttgcttttcg gtgtccggtt
     4201 ttttaacata tttgcctgca gagttcttca actattttat cgtttacaaa aaacggggaa
     4261 aaaggtacgt accgcatcag gatatcgata ggttttcatt tctgacggac agggaggaaa
     4321 atatgaaagt acttggcatc atgggaagtc cacgtgtcgg agggaacagc gatgttctcc
     4381 tcggccaggc cctggaaggc gccaaagcag ccggagccga tgtgaaaaaa atcatcctgg
     4441 cccggaaaga gattgccgga tgcagagact gcaagaagtg caacaaaacg ggaatctgcg
     4501 ccctgaagga tgatatggcc gaaattcagg agcaaattct ggaagccggc gccgtcatcc
     4561 acagcgttcc cgtttatttc tggacgatga cctcgcagat gaaagcctac ctggaacgct
     4621 ggtgcgtctt tttcgacgcg gaatggaaat ggcagaaagc ctactatccc aaaatgaagg
     4681 gcaagcgcat cggcctcatc acggtctgcg gagatccgga tccgcacaca gccgacccca
     4741 tcgtccacag tttccgaatg accgcggaaa tgaccagact gaactggctg ggctgcgtga
     4801 tggcttccgc taccgacaag ggggaaatcg ccggcaacga ggccgccctc tcggaggcgt
     4861 ttgatctggg gaaaaaagcc gcagcgccgg tgtcatgatt ttttaaatcc ggaagattgc
     4921 gcgccggctg cgaagagctt ccacggcggg tcaaaagaat ttggcgacca gatcggtgga
     4981 agattgacgt ccgattttgt cgaaatggtt tgtgaggaat tccccggcct tttcccggcc
     5041 gatattaaac aggtattgca gaaattccat atccagattc attttgctcg acgcggagag
     5101 cttcgccatg acttcctcgg cgtcgatggt gtggataaac gtgtgcttgt gcttggacga
     5161 atccagctcg cctttttcga ttaaagacgt aataaaataa atagcccgca tttcccgcat
     5221 caggctggaa ttgaagctga gcgtgttgat ccggtcgaag atctcgttag tggtgcgggg
     5281 aacttcgggg atgttgatcg ggttgatctg gacgatcagg acgtcgcggc agtcggtgtc
     5341 atagatcacg gggaaaatcg gcggattccc catatagccg ccgtcccagt agtactggcc
     5401 ttcgatttca ggcgcctgcg tcaggtaagg caggcaggcg gaggccataa tcatgtccgg
     5461 cgtgatttcg cgattttcaa aaaccttcag gcggccggtc agcacattgg tggcgcagat
     5521 aaaaagcctg atgctgcgcg acttctgcag cacggaaaaa tcgatcagct cttcgagcat
     5581 ctctttgagc gcgttcttgt tgttggggtt ccactggtag ggggaaaaca tgcgcgaaag
     5641 aacatcgaac atctgccagt atggagaaaa ttcaatcgac cctcgactgc ccaggagctt
     5701 gtcgaagacg gtcggctgaa acggcgagca ctggctgttt ttcgaatttg tgatccagaa
     5761 atggttgagc agctcctgcg cttttttgcg gccgccggac gccaggccat aggccaaaat
     5821 ggtcgcgttt acggcgcctg cgcttgttcc gacaatccct tcaatatcca gacgctcttc
     5881 atccagcaat cggtccagaa ccccccaggt aaaggcgccg tgggcgccgc cgccctgcag
     5941 ggctaaattc agttttttct ttttggagcg aaccggcatg cccgctggcg atttcttcat
     6001 agagttttac ctttgatgga tgctcctgtg gaagagacac tgtgcaaaaa accaaccccc
     6061 gcttccgttg caacagacgg agagctttga aaaatgtcat ttcgaggagc gtgagcgacg
     6121 agaaatctta aagatggccg tttgttcaag atttctccct tcggtcgaaa tgacaaaaat
     6181 ggccgttatt caaagctctc agacgatgtt ttcccgcctc gacaacgaag attcccgagc
     6241 caggggatgc atcgtttatc tcaactgctg aaaaaccgct gtttctatag catatttacc
     6301 aaaagcatat caataataat tgcggctgga atagccgcct ttttcgccgg gagcaatcaa
     6361 cggcaaaggc cggtctttgc gtcttttcct ttcgatccca agatcttcag ccttttttca
     6421 atgcgcctcg tcccagttca cccccgaagc gatctccact ttcaggggca ctttcaggcg
     6481 aatcacgttt tccatttcct cccggacaag ctttttgacg tcctcttgtt cctgaaccgg
     6541 agcttccagg accagttcgt cgtgcacctg catgatcagg cgggcgtcaa ggtttttctc
     6601 cttcagcaaa cggtcaatgt tcaccatggc caccttgatc aaatccgcgg cagacccctg
     6661 gatgggcgcg ttaatggcca tccgttcggc aaactggcga acggacggaa tcttgctttg
     6721 cagatcgggc agccagcggc gacgattcag aatcgtgcag acaaagccgt cgcgccttgc
     6781 cccttccagg acaccgtcca gataggcgcg cacttttttg taacgggcaa agtagccgtc
     6841 aatatagact tgagcggttt tctgggaaac gcccagctcc cgggccaggc cgaacgcgct
     6901 cataccgtag agaatgccga agttgatgac tttggcctgc ctgcgcatat cggggctgac
     6961 catttcggga aaaacaccaa acacgtcgga agccgtccgg gtgtgaatat cggcatcggc
     7021 cgcgaaagcg tccagcaaag cctcgtcttc ggacaggtgc gccagcacgc gaagttcgat
     7081 ctgcgagtag tcggcggaaa tcaaaacaca gccgggggga gcgataaagg cctgacggat
     7141 tcttttgccc tcgacggtcc ggacgggaat gttctgaagg ttggggttgc tgctcgaaag
     7201 ccggccggtc gccgccacgg tctggttgta tgacgtatgg atccttccgg tttgcggaga
     7261 aatcagcgcc ggaagcgcgt ccacatacgt ggatttgagc ttggctaaag accggtaggc
     7321 cagaatttcg gcgggcagct cgtggctccg ggccaggtcg gagaggacat ccacatccgt
     7381 cgaataacct tcttttgtct ttttgccctt cggaagctgc atcttttcaa aaagaatcat
     7441 ctgcaattgt ttgggggaat tgatattgaa tgtttccccg gccagacggt atattttctc
     7501 ctccgagagg gccagcagtt gcgacagctc tccggacatt tgctttaaaa gcgcggcatc
     7561 caccagaacg cccttttttt ccatcccggc cagcgtaaac agaagcggca tctccacgtc
     7621 tcgatacagt gaggaagccc cggcctgatc cagcccggcc agcagttttt ccgccagtga
     7681 aagcagggcc gcgacacgct gaccggcgga tagggccgcc tggtcaacag gcgcaggcgc
     7741 aagcgactgg tttttccccc tgccacccgc cgcttccgcc atggaaggga cgcgctcctg
     7801 caggtattcc cacgccactt cgtcgagcgt ataggaatgc cgcgcggggt tgagcagata
     7861 agcggccaat tgcaaatcca tacccgccga aaccggcagg tccggcagca ggaccagcgc
     7921 tgttttcaaa tcgtatgcgt atttggtgat tttcggattg gcccagaaag gcataagcgc
     7981 gggccagacc tcttccgtct tgagctgctg tccggcgccg tcatgcccca gcggaatata
     8041 aaatctttcc cgaccggccc cgatcgccat tccgatcagc gcctgcggcg tatttttttc
     8101 cgggaacatt tcacacgcga aggttctcac gcctcgaagt gctccgacca gctccgcaag
     8161 ggccttcgtg tccgccacga ttttgcaggg aacctccggt tttgccgggc cggctgaaat
     8221 ttgctgcaaa agcgatgaaa attcaaaatc cctgaagagc cgggaaagcg cctccctgtc
     8281 gggttcccgg cgcgccgcat ccttcagatc aaaatccaaa tccacgtccg tggaaatcac
     8341 cgccaaccgg cggctgagcc gggcctgatc ggcgtatttg cggaagcttt cacgcagctt
     8401 gacgttgtgg agcttttccg cgttttccag aaccgcttcc acggatccgt attcttcaat
     8461 cagcctccgg gccgttttgg ggccgatgcc ggggacgccg ggaatgttgt cggatgcgtc
     8521 gcccatcaac cccaggatct ccaccacttt gtccggaccc acgccgaatt tttccctgac
     8581 cgccgcgctg tcataggttt tgtccttcat cgtatcgacc agggtcaccc cgtcgccgat
     8641 gagctgcatc aaatccttgt cgccggagat tatcacggcg cgccatccct ttttccgcgc
     8701 ccggaccacg agcgtaccga tcagatcgtc tgcctccgtt ccctgcttct ccagaatcgg
     8761 gatggaaaat ccgcggatca cttccttgat gaggggaacc tgcgcgatca gatcctccgg
     8821 catgggcttg cgtgtcgctt tgtaatcctc atacgcttca tgccgggttg tcggcccctt
     8881 gagatcaaag gtcacaacga tataatccgg atggatatcc ttgagcagct tcatcagcat
     8941 aatagtgaaa ccgtaaatcg cgttggtggg gaagcccctg gaattggaca gcgcgggaat
     9001 gccgtagaaa gcgcggtaca aataattgct gccgtccaca acggccagga gggaatttgg
     9061 attcttttca gtcatcgttc attgtctcta caggtcaaag gacgcgtcga tcttgaaggt
     9121 cctgaccgcg cgcaggtcca gaatgtccgt aacaccggtc ttttttttca catccgacag
     9181 aagggcttcc agttcttcgg cactcgccgc ggtcgccgtg aaccagatat tgtattcgtg
     9241 cgcgcggcgg taattatgcg tcacgccttt ctcggcgctg acgatccgaa caaaggaccg
     9301 gatttggcct tcgggcacac gcgccgcaca cagcgtgctc gcgcgtccga gtttttttcc
     9361 gtcgaacacg gcgccgatcc ggcggataat gccccggtcc ttcagctttt gaacgcgggc
     9421 cagcgtttcc tcttcactga ttccgcagcg ctcgccgatc aaacggaaag gctcctcttc
     9481 cagtggaaat tccttttgca gaagattaag cagtttttta tcaatggcgt ccatacggcc
     9541 tcttcctccc ccggcaggag ggaattaagg ggatggaaac tatccggaga acgctttcgt
     9601 gtgccttcat tcattgccgc ggttgataca aacacaaagg ctcctccgcc cgataatcac
     9661 ccgtcgcttc ataggcccgt gcccggcagc cgccgcaaac tttgataaac tcgcagcgcc
     9721 cgcacttgcc cttgtagctt tcgtaatccc gaagattgcg gaacacttcg gaggtttccc
     9781 agatttcgcc gaagctttgc ttcctaatat tgccgcagtc aagctccaga tagccgcagg
     9841 gctgcacctg accgacatgg gatataaaac aaaaggagat gccgcccagg cagccccgtg
     9901 tcgattcatg aaaatgaccg ccgacttttt ttgcggggtt ttcccctttc cgtttattct
     9961 ggtgcataac ccggaaatag tgcggtgcgc aggtcgcttt gagctggatt gcgcacgtca
    10021 gactttcccg gtaaaaccag cggagcgtct cttcgtaatc cgccgcggtg atcgcctgct
    10081 cggccaaatc ccttcccctt ccggtcggca ccagcaaaaa gatgtggtgc gccgccgctc
    10141 cgatgcgaag cgccagatcg tgaatggctt tgatctgatg aagattggcg gtcgtgatcg
    10201 tcgtgttgat ctgaaattcc attccggcgg ctttcatcgc cgcgatgccg gcaagcgcgc
    10261 cggcaaacgc gcccggctgc tggcgcaacg cgtcgtggct ttgcgcgtcg ggcccgtcga
    10321 tgctgatgct cacccgctga atgccggaat cgatcatctt tcgaaccttg gcttcatcga
    10381 ccagcgtacc gttggtcgcc atgaccatgc gcaggctttt ttgtgtgccg taggcggcca
    10441 gatcaaagat atcctgcctc aatagcggtt cgccgccggt gagaatgatg accggctgcg
    10501 aaaaacgggc gatgtcgtcg atcagacgga gacattcgcc ggtggaaagc tccccaggat
    10561 aaggcccgag atgcgaggcg gcacggcagt gcacgcagtt cagattgcag cttcgcgtca
    10621 cttcccacgc aatcatccta acctgatccc gcctttcctt gcgggatgtg tgcccttcag
    10681 ggtaaggacg ggatgaggcg cccacatcca cgaccgttgc acgatcggaa tccctcctcc
    10741 ccttgtggga ggggcagggg gagggggaat ctccactcat gtcttttcgc tttccgattt
    10801 tcacgtcgcg agcaccttcg ccagatcttt tgcccagtag gtaatcagaa tgtccgcgcc
    10861 ggcgcgcttg atggccgtga cgctttccag catggctttt tcttcatcga gccagcccag
    10921 cttcgccgcg gccttgatca ttgaatattc cccgctcaca ttataggcgg caagcggcag
    10981 atcgaattcc tgcctggccc ggcaaatgat gtccagatac ggcagcgccg gcttcaccat
    11041 gatgatgtcc gcgccttcat tgacgtcgag cgaaatttcc cggatagcct cgtcggaatt
    11101 ggccgggtcc atctgatagg cctttcggtc gccgaactgc ggcgcgctct cagccgcctc
    11161 gcggaacggc ccgtaaaacg aggaggcgta tttcgcggaa taggccatga tcggaatgtt
    11221 ttccaacgcg ttttcatcga gaatctcgcg gatggccgcg atttgtccgt ccatcatggc
    11281 cgacggcgcc accatgtccg cgccggcttt cacctgcgac agggccgttt ccgccagcac
    11341 ctcgagcgtg gcgtcgttca aaacttcgcc cttttcgatg atgccgcagt ggccgtggct
    11401 cgtgtattcg cacaggcaga cgtccgtgat caccagcatg tccggcactt cgttcttgac
    11461 gcgcttcacg gcctgctgga cgatcccgtc tttggcaaac gcgccggttc cctgctcatc
    11521 cttcctggcc ggaatgccga aaagcagcag cgcggggatg cccagcgcat gcgcctcgcg
    11581 cgcttccttc gcaaggtggt ccaccgacat ctgaaactgc cccggcatcg aggcaatggg
    11641 ttttttcaca tccctgcccg gcacggcgaa aagcggataa acgagctcgt ccgcgctcag
    11701 gcgcgtttca cgaaccagtc tgcgaaagtt ttcattcttt ctcaaccggc gtccccggta
    11761 ttcaggaaat tgcatatttt actcctttgg tgatgatatt tcctcgtcgg tcagatagca
    11821 ggccggatcc ggcccccact cgtccccctc cgcttcggcc cgcgcccgga agttcccgcc
    11881 gcacacggaa agccaaacac atgccgcgca gcgtccggta acgtattttt tcttatcctt
    11941 cagtttcatg agaaactcgt ttttttcgtc cgtccagatt tcgctgaagg gccgcttccg
    12001 gacattgccc agcggccggt tgcgccagaa ctgatcggga tacacttcgc cgtcccagct
    12061 gatacagccg atgccgacgc cggtggaatt accctcgttc atttccagga gctccaggat
    12121 ttccgccgcg cgcgccgggt ccttctccct cattttcaga tacaggtaag gcccgtcggc
    12181 gtgattgtcc accgtgagga tttccggcgc caggccgtcg tcaaacatgg ccttcgtctt
    12241 atcggcgatg agatccagca ggcggcgggc ctccccgtgc gtcaggtcct cctcgcggag
    12301 cttcgagccg cggccggaat acaccagatg gtaaaagcac atccgctcga tcttttcttt
    12361 tctgagcagg tcgaacatgg ccggcacatc ggccacgttg tggcggttga cggtgaagcg
    12421 caggcccacc ttgattcccg cttcccggca gttgcggatg ccttccatgg cttttccaaa
    12481 cgagcctttt ctgccccgga accggtcgtg cgtcggctgc aaaccgtcga ggctgacgcc
    12541 gacgtacgaa aggccgattt ttttaagttc ggctgcgatt tctttggtaa tgagcgtgcc
    12601 gttggtggag atgaccgcgc gcatgcccat gtccgtcgcg tacctggcca gttcgagcaa
    12661 atccttccgc atcagcggtt cgccgccgga aaacagcagc accggcgatc cgaaagccgc
    12721 caggtcatcg atcatgccct tgccttcttc cgtcgtcagc tcatcgggat agtcgatgtc
    12781 agccgagctg gaatagcagt ggatgcattt gaggttgcag cggcgcgtca tgttccagac
    12841 caccaccggt ttcttgtccg ccgaaaattg cagcagatgc gacggcagcc ttcccgagtg
    12901 ccggttgtaa cgcagcacgt cggagggctc caccgctccg cagtagagtt tggagatccc
    12961 gatcatcctt ctttcctttc tgcccccccg cccgtcccgg gcgccgattt ctgaaaagcg
    13021 cggtaaagcg ccgcgaccag gccttccatg gtgtattctt cctgggcgat gtcgacggac
    13081 aggcccgcct gcttcgccgt ggccgccgtc acgggaccga tgcaggccac ccggacggaa
    13141 ggcggcagcg tgaaatcctt ccccataatg tcgaaaaagt tcgtcacggt ggaggagctg
    13201 gtaaacgtga tcacgtccac gtcgcccgcg gcaagccgcc gggccagctc ttcttttttc
    13261 ctgccggaat tcaccgtccg gtaagcggtt gccaccgtga cgcgcgcgcc ttgcgccttg
    13321 agcgtccggg gcaaaatatc gcgggctttt tccgcccgcg gaatgaggat gttcatcccc
    13381 tgaatatcca tggcggcaaa cgcttttaaa atgccttcgg caatgaactc ctcgggcacc
    13441 agatccgccc ggatgccacg gtccgccagt tgccgggccg tggccggacc gatgcagcag
    13501 attttgacgc ccttgagatc gcgcacgtct ttgccctttt gctgcatgcg ttcgaaaaag
    13561 aagcgcacac cgttggcgct cgtgaaaaca agccactggt aagcgtccag cccggccagc
    13621 gccgcgtcaa gttcactcca gtcggaaggc ggttcgatgg cgatggtcgg aaacgcgatc
    13681 ggccgcgcgc cttccgccgc gagaagcttc gccagatcgt cggcctgccg ctcgggccgc
    13741 gtgatcgcca cgcccagccc gaacaggggc ttcgtgtcaa accagccgag cgtgtcgcgc
    13801 agttcgacca cctttcccac caccagaatg gcgggcggtt taaagcggtt ttctgcggcc
    13861 agatcggcaa tcgcggccag cgtgcccgaa aggactttct gatcgggcgt tgtgccgcgc
    13921 cggatcaggg ccgcgggcgt ttccggcggc tttccctgcg caatcagcgc cccggtgatc
    13981 tgatcgagat ttttcacgcc catcagaaac accagcgttc caatgcccgc gagcgcatgc
    14041 cagtcgatgt cgcttttttc cttggtcgga tcttcatgtc cggtgatgaa cgccaccgtg
    14101 gacgtgaccc cccgatgcgt gagcggaatg cccgcgtagg ccggcaccgc gattgcggaa
    14161 gttacgccgg gaatcacttc gaagggaatg tgccgggcgg ccagttcttc cgcctcttcg
    14221 ccgccgcggc cgaagataaa gggatcgccg cctttgaggc gcgccaccgt atggccgcgc
    14281 agcgcttccc ggaccaggag ctcgttgatt ttatcctgcg tcagcgtgtg gtttcctccc
    14341 tctttgcccg cgtaaatgac gcgggcgccg gccggcgcat aggcgaggag gtcttcattg
    14401 accagattgt cgtaaaccac cacatcagcc agtcccaggc agcgggccgc cttgatcgtg
    14461 atgagccccg gatcccccgg ccccgcgccg atgatataga ctttcccagg tttggctttt
    14521 ttcattttac cccatcaact ccagcaggcg gcggccgcct ttttccagga tcatctccgc
    14581 aagtctctgt cccatggcct cggcctcatc cggttctccc cagaccttgt cgcgcagcac
    14641 ttcccttcca ttgggcgcgg ccagcaggcc ttccagcgtc aggcgcttgc cttccatgaa
    14701 gccgtatgcg gcaatcggca gccggcagcc gccgcccagc gcccgcagat aggcgcgctc
    14761 cgccgtcact tccgcttcag ttacggcatg gttgagcctc gcgcacagcc ccgccagctc
    14821 cgcatcgctt tttcttacct gaagccccag agcgccctgg cccaccgccg gcagcatgat
    14881 ttccgtcggc agaaactgtg taaccacttc cccgtagccc agacgcttca cgcccgccgc
    14941 ggcaagaacc acgccggtga gattctccgt ttcgatcttt ttcaggcgcg tgtcaatgtt
    15001 gccgcgcagc ggaacaatct ccagatccgg cataaacgat ttcaactgcg cgccgcgccg
    15061 ctgggagccg gtcccgattt tggccccctt tggcaaatat tcaaatttaa tcctgccgcg
    15121 ggtcaccaga acatcgcgga cgtcctcgcg cggcagaatc gccgcaaaga cgagcccctc
    15181 cggagtctct ccgggaacgt ctttcatgga atggacggcc aggtcaatct gaccggaaag
    15241 cagcgcctct tcaatttctt tgacaaaaac cccctgcccg ccgatctgcg tcagagacac
    15301 atcctgcata atatcgccgc tggtccggat aacgcagatt tccgcctcga tctccggcgc
    15361 cgctttcttc agaccggcca ccgcggaatt cgtctgcgcc agcgccagtt tgcttcctct
    15421 cgtgccaact ttaatcttca tcttttacct ttccatcctg ctcgcagcag ttgaatcggg
    15481 gcatccgctg agcgaaggga tgccccttca attgccgtat cttcttcaat catccagccg
    15541 gaagagctgg cgcgccgccg ctacgatatc ctgggaggcg aattcggcgc tttcttcctt
    15601 catgaccgca accggcgcat gcagcacctt gttgacgatg ccgtgcacca gggcttcgac
    15661 tttttcgcgg tctgcgtttt ccagattttc catccagccc gacgctttct ccatctccgt
    15721 ccgcacaatg ccttcggcct tgctccggag cgacacaatg gtgggcacgg cctccagctc
    15781 cttgagccag ttcgtgtaat tggccacttc ttcttcgata atcgcctcgg ccttcagcgc
    15841 ctcgcgacgc cggtttttga tgttttcgtc gacaatgtcc tgcaggtggt cgatgttgta
    15901 cagatagacg ttttcaactt cgtccgccgc cggatcaatg tccctcggca cggcaatgtc
    15961 caccagaaac agcagccggt tctttcgctt gcgcagacag cggcggacca tctccccggt
    16021 cacgacatac gtcggcgcgc cggtggagct gataacaata tccacatcgc acaacgcttc
    16081 gtcgagggct tcaaggccta cggccgcgcc gtgaaacttt tcggcaagca gccgggcctg
    16141 cgcgggcgac cggttgacaa cggttatatc ctccgcgccg tttcccacca ggtgtgtgca
    16201 ggtcaattcc gccatttcac cggcgccgat gatcagaatc ttcctgccgg cgagcgttcc
    16261 aaaaattttc ttggccagct ccaccgcggc aaagctcacc gacacggggt tggccgcaat
    16321 gcccgtttcc gtccgaacgc gtttggccgc ccgaaacgcg cggtgcatca ggcggttgag
    16381 cacaacgccg gtggcgtttt gcgccagcgc cagccggtag gcgtccttca cctgccccag
    16441 aatctgcgcc tcgcccatca ccagcgaatc caggctggat gtcacgcgaa acaaatgccg
    16501 gatcgcctcc cggtcccgat aggtgtacag acaggccgcg gcttcttcgt ccgcaaggcc
    16561 gccgtagcgc gccagaaaag attcgagctc acccagcgcc cgcggcgcgt cctccaggga
    16621 ggccaccagt tccacgcggt tgcaggtgga aagatgaaga atctccagca cgccgtcgat
    16681 cgccttgagg tccgcaagca gattcgcctg ctcctggcat ccggcaaaaa gcctttcgcg
    16741 cacggccagc ggcgccgttt tatgatttaa tccgaccagt ccgatcatgg cttctttcaa
    16801 ataaagttat gcacggttgc caggcaaaac cgtatcccta acgccgacaa caaaaaggcg
    16861 atcagtccca gacaggacag caccgccatc cgggaaccct tccatccgat cgccagccgc
    16921 tggtgcagca gaaacccgta aaacacccag acggcaaagg accagaccac cttgggatcg
    16981 gcgggccagg aaccgcccca gacaaaagcg gcataaaccg ctcccccgat catgccgagc
    17041 gtcagcagag gaaaccccca cagcaacccg aagtgattga tttggtcaag gtcgccaagc
    17101 gacggcagca ggcggcccag cggacccggc tttttgtttt tcagaagatt attctgaatc
    17161 aggaacatcg ccccggccgc ggaagccaga acaaacagcg cttcgccggc aatcgccagc
    17221 accaggtgca gcgtcgtgag cccgccctgc cactgggacg gcacgagatt cctgtcggat
    17281 ccctggcctg cggcggcaat caaaaacagc agaataaacg gcgcgataaa ggcgcccagc
    17341 agtcgggttt tggttttcaa ctgcaggccc agatagatgc cggcaatcac ccatgcataa
    17401 atcgacagaa aatggcgcga tccgaaactg ctccagtcgg tccagtgaac tgccgcgaaa
    17461 agaagattca gagccagcag cataaaacct gccgccagaa tccacgtgga tattttggca
    17521 agcgccactc ttcggaccag cagggaaatc aggtagcccc ccgtggccag cgagcagcaa
    17581 aacagagccg ccgcgaagag aatcccttcc acgctagtca atgcgcacct cctcgcccgt
    17641 aatttcgcgg acgatttttt ttgccgcgtc agcatctttt tgccggaggc tctccagcag
    17701 gcccgcagcc atcaactgat cgaaccaaac ccggcccctg ccggcgttct tttccatctt
    17761 gctccgcaac tgtccaagaa tatccagcag cgccgcgtat tcttcgccgt aggcggcctc
    17821 cagctcctcc cgcaaacgcc gcgccagcgc cggggaattt cctccggtgc cgcaggcgat
    17881 ggtcaaatcg ccccgctcca ccagggaggg caaaataaaa tcgcattttt gcgggtcatc
    17941 cacgatattg acgggaatgc ccctggcctt tgcgtcccgg gatatggagg cgttgatttc
    18001 ttcgtcatcc gttgccccga tcaccagagc ggctccggac agatgctcgt ccgcatattc
    18061 accggcgatg tgcccgatcc ggccccgttt ttttaaatcc gccagttcag ggacaagaag
    18121 ggggctcacc acatgcacct tcgccccgca atccagaaga cgcgcaactt ttctggccgc
    18181 aacttctccc ccgccgatga cgacgcattt cttccctgtg atgtcccaaa acaccggata
    18241 atatttcaag tttttccttt cagacccaaa caggaacggc tttgtgccat tggcggtcaa
    18301 gctatcatga agggttgcaa aattcaagcg ggggtttgcc cgggcggatt ttttatctat
    18361 ccggaatcag ttacttattg acactgccgc cgctttgcgt ctatggtttt gtgcaaatgc
    18421 ggcaaaggca aaaaaatatt tcaaaggaaa tcgtaaacat gcttactgag aaaaatcttc
    18481 aacattacgc cgatgtcctc ctctgggcgc ttgcaacggc aaaaaaatcc gccctgaaga
    18541 aaaatgacat cattttgatt cagtatgacc ggccggcgct tcggcttgcc gaaattctct
    18601 acgacagact gatggacaag ggcgttcacc ccgtccagcg aatggcggcc accttccgca
    18661 tggaaaagag tttttaccac aaagccggcc cgcagcaact gacttttctg gctcccggcg
    18721 acaaagagct ttatcgtcac atccacggcc ggatctttct ccatgcgccc gaatcgctga
    18781 cgcatcttcg ggacgtcgat ccggccaaaa tcggcaaagc gatggtcgcc cgaaaaccgc
    18841 tcaagaacat tctcgacggg cgggaggaac tgcgcaaata cagttggaca ctgtgcctgc
    18901 tgcccacgga ggaactggcg cgccaggcca acatgccgct tgcgcaatat gcccgccagg
    18961 ttatccgggc ctgctacctt gataaaaaag atcctgtttc cgaatggaaa gccatccacc
    19021 gggaagtcga ccacatcaag aaagcgctca gttcgcttgc cgtcaaatcc tttcacctgg
    19081 aatccgactc catggatctg cggatcacgc ctggtgaaca acgcaaatgg gcgggggttt
    19141 ccggccataa cgtgcccagc tttgaagtgt ttgtttctcc ggattggcgg ggaaccgagg
    19201 gcgtgtacta tgccaacctt ccctccttcc gcagcggaaa ctatgtaaag gatgtccgca
    19261 tttcgttcgc caggggaaag gccgtcgccg tcgaggcggc acagggcgag gcctttgtga
    19321 aaaaacagct cgccatggat cccggagcct gccggctcgg agagttctcc ctcacggaca
    19381 ggcgcttttc ccggattgac cgcttcatgg cggaaacgct tttcgatgaa aacttcggcg
    19441 ggaaagaagg caactgccat gtcgcgctgg ggtcctccta cagcgacaca ttcagcggca
    19501 acccctcgcg tctgaccccg gcgctcagga aaaatctggg attcaacgac tccgccctgc
    19561 actgggatct ggtcaacacg gagcccaaaa ccgttacggc gcatatgaca aacggaagaa
    19621 aaaccgtgat ctatgaaggc ggcgagttta aaatctaaaa tcgggagggc tcatgcaaga
    19681 agaactttta atccaggacg cgcggaagtc tttgcggatc cgcccggaag cggccgctct
    19741 ttacgtaaag gtgaaggact ccctgctcga tcacgtcaat gcttcaatga ccgagcaccc
    19801 ccgcatcgcc gacctgatcg gcggcaaccc catgagcatg atgcgggaca atcacagcaa
    19861 tcacctcgat ttcatgtcca ccgtctttga attcaacagc tttgagctgc tggtgaaaac
    19921 cgtcccctgg gtataccgct cctaccacgc gcacggcttc tcctttgact attttccccg
    19981 ggaactggaa gcctggaaaa acgcggttaa aacatttctc acccccgaag cgtcgaagga
    20041 aatcaacgcc gtttacgact ggatgctgaa aaaccatgga cgaatgattg aactttccgc
    20101 gatcctcccc gacaagcggg aaccccaccc ggaattgaag gaggaacgca gaaagtttgg
    20161 cgcctgcctt ctggcggcgg actttccgct gggcatgaaa atcgccggct ccgttctgga
    20221 gagggaaaac gggcaggagg cgctttacct ggggctgatc cagccggtca tgtatgaaat
    20281 cggcaggctc tgggaacagg acaagatcag cgctgccgaa gagcacatgg ccacttccat
    20341 ggtgggaaga atcctggccg gcctttacgc cagactgcct gtttcttccg caaaccgggg
    20401 cagagccgtg gtgacctccg cgcccaacga atttcacgag ctgggggcaa gaattctggc
    20461 cgatatgctg gaagcggacg gctgggacat tttcttcctg ggcgcgaaca cgccagtcga
    20521 agagctgatc aagctggtca gaaaagctga tccgcgtttt ctggccatct ccgtgaccat
    20581 gccgttcaac atggaccggg tcgcgaccat catttccggc atcaggaacg attccgccct
    20641 gagctccgtc aagattctgg tcggcggggc cgccttcaac gcggaccgca cgctctggca
    20701 gaaaatcggc gccgacgcct gggcggaaga tcccccgtcc gccatccggc aggtccagtc
    20761 atggtaaaag gccgaggcgc gccaaccgat ctggaaagtc ttctgcggga aaatccggag
    20821 gaaatcaccg gctacctgct gtctcaaggc aggatggcca tgatccttct ggaccaaaat
    20881 ggcgttatcc tggattgcaa cgccttcttc ctggagagcg tcggattatc ggacaaaccg
    20941 gtcggcaaat ccatccaggc ttttctccgg gaaggcctcc aggccgggtg ggattgtgtc
    21001 cgtgcagact gccggcccgt gaagctgaca tttacattgg atggcccgct ggaatattcc
    21061 ctgtcgggtc atgtcctaaa cgtccacgag cactatctca tttttgcgca taccgtccgt
    21121 ctgaccgatc atgaaatggt cgccacgctg tcccggctca cggatgaact gaccgatctg
    21181 acccggcagc tcagcaaaaa aaaccgggag ctggaagcgg ccaatgaaac catcacccgt
    21241 ctgatgaaca ccgatcccct gaccgggctc accaaccggc gtcagctcaa ggaaaggatg
    21301 gacgtggaac tgtccaaagc cagacgccac ggccgtccgc tgtccgcgct tatgacggac
    21361 atcgaccatt tcaaattcat caacgatacc ttcgggcacg gcgcaggcga cgcggtgctg
    21421 atcggagtag cctccattct gaaacagacg tgccggaaag aagacattgt cgcgcgctat
    21481 ggcggagaag aattcatcgt ccttcttccc gattcacctg ccgcggcagc cctggattgc
    21541 gcggagcgaa tccgaaaagc cattgaagaa aacgtgtttc cggaaatcga ccggaaggta
    21601 accgtcagtt tgggcgccac cctctttggg cccaccgaca ccgaggacag cttcatcgga
    21661 cgggcggatg ccgcccttta cgaggccaag gcatccggac gaaaccgggt ggttttgaaa
    21721 tagccggccc gaagcgccgc cggacggcag gaacgcgaat atcgtctgtg agcggaaaaa
    21781 aagattctct ttgacagagc ggacctgcct gtcctatact tcgggccgca ggaaaaagga
    21841 gccctgatga aatgcttttc tgtttccttt tcctgaacaa ccagatggca acgtgtatta
    21901 aaggaacgac atggaaaaac agattcaggc cgcagcggga cacgcctccg gcgtttttgc
    21961 cgactgcgag ctgggctccg gttttgacga ggaattcttt cttctcaatt tcggcacccg
    22021 gcttctcacc gcgtttaccg acagggaaat tctgatcgac atcgctctgg aaacgctggc
    22081 cgatttctca cggggaaaaa gaatcgccat catgagtctc gacgaatcag gcaagacgct
    22141 gaaagtagat ggagcttttc atcaaaacca atccgtccga ccgaaggtgt ctttccccgt
    22201 ggaaggaacg gtttccggga aatgggtgtc tcaaaaaacg gttggcgtcg ctccgcttgc
    22261 cctggaagga gaggtccccc tgcctgcaaa aggcgaacgc tttggagaaa cccaatgttt
    22321 atgcctgccg ctggtcggat cttccttgcg cgcagtggga ctggccacga tcgaaattcc
    22381 tgcggatcgt tgctacgatt ttttcgaggc gcagcaattg agagtcctca gcaccgccct
    22441 ggccgtttcc ctggaaaacg cgaagctgtt ttccagggtc attcatgaca gtcttaccaa
    22501 tctctacacc cgaaggttct atgaaattcg ggtggaagaa gagctcgcca agctcaagcg
    22561 aaacagcgga cacctgtccg tcattctttt cgatcttgac gagtttaaaa aagtcaacga
    22621 ttctttcggc cacctgacag gcgacgaagt tctgcgccaa ttctccgcgc tcatgatgaa
    22681 ccacgtgcgc aaaggatcca cgctggtcag ccgctacggc ggcgaagagt ttattctgct
    22741 catgccggat gcccgtgtgg aggaagcgct ccatctggcc gaaagaataa cctctctgtg
    22801 tgcggaacat gactttggag acgacctgag aaagatccgg atgacggtca gcggcggtgt
    22861 ggcgttcacc gactatacgg aagccgttct ccccgcggag ctcttcgaaa gggcggatca
    22921 ggcgctttat gaagccaaac acagaggtcg aaaccgcgtg gtcgtctgga aaaacaaaag
    22981 cgcaccgcac cggatataac tccggagcgc cgccgattcc gggagagctt tgcaaaagac
    23041 tacaaatcga gcacctttgt cataccggag caggccggta tccagtatga tcaagtattt
    23101 cctggattcc cgtcttcgcg ggaatgacgt aaaatgaagt tgtgcaggtc tctccggatt
    23161 cacgccctcg ccggaagcgc acggggcttt ttgtgtgccg gatatgaatc aaaccatccg
    23221 taaatatcta tcgaatctgg aaaatctttt ttccttttct cccgcggacg cacaaggcgc
    23281 cgccgcccgt ttccacgagg agatgaaacg cggcctgaac gggcaaacca gttccttaag
    23341 aatgcttccc tcctatgtcg gccgtcctac gggcaaggag cagggacgct ttctggcgct
    23401 cgatctgggc ggcaccaacc tgcgtgtcct ggcggtccgc ctggacggcc ggggcaaagc
    23461 ggaaatttca gccgtgaacc gtctggccgt cccggaaacc gtcatgcgcg gaccggggga
    23521 agagcttttc gattttctgg ccgagggcgt cgctgttttt ctggaagagc accggctcga
    23581 ccccaaagag caccgggatc tggccttcac cttttctttc cccgtcgcgc agagcgccgt
    23641 caatgccgga gttcttttga actggaccaa aggatttacc gccccgggcg tcgcgggaca
    23701 aaatgtcgtc gccctgcttt ccgacgcgct caggcgcaaa cgcatcgaag cggtttcggt
    23761 cacggccctg gttaacgaca cagtgggaac actggcgaca aaaagctacg ccgatccttc
    23821 ctgcgacatg ggcgtcatcc tcggcacagg cacaaacgcc tgctatccgg aaaaaaacag
    23881 ccgaccggca aagggtctgt ctccacaacc ccggcaggaa atgatcctca acctggaatg
    23941 gggcaatttc gacaagctcg acaccaacgc ctgcgatcag gcgctggatc gcatcaccct
    24001 caatcccggc cgccagcgga tggaaaagat ggtgtccgga atgtatatcg gcgaactggt
    24061 ccggctgatc ctgctggaca tgacggaccg gggaatactg cttgaaaaaa agcaccggtc
    24121 cgttctggtc cgcgaacacg ccgtcagttc ggagcagatg tctctgaccg tcccgggaga
    24181 agactttttc gcggccatcg gcatcccgga cgcctccgat caggaccggg acgcagctca
    24241 ccacgtctgc cgaatcgtct caagacggtc ggcccgtatt gccggagcgg ccctggcggc
    24301 cgtggtcacc tggatggacg caaatcttga aaaaaaacac accatcgcca tcgacggcgc
    24361 cctgttcgaa aaatatcccg gctatccgga aaacgtgcgg tgcgtcctgg aagagctctt
    24421 cgcccgccgc gccgagagga ttgctctttg tacaacgtcg gacggttcgg gaatcggcgc
    24481 ggccatcatc gccgccgtcg cgtcctccag acaggagcaa gagcataagc cggcccgtca
    24541 acgaacgcgc aagaaccgct gaaatcattt tccttgtttc cgttccgttc ctggtttata
    24601 ttcctgttgg aatttctccg aaaaaaagtt gcaaagcatc cggcgcctcc gggagccggc
    24661 ggtaacccaa cgaaaggaga tcctcatgaa aacgcaatcc ggaaatttca aggcgcacga
    24721 tgggacgtcc atctactgga agggatggac gccggacaat gcccccaaag cagtggtgca
    24781 cgtcattcac ggctacgccg aacacattga ccgttacgga aatgtggtgg gcgagcttct
    24841 gccggcaggc tatgccgttt tcggcaccga tcaccgcgga cacggcaaaa gcgagggaaa
    24901 gcgcgggcac gtcatgagct ttcaggagtt tattgacgat gaaaagcagt tccagcgtga
    24961 ggtcatccgg acaaaatttc ctaaactccc ctgctttgtc ctcggccatt ccatgggaag
    25021 cctcattgcc atgaacctgg tcgagcaaag cgcggaggga atccgggggc ttgtgttatc
    25081 gggcaccggc tcgcggccgg gaacgaacat cccgaagatc ctgcttgccg caaccagaat
    25141 cctttcccgt ctgctgccgt cgattcatgt aaaatcgccg cttccgccgg attttatctc
    25201 ccgggatccg gaggtggtca aggcctacgt ggaagatcct ctcgtgtaca acgtgatcac
    25261 cccaaggctg gcctatgaga tgaaccggta tgtcgttatc ggcgcggaaa attccggcga
    25321 aattcaaatc ccggttctta ttcaactggg ctcccgtgac accgcctttt ccggacagaa
    25381 ggaactcttt gaaatggttg gagcaaaaga caagacgttc aagctatacg aaggtcttaa
    25441 gcatgaggtt tacaacgagc tcgcccaaga ccggatcaaa gtgctccaag acctgcgatt
    25501 gtggctggac gctcatgtgt aagacaggaa cactgcccgg atggacgccc gtcggaaaac
    25561 cattgaacct catgaccgtc ccttttcaga ccgatgaaaa gagccgcatg gctaatggga
    25621 gcccttcgcc tgagaaccga gaaacgccct gatgctcttc ataaaatcat cggtattgga
    25681 gcaactcagc gcgatgattt caccgggaca gaccgcactt tgatcgcggg tgaggatgtt
    25741 ggccgccacc tcggcggcgt gaagaatgat cccgtttttt tccaggaagt ctacggccgg
    25801 ccgggatgcc acttttgtat ggatttcgac aacgatgccg gagcggacaa tcagcatcgc
    25861 cgctgccagt ccgattactt tatcgtgcag gatcaggccg gaccttccct gaaatttttc
    25921 cagggcatcc cacagcggac gcagaccgcc ccctcctgcg caatagagca tttgcccgcc
    25981 ttccagaagc gcgagggaat accgcgtaaa atcaggcatc agcataaata ttttttgacc
    26041 ggcccgacgg caggatgaat aagcagcgcc cacagaagat tccccagaaa caggacaatc
    26101 gtcagtttgg caatatagac agcgggcaaa ccgacaaaat aaaaatccgt cacgaccagc
    26161 cagggcaact gacaggcaaa agccagccca accgacggta tgactttacc ggtttttctg
    26221 aagaacacgc ccgtcatcaa tcccagcaag gcattaccga tgagcaggta gggattgccg
    26281 agcaaaatcg ctgagtaaag cgaaccggtg ataccggctg ccaggccgcc cgccggaccg
    26341 aagcaaacgg ccgccagaaa gattgccgcc ggaaagaaat ggattttcca gtgatgcgca
    26401 aacgttatat taatcaggct caggacacag ggcagcaaaa ccagcaggcc gaaagccaga
    26461 gcgattcgtc gaaaagatct gatgccgttg atcgttacag aggaattcat cacgtcaggc
    26521 cctccctttt ttgtttttga ttctacacga tgagaacttt tttgaacagt ccatttttgc
    26581 gttggatcca tgaaatttcc ggcagaaaat ccgatctaaa aagatgtcat ataaatccgg
    26641 acgaactgcc ggaattttga tcttgacata ttatgagcat atgcttataa ttattattag
    26701 aggagtttca ggaaatgccc agaccgaaat gtcatcgtca aatatgcgga atacctgata
    26761 aaaattattt caaaccgcgg ggtatcccta cggcggatct ggaagaagtg gttctgagcc
    26821 ttgacgaatt tgaagccgtt cgcctcgcgg attatgaaca gctttaccag gaagaagccg
    26881 cgacccggat gaatatttcc cgccagacat tcggcagaat cgtcgcctct gcgcgaaaaa
    26941 agatcgccga tgtcctgatc aacgccaaag cgctgaaaat tgaaggtgga aaagtaacat
    27001 taaacgaaat cgctttctgc cgccggggca aaggctcccg gcttagaaaa aatcacccat
    27061 cattataaaa ggagaactgt atgaaaatcg cactgccgtc ctatcaaaac caggttgacg
    27121 aacatttcgg tcattgtgag tatttcaccg tattcacgac tgacgacaac aaaaatattc
    27181 tctcggaaga aattatcaca ccgccgccgg gatgcggctg caaatcggat atctccttga
    27241 tcctggccca gatgggcgtc aaatacatgc tggccgggaa tatgggacag ggcgccgtaa
    27301 acgttctggg cgcgaacggc attgaggtgc tgcgcggctg ttccggaaac gtcagggaag
    27361 tggttcagaa atggctcgcc ggctcgcttg ccgattcggg gctatcctgc tcgcagcacg
    27421 aacacacctg ccacgagcac taattggaac agcaagataa acgccggcag aacaggccgt
    27481 ccgggcatat ccttaccctt gtccggcaat ggccgaactg accgatgcca tacaaacggg
    27541 aggaagaaac caaagtggaa agcaagaaaa cgtgtgactc atgcagcgat gcgggctgcc
    27601 cggcgaagca acaggaacaa aatgaaaacc aggaagcgta tgcggaccga cgccgtctgc
    27661 aggccaggct ttgccgcatc cggcataaaa tcgtcgtgct ctccggcaag ggcggcgtcg
    27721 gcaaaagcac ggtagccgtc aacctggcga cagcgctcgc gctccatgga ctgcgcgtgg
    27781 gtttgttgga catggacatc cacggcccga gcgttcccac catgctgggg ctggaaggtg
    27841 aatcgctcaa aggcaatccc ggcgaacttt ttccggtgga attcggcggt ttgaaagtca
    27901 tgtcgc
//
LOCUS       DAOSRM010000016        27618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1920_length_27618_cov_114.074919, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000016 DAOSRM010000000
VERSION     DAOSRM010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 27618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27618
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1920_length_27618_cov_114.074919"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1713
                     /locus_tag="PLA58_03015"
     CDS             214..1713
                     /locus_tag="PLA58_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR61798.1"
                     /translation="MNLEKKTKKELVDLIASLQKRLSAQEHTQLHVTSEKDLYKILAH
                     SSQVGCYIMQDGQFQFINSHFLDYTGYPEKKLLKMSPPSLILPEDRRRTAKNAIMMLK
                     GQRFSPYEFRIVTKDGRIKWIMETAMSITFKGRRAILGNSMDITERKEAGRRLEELEA
                     LESSILDAIPQAVVGLHERRINFANHAVKMIFGWHREELIGKSIRMIYRNEEEADKIG
                     EIFYRNLEHQRTYETEFPCRHKDGREILCRMKASRIGEKLREKRVVVTYEDITQEKKA
                     QEELERSREELRNLSIHLQSVREEECARVARKIHDELGQSLTALQMDLSWLLNRVPSG
                     SKAIREKTQNMSGLVDATIESVHNITTELRPSLLDDLGLPAAIEWQAADFQKRSGIRC
                     QAKIQCLKGTFDKQLATAVFRIFQETLTNIARHAKATLCKVTLTETEKELCLDVTDNG
                     IGISQWQVDSPRSFGMIGMRERAHLWCGTVQVQNVKPSGTTVRVLIPLQ"
     gene            1729..2361
                     /locus_tag="PLA58_03020"
     CDS             1729..2361
                     /locus_tag="PLA58_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR61799.1"
                     /translation="MIRILVADDHAVVRQGMIQILADVKDMLVKDEAQNGAETLDKVL
                     KYEYDVVLLDISMPGRSGLEVLEDIKAQRPKLAVLILSMHPEEQYAVRALRAGASGYL
                     TKASAPQELIGAIRKVAGGGKYVTASLAEKLAEELDIDTRKLPHERLSNREHQVMMML
                     ASGKTVSDIADELCLSVKTISTYRTRVLAKMGMKKNAELTLYAVHNKLIN"
     gene            2596..5253
                     /locus_tag="PLA58_03025"
     CDS             2596..5253
                     /locus_tag="PLA58_03025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR61800.1"
                     /translation="MIKGSSTSQRQKAAASPDGKLLSDERSEIIHQAVRDNRSYLMEH
                     ESKEILEGAGIATTGYLVARSEDEALALCEKIGFPVVMKIVSPDVVHKSDAGGVRLNL
                     QSADDVRKAYRGMMDAFKYQHIEGITIQRMAAPGIEAIIGVTRDPSFGPLIMFGLGGV
                     FVEVLRDVSFRILPITEKDAAEMIEEIRGSDILKGYRGRPVDLQSLQQLLLKISRLIE
                     ENPEIHELDLNPLFLYSEGYIAVDARMFVREPAQTETTATGAKNLHDFFYPKSIAVIG
                     ASDVKGKLGYNVFCNLVSHGYPGKLYPINPGKETVMGVRAYKSIRDVPDPVDLAIVIV
                     PAKAAEAAIEDCCTKGVRFVVVEAAGFAESGKEGKEAQARIEAVIRKHGCRVLGPNCS
                     GVINTHHNMVQSIGLLGDLKKGNVGMVAQAGVYAAGILTGLSNVLDFGIVATIGNKMD
                     INETDILEYLSDDDHISVIVMYMEDIRSGKRFVDVASRAALRKPVIVLKSGRTEAGKK
                     AVSSHTASLAGDDEVNNAAFKQSGVIRARDNEHLFALTRAFSKQPIPKGNGVMVITYT
                     GSLGVAATDMLYLSGMRLAELEPYFQQRLKNALPEFANIGNPIDCSFSMTPEQVKNLI
                     EIGVESGDVQSFIVVIQGEILGSFVDVMKSIDYKEKPAACVVACKEFMIHDVVKMEQA
                     GFPVYSTAEMAAEVLGQMYAYGVRRRSAMTDSIARHLTKDTLSVDNMPVRLRLINPKD
                     IALWTDFVNSCSPKSLWLRFLSPFSATPERAQRFCNVNPEEEVALVAEARMGDQHKFL
                     GIARLIKNKRCEGEAEYAIIISDPWQNKSLGITLSEQCIELAKKEGYKTIRAETLQEN
                     YAMIRIFRRCAFNFDGKDENMVSMTLNLS"
     gene            5298..5879
                     /locus_tag="PLA58_03030"
     CDS             5298..5879
                     /locus_tag="PLA58_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR61801.1"
                     /translation="MSSTRNQEDLAQYETRLALKNGKTVLVRPVLQTDEPLIVDLLNR
                     LSSDSIYLRFLRPVRTLPKELLFHLTHLDYQSNFALAAVIREDEKDAVIAVARYGFDP
                     REHLTDFAIVVRDDWQHFGLGKQMLSRIFAIGREQGISRFVSIVDSTNISMKRMLRGL
                     DCAVNYSYEKGFTQVEVLVEEQKAVPRFRGTAF"
     gene            complement(5930..7909)
                     /gene="acs"
                     /locus_tag="PLA58_03035"
     CDS             complement(5930..7909)
                     /gene="acs"
                     /locus_tag="PLA58_03035"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOR61802.1"
                     /translation="MAEANEQDFYDKVFPVPDRVREKSYIKSRAEYDKLYKESITDPN
                     AFWAKMATERLTWFKPFDKNKVSDWSFDAKNVHSKWFEGGKLNVSYNCLDRHLATKKN
                     KAAIIFEGNDPNDWKVYTYFDMYREVNKFANVLKSLGVKKGDRVTIFLPMIVELSIVM
                     LACARIGAVHAIVFGGFSAEALRDRINNSGSAVVITCDGTYRGAKAVPQKDTCDKALT
                     QCPGVKTLVVVKRTGTAVTMKEGRDIWYEELMAKADRYCKPEEMDAEDPLFILYTSGS
                     TGQPKGVVHTQAGYLLYASMTQQLAFDVREEDTYWCTADIGWVTGHSYVVYGPLCNGH
                     SSILFEGVPNYPDFGRFWAVVEKYNVNNFYTAPTAIRSIAKEGDKWVDMHDISSLRVL
                     GSVGEPLNPEAWWWYYNKIGGGRCTISDTWWQTETGGHMILPLPGAIDIKPAKAMVPF
                     FGIVPALMDEEGKEITGPGKGALCIKNSWPGMARGLWDDKEGRFVENYFSQFPGYYFS
                     GDGAERDADGDYKITGRVDDVINVSGHRLGTAEVEAALTSHPDVAEAAVVGFPHDIKG
                     QGIFAYVTLNNNVEKTDELKKALIAHVRKVVGPIASPDQIQWADGLPKTRSGKIMRRV
                     LRNVAAKTFGDFGDTSTLADPAVVDDLVANRKKLG"
     gene            complement(8332..9156)
                     /locus_tag="PLA58_03040"
     CDS             complement(8332..9156)
                     /locus_tag="PLA58_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR61803.1"
                     /translation="MALFTIDQKKCRRDGLCQAACPAQIIIQEDKKSFPTLLENGEEF
                     CINCGHCVAVCPHGAFLLSTMPLSACPPMDGGLLPDAPALKHLLLARRSIRRYKKTPV
                     SRKLLEELVDVARYAPTGSNKQQVWWTVISKPEEVRRMGAMVIDFIKIMLPLTPDEAQ
                     VRRFQRIVLAWENGRDRIMRGAPHLMVAHSPAEVAFPAADCAIALTYLELYAFARGLG
                     TCWAGYFTGAANVHEPLVRALDLPPGHQCQGAVMLGYPQYRYSRIPMRNQAQVTWR"
     gene            9297..11678
                     /locus_tag="PLA58_03045"
     CDS             9297..11678
                     /locus_tag="PLA58_03045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR61804.1"
                     /translation="MAKLTLDEWIARLKKNKSFSANAAAVVEIPARPGDWEDYPSWVS
                     PHLRDVLVKRGMERLYKHQAQAVRFIRGGRDVVLVTPTASGKTLCYNLPVLQSILDEP
                     ETRALYLFPTKALAQDQMHEAHGLITDLQADIKTFTYDGDTPDDARRAIRRQGHIVVT
                     NPDMLHAGILPHHTKWQKLFVNLKYVVIDELHIYRGIFGSHFANVIRRLVRICRFYGA
                     NPVFICCSATVANPREHAEKILERPVALLDKSGAPAAAKTFVLYNPPIVNRELGIRQS
                     AMTPARKIASDLIANHIQTIVFATSRLNVEVLTKYLKDKFVKTKPLDDHFVTGYRGGY
                     LPNLRRDIESGLRTREVMGVISTNALELGIDIGNLEACIMAGYPGSIASTWQQAGRAG
                     RRTGRSLAILIARSNPLDQFIMENPDYFFSLSPEHCRINPDNLLILLHHIKSAAFELP
                     FEKGEKFGGENLEEFLQYLEEKGVLHQSGDRWHWAAESYPADEVSLRAINPENVVVVD
                     ATDAGNHKVIAEVDWDSAFTAVHDEAIYMLASEQYHVDRLDLERKKAYVRRVDADYYT
                     DAMTYTNVRVIDSFDHHRTGGAIVEHGEVQVVRKVVGYKKIKFYTSENLGYGDVNLPE
                     KDMHTTSYWFTIPRDRLRTLDFTPAEVIDGLSALAYTLQHLSAMFLMADTHDIDRSLG
                     DKSGEWFVSQGKTGRVITSFSGGRPQAASGPDGARIDEFDPTIFIFDAYPGGIGFSEL
                     LYHEHANLLSAARSLIEKCPCAFGCPSCVGPTLEVGKTAKAIVPKIIDLVLNQ"
     gene            11675..13000
                     /locus_tag="PLA58_03050"
     CDS             11675..13000
                     /locus_tag="PLA58_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOR61805.1"
                     /translation="MKSIDKLKRLTGESVSPKPENERPAVPPSSEKQFRIDDLRRRID
                     GVVARSAARARAAQDEARARGNRALCEVLEGEERENRHGRFFLAGGFLSGASRHGHRS
                     ILEAFDIDMTAAGMLANNPVLGAYHPSDALFLDTETTGLSGGTGTMAFLIGLGWFEEG
                     RFHVRQILARDFSEEPAALACLQEIAAQKKFLVTFNGKAFDVNLLATRFILNRMPSSL
                     HELPHLDLLHPARRLLGHRLENCRLATLEADILGVEREGDIPGWEIPQRYFDWLRRRD
                     PRLLADIFEHNRLDVISMATLTAHLVDLLNARALKQHAHPDDYLAAARLLLKKAPIAS
                     VKKILELLGEDACAGMSFASKKKLANLCKRAGRLDEAVRLWRQIIERNPRDFYAVSEL
                     AKHLEHRARDYARAMALIETALAGGNLFSEEEKDSLGRRLKRLTARIQS"
     gene            13203..14546
                     /locus_tag="PLA58_03055"
     CDS             13203..14546
                     /locus_tag="PLA58_03055"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR61806.1"
                     /translation="MFNRNILNILKEWKIRPQRKPLVIRGARQVGKTSAVHQFGRATF
                     DNYIYINLELEDHAALFHRKLPIKDLIELIQLKFNQKASPGTTLIFIDEIQNSGIAMN
                     QLRYFYEDMPDIHVVAAGSLLEVKMKSEGFSFPVGRVEYCYMHPVTFDEFLTALGEVQ
                     ALNYIHAVKADVAVPEEIHALLMKKFSEYLLVGGMPEAVARYAKTRSYIDVDPVYESI
                     LTGLRDDVSKYAGMAKTKYIQHILEHASKYVGLPIKYEKFGESNFRSREMSEAFDALE
                     KAMIIHRVFASTSRQKPLVSNLKKSPKLLHLDIGLVNYQADLRMDIAMTEDVNAVYSG
                     QVGEQIAGQTLLAMNMRKQTNLHYWRREQKGSTSEVDYLMAINDRLIPVEVKSGKTGT
                     LRSLHNFMEESESDLAIRAYSGRMTTEKITTPGGKSYALLSMPFYLLHRIEELLS"
     gene            complement(14767..16140)
                     /locus_tag="PLA58_03060"
     CDS             complement(14767..16140)
                     /locus_tag="PLA58_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOR61807.1"
                     /translation="MKDTLEILIVDDDAVHRRAVRLNMAGWGYSVTEADDGEEAVRQV
                     RVRTFDLILMDIRMTKMSGLEALEQIKKIQPAVPVIIMTAYASVETAVSAMKMGADDY
                     LTKPLNYDELQIKIARTAERRRLKSENEYLKERLGEKFDRRNLIGQSKAMIRLLEMVE
                     QVAATQATVLITGESGTGKEMIANAIHFNSSRREASFVKINCAALTETLLESELFGHE
                     KGAFTGADRRREGKFVQADGGSIFLDEVSEMSPAMQVKLLRVLQERELTRVGGQDVVR
                     VDVRLIAASNKDLKKEIREGRFREDLFYRLNVVALALPPLKERAEDIPLLAQHFLKLF
                     AERNAKAIFGFTPRAMQKLAAYAWPGNVRELMNAVERAVVLSRSDTVDEDELVFPMAD
                     QCLSPDASRMAAPTAGLPLEAVEKQSILEAMKAAGGNKSEAARRLGITRKTLRKKLEK
                     YESANEG"
     gene            complement(16137..17825)
                     /locus_tag="PLA58_03065"
     CDS             complement(16137..17825)
                     /locus_tag="PLA58_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR61808.1"
                     /translation="MPEKKKRKVRFDISVWVVISAVAVLAVILGMMLLMQFQQAQNQA
                     RILLIEKGTTLIHSFESGLRLLPEQMEEGFYLQKLLMETAQQPDVDYLMVTDGEGHIL
                     ADSDPSMLGLSYGLDLDVKKIAQSREIFWRQTANPQGAGTFEVYRGLSPAARNAGKEG
                     LIVYVGFNMAKIEKASREDALRTAVTALVLVLIGSLAIVSLFLVQAYRLARVSLSRVT
                     VFSEALVKNMPIALLAVDREGKIAACNDQAKGLLTGSPAEAVGREASRVLPEPFLRLL
                     EVVQGRTGPVEEDILWASETGEQTWEVVAAAFADDEDQEGRILLARNVTGVRQMEKEV
                     ARSRHLNAIASLAAGVAHEIRNPLSSIKGFAVYFKKRLAGNREDEETAEIMIAETERL
                     NRVISQLIEFARPLVLKREPADLEDLVRQTVRLVTAEAEQNGVAVEIRAGKGLPRADV
                     DPDKVRQVLLNIFLNALAAMPGGGRLSILLNRREEFVEVEVSDTGEGIPRESLPRIYD
                     PYFTSKPAGTGLGLAVAQKIMDAHGGAIQVESSEGTGTRVSLRFPLMEKEGMEE"
     gene            18036..18548
                     /locus_tag="PLA58_03070"
     CDS             18036..18548
                     /locus_tag="PLA58_03070"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOR61809.1"
                     /translation="MKKITLTLTAVLAGLLLTTSAFAWGPGPGKGRGYGPCGNASLER
                     LNLTDEQNVKIEALQDAHYKATKAVREKIFDKSVELRRLWLQANPDKGKISAAQKELR
                     TLRNEMEDKATALRFEIDKVLTPEQKDKLANLPWGRKTGFGPRGGMRGDCGFGAGQGP
                     GPGLGMGICQ"
     gene            18879..20429
                     /locus_tag="PLA58_03075"
     CDS             18879..20429
                     /locus_tag="PLA58_03075"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOR61810.1"
                     /translation="MKKDKNHVYIFDTSLRDGEQSPGNSMNTEEKLLLARQLERLGVD
                     IIEAGFPIASEGDFDAVRQIAREIRKSQVAGLARANKQDIDRAWQAIRQAAHPRIHTF
                     ISSSDIHLKYQLKKSRAQVLKEAVAAVKLAKSYTPNVEFSPMDATRSDRDYLAQMVSA
                     VIDAGAVTVNIPDTVGYTVPEEFGSLIAFLFDRVKNMGDTIISVHCHNDLGLAVANSL
                     AAIRAGARQVECTINGIGERAGNTAMEEIVMALATRKDMFGLSTQIKTDQIYKTSRLL
                     TQITGIPVQPNKAIVGANAFAHESGIHQDGLIKEKITYEIMTPQSVGISDSHIVLGKH
                     SGRAAISQHLKKMGYLLNEDQINRVAAKVKDLADVKKDIYDEDMEAILYEEIYRGEEK
                     YNLIYLNVVSGNVAIPTATMEMQVGKDVIRDAGFGNGPVDATFAAIRNITKTNYLLLK
                     YAVNAITGGSDAQGETMVQLKYNGHSVIGRGAHPDVIVASAKAYINALNRLEFLKDNA
                     KKVKLELS"
     gene            20616..21875
                     /gene="leuC"
                     /locus_tag="PLA58_03080"
     CDS             20616..21875
                     /gene="leuC"
                     /locus_tag="PLA58_03080"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOR61811.1"
                     /translation="MGYTITEKILMAHTDLKEIAPGRLINARVDIALGNDITAPIAIR
                     EFRAAGGKKVFDRDRVALVLDHFTPNKDIDSAQQCKTVREFAFEHGVTHFYEGGQVGV
                     EHALLPEKGIVLPGDLVIGADSHTCTYGALGAFATGVGSTDLAAAMLTGELWFKVPES
                     IKFVIYGKLQKWVSGKDLILHIIGRIGVDGALYSAMEFTGETIRRLPMADRLSMANMA
                     IEAGAKNGIFPPDEITGEYVKPRAKRPYTFYTSDPDAVYAQTIEIDASQLELQVAFPH
                     LPSNVRGISQAGGVKIDQSLIGSCTNGRIEDLRVAAGILKGRKAAPHVRLIVVPATPL
                     IYRQALREGLLEIFMDAGAVISPPSCGACLGGHLGILAEGEKSVATTNRNFVGRMGHP
                     KSEVYLAGPAVAAASAVLGRIASPEEL"
     gene            21895..22380
                     /locus_tag="PLA58_03085"
     CDS             21895..22380
                     /locus_tag="PLA58_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOR61812.1"
                     /translation="MILEGRVWKFGDNIDTDAIIPARHLNTSDPAQLARHCMEDADPA
                     FITRMKPGDIIVGGENFGCGSSREHAPIAIKAAGISCVVAKSFARIFYRNAFNMGLPI
                     FESRDLADAIAEGARISLDSAQGSITVEGLDRKFSISPIPPFMEQLIADGGLMKHIAG
                     K"
     gene            22388..23485
                     /gene="leuB"
                     /locus_tag="PLA58_03090"
     CDS             22388..23485
                     /gene="leuB"
                     /locus_tag="PLA58_03090"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOR61813.1"
                     /translation="MTKQNLKIAVFAGDGIGPEIIREALKVLRLVADRRGISLTLTEG
                     LIGGSAYDACGAPFPDESLMLALQSDAVLLGAVGGPKWEGLDYSVRPERALLGLREKL
                     GLYANLRPVLVFDELADASPLKKNLVQGIDILILRELTGDIYFGRPRGVTKDQSGRRT
                     GVNTMIYTEDEIRRIALRAFELARGRRKKLLSVDKANVLETTELWRQVVTETGRGYPD
                     VALSHMYVDNCAMQLIRNPSQFDVIVTSNLFGDILSDEAAMLTGSIGMLPSASLGDGK
                     ALYEPIHGSAPDIAGKNIANPLATILSVAMMLRHSLDRPAEAALIEQAVKSVLRDGRR
                     TPDIAPAGGRADTTEEMGDAVVRKLSTLLHS"
     gene            23521..24141
                     /locus_tag="PLA58_03095"
     CDS             23521..24141
                     /locus_tag="PLA58_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61814.1"
                     /translation="MPNTVTILDHIIIAPPVAKIYSRLGFKKKITSLSSDHRQETDRY
                     MEEAASIIRLQGSFLRLSIRKNDGLTVALEGRTTFRSEKLCAFLGKSRDAVLMGATAG
                     KPIMDAIGEKTAQGNLTAAVVYDATASEMTDAALDWIMNYVNGRLRREGRRLLPRRFS
                     AGYADFSLDNQKAMHKKLQMEKIGVSITPDCILHPEKSVTAIGAIP"
     gene            complement(24189..25433)
                     /locus_tag="PLA58_03100"
     CDS             complement(24189..25433)
                     /locus_tag="PLA58_03100"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61815.1"
                     /translation="MLDDHLNFFYYIVLFAAYCFAGWVAEVIYRSVTQRKFVNAGFLF
                     GPFLPIYGIGAFLVLALERVLQDWPMAVRFAAFGLVITLLEYSAGYLSEKIFKLKLWD
                     YSEKRFNLHGYVCLSFSLLWTLLATGFALFVHPEAIRRAAMLPAGLVQIAAIVFLVYF
                     WIDYTFSVISLAAFRKGVAYLYEEYFNLSSLEIEDILKSFQRLREAFPDLNKYVNQNI
                     NRRIRIKVGSFLKPVLEKIFQELDGRKPFEAEYYETVSDILRHEEFRKLKDYFHHNSS
                     IYRHVHDVAYLSYRISKFLGLDYRSTARGALLHDFFLYDWRNHDAPDLPREKFHGLEH
                     PKIAVANARKYFSLNDIEEDIIRKHMWPLTLVPPKYKESYIVSFADKYLSSKEFISEY
                     KKRIGARRSKIPRGKHSSARKS"
     gene            25578..26507
                     /locus_tag="PLA58_03105"
     CDS             25578..26507
                     /locus_tag="PLA58_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOR61816.1"
                     /translation="MIKDCFIAALKGMGMGAANVIPGVSGGTIALITGIFQRLIDALK
                     SFDLQAFKLFFRGRWRAFARRTDFFFLLAVFAGAAASILSLARLFRFLFDHYPVFIWS
                     YFFGLILASVFFVGKTIGRRTPAVVLVFILGAAIAATISLLRPAAQNDSFWYLVICGV
                     VAICSMILPGISGSFVLILMGNYELIVIDAVNALDWRILLPVALGAAVGLVAFSHFLS
                     WIFRKFRAETISALTGFILGSLITLWPWKTAVWRLNADGAPVVRDGKKLAEGYTYSLP
                     PPDEQFWWALAVMAAGVLTIWATEKLAVRNDPP"
     gene            26548..27495
                     /locus_tag="PLA58_03110"
     CDS             26548..27495
                     /locus_tag="PLA58_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOR61817.1"
                     /translation="MEKIKLGISTCLLGENVRYDGGHKLDRFLRDTLGRYVDYAPVCP
                     ETECGMGIPREAMRLEGNPDAPRLVTRSTRIDKTEMMVSWAKKRIARLASEDLCGFIF
                     KSDSPSSGMERVKVYDEKGMPAKTGVGIFARMFMDAFPLLPVEEEGRLHDPGLRENFI
                     ERIFALRRWREVRGGKSPRGALVDFHTRHKLLLLSHSTKLYREMGKLVAEQKRHSLQD
                     LWSRYEALLMEALKLKTSPKKHVNVLMHMMGYFREKLSTDEKKELLEIFEHTAQGFVP
                     LIVPLTLIGHYVRKYNEPYLKEQVYLNPHPLELQLRNHV"
BASE COUNT         6223 a         8026 c         7748 g         5621 t
ORIGIN      
        1 atattgtcac cctgagcttg tcgaagggca ttattcacct tgcggcgcgt tcggggaccg
       61 cgccctacag ccattgccca tcgccccggg cccattaccc atgacctttc tttccactca
      121 tcactttcca ctttcccctt ttttactaaa gtatttattt ttcccttcaa ttgtgtttag
      181 atgccgccag ttcaatccaa agcgggcaaa cgaatgaatc tggagaaaaa aacaaaaaaa
      241 gagttggtcg atctgatcgc gtccctgcag aagcggctgt cggcccagga gcacacccag
      301 ctgcacgtca cgtcggaaaa agacctgtac aagattctgg cgcacagctc tcaggtcggc
      361 tgctacatca tgcaggacgg acagtttcaa ttcatcaatt cccactttct ggattacacg
      421 ggctatcccg aaaaaaagct tttgaagatg agcccccctt cgctcatcct gccggaagac
      481 cggcgacgaa ccgccaaaaa tgcgatcatg atgctgaaag ggcagcgctt ttccccctat
      541 gaatttcgga ttgtcacgaa agacggacgg atcaagtgga tcatggaaac cgccatgtcc
      601 atcactttca aggggcggcg ggccattctg ggcaattcca tggacatcac cgaacgcaag
      661 gaagccggac ggcgcctgga ggagctggag gcgctggaat cgtcgattct cgacgcgatt
      721 cctcaggccg tcgtggggct gcacgagcgg cgaatcaatt tcgccaatca cgccgtcaag
      781 atgatcttcg gctggcaccg ggaggaactg atcggcaaga gcatccgaat gatttaccgc
      841 aacgaggaag aagcggataa aatcggtgag attttttaca gaaatctgga gcatcagaga
      901 acctatgaaa cggagtttcc ctgccggcac aaggacggac gggaaatcct ctgccgaatg
      961 aaagcctccc gcatcgggga aaagctgcgc gaaaagcgcg tggtggtgac ctacgaggac
     1021 atcacacagg agaaaaaggc gcaggaggag ctggagcgct cccgcgaaga actgcgcaac
     1081 ctttccattc acctgcaatc ggttcgcgag gaggaatgcg cgcgcgtcgc ccgcaagatt
     1141 cacgacgagc tgggccagtc gctcaccgcg ctgcagatgg acctgtcctg gctgttgaac
     1201 cgtgtgccct ccggatcaaa agccattcgg gaaaagacgc aaaacatgtc cggcctggtg
     1261 gacgccacga ttgaaagcgt gcacaacatc acgacggagc tgcgccccag tctgctcgac
     1321 gacctgggcc tgccggcggc catcgaatgg caggccgcgg attttcagaa gcgctccggc
     1381 attcgctgcc aggccaaaat ccagtgcctc aaaggcacct ttgacaagca gctggccacc
     1441 gccgtcttcc gaatttttca ggaaaccctc accaacatcg cgaggcacgc caaagcgacg
     1501 ctctgcaagg tgacgctgac ggaaacggaa aaggagcttt gtctggacgt gacggacaac
     1561 gggatcggca tatcgcagtg gcaggtggac tccccccgat ccttcggcat gatcggcatg
     1621 cgcgagcgcg cgcatctctg gtgcggcacc gtccaggtcc aaaacgtcaa accgtccgga
     1681 accaccgtgc gggtgctgat cccgcttcag taaaggagaa ctgcaaacat gatacgcatt
     1741 ctggtcgccg atgatcatgc cgtggtgcgg cagggtatga tccagattct cgcggacgtg
     1801 aaggacatgc tggtcaagga cgaagcccag aacggcgcgg aaacgctgga caaggtcctg
     1861 aagtatgaat atgacgtggt cctgctcgac atctccatgc cggggcggag cgggctggag
     1921 gtcctggagg acatcaaggc ccagcggccc aaactggccg tgctgatttt gagcatgcac
     1981 cccgaggagc agtacgcggt tcgcgcgctc cgggcgggcg cgtcgggata tcttacgaaa
     2041 gccagcgcgc cgcaggagct gatcggcgcc atccgcaagg tagcgggcgg cgggaagtac
     2101 gtcacggcgt cgctggcgga aaagctggcc gaagagctgg acatcgacac ccgcaaactt
     2161 ccccacgaga ggctctccaa ccgcgagcac caggtcatga tgatgctggc ttccggcaaa
     2221 accgtttccg acatcgccga cgaactgtgt ctgagcgtga aaaccatcag cacctaccgc
     2281 acccgcgttc tggccaaaat gggcatgaaa aaaaacgcgg agctgaccct ttacgccgtc
     2341 cacaacaagc tgatcaacta gaatcccacg gcgccggttc tcctcttttc tgaatcattt
     2401 cagggacctt caacacactc cggccttcct ctcgtccgac aggccgtcgg acaaaatccg
     2461 tcaatctttc ctgtttcgtt ccgacaaaac attcagcgcc gggccgatag gatccttttt
     2521 tttcctgtgc tacatccccc tcacagaaaa cggcaaaaat cgcggccgga aaccatcacg
     2581 gctcaaggag ggcgtatgat aaagggatct tcgacatcac agaggcaaaa ggcggcggcc
     2641 agccccgacg gcaaactgct cagcgatgaa cggtcggaaa tcatccatca ggcggtgcgg
     2701 gacaaccgca gttatctcat ggagcacgag agcaaggaaa ttctcgaagg cgcgggcatt
     2761 gcgaccacgg gatacctggt ggctcgctcc gaagacgaag cgctggccct ttgcgaaaaa
     2821 atcggcttcc ccgttgtcat gaaaatcgtt tcgccggacg tcgtgcacaa atccgacgcg
     2881 ggcggcgtcc ggctgaatct ccaatccgcc gatgacgtgc gcaaggccta ccgcggcatg
     2941 atggacgcct tcaaatatca gcatatcgag ggcatcacga tccagcgcat ggccgccccc
     3001 ggcattgagg ccatcatcgg cgtgacgcgc gaccccagct tcggcccgct catcatgttc
     3061 ggcctgggcg gcgtgttcgt cgaagtgctg cgcgacgtgt cgttccggat tctgccgatc
     3121 accgaaaagg acgcggcgga aatgatcgaa gaaatccgcg gctcggacat cctcaaagga
     3181 taccgcgggc ggccggtcga tctgcaatcg cttcagcagc tcctcttgaa gatctcccgg
     3241 ctgatcgagg aaaacccgga aattcacgaa ctggatctga atccgctgtt tctctattcc
     3301 gaaggctaca tcgccgtgga cgcccggatg ttcgtccgcg aaccggcgca gacggagacc
     3361 accgccacgg gcgcgaagaa cctgcacgat ttcttctatc ccaaaagcat cgccgtaatc
     3421 ggcgcttcgg atgtcaaggg aaaactgggc tacaatgtgt tttgcaatct ggtcagccac
     3481 ggctatccgg gaaagctcta cccgatcaac cccggcaagg aaaccgtgat gggcgttcgc
     3541 gcctacaagt cgatccggga cgtgccggat ccggtggacc tggccatcgt aatcgttccc
     3601 gcgaaagcgg ccgaggcggc catcgaggac tgctgcacaa agggcgtccg gtttgtcgtt
     3661 gtggaagcgg cgggattcgc ggaaagcgga aaagaaggaa aggaagccca ggcgcgcatc
     3721 gaagccgtca tccgaaagca cggctgccgc gtgctgggtc ccaactgctc cggcgtgatc
     3781 aacacccacc acaacatggt gcagtccatc ggactcctcg gggatttgaa gaagggcaac
     3841 gtcggcatgg tcgcccaggc cggcgtttac gccgccggca tcctcacggg cttaagcaat
     3901 gtgctggact tcggaatcgt ggcgaccatc ggcaacaaga tggacatcaa tgaaacggac
     3961 attctggaat acctgagcga cgacgatcac atttccgtca tcgtcatgta catggaagac
     4021 atccgcagcg ggaagcgatt tgtcgatgtc gcctcccggg cggcgctccg caagcccgtc
     4081 atcgtgctga aatcgggacg gacggaagcc ggcaaaaagg ccgtgtcgtc gcacaccgcg
     4141 tcgctggcgg gcgacgacga agtcaacaac gccgcgttca agcaaagcgg cgtgatccgg
     4201 gcgcgggaca acgagcatct cttcgcgctg acgcgcgcct tttccaaaca gcccatcccg
     4261 aaaggcaacg gtgtgatggt catcacctat accggctcgc tgggcgtcgc ggccaccgac
     4321 atgctgtatc tgtccggcat gcgcctggcc gaactggagc cgtattttca gcagcggctg
     4381 aaaaacgctc tgccggaatt cgccaatatc ggcaatccca ttgactgttc tttctccatg
     4441 acgcccgaac aggtgaaaaa tctgatcgaa atcggcgtgg agtcgggcga cgtgcaaagc
     4501 tttatcgtcg tcattcaggg cgaaattctg gggtcgtttg tggacgtcat gaagtccatc
     4561 gactacaagg aaaaaccggc ggcctgcgtg gtggcctgca aggagtttat gatccatgac
     4621 gtggtcaaga tggagcaggc cggctttccc gtatattcca ccgcggagat ggcggcggaa
     4681 gtgctggggc agatgtacgc ctacggcgtg cggcgccgga gcgccatgac ggattccatc
     4741 gcccgccacc tgacgaaaga cacgctgtcc gtggacaata tgcccgttcg gctgcggctg
     4801 atcaatccca aagatatcgc cctgtggacg gattttgtca acagttgctc cccgaagtcc
     4861 ctctggcttc gcttcctgtc gcctttttcc gccaccccgg aacgggcgca gcgtttctgc
     4921 aacgtcaacc cggaggaaga agtcgccctc gtcgcagagg ccaggatggg ggaccagcac
     4981 aagttcctcg ggattgcgcg cctgatcaaa aacaagcgct gcgagggaga agcggaatac
     5041 gccatcatca tttccgatcc ctggcagaac aaatccctgg ggattactct ttcggagcag
     5101 tgcattgaac tggccaaaaa ggaaggatac aaaaccatcc gtgcggaaac gctgcaggaa
     5161 aattacgcca tgatccgcat cttccgccgc tgcgccttca acttcgacgg caaggacgaa
     5221 aacatggtgt cgatgacgct gaatctgtcg tgagaaaggc ggcggccttc ggagccgccg
     5281 ccggccgggt aatgaaaatg tcatcaacca ggaaccagga agatcttgcg caatacgaaa
     5341 cccggttggc gctgaagaac gggaaaacgg ttctggtccg gcctgttctg cagacggatg
     5401 aacctctgat cgtggatttg ctgaacaggc tttcgtccga ttcgatctat ctgcgttttt
     5461 tgagacccgt caggacactc ccgaaggagt tgctgtttca cctcacacat ctcgattatc
     5521 aaagcaactt cgcgctggcc gccgtgatcc gggaagatga aaaagacgcc gtcatcgccg
     5581 tggcgcggta tggctttgat ccccgggagc atctcacgga ctttgcgatt gtggtgcgcg
     5641 acgactggca gcatttcggc ctcggcaaac agatgctctc caggatattt gcgatcggcc
     5701 gggagcaggg gatttctcgg tttgtatcca tcgtcgattc aaccaacatc tcgatgaagc
     5761 ggatgctcag aggactcgac tgcgcggtga actattctta tgaaaaaggg ttcacgcagg
     5821 ttgaagtcct cgtggaggag caaaaagccg ttccccggtt tcggggaacg gctttttaag
     5881 gagggacttt aatgcggaac cattacagcc gctccgcaca agggggtctt tagccgagtt
     5941 tcttgcggtt ggcgaccaga tcgtccacaa cggccggatc ggccagcgtg gaggtatcgc
     6001 cgaagtcgcc gaaggttttc gcggcgacgt ttctcagaac gcggcgcatg attttgccgg
     6061 agcgggtttt gggaagaccg tccgcccact gaatctggtc gggcgacgcg atcggtccca
     6121 ccaccttgcg gacatgggcg atcagggcct ttttgagttc atccgtcttt tccacgttgt
     6181 tgttcagcgt aacgtaagcg aagatgccct gccctttgat gtcatggggg aatccgacca
     6241 ccgccgcttc cgcgacatcc ggatgggaag tcagcgccgc ttcgacttcc gcggtgccca
     6301 gccggtggcc ggacacgttg atgacgtcgt cgacacggcc ggtgatttta tagtcgccgt
     6361 cggcgtcgcg ctccgcgccg tcgccggaga agtagtaacc ggggaactgg gagaagtaat
     6421 tctcgacaaa acggccttct ttgtcatccc acagtccgcg ggccatgccg ggccaggaat
     6481 ttttaatgca gagggcgcct ttgccgggac cggtgatttc ttttccttct tcatccatca
     6541 gcgccgggac aatgccgaaa aacggaacca tggctttggc gggcttgatg tcgatcgcgc
     6601 cgggcagcgg caggatcata tgcccgccgg tttcggtctg ccaccaggta tcggaaatcg
     6661 tgcagcggcc tccgccgatc ttgttgtagt accaccacca ggcttcgggg ttgagcggct
     6721 cgccgacgga ccccagaacg cgcagcgagg aaatgtcgtg catatcgacc catttgtcgc
     6781 cttctttggc gatggagcgg atcgccgtcg gagcggtata gaaattgttg acattgtatt
     6841 tttcgacgac ggcccagaac cggccgaaat ccggataatt gggcacgcct tcaaacagaa
     6901 tgctggaatg gccgttgcaa agcgggccgt aaacaacgta gctgtggccg gtgacccagc
     6961 cgatatcggc ggtgcaccag taagtgtctt cttcacgaac gtcaaaagcg agctgctgcg
     7021 tcatggaggc gtacagcagg tagccggcct gggtgtgcac gacgcccttg ggctgtccgg
     7081 tcgaaccgga ggtgtaaagg atgaacagcg gatcttccgc gtccatttct tcgggtttgc
     7141 agtagcggtc cgctttggcc atcagttctt cgtaccagat atctcggcct tccttcatgg
     7201 taacggcggt tccggtgcgt ttcacaacca ccagggtttt cacgccgggg cactgcgtca
     7261 gggctttgtc acaggtgtct ttctggggaa cggctttggc gccgcggtag gttccgtcac
     7321 aggtaatgac gaccgcggaa ccggagttgt tgatgcggtc tcttaacgct tccgccgaga
     7381 atccgccgaa aacgatggca tgaacggcgc cgatgcgcgc gcaggccagc atcacgatgg
     7441 acagctcgac gatcatgggc agaaagatgg tgacgcggtc gccctttttg acgcccagac
     7501 ttttgagaac attggcgaat ttgttcactt cgcgatacat gtcgaaatag gtgtaaactt
     7561 tccagtcgtt gggatcgttg ccttcaaaaa tgatcgcggc tttgtttttc ttggtagcca
     7621 gatggcggtc gaggcagtta tagctgacgt tgagcttgcc gccttcaaac catttggaat
     7681 ggacgttttt cgcgtcaaac gaccagtccg acaccttatt tttgtcaaag ggtttgaacc
     7741 aggtcagtct ttcggttgcc attttggccc agaaagcatt gggatcggta atcgattctt
     7801 tataaagctt gtcatactcc gcccggctct tgatgtagga tttctccctg actctgtccg
     7861 gcacaggaaa aaccttgtca taaaaatctt gttcgttagc ttcagccata ttattctcct
     7921 tcaaaattat tttttatgcc ttccttttcc gccaaaaagg catgatttct ctttgtgaac
     7981 cggtgatcca gtgtcattgt tcaagacttg aggggggaca cgggacatca tcagtctttt
     8041 caggaggggt acgccccgcg attcgggcct tgttcctgtt tatcagatgc gcgcttctga
     8101 tccaccggcc gcaaaacctt ggggcgtatt acatatggcg gatgcaatac atgtacccct
     8161 tgaaaagcgg gtattaagta cactttttcc cgggcggacg gtatcgggct ccgtccgaat
     8221 ctgttgaagg cgtatatccc acaggatgtc cgtgcttgtc ttacaaaaag gacagaccaa
     8281 aggtgagagg cgatggatta tcggccagga actacgagca tcgagcgatc atcagcgcca
     8341 ggtcacctgc gcctgattgc gcatcgggat ccgggaatag cggtactgtg gataccccag
     8401 catgacggcg ccctggcact ggtgtccggg gggtaaatca agcgcccgga ccagcggttc
     8461 gtggacattg gcggcgcctg taaaataacc ggcccagcag gtccccaggc ctctggcgaa
     8521 agcgtacaat tccagatacg tcagggcaat ggcgcagtcg gcggcgggaa aggccacttc
     8581 cgcggggctg tgcgcgacca tcagatgcgg ggcgcctctc atgattcggt cgcggccgtt
     8641 ttcccaggct aagacaatgc gttgaaaacg ccggacctgg gcttcatcgg gcgtcagcgg
     8701 cagcatgatt ttgatgaaat caatcaccat ggcgcccatg cggcggacct cctccggctt
     8761 tgaaatcacc gtccaccaga cctgctgttt gttgctgccg gtaggcgcgt accgtgccac
     8821 atccaccaat tcctcaagca gtttgcggga gacgggcgtt ttcttgtacc ggcggataga
     8881 ccgccgggcc aggagcagat gcttcaatgc cggggcgtcg ggcagaagcc ccccgtccat
     8941 cggcggacag gcggaaagcg gcatggtgga aagcaaaaaa gcgccatgag gacagacggc
     9001 cacacagtgt ccgcagttga tgcaaaactc ctcgccgttt tccagaagcg tcggaaagga
     9061 ttttttatcc tcctgaataa taatctgcgc cggacaggcc gcctggcaca gaccgtcgcg
     9121 cctgcatttt ttttgatcga tggtgaaaag cgccatgtcc atcccctttc aaattcagga
     9181 atataggcta tttagatcaa ggatcgggca aatgcaacga cttttatttc ccgccggatt
     9241 gcctgttgag atatttcctt ttttattcta aagctgatgg cgtgaaagga tcatccatgg
     9301 ccaaactcac cctggacgaa tggatcgccc gcctgaaaaa aaacaaaagc ttcagcgcca
     9361 acgcggcagc ggttgtggaa atcccggcca ggccggggga ttgggaagac tacccttcct
     9421 gggtaagccc gcaccttcgt gacgttttgg tcaagcgcgg aatggagcgg ctgtacaagc
     9481 atcaggcgca ggccgtgcgc ttcatccgcg gcggccggga tgttgtgctg gttacaccca
     9541 ccgccagcgg caaaacgctt tgctataacc tgccggtgct gcaaagcatt ctggacgagc
     9601 cggaaacacg cgctctttat ctgtttccca ccaaggcgct ggcgcaggac cagatgcacg
     9661 aagcccacgg tctgattacg gatttgcagg ccgatatcaa aaccttcact tatgacggcg
     9721 atacacccga tgacgcgcgc cgggccatcc gcaggcaggg gcacattgtc gtcaccaatc
     9781 ccgacatgct gcacgcgggc attttgccgc accacaccaa gtggcaaaag ctttttgtca
     9841 acctcaaata cgtcgtgatc gacgaactgc acatctaccg cggcattttc ggttcgcatt
     9901 tcgccaacgt gatccgccgg ctcgtccgca tctgccgctt ctacggcgcc aaccccgtgt
     9961 tcatctgctg ttcggccacg gtggccaacc cgcgcgagca cgcggaaaaa attctggagc
    10021 gccccgtggc gctgctcgat aaaagcggcg cgcccgcggc ggccaaaacc tttgtgctgt
    10081 acaacccgcc catcgtgaac cgcgaactgg gcatccgcca gagcgccatg acgccggcgc
    10141 gcaagatcgc ctccgactta atcgccaatc atattcagac gattgtcttt gccaccagcc
    10201 gactcaacgt ggaagtcctg accaaatatc tcaaagacaa attcgtcaaa acgaagcccc
    10261 tcgacgacca ctttgtcacc ggctaccgcg gcgggtatct gcccaacctc aggcgcgaca
    10321 tcgaaagcgg tctgcggacg cgcgaggtga tgggcgtcat cagcaccaac gcgctggagc
    10381 tgggcatcga catcggcaat ctggaagcct gcatcatggc cggctatccc ggctccattg
    10441 ccagcacctg gcagcaggcg ggccgcgcgg gaaggcgcac gggacgatct ctggcgattt
    10501 tgatcgcgcg gagcaacccg ctggatcagt tcatcatgga aaaccccgac tacttctttt
    10561 cgctctcgcc cgaacactgc cggatcaatc cggacaatct gctgattctc ctgcatcaca
    10621 tcaaaagcgc ggcgtttgaa ctgccgtttg aaaagggcga aaaattcggc ggcgaaaacc
    10681 tggaggagtt tctgcaatac cttgaagaaa aaggcgtcct gcaccaaagc ggcgaccgct
    10741 ggcactgggc ggcggaaagc tatccggccg acgaggtcag cctgcgcgcc atcaacccgg
    10801 aaaacgtcgt ggtcgtcgat gcgaccgacg cgggcaatca caaagtcatc gccgaagtgg
    10861 actgggacag cgccttcacc gccgtccacg atgaggccat ctacatgctg gcctccgaac
    10921 agtaccatgt ggaccgactg gacctggagc gaaagaaggc ctacgtccgg cgggtggacg
    10981 cggactatta caccgacgcc atgacgtaca ccaacgtgcg cgtcatcgat tcgttcgatc
    11041 atcaccggac cggcggcgcg attgtggagc acggcgaggt gcaggtcgtt cgcaaggtgg
    11101 tgggctacaa gaagatcaaa ttttacacct cggaaaatct gggctacggg gacgtgaatc
    11161 ttccggaaaa ggacatgcac acgaccagct actggtttac gattccgcgc gaccggctgc
    11221 ggacgctcga tttcacgccg gcggaagtga tcgacgggct ttcggcgctg gcttacacgc
    11281 tccagcacct gtcggccatg tttctgatgg cggatacgca cgacattgac cggtcgctcg
    11341 gcgacaagtc cggcgagtgg ttcgtcagcc agggaaaaac cggccgcgtc attacctcgt
    11401 tctccggcgg caggccgcag gcggcaagcg gtcccgacgg cgcgcgcatc gatgaattcg
    11461 acccgacgat ctttatcttt gatgcctatc ccggcggcat cggcttttcg gagctcctct
    11521 accacgagca cgccaatctg ctttccgccg cccgaagttt gattgaaaag tgcccctgcg
    11581 cgttcggctg tccttcctgc gtcggcccca cgctggaagt cggcaaaacc gccaaggcga
    11641 tagtgccgaa gattattgat ctggttttga atcaatgaaa tcgattgata aattaaagcg
    11701 cctgacgggc gaaagcgttt caccgaaacc cgaaaatgaa aggccggctg tccctccgtc
    11761 atcggaaaaa caattccgga tcgacgatct gcgacgacgc atcgacggcg ttgtcgcgcg
    11821 ctcggccgcg cgcgccaggg ctgcgcagga tgaagcaagg gcgcgcggca accgggcgct
    11881 ttgcgaagtc ctggaggggg aggagcgcga aaaccggcac ggacgtttct ttctggccgg
    11941 gggttttctt tccggcgcca gccgccacgg ccaccgcagc atcctcgagg cttttgatat
    12001 cgacatgacc gcggcgggca tgctcgccaa caacccggtc ctgggcgctt atcacccttc
    12061 cgacgcgctc tttctggaca cggaaaccac gggactttcc ggcggaacgg gcaccatggc
    12121 gtttctcatc ggcctgggct ggttcgaaga aggccggttt cacgtccggc agattctggc
    12181 gcgcgatttc agcgaggagc ccgcggcgct ggcctgcctt caggaaatcg ccgcgcagaa
    12241 aaaattcctc gtgaccttca acggcaaagc ctttgacgtc aacctgctgg ctacgcgctt
    12301 cattctgaac cggatgccga gcagccttca cgaactgccc cacctggatc tgctgcaccc
    12361 ggcgcgccgg ctgctcgggc accgcctgga aaactgccgc ctggccacgc tggaggcgga
    12421 cattctgggc gtagagcgcg aaggggacat tcccggctgg gaaatcccgc agcggtattt
    12481 tgactggctg agaaggcgcg atccccggct gctcgccgac atcttcgaac acaaccggct
    12541 ggacgtgatt tcgatggcga cgctcaccgc gcacctggtc gatcttctga acgcgcgggc
    12601 cctcaagcag cacgcgcatc ccgacgacta tctggcggcg gcgcggcttc tgctcaaaaa
    12661 ggcgccgata gcaagcgtta aaaaaattct ggaacttctc ggggaagacg cctgcgccgg
    12721 gatgtcgttc gcctccaaaa agaaactggc gaatctctgc aaacgcgccg gccgcctgga
    12781 tgaagccgtc cgactctggc ggcaaatcat cgagcgcaat ccccgtgatt tttacgccgt
    12841 ctccgagctt gccaagcatc tggagcaccg cgccagggat tacgcccggg ccatggcgct
    12901 gatcgaaacc gccctggccg gaggaaattt gttttccgaa gaagagaagg attccctcgg
    12961 gcgccgattg aagcggctga ccgccagaat acaatcctaa acctctgcgc ccgctgctgc
    13021 ttcccttcgc caaaacttca atggccgctt tcaaagccgg cctgcaccgg cagacggccg
    13081 tcctcaaaag aatgaaaaaa atgacgagac atggaaaata accctatatt cattacaatt
    13141 aatatgatat atagcctata aaatactaca acaaacatca ataagaggtt attaattata
    13201 acatgtttaa tcgtaatata ttgaatatac ttaaggaatg gaaaataagg ccgcagcgga
    13261 aacctctggt tatccgggga gcccgccagg tggggaaaac atcggcagtt catcagtttg
    13321 gaagagctac ttttgacaat tatatttata tcaaccttga actggaagac catgctgcat
    13381 tgtttcatag aaagttgccg atcaaggatt tgattgagct tattcagctt aaattcaatc
    13441 aaaaggcgtc tcccggcacc acgctgattt ttatcgatga aatacaaaac tccggcatcg
    13501 ccatgaatca gcttcgctac ttttatgaag acatgccgga tattcatgtc gtggcagccg
    13561 gatcgctgct ggaagtgaag atgaagtccg aaggcttttc ttttcccgtg ggaagagtgg
    13621 aatattgcta tatgcatccg gtgacatttg atgaattttt aacggcgctg ggggaggttc
    13681 aggctttaaa ctatatccat gcagtgaaag cggatgtcgc cgtacctgaa gaaatacacg
    13741 ctctgctgat gaaaaagttt tccgaatacc ttctggtcgg cggtatgccg gaagcggtgg
    13801 cacgctacgc aaaaaccaga tcgtatattg atgttgatcc ggtttatgaa tccattctca
    13861 cagggttgcg cgacgacgtt tccaaatatg ccggtatggc gaagacaaaa tatatccagc
    13921 atattcttga acatgcctcg aaatatgtcg gtcttccgat caagtatgaa aaattcggcg
    13981 agtcgaactt ccgtagccgt gaaatgagtg aggcgtttga cgcgctggaa aaagcaatga
    14041 tcatccatcg ggtgtttgct tccacatcca ggcagaagcc gctcgtcagc aacctgaaaa
    14101 aatctccaaa acttctgcat ctcgatatcg gactggtcaa ctatcaggcg gatctgagga
    14161 tggacattgc tatgacggaa gatgtgaacg ccgtctattc cggtcaggtg ggcgaacaga
    14221 ttgccggaca gaccttgctg gcgatgaata tgcgcaaaca gaccaacctt cattactggc
    14281 ggcgcgaaca aaagggttcg acatctgaag tcgattatct gatggcgatc aacgaccggc
    14341 tgattccggt ggaagttaaa tccggcaaaa caggcacact gcgttccctg cataatttca
    14401 tggaagaaag cgaaagcgat ttggccatcc gggcatattc cggtaggatg accacggaaa
    14461 aaattacaac gcccggcggg aagagttacg cgctcttgtc catgccgttt tatctcctgc
    14521 accggattga agagctgtta tcctgatcgg cagttgtccc aacatctgat gtcctgagca
    14581 tggattgcgt tgccgattgg cgcataataa ttttcaaacc aggggtatga aaatactctt
    14641 tactccagcg cattcgattc acttggcggg ttgagcgatt ctgcggaact cttaaagcca
    14701 tttcaaccgg ctgtttaacc gcgagacatt gtaggaagaa gcagggcggt atggcagcgc
    14761 ctgcattcat ccttcatttg cgctttcata cttttccagc ttcttgcgca gggttttgcg
    14821 ggtgatgccc agacggcgcg cggcttcgct tttgttgccg ccggccgctt tcatcgcctc
    14881 cagaatgctt tgcttttcga ccgcctcgag cggcaggccc gccgtcggcg cggccattcg
    14941 ggaagcgtcc ggcgaaaggc actggtccgc catcggaaaa accagttcgt cttcgtccac
    15001 cgtatcggaa cgggacagga ccacggcccg ttcgacggcg ttcatcagct cccggacatt
    15061 tccgggccag gcgtacgccg caagcttttg catcgcccgg ggcgtgaacc cgaaaatagc
    15121 tttggcattc ctttcggcaa acagcttcag aaaatgctgc gccagaagcg gaatgtcttc
    15181 ggccctttcc ttgagcggcg gcagggccag cgcaacgacg ttcagccggt aaaacaaatc
    15241 ctcgcgaaac cgcccttcgc ggatctcctt tttcaaatcc ttgttggaag cggcaatcag
    15301 gcggacatcg acccggacga cgtcctgacc cccaacgcgc gtcagctccc tctcctgcag
    15361 gacccgcagg agcttcacct gcatggcggg agacatttcg ctcacctcgt ccaggaaaat
    15421 gctgccgccg tcggcctgga cgaacttccc ttcccgccgg cggtccgcgc cggtgaacgc
    15481 gcctttttca tggccgaaca actccgactc caggagcgtt tcggtaagcg ccgcacagtt
    15541 gatcttcaca aaggatgctt cccggcggct gctgttgaaa tggattgcgt tggcgatcat
    15601 ttccttcccc gttcccgatt ctcccgtaat cagcaccgtg gcctgcgtcg cggcgacctg
    15661 ctccaccatt tccagaagtc ggatcatagc cttgctctgg ccgatcagat tccggcggtc
    15721 gaacttctcg cccagtcttt ccttcagata ttcattctcg cttttcagcc ggcggcgctc
    15781 cgccgtccgc gcgattttga tttgcagttc gtcgtagttc agcggcttgg tcagataatc
    15841 gtcggcgccc atcttcatgg cgctgacggc ggtttccacc gacgcgtagg ccgtcatgat
    15901 aatcaccgga acggccggct gaattttctt gatctgttcg agggcttcca gacccgacat
    15961 cttcgtcatg cggatgtcca tcaggatcag gtcaaacgtc ctcacgcgaa cctgccggac
    16021 ggcttcttcc ccgtcgtccg cttccgtaac ggaatacccc cagccggcca tgttgagccg
    16081 gaccgctctg cggtgaaccg catcgtcgtc cacaatcaaa atttccagtg tatctttcat
    16141 tcttccatcc cttctttttc catcagcggg aagcgcagcg aaacccgggt ccctgtccct
    16201 tcagagcttt ccacttgaat cgcgccgccg tgcgcgtcca taatcttctg tgcgaccgca
    16261 aggcccaaac cggtcccggc cggcttggac gtgaaatatg gatcataaat ccggggcagg
    16321 ctttcccggg gaatcccttc tcccgtgtcg gaaacttcaa cttccacgaa ttcttcccgc
    16381 cggttcagca ggatcgacag cctgcccccg ccgggcattg cagccagcgc gtttaagaaa
    16441 atattgagca gcacctgcct gaccttgtcc ggatccacgt cggcccgggg aagccccttc
    16501 cccgcccgga tttccaccgc cacgccattt tgctccgctt cggccgtcac cagacgaacc
    16561 gtctgcctca ccagatcctc caggtccgcc ggctcccttt tcagaaccag cggacgggca
    16621 aactcaatga gctggctgat gacgcggttc agtctttcgg tctcggcgat catgatttcg
    16681 gccgtctctt cgtcttcccg attcccggcc agcctttttt tgaaataaac ggcaaatccc
    16741 ttgatggagc tcagcggatt gcggatttcg tgcgccacgc cggcggccag cgatgcgatg
    16801 gcattcaggt ggcggcttcg ggcgacttcc ttttccatct gccggacacc cgtcacgttg
    16861 cgggccagca gaattctccc ttcctgatcc tcatcgtcgg caaaagccgc cgcaacaacc
    16921 tcccaggtct gttcgccggt ttcggaagcc caaaggatgt cctcttcaac cggaccggtc
    16981 cgtccctgaa cgacttccag cagccgcaaa aacggttcgg gcagaacacg ggaagcctcc
    17041 cggcccaccg cttccgcggg agagccggta agcaggcctt ttgcctggtc attgcaggcg
    17101 gcaatttttc cttcccggtc caccgccaga agcgcgatgg gcatattctt gaccagggct
    17161 tccgaaaaga cggtgacccg cgaaagagac acgcgcgcca gacgatacgc ctggaccaga
    17221 aaaagagaca cgatggccag agacccgatc aggacaagca cgagcgccgt cactgccgtc
    17281 cgaagcgcgt cttcccggga cgctttttcg attttggcca tgttgaatcc gacataaaca
    17341 atcaggccct ctttcccggc attcctcgcc gccggggaaa gccccctgta aacctcaaac
    17401 gtacccgcac cttggggatt ggccgtctgc cgccagaaaa tttcccggct ttgcgcaatt
    17461 tttttcacgt ccagatcaag tccgtaggac agtcccagca tggacggatc actgtccgcc
    17521 agaatatgac cttcgccgtc cgtgaccatc aggtaatcca catccggctg ctgggccgtt
    17581 tccatgagca gtttctgaag atagaagcct tcttccatct gctccggaag gaggcgcagg
    17641 cccgattcaa aagaatgaat caacgttgtc cccttctcga tcaacagaat ccgcgcctga
    17701 ttctgggcct gctgaaactg catcaacagc atcatcccca aaatgacggc taaaaccgca
    17761 acggcgctga tgaccaccca gacggaaatg tcaaagcgga cttttctctt ttttttctca
    17821 ggcatgtgtt tttctcccgc agcaccctgg atacaatgga cccatccgga tacccaaatt
    17881 gggtacgcat tatccgtcca atgcgggcaa aaggcaaatc aaatttttac tgatttttaa
    17941 taagatcaat aattacaaat aattaacatc gttggcacat gccttgctct ataaatcggc
    18001 aactgatcag aaataaatga aaaaggagat gcaccatgaa aaaaattaca ttaacgctaa
    18061 cagccgtttt ggcgggcttg cttttaacga cgtcggcctt cgcctgggga cccggcccgg
    18121 gaaaaggcag aggatacgga ccctgcggga atgcaagcct ggaaagatta aacctgactg
    18181 atgaacagaa tgtaaaaatt gaagccctgc aggacgcaca ttacaaagcc acaaaagccg
    18241 tccgggaaaa gatttttgac aaatccgtgg aattgcgaag actgtggctg caggccaatc
    18301 cggacaaggg aaaaatctct gcggctcaaa aggagctgag gacactgaga aacgaaatgg
    18361 aggacaaagc aaccgcctta aggtttgaaa ttgacaaggt tttgactcct gagcaaaagg
    18421 ataaactggc caacttgcca tggggcagaa aaacaggctt tggcccccgc ggcggaatga
    18481 gaggcgactg tggattcggc gccggccagg gtcccggacc cggtttagga atgggtatct
    18541 gtcaatagcc aatttgccca tccctccttt ggaaagggga gcttcggctc ccctttttta
    18601 ttgaaaagaa aaaagttttg acaaatcttg cgttttgcat taacaagaca aacctatttt
    18661 tgcgaatgta taaggaaagc aatgttcagc tttaggacga caacgatttt ggcaagcgct
    18721 cttctgaccc tgcttccggg cctcgccctg ttgcgcctgt tggtcaaggc cgcctgcccg
    18781 ccgtttttag gtcgtcgctg aacgcttcaa atagatgatt acctgaaagg ccgtgggcgg
    18841 aacccacggc ctttttttat acagagaaag gagcccctat gaaaaaggat aaaaatcacg
    18901 tctatatttt tgacacatcc ctccgggatg gagaacagtc gccgggcaac agcatgaaca
    18961 cggaggaaaa gctgcttctg gcccgacagc tcgaaaggct cggcgtcgat atcatcgagg
    19021 cggggtttcc catcgcctcg gagggcgatt ttgacgccgt ccgccagatc gcccgggaaa
    19081 tccggaaatc gcaggtggcg ggactggcgc gcgccaacaa acaggacatc gaccgcgcct
    19141 ggcaggccat ccggcaggcg gcgcatcccc gcatccacac gttcatttca tcgtcggaca
    19201 tccatctgaa ataccagctc aagaagagcc gcgcgcaggt gctcaaggaa gcggtggcgg
    19261 ccgtgaagct tgcgaaatcc tacaccccca acgtcgaatt ttcaccgatg gacgccacgc
    19321 gctcggacag ggattatctg gctcagatgg tttccgccgt gattgacgcg ggcgccgtca
    19381 ccgtcaatat tcccgacacg gtcggctaca ccgttcccga agagttcggc agcctcatcg
    19441 ccttcctgtt cgatcgtgtg aaaaacatgg gcgatacgat catctccgtc cactgccaca
    19501 acgatctggg gctggcggtg gccaattcgc tcgccgccat ccgcgcgggc gccaggcagg
    19561 tggagtgcac gatcaacggc atcggggaaa gggccggaaa cacggcgatg gaagaaatcg
    19621 tcatggcgct ggccacgcga aaagatatgt tcggcctttc cacccaaatc aaaacagatc
    19681 agatttataa aacctcgcgc ctgctgacgc agattaccgg catacccgtg cagccgaaca
    19741 aggccatcgt cggcgccaac gcgttcgcgc acgaatccgg catccatcag gacggcctga
    19801 tcaaggagaa aatcacctac gagattatga cgccccagtc cgtcgggatc agcgacagcc
    19861 atatcgtttt gggcaagcat tccggtcgcg cggccatttc gcagcatctg aaaaaaatgg
    19921 gctacctcct gaatgaagat cagatcaacc gcgtcgcggc aaaggtgaaa gacctggcgg
    19981 atgtgaagaa agacatctac gatgaggaca tggaggcgat tctctatgaa gagatctacc
    20041 ggggagagga aaagtacaac ctgatctacc tcaatgtggt cagcggcaat gtggccattc
    20101 ccacggcgac catggaaatg caggtgggca aagacgtgat ccgcgacgcg ggattcggca
    20161 acgggccggt ggatgcgacc ttcgcggcca tccgcaacat caccaagacc aactacctgc
    20221 ttttgaagta cgcggtcaac gccattaccg gcggatcgga cgcgcagggg gagacgatgg
    20281 tgcaactcaa gtacaacggg cattccgtca tcgggcgcgg cgcgcatccg gacgtgatcg
    20341 tggcctcggc caaggcgtac atcaacgcgc tgaaccggct ggaatttttg aaagacaacg
    20401 ccaaaaaggt taaacttgaa ttaagctgaa aaatgcgata gtgaaaaacc gcgttcccgc
    20461 aaaaaatccg gtgggatacc ttttgtcatg gcgggctgtc caaccctcag gagttcaaag
    20521 gtcggcccct ccggccggcc cggcaaaccc ggttttttgc gaaagcgtcg gcatcatctt
    20581 tataaaacgc attccatcat cagggagctt acagcgtggg atacacgata acggaaaaaa
    20641 ttttaatggc ccacacggac ctgaaggaaa tcgcgcccgg ccggctcatc aacgcccggg
    20701 tcgatatcgc gctgggcaac gacatcacgg cgcccatcgc catccgcgaa ttccgcgcgg
    20761 ccggcgggaa gaaggttttt gaccgcgacc gggtggcgct ggttttggat cattttacgc
    20821 cgaacaaaga catcgactcc gcccagcagt gcaaaaccgt ccgcgagttc gccttcgaac
    20881 acggcgtcac acacttctac gaaggcggac aggtgggcgt cgaacacgcg ctgctgccgg
    20941 aaaagggcat cgttcttccc ggcgacctgg tcatcggcgc ggacagccac acctgcacct
    21001 acggcgccct gggcgctttt gccaccggcg tcggcagcac ggatctcgcc gccgccatgc
    21061 tcacgggcga actgtggttc aaggttccgg agtcgatcaa atttgtcatc tacggcaaac
    21121 tgcaaaaatg ggtttccgga aaagacctga ttcttcacat catcggacgc atcggcgtgg
    21181 acggcgctct ctattccgcg atggagttca cgggcgaaac catccgcagg cttcccatgg
    21241 ccgaccggct gtccatggcc aacatggcca tcgaagccgg cgcgaaaaac ggcatctttc
    21301 cgccggacga aatcaccggg gaatacgtca aaccgcgcgc caaaaggcct tacacatttt
    21361 acacttccga tccggacgcc gtttacgcgc agacgattga aatcgacgcc tcccaactgg
    21421 agcttcaggt cgcctttccg catctgcctt ccaacgtcag gggaatcagc caggcaggcg
    21481 gcgtaaaaat tgaccaatcc ctgatcggga gctgcaccaa cggccggatt gaagatctgc
    21541 gcgttgccgc cggaatttta aaaggccgca aggccgcccc ccatgtccgc ctgatcgtgg
    21601 ttccggccac accgctcatt tatcggcagg ccctccggga agggctgctg gaaatcttca
    21661 tggacgcggg agcggtgatc agtccgccgt cgtgcggcgc gtgcctgggg ggccacctgg
    21721 gcatcctggc cgagggcgaa aaatccgtcg ccacgaccaa ccgcaatttt gtggggcgca
    21781 tgggccatcc caaaagcgaa gtctatctgg ccggccccgc cgtggccgcg gcatccgccg
    21841 ttctgggccg catcgcttca ccggaagagc tataaatcca aagggaacaa gaccatgatt
    21901 ttggaaggaa gagtctggaa attcggggac aacatcgaca ccgacgcgat catccccgcg
    21961 cgccatctca acacgtcgga tccggcccaa ctggcccggc actgtatgga agacgcagac
    22021 cccgccttca tcacccggat gaagccgggc gacatcatcg tcggcggaga aaacttcggc
    22081 tgcggatcat cccgggaaca cgcgcccatc gccatcaagg cggccggaat ttcctgcgtc
    22141 gtggcgaaaa gcttcgcgcg cattttctac cgcaacgcct ttaacatggg actgccgatc
    22201 ttcgaatccc gcgatcttgc cgacgcgatt gccgaaggcg cccggatttc gctcgacagc
    22261 gcgcagggtt cgattacggt ggaaggcctg gaccggaaat tttcaatcag cccgatcccg
    22321 ccctttatgg aacagctcat cgccgacggc ggcctgatga agcacatcgc cggaaaatga
    22381 gaaaagcatg acaaaacaaa atctgaagat cgccgttttc gccggagatg gaatcggtcc
    22441 ggaaatcatc cgggaagccc tgaaagtcct gaggctcgtc gccgaccgcc gcggcatctc
    22501 cctgacgctg acggagggac tgatcggcgg aagcgcctat gacgcgtgcg gcgccccctt
    22561 tcccgacgaa agcctgatgc ttgccctgca aagcgacgcc gtgctgctgg gcgcggtggg
    22621 cggaccgaaa tgggaaggcc tggactacag cgtccggccc gagcgcgcgc ttctgggcct
    22681 gcgcgaaaag ctggggcttt acgccaacct gcggcccgta ttggtctttg acgaactggc
    22741 ggacgcctcc cccctcaaaa agaatctggt gcagggcatc gacattctga ttttgcggga
    22801 gctgaccggc gacatctatt tcggccgtcc gcgcggcgtg accaaagacc aaagcggccg
    22861 gcgcaccggc gtcaacacca tgatctacac ggaagacgaa atccgccgca tcgcactgcg
    22921 cgcgtttgaa ctggcgcgcg gccgaaggaa aaaactcctg tcggtggaca aggccaatgt
    22981 tctggaaacc acggaactgt ggcgtcaggt cgtcacggag accggccgcg gctatcccga
    23041 cgtggcgctt tcgcacatgt atgtggacaa ctgcgccatg cagctcatcc gcaatccttc
    23101 gcagtttgac gtaatcgtga cctccaattt gttcggcgac attctgagcg acgaagccgc
    23161 catgctgacc ggctccatcg gcatgctgcc gtccgccagc ctgggcgacg ggaaggctct
    23221 gtacgagccg atccacggct ccgcgccgga catcgcggga aagaacatcg ccaacccgct
    23281 ggcaaccatt ctgtccgtcg ccatgatgct gcggcattcg ttggacagac cggcagaggc
    23341 ggcgctgatt gaacaagcgg taaaaagcgt cctgcgggac ggccggcgca ctccggatat
    23401 tgccccggcg ggcggaaggg cggacaccac ggaggaaatg ggcgacgccg tcgtccggaa
    23461 actttctacc ctcctgcatt catagccctg caaggcatac ggcggacaag gacgatcaac
    23521 atgccaaaca ccgtcacgat tctggatcat atcatcatcg ccccgcccgt ggcgaagatt
    23581 tattcccggc tcggctttaa aaaaaagatt acatctcttt cgtccgatca tcggcaggaa
    23641 acggaccggt atatggaaga agcggcttca atcattcgcc tgcagggttc cttccttcgg
    23701 ctttccatcc gcaaaaatga cggcctgacc gttgccctgg aaggacggac aaccttccgc
    23761 agcgagaagc tctgcgcgtt tctgggcaaa agccgggacg cggtgctgat gggagcaacg
    23821 gccggcaaac ccatcatgga cgccatcggt gaaaaaaccg ctcagggaaa cttaaccgcc
    23881 gccgtcgttt atgatgcgac agccagtgaa atgaccgacg cggcgctcga ctggatcatg
    23941 aattatgtca acggccggct ccggcgcgaa ggcaggcgcc tgctgccgcg ccgcttttcc
    24001 gcgggctacg cggacttttc cctggacaac cagaaagcca tgcacaaaaa actgcagatg
    24061 gaaaaaatcg gcgtctccat tacgccggac tgcattcttc atccggaaaa gtccgtcacc
    24121 gccatcggcg ccatcccctg aaatcccgga ggcgatcggc gcagcgccgc aagctccgga
    24181 gatccgcttc aggatttgcg ggcggaagaa tgctttcccc gcgggatttt cgaccggcgc
    24241 gctccgattc tctttttgta ctcgctgata aattccttgg acgacagata cttgtccgca
    24301 aacgacacga tgtaggattc cttgtatttg ggcggcacaa gggtcagcgg ccacatatgc
    24361 ttgcggataa tatcctcttc aatgtcgttg agcgagaagt attttctggc gttggccacg
    24421 gcgattttcg gatgttccag gccgtggaat ttttcccggg gcaaatccgg cgcgtcgtga
    24481 ttgcgccagt catagagaaa aaagtcgtgc agaagcgcgc cccgggccgt ggaacggtaa
    24541 tcaagcccga gaaacttgct gatccggtaa gacaggtagg ccacatcgtg gacatggcga
    24601 taaatggagg agttgtgatg gaagtaatcc ttgagcttcc ggaattcctc atgccgcaga
    24661 atatcgctca cggtctcgta atattccgct tcgaaaggct tccggccgtc gagctcctgg
    24721 aagatttttt ccaagacggg cttcagaaac gaaccgacct taatccggat gcgccggttg
    24781 atgttctggt tgacgtactt gttcaagtcg gggaaggctt cccgcaggcg ctggaaagac
    24841 ttcaggatat cctcgatttc gaggctgctt aagttgaagt attcctcgta cagataggca
    24901 acgcccttgc ggaaggcggc cagcgaaatc acggagaagg tgtagtcaat ccagaaatac
    24961 accaggaaga cgatggcggc aatctgcacc aggcccgcag gcagcatggc cgcccgccga
    25021 atggcttcgg ggtgaacaaa cagggcaaat cccgtcgcca gaagcgtcca gagcagggaa
    25081 aagctcaggc agacatagcc gtggagattg aaccgttttt ccgagtagtc ccacaacttc
    25141 agtttgaaga tcttttcgga cagatagccg gctgaatatt ccagaagggt gatcaccagg
    25201 ccaaaggcgg caaaacgaac cgccatgggc caatcctgca ggacgcgctc aagggcaagg
    25261 acgagaaagg cgccgatgcc gtaaatgggg aggaagggac cgaacaaaaa ccccgcattg
    25321 acgaatttcc gctgggtaac ggagcggtag atcacctccg ccacccatcc ggcaaagcag
    25381 tacgcggcaa acagaacaat gtaataaaaa aaattcagat ggtcatccaa catgggtttc
    25441 ccccgtcctt ttgtgttgcc cgatgctaca caattgcccc ctttttgtga agatattttg
    25501 cggccatctg tggtatgaaa cggaacgttg aaatatcacc gcctgccggc cacggcaaaa
    25561 agcgggaagg aacctgtatg atcaaagact gttttatcgc ggcgctcaag ggcatgggca
    25621 tgggcgcggc caacgtcatt cccggcgttt ccggcggcac gatcgccctg atcacgggca
    25681 tttttcagcg gcttatcgac gccctgaaat cgttcgatct tcaggcgttc aaactatttt
    25741 ttaggggccg gtggcgggcc tttgcccggc gcaccgactt cttttttctg ctggccgtct
    25801 ttgcgggtgc ggctgcgagc atcctgtcgc tggcccggct gttccggttt ctgttcgacc
    25861 attaccccgt cttcatctgg tcgtattttt tcggactcat tcttgcttcc gtcttttttg
    25921 tcggaaagac catcggccgc cggacgcctg ccgtggtcct ggtgtttatt ctgggagccg
    25981 ccatagccgc caccatatcg cttttgcgcc cggcggcgca aaacgattcc ttctggtatc
    26041 ttgtgatctg cggcgtcgtg gcgatctgca gcatgattct gccgggcatc tccggctcct
    26101 tcgtgctgat cctgatgggc aactatgaac tgatcgtcat cgacgcggtc aacgcgctgg
    26161 actggcgcat cctgcttccg gtggccctgg gcgccgccgt cggactggtg gccttttccc
    26221 atttcctgtc ctggattttc cgcaaattcc gcgccgaaac aatctccgcg ctgaccggct
    26281 tcattttggg atccctgatc accctctggc cctggaagac ggccgtctgg cgactgaacg
    26341 cggacggcgc ccccgtcgtg cgggacggaa aaaaactggc ggagggctat acctattctc
    26401 tgccgccgcc cgatgagcaa ttttggtggg cgctggctgt catggcggcg ggcgtcctca
    26461 cgatatgggc gacggagaag ctggccgttc gcaacgatcc cccgtaacaa gtctgcaaaa
    26521 cgcacgcggc tgcggcaggg aggcatcatg gaaaagatca aactgggcat cagcacctgt
    26581 cttctgggag aaaacgtccg ctatgacgga gggcacaagc tcgaccgctt cctgcgcgac
    26641 acgctgggac ggtatgtgga ttatgcgccg gtctgtccgg aaaccgaatg cggcatgggc
    26701 attccgcgcg aagccatgcg gctcgaaggg aacccggacg cgccccggct cgtcacccgc
    26761 agcacccgga tcgacaaaac ggaaatgatg gtttcctggg cgaaaaagcg cattgcccgg
    26821 cttgcgtccg aggatctctg cggttttatt ttcaaaagcg actctccgtc gagcggaatg
    26881 gaaagagtca aggtctatga cgaaaaaggc atgcccgcaa aaacgggtgt gggcatcttt
    26941 gcgcggatgt tcatggacgc ttttcccctg cttccggtgg aagaggaagg ccgccttcac
    27001 gatccggggc ttcgtgaaaa tttcattgaa cggattttcg cgctgagacg ctggcgtgag
    27061 gttcgcgggg gcaaatcccc ccggggcgct ctggtggatt ttcacacccg ccacaaactg
    27121 ctcctgctgt cgcacagcac aaaactctat cgggagatgg gaaagctggt cgccgaacag
    27181 aagcgccact cccttcagga tctttggtca aggtacgaag ccctgctcat ggaagcgctg
    27241 aagctgaaaa cttcgccgaa aaaacatgtc aatgtgctga tgcacatgat gggctacttc
    27301 cgggagaaac tctccaccga tgaaaaaaag gagctgctgg aaattttcga gcacaccgcg
    27361 cagggctttg tccccctgat cgttcccctg acgctgatcg gccactatgt gcgcaaatac
    27421 aacgagccgt atctgaaaga gcaggtttat ctgaatccgc atccgctgga gctgcagttg
    27481 cgaaaccatg tgtaaaaaca aattggccta cgcggcggcg gccgctgcgc gcggatagcc
    27541 gatgaactcg taaaaagtaa ccgaacagtc accccggcga ataccctaac cacctgacca
    27601 cctaaaggtg gcgcgagg
//
LOCUS       DAOSRM010000017        27205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1960_length_27205_cov_115.713554, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000017 DAOSRM010000000
VERSION     DAOSRM010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 27205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27205
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1960_length_27205_cov_115.713554"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..854
                     /gene="trpA"
                     /locus_tag="PLA58_03115"
     CDS             60..854
                     /gene="trpA"
                     /locus_tag="PLA58_03115"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOR61818.1"
                     /translation="MGRIGKKFEELRANKEKALIVYLTAGDPSLAATRELVLGLEKSG
                     ADILEIGVPFSDPTADGPVIQAASQRALKAGTTLRGVLELVAEIRRTSQIPIVLFGYF
                     NPIFAYGAEKFAQAAKDAGVDGILVVDLPPEEAGELRIFTDAAGIDFIALVAPTTGRD
                     RLKTIVQDATGFLYYISITGVTGAAAPKVEDISREVGKIRKMTKLPIAVGFGISCADQ
                     ARAIARIADGVVIGSAVVRRIDENRANSDCIQTVCAYVREIKEALS"
     gene            1019..1747
                     /locus_tag="PLA58_03120"
     CDS             1019..1747
                     /locus_tag="PLA58_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOR61819.1"
                     /translation="MKLKIGEIAKRSGLRPSTIRYYVGEGLLPKPEKANEKMAYYDEA
                     CVDRLRAIQELKGKRYFPLYLIKNILRRMDEGLNLTEAETIENAVFRSDGRAENGLLS
                     QAAFLAATGLTEKELTQARRLGILIPFATEGDKPLYDTDDVRAGRDGIKKMADLGMKL
                     QELSFWVELGRQIVEKEMALRRKMVAGKSTRENIAITAELTRVGDFYRDYILRRLFQK
                     QVEQNIQSSLKTAKKRKTGNTGKK"
     gene            1758..2786
                     /locus_tag="PLA58_03125"
     CDS             1758..2786
                     /locus_tag="PLA58_03125"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOR61820.1"
                     /translation="MYNTQITRMFGVQYPIICGAMMWLCKPSLCAAVCNAGGIGNLTA
                     ANYETEDEFRAAIRETRRLTDKPFFIGITLLPSIRITAEHHKMYVKVCAEEKVAGIEF
                     SGTPLDKVAGMEAVELLKKAGVKLFHKVGAVRHALHAEKIGFDGVYAAGIEEGGHPLN
                     DDVTTMILTPRMVESVKIPVVTVGGIANGRTMAAALTLGAQGVMMASRFIATKECEVH
                     DNIKQELIRRQEFDTVIYGKSIGLQGRGLKSKVIEEVLAIEARNGGFEELIPLLAGQK
                     VKQAWETGDVDYAPLMVGQSIGLISDIPTCAELLERMASEAKEQLARAGKLCGNEAQQ
                     NSSYKEQL"
     gene            2783..3820
                     /locus_tag="PLA58_03130"
     CDS             2783..3820
                     /locus_tag="PLA58_03130"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOR61821.1"
                     /translation="MKTRLTEMVGIKYPIVLSGMSWISVPKMVAAVSNAGGLGILATG
                     PLNAAQTREAIREIRKLTDKPFGANASLMFPGAVENAKVLLQEKVPVINFALGKGDWI
                     VKEAHKYGGKVFATVVNLKHAKRAQDFGTDAVIATGQEAAAHGEDVTSLVLIPHLAEN
                     LKIPVIAAGGFADGRGIVAALALGAEGVAMGTRLMTTKESPLHENYKRMTQEKDVYDT
                     LFSKRFDGILCRVMKTDAAKKAVKTGLNWPAAFFNSRDIAKQMGIPYFKLFIGVLLSG
                     YKNARQLAYMANAFKAIRVATEDGDSKTGVLPVGQSQGLIHDVPTIAELFERIVKEAK
                     DAQARVNAALE"
     gene            3878..4336
                     /locus_tag="PLA58_03135"
     CDS             3878..4336
                     /locus_tag="PLA58_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOR61822.1"
                     /translation="MSIESVLQELKPHLGKEVFVSGWKQVTQEQIHQFADSTGDHQWI
                     HVDTEKAAGGPFGRTIAHGFLTLSHIPFFSYQAPLKIEGAKMSINYGLDKVRFLNPVP
                     SGSKIRDRIVLSGLEEKPGDRLLVKQTHTIEIEGQEKPACIAEALAMVFF"
     gene            complement(4427..5401)
                     /locus_tag="PLA58_03140"
     CDS             complement(4427..5401)
                     /locus_tag="PLA58_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR61823.1"
                     /translation="MADEIRKGLYRISVPLPNSPLKDLNSYVIKGKDRNLIIDTGFNR
                     SVCYEAMRKGLAELGIDLQQTDFMLTHMHADHTGLVTRLATQTSRIFFSRIDARVFDG
                     DHGWQSLVDFAERNGFPAEELQKALASHPGFKYSPQKMPVFTLLDDGDVIECGNYRLR
                     CIATPGHTQGHICLYEEDQKIFFSGDHVLFDITPHIESWAYETNSLADYMASLDKVSH
                     LAVDLVLPGHRNFQGDLKSRIDALKIHHRERAEEVLEVLGNKTLNAYEIAAGMTWDID
                     CETWEDFPIAQKWFATGEAIAHLRYLEGEGRISRDAGQKIVTFSAVRP"
     gene            complement(5466..7346)
                     /locus_tag="PLA58_03145"
     CDS             complement(5466..7346)
                     /locus_tag="PLA58_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOR61824.1"
                     /translation="MKHVMLVNAVHKEQMRMATVDEKGKLIEFNIQMAVREPITGNIY
                     QGKVLKVERGLQAAFVDYGGVKDGFLPLRDVSHEYLTEPENGSSGGRPVLKTGQKIIV
                     QVVREVIGNKGALLTSYISLPGRYLVLLPNKLCGGISRKIESEEDRQKIKTLMDQIQV
                     PEGMGFIVRTAGINRTKQEIQRDYQFLMRLWKEIIKKAENASEPSLLYQETDFGVRSL
                     RDYLTLEIDEIWVDEVETFRKMRAYLKAVAPRHLRILKQYKDKVPIFERYGLEEQIRV
                     IYQERVELRSGGYIIINPTEAMITIDVNSGRGSNKRNIEETAFRTNVEACEEIARQLR
                     LRDLGGLIVIDFIDMIDKKHIAEVEKAFKKALSMDRARIQLSRISKFGLMELSRQKKQ
                     STIQEISYTTCPYCAGSGLRPSLEYASLGAFRKIESEAVKGTYSELKVSLPHEIALYI
                     LNNKRSELLKLEATFGVCLIIEGKQDMAWDNFEIQQSVKERALEVSVMQGVPLLNLAE
                     KTEPDAEAGEKPDAEAGEAEEVPKKKGRRRSRHKKRKSAEASKGAVVTGDATPDASGP
                     EPLPGAVTPPVPSSQFSRFPVKPLKIISALPDEFYPVDLSGDLFAPETDPEETGGDKE
                     ED"
     gene            complement(7443..8048)
                     /locus_tag="PLA58_03150"
     CDS             complement(7443..8048)
                     /locus_tag="PLA58_03150"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOR61825.1"
                     /translation="MLSINSQNPQMRLIRKATDVLRDGGIIIYPTDTVYGLGCDLSNK
                     RGIEKIYEIKRRNKKRPLSFVCSDLKHISQYALVTDYAYKTMKRFLPGPYTFILEASR
                     LVPKIILPKRPTTGIRVPANEICLALIRELGQPIISTSVQTQDGEDLGDPAIINEYFG
                     RTVDLIIDGGTIVPEPSSVVSLVDDAIEVLRIGKGDVSAFM"
     gene            complement(8138..9715)
                     /gene="cimA"
                     /locus_tag="PLA58_03155"
     CDS             complement(8138..9715)
                     /gene="cimA"
                     /locus_tag="PLA58_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOR61826.1"
                     /translation="MTQNRILIYDTTLRDGTQGEQVTFSAEEKLRIARRLDDVHFHYI
                     EGGWPGSNPKDMRFFEMAKTVKFKNAKLTAFSSTRKPRVRPDHCPNLKALIRAETPAV
                     AIFGKAWDLHVADILKVTLEENLSMIRDSIACMKARGKEVLFDAEHFFDGYKNNPRYA
                     LQVVRTALDAGADRIIFCDTNGGAMPHEVSAVLGKASAVIPMEKAGIHAHNDCGLAVA
                     NSLAAVREGVRIVQGTVNGYGERSGNADLISVIANLQIKMKMRCLPVASIRQLTNLSL
                     YVSDVANIPPLMSRPFVGRSAFAHKGGVHVSAVTKNSAAYEHMPPELVGNSRRVLVSD
                     MAGKSNIAYKAKALGIDLDKKNALGKVVREVKLMEDKGYQFDAADGSLSVLMKKAVGQ
                     FVEPFTLECFSVVTSRTLDNPCLSQATIKISVGGEEELTAAEGNGPVNALDHALRKAL
                     TKFYPQIKEMHLVDFKVRTLEGSEGTAAGVRVLLDSTDGSELWSTTGVSENIIEASWQ
                     ALIDSIEYKLSKDRKKR"
     gene            10033..12027
                     /locus_tag="PLA58_03160"
     CDS             10033..12027
                     /locus_tag="PLA58_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOR61827.1"
                     /translation="MDGLKFFHQLIIILGFSIPVIYVFNKIKIPSIIGFLITGILIGP
                     FGLKLIDDIAGISLMADIGVAFLMFTIGIEIRLSRFLKHLSEILLTGGMQLLCTFIIG
                     LLAGLTMQLSLSQAVLIGFLLVHSSSALILKLLKDRDDESSPQGRISIGVILFQDIMV
                     VPMMLMIPFLAAGSGPDALMILWKLLKSVLIIVALLIAARYVIPFALERLVSMNMRDV
                     LVIVSVVITMGIAWITESLGLSLAIGAFLAGLALSDTDFTHQIISDISPFRDVFLSVF
                     FVSFGMILNLDFLKAHPGSIGLASLGIILIKFAVVVSLVALMKYPLRSALLCGVLLSQ
                     IGEFSFVLASQGYRSRIISDEMYQTFIGASVLTFIITPLLASLTYWLLNRKSTLHSDS
                     TGSLQGQRVSLFNHVIICGMGLNGRNLARVLKNTAISYVMIDLNFQKIKKAKNSGDAN
                     AIWGDASNVEILKRAGVERARVMVIAISDRFLTKSCLSNAKVLNPKLHVIVRTKYLAD
                     IEELLALGADDVIPEEFETSIQIFSRVLKIFHIPNSIVLAQGNIIRNKSYGILRDVRF
                     TQEAFDQIGQILAQGTIETFYIAPGNPTIGKSIREVNLKAETGATIINIIRKNRTITN
                     PPGDFVFEAEDQLILFGSHSAIDLGLKKLEGNSANGSPAA"
     gene            12054..12821
                     /locus_tag="PLA58_03165"
     CDS             12054..12821
                     /locus_tag="PLA58_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOR61828.1"
                     /translation="MDIETRKQNGILTIEFNRPDKKNSITAAMYRMMADAIQDGEEDR
                     AVRVLLFCGKPEIFTAGNDLEDFMNMKSDVADRPVAQFIRNLSLAAKPVIAAVSGMAI
                     GIGVTMLLHCDQVYAARGARFSMPFAKLGLCPEFASSLLLPQIVGYQRAAELLFFGEP
                     FTAEQAFDMGLVNKVLPAAELMSYADGRAAGLAALPPASLRVTKQLLKARHLDAVTEQ
                     IALENRHFSAMLREPAAKEAFAAFYEHRKPDYSKIFD"
     gene            complement(12818..13813)
                     /locus_tag="PLA58_03170"
     CDS             complement(12818..13813)
                     /locus_tag="PLA58_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOR61829.1"
                     /translation="MDKTVTEHELSQPVVQYASEDFIALRARSSVAEALECIRSSRTQ
                     SAILYFYVVDDEGRLVGVVPTRRLLTSSLTASVESIMTGSLVSIPDNANLLEALEFFI
                     LYKFLAFPVVDADRKILGVIDVNLFTQEMIDMEEREQVHSIFDTLGVSVAEMKDRSPF
                     SVFRRRFPWLLATIASGTVCALLVGFFEATLAQSLILAFFLTLVLGLGESVSTQTMAI
                     TVHFMHHRRARSGWYFEVLRRELSRTLLLALACGAIVGAIAWIWRGEAAAAFVIGGGI
                     FLSLLLAGLIGVSVPSLLHRFKLDLRVASGPLTLALADICTVVAYFSLATKILVP"
     gene            13963..14379
                     /locus_tag="PLA58_03175"
     CDS             13963..14379
                     /locus_tag="PLA58_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61830.1"
                     /translation="MRQDDQVSRYEVNRNVRIVITRHDADLARIEYSFMGSTVYLSGD
                     LVRPNGDFSAQEVENIARELAALPHVRSLQFDLNNWLVDTSGDSWQVNRVRKTAGAGG
                     AAGQTGVSDDTTLVIEREEGLQDVIDDIEKTLKSKD"
     gene            14465..14541
                     /locus_tag="PLA58_03180"
     tRNA            14465..14541
                     /locus_tag="PLA58_03180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14499..14501,aa:Arg,seq:acg)
     gene            complement(14652..15950)
                     /locus_tag="PLA58_03185"
     CDS             complement(14652..15950)
                     /locus_tag="PLA58_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR61831.1"
                     /translation="MTEKAKTAAFYGWVLMPVLCLVYSIPIGFALYGPPVIYRFMQKD
                     LSWQRGEISLGYTIIGLTLGLGAFIIPWLMDRFGPRRTLAIGASVTSGSVLLMAFAGQ
                     SYPVYLLLCFFTGLGISFGSVVPVQALVLLWFNVHRALVMGLVLGGGAIGGFIYPQII
                     SASIVAFGGNWRVGWCVIALACVIGAVVAMIAVRNRPEDLGQHPDGMVPSAVREAMSD
                     ARIGRFRTYRSPVNWNFKDALKTKSIWLAIIGMSFIWFLWQSVLTQTPAHLSDRGFSM
                     SDPVWFLRPAFIYGLILACSIVGRLSVSFLGERMESRLLIAIAGFSLIGGGVLFWFAS
                     RENLWAVYLYPLLVGFGFGASYVSYPLLMGNYFGTESFHKISAIANPITSVFPYSGAF
                     FAGWLYDVNGGYGTAVVIACGIALVGSVLMLLCRPPVKAG"
     gene            16451..16849
                     /locus_tag="PLA58_03190"
     CDS             16451..16849
                     /locus_tag="PLA58_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61832.1"
                     /translation="MKKIGALVVMIGCFMICGKALAGGSAPNFAGTWMGEYCTMSSKE
                     GLRCGKPMEIVIDEQQKGLVRGYIKIGDKAYPLSGTISENRTISYTDALASVGVLRLV
                     GGKVLQMKAVNRCVAGNAECAHSGTFRRKK"
     gene            16851..17300
                     /locus_tag="PLA58_03195"
     CDS             16851..17300
                     /locus_tag="PLA58_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61833.1"
                     /translation="MKKIVQFACLPLLAFFLFPAPLAASADWVEYLRDADGNVFSYHR
                     KLNVDKTNGKHIVQALGKENLSGRGREKTLQSLREKGLPAAEYEQMSRKMFTCEIDCG
                     KNLIRKASLIAYDTNGKVIFSGKTSSPEWKPILPDSTEDALRKAVCE"
     gene            17672..18082
                     /locus_tag="PLA58_03200"
     CDS             17672..18082
                     /locus_tag="PLA58_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOR61834.1"
                     /translation="MISYQDVIIFILSKAYQRVYGIFKSRLQPYGLTPMQGLVLHALY
                     EEEGLSAGELGKRLSLDSATLSGVMDRMAESGWILKNVKDDRRVLNIRLTDKALSFRD
                     KIINDTEELNQEILSMFSMEERLLLIRMLKDLRK"
     gene            18138..19637
                     /locus_tag="PLA58_03205"
     CDS             18138..19637
                     /locus_tag="PLA58_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR61835.1"
                     /translation="MFDRTGNTKIHKRNYPATDYERLFYPRKVAILGVSSGENSIGFG
                     AGILKSILAMGFEGKIFPVNPKGGQIAGLNIYKSVEDIPEDIDFAIVSVAARFVPEAL
                     EACRKKGAAGAEILSSGFSELGTPEGQALEKQIQEVAAKGIRVVGPNCFGIYCPRSAL
                     TLLPGPDLSRESGPVGFLAQSGGMSIDFAHAAKWMGVRFSKMVSFGNGADLREAELLH
                     HLAGDPETKVITMYIEGIKDGEAFFREMKAAASQKPVIIYKGGLSRAGARAVASHTAS
                     MGGSRVIWQSMIRQAGAIQVADSEEMAQVTMAFSMLPQKSFRNITVIGGGGAIGVTAC
                     DLAETHGIEIPSLSAELQERIGNHLPKPGSSATNPIDVANPFVGPQPLREILLAAAED
                     PRIDLQMIVSLLFHYQGLAQAMNLSVNTVTPFRELAQAVRDVVDRTGKPVIAVLPNVK
                     RGLESMDVTEMMAMARQEYVNCGIPVFDEMHDAIRAVARVNTYYGGRKK"
     gene            19634..20320
                     /locus_tag="PLA58_03210"
     CDS             19634..20320
                     /locus_tag="PLA58_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOR61836.1"
                     /translation="MKNDKANRIIEKALKQGRKTLSEYEAKQVLSAYDIPITKEVLAK
                     DKAGMESGLKKIGFPLVMKGCSADIAHKTEKGLIHVDIRTAAEAKKAFREILAGMEGY
                     EGGVLMQEMIKGRRELVMGLTRDPQFGPCVMFGLGGIFTEILHDVSFRRAPLEIRDTK
                     EMMQEIKGHKILDAVRGMEAADQKLLSEMLISVGRIGLDFDNVMEIDLNPVILSGSKP
                     VVVDALIVLK"
     gene            20524..20727
                     /locus_tag="PLA58_03215"
     CDS             20524..20727
                     /locus_tag="PLA58_03215"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOR61837.1"
                     /translation="MDCKKERNLDICGCTYEPCSRKGICCDCVAYHLKKRQLPGCFFP
                     KDAERTFDRSFEHFADLVAQKKI"
     gene            21040..22206
                     /locus_tag="PLA58_03220"
     CDS             21040..22206
                     /locus_tag="PLA58_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HOR61838.1"
                     /translation="MTTPPNQDCQADYQVSFKKGKPDLLVGLKGEFSLKNLSAFARDI
                     RQAISDPSIQGVTVDFSQVCYLDSAAALAFVQLEKEAAERKITCSLVNLSAEAKGIFS
                     VIHPDALTLRPFKSEYSREGLVSQIGQASLSIGRDFVNFITFIGDLLIALAYTLMHPG
                     TLRGKDVLFYVQRAGVDGLPIVAMIGALMGLIMAFMSYLQFKQFGANIYVPTLVSFAM
                     IKELGPIMTAILVAGRSGSAFAAEIGTMLVNEEVDALQTMGFDPVRFLAVPKVLAAVI
                     VVPILTVYADVAGILGGLIIGVTTLDLTFKTYISQSIKTIQVFDVVTSLIKAAVFAGL
                     ISAIGCQRGFQVRAGAQDVGKFTTSAVVAAIFLIVLADSIFAVMLYYVKRYGIF"
     gene            22229..23026
                     /locus_tag="PLA58_03225"
     CDS             22229..23026
                     /locus_tag="PLA58_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR61839.1"
                     /translation="MPEPTEKTPILSVKNLTQRFGDNIVFENVSFDVCRGEVLVIVGS
                     SGCGKSTLLKIMIGLQKPYAGQVLYQGTDIIRASDEALNDYRKNIGVLFQSGALFSSM
                     SIKENIALPLQEYTDLDAETIDLVIRMKLGMVNLAGYENHNPSELSGGMKKRAGIARA
                     MALDPRVLFLDELSAGLDPITAVELDDLIIKTNEALGTTMVIVTHELESIYKIAHRVL
                     MLDKEARGIIAEGRPLELKEQATDPRVRSFFLRQLPEHQDEQRFYAN"
     gene            23016..23948
                     /locus_tag="PLA58_03230"
     CDS             23016..23948
                     /locus_tag="PLA58_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOR61840.1"
                     /translation="MPTRSSKFLIGLFVIIGALICAGVIIWVGAAKFFTKGALYNVYF
                     DESVQGLQVDSAIKYRGVEIGKVQSIDVAPDYRLIEVTMKIDLEGDLQKQTIASLKTA
                     GITGIVFIELDRIKPGELADSPKLSFKPDYPVIPSHRSEISKLITDTNAIMQNIKKID
                     FEGISNQLKNTSQAIENFVEGKRITNMMAHLENTSANLDETIAKISKTVAEGKVDQAV
                     TETLGVLTDARRLVGQARDGISALNLREKSAQMDAILEDIDKKTKTITTGLQDTSENL
                     RVTSENLQRLSESLNREPSDLIFSKPAPPRKPME"
     gene            23964..24632
                     /locus_tag="PLA58_03235"
     CDS             23964..24632
                     /locus_tag="PLA58_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOR61841.1"
                     /translation="MEKTLQRPARLWKQRSIPLWIVIALSLLCLSGCAGSGKPQYDVQ
                     KYLLTYPSPSFEASEKLPVSVKVNRFSIAAAYNSTGMIFRDDAYGLDAFNYSRWAVNP
                     ADMIGDLLLADMRAGNLFQAVLSRYDLEEGRFILSGGIEEFYLRAEKKNKVARIVLSI
                     SLQDSAEKETGKRIMYQKTYTREMPLQDASPKGYCAAASQAMQAVSREILRDVYAAVK
                     TKAP"
     gene            24647..25036
                     /locus_tag="PLA58_03240"
     CDS             24647..25036
                     /locus_tag="PLA58_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOR61842.1"
                     /translation="MKEISVRSASRFEMIDITEAIRQATREEKIESGIVLVYTPHTTA
                     AVTINENADPDVPRDLLAALDRAVPLSANYRHSEGNSAAHVKSSLVGASEMVIVENGR
                     LVLGTWQSIFFCEFDGPRTRKVFIKLM"
     gene            complement(25312..25974)
                     /locus_tag="PLA58_03245"
     CDS             complement(25312..25974)
                     /locus_tag="PLA58_03245"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOR61843.1"
                     /translation="MRDLTLHEQFELEILSRMQSGRLLDALVFTGGTMLRLCHGLDRY
                     SVDLDFWAARAGMDWTTYAHKLGSHLSPHYQIVDAANKRFSLVVELKSAAYPRSLKIK
                     IRKEVRPVKTETSIAYSSNSTVQVFMKTVALDDMMKSKIEAFLTRGEIRDAYDMEFLL
                     KRGVPLEADGKIVKRLLAGLQKLGKKDYSVKLGSLLPPELRQYYRENGFRILTSKLAA
                     KT"
     gene            complement(25962..26543)
                     /locus_tag="PLA58_03250"
     CDS             complement(25962..26543)
                     /locus_tag="PLA58_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61844.1"
                     /translation="MIRLQELGKKEYFTLEDAARNAGITTASARVLCSRYTRKGLLLR
                     LKNNLYTTAWRWENMKRFDFFKIANVLQVPSYISLMTALAYHEVTTQAQQGFFESVGR
                     KRTVTYAVRESVFQYTKIAGRYYGGFVRSGGVFIATAEKALLDAAYLYSFGKYKFDKD
                     SLDLSKLEMNSLKSLLQPYPQKTKETVKRLCGI"
     gene            complement(26626..>27205)
                     /locus_tag="PLA58_03255"
     CDS             complement(26626..>27205)
                     /locus_tag="PLA58_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61845.1"
                     /translation="QIDAQAHRRHGKAVSNFNCLYYVFHDDDKDLSGSCFAKDGSLER
                     HSKRDELFLTANFSRRLYITKGLRRYPHPPLSHQGKWALNRHGVLLQAPTVLTVLTLS
                     TSVGPSRNVVSGGVTIEFSRIAPKLFTGFEAKEGYNLATPEKALLDTIYLRKRVPFPD
                     ELDFDAIDRKKLVRLAEPFPATVKKRLKAIFQ"
BASE COUNT         6277 a         7693 c         7419 g         5816 t
ORIGIN      
        1 cttgcttttc gactttttac gggaccctcc gttgtaaatg aaaaagaaac gaggagatta
       61 tgggacgaat tggaaaaaaa tttgaagagc tgcgcgccaa caaggaaaag gcgctgatcg
      121 tttatctgac ggcgggcgat ccgtcgcttg cggcgacgcg cgaactggtt ttgggcctgg
      181 aaaaatcggg ggcggatatt ctggaaatcg gcgtgccgtt ttccgatccc acggccgacg
      241 gcccggtcat tcaggccgca tcccagcggg cgctcaaagc cggaaccacg ctgcggggcg
      301 ttctggaact ggtggccgag attcgccgga catcccagat tcccatcgtt cttttcggct
      361 acttcaatcc gatttttgcc tatggcgcgg aaaagttcgc ccaagcggca aaagacgcgg
      421 gggtggacgg aatcctggtg gtggatctgc cgccggagga ggcgggtgaa ctgaggattt
      481 tcacggatgc ggccgggatc gatttcattg cgctggtcgc cccgaccacc ggccgcgacc
      541 ggttaaagac cattgtgcaa gacgccacgg gatttctgta ttacatttcc attaccggcg
      601 tcaccggcgc cgcggcgcca aaagttgaag atatttcccg cgaagtcggc aagatccgca
      661 aaatgaccaa actgccgatt gccgtgggct tcggcatctc ctgcgccgat caggcaaggg
      721 ccatcgcccg catcgccgac ggcgttgtca ttggcagcgc cgtcgtccgc cggatcgatg
      781 aaaaccgggc aaacagcgat tgtattcaaa ccgtttgcgc gtatgtcaga gaaatcaagg
      841 aagccctttc ctgaggtttc ttttcgcctg cggcatcaaa accttcttca gaaatcaccg
      901 gacaaaccat tgatctggct ggattcacag gatttttcat attttagaca atttgcttga
      961 cagccgtctt ccgtttcact ataacattaa acctcaactt taatgtttgg ttcggcgaat
     1021 gaaattgaaa atcggtgaaa tcgcgaaaag aagcggcctg cggccttcga ccatccgcta
     1081 ttatgtcggg gaggggcttt tgcccaagcc cgaaaaagcg aatgaaaaaa tggcttacta
     1141 cgacgaggcc tgtgtcgatc gcctgcgcgc gattcaggag ctgaagggaa agcggtactt
     1201 ccctctttac ctcatcaaaa atattctgcg ccggatggat gaaggcctga atctgaccga
     1261 agccgaaacg atcgaaaacg ccgtgtttag gtccgacggg cgcgcggaaa acggactgct
     1321 gagccaggcc gcgtttctgg ccgcaacggg acttacggaa aaggaattga cgcaggcccg
     1381 gcgtctgggc atcctgatcc cgtttgcgac agaaggggac aagcctctgt acgacacaga
     1441 cgatgtccgc gcgggaaggg acggaatcaa aaaaatggcc gatctcggga tgaagcttca
     1501 ggagctgtcc ttctgggtgg aactggggcg gcaaatcgtg gaaaaagaaa tggcgcttcg
     1561 ccgcaaaatg gttgccggaa aatcgacacg ggaaaacatc gccatcaccg ccgaactgac
     1621 gcgggtcggg gatttctacc gggactatat tctccggcgc cttttccaga agcaggtgga
     1681 gcaaaacatt cagagcagcc tgaagacggc taaaaaacga aaaaccggaa acaccgggaa
     1741 aaaataggag gactttcatg tacaatacgc aaatcaccag aatgttcggc gtccagtatc
     1801 ccattatctg cggggcgatg atgtggctgt gcaaaccgtc gctgtgcgcg gccgtgtgca
     1861 atgccggcgg catcggcaat ctgaccgcgg ccaactacga aacggaggac gaattccgcg
     1921 cggccatccg ggaaacccgc aggctgaccg acaagccttt cttcatcggc atcacgctgc
     1981 tgccgtcgat ccggatcacg gccgagcacc ataaaatgta cgtcaaagtg tgcgccgaag
     2041 aaaaggtggc cggcatcgaa ttttccggca cgccgctcga caaggtggcg ggcatggaag
     2101 ccgttgaact tttgaaaaaa gcgggcgtaa agctgtttca caaggtcggc gcggtccgcc
     2161 acgctctgca cgcggaaaaa atcggctttg acggcgttta cgcggcgggc atcgaggaag
     2221 gcggccatcc gctcaacgac gatgtcacca ccatgatcct cacgccgcgc atggtcgaat
     2281 cggtgaagat ccccgtcgtc acggtcggcg gcatcgccaa cggccggacg atggccgctg
     2341 cgcttacgct gggcgcccag ggcgtgatga tggccagccg cttcatcgcg acgaaggaat
     2401 gcgaagtgca cgacaacatc aagcaggagc tgatccgccg ccaggaattc gacacggtga
     2461 tttacggaaa atccatcggc ctgcagggcc gcgggttgaa atccaaagtc attgaagaag
     2521 tactggccat cgaagcccgc aacggcggct ttgaagagct gatccccctg ctcgccggac
     2581 agaaggtcaa gcaggcctgg gaaaccggcg acgtcgatta cgcgcctctg atggtcggtc
     2641 agtccatcgg actcatttcc gacatcccca cctgcgcgga actgctggaa agaatggcgt
     2701 ccgaagccaa agaacaactg gcccgcgccg gaaaactttg cggaaacgaa gcccaacaaa
     2761 attcatcata taaggagcaa ctatgaaaac aagattaaca gaaatggtcg gcatcaagta
     2821 tcccatcgtt ctgtccggca tgagctggat cagcgttccc aaaatggtgg ccgcggtatc
     2881 caacgccgga gggctgggca ttctggccac cggtccgctg aacgccgcgc agacccggga
     2941 agccatccgc gaaatccgga aactgacgga taaacccttc ggcgcgaacg catcgctgat
     3001 gtttccgggc gcggtggaaa acgccaaagt tctgctgcag gaaaaagtgc ccgtcatcaa
     3061 tttcgcgctg ggcaaaggcg actggattgt gaaggaagcg cacaaatacg gcggcaaggt
     3121 tttcgcgacc gtcgtcaatc tgaagcacgc caaacgcgcg caggatttcg gaacggacgc
     3181 ggtcatcgcc accggtcagg aagcggcggc gcacggagaa gacgtcacct cgctggtgct
     3241 gattccgcac ctggcggaaa atctgaaaat tcccgtaatt gccgcgggtg gttttgccga
     3301 cggccggggc atcgtggccg cgctcgcgct gggcgcggaa ggcgttgcca tgggaacgag
     3361 actgatgacg accaaggaaa gccctctgca tgaaaactat aaacggatga cacaggaaaa
     3421 agacgtttat gacacgctgt tttccaagcg ctttgacggc atcctgtgcc gggtcatgaa
     3481 aacggatgca gccaaaaaag ccgtcaaaac cggattgaac tggcccgccg cctttttcaa
     3541 ttcccgggac atcgcgaagc agatgggcat tccgtatttt aaacttttta tcggggttct
     3601 gctgtccggc tacaagaatg cccggcagct cgcctacatg gccaacgcgt tcaaagccat
     3661 tcgggtcgcg acggaagacg gagattcaaa aaccggcgtg cttccggtcg gccagtcgca
     3721 gggtctgatt catgacgtgc cgaccattgc cgaactcttt gagcgaatcg tgaaagaggc
     3781 aaaagacgcg caggccagag tcaacgcggc cctggaataa acggacgcaa tccgaaaacg
     3841 agatgggtca ttccatcaac gggaaagggg gaaggacatg tcaattgaat cggtactaca
     3901 agaattgaag ccgcacctcg ggaaggaagt ttttgtctcc ggctggaaac aggtgacgca
     3961 ggagcagatc catcagtttg ccgacagcac cggcgatcac cagtggattc atgtggacac
     4021 ggaaaaggcc gccggcggtc cgttcggccg gaccatcgcg cacggattcc tgacgctttc
     4081 acacattcct tttttcagct atcaggcgcc cttgaaaatc gaaggcgcga agatgtccat
     4141 caactacggc ctcgacaagg tgcgttttct caaccccgtt ccttcgggat cgaaaatccg
     4201 cgaccggatc gtcctttccg gactggaaga aaaaccgggc gaccgccttc tggtgaagca
     4261 gacgcacacg attgaaattg aggggcagga aaagccggcg tgcatcgcgg aagcgctggc
     4321 catggttttc ttctgaggtc atccggcagg gagcggcaga caaccgttcc ctgccctcgc
     4381 ctgacaagaa cccgcgcgca atcgtcagga tcgtgaacgg aacgaatcag ggacggacgg
     4441 cggaaaaggt cacgatcttc tgcccggcgt cgcgggagat ccggccttcg ccttccaggt
     4501 agcgaagatg cgcgatggct tccccggtgg caaaccactt ctgggcgatg ggaaaatcct
     4561 cccaggtctc gcagtcgatg tcccaggtca ttcccgcggc aatttcatag gcattcagcg
     4621 ttttgtttcc cagaacctcc agaacttcct ccgcccgttc gcggtgatgg attttcaatg
     4681 cgtcgattct gcttttcaga tccccctgaa aattccggtg tccgggcagg acaaggtcca
     4741 ccgccagatg agatacttta tccaggctgg ccatataatc ggccagcgaa ttcgtctcat
     4801 atgcccagga ttcaatatgg ggcgtaatgt caaacagaac gtggtcgccg gaaaagaaaa
     4861 ttttctgatc ctcctcatac agacagatat gcccctgcgt gtggcccggc gtggcgatgc
     4921 agcgcagacg atagttgccg cattcgatca cgtcgccgtc gtccagcagg gtaaagaccg
     4981 gcattttctg cggactgtat ttgaatcccg gatggctggc cagcgccttt tgcagctcct
     5041 cggcggggaa gccgttgcgc tcggcaaagt caacaagcga ttgccagccg tgatccccgt
     5101 caaaaacccg cgcatcaatc cggctgaaga agatcctgct ggtttgggtc gccagccgcg
     5161 tcaccagccc cgtatgatcg gcgtgcatgt gggtcagcat aaaatcggtc tgctgtagat
     5221 cgatccccag ttcagccagg cctttccgca tcgcttcgta gcagacggaa cggttgaaac
     5281 ccgtgtcgat gatcaggttg cggtctttgc ctttgatgac gtaagaattc aggtctttga
     5341 gcggactgtt cggcagcggc acggaaatcc gataaagtcc ttttcggatt tcatccgcca
     5401 tggacaagcc tccctgaatt atggaaggac agcgcagcgc ttctgaaaag ctttcggttc
     5461 ggcattcaat cttcctcttt gtctccaccc gtctcttccg gatccgtttc cggcgcaaaa
     5521 agatcgccgc tcaggtcgac gggatagaat tcatccggca gcgcgctgat aatcttcagg
     5581 ggtttgacgg gaaaccgcga aaactgcgag gagggaaccg gcggcgtcac cgcgccgggc
     5641 aaaggctccg gcccggaggc atcgggcgtt gcgtcgcccg tcacaacggc gcctttcgac
     5701 gcctccgccg attttctttt tttgtggcgg gaacgacggc ggcccttctt tttgggaacc
     5761 tcttcggctt cgccggcttc cgcatccggt ttttcaccgg cttccgcgtc cggctccgtc
     5821 ttttccgcca ggttcagcag aggcactccc tgcatgacgc tgacttccag ggccctctcc
     5881 ttcacagact gctgaatttc gaaattatcc caggccatgt cctgttttcc ttcgatgatc
     5941 aggcagacgc cgaaggtcgc ctccagcttc aggagttcgg accgtttgtt gttcagaata
     6001 tacagggcga tttcatgcgg cagactgacc ttcagctccg aatacgtgcc tttgaccgcc
     6061 tccgattcga ttttgcgaaa tgcccctaat gacgcatact ccagagaggg gcgcagaccg
     6121 ctgccggcgc agtacggaca ggtggtgtag ctgatttcct gaatcgtgga ctgctttttc
     6181 tgacgggaca gctccatcag gccgaacttg gaaatgcgcg aaagctggat tctggcgcga
     6241 tccatgctca gggccttctt gaaggccttc tccacctcgg caatgtgctt tttgtcgatc
     6301 atgtcgatga aatcgatgac gatcagaccg cccaggtcgc gcagccgaag ctggcgcgca
     6361 atctcttcgc acgcctccac gttggtccgg aaagcggttt cctcaatgtt tctcttgttc
     6421 gacccgcgcc cggaattgac gtcgatggtg atcatggctt ccgtgggatt gatgatgatg
     6481 tagccgccgg acctgagctc cacgcgctcc tgataaatca cccggatctg ttcttccagt
     6541 ccgtagcgtt caaaaatcgg aactttgtct ttgtattgct tcagaatccg caaatggcgg
     6601 ggggcgaccg ccttcagata cgcgcgcatc ttgcggaagg tctccacctc gtccacccag
     6661 atttcatcaa tttccagcgt caggtagtcg cgcagcgacc gcacgccgaa atccgtttcc
     6721 tgatacagca gggacggttc ggaggcgttt tcggccttct tgatgatttc cttccacagg
     6781 cgcatcagga actggtagtc gcgctggatt tcctgcttgg tccggttgat gccggccgtg
     6841 cggacgataa agcccatccc ctcggggacc tggatctgat ccatcagcgt cttgatcttc
     6901 tgacggtctt cttcgctttc gatcttccgg gagatgccgc cgcagagttt gttgggaagc
     6961 agcaccagat accttccggg cagggagata tacgacgtca gcagagcgcc cttgttgccg
     7021 atcacttccc ggacgacctg gacaatgatt ttctggccgg tcttgagaac cggccttccg
     7081 ccggaagagc cgttttccgg ttcggtcaga tattcgtggc tgacgtcccg cagggggaga
     7141 aagccgtcct tgacgccgcc gtaatccaca aaagccgcct gcaggcctcg ttcgaccttc
     7201 agcaccttgc cctggtaaat atttccggtg atcggctcgc gcaccgccat ttgaatattg
     7261 aattcaatca gcttgccttt ttcgtcaacc gtggccatgc gcatctgttc cttgtgcacg
     7321 gcgttaacga gcattacatg tttcataaga atccttttgg gccgcggcct gcgtccgcgc
     7381 cattccggaa ggaatcgatg tcccgccggc gcgcggacgg ctcggcgccg ccctgtatat
     7441 ttttacataa aggcgctcac gtcgcctttt ccaatgcgaa gaacttcaat ggcgtcatcc
     7501 accaggctga cgacgctgga cggctcggga acaatggtcc cgccgtcaat aatcagatcc
     7561 acagtgcgcc cgaagtattc gttgatgatc gccgggtcgc cgaggtcttc tccgtcctgc
     7621 gtctgaacgc tggtgctgat gatcggctgt ccgagctccc ggatcagggc aaggcaaatt
     7681 tcgtttgccg gaacgcgaat gccggtcgtc ggcctcttgg gcagaatgat tttcgggaca
     7741 agacgcgacg cttccaggat aaacgtgtag ggtccgggca aaaaccgctt catggtttta
     7801 taagcgtaat ccgtaaccag cgcgtactgg ctgatgtgct tcagatcgga acagacaaaa
     7861 ctcaaagggc gttttttgtt tcttcttttt atttcgtata tcttctcaat gcctcgtttg
     7921 ttcgataaat cgcagcccag gccgtacacc gtatccgtcg ggtagatgat aatgccgccg
     7981 tccctcaaaa catcggtcgc cttccggatg agacgcatct ggggattctg gctgttgatc
     8041 gataacaccg ttcgggccct cgcttcacgt cgggcttcca tatcagcaat tgcaggattt
     8101 agcaatggcc gtaaacagcc aaaaaatcag cgggtcgtca cctctttttc cggtctttgc
     8161 tgagcttgta ttcgatgctg tcgatcagcg cctgccagct cgcctcgatg atgttttcgg
     8221 aaacgcccgt ggtgctccag agctccgaac catccgtcga atccagcaaa acgcggacgc
     8281 ccgcggccgt gccctcggag ccttccagcg tgcgcacctt gaagtccacc aggtgcattt
     8341 ccttgatttg cggatagaat ttggtcagcg ccttgcgcag ggcgtggtcg agcgcgttga
     8401 ccggaccgtt tccttcggcg gccgtcagct cctcctcgcc gcccacggaa atcttgatcg
     8461 tggcctggga caggcaggga ttatccagcg tccgcgaggt caccacgctg aagcactcca
     8521 gcgtaaaggg ctccacaaac tggccgactg ccttcttcat caggaccgac agagacccgt
     8581 cggccgcgtc gaactgatac cccttgtcct ccatcagctt gacctcgcgc accaccttgc
     8641 ccagcgcgtt tttcttgtcc agatcgatgc ccagggcttt ggccttgtag gcaatgttgc
     8701 ttttgccggc catgtcggag accagaacgc gccggctgtt gccgaccagc tccggcggca
     8761 tgtgttcgta agccgccgaa tttttcgtca cggcgctgac atggacgcct cccttgtgcg
     8821 caaaagcgct ccgccccacg aaaggcctcg acatcagggg cggaatgttg gccacatcgc
     8881 tgacatacag ggaaagattc gtgagctgcc ggatcgaagc caccggcagg cagcgcattt
     8941 tcattttgat ctgcagattg gcaatcacgg agatcaaatc ggcgtttccg gaccgctcgc
     9001 cgtagccgtt gaccgttccc tgaacgattc tgacgccttc gcgcaccgcc gcaagagaat
     9061 tggcaacggc caggccgcag tcgttgtggg cgtgaatgcc ggccttctcc attggaataa
     9121 cggcggatgc tttgcccagg acggcgctca cttcatgcgg catggccccg ccgttcgtgt
     9181 cgcagaaaat gatccggtcc gcgcccgcgt cgagagcggt cctgaccacc tggagggcat
     9241 accgggggtt gtttttgtag ccgtcaaaaa aatgctccgc gtcaaaaagc acctctttgc
     9301 ccctggcctt catgcaggcg atggaatcgc ggatcatgga cagattttct tccagcgtca
     9361 ctttcagaat atcggcaacg tgaagatccc aggccttgcc gaagatcgca acggccgggg
     9421 tttcggcccg gatcagcgcc ttgaggttgg ggcaatgatc gggccggacg cgcggctttc
     9481 gggtcgagct gaatgcggtc aatttcgcat ttttaaactt caccgttttg gccatttcaa
     9541 aaaagcgcat gtccttgggg ttggaaccgg gccagccgcc ttcaatgtaa tgaaaatgga
     9601 cgtcatccag acgcctggcg atcctcagct tttcttcggc ggaaaaggtg acctgctccc
     9661 cctgcgtgcc gtcgcggagc gttgtatcgt aaatcaaaat cctgttttgc gtcatggttt
     9721 cctctctttc ccataaaaaa atccgctgcc cggttttccc ggcagcggat ttgaattcct
     9781 gttgagcgta aatttcagct actccacggc caggcctcct cgggggaagc ggataattat
     9841 tctgctaatg atgatggccc gcaaaacatt cataatccgc accattaaat aacgaatttt
     9901 gcagaataat aatttcccga gtgcgttttg tcaatcaaaa tcagcgctcc ggcgatgttt
     9961 ttttggaaaa tttttccgat cagtgataaa caggatctca aagcaaattc ccttcaacga
    10021 tcggaaaggc ccgtggacgg actgaagttt tttcaccaat tgatcatcat tttaggcttc
    10081 tccattccgg tcatctatgt atttaataaa attaagattc cttccatcat cggctttctc
    10141 atcaccggca ttctgatcgg gcctttcggt ctgaaattga tcgacgacat tgccggcatt
    10201 tcgctgatgg cggacattgg cgtcgctttt ctgatgttca ccatcggcat cgaaatccgg
    10261 ctgtcccgct tcctgaagca cctctccgaa attctcctca cgggcggtat gcagcttctt
    10321 tgcaccttca tcatcggcct gctcgccggg ctgaccatgc agctcagcct cagccaggcc
    10381 gttttgatcg gctttctgct ggtccacagc tcgtcggctc tgatcctgaa gctcctcaag
    10441 gaccgggacg acgaatcgtc gccgcagggg cgaatttcca tcggcgtcat tttatttcag
    10501 gacatcatgg ttgtgccgat gatgctgatg atcccctttc tggccgccgg cagcggtccg
    10561 gacgccctca tgattctctg gaaacttctc aagtccgtcc tgatcatcgt cgcccttctg
    10621 atcgcggcgc ggtacgtgat cccgtttgca ctggagcgcc tggtcagcat gaacatgcgt
    10681 gacgtgctgg tgatcgtgtc cgtggtcatc accatgggca tcgcctggat cacggaatcg
    10741 ctgggactgt cgctcgccat cggcgcgttt ctggccgggc tggctctctc cgacacggat
    10801 ttcacgcacc agatcatcag tgatatcagc cccttccgcg atgtcttttt gtccgtgttt
    10861 ttcgtatcct tcggcatgat tctcaatctg gattttctca aggctcatcc cggctccatc
    10921 gggcttgcct ctctggggat catcctcatc aagtttgccg tggttgtgag cctggtcgct
    10981 ctgatgaagt atcctttgcg cagcgcgctt ctctgcggcg ttctgctgtc ccagatcggt
    11041 gaattttcct ttgttctggc ctcgcagggc taccggagcc gcatcatttc cgatgaaatg
    11101 tatcaaacct tcatcggcgc ctctgtattg acttttatca tcacacccct tttggcctcg
    11161 ctgacctatt ggctgctcaa ccgcaaaagc accctgcatt ccgattcaac cggctctctt
    11221 cagggtcaac gcgtttccct gttcaatcac gtgattatct gcgggatggg gctcaacggc
    11281 agaaacctgg ccagggtgct gaaaaacaca gccatcagct acgtgatgat cgatctgaat
    11341 tttcagaaaa tcaaaaaggc gaaaaacagc ggggatgcaa acgcgatttg gggcgacgcg
    11401 tccaatgtgg aaattctgaa gcgcgcgggc gtcgagcgcg cgcgcgtcat ggtcatcgcc
    11461 atttccgacc gttttctcac gaaaagctgt ctgtccaacg ccaaagtcct caacccgaaa
    11521 ctgcatgtga ttgtccggac aaaatacctt gccgacatcg aagagctgct ggctctgggg
    11581 gccgacgacg tcatccccga ggaattcgag acgtccattc aaatcttcag ccgcgtcctc
    11641 aaaatctttc atattcccaa cagcatcgtc ctggcgcagg gcaacatcat ccggaacaaa
    11701 tcctacggca ttttgcgcga tgtgcgcttc acacaggagg ctttcgacca gatcggccag
    11761 attctggcgc agggaaccat cgaaaccttc tacatcgccc cgggcaatcc cacgatcggc
    11821 aagagcatcc gcgaggtcaa cctgaaggcg gaaacaggcg ccacgatcat caacatcatc
    11881 cgcaaaaacc ggaccatcac caatccgccc ggcgattttg tttttgaggc ggaagaccag
    11941 ttgatccttt tcggcagcca cagcgcgatc gacctggggc tgaagaagct ggagggaaac
    12001 tccgccaacg gatccccggc ggcataattt tttcaaaccc tgaaggagta aaaatggaca
    12061 tcgaaaccag aaaacaaaac ggaatcctga ccatcgaatt caaccggccg gacaagaaaa
    12121 actccattac ggccgccatg taccggatga tggcggacgc gattcaagac ggcgaagaag
    12181 accgggccgt gcgcgtcttg cttttttgcg gcaaaccgga gattttcacg gcgggcaacg
    12241 atctggaaga tttcatgaac atgaagagcg acgtcgcgga ccgccccgtg gcgcaattta
    12301 tccggaattt gagccttgcg gccaaaccgg tgatcgccgc ggtctcggga atggccatcg
    12361 gcatcggcgt tacgatgctt ttgcactgcg accaagttta cgccgcccgg ggcgccagat
    12421 tctccatgcc gtttgccaag ctgggactgt gcccggaatt tgcctccagc ctccttctgc
    12481 cgcagattgt cggctaccag cgcgccgcgg aactgctctt cttcggcgag ccgtttaccg
    12541 ccgaacaggc gttcgacatg gggcttgtca acaaagtgct gccggccgcg gagctgatgt
    12601 cttacgccga cggccgggcc gccggacttg ccgccctgcc gcccgcgtcc ctgcgcgtca
    12661 ccaagcagct tctcaaagcg cggcacctcg acgcggtgac cgaacagatc gccctggaaa
    12721 accggcattt cagcgccatg ctgcgggagc cggcggccaa agaagccttc gccgctttct
    12781 acgagcatcg caaaccggat tattcgaaga ttttcgatta aggcacgagg attttcgtcg
    12841 ccagggaaaa gtaagcgacg acggtgcaga tgtcggccag cgccagcgta agaggcccgg
    12901 aggccacccg caaatccagt ttgaaccggt gcagcaaaga aggaacgctg acgccgataa
    12961 gaccggccag aagaagcgac aggaaaatcc ccccgccgat cacaaaggcc gccgccgctt
    13021 cgccgcgcca gatccaggca atcgctccca cgatcgcgcc gcaggcaagg gccaaaagca
    13081 gcgtgcggga aagttccctg cgcagcacct cgaaatacca gcccgaccgc gccctgcggt
    13141 gatgcataaa gtgaacggtg atggccatcg tctgggtcga aacgctttcg cccagcccca
    13201 acacaagcgt caggaaaaaa gccagaatca ggctttgcgc cagcgtggcc tcgaaaaaac
    13261 cgaccagcag cgcgcagacc gtgccgctgg cgatcgtcgc cagaagccag ggaaagcgcc
    13321 tccggaaaac gctgaacggc gaccggtcct tcatttccgc aacgctgacg cccagcgtgt
    13381 cgaaaatgct gtgaacctgt tcgcgctcct ccatatcgat catttcctgc gtgaaaagat
    13441 tgacgtcaat gacccccaga attttccgat cggcatcgac cacgggaaag gccagaaatt
    13501 tatagagaat aaaaaactcc agcgcttcca gcaaattggc gttgtccggg atcgacacga
    13561 ggctgccggt catgatggat tccacggagg cggtgagtga agacgtcaaa agccggcggg
    13621 tcgggacgac accgaccagc cggccctcgt catccaccac gtagaaataa agaatcgcgc
    13681 tctgcgtccg ggacgaacgg atgcattcga gcgcttccgc cacggagctt cgcgcccgca
    13741 gggcgatgaa gtcctccgaa gcatattgga cgacaggttg gctcagttcg tgttcggtga
    13801 cggttttatc cattctcggt ttcagatctc caggcaagat gcttttttgt acacgaatat
    13861 cccttcaaaa tcaaatctga agtgtcgcat cttccggcgc tcattccgcc cttgacaacc
    13921 ggacattctt atgaggtatt gaaaggcagg tgagagtggc gtatgcgaca ggatgatcag
    13981 gtttcccggt atgaagtcaa ccgcaacgta aggatcgtca tcacccgaca cgatgcggat
    14041 ctggcccgaa tagagtactc cttcatgggc agtacggtct atctgagcgg ggatctggtc
    14101 cggcccaacg gagatttctc cgcgcaggaa gttgaaaaca tcgcccgcga gcttgccgcc
    14161 ctgccccatg tccggagcct gcagtttgac ctcaacaact ggctggtgga cacgtcgggc
    14221 gattcctggc aggtcaaccg ggtcaggaaa acggccggcg ccgggggagc ggccggacag
    14281 accggcgtct ccgacgacac gaccctcgtg atcgaaaggg aagaaggcct gcaggatgtc
    14341 atcgacgaca ttgaaaaaac attgaagagc aaagactgac tcccatccaa ccctggccga
    14401 acattttcat tttgcatcag acggataatg tgctataggc caccttcgtt cttttgaaaa
    14461 caacgcgccc atggctcagc tggatagagc gccagactac gaatctggat gcccctggtt
    14521 cgaatccagg tgggcgcgcc agtaaaatca aggggttgca gatgcctggc atttgcagcc
    14581 tctttatcgt tttgtaacac tcctgtaaac attttcacgt tcttttcctg attcggtccc
    14641 gcggcgaatc ttcagccggc cttgacgggc ggccggcaca gcagcatcag cacggagccg
    14701 accagcgcaa tgccgcaggc gatgacgaca gccgttccat agccgccgtt cacgtcatag
    14761 agccagccgg caaaaaacgc gccggagtac ggaaaaaccg acgtaatggg attggcgatg
    14821 gcgctgattt tatgaaaaga ctccgtcccg aaatagtttc ccattaaaag cggataagac
    14881 acatacgacg cgccgaaacc aaagccgacc agcagcgggt acaggtaaac cgcccacagg
    14941 ttctcccggg aggcaaacca gaagagaacg cccccgccga tcaggctaaa accggcgatg
    15001 gcgatcagca gacggctttc catgcgttcg cccagaaacg agacgctgag ccggcccacg
    15061 atgctgcagg ccaggatcag cccgtaaata aaggcgggcc ggagaaacca aaccggatcg
    15121 gacatggaaa acccccggtc gctcaaatga gccggggtct gggtcagcac ggactgccac
    15181 aaaaaccaga tgaacgacat tccgatgatg gccagccaga tgcttttggt tttcagggcg
    15241 tctttaaaat tccagtttac cggcgagcgg taggttctga accgcccgat gcgggcgtcg
    15301 gacatggctt cgcgaacggc cgaggggacc attccgtccg gatgctgtcc aaggtcttcc
    15361 ggccggttcc ggacggcaat catggccacg accgcaccga taacacaggc gagtgcgatc
    15421 acacaccagc caaccctcca gtttcctccg aaagccacaa tggaggcgct gatgatctgg
    15481 ggataaatga acccgccgat cgcgcctccg cccagcacca ggcccatgac cagcgcccgg
    15541 tgaacattaa accacagcag aaccagcgcc tgcacgggca cgaccgaccc gaaagaaatt
    15601 cccaagccgg taaaaaagca taaaagcaaa tagacaggat aggattgccc ggcaaatgcc
    15661 atcagcaaaa ccgatcccga cgtcacagac gcgccgatgg ccagcgtcct gcggggccca
    15721 aagcggtcca tcagccacgg aatgatgaat gcgcccaggc cgagcgtcaa tccgatgatg
    15781 gtatagccaa gactgatctc tccgcgctgc cacgacaggt ctttctgcat gaaccggtaa
    15841 atgacgggcg gcccgtacaa cgcaaaaccg atcgggatcg aatacacgag gcacagaaca
    15901 ggcatcaaaa cccatccgta gaacgccgct gttttcgctt tttcggtcat gacgatgcgt
    15961 tcccctcccc cgaacatccg gttcctgacg tcacacagag agctttgaaa aatgtcattt
    16021 cgaggagcgt gagcgacgag aaatcttaag gatggccgtt tgttcaagat ttctcccttc
    16081 ggtcgaaatg acaaagaaga agtcgaaatg acagaaatgg ccgttattca aagctctccc
    16141 acacttgctt cagccttttc gaaatgtcgc gcttccccgc cgcattcctt gtcgcgcatc
    16201 atacccgact cttccgcctg gggccaccct ttttcaagga tccatttttt cgacttttta
    16261 cgaggccgtc cattttggag aatcggacga tctcctccgg cacaaattcc ccggaggaaa
    16321 aatatcctgt tgatcacagg cgcatttttc agttataaga gaaaagtgga aaggcccgca
    16381 gacctgtaaa gaaaacgaaa cggaaccgcc atttggggat tgcataaatt tgtttcagaa
    16441 aggagatggg atgaaaaaga tcggcgcact cgttgttatg atcggatgtt tcatgatttg
    16501 cggaaaagct ttggccggag gcagcgctcc gaattttgca ggaacctgga tgggggagta
    16561 ttgcacgatg agttccaaag aaggtctccg ctgcgggaaa ccgatggaga ttgtcatcga
    16621 tgaacagcag aagggtcttg tgcgggggta tatcaagatc ggggataagg cgtatccatt
    16681 gtccggcacg atcagcgaga acaggaccat ttcttatacc gacgcgctgg cttctgtcgg
    16741 cgttttgcgg ctggttggtg gaaaagtcct gcagatgaag gccgtcaacc gctgcgtggc
    16801 cggcaatgcc gaatgcgccc acagcggcac gttccggaga aaaaaatagc atgaaaaaga
    16861 tcgttcaatt cgcctgcctg ccgcttctgg ctttttttct cttcccggcg cccctggccg
    16921 cctcggcgga ctgggtggaa taccttcggg acgcggacgg aaacgttttt tcctatcaca
    16981 ggaagctcaa tgttgacaaa acgaacggaa aacacatcgt ccaggcattg ggaaaagaaa
    17041 acctttccgg tcggggcagg gaaaaaaccc ttcaatccct gagagaaaag gggttgcccg
    17101 ccgcggaata cgaacagatg tccaggaaaa tgtttacctg tgaaatcgac tgcgggaaaa
    17161 atttgatccg aaaagcttcg ttaatcgctt atgacacgaa cggcaaagtg attttttctg
    17221 gaaaaacatc ctctccggaa tggaagccca tccttcctga ttctacagaa gacgccctcc
    17281 gaaaagccgt ctgcgaatga atccccttcg ccccccggaa tcttcagagc cggaacatcg
    17341 gaagcggaaa gggtcaatca gttttgatga actcgtaaaa agcaattgaa accgtcactc
    17401 cggcgaatac cctaaccacc tgaaggtggc gcgagggcac aagcgccgga gtccaggcgt
    17461 cgtccccgcg gaggcagggg aaccagttcc tttattccct taccggatac cgtcccccga
    17521 cttctcgagg gcaggcttcg tcggtacgac gaaaagggtg cgaaaatgac ttgttacgag
    17581 gtcgtcagct tttgcaccag gcaacttttt tcttgacttg cctctgccgt tcatttatct
    17641 agcatacaaa ataatcagac cggcgggttc catgatcagc tatcaggatg ttattatttt
    17701 cattctcagc aaagcctacc agcgggttta cggaatattc aagtcccgct tgcagcctta
    17761 cggtctgacg cctatgcagg gccttgtctt gcacgctctc tatgaggaag aagggctttc
    17821 cgcaggcgaa ctgggaaaga ggttgtctct ggacagcgcc acgctgtccg gcgttatgga
    17881 ccgaatggcg gaaagcggct ggatcctcaa gaacgtgaaa gacgaccggc gggttttaaa
    17941 catccggctg acggacaagg cgctgtcgtt tcgtgacaaa attattaatg ataccgaaga
    18001 gttaaaccag gaaatccttt ccatgttcag tatggaagaa agactgcttc tgattcggat
    18061 gctgaaagat cttcgaaagt gacgattttg cttcatattt agcatgcaag ataaattttt
    18121 cgtcgggagg gtcaacaatg tttgacagga cgggcaacac taagatccac aaaagaaact
    18181 atccggccac ggactatgaa aggctatttt atccgcgcaa ggtggcgatc ctgggagttt
    18241 cttcgggaga aaacagcatc ggcttcgggg ccggcattct aaaatccatt ctcgccatgg
    18301 gatttgaagg gaaaatattc cccgtcaatc ccaaaggcgg ccaaatcgcc gggctgaaca
    18361 tttacaaaag cgtggaggat attccggagg acatcgactt tgccatcgtg tccgtagcgg
    18421 cccggttcgt tcccgaagcg ctggaagcct gccggaaaaa gggcgccgcc ggcgcggaaa
    18481 ttctctcctc cggcttcagc gaactgggaa ccccggaagg acaggctctg gaaaaacaga
    18541 ttcaggaagt ggccgcgaag ggcatccgcg tggtcggtcc caactgcttc ggcatttact
    18601 gccccagaag cgccctgacg ctgctgccgg gaccggacct ttcccgcgaa agcggacccg
    18661 tcggctttct ggcgcaaagc ggaggcatgt cgatcgattt tgcgcacgcg gcaaaatgga
    18721 tgggggtccg gttcagcaag atggtgagct tcggcaacgg ggcggacctg cgcgaagcgg
    18781 aactgctcca ccacctggcc ggcgacccgg agaccaaagt gatcaccatg tatattgaag
    18841 gcataaagga cggcgaggcc tttttccgcg aaatgaaggc cgcggcaagt caaaaaccgg
    18901 tgattattta caaaggggga ttatcccgcg ccggcgcccg ggcggtcgcc agccacaccg
    18961 cgtccatggg cggaagccgc gtcatctggc agtcgatgat caggcaggcg ggcgccattc
    19021 aggtggccga ctcggaggaa atggcgcagg tcaccatggc cttctccatg ctgccgcaaa
    19081 agtccttcag aaacattacc gttatcggcg ggggcggcgc gatcggcgtt accgcctgcg
    19141 acctggcgga aacacacggc atcgaaattc cttcgctttc cgcggaactc caggagcgca
    19201 tcgggaatca tcttcccaaa cccggctcca gcgcgaccaa ccccatcgac gttgccaatc
    19261 cgtttgtcgg gccgcaaccc ctccgggaaa ttttgctggc cgcggcggaa gatccgcgaa
    19321 tcgatctgca gatgatcgtt tcccttcttt tccattacca aggcctggcc caggccatga
    19381 acctgtcggt caacaccgta acgccctttc gggagctggc ccaggcagtc cgcgacgtgg
    19441 tggaccggac cggcaagccg gtgattgcgg tgcttcccaa cgtgaaacgg ggattggaaa
    19501 gcatggacgt gaccgaaatg atggccatgg cccgacagga atatgtcaac tgcggcatcc
    19561 cggtttttga tgaaatgcat gacgccattc gggctgtcgc ccgcgtcaac acctattacg
    19621 gaggaagaaa aaaatgaaaa acgataaggc aaaccggatc atcgagaagg ccctgaagca
    19681 ggggcgcaaa acgctttcgg aatacgaggc caaacaggtc ctgtccgcgt acgacatccc
    19741 gattacaaaa gaagttctgg ccaaagacaa ggccggcatg gaaagcggcc tgaagaaaat
    19801 cggctttcct ctggtgatga agggctgctc cgcggacatt gcccacaaga ccgaaaaagg
    19861 gttgatccac gtcgatatcc gaacggccgc ggaagcgaaa aaagcgtttc gtgaaattct
    19921 ggccggcatg gaaggttacg aaggcggcgt actgatgcag gagatgatca aaggccggcg
    19981 cgaactggtc atgggactga cgcgcgatcc gcagttcggc ccctgcgtaa tgttcggcct
    20041 gggaggcatc ttcacggaaa tcctccacga tgtttccttc cgccgcgcgc cgctggaaat
    20101 ccgggacacg aaagaaatga tgcaggaaat caaagggcac aagattctgg acgccgtgcg
    20161 cggcatggaa gcggcggatc aaaagctgct ttccgaaatg ctcatcagcg tcggccgcat
    20221 cggtctggac tttgacaacg tcatggaaat cgacctcaac ccggtgattc tctccgggtc
    20281 aaagccggtg gtcgtggacg cgctgatcgt tttgaagtaa tcggtaattg gtggatggtg
    20341 aatggtggat ggtgaatggt agggaacggc ttgtgacctc gcgccacctt caggtggtta
    20401 ggttatcgcg accgttccct acaaggacgt gagggacagg ctataggcga tgggctatgg
    20461 gcgataggaa aaaactctta gctcgtcgct cattgctcgt tactttccga aggaatatct
    20521 gccttggact gcaaaaaaga aagaaacctg gacatctgcg gctgcaccta cgaaccctgc
    20581 agccgcaagg gaatctgctg cgactgcgtc gcctaccacc tgaaaaagag gcagttgccg
    20641 ggctgctttt tcccgaaaga cgccgaacga acctttgacc gctccttcga gcatttcgcg
    20701 gatctggtcg cccaaaagaa gatatgaacc ggcggcacaa cggcgcggcc cttgctctgt
    20761 cgcccggctg aaagaaatcc tttgcatcat gtctcttcgt caaaagcgaa cgaagtgaag
    20821 caatctcatc agcattttat atgtgaggag atcgccacgt cgcttcgctt ctcgcgatga
    20881 acaaagtgta aagatgcaaa gtcttcagtt cgctcaggcc gcctttggtt tttcatccca
    20941 cgattttcat tgacaagcac cggccaaacg gcttaaatgc ctctgcaaat acatcctttt
    21001 caatatcggc atgttgtatc cagacgagaa aagaatgcca tgacgactcc gccaaaccaa
    21061 gattgtcaag ccgattacca ggtttctttc aaaaagggaa aaccggatct tctcgttggc
    21121 ctgaaaggag agttcagcct gaaaaatctt tcggccttcg cccgcgatat ccgacaggcc
    21181 atttccgatc catcgattca gggtgtaacg gtcgattttt ctcaagtttg ctacctggac
    21241 agcgcggcgg cgctggcttt tgttcaactt gaaaaagagg cggcagaacg gaaaatcacc
    21301 tgcagcctgg tcaacctgag cgcggaggcc aaaggtattt tcagcgtgat ccaccccgac
    21361 gctctgaccc tgcggccttt caaatcggag tacagccgcg aaggactggt ttctcagatc
    21421 ggccaggcct cgttatcgat cggcagggac ttcgtgaatt tcatcacgtt tatcggcgat
    21481 ctgctgatcg ccctggccta caccctgatg catcccggaa cgcttcgggg caaggatgtt
    21541 cttttttatg tgcagcgcgc gggtgtggac ggcctgccga ttgtcgccat gatcggcgcc
    21601 ctgatgggcc tgatcatggc cttcatgtcc tatctgcagt tcaagcagtt cggcgcgaat
    21661 atttacgtcc ctacactggt gagctttgcc atgatcaagg aactggggcc gatcatgacg
    21721 gcgattctgg tcgcgggacg ctccggctcc gcctttgccg cggagatcgg caccatgctg
    21781 gtcaacgaag aagtggacgc gctgcagacc atgggatttg acccggtccg ttttctggcc
    21841 gttccgaagg ttctggccgc cgtcatcgtc gtgccgatcc tgaccgttta cgcagacgtc
    21901 gccggcattc tcggcggcct gatcatcggc gtcacgactc tcgatttgac gttcaaaacc
    21961 tatatttccc agtccatcaa aaccatccag gtgtttgatg tggtcaccag cctgatcaaa
    22021 gccgcggtct ttgccggact catttccgcc atcggctgtc agcggggatt tcaggtgcgc
    22081 gccggcgccc aggatgtcgg caagtttacg acctccgccg tggtggcggc cattttttta
    22141 atcgtgctgg cggattccat ttttgccgtc atgctgtact atgtcaaaag atacgggatt
    22201 ttctaggaag acggatataa ggagcgccat gccggaacca acggaaaaaa cgcccatcct
    22261 ttcggtcaaa aacctgacgc agcgcttcgg cgacaacatc gtctttgaaa acgtgagctt
    22321 cgacgtgtgc cggggggagg ttttggtcat cgtgggcagc agcggctgcg gcaaatcgac
    22381 gctgctcaaa atcatgatcg gcctgcagaa gccttacgcc ggccaggtcc tgtatcaggg
    22441 aaccgacatc atccgggcgt cggatgaggc cctcaacgat taccgtaaaa acatcggcgt
    22501 tctctttcag tccggcgcgc tgttcagctc catgtccatc aaggaaaaca tcgcgctgcc
    22561 gctgcaggaa tacaccgacc tcgatgccga aaccatcgac ctggtcattc ggatgaaact
    22621 gggcatggtc aacctggccg gctacgaaaa tcacaacccg tccgagctgt ccggcggcat
    22681 gaagaaaaga gcgggcatcg cccgcgcgat ggccctcgat ccgcgcgtgc ttttcctgga
    22741 cgaactgtcc gccggcctcg atccgatcac cgcggtggag ctcgacgacc tgatcatcaa
    22801 gacgaacgag gccctgggaa ccaccatggt gatcgtcacc cacgaactgg aatctattta
    22861 caaaatagcc catagagtgc taatgctcga caaagaagcc cgggggatca tcgcggaagg
    22921 ccggcccctg gaactaaagg aacaggcgac ggacccgcgg gtcagaagtt tcttcctgcg
    22981 gcagttgccc gaacatcagg acgaacagag gttttatgcc aactagaagc tctaaatttt
    23041 taatcggcct tttcgtcatc atcggcgcgc tgatctgcgc cggcgtgatc atctgggtcg
    23101 gcgcggccaa atttttcacc aagggcgccc tctacaacgt ctattttgac gagtccgtcc
    23161 agggattgca ggtggactcg gccatcaaat accggggagt cgaaatcggg aaagttcaga
    23221 gcattgacgt ggcgcccgac tatcgcctga tcgaagtcac catgaaaatc gacctggaag
    23281 gagatttgca gaagcagacc atcgcttcgc tcaaaaccgc cggcatcacc ggcattgtct
    23341 ttatcgaact cgaccggatc aaacccggcg aactggctga ttctcccaaa ctttccttca
    23401 agccggatta cccggtcatc ccgtcgcacc gttcggaaat cagcaagctg atcaccgaca
    23461 cgaacgcaat catgcaaaac attaaaaaga tcgacttcga gggcatttcc aaccagttga
    23521 aaaacacttc gcaggccatc gaaaattttg tggaaggcaa gcgcatcacg aacatgatgg
    23581 cccacctgga aaacacctcc gccaatctgg atgaaacgat cgccaaaatc agcaagaccg
    23641 tcgccgaagg caaggtggac caggccgtca cggaaaccct gggcgttttg accgacgccc
    23701 gcaggctggt cggtcaggcc agggatggaa tctccgccct gaacctgcgt gaaaaatcgg
    23761 cgcaaatgga cgctatcctg gaagacatcg acaaaaagac aaaaaccatc accaccggcc
    23821 ttcaggacac gtcggaaaat ctgcgggtca cgtcggaaaa tctgcagagg ctttccgaaa
    23881 gcctgaaccg ggagccgtcg gacctgattt tcagcaaacc ggcgccgccg cgaaagccca
    23941 tggaataaag aaaggaacga gcaatggaaa aaactttgca aaggccggcc cgcttgtgga
    24001 aacaaagatc catcccgctc tggatcgtca tcgctttgtc tctgttgtgt ctttccggat
    24061 gcgccggcag cggcaaaccg caatacgacg tgcaaaaata cctgctgacc tatccgtcac
    24121 cctcctttga ggcgtcggag aaacttcccg tgtccgtcaa ggtgaaccgg ttttccattg
    24181 ccgccgcgta caattcgacc ggcatgattt tccgcgacga cgcctacggc ctggacgcct
    24241 tcaattattc gcgctgggcc gtcaaccctg ccgacatgat cggcgatctc cttctggccg
    24301 atatgagagc cggcaatctt tttcaggcgg ttctgtcccg gtatgatctg gaagaagggc
    24361 ggtttatcct ctcgggcggc attgaagaat tttacctgcg ggcggagaag aaaaacaaag
    24421 tcgcacggat cgttctttcc atttcgcttc aggactccgc agagaaagaa accggcaaaa
    24481 gaataatgta ccaaaagacc tacacccggg aaatgcctct gcaggacgca tcgcccaaag
    24541 gatactgcgc cgccgcaagc caagccatgc aggccgtttc ccgcgaaatc ctccgcgacg
    24601 tttacgcggc cgttaaaacg aaggcgccat aaacagcggg ggttttatga aagaaatttc
    24661 cgtccggagc gcttcacgtt ttgaaatgat cgacatcacc gaagccatcc ggcaggcgac
    24721 gcgcgaagaa aagatcgaat caggcattgt tctcgtttac acgccccaca ccaccgccgc
    24781 cgtcacgatc aacgaaaacg cggatccgga cgtgccacgc gatcttctgg cggcgctcga
    24841 tcgcgccgtg cccctgtccg cgaactaccg gcattcggaa ggcaattccg ccgcgcacgt
    24901 caaatcgtcg ctggtcggcg cctccgaaat ggtcatcgtg gaaaacggcc gccttgttct
    24961 gggcacctgg caatccatct tcttctgcga attcgacggc ccccggacaa ggaaagtctt
    25021 tattaaatta atgtagggat cggtccccga acgatccggg tcggcaatct ctttgcgatg
    25081 tacagcctcc ggctgaaatt agcggagacc tttgaagaac ggttcaagag ttgtaacatt
    25141 gtcatcccgg cggaggccgg gatccagtgt tttcaaggac tatctggatt ccggatcaag
    25201 tccagaggaa tgacgggaaa aggacttatt caaaccaggt ctctagcggc aaggaataat
    25261 tccttttgct gtgccgctca agactgccgt ctttcgcgaa gcacgacccg ctcaggtctt
    25321 tgccgccagt tttgacgtaa gaatccgaaa cccgttttcc ctgtaatatt gacgcagttc
    25381 ggggggcaaa agcgagccga gcttgacgga ataatccttt ttaccgagct tttgcaatcc
    25441 cgccagcagc cgtttaacga ttttgccgtc cgcctccaaa ggcacgcctc ttttgagcag
    25501 aaattccatg tcgtaggcgt cgcggatttc cccgcgagtg agaaaagctt caatttttga
    25561 tttcatcatg tcgtccaacg cgacggtttt catgaatacc tgaaccgttg agttggaact
    25621 gtaagcaata ctggtttcgg tctttaccgg cctgacctct ttgcggattt taattttcag
    25681 cgagcgcggg tatgccgccg atttcaattc aaccacaagg ctgaatcgtt tatttgccgc
    25741 atccacaatt tgataatgcg gcgatagatg cgacccgagt ttatgggcgt atgtcgtcca
    25801 atccatgccg gccctggccg cccagaaatc caaatcaacc gaatagcgat cgagcccgtg
    25861 gcacaggcgc agcatggtcc cgccggtgaa aaccagcgca tccagaagcc gtccgctttg
    25921 catgcggctg agaatttcca gttcaaactg ttcgtgcaat gtcaaatccc gcacagccgt
    25981 ttcaccgttt ctttcgtttt ttgcggatac ggttgaagca ggcttttcag gctgttcatc
    26041 tccagcttgc tcaaatccaa agaatcttta tcaaatttgt atttgccgaa ggagtacaga
    26101 taggccgcat ccagaagcgc cttttccgcc gttgcgataa acacgccccc gcttcgaaca
    26161 aatccgccgt agtatcgccc ggcgattttt gtatattgaa aaacgctttc ccgaacggcg
    26221 tatgtcaccg tccgtttccg gccgacgctt tcaaagaagc cctgctgcgc ctgggtggtt
    26281 acttcatgat acgccagcgc cgtcatcagg gaaatgtagg atggaacctg cagaacattg
    26341 gcgattttaa aaaaatcgaa gcgcttcatg ttttcccagc gccaggcagt tgtgtacaag
    26401 ttattcttca agcgaagcag caaccctttt cgcgtatagc ggctgcaaag cacgcgggcg
    26461 gacgcggtcg ttatgccggc atttcgcgcc gcatcctcca gtgtaaaata ttcttttttg
    26521 ccgagttcct gcaatcggat catttattcg ccaggcacat ataattatca ttattgttaa
    26581 ttatatattc ctggtaatat tttgcaagcc atttcatgca ccccgttatt gaaaaattgc
    26641 tttcagccgc tttttaaccg tcgccgggaa cggttcggcc aaccgaacca gttttttgcg
    26701 gtcgatagcg tcaaaatcca gttcatcggg aaacggcacg cgttttcgta aataaatggt
    26761 gtcgagcagc gccttttccg gcgttgcaag attgtagcct tcttttgcct caaaacccgt
    26821 aaaaagcttc ggggcaattc gggaaaattc gatggtaacg ccgcccgaaa cgacgttgcg
    26881 cgacggcccc accgacgtcg aaagcgtcag cacggttaag accgtgggcg cctgcagcag
    26941 aacaccgtgg cgattgagcg cccatttccc ttgatgggag aggggtgggt gagggtaacg
    27001 tcgcaatccc ttcgtaatgt acagcctccg gctgaaatta gcggtaagga ataattcatc
    27061 ccttttgctg tgccgctcaa gactgccgtc tttcgcgaag cacgacccgc tcaggtcttt
    27121 gtcgtcatca tgaaaaacat agtaaaggca attaaaattg ctgacggcct tgccgtgacg
    27181 cctgtgagcc tgcgcatcaa tctgc
//
LOCUS       DAOSRM010000018        27202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1961_length_27202_cov_119.244336, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000018 DAOSRM010000000
VERSION     DAOSRM010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 27202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27202
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1961_length_27202_cov_119.244336"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2060)
                     /locus_tag="PLA58_03260"
     CDS             complement(<1..2060)
                     /locus_tag="PLA58_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOR61846.1"
                     /translation="MKYFDILVGYFRTSGFDKLHESFASIDKIRILVGLNVDRQAFEI
                     IDAFQTQSALDFESHKRTKEIFSSAVTEEMDCSPDSYETELGVRKFIEFLRSGKMEIK
                     AYPSGNLHAKVYISRFGEDDRDFGRVITGSSNFSEAGLIANREFNVELKNRADVEFAL
                     ARFEELWKDAVDLSHEYVDTIHDKTWLNDEITPYHLYLKFLYEHFREDINIDEDFETY
                     LPEGFMDLDYQKQAVIAAKKILDAYNGVFLADVVGLGKTFISAMLAQQLRGKILVICP
                     PVLKDYWEETFFEFGVRGYKVESLGKLDHVIKEGAAKFDFIFIDEAHRFRNEVTQGYE
                     KLHQICFGKKVILVSATPLNNTVEDIYSQLKLFQIPKKSTIPGVADLEKFFAGLKTRL
                     DRYAKTDPGYAEEIKDVSRDVREKVLKYVMVRRTRSEVVNFFKNDISKQGLSFPELGD
                     PQRLIYTFDARQERIFNATIELLKKFCYARYTPLLYLKKQLDPLELQSQRNIGGFMKG
                     ILVKRLESSFHAFGNTLKRFITSYEKFIAMIEQGKVYISKRVNVYDFLEEDNEEALLN
                     LVEQDRVQKYTADEFTDDFLTRLNDDLNLLKEIKRIWMGITGDPKIEQFITDLRGHAI
                     LKGKKLLVFTESKETGEYLYRRLEREFPGEAMFFCSTGGFYDGVEFGPAPARDLVKDH
                     FDPNR"
     gene            complement(2424..2849)
                     /locus_tag="PLA58_03265"
     CDS             complement(2424..2849)
                     /locus_tag="PLA58_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOR61847.1"
                     /translation="MKYLLDTCVISELVKKEPHPSVIRWMDTGDESKMYLSVLTLGEI
                     IKGINKLPNGDRREKLQSWVTNDLVQRFGSRLLEIDAEISMAWGTMLGEAERLGEKLP
                     VVDSLIAASANVHDLIVVTRNVKDMERCRTKVFNPWEAQ"
     gene            complement(2846..3121)
                     /locus_tag="PLA58_03270"
     CDS             complement(2846..3121)
                     /locus_tag="PLA58_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOR61848.1"
                     /translation="MTRTNTKKKDPSWQLQEAKSRLSQVVEHALREGPQTITLRGKPA
                     VVVVSFEEFQNITRPGTSLREFLRQSPLYGTDLDLERSKDVSREVHL"
     gene            complement(3460..4353)
                     /locus_tag="PLA58_03275"
     CDS             complement(3460..4353)
                     /locus_tag="PLA58_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61849.1"
                     /translation="MMRTYTLKYVELAEQHAEKMAKRWALDVQNNAKTPTYKNLNEQK
                     IIFQCVQFYRNFSKMFVHEKISEEVQKYFRSYAVDCYALGIPMAEMVYALILMRRHIW
                     LYAEFQMIFSSLINQQQALDTLNRTILLFDYASYDVTREYQELMKRDKLESIKLLDIL
                     ESNVVEIAANWAATVRKDRQTVYYHNIPKEKLMPQAIKFYSHLRTLLFDPERFEKGRE
                     FFRQYAETCRQQGIPLHEAIYALNVMRRQMWLHDEFQRTFVNALAHQQAVDSLMRIML
                     LMDYAAFDITHYYQERMPQEN"
     gene            complement(4368..5018)
                     /locus_tag="PLA58_03280"
     CDS             complement(4368..5018)
                     /locus_tag="PLA58_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOR61850.1"
                     /translation="MPKIELPPTKSSLRRLKEDLAFAYEGYDLLNQKREILAIEIVRK
                     IAEIRRIEAEFQQGLNQLYGAYRSAAVDMGSDVASRQSCSEVRSYFLRTDFTKLMGLR
                     MPVIRLRLKNPHPAFTLPQTTAAYDEARDQSRKTLLTLAEYATMTKSVMMLSRELKKV
                     QRRVNALEKIFIPQHEESKKYITDRIEEMEREEVFVKKLIRQRGQSGMTKESKPNP"
     gene            complement(5277..6668)
                     /locus_tag="PLA58_03285"
     CDS             complement(5277..6668)
                     /locus_tag="PLA58_03285"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOR61851.1"
                     /translation="MSAEIEYTNLSGIAGPLIFVDGIRGVGYGDIVEIRDASGELRTG
                     QTLEVDEQRAVVQVYEGTSGLSVKGTRVTFVGRALEIPVDRDMLGRVYDGLGRPIDGA
                     PQVITDRMMNVNGLPINPWSREYPRDFIQTGISAIDGMNTLIRGQKLPIFSGSGMPHN
                     RIAAQIARQARILTAEEEFTVIFAAMGVKFDVARFFIDSLEESGVLTHSAIFLSLADS
                     PSIERLVTPRTALTLAEYLAFEEGMHVLVILTDMTNYCEALRELSSSRGEIPSRKGYP
                     GYLYSDLASVYERAGRLKGGKGSITQVPILTMPSDDISHPVPDLTGYITEGQIVLERA
                     LFNKGIYPPIAGLPSLSRLMKDGIGEGRTRKDHGQVAAQLFASYAKVKRIRALAAIVG
                     EEELSDLDKVYLKFGTAFEEIFLRQDYEENRSIEQTLDIGWRMLSLLPRDELYRIARE
                     DIQKYYMPEAASA"
     gene            complement(6670..8442)
                     /locus_tag="PLA58_03290"
     CDS             complement(6670..8442)
                     /locus_tag="PLA58_03290"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOR61852.1"
                     /translation="MEKLLTGKVISVNGPIVKSVGLSGIRMYDIAEVGPDRLIGEVIR
                     LAEEIAIIQVYEDNTGLKPGDEVISYGRPLSVLLGPGLIASIYDGIQRPLVGISEACG
                     SYIRKGIKLSPLNAKAKWNFKPLVSSGAKVGEGALVGEVQESPLVLHRLLVPPGVSGT
                     LTRIAPAGSYTIEEPIYLIDTDAGAYEGKLAAYWPVRRPRPSKTRKKPFIPLVTGQRV
                     IDTFFPIARGGTAAIPGGFGTGKTMTQHALAKWCNADIIVYIGCGERGNEMTDVLTDF
                     PKLIDERNGRPLIERTVMIANTSNMPVPAREVSIYTGVTIAEYYRDMGYSVAVMADST
                     SRWAEALRELASRLGDMPAEEGFPPYLATRLAEFYERAGLVETLSGKDGDITIIGAVS
                     PPGGDFSEPVTQHTSRFVRCFWALDKTLADARHYPSISWIDSYTEYLSDIEDWWKNLD
                     PQWLTTRNEVMNILLEDHRLEQVVKLVGSDALPPAEQYILFCAESVKNAFLQQNSFDP
                     QDKFCSPEKQLRILASLLLLYKKGLEQVRAGVSVKEIAALEEASRLVRLKSEISNQDI
                     GKIDEYMNRLEQKLASLSAPSKGA"
     gene            complement(8518..9135)
                     /locus_tag="PLA58_03295"
     CDS             complement(8518..9135)
                     /locus_tag="PLA58_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61853.1"
                     /translation="MDNTSLENSIKRASEEMIAEIREKEARELGRLDEDYAAEIERFR
                     KTMEAETRSRMDQELSRLENRAVLERKKLKLTTVERFIQRIVEDVMRGIRSHPRYRQF
                     LMDAAAGAAAQIPENVEVRLAPDDGVYGNEIDEALRIAGDHRQIRIEADAAIGWGGCL
                     VVDSARGRIFNRTLERIYFRKALQIRRKVLQVLQDRTEGKESVTR"
     gene            complement(9201..9506)
                     /locus_tag="PLA58_03300"
     CDS             complement(9201..9506)
                     /locus_tag="PLA58_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOR61854.1"
                     /translation="MEKIYMIGDMDTVSAFRLAGVEGVVAEPSRAAAALERIVGKKDA
                     GVVLITGALASGLGERIAEINLGRPSPVVVEIPGMDDPEGFRGSVMAYVEEALGISL"
     gene            complement(9508..9840)
                     /locus_tag="PLA58_03305"
     CDS             complement(9508..9840)
                     /locus_tag="PLA58_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOR61855.1"
                     /translation="MTRSRQLAFLMGAIFLYALWSLFPAMGWASEASPVKALAEAIGL
                     AYIGAGVAVGFACLASGYAVAKIGSAAIGAVSEKPELMGRTLVFLGLAEGIAIYGLII
                     AIMILNKL"
     gene            complement(9842..11728)
                     /locus_tag="PLA58_03310"
     CDS             complement(9842..11728)
                     /locus_tag="PLA58_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOR61856.1"
                     /translation="MFVKSDIRKITIAFDKEHQRRVYLALGKAGLVHLARFHEKDSLA
                     DGGLQGEESLTREILSATAYTLAALHLEPDERGGEARLVNTAQDEAEASGVKRKLERA
                     LRLQTKIRDQREILARDIEYARALERMGIDAEMLKKPGILQTVFGTVEQTDWLVPSDE
                     RFMIVRTGRFVFGMALPAFAADMMQFLKENGFADQAKDVRTISSAALEKRADILRRRL
                     AALDAYVNRLKEEKGEFLRRMNADYRAYEEVLKAIRLSVISSRAMFLTGWVDAKEKAI
                     LMEIMKEVCGGGFVVSEQKDANAPVRLRNLWLFRPFELLVKTMGMPSSNELDPTPLAA
                     VTYVMMFGLMFGDLGQGIVLMLCGWLLKKFGQKKAREELAQAGGILLACGGSAAVCGL
                     LYGSVFSSEHLLPALWFHPTAQIMRLFFFTIMMGVVFILVGLCLNIINSFVNADYSEA
                     LLEKRGLAILVVYAAAVVFVLQYLKTGQPPALWALGIFMVLPLMIFSLRGLLGVLFLE
                     KAKPHSPSEYIVETIMEVVEIALGLFANTISFIRVGAFALSHAGLSIVTYTLAGMADP
                     SLKSVGAVIIIVCGNIFIIGFEGLICGIQSLRLEYYEFFSKFFKGDGVVFSPFTLPAK
                     ASEV"
     gene            complement(11737..12687)
                     /locus_tag="PLA58_03315"
     CDS             complement(11737..12687)
                     /locus_tag="PLA58_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOR61857.1"
                     /translation="MRNYADVPNIHTRIYAMKSRLFSLRDYILMIREPETLPGHVSGI
                     QDPIQAKEKLFEEQIAPIFPLVAAHEKYTPFFIAHLRQYEAHNAKILLATAAGHHSFR
                     QWYNIGPFAALDRGLLEERLSLDDVKTLLAGIYQDETFRAISGYRQLVVHLDDCTARN
                     LYRSAELLVGSAAAEFREMMLKRLAVMTLIWSYRLGVHHRLPQEKVRFYMDKFQALYG
                     GSAWYRVGLEQDALSAYLEQKRKDTGQEPSVTEVEHYLEQTYYAWISRAFHRDFHSIY
                     CVVSYLWLLYYQIRNLFRIIDGRRYGLAADEIIRRMICES"
     gene            complement(12694..13002)
                     /locus_tag="PLA58_03320"
     CDS             complement(12694..13002)
                     /locus_tag="PLA58_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61858.1"
                     /translation="MDELITRIVAIERQCSADVEQVRQEYANNIESRKQSLEDQKTRA
                     FAQITAAEKLRLTKAMEEAKRQTEEASLAFQRDCEIPFQNQVLAQTIKEEIISILFEG
                     "
     gene            13296..13625
                     /locus_tag="PLA58_03325"
     CDS             13296..13625
                     /locus_tag="PLA58_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61859.1"
                     /translation="MEEYVHVPSGGEISFMAVTLKVSAEGCLACGNRQLLYVEGITTV
                     GSVCCGAVECRVIYVPGFIVSRHCKRDEASGHSVSRVEPVTDPATLEEVKKLLFKTFP
                     SSLFLLA"
     gene            complement(13757..14710)
                     /locus_tag="PLA58_03330"
     CDS             complement(13757..14710)
                     /locus_tag="PLA58_03330"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HOR61860.1"
                     /translation="MELTMKEDRLKNLTLQQMEALIFLVEERSFSRAAGRMFLTQPAM
                     TKHIRNVEDCLGVKVVNRGSGGVSLTPEGKILYDVARRIVRLRRDAADKIDQLREQSG
                     GDVYLCASTIPATYILPRAISAFKKEHPDIRIFLKTEDSEEVMNMVLDREVEIGCIGK
                     SPQNAKLAGQPVWHDRLILVVAADHRWAKKGSVSVSELMAEPFVLREKGSATRDVFEL
                     YMKEQKAVRLADFNICGELGSSEAIKEAVLAGLGVSVLSAHAVARELASGMLLEIPLA
                     SCRIERRFYLICLKSFEPRAHHKAFFDFLKHYKVAPAGLKR"
     gene            14920..15708
                     /locus_tag="PLA58_03335"
     CDS             14920..15708
                     /locus_tag="PLA58_03335"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOR61861.1"
                     /translation="MKLTDMFSLKGKVALVTGGGRGIGKFIATGLAEAGADIILTSRK
                     IKNLEATAAELMANQKVKAAAIACDMAKEDTVEAMLKEAVAKFGRIDILVNNAGATWG
                     APTLDFPLEKWDQVFNVNVRGVWVLTQKVARIMKEQGGGNIINISSVMAFRGSLEEAH
                     PAVPYNSTKAAINLLTMNLAVKLAPYKIRVNAIAPGFFHTDMMAYIDKPEFKPIRDAI
                     VNEIPLRRVGEIDDMKGVAVFLASDASQFITGHVMVVDGGQIAK"
     gene            15727..16623
                     /locus_tag="PLA58_03340"
     CDS             15727..16623
                     /locus_tag="PLA58_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61862.1"
                     /translation="MAASGQPAVPKESATVILVRSHPADAWNVFLARRHSRSSFMAEA
                     YVFPGGQIADADRDEKLQNCISMPAGFDPRALLQDDTLSPETAQSLFVCAIRETFEET
                     GILLARTRDGSPLNLTSEERNARFTAYRRELNAGRTTLGEMARLENLVFPLDALAPYA
                     HWITPEFLPKRFSTRFFLAALPEDQSAATDQGELTDSLWAKPEEFLRRYRGREIVLMP
                     PTLKTLEELARYACLNDLFAGTRGRAIYPILPEPGENMIKLPHDPEYGIERYKQPARP
                     EEPSRILMTDGLWRTGFDHEGT"
     gene            complement(17090..17494)
                     /locus_tag="PLA58_03345"
     CDS             complement(17090..17494)
                     /locus_tag="PLA58_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOR61863.1"
                     /translation="MGVIFDTSEIITLERNRGQIEEIVLEREDELFGISVITVAELLH
                     GVERADTETRKIKRQAFVEKVIEFFPVYSVDVTVARIYARLWASLAQKNLTVGAHDLI
                     IAATAMALNYTVITSNLRDYKKIEGLKIEEKN"
     gene            complement(17485..17763)
                     /locus_tag="PLA58_03350"
     CDS             complement(17485..17763)
                     /locus_tag="PLA58_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HOR61864.1"
                     /translation="MIKTISATDAVRTFSELLNAIKYKGEFYTIIRGGKPAATIGPVE
                     AVTSMRTLKELALVLKAMPKLDDDDKFAADVEDIITNQPVTPEGSSWA"
     gene            complement(17940..18026)
                     /locus_tag="PLA58_03355"
     tRNA            complement(17940..18026)
                     /locus_tag="PLA58_03355"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17990..17992),aa:Leu,seq:cag)
     gene            18138..19070
                     /locus_tag="PLA58_03360"
     CDS             18138..19070
                     /locus_tag="PLA58_03360"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOR61865.1"
                     /translation="MIVFEGIESITQDYRGSFVTIGNFDGVHLSHQHICRKLATEAKK
                     AGQKSLVITFDPHPKMILHPNIRPFYLITTRAEKLEFLEKCGVDATVVIAFDQHYSKT
                     TAEAFVQDFLWGKLAISKIIIGHDYVFGQARQGNDAFLKTKGRKLGFSVEVIPAFKID
                     DEIVSSTLIRNCILEGDLRKATKLMGRPYNVAGAVGSGAGRGAGLGFPTANIEPEKEL
                     LPPPGIYAAFVTADGRRCMGALNIGAKPTFEDYSSTFEVFLLDYSGDLRGKKINVLFV
                     EKLRDIVKFDGPESLKRQIAADVEKTRQILKQEK"
     gene            19088..20167
                     /gene="asd"
                     /locus_tag="PLA58_03365"
     CDS             19088..20167
                     /gene="asd"
                     /locus_tag="PLA58_03365"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOR61866.1"
                     /translation="MLKIAIVGATGIVGQQAIVSLLGHPWFEISKLVASERSATKKYI
                     DALRDANGSIRWWCPEELPEHIANMVVEEAASFDPTSVDIIFSTMDAGPAKELEPKYA
                     KTTPVISTASAFRYEADTPILVGGVNMDHAALLDVQRKNRGWKGFISPKSNCTIAGLV
                     VSLKPILDHFGVKQVIATSLQAMSGAGRTPGLSAMDMMENVVPYISGEEPKVETEPQK
                     ILGKLAGNKIVNAPFGITATCTRVPVIDGHLVVAFVQTEKPCTPEDVKKVMTEYGKDF
                     AELHLPSSPAHLIDVTDDPFRPQPRMDRDKGGGMTVTVGRIRKDPILENGIKYVCLAH
                     NTKLGAAKGALQTAEYLVKNYLKLV"
     gene            20676..22796
                     /gene="aspS"
                     /locus_tag="PLA58_03370"
     CDS             20676..22796
                     /gene="aspS"
                     /locus_tag="PLA58_03370"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOR61867.1"
                     /translation="MQFSERVFCGELSSEDIGRRVLLAGWVDAFRDHGGLLFIHLRDR
                     SGIVQIVFSPDTATGDICRQAALLRSEYCIAVSGDVRKRLAGTENPNIETGPIEVFVT
                     ELAVLAESDALPFSVSDKAMVAGAPSAGADHVAEDLRLQYRYLDIRRPAMRDNLMARH
                     RIFRCVREFLDSRGFVEIETPVLTASTPEGARDYLVPSRIHPQNFYALPQSPQLFKQL
                     LMIGGMERYFQLARCFRDEDLRPNRQPEFTQLDIEASFIDEEFLYELIEELTVRMFAI
                     GGIDLPRPFARMSYMEAMDTTGSDRPDLRLGLRFVDVTDVFSETRYSIFRQILQRGGC
                     IKGINIQGQSEKLSKNVLQNEYAREIAPSFGAKGMTWMRAEGGKLESNIVQFFSEQEL
                     AELKRRFAVTDGDVLILIADPSYAVVTSALGQLRLHLADRLGLIPENSFYPVWITEFP
                     LFEPTDEGGVTSSHHPFTAPDREDFDPKNLEELLSLKSRAYDLVMNGEELGGGSIRIN
                     NRDLQRKIFAALGLSEEETREKFGFFLRAFDFGAPPHGGLALGMDRTVSMILQTPSIR
                     EVIAFPKNRSAACPLTGAPSAVKREQLAELGLLNPGGQDVLPGTAEKEDPIDHLSWVS
                     RIGISAAERPVMEEILAGAEALARLAKEKAGSEEPIRTVAPVSNRMRPGTREMRSKLA
                     ESGKLLKSAPSVKGNYFKVASILE"
     gene            22812..24008
                     /locus_tag="PLA58_03375"
     CDS             22812..24008
                     /locus_tag="PLA58_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOR61868.1"
                     /translation="MDSIFFYRNPDSAASGDGALAGLNIAIQPNISKAGWPVDAGSRA
                     LANFTALEDATVVDRLSRSGAFLCGSTKTSEFGFGLAESRAGEAVKQKIADTELVLDM
                     TGEARLAAAAAGVCGFKPSYGLVSHFGLIGLIPSMETCGVLSSSIQNIRGILKAVAGP
                     DEKDFSLPDESVPDFTAQLINPTKTAIGVPAEAQSDLTDEQRERFRLSIEELSQAGFA
                     VREVSFPEWPLFLAVHKIVGSVEASSAAGRYDSVRYGERAPGAKNWNEMYLASRGASF
                     GTLLKSFLFQGAFFQFQRYTAYEDACRIRARLVADMKNLTGEVDFLALPVSGGASDPN
                     PATLDETYRQFACTAFANVTGQPALVLPPASKGQAPLQLAGPRLSDAGLLSLGEHLLK
                     LREGGK"
     gene            24011..25447
                     /gene="gatB"
                     /locus_tag="PLA58_03380"
     CDS             24011..25447
                     /gene="gatB"
                     /locus_tag="PLA58_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOR61869.1"
                     /translation="MEFEAVIGLEIHVELNCATKMFCDCPNNPGDDPNTNICPTCLWL
                     PGAIPRFSAEALEKASLLCLGLGAELQPKSAFDQKVYYYPDLPKGYQLSQAHLPLARG
                     GGIDISDEDGKTKRLRIHHIHMEEDVAKLVHEMEGRLPISLVDFNRAGSPLVEIVTEP
                     DFRTPHHAMEFLKALRTQVRYVGSSECSMENGTMRVDANISVRPKGTRQMNTKVEVKN
                     MNSIRHVGDAIAYEISRQSQAVQAGEAVVLHTRLWDPEKKVTLPMRGKFEGPCVPDPM
                     VPVIELTPEWIEKMRVRLPEMPAARAGRFVMQHGLTGEEAAFLSEDPEVAVYFEALVV
                     RGIAPRTAMHWLATQLLPAVKERKMELASQPVTPERFAALLKMLAGDEINANAAREVL
                     THLFESGESPEEIVKARGFKQVSDAGALEAMIDQVLATQAAAVADFRAGQGKALGFLI
                     GQVMQASGGKANPKIIRELLAKKLNAVP"
     gene            complement(25578..>27202)
                     /locus_tag="PLA58_03385"
     CDS             complement(25578..>27202)
                     /locus_tag="PLA58_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOR61870.1"
                     /translation="GKHEGRVATRFPPEPNGYIHIGHAKSVCLNFGIALQYSGTCNLR
                     LDDTDPAGESMEYVESIIHDVRWLGFDWGDRLFYASDYFEKLYAFAVELIKKGDAYVC
                     DLNSDEVRELRGTFTEPGRESPYRNRSVEENLSLFERMRAGEFPDGSHTLRAKIDMAS
                     PNIVMRDPVLYRIKREPHYRTGDRWVIYPMYDFAHCLSDALEGITHSICTLEFENNRP
                     LYDWIVEKLIAGNRPRQIEFARLNLSYTVLSKRRLIDLVRNRNVKGWDDPRMPTVTGM
                     RRRGYTPEAIRNFCAQIGVAKNDNLIDVSLLEHCVREDLNEKAPRAMCILRPLKVIID
                     NYPEQQTEDFDCANHPQHPEMGTRKVSFSRELYIERDDFLEDPPKKYNRLGPGREVRL
                     RNAYIIKFESMRKDEKTGEVTEIHCSYDPATRSAPPADGRKVQGVIHWVSAVHAVPAE
                     VRLYDRLFTIEDPGSEEDFLSCLNPNSLETLSACLAEASLKDARPGAQYQFERLGYFC
                     ADPDSAAGRPVFNRVVPLRDSWAKIAGGLKKG"
BASE COUNT         5794 a         7924 c         7503 g         5981 t
ORIGIN      
        1 cggttcggat cgaagtggtc cttgacgagg tcgcgcgcag gcgccggccc gaattccacg
       61 ccgtcgtaga aacctcccgt gctgcagaag aacattgcct cgccgggaaa ttcccgctcc
      121 agccggcggt agaggtactc gcctgtctcc ttcgattcgg tgaagaccag gagcttcttg
      181 cccttgagaa tggcgtggcc ccgcagatcg gtgatgaact gctcgatctt gggatcgccg
      241 gtgatcccca tccagattcg cttgatctcc ttcaggagat tcaggtcatc gttcagccgg
      301 gtgaggaaat cgtcggtgaa ttcgtctgcc gtatatttct gcacccggtc ctgttccacc
      361 agattgagca gggcctcctc gttgtcctcc tccaagaagt cgtacacatt tacccgcttg
      421 ctgatgtaga ccttgccctg ttcgatcatg gcaatgaact tctcatagga cgtgatgaaa
      481 cgcttgagcg tgttgccgaa ggcgtggaag ctgctctcca ggcgcttgac caggatgcct
      541 ttcatgaatc cgccgatgtt gcgctgagac tgtagctcca gagggtcgag ctgcttcttc
      601 aggtagagca agggcgtgta gcgggcatag cagaacttct tgagaagctc gatggtggca
      661 ttgaagatcc gctcctgccg ggcgtcgaag gtgtagatca ggcgctgggg atcgcccaac
      721 tcggggaagg agagcccctg tttgcttatg tcgtttttga aaaaattgac gacctctgaa
      781 cgggtgcggc ggaccatgac gtatttgagc accttttccc ggacgtcccg cgagacgtcc
      841 ttgatctctt ccgcataccc gggatccgtc ttggcatacc ggtccaggcg ggttttgaga
      901 ccggcgaaga acttctccag atcggcaacc ccggggattg tgctcttctt ggggatctgg
      961 aagagcttaa gctggctgta gatgtcctcc acggtgttgt tgagcggcgt ggccgaaacg
     1021 agaatcactt tcttgccgaa acaaatctgg tggagcttct cgtatccctg tgtcacttcg
     1081 ttgcggaatc ggtgtgcctc gtcgatgaag atgaaatcga acttggctgc cccttctttg
     1141 atgacatgat cgagctttcc cagggactcc accttgtagc cgcggacgcc gaattcgaag
     1201 aaggtctcct cccagtaatc cttcagcacc ggcgggcaga tgaccaggat cttgccccga
     1261 agctgctgag cgagcatggc ggagatgaag gtcttgccga ggccgaccac gtcggcgaga
     1321 aagacgccgt tgtaagcgtc gaggatcttc ttcgcggcta tcaccgcctg cttctgataa
     1381 tccaggtcca tgaaaccttc cgggagatac gtttcaaaat cctcgtcgat gttgatgtcc
     1441 tcccggaagt gctcataaag gaacttcagg tagaggtggt agggagtgat ctcgtcgttg
     1501 agccaagtct tgtcgtggat ggtatccacg tactcatggg agagatcgac cgcgtccttc
     1561 cagagctcct cgaatcgggc cagggcgaat tccacgtccg cccggttttt caactccacg
     1621 ttgaactccc ggttggcgat gagccccgcc tcggagaagt tgctggaacc ggtgatcacc
     1681 cggccgaaat cgcggtcgtc ctcgccgaat cggctgatat aaaccttggc gtgaaggttg
     1741 ccgctggggt aggccttgat ctccatctta ccggagcgca ggaactcgat gaacttgcgg
     1801 acacccagtt cggtctcgta gctgtccggg gaacagtcca tctcttcggt aactgccgag
     1861 gagaagattt cttttgttcg tttgtgggat tcgaaatcca gtgcagactg agtctggaag
     1921 gcgtcgatga tctcgaaggc ctgacgatcg acattcaggc cgacgaggat gcggatcttg
     1981 tcgatagagg cgaaggactc gtggagtttg tcaaaaccac tggtgcggaa gtagccgacc
     2041 agaatatcaa aatacttcac atcccggagg gttctcctga accggtcaag caacgtggaa
     2101 tctggatcat tggtgaaaaa tgtcaggtcg ttttgcatga aatgattctc gctccaaggc
     2161 atgaaatatg ccatgcaaac ctgcagcttc atagcagata tcagattgga atgccagaac
     2221 tttgcaataa atgaagttgg ctggcaagat gcgcgtcggc catttggaat gaccgccacg
     2281 cccctgttca caagctttct gaccaggcgg gtggtcggtt cttcaaggct gaggatcctc
     2341 tggacatctt ccagcctgat ttttgaacta gcccatcatc gaggatgagg aagcggcaac
     2401 ggtggttggc gaagatacca tccctattgg gcttcccatg gattgaagac ctttgtccgg
     2461 cagcgctcca tgtctttcac gttgcgcgtg acaacgataa ggtcatggac attggcagat
     2521 gcggcaatca ggctgtcaac gacaggcagt ttttctccca gcctctccgc ttccccaagc
     2581 atcgttcccc aagccatgga gatttctgcg tcgatctcca agagacgtga accgaatcgt
     2641 tgtacaagat cgtttgtgac ccaggattgc agtttttctc ttctgtcacc gttcgggagc
     2701 ttgttgatcc ctttaatgat ctcaccgagg gtcagtacgc tcaaatacat cttgctttca
     2761 tcgccggtat ccatccaccg aatcaccgaa ggatggggct ccttctttac caactccgag
     2821 atcacgcagg tatccaggag gtatttcata ggtgaacctc gcgggacaca tccttgcttc
     2881 tctcgagatc gagatctgtg ccgtaaagtg gcgattgcct gagaaattca cgcagcgacg
     2941 tgccggggcg tgtgatattt tggaattcct caaaagatac aacaaccacg gcaggcttgc
     3001 cgcgcaatgt gatggtttgt ggaccctcgc gaagcgcgtg ttccactact tgactcaaac
     3061 ggcttttcgc ctcctgcaat tgccaggagg ggtccttttt ttttgtattt gttctcgtca
     3121 tagcggcttc tcttcctgtc tagtctgacc agtcattttg atttctcata tcatagggcg
     3181 tcttatggcc gcaacaacat ttttcaacgc gcccacaggc ataaacgaat ctttccccaa
     3241 gtcgcagtcc aatattggag agcttgcaga accctataaa tccattgctt ttgtcataca
     3301 ggagtaggtt tgtgtccagt attatcaaat atttcctgga ttcccgcctt cgcgggaatg
     3361 acctacaatg gagtcgtgca aacgtctcta ttgttatcca atgcggaaat gaacacccat
     3421 gaaatctctg cgatattcgt tttgctattt caaaatttgt caattctcct gcggcatccg
     3481 ctcctggtaa taatgcgtga tatcgaatgc cgcgtagtcc atcagcagca tgatgcgcat
     3541 caggctgtcc acggcctgct gatgcgccag cgcgttgacg aacgtccgct gaaattcgtc
     3601 atgaagccac atctggcggc gcatgacatt gagggcataa atggcctcgt gcaggggaat
     3661 gccctgctga cggcaggttt ccgcgtactg ccgaaaaaat tcacggcctt tttcaaagcg
     3721 ttcgggatcg aaaagaaggg ttctcaggtg gctgtaaaat ttaatcgcct gcggcattaa
     3781 tttttccttg gggatgttat gataataaac cgtctgcctg tccttccgga cggtcgcggc
     3841 ccagtttgca gcgatttcca ccacattgga ctccaggata tccagcagct taatcgattc
     3901 cagtttgtcg cgcttcatca attcctgata ttcccgcgtc acatcatagg aggcgtaatc
     3961 aaagagcaga atggtgcggt tcagggtatc caacgcctgc tgctggttga tgagggagct
     4021 gaagatcatc tgaaactcgg cataaagcca gatgtgccgg cgcatcagaa tcagggcgta
     4081 gaccatctcg gccatgggaa tgcccagggc gtagcagtcc acggcgtagc tcctgaaata
     4141 tttctggacc tcttccgaaa ttttttcatg aacaaacatt ttgctgaagt tccgatagaa
     4201 ctgaacgcac tggaaaatga ttttctgttc gttcagattt ttataggtcg gcgttttggc
     4261 gttgttttgc acatccaggg cccatcgttt ggccattttc tccgcatgct gttccgccag
     4321 ttccacatat ttcagggtgt aggttctcat catccatctc ctttctttta tggattcggc
     4381 tttgattcct tcgtcattcc actctggccg cgctgccgga tgagcttttt tacaaagacc
     4441 tcttcacgct ccatttcctc aatcctgtcg gtgatgtact tcttcgactc ctcatgctgg
     4501 ggaataaaga tcttctccag agcgttgacg cgccgctgca ccttcttcaa ttccctggag
     4561 agcatcatga cggacttggt catggtcgca tactcggcaa gggtgagcag cgtttttcgg
     4621 gattgatctc tcgcttcgtc ataggcggcg gtggtttgag gcagcgtgaa ggcaggatgg
     4681 ggatttttga gccgaagccg gatcaccggc atccggaggc ccatgagttt tgtaaaatcc
     4741 gtccggagga aatagctcct cacttccgag caactctgcc gggacgccac atcggacccc
     4801 atatccacag ccgcgctgcg ataagccccg tagagctgat tcaaaccctg ctggaattcg
     4861 gcctcgatcc tgcgaatttc agcgatcttc cgcacaatct caatggccag aatttcccgc
     4921 ttctgattga gaaggtcgta cccttcatag gcaaaggcca ggtcttcctt gagcctgcgc
     4981 agggatgatt tcgtcggggg caattctatt ttcggcataa tttcatcacc ggcttgccgt
     5041 cgtttaattc cctccctcca cggaagggat tggaggaggg tgaaaataac attccccctc
     5101 cccgcaatcc ccgcccgccg ggggcgggga agttctcatc agggaacggc tgtcggcctc
     5161 gcgccacctt cagggggtca ggtggttagg tgctaggcaa tcgcgaccgt tccctgcatc
     5221 ccccgattcc ggatcgagcg tcccgggccc ggaataacat caaaacgaaa ttctctttac
     5281 gcgcttgcgg cctcgggcat gtagtatttc tggatgtcct ccctcgcaat gcggtacaac
     5341 tcgtcgcgcg gcaggagcga aagcatccgc cagccgatat ccagcgtctg ttcaatcgag
     5401 cggttctcct cgtaatcctg ccgcagaaaa atctcctcaa aggccgtccc gaatttcaga
     5461 taaactttat ccagatcgga aagctcctcc tctccgacaa tcgccgccag cgcccggatg
     5521 cgcttgactt tcgcatacga ggcgaaaagc tgggcggcca cctggccgtg gtccttccgc
     5581 gtccgccctt ccccgatgcc gtctttcatc agcctggaga gcgacggcag gccggcaatg
     5641 ggcggataga ttcccttgtt gaaaagcgcc cgctccagaa cgatctgccc ttcggtgatg
     5701 tagccggtca gatcgggaac gggatgggaa atgtcgtcgc tgggcatcgt caggatcggc
     5761 acctgcgtaa tggatccctt gccccctttc agccgtccgg cgcgctcata gacggaggcc
     5821 agatcgctgt acagatagcc gggatagcct ttgcgcgaag gaatctcgcc gcgagaactg
     5881 gacagctccc ggagcgcttc gcagtaattg gtcatgtcgg tcaggatgac cagaacgtgc
     5941 attccctcct cgaaggccag atactcggcc agcgtcaaag ccgtgcgcgg ggtcaccagc
     6001 cgctcgatgg agggtgaatc ggccaggctc aaaaagatgg cggagtgcgt gagcacgccc
     6061 gattcttcca gcgaatcgat gaagaaccgg gccacgtcga acttgacgcc catggcggca
     6121 aagatgacgg tgaattcctc ctcggcggtg agaatcctgg cctgccttgc aatctgcgcg
     6181 gcgatccggt tgtgcggcat gccgctgccg gagaaaatcg ggagcttctg cccccggatc
     6241 agcgtgttca ttccgtcaat cgccgagatg cccgtttgaa tgaagtcccg cggatattcc
     6301 cggctccagg gattgatcgg caggccgttg acgttcatca tccgatccgt aatcacctgg
     6361 ggggcgccgt cgatgggccg ccccaggccg tcatacaccc ggcccagcat gtcgcgatcc
     6421 acgggaattt cgagggcacg gcccacaaag gtgacccggg tgccttttac cgaaagcccg
     6481 ctggtgcctt catacacctg gacgacggct ctctgttcat ccacctccag cgtctgaccg
     6541 gtgcgcagct cgccagacgc gtcccggatt tccacgatat cgccgtagcc gacgcctcga
     6601 atcccatcga caaaaatcag cggtcctgcg attccggaca gattggtgta ttcgatttcc
     6661 gcggacatat tacgctcctt tcgacggcgc tgaaagcgag gcaagcttct gttccagccg
     6721 gttcatatac tcgtcgattt ttccgatgtc ctggttggaa atttccgatt tcagccgcac
     6781 cagacgggac gcctcttcca gcgcggcaat ctccttcacg ctcacgccgg cgcgcacttg
     6841 ctccaggcct ttcttataaa ggagaagcag gctcgccagg attctgagct gcttttcggg
     6901 gctgcagaat ttatcctggg ggtcgaagga gttctgctgc aggaaggcgt tcttgaccga
     6961 ttcggcgcag aaaagaatat attgctctgc gggcggcagc gcgtcgcttc cgaccagctt
     7021 caccacctgc tcgagccggt gatcctcgag caggatattc atcacttcat tccgggtggt
     7081 cagccactgc ggatcgagat ttttccacca gtcctcgatg tcggagaggt attcggtata
     7141 gctgtcgatc caactgatgg aaggatagtg gcgcgcgtcg gccagcgtct tgtccagcgc
     7201 ccagaagcac cgcacaaacc gcgaggtatg ctgggtgacg ggctcggaga aatctccgcc
     7261 ggggggcgac acggcgccga tgatggtaat gtcgccgtct tttcccgaaa gcgtttccac
     7321 cagtcccgcc cgctcgtaaa actccgccag cctcgtggcc aggtacggcg gaaatccctc
     7381 ctcggcgggc atgtcgccca gacggctggc cagttcgcga agggcctccg cccagcgcga
     7441 ggtggaatcc gccatcacgg ccacgctgta acccatatcg cggtaatatt ccgcgatggt
     7501 cacgccggtg tagatgctga cttcgcgcgc cggcaccggc atattggatg tgttggcgat
     7561 catcaccgtc cgttcgatca gcggccggcc gtttctctca tcgatgagct tcggaaaatc
     7621 ggtcaggacg tccgtcatct cattgccgcg ctcgccgcag ccgatgtaaa cgatgatgtc
     7681 cgcgttgcac cacttggcca gcgcatgctg ggtcatggtc ttgccggttc cgaagccgcc
     7741 ggggatggcc gccgtgccgc cgcgggcgat ggggaaaaac gtgtcgatga cccgctggcc
     7801 ggtaacgagc ggaatgaacg gtttctttct cgttttgctg gggcgcggtc tgcgcaccgg
     7861 ccagtaggcg gcgagctttc cttcgtaggc gcccgcgtcc gtatcgatca aatagatggg
     7921 ttcttcaatc gtgtaagacc cggccggggc gatgcgggtc agcgtgccgg aaacgccggg
     7981 aggaaccagc agccggtgca gcaccagcgg gctttcctga acttcgccca ccagggctcc
     8041 ctcaccgacc tttgcgccgg atgacacaag cggtttgaaa ttccatttcg ctttcgcatt
     8101 caggggagag agcttgatcc ccttccggat ataactgccg cacgcctcgg agatcccgac
     8161 caggggccgc tgaattccgt cgtagatgct ggcgatcagg cccggcccca gaaggacgga
     8221 aagcggccgg ccgtaggaaa tcacctcatc ccccggcttc aggcccgtat tgtcttcata
     8281 cacctgaatg atggcgattt cctccgccag gcggatcacc tcgccgatga ggcggtccgg
     8341 tccaacctcg gcgatgtcgt acatccggat ccccgaaagc cccacggact tgacaatcgg
     8401 gccgttgacc gaaatcactt ttcccgtcag cagtttttcc atgttcactc ctgaaaagcc
     8461 gccccgcgcc ggttcagggc gccggaccgc aggcaacgat ggaaagcggc cggaccgtta
     8521 tcgggtcacg gattccttcc cctccgttct gtcctgcaga acctgcagga cctttcgccg
     8581 aatctgcaaa gccttccgga aataaattct ttccagcgtg cgattgaaaa tgcgtccccg
     8641 ggcagaatcc accaccaggc agccgcccca cccgatcgcg gcatcggcct caatgcgaat
     8701 ctgccgatgg tcccctgcga tacggagagc ctcgtcaatc tcgtttccgt aaactccgtc
     8761 gtccggtgca agccgcactt ccacattttc gggaatttgc gcggcggcgc cggctgccgc
     8821 atccatgaga aattgccggt agcgcggatg gctccggatc cctctcatca catcctcgac
     8881 aatccgttga atgaatcgct ccaccgtggt cagcttgagc ttttttctct ccagaacggc
     8941 tctgttttcc agccgggaaa gctcctgatc catgcgcgaa cgcgtttcgg cttccatcgt
     9001 tttgcggaaa cgctcaatct ccgccgcata atcctcgtcc agccgcccaa gctcgcgggc
     9061 ttccttctcg cggatttcgg caatcatttc ctccgacgcc cgtttgatgg aattttccag
     9121 ggaggtgttg tccatattca atccttcttt gtgtcgggaa cggtttcaaa ccgttcccga
     9181 cagtgatccg gcgatgatgc tcacaacgat atccccaggg cctcctccac gtacgccatc
     9241 accgatccgc ggaagccttc cgggtcgtcc atgccgggaa tctcgaccac caccggggac
     9301 ggcctgccca ggttgatttc cgcaatgcgc tccccgagtc cggaagccag cgcgccggtg
     9361 atgagcacca ctcccgcgtc ttttttcccg acgatccttt cgagcgcggc ggcggcccgt
     9421 gaaggctccg ccacgacgcc ctccacgccg gccagacgaa aagcgctgac ggtatccatg
     9481 tctccgatca tgtaaatttt ttccatatta cagcttattc aggatcatga tggcgataat
     9541 cagtccgtaa atggcgatgc cttccgccag cccgagaaat accagggtgc ggcccatcag
     9601 ttccggtttt tcgctcaccg ccccgatggc cgcggagccg atcttggcca ccgcatagcc
     9661 gctggccaga caggcaaacc ccacggccac ccccgcgccg atgtaagcca gcccgatcgc
     9721 ttccgcgagc gctttgaccg ggcttgcctc ggacgcccat cccatggccg gaaatagact
     9781 ccacagggcg tacaggaaaa tcgcccccat cagaaacgcc aactgcctgc tcctggtcat
     9841 ttcacacctc cgatgctttt gccggtaacg taaacgggga aaagaccacc ccgtctccct
     9901 tgaaaaactt gctgaaaaac tcgtaatatt cgagacgcaa ggactgaatg ccgcaaatca
     9961 gtccctcgaa gccgatgatg aaaatattgc cgcacacgat gatgatgacg gcgcctacgg
    10021 atttcaggga cgggtcggcc atccccgcaa gcgtataggt gacaatgctc aagcccgcgt
    10081 gcgaaagcgc aaaggcgccc acgcggataa aggaaatggt attggcaaaa agacccagcg
    10141 caatctccac cacctccatg atggtttcaa cgatgtattc cgatgggctg tgcggtttgg
    10201 ctttttccaa aaacaacaca cccagcaggc cgcgcagcga aaagatcatc aggggaagga
    10261 ccataaaaat ccccagcgcc cataaggcgg gaggctgacc ggtcttcagg tactgcagga
    10321 cgaatacgac ggcagccgcg taaacgacga ggatggccag cccccgcttc tccaggaggg
    10381 cttcggaata atccgcattg acaaaactgt tgatgatgtt gaggcaaagg ccgaccagga
    10441 taaacaccac gcccatcatg atggtgaaga aaaacaggcg catgatctga gccgtggggt
    10501 gaaaccacag agcgggcagg aggtgctcgc tggaaaaaac actgccgtac agcagaccgc
    10561 agacggccgc cgagccgccg caggccagaa gaatacctcc cgcctgggcc agttcttcgc
    10621 gggctttctt ctgaccgaat tttttcagca gccagccgca cagcatcagc acgatgccct
    10681 ggccgagatc gccgaacatc agtccgaaca tcatgacata ggtaacggcc gcaagcggcg
    10741 tgggatccag ctcgtttgaa gagggcatcc ccatggtttt gacgagcagc tcaaagggcc
    10801 ggaacagcca caggtttctc aatcggacag gggcgttggc gtccttctgc tccgacacga
    10861 caaacccgcc gccgcaaacc tccttcatga tttccatgag gattgccttt tcctttgcgt
    10921 ccacccaccc cgtgagaaac atggcccgcg atgaaatcac ggacaggcgt atggctttga
    10981 ggacttcctc ataggcccgg taatcggcgt tcatccgccg gagaaactct cctttttcct
    11041 ctttcaggcg gttgacgtag gcatccagcg ccgcaagacg ccgccgcaaa atatccgcgc
    11101 gcttttccag cgcagcggaa gaaatagtcc ggacatcctt cgcttggtcg gcaaatccgt
    11161 tttccttcag aaactgcatc atgtcggcgg caaaagccgg cagggccatc ccgaacacaa
    11221 agcgtcccgt cctgacgatc ataaaacgct cgtcggacgg aaccagccag tcggtctgtt
    11281 ccaccgtccc gaagaccgtt tgaagaatgc ccggtttttt cagcatttcc gcgtcgatgc
    11341 ccatgcgctc cagcgcccgg gcgtattcaa tgtcccgtgc cagaatttcc cgctgatccc
    11401 ggatttttgt ctgaagccgc agcgcgcgct ccagctttct tttcacgccg gaagcctccg
    11461 cttcatcctg cgccgtattc accagcctgg cctcccctcc gcgctcatcc ggctccagat
    11521 gcagggcggc aagagtataa gcggttgccg aaaggatctc gcgggtcagc gactcctcgc
    11581 cctgcagtcc tccgtccgcc aaggaatctt tctcgtgaaa acgggcaaga tgaacgagtc
    11641 ccgccttccc cagcgcaaga taaaccctgc gctgatgttc tttgtcgaaa gcgatcgtta
    11701 ttttccggat atccgatttt acaaacatac ccgacctcac gattcacaga tcattctgcg
    11761 gatgatctcg tcggcggcaa gcccgtagcg ccggccgtcg atgattcgaa aaagatttcg
    11821 aatctggtaa tacagaagcc acagatagga gaccacgcag tagatggagt gaaaatcccg
    11881 gtggaacgcg cgggaaatcc atgcgtagta ggtctgttca agataatgtt cgacttccgt
    11941 gacggagggt tcctgccccg tatctttcct tttctgctcg agataggccg aaagcgcgtc
    12001 ctgttccaga ccgacccggt accaggcgct gcctccgtaa agcgcctgaa acttatccat
    12061 gtagaaccgg actttttcct gcgggaggcg gtgatgcacg ccgagccggt acgaccagat
    12121 cagcgtcatc acggccagcc ttttgagcat catttcgcga aattccgcgg cggcggaacc
    12181 gacaagcaat tcggcggatc ggtaaagatt ccgggcggtg cagtcatcca ggtgaaccac
    12241 cagctgccgg tagccggaaa tggccctgaa cgtctcgtcc tgataaatgc cggccagaag
    12301 ggttttgacg tcgtcaagcg acagccgctc ttccagcagc cccctgtcca gtgcagcaaa
    12361 gggaccgata ttgtaccact ggcggaaact gtgatggccc gccgccgtcg ccagaagaat
    12421 cttggcgttg tgcgcctcat actggcggag atgggcgata aaaaacggcg tatatttttc
    12481 atgggccgcg accagaggga agatcggcgc aatctgttcc tcgaagagct tctccttggc
    12541 ctgtatcgga tcctgaattc cggagacatg tccgggcagc gtctccggct cacggatcat
    12601 caggatataa tcccgaaggg agaagaggcg gcttttcatg gcgtatatcc gcgtgtggat
    12661 attgggcaca tcggcgtaat ttcgcatcct tttctaaccc tcgaacaaga tggaaataat
    12721 ctcttctttg atggtttgcg ccagaacctg gttttgaaac ggaatttcgc agtctctttg
    12781 aaaagcgaga gaagcctcct cggtctgcct tttggcttct tccattgctt ttgtcagccg
    12841 gagcttctcc gcggcggtaa tttgggcaaa cgccctggtt ttctgatctt caagactttg
    12901 tttgcgggac tcgatgttgt ttgcatactc ctgccggacc tgctcgacat ccgcagaaca
    12961 ctgtctttca atggcaacaa tccgggtgat caattcatcc atgcctgcac cccactgatt
    13021 tgcctgctga atagttgacg tttgtttttt cttctgcctg aatcgatccg gatgaaaacc
    13081 gtaccgctgg actttgcgcc cgagagattt gcgtgtttgc tgtgttggtt gaaattataa
    13141 cggttcaaat tttacatgtc aacgaaaata tagacaaatg taaggataag gatgctccga
    13201 ttttaccttg ccgcaccgca tccccgtgca aagatttgat ttgcgccaaa gcggctggtc
    13261 agggtaaaaa gaaaaacttt atttcgggag tttaaatgga agaatacgtt cacgttccca
    13321 gcggcgggga aatcagcttc atggccgtca ccctgaaggt gagcgccgag ggatgtctgg
    13381 cctgcggcaa tcggcagctt ctgtatgtcg aggggataac caccgtcgga agcgtctgct
    13441 gcggtgcggt ggagtgccgt gtcatttatg ttcccgggtt catcgtctcc cggcattgca
    13501 aaagggatga agcgtcgggg cattctgtaa gccgggtgga gccggttacg gatccggcca
    13561 cgcttgaaga ggtgaagaag ctgctcttca aaaccttccc ctcgtctctt ttccttttgg
    13621 cctgaaaaaa actgtgcgcc accctggcgg ctcgagggaa aacgccgcgc aacccaggag
    13681 gctgcactgc aagcgcaacg atacggctat tgcgcgggca gccttcttag aggtggttct
    13741 cacgccgtgt atttcattac cttttcaatc ccgcgggagc caccttgtag tgcttcagga
    13801 aatcaaaaaa tgctttatga tgcgcgcgag gttcaaacga cttcaggcaa atcagataaa
    13861 accggcgttc gatgcggcag gacgccagcg gaatttcgag cagcatcccg gaagcgagtt
    13921 ctcttgccac ggcgtgcgcg gacaaaacgg aaacgcccag acccgccaga accgcctcct
    13981 tgatggcctc ggagctgccc agttcgccgc agatgttgaa gtccgcaagg cgaacggcct
    14041 tttgttcttt catatacaat tcaaaaacat cgcgtgtggc cgaccctttt tcccgcagaa
    14101 caaaaggctc cgccatcagt tcggaaacgg agacagaccc cttttttgcc cagcggtgat
    14161 ccgcggcaac caccagaatc agccggtcat gccagacggg ctggccggcc agttttgcgt
    14221 tttgagggct tttcccgatg cagccgattt ccacctcccg atccagcacc atgttcatca
    14281 cttcctcgct gtcctccgtt ttgagaaaaa tccggatatc gggatgctcc tttttgaacg
    14341 cgctgatggc gcgcggcaga atgtaggtgg ccggaatcgt gctcgcgcac agataaacat
    14401 cgccgcctga ttgttctcgg agttgatcaa ttttgtccgc tgcgtcgcgg cgcagacgga
    14461 cgatccttcg cgcgacgtcg taaagaattt tgccctccgg cgtcagggaa acgccgccgc
    14521 tgccgcggtt gaccaccttt acgcccagac agtcttccac gttgcggata tgcttggtca
    14581 tagccggctg cgtcagaaac atgcgcccgg ccgcgcggct gaaacttcgc tcttccacca
    14641 gaaaaatcag cgcttccatc tgctgcaggg tcaaattttt cagccggtct tccttcatgg
    14701 ttaactccat aacataaata ttctttatcc acaaagccta cgtcgccgga actctttttg
    14761 tatccttttt tcacattttg gttacgcctc ttgacggttt atgatagggt tctttctcaa
    14821 cgagctgttg cccaaagcta tctcatcagg caacagcagg tcaaattcaa acgtctccgg
    14881 agaaaatccg gcggacccta ttttacgaag gaggaaacca tgaaactgac agacatgttt
    14941 tcactgaaag gaaaagtggc gctggtaaca ggcggcggca gaggcatcgg caagtttatt
    15001 gccacaggac ttgcggaagc gggcgccgat attattctga cctcgcgcaa aataaaaaat
    15061 ctcgaggcga ccgccgccga actcatggcc aaccagaaag tgaaggccgc agccattgcc
    15121 tgcgatatgg ccaaggaaga cactgtggaa gccatgctga aggaagccgt ggccaaattc
    15181 ggccgcatcg atattctggt caacaacgcc ggggccacct ggggcgctcc cacgctggat
    15241 ttcccgctgg aaaaatggga ccaggtgttc aacgtgaacg tgcggggcgt ctgggtgctg
    15301 acgcagaaag tggcccgcat catgaaggag caggggggcg gcaacattat caatatctca
    15361 tcggttatgg ctttccgcgg ctcgctcgaa gaagcccatc cggccgttcc ctacaactcc
    15421 accaaggcgg ccatcaacct gctgaccatg aatctcgcgg tcaagctggc gccctataaa
    15481 atccgggtca acgccattgc gccgggcttt ttccacacgg atatgatggc ctatatcgac
    15541 aagccggaat tcaaacccat ccgcgacgcc atcgttaacg aaattcctct gcgccgcgtc
    15601 ggcgaaatcg acgacatgaa aggcgtggcg gtgttcctgg cctccgacgc gtcgcagttc
    15661 atcaccggcc acgtcatggt cgtggacggc ggacagatcg cgaaataaag agaaaaagga
    15721 gaaagcgtgg ccgcatccgg acaacctgcc gtgcccaaag agtcggcgac ggtcattctg
    15781 gtccgaagcc atcccgcgga cgcctggaat gtttttctgg cccgccgcca cagccgttcg
    15841 tcttttatgg cggaggccta tgttttcccg ggcggacaga tcgcggacgc cgaccgggac
    15901 gaaaaactcc aaaactgtat ttccatgccg gcgggctttg atccgcgggc gctgctgcag
    15961 gatgataccc tttccccgga aacggcgcag agcctttttg tctgcgccat ccgggaaacc
    16021 tttgaagaaa cgggcatcct gctggcgcga acacgggacg gaagccccct gaacctgact
    16081 tcggaagagc gcaatgctcg ttttaccgcc tatcgcaggg aactgaacgc cggccggacc
    16141 accctgggcg agatggcccg gctggaaaat ctggtctttc ccctggatgc gctggcgcct
    16201 tatgcccact ggatcacgcc ggaattcctg ccgaaacgct tcagcacccg tttctttctg
    16261 gccgcgctcc cggaagacca gtcggccgcc accgatcagg gcgaactgac ggacagcctc
    16321 tgggcaaagc ccgaagagtt tctgcgcaga taccgcggcc gggaaatcgt gctgatgccg
    16381 ccgaccctga aaacgctgga agagctggcc cgatacgcct gcctcaacga cctttttgcc
    16441 ggaacccgcg gccgggccat ctatcccatc ctgcccgaac cgggggaaaa catgatcaaa
    16501 ctgcctcacg atccggaata cggcatcgaa agatacaaac aaccggcccg gccggaagag
    16561 ccgtcccgca tcctcatgac ggacggcctc tggcggacgg gttttgatca cgagggaacc
    16621 tgaccgcaag tcagaaaatc ttgacaatac ggatttccct gcctatactt cacccggact
    16681 ttggggaagt cgcgtcggca acccgattcc ccccgtcaca caatccgatg aagcgttttg
    16741 cagcctttcc tcttccttgt ccgctcaata aactaccccg ccgcaagcag cggggtattg
    16801 tagaaggaaa ccctttaata atttcgcccc aaggagcggg gaatttaccc gtagagatac
    16861 ctatcacttt ccagtgtccg cccttatccg ggccgatacg ttttattaat tcggactttt
    16921 tcaatgtgat taaattcttt tttacggctc tgtctgtaat gctcagcaat accgaaagtt
    16981 cggcatggtc aaccccggat tatccttaag gattttcaat attttccggg aacttttccg
    17041 ggaactttca cccaacgttt ttcccggcgt ttccccatct ccgccaccgc taattttttt
    17101 cttctatctt caatccctcg atttttttat agtcccttaa attggaagtg ataacggtgt
    17161 agttcaatgc catcgccgtt gcggcaatga tgagatcatg agcgcctacc gtcagatttt
    17221 tttgcgccag agacgcccat agccgcgcat aaatgcgcgc aaccgtcaca tcgacgctat
    17281 agacagggaa aaactcaatg actttttcta caaaagcctg ccgcttgatt tttctggttt
    17341 cagtgtctgc gcgttctaca ccgtgaagaa gctcagctac tgtgatgaca ctgatgccga
    17401 ataattcgtc ttcccgttcc aaaacgattt cttctatctg cccgcggttg cgctcaagtg
    17461 tgataatctc actggtgtca aaaattacgc ccatgaagat ccctccggag taaccggctg
    17521 gttggttata atatcctcaa catcggccgc aaatttatcg tcatcatcca gtttgggcat
    17581 tgctttcagg acaagcgcca attctttcaa ggttctcatg ctggtaactg cttccaccgg
    17641 cccgattgtc gccgccggct tcccgcctct gatgatcgtg taaaattctc ctttgtattt
    17701 gattgcattg agcaattctg aaaatgttct cacagcatcc gtggcactga ttgtttttat
    17761 cataaccgtc tctcctgtaa aatatgattg tatgatttta cataattata aatattttct
    17821 caatgcattt ttgtcgatgt aagtttgttt ttgaaaggct tttcgaaaaa cgccggctgc
    17881 accggagaaa atcaaaaagg ggctaaacag atgttctgtc tcgcccctct attttcttat
    17941 ggtgccgaag actggatttg aaccagcaca ggcgtacacc cactagaccc tgaacctagc
    18001 gcgtctacca attccgccac ttcggcacgg gaaagtgact atactgaacg cggcatcttg
    18061 tcaagttaaa atgacttgaa tttaatgctc aaaatgctat gttgcaaaac ttgtaaacac
    18121 agacggcggg aaagaacatg atcgtttttg aaggcattga atccataact caggattacc
    18181 ggggatcgtt cgtcaccatc ggcaattttg acggtgtgca tctgagccac cagcatatct
    18241 gccgcaagct cgcgacggaa gcgaagaaag cggggcagaa gtcgctggtg attacctttg
    18301 atccgcaccc caagatgatt ctgcatccga atatccggcc tttttacctg attaccacca
    18361 gggcggaaaa gcttgaattt cttgagaaat gcggcgttga cgcgacggtg gtcatcgctt
    18421 ttgaccagca ttactccaaa accaccgccg aggcgtttgt gcaggacttt ttgtggggga
    18481 aactggccat ttccaaaatc attatcggcc acgattatgt cttcggccag gccagacagg
    18541 gcaacgacgc ctttctgaag accaaggggc gaaaactcgg tttttccgtg gaggtgatcc
    18601 cggcgtttaa aatcgacgac gaaatcgtga gcagcacgct gatccgcaac tgcattctgg
    18661 aaggcgacct ccggaaggcg acgaagctga tgggacgccc ctacaacgtg gcgggcgccg
    18721 taggcagcgg cgcggggcgc ggggcgggcc tgggattccc caccgccaac atcgaaccgg
    18781 aaaaggaact gctgccgccg cccggtattt acgcggcctt tgtgacggcg gacggccggc
    18841 gctgcatggg ggcgctcaac atcggcgcca aaccgacttt tgaagattat tcatccacgt
    18901 tcgaggtttt cctgctggat tattcgggag acctgcgcgg gaaaaaaata aatgtgctgt
    18961 ttgtggagaa gctgcgggac attgtcaagt ttgacggtcc cgaaagcttg aagcggcaga
    19021 tcgcggcgga tgtggagaaa acgagacaaa tactgaaaca ggaaaaataa tttggaggaa
    19081 aacgtttatg ttaaaaattg ccatcgtggg cgcaaccggc atcgtaggac agcaggcgat
    19141 cgtcagcctt ctggggcatc cctggtttga aatttccaaa ctggtcgcct cggagcgctc
    19201 ggccacaaag aaatatatcg acgcattgcg cgacgccaac ggctccatcc gctggtggtg
    19261 tccggaagaa ctgccggaac acatcgccaa catggtcgtg gaggaagcgg ccagcttcga
    19321 tccgacttcc gtggacatta ttttctcgac catggacgca ggccccgcca aggagctgga
    19381 gcccaaatac gccaaaacca caccggtcat cagcaccgcc tcggcgttcc gctatgaagc
    19441 cgacaccccg attctggtgg gcggcgtcaa catggaccac gcggcgcttc tggacgttca
    19501 gaggaaaaat cgcggctgga agggattcat ttcgcccaaa tccaactgca ccatagcggg
    19561 gctggtcgtg tcgctcaaac cgattctgga tcatttcggc gtgaagcagg tgatcgccac
    19621 ctcgctgcag gccatgtccg gcgcgggccg cacaccgggc ctttccgcca tggacatgat
    19681 ggaaaacgtc gttccgtaca tttccggaga agagccgaag gtggaaaccg agccgcagaa
    19741 aattctcggc aaactggccg ggaataaaat cgtcaatgcg ccgttcggca tcaccgccac
    19801 ctgcacccgc gtgccggtca tcgacgggca tctggtggtc gcgtttgtgc agaccgaaaa
    19861 gccctgcacc ccggaagacg tcaagaaggt catgaccgaa tacggaaagg actttgctga
    19921 gctgcatctg cccagctcgc ccgcgcatct gatcgacgtc acggacgatc cgttccgtcc
    19981 gcaaccccgg atggaccgcg acaagggcgg cggcatgacc gtcaccgtcg ggcgcatccg
    20041 caaggacccg attctggaaa acgggatcaa gtatgtctgc ctggcgcaca acaccaagct
    20101 gggcgcggcc aagggcgcgc tgcagacggc ggaatatttg gttaaaaatt atctgaaact
    20161 cgtttagatt ccaaccgggc cgcggcgggg aaatcctctc ctgccgcggc ctttttgttg
    20221 tttcgcccgc tcccggatgc ttttgaaaag tggatctccg gaaagcgggt tttttcatca
    20281 tggacctcgc cgggatatct tcttgaaaca tccggacagt tttgatacgc tccgcggcac
    20341 aaatttgtcc cgtgtacaaa tttgatgaac tcgtaaaaag taacccaaac cgtcaccccg
    20401 gcgaaagccg gggtccataa ctaattgtaa ttactggata ccggccttcg ccggtaagac
    20461 gaaaagagtg cgaaattgac tttttacgaa gccgtcaaat ttgatgaact cgtaaaagtc
    20521 cccgctgtca ccctgggcct gtcgaagggt gaacgtaagc actcgatatc acgtcatgct
    20581 tcgactggct cagcatgaca aaagagaggc aaaatgactt tttacgagac cgccgaattt
    20641 gtactttcac ggtttaaacg gaaaggatga ttgatatgca attttctgag cgcgtttttt
    20701 gcggcgaact ttcttctgaa gacatcggcc gccgggtact cctggccggc tgggtggacg
    20761 ctttccgcga tcacggcggc cttttgttta tccacctgcg cgaccgcagc ggcattgtac
    20821 agatcgtctt cagcccggac accgccacgg gcgacatctg ccgtcaggcg gccctgctgc
    20881 gttccgaata ctgtattgcg gtttcgggcg acgttcgaaa acgcctcgcg gggacggaaa
    20941 accccaacat cgaaacggga ccaatcgaag tgttcgtgac ggagctcgcg gtgcttgccg
    21001 aatcggacgc cctgcccttc tccgtttccg acaaggcaat ggtggcgggc gctccgtccg
    21061 ccggcgccga ccacgtggcc gaggacctgc gccttcagta ccggtatctg gacatccgcc
    21121 gccccgccat gcgcgacaac ctgatggcgc ggcaccgcat tttccggtgc gtccgggaat
    21181 ttctggattc ccggggcttc gtggaaattg aaacgccggt gctcacggcc agcacgcccg
    21241 aaggcgctcg cgactacctg gtgcccagcc ggatccatcc tcagaatttc tacgccctgc
    21301 cccagtcgcc gcagctcttc aagcagctat tgatgatcgg cggcatggag cgctactttc
    21361 agctcgcgcg ctgcttccgc gacgaagacc tgcgccccaa ccgccagccg gaattcacac
    21421 agctcgacat cgaagcgtcc ttcatcgatg aagagttcct ctacgaactg attgaggagc
    21481 tgaccgtccg gatgtttgcg atcggcggca tcgacctgcc gcggcccttc gcgcgcatgt
    21541 cctacatgga agcgatggac acgaccggct ccgaccggcc cgacctgcgc ttagggcttc
    21601 gcttcgttga cgtgacggac gtcttttccg aaacccgtta ttcgattttc cggcagatcc
    21661 tccagcgcgg cggctgcatc aagggcatca acattcaggg ccagtcggaa aaactcagca
    21721 agaacgtttt gcaaaacgaa tacgccaggg agatcgcgcc gtcgttcggc gccaagggca
    21781 tgacctggat gcgcgccgaa ggcggaaagc ttgaatccaa catcgtccag tttttcagcg
    21841 agcaggagct ggctgaattg aagcgccgct ttgcggttac cgacggggac gtgctcattc
    21901 tgatcgccga tccttcctac gcggtggtga cctccgcgct gggccagctt cgtctgcacc
    21961 tggccgatcg tcttggcctg attcccgaaa acagctttta cccggtctgg atcacggagt
    22021 ttccgctctt cgagcccacg gatgaagggg gcgtcacctc gagccaccat ccgttcaccg
    22081 cgccggaccg cgaggatttc gatccgaaaa atctggaaga gctgctttcg ctcaagtccc
    22141 gcgcctacga cctggtcatg aacggcgagg agctgggcgg cggcagcatc cgcatcaaca
    22201 accgcgacct gcagcgcaag atttttgccg cgctggggct ttccgaggaa gagacccggg
    22261 agaaattcgg ctttttcctg cgcgccttcg atttcggcgc accgccgcac ggagggctgg
    22321 cgctgggcat ggaccgcacc gtatcgatga ttctgcagac gccttccatc cgcgaagtga
    22381 tcgcatttcc gaaaaaccgc agcgcggcct gtcctctgac cggcgcgccc tcggcggtca
    22441 agcgcgagca gctcgccgag ctggggcttt tgaatccggg cggccaggat gtgctgccgg
    22501 gaacggccga aaaggaagac ccgatcgatc acctctcctg ggtgtcgcgc atcggcatct
    22561 ccgcggcgga gcggcccgtg atggaggaga ttctggccgg cgccgaagcg ctggcccgcc
    22621 tggcaaaaga aaaggcgggg tcggaagaac cgattcgcac ggtggcgccc gtgtccaacc
    22681 ggatgcggcc gggaacccgg gagatgcgct ctaagcttgc cgaaagcggc aaactgctca
    22741 agagcgcgcc ctcggtaaaa ggcaattatt ttaaagtcgc cagcattctg gaataaaagg
    22801 gagactcatc catggattct atttttttct accgcaatcc cgacagcgcc gcatccggcg
    22861 acggggcgct tgcgggactg aacatcgcaa ttcaacccaa tatctcaaag gcgggctggc
    22921 cggttgatgc gggctcccgg gcgctggcca atttcacggc gctcgaagac gcaaccgttg
    22981 ttgaccgcct ttcccggtcc ggcgcatttt tatgcggctc gacaaaaaca agtgaattcg
    23041 gattcggcct ggcggaaagc cgcgcgggcg aagccgtaaa gcaaaagatt gccgatacgg
    23101 aactggtttt ggacatgacg ggagaggccc gcctggctgc cgcggcggcg ggcgtctgcg
    23161 gcttcaagcc cagctacggt ctggtgtcgc acttcggcct gatcggcctg attccctcga
    23221 tggaaacctg cggcgttctt tcctcatcga tccagaatat tcgcggaata ttgaaagccg
    23281 tcgcaggtcc cgatgaaaaa gatttttcgc tgcccgatga aagcgttccg gattttacgg
    23341 cgcagctcat caacccgaca aaaactgcga tcggcgtgcc ggccgaagcg caaagcgacc
    23401 tgacggacga acagcgggaa agattccgcc tttcgattga ggaactttcg caagcaggct
    23461 ttgccgtccg ggaggtgtct tttcctgaat ggccgctgtt tttggcggtt cacaaaatcg
    23521 tcggctccgt ggaagcctcg tccgccgcgg gccgctacga ttcggtccgc tacggcgaac
    23581 gcgcgccggg cgccaaaaac tggaacgaga tgtatctggc ctcccgtggc gcgtctttcg
    23641 ggacactttt gaagagcttt ctgtttcagg gcgcgttttt ccagtttcag cgctacacgg
    23701 cgtatgaaga cgcctgccgc atccgggcgc gcctggtcgc ggatatgaaa aacctgaccg
    23761 gtgaggtgga ttttctggcg cttccggttt ccggcggcgc gtccgaccca aatccggcga
    23821 cgctcgacga aacctaccgg caattcgcct gcacggcgtt tgccaacgtc acgggccagc
    23881 cggcgctggt cctgccgccc gcctccaaag ggcaggcgcc gctgcagctt gcgggcccaa
    23941 ggcttagcga cgcggggctg ctttccctgg gcgaacacct gctgaaactg cgcgagggag
    24001 gaaaatagcc atggaatttg aagcggtcat cggattggaa atccacgttg aactcaactg
    24061 cgcgaccaaa atgttttgcg attgtcccaa caatcccggc gacgatccca acacgaacat
    24121 ctgcccgacc tgcctgtggc tgccgggcgc gattccccgg ttcagcgccg aagcgctgga
    24181 aaaggcgtcg cttctgtgtc tggggctcgg cgccgaactc cagccgaaaa gcgccttcga
    24241 ccagaaggtt tactactatc ccgatcttcc gaaaggctat cagctttcac aggcccacct
    24301 cccgctggcg cgcggcggcg gcatcgacat ttccgatgaa gacggcaaga cgaaaagact
    24361 gcgcattcac cacatccaca tggaagaaga cgtcgccaag ctggtgcacg agatggaggg
    24421 gcgtctgccg atcagcctgg tggacttcaa ccgcgccggc tccccgctgg tggaaatcgt
    24481 gacggagccg gatttccgca cgccgcacca cgccatggaa tttctcaagg cgctgcgcac
    24541 gcaggtgcgc tatgtcgggt cttccgaatg cagcatggaa aacggcacga tgcgcgtgga
    24601 tgccaatatt tccgtgcgtc ccaaaggcac ccgccagatg aacacgaaag tggaagtcaa
    24661 aaacatgaac tccatccgcc acgtgggcga tgcgatcgct tacgagatca gccgccagag
    24721 tcaggcggtg caggcgggcg aggcggtggt cctgcacacc cgcctgtggg atccggaaaa
    24781 gaaagtcacg ctcccgatgc gcggcaagtt tgaaggcccg tgcgtgcccg acccgatggt
    24841 gccggtcatc gaactgacgc cggaatggat tgaaaaaatg cgtgtgcgtc ttccggaaat
    24901 gccggccgca cgcgccgggc gcttcgtgat gcagcacggt cttaccggcg aggaagccgc
    24961 gtttctgagc gaagatcccg aagtcgccgt gtatttcgaa gcgctggtcg tccggggaat
    25021 cgcgccgcgc acagccatgc actggctggc cacacagctg cttccggccg tcaaagaacg
    25081 aaaaatggag ctcgcctcgc agcccgtcac gccggagcgc ttcgcggcgc ttctgaagat
    25141 gctggccggc gatgaaatca acgccaacgc ggcgcgcgaa gtgctgacgc atctgtttga
    25201 aagcggcgag tcgccggagg aaatcgtgaa ggcgcgcggc ttcaaacagg tgtccgacgc
    25261 gggcgcgctc gaagccatga tcgatcaggt gctcgcgacg caggccgcgg ccgtggccga
    25321 tttccgcgcc ggtcagggaa aggcgctggg ctttctgatc ggccaggtga tgcaggcatc
    25381 cggcggcaaa gccaacccga aaatcatccg ggagctgctt gccaaaaagc tgaacgcggt
    25441 tccataaaaa aacagggaac ggttacaaac cgttccctgc aacttctttg tcatccccgg
    25501 cctgatcggg gatccagtag cgcgcagctt tagcggcggt gtttttgcaa aaaatagaac
    25561 cgtccccttt ttctgcgtca gccttttttc aagccgccgg cgattttggc ccaggaatcc
    25621 cgcaggggaa ccacacggtt gaagacgggc cgccctgccg ccgaatccgg atcggcgcag
    25681 aaatacccca gccgttcaaa ctggtactgc gccccgggac gcgcgtcttt gaggctggcc
    25741 tccgccaagc aggccgaaag tgtttccagg gaattcggat tcagacagct caaaaaatcc
    25801 tcttcactgc ccggatcttc gatcgtgaac agacggtcgt acaggcgaac ctcggcgggc
    25861 acggcatgaa ccgccgacac ccagtggatc acaccctgca ccttgcgtcc gtccgccggc
    25921 ggcgcgctgc gggtcgccgg atcataggaa cagtggattt ccgtcacctc gccggtcttt
    25981 tcatctttgc gcatgctttc aaatttgatg atgtaggcgt tgcgcaggcg aacttcccgt
    26041 cctggtccta gacggttgta ctttttgggg ggatcttcca gaaagtcatc gcgctcgatg
    26101 tacagctccc gtgaaaagga aacctttctt gttcccatct caggatgctg cggatggttg
    26161 gcgcagtcga agtcctccgt ttgctgttcc gggtaattgt cgatgatgac tttgagcggc
    26221 cgcagaatgc acatggcccg gggcgctttt tcgttgaggt cttcgcggac gcagtgttcc
    26281 aagagcgaaa catcgatcag gttgtcgttt ttggccacgc cgatctgggc gcagaaattc
    26341 cggatcgcct ctggcgtgta gccccgccgc cgcatccccg tcacggtggg catgcgcgga
    26401 tcatcccagc ccttcacatt cctgtttctc accaggtcga taagcctgcg cttgctcaac
    26461 acggtgtagc tcaggttgag ccgggcgaat tcaatctgcc gcggccggtt gccggcaatc
    26521 agtttctcga cgatccagtc gtagagcggg cggttgtttt cgaactccag cgtgcagatg
    26581 gaatgcgtga tgccctccag cgcgtcggag aggcaatggg cgaagtcgta catcggatag
    26641 atcacccacc ggtcgcccgt ccggtagtgc ggttcgcgct tgatccggta taaaaccgga
    26701 tcccgcatga cgatgttggg cgaagccatg tcgattttgg cgcgcagcgt gtgcgaaccg
    26761 tccgggaatt cgccggcccg cattcgctca aacaggctca ggttttcctc caccgaccgg
    26821 ttgcgatagg ggctttccct ccccggctcg gtaaacgtgc ctcggagttc gcgcacctcg
    26881 tcggagttga gatcacacac ataagcgtcg ccttttttga tgagctccac ggcaaacgcg
    26941 tagagttttt caaaatagtc ggaggcgtaa aacagacggt cgccccagtc aaatcccagc
    27001 cagcggacat cgtggataat ggattcgacg tattccatgg actcgcccgc cgggtccgtg
    27061 tcatcgagtc tcagattgca ggttcccgaa tactgtagcg cgatgccgaa gttaaggcat
    27121 accgatttgg catgcccgat atggatatag ccgttgggtt cgggcggaaa gcgggtcgcc
    27181 acgcgccctt cgtgtttgcc gg
//
LOCUS       DAOSRM010000019        26920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_1989_length_26920_cov_105.233389, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000019 DAOSRM010000000
VERSION     DAOSRM010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 26920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26920
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_26920_cov_105.233389"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..754
                     /locus_tag="PLA58_03390"
     CDS             227..754
                     /locus_tag="PLA58_03390"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOR61871.1"
                     /translation="MGNVKIALRPSSISDASLRYHWFNDAEFTRLYLGRPLFTSYRQV
                     EDEILLSTYPTLSTGLYELAIQTVETNLYIGNTFFRKMDWQNRSAEYGIFIGDSERRG
                     AGIGPEVTQRMLEQGFSEFGLHRIWLTVLAFNERAIRCFEKCGFQREGVLRDAIFSGG
                     QFNDVMMMSVIAGAN"
     gene            810..3650
                     /locus_tag="PLA58_03395"
     CDS             810..3650
                     /locus_tag="PLA58_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR61872.1"
                     /translation="MKSKCLRISLFAALFAAAFVFAASIPVRAAWFSKGGKDYYQEGL
                     NHFQKKNYYLSMQSLEKALQLKPDYPEALGVLGWDYIKVGRALDAEAIFTKKYEKNNA
                     DISAIQGLAWAKFALGKNEESEKYFNMELKWANDFITKDNWIFFGPSDKEYIESIYSD
                     ANYGLASLAGAQKNYALAAKYFETALQYPNSFTSRTDLLTACGDIYYYQAKYAEAIPV
                     YERAVKEDQKNLSAQLKAAWSYYFNNQFAAAGAAFEKALAVSSRQESVEALYGIALTD
                     YRQNKFDQAYRNLAKAIAVNPYYMDNAVVHDMIAKKPEWKTLWKNFGLAYTASNYYAA
                     VYKLDGYLQVVKADDVEALLADGWSYLGLGYLDKALQLFEAALKFNPKSDEAYVGLGS
                     SYLAYKKPAEALKAYQQALAINPQSALACNGLAYYYLSQKDEVKALESAQKSVSLKSD
                     YYDSQAFVGNALFRQKKYDQAIKEYDKLIKIDPSVFSSWNLLGWANYSAGKYDAAVKA
                     FAESKRINPYLVEAHYGLGMCYARTGDMNDAKDEFSAAINLYPYYSHTKDLVSLIKAN
                     PKWNDLYKTLGWSYYNYQQYSLAASAFKEYLAAEPGDIGALRGMAWSNYWLGQLDTAY
                     AGFRGILKEEANDVDALVGTGWILYIRGKDGEAMGYLQKAVKINDKALNAWRTVAAIH
                     FRAKRFEEASAIYKKIADLQPYAVDTYNNQGWALYKENKFKEAVAKFNESLRVNRYLG
                     EPYYGLGLSYAKLGDIEKARENFASAVYLYPAYMDGQDLYAVFDSNPKLKDLYSALGW
                     GYYYQYYYDKAKFHFNKALKADAGNSDALLGVGTVAFILGDFNGAIEAYKKLLPNVPA
                     KASFWDKYSYMLDNLGWSYYYQKQYDSALETFKRLEAYHPGISYIAPINGKGWCELKK
                     GNKDEAQKLFQQSLKIVPYNYTAEAGMKEMKK"
     gene            3652..4059
                     /locus_tag="PLA58_03400"
     CDS             3652..4059
                     /locus_tag="PLA58_03400"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOR61873.1"
                     /translation="MKSLRKSQGKTIRCVLAGGQTKSCPPVKDEGLPLCEKHKRELNR
                     SARCRIADDIRGNFMEKGDESIVFNPKDGGVYSLNGTGTFIFKEIMAGKTFCEILAKS
                     QSMFDAENVSEMIADYRLFIEELKKSNVITAEA"
     gene            4061..5110
                     /locus_tag="PLA58_03405"
     CDS             4061..5110
                     /locus_tag="PLA58_03405"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOR61874.1"
                     /translation="MLQAHVVITGLMATDNPYPGLGIARCLRDAPEFRGRISGFVFDP
                     LSTGAFCEGVFERVFLVPYPAAGVEVLLERIGEIHRADPIDVLIPSLDSEVILYAQAA
                     RELATLGIRTLLPPVNSIKMRSKNQLYEFCRQHRFRCPETVILNNVQEIAARCEALGG
                     TPFVLKGALSDAAVCHSIEEGEYQYGQLFNQWGYPILMQQYIAGEEIDVIALADKKSR
                     LSGAVVMKKFGLTPKGKAFSGITVEDDKPVQLTRQILKSLKWIGPAECEFLKDEKGHY
                     FLMEINSRFPSWLYLAAAAGQNLPLLTVQLACDMPVRPLTSYTAGKLFVRTVADALLD
                     ARQIMELTASGEVRL"
     gene            5107..6051
                     /locus_tag="PLA58_03410"
     CDS             5107..6051
                     /locus_tag="PLA58_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61875.1"
                     /translation="MIIAVTGLNTAAEPEAGFGVLQSLSGGGAGHDRLIGFAYQPLDA
                     GLFHSGLMHRAYVVPPPKTDRMAFLKRITLVKKKRGLDMLIPALDDEMELFVDMAPQL
                     RAAGIAVLLPSGKSLRELQSEMYRQIGKQQAAAGNDEQDDPPVLPPALVKNPVSEQTA
                     MDAAVPDYFSVAVVANSQSRIVAIASVRKLLISRYGGTWMALTVDNHEFLGVAEEVVT
                     STAWQGALTIDVARNRQGEQYLSGFHPTFPDWIGLARGAGANLPAVLIDLIKKKRIRG
                     VAQTAPGKVWIRSSIDLVTDLNHFGDISLNGEMTYDQK"
     gene            6038..7417
                     /locus_tag="PLA58_03415"
     CDS             6038..7417
                     /locus_tag="PLA58_03415"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOR61876.1"
                     /translation="MIKNKGTKPYVRPVIKKISFGDTVKFKNEHMKTPAVKALEGLSV
                     SDLTDRFGSPLYVVSEGTLRRSFREVAQAFTRRYPRTIIAYSYKTNYLSGICALFHSE
                     GAWAEVVSGFEYEIARRLGMDGPSILFNGPYKKPEEMKRAFLEGASVHLDSFDELKMA
                     GEVAASIGRKVNVGVRVNMQLNYPAWDKFGFSYERGEAFEACRQIKKHRYLQLSGLHC
                     HAGTYIIDLGIYRRVIENLTDLSLDIQKELGREVEYLDIGGGYPSGNSLHTQLMPGYT
                     IVPPMEQYAETVCSVLNGKLRSFTKAPRLILEPGRSVVDEGMHLLTKVVSVKERQNGN
                     RAVIVDAGIHLLPVSAYYRYEMSSNRDSTPTTEDVDVYGALCMQIDVLRKSVRLPVAQ
                     AGDILTIHKVGAYNFTQSMQFIYPRPAVVLLHDGRAEILRRRETYEDIKGPEAIPARL
                     LTYAAKPKR"
     gene            7395..9311
                     /locus_tag="PLA58_03420"
     CDS             7395..9311
                     /locus_tag="PLA58_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOR61877.1"
                     /translation="MLLNLRDKLQGVLEEAAGWIKAPDSGPNALQAAVIVLRSYGQVV
                     FAAHAVSGLFLLIALATISRSTVWFSLVGIAAASLSAYAFRAERSFIRQGIFGFNGAL
                     LGIFWSWYFTVSIVSVLLLVVMAVIVYLVQSFLMNKLSLGRFNLPVMSLPSVIVLIAS
                     LLIVYWLVYNAWLIPASAIYRMGGYAAEPLIPMEGVLKSLLPGLPLPRHVVAWLLIFT
                     GILICSRISAFAAALGALSGYAITRMLPATTGVLYTSDLFIGFNAIPVSIGLLGVFLV
                     ANGPAFFFTFAGLVVCSVFWLLLIQLFGLLNFPFLTLPFNLTILAVLITLKKMNSPPA
                     GLYPVPLEVITTPEQIILWHKNNILPRAAVKTPETVAQLLLHPSRLFRPSREEINAVQ
                     VLVDGAEKIAVLSGAGTSTESGIPDFRGHYNFWRKFGAEDFTYQNFLNREDVRARYWA
                     MERQFYAMILRARVNPTHQAVKWLDDQNRLSCIVTQNVDGLFQKAGVNPEKVIEIHGT
                     EHQVRCLNCGAIGNRQDIETMFPAGIYIPYCYACYGWLKPATVLMGEELDKVLFESAL
                     SKILSSDLLIIMGTSLQVDPVASIPALAWKKGIPIIIINSTPTPQNHLSKLFIHCPTG
                     KFFRKFLRQVEKRR"
     gene            complement(9501..9989)
                     /locus_tag="PLA58_03425"
     CDS             complement(9501..9989)
                     /locus_tag="PLA58_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61878.1"
                     /translation="MLKEHQEKILELLEQLELNMCDLYLLFADRFPKYADLLTTVARQ
                     ETDHADGIKKLHLLAGDDRVLFDEKITKTYTVKMFISSIQEALARAKSGKMSLINALS
                     VSHDFEQAILEKGFYNFFSGQDDETRAVIRKITEETLDHQMKMKKAWEQERKPTPSQA
                     AS"
     gene            complement(10309..11940)
                     /locus_tag="PLA58_03430"
     CDS             complement(10309..11940)
                     /locus_tag="PLA58_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR61879.1"
                     /translation="MKKQNFIRKVFLSMILPIILIFNAGLYAAENPAAPKPDYDVIVI
                     GAGMGGLSAAAHLAVKGQKVLVLEKHFVVGGSTSKFTRGEFVFDVALHEMAGGGPGKM
                     DRGLYQLLKATGVDKKVELYEVPHFYRSVFPGVDITLPPNWPGFKSELKKKWPEESEG
                     IEKFHVLCADLFKDLMELRDLFRKGSVSGAFTQILVPFSQPTFFKWKDRTVKDLMDHC
                     FKNEDLKAVVSQLWVFYGASVDKESALIFMAATESYLSDGAWHIKGTSQALADGYADR
                     IRELGGTVKTGSLVTKILMENGRATGVRTADGKTYTARYIVANTDPYQLADKLIGREN
                     LPAKYLAKIDAMKPANSLFGVYIGLNIDLKALGYNDTEIFYNTYRESMKNYEAMMKGD
                     FDKGAVAITIYSNLGDPVYAPKGKSVVKLDAYSDISVWPKDRAEYNKLKAQKVDELIA
                     LAARVIPELKDPKNIIVKEGYTPRTIEHFTMNKGGVVYGFDLTPDQWEKVPNTTPVPN
                     VFIASNWTQAWHGVGSCQINGWRAARLILDREGIE"
     gene            complement(12130..13545)
                     /locus_tag="PLA58_03435"
     CDS             complement(12130..13545)
                     /locus_tag="PLA58_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOR61880.1"
                     /translation="MSLSEELKYCDATALAGLIRKKEITPLELIESAIEKIEKLNPAL
                     NAVVTPMYDLARQAAKLKRLKGPFAGVPFLLKDILASYGGVRMTLGAKVLKDLVPDHD
                     SELVLRHKKAGLIVIGKTNTPEFGILPTTEPELFGPARNPWNTNHSTGGSSGGSAAAV
                     AARIVPMAHGNDGGGSIRIPASCCGVFGLKPTRGRITLGPDFGDMIGGLVNEHALTIS
                     VRDSAALLDATAGCLRGDPYWAPPVKRSFLKETETDPGRLRIAFTAKAPTDKPVHEDC
                     VRAVEEAARLCEELGHSVEESAPVIDGKTLTRAFVVLWFAGIASIVKMLGVSKEQVEP
                     LTWAIYEQSQTASAADYLIAVSTIQRVARDVASFFEKYDILLTPTLAKPPAPLGSFDP
                     KPDNPLAGFAESARYAVFTPICNATGQPAMSVPLFWNSEGLPVGAHFIGRFGDEATLF
                     RLAGQLEKARPWKDRRPPVCA"
     gene            complement(13615..13920)
                     /locus_tag="PLA58_03440"
     CDS             complement(13615..13920)
                     /locus_tag="PLA58_03440"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOR61881.1"
                     /translation="MNFRKTWIPVVCATVLSGAVFASVVTAAGPPLKAKTCAACHNDY
                     AKILPKEHIPVDKTAGCMSCHASKPGSDEPTRFSTNAHKIHQGEKTSVECGACHDLQ"
     gene            14301..15137
                     /locus_tag="PLA58_03445"
     CDS             14301..15137
                     /locus_tag="PLA58_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOR61882.1"
                     /translation="MVLGLSLLIVAGSYRRLGNCEMFAKDVALRAGADSIFLLRLTDF
                     ELKPCTGCHRCLNPEHRCRVQDDLCFILEKMKGFDGIIFSIPTYVLGPVGQFKLFADR
                     LSSMAVFHPDFRNIRAVSAIFGGIESWRGVTQSLVNAVIRMMGFDFRGSAFVEAALPG
                     ESLEAKYQPVAQGLADVLSKRIETYFPCKTLRCPSCGADLFFLEQGRRVCALCGSSGE
                     EQDGVWLHVEDSGRFTTKGFVEHFESWLKPKVLEYAAQREHHRALRETYKNIGTWIKK
                     EE"
     gene            complement(15320..16024)
                     /locus_tag="PLA58_03450"
     CDS             complement(15320..16024)
                     /locus_tag="PLA58_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOR61883.1"
                     /translation="MTGKKEKEIRDLLVKALQKGQLALSEYESRKVIASAGVPIPREA
                     LVQSRDEAMDQAEKIGFPVVLKGSSPELTHKTEMGMVRVNLKNRDDVGGAFDELSGKG
                     IRLEGFLVQEMVEGRREFVIGLTRDPQFGPSVMFGLGGIFTEAMKDVSFRVAPLSEDD
                     AREMIGEIRAAKLLDAFRGEKAVDREILVRALVGIGHLGMKHEEIAEIDINPLIIRDG
                     KPIAVDALVILKAHRA"
     gene            complement(16021..17460)
                     /locus_tag="PLA58_03455"
     CDS             complement(16021..17460)
                     /locus_tag="PLA58_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR61884.1"
                     /translation="MELFFDPRSVVVIGASNTPFNLGHTICNMLKDYLHYQGAVYAVN
                     SKGEAVNGCPGYSSVLDLPEAPDLAIIIVAAHHVPGLIEDIARKGIRRVVIESAGFSE
                     GGEIGEAMQREIDTIARQNGIRILGPNCLGALSTRDKFCCFYGVNPSLVEMNQIFESP
                     GNISYIIQSGGVAVLVMESLYYDIVGVNKVVSIGNKCDVDEADLIEYFQKDETEVIGL
                     YLENISNGRRLMEAARKSHKPVLLYKVGKTKEGAMAAMSHTAGMANNDRVFDAACRQT
                     GIIRLQSIDELHSLPKMFTEMPLLKGKRIAAFTNSGAFGGISADLLVQAGLQMVRLAP
                     QTQEKLKKVGQVFNVKNPVDIGPALPQTYLDIFEILLSADEVDGLLPLLSVWQPFVID
                     CLLELMKMCKQYEKPAAIYTPNAASKSIAIRKKSRIPIFETTEQAVRALAVSQMYWAS
                     LLKKNFTGEKTSCSAGMKANHRSTGEAMQ"
     gene            complement(17578..18099)
                     /locus_tag="PLA58_03460"
     CDS             complement(17578..18099)
                     /locus_tag="PLA58_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOR61885.1"
                     /translation="MEGWTEEEIRDRRLMAPCGLYCGACGVYIATRDHNEKFREVMGN
                     LYGTKKEDTQCLGCMQSDPPEKLYGYCMNCSIRTCVRKKGFYSCHQCDQWPCGLIENF
                     GLATGLRVMKRAIPLWREKTAALGDEQGSEEWARAELERYHCPACGKPLFRGAKRCRA
                     CKAEVADALDGTL"
     gene            complement(18177..18956)
                     /locus_tag="PLA58_03465"
     CDS             complement(18177..18956)
                     /locus_tag="PLA58_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61886.1"
                     /translation="MKSHGRKIVLALTLIFAMLIVAEANAFEIGARGYVWFPDLKKSD
                     IQTIADGIEGTDIDARDMLGVGNTATFSVEAYGGVGKNHISLMFTPFGYTTDNVLAAA
                     LNYNGINYTAGSAVHSDLSYSMFDLRYQRDLINMENILAGFSLGGIAQIKYSTGSFKL
                     NSAGKVEQSNSFNSVIPMIGLGAHIGILANLLEVRAQATGGGYGSDNYSYEAMADLSL
                     TPFPFLDIHAGYRLLQQKMDVNDYKMDTLYTGPYIGLSLGF"
     gene            complement(19065..21143)
                     /locus_tag="PLA58_03470"
     CDS             complement(19065..21143)
                     /locus_tag="PLA58_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOR61887.1"
                     /translation="MKKNLKILNEFEGASLLRQSGIPVIDGILAHTDAEAAEAAGRLG
                     FPVALKICSEAVPHKTERGGVSLNIKDTAALERAVREMKKRFPDIPFALLVQKMARPG
                     TELILGCRRDPVFGPIVLAGIGGIFTEIFRDSVIELAPVDEEDAPAMLRRLKGVALLD
                     GYRSREPLDVAAIAKSLSALSRLIADRPDIAEIDINPFIAYPQGAVAVDALVRLDGAS
                     PAPARKRPSPATIAPFFQPSSMAVIGASRSPGKGGNIILRNLQKAGFKGALYPINPTV
                     KEILGLPSYARVSDVPGKVETAMVVIPKTAVPDALEDCAAKGVTNIILSTGGYSDAGE
                     EGAREQKTIIEMARRAGIRIMGPNSIGTLNPSAGMSTSIVGLDPIRKGGVSIIGQSGV
                     FSSGWARWIADTKPFGLAKVACIGNKGDVNESDLLEYLTGDSATRTVGMYLEGVVEGK
                     RFIRAAAAACKAKPVVVMKAGRSEAGAAAVASHTGSLAGSDAVFDAVCRRTGLVRTQN
                     SEAFFDALAAFEKLPLPRGNRMGALSITGMGCVAATDAVEECGIDLPALQPDTLRKLR
                     EVMPVWAPVRNPIDIWSAIEQHGSKKATSHIARCLMEQPDVDALLITFVLMSESMFDI
                     PEAFADIFRRHPHKPVFASYYGGTAREIAHIHEGFAALGVPCYPTPERAIFAFSRMVE
                     YARFRGVIGK"
     gene            21224..21502
                     /locus_tag="PLA58_03475"
     CDS             21224..21502
                     /locus_tag="PLA58_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61888.1"
                     /translation="MHIAADPSVDLLIDSREENARGGRTAARALTVTGTIRRGLDEPE
                     KNTVRAVLCRRHPDLKDFFSDPSVEVIVVKINTLQLLNGITDSYFEEV"
     gene            21729..23522
                     /locus_tag="PLA58_03480"
     CDS             21729..23522
                     /locus_tag="PLA58_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOR61889.1"
                     /translation="MYQLVKPDNIVEILEKTVAKFPERPFLGTKNKALKQYDWISYAD
                     FGKRVDHARSGLSQLGIQKDDAVGIICNNSTDWAVCFFAVSGLDARFVPMYEAELASV
                     WKYIIQDANVKLLFISKKAIYEKIKDALAAMPSLQKIIIMEGEGPDTLAELEKKGAGL
                     KVKSIYPAPSAICILIYTSGTTGDPKGVLLSQANITSNAHAVTVCFPDINENDNSLSI
                     LPWAHVFGLGELIVYCLLGASMGLAESAATIPEDLALVKPTFMTAVPRVFNKVYDGLW
                     TKMNEEGGLAKTLFVMGVESGKKKRELAAQGKSSFMTNLKFKIADAVVFQKVREKLGG
                     RLKGVMTGSAMMNPEISHFFWDMGVPLYDAYGLTETSPGATMNCPAKYKIGSVGPPMG
                     LGKIVIDKSVVGPDATDGEIIIYGPNVMQGYHNKPEATKAVMTPDGGFRTGDRGRLDE
                     EGFLFITGRLKEQFKLENGKYVFPAAIEEDMKLNHYIENAMIYGEGREFNIAIVIPDF
                     AALDKWAAKNNLPKDHAELLKRDETKKFLESEILNALKSTYGSYEIPRKFVFIEEPFS
                     LENGMLTQTMKLKRRVVFDKYKDQIEKLYAK"
     gene            complement(23639..24739)
                     /gene="mutY"
                     /locus_tag="PLA58_03485"
     CDS             complement(23639..24739)
                     /gene="mutY"
                     /locus_tag="PLA58_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOR61890.1"
                     /translation="MSKGQAEIKTFFRSRLLAWYKRHGRLLPWRETTDPYRIWISEIM
                     LQQTRVDTVIPYYKRFLKRFPDVFALARAPLQDVLKTWENLGYYSRARHLHAAAGIIV
                     GRFGGRIPDTPEDIRTLPGIGDYTAGAILSIAYGRAVPAVDGNVRRILSRVFAIDRPV
                     NDPRGQKHFFELAASLVPGKRASDFNQALMDLGATICRAKNPDCGVCPLASICLARRG
                     GLQNDLPVSRKSPAIPHRQAVAAVVRNGKGLLLVVQRQAEGLLASLWKLPGGFVQDDE
                     NREAGLRRCVKEELGLSIRVGKKIASADHAYTHFRVTLHVYQGFPLSGKPTARGCQDW
                     RWAAAADLKKLPLSRIDRMAVALVEISPVRFP"
     gene            complement(24751..25383)
                     /gene="lepB"
                     /locus_tag="PLA58_03490"
     CDS             complement(24751..25383)
                     /gene="lepB"
                     /locus_tag="PLA58_03490"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOR61891.1"
                     /translation="MKENKSKPAPAKSKIREFIEAILTALLIAAFIISFIVQAFKIPS
                     GSMIPTLLVGDHLFVNKFIYGVKLPYFRATIIPVTDPKREDVVVFIYPEDRSKDFIKR
                     VIGVAGDTIEMKNKKLFINNQPYADPYGVYDDHTMLPSDVQPRDNFGPVVVPEKSLFV
                     MGDNRDHSLDSRFWGFVDLKDVQGKAFILYWSWNAEDKTVRWSRIAKLIR"
     gene            complement(25435..25995)
                     /locus_tag="PLA58_03495"
     CDS             complement(25435..25995)
                     /locus_tag="PLA58_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOR61892.1"
                     /translation="MDSKNLFLLPVMISLIGHAALITASSMVDLRNQEKTAQPFTVHI
                     AGPPPPVTPEKDGDATDKKVSEDSRDAKPAPAGNREETVNIGSSDVKYAAYLSGVKQK
                     ILRIWKYPEAAYQMEEEGVVVVKMSIDANGRLSQVALMNSSGFIHLDSSTLDVVRAAE
                     PYQPLPRQYDLSRLHIIASFNYRIEK"
     gene            26088..26561
                     /gene="tadA"
                     /locus_tag="PLA58_03500"
     CDS             26088..26561
                     /gene="tadA"
                     /locus_tag="PLA58_03500"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOR61893.1"
                     /translation="MLTDEDYMRMALDEARKALGRNEVPVGAVLVQDDRLLVMAHNAP
                     ISGVDATAHAEMTVIREACRMSGNYRLTGARLYVTLEPCLMCAGAILQARLARVVFGA
                     RDPKAGAVFSLFQVLQDSRLNHRVEVTEGILREECGEILSRFFKEKRVEAGSVSP"
     gene            26599..26692
                     /locus_tag="PLA58_03505"
     tRNA            26599..26692
                     /locus_tag="PLA58_03505"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26633..26635,aa:Ser,seq:gga)
     gene            26766..26859
                     /locus_tag="PLA58_03510"
     tRNA            26766..26859
                     /locus_tag="PLA58_03510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26801..26803,aa:Ser,seq:cga)
BASE COUNT         5876 a         7271 c         7612 g         6161 t
ORIGIN      
        1 ggtgctcaac gaaacctatg tttaccttca gcaactcaaa cgggaaggcg cgatttggga
       61 gggcatattg gggaaaaccg ttgtttattc agtagcttga cgctcagcga gggcttttaa
      121 agttttaaaa ttatcaatgg acatttagaa tggcaaatgc taaaaaataa atcaggcatt
      181 taaatacagc gttcacctgg tcattacagg aattgttgag tctgttatgg gcaatgtgaa
      241 aattgcttta aggccatcca gtatttccga cgcatcactt cgttaccatt ggttcaacga
      301 cgcggaattc acgcggcttt atctgggccg ccccttattt acttcttaca gacaggtaga
      361 ggatgaaatc ctgctttcca cgtatccgac cctgtccacg ggattgtacg aactggccat
      421 tcaaacggtc gagaccaatc tctacatcgg caacaccttt ttccgaaaaa tggactggca
      481 gaatcgttcc gcggaatacg gaatcttcat cggagattcg gagcgccgcg gagccggcat
      541 cgggccggag gttacacaaa ggatgctgga acagggattt tccgaattcg ggctccaccg
      601 catctggttg acggttctgg ctttcaatga acgggcaata agatgttttg aaaaatgcgg
      661 ttttcagcga gagggggttc tgcgggacgc cattttttcc ggcggtcagt tcaacgacgt
      721 catgatgatg tcggtcattg ccggcgcgaa ttgatcatta tttatttaag gatattcaac
      781 tttttaaaag cgaaaagagg aggcgattta tgaagtctaa atgtttacgg atcagtttat
      841 ttgcggcgtt atttgcggca gcttttgtct ttgcggcaag catccctgta cgggctgcct
      901 ggttctccaa gggcggcaag gattattatc aggaaggctt aaatcatttc cagaagaaaa
      961 attactatct ttccatgcaa agcctggaga aggccctgca gttgaaaccc gactacccgg
     1021 aagcgctggg tgttctggga tgggactata tcaaggtagg aagggcgctg gatgcggaag
     1081 cgatttttac aaagaaatat gaaaagaata acgctgatat ttcagccatc cagggtctgg
     1141 cctgggcaaa atttgcgctc ggcaaaaacg aagaaagcga aaaatatttt aatatggaat
     1201 tgaagtgggc gaacgatttc atcacgaaag acaactggat cttcttcggt ccttccgaca
     1261 aagaatacat cgaatcgatt tattccgacg cgaattacgg cctggcgtct ttagcggggg
     1321 cgcaaaaaaa ttatgctctt gccgcgaagt attttgaaac ggcgcttcag tatccgaact
     1381 cgttcacaag ccgcaccgat cttttgacgg cctgcggcga catttattac tatcaggcca
     1441 aatacgcgga agcgattccc gtttatgaaa gagccgtcaa agaagatcaa aagaatcttt
     1501 ccgcgcaact caaagcggcc tggtcctatt acttcaacaa tcaatttgcc gccgccgggg
     1561 cggcttttga aaaagccctg gccgtgtcca gccggcagga atccgtcgaa gccctttacg
     1621 gaatagcgct taccgattac cggcaaaata aatttgatca ggcctaccgg aacctcgcca
     1681 aagcgattgc cgtcaacccc tattacatgg acaacgccgt cgttcatgac atgatcgcga
     1741 agaagcccga gtggaaaacg ctttggaaaa actttggact ggcatatacc gccagcaatt
     1801 attacgccgc tgtttacaaa ctggacgggt atctccaggt cgtgaaagcc gatgatgtcg
     1861 aagcgctgct tgcggacggc tggagttatc tcggactggg ctacctggac aaggcccttc
     1921 agctttttga agccgcgctg aaattcaatc caaaatcgga tgaagcgtat gtcgggctgg
     1981 gctcatccta tctcgcttac aaaaaaccgg cggaggcgct taaagcctac cagcaggccc
     2041 tggcgatcaa cccccaaagc gccctggcct gcaatggact ggcctattat tacctctctc
     2101 agaaagacga ggtcaaagcc ctggaatccg cgcaaaaatc cgtttccctg aagagtgatt
     2161 attatgattc gcaggcgttt gtcgggaatg ccctgttcag acaaaagaaa tacgatcagg
     2221 cgatcaagga atacgacaaa ttgatcaaga tcgatccatc cgtgttttct tcctggaacc
     2281 tgctgggatg ggccaattac tccgccggca aatatgacgc tgccgtaaag gcttttgcgg
     2341 agtccaaaag aatcaatccc tacctggtcg aagcgcatta tggattggga atgtgctatg
     2401 ccagaaccgg cgacatgaat gacgcgaagg acgaattctc cgcggccatc aacctttatc
     2461 cctactattc ccacaccaaa gacctggtaa gtctgatcaa ggccaatccc aagtggaatg
     2521 atttgtataa aacattgggc tggagctact acaactatca gcagtacagt ctcgcggcat
     2581 ccgccttcaa agaatacctc gccgctgaac ccggcgatat cggcgcgctg cgcggaatgg
     2641 cctggtccaa ctactggctg ggccagctgg atacggctta tgccggtttc aggggaatcc
     2701 tcaaagaaga ggccaacgat gttgacgcgc tggttggtac gggctggatt ctgtacatcc
     2761 gcggcaaaga cggtgaggcg atgggctacc tgcaaaaagc cgtgaagatc aacgacaagg
     2821 ccctgaacgc ctggagaacg gttgccgcca ttcacttccg cgccaaacgg ttcgaggaag
     2881 ccagcgcgat ttacaagaaa atcgccgatc tgcagcccta cgcagtggac acttacaaca
     2941 accagggctg ggcgttgtac aaggaaaaca agttcaaaga ggcggtggcc aagttcaacg
     3001 aatccctgcg cgtcaaccgg tacctgggag agccttacta cggcttgggg ctcagttatg
     3061 caaagctggg cgacatcgaa aaggccagag aaaattttgc ttctgcggtg tatctgtatc
     3121 cggcctatat ggacggccag gatctgtatg ctgttttcga ttccaatccc aagcttaagg
     3181 atctttacag cgccctgggc tggggctact attaccagta ttattatgac aaggcaaaat
     3241 tccatttcaa taaagcgttg aaggcggatg ccggcaattc ggatgcgctt ctgggcgttg
     3301 gtacggtggc ctttatcctg ggcgatttca acggcgccat cgaggcctat aaaaagctgc
     3361 tgccgaacgt tcccgcaaaa gcgtctttct gggacaaata cagctatatg ctggataatc
     3421 tgggctggag ctactattat cagaagcaat acgacagcgc cctggagact tttaagcgtc
     3481 tggaagctta tcatccgggc atttcttaca tcgctcccat caacggaaag ggctggtgcg
     3541 agctgaaaaa aggcaacaag gacgaagccc agaaactgtt ccagcagtcg ttgaagattg
     3601 tgccctacaa ctacaccgcg gaagcgggca tgaaagaaat gaaaaaatag gatgaaatct
     3661 ctgaggaagt cgcaagggaa aacgatccgc tgcgtgttgg cgggcgggca gacgaaaagc
     3721 tgcccgcccg tgaaggatga gggactcccc ctgtgcgaaa aacacaaacg agagttgaac
     3781 cggtcggccc gctgccgcat tgccgatgac attcgcggca attttatgga aaagggcgac
     3841 gagtccatcg tcttcaatcc gaaagacgga ggggtgtatt ccctgaacgg caccggcacc
     3901 ttcatcttca aggaaatcat ggccggcaag acgttctgtg aaatccttgc caaatcccaa
     3961 agcatgtttg atgcggaaaa tgtttctgaa atgatcgcgg attaccgtct gttcatcgaa
     4021 gagctgaaga aatcgaacgt tatcacagcg gaagcttaac atgctccagg ctcatgtcgt
     4081 catcaccggc ctcatggcta ccgataatcc ttatcccggc ttaggaatcg cccgctgtct
     4141 gcgcgacgcg cccgagttcc gcggcagaat ttccggattt gtcttcgatc ccctttcgac
     4201 gggcgctttc tgtgaaggcg tgtttgaacg cgttttttta gtcccctatc ccgcggccgg
     4261 cgtggaggtt cttctggagc gcattggtga aatccatcgt gcggatccta ttgatgtcct
     4321 gattccgtcg ctggattccg aagtgattct ctatgcccag gccgcccggg agcttgcgac
     4381 gctgggcatt cgaacgcttc tgccgccggt caacagcatc aagatgagaa gcaaaaacca
     4441 gctttacgaa ttttgccggc agcatcggtt ccggtgcccg gaaaccgtca ttctcaacaa
     4501 cgtccaggaa atcgccgcgc ggtgcgaggc cctgggaggg acgccgtttg tgctgaaggg
     4561 cgctttaagc gacgccgcgg tgtgccattc gatcgaggag ggtgaatacc agtacgggca
     4621 gctcttcaac cagtggggct acccgatttt aatgcagcag tacatcgccg gagaagaaat
     4681 cgacgtcatt gcgctggcgg acaaaaaatc gcggctgtcc ggcgccgtgg tcatgaagaa
     4741 attcggattg acgcccaagg gcaaggcttt ttccggcatt accgtcgaag acgacaagcc
     4801 cgttcaattg acccgtcaga ttctgaaaag cctgaaatgg atcggccccg cagagtgtga
     4861 atttctgaaa gacgagaaag gccattattt cctgatggaa atcaatagcc gctttccctc
     4921 ctggctttat cttgccgccg ccgccggcca gaatcttccg ctgctgaccg tgcaactcgc
     4981 ctgcgacatg cccgtgcggc cgctgacttc ctacacggca ggcaaattgt ttgtccgcac
     5041 ggtcgccgac gcgctgctgg acgcgcggca gatcatggaa ctgaccgctt ccggggaggt
     5101 gcgcctgtga tcattgccgt gacaggtttg aacacggccg ccgaacccga agccggcttc
     5161 ggcgttctac aatccttgtc cggtggcggc gcaggccatg accggctgat cggattcgcc
     5221 tatcagccgc tggacgccgg attgtttcat tcgggcctga tgcaccgggc gtatgtcgtg
     5281 ccgccgccca aaacggaccg gatggcattt ttgaaaagaa tcacgctcgt taaaaagaaa
     5341 agaggcctgg atatgctgat tcccgccctc gatgatgaga tggagctttt tgtcgatatg
     5401 gcgccgcaat tgcgcgcagc gggcattgcc gtgctgcttc cttccgggaa atcgctacgg
     5461 gagctgcaaa gcgagatgta ccgacagatc ggaaagcagc aggcagccgc cgggaacgac
     5521 gagcaagatg acccgcccgt tctgcctccg gccctggtca agaatcctgt ttccgaacag
     5581 accgcaatgg acgctgccgt gccggactat ttctcggttg ccgtcgtcgc caacagccaa
     5641 agcaggattg tcgcaattgc ctccgtcagg aaactgctga tctcccgcta cggcggcacc
     5701 tggatggcat tgacggtgga caaccatgaa tttctcggcg ttgccgaaga agtggtgacg
     5761 agcacggcct ggcagggggc cttgaccatc gatgtcgcgc gaaatcggca gggagagcaa
     5821 tacctctccg gtttccatcc gacctttccc gactggattg gccttgcgcg gggggccggg
     5881 gcgaacttgc cggccgttct gatcgacctg atcaaaaaga aaagaatcag gggcgtcgcc
     5941 cagaccgcac ccggcaaggt atggattcga tcttccatcg acctggttac cgacttgaat
     6001 cattttgggg atatttccct caacggagaa atgacctatg atcaaaaata agggcacgaa
     6061 gccttatgtc cggccggtca tcaaaaagat ttctttcggc gataccgtga agtttaaaaa
     6121 cgagcatatg aaaacgccag ccgttaaagc gctggagggc ttgtccgtca gcgatctgac
     6181 cgaccgcttc ggatcgcccc tgtatgtcgt gtccgaaggc acgctccgcc ggagttttcg
     6241 ggaagtggcg caggctttta cccggcgcta tccgaggacg atcattgcct actcctacaa
     6301 gacgaattat ctgagcggaa tctgcgccct gtttcattcg gaaggcgcgt gggccgaggt
     6361 ggtttccggg tttgaatatg agatcgcccg gaggctgggc atggacggac cgagcatcct
     6421 ctttaacggc ccgtacaaga agccggagga aatgaaaaga gcctttctgg aaggcgcgtc
     6481 ggttcacctg gattcctttg atgaactgaa aatggccggg gaagtggcgg cttccatcgg
     6541 ccgcaaggtc aacgtcggcg ttcgcgtcaa catgcagctg aattatccgg cgtgggataa
     6601 attcggattc agctacgaac ggggagaggc tttcgaagct tgccggcaga tcaaaaagca
     6661 ccggtatctg caactgtccg gcctgcattg ccacgcggga acctatatca tcgacctcgg
     6721 catctaccgc cgcgtcattg aaaacctgac cgacctgtcg ctcgatattc aaaaagaact
     6781 gggaagagaa gtggagtatc tggacatcgg cggcggatac ccttccggca acagcctgca
     6841 cacgcagctc atgcccggct acaccatcgt tccgccgatg gagcagtacg ccgaaaccgt
     6901 ctgttccgtt ctcaacggaa agctgcgctc ctttacaaag gcccctcggc tgattctgga
     6961 gcccggccgc tccgttgtcg atgaaggcat gcacctgctg acgaaagtgg tttccgtcaa
     7021 ggaaaggcaa aacggcaacc gggcggtcat tgtggacgcg ggcattcatc tcctgccggt
     7081 ttccgcgtat tatcgctatg aaatgtcctc caaccgggac tccaccccaa cgacggaaga
     7141 cgtggatgtc tatggcgccc tatgcatgca aattgacgtc ctcagaaagt cggtgcgcct
     7201 gccggtggcg caggccggcg acatcctcac catccacaaa gtgggcgcct acaattttac
     7261 gcaatccatg cagtttatct acccccgtcc tgcggttgtg ctgctccatg acggccgggc
     7321 cgaaattctt cgtcgccggg aaacctatga agacatcaag gggccggagg caattcccgc
     7381 cagactgctg acctatgctg ctaaacctaa gagataaact tcagggtgtc ttggaggaag
     7441 cggcaggctg gatcaaggct ccggacagcg gaccgaatgc gcttcaagcg gcggtgattg
     7501 ttctgcgctc ttacggccaa gtcgtcttcg ccgcccatgc ggtttccgga ctttttctcc
     7561 tgatcgcgct cgcgaccatt tcccgcagca cggtatggtt ttcgctcgtc ggcattgccg
     7621 ccgcgtcttt gtccgcgtat gccttcaggg ccgaaaggag ttttatccgg cagggcatct
     7681 tcggcttcaa tggcgcgttg ctcggcattt tttggagctg gtactttact gtttccatcg
     7741 tgtccgtcct gctgctggtc gtgatggccg tcattgttta cctggtgcaa tctttcctga
     7801 tgaacaagct gtcgctgggc cggtttaacc tgccggtcat gagcctgccc tctgtgatcg
     7861 tgttgattgc ttcgctgttg atcgtttact ggctggtgta caacgcttgg ctgattccgg
     7921 catccgccat ttacaggatg gggggatacg ctgccgagcc cctgatcccc atggaaggcg
     7981 ttttgaagag tctcctgccc gggctgccgc tgccgcggca cgtcgttgcc tggctgttga
     8041 ttttcaccgg cattttaatt tgctcgcgca tcagcgcatt tgcggccgcg ctgggcgcgc
     8101 tgagcggcta tgccattacc cggatgctgc ccgccacaac cggggtattg tacacatccg
     8161 acctgtttat cggattcaac gccattcccg tgtccatcgg tctgctgggt gttttcctgg
     8221 tcgccaacgg accggctttt ttcttcacct ttgcgggcct tgtcgtctgc tccgttttct
     8281 ggctcctgct gattcagctt ttcggtcttc tgaattttcc tttcctgacg ctgccgttta
     8341 atttgaccat tctggcggtt ctgataacgc tgaaaaaaat gaattcaccg ccggccggac
     8401 tgtatccggt tcccctggag gtgatcacca caccggaaca aatcatcctc tggcataaaa
     8461 ataatatatt gccccgcgct gccgtgaaaa cgccggaaac ggttgcgcag ctgcttttgc
     8521 atccctcccg gttattcagg ccgagccgtg aagaaatcaa tgcggtccag gtccttgtgg
     8581 acggtgcgga gaagattgcc gttctgtccg gtgcaggaac cagcacggaa tccggcattc
     8641 cggattttcg cggtcattat aatttctgga gaaaattcgg cgcggaagat tttacctatc
     8701 aaaactttct gaaccgggaa gacgtccggg cgcgctactg ggctatggag cggcagtttt
     8761 atgccatgat tctgcgcgcc cgggtgaatc ccacccacca ggccgtgaaa tggctggacg
     8821 atcaaaaccg cctttcctgc attgttacgc aaaatgtgga cggacttttc caaaaagccg
     8881 gcgtgaaccc ggaaaaggtc attgaaattc acggcacgga gcatcaagtc cggtgtttga
     8941 attgcggcgc cattggaaac cggcaggata ttgaaacaat gttccccgcc ggaatttaca
     9001 ttccttattg ctacgcctgc tacggttggc tgaagccggc aacggtgctc atgggtgaag
     9061 agctggacaa agtcctgttc gaaagcgctc tgtccaaaat tctttcctcc gatttgctga
     9121 tcattatggg gaccagtctt caggtggatc ccgtggcttc cattccggcc ctggcctgga
     9181 agaagggcat tcccattatt atcatcaatt ccactccgac cccccagaat catctgtcca
     9241 agctgttcat ccattgcccg acggggaaat tcttcaggaa atttcttcgg caggtcgaaa
     9301 agagacgatg atttgaaacc ctcaagcagc aggggggagt ctttataaac gaagcatgct
     9361 ctttatggat atctgaccac ccactgtccg gaagggacca gccgcgaggt tgctcgctgt
     9421 ttggaatgtg ttttctccag gtacggacaa gggtgggata acgccgggcc gcgcgcgagc
     9481 gcgtctgccc ggcgctttga tcaggatgca gcctgcgagg gagttggttt cctttcctgc
     9541 tcccaggctt ttttcatttt catctggtga tcgagcgttt cttctgtgat cttccggatc
     9601 accgctctgg tttcgtcgtc ctggcctgaa aaaaaattat aaaatccttt ttccagaatg
     9661 gcctgttcga aatcatgact gaccgacagc gcgttgatca gggacatttt gcccgatttt
     9721 gctctcgcga gcgcttcctg aatgctggaa atgaacattt tgactgtgta ggttttggtg
     9781 atcttttcat caaacagaac acgatcgtcg cctgccagaa ggtgcaattt cttaattcca
     9841 tccgcgtggt ccgtctcctg ccgggcgacg gtggtcaaca aatcggcata tttggggaag
     9901 cggtcggcaa acagcaggta aagatcgcac atgttcaact cgagctgttc cagcagctcc
     9961 agtatttttt cctgatgttc cttcagcatc ggtgatttgt cctctcccca acgtgacccg
    10021 gcgaatttcc ggagggcatt cgagccggaa tccagaaagc tgccgtggcg agacgcctct
    10081 actcaaaccc ggcaaaacaa aaagatcccg ccggtgcgtc aatattttgg tttaggggct
    10141 ctcaccctcc cccggcccct cccatcaagg gaggggagga tactccgttt tccctcttcc
    10201 cttgtgagtt gccgcttgcc tctcacgtga ggggcctttt tccctctccc cttgtgggag
    10261 agggtcaggg tgagggggtt cctgatgaga ttgcttcagt cgccctgatt actcgatgcc
    10321 ttcccgatcc aggatcagac gcgctgcccg ccagccgttg atctgacagg agcccacgcc
    10381 gtgccatgcc tgcgtccagt tgctggcaat aaacacgttg ggaacagggg ttgtattggg
    10441 aactttttcc cactgatccg gcgtcaaatc aaaaccgtaa accacgccgc ctttgttcat
    10501 cgtgaagtgc tcgatggttc tcggcgtgta accttccttg acgataatat ttttgggatc
    10561 cttgagttcg gggatcactc gggcggccag ggcgatgagc tcgtcgacct tttgcgcctt
    10621 cagcttgtta tactctgccc gatccttggg ccagacagag atgtcggagt aagcgtccag
    10681 cttgacaacg gatttgcctt tcggcgcata tacgggatcg cccaggtttg agtaaatcgt
    10741 gatggccacc gctcccttgt caaagtcgcc cttcatcatg gcctcatagt tcttcatgct
    10801 ttcacggtaa gtgttgtaga agatttccgt atcgttgtag cccagggctt tcagatcgat
    10861 attcagaccg atatacacgc cgaacaggga attggcgggc ttcatcgcgt cgattttcgc
    10921 cagatatttg gccggaagat tttcccgtcc gatcagcttg tcggccagtt gatagggatc
    10981 cgtattggcg acaatgtagc gggctgtgta ggttttgccg tccgccgtcc ggacgccggt
    11041 tgcccggccg ttttccatca gaatttttgt taccagcgag ccggttttga ccgttccgcc
    11101 cagctcacgg atgcggtccg cataaccgtc ggccagcgcc tgggaggtgc ctttgatatg
    11161 ccacgcgccg tcgcttaagt agctttccgt cgctgccata aagatcagcg ccgattcttt
    11221 atctaccgat gcaccgtaga aaacccataa ctgggaaacc accgctttta gatcttcgtt
    11281 tttaaagcaa tgatccatca ggtctttcac ggtgcggtct ttccatttga aaaaagtggg
    11341 ctggctgaac gggaccagaa tctgcgtgaa tgcgccactc acgcttcctt tgcggaagag
    11401 gtcccgcagt tccatcagat ccttaaagag atcggcgcag agaacatgga atttttcaat
    11461 accctcggat tcctccggcc atttcttttt cagttcgctt ttgaaccccg gccagttcgg
    11521 cggaagcgtg atgtccacgc cggggaaaac cgaccggtag aaatgcggga cttcatagag
    11581 ctctactttt ttgtccacgc cggtagcttt gagaagctgg tacaggccgc ggtccatctt
    11641 gccgggaccg ccgccggcca tttcatgaag cgccacgtcg aacacgaact cgccgcgggt
    11701 gaatttggat gtgctgccgc ctactacaaa gtgcttttcc aggaccagca ctttctgtcc
    11761 tttgacggcg aggtgcgcgg cggcggagag tcctcccatg ccggcgccga tgacaatgac
    11821 gtcatagtcg ggttttggcg ccgcgggatt ttcggctgcg taaagaccgg cgttgaagat
    11881 cagaatgatc ggtagaatca tcgacaaaaa aactttgcgg ataaaatttt gcttcttcat
    11941 cggaatcctc catgttacgt gtttgggtcg aattctccca ttaaatgaac ttcttgtcaa
    12001 gattcgaggg ttggaagcag tagggaacgg tcgcgaccat tccctacgag acattgattc
    12061 cggctttcgt cgattccggc tttcgtcgaa gcatgggcat ggcaaataaa attggttttt
    12121 caaatctctc tatgcgcaaa cgggcggacg ccggtccttc cacggcctgg ccttttcaag
    12181 ctggccggca agcctaaaca gcgtggcttc gtcgccgaac cggccgatga aatgggcgcc
    12241 caccggcagt ccttcgctgt tccagaaaag cggaaccgac atggccggct gtccggtcgc
    12301 attgcagatc ggcgtaaaaa cggcgtagcg ggccgattcg gcaaatcccg cgagcggatt
    12361 gtccggcttc gggtcgaacg agcccagcgg cgccggcggc ttggccagtg tcggcgtcag
    12421 cagaatgtca tacttctcaa agaacgaggc gacatcgcgg gcgacgcgct ggatggtcga
    12481 gacggcaatc aggtagtccg cggcgctggc ggtctggctc tgttcgtaga tggcccaggt
    12541 caggggttcg acctgttctt tggatacccc cagcatcttg acgatggagg cgatgccggc
    12601 gaaccaaagg acgacaaagg cgcgggtcag ggttttcccg tcgatgacgg gggcggactc
    12661 ctcaaccgaa tggccgagtt cttcgcacag cctggccgcc tcctcaaccg cgcggacgca
    12721 gtcttcgtgc accggcttgt cggtcggggc tttcgcggta aaagcgatgc gcaggcgccc
    12781 cgggtcggtt tccgtttcct tcaggaagga tcttttgacg ggcggcgccc agtagggatc
    12841 tcccctcaga cagccggctg tcgcgtccag gagcgccgcg ctgtcccgga cggaaatcgt
    12901 caacgcatgt tcattcacca gtccgccgat catgtcgccg aaatcgggcc ccagggtgat
    12961 tctgccccgg gtcggcttca ggccgaaaac gccgcagcag gaggcgggga tccggatcga
    13021 tccgccgccg tcgtttccgt gcgccatggg gacaatgcgc gccgcgacgg ctgcggccga
    13081 gccgccgctt gatccgccgg ttgagtggtt tgtgttccag ggatttcttg ccgggccgaa
    13141 cagttccggt tccgtggtcg ggaggatgcc gaattcgggg gtgttggtct tgccgatcac
    13201 gatcagcccc gcctttttgt ggcgcaggac cagttcgctg tcatgatccg ggaccaggtc
    13261 tttcaagact ttggcgccga gcgtcatgcg cacgccgccg taggaggcca ggatgtcctt
    13321 gagaagaaac ggcacgccgg cgaaagggcc tttgagtcgt tttaatttcg cggcctgccg
    13381 cgccagatcg tacatcggcg tgacaaccgc gttgagggcc ggattcagct tttcgatttt
    13441 ttcgatcgcc gattcgataa gttcaagagg tgtgatttcc tttttccgga tcagaccggc
    13501 cagtgctgtt gcatcacaat atttcagttc ctctgacagg gacatgcgac ctccttttcc
    13561 tggtgatagg tttgcctatg atgcgtcgaa gtatagggtg gccatgcggc ggtctcattg
    13621 aagatcatgg catgcgccgc attccacgga ggttttctcg ccctgatgga ttttatgcgc
    13681 attcgtggaa aaccgcgtcg gttcatcact gcccggtttt gacgcgtggc aggacatgca
    13741 gccggcggtt ttatcgacgg gaatgtgctc tttgggcagg attttggcgt agtcgttgtg
    13801 gcaggcggcg cacgttttgg ctttcagggg cgggccggcg gcggtgacga cggatgcgaa
    13861 aacggcgccc gacaagacgg ttgcgcaaac aacggggatc catgttttcc ggaagttcat
    13921 acgatttaca ctcctttcca aattttcagg tcaaatcccg taaaaacggc ttcaataggc
    13981 gatgaatcgc cgacagaaca acaatgcccg ccaccacgac aaccaggacg gaaaggggtc
    14041 atgcccgtga tggcgacgcc gagggtcgtg ccggccgccg gcggtctgca tccaggaaaa
    14101 gaatacagac gtttgtgatt ttacactccc tggctgaaaa aagaaagccg attctgaata
    14161 tatccggggg ccggtcgttg atgtttaaaa agcagcgata aaagaccggc catgattttc
    14221 ttgacaggcc cgaacccgtt tcctatactt catgcgcgag attcaaactc ggtgtttaaa
    14281 cgatcaacga tatcaggagg atggttttgg gattatcgct tttgatcgtt gccggttcat
    14341 accggcgtct gggtaactgc gaaatgtttg cgaaagacgt cgcgctgcgc gccggagcgg
    14401 attccatctt tctgctgcgc ctgacggatt tcgagttgaa gccctgcacc ggctgccacc
    14461 ggtgcctcaa tccggagcac cgctgccgcg tccaggacga tctgtgcttt attcttgaaa
    14521 aaatgaaagg gttcgacgga atcatctttt ccataccgac ctacgtgctg gggccggtcg
    14581 gccagttcaa actgtttgcc gaccgtctgt cttccatggc cgtctttcat ccggacttcc
    14641 ggaacatccg ggcggtgagc gcgattttcg gcggcattga aagctggcgg ggcgtgacgc
    14701 agtcgctggt gaatgccgtc atccggatga tgggctttga cttccgggga agcgcttttg
    14761 tggaagccgc cctgcccggt gaaagcctgg aggccaaata ccagcccgtg gcgcaggggc
    14821 tggctgacgt attgagcaaa cgtatagaga cgtattttcc ctgcaaaaca ctgcgttgtc
    14881 cttcgtgcgg cgcggatctg ttttttctgg agcaggggcg ccgggtctgc gctctttgcg
    14941 gaagttccgg cgaagagcag gacggcgttt ggttgcatgt tgaagattcg ggacggttta
    15001 cgacgaaagg atttgtcgag cactttgaaa gctggctgaa acccaaggtg ctggaatacg
    15061 ccgcccagcg ggagcatcat cgcgcgctgc gcgaaaccta taaaaatatc ggcacgtgga
    15121 tcaagaagga ggagtagcgt tccgttcgca aaaatcgcga tcattgtcaa ggttggatac
    15181 ctttgaaaaa tgtcatttcg aggagcgcga gcgacgagaa atcttaagga tggccgtttg
    15241 ttcaagattt ctcccttcgg tcgaaatgac aaaaatgcag ttattcaaag ctctcggttg
    15301 atgagttcgt aaaaaacggt caggcccggt gcgccttcaa aatcacgagg gcatcgacgg
    15361 caatcggctt gccgtcgcgg atgatcaggg gattgatgtc gatttcagcg atctcctcgt
    15421 gcttcattcc cagatggcct atacccacca aagcgcgaac cagtatttcg cggtcaacgg
    15481 ctttttcgcc ccggaaggcg tccagaagct ttgccgcgcg gatttcaccg atcatctcgc
    15541 gggcgtcgtc ctccgaaaga ggcgcgacgc ggaagctgac gtctttcatg gcttccgtga
    15601 aaatgccgcc cagcccgaac atgaccgagg ggccgaactg cgggtcgcgg gtcaggccga
    15661 tgacgaattc ccggcggccc tcgaccattt cctgaaccag aaagccttcc agcctgattc
    15721 ccttccctga aagttcgtca aaagcgccgc ccacatcgtc tcggtttttc aaattgacgc
    15781 gcaccattcc catctccgtc ttgtgcgtca gctccggtga cgatcccttg aggacgacgg
    15841 ggaagccgat tttttccgcc tgatccatgg cttcatcgcg ggattgaacc agcgcctctc
    15901 gggggatggg aacgcccgcc gaggcgatca ctttcctgga ttcatattcc gaaagggcga
    15961 gttgtccctt ttgaagggct ttgacgagca gatccctgat ttctttttcc tttttgccgg
    16021 tcattgcatg gcctcccctg ttgatcgatg gttcgctttc atgcccgcgc tgcaggacgt
    16081 cttttcgccg gtaaaattct ttttcaaaag agaggcccaa tacatttggg agacggcaag
    16141 agcccgcacg gcctgttccg ttgtctcgaa gatgggaatg cggctcttct tgcgaatggc
    16201 gatggatttg gaggcggcat tcggcgtgta tatggcggcg ggcttttcat attgcttgca
    16261 cattttcatc agctccagca ggcagtcgat tacaaaaggc tgccagacgc tcagcagcgg
    16321 cagaaggccg tccacttcgt ccgcggaaag aagaatttcg aaaatgtcga ggtacgtctg
    16381 cggaagcgcg gggccgatgt ccacggggtt cttgacattg aaaacctgtc cgactttctt
    16441 cagcttctcc tgcgtttgcg gggccagacg caccatttgc agacccgcct gcaccagcag
    16501 gtccgcggaa atcccgccga aagcgccgga gttggtgaac gccgcaatcc gctttccttt
    16561 cagcaaaggc atttcggtga acatcttggg gagcgaatgg agttcgtcaa tggactgcag
    16621 acggatgatc cccgtctgtc ggcaggcggc atcgaagacc cggtcgttat tggccattcc
    16681 cgccgtgtgc gacatggcgg ccatggcgcc ttccttggtt ttgcccacct tgtaaagcag
    16741 gacgggcttg tgggatttcc gggccgcctc catgagcctg cggccgttgg agatgttttc
    16801 cagatacagg ccgatgactt cggtttcgtc tttttgaaaa tattcgatca gatccgcttc
    16861 atccacatca cacttgttgc cgatgctgac cactttgttc acgccgacga tgtcgtaata
    16921 aagcgattcc atgaccagga cggctacgcc gccgctctgg atgatgtaag agatgttgcc
    16981 gggcgactca aaaatctgat tcatttcgac caaactcggg ttgacgccgt aaaaacagca
    17041 gaatttatcg cgggtgctca gggcgccgag gcagttgggc cccaggatac ggatgccgtt
    17101 ttgtcttgca atcgtgtcga tttcacgctg catggcttcg ccgatttcac cgccttcgga
    17161 aaaaccggcg ctttcaatca ctacgcgcct gatgcctttg cgggcgatgt cctcgataag
    17221 gccgggaaca tggtgcgccg ccacgatgat gatcgccaga tcgggggcct ccggcaagtc
    17281 cagcaccgac gaataacccg gacagccgtt caccgcctcg cctttggaat ttacggcata
    17341 gacggctcct tgataatgga gataatcctt cagcatgttg cagatggtgt ggcccaggtt
    17401 gaagggggta ttggacgcac cgataacgac gacggaccga ggatcgaaga agagttccat
    17461 aagcgcctgc ctgagatcga attttcgcgc cggtataaaa cgcaaaactt aggccttttg
    17521 tcaagcagga aaaaagccgc tcgatttttc tgaacatcgg atcagatcag aagggcgtta
    17581 cagggttccg tccagcgcgt cggccacttc ggccttgcag gcccggcagc gcttggcccc
    17641 ccggaagagg ggtttgccgc aggccgggca gtggtagcgc tccagttccg cccgcgccca
    17701 ttcctcggag ccctgctcgt cgcccagcgc cgcagtcttt tcgcgccaca ggggaatcgc
    17761 ccttttcatg acgcgaagtc ccgtcgccag tccgaaattc tcgatcaggc cgcacggcca
    17821 ctgatcgcac tggtggcagg aataaaatcc ctttttgcgg acgcaggttc ggatggagca
    17881 attcatgcaa tagccgtaga gcttttcggg cggatcgctt tgcatgcagc ccaggcattg
    17941 tgtgtcttct ttttttgtgc cgtagaggtt gcccatgacc tctctgaatt tttcgttatg
    18001 gtcccgcgtg gcgatgtaaa cgccgcaggc gccgcagtaa aggccgcagg gggccatcag
    18061 ccgccggtct cttatttctt cttccgtcca gccttccatt gccgcaccgc cttctgttca
    18121 gagcatagcc ttcccccgcc ggttcgccgg gggaaggccg tcgcgtgtaa aggtttttaa
    18181 aagccgagcg acaggccgat gtaaggccct gtgtagaggg tgtccatttt gtaatcgttg
    18241 acgtccattt tttgctgaag cagccggtag ccggcatgga tgtcgaggaa cggaaagggc
    18301 gtgagggaaa ggtccgccat ggcttcatag gaataattgt cggagccgta tccgccgccc
    18361 gttgcctgtg cgcgaacttc cagcaggttg gccaggatgc cgatgtgggc gcccaggccg
    18421 atcatcggaa tcacggaatt gaaggaattg ctctgttcca cctttccggc cgaattcagc
    18481 ttgaaacttc ccgtggaata cttgatctgg gcgatgccgc ccagggagaa gccggccaga
    18541 atgttttcca tgttgatcag gtcgcgctgg taccgcagat cgaacatgga ataggacagg
    18601 tccgagtgga cggcgcttcc ggccgtgtag ttgatgccgt tgtaattgag ggccgccgcc
    18661 aggacgttgt ccgttgtgta gccgaagggc gtgaacatca ggctgatatg gtttttcccc
    18721 acgcccccgt aagcttcgac ggagaaggtt gccgtgtttc cgacgcccag catgtctctc
    18781 gcgtcgatgt ccgttccttc gatgccgtcg gcgatcgtct ggatatccga ctttttgaga
    18841 tcggggaacc agacgtaccc tcttgcccct atttcaaagg cattggcctc ggcgacgatc
    18901 agcatcgcaa aaataagcgt cagggctaaa acaattttcc ttccatggct tttcataaga
    18961 ccctccattt tgatgaatga tgattcgcaa tctcgcatat ttccaaacat tcgtcaatcg
    19021 gcagcatcag caaaatttat tcaatatccc ggcgcggccg gaggtcactt cccgatgacg
    19081 ccccggaacc gggcgtattc caccatgcgg ctgaaggcaa agatggcccg ctccggcgtg
    19141 gggtagcagg gaacccccag cgcggcaaag ccctcatgga tgtgggcgat ttcccgcgcc
    19201 gtgccgccgt aatagctggc aaagaccggt ttgtgcggat ggcgccggaa aatgtcggcg
    19261 aaggcttcgg gaatgtcaaa catgctttcc gacatgagca cgaaggtgat cagcagggcg
    19321 tccacatcgg gctgctccat caggcagcgg gcaatgtggc tggtggcctt cttcgatccg
    19381 tgctgttcaa tggcggacca gatgtcgatg gggttgcgca ccggcgccca caccggcatg
    19441 acttcgcgaa gcttcctgag cgtgtccggc tgcaacgcgg gcagatcgat gccgcattcc
    19501 tcgacggcgt ccgtcgcggc gacgcagccc atgcccgtga tggacagagc gcccatacgg
    19561 tttccgcgag gcagagggag cttttcaaac gccgcgaggg cgtcaaagaa ggcctcggag
    19621 ttttgcgtcc gcaccaggcc ggttctgcgg cagaccgcgt caaagaccgc gtccgatccg
    19681 gcgagcgacc cggtgtgcga cgccacggcg gccgccccgg cttcgctgcg ccctgctttc
    19741 atcaccacca cgggttttgc tttgcaggcc gcggccgcgg cgcggatgaa tcgttttccc
    19801 tccaccacgc cttccagata catgccgacg gtgcgggttg ccgaatcccc ggtcaggtac
    19861 tccagcagat cgctttcgtt gacgtccccc ttgttgccga tgcaggccac cttggccaga
    19921 ccaaagggct tggtgtccgc aatccagcgg gcccatccgg acgagaagac gccggactgg
    19981 ccgatgatgg aaacgccgcc cttcctgatc ggatcgagcc cgacaataga ggtggacatg
    20041 ccggcggacg ggttgagggt gccgatgctg ttgggtccca tgatccggat gccggccctg
    20101 cgggccattt cgatgatggt tttttgttct ctggctcctt cctcgcccgc gtccgagtag
    20161 ccgcccgtgc tcagaatgat gttggtgacg cctttggcgg cgcagtcctc cagggcgtcg
    20221 ggcacggccg ttttgggaat cacgaccatc gcggtttcca ccttccccgg cacgtcgctc
    20281 acccgggcat aggaggggag ccccaggatt tctttgacgg tcgggttgat gggataaagg
    20341 gcgcctttga aaccggcctt ttgcagattc cgcaaaatga tgttgccgcc tttgcccgga
    20401 ctgcgagagg cgccgatcac ggccatcgat gaaggctgga aaaacggcgc gatggtggcc
    20461 gggctgggtc gcttgcgggc tggggcggga gaagcgccgt ccagccgcac cagagcgtca
    20521 acggccacgg cgccctgcgg ataggcgatg aaaggattga tgtcgatttc ggcaatgtcc
    20581 ggacgatcgg cgatcagacg ggatagcgcg gacaatgatt ttgcgatggc ggcaacgtcg
    20641 aggggctccc gtgaccggta gccgtccagc agggcgacgc ctttgagccg ccggagcatg
    20701 gccggcgcgt cttcctcgtc caccggggca agctcgatga ccgaatcgcg gaaaatctcc
    20761 gtaaaaatgc cgccgatgcc cgccaggaca atcggcccga agacgggatc gcgcctgcac
    20821 cccagaatca gctccgtgcc cggacgggcc atcttctgca ccagcagcgc aaagggaatg
    20881 tcgggaaatc tttttttcat ttcgcggacg gcccgctcca gtgcggccgt gtccttaatg
    20941 tttaaagaga cgcctcccct ttcggtcttg tgcggaacgg cctcggaaca gattttcaac
    21001 gcgaccggaa atccaagccg tccggctgct tccgctgcct ccgcatcggt atgggccaga
    21061 atgccgtcga tgacgggaat tcccgattgt ctgagcagag acgctccttc aaattcgtta
    21121 aggattttta aattcttctt caaaatgcaa agacctcctt gcagggcgaa cattgccaaa
    21181 attcacgccg agaccctatc atgatttttt aaaataattc aacttgcata ttgcggccga
    21241 tccgtccgtc gatcttttga tcgattccag ggaggaaaac gccagaggcg gccggacggc
    21301 tgccagggcg ctgaccgtga cgggaacgat ccggcgcggt ctggatgagc cggagaaaaa
    21361 cacggtccgg gcggtcctgt gccggcgcca tcctgatctg aaggattttt tctccgatcc
    21421 gtccgtggaa gtgatcgtgg tgaaaatcaa cacccttcag ctgctcaacg gcataacaga
    21481 ctcgtatttt gaagaagttt gagcccaaac cccgaatgca gggccggccg ccccctgacg
    21541 ttggggtttt tattgacaag aaaaccgttt tcctctatag ccccttctac aaattgtcct
    21601 gaaaatgtca tacccaaaga aacatccccg gtatggccga ggagtgcccc ttctgtactt
    21661 tggttctttc atcgaagcaa gctttgggtc atctgacccg aagatattaa aatccctgga
    21721 ggaaatatat gtatcaattg gtcaaaccgg ataatatcgt ggaaatcctt gagaaaactg
    21781 tcgccaaatt tcccgagcgg ccttttctgg gaaccaaaaa caaggcgctc aaacagtatg
    21841 actggatatc ctacgccgat ttcggcaaac gtgtggatca cgcaagaagc ggcttgagtc
    21901 agctgggaat tcaaaaggac gatgccgtcg ggatcatttg caataacagc acggactggg
    21961 cggtatgctt ttttgccgta agcggtttgg acgcgcggtt tgtccccatg tacgaagcgg
    22021 agctggcttc ggtgtggaaa tacattattc aggatgcgaa cgtcaaattg cttttcatca
    22081 gcaagaaggc catttatgag aaaatcaaag acgccctggc tgccatgcct tccctccaga
    22141 aaattattat catggagggt gaagggcccg atacgctggc ggagctggag aagaaaggcg
    22201 caggcctgaa agtgaaaagc atctacccgg ccccgtctgc gatctgtatt ctgatttata
    22261 catcaggaac gacgggcgat ccgaagggcg tcctgctttc tcaggccaac attacgagca
    22321 atgctcatgc cgtaacggtt tgcttcccgg atatcaatga aaacgacaac tccctgtcca
    22381 tactgccctg ggctcatgtc ttcggactcg gagagcttat tgtatactgc ctgctgggcg
    22441 cctcgatggg tcttgcggaa agcgcagcaa ccatccccga ggatttggct cttgtgaagc
    22501 cgaccttcat gacggccgtt cccagagtct ttaacaaggt gtatgacgga ttatggacga
    22561 agatgaatga ggaaggcgga ttggccaaga cgttattcgt catgggcgtg gagagcggca
    22621 agaaaaaacg ggagctggct gcacagggta aatcaagctt catgacgaac ctgaaattta
    22681 aaattgccga tgcagtggtg ttccagaagg tcagggaaaa actcggtggc agattaaaag
    22741 gtgtcatgac cggaagcgcc atgatgaatc cggaaatttc tcattttttc tgggatatgg
    22801 gtgttccgct ctatgatgct tacggattga ccgagacctc gcccggggca accatgaact
    22861 gtcccgccaa atacaaaatc ggcagcgtgg gcccccccat ggggctgggc aaaatcgtga
    22921 tcgacaagtc ggttgtcggg cccgacgcaa cggatggaga aatcattatt tacggaccca
    22981 acgtgatgca ggggtaccat aacaaacccg aagccacaaa agccgtcatg acgccggacg
    23041 gaggattccg gaccggagac cgcggccgtc tggatgagga aggcttcctg tttatcacgg
    23101 gacggctgaa agagcagttt aagcttgaaa acggcaaata tgtatttccg gcggccatcg
    23161 aagaagatat gaagctgaat cactatattg aaaacgccat gatctatgga gaaggccgcg
    23221 agtttaatat cgccatcgtc attccggatt ttgccgccct cgataagtgg gccgcgaaaa
    23281 acaacctgcc caaggatcat gccgagcttt tgaagaggga tgaaaccaaa aaattcctgg
    23341 aaagtgaaat attgaacgcg ctgaaatcca cctacggcag ctatgaaatt cccaggaaat
    23401 tcgtctttat cgaagagccc ttttctctgg aaaacggcat gctgacgcag accatgaagc
    23461 tcaagcggcg cgtcgtgttt gacaaataca aagaccagat tgaaaagctg tatgcaaaat
    23521 aaacggttcc cggcgctttt tttcagcccc ggcctttcct gatgatacgg ccgaggcgcg
    23581 gcggatgacg ttcaacagga aacctcacgg tcaaccaggc cgtgaggttt cctgtttttc
    23641 atggaaagcg gaccggagaa atttcgacca gcgctacggc catccggtca atcctggaca
    23701 ggggcagttt tttcaaatcc gcggcggcag cccatcgcca gtcctggcag ccccgggcag
    23761 tcggtttgcc gcttaagggg aatccctgat aaacatgaag cgtcacgcgg aaatgcgtgt
    23821 aggcgtgatc cgcggaagcg atctttttcc cgacgcggat ggaaaggccc agttcttcct
    23881 ttacgcagcg tcgcaaaccg gcttcccggt tttcatcatc ttgcacaaaa ccgcccggca
    23941 gtttccacaa cgatgccagc aggccctccg cctgccgctg cacaaccaaa agaaggcctt
    24001 tgccgttgcg gacaacggcg gcgacggcct gccgatgagg gatcgcgggg gacttgcgcg
    24061 aaacgggcag atcgttttgc aaaccgccgc gccgtgccag acagatcgac gccaggggac
    24121 agacgccgca gtccggattc ttcgcccggc agattgtcgc gcccaggtcc atcagcgcct
    24181 ggttgaagtc ggaggctcgt ttgcccggaa caagcgaagc ggccagttcg aaaaaatgct
    24241 tttgcccccg cggatcgttg accgggcggt cgatagcgaa aacgcggctc aaaatccggc
    24301 ggacgttgcc gtcgacggcc gggacggccc ggccgtaagc gatgctcaaa atcgctcctg
    24361 ccgtgtagtc gccgatgccg ggaagcgtcc ggatgtcttc tggcgtgtcg ggaatccggc
    24421 cgccgaacct gccgacgatg atcccggccg cggcgtgcag atggcgtgcg cgcgaatagt
    24481 agccgagatt ttcccaggtt ttgagcacgt cctgcagcgg ggcgcgggcc agcgcgaaga
    24541 cgtcgggaaa ccttttcaga aaacgtttgt agtagggaat gaccgtgtcc acccgggttt
    24601 gctgaagcat gatttcggaa atccagatgc ggtagggatc ggttgtctcc cgccagggaa
    24661 gcagccgccc gtggcgttta taccaggcga gcagccggct gcggaaaaaa gtcttgattt
    24721 cagcttggcc tttcgacatg gttcccggcg ctatcgaatc agtttggcaa tccggctcca
    24781 gcggacggtt ttgtcttccg cattccacga ccagtaaaga ataaaggcct ttccctgcac
    24841 gtcttttaaa tcgacaaatc cccagaaacg gctgtccaga ctgtggtcgc ggttgtcgcc
    24901 catgacgaaa agcgattttt ccggaaccac taccgggccg aaattgtcgc gcggctggac
    24961 gtcagaggga agcatcgtgt gatcgtcata gacgccgtaa ggatccgcgt aaggctggtt
    25021 gttgatgaag agcttcttgt ttttcatttc gatcgtgtcc cccgcgacgc cgatgacgcg
    25081 cttgatgaaa tccttggagc ggtcttccgg gtaaatgaac acaacgacgt cttcccgttt
    25141 cggatccgtg accgggatga tggtggcgcg aaaatacggg agcttgacgc cgtaaatgaa
    25201 tttgttcacg aacaaatgat cgcccaccaa cagcgtcgga atcatggagc cggacggaat
    25261 tttaaacgcc tggacaatga acgaaatgat aaaagcggcg atgagcagcg ccgtcagaat
    25321 cgcctcgata aattcccgga tttttgattt ggctggagcc ggttttgatt tgttttcttt
    25381 catgattctc cctgaaatgc cttgcgggta ttttatgcaa gactttgata ttacttactt
    25441 ttctattcga taattaaaag aggcgataat atgcaggcgc gacaggtcgt attgccgggg
    25501 cagcggctgg taaggctccg ccgcccggac aacatccagc gtgcttgaat caagatggat
    25561 aaaaccggaa gaattcatca gggccacctg tgaaagcctg ccgtttgcgt caatcgacat
    25621 tttaaccacc accactcctt cttcttccat ttgatacgcg gcttcgggat atttccaaat
    25681 ccgcaaaatc ttttgcttta cgcccgacag ataggctgca tatttgacgt cggaagaacc
    25741 gatgttcacc gtctcttccc tgttgcccgc cggggccggt ttcgcgtccc ggctgtcctc
    25801 cgatactttt ttatctgtgg catccccgtc cttttccggg gtgacgggcg gtggaggacc
    25861 ggcaatgtga acggtgaaag gctgtgccgt cttttcctgg ttgcgcaaat ccaccatgct
    25921 gctggccgta atcagcgcgg catgtccgat cagggatatc atcaccggca aaaggaaaag
    25981 gttttttgag tccatgttct cccgttcgcg aagcgatcat atatggcatt tgcctcaaaa
    26041 tcaacaatat gataaccgaa aaaacgattt tagattcggc agggctgatg ctgacggacg
    26101 aagactacat gagaatggcg ctggacgagg cccggaaggc tctcgggcgc aacgaagtgc
    26161 cggtgggcgc ggtgctggtg caggatgacc gtcttctggt catggcgcac aacgcgccca
    26221 tttccggggt tgacgccacc gcgcacgcgg agatgaccgt catccgcgag gcctgtcgga
    26281 tgtcgggcaa ctaccgcctg acgggcgcgc ggctttatgt gacgctggag ccctgcctga
    26341 tgtgcgcggg cgccatcctg caggcgcggc tggcgcgggt ggtgttcggc gcgcgcgatc
    26401 ccaaggccgg cgcggtgttc tcgctgttcc aggtgttgca ggacagccgc ctgaatcacc
    26461 gggtggaggt gacggaggga atcctccggg aagaatgcgg agaaattttg agtagatttt
    26521 ttaaggaaaa gagggtagaa gccggctcgg tgagtccatg aggcgcgaaa aaagggattt
    26581 ataataaaat aaaagcgcgg agagatgtct gagaggccga aggagcacga ctggaaatcg
    26641 tgtgtacgcc cacaaaggtg taccgcgggt tcgaatcccg ctctctccgc catttttaaa
    26701 tcgctgtatt tgacgacttc gtagaaaatc ctttttgcat aagcacaaga ggcatcatat
    26761 cgcacggaga gttaccgaag tggtcgtaac gggatcgact cgaaatcgat ttaggggcca
    26821 caagctccta cgggggttcg aatcccccac tctccgccat tcatcttaaa atttggttga
    26881 ttcctgtcga tttatccatt aagagtctgg ccagaagaat
//
LOCUS       DAOSRM010000020        24654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2205_length_24654_cov_111.238668, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000020 DAOSRM010000000
VERSION     DAOSRM010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 24654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24654
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2205_length_24654_cov_111.238668"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..633
                     /locus_tag="PLA58_03515"
     CDS             154..633
                     /locus_tag="PLA58_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61894.1"
                     /translation="MDGPVGGIAYATPSLKGVTKADGVFEYRPGETVAFSVGELALGS
                     AAGKPVVTVLDLVPDAKDASDQRVVNICVLLQTLDQDGDPANGILISEQAASFVTKYG
                     KGTNFNQHIRSFSFDSGFRSLMAELNNVDAFGETPRAVVPGKIAQKHLEATLAGLKK"
     gene            complement(745..1701)
                     /locus_tag="PLA58_03520"
     CDS             complement(745..1701)
                     /locus_tag="PLA58_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR61895.1"
                     /translation="MKTKDIYLQDIQPGDKIASSFLVAEKSMAFSQKGSAYLNVRLKD
                     KTGETDGKVWDNAVALDRVFKKGDIIFIEGRAQSYRNALQISILTAKPCDPQDIDPSD
                     YLPVSKLNTAAMFENLLAYVDTISSDPLKKLLGAFLNDEKTADLFRRAPAAKGFHHIY
                     LGGLLEHTLSVVRLLDQAAKRYPQLNRDLLIAGGILHDIGKIYEFSYDGLIEYSDEGR
                     MIGHLVMGVEMINKKMEAIGNFPERLALELRHIILSHHGEFEFGSPKRPKTREALVIH
                     FMDDLDAKLNAFDSFVEADAANAGSEWTAYNRFFERYLYKGK"
     gene            complement(1730..3643)
                     /gene="selB"
                     /locus_tag="PLA58_03525"
     CDS             complement(1730..3643)
                     /gene="selB"
                     /locus_tag="PLA58_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOR61896.1"
                     /translation="MQHFVLGTAGHVDHGKTSLIKALTGVDTDRLKEEKERGITIELG
                     FASLLLPSGHTLGIVDVPGHEKFIRNMVAGAAGIDLVLMVIAADEGVMPQTKEHLQIC
                     SLLGITTGLVALTKIDLVEKDWLDLVRSEIADYLQSTFLAGAPIVPVSASQTQGIPEL
                     LGEIDKTVRHLQEKTDDGIFRLPVDRIFTMKGFGTVVTGTLISDKICVGEEVQILPEN
                     IFARIRGIQVHNQPTEEAFSGQRTALNLQGIEKSTLERGNVLVRPQTVRPTQRLDVFF
                     EYLAGNARKLKNRSLARLHTGTAEIMTRIVLLDADELSPGDQSFAQLILANEDVVVGG
                     DHFVLRSYSPITTIGGGRILDPQPGKHKRKNKKILDDLKILQSGGLQDKISVLLDRAG
                     FNGLNVPSLAFRLGISAKKIREALEKLFSDRRAILLPGDDATALSADLCAHLEERLMK
                     SLADYHANHPLKEGISKEELKAALPDAVSGKLFNMVLARLIKKEGIASDKDHVRLATH
                     QVQLAGEEDALRQSLASVYLQAGLAPPSLSDVMTRFKDQKVKAQNIVRLMIKDGELIK
                     INEELCFARTALDKLREDYKAQLVRDGQATPATFKDLTGLSRKYIIPLMEYFDASKLT
                     VRVEDHRILREKP"
     gene            3807..4232
                     /gene="ruvX"
                     /locus_tag="PLA58_03530"
     CDS             3807..4232
                     /gene="ruvX"
                     /locus_tag="PLA58_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOR61897.1"
                     /translation="MRILGVDYGEKRIGLAVSDELGLTAQGLPTLIRKTRKNDLEILR
                     QWIRNYSVQKIVIGYPLRIDGTEGIQCEKVNRFARWLAESFLLPVMKWPETLTTKEAE
                     DILRASGVRWQKRKEKVDQLAACLILQNYLDTAGRGEQG"
     gene            4289..5332
                     /gene="mltG"
                     /locus_tag="PLA58_03535"
     CDS             4289..5332
                     /gene="mltG"
                     /locus_tag="PLA58_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOR61898.1"
                     /translation="MKSKKSKIMLYVLSLPVIVALVFGAILFSYAVTPIHSAKEKTVV
                     VDIPTGSSLIQIIKILHDADLVQNRFLFHVLVTVKGGARSVRAGEYEFAATITPSHLA
                     DKLLHGDIKKYPVTIYEDSSLSQVALRLQENKLINEKDFFELSQDAEFLSELGIRSSS
                     MEGYLFPDTYFLDRSMTTRRIMRMMVERFQSKVSPEMIRQAAQKGLNVHQFVTFASLV
                     GKESGCAAEKPLIAAVFYNRLKKGMPLQSDPTAVYDLKNFKGKVMRSHYKRESPYNTY
                     LIRGLPPGPIASPGLDSFQAVLQPAAVDYLYFVSKGDGTHHFSSSLMAHNDATVRFRN
                     MINRKEKVEETVR"
     gene            5611..6057
                     /gene="mraZ"
                     /locus_tag="PLA58_03540"
     CDS             5611..6057
                     /gene="mraZ"
                     /locus_tag="PLA58_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOR61899.1"
                     /translation="MSVFRGHYHHTIDEKGRIIFPSRFRDTFAEHYDNKMVITNWDGY
                     LIVFPYAQWLLIEEEVLRRRILDKKIRDFQRFFMSGAVDCALDGQGRVLIPPALREYA
                     KLEKDIVLVGMLKNIEIWPREKFEERNKEAGEKIDRDNEIAESLGI"
     gene            6054..6995
                     /gene="rsmH"
                     /locus_tag="PLA58_03545"
     CDS             6054..6995
                     /gene="rsmH"
                     /locus_tag="PLA58_03545"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOR61900.1"
                     /translation="MTVTAGFAHEPVMRDEVLSWLVGNKTGVYVDGTIGGGGHALAIL
                     ENTRAHLIGIDRDAEAIQAARQRLEPFQSRVTLVKGNFADLASVLGGLGIEKVDGVIL
                     DLGVSSHQLDAAHRGFSFGRSAPLDMRMDRDEKLRAYDMVNGFTPSELEKVIRLYGEE
                     KMAARIARAIVRRRQASPIETTAELAALIAQAMPQSMKHQKIHPATRTFQAIRIAVNR
                     ELDALASGIHAAVAALSAGGRLGVISFHSLEDRIVKHTFLDLAAACVCPKEVPYCICH
                     KQAVVKVLTRKALTPSPEEIRRNPRARSAKLRVAERI"
     gene            6997..7344
                     /gene="ftsL"
                     /locus_tag="PLA58_03550"
     CDS             6997..7344
                     /gene="ftsL"
                     /locus_tag="PLA58_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOR61901.1"
                     /translation="MAQTAGTQAITQGRPLKEADAASFGVKYPTFIVVALVIMFVAVI
                     YVGSHIRMTQMEYDVAAALSAKENLLEEQKRLKLELAMLKSPQRIEDIARTELQMTYP
                     TAAQVIVLKQTRK"
     gene            7347..9335
                     /locus_tag="PLA58_03555"
     CDS             7347..9335
                     /locus_tag="PLA58_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOR61902.1"
                     /translation="MTADSKKWLKFRLASILAIFLILFIALLSRAFQLQVLSGEKLKK
                     LAVRQHITTLPIHSERGMIYDRNGEKLAMSVLADSVCADPTRISDPARVSREIASILN
                     MDPSVVLKKISEPKSFCWLARKISSQQALMVESANLEGIFLIKEPKRFYPNGQLAAHL
                     IGFSGFDAEGLEGLEKKYDPYLRGTPEKLTWARDAKGKKLFVHVEHDPSAKSGSANLV
                     LTIDSRIQYLAETHLKDAVLEKGAKGGVAIVMDPKTGEILAMANEKGFNPNNIKGLTS
                     EAWRNRAITDSFDPGSTFKPFLVAAALEEKVVKESDRFYCENGHYQVANRVIREANHK
                     KHGYLAVRDIIKYSSNIGSAKIAEKLGREKFYAYIKNFGFGSRTGIDLSGEAAGVLQP
                     VRSWTRVDTTNIGFGQGISVTAIQLITALSSIANGGVLMKPYIVKGLTDKNNQPVKMF
                     APEAVRRVVSPDTARRMAVMLTDVVGAEDGTGKRAHIMNVAVAGKTGTAQKYDFKRGA
                     YSSEKVRTSFIGFFPSDNPQVVILVMLDEPQRDKWGGVAAAPVFKNIGEQILNCFKTN
                     IREAPAFDPQPVHDLQLVSADASLMEDLAVESNEIDDESRMPDFKGLTLREALKMARS
                     RSIELRVSGNGWATKQVPAPGSDLGEDRLCKVFFEMKN"
     gene            9339..12305
                     /locus_tag="PLA58_03560"
     CDS             9339..12305
                     /locus_tag="PLA58_03560"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOR61903.1"
                     /translation="MMKLRQLLQGVRTVRTETDPQGDVSSICYVAEACGPGSLFVAIP
                     GLAFDGHHFIGRAIERGARFIVHQKDIEVPDGVTAVQVSDSRRALGTLAKNYFGDPSS
                     RLTLIGITGTSGKTTVSYLLESILTAAGFRCGVLGTINYRYNGKFFPAPNTTPESYDL
                     QRILNEMADAGVTHVIAEVSSHALDLKRVDDCDFDLAVFTNLSPEHLDYHKDMEDYFR
                     AKQRLFAELLPRSRKNLPCKAVINADDPRGRRLIGEISVPVLSYGLENAGGVSAQNAV
                     VSLRGIRADIRLNGEKIPVSSGLIGGFNLSNILAACAAASVLGAEPRAIAAGIQNLSF
                     VPGRLERVVPSGGVHVFVDYAHKPDALEQVLRNLHGLKQKRILTVFGCGGNRDRAKRP
                     MMGGIATEWSDLTIVTSDNPRREDPLEIIREIEAGIDETRIRKASPEGLEFDAAGHVY
                     TVLADRRAAIEAAVRIAQPQDIVLIAGKGHEDYQILGTKKVPFDDRLAAARALRLKFS
                     GRMSPAFSLAEVLAATGGRLAAGDADQTVYGISTDSRQIRPGNLFVALAGEHFDGHAF
                     VAKAVEEGADGVLVADAGKIKIGTANQQIGVIEAADTLRALGDLAHAHRRRFSIPVIA
                     LTGSSGKTTTREMLACILGQEKNVLRTEGNFNNLIGLPQTLFRMTDQHDAAVLEMGTN
                     TRGEIKRLTQIAAPDIGLITNVGPAHLAGFGSVEAVREEKGDLWLHMDPAGTAVVNLD
                     DEAVRRTADRWSGRRVTFSMSAGADVGVNDIRRNGARGVSFHLLMNGQAHKVDMKVAG
                     LHNIYNAMAAAAAALACGAGAESIRRGLSMFQPVGGRMEILRLQNGAYLINDAYNANP
                     ASVREALLTLRDLMNSHDAFVLLGDMLELGDAAPEMHRKIGLLLATIGVAAVFLQGDF
                     AQATAEGAREGGLAAERILFLQDDEEAVAFLKRKLRKGDWILVKGSRRMKMDRLAARL
                     GEAIGVEKAEDQEAAL"
     gene            12318..13397
                     /gene="mraY"
                     /locus_tag="PLA58_03565"
     CDS             12318..13397
                     /gene="mraY"
                     /locus_tag="PLA58_03565"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOR61904.1"
                     /translation="MIYEFLYPLHTVFSSFNVFRYITFRTIFASITALLICLALGRWF
                     IEKLQKMQIDQQIREDGPQTHLVKKGTPTMGGMLILFAVTVSTILWANLSVVYIWLVL
                     LVTVGFGLIGFLDDYRKLAVRNSRGVPGKVRLAAEIIIALFVGVVLYFKPGFSAQVTI
                     PFFKTVLPNLGWGYVLLCVFIIVGAANAVNLTDGLDGLATGPAGICFVTYLLFAYFAG
                     NVKVAHYLQIPYVPGAGELSVFCGAFMGAALGFLWFNAHPAEIFMGDVGSLSMGGALG
                     TVAVITKQEILLAIVGGVFVMETFSVIFQVGYFKLTGGKRIFRMAPIHHHFELKGWAE
                     PKVIVRFWIISILLALLAISTLKLR"
     gene            13405..14748
                     /gene="murD"
                     /locus_tag="PLA58_03570"
     CDS             13405..14748
                     /gene="murD"
                     /locus_tag="PLA58_03570"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOR61905.1"
                     /translation="MQLADRKIAVIGMGKTGIAAAAFLGGSGAAVVAVDEKPRDQWGG
                     EFEQIAHRPWLAVGRYDETCLDGVDLVVPSPGIPPRHPLLTAAQQKKIPILSEVELAC
                     RFIRVPIIAVTGTNGKTTTTTLLGEILARAGKKVFVGGNIGNPLIEYAGAAQTDDFIV
                     AEISSFQLQWIESFRPHAAILLNITCDHVNYHGSFEEYRRIKGRIFENQRENDLAVLN
                     AEDPQQEGLDASMRACVVRFSSVRPLAPGLCLQKDSLVYSGPDGTDERYPLGMIRIPG
                     LHNVENVMAAVAVARFCGCSKESIVEAVSQFRGLPHRIEFAGEKRAVQFYDDSKGTNV
                     DAVVRALQTFSSPVILLLGGRDKDGNFDVLQPMLGAKTKQVVLFGEARDQIEPQLGNT
                     TPIAKEATLKAAVLRAYRDAKPGDIVLLSPGCASFDAFANYRERGNHFKEVVRNL"
     gene            14749..15855
                     /gene="ftsW"
                     /locus_tag="PLA58_03575"
     CDS             14749..15855
                     /gene="ftsW"
                     /locus_tag="PLA58_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOR61906.1"
                     /translation="MDAAKKDANTPDVILLLVTLILVTVGTAMIYSSSSILAMEKFKD
                     GQFFLKKHLFFVFAGLVIMIVMTKIPYEQLKKLAYPGVIVSVVLLLLLFIPHVGVKRG
                     GATRWLNLGVFSFQVTELVKVAMVLFLAHLLTRKARHLKKFSRGVLVPLSVTAVVMGL
                     IILQPDFGTVVIIMSILLLMLSLAGSRITHLVFLGAAFIPVGIWLIMSKGYRMARLTA
                     FLDPWKDADNTGFQLIQSLISFGSGGVTGQGIGDGMQKLFYLPEPHTDFILAVIAEES
                     GFIGVTIIIFMFIFFLLRGFMIAFRAPNLFGTLLAAGLTMLITLQAFINIAGVMGLIP
                     LKGLALPFLSYGGTSFVMSMMTVGILLNISTQRT"
     gene            15908..17005
                     /gene="murG"
                     /locus_tag="PLA58_03580"
     CDS             15908..17005
                     /gene="murG"
                     /locus_tag="PLA58_03580"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOR61907.1"
                     /translation="MRIIIAGGGTGGHLFPGIAVAEEFQRRDAQNRVIFIGTSRGIEY
                     RLLEKMGYELKLIDVEGLKGRGWKALIKSVYAIPVSMMQSRRILADFRPDIVLGVGGY
                     ASGPAVMAAYLTGCPTAIAEQNALAGNTNRILGKFVDRIFLTYERSKGRFPARKVRVT
                     GNPVRAAFAARLKEAPKTRAFRQILVFGGSQGAAAINKTVVAMLPMLQKIKDRVRVVH
                     QTGDRDLAMVRQAYEQAGVEADVQPFIVDMASAYAASDLIICRAGATSLAEITAAGKA
                     SVLIPFPYAADDHQTENARAMVEAGAAAMIRESDLTAGALYSLVASLLGDDNKLRGME
                     EKSKALGRPDAAARMVDECIKLAAENQHSTG"
     gene            17013..18425
                     /gene="murC"
                     /locus_tag="PLA58_03585"
     CDS             17013..18425
                     /gene="murC"
                     /locus_tag="PLA58_03585"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOR61908.1"
                     /translation="MSKDQETGFMQRKVKRIHFVGIGGIGMSGIAEVLLNLGYGISGS
                     DVQSSDTTKRLQQLGAAVAVGHAAENIAGADVVVTSTAVKADNPEVLEAHRKNIPVIP
                     RAEMLAELLKMKFSVAVSGSHGKTTTTSMVSTLLAEGGLDPTMVIGGKLASIGSNARL
                     GGGDILVAEADESDGSFLKLSPTIAVITNIDREHLDYYPGIEEIKDAFLKFANMVPFY
                     GCTVMCVDNEHVRAIARSIKRRVISYGISQPADYTASDIRHSDAGTRYALSYRGEPLG
                     DVALMVPGLFNVYNSMAAIAVGRELGLEMATLCKGLLAFSGVQRRLEVKGEKAGITVV
                     DDYGHHPTEIMATLAAARQMRRGRLIVVFQPHRFTRTKALFDEFTRAFGDADVLVLND
                     IYPASETPIPGVHSAALWVAIKEGGHPCVEYIAQPQGTIEFLLANAKPGDTVLTLGAG
                     SVYKIGEAFLARLKEGTKNP"
     gene            18422..19348
                     /gene="murB"
                     /locus_tag="PLA58_03590"
     CDS             18422..19348
                     /gene="murB"
                     /locus_tag="PLA58_03590"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOR61909.1"
                     /translation="MTGHLKISAALQGANVGKVWFDEPISRHASLKLGGRVDALVMPE
                     SEVQLAELVRRLKENHLPFLPVGNLTNILVRDGGYRGALISLRGLDRAVCAPAEDGRF
                     RIEAQAGAALGRIVGLAADHELTGLEFCAGIPGSVGGAVWMNAGAFGSEIKDVLVFAD
                     LIDGEGKKKTLRREEIAFAYRKSNLPANVILCAACFSLSKGHPAQIRDRMAEIVKWRQ
                     EKHPLQYPSAGSVFKNLPGMPAGRLIEELGLKGRRLGDVQISKKHANFMINKGQGTAS
                     DMLKLIRLVQETVQKERGFFLETEIVVIGEES"
     gene            19345..20184
                     /locus_tag="PLA58_03595"
     CDS             19345..20184
                     /locus_tag="PLA58_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOR61910.1"
                     /translation="MSRKIKVSLEARKNRLRRRLIGALNESLGALGLLAVAVVISALF
                     IYLYGALLSAPALEVREISVRGVKELTEKDILQLANIQPRCNLLAVNTDAVARRIAAN
                     PWVKNIYVGRELPGRLVLDVRERKPIALVKQTGNFYLMDAGGFIFKKLSRTDDVDLAI
                     LTGVNLQNKPSPLLAEALKLLETLSADSYASLGTISEIHIDEVFGLSVLTDRGLHLKL
                     GRDNFTGKVQKLDIVLADLEKRGMKNGRLFVDLADISKVTVQRKDEPGKAQEQKKGPQ
                     YRI"
     gene            20209..21435
                     /gene="ftsA"
                     /locus_tag="PLA58_03600"
     CDS             20209..21435
                     /gene="ftsA"
                     /locus_tag="PLA58_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOR61911.1"
                     /translation="MGKKSNVLVGIDIGTTKTIAIVGEVKPAGIDIIGVGITPAKELR
                     KGGVVNIDSTVEAIKKAVEDAEHMSGCRINSAYVGIAGSHIKGQNSLGIVAVKGREVG
                     EDALQRAIEASKAIAIPVDREILHTLPQSYVVDGQDGIRDPVGMSGVRLEAKVHIVTG
                     AAASIQNVVKSVNRVGLDIEDVVLEQLAASEAILSPDEKDLGVALIDIGGATTGMAIF
                     AEGSIKHTATLPVGGNFLTTDIATGLRTPFAEAEKIKLNHGCAMTSMIPKEDVIEVPS
                     VGGREARMVSRQILGRIVEPRMDEILNMALKEIVRSGYEDLLAAGIVLTGGSSLLPGI
                     NEMAEQIFDMPVRRGCPSGVGGLSDAANSPAFAVGVGLIVYGSKNIPSDSVYRKTGNV
                     FGEYFKTLKKWFLEFF"
     gene            21454..22602
                     /gene="ftsZ"
                     /locus_tag="PLA58_03605"
     CDS             21454..22602
                     /gene="ftsZ"
                     /locus_tag="PLA58_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOR61912.1"
                     /translation="MFELTEECIENSARIKVVGAGGGGGNAVNTMISYTLRGVDFIIA
                     NTDAQALGASSAPVKIQIGAEVTKGLGAGSNPDIGKQAALESRDEIRRHLEGADMIFI
                     TAGLGGGTGTGAAPVIAEVARECGALTVAVVTKPFQFEGKRRNAQAEDGIVQLRDVVD
                     TLIVVPNQRLLSLGGRGLSLLDAFKKADDILYQAVKGISDLIIVPGLINLDFADVKNI
                     MSNMGMALMGTGTASGENRAVEAAQRAISSPLLEDNTIQGAQGILLSITGGPDMSLYE
                     VNEASSLIQAEAHEDANIIFGTVIDENMKDEIRITVIATGFEDARKKQAPSNVTHLGG
                     FRREDLSTPAFLRRSKSGDPANVVTMGAGDENENVDIDIPTFLRRQAD"
     gene            22661..24349
                     /locus_tag="PLA58_03610"
     CDS             22661..24349
                     /locus_tag="PLA58_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR61913.1"
                     /translation="MNWKEKKKYETLLARERGVVKKVWGTGLTVCLAYPNLYRIGMAN
                     LGFQTVYKIINELPSLLCERVFLPAGGDAEYAAGAVELLSLENQKPLRDFDVLAFSVS
                     FENDYPAILKMLALGGIPLCAKDRADGHPLVIGGGIALTLNPEPLADFFDAFLTGEAE
                     ETLPGFARMLAEARPSGPGREALLASLQKKMPGVYVPSLYEVQYFPDGRIRGMAPRLE
                     GLPEKIRAAPVKNIDAFCTTEVVSSPDAEMADMFLIEVNRGCPHFCRFCAAGHVYAPP
                     RFRSYEKITQAIDHGLKAKNKIGLIGTAVSDHPDLAKICRYIVDRQAQAGIGSLRLDK
                     VDESVVEMLRAGGIETVALAPEAGSQRLRDLLRKGITRDDILRAVRLLIDRDIANLRL
                     YFMAGLPTETEEDIDAVIELAKTIQHTALAHSKGKRKFRRITLSINQFIPKPHTPFQW
                     RPLGDVQEVGRRIKKITHAFRADKQINVIADVPKWAYVQALLSLGDRRVGGILAAVHR
                     LNGNWMRALKEVTINPDFYVYRQKDVNEVLPWDIIEAGLPKKVLIKEYQKALPD"
BASE COUNT         5323 a         6429 c         7549 g         5353 t
ORIGIN      
        1 gaagagagga gtggcattat gaagaaagta aattttctga tgcgttgttt tatcttgtca
       61 gtttcaggaa ttcttctggc tatgctggtt gtcggctgcg gctccctcgg cagcagtgta
      121 agcagcgcgc cgccggcttt aaaaggcgtt ttcatggatg gtccggtggg cgggattgcc
      181 tatgcgaccc cgtcgctcaa aggcgttacg aaagcggacg gcgtttttga gtacaggccg
      241 ggggaaacgg ttgcgttttc cgtgggcgaa ctggcgctgg gatccgcggc gggaaagccg
      301 gtggtcactg tgctggatct tgtgcctgac gccaaagatg cttccgacca gcgggtggtg
      361 aatatttgcg tgctgctgca gacgcttgac caggacggag accccgccaa tggcattctg
      421 atcagcgaac aggctgcgtc tttcgtcacg aaatacggca aagggacgaa ttttaatcag
      481 catatccgga gtttttcttt tgattccggt tttcgaagcc tcatggctga gctgaacaat
      541 gtggatgcgt tcggtgaaac ccccagagcc gtcgtacccg gcaaaattgc gcagaaacat
      601 ctggaagcga cgcttgccgg gctgaaaaaa tagaaaagca gcaaacaaaa aataaagatt
      661 tctttgccgt gtttgttaca gagggctttt gccgcgaggg gaaagccctc tctttgcctg
      721 acggcggcgc gcaggcggta cggtctactt tcctttgtac agatagcgct caaaaaaacg
      781 gttataggct gtccattccg agccggcgtt ggcggcgtcg gcctcgacaa aactatcaaa
      841 cgcgttgagt ttggcgtcca gatcatccat gaagtgtatg accagcgcct cccgggtttt
      901 cggacgtttg ggcgagccga attcaaattc gccgtgatgg ctcaaaatga tatggcgcaa
      961 ctcaagcgcc agccgttcag ggaaattgcc gatcgcctcc atttttttgt tgatcatttc
     1021 cacgcccatg accagatgac cgatcatccg gccttcgtcg ctgtattcga tgagcccgtc
     1081 gtaagaaaat tcataaattt tgccgatgtc gtgcaggatg ccgcctgcga tcagcagatc
     1141 ccggttcaat tgaggataac gcttcgcggc ttggtccagc aggcgcacta cggaaagcgt
     1201 atgctccagc aggccgccca ggtagatgtg gtgaaaccct ttggcagcgg gcgcccggcg
     1261 gaaaaggtcg gctgtttttt cgtcgttcag aaaagcgccc agcagctttt tcagaggatc
     1321 ggaagagatc gtatcgacat atgcgaggag attttcaaac attgccgcgg tattcagctt
     1381 gctgaccggc aggtagtccg atggatcgat atcctgcggg tcgcagggtt tggccgtgag
     1441 aatggaaatc tgaagggcat tgcggtaact ctgtgcgcgg ccttcaatga aaatgatgtc
     1501 gcctttctta aagacccggt ccagcgctac cgcgttgtcc cagaccttgc cgtcggtttc
     1561 ccccgttttg tctttgagcc ggacgttcag atacgccgac cctttctggg aaaaggccat
     1621 gctcttctcc gcaaccagaa aggaagaggc aattttgtcg ccgggctgga tgtcctgaag
     1681 atagatgtct ttggttttca aatgaaccgc ctttctaaaa aaatggagct taaggttttt
     1741 cccgcagaat ccggtggtct tccacacgaa ccgtcagttt gctggcgtca aaatattcca
     1801 tgagcgggat gatgtatttg cgcgacagtc cggttaagtc tttgaacgtc gccggcgtgg
     1861 cctgaccgtc ccggacaagc tgtgccttgt agtcctcacg caatttgtcc agcgccgtac
     1921 gcgcaaagca cagttcttca ttgattttta taagctcgcc gtctttgatc atcagccgga
     1981 cgatattttg cgccttgacc ttttgatcct taaatcgggt catcacatcg gaaagggaag
     2041 gcggcgccag tccggcctga agatagaccg atgcaagcga ttgccgtaaa gcgtcctctt
     2101 cccctgccag ctgcacctga tgggtcgcca accttacatg gtccttatcg ctggcaatcc
     2161 cttctttttt aatgaggcgg gcaagcacca tattgaaaag ttttccggat accgcgtccg
     2221 gaagcgccgc cttgagttct tccttggaga tgccttcttt taagggatgg ttcgcgtgat
     2281 aatccgcaag gcttttcatc aggcgctcct ccagatgagc gcacaggtcg gcggaaagcg
     2341 ctgtggcgtc gtcgcccgga agcaggatgg cccggcggtc ggaaaataat ttttccagcg
     2401 cttcccggat ttttttagcg gaaatcccca gacggaaggc gagggacgga acgttcaggc
     2461 cgttgaagcc ggcgcggtcc agcagaacgg aaattttgtc ctgcaggccg ccgctttgca
     2521 ggattttcaa atcgtccagt atctttttgt ttttcctttt gtgcttgccc ggctgcgggt
     2581 cgagaatccg accgccgccg atggtggtga tgggggagta gctgcgcagt acgaaatgat
     2641 cccctcccac caccacgtct tcattcgcca gaatcagctg ggcgaaggat tggtctccgg
     2701 gagaaagttc atcggcatcc agaagaacta ttcgcgtcat gatttccgcc gtgccggtgt
     2761 gcagacgcgc cagggaacgg tttttcagct tccgggcatt cccggccagg tattcaaaaa
     2821 acacatccag cctttgggtg ggccggacgg tctgcggtcg caccagaaca ttgccccgtt
     2881 cgagcgttga tttttcaatg ccctgaagat tgagcgcggt gcgctgtccg gaaaaggctt
     2941 cttccgtggg ctggttgtgc acctgaatgc cgcggatgcg ggcgaagata ttttccggca
     3001 gaatctgaac ctcttctccg acgcagattt tatcggaaat caacgtgccg gtgacgacgg
     3061 ttccgaagcc tttcatcgta aaaatcctgt caaccggcag acggaagatg ccgtcatcgg
     3121 ttttctcctg caggtggcgt acggttttgt cgatttcccc cagcagctca gggatgccct
     3181 gtgtttgaga tgctgaaacg ggcacaatgg gggcgccggc aagaaacgtg ctttgcagat
     3241 agtcggcgat ttccgagcgc accagatcca gccagtcttt ctccaccagg tcgattttgg
     3301 taagggccac caggccggtg gtaatgccca gaagcgagca gatttgcaga tgctccttcg
     3361 tttgcggcat gacaccttca tccgcggcga tgaccatcaa aaccagatcg atgcccgccg
     3421 cgccggccac catattccgg atgaactttt catgccccgg gacatcgacg atgcccagcg
     3481 tatggccgga gggaagcaaa agagaggcaa accccagttc gatcgtaatg cccctttcct
     3541 tttcctcctt gagacggtcc gtgtcaacgc ccgtgagggc cttgatcagc gatgtctttc
     3601 cgtgatctac gtggccggct gttcccaaaa cgaaatgttg catgtttctt cctgctggtt
     3661 cgcttttcaa gataggttca aataacaaac tcccgtcatt ttaacaacat gatattttta
     3721 caaacgtctg cgccgccgga acggatcatg atttacttct tgaaaatgca atgcggaatt
     3781 gttaaaaaaa caaacagagg tgatctttgc gcattctggg tgtggattat ggtgaaaaac
     3841 gaatcggttt ggccgtcagc gacgagctgg gcctgaccgc ccaggggctg cccacgctga
     3901 ttcgcaaaac caggaaaaat gatctcgaaa tcctgcggca gtggattcga aattattcgg
     3961 ttcagaaaat tgtcatcggc tatccgctgc gcatcgacgg cacggaaggc attcaatgtg
     4021 aaaaagtcaa ccggtttgcc cggtggcttg ctgaaagctt cctgctgccg gtgatgaaat
     4081 ggccggaaac cctgacgacc aaagaggcgg aagacattct gcgggcctcc ggcgtccgct
     4141 ggcagaagag gaaagaaaag gtcgatcagc tggcggcctg tctgattctg caaaattatc
     4201 tggacacagc cggaagggga gagcaagggt aagaacaccg gagttcgccc gctgtgtgcg
     4261 gtcttttttg agcggatgga atggctctat gaagtcaaag aaaagcaaaa taatgcttta
     4321 cgttttatcc ctgcccgtga tcgtggcctt ggtgtttggc gccatcctgt tcagctatgc
     4381 cgtgacgccg attcattcgg cgaaagaaaa aaccgttgtg gtggatattc cgaccggcag
     4441 cagcttgatt caaatcatca aaattctcca tgacgccgat ctggttcaaa accgattcct
     4501 gtttcatgtt ctggttacgg tgaaaggagg ggcccgatcg gttcgcgccg gagagtatga
     4561 atttgccgca acgattacgc cttcccatct ggcggacaag cttcttcatg gcgacatcaa
     4621 aaaatatcct gtaactattt acgaagacag ttcgctttcg caggttgccc tgcgtctcca
     4681 ggaaaacaaa ttgatcaacg aaaaggattt ttttgaatta tcgcaggatg cggaattttt
     4741 atccgagttg ggcatccgga gttcttccat ggaagggtat ctctttcccg atacgtattt
     4801 tctggatcga tccatgacca cgcggcggat catgcgcatg atggtggaac gatttcagag
     4861 caaggtctcg ccggaaatga tccgccaggc cgcgcaaaaa ggcttgaatg tccatcagtt
     4921 tgtaaccttt gcgtcactgg tgggaaaaga atccggctgt gctgcggaga agccgttgat
     4981 tgcagcggtt ttctataatc gtttgaaaaa ggggatgcct cttcagagtg atccgacggc
     5041 cgtttacgac ctcaagaatt tcaaggggaa agtgatgaga tcccactata aacgggaatc
     5101 tccttacaat acttacctga ttcgcggttt gccgcccggt cccattgcca gtccggggct
     5161 ggattctttt caggcggtcc tgcaacccgc agcggtggat tatctctatt ttgtttccaa
     5221 gggggacggc acgcaccact tttcgtcatc gcttatggcc cacaatgacg caacggttcg
     5281 ttttcgcaat atgattaaca ggaaagagaa agtggaagag acggttcgtt agccggtttt
     5341 tatttatcta tctgtataat ataattaatc tttattgtta aaatccggtt tttgcttctc
     5401 tcgtattcag gtacgttgca tttcccccct gcagctctgg ccggtttatt ttgcaatccg
     5461 tcaagtcgcc accgatttat aacccaattt tctcagtgat attcaattat tcgattatca
     5521 aaaaagattc attttttgct tgacaaagcg tttttccctc tttatagtcg ggccagtgga
     5581 gtaaagtggc ttattgtgga ggggaacgtg atgtccgtat ttcgtgggca ttatcaccat
     5641 actatcgacg aaaagggaag aattattttc ccgtccaggt tccgggatac cttcgccgag
     5701 cattatgaca ataaaatggt catcacgaac tgggacgggt atttaatcgt gttcccctac
     5761 gcccaatggc tgctcatcga agaagaagta ttgaggcggc gcattctgga caaaaaaatt
     5821 cgtgattttc aaagattttt tatgtccgga gcggtggact gcgctctgga cggtcaggga
     5881 cgggtgttga ttccgccggc cctgcgggaa tatgccaagc tggaaaaaga cattgttctg
     5941 gtgggcatgc tgaaaaatat tgaaatctgg cccagggaaa aatttgaaga aagaaacaaa
     6001 gaggccggcg agaaaataga cagggataac gagatcgccg agagcctggg gatatgaccg
     6061 tgacggccgg tttcgctcat gagccggtga tgcgcgacga agtgctgtcc tggctggtgg
     6121 gaaacaaaac cggggtttac gtggacggca cgattggcgg gggagggcac gccctcgcga
     6181 tcctggagaa cacccgggcg catctgatcg ggatcgaccg ggatgcggag gcgatccagg
     6241 ccgcccggca gaggctggaa ccttttcaat cgcgcgttac gcttgtcaag gggaactttg
     6301 ccgatctggc ctctgtgctg ggcgggctcg gcattgaaaa agtggacggc gtgattttgg
     6361 atctgggggt ttcctctcat caactggatg cggcgcacag aggattcagt tttggccggt
     6421 cggcgccgct ggatatgcga atggaccggg atgaaaagct tcgcgcttat gatatggtca
     6481 atggtttcac accgtccgaa ctggaaaagg tgatccggtt gtacggagaa gaaaaaatgg
     6541 cagcgagaat cgccagggca atcgtcagaa gaaggcaggc ttcgccgatc gaaaccacgg
     6601 cggaactggc ggccctgatc gcgcaggcga tgccgcaaag catgaagcat cagaaaattc
     6661 atcccgccac gcggactttt caggcgatcc ggattgccgt caaccgcgaa ctggacgctt
     6721 tggcttcggg cattcacgcc gccgtcgctg ccctttcggc cggcggccgt ctgggggtga
     6781 tttcgtttca ctccctggaa gaccggatcg tgaaacatac ctttcttgac ctggcggcgg
     6841 cctgtgtctg ccccaaagag gttccgtact gcatctgtca caaacaggcc gtcgtaaaag
     6901 ttttaacccg caaagccctg acgccttctc cggaggaaat ccggagaaac ccgcgggccc
     6961 gcagcgcaaa attgcgcgtg gcagagagga tttaagatgg cgcagacagc aggaacacag
     7021 gcgattacgc agggccggcc cctgaaagaa gccgatgccg cttccttcgg cgtgaagtat
     7081 ccgaccttta tcgttgtggc gcttgtcatc atgtttgtcg cggtgatcta tgtcggttca
     7141 cacatccgga tgacccaaat ggaatatgat gttgccgcgg cattgagcgc caaagaaaat
     7201 ttactggaag aacagaaaag attgaagctg gaactggcga tgctcaaatc gccgcagagg
     7261 attgaagaca tcgcccggac ggagctgcag atgacgtatc ccacggcggc gcaggtgatt
     7321 gttttaaagc aaacaaggaa gtgaccatga cggcagactc gaaaaaatgg ctgaaattcc
     7381 ggctggccag cattttggcg atttttttga ttctgttcat tgcgttgctt tcccgcgctt
     7441 ttcagctgca ggtcctgtcc ggcgagaagt tgaaaaaact ggccgtccgc cagcacatca
     7501 ctacgcttcc cattcattcc gaaagaggca tgatctatga ccgcaacggc gaaaaactgg
     7561 ccatgtcggt gctggcggac tctgtatgcg cggatccgac ccggatcagt gatccggcaa
     7621 gagtgtcgcg ggaaatcgct tccatcctga atatggaccc gtcggtcgtg ctgaaaaaga
     7681 tttcggagcc gaaaagtttc tgctggctgg ccagaaagat ttcttcccag caggcgctga
     7741 tggttgaatc cgcaaacctg gaagggattt ttctgatcaa ggaaccgaag cgtttttatc
     7801 cgaacggaca actggcggcg catttgatcg ggttttccgg atttgacgcc gagggcctgg
     7861 agggactgga aaaaaaatat gacccgtatt tgcgggggac gccggaaaaa ctgacctggg
     7921 ccagggacgc caagggcaaa aaactctttg tgcacgttga acatgatcct tccgccaaaa
     7981 gcgggagcgc caatctggtg ctgaccattg acagccgaat ccagtatctg gcggaaacgc
     8041 atttgaagga cgccgttctg gagaagggag cgaaaggcgg cgtggcgatt gtcatggatc
     8101 cgaaaacggg agagattctg gcgatggcca atgaaaaagg attcaatccc aacaacatca
     8161 aagggctgac gtcggaagcc tggcgaaacc gcgccatcac cgattctttt gatcccggat
     8221 caacctttaa gccctttctg gtcgccgccg ccctcgaaga aaaagtcgtc aaggagtcgg
     8281 accgctttta ctgcgagaat ggacattatc aggttgccaa ccgagtgatt cgggaagcca
     8341 accataaaaa gcatggttat ctggccgtgc gggacatcat taagtactcc agcaacatcg
     8401 gctcggccaa aatcgcggaa aagctcggcc gggaaaagtt ttatgcgtat attaaaaatt
     8461 tcggttttgg ctcccggacg ggcattgacc tgtcgggcga ggcggcgggt gtcttgcagc
     8521 ccgtcaggag ctggacgagg gtggatacga ccaacatcgg cttcgggcag ggcatttcgg
     8581 tcaccgccat tcaactcatc acagcgctgt cgtcgattgc caacggaggc gttctgatga
     8641 agccctatat cgtgaaaggg ctcaccgata aaaataacca gcccgtcaag atgttcgctc
     8701 cggaggccgt gcgccgggtg gtttctcccg atacggccag gcgcatggcg gttatgctga
     8761 ccgatgtggt cggggccgaa gacggcacgg gaaagagagc ccatattatg aatgtcgccg
     8821 tggccggaaa aaccgggacc gcgcaaaaat atgattttaa gcgcggcgct tactcctcgg
     8881 aaaaggtgcg gacgtcgttt atcggatttt tcccctcgga caatccgcag gtggtgattc
     8941 tggtcatgct cgatgaaccg cagcgggaca aatggggagg cgtggccgcc gcgccggtgt
     9001 ttaaaaacat cggcgagcag attctcaact gtttcaagac gaacattcgc gaagcccctg
     9061 cgtttgaccc ccagccggtt catgatctgc agttggtttc ggcggatgct tccctgatgg
     9121 aggatttggc ggttgagagc aatgagatcg atgatgaatc ccgcatgccg gattttaaag
     9181 gattaaccct tcgtgaggcg ttgaaaatgg cgaggtcccg gtcgatagag cttcgggtat
     9241 ccggcaacgg ctgggcgacc aagcaggttc ccgcgcccgg ttcggatctg ggcgaggatc
     9301 ggctgtgtaa agtttttttt gagatgaaga actgagggat gatgaagctt cgtcaattac
     9361 tgcagggagt ccggacggtc cggacggaaa cggatccgca aggcgatgtt tcatcgattt
     9421 gctatgtcgc agaggcgtgc ggcccggggt cgctctttgt cgcgattccg ggccttgcct
     9481 ttgacggcca ccattttatc ggccgggcca tcgagcgggg cgcgcgtttc attgtgcatc
     9541 agaaagacat cgaggttccc gacggggtca cggccgttca ggtatccgac agccgccgcg
     9601 cgctgggcac gcttgccaaa aattattttg gcgacccgtc ctcccgtctg accctgatcg
     9661 gcatcacggg gaccagcggg aagaccaccg tgtcctatct gctggaatcc atcctgacgg
     9721 ccgccggttt tcgctgcggt gttctgggga ccatcaatta ccgctacaac ggcaaattct
     9781 ttcccgctcc gaacacgacg ccggaatctt atgacctgca gagaattctg aacgagatgg
     9841 cggacgccgg ggttacgcat gtgattgccg aggtctcgtc gcacgcgctg gatctgaaaa
     9901 gagttgacga ctgcgacttt gatctggctg tttttaccaa tctcagtccg gagcatctgg
     9961 actaccacaa ggacatggaa gactatttcc gggcaaaaca aaggttgttt gccgagctgc
    10021 tgccccgaag ccggaaaaac cttccgtgta aagccgtgat caacgcggac gatccccggg
    10081 gacggcggct gatcggcgaa atttccgttc cggttctttc ctacggtctg gaaaatgccg
    10141 gcggggtgag cgcacagaac gcagttgttt cgctgcgcgg cattcgggcg gacatccgtc
    10201 tgaacgggga aaaaataccg gtgtcctccg ggctgatcgg cggattcaac ctgtccaata
    10261 ttctggcggc ttgtgccgct gcgtcggttc tgggggcgga acctcgggcc attgcggcag
    10321 gcattcaaaa cctgtcgttt gttcccgggc gtctggaaag ggttgttcct tcgggcggcg
    10381 ttcatgtttt tgtcgattac gcccacaagc cggatgccct ggagcaggtc ctccggaatc
    10441 tgcacggttt gaagcaaaaa agaatcctga cggttttcgg ctgcggggga aatcgcgacc
    10501 gcgccaaaag gccgatgatg ggaggcatcg cgacggaatg gagcgacctg acgattgtaa
    10561 cgtcggacaa ccccaggcgt gaagatccgc tggagatcat ccgtgaaatt gaagcgggga
    10621 tcgacgaaac caggatcaga aaagcatcgc cggagggcct ggaatttgac gccgccggcc
    10681 atgtctacac ggtgcttgcc gaccggagag ccgccatcga agccgctgtc cggatcgccc
    10741 aaccgcagga tatcgtcctg attgccggca agggccatga agattatcag attctgggaa
    10801 cgaaaaaagt gccgtttgac gaccggctgg ccgccgcacg ggctttgcgg ttgaagtttt
    10861 ccggacggat gtctccggcg ttttcgctgg ccgaggtgct ggccgcaacc ggcggacgcc
    10921 tggcggcggg agacgccgat caaaccgttt acggcatttc caccgattcc agacaaatcc
    10981 gccccggcaa tctttttgtc gcccttgccg gggagcattt tgacggacac gcctttgttg
    11041 caaaggccgt ggaagaagga gccgacggcg tcctggttgc cgatgccggc aaaatcaaga
    11101 tcgggacggc caatcagcag atcggcgtga ttgaagcggc ggatacgctg cgggcgctgg
    11161 gggatctggc ccatgcgcat cggcggcgtt tttccatccc cgtcattgcg ctgaccgggt
    11221 cttcgggcaa gaccaccacc cgcgaaatgc tggcctgcat tctggggcag gagaaaaacg
    11281 ttctccggac ggaggggaac ttcaataatc tgatcggcct tccacagacg cttttccgga
    11341 tgacggatca gcacgatgcg gccgttctgg aaatggggac caacacgcgc ggcgaaatca
    11401 aaaggctgac gcaaatcgcc gcgccggaca tcggtctgat caccaatgtc ggtcccgcgc
    11461 atctggcggg gttcggctcg gtggaggccg tgcgggagga aaaaggcgat ttatggctgc
    11521 acatggatcc ggcaggcact gccgtcgtga atctggacga tgaggcggtg cgccggacgg
    11581 ccgaccgatg gtccggacgg cgcgtgacct tcagcatgag cgccggcgcc gacgtcggcg
    11641 tgaacgacat ccgccgaaac ggcgcccggg gggtgagttt tcatctgctg atgaacgggc
    11701 aggcgcacaa ggtggacatg aaagttgcgg gccttcataa catttacaac gcgatggccg
    11761 ccgccgccgc ggcgttggcc tgcggcgccg gggccgaatc gatccgacgg ggcctgtcga
    11821 tgtttcagcc ggtcggcggg cgcatggaga tcctccggct gcaaaacggc gcgtatctga
    11881 tcaacgacgc ctacaatgcc aaccccgcct ccgtgcgcga agcgctgttg acgctcaggg
    11941 acctgatgaa ttcgcacgac gctttcgtgc tgctgggcga tatgctggaa ctgggcgacg
    12001 ccgcgccgga gatgcaccgg aagatcggcc tgctgttggc gaccatcggc gtggcggcgg
    12061 tttttcttca gggggatttt gcgcaagcaa ccgccgaggg cgcacgggaa ggcggactgg
    12121 ccgcggaacg gattctgttt ttacaagacg atgaggaagc cgtggctttt ttgaaaagga
    12181 agctgcgcaa aggcgactgg attctggtca agggctcgcg gcggatgaaa atggaccggc
    12241 tggcggcgcg gctgggcgaa gcaatcggcg ttgaaaaagc agaggaccag gaggcggccc
    12301 tgtaggcggg agaaaaaatg atttacgaat ttttatatcc tttgcacacg gtgttttcct
    12361 ccttcaacgt attccggtat atcacgttca ggaccatttt cgcctccatc acggccctcc
    12421 tgatctgtct ggcgctggga agatggttta ttgaaaagct gcagaaaatg cagattgatc
    12481 agcagattcg cgaggacgga ccccaaacgc atctggtcaa gaagggcacg ccgacgatgg
    12541 ggggcatgct gattctcttt gcggtgacgg tctccacgat cctgtgggcg aacctttccg
    12601 tggtgtatat ctggctggtg ctgctggtta ccgtcgggtt cggcctgatc ggctttctgg
    12661 atgattacag gaagctggct gttcggaatt cccggggcgt gcccgggaaa gtcaggctgg
    12721 ccgctgaaat catcatcgcc ctgttcgtcg gggtcgtttt gtatttcaag ccggggttca
    12781 gcgcgcaggt gacgattccc ttctttaaga cggttttgcc caatctgggc tggggctatg
    12841 tcctgctgtg cgttttcatc atcgtgggcg cggccaatgc cgtgaatctg acggacggcc
    12901 tggatggtct ggcgacgggg cccgcgggca tctgctttgt cacctatctg ctgttcgcct
    12961 attttgccgg caacgtcaag gttgcgcatt atctgcagat tccttatgtg cccggcgcgg
    13021 gcgagttgtc cgtgttctgc ggagcgttca tgggcgcggc gctgggcttt ctgtggttta
    13081 acgcccatcc ggcggaaatt ttcatgggcg atgtcggctc tctgtcgatg ggcggcgcgc
    13141 tgggaaccgt ggccgtcatc accaagcagg aaattcttct ggcgattgtc ggcggggttt
    13201 ttgtgatgga gacgttttcc gtcattttcc aggtcggtta tttcaagctg acgggcggga
    13261 aaagaatttt ccggatggcg ccgatccatc atcatttcga actcaagggc tgggcggagc
    13321 cgaaagtcat tgtccggttc tggatcattt cgatcctgct ggcgcttctg gcgatcagca
    13381 cattgaagct gcggtaggga aagcatgcaa ctggcagaca ggaaaatagc cgtgatcggc
    13441 atggggaaaa cgggcattgc cgccgccgcg tttctgggtg gaagcggagc cgccgtcgtg
    13501 gcggtcgatg agaaaccccg ggaccagtgg ggcggggagt ttgaacaaat cgcccatcgg
    13561 ccgtggcttg ccgtcggacg ctatgacgag acctgcctgg acggcgtgga tctggtggtg
    13621 ccgtctccgg ggatcccccc ccgccatccc cttctgacgg cggcgcagca aaaaaagatt
    13681 ccgatcttga gcgaggtcga gctggcctgc cggttcatcc gcgtgccgat catcgccgtg
    13741 acgggcacca acgggaaaac caccaccacc acgctgctgg gcgagattct ggcgcgcgcc
    13801 gggaaaaaag tgtttgtcgg cggcaacatc gggaatccac tgatcgaata cgccggcgcc
    13861 gcgcagacgg atgatttcat cgtcgccgaa atcagcagct ttcagcttca gtggatcgaa
    13921 tcgttccgtc cgcatgccgc cattctgctc aacatcacct gcgatcatgt caactaccac
    13981 gggtcgtttg aagaataccg ccgcatcaag gggagaattt ttgaaaacca gcgtgaaaac
    14041 gatctggcgg ttctgaacgc cgaagatccg cagcaggaag gcctggatgc ttcaatgcgg
    14101 gcctgcgtgg tccggttcag ctccgtccgg ccgcttgcgc cgggcctttg cctgcagaag
    14161 gattctctgg tgtacagcgg gcccgacggc acggatgagc ggtaccctct gggcatgatc
    14221 aggattcccg gtctgcataa tgtggaaaac gtcatggcgg cggtggccgt ggctcgcttc
    14281 tgcggctgtt caaaggagag cattgtggaa gcggtgtcgc agtttcgggg cctgccgcat
    14341 cggattgaat ttgccgggga aaagcgtgcg gttcaatttt atgacgattc caagggaacc
    14401 aatgttgatg ccgtcgtgcg ggcgctgcaa accttttcgt cgccggtgat tctgcttctg
    14461 ggcggacggg acaaggacgg aaattttgac gtgttgcagc cgatgctggg cgccaaaacc
    14521 aaacaggttg tcctgtttgg cgaggcgcgc gaccaaatcg agccgcagct gggcaatacg
    14581 acgccgatcg cgaaggaagc aacgctgaaa gcggccgtcc tccgggcgta ccgggacgcg
    14641 aaacccggag atattgtgct tctgtcgccg ggatgcgcga gctttgatgc gtttgccaat
    14701 tacagggagc ggggaaatca ttttaaggaa gtggtcagga atctttaaat ggacgctgcg
    14761 aaaaaagacg ccaatacgcc ggatgttatt cttttgctgg tcaccctgat actggtgacc
    14821 gtgggcacgg cgatgattta ttcgtcgagc tccattctgg cgatggaaaa attcaaagac
    14881 ggacaattct ttctcaaaaa gcatttattt tttgtattcg ccggcctggt gatcatgatc
    14941 gttatgacca aaattccata cgaacagctc aagaagctgg cgtaccccgg cgtgatcgtc
    15001 tccgtggtcc tgctgctgtt gctttttatt ccgcacgtcg gcgtgaagcg gggaggcgcg
    15061 acccgctggc tcaatctggg ggtgttttcc tttcaggtga ccgaactggt caaggtggcc
    15121 atggtgcttt tcctggcgca tctgctgacg agaaaggcgc gccatttaaa aaaattcagc
    15181 cggggcgttc tggttcccct gtcggtgacg gccgtggtca tgggactgat cattcttcag
    15241 ccggatttcg gcacggtcgt tattatcatg tcgattctgc tgttgatgct gtctctggcc
    15301 ggatcccgga tcacccatct ggtgtttctg ggcgcggcct ttattcccgt gggcatctgg
    15361 ctgatcatga gcaaggggta ccggatggcg cggctgacgg ccttcctcga tccctggaaa
    15421 gacgcggaca acaccggttt tcagctcatc cagtcgctga tttcctttgg atcgggcggc
    15481 gtgacggggc agggcatcgg cgacggcatg cagaaattgt tttatctgcc ggagccgcat
    15541 acggatttta ttctggcggt gattgcggag gaaagcggtt ttatcggcgt gacgatcatt
    15601 attttcatgt tcattttctt tctgctgcgg ggctttatga tcgcttttcg cgcgccgaat
    15661 ctctttggca cgctgctggc cgccggtctg acgatgctga tcacgctgca ggccttcatt
    15721 aacatcgcgg gggtgatggg gctcattccc ctgaaggggc tcgcgcttcc attcttgagt
    15781 tacggcggta cgtcgtttgt catgagcatg atgaccgtgg ggattctgct gaacatctcc
    15841 acgcagagga cctgagcggc gaaaagataa agaggagcaa agcaggcgcg caggcggggg
    15901 aaaaccggtg aggattatca ttgcgggagg aggaacgggc ggtcatctgt ttcccggcat
    15961 tgccgtcgcg gaggagtttc agaggcgcga cgctcaaaac cgggtgatct ttatcggaac
    16021 cagcaggggc attgaatacc ggcttttgga aaagatggga tacgaactga agctcattga
    16081 cgtggaaggg ctgaagggaa ggggttggaa ggcattgatc aaaagcgttt acgcgatccc
    16141 ggtcagcatg atgcagtcca ggcggattct cgcggatttc cggcccgata tcgttttggg
    16201 cgtcggcgga tatgcctcgg gtcccgccgt catggccgcg tatctgacgg gttgtcccac
    16261 cgccattgcg gagcaaaacg ccctggcggg aaacacgaac cgcattctgg ggaaatttgt
    16321 cgatcggata tttctgacct atgagcggag caaaggaaga tttcccgccc ggaaagtccg
    16381 tgtgacgggc aatccggtcc gggcggcgtt tgccgcgcgt ctgaaggaag ccccgaagac
    16441 gcgcgctttt cggcagattc tggtgttcgg cggttcgcag ggagcggcgg ccatcaacaa
    16501 aacggtcgtg gccatgctgc cgatgctgca aaagatcaaa gaccgggttc gggtggtcca
    16561 ccagaccgga gacagagacc tggcgatggt ccggcaggcg tatgaacagg ccggcgtgga
    16621 agccgatgtt caaccgttca tcgtcgatat ggcgtcggcc tatgcggcgt ccgacctgat
    16681 catctgccgg gccggagcga cgtcgctggc ggaaatcacg gcggcgggaa aggcgtccgt
    16741 gctgatcccc tttccgtacg cggccgatga tcatcagacc gaaaatgcgc gggcaatggt
    16801 cgaagccggc gcggcggcca tgatccggga gagcgatctg accgccgggg ccctgtattc
    16861 gctggtggcg tccctgctgg gcgacgacaa taaattacgg ggcatggaag agaaatccaa
    16921 agcgctgggc aggcccgatg ccgctgccag aatggtcgat gagtgcatca agctggcggc
    16981 ggaaaaccaa cattcaacag gataaaagga aaatgagcaa agaccaggaa accggattca
    17041 tgcagcgtaa agtcaagcgc atccattttg tcggcatcgg cggcatcgga atgagcggca
    17101 tcgccgaagt gctgctgaat ctcggatacg gcatcagcgg atcggacgtc cagtcctccg
    17161 acacgacgaa gcgcctgcag cagctgggcg cagccgttgc cgtcgggcat gcggcggaaa
    17221 acatcgccgg cgcggatgtg gtggtcacat cgaccgccgt caaggcggac aatccggaag
    17281 ttctggaagc gcatcgcaag aatattccgg tgattccccg ggcggagatg ctggcggagc
    17341 tgttaaagat gaaattttcc gtggccgttt ccggcagcca cggcaagacc acgaccacct
    17401 cgatggtttc gacccttctg gcggagggcg gactggatcc cacgatggtg atcggcggca
    17461 aactggccag catcggatcc aacgcccgtc tgggcggcgg agacattctc gttgcggagg
    17521 cggatgagag cgacggatcg tttttgaagc tgtcgccgac gatcgccgtc attaccaata
    17581 tcgaccgcga gcatctggac tattatccgg gcattgaaga aatcaaggac gctttcctga
    17641 aatttgccaa catggttccc ttttacggct gcacggtgat gtgcgtcgac aacgagcatg
    17701 tgcgcgccat tgcccgctcg atcaaacgcc gcgtgatttc gtacggcatt tcacagccgg
    17761 cggattatac ggcgagcgac atccggcatt ccgacgccgg aacccgctat gccctgtctt
    17821 acaggggcga acccctgggc gacgttgcct tgatggtgcc gggcctcttt aatgtttaca
    17881 actccatggc cgccattgcc gtggggcgag aactgggtct ggagatggcg accctttgca
    17941 aaggactgct ggctttttcc ggcgtgcagc ggcggcttga agtcaagggg gagaaggccg
    18001 ggatcacggt ggtggatgac tacggccatc acccgacgga aattatggcc acgctggcgg
    18061 cggcgcgcca gatgcggcga ggccggctga tcgtcgtgtt tcagccccac cgctttacgc
    18121 ggaccaaagc gctgttcgac gaattcacca gggcgttcgg ggacgcggac gtgctggtgc
    18181 tcaacgatat ttatccggcg tcggagacgc cgattcccgg cgttcattcc gcggcgctgt
    18241 gggtggcgat caaggagggg gggcatccct gcgtggaata catcgcccag ccgcagggca
    18301 cgatcgaatt tctgctggcc aatgcaaagc ccggggacac cgtcctgacg ctgggcgcgg
    18361 gcagcgtgta taaaatcggc gaggcgtttc tcgcgcggct caaagaggga acgaagaatc
    18421 catgaccggt catttgaaaa tttccgcggc gcttcagggc gcgaatgtcg gcaaggtatg
    18481 gtttgacgag ccgatatcgc gccacgcttc gctgaaactc ggcggccggg tggacgcgct
    18541 ggtgatgccc gaaagcgaag tgcagctcgc ggaacttgtg cgcagactga aggagaatca
    18601 tcttccgttt ctgccggtcg ggaatctcac caatatcctc gtgcgcgacg gcgggtaccg
    18661 cggcgccctg atctcgctgc gcggcctgga cagggccgtg tgcgcgcctg cggaagacgg
    18721 gcggttccgg atcgaagcgc aggcaggggc cgcgctgggc aggattgtcg ggctggccgc
    18781 cgatcatgaa ctgacgggtc tggagttttg cgcggggatt cccggcagcg tcggcggggc
    18841 cgtctggatg aacgccggcg cgttcggatc cgaaatcaag gacgtgctgg ttttcgcgga
    18901 tttgatcgac ggcgaaggaa agaagaaaac cctgcggcgc gaagagatcg cgtttgccta
    18961 ccgcaaatcc aatttgcccg caaatgtcat tctctgcgcg gcgtgctttt ccttgtcgaa
    19021 aggccatccg gcgcagatcc gggatcggat ggcggaaatt gtgaaatggc gtcaggaaaa
    19081 acatcccctg cagtatccca gcgccgggtc ggtttttaaa aatcttccgg gcatgccggc
    19141 gggccgactg atcgaagagc tgggcctgaa gggccggcgg ctgggcgacg tgcagatttc
    19201 gaagaagcat gccaacttca tgatcaacaa agggcagggc acggcgtccg acatgctgaa
    19261 gttgatccgg ctggtgcagg aaacggttca aaaggaaaga ggttttttcc tggagacgga
    19321 aattgtcgtc atcggagaag aatcgtgagt cgaaagataa aagtcagtct ggaagccaga
    19381 aaaaaccgcc tgcgacggcg tctgatcggg gcgttgaacg aatcgctggg ggctttgggc
    19441 ctgctggctg tcgccgttgt catcagcgcc ctgtttattt atctttacgg cgcgctgctg
    19501 agcgcccccg cgctggaagt cagggaaatc tccgttcgcg gagtgaaaga gctgacggaa
    19561 aaggacatcc tgcagctggc gaacattcag ccgcgctgca atcttctggc ggtcaacacg
    19621 gatgccgtgg cgcggcgaat tgccgccaat ccctgggtca aaaatattta tgtcggccgc
    19681 gagctgccgg gtcggctggt gctggacgtg cgcgaacgga agccgattgc gctggtcaag
    19741 caaaccggga atttttacct gatggacgcg ggaggcttta tcttcaaaaa attaagccgg
    19801 acggatgatg ttgatctggc gattctgacc ggcgtgaacc ttcagaacaa accgtccccc
    19861 ctgctggccg aagcgctgaa gctgctggaa accctgtccg ccgattcgta tgcgtctctg
    19921 gggaccattt ccgaaattca tatcgacgag gtgttcggcc tgtccgtcct gaccgaccgg
    19981 ggactgcatt taaagctggg gcgtgacaac tttacaggca aggtgcaaaa acttgatatt
    20041 gttctggcgg acctggaaaa gcgggggatg aagaacggcc gtttgttcgt ggacctggcc
    20101 gacatttcca aggtgaccgt tcagcgaaag gacgagccgg gaaaggccca ggagcagaag
    20161 aaaggaccgc agtacagaat ataggcagtg acggggaagg gtgagattat gggaaagaaa
    20221 agcaacgttc tggtgggaat tgatattgga acgaccaaaa ccattgcgat tgtcggcgag
    20281 gtaaaaccag ccggcattga catcatcggc gtggggatca cgcccgccaa ggaactgcgc
    20341 aaggggggtg tggtcaacat cgacagcacc gtcgaggcga tcaagaaggc cgtggaggac
    20401 gccgagcaca tgtccggctg ccgcatcaac tccgcctatg tcgggattgc cggcagccac
    20461 atcaaaggac agaactcgct gggcattgtc gccgtaaaag gccgggaagt cggcgaagac
    20521 gccctgcaaa gggcgattga ggcttccaag gcgatcgcca ttcccgtgga ccgggaaatt
    20581 ctgcatacgc tgcctcagag ttacgtggtg gacggacagg acggcatccg cgatccggtg
    20641 gggatgtcgg gcgtgcggct ggaagccaag gtgcatatcg tgacgggcgc cgccgcttcg
    20701 attcaaaatg tggtcaaatc cgtcaatcgc gtgggcctcg acatcgaaga tgtcgtgctg
    20761 gagcagctgg cggccagcga ggccattctc agccccgatg aaaaggacct gggcgttgcc
    20821 ctgatcgaca tcggcggggc gactacgggg atggccatct ttgcggaggg aagcatcaag
    20881 cacacggcca cacttccggt gggcggcaac ttcctgacca cggacatcgc cacgggtctg
    20941 cgaacgccgt ttgccgaggc ggagaaaatc aagctgaacc acggctgcgc catgacgtcg
    21001 atgatcccga aggaagacgt tattgaagtg cccagcgtgg gcggccggga agcccggatg
    21061 gtgtcgcggc agatcctggg ccggattgtg gagccccgca tggatgaaat tctgaatatg
    21121 gcgctcaagg aaatcgtgcg ttccggatac gaagacctgc tggcggcggg cattgtgctg
    21181 accggcggct cgtcgctttt gcccggcatc aacgaaatgg cggagcagat ttttgacatg
    21241 ccggtgcgcc gcgggtgtcc ttccggcgtc ggcggtttga gcgatgcggc caactcaccg
    21301 gcctttgcgg tcggcgttgg tttgattgtt tacggcagta aaaatattcc cagcgattcc
    21361 gtctatcgga aaacggggaa tgtattcggc gaatatttca aaacactcaa aaaatggttt
    21421 ctggaatttt tctaaacaaa gaagggggtg tgcatgtttg aattaacgga agaatgcatc
    21481 gagaattcgg caagaatcaa agtggtgggc gcgggcggcg gcggcgggaa cgccgtcaat
    21541 accatgatct cctatacgct gcggggcgtc gattttatca tcgcgaacac ggacgcccag
    21601 gcgctgggag cgtccagcgc gcccgtgaaa atccagatcg gagcggaggt gaccaagggc
    21661 ctgggcgcgg gctccaatcc ggacatcggc aaacaggcgg cgctggaatc ccgagatgaa
    21721 atccgccggc acctggaagg ggcggacatg atcttcatca cggcgggcct cggcggaggg
    21781 accggcacgg gcgcggcgcc ggtgatcgcg gaggtggccc gggaatgcgg cgcgctgacc
    21841 gttgccgtgg tcaccaagcc ctttcagttt gaaggcaaaa gaagaaatgc gcaggcggaa
    21901 gacggcatcg tgcaactgcg cgacgtggtc gacaccctga ttgtggttcc caaccagagg
    21961 ctgttgagcc tgggcgggcg ggggctttca ctgctggacg ccttcaagaa ggccgatgat
    22021 attctctatc aggccgtaaa aggcatctcc gatctgatca ttgtccccgg tctgatcaac
    22081 ctcgactttg ccgacgtcaa gaacatcatg agcaatatgg gcatggcgct gatgggcacc
    22141 ggcacggcca gcggcgagaa ccgggccgtc gaggcggccc agcgggcgat ttcctcgccg
    22201 cttttggagg acaacaccat ccagggcgcg cagggcattc tgctcagcat caccggcgga
    22261 cccgacatga gcctgtacga agtgaacgag gcctcgtccc tgattcaggc cgaggcgcat
    22321 gaggatgcca atatcatctt cggcacggtc attgacgaaa acatgaagga tgaaatccgc
    22381 atcaccgtga ttgcgacggg ctttgaggac gcccggaaga aacaggcccc ctccaacgtg
    22441 acgcatcttg gcggcttccg gcgggaagat ctgtcaacgc cggcgttcct gcgcaggagc
    22501 aaatccggcg acccggccaa tgtggtgacg atgggcgcgg gcgacgaaaa cgaaaacgtg
    22561 gatatcgata ttcccacgtt cctgcgccgg caggcggact aggaaaacat cgtggctgtc
    22621 cggatgcggg ctggagcgcg cgggaggcca tgaacggtcc atgaactgga aggaaaagaa
    22681 aaaatacgag accctgctgg ctcgtgagcg gggcgtcgtg aaaaaagtct ggggaaccgg
    22741 cctgacggtt tgcctggcct atcccaatct ttaccggatc ggcatggcca atctcggctt
    22801 ccagacggtt tacaaaatca tcaacgagct cccctccctt ctttgtgaga gagtgtttct
    22861 gcccgccggc ggcgacgccg aatacgccgc cggcgcggtg gaactgctca gcctggaaaa
    22921 tcaaaagcct cttcgtgatt ttgatgtcct ggcgttttcc gtttcctttg agaacgatta
    22981 cccggccatc ctgaaaatgc tcgccctggg cggcattccg ctctgcgcga aggaccgcgc
    23041 ggacggtcac ccgctggtga tcggcggcgg catcgcgctg accctcaatc cggagcccct
    23101 ggcggatttc ttcgacgctt ttctgaccgg agaagccgaa gagacgctgc ccggatttgc
    23161 gcggatgctg gccgaagccc ggccgtccgg ccccggccgg gaagccctgc tggcgtcttt
    23221 gcagaagaaa atgccggggg tgtatgttcc ttctctttat gaggttcagt attttccgga
    23281 cggccgaatc cgcggcatgg cgccgcgcct ggagggcctg cctgagaaaa tcagagcagc
    23341 cccggtcaaa aatattgacg ctttttgcac gacggaggtt gtcagcagcc ccgacgcgga
    23401 aatggcggac atgtttctga ttgaagtcaa ccgcggttgt ccgcatttct gccgtttctg
    23461 tgcggcggga cacgtttatg ccccgccccg tttccgcagc tatgaaaaaa ttacgcaggc
    23521 cattgatcat ggtctgaaag cgaaaaataa aatcggtttg atcggcacgg cggtttcgga
    23581 ccatcccgat ctggcaaaaa tctgccggta tattgtggac cgccaggcgc aggccggcat
    23641 tggctcgctg cgcctggaca aggttgatga aagcgttgtg gagatgctca gagccggcgg
    23701 catcgagacg gtcgccctcg cgccggaggc gggaagccag cgtctgcggg atctgctgcg
    23761 caagggcatc acccgggatg atatcctgcg ggccgtgcgg cttttgatcg accgggatat
    23821 tgccaatctc cggctgtatt ttatggcagg gttgcccacg gaaacggaag aagacatcga
    23881 cgccgtcatt gagctggcca aaaccatcca gcacacggcg ctggcccact caaaaggaaa
    23941 acgaaaattc cggcgcatca cactcagcat caatcaattc atccccaaac cgcatacgcc
    24001 gttccaatgg cgcccgcttg gggatgttca ggaggtgggg cgcagaatca aaaagatcac
    24061 ccacgcattt cgtgccgaca agcagatcaa cgtcattgcc gacgttccga agtgggccta
    24121 cgtgcaggcg cttttgtccc tgggggaccg gcgcgtcggg ggaattctcg cggccgtcca
    24181 tcgcctgaac ggcaactgga tgcgggcgct caaagaggtg accatcaatc ccgacttcta
    24241 cgtctatcgt cagaaggatg taaatgaagt tctgccgtgg gatattatcg aagccggcct
    24301 tcccaaaaag gtattgatca aagaatatca aaaggctctg ccggattgat cttgcctccc
    24361 gttcaaagga cttccggaac catgattcct ggtcaaggat tgatatattg tccgaggcta
    24421 tttacgatgt ctttgccccg attgaggctg tcggttttat gcctgaagag tgggacaaac
    24481 aaacatcgcc gctctatgat tacgcaaagc agggtgaaat tttatacgcc gcatgacaat
    24541 gtcgatgtta tgatgattct ccttattttc attgaggaag gccataaggt aacagtgtcc
    24601 cgttcagtct tctgaggttt ttagtaaatc acaaaattgt ctgttcattt tcaa
//
LOCUS       DAOSRM010000021        24079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2273_length_24079_cov_100.329004, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000021 DAOSRM010000000
VERSION     DAOSRM010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 24079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24079
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2273_length_24079_cov_100.329004"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLA58_03615"
     CDS             complement(<1..373)
                     /locus_tag="PLA58_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOR61914.1"
                     /translation="MADKSKVGMVFPPYIFEVEKGKIAEFAMAVSQKESKSQVKQIYA
                     DSGAAKAAGYKDIVPPSTFQTCFLLWGGGGLMPMLQEVGINLVRLLHGEEEYEYLLPI
                     HPGDVMTGVIKVVEMFDKEKKG"
     gene            complement(419..1600)
                     /locus_tag="PLA58_03620"
     CDS             complement(419..1600)
                     /locus_tag="PLA58_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOR61915.1"
                     /translation="MAGPLEGLKVLDFTTLLPGPYATMILSDLGADVLKVTSGSRPDL
                     AAIVPPFIGKTDLTAASAYLGRGKRSITLNLKDARGLQVVHRLLERYDVLIEQFRPGI
                     MAKLGLGFDDLAPRYPSLIYCSLTGYGQTGPMKDRAGHDINYLSLSGLMGYAGRKYEG
                     PVPMPLQIADVASGSNNAVIAILAAVFSRSRSGKGQYLDISMTDGAFAFNALAAAAYL
                     VSGEEPTREGFILNGGSLYDFYKTADGHYLSFGGLEPQFFAAFCNAIGRPDWIAGGVA
                     PPDCAGVKEEVRKIIKSKTRGEWKEIFDKTDACVEPVLSLSEALDSELVRERQLTTDI
                     TLPDGSTVRQPASPFKFSATPPVYGKTGVKAGSHNREVFLELGYSEEEINEFGKTGLF
                     S"
     gene            complement(1604..2746)
                     /locus_tag="PLA58_03625"
     CDS             complement(1604..2746)
                     /locus_tag="PLA58_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR61916.1"
                     /translation="MDIIQYTDEHRMFRDTFRKFVANELLPHVEEWEEAGIVPKSAWK
                     KMGEHGFLGMSVPVEYGGAGADFLYSVIVIEELSHSNFAGLAAPLHTDIIVPYIISFG
                     SEDQKQKYLPGCVTGDIITAVAMTEPNTGSDLAAIKTTAVEDGNDIVLNGQKTFISNG
                     INCDLCVVAAKDPACTDPHKAVDLFVVEAGTPGFEKGKKIKKAGWHSQDTSELYFTDC
                     RIPKENRLGEKGTGFLKLMMKLQQERLVVAISGIAAAEYMLETTIQYCRERTVFGKPI
                     SKFQVNQFTLVECATEIKIGRTFVDKLIVDHMEGRQVVIETSMAKYWITDMAHQVADR
                     CLQLHGGYGYCDEYGISRAWRDSRVSRIFAGTNEIMKTIIAKFMGL"
     gene            complement(2888..4102)
                     /locus_tag="PLA58_03630"
     CDS             complement(2888..4102)
                     /locus_tag="PLA58_03630"
                     /inference="COORDINATES: protein motif:HMM:NF004810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOR61917.1"
                     /translation="MASGIRDKVAIIGMGCSRFGERWDVGAEGLMVEAFQEAIADAGI
                     EKKDIQAAWYGVCMDEVNVGKGAPSMGVALRLPNIPISRMENYCATGTEAFRGAVYGV
                     ASGAYDICLALGVEKLKDTGYGGLPAWGSTAGTLMWQFFPNATAPGLFAQLASAYVAK
                     FKIKNDDIKQAIARVSVKSHANGALNPKAHLRKAVTIDQVLAAPIIAWPLGLFDCCGV
                     SDGSACAIVTTPEIAQKLGRKKKDLVTVKAVQLAVSNGYEGGFNEWDGANFVTTDKCA
                     AATYKEAGITNPREEISMFECHDCFSITELVTYEDLHLSERGHAWKDALDGMYDRDGK
                     IPAQIDGGLKCFGHPIGASGLRMIYEMYLQLQGRADKRQLENPRYGLTHNLGGFPHQN
                     VCAISIVGKYGK"
     gene            complement(4118..6835)
                     /locus_tag="PLA58_03635"
     CDS             complement(4118..6835)
                     /locus_tag="PLA58_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR61918.1"
                     /translation="MIGITSYGAYIPRLRLDRMAIYQSMGWFAPALISVAQGERSMCN
                     YDEDSLTMAVAASMDCLIGKDKTKIDAGYLCSTSLPFLDRQNAGILATALNLRSDINT
                     ADFTSCQRAGTTGLLTALDVVKSGSRKSILVTASDKRQTRPAGFYEMWFGDGAASVLV
                     GDEDVVAEFLGSYSLTYDFVDHYRGSKSEFDYTWEERWLREEGYSKIIPEAVNGLFKK
                     LNISMNDVQKLVFPCLFKAEHRAIGKKLGAAPEKLADTMHEVTGETGVAHALLMLVGA
                     LETAKPGDGILLAGFGQGCDALYFKVTENIGKLAERAGLKGSLANKKTTDNYMKFLKF
                     RDLINPEMGIRSEAPVQTAMTALWRNRKMILGFVGGKCRDCGTAQYPPADLCVNPACG
                     HLKTQDDYEFANIKARVRSFTGDLLAVSVDPPAIYGMVQFEGGGRLMMDFTDSELNDI
                     KVGMPVKMSFRRRVVDTVRGFSQYFWKAVPQTDWIPEIDFQGRVAIVTGAGGGLGRAY
                     ALELGRRGAKVVVNDFGGARDGSGAGAAGPADLVVADIKAMGGEAVANYDNVATPEGG
                     RKIVKTALDAFGKVDILINNAGILRDKGILKMEPENWRAVLAVHLNGAYNVTRPAFEA
                     MRQNGYGRIIMTTSAAGLYGNFGQTNYSAAKLGLVGFMNTLKLEGAKYNIKVNTIAPI
                     AASRLTEDMMPPEMLEKMNPAYVAGIVLYLCSEDCKDSGNIFNAGAGFFSRAAILTGP
                     GVMLGDETHVPTPEMIRDNWDKINSLEGAKPYTELNMAIMDFLTPPAAGAPAPESGAS
                     GLDVAGVFKKMPSVFNAAAAAGKDVTFQFNISGPAGGDWFVAVKDGTCSVTTGKGDNP
                     TTTIGMADTDFVDLITGKLDGMKAFSAGKLKVGGDMMKSQLIGKLFKFN"
     gene            complement(6906..8183)
                     /locus_tag="PLA58_03640"
     CDS             complement(6906..8183)
                     /locus_tag="PLA58_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR61919.1"
                     /translation="MMGASKEIRFDGRVAVVTGAGGGLGRVYALEFARRGAKVVINDF
                     GGARDGAGPGAAGPADKVVDEIKALGGEAVANYDNVATANGGENIVKTAVDAFGKVDI
                     LVNNAGILRDKGILKMEPENWQAVLDVHLNGAFHVTRPAFAVMRDHGYGRIIMTTSAA
                     GLYGNFGQSNYSSAKLGLVGFMNTLKLEGIKYNIKVNTVAPIAASRLTEDILPPDLLA
                     KMNPAYVAPIVLYLGSEECNETGRIYNCGAGFYNRAAMMTSPGVVIGSGKKVPSVEDI
                     SVNWAKIMDLKGAKEYGQLNDLVLQDLLSALEGKKEPAQSGGGFAAVKDVFEKMPGAF
                     QAGAAGGAAVVFQYCISGDGGGDWSVDIKNGACTIASGLHASPTCTLKIQAGDFLDLM
                     NGKVNAMKAYTSGKLKIEGDIMKSQLLEKIFKF"
     gene            complement(8258..9241)
                     /locus_tag="PLA58_03645"
     CDS             complement(8258..9241)
                     /locus_tag="PLA58_03645"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOR61920.1"
                     /translation="MKTAITEMFGIKYPIICGAMMWLCKPTLAAAVSNAGGMGNLTAG
                     NYETEEDFRNAIRQTRKLTDKPFMVGITILPSVRITAGHHQMYLRVCAEEKVAGIEVS
                     GAPVDKAVGPEYIDLLKKAGVKLFHKVGSVRHALHAEKAGYDGIYAAGIEEGGHPLDD
                     DVTTMVLTPRIVESVKIPVVTVGGIANGKSLAAALTLGAQGVMMASRFIATKDCVVHN
                     NIKEEIVKRQEYETTLICKTLHLQGRALKNQVVKNICEIEEKGGGFEALYPLIAGERM
                     KEAWEDGDVEVAPMMVGQSIGLIQDVPTCKELLARMVKEAEETLARVNKLF"
     gene            complement(9328..11196)
                     /locus_tag="PLA58_03650"
     CDS             complement(9328..11196)
                     /locus_tag="PLA58_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOR61921.1"
                     /translation="MQKYLVDHRDLRFTLFEFLEVDKMNRFRRFENFDGDVYEETIRL
                     AEKIAAQWVFPANVTGHREGCRYDPQTRSVRLPSGYSDAARALCDAGFLSIADDQEIG
                     GAGMPLAIQSCTWEIFCGAGGALMLFFMLGHGAAHLIRSFGTPEQKSLYVQKLLSGEW
                     GGTMCLTEPEAGSDVGALKTKAILQPDGTYRITGQKCFITNGDQDITRQIIYPVLARI
                     EGDPPGTRGISIFIVPKYLVREDGSLGERNDVFCTGVEHKMGAHGSATCAMSFGDNEN
                     CTGYLLGERGRGMNIMFQMLNETRLEVGIMALGGSSAALQYAVDYARTRLQGVHFSKM
                     FEAAAPKVPIIEHPDVTRMLMGMKALVEAQRMLSYYAAMQIDLSNLLEGEASREARSI
                     VDILIPLIKAGNSENAWQITAEAIQVHGGYGYCSDYPVEQLAVDCKVLSIVEGTNGIQ
                     SLDLVMRKILLNADRKNYKVLKSRIYQTIENASGIVDDVYRNMLLEGLQRMDSILDTM
                     KKHMDEGRYHTLLLNVDPLRRAFFDLMLAWMHLWCLTLARPKLSALTAGAKGEALTEV
                     LARDAEAAFYYGKVCASEFWLATEFPKYFGKMEGISLGETVDFLPGNAVFSSHPCA"
     gene            complement(11286..12089)
                     /locus_tag="PLA58_03655"
     CDS             complement(11286..12089)
                     /locus_tag="PLA58_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOR61922.1"
                     /translation="MSTLMPEFKTLLFEEKEPRIGVVTMNRPGQLNAINLDMLGDFER
                     LFEVLSGEDSIRVLILTGAGKGYSSGADLNDAVIYKESEAFADPERFLKFVQERYAAL
                     ILGMRRIPQPIISAVNGPAAGGGFSMTLASDIRVACKEAFFVASFINIGLSGGELGCS
                     FLLPRLVGLAHASDILFTGRKIGGEEAERIGLVNKLVPREDLLEAALFYARSMVGKSE
                     GGLKLTKRVLNENLHAPSLEAAVNLENRNQTIMVFSGEFFKLVQKFYKA"
     gene            complement(12086..13765)
                     /locus_tag="PLA58_03660"
     CDS             complement(12086..13765)
                     /locus_tag="PLA58_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOR61923.1"
                     /translation="MSFERIWHKSYASGVPAEITVEKITMAEALTRTAATYPERVAMI
                     YMGKKITYRQLEKLVNRFTRALIDIGVREGDKVAMLLPNIPQMIIADHATYRAGAATA
                     MNNPLYTERELTHQLDDSDATVLVTLDLLLPRALKIKSETKIKKIITCHISDYLPFPK
                     KQLFPYVKKAMFRKVEPQPDVYEFMDLIARYPDTPVENKAKWDSLAALIYTGGTTGVS
                     KGAMLTHANISSVVQQFSVWFPDLKGQYQNLTGIYPVFHSAGYSVSQNLPIWNGWGCT
                     LIPKPEPGVIVEMLEKFHPTFLTGVPTIYTALLGNQKFRTMDLSFVKGYFGGAAPLPE
                     NTLKQLKDLHGAIIYDVYGATENTAFATTTPWGGKVKIGTVGLPLPNTDMKIVDVDKG
                     VTELPIGEAGEICIKGPQVMSGYYKKPEETANSLRDGWFYTGDIGYFDDEGYLTISDR
                     KKDVIVASGFNIFPKEIDEILFEHPKISEACCIGIPDEYRGETVKAYIVVKAGETLTK
                     QEVTAFCKERMTAYKVPTEVEFIDELPKSAIGKIMRRELRELDRKKREKES"
     gene            complement(13911..14717)
                     /locus_tag="PLA58_03665"
     CDS             complement(13911..14717)
                     /locus_tag="PLA58_03665"
                     /inference="COORDINATES: protein motif:HMM:NF004794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOR61924.1"
                     /translation="MENESSLKVEKTGHVACLILNRPKQRNTMTIEFFQEIRRLFKAF
                     DEDGDVRVVVIRAEGKSFTAGLDLVAAQSLLGDGSAVYREELRRKIIDLQASMTAIEN
                     CRKPVIAAIHGHCIGGGVDLTSACDIRLASKDAVFSIRETRIGIIADIGTLQRVPYII
                     GQGWFRELALTGRDWTAEESLRMGYITRLCENPQTLFEEARTLAEEIANLPPLAIQGI
                     KEVVNYSRDHGIQQGLEYVAQRNAALVPNDDMMEAVVAFLEKRAPKFHGR"
     gene            15240..15584
                     /locus_tag="PLA58_03670"
     CDS             15240..15584
                     /locus_tag="PLA58_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61925.1"
                     /translation="MEDYIHHNIGEEVSFIAGSYQIVEEGCLTHDGREFLYLMGIATV
                     DNACCGNTGCRFLQVPGYIHSWKTKTNPQGKPVSEVEPITDEQNQAAIRTFLEACYPY
                     AQINFSGKSHIA"
     gene            15789..16469
                     /locus_tag="PLA58_03675"
     CDS             15789..16469
                     /locus_tag="PLA58_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR61926.1"
                     /translation="MKARILAVARRLFGEYGFHGTTTRMIAKEVGIDISTLHYHWGDK
                     NDLYEAVVMDVNNDLGRELLKVEKVVHGSSLEERLNVSIDQMMDYLFDHPEISNLVLL
                     RYFSKTRNEIDVDFKVPEFSGDIARAMGLAKDAKNPSPRAMMKVMSIMNGMHSFVSGE
                     NFFRPMLNLDRKNYIRAVKETLKFILIPAFVLQEAKTKKGAVPGPLPPKINKTPKQNA
                     RPVKQNNP"
     gene            complement(16615..17442)
                     /locus_tag="PLA58_03680"
     CDS             complement(16615..17442)
                     /locus_tag="PLA58_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR61927.1"
                     /translation="MLQCTIGCSHNQCAFCGMYKDKKYRVRRLREILDDIRMAKDYYG
                     DVEKVFLCDGDAIAIETETLLQILQALYATFPSLRHVGSYVGPQSTLTKSPDELRVLR
                     SAGLTKAYIGVETGDDELLKKVHKGVGRAEMLEAGRRLVEAGINLSAMVLLGLAGPGE
                     PSRRHALATAQICDEMKPQYLAALTVTPVPGTVLYRQVQKGEFQLLDPFETLAEMKMI
                     FENITLDPLKFVGTHASNYLPVTGTLQKDKQKMIALVDSVLTGRDGRLLRPDHLRGL"
     gene            complement(17516..18517)
                     /locus_tag="PLA58_03685"
     CDS             complement(17516..18517)
                     /locus_tag="PLA58_03685"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOR61928.1"
                     /translation="MIIQCKQCRTKFRFDDAKIAQEGIWVRCNRCRHVFFQEPPKKIH
                     STGAAPDGGEPPRSAPAAEKTGGRLAFEPAGPPAGKSPPDEDLVSFLQDVMETEKTAD
                     DEAKQGLPFADRESGDAPADAETFGEFSCGDAPRDALTFPRRRKSRAWKFALWTLLVV
                     AVVPALFYFAFFPALGERLLKAGGQGWENVLSMVGLSRPAGNLPVTSLVRLQDVRQRV
                     VENYILGPVRIVEGKAVNQAEFPISRVQVKAEILDAYAVVLNEQMSYAGNVLNDDELA
                     VMTEEEMKIRLSLPEGQNNANDRIEPNGRISFMVVFTGDPPGMMKTVVKIVGAERLL"
     gene            complement(18514..19068)
                     /gene="hpt"
                     /locus_tag="PLA58_03690"
     CDS             complement(18514..19068)
                     /gene="hpt"
                     /locus_tag="PLA58_03690"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOR61929.1"
                     /translation="MADVLKTVFLTPSEISGRVFEMAGEISRDYTGREILVIGVLKGA
                     FVFMADLIRALSVPCRTDFIRAASYGAGAESSGKIVITKDVELPVEGQDVLIVEDIVD
                     SGLTLRHIMDTLKSRNPRSIKVCAFLDKRKRRKISFEADYVGFSMDDGFIVGYGLDFN
                     EQYRFLPAVYVLDPAPAREKEENG"
     gene            complement(19140..19952)
                     /locus_tag="PLA58_03695"
     CDS             complement(19140..19952)
                     /locus_tag="PLA58_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61930.1"
                     /translation="MEDPIKVLCQPDAGKSCGACCGLYNYVDSSRESLVRRLSARTKR
                     YREMVKSPDDLPRYAAATFAAEDFRKRYEGIYCCEYLGFLDAEEKKVGCLVHPLQNDG
                     ADMRTVSFYGRDVCAGHLCPSHHFIPRSQQEILLKIIEDWYLYGLCLTDIDLVVAYFR
                     LIADRIGEELRPEAFDRQALKDAALEYFRWKTTWPFRSAEANRLGKYYFDGSQYMISH
                     IDYEKFGREISPLNAILLSLSSEFQTAEDLARAEKMIWSNIETAASLYRERV"
     gene            complement(20005..20865)
                     /gene="purU"
                     /locus_tag="PLA58_03700"
     CDS             complement(20005..20865)
                     /gene="purU"
                     /locus_tag="PLA58_03700"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOR61931.1"
                     /translation="MTEKSNAILLLSCPDRKGLVANLSNFVFQHNGNIVDADQYTSKR
                     GKIFFMRMEWELDGFALGRSEIPVAFKPLADQCGMKWTLHFTDDVPRMAIFVSRHLHC
                     LHDLILRRHLGELGGRVEAVISNHPDARDLVEQFGLRFFHFPITPNQKREQEDRQMAL
                     LRELNIDLVVMARYMQILTGGFIGQYVNKIINIHHSFLPAFSGRNPYQQAFDRGVKII
                     GATAHYATEQLDDGPIIAQDVVKISHRDTIDDIQTKSKDLERIVLARALRLHLDHKII
                     VYGAKTVVFE"
     gene            20982..21380
                     /locus_tag="PLA58_03705"
     CDS             20982..21380
                     /locus_tag="PLA58_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61932.1"
                     /translation="MKRIETLHLPNGLVLTINDLSRRIAADTVKVELSFQMKVNVEPS
                     FFASPDDHRQITGIFGDELTYEHKMERTFVAEAEESATRAELLETFKNNSLGYLSKPD
                     FAKRMALSLLSDIRRNPFKYRKNPFPGQAE"
     gene            21418..23889
                     /locus_tag="PLA58_03710"
     CDS             21418..23889
                     /locus_tag="PLA58_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOR61933.1"
                     /translation="MRPNIFDELLTGAEKPSRYIGAEVNAVRKENAGVRFLLAFPDTY
                     EVGMSHLGLQILYAVLNALPGAAAERCFAPWPDRERQLRERRLPLTSLETQSPIADFD
                     VIGFSLQYELSYTNILTMLDLGGIPLASADRRDAHPLILAGGPCAFNPAPLAAFIDAF
                     VIGEGEEAVVEIADMLAGSKKQKSGRAAMLEALANLDGVYVPAVHGPDKTIFKRKVIN
                     INRQRHPAAPVVPLMQTVHDRIVLEIARGCTRGCRFCQAGMIWRPYRERDAGLIMDMA
                     DRALAATGHDEISLLALSAGDYSCIEPLMQTLMRKHAAGRVALALPSLRVETLSRTLI
                     EEIKRIRKTSFTLAPEAGTDKMRRIINKGNTAEDLLTSVDNVFAAGWKAVKLYFMIGL
                     PLEEESDLDGIVDLSYQALRAARRRGQVTVSLSTFVPKPHTPFQWESQLSIDETFSRQ
                     NFIRRRMNNRSLAVRWHDARMSLLEGLFSRGDEKIGTLLLRAWRKGCRFDGWSEIFRF
                     DLWQEALAETEIPTGEYLRERSANEPLPWDNIDCGVSRDFLLQERQKALAGDATPDCR
                     YEACQDCGVCDFKDVQNIFSDQNLPAPTVEIRPPAGTVPEKVYRLSFAKTGRARFLSH
                     LELAAALTRALRRSSLSLCYSAGYHPHPKISFATATSVGMESRQEYLDVTALAYPQNL
                     SVLKGEINAALPDGMAITDLQALSFAARDLARSLWGFVYELILPADTNEEKLGVILQS
                     IENFLAAESFPITRASKGKTVTRDIRPFVEALSLNVQEKTVTLTARHAQTGSVRPVDI
                     LRHVLGFSEEEMQRIGVVKMKTMLA"
BASE COUNT         5296 a         7228 c         6119 g         5436 t
ORIGIN      
        1 tgcctttttt ctccttgtca aacatttcga caaccttgat gaccccggtc atcacatcgc
       61 ccggatgaat ggggagcaga tactcatact cttcttcccc gtgaagcagc cggaccaggt
      121 tgatgccgac ctcctgcagc atgggcatca atcctccgcc gccccacagc aaaaaacacg
      181 tttgaaacgt cgaaggggga acaatatcct tataccccgc cgcctttgcc gccccggaat
      241 cggcataaat ctgtttcacc tggctcttgc tctccttctg cgaaacggcc atagcgaact
      301 cggcgatttt tcccttttcc acctcgaaga tgtaaggggg aaacaccatc cctacttttg
      361 atttgtcggc catgctcacc tcccattatg atggtttatc atgatcggga ccttctcttc
      421 acgagaacag tcccgtcttc ccgaactcat tgatttcctc ctcgctgtaa cccagttcga
      481 ggaagacttc ccggttatgc gatcccgcct tgacgcccgt ttttccgtac accgggggag
      541 ttgcggaaaa cttgaacgga ctggccggtt gtcgtacggt gcttccatcc ggcagcgtga
      601 tgtccgtcgt caattgccgt tcccggacca attcgctgtc cagcgcttcc gaaagcgaaa
      661 gaacgggttc cacacacgca tccgttttgt cgaaaatttc cttccattcc ccgcgcgttt
      721 tactcttgat gatctttctt acttcctctt tgacgccggc gcagtccggc ggcgccacgc
      781 cgccggcaat ccaatcggga cgtccgatgg cgttgcagaa ggcggcaaag aattgcggct
      841 ccagcccgcc gaaactcagg taatggccgt ccgccgtttt ataaaaatca tagagggatc
      901 cgccgttgag gataaaccct tctcttgtcg gctcttcccc cgatacgaga taggcggcgg
      961 cagccagggc gttgaaggcg aaagccccgt cggtcatgga aatatcgaga tactgtcctt
     1021 ttccgctcct gctcctgctg aacaccgccg ccagaatggc gatgacggca ttattggatc
     1081 cggaagccac gtcggcaatc tgcaggggca tcggcacggg cccctcgtat ttgcgtcccg
     1141 cgtaacccat cagcccggag agggacaaat aattaatatc atggcccgcc cggtctttca
     1201 tcggccccgt ctggccgtag cccgtcagag agcaatagat cagggacgga taccgggggg
     1261 caagatcatc aaaacccaat ccaagcttcg ccatgattcc cggacggaat tgctcaatga
     1321 ggacgtcgta gcgctccagc aggcgatgga caacctgcag cccccgggca tccttcagat
     1381 tcagcgtaat ggatcgcttt ccgcggccaa gatacgccga ggcggcggtc agatcggtct
     1441 tcccgataaa cggcggcaca atggccgcca gatcaggccg gcttccggag gtaaccttta
     1501 aaacatccgc ccccaggtcg gaaagaatca tggtcgcata cggcccgggc aaaagcgtcg
     1561 taaagtcgag aacctttaaa ccttccagtg gtccggccat acgctataac cccataaact
     1621 tggcaataat ggttttcatg atttcattgg tcccggcaaa aatccggctc acccggctgt
     1681 cgcgccaggc ccgtgaaatc ccgtattcat cgcagtatcc gtacccccca tgcagctgaa
     1741 gacatctgtc cgccacctgg tgggccatgt ccgtgatcca gtatttggcc atcgacgtct
     1801 cgatgacaac ctgccgcccc tccatgtgat ccacaatcag cttgtccaca aaggtccgcc
     1861 cgatcttgat ctccgtggcg cattccacca gcgtgaactg attgacctga aacttggaga
     1921 tgggttttcc aaaaaccgtc cgttcccgac aatactggat ggtggtctcc agcatgtatt
     1981 cggcggccgc gattccggag atggcgacga ccagacgctc ctgctgaagc ttcatcatga
     2041 gtttcaggaa tccggttcct ttttcgccca gacggttctc tttgggaatt ctgcagtccg
     2101 tgaaatacag ctcggaggta tcctggctgt gccagccggc ttttttgatc tttttgccct
     2161 tctcaaagcc gggcgtgccg gcttccacaa caaaaagatc caccgctttg tgggggtccg
     2221 tacaggccgg atcttttgcc gccacaacac acagatcaca gttgatcccg ttgctgataa
     2281 aggttttctg cccgttcaag acaatatcat tcccgtcctc cacggcggtc gtcttgatcg
     2341 cggcaaggtc gctgccggta ttgggctccg tcatggccac cgccgtgata atgtcgccgg
     2401 tgacgcagcc gggaagatac ttttgcttct ggtcttcaga accgaaggaa ataatgtaag
     2461 gaacaatgat atcggtgtgc aggggcgccg caagtcccgc aaaattggaa tgcgacagtt
     2521 cttcgataac aatcaccgaa tacagaaaat ccgctcccgc gccgccgtat tccaccggaa
     2581 cggacatgcc cagaaacccg tgctccccca ttttcttcca ggcgctcttg ggaacgatgc
     2641 cggcctcttc ccattcctcg acatggggaa gcagttcatt ggcgacaaac ttccggaaag
     2701 tatcccggaa cattcggtgt tcatccgtat attgaataat atccattcat ccgtctcctc
     2761 atcaaaatgc attgtccgga cgcttccgac ctccgtgcaa aaaccgatcc gatcggagga
     2821 ggccgcggag gcggagaaag cggctccacc acctccgcgg cgcgagcgtc atccgcgtcc
     2881 tacagaatta ctttccatac ttacctacaa tggaaatcgc gcacacgttc tggtgaggga
     2941 aaccgcccag gttgtgtgtc aaaccgtatc gcggattttc gagctgacgc ttgtccgccc
     3001 gcccctgaag ctgcaggtac atttcataaa tcatccgcaa tcccgaggct ccgatcgggt
     3061 gtccgaaaca cttgagaccg ccgtcgatct gggccggaat tttgccgtcc cggtcataca
     3121 taccgtctaa ggcatctttc caggcatggc ctcgctccga gagatggaga tcctcatagg
     3181 ttacgagttc cgtgatggag aaacagtcat gacactcgaa catgctgatc tcttcgcgcg
     3241 ggttcgtgat tccggcttcc ttataagtgg ctgccgcgca tttgtccgtg gtcacgaaat
     3301 tcgctccgtc ccattcattg aagccgcctt cataaccgtt gctgaccgcc agctggacgg
     3361 ccttgaccgt gaccaggtcc ttcttctttc tgcccagttt ctgggcgatc tccggcgtcg
     3421 taacgatggc acaggccgag ccgtcgctga caccgcaaca gtcaaaaagc cccagaggcc
     3481 aggcgatgat cggggccgcc aggacctgat cgatcgtcac cgctttccgc aggtgagcct
     3541 tgggattcag ggcgccgttg gcgtggcttt ttaccgaaac acgggcaatg gcctgtttga
     3601 tgtcgtcatt tttgatcttg aacttggcga cgtaggcgga agcgagctgg gcaaaaagcc
     3661 ccggagcggt tgcattgggg aagaactgcc acatgagggt tcccgccgtc gaaccccagg
     3721 cagggagtcc tccgtacccc gtatccttca acttctcaac gccaagggcc agacaaatat
     3781 cataagcgcc cgacgcgacg ccgtaaaccg cgccgcgaaa tgcttccgta cccgtagcac
     3841 aatagttttc catcctggaa atgggaatgt tgggcagacg aagagccacg cccatcgacg
     3901 gagccccctt ccccacattg acctcatcca tacagacgcc gtaccaggcc gcctgaatat
     3961 ctttcttttc aatgcctgca tcggcgatcg cttcctgaaa ggcctcaacc atcagcccct
     4021 cggctcccac atcccatcgc tcaccgaacc ggctgcaccc cataccaatg atcgcgactt
     4081 tatctctaat tcctgaagcc atagcttcac ctccctctta attaaatttg aacagctttc
     4141 cgatcaactg agatttcatc atatcgccgc cgacctttaa tttcccggcg ctgaaggcct
     4201 tcatcccgtc aagctttccg gtaatcaggt caacaaaatc cgtatcagcc atgccgatgg
     4261 tggttgtcgg attgtctccc tttccggttg tgacgctgca cgttccatcc ttgacggcga
     4321 caaaccaatc cccgcctgcc ggaccggata tattgaactg gaaggtcacg tcttttccgg
     4381 ctgcggccgc cgcgttgaaa acggagggca ttttcttgaa gacccccgcg acgtccagcc
     4441 cgcttgcgcc gctttcaggc gccggtgcgc cggcggcagg aggcgtcaga aaatccataa
     4501 tggccatgtt gagttccgtg tagggtttcg ccccttccag gctgttgatc ttatcccagt
     4561 tgtcacgaat catttccggc gtcggcacat gcgtctcgtc ccccagcatc acgccggggc
     4621 cggtcagaat cgccgcccgg ctgaagaagc cggcccccgc attgaagatg ttgccggaat
     4681 ccttgcagtc ttcagaacac agatacagaa cgatgccggc gacgtaagcc ggattcattt
     4741 tctcgagcat ctccggcggc atcatatcct cggttaaccg ggatgcggcg atgggggcga
     4801 tggtgttgac cttgatgttg tatttcgcgc cctccagctt cagcgtgttc atgaagccga
     4861 caagacccag cttggctgcg gaataattcg tctggccgaa attgccgtaa aggccggccg
     4921 cggaagtggt catgatgatg cggccgtaac cgttctgccg catggcctcg aaagccggcc
     4981 tcgtcacgtt ataggcgccg ttgaggtgga cggcgagaac ggcccgccag ttttccggct
     5041 ccatcttcag gatgccttta tccctgagaa tgccggcgtt gttgatgagg atatcgactt
     5101 tgccgaaagc gtccagcgcc gtcttcacga tcttccgacc gccttcgggc gtggcgacgt
     5161 tgtcgtaatt ggcgacggct tcgccgccca tggccttgat gtcggcaacc accagatcgg
     5221 cgggaccggc ggctcctgct cccgatccgt cgcgcgcgcc tccgaaatca tttacgacaa
     5281 ccttggctcc gcggcgaccc agctccaggg catacgcccg gcccaaaccg ccgccggcgc
     5341 ccgtgacaat ggccacccgt ccctgaaaat cgatttccgg tatccagtcc gtctggggaa
     5401 cggccttcca gaagtattgc gagaatcccc ggaccgtatc cacgacccgt ctgcggaaag
     5461 acatcttgac gggcatgccg actttgatgt cgttcaactc actgtcggtg aaatccatca
     5521 tgagccggcc gccgccctca aactgcacca tgccgtaaat cgccggtgga tccaccgata
     5581 cagccagcag atctcccgta aacgagcgga ctctggcctt gatattggca aattcgtaat
     5641 cgtcctgtgt tttcaggtga ccgcaggccg gattcacaca cagatccgcc ggtggatact
     5701 gagccgttcc gcaatcccgg cattttccgc cgacaaaccc gaggatcatt ttccggttcc
     5761 gccacagagc cgtcatggcc gtctgcaccg gcgcttccga ccgaattccc atctccggat
     5821 tgatcagatc ccggaatttc aggaacttca tgtagttgtc cgtggtcttc ttattggcca
     5881 gcgatccttt gagtcccgcg cgctcggcca gttttccgat attctccgta accttgaaat
     5941 aaagagcgtc acagccctgt ccgaaaccgg ccagaagaat gccgtctccc ggctttgccg
     6001 tttcgagcgc tccgaccagc atcaggagag catgcgcaac gcctgtctct ccggtgactt
     6061 cgtgcatcgt atcggccagt ttttccggag cagcccccag ctttttcccg atcgcccggt
     6121 gttccgcctt gaacaaacag gggaaaacca gtttttgcac gtcattcatc gaaatattca
     6181 atttcttgaa caggccgttc accgcttcgg gaatgatttt ggaatagcct tcttccctga
     6241 gccacctctc ttcccaggtg tagtcgaact cggatttgga tccccggtaa tgatccacga
     6301 aatcgtaggt gagggagtag gaacccagga actccgccac aacgtcttca tccccaacca
     6361 ggaccgacgc cgcgccgtcg ccgaaccaca tttcgtaaaa gccggccgga cgggtctgcc
     6421 gtttgtcgga cgcggtcacc agaatggatt ttcggctccc cgatttgacg acatccagag
     6481 cggtcaggag tccggtcgtg ccggccctct ggcaagacgt aaaatccgcc gtattgatgt
     6541 cgctccgcag attcagagcc gtggccagaa tgccggcatt ctggcgatcc aggaaaggca
     6601 ggctcgtgga gcacaggtat cccgcatcga tttttgtttt gtcttttcca atcaaacaat
     6661 ccatggacgc ggcgacggcc atcgtcaggc tgtcctcatc gtaattgcac atggaacgtt
     6721 cgccctgggc cacactgata agcgccggag cgaaccagcc catcgattga taaatagcca
     6781 ttctgtccaa tcgcaggcgg ggaatatagg ctccgtatga cgtaattccg atcatcgttt
     6841 tacctccttt gttcacaaga cattgcgttc aaatttttaa accgacgctt cagaaaatct
     6901 ccgctctaaa acttgaagat tttctccaaa agctgggatt tcatgatgtc tccctcgatc
     6961 ttcagttttc ccgaggtata ggccttcatg gcattgacct tgccgttcat cagatcgaga
     7021 aaatcccccg cctgaatctt cagcgtgcag gtcggactgg catgcaggcc ggacgctatg
     7081 gtgcaagcgc cgtttttgat gtcgacggac caatccccgc cgccgtcgcc cgagatgcag
     7141 tactggaaca caaccgccgc tcctcccgcc gcgcctgcct gaaaggcccc gggcattttt
     7201 tcaaagacat ccttcaccgc ggcgaaaccg ccgccgcttt gcgccggctc tttcttcccc
     7261 tccaacgccg agagaaggtc ctgcaggacc agatcgttca attgtccgta ttccttggct
     7321 cccttgagat ccatgatctt cgcccagttg acggagatgt cctcgacgga cggaaccttc
     7381 ttgccgctcc cgatgacgac gccgggactg gtcatcatgg cggcgcgatt gtaaaatccg
     7441 gcgccgcagt tgtaaattct gccggtctca ttgcattctt cagagcccag atagagcacg
     7501 atgggcgcaa catacgcggg attcattttg gccagcagat cgggcggcag aatatcctcc
     7561 gtgagacgtg acgcggcaat gggcgccacc gtgttcactt taatgttgta cttgatgcct
     7621 tcaagcttca gggtgttcat gaagccgaca agacccagtt tggccgagga gtagttactc
     7681 tggccgaaat tgccgtaaag ccccgcggcg gacgtcgtca tgatgatccg tccgtagcca
     7741 tggtcccgca tgacggcgaa agcgggacgc gtcacatgga aggccccgtt cagatgaaca
     7801 tccagaaccg cctgccagtt ttccggctcc attttaagaa tgcccttatc ccgcagaatg
     7861 ccggcattgt tgaccagaat gtccactttc ccaaaagcat ccacggccgt cttgacgata
     7921 ttttctcccc cgttcgcggt cgcaacatta tcgtaattcg caaccgcctc tccccccagg
     7981 gctttgatct catctacgac tttgtccgcc ggaccggccg ctccggggcc tgcgccgtcc
     8041 ctggcgccgc cgaaatcatt gatcactact tttgcgcccc gccgggcaaa ctcaagagcg
     8101 tacacacgcc ccagtccgcc gcccgctccg gtgacaacgg ccacccgacc atcaaaacgg
     8161 atctccttac ttgctcccat catccttctc cttttcgtga aatgtctctt gaaagcaatt
     8221 tcattgaatc cttggacaaa acatctacgc cggaaagtta aaacagcttg ttcactctcg
     8281 ccagcgtctc ctcggcctct ttcaccattc gcgccaacag ttccttgcag gtgggcacat
     8341 cctggataag tccaatcgac tgccccacca tcatgggagc gacctcgacg tcgccgtctt
     8401 cccatgcctc tttcattcgc tcaccggcga tcaggggata cagagcctcg aaccccccgc
     8461 ctttctcctc aatttcacag atattcttaa ccacctggtt cttgagcgcc ctgccctgca
     8521 gatgaagggt tttacagatc agcgtggtct catattcctg gcgtttcaca atttcctctt
     8581 tgatattatt gtggaccaca caatctttgg tggcaatgaa acgactggcc atcataaccc
     8641 cctgcgctcc cagcgtgagc gctgccgcaa gggacttgcc gttggcgatg ccgcccaccg
     8701 tcaccacggg gattttcacg gattcgacaa tacggggggt caacaccatg gttgtgacat
     8761 catcatccag cggatgtccg ccctcctcga tgccggcggc gtaaatgccg tcatagccgg
     8821 ccttctccgc gtgcagggca tgacgcaccg accccacctt atgaaaaagt ttaacccctg
     8881 cttttttcag caggtcgatg tactcgggac ccaccgcctt gtccaccgga gcgccggaca
     8941 cttcaatgcc cgccactttt tcttccgcgc aaacccgcag gtacatctgg tgatgccccg
     9001 ccgtgatgcg cacggaagga agaatggtga ttccgaccat gaacggcttg tcggtaagtt
     9061 tacgggtctg acggatagcg ttgcggaagt cctcttccgt ttcataattt cccgcggtaa
     9121 gatttcccat cccccccgcg ttggaaaccg ccgcggcgag cgtcggttta caaagccaca
     9181 tcatcgcccc acaaataatg gggtacttga tcccaaacat ctcggtgatt gccgttttca
     9241 tttgaatcct cctttagtat ttcaacctgc catgcgtgat tcatccggtc acccaagcgg
     9301 cgctccggat ttttgtcttg cttcccgtca cgcgcaggga tgagacgaaa acactgcgtt
     9361 ccccggcagg aaatcaaccg tttcgccgag ggatattcct tccatctttc caaagtattt
     9421 cggaaactcc gtcgccagcc agaattccga cgcgcaaacc ttgccgtaat agaaagccgc
     9481 ctccgcgtcc cgcgccagaa cttccgtcag ggcttcccct ttcgcgccgg ccgtaagcgc
     9541 ggaaagcttc ggacgcgcca gcgtcagaca ccacagatgc atccaggcga gcatcaggtc
     9601 gaaaaaggcc cttcgcaggg gatcaacgtt taaaagcagc gtgtggtatc gcccctcatc
     9661 catatgcttt ttcatggtat ctaaaatgga atccatccgt tggagtccct caaggagcat
     9721 attgcggtaa acgtcgtcca cgatgccgga tgcgttttcg atcgtctggt atatccttga
     9781 tttcaacacc ttgtaattct tccgatccgc attcaacagg atcttcctca tgaccaggtc
     9841 cagggactga atgccgttgg ttccttcgac gatgctcaga accttgcagt ccaccgcaag
     9901 ctgctccacg ggataatccg agcaatagcc atagcccccg tgaacctgga tggcttcggc
     9961 ggtgatctgc catgcatttt ccgaattgcc cgccttgatc aggggaataa gaatatcgac
    10021 gatagaccga gcttcccggc ttgcttctcc ctcgagcaga ttggacagat cgatctgcat
    10081 agccgcataa taggagagca tccgctgtgc ctcgacgaga gctttcattc ccatcagcat
    10141 tctcgtaacg tcgggatgtt caatgatggg cactttggga gcggccgcct cgaacatttt
    10201 gctgaagtga acgccctgca gccgggtgcg cgcgtaatcc acggcatact gcaaggccgc
    10261 ggaggaaccg cccagcgcca tgatgccgac ctcaagcctt gtttcattga gcatctgaaa
    10321 catgatattc attccgcggc cccgttcgcc cagcagatac cccgtacagt tttcattatc
    10381 cccgaaggac atggcgcagg tcgccgatcc gtgggcgccc atcttgtgtt ccacgccggt
    10441 gcagaacaca tcgttccttt ctcccaggga tccatcctcg cggaccagat acttgggaac
    10501 gatgaaaatg gaaattcccc tggttcccgg cggatcccct tcaatgcggg ccaggaccgg
    10561 atagatgatc tggcgggtaa tgtcctggtc tccgttggta atgaaacact tctgcccggt
    10621 aattcgatac gttccatcgg gctgcaagat ggcttttgtt ttcaaagccc ccacgtcgga
    10681 gcccgcctcc ggttccgtca ggcacatggt tccgccccat tcaccggaaa ggagtttttg
    10741 cacgtagagg gacttctgct ccggagtccc gaaactgcgg attaaatgag cggccccgtg
    10801 ccccagcatg aaaaagagca tcaacgctcc gccggccccg cagaatatct cccacgtaca
    10861 cgactggatg gcgaggggca tccccgcccc tccgatttcc tggtcgtcgg caatgctcag
    10921 gaatcccgca tcacaaagcg ccctggccgc gtcggaatac cccgacggca atcgaaccga
    10981 tctggtttgc ggatcatagc ggcacccttc cctgtgcccg gtcacattcg ccggaaagac
    11041 ccattgggcc gcaatctttt cggccagtcg gatcgtctcc tcataaacgt cgccgtcaaa
    11101 attttcaaaa cgccggaagc ggttcatttt gtccacttcc agaaattcga acagggtgaa
    11161 gcgcaaatca cgatgatcca cgagatattt ctgcattaca gattctcctc cacgggaaac
    11221 gtcggccaca gggttcgttt ggttgcggtc ctctgcgtat ggcctttaca cagccgaccc
    11281 gtcggtcacg ctttataaaa tttctggacc aatttaaaaa actcgcctga aaaaaccatg
    11341 atggtctgat tccggttttc aaggttcacg gccgcctcca gcgagggagc atggaggttt
    11401 tcattcagaa cgcgcttcgt cagtttcaag cccccctcgc tcttgcccac catggacctt
    11461 gcgtagaaaa gcgctgcttc cagaagatcc tcccgcggga ccagcttgtt gacaaggcct
    11521 attctttccg cttcctcccc gccgatcttc ctgccggtga aaagaatatc ggaggcgtgc
    11581 gcgagcccta ccagccgggg cagcaaaaag gaacagccca gttccccgcc ggaaagcccg
    11641 atattaatga aagaggctac aaaaaaggct tccttgcagg ccacccggat atcgctggcc
    11701 agcgtcatgg aaaatccgcc tccggcggcc ggcccgttca cggccgatat aataggctgg
    11761 ggaatccttc tcatgcccag aatcagagcg gcgtaacgct cctgcacaaa tttcaggaat
    11821 ctctccggat ccgcaaacgc ttcggactcc ttgtagatca cggcgtcatt gagatcggct
    11881 cccgaagaat accccttgcc ggcgcccgtg aggatgagga cccggatgga atcctccccg
    11941 gacaagacct caaacagtct ttcgaagtcg cccagcatat ccagattaat ggcgttgagc
    12001 tgcccgggac ggttcattgt caccacgccg atgcgcggct ctttttcttc aaagagcaat
    12061 gtcttgaatt ccggcatcaa cgtactcatg attctttctc tctctttttc ctgtccaact
    12121 ccctcaattc gcggcgcatg atcttgccga tcgcgctctt gggaagttca tcaatgaatt
    12181 ccacttccgt aggaacctta taagccgtca ttctctcttt gcagaaggct gtcacctcct
    12241 gtttcgtcag cgtctctccg gccttgacca caatgtaggc ttttacggtt tccccccgat
    12301 actcgtccgg gatcccgatg cagcaggcct cgctgatctt gggatgctca aagagaattt
    12361 catcgatctc cttggggaaa atattgaagc cgctcgcaac gatgacgtct tttttgcggt
    12421 ctgaaatggt caggtatccc tcatcgtcga agtatccgat gtctccggtg taaaaccatc
    12481 catcccgaag ggaattggct gtttcttccg gttttttata gtacccggac atcacctggg
    12541 gacctttgat gcagatttca cccgcttcac cgatgggaag ttccgttacg cctttatcaa
    12601 catccacgat tttcatatcg gtgttgggaa gcggaagtcc caccgtcccg atttttacct
    12661 tcccgcccca gggagtggtg gtggcaaacg ccgtattctc ggtagcgccg tagacatcgt
    12721 agatgatggc cccgtggaga tctttcagct gcttcagggt gttttccggc aggggcgccg
    12781 ccccgccgaa atatcccttg acaaaagaaa gatccatcgt ccggaatttt tgattgccca
    12841 gaagagccgt ataaatcgtc ggcacgcccg tgaggaaggt cggatgaaac ttttccagca
    12901 tttcgacgat cacgcccggc tccggcttcg ggatcagcgt acagccccag ccgttccaga
    12961 tgggaagatt ctggctgacg gaatacccgg cggaatgaaa cacgggatag atccccgtga
    13021 gattctggta ttggcccttc aggtcgggaa accaaacgga aaactgctgg acgaccgatg
    13081 agatgttggc atgggtcagc atggcgccct tgctgacgcc ggtcgttccc ccggtataga
    13141 tcagcgcggc cagcgaatcc cacttggcct tgttttccac cggtgtgtcc ggataccggg
    13201 cgatcaaatc catgaattcg taaacatccg gctgcggttc aaccttccgg aacattgctt
    13261 ttttaacata ggggaaaagc tgcttcttgg gaaagggcag gtagtcgctg atgtggcagg
    13321 tgatgatttt tttgatcttg gtttcgcttt tgatcttgag ggcccggggg agcagcagat
    13381 ccagcgtgac caggacggtg gcatccgaat cgtcgagttg gtgggttaat tcccgctccg
    13441 tataaagcgg attgttcatg gcggtcgcgg cccccgcccg ataggtggca tggtcggcaa
    13501 tgatcatctg cgggatgttg ggcaggagca tcgccacctt gtccccttcc cgcacgccga
    13561 tgtcgatcaa tgcccttgtg aaccggttga ccagtttctc gagctgccgg taggtaattt
    13621 ttttacccat gtagatcatg gcaactcttt caggataggt ggcggccgtt ctcgtcaggg
    13681 cctccgccat ggtgatcttt tccacagtaa tttcggcagg aacacctgaa gcataggatt
    13741 tgtgccatat tctttcaaaa gacatattga tcctccttct cctttgtttt gtttaccagt
    13801 tcttcagctc attcttttcg gcccgttacc gggttgtgcc aacactcctc cgtcatgtat
    13861 cttctgtttc tacgcccaac ggcgcagaag aaacctatgg tttgggattt ctatcgtcca
    13921 tgaaactttg gcgcccgctt ctccagaaag gcgacaaccg cttccatcat atcgtcgttg
    13981 gggaccaggg cggcgttgcg ctgagccaca tattccaggc cctgctgaat accgtggtca
    14041 cgactgtaat tgacgacttc cttgatgccc tgaatggcga gaggaggcaa attggcgatc
    14101 tcttcggcca gtgtccgggc ttcctcgaaa agggtttggg gattctcaca gagccgcgta
    14161 atgtatccca ttctcaagga ttcctccgcc gtccagtccc tgccggttaa cgccagttcc
    14221 cgaaaccagc cctgtccgat gatgtaggga acgcgctgaa gggttccgat atccgcaatg
    14281 atgccgattc gggtttcccg aatggaaaag acggcgtctt tcgatgccag ccggatatcg
    14341 caggcgctgg taagatcgac gcctccgccg atacagtgtc cgtgaatcgc ggcaatcacg
    14401 ggctttctgc aattttcgat ggcggtcatg ctcgcctgaa gatcgatgat ttttctgcga
    14461 agctcttccc ggtacacggc cgatccgtct cccagcagag actgggccgc cacaagatcc
    14521 aatccggcgg taaaactttt tccctccgcc cggatgacaa ccacccgcac gtcgccgtct
    14581 tcgtcaaacg ccttgaaaag ccggcgaatc tcctgaaaga attcgatggt catcgtattg
    14641 cgctgcttcg gccggttcag aatcaggcag gccacgtgac cggttttttc aactttgaga
    14701 gacgattcgt tttccatgct gccttctcct tttactccac aacaagattc ttccgtgtcc
    14761 ttccccgttc aacctcgcca cacgattctc caatcaattc ttccatctat taattccatc
    14821 aattgatggt taacctattt ggaaaagtct gctttgtcaa gttatttttt taaaaaaagc
    14881 cgtcgaattc catttccctg tatttactgt caattctcta cagccaccaa ccggcatcct
    14941 gtcgggtgtt tcgcagaaat ctcctcttcg ccgggaaaaa cgatccggat aaaccatctt
    15001 gactttttct atcatttaac atatcggcac cctcaaccga tcgcaattgc cgaccgtccg
    15061 ggccgtcgag gttgtttccg gagaaaccca gcgccatgaa tcgtccaggg cttgatcgga
    15121 ggcgcggtaa aacaggacgt ccggaaaaga ccggcgcgga gggctctgcg cgaaagattt
    15181 cgccctgcgg ccctgaataa agaacagcgc cggcgaaaat aatcgggagg aagcgcacca
    15241 tggaagacta catccatcac aacatcggcg aagaagtttc ctttattgca ggatcttacc
    15301 agatcgttga agaaggatgt ctgacgcacg acggaagaga gtttctttat ctgatgggaa
    15361 ttgcgaccgt tgacaatgcg tgctgtggaa acaccggctg tcggtttctt caggtcccgg
    15421 gttacattca ttcatggaag acaaaaacaa atccccaggg gaagccggtc agcgaagtgg
    15481 agccgattac cgatgaacaa aatcaggcag ccatcaggac ttttctggag gcttgctacc
    15541 cctatgcgca gatcaatttt tccggaaaat cacacatcgc gtgagcctga cggcggcggt
    15601 tactcaaggg atgccggatg cggcggaagc gatccggttc tttttcaacc ctgccccgaa
    15661 aagatccctg aggtcccgtc aaaattaaaa accttgacgg ctgcacggta ttgtcgtagg
    15721 tattcaatca aatgatggag taaaaaatga aatcacccct tgaaaaggcc cgtaagaatc
    15781 ctgaatccat gaaggcaaga attcttgccg ttgcccgccg tctttttggt gaatacggct
    15841 tccacggaac caccacccgg atgatcgcca aagaagtggg aatcgatatc tccacccttc
    15901 attaccactg gggcgacaaa aacgacctgt atgaagccgt cgtcatggac gtgaacaacg
    15961 atctgggaag agagcttctc aaggtggaaa aagtcgtgca cggcagctcc ctggaggaac
    16021 gactgaacgt ctccatcgac caaatgatgg attatttgtt tgaccatccg gagatatcga
    16081 accttgttct ccttcgctat ttcagcaaaa cccgcaacga aatcgatgtt gatttcaagg
    16141 tgccggaatt cagcggcgac atcgctcgcg ccatgggtct cgcgaaagac gcaaaaaatc
    16201 cgtcccccag agccatgatg aaggtcatga gcatcatgaa tggaatgcac agctttgtct
    16261 ccggagaaaa ttttttccgt cccatgttga accttgatcg aaaaaattac atccgtgcgg
    16321 tcaaggaaac cctgaaattc atcctgatcc ccgcttttgt ccttcaggaa gcaaaaacaa
    16381 aaaagggcgc agttccgggt ccgcttccgc caaagattaa caaaaccccc aaacaaaacg
    16441 cacggccggt aaagcagaac aacccctgac ccgatcggcg gccgccggcc tttcccatgc
    16501 gcaggcggta gactgtcatc tgtcgcccga tctcccaaac atccgccgcg gccggaaacg
    16561 atccggcccg ccggtgggaa accttttcat gccgcagatc accgaaacag cgactcaaag
    16621 tccccgcaga tgatccggtc gcagcagccg tccatcgcgg ccggtgagaa ccgaatccac
    16681 cagggcaatc attttttgtt tgtctttttg aagcgtgccg gtgaccggca gataattgga
    16741 cgcatgcgtg ccgacaaatt tcagcgggtc cagggtaatg ttttcaaaaa tcatcttcat
    16801 ctccgccagg gtttcaaaag gatccagaag ctgaaattcg cctttttgca cctgccggta
    16861 gagcacggtc cccggaacgg gcgtcaccgt cagggccgca aggtactggg gtttcatttc
    16921 atcgcaaatt tgcgcggtgg caagcgcatg gcgtcgtgaa ggctctcccg gaccggcaag
    16981 tcccagaaga accatcgccg ataaattgat cccggcttcc accagcctgc gcccggcttc
    17041 cagcatctcg gcgcgtccga ctcccttatg aacctttttc agaagctcgt cgtcgccggt
    17101 ttcgacgccg atatacgctt tggtcaggcc ggcggaccgc agcacccgca attcgtccgg
    17161 agattttgtc agcgtgctct gcggcccgac ataactgccg acatgccgca gggaaggaaa
    17221 agtcgcataa agtgcctgga gaatttgcag cagggtttcc gtttcgatgg caatggcgtc
    17281 gccgtcgcac aggaacacct tttccacgtc gccgtagtaa tctttggcca tgcggatgtc
    17341 gtccaggatc tcccgcaggc gccgcacgcg gtatttcttg tctttgtaca tgccgcagaa
    17401 ggcgcattga ttatgggagc aaccaatggt gcattgaagc aagtaactcg cggcctcgct
    17461 gaagggccgg aatatatttc cttcgtatct caaagcatcc tacccggcgg agccgctaaa
    17521 gcaaacgctc cgcgccgacg atcttcacca cggtcttcat catgcccggc ggatccccgg
    17581 tgaaaaccac cataaaagaa attctcccgt tgggctctat ccgatcattc gcattatttt
    17641 gcccctccgg cagcgaaagc cggattttca tttcttcttc ggtcatgacg gccagctcgt
    17701 catcattcag cacatttccc gcgtaactca tctgttcgtt cagaacgacc gcataggcat
    17761 ccaggatttc cgcttttacc tgcacgcggg agatgggaaa ctccgcctga ttgaccgcct
    17821 tgccttctac aatgcggaca gggcccagaa tgtaattctc gacgacccgc tgacggacat
    17881 cctgaagcct gaccagcgag gttacgggca agtttcccgc cggcctagag aggccgacca
    17941 tggaaaggac gttctcccat ccctgcccgc ccgctttcaa aagacgttcg ccaagcgcgg
    18001 ggaaaaaagc aaaataaaac aacgccggaa caacggcaac gacgagaagc gtccacagcg
    18061 cgaacttcca tgccctgctt ttcctgcgcc ggggaaaagt caacgcatcc cggggcgcat
    18121 ccccgcatga aaattccccg aaggtttcag catcagcagg ggcatccccg ctttcccggt
    18181 ccgcaaatgg aagaccttgt ttggcttcat cgtccgccgt tttttccgtt tccatcacgt
    18241 cctggagaaa gctgaccaaa tcctcatccg ggggactttt gccggcgggc ggacctgccg
    18301 gctcaaaggc cagccgtccc cctgtttttt cggcagcggg agcggagcga ggaggttcgc
    18361 ctccatccgg cgcggctccg gtcgagtgaa tttttttggg aggctcctga aaaaaaacgt
    18421 gccggcagcg gttgcagcga acccagatgc cctcttgtgc gatctttgcg tcgtcaaaac
    18481 ggaatttggt gcggcactgt ttacactgga tgatcatccg ttttcctctt tttcgcgcgc
    18541 cggcgcgggg tccagcacat acacggccgg caaaaagcga tactgctcat tgaaatccag
    18601 gccgtatccg acgataaaac cgtcatccat cgaaaacccg acgtaatccg cctcaaaaga
    18661 aatctttcgt cttttgcgtt tatccagaaa agcgcagact ttgatggaac ggggatttct
    18721 gcttttcaat gtatccataa tgtgacgcag cgtcaggccg ctgtccacaa tgtcctcgac
    18781 aatcaggacg tcctgaccct caaccggcaa ttcaacatcc ttggtgatca cgattttacc
    18841 ggagctttcg gccccggcgc cgtagcttgc cgccctgatg aaatccgtcc ggcagggaac
    18901 ggaaagggcg cggatcaaat ccgccataaa aacaaaggct cccttgagaa ctccgatcac
    18961 cagaatttcc cggcctgtgt aatcccggga aatttcaccg gccatctcga aaacgcggcc
    19021 ggatatttca gacggcgtca gaaacactgt ttttaaaaca tcggccatgt ccccatgctc
    19081 cccgatgatg aaattgcccg ttgatattac tcaagattcc tcttgcctgt caattaaaat
    19141 caaacccgct cgcgataaag cgacgccgcc gtttcgatgt tggaccaaat cattttttcc
    19201 gctcgggcaa gatcctccgc cgtttgaaac tcagaagaca aactgagcaa aatggcattg
    19261 agcggtgaaa tctccctgcc gaatttttca taatcaatgt ggctgatcat atattgtgat
    19321 ccgtcaaaat aatatttacc cagacgattg gcctccgcag aacggaaagg ccaggtcgtc
    19381 ttccagcgga agtattccag cgccgcatcc ttcaacgcct gccggtcaaa ggcctccggc
    19441 cggagctcct ctccgatccg gtcggcaatc agacggaaat acgcgaccac caggtcgata
    19501 tccgtcaggc acaggccgta cagataccag tcctcgatga ttttcaataa aatttcctgc
    19561 tgggaccggg ggatgaaatg gtggctgggg cacagatgac cggcgcaaac gtcccgcccg
    19621 taaaaggaaa cggtgcgcat atcggcgccg tcgttctgca ggggatgcac gaggcacccg
    19681 actttctttt cttcggcatc cagaaagccg agatattcac agcagtaaat cccctcatag
    19741 cgcttccgga agtcttccgc ggcaaacgtg gcggcagcat agcggggcag atcatccggc
    19801 gacttcacca tttcgcggta gcgttttgtg cgggcagaca acctcctgac cagcgactct
    19861 cgcgagctgt ccacatagtt gtaaagaccg cagcaggcgc cgcaggactt gccggcatcg
    19921 ggctgacaca atacctttat gggatcttcc atggacatga aaacctttga gatgaatccg
    19981 catcatgaaa taagaaagtg atgcttattc aaacaccacc gtcttggcgc catacacaat
    20041 aattttatgg tccagatgca ggcgcagcgc ccgcgccaga acaattctct ccaaatcctt
    20101 gctcttggtc tgaatgtcat cgatggtgtc gcgatggctg atttttacaa catcctgagc
    20161 gataatggga ccgtcgtcca actgctcggt ggcataatgc gccgtcgccc cgataatttt
    20221 cacgccgcgg tcaaaagcct gctggtaagg gtttcttccc gaaaaagcag gcaaaaacga
    20281 atggtggata ttgatgattt tgttgacata ctgcccgata aacccgccgg tcagaatctg
    20341 catgtaccgc gccataacca ccagatctat gttcagttcc cgcaaaagcg ccatctgccg
    20401 gtcttcctgt tctcgctttt gattcggggt aatgggaaaa tgaaagaagc ggagcccaaa
    20461 ctgttcgacc agatcgcgcg catcgggatg attgctgatg accgcttcca cccggccgcc
    20521 cagctcgccc agatgcctcc gcaggatcag gtcgtgcaga caatgcaggt gtcgggaaac
    20581 gaaaatggcc atgcggggca catcatccgt aaaatgcaaa gtccatttca tgccgcattg
    20641 atcggccagc ggcttgaacg ccacgggaat ctccgagcgg ccgagcgcaa agccgtccag
    20701 ctcccattcc atgcgcatga aaaagatttt tcccctcttt gaggtatatt gatccgcatc
    20761 gacaatattg ccgttatgct gaaaaacaaa attggataga ttcgccacca accccttgcg
    20821 atcggggcag gataacaata aaattgcgtt ggacttctcc gtcatgcttc cttccttgga
    20881 ttcgcaagtc acgggatcgc ttataaaagg attaccattg caggtcaaga ataaaaatgg
    20941 taagaacccc ccactttgca aaaacgcggg aaagaaaaac catgaagcga atcgagaccc
    21001 ttcatctgcc caacggcctt gttctgacga tcaacgatct gtcgcgccgc atcgccgcgg
    21061 acacggtgaa ggtcgagctc tcctttcaga tgaaggtcaa cgtcgagccg tccttttttg
    21121 cttcgcccga tgaccaccgg cagatcaccg gcatcttcgg agacgagctt acctatgagc
    21181 acaaaatgga aagaacgttc gttgccgaag ccgaggagtc ggccacccgg gcggaacttc
    21241 tggaaacctt taaaaacaac tctctgggct atctgtcaaa gccggatttt gcaaaacgaa
    21301 tggctttgtc cctgctttcg gacatccggc gcaacccctt caagtacagg aaaaatcctt
    21361 ttcccgggca agcagaatag acccggaaca acgcaaattc caaacggacc ataaaacatg
    21421 aggcccaaca tttttgatga gctgctgacc ggcgcggaaa agcccagtcg ttatatcggc
    21481 gcggaagtca acgccgtccg gaaggaaaac gccggcgtgc gatttctgct ggcctttccc
    21541 gacacctatg aagtgggcat gtcgcatctg ggcctgcaaa ttctgtatgc cgtcttaaac
    21601 gcgctccccg gcgcggccgc cgaaaggtgc tttgccccct ggccggaccg ggaacggcag
    21661 ctgcgggagc gccgccttcc cctgacctcc ctggagaccc agagccccat cgccgatttt
    21721 gatgtgatcg gtttttcgct gcagtacgaa ctgtcctata ccaatatcct gacaatgctg
    21781 gacctgggtg gcatcccgct tgcgtcagcc gatcggcgcg acgcccatcc cctcattctc
    21841 gcgggcggcc cctgcgcgtt caatcccgcg ccccttgccg ccttcatcga cgcctttgtc
    21901 atcggggaag gcgaagaagc ggtggtggaa atcgccgaca tgctggccgg gagcaaaaaa
    21961 caaaaatccg gaagggccgc catgctcgaa gcgctggcga accttgacgg ggtttatgtt
    22021 ccggccgtcc acggcccgga taaaaccatt ttcaagcgga aggtcatcaa tatcaaccgg
    22081 cagcgccatc ccgccgcgcc ggtggtgccg ctgatgcaaa ccgtccacga ccgcatcgtt
    22141 ctggaaatcg cccggggctg tacgcggggc tgccggtttt gtcaggcggg catgatctgg
    22201 cgcccttacc gggagagaga cgccgggctg atcatggaca tggccgaccg cgcgcttgcg
    22261 gcaaccggtc atgatgaaat atctcttttg gcgctcagcg ccggagacta cagctgcatt
    22321 gagccgctca tgcaaaccct gatgcggaag cacgccgccg gacgcgtggc cctggccctg
    22381 ccttcgctgc gggtcgagac gctgagccgg acgctgattg aagaaatcaa gcgcatccgc
    22441 aaaaccagct tcaccctcgc gccggaggcc ggaacggata aaatgcgccg gatcatcaac
    22501 aaaggcaaca cggcggaaga tttgctgaca tccgtggaca acgtctttgc cgcgggatgg
    22561 aaagccgtca agctgtattt catgatcggc ctgccgctcg aagaagaaag cgatctggac
    22621 ggcatcgtcg atctgtccta tcaggcgctg cgcgctgccc gacgccgggg ccaggtcacc
    22681 gtcagccttt caacgtttgt cccgaaaccg cacacgccct ttcagtggga atcccaattg
    22741 tcgatcgacg aaaccttctc tcgccagaat ttcatccgcc ggcggatgaa caaccgcagt
    22801 ctggccgtca ggtggcacga cgcccggatg agcctgctgg aaggcctgtt ttcccggggt
    22861 gatgaaaaaa tcggaacgct gcttttgcgc gcctggcgga aaggctgccg ctttgacggc
    22921 tggagtgaga ttttccgctt tgatctgtgg caggaagccc ttgcggaaac ggaaatcccg
    22981 accggcgaat acctgcggga acgctccgca aacgagcctc tgccctggga caacatcgac
    23041 tgcggtgtga gccgggattt tctgctgcag gaaaggcaaa aagcccttgc gggagacgcc
    23101 acgccggatt gccgctatga ggcctgccag gattgcggcg tctgcgattt caaagatgtt
    23161 caaaatattt tttcagacca aaacttgccg gctccgacag ttgagatcag gccgccggcc
    23221 gggaccgttc ctgaaaaagt ctatcgcctg agcttcgcca aaaccggccg ggcccgcttc
    23281 ctttcgcatc tggagctggc cgcggcgctt acccgcgcgc tcaggcgcag ctccctgtcc
    23341 ctgtgctact cggcaggcta ccacccgcat cccaaaatat ccttcgccac ggccacctca
    23401 gtgggcatgg aaagccgtca ggaatacctg gacgtcaccg ccctggcgta tccgcaaaat
    23461 ttgtcggtct tgaaaggcga aatcaacgcg gccctgcccg acggcatggc catcacggac
    23521 cttcaggcgc tttcctttgc cgcccgtgac ctggcccggt ctctgtgggg cttcgtttat
    23581 gaactgattc ttcctgcgga cacaaatgag gaaaaactcg gcgtcattct tcaaagcatc
    23641 gaaaattttc tggcggccga atcgtttccc atcaccagag cgtccaaagg caaaaccgtc
    23701 acccgggaca tccggccttt tgtcgaagcc ctgtccttaa acgttcaaga aaaaaccgta
    23761 acgctgaccg cgcgccacgc gcagaccggc tccgtccgcc ccgtggatat tttgaggcat
    23821 gttttgggct tttccgaaga ggagatgcag cggatcggtg tcgtcaaaat gaaaacgatg
    23881 cttgcctgaa gaccgccccg cggtctgaag atccgaaagg agaaaacggg atgaaaaatt
    23941 tcatcaggat tagtgtttgg ctgttgttgt tcgtcagcct tgcaggatgc cacggcgcag
    24001 cccccgaagc acgcgacgac cgattcaccg tcattgctca ggacatgccc taccccacgg
    24061 atcaatatat ccggatcgg
//
LOCUS       DAOSRM010000022        22978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2414_length_22978_cov_113.329102, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000022 DAOSRM010000000
VERSION     DAOSRM010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 22978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22978
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_22978_cov_113.329102"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..355)
                     /locus_tag="PLA58_03715"
     tRNA            complement(268..355)
                     /locus_tag="PLA58_03715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Leu,seq:taa)
     gene            897..2222
                     /locus_tag="PLA58_03720"
     CDS             897..2222
                     /locus_tag="PLA58_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOR61934.1"
                     /translation="MHDIDLIISGGKALLLDEANTCLDNAGIAVHDSSIIDIGRKEEI
                     LQRYAAKKIIDAEDSLVLPGFVNTHTHAAMTCFRGIADDLELMEWLNDYMFPAEAKNV
                     NPHLAYWGSLLACAEMIKSGTTTFCDMYIFEEETARAAKEAGVRCLVGEVLFDFPSPS
                     CKTTEEGLAYTRRLAEKWYNDPLVKIIVEPHSLYTCSRPLLQKAKIIADEFGLMLGIH
                     LLENQQEHDQLLEKHGKSAVAFLDDIGCLDEKLLAFHCVYLTLDDIHRFAKHGCKVSH
                     NPASNMKLGNGIPPITDMLEAGITVGLGTDGCASNNTLDMIKEMSTAAKLHKVAMLDP
                     TVMDAKTAMRMATINGAKALGMGHRVGSLETGKKADIILIGLNKPHLTPMYNAWSHMV
                     YAAGGADVDTVIINGKVVMENRRLLTIAEEDVMNEVRKIAVGVKKSMNM"
     gene            2238..3815
                     /locus_tag="PLA58_03725"
     CDS             2238..3815
                     /locus_tag="PLA58_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOR61935.1"
                     /translation="MFRKIRRHTWIGILPFLAAGLLCMPSVSPAAVREVTLFPDSARV
                     EETVKIDLPSAGANKNQVILVLPSQADPESLTVSPSSAPRVKIDDIRIKSIARIDENR
                     IAQLRAQLKKAQNEKKELTARLKALEIQIQFWQAQTKAKTKTVSEADTLAAAIGRSSR
                     KIFSEKNAVEADMENIDKQIKEIQENLDRTAGKSEKAWEATVTLSGAAPRDALLIYSY
                     TLGGCGWQPLYRMEALPSSGNVLFSWDAEIWQSTGEEWKTAQIYLATLQPVRTIAPPE
                     LPPWMIKPKTPVIYKSSYREKAAQARLSMEETNDVMAAGAPAPVETVRTTYSVWSLGK
                     KTVAPGDRQRLKIKEETWPAQFLFLARPSLSPQAYLQAKIQLPGPAEIPSGEATFVID
                     GAIIGKRPFALAGKEADIYFGNSPFVTVSSLTVADQSGGAKFFSNKQTRRWQWRLEAK
                     NTGNTPVKLRIEEPIPQPRDERIKLTFKQQPEPSEKSEAAFVWLVDLPALQKKIIETG
                     VELEAPQDMALDFGWRR"
     gene            3838..7800
                     /gene="hrpA"
                     /locus_tag="PLA58_03730"
     CDS             3838..7800
                     /gene="hrpA"
                     /locus_tag="PLA58_03730"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOR61936.1"
                     /translation="MLPSDHPHKRLIRLVERQARDIMIADYHRLLQSLQTANASPGGR
                     PAPGADAASRLQNRMDASRRRVQKRRQSVPALNFPPELPICAKRQTIVDAIRNHQVVI
                     ITGETGSGKTTQIPKMCLEAGLGLRGVIGLTQPRRIAAVSIAARIASELGQDCGEAVA
                     YKIRFEEKTSAEPLIRVMTDGILLAETPGDRNLLAYDAIIVDEAHERSLNIDFILGYL
                     RSLLPRRKDLKVIITSATIDTAKFAQAFDGAPVVEVSGRMYPVEVRYRPLDPAKEEKG
                     ETTYVDEAVACVEDLQRQGRFEDVLIFLPTEQDIREACERLAKRCSGLVLPLFARLSS
                     GQQKLVFQPSSHQKIIVATNIAETSLTIPGIKYVIDSGLARMLEYNPRSQTTGLPVKP
                     VSQSSAEQRKGRCGRVQNGICIRLYSAEDFADRPLYTAPEILRSNLAGVVLRMLSLNL
                     GLISSFPFIDRPHPKSIRDAIEILRDLGAIRREENAEDSEQYQLTPIGRTMAQFPLDP
                     RVSRMILEAKKHDCIHEIAVIAAALSIQDPRERPYDKIDQATAAHARFAHPESDFLTY
                     LQIWNRYSDHFDQMKSQSQWRKFCRDHFLSYRRMKEWRDVYSQIMDIIGGKATSPSRR
                     KPARMEIDQTIADKIHRSILSGYLSNIAQKKEKNFYTAAKSREVMLYPGSGLFNRGGP
                     WIVAAEISLTSRVFARNAANIQSEWLEELGGDHCRCQHWAAHWEKSRGQVVASEKVVL
                     FGLTIVEHRSVAYERINPEEAKKIFIREALVTGETGRKLPFVEHNLALWEKVKTMEDK
                     LRKRGIAADEEAIAGLYEQKLPLLSDIRSLQKWIKDRGGDDFLRFREEELIVGGPDPE
                     ELKQYPDSIRLGNAVLSCRYRFEPGRAADGVTVRVPLGLVSRTASENIEKHLPSLRQE
                     KVLHWLKSLPKNIRQKLPPPIQVAGQFGKRTGDKDRELPAALGRFLFEEYHLTVPSGF
                     WAPEKLPPHLQIRFSVVDDHGGEIRSSRDIGELRQELTEQVRRTSLESCRRQWEKENL
                     TGWDFGRLPEDIELSGKHGVTGCAYPALQAEGEAVHLRIFADSRQAAAHHAAGVSALY
                     GIHFTDRLKQLRKNLSLGGELKEQAALLGSVRQVERSLLERVKKDLFQRSWRSPEEFM
                     KHAEDVQPRILSYGQEVIAAAAPVLRSFAETHEGLQKLIFKNRSHPAVAKFLQAAEVE
                     MRSLVPEDFMLRYDFDRLRHLPRYLKALLLRAGRGSLNLASTESKLREAAIYTAKLQE
                     IREAAGADLSEEKRNKTEELRWMIEEYKVSLFAQELKTPYPVSPKRLNQLIEEIGNIL
                     "
     gene            complement(7929..9113)
                     /locus_tag="PLA58_03735"
     CDS             complement(7929..9113)
                     /locus_tag="PLA58_03735"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOR61937.1"
                     /translation="MSISKKIKASMSQSSLIRKMFEEGNLLKSRHGHDKVFDFSLGNP
                     NVDHPREFKKELVRVARQEILLKHGYTPNAGYPETRRAVARKISRTTGLKMHPDHIVM
                     SCGAGGALNVILKSILDPGDEVIILKPFFVEYPFYIENYGGVVKTAATRPDFSLDPDA
                     IGRAITRRTKAIILNSPNNPTGRVYSKTNIAALVRLLKEKEKKYNRAIYLLSDEAYAE
                     ILYDGIKLPSVLTAYDNSIVAYSYSKTLSLPGERIGYIAVNPKVHEADDLIRALILCT
                     RIMGFVNAPALMQRIVANLQHITVDVKIYQKKRDLLCRGLARAGYKFIKPQGAFYLFV
                     KSPVPDDVAFAQRLVQKNILVVPGSGFGTPGYFRIAFCVDDATIRNSMKGFAAAIREC
                     RS"
     gene            complement(9157..9819)
                     /locus_tag="PLA58_03740"
     CDS             complement(9157..9819)
                     /locus_tag="PLA58_03740"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HOR61938.1"
                     /translation="MLFQQKKALVLSDFDGTISRVDVGDGVLSRFASESREAIDSAYI
                     RGGMGSREAYGKIAPLVRVDAGRLRAYVLKCSAIDPYFSRFYRLAAKKGVDVKIVSDG
                     LDFYIRAILDKHRLGEIEFYSNTVAFGGEDTLTFDFPQANALCGRCGTCKSKILHDHR
                     LMYEKIIYVGDGHSDICPSQYADLIFAKGELLKKCRRESAVPYRAFHDFREVSEYLKK
                     NV"
     gene            complement(9848..10159)
                     /locus_tag="PLA58_03745"
     CDS             complement(9848..10159)
                     /locus_tag="PLA58_03745"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOR61939.1"
                     /translation="MPNEKRNILTGGLILITLGVLIFISKTTSYSFGQTWPILLIVIG
                     ITTLFYSIRAVEGWFVATVGVIFLIIELYGFDLYKYSQYILPVILIVLGVFVLLKRRK
                     R"
     gene            complement(10223..10987)
                     /locus_tag="PLA58_03750"
     CDS             complement(10223..10987)
                     /locus_tag="PLA58_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOR61940.1"
                     /translation="MLGSLPLPLLVVIVLVVMFLASAIKILNEYERAVLFRLGRLRDI
                     KGPGLIVIIPGIDKIVRIDMRTITMDVPPQDVITKDNVSIKVNAVVYFRVLDANAAVV
                     NVENYLFATSQLAQTTLRSVCGQVELDEILTEREKINLNIQEILDRSTDPWGIKVSLV
                     EVKHIDLPEEMKRAMAKQAEAERERRAKVINAEGEFQAAQKLIEAAALMGTQPMSLQL
                     RYLQTLNQIAAENNSTTVFPIPIDLFKPFLNLAGKS"
     gene            complement(11013..12323)
                     /locus_tag="PLA58_03755"
     CDS             complement(11013..12323)
                     /locus_tag="PLA58_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOR61941.1"
                     /translation="MLKKTSFFFVILLWIAAAGVVFAQESKPLFHVIELNGVITSPSA
                     KYVAQSLEEATAAKAEGLIILLDTPGGLDTAMRDIAKSILNAPLPVIVYVYPSGARAA
                     SAGVIIAQAAHVAAMAPGTNIGAAHPVAIGLGGSGEMDKTMSQKVENDAAAYARSIAK
                     TRGRSEEWVEKAVRKSESITADEALKLNVIDIVAVDIGKLLAAIDKRTVTVAGAKKKI
                     STAHAVLDTKKMGTRQGILAAISNPNIAYLLLLIGLAGLYFEFSTPGAILPGVIGGIS
                     LLLAFFALSTLPVSYTGILLILFSVILFIAEIKIMSHGMLTVGGVISLALGSFLLFDS
                     PEPALRISLQVLLPAVLVASGFFIVIIGLAVKAQMRKHYSGTEAMIDGRGEAATDIHG
                     DGKVFFQGEYWTATSDREIPKGAPVRIIKVEGLKLVVEEIKEGK"
     gene            complement(12340..13068)
                     /locus_tag="PLA58_03760"
     CDS             complement(12340..13068)
                     /locus_tag="PLA58_03760"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOR61942.1"
                     /translation="MTKSFPSPSSDAIRNDLLSLVKSVRGQVLDADRLYVAVDCKRPS
                     PAPKIKSDGRDRAEAFHALSLEAAKCRQCPLAQTRKNVVFGAGSLSAQVVFVGEAPGA
                     DEDEQGLPFVGKAGQLLTNIITAMGLTRKEVYICNILKCRPPGNRTPLPEEIRLCEAF
                     LKRQLSLIAPQIICALGSVAAKALLRTETPISALRGRFQLYEGIKLMPTYHPAYLLRN
                     PSAKKQVWEDVQLIMKEIGPGGRS"
     gene            complement(13065..14279)
                     /gene="coaBC"
                     /locus_tag="PLA58_03765"
     CDS             complement(13065..14279)
                     /gene="coaBC"
                     /locus_tag="PLA58_03765"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOR61943.1"
                     /translation="MLKNKKIVLGITGGIAAYKSAELTRAFVKAGAEVKVIMTRSATE
                     FITPLTLRTLSRNPVYTGLFEDAAPYDMAHIALAEWADAFVIAPATGNVIGKIASGIA
                     DDLLTTTIMAENKPTLICPAMNDKMLANPFVQENLRKLAGIGYRVMQSGVGELACNAT
                     GAGRLPEIPEILDELEALFTVQDFAGEKVLVTAGPTQEPLDPVRYITNFSSGKMGYAL
                     AKAAHQRGAKVTLISGPTKIAPPKVETVIFVRTAQEMHRAVMDACSKASVIIKAAAVA
                     DYRPRITAAEKIKKDRGPAAVELVRNPDIIAAIGAKKKKGLLVGFAMETQDLLANARQ
                     KMIRKKMDMIVANNLRDEGAGFETDTNIITILDRQGHSIALEKMTKQEAAHRILDHIR
                     SLQTKKGPAGKK"
     gene            14453..15775
                     /locus_tag="PLA58_03770"
     CDS             14453..15775
                     /locus_tag="PLA58_03770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR61944.1"
                     /translation="MKKTGIIAAVCAIILLAGASGWLYFAGWLDREKPAIQLDSDISA
                     IGRKKDIGITFADERSGLSGLKVELVQDNKPHLLAEETFPSRSTRQKVLRLSVEPLAL
                     QLNNGPAVVRITARDASFFKNETVVSEQTRIDTLPPQVYVMSPVNYINQGGAGFIPYR
                     TSKPAALTGVFVDDRFYPGYNIVIGGKPTSVAFFALGTGASAATKIVIFARDNAGNEA
                     RTSMPFTIKPKKFRADKVNLSETFLQKIMPEFQASIPQLQGKTPVEVFGYVNTTLREE
                     NAKTIQSVCRKSINKKLWEGAFHRMRDAAPMALFGDQRTYLVDGKPFGNSVHLGVDLA
                     STAHAPIEAAANGIIVFAGALGIYGNAVIIDHGLGLFSLYGHLSAIETTVGKSVKKEE
                     KIGVSGLSGLAGGDHLHFSILVGGEFVNPQEWWDPHWIEDNVMKKMTL"
     gene            complement(16039..16905)
                     /gene="rapZ"
                     /locus_tag="PLA58_03775"
     CDS             complement(16039..16905)
                     /gene="rapZ"
                     /locus_tag="PLA58_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOR61945.1"
                     /translation="MKNLQVVIITGLSGSGKSTALRALEDIGFFCVDNLPVVLLPKFL
                     SITLESSPQIKQVALVMDLRQKSFLEKYQRIFERLKEKGYRIKILFLEAGEESLLRRF
                     SETRRSHPLSEKRSIMEGIQAETEKLATLRQMADIVIDTTTSNVHQLKDTVQKQFLPS
                     STRKKMMISVMSFGYRYGLPADADLAIDVRFLPNPFYVEKLKNMTGHHRDVEKYVLNN
                     EECKTFIVKASEMLNFIIPLYEKEGKVRLNIAFGCTGGKHRSVVMANQFSLFFQKQKF
                     IVHTTHRDINKS"
     gene            complement(16906..17448)
                     /locus_tag="PLA58_03780"
     CDS             complement(16906..17448)
                     /locus_tag="PLA58_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61946.1"
                     /translation="MQSHRLPAAASPLHENLLASRIRSILREKIHRCVTLHGVALACG
                     ERGILLTGPSGIGKTTAALAAVRKGYDWIADDLAVIKSIQGRLMISGHPGIQQYIHTQ
                     AAGIAAADRMLDGSRRRKKAELAFVIDVFRATREEISRPLETRILETNLPLVRVGIPR
                     TGYLRQNLLGRAIQKLNEVG"
     gene            complement(17768..18235)
                     /locus_tag="PLA58_03785"
     CDS             complement(17768..18235)
                     /locus_tag="PLA58_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOR61947.1"
                     /translation="MRLDQIFKMEFLNENLLSDTKEGVLAELINILIESGLKIDRARA
                     IDVLQQREKLGSTGIGDGVAIPHGKVSDLQDMVIAFGRSRRGIAFNAIDGKPVRLFFL
                     LLAPEHSTGQHLKALARISKMLKTPNFRKTLIDAKTKNDLYKAIVEQDASCPL"
     gene            complement(18349..18879)
                     /gene="raiA"
                     /locus_tag="PLA58_03790"
     CDS             complement(18349..18879)
                     /gene="raiA"
                     /locus_tag="PLA58_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOR61948.1"
                     /translation="MNISVTFRTGEGENWQKAYAEEKIARLKKYLDVPAEVHMILSTE
                     KFRNVVEINLTANGWNVNAKEEAKDMHLAVDSCIVKIEKQLKKQKEKNRQHKPKSIRH
                     PGGKSLEGPEEDDASSKVVETRKIILKPMSLEEAVLELESSKARFLIYRDTASEKVSL
                     VHRLDDGKFAHIETNS"
     gene            complement(19035..20507)
                     /gene="rpoN"
                     /locus_tag="PLA58_03795"
     CDS             complement(19035..20507)
                     /gene="rpoN"
                     /locus_tag="PLA58_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOR61949.1"
                     /translation="MAFELRQNLKLSQQLIMTPQLQQAIKLLILSRQELVDTINQEME
                     ENPLLEEAAPDEASAEEIRAEVEEDVQSIEKEESKIPEHTAELTGEGDGREEFDWNNY
                     LEDYGPVGVSYDRAEGDAPTLDNVLTESPSLTEHLMWQMKLSPFTEEEVRIGQQIVGN
                     LDANGYLCATVAEIARLENVGEDLVENVLKKVQEFDPAGVAARNLQECLLIQAGMFAG
                     ENPLVETIIREHLKELELKNYLQIARRLKVPLKEVEMAVLLISKMDPKPGAVYSEEKV
                     QSIIPDVYVVKSGDEYKIVLNDDGLPRLRISNFYREIMGGIGGQDSAEKENGRKYIRE
                     KVQSATWLIKSIQQRQKTIYKVAESIVKHQKDFFDKGIDYLKPLVLRNIADDVEMHES
                     TISRVVTNKYMHSHRGIFEMKYFFGSSIQRTSGENIASKSVKEEIRQIISREDPKKPY
                     SDCEIVKLLKARNIDIARRTVAKYREMTGILPSSKRKKYF"
     gene            complement(20535..21257)
                     /gene="lptB"
                     /locus_tag="PLA58_03800"
     CDS             complement(20535..21257)
                     /gene="lptB"
                     /locus_tag="PLA58_03800"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOR61950.1"
                     /translation="MSHLSARGLVKIYNKRKVVNQIDLAISPGEVVGLLGPNGAGKTT
                     TFYMIVGLVKPDGGEIFLDDEEISAYPMYKRARKGLNYLPQEPSIFRKLTVEENILAI
                     LETLEISEEDRRQRLKELLGELDLTSLAKNHAYSLSGGERRRVEITRALVTSPKYILL
                     DEPFAGIDPLAVADIQKIIEKLKNKGIGIIISDHNVRETLACCDRAYIINEGAILVQG
                     QPDVIARSELAKKFYFGEDFSL"
     gene            complement(21294..21848)
                     /gene="lptA"
                     /locus_tag="PLA58_03805"
     CDS             complement(21294..21848)
                     /gene="lptA"
                     /locus_tag="PLA58_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HOR61951.1"
                     /translation="MPIKKTGLIVLGFLFALNIQAVAQTGGGSKNPLRKNEPIQIVSD
                     KMEAFQASRMVVFSGNAVAEQGDVRIQADRLSIYYKDSGDSKEKKVDPGMEQAGDLEK
                     IVASGHVVITQKDVLATGNEAVYYQDTARFVMTGNPVLQQEKSVIKGCRVIIQVDENR
                     GRVEQCADENSGRVTAIIHPRKQK"
     gene            complement(21861..22430)
                     /gene="lptC"
                     /locus_tag="PLA58_03810"
     CDS             complement(21861..22430)
                     /gene="lptC"
                     /locus_tag="PLA58_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOR61952.1"
                     /translation="MIYKTMRFSKKAKIAAIALMILVLAGAVLYAFLRAPGGKKENLV
                     KILPDEADVRIADFFYTEVGKDDLRWEVKAKTAQYQKKQNLALFDQVQMKLVTQKGKT
                     FVMTGDKGEMRTDTKDVEIRGHVVVTADAGEKFSTDVLRYSDARKEIYTDAPVRMETS
                     RMKIQGVGLSILMDKGELALASGVKAKIY"
     gene            complement(22427..>22978)
                     /locus_tag="PLA58_03815"
     CDS             complement(22427..>22978)
                     /locus_tag="PLA58_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOR61953.1"
                     /translation="IRGKLEKIKMLILDVDGVMTDGRIIMDSDGREMKNFYVRDGHGL
                     VMLQRHGIQVAILTGRTSLVVDHRARDLKITEVYQGALNKKDVFARILEKNCLTPEQT
                     AYLGDDIVDIPVLRLVGFSAAVADAADEVKKQVDYVTVCRGGQGAVREICEMLLTAQG
                     FWPEIAGRYDFAAPESLYTEEGG"
BASE COUNT         5053 a         6653 c         6130 g         5142 t
ORIGIN      
        1 acgaggcaaa acgggacgtc tggcgggaag tattggacgg catagacgaa atcaaagccg
       61 gaggcctcga tccctataaa ctggccttca aggaatctgc cctgactgaa ccatatcata
      121 aagaacggca tcgagatcat ccactgatcc gctgttcgcc tctctaaacc acgccaaacc
      181 ttcctcaaag tggcaatctt ttgaatcatt gaacaaaaga aaaaggacct gtggaatgaa
      241 agaatgtcac aagcgcttga tttcatttgg tacctggggt cggaatcgaa ccgacacgaa
      301 gctaagcctc gagggatttt aagtcccttg cgtctaccag ttccgccacc caggccgatg
      361 gtgattttgc caagaaacct cgcggcatat attatttttg tctggatgtg tcaaggattt
      421 gctcttttgt ggccagccgc acggccctac ctgagggaca tcgggaaata gattcccgcc
      481 ttcgcgaatg acatcattag aagcaggata tcacaagcac ggattcgatg gattgccgtt
      541 tgcgtgggaa ataatggcag ggatggtctc ttgagagctt tgaaaagtgt catttcgagg
      601 agcgtgagcg acgagaaatc ttaaggatgg ccgtttgttc aagatttctc ccttcggtcg
      661 aaatgacaaa aatggccgtt aatgaacaac cccgccgcaa gcagcggggt atcaaaagct
      721 ttctattttg tatgggccat tcgccgcaag cggcggggaa tggaacccta agagattcaa
      781 agccctcctc ttttcccttc aggatgtctt cgcgtcgttc ccgcgaggga tttttgtttc
      841 ttgtggcccg tccgtgtttt atgataagca cccacttgtt ttgaaaggat tccttcatgc
      901 atgacatcga cctgatcatc tccggcggaa aagcgctcct gctcgacgag gcaaacacct
      961 gtctggacaa cgccggaatc gccgttcacg attcatccat cattgacatc ggccggaaag
     1021 aagagattct gcagcggtat gccgcaaaaa aaatcatcga cgccgaagac tccctggtgc
     1081 ttcccggttt cgtcaacacg cacacacacg ccgcgatgac ctgcttccgc ggaatcgcgg
     1141 acgatctgga gctgatggag tggcttaacg attatatgtt cccggcggag gcgaaaaacg
     1201 tcaatccgca tctggcttac tggggatcgc tcctggcatg cgcggaaatg atcaaatccg
     1261 gcacgacgac gttctgcgac atgtacatct ttgaagagga aacggcccgc gcggccaaag
     1321 aggcgggcgt ccgctgcctg gtcggcgagg tccttttcga ctttccctcg cccagttgca
     1381 aaaccaccga ggaaggcctc gcctacacgc ggcggcttgc cgaaaagtgg tacaatgatc
     1441 ccctggtcaa gattatcgtc gaaccgcatt cgctttacac ctgttcgcgc ccgctgctgc
     1501 agaaggccaa aatcatcgcc gacgaattcg gcctgatgct ggggattcat cttctggaaa
     1561 atcagcagga gcatgaccag cttctggaaa aacacggaaa aagcgcggtg gcttttttgg
     1621 acgacatcgg gtgcctcgac gaaaagctgc tggcgtttca ctgcgtctat ttaaccctcg
     1681 acgacatcca tcggttcgcg aagcacggct gcaaggtttc acacaatccc gccagcaaca
     1741 tgaagctggg caacggaatc ccccccatca cggacatgct ggaagccggc attaccgtgg
     1801 ggctgggaac ggacggctgc gccagcaaca acacgctcga tatgatcaag gaaatgtcca
     1861 cagcggccaa actccacaag gtcgccatgc tggatccgac cgtcatggac gccaaaaccg
     1921 cgatgcgcat ggccacgatc aacggcgcaa aagccctcgg catgggacac cgggtcggtt
     1981 ccctggaaac cgggaaaaaa gcggacatca ttctgatcgg actgaacaag ccccatttga
     2041 cgccgatgta caacgcctgg tcccacatgg tctatgcggc gggcggcgcc gatgtcgata
     2101 cggtcatcat caacggcaaa gtggtcatgg aaaaccgacg cctgctgacg attgcggaag
     2161 aggatgtcat gaatgaagtc cgcaaaatcg cggtgggcgt caaaaaaagc atgaacatgt
     2221 aaaggaaagg atgcgctatg tttcgcaaaa ttcgcaggca tacctggata gggatattgc
     2281 cgtttctggc tgccggcctg ttgtgtatgc cttctgtgtc cccggccgcg gtgagggaag
     2341 tgaccctttt ccccgattcg gcaagggttg aagaaaccgt caaaatcgat ctgccgtccg
     2401 cgggcgcaaa taaaaatcag gtgattctgg ttttgccgtc gcaggcggac ccggaatccc
     2461 tgacggtttc tccgtcttcg gccccgcgcg tcaagatcga cgatatccgg atcaagtcca
     2521 tcgcgcgcat tgatgaaaac agaatcgccc aactgcgcgc ccagttaaaa aaagcccaga
     2581 acgaaaaaaa ggagctcacg gcccggctca aagcgctgga gatccagatt cagttctggc
     2641 aggcgcagac caaggcaaaa accaaaacgg tgagcgaggc ggacacgctg gcggcggcca
     2701 tcggcagaag cagccgcaaa atattttccg aaaagaacgc cgtggaagcg gatatggaaa
     2761 atatcgacaa gcagatcaag gagattcagg aaaatctgga ccggaccgcc ggcaaaagcg
     2821 aaaaagcatg ggaagcgacg gtcacgctgt ccggggccgc tccccgcgac gcgctgctga
     2881 tctacagcta cacgctgggc ggctgcggct ggcagcccct ctaccgcatg gaggcgctgc
     2941 cctcttccgg caatgttttg ttttcctggg acgcggagat ctggcagtcc acgggagaag
     3001 aatggaaaac ggcccaaatt tatctggcca cgctgcagcc ggtccggacg attgcgccgc
     3061 cggagctgcc tccctggatg atcaagccga aaactcccgt gatctataaa tcatcctatc
     3121 gggaaaaagc cgcgcaggcc agactcagca tggaagagac caatgacgtg atggcggcgg
     3181 gggcgcccgc gcctgtcgaa acggtccgca caacctattc cgtctggtcg ctgggaaaaa
     3241 agacggtggc gcccggggac cgtcagcgtt tgaaaatcaa ggaagaaacc tggcctgcgc
     3301 agttcctttt cctggcgcgc cccagcctct cccctcaagc ctatctgcag gcgaagatcc
     3361 aactgccggg accggcggaa atcccttccg gggaagcgac gttcgtcatt gacggcgcga
     3421 tcatcggcaa gcgtcccttc gcgctcgcag gcaaggaagc ggacatttat ttcggcaaca
     3481 gccccttcgt cacggtcagc tctttgaccg tcgcggatca atccggcggc gcaaagttct
     3541 tttccaacaa acagacgcgc cggtggcaat ggcgtctgga agccaaaaac accggaaaca
     3601 caccggtcaa actgcgcatc gaagagccga tccctcagcc gcgcgatgaa agaatcaagc
     3661 tgacgttcaa acagcagccc gagccttcgg aaaaaagcga ggcagccttt gtctggctgg
     3721 tcgatctgcc cgcgttacag aaaaagatca ttgaaaccgg cgtcgaactg gaagcgcccc
     3781 aggacatggc tctcgatttc ggctggcggc gctgacggcg ggcactgacg gggaatcatg
     3841 ctgccttcag accatcccca taaacggctg attcgcctgg tggagcgcca agcgcgcgac
     3901 atcatgatcg ccgattacca tcgcctgctg caatcgctgc aaaccgcgaa tgcctctccg
     3961 ggcggacgcc ccgcccccgg agccgatgcc gcctcccggc tgcaaaaccg gatggacgcc
     4021 tcgcgccgcc gcgtacaaaa gcggcgacag tccgttcccg cgctgaattt tcctcccgaa
     4081 ctgcccatct gcgccaaacg ccagaccatt gtcgatgcca tccggaatca tcaggtcgtt
     4141 atcatcaccg gcgaaaccgg atcgggcaag acgacgcaga tccccaagat gtgcctggag
     4201 gcggggctgg gcctgcgggg tgtcatcggg ctgacgcagc cccgaaggat tgccgccgtc
     4261 agcatcgcgg cgcgcatcgc atccgagctc ggccaggact gcggtgaggc tgtggcgtat
     4321 aaaatccgct ttgaagaaaa aacatcggcg gagcccctga tccgggtcat gaccgacggc
     4381 atcctgctcg cggaaactcc cggggatcgg aatctgctgg cctacgatgc gatcatcgtc
     4441 gatgaagcgc acgaacgcag cctcaacatc gatttcattt tggggtactt gcgaagcctg
     4501 ctgcccagaa gaaaagacct gaaggtgatc atcacgtccg ccacgatcga caccgccaaa
     4561 ttcgcgcagg cattcgacgg ggcgccggtt gtcgaagtct cgggccggat gtatccggtg
     4621 gaggttcgct accggccgct ggacccggcc aaagaggaaa agggcgaaac gacctatgtc
     4681 gatgaagccg tggcctgtgt ggaggatttg cagcgccagg ggcgctttga ggatgttctg
     4741 attttcctgc ccacggagca ggatatccgc gaagcctgtg aacgcctcgc caaacgctgt
     4801 tcgggtcttg ttttgcccct gtttgcgagg ctttcctccg gacagcaaaa actggttttt
     4861 caaccctcct cccatcagaa aatcattgtc gcaaccaata tcgcggagac gtccctgacg
     4921 attcccggca tcaagtatgt cattgattcg gggctcgcac gcatgctgga atacaacccc
     4981 cgcagtcaga ccaccggcct gccggtcaaa cccgtttccc aaagcagcgc cgagcagaga
     5041 aaagggcgct gcggccgcgt tcagaacggg atctgcatcc gcctttattc cgcggaagat
     5101 tttgcggaca ggccgcttta tacggcgccg gaaattctgc gctccaacct ggccggcgtg
     5161 gtgctgcgca tgctgtcttt gaatctgggg ttgatctcct cttttccctt tatcgaccgg
     5221 ccgcatccga aaagcatccg cgacgcaatc gaaatcctcc gcgatctggg cgcgatccgc
     5281 cgcgaagaaa acgcggaaga cagcgagcag taccaactga cgccgatcgg ccggaccatg
     5341 gcgcagtttc cgctggatcc cagggtgtcg cggatgatcc tcgaggccaa aaagcacgac
     5401 tgcattcatg aaatcgccgt gatcgccgcg gcgctgagca tccaggaccc ccgcgaaagg
     5461 ccctatgaca agatcgatca ggcgaccgcc gcgcatgccc gatttgctca tccggaatcg
     5521 gacttcctca cgtatctgca gatctggaac cggtattcgg accatttcga tcagatgaaa
     5581 tcccagagcc agtggcgcaa attctgccgc gatcactttc tttcctaccg gagaatgaaa
     5641 gaatggcggg atgtttactc gcagatcatg gatattatcg gcggcaaagc gacgtcgcct
     5701 tcgcgcagaa agcccgcccg gatggaaatc gatcagacga ttgccgacaa aattcatcga
     5761 tcgattctga gcggctatct ttccaacatc gcgcagaaaa aagaaaagaa tttttatacg
     5821 gccgccaaaa gccgcgaggt gatgctttat ccgggttcgg gccttttcaa tcgcggcggc
     5881 ccctggattg tcgccgccga aatcagcctg acctccaggg tttttgcccg caacgcggcc
     5941 aatatccaaa gcgaatggct cgaagagctg ggcggggatc attgccgctg ccagcactgg
     6001 gcggcgcact gggaaaaaag ccgcggacag gtcgtcgctt ccgaaaaagt cgttttattc
     6061 ggattgacca ttgtggagca cagaagcgtc gcgtatgaac ggatcaaccc ggaggaagcc
     6121 aaaaaaatat tcatccgcga agcgctggtc acgggcgaaa cgggccgcaa acttcccttt
     6181 gtggagcaca acctggcgct gtgggaaaaa gtcaaaacga tggaagacaa gcttcgcaaa
     6241 cgcggcattg ccgccgatga agaagccatc gccggacttt atgaacaaaa actgccgctc
     6301 ctttccgaca tccggtccct gcagaaatgg atcaaagaca gaggcggcga tgattttctg
     6361 cgcttccggg aggaagagct gatcgtcggc ggccccgacc cggaggagtt gaagcaatat
     6421 ccggattcga tccggctggg caatgccgtt ttgtcctgcc gctaccggtt tgaaccgggc
     6481 cgcgcagccg acggcgtgac cgtccgcgtg ccgctggggc ttgtttccag aaccgcttcg
     6541 gaaaatatcg aaaagcatct tccgtctctg cggcaggaga aggttttgca ctggctcaag
     6601 tccctgccca aaaacatccg tcagaaactg cccccgccca tccaggttgc cggacagttc
     6661 ggcaaacgga ccggtgataa agaccgcgag ctgcctgcgg cgttgggcag gtttttattc
     6721 gaggaatatc atttgacggt gccgtccggc ttctgggcgc cggaaaaact gccgccgcat
     6781 ttacagatcc gcttcagcgt cgttgacgat cacggcggcg aaatccgcag cagccgcgac
     6841 atcggagaac tgcggcagga gctgaccgaa caggtccgcc gcacgtctct ggagagctgc
     6901 cgaaggcaat gggaaaaaga aaacctcacc ggctgggact tcgggcgtct gcccgaagac
     6961 attgaacttt ccggcaaaca cggcgtgacc ggctgcgcct atccggcgct gcaggctgaa
     7021 ggggaagcgg ttcatctgcg gatttttgcc gactcccgtc aagccgctgc ccatcatgcc
     7081 gccggggttt ccgccctcta cggcatccat tttacggaca ggctcaagca gctcaggaaa
     7141 aacctgtcgc tcgggggtga actcaaagaa caggcggctc tgctgggcag cgtccgtcag
     7201 gtggaacgat ccctgctcga gcgggtgaaa aaagatctct tccagcggtc ctggcgaagc
     7261 cccgaagagt ttatgaagca tgccgaagac gtccagcccc ggattctttc ctacggccag
     7321 gaggtgatcg cggctgccgc gccggttctc cggagctttg ccgaaaccca cgagggcctg
     7381 caaaaactga tattcaaaaa ccggtcccat ccggccgtgg caaaatttct gcaggcggcg
     7441 gaggtggaga tgcgcagcct ggttcccgaa gattttatgc tgcgctacga ttttgaccgc
     7501 ctgcgccatc tgccccgcta tctgaaagcc ctcctgctgc gggccgggcg gggaagcctg
     7561 aacctggcgt ccacggaatc caaactgcgg gaagcggcca tttacaccgc gaaacttcag
     7621 gaaatccggg aggcggcggg cgctgaccta tcggaggaaa agcgaaacaa aacggaagag
     7681 ttgcgctgga tgatcgaaga atacaaggtg tctcttttcg cgcaggagct gaaaaccccc
     7741 taccctgttt cacccaaacg gctgaatcaa ctcatcgagg aaatcggaaa tatcctgtga
     7801 ataaaagtgt agggaatggt ttcaaaccgt tccctacagt ggcatgcagg atgatcgaat
     7861 cgaaagtgaa ttcagggaac gctcgcgaac gttccctgaa gaaccccgtt ggcggcgcat
     7921 ggagaggttt agctccggca ctcccggatg gccgcggcaa atcctttcat ggaattccgg
     7981 atggtcgcgt catcgacgca aaaggcgatg cggaagtagc ccggcgtacc gaagccgctt
     8041 ccgggaacga ccagaatatt tttttgcacg agcctctgcg cgaacgccac atcatcggga
     8101 accggacttt tcacgaacag ataaaaagcg ccctgtggtt tgatgaattt gtatcccgcc
     8161 cgggccagtc ccctgcagag caaatccctt ttcttctgat agatcttcac gtccaccgtg
     8221 atgtgctgca gattggccac gatgcgctgc atcagggccg gggcgttcac aaagcccatg
     8281 atccgcgtgc acaaaatcag cgcccggatc aggtcatccg cctcgtgaac cttcggattc
     8341 acggcgatgt agccgattct ttcgcccggc agcgacaggg ttttggaata ggaataggcg
     8401 acaatgctgt tgtcatacgc cgtcagcacg gaaggcagct tgatgccgtc ataaaggatt
     8461 tccgcgtacg cctcgtccga aagaagatag atggccctgt tgtatttttt ttccttttcc
     8521 ttcagcagcc ggaccagtgc ggcaatattc gttttgctgt acacccgacc ggtcggattg
     8581 ttgggcgaat tgagaatgat cgccttggtc cggcgggtga tcgcccggcc gatggcatcc
     8641 ggatccaaag aaaaatcggg gcgcgtggcc gcggttttca ccacgccgcc gtagttttcg
     8701 atgtagaagg ggtactcgac aaaaaaaggt ttgagaatga tgacttcgtc gccgggatcc
     8761 aggatcgact tcaggatcac attgagagcg ccgcccgccc cgcaggacat gacaatgtga
     8821 tcgggatgca ttttcaaacc ggtggtccgg ctgatcttgc gggcgaccgc ccgcctcgtt
     8881 tccggatagc cggcgttggg ggtgtagccg tgcttgagca gaatttcctg acgggcaaca
     8941 cgcaccagct ctttcttgaa ttcgcgggga tggtccacat tgggattgcc caggctgaag
     9001 tcaaatacct tgtcgtgtcc gtgcctgctt ttcagaaggt tcccttcttc aaacattttg
     9061 cggatcaatg acgactgcga catggacgcc ttgattttct ttgaaatgct catgatctct
     9121 tctcccgggc ggctccccga aaggcgcgcc gcttggctaa acgtttttct tcagatactc
     9181 cgaaacctcg cgaaaatcat gaaacgcgcg atagggaacg gcgctttccc gcctgcactt
     9241 cttcaaaagc tcgcccttgg caaaaatcag gtccgcgtat tgggacgggc agatatcgga
     9301 atggccgtcg ccgacataga tgattttttc atacatcagg cggtggtcat ggagaatttt
     9361 actcttgcac gtcccgcagc ggccgcacag ggcattggcc tgtggaaagt caaaggtcag
     9421 cgtatcctcc ccgccgaaag cgacggtatt ggaataaaat tcaatctccc ccagccggtg
     9481 cttgtccaga atcgcccgga tgtaaaaatc aagcccgtcg gaaacgattt tgacatccac
     9541 cccctttttt gcggcgagac gataaaaacg ggaaaaataa ggatcgatgg cggagcattt
     9601 caaaacataa gcgcgcagac gtccggcatc gacccggacc aacggcgcaa tttttccgta
     9661 cgcctcccgc gatcccatac cgcctctgat atatgcgctg tcaatggctt cccggctttc
     9721 tgaggcaaac cggctcaaaa cgccgtctcc aacatccacc cgggaaatcg tgccgtcaaa
     9781 atcgctgagg accaaggcct ttttttgctg aaagagcatg gcaagtattt tttccgcagc
     9841 cgatttttta tcgcttgcgg cgcttcagca gaacaaagac acccagcacg atcaggatta
     9901 ccggcagaat atactgggag tacttgtata aatcaaaccc gtacagttcg atgatgagaa
     9961 aaatgacgcc gaccgtggcg acaaaccagc cttcgacggc acgaatactg taaaacagcg
    10021 tggtgatgcc gataacgatg agcagaatcg gccaggtttg accgaaactg taggaagtgg
    10081 ttttgctgat aaagatcaaa acccccaggg tgattaaaat aagtccgccg gtaagaatgt
    10141 ttctcttttc gttaggcata tccttctcct tctctaaaat gtatttttga gcgggggggg
    10201 gatggctccc ccctgcgcgc gattacgatt ttcccgccag attcaggaaa ggcttgaaca
    10261 aatcgatcgg gatcggaaac accgtcgttg aattattttc cgcggcaatc tgattcaggg
    10321 tctgcaaata ccgaagctgc agcgacatgg gctgcgttcc catcagggcg gccgcttcaa
    10381 tcagcttctg cgcggcctgg aactctcctt ccgcattgat gacctttgcc cgtctttcgc
    10441 gttcggcttc ggcctgcttg gccattgcgc gcttcatctc ttcgggcaga tcaatatgct
    10501 tcacttccac gagcgacacc ttgattcccc aggggtcggt gcttcgatcc aaaatttcct
    10561 gaatattcag gttgatcttt tcgcgctctg tcagaatttc gtccagctcc acctggccgc
    10621 aaacacttcg gagcgtcgtc tgcgcaagct gggacgtggc aaaaagataa ttctccacat
    10681 tcacgacggc ggcgttggcg tccagcaccc ggaaataaac caccgcgtta accttgatgg
    10741 aaacgttgtc cttggtaatg acatcctgcg gcgggacgtc catcgtaatg gtgcgcatgt
    10801 cgatccggac gattttatca atgccgggaa tgataacgat cagccccggc cccttgatgt
    10861 cgcgaagcct gcccagccgg aaaaggaccg cccgttcata ttcattgaga attttgatcg
    10921 cggaagccag aaacataacc accagcacga tcaccaccaa caggggcagc ggtaaagaac
    10981 ccaacataat ccttcctcct tatatcgata atttatttgc cttcttttat ttcttcaacc
    11041 accagcttca ggccttctac cttgatgatt ctgacggggg cgcccttggg aatttcccga
    11101 tcgctcgtcg ccgtccagta ttctccctgg aaaaaaacct tgccgtcgcc gtggatgtcc
    11161 gtcgcggctt ccccccggcc gtcgatcatg gcctccgtcc cggaataatg cttcctcatc
    11221 tgcgccttga cggccagccc gatgatcacg atgaagaatc cggacgccac cagcacggcc
    11281 ggcagcagaa cctgcaggga aattctcagc gccggctccg gcgaatcaaa cagcaaaaag
    11341 gatcccagcg caagcgaaat gactcctccc accgtcagca tgccgtggct catgatttta
    11401 atctccgcaa taaacaaaat gacgctgaac agaatcagca gaatccccgt gtaactcacc
    11461 ggcagcgtgg acagggcaaa gaacgccagc agcagggaaa tgccgccgat gacgcccggc
    11521 aggatcgcgc ccggcgtgga aaattcaaag tacagccccg ccagaccgat cagcaaaagc
    11581 agatacgcaa tatttggatt ggagatcgcc gccagaatcc cctgccgtgt gcccatcttt
    11641 tttgtgtcca aaaccgcatg ggcggtggat attttctttt tggccccggc gacggtcacg
    11701 gttctcttgt caatggcggc cagcagcttt ccgatgtcca cggccacgat atcaatgaca
    11761 ttcagcttca gggcttcgtc ggcggtgatg gactcgcttt tgcgaaccgc tttttccacc
    11821 cattcctcgc tgcgcccgcg cgttttggcg atgctgcggg cataggccgc cgcgtcgttt
    11881 tccacctttt gagacatggt cttgtccatc tcgccgctgc ctcccaggcc gatggccacg
    11941 ggatgggcgg cgccgatgtt ggtgccgggc gccatggcgg cgacatgcgc ggcctgcgcg
    12001 ataatcacgc ccgccgacgc ggcgcgcgcg ccggacggat agacgtaaac gattacgggc
    12061 agcggcgcgt tgagaatgct tttggcgatg tcccgcatcg cggtgtccag gccgccgggc
    12121 gtatccagca gaatgatcag cccttccgcc ttcgcggccg tcgcttcctc caggctctgg
    12181 gcgacgtatt tcgccgaagg agaggtaatc actccgttga gctcgatcac atgaaacagg
    12241 ggtttggatt cctgcgcgaa aacgacgccg gccgctgcga tccacaggag tatcacaaaa
    12301 aagaaacttg tttttttcag catgatgacg cctcttcctt catgaccgcc cgccaggtcc
    12361 gatttctttc atgatcaact ggacatcttc ccaaacctgc ttcttggctg acgggttccg
    12421 caagagatag gccggatgat aggtcggcat gagtttgatc ccctcgtaca actgaaagcg
    12481 cccgcgcaga gcggaaatcg gcgtttccgt ccgcagcagg gcttttgccg cgacactccc
    12541 cagcgcgcag atgatctgcg gagcaatcag gctgagctgc cgcttcagaa aagcctcgca
    12601 cagacggatt tcctccggca gcggggtgcg gttgcccggc ggacggcatt tgaggatatt
    12661 gcagatatac acttcctttc gcgtcagccc catcgcagta atgatgttgg tcagaagctg
    12721 ccccgccttg ccgacaaagg gcaggccctg ttcgtcttcg tccgcgcccg gcgcttcgcc
    12781 cacaaaaacc acctgcgccg ataagctccc ggcgccgaat acgacattct ttcttgtttg
    12841 cgccagagga cactgacggc atttcgcggc ttccagagaa agggcgtgaa acgcctcagc
    12901 gcgatccctg ccgtccgact tgattttcgg ggccgggctg ggtcttttgc agtccacggc
    12961 gacatacaga cggtcggcgt ccagcacctg ccccctgacg gatttcacca gggacagcaa
    13021 atcattgcgg atcgcgtcgg atgaagggga aggaaaggac ttcgtcattt ttttccggcg
    13081 ggtcctttct tcgtttggag cgagcggata tgatccagga tccggtgcgc cgcttcctgt
    13141 ttggtcatct tttccaaagc gatgctatgg ccctgccggt caaggatcgt gatgatgttg
    13201 gtgtctgtct caaaaccggc gccctcgtcg cgcagattgt tggcgacgat catgtccatt
    13261 tttttccgga tcattttctg gcgagcattg gccagcaggt cctgggtttc catggcaaat
    13321 cccaccagaa ggcctttctt ctttttcgcg ccgatcgcgg caatgatatc ggggttgcgc
    13381 acaagctcga cggcggccgg tccccggtct ttcttgattt tctcggcagc cgttatccgg
    13441 ggccggtaat cggccaccgc tgcggctttg atgatgaccg atgctttgga acaggcgtcc
    13501 atgacggccc ggtgcatttc ctgcgcggtg cgcacaaaaa tgacggtttc aaccttcggg
    13561 ggagcgattt tggtcggccc gctgatgagc gttaccttcg cgcctctctg atgggcggcc
    13621 ttcgccagcg catagcccat tttgcccgat gaaaaattgg tgatgtaccg caccggatcc
    13681 aacggctcct gcgtcggccc ggcggtcacc aatacttttt ctccggcaaa atcctggacg
    13741 gtgaaaagcg cttccagctc gtccagaatc tcggggatct ccggcaggcg tccggcgccc
    13801 gtggcgttgc aggccagttc gcccacgccg ctttgcatca cgcggtagcc gatcccggcg
    13861 agttttcgaa ggttttcctg cacgaaggga ttggccagca ttttgtcgtt catggccggg
    13921 cagatcagcg tcggcttgtt ttcggccatg atcgtcgtcg tcagaagatc gtcggcaatg
    13981 ccggaggcga ttttaccgat cacgttcccc gtcgccggcg caatgacgaa cgcgtccgcc
    14041 cattccgcaa gcgcgatatg ggccatgtcg tatggcgccg cgtcctcaaa caggccggta
    14101 taaactgggt tccgggaaag ggtccgaagc gtcagcggcg taatgaactc cgtcgcgctc
    14161 cgcgtcatga tgactttgac ctccgcgccc gctttgacaa aagcgcgggt cagctccgcc
    14221 gatttgtagg cggcaatgcc gccggtgatg cccagaacaa tttttttatt tttcagcatg
    14281 cgtctttcac ttcgctgttt ttcggggaat ataacagcgt ttcccagcaa gatcaaggta
    14341 taaagtcttt ccttacgagg gcaggaaaag gatatttttc ttgcaaaccg ccgtaaaagc
    14401 cattatgaaa accgccaatt caatatcagg attccatcaa ggagcttcgg aaatgaaaaa
    14461 gacggggatt attgcggcgg tgtgcgccat cattctgttg gcgggggcat caggctggct
    14521 ttattttgca ggctggctgg atcgggaaaa acccgcgatt caactggatt cggatatttc
    14581 tgccatcggc aggaaaaagg acatcggcat cacctttgcc gacgagcgaa gcggcctgtc
    14641 gggcctgaag gtggagcttg ttcaggacaa caaaccgcat cttctcgccg aggaaacatt
    14701 tccgtcccga agcaccaggc aaaaggtcct tcgcctttcg gtggagcccc tcgcgctcca
    14761 gctgaacaac ggcccggccg tcgtccgcat cacggcccgg gacgcttcct tttttaaaaa
    14821 tgaaaccgtc gtctccgagc agacccggat cgatacgctg ccgccgcaag tttacgtgat
    14881 gagccccgtc aactacatca accagggagg cgccggcttc atcccgtacc ggacgtccaa
    14941 accggccgcg ctgaccggcg tcttcgtgga cgaccggttt tacccgggat acaacatcgt
    15001 catcggcggc aagcccacgt cggtggcctt cttcgccctc ggaaccggcg cttccgcggc
    15061 gaccaaaatc gtcattttcg cccgggacaa cgccggcaac gaagctcgaa cctccatgcc
    15121 ctttacgatc aagccgaaga aattccgcgc ggacaaagtg aatttgagcg aaacctttct
    15181 ccagaaaatc atgcccgaat tccaggcgtc gattccgcag ctgcagggga agacgcccgt
    15241 tgaagtcttt ggctatgtca acacaactct ccgggaagag aacgccaaaa ccattcaatc
    15301 cgtctgccgc aagtccatca ataaaaaatt atgggaaggc gcgtttcacc ggatgcgcga
    15361 cgccgccccc atggccctgt tcggcgatca gcgcacctac ctggtggacg gcaaaccctt
    15421 cggcaactcg gtgcaccttg gtgtggatct cgcctcgacg gcacacgctc ccatcgaagc
    15481 cgccgccaac ggaatcattg tcttcgcggg cgcgctgggc atttacggca acgccgtgat
    15541 catcgatcac gggttgggcc tgttcagcct gtacggacat ctttccgcca ttgaaacaac
    15601 cgtcggcaaa agcgtcaaaa aagaagaaaa aatcggcgtc tccggactga gcggactggc
    15661 cggcggcgat catctgcatt tcagcattct ggtgggcggc gagtttgtca acccgcagga
    15721 atggtgggac ccgcactgga ttgaagacaa cgtgatgaaa aagatgactc tataaggcta
    15781 tgggcgaggg gccatcggcg agaggcgaga tgaaatgggt tcctatcgcc cgttgtcgat
    15841 acgagagctt tgaagaacgg ccgtttttgt catttcgacc gaagggagaa atcttgaaca
    15901 aacggccatc cttaagattt ctcgtcgctc acgctcctcg aaatgacact tttcaaagct
    15961 ctcgatacac ttccgcgcaa attgcctgaa cttcctccct cataagccgc cgccaaaggc
    16021 ggcgtgaagc gcgattccct aactcttatt gatgtcgcgg tgggtcgtgt gaacgatgaa
    16081 tttctgcttc tggaaaaaca ggctgaactg attggccatg acgacggacc gatgcttgcc
    16141 gccggtgcac ccgaacgcaa tgttcaaccg cacctttcct tctttttcat aaagcggaat
    16201 gatgaagttc agcatttcgg atgctttgac gatgaaggtt ttgcattcct cattgttcaa
    16261 aacgtacttt tccacgtccc gatgatggcc ggtcatgttt tttaactttt ccacataaaa
    16321 gggattgggc aaaaatctca cgtcgatcgc caggtccgca tctgccggca ggccgtaccg
    16381 gtaaccgaaa gacatgacgc tgatcatcat tttcttccgg gtggaagaag gcaaaaactg
    16441 cttctgcacc gtgtctttga gctgatgcac attggacgtc gtggtgtcga tgacgatgtc
    16501 ggccatttgc cgcagggtcg ccagcttttc tgtttccgcc tgaatgcctt ccatgatgga
    16561 acgcttttcg gacagcggat ggctgcgcct cgtctcgctg aaacggcgca gcagagactc
    16621 ttctccggct tccagaaaca gtattttgat ccggtatccc ttttccttca ggcgttcaaa
    16681 gatgcgctgg tacttttcca gaaagctttt ctgccgcaaa tccatgacca gcgccacctg
    16741 tttgatctgc ggagaagact cgagggtgat ggagaggaac ttcggcagca gcacaaccgg
    16801 gagattgtcc acgcaaaaga agccgatgtc ttccagcgcc cggagcgcgg tgctttttcc
    16861 ggaaccggac aatcccgtga tgatcaccac ctggagattt ttcaattatc cgacctcgtt
    16921 cagcttttgt atggctctcc ctaacagatt ttggcgcaaa tatccagtcc ggggaatgcc
    16981 cacccggaca agcggcagat ttgtttccag gattcgggtc tccagcggcc gggaaatttc
    17041 ctcccgggta gctcgaaaga catcgatgac gaaggcaagc tccgcctttt tccgccgccg
    17101 cgatccgtcc agcatccggt ccgcggcggc gatgccggcg gcctgggtat ggatatattg
    17161 ttgaatgccc gggtggccgg aaatcatcag ccgtccctga atacttttga tcacggccag
    17221 atcgtcggcg atccagtcat agcctttgcg gacggccgcg agcgcggccg tcgttttgcc
    17281 gatgccgctg ggcccggtca ggaggatgcc ccgttctccg cacgccagcg cgacgccgtg
    17341 cagggtcacg caccggtgaa ttttctcgcg gaggatcgac cggatccggc tggccagcag
    17401 gttttcatga agcggggaag cagccgcggg aagacgatgg ctttgcatct gccctctgag
    17461 caaaacgggc agggacgcgc tttgggaaaa aatcagcaaa gccggacggc gtccggccat
    17521 tcgggagaaa tatttctcgc gggacggcgc ggaaagcgcc aggaggtttt ttgccgtcgc
    17581 gggcggaaca agaagaatcg cggattcccg ccccggcgcc gcggcgagac tccgcaggca
    17641 ttgcggaaaa tgcccgggaa aacggatctt gtgatgttgc ggcccgggaa ttctccggag
    17701 gcccagataa ggcagacggt cctgaaacag ttggccggtt gtcgtctccc gatgccgaga
    17761 gagccggcta cagcggacag gatgcgtcct gctcaacgat ggctttgtac aaatcgtttt
    17821 tggttttcgc atcgataagc gttttacgga agttgggcgt ttttaacatt ttggaaattc
    17881 tggccagcgc tttcaaatgc tggcctgtgg aatgctccgg cgccaaaagc agaaaaaaga
    17941 gccggaccgg cttcccgtcg atggcgttaa aggcaattcc ccggcggctc cgtccaaagg
    18001 cgatcaccat gtcctgcaga tcggaaactt tgccgtgagg aatggccacg ccgtcgccga
    18061 tgcccgttga acccagtttt tcccgctgct gcagcacatc gatcgccctg gcccggtcaa
    18121 tcttcaggcc gctctcgatc agaatattga tcaattccgc aagcacgcct tccttggtgt
    18181 cggacagaag gttttcgttg agaaactcca ttttaaagat ttgatccagt cgcatgcgca
    18241 acacctgacg ttgaaatccg ggacgctgtt ctgatgtctc aaccgtccat catcctgatg
    18301 gggcgcggca tttttgtgcc gcaggtccga gatcggcgtt tttgtaaatt aactgttcgt
    18361 ttcaatatgg gcgaacttgc cgtcgtccag acggtgcacc aggctcactt tttccgaagc
    18421 cgtatcccga tagatcagaa aacgcgcctt gctgctctcc aattcgagaa ccgcttcctc
    18481 cagcgacatg ggtttcagaa tgatcttgcg ggtttcaacc actttggagg aagcatcgtc
    18541 ctcctcaggg ccttccaggc tttttccgcc ggggtgacgg atgcttttgg gtttgtgctg
    18601 cctgtttttt tctttctgct ttttaagctg tttttcaatt tttacaatgc aactgtccac
    18661 tgccaggtgc atgtccttgg cttcttcttt tgcgttcaca ttccatccgt tggcggtcag
    18721 gttgatttcc acaacattgc gaaacttttc cgtggacagg atcatatgga cttcggcagg
    18781 cacatcgaga tattttttca atctggcaat cttttcctct gcataggctt tttgccaatt
    18841 ctcaccttcg cccgttctga aagttaccga aatattcatc cttttcctcc ctcctgagtt
    18901 taggccgtct aaaacatctg aaacacgcct tcacggccgg caacaaaggt tagtcgtata
    18961 gtggcgacaa aaccaaatgt caaccggaat tttaccgacg gttttgatta tatctctatt
    19021 tcaaaccgtc tgcttcaaaa atattttttc cgtttcgacg acggcaaaat gccagtcatt
    19081 tcgcgatatt tggcaacggt tctcctggcg atatcaatgt ttctggcctt cagcagcttc
    19141 acaatttcac aatcgctgta gggttttttc ggatcttccc tgctgataat ctgccgaatc
    19201 tcctccttga cgcttttgga ggcgatattt tccccggacg ttctctgaat ggagctgccg
    19261 aagaaatatt tcatttcaaa gatgccgcga tgagaatgca tgtacttgtt cgtgaccacc
    19321 cggctgatcg tcgattcgtg catttccaca tcgtcggcga tgttccgcag caccagcggc
    19381 ttcaaatagt caatgccttt gtcaaagaaa tctttctgat gtttcacgat gctttcggcg
    19441 accttataaa tggttttctg cctctgctga atgcttttga tcagccaggt cgccgactgc
    19501 accttttccc ggatatactt cctgccgttt tccttttccg cggagtcctg ccctccaatg
    19561 ccgcccatga tttcacggta aaaattgctg atccgcaacc gcggcaaccc gtcatcgttg
    19621 agaacaatct tgtattcgtc tcccgatttg accacataca catcgggaat gatggattga
    19681 accttttctt ccgaatagac ggcgcccggt ttggggtcca ttttactgat cagcaaaacg
    19741 gccatttcca cttccttcag gggaactttc agcctgcggg caatctgaag ataattcttc
    19801 agctccagtt ccttgagatg ctcccggata atggtttcga caagcggatt ttccccggca
    19861 aacattccgg cctgaatcag aagacattcc tgaagattgc gggcggccac gccggcggga
    19921 tcaaattcct gcactttttt tagaacgttt tccaccaggt cttcgccgac gttttccaac
    19981 cgggcaatct ccgccacggt ggcgcacaaa tagccgttgg catccagatt gccgacgatc
    20041 tgctgcccga tgcgcacctc ttcttcggta aacggagaaa gtttcatctg ccacatgaga
    20101 tgctcggtca gcgaagggct ttccgtcagg acgttgtcca gcgtcggggc gtcgccttcg
    20161 gcccggtcgt acgaaacgcc caccggtccg taatcttcca gataattgtt ccagtcgaat
    20221 tcttcccttc cgtccccttc gccggtcagt tctgcggtat gctccggaat cttgctttct
    20281 tccttctcga tggactgaac gtcctcttcg acttccgcgc ggatttcctc ggcgctggcc
    20341 tcgtccggcg ccgcctcctc cagcaggggg ttttcctcca tctcctggtt gatcgtatcc
    20401 accagctcct ggcgcgaaag aatcaggagc ttgatcgcct gctggagctg cggcgtcatg
    20461 atcagctgct gggacagctt cagattttgt cgtaattcaa aagccatgat tgaaaattgc
    20521 gcatccgttt gtcgctacaa actgaaatcc tcgccgaaat agaatttttt ggccagttca
    20581 ctccgggcga tgacgtccgg ctggccctga accagaattg cgccctcatt gatgatatac
    20641 gcccggtcgc agcaggccag cgtttcccgg acattatgat cggagataat gattccaatc
    20701 cctttatttt tcaacttttc gataatcttc tggatatcgg ccaccgccag cggatcaatg
    20761 ccggcaaaag gctcgtccag caggatgtat ttcggcgacg ttaccagcgc ccgggtaatc
    20821 tccacccttc ttctttcgcc gccggacagg gaataggcgt ggtttttcgc gagcgaagtc
    20881 agatccagct ctcctaaaag ttctttcagt cgttgccgcc ggtcctcttc gctgatctcc
    20941 agtgtctcca gaatcgccag aatgttttcc tcgacggtga gcttgcggaa aatcgacggc
    21001 tcctgcggaa gataattcag ccccttgcgc gccctcttgt acatgggata ggcgctgatc
    21061 tcctcgtcgt ccagaaagat ttcaccgccg tcgggcttca ccagcccgac aatcatataa
    21121 aaggtcgtcg tttttccggc gccgttcggt cccaggagtc cgaccacctc ccccggtgag
    21181 atggccaggt caatctggtt gaccactttt ctcttattgt aaatcttgac cagtcccctg
    21241 gcggataaat ggctcatctg ctatcccggg gcgcccgtcc ggcgcccgtc cgattatttt
    21301 tgtttccgcg ggtggatgat cgccgtaacc cttccggaat tttcgtcggc gcactgctcc
    21361 actcttcccc ggttctcatc gacctgaatg atgacccggc aacccttgat cacgcttttt
    21421 tcctgctgca ggacgggatt ccccgtcatg acaaaccggg cggtgtcctg ataataaacc
    21481 gcttcatttc ccgtggccag cacatctttt tgcgtaatca ccacatggcc gcttgccaca
    21541 attttttcaa ggtctcccgc ctgctccatg cccggatcga cctttttttc tttcgaatcg
    21601 ccggaatcct tatagtaaat cgacaaacgg tccgcctgga ttcggacatc cccctgctct
    21661 gccacggcat tgccggaaaa caccaccatc cggctggcct gaaacgcttc cattttatcg
    21721 gaaacaatct gaatcggctc gtttttgcgc agcggatttt tcgaaccgcc gccggtctgc
    21781 gcgacagcct gaatattcaa ggcgaacaga aaccccaaca cgatcagacc tgtctttttt
    21841 atcggcatga atggccctca tcagtaaatt ttcgctttga cgccggaagc aagcgcaagt
    21901 tctcccttgt ccatcagaat ggaaaggccg accccctgaa ttttcattcg gctcgtttcc
    21961 atccggacgg gagcgtccgt ataaatttct ttccgggcgt cgctgtaccg caggacatcg
    22021 gtggaaaatt tttcccccgc atcggccgtg accaccacat gaccacggat ttcgacgtct
    22081 ttggtgtccg tccgcatctc gcccttgtcg cccgtcatca caaaggtctt tcctttttgg
    22141 gtgaccagtt tcatctgaac ctgatcaaac agggccagat tctgcttttt ctgatactgg
    22201 gcggttttcg cctttacctc ccagcggaga tcatccttgc cgacttccgt ataaaagaaa
    22261 tccgcaatgc gaacatccgc ttcgtcaggc aagatcttga ccagattctc ttttttgccg
    22321 cccggcgccc gcagaaaagc gtacagcaca gctcctgcaa gcacaagaat catcagcgca
    22381 atcgcagcga tttttgcttt ctttgaaaac cgcatggttt tgtatatcac ccgccttctt
    22441 ccgtgtaaag cgattcgggt gcggcaaaat cgtagcgccc ggcaatttcc ggccagaagc
    22501 cctgtgctgt aagcagcatt tcgcagattt cccttaccgc gccctgtccg ccgcggcaaa
    22561 ccgtaacgta atcgacctgc ttcttcacct catctgccgc atccgccacc gcagcggaaa
    22621 agccaaccag cctcaggacg ggaatgtcca caatatcgtc gcccagataa gcggtttgct
    22681 ccggcgtcag gcaatttttc tcgaggatcc gggcaaaaac atcctttttg ttcaaggccc
    22741 cctgatacac ttccgtaatt tttaaatcgc gcgcccgatg atccaccacc aaagacgtcc
    22801 ggccggtcag aatcgcaacc tgaatgccgt ggcgctgaag catcaccaga ccgtgcccgt
    22861 cgcggacata gaagtttttc atctcccgcc cgtcgctgtc cataatgatt ctgccgtccg
    22921 tcatgacccc gtccacatcc agaatcagca tctttatctt ttcaagcttt ccccgtat
//
LOCUS       DAOSRM010000023        22921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2426_length_22921_cov_97.666142, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000023 DAOSRM010000000
VERSION     DAOSRM010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 22921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22921
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2426_length_22921_cov_97.666142"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..892
                     /locus_tag="PLA58_03820"
     CDS             317..892
                     /locus_tag="PLA58_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR61954.1"
                     /translation="MSKTDKRADIIEVALNLIAERGFHDSPMSLIAREAGVAAGTIYL
                     YFESKDDLILALTRNLEDRINEMIQDGYPVEDSIREKFLYMNRKILKYFMEHPLHFRY
                     MEQFFNSPYGVSHRKERLLDKSDRNKMMTDIFEHGVDEQVLKALPMPALFALAFGPIP
                     TLVRNHILGFFYLDEATIEKVAQACWDAIRK"
     gene            1046..2245
                     /locus_tag="PLA58_03825"
     CDS             1046..2245
                     /locus_tag="PLA58_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOR61955.1"
                     /translation="MQIHTMAVRMIVISSLLAAGILLNGCSDKGKGGPPPPPEVSVVT
                     IQTKPVPITTELPGRTSAHLIAEVRPQVDGIIQKRFFTEGTNVRAGQVLFQIDASLYQ
                     AVFDNAKASLARSEANLSTIQLKAARLKELLAEKAVSQQDYDDAAAALKQTLADIQFG
                     KASVEAARINLQHTTITAPISGRIGRSNFTEGALVTTHQPQPLATIQQLDPMYVDVTQ
                     STAEILRLRRQLQEGRFEISKKNLGGVRLILEDNLEYPWTGTLQFQDITVDPTTGSVI
                     LRMVFPNPKGILLPGMFIRTVIQEGFNKQAILVPQQGVFRDPKGNPFTLVVARNGKIE
                     QKPLTLDRAIGDAWLVTSGLAPGDRVIVEGTLKVKPGMIVKAVPFGQASQHDGRAKTP
                     APPGKTN"
     gene            2261..5509
                     /locus_tag="PLA58_03830"
     CDS             2261..5509
                     /locus_tag="PLA58_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOR61956.1"
                     /translation="MLSKFFLDRPVFAWVIAIIIMSVGILAIYNLPVSQYPQMAPPSI
                     AVTAYYPGSSAETVENSVTQVIEQKMTGLDNMIYMSASSDSAGYTRLELTFAPGTDPD
                     MAWSKVQNKVQAALTSLPDMVVSQGVDVRKSTRNYLMFVGLISEDGSMDGMDLRDYAK
                     SNLEKILARVPGVGEVEVFGGGYAMRVWLNPKKLTDYHLTVEDVIGALKAYNVEVSAG
                     QFGGAPSIPGQRLNATIIVQNMLSTPEEFASIPLRTNPDGSIVRMGDVGRTELGTEIY
                     DIQVSNNGRPSAGIAIRQSPDANALDTADAVKAKMAEMSRFFPAGMKVVYPNDSTIFI
                     KVSINEVIKTLVIAIFLVFLVMWLFLGNMRATLIPTIAVPVVILGTFAILGLFGFSIN
                     MLTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLPPKEATAKSMEQITSALVGIGLVL
                     SAVFGPMAFFPGSTGVIYRQFSITIISAMLLSVIVALILTPVLCAALLKPVKAGHEPA
                     ENAIFFLKPFFMWFDRVFFKLRDRYVGMVDHTLSKKKRYLVVFLVILVVMAVLFLRMP
                     TAYLPDEDQGTLLAQVIMPSGSTLEQTNEVTKQIEKYFQENEKEAVEMVMAITGYGFS
                     GRSQNNGMVFIRLKDWHLRDRSDLKAAAIVKRAMGALSTIRNAMVFTFQPPAVIELGT
                     AIGFDFQLLDRGALGHQALMDAQNQLLGLVAQDPRVMKVRPNSMADVAEYRIDVDWEK
                     AGALGVPINSIHTTLSAAFGSAYVNDFVQGGRIKKVYVQADAPFRMLPKDLEKLYVRN
                     TQGKMVPFSSFATGRWTSGSPRLTRFNGFPSINIWGEPAPGRSSGEAMQAMEEAAAKL
                     PQGFGFDWSGLSYQERMASSQAPLLYSFSIFVIFLCLAALYESWAIPIAILLVLPLGV
                     IGGIMASSFRGLANDVYFQIGLLTTLGLTTKNAILIVQFAKGGLEKGMGLLEATLEGA
                     KLRFRPIMMTSLAFGFGVLPLATTTGAGAGAQNAIGTSVLGGMITATLLAVIFSPLFY
                     VLVETLFGKKPKTDTGGPSVFNRLLEKVLPKRLKQKMTAADKTHASEEQPHE"
     gene            5502..6896
                     /locus_tag="PLA58_03835"
     CDS             5502..6896
                     /locus_tag="PLA58_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOR61957.1"
                     /translation="MNKKRLLILMVLLAGCTLAPKYDRPEAPVPKTWPSGPSYQDTKQ
                     APQASQLPWRDFITDPRLREVIAVALANNRDLRVTGLNVEKAKAMFGINRASLLPIVN
                     ATGNWYQERVPADLSSSGKIYTAEKYSVNLGLAAWEIDFFGRIRSLKDAALEQYLATD
                     EARRSAQILLVATVTQAYLALAADREALQLVASTLEAQEAAYNMIRKRHEVGMASELD
                     LRRSQTQVETARGDVARYTQLVAQDENGLNLLVGSSVRPDLLPRDLSGVTLPRDISAG
                     LSSDVLLGRPDVLAAEHFLKAANANIGAARAALFPRISLTTSFGTASADLSGLFKGGS
                     DVWTFAPQIVTPIFDPRLWYAYDVTKIEKEISIAQYEKSIQTAFREVADALAEKGTVS
                     RRVTAQQSLVDAMAVVYRLSEMRYEKGIDSYLSVLDAQRSLYGAQQGLILLRLASVNN
                     IVTLYKTLGGGASS"
     gene            6893..7573
                     /locus_tag="PLA58_03840"
     CDS             6893..7573
                     /locus_tag="PLA58_03840"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR61958.1"
                     /translation="MTGVPAKEIKFFLLNHVLTSVIFFTLRLYAKTLRVQIEGEEKIL
                     EYLAREGTILFACWHQRLFGGFFVPRFFQWKPCIMISQSRDGDLISKIVSRLGWIPVR
                     GSSTRGGARAFRVMAQNVKKNRIGAHIVDGPTGPARIVKPGLTALARSANAVICPAVV
                     SYQSAWTANSWDRFLVPKPFTRVLIRFGSFFNVPDDADRNLLEAIRLQVEQHMIEAYE
                     AADSGWGK"
     gene            complement(7636..8361)
                     /locus_tag="PLA58_03845"
     CDS             complement(7636..8361)
                     /locus_tag="PLA58_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOR61959.1"
                     /translation="MLVKSKTSAHSSHQGVCLSCGAGNIGKRRYCSIRCRQQLRQKLN
                     TRSGLLQALNTRYATFYFTDTLIVLDVVPHGIREVFRYKARRTAGVKPAEDFGAMSNR
                     LGDAWWAEEKRTTKNYLASRRVLEMAERLAMAEGLKRPRWVKVPGVKPESILLLDMAK
                     ADLASREPHKIIKNAYRRQVKIHHPDAGGTAAAFRRIHAAYQDLLNWAEHPTFIRHRG
                     FPDKWYYDGDHKRWIQPVPLKKG"
     gene            complement(8425..9036)
                     /locus_tag="PLA58_03850"
     CDS             complement(8425..9036)
                     /locus_tag="PLA58_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOR61960.1"
                     /translation="MIEKNVKTDFCANVSEPVIGEGTFVHPLAAVIGNVELGKNIMVS
                     PMASVRGDEGQPLHVGDDSNIQDGVIIHALETELDGKPVEKNRVDVDGKKYAVYVGNR
                     VSLAHQAHIHGPAVVMDDTFVGMRALVFKTFVGKNCVIEPGAILIGVRVADGRYVPGG
                     SVVKTQADADALPVITADYPMKEMNKGVLHVNKALAKGYLAAK"
     gene            complement(9105..9734)
                     /gene="coaE"
                     /locus_tag="PLA58_03855"
     CDS             complement(9105..9734)
                     /gene="coaE"
                     /locus_tag="PLA58_03855"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOR61961.1"
                     /translation="MLNVGLTGGIACGKSTVAEMFVRLGGRLIDFDKLAHEAQEPGKP
                     AWQDVVHCFGSEILLPDQTIDRGKMADIVFRSAERLRRLNAIVHPRVFEEWWSRLEKI
                     KAQAPHAIVFSDVPLLFEGGMQHLFDLTILVKIEPGQQVERLMARSSVCREDAELRLA
                     NQMPIYDKIQLATIVIDNQCVMEETERKVAAVWEDLLAREKAKALQAED"
     gene            complement(9738..10514)
                     /locus_tag="PLA58_03860"
     CDS             complement(9738..10514)
                     /locus_tag="PLA58_03860"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOR61962.1"
                     /translation="MLLVIDVGNTNTVLGLFDKDQLLHDWRIRTEIDHTIDEYGVLIY
                     NLYQSTRMRAKEIKSVRAIIISCVVPPMLNILEPLCVKYFNIIPMIVGPGIKTGMPIF
                     YDNPKEVGADRIVNAVAAYDKYRQESIIVDFGTATTFDYVSPRGEYMGGCISPGIVIS
                     SEALFEKASKLPRVEFGKPKTIITKDTVSAIQAGIMFGYAGLVDGIVNRMKAEVKTNP
                     LVIATGGLARVVAQEAGTIDKIEEMLTLDGLRIIYERNKK"
     gene            complement(10521..11312)
                     /locus_tag="PLA58_03865"
     CDS             complement(10521..11312)
                     /locus_tag="PLA58_03865"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOR61963.1"
                     /translation="MPLNEASLKKHLAGKFIGHSLRYYEEIGSTNDEAFRLGVQGAPE
                     GTALIAERQSAGKGRMQRAWHSPPGANIYTSVLLRPRFEPDLAPQISLAAGVAVAETL
                     DPYCPGKVFLKWPNDVQIGGKKVCGILTQTKTAAGVIDFVVVGIGVNVNWNLKEFPED
                     IRKIATSLSVEAGREVSRLELIILLYENLAKWYRELAQNGFAPLRKKWLERTTMIGKP
                     VSVTFGKETISGEAADLDEDGSLVILADGNQKVKVAAGDATVGKR"
     gene            complement(11409..12242)
                     /gene="nadC"
                     /locus_tag="PLA58_03870"
     CDS             complement(11409..12242)
                     /gene="nadC"
                     /locus_tag="PLA58_03870"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOR61964.1"
                     /translation="MIDSRIQKIIRAALAEDIGTGDITTQATVSPKKKGRAVAVAKEN
                     FVVAGLEVFEAVFQCLDPDIVVRKVVADGSRVRKGAVLARVEGRLSAILQAERTALNF
                     FQRMCGIATLTAAYVDAVGKTKAKIYDTRKTVPGLRALDKMAVRQGGGTNHRFGLFDA
                     VLIKDNHIEAAGGIAAAVAARKKHWKRSFKIEVETKNLREVKEALVCGADIIMLDNMT
                     IHAMKKAVDWVAGRAFIEASGNVTLKRVREIASTGVDGISVGALTHSVRAADISLKIT
                     G"
     gene            complement(12320..14977)
                     /locus_tag="PLA58_03875"
     CDS             complement(12320..14977)
                     /locus_tag="PLA58_03875"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOR61965.1"
                     /translation="MAMKELGKAFEPADAEKRLYDYWLKNKFFAAKDKSESPAFSIVI
                     PPPNVTGMLHMGHALNNTLQDIIIRFKRMQGHNTLWMPGMDHAGIATQNVVEQQLRKE
                     GLSRHDLGREKFIARVWEWKEKYGGVIINQLKRLGCSCDWDRERFTMDEGLSQAVREV
                     FVRLYNEDMIYQGDYIVNWCPRCHTAISDLEVEFKSDQSNLWNIRYPFADGKGEIVVA
                     TTRPETMLGDTAVAVHPDDPRYRDKIGKKVILPLMNKEIPIIADDYVTMDFGSGAVKI
                     TPGSDPNDFAMAQRHHLEIISVIDGEGIINEHGGKYKGQDRYEARASVIADLEKGGYM
                     VGTEPYAHNVGQCYRCKTDIEPMVSKQWFVKIKPLAAKAIAAVTKKKTRIVPSSWEAT
                     YFDWMSNIRDWCISRQLWWGHRIPVWYCGGCGKVIVSTVDPDKCPVCDGASLKQDEDV
                     LDTWFSSALWPFTTLGWPRRTQALKTFYPTSLLITGFDILFFWVARMMMMGLYVMKDV
                     PFKDVYLHALVRDEKGDKMSKSSGNSIDPLVMIDRYGTDAFRFTLAAYTAQGRDVRMS
                     PERIEGYKFFVNKIWNAARLTLSNLADYDENLSRNAPDALPEKWIKAKLNETIEEVTR
                     SLDEYRFNDAAASIYHFIWHEYCDWYLEVSKPALYGKISPERKLATQRTLKEVFGAMM
                     QLLHPFMPFVTEELWQALSGSDENSIMVSRFPAVREEWSEKEAEKEMEMLMDIITSVR
                     NIRGEMRIPPSVKLKVMISAADGATRASVENGAGYIVNLANLESLTVEENLVEPKGVA
                     TGVIGSTKIFVPLAGIVDIAGEKARLEKELAKVSKDLEQSSRKLANHDFRDKAAPEII
                     EKEEGKLKSFQEKFAALEGALKKLKEMNL"
     gene            complement(15124..16329)
                     /locus_tag="PLA58_03880"
     CDS             complement(15124..16329)
                     /locus_tag="PLA58_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61966.1"
                     /translation="MFCRANMKKVLFAIILFSAVLLIAACGTKARQPSNALDTPEHHV
                     FTGMKLIDQEKYSEAEREFTLALELNDKYSKAYTGMGLAKAYQQNYTAAFGELEKAWG
                     TAKAADEQLAVHIGYIRVNTLSKVACMKIGVDCEQDKSWLKKCVSSYEEAVKLDPSSS
                     SAPYFMGLAYKTALDYGKAGAMFAKVLEIRKEYVSQANAQWNLVQKIQRAMPGTVTGK
                     KIALLESISRADAAALFMEELKIDVLYKKRTPKTFDTSFKDPEKAARSAGPAALTAKD
                     IANHPLKADIEGILAIGVRGLEINPQGNFEPAGALNRAEYALMIEDILIKVTGDNALA
                     TKFIGSTSPFPDLRSDLPYFNAVMVVTSRGIMEATDITTGEFAPLKPVPGADALLIIR
                     KIKEELKFD"
     gene            complement(16430..17386)
                     /gene="miaA"
                     /locus_tag="PLA58_03885"
     CDS             complement(16430..17386)
                     /gene="miaA"
                     /locus_tag="PLA58_03885"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOR61967.1"
                     /translation="MKSNDCSNESKTPGIPLVVIGSPTATGKTRLALQLAESLGAEIV
                     NADSLQVYRYLDIGTAKPTREERNRVRHHLIDVVNPDEEYNAALYSEQARGIIAKLAG
                     EGRPALVVGGTGLYIRALLQGIIDTPPVDENIRRHYKELRDRYGRAYVFGLLRKRDPL
                     AADRLNPNDSVRVIRALEVLDQSGQSILELQKKHRFADCPYTVLKIGLCVERDELKKR
                     IHRRTEQMVEAGLLDEVRGLMDRGYSEKLKPLQSLGYRQVLSFIQGRCGWDQAIAEIN
                     HETWQYAKRQMTWFRADKTIAWYGPEETQKIEGQTRKFLAQY"
     gene            complement(17355..19148)
                     /gene="mutL"
                     /locus_tag="PLA58_03890"
     CDS             complement(17355..19148)
                     /gene="mutL"
                     /locus_tag="PLA58_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOR61968.1"
                     /translation="MSRRIAVLSEEVANQIAAGEVVERPASIVKELVENAIDAGGRDI
                     RVEIEKGGCRSIRVSDNGSGIEREDVALVFERHATSKIVRIDDIYDAGSFGFRGEAMA
                     SIASVARVELLTRRADDLAGTRALVEAGTISEIAPAGLPPGTQITVTELFANVPARRK
                     FLKTEATEQSACLDAVTRLALAHPEIRFSVRADGRDVFAAPAAAGVADRVAMILGKDF
                     TDHRLTIAENKENIELRGFVSTPAWTKSNSKSIFWFVNSRFIRDNSMMHALLAAYRQV
                     MEPRRFPAAVLFLRLPGEEVDINVHPAKLEVRFKNSRGVYDLVSGAVLRALAGAQASR
                     DAVAYRLAPREAASASSGFWRSSPYRASSDRPQDVFSRQNLQQAIGGDLFARAGEPDV
                     PSHVEDASCEERITFDHRTYLGQFAGTYLVFGSPGGLMLLDQHAAHERILLERLKASE
                     MERAASQPLLMPEVVTLTPAQISLLEEALPLLAGIGLELDIFGRDAVVVKAMPAALPN
                     VEPRGLVSDLVDQLSDGQAAPSLAERKEKILASLACHAAVKAHADLSSGEVAALCRDL
                     EKTPFNATCPHGRPICVQFTLYEIERLFKRK"
     gene            complement(19235..20140)
                     /locus_tag="PLA58_03895"
     CDS             complement(19235..20140)
                     /locus_tag="PLA58_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61969.1"
                     /translation="MKLRFVSITVLTGILLVWTSAAFCQNAISMSEWVEKVGQGSINW
                     SAGFIEAKGIGAPPDRYLGKINARPMALRAAKVDAYRNLLEITKGVQVDSRTTVKDFA
                     VESDVINTQVEGLVKGAQIVDQQYLSDGTVEVTLRMPLYGELSKIMIPISIQKRKEIP
                     PPPPVVEAPAPPPAPVVAAPAPPPPPAAVAYTGLVVDARGIQARPAMSPRIYDEDGKE
                     VYGSANVDREYAVQQGMSGYARDLTAAQSNQRVTASPITVKGLKTGGAGRSDLVISNA
                     DAQLIRASAENASFMKKCRVMIVLD"
     gene            20403..>22921
                     /gene="ileS"
                     /locus_tag="PLA58_03900"
     CDS             20403..>22921
                     /gene="ileS"
                     /locus_tag="PLA58_03900"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOR61970.1"
                     /translation="MDYKKTLNLPQTDFPMKANLAGREPEMLKRWEEMDIYHKIRECA
                     KGKKPYILHDGPPYANGNIHLGTALNKIIKDIVIKAKNMTGFDGVYVPGWDCHGLPIE
                     HQVDKELGGKNSGLSQVEKRRACRVYAEKYVGIQRDQFKRLGVFGEWGNPYLTMAYPY
                     EAATVHEFGKLFLNGSVYKGKKPVYWCATCKTALAEAEVEYADHHTPSIYVRFAFTSD
                     IGAIRPKLAGKKVFMVIWTTTPWTIPANLAIAVKDDFIYAAVKIDDDVLIVAKDMLDY
                     CLDAFGHRGKACEIVDEFKGEVLEGQKCVHPLIQRNSLLILAPFVTLEAGTGAVHIAP
                     GHGQEDYEIGLEYGLENYAPVDDAGKFTEDVEHFAGQFVFDANDNVIQKLKEAGALLG
                     HVPMQHSYPHCWRCKKPIIFRSTEQWFISMEKNDLRNKALAAINEVQWIPSWGRDRIY
                     GMVENRPDWCISRQRLWGVPITIFYCAKCKNELLTQDILNHLVSLVEKNGADVWFEKE
                     PKDLMPKHTVCPHCRSTEFTKEVNILDVWFDSGVSHAAVLEKRPELGSPCDLYLEGSD
                     QHRGWFHSSLLESVGTRGRAPYKSVLTHGFVVDGEGKKMSKSGGNAIGAEDAVSKYGA
                     EILRLWVAAEDYTDDIRISEEILKRLMEAYRRIRNTSRFILGNLYDFAPDADRLDGDQ
                     MLEMDRWALHRLQEVIRRVTEAYERHQFHMVFYTLYNYCTVDLSALYLDVLKDRLYTS
                     KTASAARRSGQTAMFIILNAMTRLLAPILTFTAEEVWAALPAWNGKEESVHLTQFPQV
                     EEKYFNASLGERWKAMIDAKSEIAKAVEQARKEKVIGHSLDA"
BASE COUNT         4883 a         6742 c         6181 g         5115 t
ORIGIN      
        1 tttggctatc gcaatcctcc tcatgtcgat ggtatccgcc ccgctgatcc gggttctccc
       61 gggcctgccg gcgtctgccg cagagcggga aacccccgcg tggccaggcg agccggccgg
      121 gggccggccg ctcaaattct gccggaaaat aaatcgtcaa cgacgctttc atagcctgcc
      181 ggagtcgaaa attgtaaaat aaattaacca atttagtgcg cccgtgttaa aataacttga
      241 caaaattatc tcttctgtgt aaaaacgcca catcgtaaat gaatattcat tcacgcagca
      301 aaacgggtac ggattaatga gtaaaaccga caagcgcgcc gacatcatag aggtggccct
      361 taatctgatt gccgagcgtg ggtttcacga ttcccccatg tccttgatcg caagagaggc
      421 gggagtggcc gccggaacca tttacctgta ttttgaaagc aaggacgacc tgatcctcgc
      481 gctgactcgc aatctggaag acaggatcaa tgaaatgatt caggacggat atcccgtcga
      541 ggattcgatc cgggaaaaat ttctctacat gaacaggaaa atccttaaat atttcatgga
      601 acatcccctt cattttcggt acatggagca gtttttcaac tctccgtacg gggtatctca
      661 ccgcaaagaa agacttttgg acaaatcgga ccgaaacaaa atgatgaccg acattttcga
      721 gcacggcgtc gatgagcagg ttctcaaagc cctgccgatg cccgccctgt ttgccctggc
      781 ttttggtccg attcccacgc tcgtgcgcaa tcatattctt ggatttttct atctggatga
      841 agcgaccatt gaaaaggttg cccaagcgtg ctgggatgca atcaggaagt gattgtcccg
      901 tctggagggc tgccgcggcc aaccgccgag cagcgaacga aagcctgacg aaaagatttt
      961 gttttcggaa aggcgcaatt atgccggaaa agcggcttca tcgaccgctg aaggattcaa
     1021 aattttaaaa taccagaggt gccgaatgca gattcacaca atggccgtac gaatgatcgt
     1081 catcagtagt cttctcgccg caggaatcct cctgaacggc tgctcggata aagggaaggg
     1141 aggccccccg ccgccgcccg aagtctccgt agtgaccatc cagacaaagc cggtccccat
     1201 tacgaccgaa ctgcccgggc gcacttcggc gcatctgatc gcggaagtgc gcccccaggt
     1261 ggacggcatc atccagaaac gcttcttcac ggaagggact aatgtccggg ccggacaggt
     1321 gctttttcaa atcgacgcat cgctttacca ggcggtgttt gataacgcga aagcctctct
     1381 tgcccgctcc gaggcaaatc tgtcgacgat ccagttaaaa gccgcccgtc tgaaggaact
     1441 gctcgccgaa aaagcggtaa gccagcagga ttatgacgac gcagcagccg ccctgaagca
     1501 aaccctggcg gatatccagt tcggaaaggc ttccgtggag gcggcccgga tcaatttaca
     1561 gcacacgacc ataacggcgc ccatctccgg ccgcatcggc cggtccaact tcacggaggg
     1621 cgccctggtg accacgcacc agccccagcc tctggcaacg atccaacagc tggaccccat
     1681 gtatgttgac gtaacacaat cgacggccga aatcctccgc ctgcgtcgcc agcttcagga
     1741 aggccgcttc gaaatcagca aaaaaaatct gggcggcgtc cggctcattt tggaagacaa
     1801 cctggagtat ccctggacag gcacgctgca attccaggat atcacggtcg atccgacaac
     1861 cggatccgta atcctgcgca tggtatttcc caatccgaaa ggcattttgc ttcccggtat
     1921 gtttatccgg accgtcatac aagagggctt taacaagcag gccattttag tcccgcagca
     1981 gggcgtattc cgcgatccca agggcaaccc gttcacgctg gttgtcgcca ggaacggcaa
     2041 aatcgagcaa aaacctctga cgctcgaccg agccatcggc gacgcgtggc tggtgacatc
     2101 ggggctggct cccggcgacc gggtcatcgt cgaggggacg ctcaaagtaa aacccggcat
     2161 gatcgtcaaa gccgtcccct ttggacaggc ctcacagcat gacggcagag cgaaaacacc
     2221 cgctcctccc ggcaaaacca attaacgggg aggacaaaaa atgctgtcaa aattcttttt
     2281 ggatcgtccg gttttcgcgt gggtcatcgc catcattatc atgtcggtcg gcatcctcgc
     2341 catttacaat ctacccgttt cccagtatcc gcaaatggcg cctcccagca ttgccgtcac
     2401 agcgtactat cccggttctt cggccgaaac ggtggaaaac agcgtcacgc aggtcatcga
     2461 acaaaagatg accggcctgg acaatatgat ctatatgtcc gcctccagtg attcagcggg
     2521 gtacacccgt ctggaattga cttttgcgcc gggaaccgat ccggatatgg cctggtccaa
     2581 ggtgcaaaac aaagtccagg ccgccttgac gagtctgccg gacatggtgg tcagccaggg
     2641 cgttgacgtc agaaagtcaa cgcgtaatta cctgatgttt gtcggcctga tttcggaaga
     2701 cggctccatg gacggcatgg acctcagaga ctacgccaag tccaacctgg aaaagattct
     2761 ggcgcgcgtt cccggcgtgg gcgaagtgga agtcttcggc ggcggatacg ccatgcgcgt
     2821 ctggctcaac cccaaaaaac tgacggacta tcatcttacg gtggaagatg tgattggagc
     2881 gctcaaagcc tacaacgtgg aggtttcggc cgggcagttc ggcggcgcgc cctccattcc
     2941 cggccagcgc ttaaatgcca ccatcatcgt ccagaatatg ctttcaacgc cggaggaatt
     3001 tgcctcgatc ccgcttcgga ccaatccgga cggctccatt gtccgcatgg gcgatgtggg
     3061 aagaacagag ttgggcaccg aaatatacga cattcaggtc agcaacaacg gcaggccgtc
     3121 ggcgggtatc gccatccgtc agtcgccgga cgccaatgcg ctggatacgg ccgacgccgt
     3181 gaaggcaaaa atggctgaaa tgagccgctt tttccccgcc ggcatgaaag ttgtttatcc
     3241 gaacgattcc actatattta tcaaagtgtc catcaatgaa gtcatcaaaa cactggtcat
     3301 cgctattttt cttgtttttc tggtgatgtg gcttttcctg ggaaacatgc gcgccacgct
     3361 gattcccacc atcgccgtgc cggtggttat tctgggaacg ttcgccattc tggggctctt
     3421 cggtttttcg attaacatgc tgacgatgtt tgccatggtg ctggccatcg gccttttggt
     3481 cgacgacgcc atcgtcgtcg tcgaaaatgt ggagcgcatt atgagcgagg aggggttgcc
     3541 tccgaaggaa gccacggcta aatcgatgga gcagatcacg agcgcgctcg tcggcatcgg
     3601 cctggtattg tccgccgtgt tcggtcccat ggcctttttc ccgggatcga ccggcgtcat
     3661 ctaccgccag ttctccatta cgatcatttc cgccatgctt ctttcggtga tcgtggcgtt
     3721 gatcctgacg ccggttttgt gcgcagcctt gctcaaaccg gtcaaagccg gtcatgagcc
     3781 ggcggaaaac gccatctttt tcctgaaacc ctttttcatg tggttcgatc gggttttttt
     3841 caaactccgt gatcgttatg tcggcatggt cgatcatacc ctctcgaaga aaaaacgcta
     3901 tctggtcgtt tttctcgtaa tcttggtcgt catggcggtt ttgtttctgc gcatgcccac
     3961 cgcctatttg cctgatgaag atcagggaac cctgttggcc caggtgatca tgccatcggg
     4021 atcgacgctg gaacaaacaa atgaggtcac caaacaaatt gaaaaatact ttcaggaaaa
     4081 tgaaaaagaa gcggtggaga tggtcatggc cattaccggg tacggtttct ccggacgctc
     4141 gcaaaataac ggcatggttt tcatccgtct caaggactgg catctgcgcg accgttccga
     4201 tctgaaagcc gcggcaatcg tcaaaagagc catgggcgcc ctttccacca tccgcaacgc
     4261 catggtcttc accttccagc ctcccgccgt tattgagctg ggaacggcga tcggttttga
     4321 ctttcagctt ctggacaggg gcgcgctggg gcatcaagcc ttgatggacg cccaaaacca
     4381 gcttctcggg ctggtcgccc aggatccgag agtcatgaaa gtccgcccca atagcatggc
     4441 ggatgttgcc gaatatcgca tcgatgtgga ctgggaaaag gccggcgcac tcggcgtgcc
     4501 tattaactcc atccatacga cgctctcggc ggctttcggc agcgcctatg tcaacgactt
     4561 cgtccaggga ggccggataa aaaaagtcta tgttcaggcc gacgcgccct tccggatgct
     4621 gcccaaggat cttgaaaagc tttacgttcg caacacccag ggcaagatgg tgcctttttc
     4681 ttcgttcgcc acgggtcgct ggacatcggg atcgccaaga ctgacacgat ttaacggttt
     4741 cccatccatc aacatctggg gggagcctgc gccgggcaga agctcgggcg aggccatgca
     4801 ggccatggaa gaagcggcgg ccaaacttcc ccagggcttc ggttttgact ggagcggact
     4861 ttcctaccag gaacggatgg ccagttctca agcgccgctt ttgtattcct tttccatttt
     4921 tgtaatcttc ctgtgcctgg ccgcgcttta tgagagctgg gcgattccca tcgccattct
     4981 gctggttttg ccgctgggcg tcatcggcgg aatcatggcg tccagttttc gcggtctggc
     5041 caatgatgtg tacttccaga tcggactgct gaccacgctg gggctcacga caaaaaacgc
     5101 catcctgatc gtccagttcg ccaagggcgg cctggaaaaa ggcatggggc ttttggaagc
     5161 gaccctggag ggcgcgaaac tgcgcttccg ccccatcatg atgacatcgc ttgctttcgg
     5221 tttcggcgtg ctgccgctcg ccacgaccac cggcgcgggc gcgggcgcgc aaaacgccat
     5281 cggcaccagt gtgctgggcg gcatgatcac ggcgactctg ctggcggtga ttttttcacc
     5341 gctgttttat gtgctggttg aaaccttgtt cggcaagaaa ccgaaaacgg acaccggcgg
     5401 gccttctgtt tttaaccgtc tgctggaaaa ggttcttccc aaacgcctga aacagaaaat
     5461 gaccgccgcg gacaaaaccc atgcttcaga ggaacaaccc catgaataaa aaacgacttt
     5521 tgattctgat ggttttgctg gccggctgca ctttggcgcc gaaatatgat cgtccggagg
     5581 cgccggtgcc gaaaacttgg ccgtcaggcc cgtcctatca ggacacgaaa caggcgccgc
     5641 aggcctctca actgccatgg cgggatttca tcaccgatcc ccggctccgg gaagtcatcg
     5701 cagtcgcgct ggccaacaac cgcgatctgc gggtcacggg cctgaatgtg gaaaaagcca
     5761 aagccatgtt cggcatcaac cgggcgtcac ttttaccgat tgtcaacgcg accggaaact
     5821 ggtaccagga acgggtgccg gcggatcttt cctcctcggg aaaaatctac actgcagaga
     5881 agtacagcgt caatctgggc ctcgccgcct gggaaatcga ctttttcggc cgcatccgca
     5941 gtttgaaaga cgccgcgctg gagcaatacc tggccacgga tgaggcaaga cgcagcgcgc
     6001 agattctgct ggtggcgacg gtgacgcagg cctacctggc cctggccgcc gaccgggaag
     6061 cccttcaact ggttgcctca acgctggaag cgcaggaagc ggcctacaac atgattcgga
     6121 aacgccacga agttggaatg gcctccgagc tggatcttcg ccggtcgcaa acccaggtgg
     6181 agacggcccg gggagacgtc gcccgctaca cgcagcttgt cgcgcaggat gaaaacggcc
     6241 tgaatcttct tgtcggatct tccgttcgtc cggatctgct tccccgggat ctgtccggcg
     6301 tgaccctgcc ccgggacatc tccgccggac tgtcctccga cgtgctgctc ggacggccgg
     6361 acgttttggc ggcggaacac ttcctcaaag cggccaatgc caatatcggc gcagcccgcg
     6421 cggctctttt ccccaggatt tcgcttacga cctccttcgg aacggccagc gcagatctgt
     6481 ccggcctgtt caagggaggc tccgatgtgt ggacgttcgc gccgcagatt gtgacgccga
     6541 tttttgatcc tcgtttgtgg tacgcctacg acgtcacgaa aatcgagaag gaaatttcca
     6601 tcgcccagta tgaaaaatcc attcagacag cgttccggga agtggcggat gcgctggccg
     6661 aaaagggaac ggtcagccgt cgcgtgacgg cgcaacaatc cctcgtagac gcgatggccg
     6721 ttgtttaccg tctttccgaa atgcgctatg aaaaaggaat tgacagctac ctctctgttc
     6781 tcgacgccca gcgatcgctt tacggcgccc agcagggcct gatcctgctg cggctggcct
     6841 ccgtcaacaa tatcgtgacc ctttataaaa cgctgggcgg aggagcgtcg tcatgaccgg
     6901 agttccggcc aaggaaatca aatttttctt attgaatcac gttctgacgt ccgtcatctt
     6961 tttcactttg cgactttacg caaaaacgct ccgtgtgcaa atcgagggcg aggagaaaat
     7021 tctggagtat ctggcgcgtg aagggacgat cctctttgcc tgctggcacc agcgcctttt
     7081 cggcggtttt ttcgttccgc ggttttttca atggaagccc tgcatcatga tcagccaaag
     7141 ccgcgacggc gatttgattt ccaaaatcgt atcgcgactc ggctggattc ccgttcgggg
     7201 ctccagcacg cggggcggcg ccagggcgtt tcgcgtcatg gcgcagaacg tcaaaaaaaa
     7261 ccgcatcggc gcccatattg tggacgggcc caccggaccg gcccgcatcg tcaaaccggg
     7321 actgacggcg ctggcccgga gcgcaaacgc agtcatctgc cccgccgtgg tctcctatca
     7381 aagcgcctgg acggcaaaca gctgggaccg ctttctcgtc cccaaaccgt tcacccgggt
     7441 cctgatccgc tttgggtcct tcttcaatgt gcccgatgac gcggaccgaa atttgctgga
     7501 agcgatccgc cttcaggtgg aacagcacat gattgaagcg tacgaagccg cggacagcgg
     7561 ctggggaaaa tgaccgaaac gcgcggcccg gagacggcgc gcacgctccc gcgcgcaaaa
     7621 aacgcgccgc atcccctatc cttttttcag cggcaccggc tgtatccatc ttttatggtc
     7681 gccgtcgtaa taccatttgt cgggaaagcc gcggtggcgg atgaaggtgg gatgttcggc
     7741 ccagttcagc aggtcctgat aggcggcgtg aatcctgcgg aaggcggcgg ccgtcccccc
     7801 ggcgtcggga tgatgaattt tcacctggcg gcggtaggcg ttcttaatga ttttgtgcgg
     7861 ttcacgggaa gcaagatccg ctttggccat atccaaaagc aggatgcttt ccggtttgac
     7921 gccgggaacc ttcacccacc tggggcgctt gagtccctcg gccatcgcca gcctttccgc
     7981 catttccaaa acgcgcctgg aagccagata gtttttggtc gtcctttttt cctcggccca
     8041 ccaggcatcc cccagccggt tgctcatggc gccgaaatct tccgccggct tgacgccggc
     8101 cgtgcggcgc gccttataac ggaatacttc ccggatgccg tgcggaacca cgtccaggac
     8161 aatcagcgta tccgtaaaat agaatgtcgc gtagcgcgta ttcagggcct gcaggaggcc
     8221 gcttcgcgta ttgagtttct ggcggagctg ctggcggcac cggatggagc agtaacggcg
     8281 ctttccgatg tttcccgcgc cgcaggaaag acaaacaccc tggtgggaag aatgagcgga
     8341 agttttgctt ttgaccaaca tgtcgtgctg tgaatcagcc ggttgatcaa aatgatcaac
     8401 cggctttgcc tgtccttcaa ttatttattt ggccgccagg tatcctttgg cgagcgcctt
     8461 attgacgtgc agaacgcctt tgttcatctc tttcatcgga taatcggcgg tgatcacggg
     8521 aagcgcgtcg gcgtcagcct gggttttgac caccgatcca cccggcacat agcgtccgtc
     8581 cgccacccga acgccgatta aaatagcacc cggctcaatg acacagtttt ttccgacaaa
     8641 cgttttgaag accagcgccc tcatccccac aaaggtgtcg tccatcacga cggccggtcc
     8701 gtgaatatgc gcctgatgcg ccagcgacac acggtttccc acgtacaccg cgtatttttt
     8761 tccgtccaca tccacgcggt ttttttccac gggcttgccg tccagctccg tttccagcgc
     8821 gtgaatgatc acgccgtcct gaatattgga atcatctccc acgtggagcg gttggccttc
     8881 gtcgccgcgc acggacgcca tcggcgaaac catgatgttt tttcccagct caacattgcc
     8941 gatgaccgcc gccaggggat gcacaaaggt gccttctcca atcaccggtt cggacacatt
     9001 cgcgcaaaaa tccgttttta cgtttttttc gatcatgtta ccctccgctt cttcgttgta
     9061 tgtctgcgcc gcttcctctg gaggctcaac gacgcgttgc tccgtcagtc ttccgcctgc
     9121 aaagcttttg ccttttccct ggccagcaaa tcctcccaga cggccgccac ttttctttcc
     9181 gtttcttcca tcacgcattg attgtcaatc acaatcgtcg ccagttgtat tttatcgtat
     9241 atcggcatct ggttggccag ccgcaattcg gcgtcctccc ggcagacgga actgcgcgcc
     9301 atcaggcgtt ccacctgttg cccgggttca attttgacca gaatcgtcag gtcaaacaga
     9361 tgctgcattc cgccttcaaa caggagaggc acatcggaaa aaacaatcgc gtgaggagcc
     9421 tgcgccttga ttttttcgag acgggaccac cattcttcaa aaaccctcgg atgaacgatg
     9481 gcgttgagcc tccgcagcct ttccgcagag cggaaaacga tgtccgccat tttgccacgg
     9541 tcgatggtct gatcgggcag gaggatttcg gatccgaagc aatggacgac atcctgccag
     9601 gcgggctttc ccggctcctg cgcttcatgg gccagcttgt caaaatcaat cagacggccg
     9661 cccaggcgca caaacatttc cgcaacggtt gattttccgc aggcaattcc gcccgttaat
     9721 ccgacattca gcatgcgcta cttcttattg cgttcataga taatccgcag accatccagc
     9781 gtcagcattt cttcaatctt gtcgattgtc ccggcttcct gcgcaaccac ccgcgccagc
     9841 ccccccgtgg caatgaccag gggattggtt ttgacttccg ctttcatccg gttgacgatg
     9901 ccgtccacca ggccggcgta gccgaacatg atgcccgcct gaatggcgct gacggtgtcc
     9961 ttggtaataa tggttttcgg cttgccgaat tcgacgcggg gcagcttgga agccttttca
    10021 aaaagcgctt cgctggaaat cacaatcccc ggcgaaatgc agccccccat gtattcgccc
    10081 cggggcgaaa catagtcaaa cgtcgtcgcc gttccaaaat caacaatgat cgattcctgg
    10141 cggtacttgt cgtaggcggc cacggcattg acaatccggt ccgcgccgac ttccttggga
    10201 ttgtcgtaaa aaatcggcat gcctgtttta atgccggggc cgacgatcat cggtatgatg
    10261 ttgaaatact tgacgcagag gggctccaga atattgagca tcggcggcac gacgcaggaa
    10321 ataataatcg cccggacgga tttaatctct ttggctctca tgcgggtgga ctggtacaga
    10381 ttgtaaatca gcacgccgta ttcatcgatg gtatgatcga tttccgtccg gattcgccaa
    10441 tcgtgaagca gctgatcctt gtcaaaaagc cccagcacgg tgtttgtatt gcctacgtca
    10501 atcaccagca gcataaatcg ttacctcttc cctacggtcg cgtctcccgc cgcgaccttc
    10561 accttttggt tgccgtccgc cagaatcacc agcgaaccgt cttcatccag atccgccgcc
    10621 tctccgctga ttgtttcctt cccgaaggtg accgaaaccg gtttgccgat catcgttgtc
    10681 cgctcaagcc attttttcct gagcggcgca aacccgtttt gcgccagctc cctataccat
    10741 tttgccagat tttcatacag gagaatgatc agctccagtc gggaaacttc ccggccggcc
    10801 tccaccgaca gggaggtggc tatttttcga atatcctcgg gaaattcttt gagattccaa
    10861 ttgacgttca cgccgatgcc caccacgaca aaatcgatga cgcccgccgc cgttttcgtc
    10921 tgagtcagaa tgccgcagac cttttttccg ccgatctgca cgtcattggg ccatttcaga
    10981 aaaaccttgc ccgggcaata ggggtcgagc gtttccgcga cggccacgcc cgccgccagg
    11041 gaaatctgcg gcgcgaggtc cggttcaaaa cgcggacgca aaaggacgga cgtgtaaata
    11101 ttggcgcccg gaggcgaatg ccaggcgcgc tgcatcctcc cctttcccgc gctctgacgc
    11161 tccgcgatca acgccgttcc ttccggcgct ccctgcacgc ccagccgaaa cgcctcgtcg
    11221 ttggtcgagc cgatttcttc gtaatagcgc agcgaatggc cgataaattt tccggcaaga
    11281 tgttttttca gggatgcttc gtttaagggc ataccattgc aagttacaag ttttaaaatt
    11341 tatatttcac gtcagggcat ctgctgttgc aacagttgtg ccccaatgcc tgcttcaccc
    11401 ttcggtcttt atcctgttat cttcagcgaa atatcagccg cgcggacgga atgggtgagt
    11461 gcgcccacgg aaatcccatc aacgcccgtt gaggcaattt cgcggacgcg cttcagtgtg
    11521 acgttgccgg acgcctcgat gaaagcgcgg cccgcaaccc agtccacggc ttttttcatg
    11581 gcatggatcg tcatattgtc cagcatgatg atgtcggcgc cgcaaaccag ggcttctttg
    11641 acttcccgca ggttcttggt ttccacctcg attttgaaag atcgcttcca atgttttttc
    11701 cgggcggcaa ccgccgcggc gatgccgccc gccgcttcga tatggttgtc cttgatcagc
    11761 acggcgtcga acaggccgaa gcggtgattg gttccgccgc cttggcgaac cgccatctta
    11821 tccaaagccc tgagccccgg aacggttttt cgcgtatcat aaatttttgc tttcgtcttg
    11881 ccgacggcat ccacgtaagc ggcggtcagg gtcgcaatgc cgcacatgcg ctggaaaaaa
    11941 ttcagcgccg tgcgctcggc ctgcaggatg gcggacaatc ggccttccac ccgcgcgagg
    12001 accgcgcctt tcctcacccg gctcccgtcg gcgaccactt tcctgacgac gatatccgga
    12061 tccagacatt gaaacaccgc ttcaaaaacc tccagacccg ccacaacaaa attctctttg
    12121 gccaccgcaa ccgcccggcc cttctttttc ggactgacgg tggcctgcgt ggtgatgtcg
    12181 cccgtgccga tgtcctcggc cagggcggcc ctgatgattt tttgaattcg ggaatcgatc
    12241 attttcgcct tacatttcaa cccacacaaa cgccggaggg acggctttaa accaccgctg
    12301 cgggcggcaa gaccgttccc tacaggttca tctctttcaa tttctttaac gccccttcca
    12361 gagcggcaaa cttttcctga aaactcttca gtttgccttc ttccttttca atgatctccg
    12421 gcgccgcctt gtcgcggaaa tcatggttgg ccagcttccg cgagctctgc tcgagatctt
    12481 tggacacttt ggccagctct ttttccagac gcgccttttc tccggcaatg tccacgatgc
    12541 cggccagggg aacaaaaatc ttcgtcgaac cgatcacgcc ggtggcaacg cctttgggct
    12601 ccaccagatt ttcttcgacg gtcagggatt caagattggc caggttgacg atgtagcccg
    12661 ctccgttctc cacagaggcc cgggtggccc cgtccgccgc ggaaatcatc actttcagct
    12721 tcacggaggg cggaatgcgc atttcccccc gaatgttacg aacggacgtg atgatgtcca
    12781 tgagcatttc catttctttt tcggcttcct tttcgctcca ttcctcccga acagccggaa
    12841 accggctgac catgatggag ttttcatcgc tcccggagag cgcctgccag agctcttcgg
    12901 tgacgaacgg catgaaggga tgcaggagct gcatcatggc gccgaaaacc tccttcaggg
    12961 tccgctgggt tgccagcttc cgctcggggc tgattttccc gtacagcgcg ggtttgctca
    13021 cttccagata ccagtcacag tattcatgcc agatgaaatg atagatggaa gccgccgcgt
    13081 cgttgaatcg gtattcgtcg aggcttcggg tcacttcctc gatcgtctcg ttgagtttcg
    13141 ccttgatcca tttttccggc agcgcgtcgg gcgcattccg tgagaggttt tcatcgtaat
    13201 ccgccagatt ggaaagcgtc agccgggcgg cgttccagat tttgttgacg aaaaatttgt
    13261 agccttcgat gcgttccggc gacatgcgga catcgcgccc ctgcgcggta taggccgcca
    13321 gcgtaaaacg aaacgcatcc gtgccgtacc ggtcgatcat caccagcggg tcgatgctgt
    13381 ttcccgagga cttgctcatt ttgtcgcctt tttcgtcgcg caccagcgcg tgaagataga
    13441 cgtctttaaa cggcacatcc ttcatgacgt aaaggcccat catcatcatg cgggccaccc
    13501 agaaaaacag aatgtcgaaa ccggtaatga gcagcgaagt cggataaaat gttttgagcg
    13561 cctgtgttcg cctgggccag cccagcgtcg taaacggcca caaagcggag ctgaaccagg
    13621 tatccagaac atcttcgtct tgtttcaatg acgcgccgtc gcagaccgga cacttgtcgg
    13681 gatcgaccgt cgagacaatc accttgccgc agcctccgca ataccagacc ggaatgcggt
    13741 gcccccacca caactgccgc gagatgcacc agtcgcggat attgctcatc cagtcaaaat
    13801 acgtggcctc ccaggaggaa ggcacaatgc gcgttttctt tttcgtaacg gcggcaatgg
    13861 ctttggcggc cagcggtttg attttgacaa accactgttt ggacaccatc ggctcgatgt
    13921 ccgttttgca gcggtagcac tggccgacat tatgcgcgta aggctcggtg ccgaccatat
    13981 agcctccctt ttccagatcg gcgatgacgc tggcgcgcgc ctcgtagcgg tcctgtccct
    14041 tgtattttcc gccgtgttcg ttaatgatcc cctctccgtc gatgaccgag ataatctcca
    14101 gatggtggcg ctgggccatg gcaaagtcat tggggtcgga accgggggtg atcttcacgg
    14161 cgccggagcc gaaatccatc gtcacgtaat cgtcggcgat gattggaatt tccttgttca
    14221 tcaacggcag aatcaccttt ttcccgatct tgtcgcggta gcgcggatca tcgggatgca
    14281 ccgccacggc ggtgtcgccc agcatggtct ccggccgcgt cgtggcgacg acgatttcgc
    14341 ccttgccgtc ggcaaacggg tagcggatgt tccacagatt gctctgatcg gacttgaatt
    14401 ccacttccag gtcggaaatc gcggtgtggc agcgcggaca ccagttgacg atgtagtcgc
    14461 cctgataaat catatcttcg ttgtagagcc ggacaaaaac ctcccggacc gcctgggaga
    14521 ggccctcgtc catggtaaag cgttcacggt cccagtcgca ggagcagccc agccttttga
    14581 gctgattgat gatgacgccg ccgtattttt ccttccattc ccagacgcgg gcaataaatt
    14641 tttcgcggcc gagatcgtgc cgggagagcc cctccttgcg caactgctgc tccaccacgt
    14701 tttgcgtggc gatgcccgcg tggtccattc ccggcatcca cagggtgttg tgtccctgca
    14761 ttctcttgaa gcggatgata atgtcctgga gggtattgtt gagcgcatgg cccatgtgca
    14821 gcattccggt cacattggga ggcggaatga caatggaaaa cgcgggagat tcggatttgt
    14881 cctttgcggc aaaaaactta ttcttcagcc agtagtcgta cagtcttttt tcggcatcgg
    14941 ccggttcaaa cgccttgccc aactctttca tcgccatgga aatattgctc tcctgtctag
    15001 ggatcatcaa aaagggtttg cgcccttttt tcgacggtca ttttccggcc gtccgtcctc
    15061 attaaaattt gaggcgggac aaaccccttc ccgcctcctc aatgtttcaa aaccatcatt
    15121 gttttaatcg aatttcagtt cttccttgat tttgcggatg atcaaaagag cgtccgctcc
    15181 gggcacgggt ttgagcggcg caaattcgcc ggtcgtaata tccgtggctt ccatgatgcc
    15241 ccgggaggtc accaccatga cggcattgaa gtaaggcagg tccgaccgga ggtcgggaaa
    15301 gggcgacgtc gagccgataa acttggtggc cagcgcattg tcgcccgtca ctttgattaa
    15361 aatatcttcg atcatcaggg catactcggc gcggttcaga gcgcccgcag gctcgaaatt
    15421 gccctgcgga ttgatttcca ggccgcgaac accgatggcc agaatccctt cgatatccgc
    15481 tttcaaggga tggttggcaa tgtctttggc cgtcaatgcg gccggcccgg cgctccgggc
    15541 agccttttcg ggatctttaa aggacgtatc aaatgttttc ggtgttctct ttttgtacag
    15601 gacatcgatc ttcaactctt ccatgaacag ggccgcggcg tccgcgcggg aaatgctctc
    15661 cagaagggca atctttttgc ccgtcacggt tccgggcatg gcccgctgaa ttttctggac
    15721 cagattccac tgggcattgg cctggctgac atactctttc cggatttcaa gaactttcgc
    15781 aaacatggcc cccgccttgc cgtaatccaa ggccgtcttg taggccagcc ccatgaaata
    15841 cggagcggag gaggaggacg gatccagctt caccgcctct tcgtaagaac tgacgcactt
    15901 tttcagccag cttttgtcct gctcgcagtc cacgccgatt ttcatgcagg caaccttgct
    15961 gagcgtattg acacggatat aaccgatgtg aacggccagc tgctcgtcgg ctgccttcgc
    16021 cgtgccccac gccttctcca actcaccaaa cgctgcggta taattctgct gataggcttt
    16081 cgccaggccc attccggtat aggctttgga atatttgtcg ttgagctcca gcgccaaggt
    16141 aaactcccgt tcagcctcgg aatatttttc ctgatcgatc agtttcatgc ctgtaaagac
    16201 atgatgttcg ggcgtatcca gcgcgtttga cggctgacgg gccttcgtcc cgcacgcggc
    16261 aatcagcaaa acagccgaaa acaaaatgat cgcgaagagg acctttttca tgttcgcgcg
    16321 gcaaaacata tttcgctcct ttctgtcaaa gggttatttc ctgaaaattc attccttttc
    16381 tttcccaaaa ggctttgtat cattagcttc agtgtctgct gaagtcaagt caatattgcg
    16441 ccagaaattt tcgcgtctgg ccttcaattt tctgtgtctc ttcaggcccg taccacgcga
    16501 tggtcttgtc cgctcgaaac caggtcatct gccgtttggc atactgccag gtctcgtgat
    16561 tgatttcggc aatcgcctga tcccagccgc accgtccctg aatgaatgaa agaacctgcc
    16621 tgtatccgag agactggagg ggtttgagct tttcagagta tccccgatcc atgagccccc
    16681 gcacttcatc gagcagtccc gcttccacca tctgttcggt tctccggtgg attctctttt
    16741 tcaattcatc gcgctccacg caaaggccga tcttcaagac cgtgtaagga caatcggcaa
    16801 accggtgctt tttttgcagc tccaggatgg attgtccgct ttggtccagc acctccagcg
    16861 cacggatgac gcgcacggaa tcgttgggat tgaggcgatc agccgccagc ggatctcttt
    16921 tgcgcagaag cccgaaaaca tacgccctgc cgtaacgatc ccgaagttcc ttgtaatgtc
    16981 ttcggatatt ttcatcgacc ggcggcgtgt cgatgatgcc ctgcaaaagc gcgcggatat
    17041 acaggcctgt tccgccgacc accagcgccg gcctgccctc ccccgccagc ttcgcgatga
    17101 tgcctctcgc ctgctcggaa tacagggccg cgttatattc ttcgtccgga ttcacgacgt
    17161 cgatcagatg atgcctgacc cggtttcttt cctcgcgcgt cggtttggcc gtgccgatgt
    17221 ccagataacg atagacctgc agcgaatccg cattgacgat ctccgcgccc agggattccg
    17281 ccagttgcag cgcgaggcgg gtctttcccg ttgccgtggg cgaaccgatg accaccagcg
    17341 gaatcccggg cgtcttactt tcgtttgaac aatcgttcga tttcatagag ggtaaactgc
    17401 acgcaaatcg gccgtccgtg cggacaggtg gcgttaaaag gggttttttc cagatcccgg
    17461 cacagcgcgg cgacctcccc ggaggaaaga tccgcatggg ctttgaccgc cgcgtggcag
    17521 gccagggacg ccaggatttt ttcctttcgc tccgcaagcg aaggcgccgc ctggccgtcg
    17581 ctcagttgat cgaccaggtc ggatacgagc ccgcggggtt cgacgttcgg cagagcggcg
    17641 ggcatggcct tcaccacgac ggcgtcccgt ccgaagatgt ccagttccag accgatgccg
    17701 gcaaggagcg gcagggcttc ttccaaaagg ctgatctgcg cgggtgtcag cgtcaccact
    17761 tccggcatca acaggggctg gcttgcggcc cgttccattt cggatgcctt caggcgctcc
    17821 agaagaattc gctcatgcgc cgcgtgctga tccagcagca tcaggccgcc ggggcttcca
    17881 aacacaagat acgtgccggc aaactgcccg agatacgttc gatggtcaaa cgtaattctt
    17941 tcttcacagg aggcgtcctc aacgtggctg ggaacgtccg gttccccggc gcgggcgaac
    18001 aaatccccgc caatggcctg ctgcagattc tgccgtgaaa atacgtcttg cggccgatcc
    18061 gatgatgccc ggtaggggct ggatctccag aagcccgaag aagcggatgc tgcctcccgc
    18121 ggcgcgagac gataggcaac cgcatcccgg gaggcctgcg cgccggccag ggcgcgcaag
    18181 accgctccgg acaccaggtc ataaacgccg cgggagtttt tgaagcggac ctccagcttg
    18241 gccggatgca cgttgatgtc cacctcttct ccgggaaggc gaagaaacag aaccgccgcg
    18301 ggaaagcgcc tcggctccat cacctggcgg taggccgcca aaagagcgtg catcatggaa
    18361 ttgtcccgga taaaacggga attgacaaac caaaaaatac tcttggaatt ggacttcgtc
    18421 caggccggcg tggaaacaaa gcccctcagt tctatgtttt ccttgttttc ggcaatcgtc
    18481 aggcgatgat ccgtgaagtc ttttcccaaa atcatggcga cgcggtccgc tacaccggcg
    18541 gcggccggcg ccgcgaacac gtcccgtccg tccgcccgga cggaaaagcg tatttcagga
    18601 tgagccagcg ccaggcgggt gaccgcatcc aggcaggcgc tctgctcggt ggcttctgtc
    18661 ttgagaaact tccggcgcgc cggcacattg gcaaaaagct ccgtcaccgt aatctgcgtg
    18721 ccgggaggaa gacccgccgg agcaatttcg gaaatcgttc cggcctcaac cagagcccgc
    18781 gtcccggcca gatcatccgc gcgacgggtc agaagctcca cgcgggcgac ggaggcaatg
    18841 ctggccatcg cctcaccgcg aaagccgaac gatccggcat cataaatatc atcgatccgg
    18901 acgattttgc tggtggcgtg gcgttcaaaa accagcgcga catcttccct ttcgatgccg
    18961 gatccgttgt ccgaaacccg aatggagcgg catccgcctt tctcaatctc gacgcggatg
    19021 tcgcggcccc cggcatcaat ggcgttttcc accagctcct tgacgatcga cgcaggcctt
    19081 tccaccactt ccccggccgc aatctggttg gccacttctt cggataaaac ggcaattcga
    19141 cgggacaata tttatatctc ctgtgaacac aaaaagccgc agccaccaaa cattcgtgac
    19201 tgcggcccgg ttgattcaat ctttcgcgga cgatttaatc cagcacaatc atgacgcggc
    19261 attttttcat gaaagacgcg ttttccgccg aggcgcgaat gagctgggca tcggcgttgc
    19321 tgatcacgag atcggaccgg cccgcgccgc cggtttttaa tcctttaacc gtgatcgggc
    19381 tggccgtgac ccgctgattg ctctgcgcgg cggtgagatc ccgggcatag ccgctcatcc
    19441 cctgctgaac ggcatactcg cggtccacat tggccgaacc atatacttcc ttgccgtcct
    19501 cgtcgtagat gcgcggcgac atggccggac gcgcctgaat gccgcgggca tcaaccacca
    19561 gtcccgtata ggcaacggca gccggaggcg gagggggcgc gggcgcggcc acaaccggcg
    19621 cgggaggagg agccggcgct tccacaacgg gagggggcgg cggaatttct ttgcgcttct
    19681 gaatggaaat gggaatcatg atcttggata attcgccgta cagcggcatt ctcaacgtca
    19741 cttccacggt gccgtcggac agatactgct gatcaacgat ttgcgcccct ttgaccaggc
    19801 cttcgacctg cgtattgatg acgtcgcttt ccacggcaaa atcctttacc gtcgtccggg
    19861 aatccacctg aacccctttg gtaatttcca gcagattacg ataggcgtcc actttcgcag
    19921 cgcgcagcgc catgggccgg gcattgattt tgcccagata cctgtccggc ggcgcgccga
    19981 tgccttttgc ttcgataaag cccgccgacc agttgatcga accctggccg actttttcca
    20041 cccactcgga catgctgatc gcgttctggc agaaagcggc ggatgtccat accaataaga
    20101 tacccgtcaa aacggttatg gaaacgaatc tcaacttcat attgtcctcc ttacatggtt
    20161 ggcgtccaaa tattttctct tctcaaaaag cgttataaaa ctaacaaaat attctttccg
    20221 ttgcaataag aattcactta ccggcgcggg tgaagaaaac accctcgtcc gggcaagagt
    20281 tatgcttgaa acaaagcggt ttatgtgata ggggagcgct cgtatgaagg cattacacaa
    20341 acagatgaaa ttattcgcgg tttaaccgct atttttttta atggaacagg agcaacaaca
    20401 atatggatta caaaaaaacg ctgaatcttc cccaaaccga tttccccatg aaggcgaatc
    20461 tggccgggcg cgagccggaa atgctgaaaa gatgggaaga gatggacatt taccacaaga
    20521 tccgcgagtg tgccaaaggc aaaaaaccct atatcctgca cgacggccct ccctatgcca
    20581 acggtaacat tcatctggga acggcgctga acaaaatcat caaggatatc gtgatcaagg
    20641 ccaaaaacat gaccggtttc gacggcgtat atgtcccggg ctgggattgc catggactgc
    20701 cgatcgaaca ccaggtggac aaggagctgg gcggaaaaaa ctccggcctg tctcaggtgg
    20761 aaaaacgccg cgcctgccgc gtctatgcgg aaaagtatgt cggcattcag cgagatcaat
    20821 tcaagcgcct cggcgttttc ggcgaatggg gaaaccctta tctgaccatg gcctatccct
    20881 atgaagccgc cacagtccat gagttcggca aactgtttct gaacggcagc gtttataagg
    20941 ggaaaaagcc cgtgtactgg tgcgccacct gcaagacggc gctggcggag gcagaagtgg
    21001 aatatgccga ccatcatacc ccttccattt atgtccggtt tgccttcaca tcggacatcg
    21061 gggccattcg gcccaaactg gcggggaaga aagttttcat ggtgatctgg acgaccacgc
    21121 cctggacgat tcccgccaac ctggccatcg ccgtcaagga cgatttcatc tacgccgccg
    21181 tgaaaatcga cgacgacgtc ctgatcgtag ccaaagacat gctggattat tgcctggacg
    21241 ctttcgggca ccgcggcaaa gcctgtgaga tcgtcgatga attcaagggc gaagtgctgg
    21301 aaggacaaaa gtgcgtccat cccctgattc agagaaacag cctgctgatt ctggctccct
    21361 tcgtcacact ggaggccggc acgggcgccg tgcacatcgc tcccggccac ggccaggaag
    21421 actatgaaat cggcttggaa tatggactag aaaactacgc gccggtggat gacgcgggaa
    21481 aattcacgga agatgtcgaa cattttgccg gtcagttcgt gtttgacgca aacgataacg
    21541 tcattcagaa gctgaaagaa gctggcgcgc ttttgggaca cgttcccatg cagcattcct
    21601 acccgcactg ctggcgctgc aaaaagccca ttatcttccg ttcgaccgag cagtggttta
    21661 tctccatgga gaaaaacgac ctgcgcaaca aggcgctggc ggccatcaac gaggtgcagt
    21721 ggatcccctc ctgggggcgc gaccgcatct acggcatggt ggaaaaccgc ccggactggt
    21781 gcatttcccg ccagcggctg tggggcgttc cgatcacgat tttctactgc gccaaatgca
    21841 aaaacgaact gctgacgcag gacatcctca accacctggt gtcgctggtg gaaaaaaacg
    21901 gcgcggatgt ctggtttgaa aaagagccga aagacctgat gcccaaacac accgtctgtc
    21961 ctcactgccg gtcaacggag ttcaccaagg aggtcaatat tctggatgtc tggttcgatt
    22021 cgggcgtcag ccacgcggcg gtcctggaaa aaaggccgga gctgggctct ccctgcgatc
    22081 tgtacctgga aggctccgat cagcatcggg gctggttcca ctcttccctt ctggaatccg
    22141 tcggaacccg cggacgggcc ccctacaaaa gcgtcctgac ccacggcttt gtcgtggacg
    22201 gggaagggaa aaagatgtcc aaatccggcg gcaacgccat cggcgcggaa gacgccgttt
    22261 ccaaatacgg cgcggaaatc ctgcggctgt gggtggcggc ggaggactat accgacgaca
    22321 tccgcatttc cgaggaaatt ctcaagcgcc tgatggaagc ctaccggcgc atccgcaaca
    22381 ccagccggtt catcctgggc aatctgtatg actttgcacc ggatgccgac cgtctggatg
    22441 gcgaccaaat gctggaaatg gaccggtggg ccctgcaccg cctgcaggaa gtgatcaggc
    22501 gcgtgacgga ggcctatgaa cgccaccagt tccacatggt tttctatacg ctctacaatt
    22561 actgcacggt ggatttgagc gcgctgtacc tggacgtttt gaaggaccgc ctctatacca
    22621 gcaaaaccgc ctccgccgcc cggcgttcgg gccagacggc gatgttcatc attttaaacg
    22681 cgatgacccg cctgctcgct ccgatcctta cgttcaccgc ggaagaggtc tgggcggccc
    22741 tgcccgcctg gaacggcaaa gaagaaagcg tccatctgac gcagtttccg caggtggaag
    22801 aaaagtattt caacgcttct ctgggcgagc gctggaaagc catgattgac gccaaaagcg
    22861 aaatcgccaa agccgtcgaa caggcgcgca aggaaaaagt gatcggccat tcgctggatg
    22921 c
//
LOCUS       DAOSRM010000024        22796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2442_length_22796_cov_88.852601, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000024 DAOSRM010000000
VERSION     DAOSRM010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 22796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22796
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2442_length_22796_cov_88.852601"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLA58_03905"
     CDS             <1..269
                     /locus_tag="PLA58_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOR61971.1"
                     /translation="ASAVEIDLFKEDESKNLIRISATILVEKESQKAIMIGKKGALLK
                     KIGTRARLAMENLFGAKVFLELFVRVKKDWTTSDKMLRELGLMK"
     gene            279..1598
                     /gene="der"
                     /locus_tag="PLA58_03910"
     CDS             279..1598
                     /gene="der"
                     /locus_tag="PLA58_03910"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOR61972.1"
                     /translation="MQAKPVIAIVGRPNVGKSTLFNRIAGKQKAIVIDEPGATRDRNY
                     MDCVWHDKAFTLIDTGGFEPTTEERILVQMREQTNLAIEEADVIIFLMDGRDGLTPSD
                     TEIARQLRGRGKKVFYAANKVDGARHEELLTEFYRLGIDELHSISAQHGLGVDELLAV
                     LTDDFPEASESGEDENEPVAVAIVGKPNVGKSSLVNLISGRPRSIANPTPGTTRDAVD
                     TVIKVHGRRYLLIDTAGIRRKNKISLTLEKYSVIQALKSINRCEIALVLIDAEEGMTE
                     QDAKIVGLAYERGKACIIVVNKWDKIEKDNATAGKFVEEIHDKIKFMDFAPIVFISAL
                     TGQRAPKIFDLINEVDSQYTKRVDTSPLNDLVKRSLRKNPMPRFQNKPMSISYATQTG
                     VKPPTFVLFVSHARGVHFSYERYLMNAIRSEFGFDKVPIRLIFRKKR"
     gene            complement(2065..3468)
                     /locus_tag="PLA58_03915"
     CDS             complement(2065..3468)
                     /locus_tag="PLA58_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOR61973.1"
                     /translation="MRDFTEGKIARQVIDFTLPIMLGNVLMSAYGIINLIWVGRLLGH
                     KAVAAVAACLPIIMLMPAFLIGLGMATNVLIAQAFGRKDTAMLRKILSNSFFSSILFC
                     LLISLLGILFRHQLLQWVHTPAEIYDMALSYLTIMMAMLVFHFYVSWMNGMLRGLGDA
                     TTVVKILTLLAGFNVVLVPLFIRGIGPLPPLGVAGAAWGSGAAAALTCLIGYVYLLRF
                     NPYINMRQWDYSLDGNIIREVFVIGVPASLQMMVISLAGVFIVSLVNRYGTAVTAAFG
                     IGMQVDMLMTIPFISIGIAATTISGQNLGAGNLERVFETTKISVCFGLVVGLVCTVVL
                     LAFSEQIGGIFLKKSAESSRVLGYVRDYYRWIAFIFPCFGVVFAVHGVLRSAGDTLAL
                     LALSFIAMFMIRIPLAYGLAGPGGFQQDGIWMAMLASSALAVGLNWLYYRRGPWKKKR
                     LLNPSSSPSTKKTSFSA"
     gene            complement(3479..4321)
                     /locus_tag="PLA58_03920"
     CDS             complement(3479..4321)
                     /locus_tag="PLA58_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61974.1"
                     /translation="MSVVPADAATVMLLRPCPGEGAEGIEVLLVLRNRKSSFVPGYYV
                     YPGGVIDAEDYGPGMERFVRGLDRHKAALLIGDMPQAEKALGVWVAAVRETFEEAGLL
                     LARRQDGSPLVMATQEERNRFGRCRQSLVKKETTFSSILETEKLVLFSEDLCYFSHWI
                     TPEPLPRRYDVRFFMAALPVGQSVSHDGVELTSHVWIRPSEALEQYEAGKIGMVLPQI
                     MTLGELSRFRTVEEALQCAKKKRVEATRTKMMYRDGRYVEVMPDGEVFQHRPPVYSWP
                     DEKD"
     gene            complement(4395..5060)
                     /locus_tag="PLA58_03925"
     CDS             complement(4395..5060)
                     /locus_tag="PLA58_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR61975.1"
                     /translation="MELTAAIRGRRSIRKFEPRDIPKEIITDILETARWSPSWGNTQP
                     WSLYVLTGRTLQKFKEMNLLQSMAGAPVASDVPMLEKWPDAMKARYGQLGKVVLSAQG
                     IARDDKAGREKYYASMVTAFDAPCLILACISRDNLVEYQMLDIGLIAQSICLAAYDKG
                     LGTCLLAAAARFPAEIRKITAIPDDKKIIVGIAMGYPDLSFALNQFERERAKLDEFVF
                     WLD"
     gene            complement(5148..5849)
                     /gene="radC"
                     /locus_tag="PLA58_03930"
     CDS             complement(5148..5849)
                     /gene="radC"
                     /locus_tag="PLA58_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOR61976.1"
                     /translation="MSEKLQSSGIKDWPEDDRPREKLLGKGARELSNSELLAILLRTG
                     AQGQSAIDLARKILDKFGTFRNMSQTDTRDWQGFKGLGPAKIAQIHAALEIGRRFREV
                     EVRPEQRKIVSARDVVEIVMPQMRDLKTEVFKVAHLNSNHRLIVIRNAAVGTVNQAMP
                     IVREIIHDALQNFAAAIICIHNHPSANLSPSPEDKKFTGELCAAGKLMGVKVLDHIII
                     GDGAYFSFLDEGLMG"
     gene            complement(5908..6159)
                     /locus_tag="PLA58_03935"
     CDS             complement(5908..6159)
                     /locus_tag="PLA58_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61977.1"
                     /translation="MRQARRFGDKTLSSAKKGGVWQSKTWRQALETLPSAVMGLVSLG
                     LKKGDPVGIASRTRREWSEADPEGIWRAVRPEDPAPGDY"
     gene            6372..7508
                     /locus_tag="PLA58_03940"
     CDS             6372..7508
                     /locus_tag="PLA58_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR61978.1"
                     /translation="MSNNDDGIYRQLQIQLDRYPIGYPATKSGVEIDLLKYFFTPLEA
                     KIALCLTLRSISLTPIRKRLKGKFFIDLSPKELSALLDEMFQKGVIRRSENGGSFRYG
                     IAMLAIGMFEYHVDDLTPELVKMMHQYFGEAFGAEFFRSSLPQLRTSPHLKTLIPEYL
                     VEPYDNMRRFIQHTRETISVANCVCKQGEALLGKPCTQTDTYEVCLLIGSTGYAARGR
                     AREISKDECLKILEMAEEKGMVLQPGNSRKPACICICCGCCCGVLTTAKKQPRPARFF
                     ATNYYARIDAESCTGCGVCVKRCQMEAIVADGQTFRVDRDRCIGCGLCATRCKPRAAR
                     LIRKDKITVPPMNTEMLYLSILMERAGRKKMIVNMLKLLFGKPL"
     gene            complement(7512..8552)
                     /locus_tag="PLA58_03945"
     CDS             complement(7512..8552)
                     /locus_tag="PLA58_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOR61979.1"
                     /translation="MTANLESISVTLVSVLSAFEAGMRKLQPLMIPSIRGELAVHEPM
                     LAAVRASLGTEDGAAGSEDGRRELLRACDFLLRAVRNFGRADDLQDAFISALRASRKI
                     CRAEEALFPLCRLFPAVHRYFLETGADVLPEATIGDSGIGLFHVGANRDLRARGGYSI
                     YIPSTPSSTAARPLVVALHGGYGHGRDFIWTWIREARSRGFVILAPTSEAMTWSIGHV
                     ETDAQMLMRHLEEVCTRVPVDRNRILLTGMSDGGTFALALGLTRDNIFSKIAAVSCAL
                     PPVKISRAGEKHVLWVHGAQDWIFPVGRTVQACRYLQQSGVDIKLKVIPDLSHAYPRE
                     ANDMILKWFGTF"
     gene            complement(8549..9847)
                     /locus_tag="PLA58_03950"
     CDS             complement(8549..9847)
                     /locus_tag="PLA58_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR61980.1"
                     /translation="MPDAVIIGAGYAGMSAAALLSHAGRKVVVLEESERVGGRARSFR
                     DEEGYLREYGAHSHRLAHKGIANEVFKRLGEEIDFLPESKDGKLIFRGRLWERPEGPI
                     GFLKTPLLSIRARLVLLALFAKIKKTDPADWYHRTLLDFYRASFRNPEVEAFLPFLGM
                     TIMCPDPNKVSAGEVIDFLKRVLAAGVGVGEPRGGSAGIFQTLSRHIDRGGEIHLREK
                     AEKIIIENRRVTGVKTGRAIYPAPVVLFAAGLPLIADLLDQTLLPAAVEKYAKNIEHS
                     SGLVIDFITNRPITGIRSGILGVDVPIWARFQSSADPSFTPAGKYLSTWGIMLPWHFG
                     GDASVVAGAEQKMKSVISAVFPDFLPAVVDERKMVATVMNGNVLTPAQSKPNRPDVEC
                     PGIEGLYFIGDTVRGDGCSGDISFSSAMKAADKILSDRKP"
     gene            10037..10537
                     /locus_tag="PLA58_03955"
     CDS             10037..10537
                     /locus_tag="PLA58_03955"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR61981.1"
                     /translation="MSERKRFLGWENPGKALITGASAGIGLSFAQQLAQRGFDLILLA
                     RRRERLETAARKLESDCAIHCEIVTADLSDMDQIKQTADLLQTVDGLDVLINNAGFAT
                     IGPFAGVPLEKSMRMLHLHTTACVMLKIFIPGWKNRFYVWILQHSAAARRIVQNSVKK
                     RSQKKR"
     gene            complement(10554..11741)
                     /locus_tag="PLA58_03960"
     CDS             complement(10554..11741)
                     /locus_tag="PLA58_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR61982.1"
                     /translation="MKKKDESESIFTKSIHAGEDRARYADSITTPIVQSSTYSFKDSR
                     EIEAYTKNDKKRYEYGRYGNPTEKVAERRLAELDGAQEGLMFSSGMMAITTTILALVQ
                     TGDHIVITDDCYGKTLEFCRSYLKRFGIECTIVPFGDYDVLDAAVKKNTRFIFSESPT
                     NPYLNVFDMVKFKQIGDKHKVITIIDSTFATPFNQRPLDYGIDLVIHSATKYLAGHND
                     ILAGVLLGKRELVGKVRELHKSMGGVIDPHCCYLLLRGLKTFPLRVKTQNDNALAIAE
                     YLERHPKIEKAYYPFLESHAHYAIAREQMKGGGGVVTFTIKGDLDAAKRFMDSLGMIY
                     IAPSLGGVETLITHPATVTYYRYTREQRYDLGIVDNLFRLAVGIEDVKDIIADLERGF
                     AGI"
     gene            11926..12174
                     /locus_tag="PLA58_03965"
     CDS             11926..12174
                     /locus_tag="PLA58_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOR61983.1"
                     /translation="MPIYEFECDKCKKQFEVVTLSLSEKPKAVCPKCKSKKAHKLVSK
                     VGKGKYGSLRSGGSLDTGAPSSSGCSSCSSTNCSSCGH"
     gene            12521..12727
                     /locus_tag="PLA58_03970"
     CDS             12521..12727
                     /locus_tag="PLA58_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOR61984.1"
                     /translation="MSSIYKVIEIIGGSEKSWEDAAKKAVEIAAKSLKEIRVAEVKEL
                     DMRIEEGKVVEYRVKLRVSFKYTP"
     gene            complement(12853..13755)
                     /locus_tag="PLA58_03975"
     CDS             complement(12853..13755)
                     /locus_tag="PLA58_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOR61985.1"
                     /translation="MSHNHHHNDQPPSLANLILAILINGGIVVFEMIFGLLISSMALI
                     SDAVHNLSDIAAMSFTCWAEKMSRRPATEHKTYGWRKMEFIAAFVNSIVLSVVIAFVF
                     WESVQRLFSPAQVSGTMMLWVAVVAFVGNSAATLLLQKISARNFNIKSAWLHSLQDAL
                     FSLGVIVGALLIMHFDLYFIDPLISMVICIFIAREIYKIIRHTVNALLDSVPADVDFN
                     AVKRDLLSVSDVAEVNDLHIWQTGTGQKLLSAHLVSDDEAPDHEQIIRAVQKILHEDH
                     GIDHTTIQLLPSSAREMEHCNHCN"
     gene            complement(13790..14962)
                     /locus_tag="PLA58_03980"
     CDS             complement(13790..14962)
                     /locus_tag="PLA58_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOR61986.1"
                     /translation="MKEWQKLAWIAVIFLGAYFIPWESLVVRQAVLEAFMMLQDYARQ
                     HVLTCLIPAFFIAGAISVFVSQGAVLKYFGASANKILSYSVASVSGSVLAVCSCTVLP
                     LFAGIYTRGAGIGPATAFLYSGPAINVLAIILTAKVLGWQLGMARAVGAVAFSIITGL
                     LMAFIFRRDDANRETGEMFLPEEEGKGRALWQDAVYMLVMVLILVFASFAGPTENDGG
                     LWRMIFSVKWFLVMGLLVVLGIMLKKWFVKQELQGWVQATWDFAKQILPLLFGGVLVA
                     GFLLGRPGYPALIPEQYIQALVGGNSLWANFFAAVVGAFMYFATLTEVPILQGLIGSG
                     MGQGPALALLLAGPALSLPSMIAISSIMGIRKTVVYCSIVVLLSTVAGMAYGWLMV"
     gene            complement(14975..16024)
                     /gene="arsB"
                     /locus_tag="PLA58_03985"
     CDS             complement(14975..16024)
                     /gene="arsB"
                     /locus_tag="PLA58_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOR61987.1"
                     /translation="MSEPIADKLSFLDRYLTVWIFAAMILGVGLGYFIPGVEAFINSF
                     QVGTTNVPIAIGLILMMYPPFAKVKYEEMPQVFRNGKVLGLSLAQNWLIGPVLMFILA
                     IVFLPDKPEYMVGLIMIGLARCIAMVIVWNELAKGSTEYAAGLVAFNSIFQVLFYSVF
                     AWFFITVLPPYFGFSGSVVDVSIRQIAESVFIYLGIPCILGALTRLIGVKLVGKKRYH
                     ERFVPVISPLTLIALLFTIVVMFSLKGNLIISLPFDVVRIAIPLLIYFVVMFLVSFYL
                     GRKMGADYSKTTTLAFTAASNNFELAIAVAIAVFGINSGAAFAAVIGPLVEVPVMIGL
                     VTVALHFQQKYFRVP"
     gene            complement(16079..16312)
                     /locus_tag="PLA58_03990"
     CDS             complement(16079..16312)
                     /locus_tag="PLA58_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOR61988.1"
                     /translation="MKIEILGTGCAKCLKLEELVNEVVKESAVNAEISKVKDIKKIMT
                     YGVMTTPGLVVDGQVKIAGKMPTEEQIRGWIVK"
     gene            complement(16322..16654)
                     /locus_tag="PLA58_03995"
     CDS             complement(16322..16654)
                     /locus_tag="PLA58_03995"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOR61989.1"
                     /translation="MKESLRPRYEARAKILKAMAHPSRLLMIEELQKQERCVNELTEM
                     IGTDTSTVSKHLSVLKNAGLVADDRRANCIYYRLRCPCILNFMGCVEEVLSANAEDHK
                     KMMKSCKK"
     gene            complement(16818..17717)
                     /locus_tag="PLA58_04000"
     CDS             complement(16818..17717)
                     /locus_tag="PLA58_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOR61990.1"
                     /translation="MNTLRKAGIFLAVGALLLCACVVFAQEGRVGMSDWTKIIESKVT
                     NQIGDKGLINYEDGYIESVGTGAPPERYYGKPQARPMALRAAQVDAFRNLLETVQGVQ
                     IDSQTTVKDFAVESDVINTAVSGMVKGAQIVKKEYLSDGTVEVTVRMPLSGVARAILP
                     KAIADDKKEDREHKPAPFSKKPAPKDEEYTGLVIDGRGLQARPAMSPKIFDENGSEVY
                     GTLIVNKTYAMQQGICGYARDLTSAQSNARVTNSPLTVKAIRAQGAGMSEFVISNEDA
                     GKIRSAKQNLAFLQKCRVMVVLD"
     gene            18007..18681
                     /locus_tag="PLA58_04005"
     CDS             18007..18681
                     /locus_tag="PLA58_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOR61991.1"
                     /translation="MLALAALLSLAACTARTTVADRLAPYAQATGMENAGQLLLVTDE
                     SFLFLTSHKIYPLEKTNALWQQAMAPMDAVIGRNGFAPPGEKREGDGRTPSGLFRLKT
                     AFGYPPSAPTQMPYRQMLEDDLWIDDPNDPDYNRLIRQNQTKAASYEKMKRDDDLYKY
                     GIIIEYNTDPVIKGLGSAIFLHVWAGANSTTAGCVAASEDDILKILGWLNPAKNPVIL
                     INPDEP"
     gene            18699..19466
                     /locus_tag="PLA58_04010"
     CDS             18699..19466
                     /locus_tag="PLA58_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOR61992.1"
                     /translation="MKLKLFLITLLAAILFSAGEPPASFCEEVMPDTFVHIQEIIPDA
                     LLDIRYFTEHNFLGVKVDGYLAPTCILTRPAAEALARVQEDLAPFGMTIKIYDCYRPQ
                     KAVDHFVRWAKDINDTRTRKEFYPTVDKRHLFRDGYIAERSGHSRGSTVDLTLVPLPA
                     PIQPSYKAGDPLKECYLPAGVRFADNSLDMGTGFDCFGEMSHPENKDIGPQQRANRLL
                     LKTLMARHGFRNLPEEWWHFTLNNEPYPDTYFNFPVK"
     gene            19469..20698
                     /locus_tag="PLA58_04015"
     CDS             19469..20698
                     /locus_tag="PLA58_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOR61993.1"
                     /translation="MKINLPPFLRSYGFSILLVASVFVGALLGILYQKDAARLKPLGD
                     LFLNLLFTVIVPLVFFSIASTVASMTHLGRLGKILLVMILVFVITGLIASAVMMVAVT
                     FFSPASGVSIPLPTNADVQHLKAGDQIVRAFSVPDFRDLLSKNNMLALIIFSMLVGVA
                     ASAAGEKGKDFSRFLTSANSVMMKLISFIMYYAPIGLCAYFACLVGVFGPELLGSYAR
                     TVVVYYPTALLYFFIAFTFYAYLAGRGRGVRVFWSNIIPPSVMSLATGSSMATIPASL
                     QAAEKIGVPKDIREVVIPIGATMHMEGSCLAAVLKIAFLFGIFSMPLTGVDTILTALG
                     IALLTGVVVSGIPGGGTIGELLILSLYGFPPEAFPLVTMIGTLVDAPATMVNAVGDNV
                     SGMMVARVLGGKEWMDG"
     gene            complement(20744..20992)
                     /locus_tag="PLA58_04020"
     CDS             complement(20744..20992)
                     /locus_tag="PLA58_04020"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOR61994.1"
                     /translation="MNFKIVLLIVIACLAVVFVIQNVSAVTVSIFFWEISLSLALLIF
                     FVLAFGFVFGWFLHSFLAYQKVKKEVSEIQNDLRSQKR"
     gene            complement(21279..22670)
                     /locus_tag="PLA58_04025"
     CDS             complement(21279..22670)
                     /locus_tag="PLA58_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61995.1"
                     /translation="MKRFWLVMLSLGLIIAFSSTAMAVDVKFSGEFYAGGMYLDKTNL
                     TKSTTGGDPSTAFYFQRLRARTDFTISPGLTLITRLDAMERAWGATRSATGTALAVDS
                     AGTVAENENIAIDWVYIDYKSAIGTFLVGYMNYGSTGTIFGNSSAPQARIRFNRMFGP
                     INVWASISKVKENSKTAINPVPPATAAFADSDNDVYHLEGIYSWKSGRAGLNVNYYNY
                     ANTRPPAVPAANGSYRRTYFLFTPYLMAKFGPVSVQAEFNYLNGTDKYEDGVVKVAGV
                     DKVTLENISAYLDATANFGMFYAGGSIAYVSGDDPDSKDKTEGGLINGGRDWNPCLIM
                     FSYYDRAKWAGNLTGYGTSVDNGQMANAWFFQLRGGVKPVEKLDVMASVSYAFADKKP
                     WNTANNPATEYLNDDYGWEVDLTATYKITNNLSYMVGAGYLFTGKYYQANVAVNNELT
                     DDFLVINKLTLTF"
BASE COUNT         5384 a         6510 c         5883 g         5019 t
ORIGIN      
        1 atgcgtcggc cgtcgaaatc gacttattca aggaggacga aagcaaaaat ctgatccgca
       61 tcagcgcgac gatcctggtg gaaaaagaat cgcaaaaagc catcatgatc ggcaaaaaag
      121 gcgccctgct caaaaaaatc ggaacgcggg cgaggcttgc catggaaaat ctcttcggcg
      181 cgaaagtctt tctggaattg tttgtccgcg tcaaaaaaga ctggaccact tcggacaaaa
      241 tgctgcgcga gctgggactg atgaaataga ggatcgaaat gcaggcaaaa cccgtcatcg
      301 ccattgtcgg ccggcccaac gtgggcaaat cgacgctctt caaccgaatt gcgggaaagc
      361 agaaggccat cgtgatcgac gaaccgggcg cgacgcgcga ccgcaattac atggactgcg
      421 tgtggcacga caaggccttc accctgatcg acacgggcgg attcgagccg acgacggaag
      481 aacggattct cgtgcagatg cgcgagcaga ccaacctggc cattgaggaa gcggatgtta
      541 tcatctttct gatggacggg cgggacggcc tgacgccctc ggatacggaa atcgcgcggc
      601 agcttcgcgg acgcggaaaa aaagtatttt acgccgccaa caaggtggac ggcgcgcgcc
      661 atgaagagct gctgacggaa ttttaccgtc tgggcatcga cgaacttcat tccatctccg
      721 cccagcacgg attgggcgtg gatgaactgc tggccgtgct gaccgacgac tttcccgaag
      781 cttctgaaag cggggaggat gaaaatgagc ccgtcgccgt ggcgattgtc ggcaaaccca
      841 acgtcggcaa atcctcgctg gtcaacctga tttccggcag gccaagaagc atcgccaatc
      901 ccacacccgg cacgacgcgc gacgcggtcg ataccgtcat caaagtccac ggccggcgct
      961 atctgctcat cgacactgcg ggcatccgcc ggaaaaacaa gatcagcctg acgctggaaa
     1021 aatacagcgt catccaggcg ctcaaatcca tcaaccgctg cgaaatcgcg ctggtcctca
     1081 tcgatgcgga agaaggcatg accgagcagg acgccaaaat cgtggggctg gcctacgaac
     1141 gcggcaaagc ctgcatcatc gttgtgaaca aatgggacaa aatcgaaaaa gacaacgcca
     1201 cggcgggtaa atttgtcgag gaaattcacg acaaaatcaa gttcatggat tttgcgccca
     1261 tcgtgttcat ttccgcgctg acgggacagc gggcgccgaa gatctttgac ctcatcaacg
     1321 aggttgacag ccagtacacc aagcgggtgg acacctctcc tttgaacgac ctggtgaaaa
     1381 gatcccttcg gaaaaacccg atgccccgct ttcaaaacaa acccatgagc atctcctacg
     1441 cgacgcaaac cggcgtaaag ccgcccacgt tcgtcctttt cgtttcacac gccaggggcg
     1501 ttcacttttc ctacgagcgt tacctgatga acgccatccg ctccgagttc ggcttcgaca
     1561 aagtcccgat caggctgatc ttccgcaaaa agcggtaggg cctcgctttt ccatcctgtc
     1621 cgccggcctt tacgcctgat cccgcctttt taaaacctcc ggaagcgcgt agcacgcggt
     1681 ttcttcttgt cgaatttccc cacacggatc cgccaggaaa acaatttcct gggaatccgc
     1741 cattgggcaa catgtccttc acataagcct gaatatcttc cgtggcaacg gtgtttgccg
     1801 attcaggccg gcgcacagca ccgtccattt tccggcgttt cgcaataacg gcacaaaagc
     1861 aatgttgacg gcctcgccaa aagcccggat actgcgttgc gcttcatcct tcgtcattgc
     1921 ggcgcacgct gaagcgcgcc gcattccccg ggatttgcgc gccttgcctg cggagctttt
     1981 tgcgaagccg tctgaatttt ccgttttttc gactttctac gaggtcgtca ataattgaac
     2041 gtttcgtatc ttagactgcg ctgcttatgc tgaaaatgat gttttctttg tcgatggcga
     2101 tgaggacggg ttcagcagac gttttttctt ccaggggccc ctccggtagt acagccagtt
     2161 caggccgacg gccagcgcgc tgcttgccag catggccatc cagatgccgt cctgctgaaa
     2221 tccgccggga ccggccaggc cataggccag cggaatgcgg atcataaaca tggcaatgaa
     2281 ggaaagcgcc agcagcgcca gcgtgtcccc cgcgctgcgc agcacgccat gaacggcaaa
     2341 aacaacgcca aagcagggga agatgaacgc gatccaccgg taataatccc tgacgtaacc
     2401 cagcacgcgg gaactttcag ccgatttctt caggaagatg ccgccgatct gctcggaaaa
     2461 agccagcaaa acgaccgtgc agacaagccc gacgaccaga ccgaaacata cggagatctt
     2521 cgtcgtttca aaaacgcgct ccaggtttcc cgcgcccaga ttctgtcccg aaatggtggt
     2581 tgcggcgatt ccgatcgaaa taaaggggat cgtcatcagc atgtccacct gcatgccgat
     2641 gccgaaagcg gcggtcacag ccgtcccgta gcggttgacc agggacacga tgaagacgcc
     2701 ggccagcgaa atcaccatca tctgcagcga cgcgggcacg ccgatgacaa aaacctctcg
     2761 gataatgttc ccgtcaaggc tgtagtccca ctgacgcatg ttgatgtaag ggttgaagcg
     2821 cagcaggtaa acgtaaccga tcaaacaggt caacgcagcc gccgctcccg aaccccaggc
     2881 ggcgccggcc acgccgagcg gcggcaacgg gccgatgccg cgaatgaaca gcgggacaag
     2941 aacaacattg aaccccgcaa gcagcgtcag gattttcacc acggtggtgg cgtcgcccag
     3001 gccgcgcagc attccattca tccagctgac atagaaatga aagaccagca tcgccatcat
     3061 aatcgtcaga tacgaaagcg ccatgtcgta aatctcggcg ggcgtatgca cccactgcag
     3121 cagttgatgc cggaacaaaa tgcctaaaag ggaaatcagc aggcagaaca aaatggatga
     3181 gaaaaaggag ttggacagga tcttccggag catcgccgta tctttgcgtc cgaaggcctg
     3241 ggcgatgaga acattggtgg ccatacccag gccgatcaga aacgcgggca tcagcatgat
     3301 gatcggcagg caggcggcga cggcggccac cgccttgtgc ccgaggagcc tgcccaccca
     3361 gatcagattg atgatgccgt aggcggacat gagcacgttg cccagcatga tcggcagggt
     3421 gaaatcgatg acctgtctgg caatcttgcc ttccgtgaag tcccgcatga gtttcttgtc
     3481 aatccttttc gtcaggccag gaatacacgg gcggacggtg ttgaaagacc tcgccgtccg
     3541 gcatgacctc cacgtaccgg ccgtcccgat acatcatttt cgtccgcgta gcctcgacgc
     3601 gttttttctt cgcgcattgg agcgcttcct cgaccgtccg gaatcggctc aattccccga
     3661 gcgtcatgat ctgtggcaaa accattccga ttttgcccgc ctcgtactgc tccagggcct
     3721 ccgaaggccg gatccaaacg tggctggtca gctccacacc gtcgtgggac acggattgcc
     3781 cgaccggaag cgccgccatg aaaaaacgca cgtcatagcg cctgggaagc ggttccggcg
     3841 tgatccagtg cgaaaagtag cagaggtctt cactgaagag aaccagtttt tccgtctcca
     3901 gaatggagga aaaggttgtt tccttcttga cgagcgactg ccggcagcgt ccgaacctgt
     3961 ttcgttcctc ctgtgtcgcc atgacaaggg gagaaccgtc ctgtcgccgg gccaggagca
     4021 atcccgcttc ttcaaaggtt tcgcgtacgg cagccaccca gacgcccagc gccttttcgg
     4081 cctgaggcat atccccgatg agcaatgcgg ctttgtgcct gtccagaccc cggacaaagc
     4141 gctccattcc cggcccgtaa tcttcagcgt caatcacgcc tccgggatag acataatagc
     4201 ccggcacaaa actgcttttg cgattgcgca gcaccaaaag aacttcgatg ccttccgctc
     4261 cttcgccggg acagggcctc aataacatca ccgtcgcggc atccgcggga accacgctca
     4321 tgccggatca tccttcctta tgaatttgga taacaggggc ttgttcggcg tcgcctcttt
     4381 cgcggggagc ccgatcaatc cagccagaag acaaattcat ccagctttgc tctttcccgt
     4441 tcgaattgat tcagcgcaaa agacaggtcc gggtagccca tcgcaatccc gacaataatt
     4501 tttttgtcgt cgggaatggc cgtgattttt cgaatttcgg cgggaaaccg ggcggcggcg
     4561 gccaaaaggc aggtgcccag ccctttgtcg taggccgcca gacagatgga ctgggcaatg
     4621 agaccgatgt ccagcatctg atattcgacc agattatcgc gtgaaatgca ggccagaatc
     4681 aggcacggcg cgtcaaaagc ggtcaccatg ctcgcgtaat atttttcccg ccccgccttg
     4741 tcgtctctcg cgattccctg cgcgcttaaa acaacctttc ccagctgccc gtagcgtgct
     4801 ttcatggcgt cgggccactt ctccagcatg ggcacatccg acgcgacggg cgcgccggcc
     4861 atactttgca gcagattcat ttccttgaat ttctgcagcg ttctgcccgt cagcacatac
     4921 aaagaccagg gctgggtgtt tccccaggag ggggaccagc gggccgtctc cagaatatcc
     4981 gtaatgattt ccttcggaat gtcccgaggt tcaaatttgc gaatgcttct tcttccccga
     5041 atggcagcag tcagttccat ggcaacctcc ttgtgtgata tgccgttcca tacaacatcc
     5101 agcgaaataa gaaaagccgg gatgtgcctt ttttttcatt ctgttggtca gcccatcagc
     5161 ccctcatcca gaaagctgaa gtacgcgccg tcaccgatga tgatatgatc cagcactttc
     5221 acgcccatca gctttcccgc ggcacacagc tctcccgtaa attttttgtc ctccgggctg
     5281 ggcgacaggt tcgcgctggg atgattgtgg atacagatga tggcggccgc gaagttctgc
     5341 agggcgtcgt ggatgatttc ccgcacgatg ggcatggcct gattgaccgt tccgactgcg
     5401 gcattgcgga tgacgatcag gcgatgattg ctgttgaggt gggccacttt gaatacctcc
     5461 gtcttgagat cgcgcatttg cggcatgacg atttccacca catccctggc ggagacaatc
     5521 ttgcgctgct cgggtcggac ttcaacttca cgaaaacggc ggccgatctc gagggcggca
     5581 tgaatctggg cgattttggc cggccccagc cctttgaaac cttgccagtc gcgggtgtcc
     5641 gtctggctca tgttccggaa ggtgccgaat ttgtccagaa tctttctcgc caaatcaatc
     5701 gcgctttggc cctgcgcgcc tgttctgagc aaaatggcca gaagctctga attgctcagc
     5761 tcgcgggcgc ctttgccaag cagcttttcc ctgggccggt cgtcttccgg ccagtctttg
     5821 atccccgagg attgaagttt ttcgctcatc ttccgatttt ttcctccttt gggtgaatag
     5881 agaagtccga tttcctccct gtaacggcta ataatctccc ggcgccggat cttcaggccg
     5941 gacagcccgc cagatgccct cagggtcggc ctcactccat tcccttctcg tccggctggc
     6001 gattccaacg ggatcccctt ttttcaaccc cagggaaacg agtcccatga cggccgacgg
     6061 cagagtctcc agcgcctgcc gccacgtctt ggattgccag acgccccctt ttttggccga
     6121 cgagagtgtt ttgtcgccga agcggcgggc ctgccgcatg aagatccccc acaacggtcg
     6181 gggggatcat aggacggcat aatttcgttc aaaaacagaa cctttgtctt tttgacattt
     6241 tttgttgttg gaatataccc ttatgccctg ccgatcgtca atcaacatct ttgtcccctc
     6301 cgggcacatt ctcttgacaa aatcattctt tgcctcgagg atagaatcca aattcaggga
     6361 gcatcatgcg catgtcaaac aacgacgatg ggatctaccg gcagctgcaa attcaactgg
     6421 accggtatcc gatcggctat cccgcgacga aaagcggcgt cgaaatcgac cttctgaaat
     6481 atttctttac cccgcttgag gcaaagatcg ccctgtgttt gacgcttcgc tccatttcgc
     6541 tgacacccat ccgcaagaga ctgaaaggca aatttttcat cgacctttcc cccaaagagc
     6601 tgtctgccct gctggatgaa atgtttcaaa aaggcgtcat ccggcgttcg gaaaacggcg
     6661 gctcctttcg ttacggtatt gccatgctgg ccatcggcat gtttgaatac cacgtcgatg
     6721 atctcacccc tgaactcgta aaaatgatgc atcaatattt tggcgaagcc ttcggcgcgg
     6781 aatttttccg ttcttccctg ccccaactgc gcacaagccc gcatctgaaa acgctgattc
     6841 cggaatacct tgtcgaaccc tacgacaata tgcgccgctt catccaacat acccgcgaaa
     6901 cgatttctgt ggccaactgc gtctgcaaac agggagaggc gctgctggga aagccctgca
     6961 cgcagaccga cacctatgag gtttgtctgc tgatcgggtc aaccggttat gcggcgcggg
     7021 gccgggccag agaaatctcc aaagacgaat gcctgaagat cctggagatg gcggaagaaa
     7081 agggaatggt gctgcaaccg ggcaactccc gaaagcccgc ctgcatctgc atttgctgcg
     7141 gctgctgctg cggcgtgctg accaccgcga agaagcagcc ccggcccgcc cggtttttcg
     7201 caacgaacta ttacgcgcgc atcgacgccg aatcctgcac gggatgcggc gtctgcgtga
     7261 agcgctgtca aatggaggcg attgtagcag acggtcaaac cttccgcgtg gatcgggatc
     7321 gttgcatcgg ctgcggactg tgcgcaaccc ggtgcaaacc ccgagccgcc cggttgatca
     7381 ggaaagacaa gatcaccgtg ccgccgatga acacggaaat gctgtacctg agcatcctca
     7441 tggaacgggc gggacgaaaa aagatgatcg tcaacatgct gaagcttctt ttcggcaagc
     7501 ccttgtgatc gtcagaatgt tccaaaccat ttcagaatca tatcgtttgc ttcacgggga
     7561 taggcatgcg agagatcggg aatcaccttg agcttgatat ccacgcccga ttgctgcaaa
     7621 taccggcagg cctgaaccgt tcgccccacc ggaaaaatcc agtcctgcgc gccgtgaacc
     7681 cacagcacat gtttttcccc ggcccggctg atcttcaccg gtggcagagc gcaggaaacg
     7741 gctgcaatct tagagaatat attgtcccgg gtcagtccca gcgcgagagc aaaggtcccg
     7801 ccgtcggaca ttcctgtcag caaaatgcgg tttcggtcaa cgggaacgcg ggtgcaaacc
     7861 tcctccagat ggcgcatcag catctgcgca tccgtctcaa cgtggccgat ggaccaggtc
     7921 atggcctctg aagtgggagc caaaataaca aacccacgac ttcgcgcctc gcgaatccag
     7981 gtccagataa aatcccgacc atggccatag ccgccgtgca gcgcgacaac cagcgggcgg
     8041 gcggccgttg aggaaggggt tgaaggaata tagatggaat aaccgccccg ggcgcgcagg
     8101 tccctgtttg cgccgacatg aaacaggccg atgccggaat ccccaatggt tgcctcaggc
     8161 aaaacgtctg cgccggtttc cagaaagtag cggtgaacag cggggaaaag gcggcaaagt
     8221 ggaaacagcg cttcctcggc ccggcagatc ttgcgagacg cgcgaagagc cgatataaag
     8281 gcgtcctgaa ggtcatctgc cctgccgaaa tttctgacgg cccggagcag aaaatcgcag
     8341 gcgcgtaaaa gctcccgacg gccatcttcc gatccggcag cgccgtcttc cgtccccaat
     8401 gacgcccgga cagccgccag catgggttca tgaacggcca gttcgccccg aatggaggga
     8461 atcatcagcg gctgcagttt gcgcattccc gcttcgaatg ccgacagaac ggaaaccagc
     8521 gttaccgata tgctttccag attcgctgtc atggcttccg atcgctcagg attttgtcgg
     8581 cggctttcat cgcggatgaa aaactgatgt cgcccgaaca gccgtcccct ctcaccgtat
     8641 cgccgataaa atagagtcct tcgatccccg gacattcgac atccggccgg ttcggcttgg
     8701 actgcgcggg tgtcaggaca ttcccgttca tgaccgttgc gaccattttt ctctcatcga
     8761 cgaccgcagg cagaaaatcc ggaaaaacgg cggaaatgac gcttttcatt ttttgctccg
     8821 ccccggcaac gacgctcgcg tcgccgccga agtgccacgg gagcatgatt ccccaggtgg
     8881 aaagatactt tccggcaggg gtaaatgaag gatcggcgct ggattggaag cgcgcccaga
     8941 tcggcacgtc cacgccgaga atcccggaac gaatgccggt aatgggtctg ttcgtgatga
     9001 aatctataac caggccggaa ctgtgctcga tattcttcgc atatttctcg acagccgcgg
     9061 gcagcagtgt ctggtcgagc agatcggcaa tcaggggaag tccggcggca aaaaggacaa
     9121 ccggcgccgg atagatcgcc cttccggttt tcacgcccgt gacccggcgg ttttcaatga
     9181 taattttttc cgccttttca cgcagatgga tttctccgcc cctgtcgatg tgccggctca
     9241 gcgtttgaaa gattccggcg gagccgccgc gcggctctcc aacgccaacg cccgccgcca
     9301 ggacccgctt taaaaagtcg atcacttcgc ccgcgctgac cttattcgga tcgggacaca
     9361 tgatcgtcat ccccagaaac ggcaggaatg cttcaacttc cggattccgg aaagaggccc
     9421 gatagaaatc aagaagggtt cgatgatacc agtcggcagg atcggttttt ttgattttag
     9481 caaacaatgc cagcagcacc agcctggcgc gtatcgacaa cagaggcgtt ttcaaaaagc
     9541 caatcggccc ttccggtctc tcccaaagcc tgccccggaa aatcagtttg ccgtctttgg
     9601 attcgggcag aaaatcgatc tcctccccga gccttttgaa cacttcattg gcgatgccct
     9661 tgtgcgcgag cctgtgcgaa tgcgccccgt attcccggag atacccttct tcatcccgaa
     9721 acgaccgtgc gcggccgccg accctttcgg attcttccag aaccaccacc ttccggccgg
     9781 catgagacag aagcgccgcg gcactcattc ccgcataccc tgctccgata atgaccgcat
     9841 caggcataac cccctccatc caccgagatg atggatgatt ttcttttagc acttttccgg
     9901 atcggagaaa agtgctgttg cagcggttat gaaaatcccg caggcataag ataaggcctt
     9961 cacaaaggcg ccatattggc gtaaggtttc aactcaatat tgcttcggca gtcaacggga
    10021 cgaaagggag gttgagatgt ccgagcgaaa gcggtttttg ggttgggaaa atccgggcaa
    10081 agcgctgatc accggcgcat cggcaggaat cgggcttagc tttgcgcaac aactcgcaca
    10141 aaggggattc gacctgatcc tgctggccag gcgcagggag cgtctggaaa cagcggcaag
    10201 aaagctggaa tccgactgcg ccatccactg tgagatcgtc accgcggacc tctccgacat
    10261 ggatcaaatc aagcaaaccg ccgacctcct tcaaaccgtc gacggcctgg atgttctgat
    10321 caacaacgcc ggctttgcca ccatcggccc cttcgccggg gttcccctgg agaaatcaat
    10381 gcggatgctt cacctgcaca cgacggcctg tgtgatgctg aagattttca ttcccggctg
    10441 gaaaaaccgt ttttacgtat ggattcttca gcacagcgcc gcggcgcggc ggatcgtcca
    10501 aaacagcgta aaaaaacgaa gccaaaagaa acgttaaaaa gcggggatcg cgttcagatt
    10561 ccggcgaatc ctctttccag atcggcgata atatccttca cgtcttcgat gccgaccgcc
    10621 agccggaaaa gattgtccac aatgccgagg tcgtagcgct gctcccgcgt atagcggtaa
    10681 taggtcactg tggcgggatg cgtaatcagc gtctccacgc cgcccagact gggcgcgatg
    10741 taaatcatcc caagggaatc catgaatctt ttcgccgcat ccagatcgcc cttgatcgtg
    10801 aaagtgacca cgccgcctcc gcctttcatc tgctctctgg caatggcata atgggcatgg
    10861 ctttccagaa aaggataata cgccttttcg attttcggat ggcgctccag atattcggcg
    10921 atcgcgagcg cattgtcatt ttgcgttttc acccgcaggg gaaaggtttt caggcctcga
    10981 agcagcagat aacagcagtg cggatcaatc acgcccccca tggatttgtg cagctcccgg
    11041 actttgccga ccagctctct tttccccagg agcacacccg ccagaatgtc gttatggccg
    11101 gcaaggtatt ttgtcgcgct gtggatcacc agatcgatgc cgtaatcgag cggccgctgg
    11161 ttgaacggcg tggcaaaagt ggaatcgatg atggtgatga ccttgtgctt atccccgatt
    11221 tgcttgaatt tgaccatgtc aaacacattg aggtacggat tggtcggaga ttccgaaaaa
    11281 ataaaacgcg tattcttctt gacggcggca tccagaacat catagtcgcc gaacggcacg
    11341 atcgtgcatt cgatgccgaa ccgtttgagg taggatcggc aaaattccag cgtttttcca
    11401 tagcaatcgt cggtgatgac aatatggtcc ccggtctgca ccagagcaag aatcgtggtg
    11461 gtaatggcca tcatgccgga agaaaacatc aacccttcct gggcgccgtc cagctccgcc
    11521 aggcgccgct ccgccacctt ttccgtcgga ttgccgtatc ttccgtattc ataacgcttt
    11581 ttatcgttct tggtgtaggc ttcaatttcc ctgctgtcct taaaggaata cgtggatgac
    11641 tggacaatgg gggtggtaat cgagtcggca tagcgcgccc ggtcctcccc tgcatggatg
    11701 gactttgtaa aaatggattc tgattcatct tttttcttca tggcttcctt tccatcaagc
    11761 aaagaagagg aacatggctt gctttcatac gaaggcagta taaaaagaag gtccggggat
    11821 gtcaatggac aatttcgcgg atggtcgggt ggaatagacg gccctcatgg gtattgactt
    11881 ttttctcccg aatcactaga ttgaccgaga aaggaactga aacctatgcc gatctatgaa
    11941 tttgaatgcg acaaatgcaa gaagcaattt gaagtggtca cgttgagcct cagcgaaaag
    12001 ccgaaagcag tttgtccgaa gtgcaaatcc aaaaaagccc acaagctggt ttccaaggtc
    12061 ggaaagggaa agtacgggtc attgcgctcc ggcggctcgc tcgacaccgg cgctccgtcc
    12121 tcttcaggat gttcgtcctg ttcctccaca aactgctcgt cctgcggtca ctaaagcccc
    12181 gtttttctgt aattgacgaa ttcctttaat aaggtataat acggtcaact ccggttgtat
    12241 tgatttgcct ctcttcttct ttcctgacgg aactgtgctg gaattttctg attttcatgc
    12301 ctcttttttt gaaacacctc tttttgttcg gcgtttgctc atcgtgcagc atctgaatgt
    12361 ccgggaaatt ataagctaac gttttctttt tggatttgag gatctctgcg tttttcggca
    12421 atagtgcttg ccgtcgggta agtgggaaaa tcacagccga tacggcgggt ctgaggcgtt
    12481 gacatcttta taaccccttg agcagaaagg agattgaatc atgagcagca tttacaaggt
    12541 tatcgagatt atcgggggaa gtgaaaagtc atgggaagac gcggccaaaa aagccgtgga
    12601 aatcgcagcc aagagtctca aggaaatccg agtggcggaa gtcaaagaac tggatatgag
    12661 aatcgaggaa ggtaaggtgg ttgaataccg cgtaaaattg agggtttcct tcaagtacac
    12721 gccgtaaaag cattttgctt ttgcgcgccg atgtcgcggc ggacaacgtc aacattccat
    12781 ctggcaatta agaagagcgg cggaaatagt tccgccgcgt cgggggggtg agactgtgcc
    12841 gcaaaaaagt catcaattgc agtggttgca atgctccatc tcacgggccg aagacggcaa
    12901 gagctggatc gtggtgtgat caatgccgtg atcttcatga aggatcttct gcacggctcg
    12961 gataatttgt tcatgatcgg gcgcttcgtc atcggacacc agatgggcgg aaagcagttt
    13021 ctggccagtg ccggtctgcc agatatgcag atcattgact tccgccacat ccgaaacgga
    13081 aagcaaatct ctttttacag cgttgaaatc gacatccgcc ggaacggaat ccagaagcgc
    13141 gttgacggtg tgccggatga ttttgtagat ttcgcgggca atgaaaatgc agatgaccat
    13201 cgagatgagc ggatcgataa aatataagtc aaagtgcata atcagcaggg cgccgacaat
    13261 cacccccaga gaaaacagcg catcctgaag cgaatggagc caggcgcttt tgatgttgaa
    13321 gttccgagcc gatattttct gtagaagcag cgtggccgcg ctgtttccca caaaagccac
    13381 cacggcaacc caaagcatca tcgtgccgga tacctgcgca ggtgaaaaga gtctttgaac
    13441 agactcccag aaaacaaaag caatcaccac cgacagaaca atgctgttga cgaacgcggc
    13501 gataaattcc atctttcgcc agccgtaggt tttgtgttcc gtcgccggac gccgggacat
    13561 cttttccgcc caacaggtaa agctcatggc agcaatgtcg ctcagattgt gaacggcatc
    13621 cgagatcagc gccatgctcg aaatcagcaa gccgaaaatc atttcaaaaa cgacgatgcc
    13681 gccgttgatg agaatggcca gaatgagatt ggcaagcgac ggcggttgat cattgtgatg
    13741 atggttgtgg ctcatttcat taatctttca tgtggttttt tatcttgtat caaaccatca
    13801 accagccgta agccatgccg gcaaccgttg aaagcagcac aacgatggaa cagtacacaa
    13861 ccgttttcct gattcccatg atgctggaaa tagcgatcat gctgggaaga gaaagcgccg
    13921 gtcccgccag caagagcgcc agcgcaggac cctgccccat gccggagccg atcagaccct
    13981 gaagaattgg cacttcggtc agtgttgcaa aatacatgaa agcgccgacg accgcggcga
    14041 aaaaatttgc ccacagggaa ttgccgccca ccagcgcctg gatgtactgc tcgggaatga
    14101 gcgccggata accgggacgg cccagcaaaa atcccgcaac caaaacgccg ccgaacaaaa
    14161 gcggaagaat ttgcttggca aaatcccagg tagcctgtac ccaaccttgc agttcttgtt
    14221 tgacaaacca ctttttgagc atgatcccta aaacgaccag caaccccatc accaaaaacc
    14281 atttgacgct gaagatcatc cgccagaggc cgccgtcatt ctctgtcggt ccggcaaacg
    14341 aggcaaaaac cagaatcagc accatgacca gcatataaac ggcatcctgc caaagcgccc
    14401 gtcccttgcc ttcctcctcc ggcagaaaca tttcccccgt ttcccggttg gcgtcgtctc
    14461 tgcggaaaat aaaggccatc aacaaacccg taatgatgga aaatgcaaca gcaccgacgg
    14521 cgcgagccat gccgagctgc caacccagca cctttgccgt gagaataatc gccagcacat
    14581 tgatcgcggg cccggaatac aaaaacgccg tcgccgggcc gatccccgcg ccgcgcgtat
    14641 aaatgccggc aaaaaggggc aggaccgtgc aggagcaaac cgccagcacg gaaccggaaa
    14701 cggaggccac cgaataggaa agaattttat tggcggacgc accgaaatat ttgagcactg
    14761 ccccttggga gacaaacacg ctgatggcac cggcgatgaa aaatgcgggg atgaggcagg
    14821 tcaatacatg ctgtctcgcg tagtcctgaa gcatcatgaa tgcctccagg acggcctggc
    14881 ggacgaccag ggattcccac ggaataaaat aggcgcccag aaagatcacc gcaatccagg
    14941 ccagtttttg ccactctttc atattgacca acctttacgg aactctaaaa tatttttgtt
    15001 gaaaatgcag agcgaccgtg accaaaccga tcatcaccgg cacttcgacc agcgggccga
    15061 tgacggcggc aaacgccgcg ccggaattga taccgaaaac ggcaatcgct acggcaatgg
    15121 ccagctcaaa attgttgctc gcggccgtaa aagccagcgt ggttgttttt gaataatccg
    15181 cgcccatctt tctgcccaga taaaacgaca cgagaaacat gactacaaaa tagatcaaaa
    15241 gcggaatggc aatccgcacg acatcaaaag gcaggctgat aatcaaattg cctttgagac
    15301 tgaacatcac cacgatcgtg aataaaagag cgatcagggt taacggactg atgaccggca
    15361 caaatcgttc gtgataccgc ttttttccca caagctttac gccaatgagg cgcgtcaggg
    15421 cgcccaaaat gcaggggata cccaggtaga tgaagacgct ttcggcgatc tgccggatgc
    15481 tgacatcgac gacgctgccg gaaaatccaa aatacggcgg aagaaccgta atgaaaaacc
    15541 aggcaaagac gctgtaaaag agaacctgaa aaatgctgtt aaaagcgaca agccccgccg
    15601 cgtattcggt gctgcctttc gccagctcat tccagacaat caccatcgca atacagcgcg
    15661 ccagaccgat catgatgagc cccaccatat actcgggctt gtccggcaaa aacacaatcg
    15721 ccaggataaa catcaaaacc ggtccgatca gccagttttg cgcaagcgac aatcccagca
    15781 ctttcccatt gcggaatacc tgaggcatct cctcatattt caccttggca aacggcggat
    15841 acatcatcaa gatgagtcct atggcgatag gcacattggt cgtgcccacc tgaaatgaat
    15901 taataaaggc ctcgacgccg ggtataaaat aaccaagtcc cacacccaga atcatcgcgg
    15961 caaagatcca aacggtcaga taacggtcca gaaaagacag cttgtcggca ataggctcag
    16021 acatcccaac cctctcttca taccctattt tatttccaga tcaaaaaaac gcgcttcctc
    16081 attttacgat ccagccccgg atctgctctt cagtcggcat tttgcctgcg atctttacct
    16141 gaccgtccac gaccagaccg ggcgtcgtca ttacgccgta ggtcatgatt ttcttgatgt
    16201 ctttcacctt ggatatttcc gcgttcacag ccgattcttt cacaacttca ttcaccagct
    16261 cttcgagttt caggcacttg gcacagcccg ttcccagaat ttcaatcttc acaacaacct
    16321 cctatttttt gcaggatttc atcatttttt tatggtcttc ggcgttggcg gaaaggacct
    16381 cctccacaca ccccataaaa ttaagaatac agggacagcg cagacggtaa tagatgcagt
    16441 tggcccggcg atcgtccgcc accagccccg cattttttaa tacgcttaag tgtttggata
    16501 ccgtggaggt gtccgtcccg atcatttccg tcagctcgtt gacacagcgc tcctgctttt
    16561 gcagctcctc aatcatcaga agacggctgg gatgcgccat cgccttcagg atcttcgccc
    16621 gggcttcata ccgcggcctg agagattctt tcatattcgg tctctccttt cgtatttggc
    16681 catatagcca aatagccatg ttttgtcaag agggaagcga gaaggaattt aacgcaacgg
    16741 aaaggatcaa aaaaagaggg gggagacgtt tttcgtctcc ccccggatgt atccaagacg
    16801 actctgttgc ctcgggatca gtccagaaca accataacgc ggcacttctg caggaaggcc
    16861 aggttctgtt tcgcggagcg gatctttccc gcgtcttcgt tgctgatgac aaattccgac
    16921 atgccggcgc cctgagcgcg gatcgccttg acggtcagcg gactgttcgt cactctggcg
    16981 ttgctttgcg cggaggtcag atcacgggca taaccgcaga tgccctgctg catggcgtag
    17041 gttttattca cgatcagcgt cccgtacact tcagagccgt tttcgtcaaa aatcttcggc
    17101 gacatggccg ggcgagcctg caaaccgcgg ccgtcgatca ccaagccggt atactcttca
    17161 tccttcggag cgggcttttt ggaaaaaggc gccggtttat gctcccggtc ttcctttttg
    17221 tcatcggcaa tggccttggg aagaatggct ctggcaactc ccgagagcgg catgcgcacg
    17281 gtcacttcca cggtaccatc cgacaggtac tcctttttta caatttgcgc gcccttcacc
    17341 atgccggaaa ccgccgtatt aatcacgtcg ctctctacgg caaaatcctt gacggtcgtc
    17401 tgtgaatcga tctgaacgcc ctgcacggtt tccagcaggt ttctgaaggc atcaacctgc
    17461 gccgcacgca gcgccatggg ccgcgcctga ggttttccgt aatagcgctc aggcggcgcg
    17521 ccggttccca cggattcgat gtacccgtcc tcgtaattga tcagtccctt gtctccgatc
    17581 tgattggtga ccttggattc aatgattttt gtccaatccg acatgccgac cctgccctcc
    17641 tgggcaaaga caacgcaggc gcacagaaga agcgcaccga ccgccagaaa aattcccgct
    17701 ttacgcagcg tattcataac gaacctcctt ttattttcaa caggactgtt tttttgctga
    17761 tgacgacgag tgctttatac cacagttcga gccaaaaaat gatttttttt cggccggcaa
    17821 cgaaaacaga ccatcgtcgg ccgttgatct tcatccattg cagccgttac aaaagaaaaa
    17881 aaaccgtcac ggatcaacac ttcagccatt acaaaatgat tgaaaaatga agcactttcc
    17941 tttacaaatc ctcccatgat cctctttggc ggaaaatcgg gaaaacgtct gcccggaaca
    18001 ggttgtttgc ttgcgcttgc agcccttctt tcgcttgccg cctgtactgc gcgcacaacc
    18061 gtcgcggacc gactggcgcc ttatgcacaa gccaccggaa tggaaaatgc cgggcagctt
    18121 cttctggtaa cggatgaaag ttttcttttt ctaacttccc ataagattta tccactggaa
    18181 aagacgaatg ccttatggca acaggccatg gcgcccatgg acgccgtcat cggcagaaac
    18241 ggcttcgcgc cgccgggcga aaaacgcgaa ggggacgggc gcacgccttc ggggctcttc
    18301 cggctgaaaa ccgcttttgg ttatccgccg tcggcgccca cccaaatgcc ctaccgtcag
    18361 atgctggagg acgacttgtg gatcgacgac ccgaatgacc cggattacaa ccgcctgatc
    18421 aggcaaaacc agaccaaggc cgcctcctac gaaaaaatga agcgcgatga tgatctctat
    18481 aaatacggga tcatcattga atacaatacg gatccggtca tcaaaggact cggcagcgcg
    18541 attttcctgc acgtctgggc cggcgcgaat tccacgacgg ccggatgcgt cgccgcatcg
    18601 gaagacgaca tcctgaaaat tctcggctgg ttgaatccgg cgaaaaatcc cgtcatcctt
    18661 atcaatcctg acgaaccgta ggtgaaggag gttttttcat gaagttaaaa ttatttctga
    18721 tcacccttct tgccgcaatc ctgttttccg cgggagagcc tcccgcgtcc ttttgcgaag
    18781 aggtcatgcc cgacacgttc gtccacattc aggaaatcat ccccgacgcc cttctggata
    18841 tccgctattt taccgaacat aattttctgg gcgtcaaagt ggacggctac cttgcgccca
    18901 cctgcattct gacccgtccg gcggccgaag cgctggcccg ggtccaggaa gacctcgcac
    18961 ctttcggcat gaccatcaaa atctatgact gctaccggcc gcaaaaggcc gttgaccact
    19021 ttgtgcgctg ggcgaaagac attaacgata ccaggaccag gaaagaattc taccccacgg
    19081 tggacaaacg ccatcttttc cgcgacggct acattgccga gcgctccgga cacagccggg
    19141 ggagcacggt tgacctgacc cttgtgccgc tgccggcccc gattcagccc tcttacaagg
    19201 ccggcgaccc cctcaaggaa tgttatctgc cggccggtgt tcgcttcgcg gacaactctc
    19261 tggacatggg cacgggattc gactgcttcg gggagatgtc ccaccccgaa aacaaagaca
    19321 tcggcccgca gcagagagcc aaccgcctcc ttctgaaaac gttgatggcc agacacggct
    19381 ttcggaacct tcccgaagag tggtggcatt tcaccctaaa caatgaaccc tatccggaca
    19441 cgtattttaa ctttccggta aaataaacat gaaaatcaat ctgccgccgt ttctgagatc
    19501 ttacggattt tcgattctgc tcgtcgcgtc ggtttttgtc ggcgccctcc tcggcatcct
    19561 gtatcaaaag gacgccgccc gtctcaagcc gctgggcgat ctgtttttga atctgctgtt
    19621 caccgtcatt gtgccgctgg tgttcttttc catcgcctcc accgtagcct ccatgaccca
    19681 cctcgggcgt ctcgggaaaa ttctcctggt catgattctg gtgtttgtga tcaccggcct
    19741 gatcgcgtcg gcggtcatga tggtcgccgt tactttcttt tcccccgcct ccggcgtcag
    19801 catccccctg ccgacaaacg ccgacgttca acaccttaag gcaggggacc aaatcgtccg
    19861 ggccttctcc gttccggatt ttcgggacct gttatccaaa aacaacatgc tggcgttgat
    19921 cattttctcc atgctggtcg gcgtggcggc gtccgccgca ggcgaaaagg gaaaagattt
    19981 ctcaaggttc ctcacctcgg ccaattcggt catgatgaag ctcatctcct tcatcatgta
    20041 ctacgcgccg atcggcctgt gcgcctattt cgcctgcctg gtcggcgttt tcggcccgga
    20101 gcttctgggc tcctacgcgc ggacagtggt cgtttactat ccgacggccc tgttatactt
    20161 cttcatcgct tttacttttt atgcatacct cgccgggcgc ggtcggggcg tcagagtatt
    20221 ttggagcaac atcattcccc cgtccgttat gtcgctggcg accggcagtt cgatggccac
    20281 cattcccgcc agccttcagg ccgcggaaaa aatcggcgta cccaaggata ttcgtgaggt
    20341 cgtgattccc atcggcgcaa cgatgcatat ggaggggtcg tgcctcgccg ccgtgctgaa
    20401 gatcgctttt ctgttcggca tcttttcaat gcccctgacc ggcgtcgata cgattctgac
    20461 ggcgctgggc atcgcgcttt taaccggcgt ggtggtgagc ggcattcccg gcggcggcac
    20521 catcggcgaa ttgctgattt tatccctgta cggtttcccg ccggaggcgt ttccgctcgt
    20581 caccatgatc ggcacgctgg tggacgcgcc ggccacgatg gtcaacgccg tgggcgacaa
    20641 tgtctccggt atgatggtgg cccgggtgct gggcggaaaa gagtggatgg acgggtaaat
    20701 cttcagggca aacagacgta aatacgtcaa ttaaattgag atcttatcgt ttttgcgaac
    20761 gcagatcgtt ttgaatctcg ctcacttcct tcttgacctt ctgatacgcc aaaaaactgt
    20821 gcaggaacca accgaaaaca aacccgaaag ccaggacaaa gaaaataagg agtgcaagag
    20881 acagggaaat ttcccagaaa aaaatactga ccgtcacggc cgacacattc tgaatgacga
    20941 agacaacggc aagacaggca attacgatga gaaggacaat cttgaaattc atctctcaaa
    21001 ctcccggcaa gcagattatc ggttgatttt ataaaccagg cacaaacgcc cttcggaaaa
    21061 ttacggcata tccgctgcga atgcaaccca aatttattca tcaaatacaa agaagccccg
    21121 cggctttcga ccgcagggct tctttcaact tagcgtttat ctttcctttt tgccatgagc
    21181 catgaacttt gagcttttca ccatcagcca caggggctga atactcgtac tctgttttac
    21241 tcgccgctca ttgctcgtgc ccgttcttcc gcaacctgtt agaacgtcag cgtcagcttg
    21301 ttgatgacca ggaaatcgtc ggtcaattca ttgttgacgg ccacattggc ctgatagtat
    21361 ttgccggtga acagatagcc ggcgcccacc atgtaggaca ggttgttggt gattttgtag
    21421 gttgcggtca aatccacttc ccatccgtaa tcatcgttga ggtactcggt ggccggattg
    21481 ttggctgtgt tccagggctt tttatcggca aatgcataac tgacggaagc catgacatcc
    21541 agcttttcga ccggcttcac gccgccgcgg agctggaaga accatgcgtt tgccatctga
    21601 ccattgtcga ctgaggtacc gtagccggtg agattgccgg cccacttggc ccgatcgtaa
    21661 tagctgaaca tgatcaggca gggattccag tcgcgcccgc cgttgatgag tccgccctcc
    21721 gttttgtcct tcgaatccgg atcatcaccg gatacgtacg cgatgctgcc gccggcgtaa
    21781 aacatgccga aattggccgt tgcgtcaaga taggcggaaa tgttttccaa ggtcactttg
    21841 tccacaccgg ccactttgac tacgccgtct tcatacttgt ccgtaccgtt gagatagttg
    21901 aattcggcct gaacggaaac gggaccgaat ttagccatca gatacggcgt gaacaggaaa
    21961 taagtccttc tgtagcttcc gttcgcggcg ggaaccgccg gcgggcgtgt gttggcataa
    22021 ttgtaataat tgacgttcaa accggcgcgg ccgcttttcc agctgtaaat gccttcgagg
    22081 tgataaacat cattgtccga atcggcaaaa gcggccgtag caggaggaac aggattgata
    22141 gctgtcttgc tgttttcttt tactttggaa atagaagccc acacattaat cgggccgaac
    22201 attctgttga accggattct tgcctgcggg gcgctgctgt tgccgaaaat cgtgcccgtg
    22261 cttccatagt tcatgtaacc gaccagaaac gtgccgatgg ctgacttgta atcgatgtaa
    22321 acccaatcga tagcgatgtt ttcattttcc gccactgttc ccgcggaatc gactgccagc
    22381 gccgttccgg tggcgcttct ggtcgcgccc caggctcgct ccatcgcgtc cagacgcgtg
    22441 atcagggtca atcccggaga aatggtgaaa tccgttctgg ctctcaatct ctgaaagtaa
    22501 aaagcggtgc tgggatcgcc gcccgttgtg ctcttggtta aatttgtttt atccagatac
    22561 attccacccg cgtaaaactc tccgctgaac ttgacgtcaa cggccatcgc cgtcgaactg
    22621 aaggcgatga tcagccccag tgacagcata accaaccaaa atctcttcat gttctcctcc
    22681 ttacatatga tttatcctga agctcaggtt ttcattggca attacgcctg caatccccgg
    22741 ttcaccttgt tcattgcaag gaagccgcca gaccaaaaac gagcccggcc ttcggg
//
LOCUS       DAOSRM010000025        22736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2449_length_22736_cov_116.296945, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000025 DAOSRM010000000
VERSION     DAOSRM010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 22736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22736
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2449_length_22736_cov_116.296945"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..978)
                     /gene="mutM"
                     /locus_tag="PLA58_04030"
     CDS             complement(202..978)
                     /gene="mutM"
                     /locus_tag="PLA58_04030"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOR61996.1"
                     /translation="MPELPEVETLCCQLQGKIAGKKILAAKVFDHKLDGIANVRGMRI
                     CGVCRRGKTVEMRLHDGRSIVVHLRMSGRLFWRADRKKEPHTRWRMTLVDGNLDLVDP
                     RRFATVRVVHAGRESLSNDLMGRFDFRNFMQSQAGRRIHVKTLLMDPKAIAGIGNIYA
                     CEILHAAGISPLRPAASLGESEWKKIFRQARRILRKAIAGRGTSISEWRDLYGCPGEN
                     QKELKVYGREGEDCVRCGGIIVRIRQGGRSTFYCPRCQEE"
     gene            1163..2473
                     /locus_tag="PLA58_04035"
     CDS             1163..2473
                     /locus_tag="PLA58_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOR61997.1"
                     /translation="MENETLFNEDAEPPGPSAGLVVVPNKTTNFFSLTTNNHLPSLAK
                     SKPERLYFPFKDSPRSRAFRKKHYPDISRIEWFDWRWQFQNAIRHVQTLDAMVHLSDN
                     ERQAMMHPSGDLPVAITPYYASLISPEDPSSPLRRCVIPVVDECLHTKGEEEDPLGED
                     ADSPVPGIVHRYPDRVLFLVTDICSTYCRYCTRSRIFGRQHQCFIDTAKWEKALDYIA
                     SNPNVRDVLLSGGDPLTLSDDRLEWLLSRLKEIPHVELVRIGTKVPVVMPQRITPALV
                     SMLKKCHPLWMSIHVTHPDELTPEMSAACIRLADAGIPLGSQTVLLAGINDSVSTMTR
                     LVHGLLQIRVRPYYLYQCDPIPGSSHFRTPISKGLEIIQGLRGHTTGYAVPTFVVDAP
                     GGGGKIPLLPEYAIGWDKGDLMMRNYEGNIFRYPDNHAESKDIH"
     gene            2490..3563
                     /locus_tag="PLA58_04040"
     CDS             2490..3563
                     /locus_tag="PLA58_04040"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR61998.1"
                     /translation="MSGNPVCPSTPPCRTVTIGLTYDLRDDYLREGYSLEETAEFDKP
                     DTIEAIENVLKKNGHATDRIGHVKALIRRLAAGDRWDLVFNIAEGMHGFGREAQVPAL
                     LDAYRIPYTFSDALGQALTLHKGMTKHIVRDMGIPTPDFAVVSSPEDIGRVLLPYPLF
                     AKPVAEGTGKGITARSRIDNAESLRSVCGTLLQTFCQPVLVETYLPGREFTVGIIGTG
                     KNARVIGAMEVLLNPDAEPHAYSYDNKEHYENLVRYTAVSDPEALRAMEVSLAAWRGL
                     DLKDGGRVDLRSDAFGSPHFIEVNSLAGLNPVRSDLPILCGLAGISYDQLITDILNSA
                     LERAGLSRGQSLQPDGAGTEKSP"
     gene            3560..4546
                     /locus_tag="PLA58_04045"
     CDS             3560..4546
                     /locus_tag="PLA58_04045"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOR61999.1"
                     /translation="MKKILILHSDVPADAGEDELDCLTQARTIGEAIISLGHRPEKLS
                     FGMDLQANISRIREHQPDIVFNLVETLAGKGSLNHFATALLDFLRLPYTGCRTDAMFV
                     TSNKPLAKKMLRQAGIATPDWITLEDETESPSPGEVYFLKPCREDASVGLDDRAVVQM
                     QNPQTIRDTLRERQDALGLDCFAEAYIDGREFNIALLASDQGVRVLPPAEILFLDYPP
                     DKRKVLDYRAKWVEDSFEYSHTVRSLSIAPQDERLADRLRDIALRCWHLFGLRGYARV
                     DFRVDGNGIPWVLEVNANPCLSPDAGFAAALDFAGIEYTGAIDAILRDALKP"
     gene            4565..5050
                     /locus_tag="PLA58_04050"
     CDS             4565..5050
                     /locus_tag="PLA58_04050"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR62000.1"
                     /translation="MKTITYRQEIRPTDPDAIAEIVRGSGFFSAAEEALARELAEERL
                     EQGEQSSYRFLFAEEDGRVVGYTCYGLIPATAGSYDLYWIAVTQSLRGQETGKRLLQK
                     TEEMIRDLGGRNIYAETSSRPQYAPTRHFYEKCGYLPEAFLKDFYSPGDSKIIYSKPL
                     I"
     gene            5182..5802
                     /locus_tag="PLA58_04055"
     CDS             5182..5802
                     /locus_tag="PLA58_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOR62001.1"
                     /translation="MRLFPKEENFFEHFEELANKIEEGGQFFLEMTRNRDYSEAKVAR
                     LKELEHEADGITHKTYERMHKTFLTPIDREDIYALVNKMDSIMDVIEATAVRIYMYKV
                     KKPDDEIIKQAEILFQAIKKIKGVVHGLRDMKNSQMILDGCVEINTLENAGDVVLRTI
                     ITNLFIKEQDAIELIKWKEIFERIEEAIDVCEDVSNIVEGIVLKHA"
     gene            5795..6793
                     /locus_tag="PLA58_04060"
     CDS             5795..6793
                     /locus_tag="PLA58_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOR62002.1"
                     /translation="MLDSMAFVFILVAIALIFDFLNGFHDSANSISTVVSTRVLSPKY
                     AVLWAAFFNFAAVFFVGTQVAHTVGKGIIHIDIVDNLVILSALGGAILWNITTWYYGL
                     PSSSSHALIGGLIGAGVTKAGTGTLVWSGIIKTSLFIIISPAVGMALGFFFMALAMNL
                     SRNSNIAKSDKVFRKLQLFSAAVYSMGHGMNDAQKTMGIIAMALFSKGLLGDTFHIPI
                     WVVIICYTMISLGTMFGGWRIVKTMGTKITKLQPIGGFSAETAAAFSIIGATMGGIPV
                     STTHTITGAIVGVGSTKRLSAVRWGVAGNIIWAWILTIPISAVIASVIYLAVNSFV"
     gene            6889..8154
                     /locus_tag="PLA58_04065"
     CDS             6889..8154
                     /locus_tag="PLA58_04065"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOR62003.1"
                     /translation="MPEKRLPFTRKQLSAILEKYPTPFYIYDEKALRKSARAFAKAFS
                     WNRGFKEFFAVKACPNPYIMKILHTEGFGADCSSMAELLLAQKVGITGEEIMFSSNDT
                     PAAEFVQARKLKATINLDDLSHIDFLEKHAGLPDLICCRYNPGPLKKGNIIIGHPEEA
                     KYGFTREQLFEGYRICRDKGVRRFGIHTMVASNELDPDYFVETAEILFHLIVELSHAL
                     NIRFEFANLSGGFGIPYRPEQKAIDLEVMTRGIREKYEKIITAGGMAPLKIFTESGRF
                     ITGPHGYLVAKVLHIKDTYKKFAGLDACMANLMRPALYGAYHHITVVGKERRPHDILY
                     DVTGSLCENNDKFAIDRLLPRLAPGDTVVIHDTGAHGHSMGFNYNGKLRSAELLLRED
                     GSVIQIRRAETVKDYFATLDFKRLSRFKG"
     gene            8222..8815
                     /locus_tag="PLA58_04070"
     CDS             8222..8815
                     /locus_tag="PLA58_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HOR62004.1"
                     /translation="MTAHLAPFRSTFNLQSYAFSLYPFILAFSHPRHYIAGMAKEKIP
                     STPAVLVLKKSAATFTLQTYAYEEHGGTKVSSRKLGVDEHCVIKTLVMEDENANPFMI
                     LMHGDREVSTKALARALGVKTVRPCTPETAHRHTGYFVGGTSPFGAKKDLKLYMEKTI
                     SGLPEIFINAGSRGLLARMPPSELVRILNPFEVSVAI"
     gene            8871..9428
                     /gene="trxC"
                     /locus_tag="PLA58_04075"
     CDS             8871..9428
                     /gene="trxC"
                     /locus_tag="PLA58_04075"
                     /inference="COORDINATES: protein motif:HMM:NF008229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HOR62005.1"
                     /translation="MPENVIIRCLNCGTKNRIPQQKFQRRPTCGHCHAPLDQLIIRCL
                     KCGTKNRLPEERLSSKPFCGKCGEALVVATQNIQPVEVTDDSFAREVLSAEISVLVDC
                     WAPWCGPCKSLAPTIDELASDYANGVKVAKLNVDENPATATQYGIRSIPTLLFFRDGR
                     LVERLVGALPKQEIERHLLSIIKTN"
     gene            9449..9973
                     /locus_tag="PLA58_04080"
     CDS             9449..9973
                     /locus_tag="PLA58_04080"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOR62006.1"
                     /translation="MDKNRTLAYACKIFSVWEEDVLLQNGKKTRQSWIEHKPTVAVVP
                     VNDQKELLLIRQFRIPTGKNLLEIPAGSMDHGEESPASCAGRELAEETGWRAKKLTPL
                     FAGYLLPGYCNEFMYFFLAQDLVYEPLQPDEDECIEVIPVSFDRAAALLKSGELNDAK
                     TALGIMLAERYLKL"
     gene            complement(9976..10509)
                     /locus_tag="PLA58_04085"
     CDS             complement(9976..10509)
                     /locus_tag="PLA58_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62007.1"
                     /translation="MRHSKKLVFLILLLLMLCGCSAKAVRDGGQESPAPVKKAGSLFS
                     SGPSDRDLFDEALALLSGSGREPDYHEARIRLGTLVEQFPESKWAEGAKALLSMLDRV
                     SALQVQLKEQKAEAQQEQAKLKKEIEGLKSSVRQAEEKNSAELIRLQQENEQLKNDIR
                     QLKNLEIRLEKREKLLR"
     gene            complement(10506..11969)
                     /locus_tag="PLA58_04090"
     CDS             complement(10506..11969)
                     /locus_tag="PLA58_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOR62008.1"
                     /translation="MLRAKGSDGEWHFIPQDHPSARVLEKMSLTISRKLFLGYLFMAL
                     LTVLASAFALFNLQKLSAGAYNITNHHFVLMENSKQLMDILLAQESAEKKYLILRDPE
                     LEQIFWKRSVEFTEKLDETSRLKLDRRTKSAVAEVDVLHRRLKELFSEETLLAQNGHL
                     QEAQAISDTTGRAVIDAMAMQLRDIQKNMDQAINNEMNRIASQGRAAVQMTLGLSLLS
                     LVLGITLALIITRNISKPLKQLQKATRLIAEGNLDHKIEARRNDEIGALADSFAHMTQ
                     RLKVLEALNLDASPLTGLPGNLAIENRIEQSLAKGKLFSLCQVDLDNFKPFADQYGYA
                     WGSEVIKEVAGLLQGYIDEMKLTDMFLGHIGGDDFVLIGDPGQVEGICKKLVVDFAGR
                     ILRYYRPEDAQKGYFIGRNRQGTVQKFPLITITAAIVTDDGSRFKNPLEMARRVAELK
                     EYAKRMPGSNYVTEEAVERQKAAQPQNLPSSLEGSNC"
     gene            12184..13482
                     /locus_tag="PLA58_04095"
     CDS             12184..13482
                     /locus_tag="PLA58_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOR62009.1"
                     /translation="MMYNDEFETLPREALKALQIKRLQQVLQRVCATVCFYKKSFAAA
                     KISPDAIRSLEDLKKLPFTTREDLRNNYPFGLFAVPMSNIVRLHASSGTSGRSSVFSY
                     TRRDLDTWTDLTARSLAAAGLTKNDMVHNAFGYGLLPGGLGLHYGIEKIGASVIPISD
                     GNTKRQITILQDFGPTVLCATPSFALHLAEEGRAMGVDMQSLQLRVGIFGAELWSNAT
                     RDAIEDAFNIKALNIFGLSEMMGPGIAMECLEGRRGMHIYEDHFIVETIHPETGDVLP
                     EGEEGELVFTSLTREAFPLVRYRSGEISRLIPEPCRCGRTHIRMERVLKRSDDMLTIR
                     GINIFPAQVEAILKAIDGVEPDYQLIIDKVGALDAVELHVEVGEKFFGEAGGVKELQT
                     IEKRIVKDMKDYLSISPRVKLVAPQTLKESGKKVIDRRTI"
     gene            13567..13998
                     /locus_tag="PLA58_04100"
     CDS             13567..13998
                     /locus_tag="PLA58_04100"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOR62010.1"
                     /translation="MKVEQISVFLENKPGTLEHATRILKENNINIRTLSLAETADFGI
                     LRLIVNDVEKTNKVLREAGFRVSKTVVVAVEVPDQPGGLHSIMEVLNRETINVEYLYA
                     FVEKSGLNAVIIFRFDAPEKAIEVLLKHKFTVVPGSKLYEF"
     gene            complement(14321..14638)
                     /locus_tag="PLA58_04105"
     CDS             complement(14321..14638)
                     /locus_tag="PLA58_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOR62011.1"
                     /translation="MKKKYKVDITATAEADIAELWDYIAQDNPDAATSFILQLEEQIA
                     SLEQYPERCPLIPENEFLDRSYRHLLFGNYRTIFKMIDTQVIILRVIHGARLLALQTA
                     TAP"
     gene            complement(14622..14939)
                     /locus_tag="PLA58_04110"
     CDS             complement(14622..14939)
                     /locus_tag="PLA58_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOR62012.1"
                     /translation="MAHNRALKRRLKMTQISPSEDIRSVTELKRNTREILNHLHATGR
                     PVILTVNGRADSVLLDVRQYEKNLRAVNLAKLIAPAEQDVEKGKIRSAQEFLKEFKRE
                     KEI"
     gene            complement(15026..15640)
                     /locus_tag="PLA58_04115"
     CDS             complement(15026..15640)
                     /locus_tag="PLA58_04115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOR62013.1"
                     /translation="MKLLLFDFDGVLVDSLDVYEKTVTDCLSAIGRPLARGREEFLEL
                     FEGNFYQSLEDKGVDMKKFSDASVDILAKVRYADMKPFHAIRPVLQELKKNHPLIIIS
                     SNDAPTIKEALRLYDFNGIFDDILGCDFMLSKKDKILHVIQKYNVTLQDIYYIGDTTG
                     DMKEGKQAGVKTVAVTWGWHSREKLAATGPDYLLDRPEDLLQLN"
     gene            complement(15767..16816)
                     /gene="mtnA"
                     /locus_tag="PLA58_04120"
     CDS             complement(15767..16816)
                     /gene="mtnA"
                     /locus_tag="PLA58_04120"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOR62014.1"
                     /translation="MTLIKTVFWKKNAVVLIDQNALPLVERQVVCSDYRQVIAAIRDL
                     TVRGAPAIGVAAAMGAALGALAFASEPIEKFKKKFNAICDEIAAARPTARNLFWAMER
                     MKKHLDEVVRSGKRNPARELVREAKRICAEDIAVNREMGRRGAALFSDGDHVLTHCNA
                     GALATAGYGTALGVLRAAAAEGKKLHVYVDETRPVLQGARLTAWEMKKEKIPATLICD
                     NMAGSLMRQGKINKVIVGADRIAANGDAANKIGTYALAVLARAHRIPFYIAAPVSTFD
                     FSIKTGEDIPIEERQADEVTTFRGAQAAPKGMKVYNPAFDVTPARLITAMITERGVLT
                     PPFPAAIARLKRFVP"
     gene            complement(16803..18257)
                     /gene="gatB"
                     /locus_tag="PLA58_04125"
     CDS             complement(16803..18257)
                     /gene="gatB"
                     /locus_tag="PLA58_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOR62015.1"
                     /translation="MDFETVIGLEVHAQLLTDTKIFCSCSTQFGAAPNSHACPVCLGM
                     PGVLPVLNKKVVEYAMRMALATHCAINPQNVFARKNYFYPDLPKGYQISQYAYPLAEH
                     GYIMIDTGEGSRKIGITRIHMEEDAGKLIHDDHNPASYVDLNRTGVPLIEIVGEPDIR
                     SPEEAAEYLRRLHEILVYLDICDGNMEEGSFRCDANVSIRPRGQKEFGTRTELKNMNS
                     FRNVQRGLEYEVRRQQYLVENGEKIVQETRLWDDAQGVTNPMRSKEEAHDYRYFPDPD
                     LVPVVVDEAWVADIRAGMPELPQAKRERFVNDYQIPAYDAGVLTADKALAGYYEDVVK
                     LCGKPKIASNWVMGDVMKFLNEDKKSIRECPVSAKSLADMIGLIEEGTISGKIAKEVI
                     ADMYATGRTPQEIIREKGLVQITDEGALAKTVAAIIEANPGQLADYRGGKEKLFGFFV
                     GQVMKATQGKANPQMVNDLLKKMLALRQAQDDID"
     gene            complement(18257..19714)
                     /gene="gatA"
                     /locus_tag="PLA58_04130"
     CDS             complement(18257..19714)
                     /gene="gatA"
                     /locus_tag="PLA58_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOR62016.1"
                     /translation="MELHQFTIHALQEKIRNGEVSALQIAESVFSRIDAVEGRIHSYI
                     RLMKEEAMASAARADEGIQKGDIQPLTGIPIALKDIVCTRGIPTTCGSRMLHQFVPPY
                     NATAVEKLAAAGAIFVGKANMDEFAMGSSTETSYFGPTRNPWDLERIPGGSSGGSATA
                     VAADECIASIGSDTGGSIRQPAALCGVVGMKPTYGRVSRFGLIAFASSLDQIGPLTKD
                     VEDCAIMMNVLAGYDPKESTSVKAEVPDYRQFVGRDIKGWKIGIPKEYFIDGIDPEVS
                     AAVAKTIETVRQCGASIVEISLPHTKYCVAVYYIIAPAEASSNLARYDGVRYGFRAAD
                     ARDLSEMYQKTRRQGFGAEVKRRIMIGTYALSAGYYDAYYGKASQVRALIRRDFDEAF
                     ARCDVVLTPTTPTPAFKIGEKTDDPLQMYLSDIFTISANLAGIPGISVPCGFSTSGLP
                     IGVQFLAGHFEEGKLLQVASAYEKSAAVEKRRPAL"
     gene            complement(19841..20128)
                     /gene="gatC"
                     /locus_tag="PLA58_04135"
     CDS             complement(19841..20128)
                     /gene="gatC"
                     /locus_tag="PLA58_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOR62017.1"
                     /translation="MKLSTADVEYVAKLARLEVSEAEKEKLTAQINDILRYIDQLNEL
                     DTMGVAPMSHAIAVTNAFREDRAAQSLEPGAALANAPDARGEFFRVPKVIE"
     gene            complement(20138..>20487)
                     /locus_tag="PLA58_04140"
     CDS             complement(20138..>20487)
                     /locus_tag="PLA58_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOR62018.1"
                     /translation="NAFAPELILVSAGYDIYYQDPLGGMRVTPDGFAAMARVLLNIAD
                     RCCSGHVVGVLEGGYHTVGLARSAKATLEEMFDETHWTDRKLEDLEQHADEENQPVIQ
                     SVIKGMRPYWNVF"
     assembly_gap    20488..20587
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20588..21309)
                     /locus_tag="PLA58_04145"
     CDS             complement(<20588..21309)
                     /locus_tag="PLA58_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOR62019.1"
                     /translation="MQHATGMDHPESPERLAAVYEMLDNPHMSWKYESIEPREATHEE
                     IAYVHTLPYIREIAGTAGKDCVFLDSDTIAGPESYEIAKLAAGGMLNAIDAVMTGEAD
                     NAFAFVRPPGHHAGAGNSEGFCIFNNIAIGAMHAMKKHGLKKILIVDWDLHHGNGTQK
                     MFYSDRRVLYFSTHQYPYYPGTGSLLEIGEGQALGYTVNVPLRRGAVNGTFVSVFRRI
                     LEPIANAFAPELILVSAGYDIY"
     gene            complement(21453..21662)
                     /locus_tag="PLA58_04150"
     CDS             complement(21453..21662)
                     /locus_tag="PLA58_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62020.1"
                     /translation="MKKLNPESLPSSIPAGNTDPGPIVDFAHLASVMAPEATDDFLHP
                     PYGFSAADEEDLYTTGYLQRFFLRC"
     gene            complement(21737..22570)
                     /locus_tag="PLA58_04155"
     CDS             complement(21737..22570)
                     /locus_tag="PLA58_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62021.1"
                     /translation="MKKNGISKWLFAQAMVVLLLWPATALCAAGANQALEAARQKVAA
                     ELNRLDEGLREAAAALGKTGLIGQNARPVLASLCRKFDYAVDCAAVNLQGRMAVVEPE
                     VFRRFEGKDISGQEQVRRVLKTGSPVLSSVFFSVEGFPAVDAEYPVVSPDGKRIGSVS
                     VLFQPEKFLGGIFVPLVRQTSVEIWAMEEGGLILYDADASQIGLNLFTSGRYQPYAGL
                     PALGKRIAAQPQGKGVYEFTAGRSANVVRKSALWKTVSLYGTSWRLVAVTVEHPAGKA
                     K"
BASE COUNT         4983 a         6728 c         5830 g         5095 t
ORIGIN      
        1 gattgagttt ccttcctcgc acccgaccct tcacgcacct catctctttt gatccgccgc
       61 ctcatctatg gcccctcgcc tgtttttacc attcaccacg gaggttctaa agaccctccc
      121 tacgatttcc tcgtcactcg tcgtattcca cccatctctt tttgaccgat gctcgtcttc
      181 ttatggccca tggcctatta cctattcctc ctgacaccgc ggacagtaaa acgtactgcg
      241 tccaccctgc cggatccgaa cgatgatccc gccgcatcgg acacagtcct cgccttcccg
      301 tccgtaaact ttcagctcct tctggttttc ccccggacag ccgtaaaggt cccgccattc
      361 ggaaatagac gttccccgcc cggcaatggc ctttctcaga atacgccggg cctgacgaaa
      421 aatttttttc cactcggatt ctccaagcga tgccgccggc ctcagcggag agatgcccgc
      481 tgcgtgcagg atttcgcagg cgtaaatgtt gccgatgccg gcaatcgctt tcggatccat
      541 caacagtgtt ttgacgtgaa tcctgcgccc ggcctggctt tgcatgaagt tcctgaaatc
      601 aaagcggccc atcagatcat tggacaagga ctcccgaccg gcatggacca cccggacagt
      661 tgcaaagcgg cggggatcga cgagatcgag gttgccgtca accagcgtca tccgccatcg
      721 cgtatgcggc tccttcttgc ggtccgcccg ccagaacaac cgtcccgaca tgcgcagatg
      781 aacaacgatg cttctgccgt catgaaggcg catctcaacg gtctttcccc ggcggcaaac
      841 cccgcaaatc ctcatcccgc ggacattcgc gatgccgtcg agtttgtgat cgaagacttt
      901 cgccgccaga attttctttc cggcgatttt tccctgcaac tggcaacaca gcgtttcaac
      961 ttcgggcagc tccggcattc tcgatcctga ttgcgttgaa aaaaatttct ccacgcactt
     1021 tttttaaaat ttttttcatt ttttctttca aaatgcaaaa taaatgttga caaatttttt
     1081 tgcttgcgta aattcaaaaa tcaataaaag ttttttttag aaggattttt ttagcggaag
     1141 gatgcttccg gaaggaggat ggatggaaaa cgagaccctg tttaatgagg atgcagaacc
     1201 tcctggcccc tcagccggtc ttgttgtggt gccgaacaaa acaacgaatt ttttttcgtt
     1261 gaccacaaat aatcacctcc cctccctggc aaaatcaaag ccggaacggc tttactttcc
     1321 cttcaaagat tctccccgct cacgggcttt ccgaaaaaaa cattacccgg acatcagccg
     1381 catcgaatgg tttgactggc gatggcagtt tcaaaacgcc atccgccatg tccagaccct
     1441 ggacgccatg gttcaccttt ccgacaatga acgccaggcc atgatgcacc cgtcagggga
     1501 tctccctgtc gccatcaccc cttactatgc cagcctgatt tccccggaag acccgtcttc
     1561 tccgcttcgc cgctgcgtca ttccggtggt cgatgaatgc ctgcatacca agggcgaaga
     1621 agaagaccct ctcggcgagg acgcagattc tccggtcccc gggattgtgc atcgctatcc
     1681 cgaccgcgtt ctgtttctgg tcacggacat ctgttcgacc tactgccgct actgcacccg
     1741 ctcccgcatt ttcggccgcc agcaccaatg ctttatcgac acggccaagt gggaaaaagc
     1801 gctggactac atcgcctcca atccgaatgt ccgcgacgta ctcctgtccg gcggcgaccc
     1861 cctgaccctt tccgacgacc ggctggaatg gctgctttcg aggctcaagg agattcccca
     1921 tgtggaactg gtccgcattg gaacgaaagt ccccgtggtg atgccgcagc gcattacccc
     1981 ggctcttgtt tccatgctga aaaagtgcca tcccctctgg atgagcattc atgtcacgca
     2041 ccccgacgaa ctgaccccgg aaatgagcgc cgcctgcatt cgccttgccg atgccggcat
     2101 tccactgggc agccagacgg tattgctggc cggcatcaac gattccgttt ccaccatgac
     2161 ccgcctggtc cacggactcc tgcagatccg cgtccgtcct tactatctgt accagtgcga
     2221 cccgatcccg ggctcgtccc atttccgaac acccatcagc aaaggcctgg aaattatcca
     2281 gggcctgcgc ggccatacca ccggctatgc cgtgccgacc ttcgtcgttg acgcccccgg
     2341 cggcggagga aaaattccgc tgcttcccga atatgccatc gggtgggaca agggcgacct
     2401 gatgatgcgc aattacgaag gtaatatctt ccgctacccg gacaaccatg ccgagtcaaa
     2461 ggatatccac taggatattg aaatagctta tgtccggtaa ccccgtctgt ccatcgaccc
     2521 ccccttgccg aacggtcacc atcggcctga cctacgacct gcgcgacgat taccttcgcg
     2581 aaggatacag cctcgaagaa accgccgagt tcgacaagcc cgataccatc gaagccattg
     2641 aaaacgtcct gaagaaaaac ggccacgcga ccgaccgcat cggccatgtg aaggcgctca
     2701 tccgccgtct ggccgcaggc gaccgctggg accttgtctt caacatcgcc gaaggcatgc
     2761 acggttttgg ccgggaggct caggtccccg cgctgctgga cgcctaccgg atcccctaca
     2821 ccttttcaga cgccctcggc caggcgctga cgctccataa aggtatgacc aaacatattg
     2881 tgcgggacat gggcattccc acgccggact ttgccgtggt ctcctctccg gaagacatcg
     2941 gtcgcgttct gctgccttac cccctctttg ccaaacccgt cgcggaaggc acgggcaaag
     3001 gcatcaccgc ccgttccaga atcgacaacg cggaaagcct gcgctcggtc tgcgggactc
     3061 tcctgcagac tttctgccag ccggtgctgg tggaaacgta tctgcccggg cgcgaattta
     3121 ccgtcggcat catcgggacc ggcaaaaacg cccgcgtcat cggcgccatg gaggttctcc
     3181 tgaatccgga cgcggaaccg catgcctatt cctatgacaa caaagagcat tatgaaaacc
     3241 tggtgcgcta taccgccgtc tccgacccgg aagccctgcg ggccatggaa gtctccctgg
     3301 cggcctggcg cggactggac ctgaaggacg gagggcgcgt ggacctgcgc tcggacgctt
     3361 tcggcagtcc tcattttata gaggtcaatt ccctggccgg tttgaatccg gtgcgttccg
     3421 atctgccgat tttgtgcgga ctggcaggca tatcctatga tcaactgatc accgacatcc
     3481 tgaactctgc actggagcgg gcgggtcttt ccagggggca aagcctccaa cccgacggcg
     3541 ccggaacgga aaagtctcca tgaaaaaaat cctgattctg cacagcgatg tgccggcgga
     3601 tgcgggcgaa gacgagcttg attgcctgac acaggcccgt accatcggcg aagcgatcat
     3661 ttcgctgggc caccggccgg aaaagctttc cttcggaatg gatctgcagg ccaatatctc
     3721 caggatccgc gaacatcagc cggacatcgt ttttaatctg gtggaaacgc ttgccggaaa
     3781 aggcagcctg aatcatttcg cgaccgcgct tctggatttt ttacgcctgc cctacacagg
     3841 ctgccggaca gacgccatgt ttgtcacatc caacaagccg ctggccaaaa aaatgctgcg
     3901 gcaggcgggg atcgccacgc ctgactggat cacactggaa gacgaaacag aaagtccgtc
     3961 cccgggagag gtctattttc tcaaaccctg ccgggaagac gcatcggtcg gcctggatga
     4021 ccgggccgtc gtgcagatgc agaatcctca aaccatccgg gacacgctgc gggagcgcca
     4081 ggatgctctc ggtttggact gctttgccga ggcctacatt gacgggcgtg aattcaacat
     4141 tgccctgctg gcttccgatc agggggtgcg ggttctgccc cccgcggaaa ttctttttct
     4201 ggactatccg ccggataagc gcaaggtgct cgattaccgc gccaagtggg ttgaagattc
     4261 ctttgaatat tcgcataccg tccggtcgct ttccatcgca ccgcaggatg agcgcctcgc
     4321 ggaccggctg cgggacatcg ctctccggtg ctggcatctg ttcgggcttc gcgggtatgc
     4381 ccgcgtggat ttccgcgtgg atggaaacgg catcccctgg gtgctggaag tgaacgccaa
     4441 tccctgcctc tcgccggacg ccggctttgc cgccgcgctg gatttcgcgg gcatcgaata
     4501 caccggcgcc atcgacgcca tcctccggga cgccttaaaa ccataaacaa aggggaatga
     4561 ttttttgaaa acgattacct accggcagga aatccggccg accgatccgg acgcgattgc
     4621 agagattgtg cggggaagcg gtttcttttc cgccgcggaa gaagcgctgg cccgtgaact
     4681 ggccgaggaa cggcttgaac agggcgaaca gagttcgtat cggtttttat ttgctgaaga
     4741 agacggccgg gtggtcggat acacctgcta cggattgatc ccggccaccg ccggcagcta
     4801 tgacctttac tggatcgccg tgacgcaatc cctgcggggt caggaaaccg gcaaacgtct
     4861 cctgcaaaaa accgaagaga tgatccggga cctgggggga aggaacattt atgcggaaac
     4921 ctcttcccgt cctcaatacg cgccgaccag gcatttttat gaaaaatgcg gctatcttcc
     4981 ggaagcgttt ctcaaggatt tctattctcc cggagacagt aaaattattt actctaaacc
     5041 ccttatataa caggcattaa cgacttcttc gccccgcgtc cgtgcgataa aatcttgcaa
     5101 tttccccgat aattcgctag agcacgtttg gcttttgggt caccccaacc gcccatcgaa
     5161 ggaaaaaagg ggaatttttt tatgcgcctg tttccaaagg aagaaaattt ctttgagcat
     5221 tttgaagagc tggccaataa gatagaggaa ggcggtcagt tttttctcga aatgaccaga
     5281 aaccgggact attccgaggc caaagtcgcc cggctcaagg aactggagca tgaggcggac
     5341 ggcattacgc ataaaaccta cgaaagaatg cataaaacct tcctgacgcc cattgaccgg
     5401 gaagatattt acgcgctggt caataaaatg gacagcatca tggacgtcat cgaggccacc
     5461 gccgtccgca tctacatgta taaagtgaaa aaacccgatg acgaaatcat caagcaggcg
     5521 gaaattcttt ttcaggccat caaaaaaatc aagggcgtcg tccacggcct gcgcgatatg
     5581 aaaaactccc agatgattct cgacggctgc gtcgaaatca ataccctgga aaatgccgga
     5641 gatgtcgttt taagaaccat catcaccaat cttttcatca aagaacagga cgcgatcgaa
     5701 ctgatcaagt ggaaagaaat cttcgaaagg atcgaagaag ccatcgatgt ttgtgaagac
     5761 gtttccaaca tcgtggaagg gattgtgctg aaacatgctt gattccatgg catttgtttt
     5821 catcctggtg gccattgccc tgatttttga ttttctcaac ggcttccatg attccgcaaa
     5881 ttccatctct acagtggttt ccacccgcgt tttgtccccc aaatacgcgg tgctctgggc
     5941 ggcttttttc aattttgccg ccgttttttt tgtcggaacg caggtggcgc acacggtcgg
     6001 caaaggcatc atccacatcg atatcgtcga taatctcgtc attctgtccg cattgggcgg
     6061 ggcgatcctc tggaacatca cgacctggta ttacgggctg cccagcagtt cctcccacgc
     6121 cctgatcggc ggcctgatcg gcgcgggggt cacaaaagcc ggaaccggca cgctggtgtg
     6181 gagcggcatt atcaaaacct ctctgttcat catcatctcg cccgccgtcg gcatggcgct
     6241 ggggttcttc ttcatggcgc tggccatgaa tctgagccga aactccaata tcgccaagtc
     6301 cgacaaggtc ttccggaagc tccagctttt ttccgccgcg gtgtattcca tgggccacgg
     6361 catgaacgac gcccaaaaaa cgatgggcat tatcgccatg gcgctcttca gcaaggggct
     6421 cctgggggat acgtttcaca tccccatctg ggtggtcatc atctgctaca ccatgatttc
     6481 tctgggcacc atgttcggcg gctggcggat cgtgaagacc atgggaacca aaattaccaa
     6541 acttcagccc atcggcggct tcagcgcgga aaccgcggcc gccttctcca ttatcggcgc
     6601 gacgatgggc ggcattcccg tgagcacaac gcacaccatc accggtgcga tcgtcggcgt
     6661 gggctccacc aagagactgt ccgccgtacg ctggggcgtt gcgggaaaca tcatctgggc
     6721 gtggattctg accattccca tttccgccgt gattgcctcc gtgatttatc tggccgtcaa
     6781 cagctttgtc tagccgaacc ttccccccgg ccgattgaaa atgccattgc caatttgttc
     6841 attttgccgt aaatgactca tatcgaaccg aacacagggg atttgccgat gccggaaaaa
     6901 cgactgcctt ttacgcggaa acaactgtct gccatccttg aaaaatatcc gacacccttt
     6961 tacatctatg acgaaaaggc ccttcggaaa agcgcccgcg cctttgccaa ggccttttcc
     7021 tggaaccggg gctttaaaga attcttcgcc gtcaaagcct gccccaatcc ctacatcatg
     7081 aaaatcctcc atacggaagg attcggagca gactgcagct ccatggcaga attgctgctg
     7141 gcgcaaaaag tcggcatcac cggggaagaa atcatgtttt cctccaatga cacccccgcc
     7201 gccgaatttg tccaggcccg gaaactcaag gcaaccatca acctggacga cctatcgcac
     7261 attgattttc tggaaaaaca cgccggactt ccggacctga tctgctgccg ttacaatccc
     7321 ggccccctga aaaaagggaa catcatcatc ggccatccgg aagaagccaa atacggattc
     7381 acgcgcgaac aactttttga aggctaccgc atctgccggg acaaaggcgt ccggcgcttc
     7441 ggcattcaca ccatggtcgc ctccaacgag ctggatccgg attactttgt cgaaacggcg
     7501 gaaattcttt ttcatttgat cgtcgaatta tcccacgccc tgaacatccg ctttgaattc
     7561 gcaaacttga gcggaggatt cggaattccc taccggcccg aacagaaagc catcgatctg
     7621 gaggttatga cccgcggcat ccgggaaaag tatgaaaaga tcatcacagc cggcggcatg
     7681 gcccccctga aaatttttac ggaaagcgga cgcttcatca ccggccctca cggctacctt
     7741 gtcgcgaaag tcctgcacat caaggacacg tacaaaaaat tcgccggcct cgatgcctgc
     7801 atggccaatc tgatgcggcc cgcactttac ggcgcctacc atcacatcac cgtcgtcggc
     7861 aaggaaaggc gcccccacga catcctgtat gatgttaccg gctctctttg cgaaaacaac
     7921 gacaaattcg ccatcgatcg cctgctgccc cgactggccc cgggcgacac cgttgtgatt
     7981 cacgacacgg gcgcgcacgg ccattccatg ggctttaatt acaacggcaa gctgcgctcc
     8041 gccgaactgc tccttcggga ggacgggtcc gtcatccaga tccgccgcgc cgaaaccgtg
     8101 aaagattatt tcgcgacgct ggacttcaag aggttgagca ggtttaaagg ctgaagactg
     8161 aaggactatt ttgtgggcct tcggcctttt ttatctatac tggatgccgg cctccgccgg
     8221 catgacggcg catttagcgc ctttccgttc aaccttcaac cttcagtctt atgccttctc
     8281 cctgtatccc tttatccttg ccttttcgca tccccgtcat tatattgccg gtatggcaaa
     8341 ggaaaaaatc ccatcaacgc ccgccgtcct ggttctgaaa aaatcggcgg cgacttttac
     8401 actgcagacc tatgcctacg aagagcacgg cggcaccaag gtttcttcgc gaaagctggg
     8461 cgtggatgag cattgcgtga ttaagacgct ggttatggaa gatgaaaatg cgaatccctt
     8521 catgattctg atgcacgggg accgggaggt ttccacaaag gctctggccc gcgcgctggg
     8581 cgtaaaaacc gtcagaccct gcacaccgga gaccgctcac cggcacaccg gttattttgt
     8641 cggcggaacg tctcccttcg gcgccaaaaa ggatttgaag ctttacatgg aaaagacgat
     8701 ttccggcctt ccggaaattt tcatcaatgc cggctcgcgc ggacttctgg ccaggatgcc
     8761 gccttcggaa ctggttcgca tcctcaaccc ctttgaagtc agcgttgcga tataatttgc
     8821 catttgaacg gaaaaaagag ataatgccgc aaaatttcgg gagatgaaga atgccggaaa
     8881 acgttattat ccgctgtctg aactgcggaa caaaaaaccg cataccgcag caaaaatttc
     8941 agcgccgacc tacctgcggg cattgccacg cgccgctgga tcaactgatt atccgctgcc
     9001 tgaaatgcgg caccaaaaat cgtctgcccg aagagcgcct cagctcaaaa cccttttgcg
     9061 gcaaatgcgg cgaagcgctg gttgttgcca cgcaaaacat ccaacccgtc gaggtcaccg
     9121 atgactcctt tgcccgggaa gtcctgtccg cggaaatctc cgtgctggtg gactgctggg
     9181 cgccctggtg cggcccctgc aaaagcctgg cgccgaccat tgacgagctg gcctcggatt
     9241 acgccaacgg cgtcaaggtc gccaaattga acgtggacga aaacccggca accgccactc
     9301 agtacggcat ccgcagcatt cccacgctgc tgtttttccg ggacggccgg ctggtcgaac
     9361 gcctcgtcgg cgccctgccg aagcaggaaa tcgaacggca tcttctttcc atcatcaaaa
     9421 ccaactgaaa cggaaaccga tacccttcat ggacaaaaac aggactctgg cgtacgcgtg
     9481 taaaatcttc tctgtctggg aagaagacgt tctgctgcaa aacgggaaaa aaacccggca
     9541 aagctggatt gaacacaaac ccaccgtggc cgtggtcccc gtcaacgatc aaaaagaact
     9601 gctcttgatc cggcagttcc gcatccccac cgggaaaaac ctgctggaaa ttcccgccgg
     9661 ttccatggac cacggagagg aatcaccggc ctcctgcgcg ggacgtgaac tggccgaaga
     9721 aacaggctgg cgggctaaaa agctgacgcc ccttttcgcg ggctacctgc ttcccggata
     9781 ctgcaacgaa ttcatgtatt tttttctggc gcaggacctg gtgtacgaac cgctgcaacc
     9841 ggatgaagac gaatgcattg aagtcattcc cgtgagtttc gacagggccg ccgccctttt
     9901 gaaaagcggg gagctgaacg acgcgaaaac cgccctggga atcatgctgg ctgaacgata
     9961 tttaaagctc tgatgtcagc gcagcagctt ctccctcttc tccagccgaa tctccaaatt
    10021 ctttaactgc cggatatcat ttttcagctg ctcgttttcc tgctgcagac ggatcagctc
    10081 cgccgaattc ttttcttccg cctggcggac gctgcttttc agtccttcaa tttctttttt
    10141 gagtttcgcc tgttcctgct gggcttccgc cttctgttct ttcaactgaa cctgcagcgc
    10201 ggaaactctg tccagcattg acagcagagc cttcgcgccc tccgcccatt tgctttcagg
    10261 aaattgctcc accaaagttc ccagacggat tcttgcctca tggtaatccg gctcccgccc
    10321 gctgccggac aacagggcca gtgcttcatc gaacagatcc cggtccgacg gcccgctgct
    10381 gaaaaggctg ccggctttct tcaccggcgc tggggactcc tggcctccat cccggacggc
    10441 ctttgccgaa cagccgcaca acatcagaag cagcaggatc aaaaaaacca gttttttcga
    10501 atgtctcaac aattgctccc ctccaggctg ctcggtaaat tttgcggctg cgccgccttt
    10561 tgtctttcca ccgcttcttc cgttacataa ttgctgccgg gcattctttt ggcgtactct
    10621 ttcaactccg ccacccgtct ggccatctcc agcgggtttt taaagcgcga tccgtcatcc
    10681 gtaacaatcg ccgcggtgat cgtgatcaat ggaaatttct gaacggttcc ctggcgattc
    10741 cgtccgatga aatatccctt ttgcgcatct tccggccggt agtagcgaag gatgcgtccc
    10801 gcaaaatcca ccaccagctt tttgcagatg ccctccacct gtccgggatc gccgatgaga
    10861 acaaaatcat cgcccccgat gtggcccaga aacatgtcgg tcagcttcat ttcatcaata
    10921 tacccttgga gaagaccggc gacttcttta atgacttcgc ttccccacgc gtagccgtac
    10981 tgatcggcaa aaggcttgaa attgtcgagg tccacctggc acagagaaaa aagcttcccc
    11041 tttgccagcg actgttcaat ccggttttca atcgccagat ttcccggcag cccggtcagc
    11101 ggactggcgt cgaggttgag cgcttccagc actttgagcc tttgggtcat gtgggcaaaa
    11161 gaatcggcca gtgctccaat ttcatcgttt cgtcttgcct cgattttgtg atccagattc
    11221 ccttcggcaa tcaggcgagt ggctttctgc aattgtttca atggtttgga aatattgcgc
    11281 gtaatgatca gcgccagcgt tatgcccagc accaggctca gcaaactcag ccccagggtc
    11341 atctgaacgg ccgcccgtcc ctgactggcg atccggttca tttcattatt gatggcctgg
    11401 tccatgtttt tttgaatgtc ccggagctgc atcgccatgg catcgatcac agccctccct
    11461 gttgtgtcgg aaatggcctg cgcttcctgc aagtgcccgt tttgcgccag cagggtttct
    11521 tccgaaaaca attctttcag gcggcggtgc aacacgtcca cttcggcaac cgcgcttttc
    11581 gtgcgacggt caagtttcag acggcttgtc tcgtccagtt tttccgtaaa ttcaacgctg
    11641 cgcttccaga aaatctgctc cagttcaggg tctctcagaa tcaaatattt cttttcggcg
    11701 ctttcctgag ccagcagaat gtccatgagc tgtttggaat tttccatcag cacaaagtga
    11761 tgattcgtga tattgtaggc gcctgcgctg agtttctgca gattgaacaa cgcaaaggcg
    11821 ctggccagga cggtcaaaag cgccataaac agatacccca gaaataattt gcggctgatc
    11881 gtgagactca tcttttccaa aacccgtgca gacggatggt cctgtggaat gaaatgccat
    11941 tccccatcac tgccttttgc acgtaacaca aacaaccggt ttccgtaaag aataatttgt
    12001 ttttcgggct gttttgccct tttttcaccg gctcccgcac tcccctgaaa gctgcttgcc
    12061 accggcggaa agttttgtta cactgccgca caaaacaggc agccccttac gtcagctcag
    12121 caaaaccgcg gagacagcct tcggcttggt cctgaaccgc ctgtcagaaa ggaagacgcc
    12181 gccatgatgt acaatgatga gtttgaaacc ctgccgcgtg aagctctgaa agccctgcaa
    12241 atcaaaagac tccagcaggt tctgcagcgc gtttgcgcaa cggtctgttt ttacaaaaag
    12301 tcgtttgccg ccgccaaaat atcgccggac gccatccgct ctctggagga cttaaaaaag
    12361 cttcccttca ccacccgcga ggatttgcgc aacaactatc ccttcggcct ttttgccgtt
    12421 ccgatgagca acatcgtgag gctgcacgcc tcctccggaa cctccggccg gtcctccgtg
    12481 ttcagctaca caaggcgcga cctggacacc tggacggatc tgaccgcccg ctccctggcg
    12541 gccgcggggt tgaccaaaaa cgacatggtc cacaacgcgt ttggctacgg tctgctcccc
    12601 ggcggcctcg ggctgcacta cggcatcgaa aaaatcggcg ccagcgtcat ccccatctcg
    12661 gacggcaaca ccaagcggca aatcacgatc ctccaggatt tcggcccgac ggtgctgtgc
    12721 gccacgccat ccttcgccct gcatctggcc gaggaaggaa gggccatggg cgtggatatg
    12781 caatccctgc aactgcgggt gggcatcttc ggcgccgagc tctggagcaa cgccacccgc
    12841 gacgccattg aagacgcctt caacatcaaa gcgctcaaca tcttcggcct ttcggaaatg
    12901 atggggccgg gcattgccat ggagtgtctg gaaggccggc gcggaatgca tatctatgaa
    12961 gatcatttta tcgttgaaac catccatccg gaaaccggcg acgtcctgcc cgaaggggaa
    13021 gagggagaac tggtctttac cagcctgacg agagaagctt tcccgctggt ccgctaccgt
    13081 tcgggcgaga tctcccgcct gattcccgaa ccctgccgct gcggccgcac gcacattcgg
    13141 atggagcgcg tgctgaaaag gagcgacgac atgctgacca tccgcggcat caatatcttc
    13201 cccgcgcagg tggaagccat cctgaaggcg attgacggcg tcgagccgga ttatcagttg
    13261 atcatcgaca aagtgggcgc gctggatgcc gtcgaactgc acgtggaagt cggcgaaaaa
    13321 ttcttcggcg aagcgggcgg cgtcaaggag ctgcagacta ttgaaaaacg catcgtcaag
    13381 gacatgaaag attacctgag catttcgccg cgcgtgaagc tggtggcccc gcagacgctg
    13441 aaagaaagcg gaaagaaggt aatcgacagg cgcaccatct gataaagtat cggttcatcc
    13501 ctttgcaggg aacggtcgcg accgttcccg accgccacga acttaaaaac tatggaggaa
    13561 ttatacatga aggtagaaca aatctcggtt tttctggaaa acaaacccgg cacgctggag
    13621 cacgccacgc gcatcctgaa ggaaaacaac atcaacatcc gcaccctgtc gctcgcggaa
    13681 acggcggatt tcggcatttt gcgcctgatc gtgaatgacg tggagaaaac caacaaggtt
    13741 ctcagggaag ccggtttccg cgtcagcaaa accgtcgtcg tcgccgtgga agtccccgat
    13801 cagccgggcg ggctccacag catcatggaa gttttgaacc gggaaaccat caacgtggaa
    13861 tacctgtacg ccttcgtgga aaaaagcggc ctgaacgccg tcatcatctt ccgcttcgac
    13921 gctcccgaaa aagccatcga ggtgcttttg aaacacaaat tcaccgtcgt gccgggatca
    13981 aaactttatg agttctaatg aaatggcatg gaatgactac tttggaacct aaatcgggag
    14041 agcgtgtccg ggatgtgcat atcaatgatg ataccatcag tgtcgatctt ttcgatgggc
    14101 gaaccataac ggtgccattg gcatggttcc cccggctgct gcatgccaca ccggcccaac
    14161 gggcccaatg gcatgtttcg ggcgccggat atggaataca ctggccgcgc cttgatgagg
    14221 atcttacaac gcaaggactt ttacagggcg ctcccgcccc tcggaaaaaa gaaaaagccg
    14281 cttagctccg cccgcgaagt gtaagtgtag atggactttt ttaaggggcc gtcgccgttt
    14341 gcaaagccag caatcgcgct ccatggatga cccgcaggat aatgacctga gtatcgatca
    14401 ttttaaagat cgttcgataa ttgccaaaaa gcagatgacg atagcttcgg tccaggaatt
    14461 cattttccgg aatgagcgga caacgttcag gatattgttc cagcgaagca atttgttctt
    14521 caagctgaag gataaaagag gttgcggcgt cagggttgtc ctgggcaatg taatcccata
    14581 attcagcaat atccgcctcg gccgtggcgg taatatcaac tttatatttc tttttcacgc
    14641 ttgaattcct tcaaaaactc ctgcgcggaa cggatttttc ccttttccac gtcctgttcg
    14701 gcaggcgcta tgagttttgc aaggttcact gctcgcaggt ttttttcata ttgcctgacg
    14761 tcgagcagaa ccgaatcagc tctgccgttg accgtcagga tcacgggcct ccccgtggca
    14821 tgtaaatgat tcaaaatttc acgagtattg cgctttagtt ccgttacgga acgaatatct
    14881 tccgaagggc ttatttgtgt catctttaat ctccttttta gtgctctatt gtgtgccata
    14941 aatagcatta tttctcttgc cttacaagaa ataagcttaa gcttaagggg attagactga
    15001 agactgaagg ttgaagacaa aaatttcagt tgagttgcag cagatcttcc ggccggtcca
    15061 gcaggtaatc gggaccggtc gcggccagtt tttcccggga atgccagccc caggtcaccg
    15121 ccacggtttt gacgccggcc tgcttgcctt ctttcatatc gccggtcgtg tcgccgatgt
    15181 aataaatatc ctgcagggtg acgttgtatt tctgaatgac gtgaaggatc ttgtcctttt
    15241 tgctcagcat gaaatcgcac cccagaatat cgtcaaaaat cccgttgaaa tcatacaggc
    15301 gcagggcttc tttgatcgtc ggcgcgtcat tggatgagat gatgatcagg ggatggtttt
    15361 tcttcagttc ctgcagcacc ggccggattg cgtgaaaagg cttcatgtcg gcgtaacgga
    15421 cttttgccag aatgtccacg gacgcgtccg aaaacttttt catgtccacg cccttgtctt
    15481 cgagcgactg gtaaaagttg ccctcaaaaa gctccaggaa ctcttcccga ccgcgcgcga
    15541 gcggccggcc gatcgcgctg aggcaatccg tcaccgtttt ttcataaacg tccagcgaat
    15601 ccactaaaac gccgtcaaaa tcaaaaagca gcagtttcat attcctgtat tcctgtcttg
    15661 cagcaaaacc taaatctggt cattctgcgc atgatctgaa atccaggaaa gtttgagaaa
    15721 atctggattc ccgcctgcgc gggaatgacg caatacttat gattcatcat ggaacaaatc
    15781 gcttcaggcg ggcgatggcc gccgggaagg gcggcgtcag gacgcctctt tccgtgatca
    15841 tcgccgtaat cagccgcgcc ggcgttacat caaacgccgg attgtacact ttcatcccct
    15901 tcggggcggc ctgcgcgccc cgaaacgtcg tcacttcgtc cgcctgtctc tcttcaatgg
    15961 gaatgtcctc tcccgttttg atcgaaaaat caaacgtgga aaccggggcg gcaatataga
    16021 aaggaatccg gtgagcccgc gccagaacgg ccagcgcata ggtgccgatt ttattggccg
    16081 cgtcgccgtt ggccgcgatg cggtcggcgc cgacaatgac cttgttgatt tttccctgac
    16141 gcatcagaga tcccgccatg ttgtcgcaaa tcagcgtggc gggaattttt tccttcttca
    16201 tttcccaggc ggtaagcctt gccccctgca gaaccggacg cgtttcatcc acatacacat
    16261 gcagcttctt tccttcggcc gcggccgcgc ggagcacacc cagcgcagtg ccgtagccgg
    16321 ccgtcgccag cgcgcccgcg ttgcagtgcg tgaggacatg gtccccgtcg gagaaaagcg
    16381 ccgcgccgcg gcggcccatc tcccggttga cggcaatgtc ctcggcgcag atgcgcttcg
    16441 cctcccggac cagctcgcgg gccggatttc tctttcccga acggacgact tcgtccagat
    16501 gctttttcat ccgctccatt gcccagaaaa gattgcgggc cgtgggcctg gcggccgcga
    16561 tttcatcgca gatcgcgttg aatttttttt tgaatttttc gatgggttcc gaagcaaacg
    16621 caagcgctcc cagcgccgcg cccatggcgg cggccacgcc gatggccggc gcgccgcgca
    16681 ccgtcaaatc ccggatggcg gcaatcacct gccggtagtc ggagcagacc acctgccgct
    16741 ccacaagagg cagagcgttc tggtcgatca ggaccactgc gttttttttc cagaaaaccg
    16801 ttttaatcaa tgtcatcctg agcctgtcga agggccagca ttttcttgag caggtcattc
    16861 accatctgcg ggttggcttt tccctgggtc gccttcatca cctggccgac gaaaaacccg
    16921 aagagctttt ccttgccgcc gcggtagtcc gcgagctgtc cgggattggc ctcgatgatc
    16981 gctgcaaccg tcttcgccaa ggctccttca tcggtaatct gcaccaggcc tttttcccga
    17041 atgatctcct gcggcgttct gccggtcgcg tacatgtcgg caatcacttc cttggcgatt
    17101 ttgccgctga tggtcccttc ttctatgagg ccgatcatgt cggccaggga ttttgccgat
    17161 acggggcact cccggatgct tttcttgtct tcattgagga acttcatgac atcgcccatg
    17221 acccagttgc tggcgatttt cggcttgccg cacaatttca cgacatcttc ataataaccg
    17281 gccagcgctt tgtcggcggt cagcacgcct gcgtcgtagg ccgggatttg atagtcgttc
    17341 acaaaccgtt ctcgcttggc ctgcggcaac tccggcatac cggcgcgaat gtcggccacc
    17401 caggcttcat ccacaaccac cggcaccagg tcgggatccg ggaaatagcg gtagtcgtgc
    17461 gcttcttcct tgctgcgcat cgggttcgtc acgccctgcg cgtcgtccca caggcgggtc
    17521 tcctgcacaa ttttttcgcc gttttccacc agatactgct ggcggcggac ttcatactcc
    17581 agcccgcgct gcacgttgcg gaaagagttc atgtttttga gctccgtccg ggtgccgaac
    17641 tccttctgcc cgcggggccg gatggacacg ttggcgtcgc agcggaagga accctcctcc
    17701 atgttgccgt cgcagatatc gagatacacc aggatttcat gcaggcgacg gaggtattcg
    17761 gcagcctctt ccggcgagcg gatgtccggt tcgccgacga tttcaatcag cggcacgccg
    17821 gtgcggttca aatccacgta gcttgccggg ttatggtcgt catggatcag tttgcccgcg
    17881 tcctcctcca tgtgaatccg ggtgatgccg atctttcgtg atccttcgcc ggtgtcgatc
    17941 atgatgtaac cgtgctccgc caggggatag gcatactgcg aaatctggta gcccttcggc
    18001 aggtcgggat aaaaataatt ctttcgcgca aagacgtttt ggggattgat ggcgcagtgc
    18061 gtcgccagcg ccatcctcat ggcgtactcc acgacttttt tattgagcac cggcaaaacg
    18121 cccggcatgc ccagacaaac cgggcaggcg tggctgttgg gcgccgcgcc gaattgagtg
    18181 gagcagctgc aaaaaatttt cgtgtccgtt aaaagctggg cgtgaacttc caacccgatg
    18241 accgtttcaa aatccattat aaagcaggcc tccttttttc cacggcggcg cttttttcat
    18301 acgccgaagc cacttgcagc agcttcccct cctcaaaatg acccgccagg aactggacgc
    18361 cgatcggcag cccggatgtg ctgaagccgc acggcacgga gatgccggga atccccgcca
    18421 gattggcaga tatggtaaat atatccgata gatacatctg cagcggatcg tctgttttct
    18481 cgccgatttt aaaagcgggc gtcggcgtcg tgggcgtgag gacgacatcg catcgggcaa
    18541 aagcctcgtc gaaatcccgg cggatcagcg cccgcacctg cgaggctttg ccgtagtaag
    18601 catcgtagta tccggcggac agagcgtagg ttccaatcat gatgcggcgc ttgacctccg
    18661 cgccgaatcc ctgccttctg gtcttctgat acatctcgga caggtcccgg gcatcggccg
    18721 cccggaaacc gtaccggacg ccgtcgtagc gcgccaggtt ggaggaggcc tccgccggcg
    18781 cgatgatgta atacacggcc acacagtact tcgtgtgcgg gagcgaaatc tccacaatgg
    18841 aagcgccgca ctgccggacc gtttcaatcg tcttggcgac ggcggcggaa acttccgggt
    18901 caatgccatc gatgaaatat tctttgggaa tgccgatttt ccaccctttg atatcgcggc
    18961 cgacaaactg ccggtaatcg gggacttccg ctttgaccga ggtggattct ttcgggtcgt
    19021 aaccggccag gacattcatc atgatcgcgc agtcttccac atccttggtc aagggcccga
    19081 tctgatcgag cgaagaggca aaggcaatca ggccgaaacg cgacacccgg ccgtacgtcg
    19141 gtttcattcc gacgacaccg caaagcgccg ccggctggcg gatcgagccg cccgtatcgg
    19201 agccgatgga ggcgatgcat tcgtcggcgg ccaccgccgt ggccgagccg ccgctggaac
    19261 cgcccggaat gcgttccagg tcccagggat tgcgcgtggg accgaagtac gaggtttccg
    19321 tggaggaccc catggcgaat tcgtccatgt tggccttgcc gacaaagatt gcgcctgccg
    19381 cagccagctt ttccaccgcc gtcgcattgt aaggcggcac aaattgatgc agcatgcgcg
    19441 agccgcacgt ggttggaatg ccgcgggtgc agacaatgtc cttgagggcg atggggatgc
    19501 cggttaaagg ctgaatatcc cctttttgaa tgccctcatc cgcccgggcg gcggaagcca
    19561 ttgcctcttc cttcatcaga cggatgtagg aatggatacg gccttccacg gcatcgatgc
    19621 gcgaaaaaac ggattcagca atctgcaggg ccgaaacctc gccgtttcga attttttcct
    19681 gcagcgcatg aatggtgaac tgatgtaact ccataaacgc ctcaacaaaa tgggggttga
    19741 cacaattgtg ttctttttac ggctcgacgg cacgtttgtg tgaaatttta tccggaaacc
    19801 ggcttaaacc gccgcgcggg cggcgctttc tccgcacgcg tcattcaata accttgggaa
    19861 cccggaaaaa ttcgccgcgc gcatccggcg cattggccag cgcagcgcct ggctcgaggg
    19921 attgagccgc ccggtcttcg cgaaacgcat tggtcacggc aatcgcgtgg ctcatcggcg
    19981 ccacccccat cgtgtccagc tcgttgagct gatcgatgta gcgcaaaatg tcgttgatct
    20041 gggcggtcaa cttttccttt tccgcctccg atacttccag tcgcgccaat ttggcgacgt
    20101 attcgacgtc cgctgtgctg agcttcaaaa tctcctccta aaacacattc cagtagggcc
    20161 gcatgccctt gatcaccgat tgaatgaccg gttggttttc ctcgtcggca tgctgctcca
    20221 aatcttccag tttccggtcc gtccaatgcg tctcgtcaaa catctcttcc agtgtcgcct
    20281 ttgccgaacg ggccagcccc acggtatgat aaccgccttc cagcacgccg accacatgtc
    20341 ccgaacagca ccggtcggca atattgagca atacgcgtgc cattgccgca aacccatcgg
    20401 gggtgacgcg catcccgccc agcggatcct gatagtaaat gtcgtagccc gcggagacca
    20461 gaatcaactc cggcgcaaag gcattggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20581 nnnnnnntag taaatgtcgt agcccgcgga gaccagaatc aactccggcg caaaggcatt
    20641 ggcaatcggc tccagaatcc tgcgaaacac cgagacgaac gtcccgttga ccgctccacg
    20701 ccggagagga acattcactg tatagcccag cgcctggccc tcgccgattt ccagcaggct
    20761 tcccgtaccc ggataatagg gatattgatg cgtggaaaaa tataaaaccc gccggtcgga
    20821 ataaaacatt ttctgtgtcc cgttgccgtg atgcaggtcc caatcgacaa tcaggatttt
    20881 tttcaagccg tgctttttca tggcgtgcat cgcgccgatg gcaatattat taaaaataca
    20941 gaagccttcg gaatttccgg cgcccgcgtg atggccggga gggcgcacaa acgcaaatgc
    21001 gttgtccgcc tccccggtca tgacggcgtc gatggcgttg agcatgccgc ctgcggccag
    21061 tttggcgatt tcataacttt ccgggccggc aatggtatcg gaatcgagaa aaacgcagtc
    21121 cttcccggcg gtgccggcaa tctcccgaat gtaaggcagc gtatggacat acgcgatttc
    21181 ttcgtgagtg gcctcgcggg gttcaatgct ttcatatttc caggacatgt gcgggttgtc
    21241 cagcatctca taaacagccg ccagccgctc ggggctttcc gggtgatcca tgccggttgc
    21301 atgctgcaag tatcggccat ccttaacaat tccagttctc cgggacacgg tcaccttcct
    21361 tcttgaagca tggacggtcg tgtatcatat atttttttca tgagcaacct ccttgcggat
    21421 tcccgtggca acaggagggg ccgatatccg gatcagcacc ttaaaaaaaa ccgctgcagg
    21481 taaccggtgg tgtacagatc ttcttcatcc gcggcggaaa aaccgtaagg cggatgcaga
    21541 aagtcgtcgg tcgcttcggg ggccatgacc gaagccagat gcgcaaagtc aacgatcggg
    21601 cccggatcgg tgtttccggc cggaatggac gagggtaaag actccggatt caattttttc
    21661 atggggtgga tatatcagaa gatgggaaga atcgtcaaag attaaccggg ggaaaaacgc
    21721 gcggcagcca cgggtcctat ttcgcctttc cagccggatg ctccaccgtg acggccacta
    21781 aacgccacga cgtaccgtaa agggacacgg ttttccacaa ggcgctcttc cgtacaacat
    21841 ttgcagaccg gccggccgtg aattcgtaaa ccccttttcc ctgcggctgc gcggcaattc
    21901 tttttcccag tgccggaagc ccggcgtaag gctggtagcg cccggaagta aacaggttca
    21961 atccgatctg tgacgcatcc gcgtcataca aaatcaaacc gccttcttcc atggcccata
    22021 tctcgacgga agtttgccgc accagaggaa cgaaaatgcc gccgagaaac ttctccggct
    22081 gaaaaagaac gctcacggaa ccgatccttt tcccatccgg actcacgacc gggtattccg
    22141 catccaccgc cggaaaccct tccaccgaaa aaaagacgct gctcaatacc ggacttcccg
    22201 tttttaaaac tcttcgtacc tgttcctgtc cggaaatatc tttcccctcg aaccgccgga
    22261 agacttccgg ttcgaccacc gccatacggc cctgaagatt tacggccgcg cagtccaccg
    22321 catagtcgaa cttccggcac agcgaagcaa gaaccggcct cgcattctgg ccgataagtc
    22381 cggttttccc cagcgccgcg gccgcctcgc gaaggccttc gtccaaccgg ttcagttcag
    22441 cggcaacctt ctgccgggcc gcttccaatg cctgattcgc accggccgcg caaagagcgg
    22501 ttgccggcca cagcagcagc acaaccatcg cctgtgcaaa cagccatttt gatattccgt
    22561 tctttttcat cacttccctc atcctcaaat cgttcgttga atcgttcgtt caatgcaccc
    22621 gtgtcattga accggtaata tttgcggttc atccggttga tgtttacttt tggcaatcag
    22681 tgggttattg accaacgctt tcactactaa cagacggcga tcactttcca aataaa
//
LOCUS       DAOSRM010000026        22035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2545_length_22035_cov_105.832257, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000026 DAOSRM010000000
VERSION     DAOSRM010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 22035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22035
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2545_length_22035_cov_105.832257"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /gene="kdsA"
                     /locus_tag="PLA58_04160"
     CDS             complement(<1..807)
                     /gene="kdsA"
                     /locus_tag="PLA58_04160"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOR62022.1"
                     /translation="MKQVKLNSSTIGGGAGFILMAGPCVLENETSAMKIAAYLKKLTD
                     KLEIPFIFKASYDKANRTSIAAYRGPGVRAGLKILKNIREKIGAPVLSDVHRFEEIDE
                     AAGVLDVMQVPAFLCRQTDFVVEVAKKAKIVNVKKGQFLAPWDVANIIGKIQSAGNEN
                     ILITERGASFGYNNLVFDVRALPIMRAMGYPVIFDATHSVQLPGGMGTSSGGQRDMVP
                     YLARAAVAAGVDGIFMEVHPDPEKALCDGANSLYLHSLEELLTTLKRIDEI"
     gene            complement(804..2801)
                     /locus_tag="PLA58_04165"
     CDS             complement(804..2801)
                     /locus_tag="PLA58_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR62023.1"
                     /translation="MRKSTGYKIGFGKKIPAIAAAGTAALVLFLLLAFYPQAFRTAGF
                     YVQRGINAFAYYVLGDRPHFYCLVMEKNASDFRVKAGETLDVTYRDEFVVKSVASDDL
                     SGKSVTVFLEGAGSQRNAFGVLFRGIDLVDNLLTGGEIPEGTETAGVYQIVVYAARDK
                     IAVVPVRVVLTPQDWLRFAKNRGNSGEQEEYLKKVAARNREDAGVRKILAGIYLQQNR
                     IGEAAKMYEEILRISPGDVPAMKELARVSVRLGQDDRAIDLLTRLTGIQAQDAEAQAM
                     LGAAYGRKNLWDQAVESYARAVKMDPENYPVRLLLAQAFEKSHRNDAAVEQYRFIISH
                     SPDAAPAWLAMGDMHLRSGQYAQAIESYTRVLAIHPKNAAAHANLATAYAAQGKAKEE
                     MEHLQKAAALSPDEPVIRFNLAAAYEKRQRTSEAIREYTRVLKSNPDDADALARLADL
                     TFQSKQYDQAVRYYEKLSRIHPQQASVFARLGFARGELKQYAASAEDYEKALRLGAKD
                     QTLYYNLAFTYTKLGREKDAVRAYEKISPQNKKILSILADHYLREKKYAQAIKNYEKI
                     SKLEPKKASSWAALGYAWLASGNPDKAIENYLQALKYDREDDEIYANLGIAYEKKGLY
                     PEALKAYRSAYEINPETRVASRIPRLRIQLLHKKDDKGSES"
     gene            complement(2806..3693)
                     /gene="xerD"
                     /locus_tag="PLA58_04170"
     CDS             complement(2806..3693)
                     /gene="xerD"
                     /locus_tag="PLA58_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOR62024.1"
                     /translation="MRDLLNRYHRYLLIEKGASGNTLEAYGRDLKRYLTFLEQKEISD
                     ARSVAPQTVVDFLVQIKSEGLSANSMNRALAALRGFYNYLLQERVLEESPLTHIELAK
                     VWMRLPDTVSREEMNRILSGPDHSTPSGLRDRAMLELLYATGLRVTELISLSMNSINW
                     QVGFLAVMGKGGKERVVPVGRTAYDFVRRYVDEARPKFVRSKTTEVLFLNRFGRAFTR
                     QGLWKIIVGYAQKAGLQKNVHPHTFRHSFASHLLEGGADLRAVQVMLGHSDISTTQIY
                     THVTRERLKEIHRKYHPRG"
     gene            3797..4243
                     /gene="rpiB"
                     /locus_tag="PLA58_04175"
     CDS             3797..4243
                     /gene="rpiB"
                     /locus_tag="PLA58_04175"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOR62025.1"
                     /translation="MKKIVIASDHGAVDLKERIVAFLQKKNYEVTDLGTHGCESVDYP
                     DIAAAACREFLKGGYEFGILLCGTGIGVSIAANKIAGIRCALVCDLFSAEMARAHNNA
                     NFIAFGGRIRYAATVEEMIGKFLDTTFEGGRHERRIAKLTALENSR"
     gene            complement(4283..5098)
                     /locus_tag="PLA58_04180"
     CDS             complement(4283..5098)
                     /locus_tag="PLA58_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOR62026.1"
                     /translation="MKKIVCLFTILLLAAGICCVAQAEDSLQNIRKSGVLRAGVKDLT
                     PSFALADIKTGEISGYDVDFVRAIARRLGVRLEITPFSGEENERIDALTEGRVDIVAA
                     TMTKTKEREKLIDFSHTYFVTAQKFLVHKGTVQRLSDLKNKKIGTVDQTTSVTNLRKS
                     IPSASIVLFTGYVPAFLALQNREIFAVSTDEAILAGILTKAEKGVFEIPKVSISREPY
                     GLGVRKGHTSFLRYVNQTLLEMERSGEAQRIFDKWFGPESEAPLERTFRITAK"
     gene            complement(5127..6026)
                     /locus_tag="PLA58_04185"
     CDS             complement(5127..6026)
                     /locus_tag="PLA58_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62027.1"
                     /translation="MDDCRVQLANLVDTRDPKAVRDEIHTIAGLMFPRFDVAPFDRAY
                     SDVIALFRGNYPSYRSCNVRYHDLSHTLSVTLAVARLMHGAVEAGQSLSEKDLNMGFI
                     SGLMHDTGYIQQTSDREGTGAKYTQVHISRSIAFVQDYYRNDPAFQNDLDSFRDILLC
                     TGLNTQVKSIRFGGATVELLGKILGTADLLGQMADRYYLEKLPDLFDEFAEAGITTFA
                     GPLDLLDKTQGFYQMTLKRFAEELDNTLRFSRFHFRKRWGIDEDLYIKSIEKNMAYLA
                     RVVTLHRENYTDHLRRFARMTKE"
     gene            6353..7624
                     /locus_tag="PLA58_04190"
     CDS             6353..7624
                     /locus_tag="PLA58_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR62028.1"
                     /translation="MKKKIPDKILLSKPEKKMADLEKADEELRNLEETYRLSFEQVSD
                     VIFILDTNLRILSMSPSVETNLGYHPVEFVGKPFARIKNVMDPGCFAQASTKLASILE
                     GKTVDPSDYIIFAKDGALRYIETNATPVVKNGRLFCIVCVTRDITRRKRMEESLLSER
                     NFSQTIVESMPGLFFVLDEQGKFLHWNDHLKIMTEYSDTEIRERLFTDIRPESQKGAV
                     MEHIREIFRLGEFSWEAEMIFKDGTRKIFRLHAKRMYYEGQACIVGTGMDITDQKRAE
                     NDLKRFAENLQDANIALRVLMQQRDEDQKDIEEKLQANVNDLVIPYLKKLKQADLEER
                     HKNHLQMLEHNLREILSPFMKNLHAAHANLTSREIQIMDLIKQGKSNKEIAAMLNASL
                     KTVETHRNHIRKKLNLVNAGVNLRSCLLSVS"
     gene            complement(7720..8808)
                     /locus_tag="PLA58_04195"
     CDS             complement(7720..8808)
                     /locus_tag="PLA58_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR62029.1"
                     /translation="MISVRHLSKAFGRLVVLKDVNVEIDKGEVISVIGPSGCGKSTFL
                     RCLNLLEMPSGGEIVIDGENLLDRKTDVFQVRRKMGMVFQSFNLFSHLMVIENIMLGP
                     VDLLKRGRREAFAEGMKLLARVGLAEKAYAYPDELSGGQKQRVAIARALAMKPEIVLF
                     DEPTSALDPTMVSEVLAVIRKLAADGMTMMIVTHEMRFARDVSTRVLYMDEGVIYEEG
                     TPRQIYDQPVREKTRNFVHKIRTYSYEIRSRTFDLYALNAGVETFCRKQFLPEKQIFH
                     IQLILEELIINQLLARQEDPVTLDVTVGYSEESGAVEIVLHYAGGPYNPFDEAEEEDN
                     LSMVLLRKLAQRREYVAGDLANQLKIIL"
     gene            complement(8805..10976)
                     /locus_tag="PLA58_04200"
     CDS             complement(8805..10976)
                     /locus_tag="PLA58_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOR62030.1"
                     /translation="MRTLPLPAAQMNTPEYTIGVAQGTTAMSIVEARFPKSRIEYHMS
                     LHDGYLAVQYGKIDAFAFDRHSLQYVAAHNPELALLPEKIGDERIVVGTALGKEALIG
                     RVNDFIRQYRSDGTYADMYRRWIMGQNPKMPDLPKPARPAITLVIGTDGRNEPMNFYE
                     NGRLAGFDIEFAERLALFLNAKPVYHTIDFPAVVSAAATGKIDLLVSSLNATPERRRM
                     MLFSDPYIDSEISFLARADRLPFQTADITRFSQLAGRKVGIITGSTYDRVLKKANPGA
                     VPEYFQTFADQAEALRSGKIAAFLVDEPIARDIMNRTEDLTILPDRLTSDGYAFAFSR
                     RRPDLRRQFNEALEEMRAGGALRKIDAKWFGKNEAAKVLPDLKTDGSNGVIRLATNSG
                     LPPFAYIRDGQIVGYDIEIAMTIAAKFGWTVEISDMEVAAILPSLISGKSDMAACGIT
                     ITEERSRSVLFSAPTYKGGIAVMVKAEGKASKSAAKGGPWARFVESFQRTFLVEKRYE
                     MVLQGLRVTIIITLLSAFFGTLLGFGICMMRRAQTRWANLPARIFIRAVQGTPIIVLL
                     MILYYIVFGSTNLDAIVVAVIGFSINFAAYVSEMMRTGMDAVDRGQQEAAYALGFNRV
                     QVFQKITFPQAARHALPVFKGEFVSMLKMTSVVGYIAIQDLTKMSDIIRSRTYEAFFP
                     LIATALIYFVMAYLMAGLLSLVEIRVDPKRRKRAVKGGVRP"
     gene            11193..12158
                     /locus_tag="PLA58_04205"
     CDS             11193..12158
                     /locus_tag="PLA58_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR62031.1"
                     /translation="MRVYFWGTRGSLPASITSGAIEAKIVKAIRASRARALETDEAVE
                     AFVHNELPFPVRASYGGNTACVEMRGADEYVLCDAGTGLRDFGGAYMKAKASGGQNLP
                     RVFHLLMSHPHWDHLQGFPFFTPAYLPGHRIHIYGGHADLESIFVRQQSEPCFPVPLR
                     AMRADIRFHTLEPGVEYNIAGMKVACIRQNHPGDSYGYRFQKDGRIVVYSTDSEHKAG
                     GGEEHDRFLAFFSNADLLIFDAQYSFLDAVDAKENWGHSSNLLGVEMAAAAGVGRLCL
                     FHNEHTLDDDALDRFLEDTRAYLKILDGSSRLKIDLAYDGLDIKL"
     gene            12228..13088
                     /locus_tag="PLA58_04210"
     CDS             12228..13088
                     /locus_tag="PLA58_04210"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOR62032.1"
                     /translation="MADQLDPKKIREVFRNVRNHREMERIIRRCSTNPEDIRQAALSG
                     VDLTSCRTVLELGCAFGSFTVALKGRLHPRAHITGLDIIPEYEPFFLEACEQAGYPGR
                     FSSDGVHQIAGFPTAGVDLILCSFALYFFVDILPDIARILTPDGRLIAITHASGNMRE
                     LIGAIRNILKTHGVLEPDDVLPIEQILKVFTAENGAALLAPHFVGIRKIDFPNTLVFP
                     PQDMGAFVDYYRFKRPLFLAGTPPAADIIIPELTKLLAGMAARSQTISMCKDDAIFIC
                     SQPHPRKESS"
     gene            13085..14152
                     /locus_tag="PLA58_04215"
     CDS             13085..14152
                     /locus_tag="PLA58_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOR62033.1"
                     /translation="MKKQRKFCVYCGGLIVKKKEDLVWRDFCSVCRCFFYDNPLPVVS
                     AIVDVSRQILLVKRDRAPFKGRWCLPTGFAEAGENIEQAVLRELCEETGIRGRISRLL
                     DVDSYKSRFYGDLIFLTFVVEKTAGKVAAGDDSAQAKFFTIDRIPPLAFRSNRRAVAA
                     YIKSKKDYWAVLDAIKPHGEKTFHAAKQANSLTRRLIGNLSKNREIMIRQWIDDVSVN
                     PSTQEYHRYEPAILYAICDRIISQITRWLGDAYAIEHIKNFYIKLGRERKKSGTLISS
                     ALSALSLIRKHIWDVALARGALQKNLGLYATFELQRRMTVFFDLAAFYIARGYESSKT
                     RPSPAQTPAADSLKTRRRKTI"
     gene            14260..14634
                     /locus_tag="PLA58_04220"
     CDS             14260..14634
                     /locus_tag="PLA58_04220"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOR62034.1"
                     /translation="MKLSEVRDILDAEIIVGEDCLDLEVHTAFGADLMSDVLAFAKAG
                     SLLLTGLTNTQVIRTANVLDIAAIILVRGKKPSSETIALASELKIPILTTKYILFETA
                     GRLYMKGIVGCLSKVENTSGRT"
     gene            14621..15058
                     /locus_tag="PLA58_04225"
     CDS             14621..15058
                     /locus_tag="PLA58_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR62035.1"
                     /translation="MGEPKPNLYSESFHIEGGNFNSAGIVSTSIKSILKKLGIPGDIV
                     RKAAIVSYEAEINVVSYAKKAVVNLTVGPGVVRIEVIDEGQGIPNIDLAMQEGYSTAD
                     QRIREMGFGAGMGLCNIKNYSDDFHIESEVGKGTHLKMTIQTP"
     gene            15081..15422
                     /locus_tag="PLA58_04230"
     CDS             15081..15422
                     /locus_tag="PLA58_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62036.1"
                     /translation="MNVEAIVAAVGLEVKCGRESLGREVTGGYTGDLLSDVMGHSKEG
                     YLWITRQVHQNIVAVASLKEHAGIILINGCEPAAETLEKAEAEKIPVMVSVLPAFELS
                     GRIYNLLTSPG"
     gene            15454..16200
                     /locus_tag="PLA58_04235"
     CDS             15454..16200
                     /locus_tag="PLA58_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HOR62037.1"
                     /translation="MLKVFNCDLHIHTCLSPCAELDMHPKALVERALEQRLDIIAVSD
                     HNASANVPYVMAAAAGKSICVLPAMEITTSEEVHLLALFGRLTDLQQLQAIIDRHLFG
                     ENDEDRFGVQAIVNERGEVEGLNHQLLIGATDLPIDPLVDTIHQLGGLAVPAHIDRES
                     FSVLSQLGFIDESSRFDALEISKRLGIRQGRERYPELARYAFLTSSDAHFIPDIGTAT
                     TPFLMEKPTFDEITMAVSGQNGRQILEQPC"
     gene            16194..16742
                     /locus_tag="PLA58_04240"
     CDS             16194..16742
                     /locus_tag="PLA58_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR62038.1"
                     /translation="MLTLADHILDIAENSVRAGAKLISIFIEEDSANDLLTLEISDDG
                     HGMAKDVLQKVLDPFYTTKTVRRVGLGLPLLKDAAQRAGGTFQLKSEENCGTTVKATF
                     GLRHLDRQPLGAIINTIIILIISNSDIDFFYKHRHNDRQFEMDTRKIRKEIEDIPINH
                     PEVIKYIRRAMEEGLQEIEPEA"
     gene            16772..17266
                     /locus_tag="PLA58_04245"
     CDS             16772..17266
                     /locus_tag="PLA58_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOR62039.1"
                     /translation="MKAEDTELLKEFTPEQVAKLDSIIDKHKGKAGGLIPVLEEAQVC
                     LEYLPMSVQKRIAAGLNLPLSRVYGVVTFYSFFTMTPRGKHTVRVCLGTACYVRGGKA
                     LAEALQKQFGIKEGETTADRLFTLESVRCLGACGLGPVVVVDEDVHGRVKPAKIKEVL
                     SQYN"
     gene            17299..17682
                     /locus_tag="PLA58_04250"
     CDS             17299..17682
                     /locus_tag="PLA58_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOR62040.1"
                     /translation="MAKITIDDLKKIKEKVHKEMSLRDGDRRVKVTVHMGTCGIASGA
                     KEVMDTLLEEIEAAKAQDVIVTTSGCMGLCSREPLVTVEVLNAEPIKYEYVNPNKMRQ
                     IFKRHILAGEIQTPFVLAKGSEVLK"
     gene            17749..20805
                     /gene="nuoF"
                     /locus_tag="PLA58_04255"
     CDS             17749..20805
                     /gene="nuoF"
                     /locus_tag="PLA58_04255"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOR62041.1"
                     /translation="MKVFRSHILICGGTGCQASGSLGVKNALLEELVKRKLAEEIKVV
                     ETGCNGFCALGPIMVVYPEGVIYISLKPADIPELVEEHLIKGRIVDKLLYREPGTDKT
                     IPTMQDIPFFAHQELRVLRNRGLIDPEKIEEYIARDGYAGMAKALTEMTPEQIVQEML
                     DSGLRGRGGAGFPTGLKWKFAAGSKGDVKYVLCNADEGDPGAFMDRSVLEADPHAVLE
                     GMVIAAKAIGSHSGYIYCRAEYPLAIHRLNVAIAQAKEAGLLGKDILGTGFDFDLEIY
                     QGAGAFVCGEETALMTSIEGKRGMPRPRPPFPAVAGLWQKPSILNNVETLANVGQIIL
                     RGAKWHASIGTEKSKGTKVFALTGDVNNVGLVEVPMGTKLGTIVYDIGGGIPKGKKFK
                     AAQLGGPSGGCIPVQHLNASVDYEKVAELGAIMGSGGLIVMNEDKCAVDMARFFMDFC
                     QDESCGKCTPCREGTKRMLEILTNITQGKGKPGDIELLEEMAGVIKNAALCGLGQTAP
                     NPVLSTIRYFRKEYEEHIFQHRCQATVCSALFKSPCQHTCPVEMDIPSYIALVREGRF
                     EDAYKILLQTNPFPSVCGRVCDHKCQSKCRRGNMDEPLAIKFLKRFITDNAPRPKTDP
                     APVTRKEKIAVVGAGPAGLTAARDLALRGYKVTVFEELPYAGGMLRWGIPAYRLPRNI
                     LQGEIDDITALGVEIRLNIRVGKDIAFSQLEKDFDYFYLATGAHKSQKMRVEGEDLAN
                     VFGGVEFLRDFNNNEDKWLRGEKTLGKKVAVIGGGNSAIDAARVALRLGSEVTILYRR
                     LRQDMPAAEEEIKAAEEEGIKIEYLIAPLKIDGTKGKVSSITCQRMKLGDFDNSGRKK
                     PAAIEGSEFTLAVDAIVAAIGQVPDMSFIDKKVGVEINKWDCYTVGKGFKSRTTNPRY
                     FAGGDAETGPDTVIGAIAAGHQAADDIDAAIRQANHEPAYEKPALEKIDVPLVIDDET
                     TETPQMPMPEMHHATRKMSFAEVELGFSKEDAMKEACRCLRCDASV"
     gene            complement(20903..>22035)
                     /locus_tag="PLA58_04260"
     CDS             complement(20903..>22035)
                     /locus_tag="PLA58_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR62042.1"
                     /translation="RRRKSMASFTKPESYRENVENISKLTFAVGVYRPPSEGGSASLL
                     LRITENCPWNKCTFCEMYKGHPFGYRPVADVKADIDTVKAISDEIREISIKTGNDGRI
                     NREVLQAILSVDPYLRENYCFSSVFNWLYYGGKTAFLQDANSMIMRPGEFVEVLQHLR
                     KTLVSLTRVTSYTRSKTLAQRKLEELVAIRKAGLDRIHVGLETGDDEILKIVRKGVAS
                     EEQIEGGKKAMAAGFQLSEYWMPDLGGRERWRRHAENTARVLSAINPHYIRSRPLVPR
                     QGTELFEEYEQGRLHISSPHERLEELQVMIENLNVTSRVCFDHNMNAWTNRQGGLLFS
                     QSYEGYAFPEEKPHVLELIREGLSVEESRHINIKELIALGSL"
BASE COUNT         4980 a         6394 c         5973 g         4688 t
ORIGIN      
        1 gatttcatct atcctcttca acgtcgtcag cagttcttcg agcgaatgga gataaagcga
       61 attggcgccg tcgcatagcg ctttttcagg atcgggatgc acttccatga aaatgccgtc
      121 cacgcccgcc gcgacggccg cccgcgccag gtagggcacc atatcccgct ggccgccgga
      181 cgaggttccc atgccgcccg gcaactgcac cgaatgcgtg gcgtcaaaaa taaccggata
      241 tcccatcgcc cgcatgatcg gaagcgcgcg cacatcgaaa accaggttgt tgtatccgaa
      301 ggacgcgccg cgttcggtga tcagaatatt ttcattgccg gccgactgaa tcttcccgat
      361 gatgttggcc acgtcccagg gcgcaagaaa ctgtcctttc ttcacgttca cgatcttcgc
      421 ctttttcgcg acctccacga caaaatccgt ctgccggcac agaaacgcgg gcacctgcat
      481 cacatccagg acgccggccg cttcgtcaat ttcttcaaac cgatgcacat cggaaaggac
      541 cggcgcgccg attttttccc gaatgttttt taaaattttc aggcccgcgc ggacgcccgg
      601 tccccggtag gcggcaatcg atgtccggtt ggccttgtcg taagacgctt taaagatgaa
      661 aggaatctcc agtttgtccg tcagcttttt cagatacgcg gcaatcttca ttgccgaggt
      721 ttcattttcc agaacacagg gaccggccat caggataaat ccggccccgc cgccgatcgt
      781 ggaagaattc agtttcacct gcttcatgac tcgctcccct tgtcgtcttt tttatgcaaa
      841 agttgaatac gcagcctcgg aatccgggat gccacccgcg tttccggatt gatctcgtag
      901 gcgcttctat aagctttcag cgcttcaggg taaagccctt ttttctcata agcgatgccc
      961 aggttggcgt agatctcgtc atcctcgcgg tcgtatttca gagcctgcaa ataattctca
     1021 atggctttat ccgggtttcc ggaagccagc caggcatacc ccagagcggc ccaggaagat
     1081 gcctttttgg gctccagctt tgatattttc tcataatttt tgatggcttg ggcgtatttc
     1141 ttctctctca gataatggtc ggcaagaatg ctcaatattt ttttgttttg aggcgatatt
     1201 ttttcgtagg cgcggacggc gtccttttcc ctccccagct tcgtgtaggt aaacgccaga
     1261 ttgtaataca gggtctggtc tttcgccccc agccggagag ccttttcgta atcttcagcc
     1321 gatgcggcgt attgcttcag ttcgccgcgg gcaaatccga gcctggcaaa cacggacgcc
     1381 tgctgcggat gaatccgcga cagtttttcg taataacgga cggcctggtc atactgtttg
     1441 ctttgaaagg tcaggtccgc cagacgcgcg agcgcgtcag catcatccgg attgctcttc
     1501 agaacccggg tgtattcccg aatggcttcg gatgttcttt gtcttttttc gtaggcggcg
     1561 gcaagattga agcggatcac cggttcgtcg ggcgacaggg ccgcggcctt ctgcagatgc
     1621 tccatttcct ccttcgcttt accctgcgcg gcgtacgccg tggccagatt ggcatgcgcc
     1681 gccgcgtttt tcggatggat ggccagaacg cgcgtatagc tctcgatcgc ctgtgcgtat
     1741 tgcccgctcc tcaaatgcat atcgcccatc gccagccagg ccggggcggc atcgggcgaa
     1801 tggctgatga tgaagcggta ctgttcaacc gccgcgtcgt tccggtggga cttctcgaag
     1861 gcttgggcca gcaaaagccg caccggataa ttttccggat ccattttgac ggcccgggcg
     1921 tagctttcca ccgcctggtc ccacagattt ttgcgcccgt aggcggcgcc gagcatcgcc
     1981 tgggcttcgg catcctgtgc ctggatcccc gtcaacctgg tcaaaagatc gatggcgcgg
     2041 tcgtcctggc cgagccgcac gctgacgcgg gccaattcct tcatcgccgg gacatcgccg
     2101 gggctgattc gcaaaatctc ttcatacatt ttcgcggctt ccccgatgcg gttttgctga
     2161 agatagatgc ctgccagaat tttgcgcacc cccgcatctt ctcggttccg ggcagcaacc
     2221 ttcttcaggt actcctcctg ttcgcccgaa ttgccgcggt tctttgcgaa cctcagccaa
     2281 tcctgggggg taaggaccac ccggaccggc acaacggcga ttttgtcccg cgcggcatag
     2341 acaacgatct gataaacgcc cgcggtctca gtcccttcag gaatttctcc gccggtgaga
     2401 aggttgtcca ccagatcaat cccgcgaaag aggacgccga aggcattgcg ttggctgcca
     2461 gccccctcca gaaacaccgt cacggatttt ccgcttagat catcgctggc cacggatttg
     2521 accacgaatt catcgcgata ggtaacatcg agcgtttcac ctgccttgac gcggaaatcg
     2581 ctcgcgtttt tctccatcac cagacaataa aaatgcggcc ggtctcccaa aacgtagtac
     2641 gcaaacgcgt tgatccccct ttgaacataa aagccggccg tccggaaggc ctgggggtaa
     2701 aacgccagca gcaaaaacaa aaccagcgcc gctgtccccg ccgctgcaat ggccggaatt
     2761 tttttgccga atccgatttt atacccggtt gattttcgca aacgttcagc ctcttggatg
     2821 atatttccgg tgaatctctt tcaggcgttc ccgggtgacg tgcgtataaa tctgcgtcgt
     2881 cgaaatgtcc gaatggccca gcatcacctg caccgcgcgc aaatccgcgc ctccctccag
     2941 caggtgggaa gcaaaggaat ggcgaaatgt atgcggatgc acattttttt gcaagcccgc
     3001 tttttgcgca taccccacga tgattttcca gagcccctgg cgggtaaagg cccggccgaa
     3061 gcggttcaga aacaacacct ccgtcgtttt ggaacggacg aacttcggtc tggcttcatc
     3121 cacgtagcgc cgcacaaagt cataggccgt ccggcctacg ggcaccacac gctccttgcc
     3181 gcccttcccc atgaccgcca gaaaaccaac ctgccagttg atgctgttca tggaaaggct
     3241 gatcagctcc gtgacgcgaa gtcccgtcgc gtagagcagc tccagcatcg cccggtcccg
     3301 cagacccgac ggggtcgagt gatccggccc cgacaggatt cggttcatct cttcccggct
     3361 taccgtatcg ggcagacgca tccagacttt ggccagttcg atatgcgtca gcggtgattc
     3421 ttccagaact ctttcctgca gcaggtagtt ataaaaaccg cgaagggccg caagggcccg
     3481 gttcatcgaa ttggccgaaa gcccttccga tttgatctgc acgagaaaat ctacaacggt
     3541 ttgcggggcg accgaccgcg cgtcagaaat ctccttttgc tcgaggaaag tcaaatagcg
     3601 tttcaaatcg cgtccatacg cttccagcgt attgccggac gctcctttct cgatcagcaa
     3661 ataacgatga taccggttta ataaatcccg catggcattt ttctacgcaa aatggatgct
     3721 gatgtaaaga ttttttgttg acatcccgcg tcatcttgta gaaagaatca acatcgatgt
     3781 tcagggaggc gtgccaatga aaaaaatcgt catcgccagc gatcacggag cggttgatct
     3841 gaaagagagg attgttgcct ttttgcaaaa gaaaaattat gaggtcaccg atctgggaac
     3901 gcacggctgc gaatccgtcg attatccgga catagccgcg gccgcctgcc gtgaattcct
     3961 caaaggcggc tacgaattcg gcatcctgct ttgcggcacg ggtatcggcg tttcgatcgc
     4021 cgccaacaaa atcgccggca tccgctgcgc tctcgtgtgc gatttgttca gcgccgagat
     4081 ggccagggca cacaataacg ccaatttcat cgccttcggc ggaagaatac gctacgccgc
     4141 aacggtcgag gaaatgatcg ggaaatttct ggacacaacg ttcgaaggcg gccgtcatga
     4201 acgaagaatc gccaagctga ccgccctgga aaacagccgc tgatccgggg cgccgcgcgc
     4261 tccggcgcgg gcgttttccc gtttattttg cggtgatcct gaacgtcctc tccagcggag
     4321 cttcactttc cggcccgaac cacttgtcaa agattcgctg ggcttcgccg ctgcgctcca
     4381 tctccagaag cgtctgattt acataacgca aaaaggaggt atggcctttg cgcaccccca
     4441 gaccataggg ctctctggaa atgctgactt tcggaatttc aaatacgcct ttttcggcct
     4501 tcgtcagaat gccggccagg atggcctcat ccgtcgaaac ggcgaagatt tccctgttct
     4561 gcagcgccag aaaggccgga acataaccgg tgaaaagaac gatcgacgcc gaggggatgg
     4621 atttgcggag attcgtaacc gaggtggtct gatccacggt cccgatcttc ttgttcttca
     4681 aatcgctcag ccgctgcacg gttcccttgt gtaccagaaa cttctgggcc gtgacaaaat
     4741 atgtatggct gaagtcgatg agcttttctc tttccttcgt tttggtcatg gtggccgcaa
     4801 cgatatcaac cctgccctcc gtcagggcgt caatccgttc attttcctct ccgctgaaag
     4861 gcgtaatctc cagacggacg ccaagcctgc gggcaatcgc ccggacaaaa tccacgtcat
     4921 acccgctgat ttcccccgtc ttgatgtcgg ccagcgcgaa gctcggggtc agatccttga
     4981 cgccggcccg taaaacgccg cttttcctga tgttttgcag agagtcttcc gcctgggcca
     5041 cgcagcagat ccccgccgca agcaatagta tcgtgaacag acatacgatc tttttcatag
     5101 ttatcctcca tcctccttaa aaatattcat tctttcgtca tgcgggcgaa cctgcgcagg
     5161 tgatccgtgt aattctccct gtgcagggtg accacccggg ccagatacgc catatttttt
     5221 tcaatgcttt tgatgtagag atcttcatca atgccccagc gctttcggaa atgaaatcgg
     5281 gaaaaccgaa gcgtgttgtc caattcctcc gcgaatcgct tcagcgtcat ctgataaaat
     5341 ccctgcgtct tgtccaaaag atccagcggg ccggcaaagg tcgtgatgcc ggcctccgca
     5401 aattcatcga agagatccgg cagcttttcc agataataac gatcggccat ctgacccaga
     5461 agatccgccg tccccagaat tttccccagc agttcaaccg tcgcgccgcc aaaccggatg
     5521 gacttcacct gagtgttgag ccccgtgcac agcaaaatgt cccgaaatga atccagatcg
     5581 ttttggaacg ccggatcatt gcggtaatag tcttgaacaa aggcgatgct gcggctgata
     5641 tgaacctgcg tgtatttcgc cccggtcccc tcccggtcgg aggtttgctg aatatatccg
     5701 gtatcgtgca tcagaccgct gatgaatccc atgttcaggt ctttttcgga aagcgattgt
     5761 ccggcttcca cggccccgtg catcaggcgg gcgacggcaa gcgtgaccga aagggtatgg
     5821 ctgagatcgt ggtacctcac gttgcaggag cgataggacg gatagtttcc gcgaaaaagg
     5881 gcgatcacgt ccgaataggc ccggtcaaaa ggcgcaacgt caaaccgcgg aaacatcaga
     5941 ccggcgatcg tatggatttc gtcccgaacg gctttggggt cccgggtgtc cacaagattg
     6001 gccagctgca cgcgacaatc gtccatcagc gctccccgtc agcggcgcct cgcgcagaga
     6061 agtttatggt tctccggtct tggcccgtga tgccctgggc cacggccggc cgttttcccg
     6121 gcatcgcgcc gctgattcca tgatgatggc tggcaatgtg acacatttct ccaaattcat
     6181 caacggataa agatgctgaa gagcaaaaat caggcccggc acccgtgcag gcgcgctccg
     6241 gcgctttccc cgcaccgaat tgcgttttgt taagattttg ctcttgcctc ggcctgaaaa
     6301 aagctttaga tatgcgatca tcatccgtga acggatttca cgcgaggcgg ccatgaaaaa
     6361 gaaaatcccg gacaaaattc tcctctccaa gccggaaaag aaaatggcgg atctggaaaa
     6421 agccgacgaa gagctccgga acctggagga aacataccgg ctgagctttg aacaggtgtc
     6481 ggatgtcatt ttcatactcg acacgaacct ccggatactg agcatgtccc cctctgtgga
     6541 aacaaacctg ggctatcatc cggtcgaatt tgtcggcaaa ccgtttgcca ggatcaaaaa
     6601 tgtgatggat cccggttgct tcgcacaagc cagtaccaag ctggcctcca tcctggaggg
     6661 aaaaaccgtt gatccatcgg actatatcat cttcgccaaa gacggagccc tgcgatacat
     6721 tgaaaccaac gcgacccccg ttgtgaaaaa cgggaggctc ttctgcatcg tctgcgtcac
     6781 gcgcgacatc accaggcgca agcgaatgga agaaagcctc ctttccgagc ggaacttcag
     6841 ccaaaccatc gtggaaagca tgccgggcct gtttttcgtg ctggatgagc aggggaaatt
     6901 tcttcattgg aatgaccatc tgaaaatcat gaccgaatat tcggacacag agatccggga
     6961 gaggctgttc acggacattc gccctgaaag tcagaagggc gccgtcatgg agcacatccg
     7021 ggagattttt cgtcttgggg agttctcctg ggaggcggaa atgattttca aagacgggac
     7081 ccgcaaaatt ttccggctcc atgcaaagcg catgtactac gaaggccagg catgcatcgt
     7141 aggcaccggc atggacatca ccgatcagaa gcgggccgaa aacgatctga agcggtttgc
     7201 cgaaaacctg caggacgcca acatcgccct gcgcgtttta atgcagcagc gcgacgaaga
     7261 ccagaaggac attgaagaga aattgcaggc caatgtgaat gatttggtta ttccttattt
     7321 gaaaaagctg aagcaggccg atctggagga acgccataaa aaccacctcc agatgctgga
     7381 gcacaatctg cgggagatcc tttccccgtt catgaaaaac cttcatgccg cgcacgcgaa
     7441 cctgacctcc agggagatcc agatcatgga cctgatcaaa caggggaaaa gcaacaaaga
     7501 aattgccgcc atgctgaacg cttcgttaaa aacggtggaa acccatcgga accatatccg
     7561 gaaaaagctc aacctggtca atgccggggt caatctccgg tcctgccttc tttccgtgtc
     7621 gtaaccgccg gggcgggagc gaagacaacc tcgcggcgcg gccgtccgga taaccgcgcc
     7681 gctgccgctt tgccgttttg caaaatcagg cctgcctgac tacaggataa ttttcagttg
     7741 attggcgagg tctccggcaa catactctct tctttgggcc agtttccgga gaagaaccat
     7801 ggacaggttg tcttcctctt cggcctcgtc aaagggattg tatgggccgc ctgcgtaatg
     7861 aaggacgatc tccacggcgc cgctttcttc ggaatagccg accgtgacat ccagggtgac
     7921 cggatcctcc tgacgggcaa gcagctggtt gatgatcagc tcttccagga tcagctggat
     7981 atggaagatc tgcttttccg gaagaaactg tttcctgcag aaggtttcaa ctcccgcgtt
     8041 cagggcgtac agatcaaacg tccgggagcg gatttcatac gaataggttc tgatcttgtg
     8101 gacaaaattc ctcgtcttct cccgcaccgg ctggtcgtat atctgccgcg gcgttccttc
     8161 ctcgtagatg actccctcgt ccatatacaa cacccgcgtg gaaacgtccc gggcaaatct
     8221 catttcatgc gtaacgatca tcatggtcat gccgtcggcc gccagcttgc ggatgacggc
     8281 cagcacctcg ctcaccatgg tcgggtccag cgcggacgtc ggctcgtcaa acaggacgat
     8341 ttccggcttc atggccaggg ccctggcaat ggctacccgc tgcttctgtc cgcccgacag
     8401 ttcatcgggg taggcatatg ctttttccgc cagccccacc cgggcgagca gcttcatgcc
     8461 ttccgcaaag gcctcccgcc gtcccctctt caaaagatcg accggcccga gcataatatt
     8521 ttcgatcacc atcagatgcg aaaaaagatt gaaggactga aacaccatgc ccattttccg
     8581 ccggacctga aagacatcgg ttttccggtc gagaagattt tccccgtcga tgacgatttc
     8641 acccccggac ggcatctcca gaagattgag gcagcgcaga aacgtgcttt ttccgcagcc
     8701 ggacgggccg atgacggaaa tcacctcgcc tttgtcgatt tccacattca catctttgag
     8761 aacaaccagc cggccgaagg ctttggacaa atgtctgacg ctgatcatgg gcgtacaccg
     8821 cctttcaccg ctcttttgcg ccgtttcgga tcgacccgta tttccaccag ggaaagaagg
     8881 ccggccatca gataagccat gacgaaatag atcagggccg tggcgatgag cggaaagaag
     8941 gcctcatacg tgcggctgcg aataatatcg ctcatcttgg tcagatcctg aatggcgatg
     9001 tagcccacca ccgacgtcat cttcagcatg gagacaaact ctcccttgaa caccggcagg
     9061 gcatggcgcg ccgcctgtgg aaaggtgatt ttctgaaaca cctgaacccg gttaaagccc
     9121 agcgcgtagg cggcctcctg ctgccccctg tccacggcgt ccatgccggt gcgcatcatt
     9181 tccgaaacat aggcggcaaa attgatcgaa aatccaatca ccgccacgac aatcgcgtcg
     9241 aggttcgtgc tgccgaaaac aatgtagtat aaaatcatca gcagcacgat aatgggcgtt
     9301 ccctgaaccg cacggataaa aatccgggcc ggaaggttcg cccaccgcgt ctgagcccgc
     9361 cgcatcatgc agatcccgaa gcccagcaat gtgccgaaaa acgcggacag gagcgtaatg
     9421 atgatggtca cccgaagccc ctgcaaaacc atttcatacc gtttttcgac gaggaaagtt
     9481 ctctggaaac tttcgacaaa ccgggcccac gggccgccct tcgccgcgct tttggaagct
     9541 ttcccctcgg ccttgaccat gacggcaatg ccgcctttgt acgtgggagc gctgaaaaga
     9601 accgacctgg accgttcttc ggtaatcgtg atgccgcacg cggccatatc gctttttccg
     9661 gagatcaggg atggaagaat tgcggcgact tccatatcgg aaatctcgac ggtccagccg
     9721 aatttcgcgg caatggtcat cgcgatctca atgtcatacc ccacgatctg cccgtccctg
     9781 atatacgcaa aaggcggcag gccgctgttg gtggccaggc ggatgacgcc gtttgagcca
     9841 tcggtcttga ggtccggaag gaccttggcc gcctcgtttt tgccgaacca tttggcgtcg
     9901 atttttcgaa gcgcgccgcc cgcccgcatc tcttcgagcg cttcattgaa ctgccggcgc
     9961 agatccggcc tgcgcctcga aaacgcgaaa gcgtatccgt ccgaggtcag ccggtccggc
    10021 aggatcgtca gatcttcggt gcgattcatg atgtcccgcg cgatgggttc atccacgaga
    10081 aaggccgcaa tcttcccgga ccggagcgct tcggcctggt ccgcgaacgt ctggaaatat
    10141 tccggaacgg ctccggggtt cgcttttttc agcacacggt cgtaggtgga accggtaata
    10201 attccgactt tgcgtccggc cagttgtgaa aaccgcgtga tgtccgctgt ctggaaagga
    10261 agccggtctg cgcgcgccag aaaggaaatt tccgaatcga tataaggatc ggaaaaaagc
    10321 atcattcggc gacgttccgg cgttgcattc aggctggaaa ccaacagatc gattttgccc
    10381 gtggcggcgg cgctgaccac cgccggaaag tcaatggtgt gataaaccgg ttttgcgttc
    10441 agaaacagcg cgagccgctc ggcaaactct atatcgaacc ccgccagacg gccgttttcg
    10501 taaaaattca tcggctcgtt gcggccgtct gtcccaatga ccagggttat ggccggacgc
    10561 gccggtttcg gcaggtccgg cattttcgga ttttgcccca taatccaccg gcggtacata
    10621 tccgcatagg ttccgtcgga ccggtattgc cggatgaaat cgttgaccct tccgatcaac
    10681 gcttcctttc cgagcgcggt gccgacaaca atgcgctcat cgccgatttt ttcgggaagc
    10741 agcgccagtt cggggttatg ggcggcaacg tattgaagcg aatgccggtc gaaggcaaac
    10801 gcgtctattt tcccgtactg aacggccaga tacccgtcgt gcagggacat atggtattcg
    10861 atgcggcttt tcggaaaccg ggcttcgacg atcgacatgg ccgtcgtacc ctgcgccacg
    10921 ccgatcgtat attcgggcgt attcatctgc gcggcaggaa gcggcagcgt acgcaccttc
    10981 ggctcattgg agcacccggc cagccaaccc gtccccaaaa aaacgaccag cgcaaaaaag
    11041 gcgcctctct cccaaaatgt ttttttacga cagaccccca cgcttcacag cctcgccagg
    11101 gataattttc gttgcgatgc gctcgacaat atgacaaaat gcctgtgtct ttgcaatgga
    11161 ataaatgcgg atgaagaagc gggggaagga aaatgcgggt ttatttctgg ggcacaagag
    11221 gatctttgcc cgcctcgatc acctcggggg cgatcgaggc aaaaattgta aaagccatcc
    11281 gtgcctcccg cgcccgtgcg ctggagacgg acgaggccgt tgaagccttc gtccacaacg
    11341 aactcccctt tccggtccgg gcaagctacg gcgggaatac ggcatgtgtc gagatgcgcg
    11401 gcgcggacga atatgtcctt tgcgacgcgg ggacggggct ccgggatttc ggcggcgcct
    11461 acatgaaggc aaaggcgagc ggagggcaaa acctgccgag ggtctttcac ctgctgatgt
    11521 cgcatccgca ctgggaccat cttcagggct ttcccttttt tacgcccgcc tatctgccgg
    11581 gccaccgtat ccacatttac ggcggccacg cggatctcga atccattttt gtccgccagc
    11641 agagcgagcc ctgctttccc gttccgcttc gcgccatgag ggccgacatc cgtttccaca
    11701 ccctcgagcc cggagtcgaa tacaacattg ccggaatgaa ggttgcctgc atccggcaga
    11761 accatccggg agactcctac gggtaccgct ttcagaaaga cggccggatc gtcgtctatt
    11821 ccaccgactc ggagcacaag gcaggcggcg gggaagaaca cgaccggttt ctggcttttt
    11881 tttcgaacgc cgatctgttg attttcgacg cgcaatacag tttcctcgac gcggtggacg
    11941 ccaaagaaaa ctggggtcat tccagcaatc ttctgggcgt cgaaatggcg gcggccgccg
    12001 gcgtcgggcg tctgtgcctt ttccacaacg aacatacctt ggacgacgac gcgctggacc
    12061 ggtttctgga agacacccgc gcctatctca aaatcctgga cggttcgtcc cgtctgaaga
    12121 ttgatctggc ctacgacggc ctggacatca aactttagcc ggacgggcgc gcattccccg
    12181 cgcaaacgcc cgtcccgaac accggctgga gccctttgag ggaaagcatg gcggaccaac
    12241 tcgatcccaa aaaaatccgt gaggttttcc gcaatgtccg caatcaccgg gagatggagc
    12301 ggattatccg ccgttgttcc accaacccgg aggatatccg gcaggcggcc ctgtccgggg
    12361 tggatctgac ttcgtgccgg actgtcctgg aactgggctg cgcgttcggc tccttcaccg
    12421 ttgctctcaa ggggcgtctt cacccgcggg cgcacattac cggactggac atcattcccg
    12481 aatacgagcc cttttttctg gaggcctgcg agcaagccgg ctacccgggg cgcttttcca
    12541 gcgacggcgt gcatcagatt gccggcttcc cgacggccgg cgttgatctg attctctgca
    12601 gctttgccct ttactttttt gtggatatcc ttccggacat cgcccgcatt ctgacgccgg
    12661 acggccgcct gatcgcgatt acacacgcct ccggaaacat gcgggagctg atcggggcta
    12721 tccggaacat tttgaagacg cacggagtcc tcgaaccgga cgacgtcctg cccattgagc
    12781 aaatcctgaa ggtatttacg gcggaaaacg gcgcagcgct tctggccccg catttcgtcg
    12841 gcatccggaa aatcgatttt cccaacacgc tggtttttcc cccgcaagac atgggagcgt
    12901 ttgttgatta ttaccgtttc aagcgcccgc tgtttctggc ggggacgccg cctgccgccg
    12961 acatcatcat ccccgaattg accaaactgc ttgccggcat ggcggcccgt tcccagacca
    13021 tctccatgtg caaggacgat gcgattttca tttgttcaca acctcatccc cggaaggaat
    13081 cgtcatgaag aagcaaagga aattctgtgt ttactgcggc ggtctgattg tgaagaaaaa
    13141 ggaagatttg gtctggcggg atttttgttc ggtgtgccgc tgtttttttt acgacaatcc
    13201 gctgccggtg gtctcggcaa ttgtggacgt atccaggcag atcctgctgg tgaagcgcga
    13261 ccgggcgccg tttaaaggcc gctggtgtct cccgaccggc ttcgcggaag ctggtgaaaa
    13321 catcgagcaa gccgttcttc gcgaactttg cgaagaaacc ggcattcggg gccggatcag
    13381 ccggcttctg gacgtcgatt cctataaaag ccggttttac ggcgacctga tctttctgac
    13441 gtttgtcgtg gaaaaaaccg ccggaaaagt cgcggcgggc gacgacagcg cccaggcgaa
    13501 gttcttcacg atcgaccgca tcccgccgct cgctttccgc tcgaaccggc gcgccgtagc
    13561 ggcatacatc aaaagcaaaa aggattactg ggccgttctg gacgccatca aaccgcatgg
    13621 ggaaaaaacc ttccacgccg cgaagcaagc caattccctc accaggcgcc tgatcggcaa
    13681 cctttcgaaa aaccgggaaa tcatgatccg tcagtggatc gatgacgtgt ccgtcaaccc
    13741 ttccacgcaa gaataccacc gctatgaacc ggccatcctt tacgccatct gtgatcgaat
    13801 catctcgcag attacacgct ggctgggaga cgcttacgcc atcgaacaca tcaaaaattt
    13861 ttacatcaag ctggggcggg agcgaaaaaa gtccggaacg ctcatcagca gcgcactgag
    13921 cgccttgagt ctgatccgga agcacatctg ggacgtcgct ctggcccggg gcgcactgca
    13981 gaagaacctc ggcctgtacg ccacatttga gctgcagcgg cgcatgaccg tgtttttcga
    14041 tctggccgct ttttacatcg cgcgagggta tgaatcctcg aaaacgagac cgtcgccggc
    14101 gcaaactccc gccgccgatt ctttaaaaac aaggagacga aaaaccattt aatcttattg
    14161 acgcaaatta tcagccatga tagaaaggta tgccttttta cgaacgcgat gcgcgcggca
    14221 gttcattaaa ccattttgcg gatcgatgag gagcagcctt tgaaactgag tgaagtgaga
    14281 gatattctgg acgcggaaat cattgtcggt gaagactgtc ttgacctgga agtgcacaca
    14341 gccttcggcg ccgacctgat gagcgacgtg ctggctttcg ccaaggcggg cagcctgctc
    14401 ttgaccggcc tgaccaacac ccaggtgatc cggaccgcca acgtactgga catcgccgcc
    14461 attattctgg tgcggggcaa aaaaccgtca tccgaaacga tcgctttggc cagcgaactg
    14521 aaaattccca ttctgaccac caaatacatt ctctttgaaa cagcgggacg tctttacatg
    14581 aaaggcatcg tcggctgcct gtcaaaagtt gaaaacacca gtgggcgaac ctaaaccaaa
    14641 cctctattcg gaaagttttc atatcgaagg cggcaacttc aattccgcag gcattgtatc
    14701 gaccagcatt aaatccattc tgaaaaaact gggcattccc ggagacatcg tccgaaaagc
    14761 cgccatcgtc tcgtacgagg cggaaatcaa tgtcgtgtcc tatgccaaaa aagccgtcgt
    14821 gaacctgacc gtcggacccg gcgtggtgcg catcgaagtc atcgatgaag gccagggcat
    14881 tcccaatatt gatctggcga tgcaggaagg gtattccacc gccgatcaga gaatccggga
    14941 aatgggattc ggcgcgggga tggggctgtg caacatcaaa aattattccg atgatttcca
    15001 tatcgagtcg gaggtcggca agggaacgca tctgaagatg accattcaaa caccataacg
    15061 gaagagcaaa ggattaccct atgaacgttg aagcaattgt tgcggctgtg gggctggagg
    15121 tcaagtgcgg cagagagagc ctgggcagag aggttaccgg cggctacacc ggagacctgt
    15181 tgagcgatgt catgggccac tccaaagagg gctatctctg gatcacccgc caggtgcacc
    15241 agaatattgt cgccgtcgcc agcctcaagg agcacgcggg cattatcctg atcaacggct
    15301 gtgaaccggc cgcagaaacg ctggagaaag ccgaggcgga aaagattccc gtgatggtgt
    15361 ccgttctgcc cgcctttgag ctctcgggca ggatttataa tttactgacg tccccaggct
    15421 gatcgtcgac gacaccctcc ccgggcggaa ccgatgctga aagtttttaa ctgcgatctt
    15481 catatccaca cctgcctgtc gccctgcgcc gagctggata tgcatccgaa agccctggtg
    15541 gagcgggccc tcgagcagcg cctcgacatc attgccgtga gcgaccacaa cgcttccgcg
    15601 aatgtcccct acgtgatggc ggccgccgcg ggaaaaagca tctgcgttct ccccgccatg
    15661 gaaatcacga ccagcgagga agtgcacctg ctggcgctct tcggacgtct gaccgacctt
    15721 caacagcttc aagcgatcat tgaccggcac ctgttcgggg aaaatgatga agaccgtttc
    15781 ggcgtgcagg ccattgtcaa tgagcggggc gaagtggagg gattgaatca tcaactcctc
    15841 atcggcgcga cggatttgcc catcgatccc ctcgtggaca cgattcatca gctgggcggc
    15901 ctcgccgttc ccgcccacat cgaccgcgaa agcttcagcg tattgagcca gctcggattt
    15961 attgacgaaa gctcccgttt tgacgcgctg gaaatctcca aacgcctggg catccggcag
    16021 gggcgcgagc ggtaccccga actcgcccgg tatgcctttt taacgtcttc cgacgcccac
    16081 tttatcccgg acatcggaac agcaaccacg ccgttcctta tggaaaagcc gaccttcgac
    16141 gaaattacga tggccgtctc cggacaaaac ggccgtcaaa tcctggagca gccatgctga
    16201 cgctggccga tcatattctg gacatcgctg aaaattccgt gcgcgcgggc gcgaaattga
    16261 tttccatttt cattgaggaa gattccgcaa acgatttgct gacgcttgaa atttcggatg
    16321 acggccacgg catggccaaa gacgtcctgc aaaaagtcct ggaccccttt tatacgacca
    16381 aaacggtccg gcgcgtggga ctgggtctgc ccctgctgaa ggacgccgcc cagcgggccg
    16441 gcggcacatt tcaattgaaa tctgaagaaa actgcggaac tacggtaaag gccacgtttg
    16501 gcctgcgtca tttggacagg cagcccctgg gtgctattat taacacaatc attattctca
    16561 ttatttctaa ttctgatatt gatttttttt ataaacacag gcataatgac cggcagtttg
    16621 agatggatac gcgtaaaatc cgcaaagaaa tcgaggatat accgattaac catcctgaag
    16681 tcataaaata tatccggcgc gcaatggaag aagggttgca ggagatagaa ccggaagcct
    16741 agcgcgccgc gaagatttga ggaggacgta catgaaagct gaagatacag agttgttgaa
    16801 agagttcact ccggagcagg tggcaaagtt ggacagcatc attgacaagc acaaaggaaa
    16861 ggcgggcggc ctgattcccg ttctcgaaga ggcacaggtt tgtctggaat acctgccgat
    16921 gtctgtgcag aaaagaattg ccgccgggtt gaatctgccg ctgagccgcg tctatggcgt
    16981 ggtcaccttt tattcgttct tcaccatgac cccgcgcggc aagcacacgg ttcgcgtctg
    17041 tctgggcacc gcctgctacg ttcgcggagg caaggcgctt gcggaggcgc tccagaaaca
    17101 gttcgggatt aaagaagggg aaacgacggc agaccgcctg ttcacgctgg aatccgtccg
    17161 ctgtctcggc gcctgcggtc tgggcccggt cgttgtcgta gatgaagatg ttcacgggcg
    17221 cgtcaagccc gccaagatca aagaggtcct gagtcaatac aattaattac cgatcaaaag
    17281 ataacggagg acacgcccat ggcgaaaata accatcgatg atttgaaaaa aattaaggag
    17341 aaagtgcaca aggaaatgtc tttgcgcgac ggcgatcgcc gcgtcaaagt cactgttcat
    17401 atgggaacct gcggcatcgc ttccggcgcg aaggaagtga tggacacgct gctggaagaa
    17461 attgaagccg ccaaagcgca ggacgtgatt gtcaccacct ccggctgcat gggcctgtgc
    17521 agccgcgaac cgctcgtaac cgtcgaagtt ctgaatgccg agccgatcaa atacgaatac
    17581 gtcaatccca acaaaatgag gcagattttc aagcgccata ttctggcggg agaaatccaa
    17641 acgccttttg tgctggccaa aggctctgaa gttttaaaat aaacagttca tcatgctcgc
    17701 ggccgtcatg cgaaccgcgg tctgatgatt aaggaggata ttggattcat gaaagtattt
    17761 cgttcacaca tactgatttg cggcggaacg ggctgccagg cttccggcag cctcggcgtc
    17821 aaaaatgccc ttttggaaga actggtcaaa agaaaactgg ccgaagagat caaagtggtc
    17881 gaaaccggct gcaacggatt ctgcgcgctg ggccccatca tggtggttta cccggaaggc
    17941 gtcatctaca tcagcctgaa gccggccgac atccccgaac tggtcgaaga acacctgatc
    18001 aaaggccgca ttgtcgataa gctgctttac cgcgaaccgg gcacggacaa aaccattcct
    18061 accatgcagg acatcccctt cttcgcccac caggaactgc gggtgctgag aaaccgcgga
    18121 ttgatcgatc ccgaaaaaat cgaagaatac attgcccgcg acggctatgc cggcatggcc
    18181 aaggcgctca ccgaaatgac gccggaacag atcgtccagg aaatgctgga ttcggggctg
    18241 cggggccgcg gcggcgcggg cttccccacc ggtctgaaat ggaagtttgc cgccggctcc
    18301 aaaggcgacg tcaaatacgt tttatgcaac gccgatgaag gcgacccggg cgcgttcatg
    18361 gaccgcagtg ttctggaagc ggatccgcac gccgttctgg aaggcatggt catcgcggcc
    18421 aaagcgatcg gctctcattc cggctacatt tactgccgcg cggaatatcc gctggccatc
    18481 caccgcctga acgtcgccat tgcgcaggcc aaagaagccg gcctgctggg caaggacatc
    18541 ctcggcacgg gctttgactt cgatctcgaa atttaccagg gcgcgggcgc gtttgtctgc
    18601 ggcgaggaaa cagccctcat gacctccatt gaaggaaaac gcggcatgcc ccgaccccgt
    18661 ccgcccttcc ctgccgtggc aggcctgtgg cagaaaccca gtattctgaa taacgttgaa
    18721 acactggcca atgtcggaca gatcatcctg cgcggcgcca aatggcacgc gtcgatcggc
    18781 accgagaaaa gcaagggcac aaaagtgttc gccctcaccg gcgacgtcaa caacgtgggc
    18841 ctggtggaag tcccgatggg caccaaactg ggcacgatcg tctatgacat cggcggcggc
    18901 attcccaaag gcaagaaatt caaggcggcg cagctgggcg gcccgtccgg cggctgcatt
    18961 ccggttcagc atctcaacgc ctccgtcgat tacgaaaaag tcgcggagct gggcgccatt
    19021 atggggtccg gcggtctgat cgtcatgaac gaagacaagt gcgccgtcga tatggcgcgg
    19081 ttcttcatgg acttctgtca ggatgaatcc tgcggaaaat gcaccccctg ccgcgagggc
    19141 accaaacgca tgctggaaat cctgaccaac atcacacagg gcaaaggcaa acccggcgac
    19201 atcgaactgc tcgaggaaat ggccggcgtc attaaaaacg cggcgctgtg cggcctggga
    19261 cagacggcgc ccaaccccgt tttgagcacc atccgctact tccgcaagga atacgaagag
    19321 catatttttc agcatcgctg ccaggccacc gtttgctcgg ccttgttcaa atccccctgc
    19381 cagcacacct gtccggtgga aatggacatc ccctcctata ttgcgctggt gcgcgaaggc
    19441 cggtttgagg acgcctacaa gatcctgctc cagaccaatc ccttcccgtc ggtttgcggc
    19501 cgcgtctgtg accacaaatg ccagtccaag tgccgccgcg gcaatatgga tgaaccgctg
    19561 gccatcaagt tcctgaaacg attcatcacc gacaacgcgc cgcgtccgaa aaccgacccg
    19621 gcgccggtca cccgcaagga aaagatcgcc gtcgtcggcg ccgggccggc cggcctgacc
    19681 gccgcccgcg acctcgcgct gcgcggctac aaggtaaccg ttttcgaaga gctcccgtat
    19741 gcgggcggca tgctgcgctg gggcatcccg gcctaccggc tgccgcgcaa catcctgcag
    19801 ggagaaattg acgacatcac cgcgctgggc gtggagattc gcctgaacat ccgcgtgggg
    19861 aaagacatcg ccttttcaca gttggaaaag gattttgact acttctatct cgccaccggc
    19921 gcgcacaaga gccagaaaat gcgcgtggaa ggcgaagacc tggccaatgt tttcggcggc
    19981 gtggaattcc tgcgcgactt caacaacaac gaagacaaat ggctcagggg cgaaaagacg
    20041 ctgggtaaga aagtggcggt catcggcggc ggaaactcgg cgatcgatgc ggcccgtgtg
    20101 gcgctccgcc tcggttcgga agtcaccatc ctgtaccgcc ggctgaggca ggatatgccc
    20161 gcggcggaag aagaaatcaa ggcagcggaa gaagaaggaa tcaagatcga atacctgatc
    20221 gctccgctga aaatcgacgg aaccaaaggc aaagtgagct ccatcacctg ccagcggatg
    20281 aaactgggcg actttgacaa cagcggccgc aagaaacccg cggcgatcga aggctccgaa
    20341 tttacgctgg ccgtcgacgc gatcgtggcc gccatcggcc aggttccgga tatgagcttc
    20401 atcgacaaga aggtcggcgt tgaaatcaac aagtgggact gctacacggt cggcaagggc
    20461 ttcaagtccc gcacgacgaa cccccgctac ttcgcgggcg gcgacgcgga aaccgggccc
    20521 gacacggtca ttggcgccat tgccgcgggt catcaggcgg cggatgatat cgacgcggcc
    20581 attcgccagg cgaatcatga gccggcttac gaaaagcccg cgctcgaaaa aattgacgtg
    20641 ccgctggtga tcgacgacga aacgactgaa acgccgcaga tgccgatgcc cgaaatgcac
    20701 catgccacgc gcaagatgag cttcgcggaa gtggaactgg gcttcagcaa ggaagacgcc
    20761 atgaaggaag cctgccgctg cctgcgctgc gacgcgtcgg tttaagattc ttcaatcaac
    20821 ccccctcatc aacaaagggc gattcccggt ctttcaggaa tcgccctttt tctttgcccg
    20881 gtcacgatgg gacgccgagg gatcagagag agcccagggc aatgagctcc ttgatattga
    20941 tatgccggga ctcctccacg gacaagccct cgcggatcag ctccagcaca tgcggctttt
    21001 cctccgggaa cgcgtagcct tcataggact gggagaaaag caggcctccc tgccggttgg
    21061 tccaggcgtt catattgtgg tcaaagcaga cgcgcgacgt gacgttcagg ttttcgatca
    21121 tcacctgcaa ttcttccagc cgttcgtggg gcgatgaaat atgcagcctt ccctgttcgt
    21181 attcctcaaa gagttccgtg ccctgacggg gcaccagggg acgcgaccgg atgtagtgcg
    21241 gattgatcgc gctgagcaca cgcgccgtat tctccgcgtg ccggcgccag cgctccctgc
    21301 cgcccagatc gggcatccag tactcggaaa gctgaaaacc ggcggccatc gcttttttgc
    21361 cgccttcaat ctgctcctcg ctggcaacgc ctttgcggac gattttcaaa atttcatcat
    21421 cgcccgtttc cagaccgaca tgaatgcggt ccagacccgc tttacgaatc gcgaccagtt
    21481 cctccagctt cctttgcgcc agcgtttttg agcgggtata ggaagtcacc ctggtgaggc
    21541 tgaccagcgt ttttcgcaaa tgttgaagca cttcgacaaa ttcgccgggc ctcatgatca
    21601 tactgttggc gtcctgaaga aaagccgttt tgccgccgta gtacaaccag ttgaaaacgg
    21661 atgaaaaaca gtagttttcc cgcagataag ggtcgacgct cagaatcgcc tgcagtacct
    21721 cgcggttgat gcgtccatcg ttccccgtct tgatggaaat ttcccggatt tcgtccgaga
    21781 tggctttgac ggtgtcgatg tccgccttga cgtccgctac cggccggtaa ccgaagggat
    21841 ggcctttgta catttcgcaa aacgtgcatt tattccaggg gcaattttcc gtaattctca
    21901 acaacagcga agcactgccc ccttcgctgg gcggccggta cacacctacg gcaaacgtta
    21961 atttacttat gttttcaacg ttttcccgat aagattcggg tttcgtaaaa gacgccatgc
    22021 tttttctcct tctct
//
LOCUS       DAOSRM010000027        21655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2620_length_21655_cov_112.294907, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000027 DAOSRM010000000
VERSION     DAOSRM010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 21655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21655
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2620_length_21655_cov_112.294907"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            836..1168
                     /locus_tag="PLA58_04265"
                     /pseudo
     CDS             836..1168
                     /locus_tag="PLA58_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1334..1693)
                     /locus_tag="PLA58_04270"
     CDS             complement(1334..1693)
                     /locus_tag="PLA58_04270"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOR62043.1"
                     /translation="MQRFMMKSKIHRATVTDADLHYEGSISIDEKLMEEANMVPYEQV
                     EIYDVNNGERFSTYIIKGKRNSGTICLNGAAARKVAKGDLVIIVTYVAVDDAEAKTWK
                     PKCVLVDAKNKIKKVSK"
     gene            complement(1726..2592)
                     /gene="panC"
                     /locus_tag="PLA58_04275"
     CDS             complement(1726..2592)
                     /gene="panC"
                     /locus_tag="PLA58_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOR62044.1"
                     /translation="MDNLQVIESVKAMQSHCERLRLAGKKISFVPTMGYFHEGHLSLM
                     KEARRTADHVVVSIYVNPTQFGPKEDFSKYPRDFDRDAAMAKSVGVDVIFFPPNSEMY
                     PEGYQTYVNVEKVTQNLCGLSRPGHFRGVTTVCCKLFNIVKPHSAVFGRKDYQQLAAI
                     RRMVADLNMDLEIVGMPTFREPDGLAMSSRNVYLNKEERASALTLVGSLKMAQKLYAE
                     GERNAVKIIRQAEKLITSAPFTAIDYIKICDAATLEDVLEIKGEAVMALAVKVGKTRL
                     IDNSVLGDKLEA"
     gene            complement(2776..3027)
                     /locus_tag="PLA58_04280"
     CDS             complement(2776..3027)
                     /locus_tag="PLA58_04280"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOR62045.1"
                     /translation="MIKTHRMQQYGHYRQYQKMSGILMISAWGFAMVIASFLFLWLGY
                     KLDEYMGTAPNFMFGLFFLAIFVCVMRLYHEARQRMKDL"
     gene            complement(3070..3345)
                     /locus_tag="PLA58_04285"
     CDS             complement(3070..3345)
                     /locus_tag="PLA58_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62046.1"
                     /translation="MKKTLGTIVAAAAVVLLTATFGFAEYAAAGAAQFPYFQLGCLIV
                     GGLIMVSLKRKYNKMYTAEVVGAFALYTLLMALFTNPVIDAVKNIVT"
     gene            3664..5943
                     /gene="eutB"
                     /locus_tag="PLA58_04290"
     CDS             3664..5943
                     /gene="eutB"
                     /locus_tag="PLA58_04290"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HOR62047.1"
                     /translation="MSRREFCCALAGSLAGAALLPPHLQASLQTSDPNEDLFSFMNRT
                     AGGFNRVTYRQIIGAANAFKEGDQILGVAAADETARANARRLLSNTKLGDILKHPLFE
                     DNLYASLVQNLSPDAVRKSSPMTLGQLKAFLLASPESDIHPILDGLCSDAIGCVVKLM
                     SDEDLSAVGRKIFNPLAGTRIGARGYMGARIQPNSPTDHPDDVIWQIFSGWSYATGDV
                     VLGTNPVSSLPESVAALETALADIRRTFGLEDILPHSVLAHIDIQAEVEKRQPGTTGV
                     WFQSLAGSDKANATFDISLEKMLAYASQRTGLYGFYFETGQGADFTNGSGQGTDMVIH
                     ESRKYGFARLLKKKVAEAQQKAGRTPAPWVHVNDVAGFIGPEVFRTREQLVRCCLEDI
                     VMGKLHGLTIGLDICSTLHMDVSLDDLDWCQDRIMPANPAYLMALPTKNDPMLGYLTT
                     AFQDHVRLRKQFGYKVNDDMWRFFQKLGVIDAAGNPTSRFGQPLWVWYAYRRAKGDRR
                     SRTDIFQEGRKKMREVRQRGVYLAEGFNGRPWKMEAKLDREIRALYADSKKCIWVELE
                     PSFIATLPNGVLLQSRSKDRTDYILHPQTGEELDEPSLRSLHKLKVKRGDAVQVQIIV
                     SDGLNAHAVMDPEHLAPYLSHLEKGLREKHLTVAPELLVLRGGRVRAGYRIGEVLFGN
                     APDAPGHKAIIHIIGERPGTMHHTFSAYITAPAAAVWTKTGIDHNITRVVSGVADTAL
                     HPDLAAGETVKILDDLWKE"
     gene            5969..6319
                     /locus_tag="PLA58_04295"
     CDS             5969..6319
                     /locus_tag="PLA58_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62048.1"
                     /translation="MKKVATFLFAVCFIFCFSSGMSVAGESSSSGKSVTKPVTETAQE
                     VKQGTVKIYKESKDAVIRDAKEMKEEFPKDVKKAKKEALQKAKKVKKGASSEWKEIRD
                     NMANPKLTTTPEKK"
     gene            6398..7480
                     /locus_tag="PLA58_04300"
     CDS             6398..7480
                     /locus_tag="PLA58_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOR62049.1"
                     /translation="MISVIGVSASYAQTKAPPSAGQAILEAIRINEPLDFCGEPVPLD
                     DPDVRERLERELLVSLDNSDDIILWLKRANRYFPDVEKVLKAQSLPDDLKYITIAESS
                     LRPLAFSNKGAAGYWQFIEGTGKKYGLEISGDIDERRNVHRATQAAVTYLKDLHDLFG
                     SWTLAAAAYNMGEDGLKSEMLIQKVNNYYQLYLNQETQRYVFRILAAKIIMSNPERFG
                     YSLSKSDLYAPRHSETVEIKASEPVPLHIIAGAANTYFKIIKDLNPQIKYYSLPAGTY
                     SIHIPAGQAAGFHERYEKLLADWIALKNDSVYVVKKGDTLTGIAKQFNVPYRALTIWN
                     NVGGRKISPGDKLYIFSDSLKRESKP"
     gene            7477..7884
                     /locus_tag="PLA58_04305"
     CDS             7477..7884
                     /locus_tag="PLA58_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOR62050.1"
                     /translation="MNKRILKSGRLPASGPYSAAVEAGGLVFLSGQLPINPATGEVVT
                     AIGPAARQVLANLQTVLEENDLSLADVVKTTIFLTNMENFAVVNEIYAGFFPDQPPAR
                     STVEVSALPKNVPLEIEAVAVRKQGGAHESSRS"
     gene            7865..8929
                     /locus_tag="PLA58_04310"
     CDS             7865..8929
                     /locus_tag="PLA58_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOR62051.1"
                     /translation="MKVLALNSSSRTGDVSKTEIILGDLIQGMREAGAEVEVVELRRK
                     KIRYCIGCFTCWTKTPGVCLHKDDMTREIFPKYRDSDLVVLATPLFHYTVNAIMKTFI
                     ERTLPIALPFFEKQGEATRHPYRHKAPHVVVLSVAGFPEESVFDALKFYARFLFRDHL
                     AAEIYRTSSEMFLHSTGSRKVSEVREALIQGGRELVGSMKISPETLKRIHKPITTFEE
                     MAPLGNLMWQTCIDEGVTLGEAQQKKIIPRPDSIENFLMLMRFAFKARKAGDTKMAVQ
                     FNFSGQAAGECYFIVENGIFRTGVGKPDHPDLVIDSPFEVWMDIMTQKADGQKLFMEQ
                     KYSAQGDITVLMRFKDLFGN"
     gene            complement(9024..9100)
                     /locus_tag="PLA58_04315"
     tRNA            complement(9024..9100)
                     /locus_tag="PLA58_04315"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9064..9066),aa:Pro,seq:tgg)
     gene            complement(9105..9701)
                     /locus_tag="PLA58_04320"
     CDS             complement(9105..9701)
                     /locus_tag="PLA58_04320"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOR62052.1"
                     /translation="MRIVLATKNEGKVREIAQMMEGAGIEMLSLNHFAALPEIVEDGT
                     TYLENALKKARTVSEFLGETVLADDSGLQVDALGGEPGVYSARYAGEGATDEENNAKL
                     LAKLKEVPGETRTASFFCALVLYQPGGLYHCFEADWRGRIIDEGRGANGFGYDPIFLD
                     PNFNRTAAELPPEIKNKVSHRGQAFAKFKDWLAAGRLG"
     gene            complement(9698..10429)
                     /gene="rph"
                     /locus_tag="PLA58_04325"
     CDS             complement(9698..10429)
                     /gene="rph"
                     /locus_tag="PLA58_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOR62053.1"
                     /translation="MFRRNADRKYDELRPITIANRYLKNVPGSVMVEFGNTKVLCAAS
                     LDEKTAPFLKNTGRGWLTAEYAMLPMSTSTRSARESARGKVGGRTHEIQRLIGRSLRA
                     VTDLNAFGERTIYIDCDVIQADGGTRTASITGAFVALVDLFKDMKQQGIIREIPVADF
                     VSAVSVGMVENQILLDLEYEEDSRADVDMNFVMTGSGLFIEVQGTAEHAPFGKDQLDA
                     MTALAAGGIRQIIACQKEITGDWRS"
     gene            complement(10437..11060)
                     /locus_tag="PLA58_04330"
     CDS             complement(10437..11060)
                     /locus_tag="PLA58_04330"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOR62054.1"
                     /translation="MGTAGYAQTARHVVVIDAAHGGDDAGVLGVDGAAEKDVTLAVAL
                     ALQKELSTGGGLEVLLTRNTDRTLSLEERKKFIQKASPALVLSLHANAGFGKIATGFE
                     LYYPGFAKARAANKAAPAAGTPDNRHLNDTVKLARLVQKNLDSLFPRQGRGLREAGIP
                     LAEGLAAPVLVVEMGFLTNADGKKKLTSGKTQAEIAAALAKSIRGFF"
     gene            complement(11224..13368)
                     /locus_tag="PLA58_04335"
     CDS             complement(11224..13368)
                     /locus_tag="PLA58_04335"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOR62055.1"
                     /translation="MLRINDILDKAQTYLTSEELEMIQKAYIYSATVHQGQVRLSGEP
                     YLTHPMEVAGILVDMKMDSATIISGLLHDTIEDTLTTREQIEQEFGKDVAFLVSGLTK
                     LSRISFGSQEERQAENFRKMILAMSSDIRILMVRLADRIHNMRTLQYQTAQRKIYIAR
                     ETMDVYAPLANRLGINWMKMELEDLSFRYLSPDAFNEIASRVAKSQESRNRYTEEVKG
                     IIFRELEKVSIQGEVEGRAKHLYGIYRKMHEQHINFDEVYDVIAFRIILDSSADKSCY
                     EALSIVHALWKPVPGRFKDYIAMPKANNYQSLHTAVIGPYGERVEIQIRTRAMHEWAE
                     KGIAAHWRYKEGRAFQDHEDDQIKKLRETLEIQQDIKNPREFMSNLKMALFPDDVYVF
                     TPHGDVKSFPKGATPIDFAYSVHTDVGHQCTGAKVNRNIVPLRYQLQNGDMVEIITQS
                     GHHPSKDWLKFVVTSRAISKIRNWINAEEKNRSLALGKDLLEKEFKRNNLKFATYLKS
                     EEIRKLLKEYSLLTLDDLISQVGCGRISPRQVVNHFVVQETPKETEKLPEKEKKKPAA
                     APSLGISLTGIDDVMVRFAKCCNPIPGDEITGYISHGRGITVHTQNCPHLQKLDIERI
                     VEVNWDLRQKNAYPVHIKVVCTDRKGVLTEVSSVITSLDVNISFAQVETTDMIASCSF
                     IIDVYDTQQLNAVFSAIRQLKIVRSIERIRKA"
     gene            complement(13394..15118)
                     /locus_tag="PLA58_04340"
     CDS             complement(13394..15118)
                     /locus_tag="PLA58_04340"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOR62056.1"
                     /translation="MRYSEMHLPTGREIPSDAEVVSNQLMIRAGMIRKLTSGIYSYLP
                     LGYRVIRKVERIVREEMNKAGAQEVHLPMVQPAELWQESGRWTYYGKELLRFRDRNDR
                     DYCLGPTHEEVITDLVRHDIKTYRQLPRNLYQIQTKFRDEVRPRFGVMRCREFGMKDA
                     YSFDADEAGAEKSYEKMFAAYNAIFRRCGLRFRPVEADSGSIGGSFSHEFMVLAESGE
                     DAIAFCEKCNYAANLEKAEIARPEKAAPIASDRPALESVETPNVRTIEEVSAFLNVSP
                     RQIVKTLIFNADGKACAVLIRGDHEVNEIKVKNILGAAELELADDEMIFKATGAPRGF
                     AGPVGVKVRVIADYSILDLVDMVTGANREDYHLKNVNLGRDFTVESFADLRVVQPGDA
                     CPRCGGEIKFARGIEVGHVFKLGTKYSKAMKAVYLDRDGKEKTMIMGCYGIGIGRTVA
                     ACIEQNFDQNGIIWPMPLAPYAVIVTPVNINEPDVMKAAGEIYEELLAADVEVILDDR
                     DERAGVKFKDADLIGIPLRIVVGSKNLAQGQVELKIRKTGDSRLYPKSDIVSEVRTII
                     AQELKTAG"
     gene            complement(15128..16198)
                     /gene="ispG"
                     /locus_tag="PLA58_04345"
                     /gene_synonym="gcpE"
     CDS             complement(15128..16198)
                     /gene="ispG"
                     /locus_tag="PLA58_04345"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOR62057.1"
                     /translation="MEEASIFRRKSRIIRIGSVRIGGDEPIAVQSMTNTDTRNVAATV
                     AQINALERAGCEIARVAIPDADAVRALPDIKKGISIPLIADIHFHAQLAIDAVKNGAD
                     GIRINPGNMAKERIAEIVRTAKEHQTAVRIGVNAGSLQKDLLAEYGVSARALYESALR
                     SVSLFEDLGFEQIKLSLKSSDVPMMVEAYRLIAAATDYPLHLGVTEAGTLVDAAVKSA
                     LGIGILLAEGIGDTLRVSVTGDPVSELPIAYSILRALKIRKVGAEIISCPTCGRCEID
                     LAGLVSQVEQALAGRKNYIKVALMGCVVNGPGEAAEADIGIAGGRGFGMLFKKGVACK
                     KVEEKDFVPVLLEEIRSLDNEP"
     gene            complement(16369..17772)
                     /locus_tag="PLA58_04350"
     CDS             complement(16369..17772)
                     /locus_tag="PLA58_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOR62058.1"
                     /translation="MGGIFGVVSEDSCVQTLFYGTDYHSHLGTEHGGMAVYGPRGFYN
                     TIHEISQAQFKSRFVDDYRKMEGHSGIGAIDDESPQPLIFRSKFGVYAVVATGLITNK
                     SELVGELLNEGASFTEVAGGGVNNSEIVASLINRGDNIVEGIASMRGIIEGSICVLVL
                     TAEGIYAARDKVGRFPLALGRSKDPNRKSYVVATEASSFENLGYELQRFLGPGEIALL
                     TPDGVRQLKPEGSQKKICSFLWIYTGSPSSIYDGVGVESTRERCGRYLARRDHVKVDF
                     VAGVPDSGVGHAVGYAMESGIPYRRPLVKYTPGYGRSYTPPTQEIRDLVATMKLVAVR
                     DVIFGSRMVICDDSIVRGTQLKNLTVKKLWDNGAKEIHIRPACPPLMYPCIYASSTRT
                     TSELVCRKAIRAMEGRDLEDISAYLDTTSAKHAKMIDWIRQDLNVTSLRYMTIEDMIA
                     AIGLPADQLCLHCWLGK"
     gene            complement(17797..18990)
                     /locus_tag="PLA58_04355"
     CDS             complement(17797..18990)
                     /locus_tag="PLA58_04355"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOR62059.1"
                     /translation="MKLASRVAKVKPSETLAITAKANALRAQGRDVIGFGAGEPDFDT
                     PDHIKQAAIRAIEAGFTKYTPVGGTDELKDAIIAKLKTDNRLEYKRSQVVVSCGAKHT
                     LYNLAQALFEEGDEVIIPAPYWVSYPDIVVLAGGTPVILNTLEKDGFKMKPEQLKASI
                     TGRTRAVIFNGPSNPTGAAYSPDELKALAAVLADTDIFAISDDIYEKILYADFPFANL
                     AMAEPRLKDRTIVVNGVSKAYAMTGWRIGYAAGPEEIIGAISKIQSQNTSNPTSISQK
                     ASVAALAGDQQVVSMMVAEFKKRKDYIVDALNDIPGIFCPMPEGAFYVFPNVSALYGR
                     SYEGKKISNSTEFIEYLLDGANVATVPGVAFGSDDHIRLSYATSLKNIEEGVRRIKDA
                     VAKLG"
     gene            complement(18987..19511)
                     /gene="coaD"
                     /locus_tag="PLA58_04360"
     CDS             complement(18987..19511)
                     /gene="coaD"
                     /locus_tag="PLA58_04360"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOR62060.1"
                     /translation="MTQEKTGYKIAIYPGSFDPITNGHVDIIKRGSRIFDKIIVLVAY
                     NPRKSFLFSMEERCQLIREIFKGCECIQVDSHDGLLIDYLQKSGASTILRGMRALSDF
                     EYEFQMALMNRRQTKNVETVFLMSGFRWFFTSSNIIKEVASLGGTVRGLVPDNVYQRL
                     LEKYPNKKTTGGRL"
     gene            complement(19508..20062)
                     /gene="rsmD"
                     /locus_tag="PLA58_04365"
     CDS             complement(19508..20062)
                     /gene="rsmD"
                     /locus_tag="PLA58_04365"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOR62061.1"
                     /translation="MRITGGKFKGRTLHFPSRSSSRPTTDFLREALFNLLGPQTDRSF
                     LDLFAGSGSVGLEAASRGAKQIVFVEKSGKLIGVLRENVSLCGCSEQARIIHDGVASA
                     LCVLYDQPGRFDVIFADPPYGQGFIGETLGALRNCPVIREGGILVLQHSVREPLPELP
                     KPWLVADQRKYGENLLTFIRMDGA"
     gene            complement(20095..20877)
                     /gene="pssA"
                     /locus_tag="PLA58_04370"
     CDS             complement(20095..20877)
                     /gene="pssA"
                     /locus_tag="PLA58_04370"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOR62062.1"
                     /translation="MNSGRHIRNTRLKKGIYVLPNLITTASLFMGFYSIIASIQEKFF
                     VAAIAILVSMVFDGLDGRIARITNTTSKFGAEYDSLADLIAFGVAPSLLAFMWAMSSY
                     GKLGWMAGFLFVTCGALRLARYNIQIGLIDSKVFNGLPIPAAAAVIATTVIFVNDVMG
                     IEGIFHNPYVMICVVILAFLMVSNIKYYSGKDMKLFARMPFMTFLVVVGILLVIIYKP
                     EVTIFVITVGYALSGPVWWLCKVINKKHGTETDAKDLPGGEK"
     gene            complement(20874..21515)
                     /locus_tag="PLA58_04375"
     CDS             complement(20874..21515)
                     /locus_tag="PLA58_04375"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOR62063.1"
                     /translation="MKHKSIIVAEGFPFIVLSLVFTGFAAFFGVAWLTILLAALTIFI
                     IAFFRNPQRHFQAAEKLVISPADGKVIKMEEVDVRGAMAGRFKKISIFMNVFNVHVNR
                     APYEGTIEKISYHPGKFVSANLDKASTENERNEVLIRTEDGRAIWTVQIAGLIARRIV
                     CWIREGETLAKGERFGMIRFGSRVEVFLPADSRVVVRLNDKVRAGQTPLGYLS"
BASE COUNT         4827 a         6152 c         5707 g         4969 t
ORIGIN      
        1 gtgacactcg cttcgcgtgc cgctccttct caatccactc gtacaacgat tccggcacgt
       61 cccgaatgag aatctgtctt tttggcattt cgctcacctc cggtaaaatt gataacattg
      121 atatcaaata tccctgacga tgtcaatcgg gtgcgtccag cgattacttc gtcaaggaga
      181 ttctggaaac gggaatacgc atcgtataaa agcattgcaa aaagaggcta aaaaacaacg
      241 ggaagttaag caggaaacag cgtgcctgtg tccattagag caatcatacg gattgctcca
      301 gaatattccg gtttaagctg caaccagcta agaactgata tttcaagacc tgaccccaag
      361 cttccgcccc aggcgcacct gctcttcaat ccagggacga tgttcttcgc aggctgaccg
      421 ggcgtgagcc ggcatgggtt catcccgcgt agatggcgcc tcaaaagccg gtggccgggg
      481 tgggggattt cgatcggtcg gcgcttgttg tttgtgtttc tttattctta tccgccatcc
      541 ggcatacttg cggatggtct tacgatccac acgtactttc cgcccaatct cccgctgact
      601 gacaccattg gtaagtaacg taatgattga tgttttcttg ttcggttgca aaacgttcat
      661 cctttctctt cctcccattt tctgtgcaaa aagtgggagt ctgtaacgtt ctgccaaccg
      721 tttgtgaagg ggcttttgat cgcctgtttt caggcgctaa aatcaaccgg tgggtggggg
      781 attttcaagt ggccacaggt gggggatttt gggtggccgc cggggctatg cgcacgcgac
      841 aaaggttcga ctggttatgg ataatttgaa cactcacagc atcgcctcac tttatgaaac
      901 attcgaaccc aaggaagcca ggcgcttggc ggaacgactg gatattcatt acacgccgaa
      961 acacggcagt tggttaaaca tggcggagag tgaactcggc gtcctgaaag gacagtgcct
     1021 caattgccgg atacccgata tggtaagcat gcaaacccaa gtggccgcat ggaataaaaa
     1081 tcgaaatagc agtgcgaaaa aaatatcatg gcaatttaca acgtccgatg ctcgcataaa
     1141 attaaaacgt ctttatccga atttataggc gttacatggt actagaacag taaagaattt
     1201 aatgaagctg ctgcattatc aaaatatggt cagggtctgt aaacgcatga gcgtcccgca
     1261 atcggaacgc tgtccgtaat gggaataaca aggaacatcc ctgagaaatg gaatgactac
     1321 aaatggaaac gttttatttg gagacttttt taattttgtt tttcgcgtcc acgaggacgc
     1381 atttgggctt ccaggttttg gcctccgcat cgtccaccgc cacgtaagtg acgatgatga
     1441 ccaggtcgcc cttggcgact ttgcgggcgg cggcgccgtt gagacagatc gtgccggagt
     1501 ttcgcttgcc cttgatgata tacgttgaaa acctctcccc gttgttgaca tcgtatatct
     1561 ccacctgttc atagggcacc atgttcgctt cttccatcag cttctcgtca atcgaaatgc
     1621 tgccttcata atgcagatcg gcgtccgtta cggtggcgcg atggattttc gatttcatca
     1681 taaatctctg catggcttct tttctctccc ctgtcccaaa attttttacg cttcaagctt
     1741 atcgcccaaa acggaattat caatcagccg cgttttcccc actttgacgg ccagggccat
     1801 cactgcctct cccttgattt ccaggacgtc ttccagggtt gccgcgtcgc atattttaat
     1861 ataatcaatg gcggtaaagg gcgcgctcgt gatgagcttt tccgcctgcc ggatgatttt
     1921 gaccgcgttt ctttcgccct cggcataaag tttctgcgcc atcttcagtg aaccgaccag
     1981 cgtaagcgcc gacgcccgct cttctttgtt cagataaaca ttgcgcgaac tcatggccag
     2041 gccgtccggt tcccggaagg tcggcatgcc gacgatttcc agatccatgt tcaggtcggc
     2101 aaccatccgc cggatggcgg ccagttgctg ataatctttc ctcccgaaaa cggcgctgtg
     2161 cggtttcacg atgttgaata gtttgcagca gacggttgtg acgccgcgga aatggccggg
     2221 ccgggataag ccgcacagat tctgcgtgac cttctccaca ttgacgtagg tctgatagcc
     2281 ttccggatac atttcggaat tcggcgggaa aaagatgacg tcaacgccga cgctcttcgc
     2341 catggccgca tcccgatcaa aatctctcgg gtatttggaa aaatcttctt tggggccgaa
     2401 ctgggtggga ttgacgtaaa tgctgacaac gacatggtct gcggttctgc gggcttcctt
     2461 cataagagac agatgacctt catgaaaata gcccatggtc ggaacaaagg atattttctt
     2521 gccggcaagc cttaagcgct cgcaatgcga ctgcattgct ttgacggatt caatcacttg
     2581 cagattgtcc ataaaaaaag cctccggtcg caagacgaga ggctgaattt ttttctagtt
     2641 ttccttccgt cccagtcctt gcggatccaa gcgtcggggg acactaacaa taaaaataaa
     2701 tgcttgtcaa gaaaaaaaac ccccttggac cgacggttcc aagggggcga tactattgac
     2761 agatcatgtt ttcggttaca aatccttcat ccgctgccgg gcctcgtgat acagccgcat
     2821 cacacaaacg aagatcgcca ggaaaaacaa accgaacatg aaattgggcg ccgtgcccat
     2881 gtactcatcc agcttgtacc ccagccagag gaacaggaag gaggcgatca ccatcgcaaa
     2941 gccccaggcc gaaatcatca ggatcccgct catcttctga tactgccggt aatgtccgta
     3001 ttgctgcatc ctgtgtgtct tgatcatggc cctgtcctcc tttctgaaag tctctcttaa
     3061 tccttaacct caggtcacaa tgttcttcac cgcatcaatc accggattgg tgaagagcgc
     3121 catcagaagg gtgtaaagag cgaaggcgcc gacgacttcc gccgtgtaca tcttgttata
     3181 cttcctcttc aggctgacca taatcagtcc gcccacaatc aggcagccca gctgaaaata
     3241 cgggaattgg gccgcccctg ccgccgcata ctctgcaaat ccaaaggttg ccgtcaaaag
     3301 aaccactgcc gccgccgcta cgattgttcc taaggtcttc ttcatgatct gtttcctcct
     3361 tcaagggttt ttttgtttct ttcgccttat tcctaatgaa aaacccgtgc caaaaatgca
     3421 gaccaggagg cgatatttta taacacaatg atattattaa gcattatgtg tgatacctac
     3481 agggtttaca ccgtgtaaac caacctttcc ggcgtttttc tttcgatgaa atgttcaaca
     3541 gacttgaacg aagcgtaaaa aagtattagt cattgaccaa cgaaaattct tgcaggaaag
     3601 cggtccatcc atcaggctcg gataatgaag atcagtccta tcacggaaag gagaggcggg
     3661 cgcatgagca gaagggaatt ttgttgcgcc ctggccggtt ctctggccgg cgcggccttg
     3721 cttccgccgc atttgcaggc gagccttcag acctcggatc ccaacgaaga tcttttttct
     3781 ttcatgaacc gcacggcagg cgggtttaac cgcgtcacct accggcagat catcggcgcc
     3841 gccaatgctt tcaaggaggg tgatcagatt ctgggcgtgg ccgctgccga cgaaaccgcc
     3901 cgggccaatg cgcgccggct tctgtccaat acgaagctcg gggatatttt gaagcatcct
     3961 ttatttgagg acaacctgta cgccagcctg gtccaaaatc tttcccccga cgccgtccgg
     4021 aaaagcagtc ccatgaccct gggccagttg aaggcgtttc tgctcgcgtc gcccgagtcc
     4081 gatattcatc ccatcctcga cggcctgtgc agcgacgcga tcggctgcgt cgtgaagctc
     4141 atgtccgatg aagatctttc tgccgtggga cggaaaatct tcaacccgct ggccggaacc
     4201 aggatcggcg ccagaggcta catgggcgcc cgcattcagc ccaattcgcc caccgatcac
     4261 ccggatgatg taatctggca gatcttttcc ggctggtcgt atgcgaccgg cgatgtggtc
     4321 ctcggaacca acccggtcag cagtcttccg gaatccgttg ccgccctgga gacggcgctt
     4381 gccgatatcc gccgcacctt cgggctcgaa gacattctcc cgcacagcgt tctggcgcac
     4441 attgacattc aggcggaagt ggaaaaaaga cagccgggta caaccggcgt gtggtttcaa
     4501 agtctggcgg gcagcgacaa agccaacgcc acgttcgaca tttcgctgga gaagatgctg
     4561 gcctacgcat cgcagagaac aggcctgtac ggattttatt ttgaaaccgg acagggcgcc
     4621 gacttcacca acggcagcgg tcagggcacc gacatggtca ttcacgaatc gcgcaaatac
     4681 ggcttcgccc ggctgctcaa aaaaaaggtt gccgaagccc agcaaaaggc gggacgcacc
     4741 ccggcgccgt gggttcatgt caacgacgtg gccggattta tcggcccgga agtttttcgc
     4801 acccgcgaac agcttgtgcg ctgctgcctg gaagacattg tcatgggcaa gctgcacggc
     4861 ctcaccatcg ggctggacat ctgctccaca ctgcacatgg atgtttctct tgacgatctg
     4921 gactggtgcc aggaccggat catgcccgcc aatccggcct acctcatggc gctgccgacc
     4981 aaaaacgatc cgatgctggg ctatctgacc accgcctttc aggaccatgt gcgcctgcgc
     5041 aaacaattcg gatacaaagt caacgacgac atgtggcgtt tctttcagaa gctgggggta
     5101 atcgacgcag cgggaaatcc cacttcccgt ttcggtcagc ccctttgggt ctggtacgca
     5161 taccggcggg caaaaggaga ccggcgctcc cgcacggaca tttttcagga aggccgaaaa
     5221 aaaatgcggg aagtccgcca acgcggcgtc tatctggcgg aaggctttaa cggccggccc
     5281 tggaagatgg aagcgaaact ggatcgggaa atccgcgcgc tgtacgcaga ttccaaaaaa
     5341 tgcatctggg tggaactgga gccgtctttc attgcaaccc tgccaaatgg cgttttactg
     5401 caaagccgtt caaaggacag aacggattac atcctgcatc cgcagacggg agaagagctg
     5461 gatgagccgt ccctgcggag cctgcataag ttgaaggtca aaagaggcga cgccgttcag
     5521 gtgcaaatca tcgtgtcgga cggcctcaac gcccatgccg tcatggaccc ggaacatctg
     5581 gccccgtatc tgtcgcatct agaaaagggc ctgagggaaa aacacctgac ggtcgcccct
     5641 gaactcctgg tgcttcgcgg cggacgcgtg cgggcggggt atcgcatcgg agaagtcctg
     5701 ttcgggaacg cgccggatgc tcccgggcac aaagccatta ttcacatcat cggcgagcgc
     5761 cccggcacca tgcatcacac attttccgcg tatatcacgg cgccggccgc tgccgtctgg
     5821 accaagaccg gcatcgacca caacatcacc cgggtggtct ccggcgtggc ggacacggcc
     5881 ctccaccctg atcttgccgc cggagaaacg gtaaaaatac tcgacgatct ttggaaagaa
     5941 taaactattt tttaaaggag aaaataaaat gaaaaaagtt gccacctttt tgtttgccgt
     6001 ctgctttatt ttttgcttca gcagcggcat gtccgtcgcg ggagaatctt cgtcatccgg
     6061 caaaagcgtc acgaaaccgg ttacggaaac ggcccaagag gtcaagcagg gaacggtaaa
     6121 aatttataag gaaagcaagg acgccgttat acgggacgcc aaagaaatga aggaagagtt
     6181 tcccaaagac gtgaaaaaag caaaaaaaga ggctctccag aaagccaaga aggtcaagaa
     6241 aggagcttct tcggaatgga aggagatccg cgacaatatg gcgaatccga aactgaccac
     6301 cacacccgaa aaaaagtaat ccgggaaaac tctattccag ggagaatttg ttttgaagat
     6361 acggtcttac gcacggtttc ttttaagcgc cttgcttttg atcagcgtaa ttggcgtgtc
     6421 ggcaagttat gctcaaacca aggcgccgcc ctccgccggg caggccattc tggaagccat
     6481 ccggatcaat gaaccgctgg atttttgcgg tgaaccggtg ccgctggacg atcccgatgt
     6541 ccgcgaacgc ctggagcgcg aactgctggt gtctctggac aacagcgacg acatcatcct
     6601 ctggctgaag cgcgccaacc gctatttccc cgacgtcgaa aaagttctga aggcgcagtc
     6661 gctccccgat gatctgaaat atatcactat cgcggaaagc tccctccggc cgctggcctt
     6721 ctccaacaaa ggcgctgcgg gatactggca gttcatcgaa ggcacgggga aaaaatacgg
     6781 tctggaaatc agcggcgaca tcgatgaaag aagaaacgtt cacagggcca cgcaggcggc
     6841 cgttacctat ctcaaagacc tccatgatct ttttggatcc tggacgctcg cggcggcggc
     6901 ctacaacatg ggggaagacg gtctgaaatc agagatgctg attcaaaaag tcaacaacta
     6961 ctatcagctt tacctgaatc aggaaacgca gcggtacgtc ttccggatcc ttgcggcaaa
     7021 aatcatcatg tccaatccgg aacgattcgg gtattcgctt tccaaatcgg acctctacgc
     7081 tccccggcac agcgagacgg ttgagatcaa agcctccgag cccgtgcccc tccacatcat
     7141 cgcaggggcg gccaatacgt atttcaaaat catcaaggat ctcaacccgc agatcaaata
     7201 ttattccctg cccgccggaa cctattcgat tcatattccc gcgggacagg cggcaggctt
     7261 tcatgagcgt tatgagaaat tgctggcgga ctggatcgcc ctgaaaaacg attccgttta
     7321 tgtcgtgaaa aagggggata cgctgacggg catcgccaaa caattcaatg taccttatag
     7381 agccctgacg atctggaaca acgtcggcgg cagaaagatt tcccccggcg acaagcttta
     7441 tattttttcc gattccctta aaagagagtc gaagccgtga acaagcgaat ccttaaatca
     7501 ggccgattgc cggcttcggg cccctactcc gccgccgtgg aagcgggcgg tctggttttt
     7561 ctttccggtc agttgccgat taacccggcg accggggaag tcgtcacggc catcgggccg
     7621 gcggcccgcc aggttctggc caatcttcaa accgttcttg aagaaaacga cttgtcactt
     7681 gcggatgtgg tgaagacaac cattttttta accaacatgg aaaactttgc agtagtcaac
     7741 gaaatttacg ccggcttttt cccggatcag ccgccggcgc gttccacggt tgaagtgtcc
     7801 gcccttccca aaaacgtgcc gctggaaatc gaagccgtag ccgtcagaaa acaaggaggc
     7861 gctcatgaaa gttctcgctc ttaattccag ctcccggaca ggggacgtca gcaaaaccga
     7921 aatcatcctg ggggatctga tccaggggat gcgcgaagcc ggcgcggagg tggaggtcgt
     7981 cgaactgcgc agaaagaaaa tccggtactg catcggatgc ttcacctgct ggaccaaaac
     8041 cccgggcgtt tgccttcaca aagacgatat gacccgggag atattcccga aataccggga
     8101 cagcgatctc gtcgtcctgg ccacccctct ttttcattac accgtgaatg ccatcatgaa
     8161 aaccttcatc gagcggacgc ttcccatcgc gcttcctttt tttgaaaagc aaggcgaagc
     8221 gacccggcac ccttaccgcc acaaggcgcc gcacgtcgtt gtcctttccg ttgccgggtt
     8281 cccggaagaa agcgtctttg acgcgctgaa attctacgcg cgctttctgt tccgcgatca
     8341 cctcgctgcg gaaatctacc ggacatcctc tgaaatgttt ttgcattcga ccggcagcag
     8401 aaaagtctcc gaggttcggg aagcgttgat tcagggaggc cgggaactgg ttgggtccat
     8461 gaaaatatcc ccggaaaccc tgaaaagaat ccacaagccg atcaccacct ttgaagagat
     8521 ggcgcccttg ggaaacctga tgtggcaaac ctgcattgac gagggtgtga ccctgggcga
     8581 agcccagcag aaaaaaataa ttccgcgacc cgattccatc gaaaacttcc tgatgctgat
     8641 gcgctttgcc ttcaaagccc gcaaagccgg agacacgaag atggccgtgc agttcaattt
     8701 ttccggacag gcggcggggg aatgttattt tatcgtcgaa aacggaatct tcaggaccgg
     8761 cgtgggaaag ccggatcatc ccgatctggt cattgattcg cccttcgaag tctggatgga
     8821 tatcatgacc caaaaagcgg acggccaaaa gctgttcatg gaacagaagt attccgcaca
     8881 gggagacatc accgtcctca tgcgctttaa ggatctcttc ggcaattgag gcaatggaca
     8941 aaatttttag tccttgttgc gtccacaagc gatttttcaa agaaaagggc ttacgaaatc
     9001 ttccgtaagc ccttgatttt atttggtcgg ggcggcaaga tttgaacttg cgacatcttg
     9061 ctcccaaagc aagcgcgcta ccaggctgcg ctacgccccg ttttttatcc taatcggccg
     9121 gctgcaagcc agtctttgaa cttcgcgaac gcctggccgc ggtgactgac cttgtttttg
     9181 atttccggcg gcaattccgc cgcggtcctg ttgaaatttg gatccaggaa aatcggatca
     9241 tacccaaagc cgttcgcgcc ccgtccctcg tcaataatcc ggccccgcca atcggcttca
     9301 aaacagtgat agaggcctcc gggttgatac aaaaccaggg cgcaaaaaaa agaagccgtt
     9361 cttgtctccc cgggaacctc tttcaatttt gccagaagtt tggcgttgtt ttcttcgtcc
     9421 gtggcgccct cgcccgcgta gcgcgccgaa taaacaccgg gttcgccccc gagggcatcc
     9481 acctgcagac cggaatcgtc ggccagaacg gtttccccca aaaattccga aacggttctc
     9541 gcttttttca aagcgttttc cagataggtt gtcccgtcct ccacgatctc aggcagggct
     9601 gcaaaatgat tgagggacaa catttcaatg ccggcccctt ccatcatctg cgcgatttcc
     9661 cggaccttgc cctcattttt cgttgccaaa acgattctca tgatcgccag tcgcccgtga
     9721 tttccttctg gcaggcaatg atttgccgga tcccgcccgc ggccagcgcg gtcatggcgt
     9781 ccaactggtc tttgccgaac ggggcgtgtt cggccgtgcc ctgaacttcg ataaaaaggc
     9841 ccgagccggt catgacaaaa ttcatatcca cgtccgcccg tgaatcttct tcatattcca
     9901 ggtccagcaa aatctgattt tccaccatcc cgacactgac ggccgaaaca aaatccgcaa
     9961 cgggaatttc ccggatgatc ccctgctgct tcatgtcctt gaaaagatcc accagggcga
    10021 cgaacgcgcc ggtaatggat gcggtacgtg ttccgccgtc ggcctggatg acgtcgcaat
    10081 cgatataaat ggtccgctcg ccgaaagcat tcaggtccgt gacggcgcga agcgatcgtc
    10141 cgatgagcct ttgaatttca tgcgtcctgc ctccgacttt tccgcgcgcg gattcgcgcg
    10201 ccgaacgagt ggacgtggac atgggcagca tggcatattc cgccgtcagc cagccccggc
    10261 cggtattttt taaaaaaggc gctgtttttt catccagcga ggcggcgcag agcactttcg
    10321 tgttgccgaa ctcaaccatc acggagcccg gaacattttt caaataccgg ttggcaatgg
    10381 taatcggtcg cagctcgtca tattttctgt ccgcgtttct cctgaacaca ggcctcctaa
    10441 aagaatcccc tgatgctttt cgccagcgcc gcggcaattt ccgcctgtgt ttttccggag
    10501 gtcagcttct tttttccatc ggcgttcgtg agaaaaccca tctccacgac cagcacgggc
    10561 gctgccagtc cctccgccag cggaatcccg gcttcccgga gcccccggcc ctgccgggga
    10621 aaaaggctgt ccagattttt ctgaaccaga cgcgccagct tcacggtgtc gttcaaatgg
    10681 cggttgtccg gcgtgcctgc ggccggagcc gccttgtttg cggcgcgggc cttggcaaag
    10741 ccgggataat acagttcaaa tcccgttgca attttgccga agccggcatt ggcgtgcagg
    10801 ctcaacacca gggccgggct tgctttttga atgaattttt tgcgttcttc cagcgacaac
    10861 gtccggtccg tattgcgggt cagcagaact tccagaccgc ctcctgtgga cagttccttc
    10921 tggagggcca gcgcaacggc aagcgtcacg tccttttcag cggcgccgtc aaccccgaga
    10981 actccggcgt cgtcgccgcc gtgcgccgcg tcaatcacga cgacgtgcct tgccgtctgg
    11041 gcataccccg ccgtccccac aaagcacaac gtcaaaaccc aaaaaaagac aacggtacat
    11101 ttctgccatg atggaaacat ttttttcacc taacgacgtt cttccgcgcg ccacacggag
    11161 ttgggcctgc ttatagcaga gtccgatggt ttgtcaacag acaaatgggg gcgcgacagg
    11221 tcatcaggcc ttgcggattc tctcgatgga acgaacgatt ttcaactggc gaatggcgga
    11281 gaagacggca ttgagctgct gggtatcgta aacatcgatg atgaaactgc agctggcgat
    11341 catgtccgtc gtttcgacct gggcaaagct gatattgacg tccagagagg tgatgaccga
    11401 actcacttcc gttaatacgc cctttcggtc agtgcagacg actttgatgt gcaccggata
    11461 ggcgttcttc tggcggagat cccagttgac ttccacgatt cgttcgatgt cgagcttctg
    11521 aaggtgcgga caattctgcg tgtgaacggt gatgccccgc ccgtgcgaga tatagcccgt
    11581 gatctcatcg ccgggaatgg gattgcagca tttggcgaac cgcaccatga cgtcgtcgat
    11641 gccggtcaga ctgatgccca gcgaaggagc ggcggcgggt tttttctttt ctttttcagg
    11701 aagcttctcc gtttccttcg gcgtttcctg aaccacaaag tgattgacga cctgccgggg
    11761 cgaaatgcgt ccgcagccca cctgggaaat caggtcgtcc agtgtcaaaa gcgagtattc
    11821 cttcaggagc ttccggattt cctccgattt gagataggtt gcaaatttca ggttgtttct
    11881 tttgaattcc ttttccagca gatccttgcc gagcgcgagg gaacgattct tttcctccgc
    11941 gttgatccag ttgcggattt tggaaatggc ccgggaggtc acgacaaact tcagccagtc
    12001 cttgctggga tggtggccgg actgcgtgat gatctccacc atatcgccgt tctgcagttg
    12061 atagcgcagc ggaacaatat tgcggttcac cttggcgccc gtgcactggt ggccgacgtc
    12121 ggtatggacg ctgtaggcga aatcaatggg ggtggcgcct ttgggaaagg acttgacatc
    12181 tccgtgcggc gtgaaaacat aaacgtcgtc cggaaacagc gccattttga ggttggacat
    12241 gaattcccgg ggattcttga tatcctgctg aatctccagg gtttctctca gctttttgat
    12301 ctgatcgtct tcgtgatcct gaaacgcccg cccttccttg tagcgccagt gggcggcaat
    12361 tcctttttcc gcccactcat gcatcgcccg ggtccggatc tgaatttcga cccgttcgcc
    12421 gtaggggccg atcaccgccg tatgcagcga ctgatagttg ttggccttgg gcatggcgat
    12481 gtaatccttg aaccgccccg ggaccggctt ccacagggca tgcacgatgc tgagcgcctc
    12541 atagcagctt ttgtccgcgc tggaatccag aatgatgcga aacgcaatca cgtcgtacac
    12601 ttcgtcaaag ttgatgtgct gctcatgcat ttttcgataa atgccgtata aatgcttcgc
    12661 ccggccctcc acttcgccct ggatcgacac cttctccagt tcccgaaaga tgatgccctt
    12721 gacttcttcg gtgtagcggt ttcgcgattc ctgggacttg gcgacgcggc tggcgatctc
    12781 attgaaggcg tccggcgaaa ggtaccgaaa cgacagatct tccagctcca ttttcatcca
    12841 gttgatgccc agacgattgg ccagaggcgc atagacgtcc atggtttccc gcgcgatgta
    12901 aatcttccgt tgtgccgtct ggtattgcag ggttctcata ttgtggatgc ggtcggccag
    12961 acgaaccatc aggatgcgga tgtcggaaga catggccaga atcatcttcc ggaaattttc
    13021 agcctggcgc tcttcctgtg atccgaagga aatgcggctc aacttggtca atccgctgac
    13081 gagaaaagcg acatccttgc cgaattcctg ttcgatctgc tcgcgcgtgg taagcgtgtc
    13141 ttcaatcgtg tcgtgcagca ggccgctgat gatcgtcgcg gagtccatct tcatatcgac
    13201 caggatgccg gctacctcca tgggatgcgt gaggtagggc tccccggaca ggcgcacctg
    13261 gccctgatga acggtggccg aataaatata ggccttctgg atcatctcca gttcttcaga
    13321 ggtcagatag gtctgtgcct tatccagaat gtcgttgatt ctcagcatga agatttcctg
    13381 ccccgcctgg ggatcaaccg gccgttttca gctcctgggc gatgatcgtc cggacctcgg
    13441 aaacgatatc gcttttcgga taaagccggc tgtcgccggt tttgcggatt ttcagctcca
    13501 cctgaccctg ggccaggttc ttggacccca cgacaatcct cagcggaatg ccgatcagat
    13561 cggcgtcctt gaatttcacg cctgcgcgct cgtcccggtc gtccagaatc acttccacgt
    13621 ccgcggccag cagttcttcg taaatctccc cggccgcctt catcacatcg ggctcgttga
    13681 tattgaccgg cgtaacgatc acggcatagg gagccagcgg catcggccag atgatgccgt
    13741 tttggtcaaa gttttgttca atgcaggcgg ccacggtccg gccaatgccg atgccgtagc
    13801 agcccatgat catggttttt tctttgccgt cccgatccag gtaaacggct ttcatggcct
    13861 tggagtactt ggtgcccagc ttgaaaacgt gtccgacttc gatgccgcgg gcgaatttga
    13921 tttcgcctcc gcagcgcggg caggcatcgc ccggctgaac cacgcgcaga tccgcgaaag
    13981 attctacggt aaagtcccgc ccgaggttca cgtttttcag atggtagtct tcccggttcg
    14041 ccccggttac catatccacc agatcaagaa tcgaatagtc ggcgatcaca cggactttga
    14101 cgccgacggg tcccgcaaag ccgcgcggcg cgccggtggc cttgaagatc atctcgtcgt
    14161 cggccagttc cagttcggcc gcgcccagaa tatttttcac cttgatttcg ttgacctcat
    14221 gatcgccgcg gatcagaacc gcgcaggcct tgccgtccgc gttgaagatc agcgtcttga
    14281 caatctgccg cggcgaaacg ttcagaaaag cgctgacctc ttcaatggtg cgcacgttgg
    14341 gcgtctccac gctttccagg gccggccggt ccgaagcgat gggggcggcc ttttcgggcc
    14401 gggcgatttc cgctttctcc agattggccg catagttgca tttttcgcaa aaagcgatgg
    14461 cgtcctcgcc cgattcggcc agcaccataa attcatggga aaagcttccg ccgatgcttc
    14521 cggaatccgc ctccaccgga cggaaccgga ggccgcaccg tcggaagatg gcgttgtagg
    14581 cggcaaacat cttctcgtag cttttctccg cgcccgcctc atccgcgtca aagctgtagg
    14641 cgtccttcat gccgaattcc cggcagcgca tcacgccgaa ccgcggacgg acctcatcgc
    14701 gaaacttggt ttgaatctga tacagattgc gcggcagctg gcggtaggtt ttgatgtcgt
    14761 gccgcaccag atccgtgatg acttcctcgt gggtgggccc cagacagtag tcgcggtcgt
    14821 tgcggtcgcg gaaccggagc agctccttgc cgtaataggt ccagcggccc gattcctgcc
    14881 agagctccgc cggctgcacc atcggcagat gcacttcctg ggcgccggct ttgttcattt
    14941 cctcccggac aatccgttct acttttcgaa tgacccggta accgagcggc agataagaat
    15001 agatgccgct ggtgagcttg cggatcattc ccgcccggat catgagctga ttgctgacca
    15061 cctccgcatc ggacggtatt tcccgtccgg tgggcaggtg catttccgaa taacgcattc
    15121 ctcctcctta gggttcattg tccagggatc gaatttcctc gagcagcacc gggacaaaat
    15181 ctttttcttc cactttcttg caggcgacgc cttttttaaa caacatgccg aagccgcggc
    15241 cgccggcaat gccgatatcc gcctccgccg cttccccggg accgttgacc acacatccca
    15301 tcagcgccac cttgatgtaa ttttttcttc ccgccagcgc ctgttccacc tgcgacacca
    15361 gtccggccag atcgatttcg cagcgcccgc acgtcgggca ggagatgatt tccgctccga
    15421 ctttccggat tttcagcgcc cgcagaatgc tgtaagcgat gggcagttcc gagacgggat
    15481 cacccgtcac ggacacccgg agcgtgtcgc cgatgccctc ggccagcagt atgccgatgc
    15541 cgagggcgga tttgaccgcc gcgtccacca gcgttcccgc ttccgtcacg cccaggtgca
    15601 ggggataatc cgtggcggcg gcgatcaggc ggtacgcctc gaccatcatc ggcacgtcgg
    15661 aggacttgag cgagagcttg atttgctcaa agcccagatc ttcaaacagg gagacgctgc
    15721 gcagggcgct ttcatacagg gcccgggccg aaacgccgta ttcggccagc agatccttct
    15781 gcagcgaacc ggcgttgacg ccgatccgga cggcggtttg atgttctttg gccgtccgga
    15841 caatctcggc aatccgctct ttcgccatat tgcccggatt gatccggatg ccgtccgcgc
    15901 cgtttttgac ggcgtcgatg gccagctgcg cgtggaaatg aatatcggca atcagcggaa
    15961 tggatatccc ttttttgatg tccggaagcg cccggaccgc gtcggcgtcg ggaatcgcca
    16021 cccgggcgat ttcgcaaccc gcgcgttcca gcgcattaat ctgggccacc gtcgcggcga
    16081 cgttgcgcgt gtccgtattg gtcatggact ggacggcaat cggttcgtct cccccgatcc
    16141 ggacggaccc gatccggatg attctgctct ttcttctgaa tattgaggct tcttccatat
    16201 gccgggcgct ttcatctttg cctttggaaa ttttataaca cgggtatgtc ctgcgggcaa
    16261 tggctaattt gcagagagga ggctccttac ggattcgtct gacgaaaaaa ggcggccgcg
    16321 cggaactgcc gggcagatcc gcgtcaagcc gccttgtgtc tttacgtgtt atttccccag
    16381 ccagcagtgc agacaaagct gatccgccgg aaggccgatg gccgcgatca tgtcttcaat
    16441 cgtcatatac ctcagcgacg tcacgtttaa atcctgccgg atccagtcga tcattttcgc
    16501 gtgcttcgcc gacgtcgtgt cgagatacgc tgaaatatcc tcaaggtccc ttccctccat
    16561 cgcgcggatc gctttccggc agaccagttc ggaggttgtc cgggtggagg aggcataaat
    16621 gcacggatac atcaacggcg gacaggccgg gcgaatgtga atttccttcg cgccgttgtc
    16681 ccataatttt ttgacggtca aattcttcag ctgcgtgccg cggacgatgg aatcgtcgca
    16741 gatcaccatc cggctgccga aaatgacatc ccgcacggca accagcttca tggtggccac
    16801 cagatcgcga atttcctggg tcgggggtgt gtagcttctt ccatagccgg gcgtgtattt
    16861 caccaggggg cgccggtagg gaatgccgga ttccatggcg tagccgacgg catgcccgac
    16921 gccggaatcc gggacgcctg cgacgaaatc cactttcaca tggtcgcgcc tggccagata
    16981 acggccgcac cgctcccggg tgctttccac gccgacgccg tcgtagatgg aggaaggaga
    17041 accggtataa atccacagaa aggaacatat tttcttctgg ctcccttccg gtttgagctg
    17101 ccggacgccg tcgggggtca aaagcgcaat ttccccgggg cccagaaacc gctgcagttc
    17161 atatcccagg ttctcaaagg agctggcttc ggtggcgacc acataggatt tccggttggg
    17221 atccttgctc cggcccagcg caagcggaaa gcgcccgacc ttgtcccggg ccgcgtaaat
    17281 gccttccgcc gtcagcacca gaacacagat ggacccttca ataatcccgc gcatggacgc
    17341 gatgccctcg acaatgttgt cgccccggtt gatcagggaa gcgacgattt ccgaattgtt
    17401 gacgcctccg cccgccactt ccgtaaagga cgcgccttcg ttgagcagct cgccgaccag
    17461 ctcgctttta ttggtgatga gccccgtagc aaccaccgcg taaacgccga atttggaacg
    17521 gaaaataagc ggctgcggcg attcgtcgtc gatggcgccg atgccggagt gcccttccat
    17581 cttccggtag tcatcgacaa accgcgactt gaactgcgcc tgcgatattt cgtgaatcgt
    17641 gttgtaaaac cccctgggcc cgtaaacggc catgccgccg tgctcggttc ccagatgaga
    17701 atgataatcc gttccgtaaa acagcgtttg aacacaactg tcctcggaaa ctacgccgaa
    17761 aatccctccc atgattccct tccttcagaa gattgtttaa cccagcttcg cgacggcgtc
    17821 tttgattctc cggacgcctt cttcaatgtt cttcaatgag gttgcgtaag acaggcggat
    17881 atgatcgtcg ctgccgaatg ccacccccgg cacagtggcc acgttggcgc cgtcgagcag
    17941 atattcgata aactccgttg aattcgaaat tttcttcccc tcgtacgaac ggccgtagag
    18001 agccgacaca ttggggaata catagaaagc gccctcgggc atcggacaga aaatccccgg
    18061 aatatcgttg agcgcatcga caatatagtc cttcctcttt ttgaattccg cgaccatcat
    18121 ggagacgacc tgctgatcgc cggcaagcgc ggcaaccgac gccttttgcg atatggaggt
    18181 cgggttggag gtgttctggc tctggatctt gctgatcgcg ccgatgattt cttccggacc
    18241 cgcggcatag ccgatccgcc agccggtcat ggcgtacgcc ttggaaacgc cgttgaccac
    18301 aatggttctg tccttcaggc ggggttccgc catggcgaga ttggcaaagg ggaagtcggc
    18361 atagaggatc ttttcgtaga tgtcgtccga aatggcaaaa atatccgtat ccgccaaaac
    18421 ggcggccagc gccttcagtt cgtccggcga ataggccgcg cccgtcggat tggacggacc
    18481 gttaaaaatg acggcccggg tgcggccggt gatcgacgct ttcagctgtt cgggcttcat
    18541 cttgaagccg tctttctcca gcgtgttgag gatgaccggc gttcctccgg ccagcacgac
    18601 gatatccgga taggaaaccc agtagggcgc cggaatgatg acttcatccc cctcttcgaa
    18661 gagcgcctgc gccagattgt acagcgtgtg cttggcgccg caggaaacga ccacctgcga
    18721 ccgcttgtac tccaggcggt tgtcggtctt cagtttggca atgatcgcgt ctttgagttc
    18781 atccgttccg cccaccggcg tgtattttgt aaagcctgcc tcgatggccc ggatggccgc
    18841 ctgtttgatg tggtcggggg tgtcaaaatc cggttcgcct gcgccaaaac cgatgacatc
    18901 ccttccctgc gcccgcaggg cattggcttt ggccgtaatg gccagggtct ccgacggttt
    18961 cacttttgct actcttgacg ccagtttcac aaacgacctc ctgttgtttt tttattggga
    19021 tatttttcga gcagtctttg atacacattg tccggaacca gcccccggac cgtccctccg
    19081 aggctcgcca cttccttgat gatattggag ctggtgaaaa accatcggaa tccgctcatg
    19141 agaaaaacgg tctcgacgtt tttggtctgg cggcggttca tcagcgccat ctggaattca
    19201 tattcaaaat ctgaaagggc gcgcatgccc cgcagaatcg tgctggcgcc ggatttctgc
    19261 aggtaatcaa tcagcaggcc gtcatggctg tccacctgaa tgcattcaca acccttgaat
    19321 atttcgcgga tgagctggca acgctcttcc atactgaaaa gaaatgattt tcgtggatta
    19381 taggcaacca gaacgatgat tttgtcaaag atccggctgc cccttttgat gatatccaca
    19441 tgaccgttgg taatcggatc aaacgagccg ggataaattg cgattttgta gccggttttt
    19501 tcttgtgtca tgcgccgtcc atccttataa acgttaatag attttcccca tattttctct
    19561 ggtccgccac caaccagggt tttggaagtt cgggcagcgg ttcccggacg gaatgctgga
    19621 gcacaaggat tccaccctcc cggatgaccg gacagttccg cagggccccc agcgtctctc
    19681 caatgaagcc ctgaccgtag ggaggatcgg caaagataac atcaaacctg cccggttgat
    19741 cgtagaggac gcaaagcgcc gaagcgacgc cgtcatgaat aatccgggct tgctccgaac
    19801 aaccgcataa agaaacattt tcgcgaagaa cgccgatcag ttttccgctt ttttcaacaa
    19861 aaacaatttg tttcgccccg cggctcgccg cttccaatcc cacggatccc gagccggcaa
    19921 acaagtccag aaaactccgg tccgtttgcg gtcccagtaa attaaaaaga gcttcccgca
    19981 aaaaatccgt cgtcggtctc gacgatgacc gggagggaaa atgcaatgtt cttcctttaa
    20041 acttcccgcc ggtgattctc ataacctcat ggccggaccg ccccgatcat cccttcactt
    20101 ctccccgccg ggaagatctt tggcgtccgt ttcggtgccg tgcttcttgt tgatgacttt
    20161 gcaaagccac cagacggggc cggacaaagc gtagccgacg gtgatgacaa aaatcgtcac
    20221 ttccggttta taaataatca ccagcaagat gccgaccacc accagaaaag tcatgaacgg
    20281 catgcgggca aagagcttca tgtcttttcc gctgtagtat ttgatgttgc tgaccatcaa
    20341 aaaagccaga atgacgacgc agatcatcac gtatgggttg tggaaaatgc cttcaatgcc
    20401 catgacatca ttgacgaata taaccgttgt ggcaatgacg gccgcagccg cgggaatggg
    20461 cagcccgtta aaaaccttgc tgtcgatcag gccgatctga atattgtacc gcgccagacg
    20521 taaagcgccg caggtcacaa acagaaagcc ggccatccag cccagtttcc cgtaggaaga
    20581 catcgcccac atgaaggcca gcagggacgg agcgacgccg aaagcgatca gatccgccag
    20641 agaatcgtat tccgcgccga atttgctggt ggtgttggtg atgcgggcga tccggccgtc
    20701 caggccgtcg aagaccatgg acaccagaat ggcaatcgcc gccacaaaga acttctcctg
    20761 aatcgaggcg atgatcgaat aaaaccccat gaacaagctg gcggtggtaa tcaggttcgg
    20821 cagaacataa atgcccttct tgagccgggt attgcgaatg tggcgccctg aattcatgaa
    20881 aggtatccta gtggtgtttg tcccgcccgg accttatcgt tcaggcggac aacaacgcgg
    20941 gaatcggcag gcagaaaaac ctcaacacgg gaaccgaaac ggatcatgcc gaaccgttcg
    21001 cctttggcaa gtgtttcccc ttcacggatc cagcacacaa ttcttctggc gataagcccg
    21061 gcaatctgca cggtccaaat tgcgcggccg tcttccgtgc ggatcagcac ttcgttgcgt
    21121 tcattctccg tcgaggcttt gtccagattg gccgacacaa atttccccgg gtgataggat
    21181 atcttctcta tcgttccttc gtagggagcg cggttgacgt gaacattgaa cacattcata
    21241 aagatgctga tttttttgaa ccggccggcc attgcgcccc ggacatctac ctcttccatt
    21301 ttaatgactt tgccgtcggc cggactgatg accagctttt ccgcagcctg aaagtgcctc
    21361 tgggggttgc ggaaaaaagc aatgataaat atcgtcaatg cggcgagcag aatcgtcagc
    21421 caggccacgc cgaaaaaagc tgcaaaacca gtgaagacaa gggagaggac gatgaaagga
    21481 aaaccttcag ctacgataat actcttatgt ttcataccgt atattatctt tcctcgattg
    21541 aggttttgtt gatatatgaa ttgaggggtc ttgtcaataa atctttgtcc tccccgattc
    21601 gaagatcatc ttgacagaac ctcccgaaat cctatataaa accaaaaaat cggag
//
LOCUS       DAOSRM010000028        21148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2684_length_21148_cov_103.804532, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000028 DAOSRM010000000
VERSION     DAOSRM010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 21148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21148
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2684_length_21148_cov_103.804532"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1583)
                     /gene="nhaA"
                     /locus_tag="PLA58_04380"
     CDS             complement(231..1583)
                     /gene="nhaA"
                     /locus_tag="PLA58_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOR62064.1"
                     /translation="MAKHQYPLEPLFGKIISPFERFLQQVTAGGIILMGMTLLTLIIA
                     SSPWGDAYRHLWETPVRVGIGRENMEMSLHAWVNEGLMALFFLLVGLELKREILVGEL
                     SSLRNTALPVAGAIGGMLAPAIIYYLFNPSGDALAGWGIPMATDIAFAVGILVLLAWR
                     IPKNLIIFLTALAIADDLGAVLVIAIFYTKNIDMMSLGAAIGLLGILVLCNRGGIRYP
                     LPYAILGILLWMALLKSGIHATLSGVLLAFTIPAHPFYTPREFDEHLNKIKNDFRGEI
                     LTSEEVDTELDVTRTYAIAESIEKASSAVQSPQQRMEHHLSPWVTFGVIPLFAMANAG
                     LDLLAMPFRDVIVQPVTLGVMLGLIFGKFVGISGFAWLAVRLGLARLPDGVQWRHILG
                     AAWLCGIGFTMSLFIAQLAFTTSPRLFEEARLGIIMSSVIAAIVGLTWLYLSARKVPS
                     "
     gene            complement(1594..1896)
                     /locus_tag="PLA58_04385"
     CDS             complement(1594..1896)
                     /locus_tag="PLA58_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62065.1"
                     /translation="MKKVKISFVVLMGLILMLSACTRLNPPVEISGKSASYIECGKNP
                     PTPGDMARHTLKGEKTFEWRCFDGHLETGETFVGVFHARSFTSGKPVVTFSLYQIS"
     gene            complement(2053..2847)
                     /locus_tag="PLA58_04390"
     CDS             complement(2053..2847)
                     /locus_tag="PLA58_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOR62066.1"
                     /translation="MFRIKKIPTSLILLLVLADVAFAAHPLITDDANTQGRGNVQVEV
                     NGQISINKYKVSDDDGEPLTVRSRESELKTTVSYGIVDSVDAMVTIPYQWETIETGSS
                     AFFHEDGVADVALEIKWLFLKKNGFLFALKPGITLPVGDTDKNLGSGRMNGSLFLITT
                     REMTYGNLHLSLGYIRNENSIDQKEDIWHASLAGEWKVVKALRLVANAGMEENPDKLS
                     SVDRAFVLGGVIFSPAANLDIDAGIKAGLTDTETDWVFMGGVTFRF"
     gene            complement(2866..4620)
                     /locus_tag="PLA58_04395"
     CDS             complement(2866..4620)
                     /locus_tag="PLA58_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOR62067.1"
                     /translation="MAAPQNPETNPFARQNHRGGLARWIPLLATLRGYQRGWLWRDLA
                     AGLALTALLVPVGMSYAAASGLPAIYGLYATIVPLIAYAVFGPSRIMVLGPDSALVGL
                     IAATILPLSAGNPEKAVALASMLAVLSGLLCILAGLAKFGFITDLLSRPVRDGYINGI
                     ALTVLVGQLPKVLGFSVSPGSFLAETGDLLQGIGSGQIHAMACVIGLSCLAVILGFRR
                     WAPMVPGVLIAVAGATIFVSWFPAPIREGIAVIGPLPQGLPQFRIPDVSPAEIGELFA
                     AAVAIALVSFTDMSAVSRAFAHRTGGEVDSNQEILALGAANVAAGLFSGFPVSSSMSR
                     TPVAEAAGAKTQVTGLVGALCVALLLLFAPGLLFHLPQAALGAVVVAACLSLIEIRSV
                     LRLYRLRRDEFVLSLACFLGVVLLGVMPGIFIAVSLSLLAFIWRAWQPYSAVMGYVEA
                     LGSYHDISRHPEAKCIDGLVLFRWDAPLFFANAELFRRRVLRAVSHAPTPVRWVVVAA
                     EPITDVDMTAAGVLDELDAALHAAGMDLCFAEMKGPVKDRLKRYGLFNRLGVENFFPT
                     IEQAVEHYLSLNRMDGPA"
     gene            4920..6239
                     /locus_tag="PLA58_04400"
     CDS             4920..6239
                     /locus_tag="PLA58_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62068.1"
                     /translation="MIRSHCSTPFKTLAGLLAVFVFLSLSGCDYLKFVGQKRTLKNEF
                     HNHPKMELLRELSPENCYTLFGELDHPKDYDGSFLVVGVSDRYQNREIVAKRVVLNPA
                     LFYSVMAPEGDYDIYVFADLNGDDLFRDNELVGRTTPGKPIPIGRKYAEDNILVKGPT
                     LRLDLDHPQKADFPLAVGGITRKSRYASLDDEFFDPRYGTMGLYRPMELLAHTQGPFF
                     QLEDYDPHKTLILFVHGVEGTPQDWKYIVEGLDRKKFQPWFYFYPSGLPLEKLGNTLA
                     RSIMLLDQTPGYGISNLIIVAHSMGGLVARAAIDRLCRDGKPRYFRMYISFSSPYGGI
                     EEAREGVKSAPAMVPCWIDVASDSEFLQRIYSTDLPKDVPFYLFFGYRNKSGASGDGT
                     ITLRSQLDDRIYVKAVKTYGFDATHVGILNDASVRKVFNEILETVEP"
     gene            6273..7412
                     /locus_tag="PLA58_04405"
     CDS             6273..7412
                     /locus_tag="PLA58_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOR62069.1"
                     /translation="MKKIFLLLSILMLVHPAWADETVKIGFLGPTTGPFASVGREAGQ
                     VLTLMAADINGRGGLLGRKVEMVFASEGEDDRTAASAAQQLIQQGVVAVIGPHTSDRT
                     QAVQSIFHEAGILQISYGATAVPLTEKGFPYFFRTCPRDDEQAKAFVRVLRKLNFKKI
                     AVLHDQSLYGKSLAQAVAHQLYAWMISTVYSGSLTPGQSDDTELLKKIQAAAPDMIFF
                     AGYYPDAAGLLRAKRQLQWKVPLMGGDGVNHPQLAALAGKDAAEGFHFLSPPNPEHLE
                     TPPAKAFLERFRKVHAGNPSSVYPLLAGNAFLAFTQSATQMKTTETKAVADYLHRRYF
                     NKSGLTGEIFFNVWGDVVNDLYAVYFVDANGRFILKRQILHGDFI"
     gene            7465..7866
                     /locus_tag="PLA58_04410"
     CDS             7465..7866
                     /locus_tag="PLA58_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HOR62070.1"
                     /translation="MKKYIVLSMILLLAGCTVSPVLYPNAHLSSVGNDQAERDIALCR
                     EQADLYIKTNPAGETAKGAAIGGIGGAVVGGAMGAVDGSFGKGVGMGAAGGAAAGLVG
                     GILRASQPDPLYINFVNRCLNEKGYEIVGWQ"
     gene            7887..8303
                     /locus_tag="PLA58_04415"
     CDS             7887..8303
                     /locus_tag="PLA58_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62071.1"
                     /translation="MKISLLDRSLYYKGLMILIRQDREIHEKEKKLMMSIGKKLGFER
                     KFCAKTIEHILVNHYITDEPPRFSKTGVARSFLLDGLRLSSIDGRIHEAEIAWLASVA
                     ESNGLADFWAAALEQFRSSPPAGISDDHFEVTKFQW"
     gene            complement(8328..9161)
                     /locus_tag="PLA58_04420"
     CDS             complement(8328..9161)
                     /locus_tag="PLA58_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOR62072.1"
                     /translation="MAEKNLVYRGKSKDVFRITSGAHKGKYRFVFTDRGTGYFENGKP
                     VFDPGYDVVVGEIPGKGAIACRFATYFFRLLKSKGVATHYIDTISDNEMIVEPAVPLG
                     MKAQGPAFPGSAPLSNLEFTWRNNATGSFWRRYPFVRPCKNLHRVVEAWTKGDSDILI
                     TMDALAETGALTRKEILQSVKLVQKIADIVSKEFASRNLHVIDGKFELGRLKYGDGKI
                     VLIDEISPDVLRVCRGFRPDGDGDCTKFKTCTVTGESDGKRTIKNKNQVSAAELVKIF
                     R"
     gene            complement(9493..9924)
                     /gene="tnpA"
                     /locus_tag="PLA58_04425"
     CDS             complement(9493..9924)
                     /gene="tnpA"
                     /locus_tag="PLA58_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOR62073.1"
                     /translation="MGDGCLPHTKWECKYHVVWMPKYRKKTLYVELRKYLGSTFRDLA
                     AQKECEVMEGHLLPDHIHTLISMPPRYSVSQVVGYLKGKSAVQIARTYMGRKKNFTGQ
                     HFWARGYFVSTVGADEKTVRDYIRRQETEDYRLVQLGMFEE"
     gene            complement(10135..10211)
                     /locus_tag="PLA58_04430"
     tRNA            complement(10135..10211)
                     /locus_tag="PLA58_04430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10175..10177),aa:Arg,seq:cct)
     gene            10333..10578
                     /locus_tag="PLA58_04435"
     CDS             10333..10578
                     /locus_tag="PLA58_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62074.1"
                     /translation="MMDKTFTGTPAIHRIIKIADPWIEVSHRVAKKCGKSQANVQLKE
                     CISFGMLTVFSAIKKPPVQRGCGEIFEMAEVGQEKPG"
     gene            10843..12711
                     /locus_tag="PLA58_04440"
     CDS             10843..12711
                     /locus_tag="PLA58_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOR62075.1"
                     /translation="MAGDNLESSLQWIKENFSNGQVNFATLEDVQQWACCHEQNIDRY
                     PSAWCAVEMAMLDLLAREKNCSVEKLLRLDDFRGYGRYTAVIGDDKGWKYAALADQYL
                     IRGLRDFKIKLSGNLERDLEKMDLLETLSMQHQVPSPRIRLDANNLWKNHCDEAVAYT
                     QALGIGRIFAMEEPVEAGHVEEISKFSVATGLPVILDESLCTLKDLDRFKTAPGQFIA
                     NIKISKLGGLIRALRIIDEVKKLGWPIIIGCHVGETSLLTRAAFAASSAAGENLTAQE
                     GAFGDYLVKREPVEPMLKFGRNGILDLRSPYYLKTVQGLKVIPAENWNVGFGMQGHLP
                     HSPDDGAPDICFLEMPDQYKIHYRRWGHSEGENVALLLHGDMGHSGWLAPLASRLRSL
                     SPDLTVIAADRRGCGLNEQRGDLGSVQAVIEDVIQHIKFLKRSFKQVHLAGWGQGAQY
                     AAVAGTRMGNAVSSLILLTPGFFWNEQFQSVSRITRKIVPEILAEFKLKPERNHACIP
                     IPMEATDFTLENEWLDFIERDPLKTTMVTLKSVSIMDEIQELSWGAIPQNHLPTLVMM
                     GQQDRIVDNRKVLEFIGHLFQGKNRNRLVTVESGHAIHFEKTEEVASEIVNFIRKL"
     gene            complement(12716..>12947)
                     /locus_tag="PLA58_04445"
     CDS             complement(12716..>12947)
                     /locus_tag="PLA58_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62076.1"
                     /translation="GMMEVHIYHILHKMKAIPQVDEFALPDGLFRIRVPDQIFMMETQ
                     WNRSKDMLGGVLALHDCFLTVWVHSAAPLLLI"
     assembly_gap    12948..13047
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13048..13380)
                     /locus_tag="PLA58_04450"
     CDS             complement(<13048..13380)
                     /locus_tag="PLA58_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62077.1"
                     /translation="MYNLGFDALATIAPGPPLTQDVPAVGRLDGKKFFLDVLELGRDK
                     KQRKSIGEDDVQYRRIFGKSAAPLRPAAGHHTVVAWFRIGPEKKTLMIVGMMEVHIYH
                     ILHKMKAIP"
     gene            complement(13489..14001)
                     /locus_tag="PLA58_04455"
     CDS             complement(13489..14001)
                     /locus_tag="PLA58_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62078.1"
                     /translation="MIHIDFVGDMSAGIDNIHDHMTQPVIVDDIHVQDDKTIADFFLF
                     LHPSIENLRLAFDLQSAGIIHGQVRNDHSHRKDILIRRNINGEARNREIMNIQLNSLV
                     DGRMVKGNIRKRLDLNTRIAPFFFRKTVHPVYHRGKDDIPCRLIRCLPDAAASCRKDH
                     LFIRIPIDRR"
     gene            14077..15012
                     /locus_tag="PLA58_04460"
     CDS             14077..15012
                     /locus_tag="PLA58_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62079.1"
                     /translation="MKETYRRLYRDNPEIATQITYQQNGRIYGHASMVRSYRRTWMVH
                     HLAARPLNNKRTGLQVLKQIMHYFNGLYRLPSVGMDYMMFYFRPDNQFPDHFFGGFAR
                     HFRNPRACSLDLFAYLNYPVSGVGRQLPAGWLLEPCTGEDWEALSHFYRNASDGLLLD
                     VLRLGEEKEDGESLSQLYARHGFIRSCQCFSLKQNGRLKAVLIVNRSDPGLSLSDFLN
                     GIKILITDAAGLPWEVLSTAVSQWSDFYKSDKIPLLVYPSRYFEEKGVSFEKYYNLWI
                     IDGHYAREYSEYMMENSKLRIRFLIRFLIKRYLKP"
     gene            15009..>17317
                     /locus_tag="PLA58_04465"
     CDS             15009..>17317
                     /locus_tag="PLA58_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR62080.1"
                     /translation="MMDNHTHIYNSRIIKSYVEYLKKHLPEVDSDPLLKYADIETYQL
                     DDEGHWLTQEQVDRFHDILAKTTQDPDISRKVGRFSVTSRASGALGQYMLGFINPATA
                     YGVLERINAQLSRGATLKTKAIGTDRIEAKATLQAGVVEKPYQCQNRLGGLEALAKLS
                     TNKFASIEHPVCIHKGGDCCLYVISWENTRTFFWKRIRNYYFWIAFALCAILFSFMKP
                     AYWDVLTLSCVLMGMGVTLYAEHLEKKDLLAHIFNQGDAADSLLDQINRRYNEALLVQ
                     EIGQKSSDMTKVDELLQYIIESLVKRLDFDRGIIMLANKQKDRLVYTVSYGYNPKNED
                     YLHNIQFHLDNPQSKGTAVASFRTQKPFLVNDLSDIEHDLSRKSLDFVNTMGSRSFIC
                     VPIVYKGESLGVLIVDNVQSKKPLSQTEMSLLMGIAPQIGISIKNAIAHQKIKESEEC
                     FRSLSENAPDIIYTLGIKGKFTYINPVIETILGYRPEEIIGRYFIDIVRKEDVHRCIE
                     FFKQVRDHQQTIKEEMGILLHKDGTERYFSFSCAPNFDSESHFIGVVGTFKNMTDIRK
                     SEMELKKSFEKLQSVMSSTIDAISIIVESRDPYTAGHQRRVTQLATAIACELALSKEK
                     IDLIRMGSLIHDIGKIYIPSEILTKPMKLNDIEYAMIKSHPEVARKILKQVDFIPTVV
                     DMVYQHHERIDGSGYPRKLKRDDILIEARILAVADTVEAMATHRPYRPSLGIEKALEE
                     IENQRDILYDEAVADACLVLFREKGFQFK"
     assembly_gap    17318..17417
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17525..18430)
                     /gene="ftcD"
                     /locus_tag="PLA58_04470"
     CDS             complement(17525..18430)
                     /gene="ftcD"
                     /locus_tag="PLA58_04470"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOR62081.1"
                     /translation="MKIIECVPNFSEGCDEAKIDSIARVLKASGGIRLMDYGGDRDHN
                     RSVFSFLGRPEDVLAAALAAAGQALKVIDMRRQTGVHPRLGAVDVVPFIPLGKTKMED
                     AVNLARLFGNRLYELFGVPVYFYGEAALVPGRRELADVRRGGYEALKEKMSIRGDAPD
                     VGEPVFHPGAGATAVGAREPLVAYNINLNSDDLRLAQKIASLIREKSGGLPHVRAIGV
                     ILKSRHLAQVSMNLTSCRETPLKAVYERVEALAAERGVQILESELIGLAPKCAFAGTR
                     PEKLKLVNFSKDRLLETHLKEFASA"
     gene            complement(18427..19074)
                     /locus_tag="PLA58_04475"
     CDS             complement(18427..19074)
                     /locus_tag="PLA58_04475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOR62082.1"
                     /translation="MNLFAIGDIHGCLDPLKRLLRKIPADPAGDTLVFIGDYIDRGRE
                     SKGVVDYVLGLRGACAKLVCLCGNHESMLLNYLEGVEEDLYLQNGGAATLRDYGIFLS
                     DSPRVRKGKIPAEHLSFFESLLPYYETDQYLFVHAGLRPGAPVACQSAWDLQWIRREF
                     IDSEDDFGKRVVFGHTHFNAPLVAENKIGIDTGAVYGGSLTCVQLPALKFYQVST"
     gene            complement(19101..20012)
                     /gene="ftsY"
                     /locus_tag="PLA58_04480"
     CDS             complement(19101..20012)
                     /gene="ftsY"
                     /locus_tag="PLA58_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOR62083.1"
                     /translation="MGLFDKLKAGLAKTRNALTKNLDQLVFGKRELNPALYDELEELM
                     ISADMGPQFTFDLIEDVKQRVKRRELSDASQIKKVLQERMIAILRKMEKPLAIPKNEA
                     FVIMAVGVNGSGKTTTIGKLAHMLRRDGHEVVLVAADTFRAAAIEQLEVWGARVGAPV
                     IKQKMNADPAAVVFDAAGKIKAGFNGVVIVDTAGRLHTRTNLMEELKKVKRVIGRELP
                     GAPHEILLVLDATTGQNAVIQAKMFQEEIGATGIVLTKLDGTSKGGVVVRIAGELGLP
                     VRFIGVGEGLEDLRPFDSGDFTAALLD"
     gene            complement(20429..20863)
                     /locus_tag="PLA58_04485"
     CDS             complement(20429..20863)
                     /locus_tag="PLA58_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOR62084.1"
                     /translation="MLKATGFKSQSLITVEADEFVSAAIQKLIQHDKGALPVTDEKNE
                     LIGIITERDIVRKCLARGGNCDDKKVGDIMTHEVAIASMDDDLDYAINTMRHKKIRHI
                     PIADGKRVVGMISMRDILGFQYEETKAEIKYIKLMPQRPVFK"
BASE COUNT         5037 a         5768 c         5416 g         4727 t
ORIGIN      
        1 gcccgcgaaa tcggttttct gcgctcatca aagccgctgt tcccggaaaa gcgacttcca
       61 tagaatgaaa accgggtcat ccggctgatg cgcacttctg gcaataattg gtttattgac
      121 cactcgtttc acccaccaaa gtgtgagcac tgcgaaatag atattattga agattacgac
      181 ttgcaagtta gcatcaatca tggatgttac tcctagtggt aacattttcg ctaactgggc
      241 acttttcggg cactaagata gagccaggta agccctacaa ttgcagcaat cacggatgac
      301 ataataattc cgagtctcgc ttcttcaaac agcctggggc tagtagtaaa agctaattgc
      361 gctataaaga gggacatcgt aaagccaatt ccacaaagcc aggctgcgcc taggatatga
      421 cgccattgga caccgtcggg gagtcttgcc aaaccaagtc ttacggccag ccaagcgaag
      481 ccggatattc cgacaaactt tccaaaaatc aaacccaaca tgacccccag tgttacaggt
      541 tgaacgataa catcccggaa aggcattgca agcagatcca gtccagcgtt ggccatagcg
      601 aatagcggga taaccccgaa tgtaacccag ggagaaagat gatgctccat ccgttgttgt
      661 ggtgactgaa cagcagatga tgccttttca atgctctctg caatggcata tgttcgggta
      721 acgtcaagtt ctgtatcaac ttcctcagac gtaaggatct cccctcggaa gtcatttttt
      781 attttattaa gatgctcgtc aaactcgcgg ggtgtataaa atgggtgtgc gggaatcgtg
      841 aaggccagca acacaccgga caaagtggcg tgtatccccg atttaaggag agccatccac
      901 aggagaatcc ccaggatagc atagggcaaa ggataacgga tacctccccg gttgcagaga
      961 accaggatac ccagcaggcc aatggcagct ccaagagaca tcatatcgat gttctttgta
     1021 taaaagattg ctatgactaa cacagccccc agatcgtcag caatggccag agccgtcaaa
     1081 aagatgatga ggtttttggg gatgcgccag gccagaagaa caaggatgcc cacggcaaag
     1141 gcaatatccg tggccatcgg aatcccccaa cctgccaaag catcaccgga tggattaaat
     1201 agataataaa tgatcgccgg agcaagcatg cctccaattg cccccgccac cggaagagcg
     1261 gtattacgca gagaggaaag ctctcccacc aatatttcac gttttagttc gaggccaacc
     1321 agcaaaaaaa acagggccat gagcccttca ttcacccagg cgtgaaggga catttccatg
     1381 ttctccctac cgatcccgac gcgaacgggc gtctcccaca aatgtcgata agcgtcaccc
     1441 catggcgaac tggctataat taatgtcagc agcgtcatgc ccatgagtat aatcccgccg
     1501 gccgtgactt gttggagaaa ccgctcaaag gggctaatga ttttcccgaa aaggggttcg
     1561 agaggatatt gatgctttgc catgtgtctc tccttaactg atctggtaga gggaaaaggt
     1621 tacgaccggt ttgcccgatg tgaaactccg ggcgtgaaag acgccgacaa aggtttctcc
     1681 cgtttcaaga tgcccgtcga agcaccgcca ttcaaaggtc ttttcccctt taagggtgtg
     1741 tcgcgccatg tcgccgggtg tcggcggatt tttaccgcat tcgatataag aagcgctttt
     1801 gcctgaaatt tccaccggcg gattcagacg cgtgcatgcg gaaagcataa ggattaatcc
     1861 cataagaacg acgaatgata ttttgacctt tttcacaaaa ttgctccttt tcgttggacc
     1921 tgttttgaac ggttcccgcc tgactcgctt ccggacggaa aatttctttt gacgacgggg
     1981 aaagtttagg aaacgcaccc tcggatgtca aggaggtctt gagaatcttg aaaaaacggg
     2041 gttgccgtcc ggtcagaacc ggaacgtgac gccccccatg aacacccagt ccgtttccgt
     2101 atcggtgagc cccgccttga tcccggcgtc aatgtccaga ttggcggcgg gcgagaaaat
     2161 gactcctccc agaacgaagg cgcggtccac gctggagagc ttgtccggat tttcctccat
     2221 gcccgcgttg gcgacaaggc gcagcgcctt cacgactttc cattcgccgg ccagggacgc
     2281 gtgccagata tcttccttct gatcgatgct gttttcattt ctgatatagc cgaggctcag
     2341 atgcaggttt ccgtaagtca tttctctggt ggtgatcaaa aacagactgc cgttcattct
     2401 gccggatccc agatttttgt ccgtgtcccc caccggcagc gtaatgcccg gcttgagcgc
     2461 aaagagaaag ccgttcttct tcaaaaacag ccattttatt tccagggcga catcggcgac
     2521 gccatcctca tgaaaaaagg cggatgaacc ggtttcaatg gtctcccact gatacggaat
     2581 cgtcaccatc gcgtcaacgg aatccacgat gccgtagctc accgtcgtct tcagctcgct
     2641 ttcccggctg cgaaccgtga ggggctcccc atcatcgtcg gataccttgt acttgttgat
     2701 gctgatctgg ccgttcacct ccacctgaac attccctctg ccctgcgtgt tggcgtcgtc
     2761 ggtaatcaaa ggatgagcgg caaaggcgac gtccgccaga accaaaagca ggatcaggct
     2821 cgtcggaatt tttttgatcc ggaacataga cctcttctta tccgcctaag ccggcccgtc
     2881 catcctgttc agggacaggt aatgctcgac ggcctgctcg atggtgggaa agaaattttc
     2941 gacgcccagc cggttgaaca ggccgtagcg cttcagccgg tctttgaccg gtcctttcat
     3001 ctccgcaaag cataaatcca tccccgccgc gtgcagggcg gcatcgagct cgtcgagcac
     3061 gccggccgcc gtcatgtcca catccgtgat cggctcagcc gccacaacaa cccagcggac
     3121 cggcgtcggc gcatggctca ccgctcgcag aacacgcctg cggaacaatt ccgcattggc
     3181 gaaaaacagc ggggcgtccc agcggaatag caccagaccg tcaatacatt tggcttccgg
     3241 gtgacgggaa atgtcgtggt agctgccgag cgcctccaca taccccatca cggcgctgta
     3301 aggctgccag gcgcgccaga tgaacgccag caacgagaga ctgacggcga taaaaatgcc
     3361 cggcatgacg cccagcagaa ccaccccgag gaaacaagcc agcgataaaa caaattcgtc
     3421 gcgccgcaga cggtagagcc gcagaacgct ccggatttct atcaggctca ggcaggcggc
     3481 caccacaacg gcgcccagcg ccgcctgagg cagatggaag agcagtccgg gggcaaaaag
     3541 cagcaggaga gcaacacaaa gcgctccaac caaccccgtg acttgcgtct tggcgcccgc
     3601 agcttctgcc accggcgtgc gcgacatgct gctgctgacg ggaaagcccg aaaaaaggcc
     3661 ggccgccaca ttggccgcgc ccagcgcgag gatttcctga ttgctgtcca cttcccctcc
     3721 ggtgcggtgc gcaaacgcgc gcgaaaccgc gctcatgtcg gtgaaggaca ccagagcgat
     3781 cgcaacggcg gcggcaaaca gctcgccgat ctccgcaggc gacacgtcgg gaatccggaa
     3841 ttgcggcaat ccctgcggca gcggaccgat gacggcgatt ccctcccgaa tgggcgctgg
     3901 aaaccaggaa acgaatatcg tcgctcccgc cacggcaatc aacacgccgg gaaccatcgg
     3961 cgcccaccgg cggaatccga gaatcaccgc cagacaggat aaaccgatga cacacgccat
     4021 cgcgtgaatt tgtccgcttc cgatgccctg cagcaggtcg ccggtttccg caaggaagct
     4081 gcccggactg acggagaagc cgagcacttt gggcaactgg ccgaccagca cggtcagcgc
     4141 gatgccgttg atatagccgt cccggacggg cctggagaga agatcggtaa tgaagccgaa
     4201 tttggccagg ccagccagaa tgcacaacag cccagacaaa accgccagca tggaagccag
     4261 cgccaccgcc ttttccggat tgccggcgga aagcggcaga atcgtcgcgg cgatgaggcc
     4321 gaccagcgcg gagtccggtc ccagaaccat aatgcggctg ggaccgaaaa cggcataggc
     4381 gatcagcgga acgatggtgg catacagacc gtaaatggcg ggcaggcccg atgccgcggc
     4441 atagctcatc cccaccggaa ccaggagggc tgtcagggcc agtccggcgg caaggtcgcg
     4501 ccacagccaa ccgcgctgat acccgcgcag cgtcgcaagc aggggaatcc agcgggcaag
     4561 accgccacgg tgattttgac gggcaaaggg attcgtttcc ggattttgtg gagccgccac
     4621 ggcgcacctc acggcattta gtgagagctt tgatcatctc tgtgtcaaaa gcgaacgaag
     4681 tgaagcaatc tcatcagcat tggatgtttg aagagatcga cacgtcactt cgctcctcgc
     4741 gacgacaaaa tcgtagtagt tatgtaaagc tctcttcgtg gctgtgagtt tataaatttt
     4801 tgactttttt tacaatatga aatcccttcc gcctgatttc ctccgcggca tttgtcattt
     4861 ttgtaaaatc ggtgtataga tcaattgctt tttccgaagt tccggcccgg aggtccgcca
     4921 tgatcaggtc ccattgcagc acgccgttta aaacgctggc aggactgctc gctgttttcg
     4981 tcttcctgtc gctatccggc tgcgactatc tcaaatttgt cgggcagaag aggacgctga
     5041 aaaatgaatt ccacaatcac ccgaagatgg aacttctgcg ggagctgtct ccggaaaact
     5101 gctacacgct cttcggcgag ctcgatcacc caaaagatta cgacggatca ttcctcgtcg
     5161 tcggcgtctc cgaccggtac cagaatcgcg aaatcgtcgc caaacgggtt gtcttaaatc
     5221 cggctctgtt ttattccgtc atggcgccgg aaggagacta cgatatttat gtgtttgccg
     5281 atctcaacgg ggatgatctt tttcgcgaca acgaactggt cggccggacg acgcccggca
     5341 agcccattcc catcgggcgg aagtatgccg aggacaatat cctggtcaaa gggccgaccc
     5401 tccgcctgga tctcgatcat ccccaaaaag ccgatttccc gctcgcggtc ggcggcatca
     5461 cgcgaaaaag caggtacgcg tccctcgatg acgaattctt tgatccccgc tacggcacca
     5521 tggggctcta tcggccgatg gagcttctcg cccacaccca gggccccttc tttcaactgg
     5581 aggactatga tccgcacaaa accctgatcc ttttcgtcca tggcgtcgag ggcacccctc
     5641 aggactggaa gtatattgtc gaggggctgg accggaaaaa atttcagccc tggttttact
     5701 tttatccgtc cgggttgccc ctggaaaagc tcggcaacac cctggcccgc tccattatgc
     5761 tgctggatca gacacccggc tacggcatct ccaacctgat catcgtggcg cacagcatgg
     5821 gaggtctggt ggcgcgcgcg gccatcgacc ggctgtgccg ggacggaaaa ccccgctact
     5881 tcaggatgta tatttccttt tcgtcgcctt acggcggcat cgaagaggca cgggaaggcg
     5941 tgaaaagcgc gccggccatg gttccctgct ggatcgacgt ggcgtcggac agcgaattcc
     6001 tgcagcgcat ttacagcacc gatctgccga aggacgttcc cttctacctg tttttcggct
     6061 accgcaataa gtcgggcgcc agcggcgacg gcaccatcac cctgcgcagc cagctggacg
     6121 atcggattta cgtgaaggcc gtcaaaacct acggctttga cgccacgcac gtgggcatcc
     6181 tcaacgacgc gtccgtcaga aaggttttca acgagatttt agaaaccgtt gaaccctgat
     6241 cacggcgcgg ttcgacggaa ggagaaaaac gcttgaaaaa aatattcttg ctgctgtcca
     6301 tacttatgct ggtccatccg gcctgggcgg acgagacggt taaaatcggt tttctcggac
     6361 ccacgacggg gcccttcgcc agcgtgggca gagaggccgg acaggttctg accctgatgg
     6421 cggcggacat caacggccgc ggcggcctgc tgggccggaa ggtggagatg gtctttgcgt
     6481 ccgagggaga agacgaccgg acggccgcct cagccgcgca acagctgatt caacaggggg
     6541 tggtcgccgt catcggcccg cacacatcgg acagaacaca ggccgttcag agcatttttc
     6601 acgaagccgg gattctccag atttcttacg gcgccacggc cgtgccgctc acggaaaaag
     6661 gattccccta tttctttcga acctgccccc gggacgatga acaggccaaa gcgtttgtcc
     6721 gggtgctccg gaagctgaac tttaaaaaga tcgctgtgct gcacgatcaa agcctttacg
     6781 gaaaaagtct ggcccaagcc gtcgcgcatc agctttatgc ctggatgatt tccacggtct
     6841 atagcggatc cctgacgccg ggccaatccg atgacacgga acttctgaaa aagattcagg
     6901 cggcagcccc ggacatgatc ttcttcgcgg gctattatcc ggacgcggcc gggctcctcc
     6961 gggcaaagcg acaacttcag tggaaggtgc ctctcatggg aggcgacggc gtgaatcatc
     7021 cgcaactggc ggcgctggcg ggtaaagacg cggcggaagg ttttcatttt ttgagtccgc
     7081 ccaaccccga gcatctggaa acgcctccag ccaaagcgtt tctcgaacgt ttccggaagg
     7141 tccatgccgg gaatccctcc tccgtgtatc cgcttctggc cggcaacgcc tttctggcgt
     7201 ttacccagag cgcgacgcag atgaaaacca ctgaaaccaa agccgtggcc gattatctgc
     7261 atcgccggta tttcaataaa tccggcctga ccggagagat ctttttcaac gtctgggggg
     7321 atgtggtcaa cgacctctac gccgtctatt tcgtggatgc caacggccgc ttcatcctga
     7381 aaagacaaat tctgcatggc gattttatct agcgatcgcc attttatgcg tcatcctgaa
     7441 agaaagggag gctggttgtt ccgaatgaaa aaatacattg tcctatccat gattctattg
     7501 ctcgccggct gcacggtaag ccccgtgctt tatcccaacg cccacttaag cagtgtcgga
     7561 aatgatcagg ccgagcggga catcgccctg tgccgggagc aggcggacct gtacatcaag
     7621 acgaacccgg ccggggaaac cgcgaaggga gcggccatcg gcggcatcgg cggcgccgtg
     7681 gtcggcggcg cgatgggcgc ggtggacgga agcttcggca agggcgtggg catgggcgcg
     7741 gccgggggcg cggcggcggg gctggtcggc ggaatcctcc gggcttccca gcccgatccg
     7801 ctctacatca atttcgtcaa tcgctgtctg aatgaaaagg gctacgaaat tgtcggatgg
     7861 cagtgaccga tcaaggataa gtcgtcatga aaatatccct gctggaccgg agcctctact
     7921 acaaagggct gatgattctg attcgccagg accgtgagat tcatgaaaaa gaaaagaaac
     7981 tcatgatgag catcgggaag aagctcgggt ttgagcggaa attctgcgcc aaaactatcg
     8041 agcacatcct ggtcaaccac tacatcaccg acgagccgcc ccgtttctcg aaaaccggcg
     8101 tcgcccgctc ttttctcctg gacgggcttc ggctctcctc gatcgacggc cggattcacg
     8161 aagcggagat cgcctggctt gcatcggtgg ccgaaagcaa cggccttgcc gatttctggg
     8221 ccgcggcgct ggagcaattc cggtcttcgc cccccgccgg catttccgac gatcacttcg
     8281 aagtgaccaa atttcaatgg tgacgcttcg cgcggcggcg ataacgccta ccggaaaatt
     8341 ttgacaagct ccgccgcgga cacctgattt ttgtttttga tcgtccgctt gccgtccgat
     8401 tcccccgtga ccgtgcaggt tttgaacttc gtgcagtccc cgtccccatc cggccggaac
     8461 ccccggcaaa cccgcaggac atcgggcgat atttcatcga tcagcacgat tttgccgtcg
     8521 ccgtatttca gccgtcccag ctcgaacttt ccgtcgatga catgcagatt tcgggaagca
     8581 aactctttcg aaacgatgtc ggcgattttt tgcaccagtt tcacgctctg gaggatttcc
     8641 tttcgggtca gcgcgcctgt ctcggccagc gcgtccatcg tgatcagaat gtcgctgtcc
     8701 cctttcgtcc acgcctcgac cacccgatgg agattcttgc agggccggac gaagggataa
     8761 cgcctccaga agcttccggt tgcgttgttt ctccaggtaa attccagatt ggaaagaggg
     8821 gccgaaccgg gaaacgccgg cccctgcgcc ttcatgccga gcggaacagc cggctccacg
     8881 atcatttcat tgtcgctgat cgtatcgatg tagtgcgtgg caacaccctt agatttcaga
     8941 agccggaaaa aatacgtggc aaaccggcag gcgatggcgc ctttccccgg aatttccccg
     9001 acaacgacgt cgtatcccgg gtcaaaaacc ggtttgccgt tttcgaagta gccggtgccc
     9061 cggtcggtaa aaacaaaccg gtactttcct ttgtgcgccc cgcttgtgat tcggaataca
     9121 tctttcgact tgccgcgata aaccaggttt ttctccgcca tctgtgtccc cccgtcagga
     9181 atgaattatc aaaataacag ccgttgccgt gccgttctgt aaaaaaatca ggatgctgca
     9241 tataaagaaa gcccggacgt gtcaaacgaa aactgtttcc agggggtcct ttaattcgtt
     9301 gacatcgggg cccccgtttt ttatagtttg cccggcaaac cggagccagc ccggcgcgcc
     9361 gcatctcccc tggagaacgc atgtgaaaac cagcggcaaa acggccctcc tgttcatgct
     9421 ctacctatcg cagggcctcc ccttcggctt ccaggccacg gcgcttcccg ttctcctgcg
     9481 ggaaagaggt ggttattcct caaacatccc caattgaaca aggcgataat cttccgtctc
     9541 ctgtcgcctg atataatcac ggactgtttt ctcatccgca ccaaccgttg atacaaagta
     9601 gccgcgagcc caaaaatgtt gacctgtaaa attctttttc cgccccatgt acgttctcgc
     9661 aatctgtacg gcacttttgc ctttcagata acctacaacc tgagaaaccg aatacctggg
     9721 cggcatcgat atcagcgtgt ggatatgatc gggcaaaaga tgcccctcca tcacttcgca
     9781 ctctttctgc gctgcgagat ccctgaacgt tgaccccaaa tacttccgca gctccacata
     9841 caacgtcttc ttccgatact tcggcatcca taccacgtga tacttgcatt cccatttcgt
     9901 atggggtaaa cacccatctc ccactgtttg cctcctatgc tgtaaacttt gagcggttca
     9961 cagcgggaag cctttcctgt agtcccggaa ttgtcaaact ctgggagtcc cccggcatag
    10021 ccgggggttt actcaggaga aattaaccaa ttggccttgg attagtgcca tcatgaaagc
    10081 ttaatggaat aaatacaaat gaccatgtat gttgtacaaa ccgttgatgt gaattggtgc
    10141 ccctggcgtg agtcgaacac gcggcccaca gattaggaat ctgtcgctct atcctgctga
    10201 gctacagggg caaccggatt tgatattccc taaagatgcg gcggtttaac attctttgcg
    10261 ggcgctgtca acctcccgcg ctttcaagcg ttcttgaaat ccttcaactt tcaaagaacg
    10321 tcttacaata agatgatgga taaaacgttt acagggacgc ctgccattca tcgcatcatt
    10381 aaaattgctg atccctggat cgaagtcagc cacagggtag caaaaaaatg cggcaaaagc
    10441 caagcaaatg tccaactgaa agaatgcatc tccttcggca tgctgacagt cttctccgca
    10501 atcaaaaagc cccctgttca gagaggctgc ggagaaatat ttgaaatggc cgaagtcgga
    10561 caagaaaagc cgggttgatg attttcttgt gtcgatgatt tccttgttga caggcgaagt
    10621 tgtttttctt atagtctggc gccaaaaata atctgatcaa aaaatagcag gtaaagaagc
    10681 gtgaccgaag aaactttact gatcgaagcc gtaccgctga aactggcctt gaaatcaact
    10741 gtccgtcata acgcggccgc caggaacgaa ggagaaagta tttgggtcca ggcaaaaaga
    10801 cggcacaaca gaggatatgg cgagggttgc ccgagggtct atgtggccgg cgacaacctg
    10861 gagtccagcc tccaatggat caaggaaaat ttttccaacg gtcaggtaaa ttttgcaacg
    10921 ttggaggatg tgcaacagtg ggcctgttgt catgaacaaa acatcgatcg ttacccatcg
    10981 gcatggtgcg ccgttgaaat ggcgatgctg gacctgctgg cccgggaaaa aaactgctct
    11041 gttgagaaac tgctgcgctt ggatgatttc aggggttacg gtcgctatac cgccgttata
    11101 ggagatgata aaggctggaa atatgccgcc ctggccgatc agtatctgat tcgcggactc
    11161 cgggatttca aaatcaagtt aagcggcaat ctggagcgtg acctggaaaa gatggatctg
    11221 ctggaaacat tatcgatgca gcatcaggtt ccttcccccc gtattcgtct cgatgccaat
    11281 aatttatgga agaatcattg tgacgaagcc gtcgcctaca cccaggccct gggaatcgga
    11341 cgcatattcg ccatggaaga acctgtggaa gcaggacatg tcgaagagat cagcaagttc
    11401 agcgtggcca cggggcttcc tgttattctg gatgagagtt tatgcaccct gaaggatctg
    11461 gataggttta aaactgcgcc aggtcagttc atcgctaata tcaagatatc gaaattgggc
    11521 ggtcttattc gggcgctgcg aatcatcgat gaggtcaaaa aactgggatg gcctattatt
    11581 atcggttgtc atgtcggcga aacttctttg ctgacccggg ccgcttttgc ggcctcctcc
    11641 gcggccggag agaacctgac agcccaggaa ggagctttcg gcgactatct ggtgaagcgg
    11701 gagccagtgg agccgatgct gaagtttgga cgaaacggga ttctggattt gcggtccccc
    11761 tactatctga aaaccgtcca gggactgaag gtcatcccgg ctgaaaactg gaatgtcgga
    11821 ttcggcatgc aaggtcatct gcctcattcc ccggacgacg gcgcgccgga catctgtttt
    11881 ctcgaaatgc ccgatcagta caagattcat tatcggcgct ggggccattc cgaaggtgaa
    11941 aatgtagcgc tgcttttaca cggagacatg gggcattcag gctggctggc cccgctggca
    12001 agtcgattac gttccctgtc accggatttg accgtcattg ccgccgatcg acggggctgc
    12061 ggattgaacg aacagcgggg cgacttgggt tccgtgcagg cggttattga agatgtcatc
    12121 caacacatca aatttctcaa aagatcgttc aaacaggtcc atcttgccgg ctggggacag
    12181 ggagcgcaat atgctgctgt tgccgggaca agaatgggca acgccgtctc cagtctgatc
    12241 ctgctgacgc cgggcttttt ctggaatgaa caattccagt ctgtttcaag gattaccaga
    12301 aaaattgtgc cggagatact tgccgaattc aaactgaaac ccgagcgcaa tcatgcctgc
    12361 attcccattc ccatggaagc gactgatttt acgctggaga atgaatggct tgatttcata
    12421 gaaagagatc ctctgaaaac cacgatggtc accttaaaat ccgttagcat catggatgaa
    12481 attcaggaac tttcatgggg cgccattcct caaaaccatc ttcccacact cgtgatgatg
    12541 ggccaacagg atcgcatcgt ggacaaccgt aaggttttgg aattcatcgg ccatcttttt
    12601 cagggcaaaa atcgcaaccg attggtcacg gtagaaagcg gccatgccat acactttgaa
    12661 aaaacggaag aagttgcttc cgaaatagta aattttatca ggaagctcta aaggttcaaa
    12721 ttaataatag gggagcggcg gaatgcaccc aaacggtcaa gaaacagtcg tgcagggcaa
    12781 ggacccctcc gagcatgtcc ttgcttcgat tccactgtgt ttccatcatg aagatctgat
    12841 ccggaaccct gatcctaaaa aggccatcag gaagagcaaa ctcatcaacc tgtggaatcg
    12901 ccttcatttt atggaggata tggtatatgt gcacctccat catcccgnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnntgg aatcgccttc attttatgga ggatatggta
    13081 tatgtgcacc tccatcatcc cgactatcat gagggtcttc ttctctgggc ctatccggaa
    13141 ccatgccaca acggtgtgat gacctgccgc tggccggagg ggagcggcac ttttaccgaa
    13201 aatgcggcga tattgaacat catcttctcc gatggactta cgttgtttct tatccctgcc
    13261 caactcaagg acgtccaaaa aaaatttttt gccatccagg cgcccgacag cgggtacatc
    13321 ctgggtcagc ggcgggccag gcgctatcgt tgccagggca tcaaagccga ggttgtacaa
    13381 aacggttttc acgccgtggg accgttgacg ggtttcagcg ctttgtcctt cgacgtcaag
    13441 gtcactccgg aggaaaccgg ttctttccgc tggtttaatg ccgacagttc atcgacggtc
    13501 aattggtatg cggatgaaca gatgatcttt tcggcaactt gccgctgcat ccggcaaaca
    13561 tcgaatcaaa cggcacggaa tatcgtcttt gcccctatgg taaaccggat gaaccgtttt
    13621 ccgaaaaaaa aatggcgcaa tcctcgtgtt caggtcaagc cttttccgaa tattgccttt
    13681 gaccatccgg ccgtcaacaa gagaattcaa ctggatattc atgatctctc ggtttcgggc
    13741 ttctccgttg atatttcgcc ggatgaggat gtccttacgg tgggaatgat cattcctgac
    13801 ctgaccatga attatgccgg cgctttgcaa atcaaatgca aggcgcaggt tctctatcga
    13861 cggatggaga aaaagaaaaa aatccgctat ggttttgtca tcctggacat ggatgtcgtc
    13921 aactataacc ggctgagtca tatggtcatg aatgttatcg atcccggcac tcatgtcgcc
    13981 gacgaagtcg atgtggatca actatgggag tttcttttcg acagcggatt catttacccc
    14041 aggaaatata atctggttca ggcctaccgg gagcccatga aagaaaccta ccggcggctg
    14101 tatcgcgata atccggaaat cgccacccag atcacttatc agcaaaacgg ccggatctat
    14161 gggcacgcct ccatggtcag atcttacaga cggacctgga tggttcatca tctggccgca
    14221 agacccctga acaataaaag aaccgggctt caagtcctga agcagattat gcattatttt
    14281 aatggtctct atcgtctccc atcggttggg atggactata tgatgttcta ttttcgcccg
    14341 gataatcagt ttcccgatca tttttttggc ggctttgcca ggcatttcag gaaccctcgc
    14401 gcctgttccc ttgacctttt tgcttatctc aattatccgg tgtccggtgt cggccggcaa
    14461 ctcccggcgg ggtggttgct cgaaccgtgc acgggcgaag actgggaagc gttaagccac
    14521 ttttatcgga atgcctccga tggtcttctg ctggatgtat tacgcctggg cgaagagaag
    14581 gaggatgggg aatctctgtc acaactctat gcccgccacg gattcatcag aagctgccaa
    14641 tgtttctctc taaagcaaaa cggcagatta aaggcggtgc ttattgtaaa ccgatcggat
    14701 ccgggcctca gcttgtcgga cttcctcaac gggatcaaga ttctgatcac cgatgcggcc
    14761 ggcttaccct gggaagtcct gtccacggcc gtttcccaat ggtcggattt ttataagagt
    14821 gacaaaatac cgctgcttgt ttatccatct cgttattttg aagaaaaagg agtttctttt
    14881 gaaaagtatt acaatctctg gattattgac ggtcattatg cccgggaata tagtgaatac
    14941 atgatggaaa attcgaaatt acgaatcaga tttcttatcc gctttctcat caagagatac
    15001 ctgaaaccat gatggacaac catactcata tctacaacag tcgcattatc aaaagctatg
    15061 tggagtatct gaagaagcat cttccggagg tcgatagcga tcccctgctg aaatatgcgg
    15121 atatcgaaac ctaccaactg gatgacgaag ggcactggtt gacccaggag caggtcgatc
    15181 gttttcatga tatccttgcg aaaacgaccc aggatcccga tatatccaga aaagtggggc
    15241 gtttctccgt tacatcccgc gcctccggcg ccctggggca atatatgctg ggatttatca
    15301 atccggccac ggcatatggc gttctggaaa gaatcaatgc ccagctaagc cgaggagcaa
    15361 ccctgaagac aaaagccatc gggacggaca ggattgaggc aaaagccact ttgcaggcgg
    15421 gtgtggtgga aaagccctat cagtgtcaaa accgattggg ggggctggag gcactggcaa
    15481 agctctccac caataaattt gccagcatag aacaccctgt ctgcatccat aaaggcggag
    15541 actgctgcct gtacgttatc tcctgggaaa acacccgcac ctttttctgg aaacgcatcc
    15601 gcaattatta cttttggatc gcatttgctc tttgcgccat cctgtttagt ttcatgaaac
    15661 cggcttactg ggatgtcctg accctatcgt gtgtgctgat gggaatgggc gtgacgctct
    15721 atgccgaaca tctggaaaag aaagatttgc ttgcccacat cttcaaccag ggagatgcgg
    15781 cggacagcct cctggatcag atcaacagac gttacaatga agccctcttg gtccaggaga
    15841 tcggacagaa atcctctgat atgaccaagg ttgatgaact cctccaatat attatagaat
    15901 cattggttaa aagactcgat ttcgaccggg ggatcatcat gctggccaat aaacaaaaag
    15961 accgcttagt gtatacggtg agttacggat ataatccgaa gaatgaagat tacctgcata
    16021 atattcaatt tcatctggac aatcctcaat caaagggtac ggctgtcgct tccttcagaa
    16081 cacagaaacc ttttttggtc aacgatctgt ccgacatcga gcatgacctg tcgcggaaaa
    16141 gtcttgattt cgtgaatacg atgggcagcc ggtctttcat ctgcgtccct atcgtctata
    16201 aaggggagtc tttgggggtc ctgattgtgg ataatgtcca gtcaaagaag ccgcttagcc
    16261 agaccgagat gagtctcctg atgggcattg ctccgcagat cgggattagc atcaagaatg
    16321 ctattgccca tcagaaaatc aaggagagcg aagagtgctt ccgctcgctc agtgaaaatg
    16381 ccccggacat tatttatacc ttgggcatca aagggaaatt tacttacatt aaccctgtga
    16441 ttgaaacgat cctgggatac cgccctgagg aaatcatcgg cagatacttt atcgatattg
    16501 tcagaaaaga agacgtgcac cggtgtattg aatttttcaa gcaagtcagg gatcatcaac
    16561 aaaccattaa ggaagaaatg ggcatcctgc ttcataagga tggcacggaa cggtatttca
    16621 gctttagttg tgcgcccaat tttgattccg aaagtcactt tataggggtg gtcgggacgt
    16681 tcaaaaatat gaccgacatc agaaaatcgg aaatggagtt gaaaaagagc ttcgagaaac
    16741 tgcaatcggt catgagcagt accattgatg ccatctccat catcgttgaa tcccgagacc
    16801 catacacggc aggccaccaa aggcgtgtga cacaactcgc aaccgccatc gcctgcgaac
    16861 tggctttatc caaagaaaaa atcgacctga ttcgtatggg cagtttgatt cacgacatcg
    16921 ggaagatata tattccctct gaaattttga caaaaccaat gaagctgaac gacatcgaat
    16981 atgccatgat taaatctcat ccggaggtgg ctcgaaagat attgaaacag gttgacttta
    17041 ttccaacggt tgtggatatg gtttatcaac accacgaaag gattgatggt tccggttatc
    17101 ccagaaaact aaaaagagac gacattctca tcgaggcccg gattcttgcc gtggccgata
    17161 cggtggaagc catggccaca catcggccct atcgccccag cctgggcatt gaaaaagccc
    17221 tggaggaaat agaaaatcaa agagacattc tttatgatga agctgttgcg gatgcctgcc
    17281 ttgtcttgtt tcgggaaaaa gggtttcaat ttaaagannn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnctg ttgcggatgc ctgccttgtc ttgtttcggg aaaaagggtt
    17461 tcaatttaaa gaggcctgag gatatgcgtc atccgccctg aggatattga aacgcttttc
    17521 gtatttacgc gctggcgaac tccttcaggt gcgtttccag cagacggtct ttgctgaaat
    17581 tcaccagttt gagcttttca ggcctggtgc ccgcaaaagc gcatttgggc gccagtccga
    17641 tcagctccga ctccagaatc tgcacaccgc gctcggcggc aagcgcctcc acccgttcat
    17701 aaaccgcttt gagcggcgtt tcccggcagg acgtcaggtt catggaaacc tgcgccagat
    17761 gcctgctctt caaaatgacg ccgatggcgc gcacatgcgg aaggccgccg cttttttcgc
    17821 ggatgagcga ggcgattttc tgcgccagcc tcagatcgtc gctgttcaaa ttgatgttgt
    17881 acgccaccag cggctcgcgc gcgccgacgg ccgtcgcgcc cgcgcccgga tgaaatacgg
    17941 gttcgccgac atccggcgcg tcgccccgaa tggacatctt ttcctttaga gcctcatagc
    18001 cgccgcgacg aacatccgca agctccctgc gccccggaac gagcgccgct tcgccgtaga
    18061 aataaaccgg aacgccaaag agctcatata accggttgcc gaagaggcgc gccagattca
    18121 cggcgtcttc cattttcgtt tttcccaggg gaatgaaggg aaccacatcc accgccccca
    18181 accggggatg aacaccggtc tgccgccgca tatcgatgac cttgagcgcc tgacccgccg
    18241 cggccagcgc cgcggccagc acatcttccg gtctccccag aaacgaaaaa acgctccggt
    18301 tgtgatcccg gtccccgccg taatccatca gacgaatccc tccggacgcc ttcaatacac
    18361 gggcgatgga atcgatcttc gcttcgtcgc atccttcgct gaaattcggc acacattcga
    18421 tgattttcat gtcgatacct gataaaattt taacgccggc aactgtacac aggtaaggct
    18481 gccgccgtaa accgcgccgg tgtcaatgcc gatcttattt tcggcaacca gcggcgcgtt
    18541 gaaatgcgta tgcccgaaaa ccacccgctt tccgaaatcg tcttcggaat cgatgaactc
    18601 ccgccggatc cactgcagat cccaggcgga ctgacaggca accggcgccc cgggacgcag
    18661 ccccgcatga acaaacagat actgatccgt ttcataatac ggcagaagcg attcaaaaaa
    18721 gctcaggtgt tcggcgggaa tctttccttt ccggacacgc ggggaatccg acaggaagat
    18781 tccgtagtcc cggagcgtcg cggcgccgcc gttttgcaga tacagatcct cctccacgcc
    18841 ttccagatag ttcaggagca tggattcatg attcccgcaa agacagacca atttggcaca
    18901 ggccccgcgg agtcccaaaa catagtccac cacacccttg ctttcgcggc cgcggtcgat
    18961 gtaatcgccg ataaaaacca gggtgtctcc tgccggatcg gccggaatct tccgcaaaag
    19021 ccgcttcagc ggatccaggc agccatgaat gtcgccgatg gcgaataggt tcatgatcct
    19081 ttgccgtctt gagcggccgg ctaatccaga agagcggccg taaaatcccc gctgtcgaag
    19141 ggccgcagat cctccagccc ctcgccgacg ccgataaagc gcaccggaag ccccagttcg
    19201 ccggcgatgc gcacgaccac gccgcccttg gaagtcccgt ccagcttggt cagcacaatg
    19261 ccggttgctc cgatttcttc ctggaacatt ttggcctgga tgacggcgtt ctggcccgtc
    19321 gtggcatcca gaaccagcag aatctcgtgc ggcgctccgg gcagctccct gccgatgact
    19381 cttttgactt ttttgagctc ttccatcaga ttggtccggg tatgcagacg tccggcggtg
    19441 tccacgatca ccacgccgtt aaaaccggct ttgattttcc cggcggcgtc gaaaacaacg
    19501 gccgcgggat cggcgttcat tttctgcttg atgaccggcg cgcccacacg agcgccccac
    19561 acctccagtt gttcgatggc cgccgcgcga aaggtatcgg cggcaaccag gacaacctcg
    19621 tgaccatccc ggcgcagcat atgggccagt ttgccgatgg tggtggtttt tccgctgccg
    19681 ttgacgccga cggccatgat cacaaacgcc tcgtttttcg ggatggcgag cggcttttcc
    19741 atctttcgca gaatggcgat catgcgctcc tgcaaaactt ttttaatctg cgacgcgtcg
    19801 gaaagctccc tgcgcttgac ccgctgcttg acgtcttcaa tgagatcaaa ggtaaactgc
    19861 gggcccatgt ccgccgaaat catcagctct tccagttcgt catagagcgc cggattcagt
    19921 tcccttttgc cgaaaacgag ctgatccaga tttttggtga gagcgttcct ggttttggcg
    19981 agtcccgctt tgagtttgtc gaataaaccc actttcttcg tattcctttc tgcaagcctc
    20041 cctgccggaa aataaaaggg ccaccgcacg gccgacaggg gaaaaccatt gcggccttta
    20101 gggagattca tagcggcttt cgccgccaaa gtaaagaaat ggatgatttt ctaaaggaaa
    20161 gggcgggggt tgccgcggtg caccgcgaaa aactcccgcc cgtcagaaag agagctttga
    20221 ataacggcca tttttgtcat ttcgaccgaa gggaaaaatc ttgaacaaac ggccatcctt
    20281 aagatttctc gtcgctcacg ctcctcgaaa tgacattttt caaagccctc agaaacagac
    20341 atggtccgga tgccgcccgt ttctcccgcc ggggcggatg tcggacgggc ccgccgccgg
    20401 attcaggggc gccggaccgg acaaaatgct atttaaatac cggccgctgg ggcatcagct
    20461 tgatatattt gatctctgct ttggtctctt cgtactgaaa tcccaggata tcccgcatcg
    20521 aaatcatgcc cactaccctt ttgccgtccg caatcggaat atgccggatc tttttatgtc
    20581 tcatggtatt gatcgcgtaa tccagatcgt catccatcga cgcgatggcg acttcatgcg
    20641 tcatgatatc cccgactttc ttgtcgtcgc aattccctcc gcgcgcgagg cacttgcgca
    20701 cgatgtctct ttccgtaatg atgccgatca actcattttt ttcatccgtg acgggaagcg
    20761 cccctttatc gtgctggatc agtttctgga tcgccgctga aacaaattca tccgcctcga
    20821 cggtgattaa agattgactt ttgaatcccg tagctttcaa catgtcccgt atcttcatct
    20881 cccttttcct cccttgttcc atatgctctt gagcaggtta agaatcgatt tctgaacgat
    20941 aattccttta acgacggttg aaaaacaaaa atcgcctcct gaaaaatttc gtatctttca
    21001 tatcactcat ttttttccga aatcaagcac tttcttcaaa acgacgccga agaggacttt
    21061 cggaatgggc cgaaaatctc ccgcgaacaa acaacgccct tgcctgcggc gggggaatcc
    21121 gcgccgaaag cgatagaacg ggccggat
//
LOCUS       DAOSRM010000029        20948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2721_length_20948_cov_86.421624, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000029 DAOSRM010000000
VERSION     DAOSRM010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 20948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20948
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2721_length_20948_cov_86.421624"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLA58_04490"
     CDS             <1..584
                     /locus_tag="PLA58_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOR62085.1"
                     /translation="TYINSTAEVKAESDICCTSSNAIKIVQNLPPSKKVVMLPDGNLA
                     RHAASVTGRDIAAWEGCCPIHQALTAQEVAARRREHPRAPFAAHPECRPEVLALADFV
                     GSTSAILQFARDTKANEIIIGTEKGILTQLTKDNPEKTFIPASDALFCADMKKISLDD
                     IERSLATLQPVITVPRDVSEAAKKALDKMLALS"
     gene            616..4170
                     /gene="mfd"
                     /locus_tag="PLA58_04495"
     CDS             616..4170
                     /gene="mfd"
                     /locus_tag="PLA58_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOR62086.1"
                     /translation="MRKIIDKKMVPDAPPVRELLEGIAKGAPVTDARGLVPPARAFLT
                     ALLFERLAAPLIVLCPTAKEAASFARDLALFLGEGAVLHYPPLDFLSVDMFALQREEE
                     MARLHVLTQLQVDSRKIVVTSLAACMQKVMPYAEFQQYLKILSIGDVLLMEEFPQKLA
                     AGGYSRVSLVENPGEFSLRGNILDIFPPGAPDPLRLEMAGDEIESIRRFDAASQRSGA
                     SVDAFVLGPAGEILLDEERLNRAVRNVKRRADDLDLPRELRSRLSETLREQSATSLNP
                     IFLPLFYEEYDDPAGFGRRNLSSVFDYLPRNSLFVINDPLGVEQAQQDVSAGIDKLLY
                     KAKAGGRFYLEHAGLYLDEEPLRKRMDDFQQVWFSGLTLDDPKSAVPVVNFDTRPEVP
                     VPGTSFGDGKEESHLGRTAEKIQGWLAQGRPVFFVSPTPEDTARMQHLLAGYGLPARM
                     LSPEAPLLEIIQNPGEGSVLFLREGKLSGSFVLESPGLAFLSEEDIFVKKTPRRRVRP
                     RHEGYFLKSFGDLKEGDFVVHRDFGIGLYRGLQKIAVGAVENDFLVIEYLDGDKLYLP
                     VNALEKIQRYLGPDGYTPKIEKMGGTSWDAVKERVKKSVRDVAEELVAIYAAREAMER
                     KPFLPPDRVYEEFCSTFEYEETPDQARAIEDIHADMDDVKPMDRLICGDAGFGKTEVA
                     LRAAFRAAMDGKQTALLAPTTILTEQHFATFSRRLADFPIRVEALNRFRSPAEIKKTL
                     ADLLRGRVDIVVGTHRLLQKDVAFKNLGLVIIDEEQRFGVAHKEKLKKMRTLVDVIAL
                     SATPIPRTLHLSLTGIRDLSIINTPPEDRRPIKTYVLEFDEDVIRGGIEAEIARGGQV
                     FFVHDRVRSIYGIANLAQRLVPRARVGVVHGQMKPAEIEKAMARFIRREFDVLVCTTI
                     IGSGLDIPSANTIFINRADRFGLAQLYQIRGRVGRGSEDASAYLLLPRGALLSRDALR
                     RLQAIKEFSEPGSGFRIAHNDLEIRGGGNVLGLSQAGHISAVGYELYTELMEKTVREI
                     QGAPALVEEALPEIQMGISAFLPEDYVQDVHRRLVLYKRISLAAGPEDLQDIRAELAD
                     CYGPLPQAAENLLRVIGVRNLLAPLKARKMGFDGKYFYIYFSEASPVDPARITALTRK
                     KIRDLRFTPDFRLFLPARPAGAPDLLDRAEELLKMLAE"
     gene            4435..5412
                     /locus_tag="PLA58_04500"
     CDS             4435..5412
                     /locus_tag="PLA58_04500"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOR62087.1"
                     /translation="MKALLQNLVRQWRTLAPLAVLCALCLVSACGRQEAGRPHVATVN
                     GEKIYLEEYRARLGDRKGLFSLRALQGESGKRMRLEEEILDALITEKMVLQRARELGL
                     SVSETELENRLLEIRGDYGENYFDLLVRKNVRYEDWREALKKEMLFEKLVAAEVHAAV
                     RVSEDEAEDYFREHPDFCRSGARVRASQIVTPDLDQAREVEERLERGEDFALLAKAFS
                     IGPEAVRGGDLGLIARDTMPEPLDKTLFTLPLHKTSPIVKSAYGYHILKVTDIQPARA
                     RSFAECRQELLDVLRAQKEEAAFNAWLNNLKMKAVVKKEINVLRENTPS"
     gene            5437..6414
                     /locus_tag="PLA58_04505"
     CDS             5437..6414
                     /locus_tag="PLA58_04505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR62088.1"
                     /translation="MKAYKTGFAVILAVLLISAPAAAGVADRIVAVVNGEVITLSELH
                     RAFAPYAAHIEANYKGPDKEAFLKQGEAAFLQRMIDQILIEQEAKKPGVGIAAVKDEE
                     VMGMVKDMLAKNRLTMQDYLKKLAEEGKTLESAKQEIRGQMLRMRLLRREVQSRILVT
                     DEEIGEYYDKHREDYEGREAVHIRQIFLPVPEGADSGARDRVRAEANQLRERILKGER
                     FEVMAARYSRGPAAAEGGDIGFVERGVMMPEVEKAAFSLPVGEVSEVLETEAGFLLLV
                     VVDKKGAGLKPLPVVRDEIKAKIEDEKVNKKYDEWMAELRKKSFIDIRL"
     gene            6433..9237
                     /gene="uvrA"
                     /locus_tag="PLA58_04510"
     CDS             6433..9237
                     /gene="uvrA"
                     /locus_tag="PLA58_04510"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOR62089.1"
                     /translation="MDLIRIKGASQHNLKNISLDIPRDRLVVITGVSGSGKSSLAFDT
                     IYAEGQRRYVESLSTYARQFIGQMDKPDVESIEGLSPAIAIEQRAASHNPRSTVGTVT
                     EIYDYFRLLFAGIGVCHCSNCGREIQSQTIDSIMQSVLDLPEGTRFSILSPLVRGKKG
                     EFAKELKKLQKEGFARVRIDGEIRDLSLDLVLSKTKRHDIDLIVDRLVMKDGIRKRLR
                     DSLEIAVHKGDGLVRIAAADGGETLYSEKYACPDCGISMPPLAPRVFSFNNPYGACPE
                     CNGLGSRMHFAPDLVVPDAGLSLREGAIAPWANRSSLYYFNLLDTLAEHYDFDINMPF
                     SNLPEKIRKVLLYGSGTEQIKFHIDRPDKRYFVHRPFEGVIRQLERRWRETASAGVRS
                     DLARYIDLRPCETCGGARLKKESLAVTVGGRNIYELCLMSIRECFEFFEGLKLTGQET
                     KIVERVLKEIKSRLSFLLNVGMDYLNLARSTSTLSGGESQRIRLATQIGSGLMGVLYV
                     LDEPTVGLHQRDNLRLIDTLKKLRDMGNTVLVVEHDADMMLACDHIIDMGPGAGARGG
                     EVIFQGSPAEVLQSETSLTGQYLSGRKSIGLPAWRKVKGRHIVLEGATENNLKNIDIR
                     IPTGVLTAVTGVSGSGKSTMVIETLYQVMARRLNQHSGPMAKIRRVRDMGGIERVIMI
                     NQQPIGRTPRSNPVTYTGIFSFIRDLFTGLPDARIRGYKPGRFSFNVKGGRCEACEGN
                     GLIKIEMHFLPDVYVTCDACRGKRFNADTLDIKYKDKSIADVLDLTVSQALDFFANIP
                     SIRSHLQLLADVGLGYIRLGQSATTLSGGEAQRIKLARELGKRVNQNTLYILDEPTIG
                     LHFADIQKLLDVLMRLVDMGNTMVVIEHNLDIIKSADHIIDLGPEGGPGGGRIIASGT
                     VREVARNEQSFTGAFLKKVL"
     gene            9254..10390
                     /locus_tag="PLA58_04515"
     CDS             9254..10390
                     /locus_tag="PLA58_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOR62090.1"
                     /translation="MNSIPLVPLKYRHSILLIDDEEAVLEIMTMALADEGYDLRTAGS
                     AEEALAILKESPDVSLIISDQLMPGMTGVQFFTQARHICPDALRVLLTGYTDTDAIID
                     AINSGGIHRYLTKPWRREELLHTIREMLGKADLVMENRRLDELVKKQNAELMELNKSL
                     EEKVRLKTKHLNRSLEELQVSYEKTRMILEGTVLAMSRIVESRDPYTAGHEQQVAKIA
                     CVISEKMLLPADRVDAIRVAGQLHDIGKIAVPSEILTKPGRLSPLEMEMVKTHCRNAY
                     DILKAIEFPHPVAKIILQHHERMDGSGYPQGLKGDDILLEARIIGVADVIEAISSHRP
                     YRPAKDVDVAMQEITDHRGTLYDANVVDAALDVYRTKGRQDFLP"
     gene            complement(10430..11074)
                     /gene="nadD"
                     /locus_tag="PLA58_04520"
     CDS             complement(10430..11074)
                     /gene="nadD"
                     /locus_tag="PLA58_04520"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000839.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOR62091.1"
                     /translation="MNLGLFGGTFNPVHLGHLRAAQELAELLKLDRIVFIPAATPPHK
                     TPSGIVSFEHRLQMLQLAVAGNDVFSLSDVENRRPGKSYSIDTVRHFRNAGDSGLECF
                     FITGQDAFDAITTWHDWKNLLRLCHFVVMTRPGYENRGLHQILPPDVAGRYVYDAAGD
                     VFQQPHGGSIFFRQTTFLDISSSEVRKRMQQGRSLRYLTPDSVIRYMEANRLYR"
     gene            complement(11071..12327)
                     /locus_tag="PLA58_04525"
     CDS             complement(11071..12327)
                     /locus_tag="PLA58_04525"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOR62092.1"
                     /translation="MNIADSVKKIAADALAASRLLSRCPTGEKNEVLTRMAEELLRNR
                     DRILSANQRDVTAAREKGLSAAMIDRLTVKDATLAAMARGLREVAALPDPVGKVTSMW
                     RRPNGLLVGRMRIPLGVIGIIYESRPNVTVDASALCLKSGNAVILRGGSEAIHSNLAI
                     ASVLQEALQKSPLPDHAIQVIPFTDREAVSILLSLDEQIDLIIPRGGEELIRAVVSQS
                     KIPVIKHYKGVCHIFVDASADLDMAVNICANAKVQRPGVCNAMETLLVHADIAPVFLP
                     KVAERLQKDGVRLKGCEATGKILKGIEAATEEDWTAEYLDLILAVRVVASIDEAIAHI
                     EKYGSLHTESILTKDYANAQRFLNEVNSSTVLVNASTRFSDGFELGLGAEIGISTTKL
                     HAYGPMGLEELTTSKFIIYGDGQVRT"
     gene            12646..13713
                     /locus_tag="PLA58_04530"
     CDS             12646..13713
                     /locus_tag="PLA58_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62093.1"
                     /translation="MSRLRIKAGRKAYEIIRDGGFHFDAVSAYFGAAVGPRWLVASGF
                     DLTLLTGDLLGRSRPVHLIGSSAGAWRFAAWIQPQAADSYRRFMNAYISARFTRQDTP
                     ETILGKFADILGSYIEDDALSFALANKRYRLSVITARSRGLVASEMLLLQKSALAACF
                     ALNFVSRSQLYRFAERVVFYNGSKPPPFCFQPSFRGRYVRLNEINFKHAVMASGAIPL
                     VVAGVKNIFGAPDGVYRDGGLIDYHLSHQFAGKEGDLVLFFHHEERIIPGWLDKKIKR
                     RAPDEQTLDNVVMVFPSQSFVDRLPDGKIPDRDDFLTHIDNPGRRIENWNRAASLSAP
                     LGEEFLDLVESGRIRDIVEKM"
     gene            complement(13744..14595)
                     /locus_tag="PLA58_04535"
     CDS             complement(13744..14595)
                     /locus_tag="PLA58_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOR62094.1"
                     /translation="MSRPLRIEYPDAVYHVTTRGNAKNDIFYSDSDREAFLKILSGVV
                     SRFNWLCHAFCLMDNHYHLLIETPDGNLSKGMRQLNGVYTQKYNWMHQKEGHVFQGRY
                     KAILVEKESYLLELCRYVVLNPVRAGMTEKPEQWRWSSYRFTAGMKTPPAYLTTGWIL
                     ELFHQKKTEAEKLYQKFVTAGIGLKSPWKELRGQILLGEEEFVDSHRDLLQDKQTVKE
                     VSRIQRYLSRPKLSTLFLKERNKTERDKAIHRAHIQHGYTLKAIADHLGIHYTTVSKV
                     IKTPSEN"
     gene            complement(14651..15292)
                     /locus_tag="PLA58_04540"
     CDS             complement(14651..15292)
                     /locus_tag="PLA58_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOR62095.1"
                     /translation="MMIAFDLHIHTRRYSGCSFIEYEDLVGQAASAGLSGFALTEHGM
                     RWPDAEFGRLRDIAAERGMLLINGQEINTFDASHRSEGEFLVFGLSRSLTKECPAAEL
                     VHTVHEEGGIVIAAHPYKLARGGKSHYYGAGDRVYQLKLDAIELCHPDHGESAMKKVR
                     RAMEELKIPGTGGSDAHKILQVGSCVTVFENPVRNEDDFIREIRAGNVTGVRS"
     gene            complement(15518..15742)
                     /locus_tag="PLA58_04545"
     CDS             complement(15518..15742)
                     /locus_tag="PLA58_04545"
                     /inference="COORDINATES: protein motif:HMM:NF040111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HOR62096.1"
                     /translation="MKKKSRDLEKTYPVAEFAAKLRRLADALENGKRFIITIAGERIS
                     VPSRATFNVEHEREGKNEEIEFQIKWTNKN"
     gene            16164..17864
                     /locus_tag="PLA58_04550"
     CDS             16164..17864
                     /locus_tag="PLA58_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOR62097.1"
                     /translation="MAGEVSYASKPWLKSYDKGVPEKIQYEEICMPDILDRSAAQNPD
                     RMALNFQGYTMTYKELKDMVDRFAAYLAGVGIKKGDAVAIILPNMIPTVVAYYAIHRL
                     GGIVVLNNPTYSDRELEHQLNDSGAKIMIIIDLLANRMIDLRPKTKVKQIIITSIGDY
                     LPFPVNLLFPLVGKKKGLLPAKPIKPANDVFRWKDCIAKSQPNPPKVKLTFDDTAMYQ
                     YTGGTTGVSKGVILTHGNISKQVQQIRAWFPKFAGTYNVNLGALPFFHVFGMSCAMNF
                     SVIQGWGDILIPKPQPEPLLEAIRKFRPNFAALVPTMFIGMLNHPKMKTTDMTCFKGF
                     FSGSAPLPVEVIHDFEKTAGSVICEGFGMTETTPVTHTNPFEGIRKIGSIGLPVSDTE
                     CRIVDLDKGETDMPVGEPGELIIRGPQVTKGYLNKPEETAGLLRDGWCYTGDIATMDQ
                     DGYFYIVDRKKDMIISGGFNVYPRDIDEAYYEHPKVMEACAIGIPDAKRGENVKLFIV
                     LKEGETATAEEMIEYGKSKLATYKLPTEVEFRKELPKSTVGKVLRKELRAEELAKRKK
                     "
     gene            complement(18078..18473)
                     /locus_tag="PLA58_04555"
     CDS             complement(18078..18473)
                     /locus_tag="PLA58_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOR62098.1"
                     /translation="MKILVAYVGGLDVDRAVLEVAKKHAKAFNASLYVASSMERVSER
                     ERRDLDKVEKHLAYVKETMDAEGIECETHILVRGLTPGEDIVEFAEDQKVDEIIIGIE
                     KKSKVGKLLFGSNAQYIILQSSCPVVSVK"
     gene            complement(18598..19890)
                     /locus_tag="PLA58_04560"
     CDS             complement(18598..19890)
                     /locus_tag="PLA58_04560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR62099.1"
                     /translation="MIHSSPSTQPAFSGWTTLAGVMLAYGAICGNVTYAFGVFLPSMG
                     ESFGWSRSALSGPYTLFLIIGGMLGPLAGVTVARFGARKNIVLGNVIAALGLLGMSQV
                     REIWQVYLFFGVMGGLGLAFAEFLSATTVINHWFIRKRSLALGFLFASGGIGGFLMPP
                     LISMLISGLDWRFAWAALACLHLIMGVAGAGLLIRNMPEEESQFPDGHASKEQFDEWR
                     EKATGHVYHTREDWSVRDALRTPALWILLALFSVLLFATTLLTTHQVAYLQDLKHSPM
                     VSATALGLMLGMSILGRLLSGILGMRFSGRHLAAVFMACLGFGLLSLMNAREIVFVYL
                     YSILTGLGFGGMIVLLPHTMGAYFGRANFSRIVGWTTPIVTLASAASPLLAGFLYDST
                     GSYTLPFAVTTALIFVCCILALAARPPKPRDKTSAPRA"
     gene            20079..>20948
                     /locus_tag="PLA58_04565"
     CDS             20079..>20948
                     /locus_tag="PLA58_04565"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOR62100.1"
                     /translation="MTGSDVLVVVGHSGVVIPPEISLEDLTDEFTALLKNVDWYTQWL
                     YDFRDILGNRQLVFPCCSILLDANRDPADLDEAVPVRDVFGRPIYRSAYEPSPSMRAA
                     WSDKYLKPFHRGIEENISAGAGLLFDGHSTVTARGVAANQIDLMNFQHTDREEKALYY
                     CPDVIVETYAEELRKRLPDALVTVNASEYVAVHGHICAAHSVNALKRVGARAPAFIQE
                     TNENLYKNSDGTPNVGQINRLRRAFAESLAQTLQSLQESQKVTMIDLHLGKQVYDYDC
                     GVQALQTVMTYYGV"
BASE COUNT         4653 a         5825 c         6057 g         4413 t
ORIGIN      
        1 tgacgtacat caactccacg gcggaagtga aggccgaaag cgacatctgc tgcacgtcgt
       61 ccaacgcgat taaaattgtt caaaacctgc cgccgtcgaa aaaagtggta atgctgcccg
      121 acggcaatct ggcccgccac gcggcgtccg tcacgggtcg ggacattgcc gcgtgggagg
      181 gctgctgccc gattcatcag gcgctgacgg cccaggaagt tgcggcccgc aggcgggagc
      241 atccgcgggc gccgtttgcc gcgcacccgg agtgtcgtcc ggaagtgctt gcccttgcgg
      301 atttcgtggg gtccacgtcg gccattctcc agtttgcccg cgacacaaaa gccaatgaga
      361 tcattatcgg aacggaaaaa ggcattttga cccaactcac caaagacaac ccggaaaaaa
      421 cttttattcc cgcctcggac gcgcttttct gcgccgacat gaagaaaata tcgctggacg
      481 atatcgagcg gtcgctggcg accctgcagc cggtcattac ggttccccgg gatgtgagcg
      541 aggccgcgaa aaaagccctg gacaaaatgc tggccctttc ctgaccgacg gtttgattcg
      601 cctggatgac atttcgtgag aaaaattatc gataaaaaaa tggttcccga cgccccgccc
      661 gtgcgggagt tgctggaggg gatcgcgaag ggagcgccgg tcacggatgc gcggggtctg
      721 gttccccccg cgcgtgcgtt tctgaccgcg ctgttgttcg agcggctggc cgcgccgttg
      781 atcgtcctgt gtcccacggc aaaggaagcc gcttcttttg cccgagatct ggcgctcttc
      841 ctgggcgaag gggcagtgct gcattacccg cccctcgatt ttctttcggt ggacatgttc
      901 gccctgcaga gggaggaaga aatggcccgc ctccatgtgc tcacgcaact gcaggtggac
      961 agccgcaaga tcgttgtcac ctctctcgcg gcctgcatgc agaaagtcat gccctacgcc
     1021 gaatttcaac aatatctgaa aattctttcc atcggcgatg tgctcctgat ggaggagttc
     1081 ccccaaaagc tcgcagcggg cggatattcg agggtatcgc tggtggaaaa tcccggcgag
     1141 ttcagcctgc gcggcaatat tctggatatt tttccgcccg gcgcgccgga ccccctgcgc
     1201 ctggagatgg cgggcgacga gatcgaatcc atccgccgct ttgacgccgc gtcccagcgc
     1261 tcgggcgcgt ccgtggacgc gtttgttctg gggccggccg gcgaaattct cctcgatgag
     1321 gaaaggctca accgggccgt gcgcaatgtc aagcgccgcg ccgacgatct ggaccttccg
     1381 cgcgagttgc gcagccggct ttcggaaacg cttcgggagc aatccgcgac ctctctcaat
     1441 ccgattttcc tgcccctgtt ttatgaagag tacgacgatc cggcgggctt cggccggcgg
     1501 aatctctcca gtgttttcga ctatcttccg aggaattcgc tgttcgtgat caacgacccg
     1561 ctgggggtgg aacaggcgca gcaagatgtg tcggccggca tcgacaagct tctgtacaag
     1621 gcgaaagcgg gcggcaggtt ctatctggag cacgccggtc tttatctgga tgaagagccg
     1681 ctccgtaaac gaatggacga ttttcaacag gtatggttct ccggcctgac gctggatgac
     1741 ccgaaaagcg ccgtccccgt ggtgaatttc gatacccgtc cggaggttcc cgttcccggg
     1801 acttcgtttg gcgatgggaa ggaagagtct cacctcggcc gcacggcgga aaaaattcaa
     1861 ggctggctgg cgcagggaag gccggtgttc tttgtgtctc ctacgccgga agacacggcc
     1921 cgcatgcagc acctgctggc ggggtatggt ttgccggcgc ggatgctgtc cccggaggcc
     1981 ccccttctgg aaatcatcca aaatcccggt gagggatccg tgctgttttt gcgggaagga
     2041 aaactgtccg gcagcttcgt tctggagtct cccgggctgg cgtttctgag cgaagaagat
     2101 atttttgtca aaaagacgcc tcgccgccgc gtccggccgc ggcacgaggg atatttttta
     2161 aaatccttcg gcgatctgaa agaaggggat tttgtggtcc accgggattt cggcatcggg
     2221 ttgtaccgcg ggctgcaaaa aatcgccgtg ggcgccgtgg aaaatgattt tctggtcatc
     2281 gagtatctgg acggcgacaa gctctatctg cccgtgaacg ctctggagaa gattcagcgc
     2341 tatctggggc cggacggcta tacgccgaaa attgaaaaga tgggagggac ctcctgggac
     2401 gcggtcaagg aaagggtcaa aaagtccgtc cgcgacgtgg ccgaggagct ggtggcgatt
     2461 tatgcggcgc gcgaggcgat ggagcgcaaa cccttcttgc cgcccgatcg cgtctatgag
     2521 gaattctgct cgacgtttga atacgaggaa acccccgatc aggcgcgggc cattgaagac
     2581 atccacgccg acatggacga cgtcaaaccg atggaccgcc tgatttgcgg cgatgcggga
     2641 ttcggcaaga cggaagtcgc gctgcgcgcg gcgttccggg cggcgatgga cggcaaacag
     2701 acggcgcttt tggcgccgac cacgattctg accgagcagc atttcgcgac gttttcgcgt
     2761 cggctggcgg attttcccat tcgcgtggaa gcgctcaacc gcttccggag tccggccgaa
     2821 atcaaaaaaa cgctggcgga cctcctccgg gggagggtcg atatcgttgt cggcacgcac
     2881 cgccttctgc agaaggatgt ggcgttcaaa aacctggggc tggtgatcat cgacgaagag
     2941 cagcgcttcg gcgtggcgca caaggaaaaa ttgaaaaaaa tgcggacgct ggtggacgtg
     3001 atcgcgcttt cggccacgcc gattccgcgc acgctgcacc tgtcgcttac gggcatccgc
     3061 gacctgtcga tcatcaacac gccgccggaa gaccgccggc ccatcaagac ctacgtcctg
     3121 gaattcgacg aagacgtcat tcgggggggg attgaggcgg aaattgcccg gggcggccag
     3181 gtgttttttg tccatgaccg ggtgcgctcc atttacggga tcgcgaattt ggcgcaacgg
     3241 ctggttcccc gcgcgcgggt cggcgtcgtg cacggccaga tgaagccggc ggaaattgaa
     3301 aaagccatgg cgcggttcat ccggcgggaa ttcgacgtgc tggtttgcac gacgatcatc
     3361 ggatcggggt tggacatccc gtccgccaat acgattttca tcaaccgcgc cgaccgcttc
     3421 ggcctggccc agctctacca gattcgcggg cgcgtgggtc ggggcagcga ggatgcctcg
     3481 gcctatctgc ttttgccccg cggcgcgctc ctctccaggg acgctctgcg caggcttcag
     3541 gccatcaagg agttttccga accgggctcc gggttccgca tcgcccacaa cgatctggaa
     3601 atccgcgggg gcggcaatgt gctgggcctt tcgcaggccg ggcacatctc cgccgtcggg
     3661 tatgagcttt acaccgaact gatggaaaag accgtgcggg aaattcaggg cgctccggcg
     3721 ttggttgaag aggctcttcc tgaaattcag atgggaattt ccgcttttct gccggaggac
     3781 tacgtgcagg atgttcaccg gcgcctggtc ctgtacaaga gaatatcact cgcggcgggt
     3841 cccgaagatc ttcaggacat ccgcgccgag ctggcggact gctacgggcc cctgccgcag
     3901 gcggcggaaa atctgctgcg tgtgatcggc gtccgcaatc tcctcgcgcc gctcaaagcc
     3961 aggaaaatgg ggttcgacgg aaaatatttt tacatttatt tcagcgaagc ctccccggtg
     4021 gatccggccc gaattaccgc gctgacccga aagaaaatca gggatttgcg ctttacccct
     4081 gacttcaggc tgtttctgcc ggcccgcccg gccggcgcgc cggatcttct ggaccgggcg
     4141 gaggagcttc taaagatgct tgccgaatag cctttatcgg gatatggata aagacatttt
     4201 ccggcgcggg agatccggag cgccaaaata ggcgcggcgg gaatgacatt cttgatgaac
     4261 tggtaaaaag tcaatttcgg caccttttcg tcataccgac gaaggccggt atccagtaag
     4321 tacagtgggt tatggacccc ggccttcgcc ggggtgacgg ctcgaattac tttttacgag
     4381 accgtcattc ttggttttct gtagtgatgc aaacgttcgg ggaaacggcg cgtgatgaag
     4441 gcgcttttgc aaaacctggt tcggcaatgg aggacgctgg cgcccctggc cgtgctttgc
     4501 gccctttgcc ttgtttccgc ctgcgggcgg caggaagccg ggcgcccgca cgtcgcgacg
     4561 gtcaacggcg aaaaaattta cctggaggaa taccgggcgc ggctgggcga ccgcaaggga
     4621 ctgttttcgc ttcgcgctct tcagggcgaa tccggcaaac gcatgcgcct ggaggaggaa
     4681 attctggacg cgctgattac ggagaaaatg gttctccagc gggcgcgcga gctgggcctt
     4741 tcggtcagcg agaccgagct ggaaaacaga ctgctcgaaa tccgcggaga ttacggcgaa
     4801 aattattttg atttgctcgt ccggaaaaat gtgcgatatg aagactggcg cgaagcgctg
     4861 aaaaaagaga tgctgttcga aaagctggtg gccgcggagg tccatgcggc cgtccgcgtt
     4921 tcggaagatg aagcggagga ttatttccgg gagcatcccg acttctgcag atccggagcg
     4981 agggttcggg cgtcccagat tgtcacgccg gatctcgatc aggcgaggga agtcgaagag
     5041 aggctggaga ggggtgaaga cttcgccttg ctggccaagg cgttttccat cgggccggaa
     5101 gcggtccgcg gcggggacct ggggctgatc gcgcgcgata ccatgccgga accgctggac
     5161 aagacgcttt ttactctgcc gcttcacaaa acgagcccca ttgtcaaaag cgcctacgga
     5221 taccacattt taaaggtcac ggacattcag ccggcccggg ctcgatcctt tgcggaatgc
     5281 cggcaggaac ttctggatgt tctccgggcg caaaaagaag aagcggcttt caacgcctgg
     5341 ctgaacaact tgaagatgaa ggccgtcgtg aaaaaggaaa taaatgttct cagggagaac
     5401 acaccatcat aacgatcgtt caacaggggg aactacgtga aggcttataa aacgggtttt
     5461 gcagtcatcc ttgccgtttt gctgatcagt gcgccggcgg ccgccggcgt ggcggacaga
     5521 atcgtcgccg tcgtcaacgg cgaagtgatc acactttctg aactccaccg ggcatttgcg
     5581 ccctatgccg ctcacattga ggccaactat aagggcccgg acaaggaagc gtttcttaag
     5641 cagggcgagg cggcttttct gcagcggatg attgatcaga tcctcatcga acaggaggcg
     5701 aaaaagccgg gcgtcggcat tgcggccgtc aaagatgaag aagtcatggg catggtcaag
     5761 gacatgctgg ccaaaaaccg gttgaccatg caggactatt tgaaaaaact ggccgaagaa
     5821 ggcaagacgt tggagagcgc caaacaggaa attcggggac agatgctgcg catgcggctt
     5881 ctgcgacgcg aggtgcagtc gcggattctg gtgacggatg aagaaatcgg cgagtattac
     5941 gacaaacatc gcgaggacta tgaaggccgg gaagcggtgc acatccggca gatttttctg
     6001 ccggttccgg agggagcgga cagcggagcg cgcgaccggg tccgggccga ggcgaatcag
     6061 ttgcgcgaac gcatcctgaa aggcgagcgc tttgaagtga tggctgcgcg ctattccagg
     6121 ggcccggccg cggctgaagg aggcgacatc ggctttgtgg aacggggcgt catgatgccg
     6181 gaagttgaaa aggcggcctt cagccttccc gtgggggagg tcagcgaagt tctcgaaacc
     6241 gaagcggggt ttctcctgct ggtggtcgtg gacaaaaaag gggcggggct gaagcccctg
     6301 cccgtggtgc gcgatgaaat caaggcaaaa attgaagatg aaaaagtcaa caaaaagtat
     6361 gacgaatgga tggctgagct ccgcaaaaaa tccttcattg acatccggct ttaagaaacg
     6421 gcaattccgg ccatggattt gataagaatc aagggcgcgt cccagcacaa tctcaaaaat
     6481 atttctctgg atattccccg cgaccggctg gtggtcatca ccggcgtttc cggctcgggc
     6541 aaatcctccc tggcgtttga caccatctac gccgagggtc agcgccgcta tgtggagtcg
     6601 ctttcgacct acgcgcgcca gttcatcggc cagatggaca agccggatgt ggaatccatc
     6661 gagggcctct cgcccgccat tgccatcgaa cagcgggcgg ccagccacaa tccgcgctcc
     6721 accgtgggca ccgtcacgga gatttacgat tatttccgcc tgctgttcgc cggcatcggc
     6781 gtctgccatt gcagcaactg cggtcgggag atccagtcgc aaaccatcga cagcatcatg
     6841 caaagcgtgc tcgacctgcc cgaagggacg cgcttcagca ttttatcccc gctggtcagg
     6901 ggcaaaaaag gcgagtttgc caaagaattg aagaagctcc agaaagaagg ctttgcgagg
     6961 gttcgcattg acggcgaaat ccgcgacctg tcccttgacc ttgttctttc caaaaccaaa
     7021 cgccacgaca tcgatctgat cgttgaccgc ctggtgatga aggacggcat ccgcaagagg
     7081 ctgcgggact ctctggagat tgccgtccat aaaggcgacg ggctggtccg gattgccgcc
     7141 gctgacggcg gagagaccct gtattccgaa aaatatgcct gtccggactg cggcatcagc
     7201 atgccgcctc ttgccccccg cgtattttcg ttcaacaacc cctatggcgc ctgtcccgaa
     7261 tgcaacggtc tggggtcgcg gatgcacttc gcgccggatc tcgtggtgcc cgacgcgggc
     7321 ctttctctgc gcgaaggcgc cattgctccc tgggccaacc gcagctcgct ttactacttt
     7381 aacctgctgg acacactggc ggaacattat gatttcgaca tcaatatgcc gttttccaat
     7441 ctgccggaaa aaatccgcaa ggttctgctg tatggatcgg gaacggaaca gatcaaattt
     7501 cacattgacc gtccggacaa acgctacttc gtccaccgcc cctttgaagg cgtcatccgg
     7561 cagttggagc gccgctggcg ggaaaccgcg tccgccggcg tccgcagcga cctggcccgc
     7621 tacatcgacc tgcggccctg cgaaacctgc ggcggcgcgc gcctgaaaaa agaaagcctg
     7681 gccgtgaccg tgggcggcag gaacatttat gagctgtgcc tgatgtccat tcgcgaatgt
     7741 tttgagtttt tcgaagggct gaagctgacc ggtcaggaaa cgaaaattgt cgagcgcgtt
     7801 ttaaaggaga tcaaaagccg cctttccttt ctgctcaatg tcggcatgga ttatctgaat
     7861 ctggcgcgat cgacctccac gctgtccggc ggcgaatccc agcgcatccg gctggccacg
     7921 cagatcggct ccggactgat gggcgttctg tatgtgctcg acgaaccgac cgtcggcctg
     7981 caccagcggg acaacctgcg cctgatcgac acgctcaaga agctgcgcga catgggcaat
     8041 acggttctgg tggtggagca cgacgccgac atgatgctgg cctgcgacca tattatcgat
     8101 atggggccgg gcgccggcgc ccgggggggg gaagtgattt ttcagggcag tcccgccgaa
     8161 gtgctgcaaa gcgaaacatc gcttacgggg cagtacctct ccggccggaa atccatcggg
     8221 cttcccgcct ggcggaaggt caagggccgc cacatcgtcc tggaaggcgc aacggaaaac
     8281 aatttgaaaa atatcgacat tcgaatcccg accggcgttc tgaccgccgt caccggcgtt
     8341 tccggctccg gcaaaagcac catggtgatc gaaacgctct accaggtgat ggcgcgccgg
     8401 ctgaatcagc acagcggccc catggcaaaa atccgccgcg tccgggacat gggaggcatc
     8461 gagcgggtca tcatgatcaa tcagcagccg atcggacgga cgccgcggtc caatcccgtc
     8521 acctataccg gcattttttc ctttatccgc gatcttttca ccggccttcc ggacgcccgc
     8581 atccgcggct acaagccggg tcggttcagc ttcaacgtca agggcggccg ctgcgaagcc
     8641 tgcgagggaa acgggttgat caaaatcgaa atgcacttcc ttccggatgt ttacgtcacc
     8701 tgcgacgcct gccggggcaa gcgcttcaac gcggacacgc tggacatcaa atacaaggat
     8761 aaaagcatcg ccgatgttct ggatctgacc gtttctcagg cgctggattt ttttgccaac
     8821 atcccgtcca ttcgctcgca tctccaactg ctggcggatg tcggcctcgg atacatccgt
     8881 ctcggccagt cggccacaac gctttccggc ggcgaagcgc agcgcatcaa gctggcgcgg
     8941 gagctgggga aaagggtcaa ccagaacacc ctgtacattc tggacgagcc caccatcggt
     9001 ctgcactttg cggacattca gaagctgctg gacgtgctga tgcgcctggt ggacatgggc
     9061 aataccatgg tcgtcatcga acacaatctg gatattatca agtccgcgga tcacatcatc
     9121 gatctggggc ccgagggcgg tccgggcgga gggcggatca tcgcctccgg cacggtccgg
     9181 gaggtggcgc gaaatgaaca gtcttttacc ggcgcctttt tgaagaaagt tctatgaggg
     9241 gaggcacagg cacatgaatt ccattccatt ggttccttta aaataccggc attcgatcct
     9301 gctgattgac gacgaagaag ccgtcctgga aatcatgacc atggcgctgg ccgatgaagg
     9361 gtatgatctg cgcacggccg gctccgcgga agaagcgctg gccatcctga aagaatcgcc
     9421 ggatgtttcc ctgattattt ccgaccagct catgcccggc atgaccgggg tgcagttttt
     9481 tacgcaggcc cgccacatct gccccgacgc cctgcgtgtg ctgctgacgg gctacacgga
     9541 cacggacgcg atcatcgacg cgatcaacag cggggggatt caccggtatc tgaccaagcc
     9601 ttggcgcagg gaagaactgc tccacacgat cagagaaatg ctgggcaaag cggatctggt
     9661 gatggagaac cgtcggctgg acgaactggt caaaaaacag aatgccgagc tcatggaact
     9721 caacaaaagc ctggaagaaa aagtgcggct gaaaacaaaa cacctgaacc ggagcctgga
     9781 agagctccag gtcagctatg aaaaaacccg gatgattctg gaagggacgg tgctggccat
     9841 gtccagaatc gtggaaagcc gggatcccta cacggccggc catgaacagc aggtggccaa
     9901 aatcgcctgt gtcatttcgg aaaaaatgct gctgcccgcg gaccgggtgg acgccatccg
     9961 ggtcgcgggc cagcttcatg acatcggcaa aattgccgtt ccgtccgaga ttctgaccaa
    10021 gccggggcga ttgagccctc tggaaatgga gatggtcaaa acgcactgcc ggaacgctta
    10081 cgatattctc aaggcgattg aatttcccca tccggttgcg aaaatcattc tgcagcacca
    10141 cgaacggatg gacgggtccg gctacccgca gggattgaag ggggacgata tcctcctgga
    10201 agcgcggatc atcggcgtgg ccgatgtgat tgaagccatc tcctctcacc ggccgtaccg
    10261 ccccgccaag gacgttgacg tggcgatgca ggaaattacc gaccatcgcg gaacccttta
    10321 cgacgcgaac gtggtcgatg ccgccctcga cgtttaccgg acgaagggac ggcaagactt
    10381 cctgccgtaa aaccggccgc gattcgcggc aacaaaagtt cccaaccctc tatcggtaaa
    10441 gccggtttgc ttccatgtag cggatgacgc tgtccggcgt cagatagcgc agggagcggc
    10501 cctgctgcat tcttttccgg acctccgacg atgaaatatc cagaaaggtg gtctggcgga
    10561 aaaatatcga accgccgtgc ggctgctgaa aaacatcgcc cgccgcgtcg taaacgtagc
    10621 ggccggcaac gtccggcggc aggatttggt gaagcccccg gttttcatac cccgggcggg
    10681 tcatcaccac gaaatgacac agccgcagaa gatttttcca gtcatgccag gtggtgatgg
    10741 cgtcgaacgc atcctgcccg gtgatgaaaa aacattcgag acccgaatca ccggcattcc
    10801 ggaaatggcg cacggtgtcg atggaatagg attttcccgg ccgccggttt tccacatccg
    10861 acaaagaaaa gacatcgttt ccggcaacag ccagttgcag catttgcagg cggtgttcaa
    10921 aggagacgat cccgctcggg gtcttgtgcg gcggagtcgc ggcgggaatg aagacgatgc
    10981 ggtccaattt cagaagctcc gcgagctcct gggcggccct caggtgtccc aaatgaaccg
    11041 ggttgaatgt tccgccaaag aggccgagat tcatgtgcga acctgtccgt cgccgtaaat
    11101 gataaacttg gaggtcgtca gctcttccag gcccatcggc ccgtaggcgt gcagtttggt
    11161 ggtggaaatg ccgatttccg cgccgaggcc cagttcaaag ccgtcggaaa agcgggtgga
    11221 tgcgttgacc agcaccgtgg aggaattgac ctcgtttaaa aaccgctgcg cattcgcgta
    11281 gtctttcgtg agaatcgatt ccgtatgcag cgacccgtat ttttcgatgt gcgcgatggc
    11341 ttcgtcgatc gacgccacca cccggacggc caggatcagg tccaggtatt ccgcggtcca
    11401 gtcttcttcg gttgccgctt cgatcccttt cagaatcttt ccggtggctt cgcagccctt
    11461 cagccgcacg ccgtctttct gcagcctctc ggccaccttc ggcagaaaca cgggggcgat
    11521 atccgcgtgc accagcagcg tctccatggc gttgcagacg cccggacgct gcaccttggc
    11581 gttggcgcag atgttcacgg ccatgtccag gtcggccgag gcgtccacga aaatgtggca
    11641 gacgcccttg tagtgcttga tgacggggat tttggactgg gacacgacgg cgcggatcag
    11701 ctcctcgccg ccccgcggaa tgatcagatc aatctgctcg tcgagcgaaa gcaggatcga
    11761 aaccgcctcg cggtcggtaa aagggatgac ctgaatggca tggtccggaa gagggctttt
    11821 ctgcagcgcc tcctgcagaa cggacgcaat ggccaggttg gaatggatcg cttccgatcc
    11881 gccgcgcaga atgaccgcgt tgccggattt gaggcacaaa gcggaggcgt ccaccgtgac
    11941 attagggcgc gattcgtaaa tgatgccgat gacgcccagg ggaatccgca tccgccccac
    12001 cagcaaacca ttggggcgtc gccacatgga cgtcactttc ccgacggggt cgggcagagc
    12061 cgcgacttcc cgaagccccc gggccatggc tgccagagtg gcgtctttca cggtcagacg
    12121 gtcgatcatg gcggccgaaa gtcccttttc ccgcgcggcg gtcacatccc gctgattcgc
    12181 ggacaaaatc cggtcccggt tgcgaagaag ttcttcggcc atgcgcgtca ggacttcatt
    12241 tttctcgccg gttgggcagc gggaaagaag gcgcgaggcc gcgagcgcat cagcggctat
    12301 tttttttaca ctgtcagcaa tattcatggt gtccctcgat ttgaattccg gtaaaaggtc
    12361 ttgcaatttc tgtaaatatt gataaaagcc gcaacaactc ctgcaagaag ggagagcttt
    12421 gaataacagc catttttgtc atttcgatcg aagggagaaa tccatgtccc gaatgcaatg
    12481 agggatctta agatttctcg tcgctcacgc tcctcgaaat gacatttttc aaagcgctcg
    12541 aaggatcgtc ggagaaattt cgatcatgga cagaccctcc ggcggctcgc ggcatatgta
    12601 caaaacaaaa agacaggtgt caagattttt gcccgggtac ttttcatgag ccgattgcgc
    12661 atcaaggccg gaagaaaagc ttacgaaatc atcagagacg gcggctttca ttttgacgcg
    12721 gtttccgcct acttcggagc ggcggtcggg cctcgctggc tggtcgcgag cggtttcgat
    12781 ttgacgcttc tgaccggtga cttgctgggg cgctcccggc cggtgcatct catcggttcg
    12841 tccgccggcg cctggcgctt cgccgcctgg attcagccgc aggccgccga cagctaccgg
    12901 cggttcatga acgcttatat ttcggcccgc tttacaaggc aggatacccc cgagacaatt
    12961 ctggggaaat ttgcggatat tctgggttcg tatatcgaag acgacgcgct ttcctttgct
    13021 ctggccaaca aacgctaccg cctgtcggtg atcacggcac gatcgcgggg gcttgtcgca
    13081 tccgaaatgc ttcttctgca aaaaagcgct ctggctgcct gttttgcgct gaattttgtg
    13141 agccgcagcc aactgtatcg ctttgccgaa agggtggttt tctacaacgg ctccaagccg
    13201 ccccccttct gttttcaacc ctcctttcgg ggacggtatg tgcggctcaa cgaaatcaac
    13261 ttcaagcatg ccgtgatggc ctcgggcgcg attccgctgg tcgtcgcggg cgtgaagaat
    13321 attttcgggg cgccggatgg agtctatcgg gacggcggac tgatcgatta tcacttgtcg
    13381 caccagtttg ccggaaaaga aggcgacctg gtgctcttct tccatcacga agaaaggatt
    13441 attcccggtt ggctggacaa gaaaatcaaa cggcgcgcgc cggatgaaca gaccctggac
    13501 aacgtcgtga tggttttccc gtcgcagagt tttgtggacc gtctgccgga cggcaagatt
    13561 cccgaccgcg acgatttcct gacgcacatc gacaaccccg gccgtcggat cgaaaactgg
    13621 aacagggccg cttcgctttc cgccccgctg ggggaggaat tcctggatct ggtcgaaagc
    13681 ggccgcattc gggacatcgt ggaaaaaatg taggagggag tgttggggtc aggtcttgat
    13741 atatcagttt tcgcttggcg tcttaataac cttgctcacc gtcgtgtaat gaatccccag
    13801 atgatcagca atcgccttta gcgtataacc atgctggata tgcgctcgat gaattgcctt
    13861 atcccgttct gttttgtttc tttcttttaa aaacagagtc gataactttg gtcggcttaa
    13921 atatcgctgg attcgggaaa cctctttaac cgtctgttta tcctgcaata aatccctgtg
    13981 cgaatctaca aactcctctt ctcccaaaag aatctgccct cgtagctctt tccatggcga
    14041 tttcaatcca attcccgctg ttacaaattt ctgataaagt ttttccgcct cagtcttttt
    14101 ttgatgaaat aattctaaaa tccagcctgt tgttaaataa gccggcggag ttttcatccc
    14161 ggcggtaaaa cgatagctgc tccatcgcca ctgctccggt ttctccgtca ttcccgcacg
    14221 aaccggattt aaaacaacat accggcatag ctccagcaga taactttctt tttccaccag
    14281 gattgcctta taccggcctt ggaagacatg tccctccttt tgatgcatcc aattgtattt
    14341 ctgcgtataa accccattga gttgacgcat gcccttcgac agattcccat ccggcgtctc
    14401 aatcaggaga tggtaatgat tgtccatcag gcagaaggcg tgacacaacc agttaaatcg
    14461 acttacaacg ccggataata ttttcaagaa ggcttcccgg tcggaatccg aataaaagat
    14521 gtcattcttc gcatttcctc gcgtagttac atgatatacg gcatcgggat attctattct
    14581 aagaggtcgg gacatgctcg actcctacat cattaaacaa ttgaccggtt atcataaata
    14641 tactgatatt tcaagacctg accccagtga cgttgcccgc gcgaatttca cggataaaat
    14701 cgtcctcgtt gcgcacagga ttttcaaaaa ccgtcacaca ggagccgacc tgaaggatct
    14761 tgtgggcgtc gcttcctccc gtgcccggaa ttttcaactc ttccatcgcc cggcggactt
    14821 ttttcatggc cgactcaccg tggtccgggt ggcaaagttc gatcgcgtcc agcttcagtt
    14881 gatacacccg gtcccccgct ccgtagtaat gggatttccc gccgcgcgcc agtttgtagg
    14941 gatgcgcggc aatgacaatg ccgccttcct catgaaccgt gtggacgagc tcagcggctg
    15001 ggcattcttt tgtcaggctg cgcgaaaggc cgaaaaccag aaattcacct tcggagcggt
    15061 gggaggcgtc aaacgtgttg atttcctgtc cgttgatcag gagcatgccc cgttcggcgg
    15121 caatgtcccg aagcctcccg aattccgcat ccggccagcg catgccgtgt tcggtgaggg
    15181 cgaatccgga aagcccggcg cttgcggcct gtccgaccag atcctcgtat tcgataaagg
    15241 aacagccgga gtatctgcgg gtatgaatgt gaagatcaaa ggcaatcatc atcgggctac
    15301 ggcgtaaacg cagcgccaat tttgacgctg tcgtaacaag tccccattgc agttatttcc
    15361 actctctctg tcattcccgc gcaggcggga atccagacct cgttcctgcg cagcctgccc
    15421 tcgggaagtc gggggcgggg aaccagttcc cttattactg gatgccggcc ttcgccggca
    15481 tgacgaaaag gagcaaaagt gactttccgc gaagggatca atttttgttg gtccacttga
    15541 tctgaaactc aatttcttcg tttttcccct cccgttcatg ttccacatta aatgtggcgc
    15601 gggacggaac cgatattcgt tctccggcaa tcgtgatgat gaagcgcttg ccgttttcca
    15661 gcgcatcggc cagacggcga agcttggcgg cgaattcggc caccggatag gttttttcaa
    15721 gatcacggga tttctttttc atggcaagtc tcctttcgga tgaggattag ccttagaagg
    15781 agcagctgtc aagaagtagt gcgggtcttt aacccgcttt ggtggacggt atatggtgga
    15841 tggttgcagg gaacggtttc atccggcatt cgccggactt gcgactgttg cctgcctgga
    15901 cgaaggacgc ggtgaaaaat aaaagtctaa gtattcgaaa tagcatgaga tatgaaatct
    15961 tttcccttgc gggaacaata ttttatggta tttactaaaa aaaattgata ataggtcaag
    16021 aaattatcaa cgctttgttt ttgtttttat cattaaaata aacttggagg agggattact
    16081 gaagattaat caccagcaag accgcaggat attgtttgct tcattcaaaa cgtcttaaca
    16141 ccaaaatgag gagggaagtt aaaatggctg gagaagtatc gtatgcgtcg aaaccgtggt
    16201 taaaatctta cgacaagggt gttcctgaaa aaattcaata cgaagaaatc tgcatgcccg
    16261 acattctgga cagaagcgct gcccaaaacc cggatcgcat ggcgctgaat ttccagggct
    16321 acaccatgac ttacaaagag ttgaaagaca tggtggaccg gttcgccgcc taccttgccg
    16381 gcgtgggcat caaaaaaggc gatgccgtgg ccatcattct gcccaatatg attcccaccg
    16441 tcgtcgccta ttatgccatc catcgactgg gcggcattgt tgttttgaac aatcccactt
    16501 actcggaccg cgaactggag catcagctca acgattccgg cgccaagatc atgatcatca
    16561 tcgacctgct ggccaaccgg atgatcgatt taaggcccaa aaccaaggtg aagcagatta
    16621 tcatcacctc catcggggac tatctgcctt tcccggtcaa tcttctcttc ccgctggtcg
    16681 gcaagaaaaa aggcctgctg ccggcaaaac ccatcaaacc ggccaatgac gtgtttcgat
    16741 ggaaggactg catcgcgaaa tcgcagccca acccgcccaa agtaaaactg acgtttgacg
    16801 atacggccat gtaccagtac accggcggta cgaccggcgt ttccaagggc gtcatcctga
    16861 cgcacggcaa catcagcaag caggtgcagc agattcgcgc ctggttcccg aagtttgccg
    16921 gaacctacaa tgtcaacctg ggcgcgcttc ccttcttcca tgttttcggc atgtcctgcg
    16981 cgatgaattt ctccgttatc cagggatggg gcgatatcct gattcccaaa ccgcagccgg
    17041 agcctcttct ggaagcgatt cgcaagttca ggcccaactt cgccgcgctg gttccgacca
    17101 tgtttatcgg tatgctgaat catccaaaaa tgaagaccac cgatatgacc tgcttcaaag
    17161 gcttcttttc cggcagcgcc ccgctgccgg tggaagtgat tcatgatttt gaaaaaacgg
    17221 caggatcggt catctgtgaa gggtttggca tgacggaaac cacgccggtg acccatacca
    17281 atccattcga aggcatccgg aaaatcggtt cgatcggcct ccccgtatcg gataccgaat
    17341 gccgcatcgt cgatctggat aaaggcgaaa cggatatgcc cgtcggcgaa ccgggcgaac
    17401 tcattatccg cggcccgcag gtgaccaaag gctacctcaa caaaccggaa gaaaccgccg
    17461 gcctgctcag agacggatgg tgctatacgg gcgacatcgc cacaatggac caggacggct
    17521 acttctacat cgtggaccgc aagaaggata tgattatcag cggcggattc aacgtctatc
    17581 cccgtgatat cgatgaagcg tactacgagc atccgaaggt tatggaagcc tgcgccatcg
    17641 gcattccgga tgcaaaacgg ggagaaaacg tcaagctgtt catcgtactg aaagaagggg
    17701 aaacggcgac ggccgaagag atgattgaat acggcaagtc gaaactggcc acctacaagc
    17761 tgcccacgga agtcgaattc cgcaaggaac tgccgaaatc gaccgtcggc aaggttctgc
    17821 gcaaggaact gagggcggaa gaactggcca agcgcaagaa ataggcgtct gttcctgaaa
    17881 attgatgaac tggtaaaaag tcgatttcgg caccttttcg tcataccggc gaaggccggt
    17941 atccagtagg tacagtgggt tatggacccc ggccttcgcc ggggtgacgg ctcgaattac
    18001 tttttacgag accgtcaaaa ttaacaaaaa aaggcttgcc ccggcggggc aagccttttt
    18061 ttacagtcgg ctgttgttta ttttacggag actaccggac aggaggactg caggatgata
    18121 tactgggcat tggacccgaa cagaagcttg ccgactttgg attttttctc tatgccgatg
    18181 atgatctcat ccactttctg gtcttccgcg aattccacga tatcttcccc gggggtgagg
    18241 ccccgcacca gaatatgggt ttcgcattct atcccttcgg catccatcgt ttccttgaca
    18301 taggcgaggt gcttttcaac tttgtccagg tccctgcgct ccctctccga tacccgctcc
    18361 atggagctcg cgacatacag ggaagcgtta aaagctttgg cgtgtttttt cgccacttcg
    18421 agcaccgccc gatcgacgtc caatcctccg acatatgcga ctaaaatttt cattattccc
    18481 cccccgtcct tcccgttgag tatggatttt gtgaacacat tctctgattt ttgtttggac
    18541 ggagtataag aaaaacagtt tataatgtca atctgtttct taagcgggag gcctgtttta
    18601 cgcgcgggga gcggatgttt tgtcccgggg tttcggcgga cgagccgcga gcgccaggat
    18661 acagcagaca aagatcaatg ccgtcgtgac ggcgaagggc agggtatagc tgccggtcga
    18721 atcgtagaga aatccggcca gcagggggct tgcggcggaa gccagcgtga cgatcggtgt
    18781 ggtccagccg acgatccggg aaaaattcgc gcgtccgaag taggcgccca tcgtgtgcgg
    18841 caggaggacg atcattccgc cgaagccgag gccggtcagg atggaataaa ggtaaacgaa
    18901 aacgatttcc cgggcgttca tgagggacag aagcccgaag cccagacagg ccatgaaaac
    18961 ggcggccaga tgccttcctg aaaaccgcat tcccaggatt ccgctcaaga gcctgcccag
    19021 gatgctcatg cccagcatca gccccagagc cgtggcggaa accatcggcg aatgtttcaa
    19081 atcctgcaga taagcgacct ggtgcgtggt cagcagggtg gtggcaaaca gtaaaacgga
    19141 aaacagcgcc agcagaatcc acagcgccgg cgtccgcagg gcgtcgcgca cgctccaatc
    19201 ctcccgggtg tgatagacat ggccggtcgc tttttcccgc cactcgtcaa actgttcctt
    19261 tgaggcgtgt ccgtccggaa actggctttc ctcctccggc atattgcgga tgagtaaacc
    19321 ggctccggca acgcccatga tcaggtgaag acaggccagc gccgcccagg caaagcgcca
    19381 gtccagtccg gaaatcagca tgctgatgag cgggggcatc agaaagccgc cgatgcctcc
    19441 ggaagcgaaa agaaaaccaa gcgccagaga ccttttgcgg ataaaccagt ggttgatgac
    19501 ggtggtggcc gacaggaact cggcgaaagc gagcccgagg cctcccatca ctccgaaaaa
    19561 gagataaacc tgccagattt cgcgaacctg ggacatgccc agcagaccca gcgccgcaat
    19621 gacattgccc agcacaatgt ttttccgggc tccgaaacgg gcgacggtaa cgcccgccag
    19681 cggccccagc atgccgccga tgatgagaaa cagcgtatag gggccggaca gggccgagcg
    19741 gctccatccg aaagattcgc ccattgacgg cagaaagacg ccgaaggcgt acgtgacatt
    19801 cccgcagatg gccccgtagg ccagcatgac gcccgccagt gttgtccagc ccgaaaaagc
    19861 cggctgcgtt gatggtgatg aatggatcat gcagatccct gcttgcctgc ctttatgaaa
    19921 ataactatag gaagaacgcc cgcgtctgtc aataaataag cataagctct tgatattgct
    19981 catattattt tgaattccca aagaaggaga aaaaggttac aatgcgttca cggcggatct
    20041 gcccttgcaa ggcttgccgt catcaggggt aaaagattat gacaggatcg gatgttctgg
    20101 tagtggttgg ccacagcggc gttgtgattc cgccggagat ttctctggag gacttgacgg
    20161 acgagtttac cgctttgctc aaaaacgtgg actggtacac gcagtggctg tatgactttc
    20221 gtgatattct gggaaaccgt cagttggttt ttccctgctg cagcattctg ctggatgcca
    20281 accgcgatcc cgccgatctg gacgaagccg tgccggttcg ggacgttttc ggccgcccga
    20341 tttaccgcag cgcctatgag ccttcgccgt ccatgcgggc ggcctggtcg gacaagtatc
    20401 tgaaaccttt tcaccgcggc atcgaagaaa atatttccgc gggcgccggc cttctctttg
    20461 acggccattc aaccgtcacc gcccgcggcg tggccgccaa ccagatcgat ctgatgaatt
    20521 ttcagcatac ggaccgcgag gaaaaggcgc tgtactactg tccggacgtc atcgtggaaa
    20581 catacgccga ggaactgcgc aagcggctgc ccgacgcgct ggtgacggtg aacgcttcgg
    20641 aatatgtggc ggtgcacggt catatctgcg ccgcgcactc ggtcaatgcc cttaaaaggg
    20701 tcggcgcgcg tgctccggcc ttcattcagg aaaccaacga aaacctctat aagaacagcg
    20761 acgggacgcc gaatgtcggc cagatcaacc ggctgaggcg cgcgtttgcc gaatcgctcg
    20821 cgcagacgct tcagtcgctt caggaatcac agaaagtcac gatgatcgat ctgcacctgg
    20881 gcaagcaggt ttacgactat gactgcggcg tgcaggcgct tcagacggtg atgacgtatt
    20941 acggcgtg
//
LOCUS       DAOSRM010000030        20733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2761_length_20733_cov_94.034868, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000030 DAOSRM010000000
VERSION     DAOSRM010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 20733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20733
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2761_length_20733_cov_94.034868"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PLA58_04570"
     CDS             complement(<1..562)
                     /locus_tag="PLA58_04570"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR62101.1"
                     /translation="MGNRRQTKETSVDDIRRQTVRLKKRKPSDREPKPVENELKESEE
                     RLKSVVSRSPIPAFVISKDHRILYWNKALEQLTKIRAEDVIGTSQQWRAFYARKRPCM
                     ADLLVSRAQEKISKWYTGKYSKSKLLDEAYEAIDFFPELGHRGKWLRFTAAIIRNEAG
                     DLIGAIETLEDITRRKKAKEALLKAHR"
     gene            826..3885
                     /locus_tag="PLA58_04575"
     CDS             826..3885
                     /locus_tag="PLA58_04575"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOR62102.1"
                     /translation="MQKNPLNPDPTGFSKDGRLAVKIFQEVPLMRLLKKVLIAVLVII
                     LLIGLAGFLILPAVLKPVLTKKISETLHREATIDEIKINPFDLSATVRGFKLADPGGH
                     ASFAAFQELLVNVDVLTSIFRRALILEEIRLNQPYIGITRKADGTYNFSDLIPKEETQ
                     KKEPSKPFLFSLNNVQISNGSADFHDTPNKTNHSIRNLNLDVPFVSNIDHYMKHYVEP
                     RFSAVVNGHAVSVTGKTQPFQASRESLLDIDIRNIDVPFYLQYVPVKMNFKLTQALLD
                     IRLQVHFIMPEKQAPELKLTGRADLKKVALDDLRGSKILRLPVLSVNLASVQPLVPKV
                     HLSEVSLGGLQLAVRRSPRGEINLLHLAGTQAKAKPKETAAEKKKPDKAETKKKELDL
                     QVDNILLDGADISFTDEAPVRPFRTRIHPLRLAVSNFSLKKGNKAGIDWTLVMDQKSR
                     ITAKGPVCLSPLGADLALDVKNLALRPFQSYVPDTIAMDITSGDISTAGKFILATDAQ
                     DKPTVKYSGNLSVDRLATIDRARANDFLKWKQLRFESLAAGTNPLFVNIKEISLRDFF
                     AKIVIHPGGVTNIQHIFGEAKKETPPPDAAGKTADEKTAPAKTPQAAPDAKPAPPPPD
                     IKIGRVSFHGGTVDFADRNIRPNYAVTMLNLRGSVTGLSSQQISRAKVDLKGNLGYGS
                     PLEIAGTVNPLAKDLFADIKLSFKDIEMSPVTPYTSKYLGYPITKGKLNFNVAYLVDQ
                     RKLAAENKIFFDQLTFGDKVESPEAIKAPVPLAVSLLTDRNGQINLDIPLSGSLDDPK
                     FRIWPIVWQVLVNIITKAVTAPFSLLSSLTGGGEEMSYVEFNYGSADLPEAGLKKIGA
                     LSKALYDRPQLKLEIEGYVDATQDSEALKKAKFNRLLKTQKRKEMIDRGETPSPLEEM
                     AIGQAEYEKYLTLAYKAAKFSKPRTALGAAKSLPPKEMEKLLYDNTAVTDGDLEQLAA
                     RRAQAAREQLLKDGKVEAGRVFIVQSKTKTPAKKDKIKDSRVDFKVK"
     gene            3923..5068
                     /locus_tag="PLA58_04580"
     CDS             3923..5068
                     /locus_tag="PLA58_04580"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HOR62103.1"
                     /translation="MKKFIAIALLLVCLWASSCAPLRSGIVPTGEISNRCLPSFPDQD
                     GWYGGDGAYSIALDGRRSLWLFGDTFVSEEKGRKDRIGMEVIPGTTLAVSTCKGEQFS
                     IRYYLKKANGKFASSFGGDQWLWPQDPFIADRILYIPFLVIRALPGPPRPFHFEIAGH
                     RIARIKDYSAEDPHDWPVDYLDWTGAIAPGIEALAAASVVHDRFVYFYPLYRSGKDGV
                     PIHGNLPARLPVNRLENPAGHFEYWTSDGWQKEVTPKNARMIFSPGLSELSVRYLAED
                     GEWLAVYLSPENKGDRLLFATSRQPEGPWSPPAVLIPSIDEVDPKSPLYHPQTFCYAG
                     KEHRQFARGRELVATYVCNSMEDIDDQDSFLRKNLFLYRPQVKTIRR"
     gene            complement(5078..5425)
                     /locus_tag="PLA58_04585"
     CDS             complement(5078..5425)
                     /locus_tag="PLA58_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOR62104.1"
                     /translation="MEFNLIKTHAQSGYEILKDIKFSWPIARMVLEHHERIDGSGYPN
                     GLTGNNILPESRILAVADVVEAMATHRPYRPALGLEPALQEITQNRGVLFDEEAVDAC
                     LRLFREKGYTIKD"
     gene            complement(5431..5853)
                     /locus_tag="PLA58_04590"
     CDS             complement(5431..5853)
                     /locus_tag="PLA58_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR62105.1"
                     /translation="MIADDSRVNLYFLKSILEAQGWLVREAENGKAALEMARASRPDL
                     IVSDILMPVMDGYSFYRECKKDEILRNIPFVFYTATYTEPKDEKFALDLGADRFILKP
                     EEPEVLIKILVDLLEEKERISLAASKPFRPRFFARRED"
     gene            complement(5872..7611)
                     /locus_tag="PLA58_04595"
     CDS             complement(5872..7611)
                     /locus_tag="PLA58_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOR62106.1"
                     /translation="MFKCHRKNAEGKAVIFFAHLMRWLFILTLVSFGCSSMALAESRV
                     VKIGVYENSPKVFTDESGRPSGIFVDIIKYIASKEDWELQYVAGTWAEGLERLEKGQI
                     DLMPDVALTAEREKKFSFHKIQVLSSWFQAYARKGSGLQSILDLNGKRVAILDQSVQQ
                     EAFTRFSRGFGLDTKLVAVADYKTMFEMVAKGEVDAAVANQFYGSMHARKYDLVDTAI
                     VFEPSALFFAAPKNQSQELLDAIDKHLDFLKKNTKSAYYATLKKWTSEEVRFKLPVWL
                     QILGLITGIVLLMSLGGSVILKYQVNARTRELQKINAEMEGRIIERTAELALAMEKAQ
                     SADRLKSAFLATMSHELRTPLNSIIGFTGILLQRLGGPLNDEQDKQLKMVYNSAKHLL
                     SLINDVLDISKIEAEQLNVVSETFSLRETVEKVIKTSQPLAENKNLKLTAEIAPEIGL
                     MKSDRRRVEQILLNLLSNAVKFTEQGFVRMKCEIQNEKIVISVADSGIGIKPEDIGVL
                     FNPFRQIESGTTRKYDGTGLGLSICRKLARLLGGEVRVESVWGQGSTFSLMLPQEGEV
                     EKEQHLPKQEREK"
     gene            complement(7623..8174)
                     /locus_tag="PLA58_04600"
     CDS             complement(7623..8174)
                     /locus_tag="PLA58_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62107.1"
                     /translation="MNFSYNFNEKYRAVSYVFIQADDEMSNYDYTEWGIGLQYQTPVA
                     WLSFLAYYQQGYSKDEDRHWALEQKPSINLNLSKTFFNFKVFNQIRYEYRITPDWNDA
                     RIKNTLNISLPDLLLQPYVGWELFYENRDKAVMLNRIKAGINKNIGAHVSVGPYFRMD
                     FSNVNSRWEWTRQLVGLQMTINY"
     gene            complement(8412..8816)
                     /locus_tag="PLA58_04605"
     CDS             complement(8412..8816)
                     /locus_tag="PLA58_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR62108.1"
                     /translation="MKGNILVIEDNEQNLYLVRFILEKNGYTVHAAMDGQTGIDRAAE
                     LKPDLILLDIQLPVMDGYTVAKHLRADPQVSGIPIVAVTSHAMVGDREKAMEVGCNGY
                     IEKPINPDTFMSEVELHLPKREQSTEKKALKD"
     gene            complement(8813..10825)
                     /locus_tag="PLA58_04610"
     CDS             complement(8813..10825)
                     /locus_tag="PLA58_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR62109.1"
                     /translation="MTKDSLSVPRNFVRTFPSRLIILFLTILFVCCASQIFSAGISCA
                     AAPPDTQGANGQNSKKHLLVITSQPYLTDWFDTLNRAMRKTLFPALSSDSKISYEYIG
                     SESLTDEDYDVKLMDFLREKYANIKIDMVLAFMPVSCQFILDHGESLFPGIPVVFVLP
                     AKKQLAVISGRPQSGLVRSGANAIPDTIERIRVLLPETQRLVVVCGSGADDLNYQKVA
                     QEALAGKGWPKTVEYLQGLPAGELAATLERLPEASAVLMLVYLQDRDGKPLTTVQVMK
                     AVAPRSKAPIFSFYDTVLGLGIVGGKLSSAEAYGEASAQTALKLLRAGPDAPLTETVA
                     EARDIYDWRQIEKWKISPERLPDRSDIRYRQVTFWEENRGKIIAVAAIILLQAVLILA
                     LLFNLSRRKRAEAALRDSEKTSRSRLEEQVRERTAELEVAKERAESADHLKSAFLATM
                     SHELRTPLNSIIGFTGILLQRLGGPLNEEQDKQLSMVYNSARHLLNLINDVLDLSKIE
                     AEQLNVAHESFNLRESLENVLKTSQPLADSKGISLSADIAPDIGTIISDRRRVEQILL
                     NLLSNAVKFTEQGGVRLRGFVKDRKIVLVVSDSGIGIKPEDMGKLFNAFRQIESGLTR
                     KYDGTGLGLSICKKLAHLLGGEIEAESVWGQGSTFTFSLPQEGDKA"
     gene            complement(10925..11857)
                     /locus_tag="PLA58_04615"
     CDS             complement(10925..11857)
                     /locus_tag="PLA58_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR62110.1"
                     /translation="MSFVRPDIIRPPSEHASYYLPLTSGCSNNSCAFCAFSFTTLGIR
                     DLDDVKREIDAMSLYAKSRMWMAGQPDIVYAILRQWDGKRVFLQDGDALVYPYPRLME
                     ALQYLNGKFPALERIASYATPQDVLRRSVQELRDLKKQKLGILYMGVESGDDDVLKKI
                     RKNVSHDQMVEAAKKVKASGILLSVTVILGLGGVAGSEKHALETARILTEMDPDYAGA
                     LTLTLIPETEIYKEWESGRFEMITPFDSLRELKTMVEHSTFSNCFFSSMHASNYFSIR
                     GSMPKDKGKILRQLQALLSRRDPNMLRPEFMRGL"
     gene            12060..13484
                     /locus_tag="PLA58_04620"
     CDS             12060..13484
                     /locus_tag="PLA58_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR62111.1"
                     /translation="MSLDPLFYPKSVAVIGASDNLGGGKLPYFQILQANGFTGALYPV
                     NPRRVEVSGVKTYASIDDLPEPVDFAIVAAPVEQSVDIVKAAVRKQIKFIHFFTSGFG
                     ETGNLQLEQEIIREARKGHLRIVGPNCIGVQCFESKVSFRLTNLQEGPGSIAFLGQSG
                     GITSSFVAMATTRDIRLNKVVSYGNQIDLRLEDYLEYLADDDKISLIACYVEDIKDVN
                     KFLKALKRTTAKKPVIILKGGKTELGSKAAASHTGAMASNYTIWAAAVRQHGGLLVDN
                     FEELMNLAMLGTAQRFPRGRRVGFMGAGGGISVLFSDLAAASGVLLPELSPRTQRMIS
                     EKIMGVNTSTTNPVDLGAFGFDFNVMLHTMKALNEDDNIDVIVPYFSVEYIMHAELFL
                     KVTNSAHSIMEMARQIKKPVIPVLSCFLENDLEAERVRISTFNALRKAGFPVYPTIQE
                     ALYVIETYFEWAEKRPRRGEKNAA"
     gene            13573..14400
                     /locus_tag="PLA58_04625"
     CDS             13573..14400
                     /locus_tag="PLA58_04625"
                     /inference="COORDINATES: protein motif:HMM:NF002614.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HOR62112.1"
                     /translation="MKKIMICLAVALCFLSCGWVCAAENPGQPVPVGSAFAMPAHSPS
                     YPPGPYRFIDREFLIITYETDMEALRKQVPEPLEIPEALVKFEFIKMPDSSGFGSYTE
                     AGQVVPVIFKGQKGQYVLSMFLDDEAPIVAGREIWGFPKKLAYPSLGVDPVGRDTLVG
                     RLKYGSVEVAVGTMGFKYAPVDGQAIKKSLEDVPNYLLKIIPGPDGSTVVRQLVQYNL
                     KDVTVKGAWTGPAALQLFHHALAPVASLPVKRIVSCVHFTSDLTLPYGKVVHDYLKK"
     gene            14432..15778
                     /locus_tag="PLA58_04630"
     CDS             14432..15778
                     /locus_tag="PLA58_04630"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOR62113.1"
                     /translation="MNRPSLFHPAPPLAALAIVLIFLFSGVVLAAAQEIAPAAETAFP
                     TRDYLTGNWSGVRDRLHHSGVRVGLNYSTETMFNVAGGEKQGGTYADNIDLALLFDLA
                     KLTGIPRTTFLVKMSQRDGDSVSARFIAPSQDGNTFPVQELYGGQTFKLVNVQFNTRL
                     ADDRIDLAYGRLVANDDFLRSDLYCQFLNNSFCGSPKPVFLQNPFTFTAYPLATWGAR
                     AALDTPSRNWTFQAALYDGDPEGRRGDPAGENQNLHGTWWKFGNNGVTLAGEIRYHVN
                     RGSDSALPGVYKIGGFYMTGRFLDVSRMGNVTTTGDAMIWLLADQMLYRESPGERRGL
                     SAFGSLVLSLTDKTNPMTSYFNAGLVYEGLFCTRPRDRTGLAVTAGFFGKDYKDGLRA
                     AGLETKSHEAVIEFNHLFLVGRGIGIQPDIQYIIRPAGTGAISNALAVGVKVSVDF"
     gene            15802..17193
                     /locus_tag="PLA58_04635"
     CDS             15802..17193
                     /locus_tag="PLA58_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOR62114.1"
                     /translation="MRSGYKTTVFYICGTAALGGLLFGLDQGFIANSLDTIRKVYGLD
                     IRSAEHYSSILATGGVIGALLSGLLARYLGRKRSLVLSGFIFCASSLVSALLPPLAVM
                     YVCRFLLGFAIGIASFIVPLYLSETAPASIRGAMGTLFQLLITIGIFLISLTNFIIAQ
                     WISSPETALPLMFSVIVVFAGLMFAGSLFLPESPRWLMLKGRKDEATAVLQKTLNTPE
                     DVLREIHDMEATLGRRGFRIGNLLKGYFIKILLVGIFLQVFQQLVGINVMIYYAPTIF
                     GYAGLAGMIAMMTVPTVNMLFTFPAIRLVEKWGRKKLLYVGALCMMISMTAAGGAFAY
                     IGNPSDPAAIGLAPKGILLAAVIFYIFGFAVSWGPVAWLVCSEIFPIRGREVGMTVTT
                     MVNWTFAGLLMDNALTFMEAHGHASIFFLFAFFCILAIAFVAAFVPETRGVTLEQIEG
                     NLKAGKKMRDLGD"
     gene            17197..18207
                     /locus_tag="PLA58_04640"
     CDS             17197..18207
                     /locus_tag="PLA58_04640"
                     /inference="COORDINATES: protein motif:HMM:NF005481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOR62115.1"
                     /translation="MKTVSYRSLGLSNTGEMFQKAFTEKYAVPAYNFNNMEQLQAIIA
                     ACVQTASPVILQVSGGARKYADQTLLRYMAQGAVAFARRMGGTIPIALHLDHGDSFEL
                     AKSCIDSGFSSVMIDGSHFPFEDNIALSKRVVEYAHPRNVSVEAELGVLAGIEDEVSH
                     AQSHYTNPEDVEEFSGRTGVDSLAISIGTSHGAFKFKPQDCTLNADGVLVPPSLRFDI
                     LEEIEKRIPGFPIVLHGASSVVPKYVAMINQYGGRLEAAVGIPEEQLRRAAESAVCKI
                     NIDSDGRLAMTAAIRKVFAENPGEFDPRKYLGPARAELIELIKHKNINVLGSAGRYPA
                     LP"
     gene            complement(18342..18737)
                     /locus_tag="PLA58_04645"
     CDS             complement(18342..18737)
                     /locus_tag="PLA58_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOR62116.1"
                     /translation="MNHVHHVHLFASDIEKSLAFYQNAFGGKIILDENMAGARNVFMR
                     VGTGRIHFYDQAPSFSGRGSIHHFGIQTDDIEGVVEKLKAMGVPFKKGVTDIGVWKYV
                     MVPAPDHVLIELFQVNRDALPEAYRSYFD"
     gene            complement(18846..19751)
                     /locus_tag="PLA58_04650"
     CDS             complement(18846..19751)
                     /locus_tag="PLA58_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOR62117.1"
                     /translation="MLTRRKITDVVETMSGIPEKIIDFHVHLFPDRLFDAIWEAFLKQ
                     YNWHIIHRCYYRECIERLRRKGVSVIVYSNYAHRAGIAEELNRWNEKVLQEYEDLYCF
                     AAYHPGDDHALDMARRLVENPKILGFKLQLLVQNFYPFDERLFGLYDLVMKKNKRILF
                     HTGTGPVGNPYVGLEPFMKVLDRFPDLPATIAHLGAHEYAGFMELVDRHPQLYLDTAF
                     CFLPAPYDGYNLGPGYLEKYKSRILYGSDYPNLITPWEAEIENLLKMNLSPEFYDRIF
                     YDNAAALIRSLTAQGETGESGLTSG"
     gene            19912..20250
                     /locus_tag="PLA58_04655"
     CDS             19912..20250
                     /locus_tag="PLA58_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62118.1"
                     /translation="MKRKWAVLLVVMAIAAGAGPARAEITQSDLVVLDVALIRPVGIV
                     SVVVGTALFILSLPVSIPSGSVGTAANSLVKDSFEYTFIRPVGDFDYELGTWGKEAPP
                     SSESSGAAAK"
     gene            20291..20566
                     /locus_tag="PLA58_04660"
     CDS             20291..20566
                     /locus_tag="PLA58_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62119.1"
                     /translation="METLLDTQLAVPLSQVALLLGLSTLILLFGRVKLALIINYCFTL
                     YWGFFLNPSFRSDLGELMLNTYTYVYIGVGLIIVVLALIGFLSSKDR"
BASE COUNT         4663 a         5933 c         5460 g         4677 t
ORIGIN      
        1 cccggtgcgc cttgagcagc gcctccttgg ccttcttgcg ccgggtgatg tcctccagcg
       61 tttcgatggc gccgatcaga tcgccggctt cgttgcggat aatcgccgcc gtaaagcgca
      121 gccatttgcc ccgatggccg agctccggga aaaaatcgat cgcttcgtag gcctcgtcca
      181 gcagtttgga tttggaatat ttgccggtgt accacttgga tattttttcc tgggcgcggc
      241 tcaccagcag atccgccatg caggggcgct tcctcgcgta aaatgcgcgc cattgctggg
      301 aggtgccgat gacgtcttcg gccctgattt tggtaagctg ctcgagggcc ttgttccagt
      361 aaaggatgcg gtgatccttg ctgatcacaa acgcgggaat cggggaacgg ctgacgacgc
      421 tcttgaggcg ctcttcactt tctttgagtt cgttctcgac gggtttcggt tcacgatccg
      481 acggtttgcg ctttttgagg cggacggttt gtcgccgaat gtcatcaacc gaagtctctt
      541 tcgtctgccg cctgttgccc atgacttcac ccttactcaa agttgaggaa cggcctgaat
      601 ccgccccggg ccgccaaacc cccgcgccgc cgctgaagaa tggatctgaa aaaaattgcg
      661 ttggaaatga ttatattggc tttggcaaag aagtcaaacg aatctttaaa ataacggagc
      721 aaggttcgga ggttcaataa tggacggccc cctgtctgat ttctacgcct gcggatgggg
      781 ccctcacaga ggccccacgg attgtcaggc gctgcagaat gtggtatgca aaaaaatccc
      841 ttgaaccctg atcccacggg attcagcaaa gacggccggc tcgccgtcaa aatctttcag
      901 gaggtgcccc tcatgcgtct tttgaaaaaa gtcctgatcg ccgtcctcgt tatcattttg
      961 ttgatcggcc ttgccggttt cctgattctt cccgccgttc tcaagccggt tctgaccaag
     1021 aagatttccg agaccctgca ccgggaagca accatagatg aaatcaaaat caatcctttt
     1081 gatctttcgg cgaccgtccg gggattcaaa ctggccgatc ccggcggcca tgcctcgttt
     1141 gcggcctttc aggagctgct cgtcaacgtg gatgttctga cctccatctt tcggcgggcg
     1201 ctcattctgg aggagatcag gctgaatcaa ccctacatcg gcatcacccg gaaagcggac
     1261 ggaacctata atttttccga cctgattccc aaagaagaaa cacaaaagaa agagccttcc
     1321 aagccgtttc tcttttcact caacaacgtt caaatttcca acggttcggc ggattttcac
     1381 gacaccccca acaaaaccaa tcactcgatc cggaacctga atctcgatgt tccgtttgtc
     1441 tccaatatcg accactacat gaaacattat gtcgagccca gattctccgc cgtcgtcaac
     1501 ggccatgccg tttcggtcac cgggaagacc cagccctttc aggcatcgag ggaaagcctg
     1561 ctggacatcg acattcggaa tatcgatgtt cccttttacc tgcagtacgt gccggtgaaa
     1621 atgaatttca aactgactca ggcgctgctg gacatccgcc tgcaggtgca tttcatcatg
     1681 cctgaaaaac aagcccccga attgaagctg accggccggg ccgatctgaa gaaagtggcg
     1741 ctggatgacc tgcgcggaag caaaattctg cggcttcccg ttctgtcggt caacctggct
     1801 tccgtccagc cgctggttcc caaggttcat ctttcagagg tttcgctggg cggactccag
     1861 ctggccgtcc ggcgcagccc gagaggcgag atcaacctgc tccatctggc cgggacgcag
     1921 gcgaaagcga aaccaaaaga aacggcggcg gaaaagaaaa aacccgacaa ggccgagaca
     1981 aagaaaaaag agctggatct tcaggtggac aatattctgc tcgatggagc cgatatttcg
     2041 tttacggacg aggcgccggt ccggccgttc cgaacgcgga ttcacccctt gcgcctggcc
     2101 gtctcgaatt tttccctgaa aaagggcaac aaggccggca tcgattggac gctggtcatg
     2161 gaccagaaaa gcaggatcac cgcgaaaggg ccggtttgcc tctcgccgct tggcgccgat
     2221 ctggcgctgg acgtgaaaaa tctggcgctt cgtcccttcc agtcctatgt cccggacacc
     2281 attgccatgg acatcacaag cggcgatatt tccacagccg gaaaattcat cctggcgacg
     2341 gatgcgcagg acaaacccac cgtgaaatat tcggggaatc tttccgtgga tcgtctcgcg
     2401 acgatcgacc gggcgcgcgc caacgatttt ctaaaatgga agcagctccg ttttgagtct
     2461 ctcgccgccg gaaccaatcc tctctttgtg aacatcaaag aaatttcact tcgcgatttc
     2521 tttgccaaaa tcgtcatcca cccgggcggc gtcaccaata tccagcacat cttcggcgag
     2581 gcaaaaaagg aaacgccgcc gcccgacgcc gcagggaaaa cggcggatga aaagaccgca
     2641 cccgccaaaa ccccgcaggc cgcgcctgac gcaaaaccgg ccccgccccc gccggacatt
     2701 aagatcggca gagtcagctt tcacgggggc accgtggatt ttgccgaccg caacatccgg
     2761 ccgaattacg cggtgaccat gctcaatctc cggggaagcg tcacgggcct ttcctcgcag
     2821 cagatctcgc gcgcgaaagt ggacttgaag ggaaatctgg gctacggatc gccgctggaa
     2881 atcgcgggta cggtcaaccc gctggccaaa gatctctttg ccgatattaa actcagcttc
     2941 aaagatattg aaatgagccc ggtcacacct tacaccagca aatatctggg ctatccgatc
     3001 accaaaggca agctcaattt caacgtcgcc tacctcgtcg atcaaagaaa actggccgcc
     3061 gaaaacaaaa tctttttcga ccagctgaca ttcggcgaca aggtggaaag ccccgaggcc
     3121 atcaaagcgc cggtgccgct ggccgtttcg ctgttgacgg accgcaacgg gcagatcaac
     3181 ctcgacattc ccctgtccgg aagcctcgac gatccgaagt tcagaatctg gccgattgtc
     3241 tggcaggtgc tcgtcaacat cattaccaaa gccgtcaccg cccccttttc ccttttgtct
     3301 tcgctgaccg gagggggaga ggaaatgagc tacgtcgaat tcaactacgg cagcgcggat
     3361 cttccggagg cggggctgaa aaaaatcggc gcgctttcca aagcgctgta cgaccgcccg
     3421 caattgaagc tggagattga aggctacgtt gacgcaacac aggattcgga ggccctgaaa
     3481 aaagcgaaat tcaaccgttt gctcaaaacg cagaaacgca aggaaatgat cgaccgcggc
     3541 gaaaccccct cgccgctgga ggaaatggca atcgggcagg ccgaatacga gaaatatctg
     3601 acgctggctt acaaggcggc aaaattttcc aagccgcgca cagcgctggg cgccgccaaa
     3661 agtctgccgc ccaaagaaat ggaaaagctg ctctacgaca acacggccgt gacggacggc
     3721 gatctggagc agctcgccgc cagacgggct caggcggctc gtgaacagct tctgaaagac
     3781 ggcaaggtgg aagcggggcg cgttttcatc gtccagtcca aaaccaaaac gcccgcgaag
     3841 aaagacaaga tcaaagacag ccgcgtggac tttaaagtga aataacggcg ctgcgcattt
     3901 cgctcccggg aaaacggcga tcatgaaaaa atttatcgcg atagccctcc tgcttgtgtg
     3961 cctttgggcg tcctcctgcg cgccccttcg gtcgggtatc gtccccaccg gggaaatttc
     4021 aaaccgatgt ctgccctctt tccccgatca ggacggctgg tacggcggcg acggcgccta
     4081 ctccattgcc ctggacgggc ggcgatccct gtggctgttc ggagacacct tcgtctccga
     4141 agaaaaggga cgcaaagacc ggatcggcat ggaagtgatc ccgggaacga cactggccgt
     4201 ctccacctgc aaaggcgagc aattctccat ccggtattat ctgaaaaaag caaacggcaa
     4261 attcgcatcg tccttcggcg gggatcaatg gctctggccg caggacccgt ttatcgccga
     4321 ccggatttta tacataccgt ttttggtcat ccgggcgctt cccggaccgc caagaccctt
     4381 ccacttcgaa attgccggcc acaggatcgc ccggatcaag gattattcgg cggaagatcc
     4441 ccatgactgg ccggtggact acctggactg gaccggcgcg atcgctcccg gcatcgaggc
     4501 actggccgcc gcatccgtcg ttcatgaccg ttttgtctat ttttatcccc tgtaccggtc
     4561 cggaaaggac ggcgttccca ttcacggcaa tcttccggca aggcttcccg tcaaccgact
     4621 ggaaaatccg gccggtcatt ttgagtactg gacatccgac ggctggcaaa aggaagttac
     4681 cccgaaaaac gccaggatga tcttttcccc cggcctttcc gaactgagcg tccgctattt
     4741 ggcggaggat ggagaatggc tggctgttta cctgtcgccg gaaaacaagg gggatcggct
     4801 tctgtttgca acatcccggc aaccggaagg tccctggagc ccaccggccg ttcttattcc
     4861 ctccatcgat gaagtggacc cgaaaagccc gctgtaccat ccgcagacgt tctgctacgc
     4921 cggcaaggag catcgacaat tcgcccgcgg gagggaactc gttgcaacct acgtctgcaa
     4981 ttcaatggaa gacatcgatg atcaggatag ctttctgcgc aaaaatcttt ttctgtaccg
     5041 accgcaagta aaaaccatcc ggcgctgatt atttttgcta atccttgatg gtatagccct
     5101 tctcccggaa aaggcgcaaa caggcgtcca ccgcctcctc atcaaaaaga acgcccctgt
     5161 tttgggtaat ttcctgtaaa gccggttcca ggcctaacgc gggccggtag ggacggtgtg
     5221 tcgccatggc ttccaccacg tcggccaccg cgaggatccg cgattccggc agaatgttgt
     5281 ttcccgtcaa tccattgggg tatccggaac cgtcaatcct ttcatgatgc tccagaacca
     5341 tccttgcgat gggccaggaa aatttaatat ccttgagaat ttcatagccg gattgagcgt
     5401 gagttttaat cagattgaat tccaatttgg ttaatcttcg cggcgagcaa agaatctcgg
     5461 acggaacggt tttgatgccg ccagactgat cctttctttt tcttccagca gatccaccag
     5521 gattttgatc agaacttccg gttcctcggg cttgagaata aaacggtccg cgcccaaatc
     5581 cagcgcaaac ttctcatcct tgggctccgt ataagtggcc gtatagaaaa caaatggaat
     5641 attacgcaat atttcatctt tcttgcattc ccggtaaaag ctgtaaccat ccatcaccgg
     5701 catcaaaata tccgaaacta tcagatcagg ccgggaggcc cgggccattt ccagcgcggc
     5761 tttgccgttt tccgcctcac ggaccaacca gccctgagct tccagaatgc tttttaaaaa
     5821 gtataaattg acacgactgt cgtcagcgat caaaacagtt ttttcatgac gctacttttc
     5881 cctctcctgt ttcggcagat gctgttcttt ttccacttct ccttcttgtg gcaacattaa
     5941 gctaaaggtg ctgccctgtc cccagacgct ttccacccgg acttcaccac ccagcaaacg
     6001 cgccagcttc ctgcagatgg acaggcccag ccccgtgccg tcgtatttcc tcgttgtacc
     6061 ggattcaatc tgccgaaaag gattaaaaag aaccccgatg tcttccggtt tgatgccgat
     6121 tcccgaatcg gcaacagaga tcacaatttt ttcattttgg atttcgcatt tcatccggac
     6181 aaagccctgc tccgtgaact tgacggcgtt gctcagcaga ttcagtaaaa tctgctccac
     6241 ccgacggcgg tcgcttttca tcaggccgat ttccggcgcg atttccgctg tcagcttcag
     6301 attcttgttt tccgccagcg gctgagaggt tttaataact ttctccacgg tctcccgcag
     6361 actgaaggtt tcagagacca cgttgagctg ttccgcttca atcttggaaa tatccagaac
     6421 atcgttgatc aagctcagca aatgcttcgc gctgttgtaa accatcttga gctgcttgtc
     6481 ctgttcatcg tttaacggac cgcccaggcg ctgcagaaga ataccggtaa agccgatgat
     6541 ggaattgagc ggcgtgcgca gttcatgcga catggtcgcc agaaaagcgg atttcaggcg
     6601 atccgccgat tgcgcttttt ccatggcgag ggccaactcg gccgtccgct cgataatccg
     6661 cccttccatt tccgcattga ttttttgcaa ctccctggtg cgcgcattga cctgatactt
     6721 caggataacg ctgcccccca gactcatcag cagcacaata ccggtaatca aaccgagaat
     6781 ttgcagccac accggcagct tgaaacgcac ctcttcggat gtccattttt tcagggttgc
     6841 ataatacgct gattttgtgt tttttttcag gaaatcaaga tgtttgtcaa tggcgtccag
     6901 caattcctgg gattgatttt tcggggcggc gaaaaaaagc gcggacggtt caaaaacaat
     6961 tgccgtatcg accagatcat acttccgggc atgcatagag ccgtaaaatt gattggcaac
     7021 ggcagcgtca acctctcctt tggccaccat ttcaaacatg gttttataat ccgcaacggc
     7081 aacaagcttc gtatccaatc caaatccccg gctgaaacgg gtgaaagcct cctgctgaac
     7141 cgattgatcc agaatcgcga cacgtttgcc gttcaaatcc aatatggatt gtaatccgct
     7201 gcctttgcgg gcgtaggcct gaaaccagga ggacagcacc tgaattttgt gaaaggagaa
     7261 tttcttttcc cgctcagccg tcagggccac atccggcatc aaatcaatct ggcctttctc
     7321 caggcgctcc agtccctcgg cccaggtgcc cgccacatat tgcagttccc agtcttcttt
     7381 tgacgcgata tatttgataa tatcaacaaa gattccggaa ggccggccgg attcgtccgt
     7441 gaaaaccttg ggcgagtttt cataaacccc gatttttacc acccggcttt cggccagagc
     7501 catggaactg cacccgaatg aaaccagcgt cagaataaac aaccagcgca tcaggtgcgc
     7561 gaaaaagatg acggcttttc cttcagcatt tttccggtga catttgaaca taacatttct
     7621 tcctaatagt ttatggtcat ctgtaatccg acgagctgtc tggtccattc ccaccgactg
     7681 ttcacatttg aaaaatccat tctgaaataa ggtcctacgg aaacatgcgc cccgatattt
     7741 ttgttgatgc ccgcctttat tctgttgagc atcaccgcct tatcgcggtt ttcgtaaaac
     7801 agctcccatc cgacataggg ctgcaacaaa agatcgggga gggagatatt cagtgtattc
     7861 ttgattctgg cgtcattcca gtccggcgta attctgtatt catagcgaat ctgattgaat
     7921 accttgaaat taaaaaaagt tttggacagg ttcagattga tgctgggttt ttgctccaga
     7981 gcccagtgtc tgtcttcatc cttggaatac ccctgttgat aatacgccag aaaggaaagc
     8041 caggccaccg gcgtctggta ttgcaggccg atgccccatt ccgtatagtc ataattggac
     8101 atctcatcgt cagcctggat aaacacatag gaaacagcgc ggtatttttc attgaagttg
     8161 taggaaaaat tcaaatccgc gcgaaaagat gtgtcatgac ccgcataaag gaggggagca
     8221 ggcatcaaaa caaacaatga tgcaacaaga acaataccaa agcgcaaaaa acatattctt
     8281 cgcatcatca accctgtttc ttttatgtga cgctgtaaga aacctatcga gcatgcttca
     8341 catccatcag cctttgcctg tgaagatccc taacattcat ttgtgacgtt cagacgatgt
     8401 gagggattcc ctcaatcctt cagagctttt ttctccgtcg actgttcacg tttcggcagg
     8461 tgcaactcca cttccgacat gaacgtgtcc ggattgatgg gcttttcgat atacccgttg
     8521 cagccgacct ccatggcctt ttcacggtcg ccgaccatgg catgcgacgt cacggcaaca
     8581 atggggatgc ccgatacctg agggtcggcc cgcagatgtt tcgcgaccgt ataaccgtcc
     8641 atgaccggca gctgaatatc cagcagaatc aggtccggtt tcaactcggc cgccctgtca
     8701 atgcctgtct gcccgtccat ggcggcatgt accgtatagc cgtttttctc cagaataaac
     8761 cggaccagat aaaggttttg ttcattgtct tcaatgacca ggatattccc cttcatgcct
     8821 tgtctccttc ttgcggtaat gaaaatgtga aggtgctgcc ctgcccccaa acgctttccg
     8881 cctcgatctc accgcccagc aaatgagcca gcttcttgca gatggacagg cccagccccg
     8941 tgccgtcgta tttgcgcgtg aggccggatt caatctgccg gaaagcattg aagagcttgc
     9001 ccatgtcttc cggcttgatg ccgattcccg aatcggaaac caccagaaca attttacggt
     9061 ctttcacaaa gccgcgcagc cggacacctc cctgctcggt aaatttgacg gcattgctca
     9121 gcagattgag aaggatctgc tccacccggc ggcgatcgct gatgatcgtg ccgatatccg
     9181 gcgcgatgtc cgccgaaagc gatattcctt tgctgtccgc cagcggctgg gacgttttca
     9241 acacattctc cagcgactcg cgcagattga acgactcgtg ggcgacattg agctgttctg
     9301 cctcgatctt ggagagatcc aggacgtcgt tgatgagatt caacaaatgc ctggcgctgt
     9361 tataaaccat ggatagctgc ttgtcctgct cttcgttcag gggccctccc agacgctgca
     9421 gcaaaatgcc ggtaaaaccg ataatggaat tgagcggcgt gcgcagttcg tgggacatgg
     9481 tggccagaaa agccgatttc aaatggtccg cggattcggc acgttccttg gcgacttcca
     9541 gctccgcggt gcgctcgcgc acctgctcct ccagacggct gcgggaggtt ttttcagagt
     9601 cgcgaagcgc cgcctccgcg cgctttcgac gggacaggtt gaacagcagc gccagaatca
     9661 gcaccgcctg gagaagaata atcgccgcca ccgcaataat cttgcctctg ttttcctccc
     9721 agaaggtcac ctgccggtag cggatatcgc tgcggtcggg aagtctttcc ggggaaatct
     9781 tccatttctc gatttgccgc cagtcgtaga tgtcgcgggc ctccgcaacg gtttccgtaa
     9841 gcggcgcgtc cggccccgcc cgaagcagct tcagggccgt ttgagcggac gcttccccgt
     9901 aagcttccgc gctggacagc ttgccgccga caatgcccag tccgagaacc gtgtcgtaaa
     9961 aggagaagat cggcgccttc gaacgcggtg cgacggcttt catgacctgg accgtggtca
    10021 ggggcttccc atcccgatcc tgaagataaa cgagcatcag aacggccgat gcttccggga
    10081 ggcgctcgag cgtcgccgcc agttccccgg ccggcaggcc ctgcagatat tccacggttt
    10141 tgggccatcc ctttcccgca agagcctctt gggctacttt ttgatagttc agatcgtccg
    10201 cgccgctgcc gcacacgacg accagacgct gcgtttccgg gagaagaacg cgaatgcgct
    10261 cgatcgtatc cggtatggcg ttggcgccgc tccggaccag accggactgg ggtctgccgg
    10321 aaatcaccgc cagttgtttt ttggcgggaa gaacaaagac gacgggaatc cccgggaaaa
    10381 gcgactcccc gtggtccagg atgaactgac agctcacagg catgaaggcc aggaccatat
    10441 ctattttaat attggcatat ttctcccgga gaaaatccat cagcttgacg tcgtaatcct
    10501 catccgtcaa actctcactg ccgatgtatt cgtagctgat cttgctgtcg gaggacaagg
    10561 cggggaaaag ggttttccgc atggcgcgat tgagcgtatc gaaccagtcg gtcagatacg
    10621 gctgggaagt aataaccagg agatgctttt tagagttctg tccgtttgcg ccttgcgtat
    10681 ccggcggtgc ggcggcgcag gaaatcccgg cggaaaatat ctgcgacgcg cagcacacaa
    10741 aaagaatcgt caaaaacagg atgatcaggc gcgacggaaa ggttcgcaca aaatttctcg
    10801 gaacggacag ggagtctttt gtcatacacg tcatcgcctc tcagggttga agacaggatg
    10861 catgaaatca aaaataccgc tcgccaagtt tcctattaca ttcttcgggc aataaacatc
    10921 ccttttacag tccccgcata aattccgggc gaagcatgtt gggatcccgc cgcgacagca
    10981 gcgcctgaag ctgccggagg attttgcctt tgtccttcgg catcgaaccg cggatggaga
    11041 aataattgga agcgtgcatg gagctgaaaa agcagttgga aaaggtcgaa tgctccacca
    11101 tcgttttcaa ttccctgagc gagtcaaacg gcgtgatcat ttcaaaccgg ccgctctccc
    11161 actctttgta aatctccgtt tcgggaatca gcgtcagtgt cagggcgccg gcgtaatccg
    11221 ggtccatctc cgtcagaatt ctcgccgtct ccagcgcgtg tttctcgctt cccgcgacgc
    11281 cgcccagccc gaggatcacg gtgacggaca ggagaatgcc ggaggccttc acctttttgg
    11341 cggcctccac catctggtca tgactgacgt ttttccggat cttcttcagg acgtcgtcgt
    11401 cgccgctttc cacccccatg taaagaatgc cgagtttctg ctttttcaga tcccgcaact
    11461 cctgaacgct tcgccgcaaa acatcctgcg gcgtggcgta gctggcaatg cgctccaggg
    11521 cgggaaattt cccattgaga tactgaagag cctccatcag ccggggatac ggataaacca
    11581 gagcgtcgcc gtcctggaga aacacccttt tgccgtccca ctggcggagg atggcataga
    11641 cgatgtccgg ctggcccgcc atccacatgc ggctttttgc atacagggac atggcgtcaa
    11701 tttcacgctt cacgtcatcg aggtcgcgaa tgccgagagt cgtgaaggaa aacgcgcaaa
    11761 aggcgcagga attgttggaa caccccgacg tcaggggcag ataatagctg gcatgctcgc
    11821 tggggggtcg aatgatatcc ggtctgacaa atgacatggt tggatcctgt aaatttattt
    11881 tgtgtcaaca tacagatacg accgtccgtt tgtcaagacc gtcttgacgg tcaatgattt
    11941 ttcttgcaac gttgcttcaa agctgtaata tagcgccggt ttaaaaaagt cctttgcggg
    12001 cgtaaaaaaa tcggcgctgc gccacgccca caaagaaaat ttgaagaagg agaaacacta
    12061 tgagtttaga ccccttattt tatcccaaaa gcgtcgccgt catcggcgca tccgacaatc
    12121 tgggcggcgg caaactgccc tatttccaga ttctgcaggc aaacggcttt accggcgctc
    12181 tttatcctgt caacccccgc cgcgttgaag tgtccggcgt gaagacctat gcgtccatcg
    12241 acgacctccc ggagcccgtt gattttgcca tcgtcgcggc gcccgtcgag cagtccgtgg
    12301 acattgtcaa agcggccgtc cgcaaacaaa tcaagtttat tcatttcttc acatccggct
    12361 tcggagaaac ggggaatctt caactggagc aggagatcat ccgggaagcc cgcaagggac
    12421 atctccggat cgtcggcccc aactgcatcg gcgttcaatg cttcgaatcg aaagtttctt
    12481 tccgactgac gaatctgcag gaagggcccg gttcaatcgc ctttttggga cagtccggcg
    12541 gaatcacgtc cagctttgtg gccatggcaa ccacccggga catccggctc aacaaagtcg
    12601 tctcctacgg aaaccagatc gatctgcgcc tggaggatta tctggagtat ctggcggatg
    12661 acgacaagat cagcctgatc gcgtgctacg tggaagacat caaggacgtg aacaaatttc
    12721 tgaaagcgct gaaacgcacg acggccaaaa agccggtaat cattctcaag ggaggcaaga
    12781 ccgaactggg ttcgaaggcg gcggccagcc acacgggcgc catggccagc aattacacga
    12841 tctgggccgc ggccgtacgg cagcacggcg gcctgctggt ggataatttc gaagagttga
    12901 tgaatctggc gatgctgggc accgcgcagc gattcccgcg cgggaggcgc gtgggattca
    12961 tgggcgccgg cggcggcatc tccgtgctgt tctccgacct ggccgctgcc tcgggggtat
    13021 tgcttcccga actgagcccc cgcacgcagc ggatgatcag cgaaaaaatc atgggcgtca
    13081 acacctcgac gaccaatccc gtcgatctcg gagccttcgg tttcgacttc aacgtgatgc
    13141 tgcacaccat gaaagccctg aacgaagacg acaacatcga tgtgatcgtt ccctattttt
    13201 ccgttgaata tatcatgcac gcggaattgt ttctcaaggt gaccaacagc gcgcacagca
    13261 tcatggaaat ggcccggcaa atcaaaaagc cggtgatccc cgttctgtcg tgttttctgg
    13321 aaaatgatct ggaggccgag cgcgtccgga tttccacctt caacgcgctg cgcaaggcgg
    13381 gattccccgt ttatccaacc attcaggagg cgctttacgt gatcgaaacc tatttcgaat
    13441 gggcggaaaa gcggccgcgc cgtggcgaga aaaacgctgc ctagcgaaat ggcccgagcc
    13501 gctttcaggc cacgcggcaa aagaagactg ttgaagattt gagatgatga aaacaaaagg
    13561 ggagagaagc cgatgaagaa aatcatgatt tgtctcgctg tcgctttgtg ttttttgtcc
    13621 tgcgggtggg tttgcgccgc agaaaatccc ggtcaaccgg tccccgtcgg aagcgctttt
    13681 gccatgccgg cgcacagtcc atcctatcct cccgggccct atcgcttcat cgacagggaa
    13741 tttctcatca tcacctacga aaccgacatg gaagcgctgc gcaaacaggt acccgagcct
    13801 ttggaaatcc ccgaagcgct ggtcaaattt gagttcatca aaatgcccga ttccagcggg
    13861 ttcggaagct acaccgaagc cggccaggtt gtcccggtca tcttcaaagg acaaaaagga
    13921 cagtatgtgc tttccatgtt tctggatgat gaggcgccga ttgtcgcggg gcgcgaaatc
    13981 tggggcttcc cgaaaaaact ggcttacccg tcgctgggcg tcgatcccgt cggcagggac
    14041 accctggtgg gacgcctgaa gtacggctcc gtggaggttg ccgtcggcac catgggattc
    14101 aaatacgcac ctgttgacgg ccaggccatt aaaaaatcgc tggaagacgt tcccaattat
    14161 ctcctcaaaa tcatccccgg cccggacggc tccaccgtcg tccggcagtt ggtgcagtac
    14221 aacctcaagg acgtgacggt caagggcgcc tggacagggc ccgccgccct tcagttattt
    14281 catcacgccc tggcgccggt ggcgtccctc cccgtcaaaa gaattgtgtc gtgcgttcat
    14341 tttacatccg acctgacgct gccttacggg aaagtggtcc atgattattt gaaaaaatag
    14401 cgcatcaccg accggaagga gcaagcggca catgaatcga ccgtctcttt ttcatcccgc
    14461 cccgcctctt gccgccctgg ccatcgtcct gatttttctc ttctccgggg tagtcctcgc
    14521 cgccgcacag gaaatcgcac cggccgcgga gactgccttt ccgacccgcg actacctgac
    14581 cgggaactgg agcggcgtac gggaccgcct gcatcattcg ggcgtccggg tcggtctgaa
    14641 ctactcgacg gaaaccatgt tcaatgttgc aggcggcgaa aagcagggcg ggacctacgc
    14701 cgacaacatc gaccttgcgc tcctgttcga tctggcaaag cttaccggca ttccccgcac
    14761 cacgtttctg gtcaagatgt cgcagcgcga cggcgacagt gtgtcggcgc gcttcatcgc
    14821 gccatcccag gacggaaaca cctttcccgt tcaggaactc tacggcgggc agaccttcaa
    14881 gctggtgaac gtgcagttca acacccgcct cgcggacgac cggatcgatt tagcctacgg
    14941 ccgtctggtc gccaacgatg actttctccg ctcggatctt tactgccagt tcctcaacaa
    15001 ctccttctgc ggcagcccga agccggtttt tctccaaaac cccttcacat tcaccgccta
    15061 cccgctggcc acgtggggcg cgcgggcggc cctcgacaca ccgtcgcgca actggacctt
    15121 ccaggccgcc ctctacgacg gagatccgga gggaagaaga ggcgacccgg ccggtgaaaa
    15181 ccaaaacctg cacggcacct ggtggaaatt cggcaacaac ggcgtcacgc tggcgggcga
    15241 aatccgctac cacgtgaacc gcggctcgga cagcgctctg ccgggcgtgt acaagatcgg
    15301 cggattttac atgaccggcc ggtttctgga cgtaagcaga atgggaaacg tcaccacaac
    15361 cggtgacgcg atgatctggc tgctcgcgga tcagatgctc taccgcgaat cgcccggcga
    15421 aagacgggga ctttccgcct tcggctcgct ggtcctgagc ctcacggaca agaccaatcc
    15481 gatgacgagc tacttcaatg ccgggctggt ctatgagggg ctcttctgca cccgtccacg
    15541 ggatcggacg gggctggccg tcaccgcggg ctttttcgga aaagactaca aggacggcct
    15601 ccgggccgcc ggactggaga cgaaaagcca cgaggccgtc atcgagttca accacctgtt
    15661 cctcgtcggc cgtggaatcg gcattcagcc ggacatccag tacatcatcc gtccggcggg
    15721 aaccggcgcc atttccaatg cgctggccgt cggcgtgaag gtcagtgtag atttttaaat
    15781 ccgggatttc ggggaggtga catgagatca ggatacaaga ccactgtttt ttacatttgc
    15841 ggaaccgcgg cgctgggcgg ccttctcttc ggactggatc agggattcat cgccaattcc
    15901 ctggacacga tccggaaggt ttacggactg gacattcggt ccgcagaaca ttattcctcc
    15961 attctggcaa ccggcggcgt catcggcgcg cttctctcgg gcctcctggc ccggtatctg
    16021 gggcgtaaaa gaagtctggt gctttcggga ttcatcttct gcgcttcttc ccttgtctcc
    16081 gcgctgcttc ccccgctggc agtcatgtac gtctgccggt tcctgctggg attcgccatc
    16141 ggcatcgcct cctttatcgt accgctgtac ctgtcggaaa cggcccccgc ttcaattcgc
    16201 ggcgccatgg gaacgctgtt tcaactgctc atcaccatcg gcatcttttt gatctccctg
    16261 accaatttca tcatcgccca gtggatctca tcccccgaga cggcgcttcc cctgatgttt
    16321 tccgtgatcg ttgtgtttgc cggcctgatg ttcgcgggga gcctttttct gccggagagt
    16381 ccgcggtggc tcatgttgaa gggaagaaaa gatgaggcaa cggcggttct tcaaaaaacg
    16441 ctcaacaccc cggaagacgt cctgcgcgaa attcatgata tggaggcgac cttaggccgc
    16501 cggggtttcc ggatcggcaa cctgttgaaa ggctacttca tcaaaatcct gctggtggga
    16561 attttcctgc aggtgttcca gcagctggtg ggaatcaacg taatgattta ctacgccccg
    16621 accattttcg gatatgcggg actggccggc atgatcgcga tgatgaccgt gccgacggtc
    16681 aacatgctct ttacctttcc cgccatccgg cttgtggaaa aatggggccg aaagaagctc
    16741 ctttacgtag gcgcactttg catgatgatc agcatgacgg cggcgggcgg agcttttgct
    16801 tatatcggca acccgtccga cccggcggcg atcggattag cgcccaaggg cattttgctg
    16861 gccgcggtca ttttctacat cttcgggttt gccgtttcct gggggccggt ggcctggctg
    16921 gtctgctcgg agatctttcc gatcaggggg cgcgaagtgg gaatgaccgt gacgaccatg
    16981 gtgaactgga ccttcgccgg tctgctgatg gacaacgccc ttacttttat ggaggctcac
    17041 ggccatgcct ccatcttttt cctgtttgcc ttcttctgca tcctggccat cgccttcgtc
    17101 gcggcttttg ttccggaaac gcggggagtg acgctggagc agatcgaggg aaatctcaag
    17161 gccgggaaaa agatgaggga tttgggagat taaaaaatga aaaccgtaag ctatcgatcg
    17221 ctgggtttgt cgaacaccgg ggaaatgttt cagaaggcct ttactgaaaa gtatgccgtt
    17281 cccgcttata attttaacaa catggaacag cttcaggcca tcattgccgc ctgcgttcaa
    17341 accgcgtccc ccgtcattct gcaggtatcc ggcggcgccc ggaaatacgc ggatcagacc
    17401 cttctgcggt atatggcgca aggcgccgtc gcctttgccc gaaggatggg aggcacgatc
    17461 cccatcgcac tgcatctaga ccacggcgac agttttgaac tggcaaaatc ctgtatcgac
    17521 agtggatttt catccgtcat gatcgacggg tcacattttc cctttgaaga caatattgcc
    17581 ctgtcgaaaa gagtcgtgga atatgcgcat ccgcggaatg tctcggtgga agctgaactg
    17641 ggcgtccttg ccggaatcga agatgaagtt tcccacgcac agtcgcatta tacaaacccc
    17701 gaggatgtgg aagaattttc cggcagaaca ggcgtggaca gcctggcgat ctccatcggg
    17761 acgagtcacg gcgcgttcaa gtttaaaccc caagactgca ccctgaatgc agatggggtt
    17821 ctggttcccc cttcgctccg ttttgatatt ctggaggaga tcgaaaaacg cattcccgga
    17881 tttcccattg ttctgcatgg tgcatcgtcg gttgtaccga agtatgtcgc gatgatcaat
    17941 caatacggcg gccggctgga agcggccgtc ggcattcccg aagagcagct tcgccgcgcc
    18001 gccgaaagcg ccgtttgcaa aatcaatatt gacagcgacg gccggctggc catgaccgcg
    18061 gcgattcgca aagttttcgc tgaaaacccc ggtgagtttg acccacgcaa gtatctcgga
    18121 cctgcgcgcg ccgagttgat cgaactgatc aagcacaaga acatcaatgt tttgggcagc
    18181 gcgggccggt acccggccct gccgtagtcg ccggactccg gaaaacgcgc ctgcctccgg
    18241 ggcggatccc ccccccagag caagctgtcg agggatgaaa tgaaggtcat gatatcgcgg
    18301 caggctgcgg aggatgactg ctcgtcccgc aacggcggga ttcaatcgaa atagcttcgg
    18361 taggcctccg ggagggcgtc ccggttgacc tgaaacagct cgatgagcac gtgatcggga
    18421 gccggcacca tgacgtattt ccatacgccg atgtccgtga cgcccttttt aaaaggcacg
    18481 cccatggcct tgagcttttc gaccacgcct tcgatatcgt ccgtctgaat cccgaagtgg
    18541 tggatgcttc ccctgccgga aaaggaaggc gcctgatcgt agaaatgaat ccgcccggtg
    18601 ccgactctca tgaacacgtt tcttgccccg gccatattct catccagaat aatttttccg
    18661 ccaaaggcgt tttgataaaa ggccaggctc ttttcgatat ccgacgcaaa gagatgtacg
    18721 tggtggacat ggttcatgga tgttctcctt ttctcccggg cgcccgttcg cgcttttgcc
    18781 cggcttgcat tttttaacgt caaccaagct gtcaggtcag cctgcaagcg cgctgcgtct
    18841 ttttttcaac ctgatgtaag gccggattcc ccggtctctc cttgcgccgt cagggaacgg
    18901 atcaacgcgg ctgcgttgtc gtaaaaaatc cgatcgtaaa attccggaga aagattcatt
    18961 ttcagaagat tttcaatctc cgcttcccac ggcgtgatca ggttgggata atccgatccg
    19021 tataaaatcc tggatttgta tttttcaaga tacccggggc cgaggttgta tccgtcgtag
    19081 ggcgcgggca aaaaacagaa ggcggtgtca agatacagct gcggatgccg gtccacaagc
    19141 tccatgaaac cggcatattc atgtgctccc agatgggcga tggtagcggg caggtcggga
    19201 aaccgatcca gaactttcat gaacggctca aggccgacat agggattccc caccgggccg
    19261 gttccggtgt gaaaaagaat gcgcttgttc tttttcatca ccaggtcgta gaggccgaag
    19321 agccgctcgt cgaacggata aaaattctga accagaagct ggagcttgaa tccaaggatt
    19381 ttcggatttt ccacaaggcg acgggccatg tccagagcgt ggtcgtcgcc tggatgatac
    19441 gcggcgaagc agtacagatc ctcatactcc tgaaggactt tctcgttcca tcggttcagt
    19501 tcttccgcaa tgccggcgcg gtgcgcgtag ttggaatata cgatcacgct cacgcccttc
    19561 cgccgaaggc gctcgatgca ctcgcggtaa taacagcgat gaataatatg ccagttatac
    19621 tgcttcagaa aggcttccca aatggcatcg aaaagtctgt ccggaaacag gtgcacgtgg
    19681 aaatcaatta ttttttcagg aattccactc atcgtctcca ccacgtcggt tattttgcgt
    19741 ctggttaaca caaccaggtc tcataatcaa cgaaaaggtc atccgagcaa ggcccgcatt
    19801 gccgcttcac tcccctttca ggcaaagcgg agtccttgac ccgaagaaaa atgtaggtta
    19861 gtaataaacc cggaaacggg aaaccatgga acctgttttc gggaggatga gatgaaaagg
    19921 aaatgggctg tccttttggt ggtgatggcc atcgccgccg gcgccgggcc ggcgcgcgcc
    19981 gaaataacgc agtccgacct cgtggtcctc gatgtggcgc tgatccggcc ggtcggcatc
    20041 gtctccgttg tcgtcggcac ggctttgttc attctttcgc ttcccgtgtc cattcccagc
    20101 ggcagcgtcg gcactgcggc gaacagtctc gtgaaagaca gcttcgaata tacattcatc
    20161 cggcccgtcg gggatttcga ctatgagctg ggaacatggg gcaaagaggc gccgccctct
    20221 tccgaaagct caggcgccgc ggcaaaatag gctatagaca aaaaagcttt cacccgttat
    20281 aaggaaaacc atggaaaccc ttctcgacac acaactggcc gtcccccttt ctcaggtggc
    20341 cctgctcctg gggttgagca ccctgatcct gcttttcggg cgggtaaagc tggccctcat
    20401 catcaattac tgcttcacgc tgtactgggg gttttttctg aatcccagct tccgttccga
    20461 tctgggagaa ctcatgctca acacctacac ctatgtttat atcggggttg gtctgatcat
    20521 tgttgttctg gccctgatcg gttttctgtc cagcaaggac agatgaggca tctttccaaa
    20581 atgattagtg atttcattgc gttatgaacg gcattccctg aattgtccct ctgaacctgt
    20641 tccccggcag gactccgcgg gaaccggcgc atccccgcag ttttggtcaa gatgttcttg
    20701 acaatcccaa aatgacttcc tataaaaagc gca
//
LOCUS       DAOSRM010000031        20589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2779_length_20589_cov_97.725821, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000031 DAOSRM010000000
VERSION     DAOSRM010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 20589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20589
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2779_length_20589_cov_97.725821"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLA58_04665"
     CDS             <1..368
                     /locus_tag="PLA58_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOR62120.1"
                     /translation="ARAAWRASMEAFHARPVEADLFPGSDIPREAPQADTSAIPRYAI
                     TAEDLAAGLLISSLCAKAGLTQSMSEAKRLIGQGGIYVGERQVKSPDEKLSPDDFGGG
                     RELRIRKGKKKYLMVEIGD"
     gene            592..1137
                     /locus_tag="PLA58_04670"
     CDS             592..1137
                     /locus_tag="PLA58_04670"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOR62121.1"
                     /translation="MEFKTVLEKLLTNFERLNIRYALMGGFALGLWGYGRATADVDFL
                     AHRDDMDKVDAVMKSLGYECLYKSDNVSQYLSDLKIFGEVDFIHAFRATSLAMLETAE
                     EKMVFNGDLKIRVLKPESLIAFKLQAVKNNPSRTSVDLQDIKKLAEINKRDMDCGLID
                     RYAELLNMEEVWHEIKKEVFP"
     gene            1134..1343
                     /locus_tag="PLA58_04675"
     CDS             1134..1343
                     /locus_tag="PLA58_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62122.1"
                     /translation="MNPAPGGEDMLDQTITEDIKAMESRRHNPFMKNGKADVDAYIRF
                     VCGFNEFINHDPKPFSQMMDRDMIL"
     gene            complement(1409..1834)
                     /locus_tag="PLA58_04680"
     CDS             complement(1409..1834)
                     /locus_tag="PLA58_04680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOR62123.1"
                     /translation="MDEKIRKAIFQRMEQEPFGRKFGITLVDLKEGHARVEMRFTSDM
                     ENMFGMAHGGAIFSLMDAAFEAASNSHGTMAVALSMSIQYLASPPKGSLVNAEAREIN
                     KTKRTAAYDIRATDESGNLLAACQALVYRMEKPLPFLEH"
     gene            complement(2051..3214)
                     /locus_tag="PLA58_04685"
     CDS             complement(2051..3214)
                     /locus_tag="PLA58_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOR62124.1"
                     /translation="MYRIDLLNKISEKGLSLFTDKYEYRTDIPDPDGILVRSKEMKEM
                     TLPSSLKAIARAGAGVNNIPIDKCSEKGIVVFNTPGANANGVKELVVLGMLLAARNVA
                     EGLIWAKGLVGEGDKVPDLIEKGKAQFVGTEILGKKLGVVGLGAIGTMIANTAEALGM
                     DVMGYDPFLSIEAAWKLSRNIKKAPSLDRLLAESDYITLHVPQNKDTKGFINADKFAI
                     MKKGVVLLNFARGGLVETAALKKAIADGIVSRYVTDFPDEEIIKTDKVIAIPHLGAST
                     EESEENCAIMATMQIMDFLENGNIKNSVNFPECILDRSDKTRITVSNKNEPGMIEKIT
                     HLLADNKLNIADMINKSRGNIAYNIIDVEGDINAAELVKKFEAVNGIIGVRIL"
     gene            complement(3240..3314)
                     /locus_tag="PLA58_04690"
     tRNA            complement(3240..3314)
                     /locus_tag="PLA58_04690"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3280..3282),aa:Lys,seq:ttt)
     gene            complement(3390..4688)
                     /locus_tag="PLA58_04695"
     CDS             complement(3390..4688)
                     /locus_tag="PLA58_04695"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOR62125.1"
                     /translation="MANVAVVGAQWGDEGKGKIVDLYTLKADVVARFQGGNNAGHTLV
                     VKGRTTILHLIPSGILHDHKICIIGNGVVFDPKVFLQELKELNEGGLFPSSTKLYVSE
                     KTHVIMPYHRSLDQAREARNTGKKIGTTGRGIGPAYEDKVARTGIRVGDLYEPGLLLE
                     KVRHSLEEKNYLFKNFFGVAPLDPQAVADEYLAYAEKIRPYVADTSLILAGEIQKGKK
                     ILFEGAQGCHLDVDHGTYPYVTSSNTVSGNAACGSGVGPGAISKVVGICKAYTTRVGE
                     GPFPTELKDAIGDHLQKIGQEFGATTGRKRRCGWLDMVLVRQAVRVSGISTLAITKLD
                     VLTGLPKIKICVGYKTAGGECREAVPASLKRFSTCEPVYEEFDGWTENIASAQNISEL
                     PVNARKYIQRLKELSGAGIALVSVGAGRDETIQLENPFEN"
     gene            complement(4704..6287)
                     /gene="serA"
                     /locus_tag="PLA58_04700"
     CDS             complement(4704..6287)
                     /gene="serA"
                     /locus_tag="PLA58_04700"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOR62126.1"
                     /translation="MKRVLVSDSMAPEVAAILGNTPGIKVDVKTGMKPDELKAVIKDY
                     DALIVRSSTKVTAEIIEAAAKLSAIGRAGAGVDNIDIPAASKRGIVVMNTPGGNTVTT
                     AEHAIAMMLALARRIPQATASMKAGRWEKSRFMGNEYCNKTLGVIGLGRVGAVVADRA
                     IGLKMNVIAHDPFISPEVAQQMGVNMVNLDELFTNSDFISVHCPLSKETRNLINESAL
                     AKMKKGVFLIHCARGGIVNEKALCRALKAGQVAGAAIDVFEEEPTKNMELVGLDNVIC
                     TPHLGASTDEAQTNVAIAIAHQIAAYFTTGEIKGAVNFPSVSADVMAGIRPYLDLAEK
                     LGAFQAQLLTGAIQEVSLEYSGEILNHNVAPITVSLIKGLLDQILTETVNYINAPVLA
                     KERGIRIIEVKSSETKDYTNLIALTVRTSKETSRTSGAVFGQNDPRIVRINEFSVEIA
                     PEGYLLAVSNKDVPGVIGNLGTTLGSHNVNIARLHLSRDAQSKKALVVLNTDSPVGPD
                     ILDKLRKLPHVVSITPIKM"
     gene            complement(6312..7439)
                     /locus_tag="PLA58_04705"
     CDS             complement(6312..7439)
                     /locus_tag="PLA58_04705"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HOR62127.1"
                     /translation="MLNPNFSSGPCSKRPAWNIDALKSAPVGRSHRSALGKERIVKAM
                     NDTRKVLNIPSDYLVGILPASDTGAFECAMWSLLGPRPVTVLVWESFSEGWATDVNKQ
                     LKLNPTILKADYGKIPDLSKVDWKSDVVFVANGTTSGVRIPDWNWIPADREGLSLCDA
                     TSYTFANEVDWSKIDVLTYSWQKCLGGEAAHGMMILSPRAVARLESYTPPWPMPKIFR
                     LKKGDKINKAIFEGDVINTPSMLCVEDYIDALEWAGTFGIDGLIKKSKANLKVVEDWV
                     AKTDWIEFLATDSKTRSNTSVCLSVTHPKVAAMDADAKSKFLKSIASDLSKKNIAHDI
                     NSYKDAPAGYRFWCGPTIEEADLKIALAELEKVFNEKVKSL"
     gene            7692..8027
                     /locus_tag="PLA58_04710"
     CDS             7692..8027
                     /locus_tag="PLA58_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOR62128.1"
                     /translation="MTENLAKIADLTGAFRLLELVDYQGRSVVSREIIRKPFGTMTVF
                     AFDAGEGLSEHTAPFDALVYLLEGKAEITIGGKPHAVGEGEMILMPAHKPHALKAVTR
                     FKMLLVMIK"
     gene            8046..9233
                     /locus_tag="PLA58_04715"
     CDS             8046..9233
                     /locus_tag="PLA58_04715"
                     /inference="COORDINATES: protein motif:HMM:NF008887.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HOR62129.1"
                     /translation="MKFEITPGVDWVGKIDWELRKFHGEEYSTHRGSSYNAYLVRDEK
                     TALIDTVWSPFAKEFVDNLAKEIDLNKIDYVIANHAEMDHSGALPLLMHRIPGKPVYC
                     TANGLKSLKGHYHKDWNFQVVKTGDKLSLGGKELIFVEAMMLHWPDSMMEYLTGDAIL
                     FSNDAFGQHIATEHMFNDLVEPSELFEEATKYYANILTPFSPLVTKKINEVVGLNLPV
                     NFICPSHGVIWRDNPLQIARKYLQWAADYQENQISIIYDTMWDGTRMLAEHIARGIKQ
                     ADDKVTVKLFNISRRDINDVVTEVFKSKAILLGSPTINKGILNAMSALIEMIAGLKFK
                     KKKATAFGCYGWSGESVKILTDHLSKAGFEMIGDGFKAMWQPGDEALAQALAFGKDFA
                     GKV"
     gene            complement(9316..11616)
                     /locus_tag="PLA58_04720"
     CDS             complement(9316..11616)
                     /locus_tag="PLA58_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOR62130.1"
                     /translation="MIMKTKPPIEVSLEERLRLQKKLQDITNRIHAAQNIAQILIDLK
                     DGILNLFDAYSITLYVVDKPHNEIYSMFLAGAQLKEIRVPINNHSIAGYVANTFNIVN
                     IANAYNLAELKAIDPELTFDASWDKKSGFKTKQILAAPIFYKDQLMGVIQILNKKRGD
                     SKFTEEDIGLVMEIVEVLGVAFYNQQRFQRRRKTRFDFLLTRNLLKEDELEAAWGEAR
                     EQKESVERYLMNKYKISKDDIGHSFEEFYRCRFIHYNEKIIIPGDLIANLKKDYLQRE
                     LWVPLEKIDGNIHVIVDDPNNIIKRDAIENLLKTKKVKYDVALPEDILKFINLFFSSG
                     EDEHSFTEILGKMDEEGGGAEEEEGVDVVAETDSVIMQLVNKIINDASSRRASDIHIE
                     PNVGKKNVEIRYRIDGDCTIYQTVPFSYRSAIISRVKIMSNLDITEKRKPQDGKIKFR
                     RPSGDELELRVATIPTQGGVEDIVMRLLAKSDTLLPLESMGLLKRNYDGMIDIINKPY
                     GMILVVGPTGSGKTTTLHAALHVINRPDRKIWTAEDPVEITQYGLRQVQVQPKIGFDF
                     AAAMRAFLRADPDVIMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETIVRLLDM
                     GIDPLNFADALLGILAQRLVRTLCKNCKEQYHATEEEFEEIVESYGREDFAKLGVSYT
                     DDFKLYRAKGCDACDKTGYKGRMGIHELIVATDAIKKLVQKHETVEVIRAKSMAEGMT
                     TLLQDGIQKVLQGITDFNQVRRVCIK"
     gene            complement(11745..13817)
                     /gene="glyS"
                     /locus_tag="PLA58_04725"
     CDS             complement(11745..13817)
                     /gene="glyS"
                     /locus_tag="PLA58_04725"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOR62131.1"
                     /translation="MSNELLFEIGTEEIPAGFLSKAVVDMEEMIRKALSEKRIAFEGI
                     RCLATPRRLVLHIADLAPKQDDQVVEKMGPAKKAAFDENGQPTKAALGFARGQGLEVS
                     QLETVTTDKGEYLCARKTIAGQPTADLLPDLLKSFLLAIPFRKSMRWAAYDLRFARPI
                     HWLLALYGGKVVPLKVEDIESGNTSRGHRFMSPEAFAVEGYEDYLAKAQERFVIADPA
                     ERKKLILEEAQKSAADVGGKLFYTEDLLETITFIVEYPVIVRGGFDPHYLQIPKDVLT
                     TTMISHQKYFPIVDDAGKLLPYFVAVSNTRARDLEVVARGNERVLQARLADASFFFEE
                     DKKVPLEDRVESLKKVVFHTLLGTSHKKVMRFRKLAVKIAAKVKPAVKKNVDRAALLA
                     KADLESLMVGEFSELQGIMGREYALLAGEKQEIADAIYEHYLPIVAGGDLPGTDEGAI
                     VGIADKMDTIVGFFAVGMPPSGTADPYALRRQALGVINIILSRRYDLSLNFLIDECLA
                     SLKDVLRKPAEDVKKDVLEFFRGRLQNQLISQGYAYDTVDAVLAADIDRLVLVIEKIQ
                     ALQAFRANPEFEPLSIAFKRIDNILKDFHGGSVDVNLLSQEAEIKLFANFENIGTRVE
                     KGIAEKDFTAALNKLAALRSPVDAFFDSVMVMDKDEKIRFNRLSLLADISALFHQIAD
                     FSKIVTAG"
     gene            complement(13834..14706)
                     /gene="glyQ"
                     /locus_tag="PLA58_04730"
     CDS             complement(13834..14706)
                     /gene="glyQ"
                     /locus_tag="PLA58_04730"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOR62132.1"
                     /translation="MTFQELIFALEQYWHSQGCVIQQPYDMEVGAGTFHPATCLRALG
                     PEPWNVAYVQPSRRPTDGRYGENPNRLQHYYQYQVIMKPSPVNIQELYLDSLKSFGID
                     PLEHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVGGFDCKPVCAELTYG
                     TERIAMYIQGINNVYDLQWTDNIKYGDVHHKGEVEFSTYNFEVADIPMLRKLFDMYEE
                     EALRIAEKNLSLPAYDYCLKCSHTFNLLNARGAISVAERTSYIGRVRNLARISAELYM
                     KQREELGYPLLQNQ"
     gene            15117..>15320
                     /locus_tag="PLA58_04735"
     CDS             15117..>15320
                     /locus_tag="PLA58_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62133.1"
                     /translation="MPAYSGMTNVAKYLQRGHQRRISYLVAKIFLTSYKASFTLHAHK
                     QEVFTNYQFINPKEGEFYDRKGIR"
     assembly_gap    15321..15420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15456..17099
                     /locus_tag="PLA58_04740"
     CDS             15456..17099
                     /locus_tag="PLA58_04740"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HOR62134.1"
                     /translation="MTGKVYERGECYKYQLLIKHLLETPLFYAPEQEIVYRDKVRLTY
                     RQLNERIHRLANGLEKLGVKKGDTVCVFDYDSHRFLECFFAIPMMGAVLHTQNWRLSP
                     EQILYTMNHAGDDVVIINSDFLPLLEAVKDKLKTVKKIILIDEDGTKPATALKIDVEY
                     EAMLAASATFYDFPEFDEQTRATIFYTTGTTGDPKGVYFSHRQIVLHAQALLLVTSAY
                     DGIGRFNSSDVYMPITPMFHVHAWGVPFAATMLGIKQVYPGKYEPAMLLKLILTEKVT
                     FSHCVPTIIQMLVSTPVVKQFDLTKWKVIIGGARLPKGLAQAAMEIGIQIYAAYGMSE
                     TCPLVSMANLKPDKVGKGMIENVDTLIRTGLPAPLVYARIMGPDGKLLPHDGETTGEL
                     VLRAPWLTEGYYKEPEKSKDLWPDGWMHTGDVAYIDPEGYIQITDRIKDVIKTGGEWV
                     SSLELENLMSLHEAVAEAAAIGVPDAKWGERPMLILTLKPDFKGKITEEDFKRYMMEA
                     STAGKIPKYGVPDRYVMVDQIPKTSVGKIDKITLRKQFQ"
     gene            17494..18648
                     /locus_tag="PLA58_04745"
     CDS             17494..18648
                     /locus_tag="PLA58_04745"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR62135.1"
                     /translation="MLNFTFQNPTKVIFGRDAISKIAGEVPRDARLMVAFGAQSARKY
                     GVLDEVLRALAGFDVTEFGGIGPNPEYETLCSGIALAREKKIDFLLAVGGGSVIDGTK
                     FMAAAIPFSGEPYLLMENKGAAIRRAMPLGAVLTLPASGSEMNNRAIISRRAANWKRA
                     IMNDFLFPRFAVLDPTKTFTLPPAETGNGVVDTFVHVLEQYLTYPVDGKVQDRLAEGL
                     LLALIEEGPKALREPENYDVRANLMWASTLGLNGLIGAGVPQDWAAHRAGYELTVLYG
                     LAHAQTLAVLVPAMLKVRSTGKKQKLLQYGERVWGISSGAETLRIDAAIERTRTFFET
                     MGLPTRFADCGIKNVDADRVVELLAEHGLITMGESGDVTPGTIREILALC"
     gene            18728..20386
                     /locus_tag="PLA58_04750"
     CDS             18728..20386
                     /locus_tag="PLA58_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOR62136.1"
                     /translation="MHSILPEIGIAVLAATAMGFIFQLCRQPVILGYLVAGALIGPQI
                     GFKLVSDPANIEVISEIGLILLLFIIGLELNPAKLLSSGKKLIYAGVGQFVLCVLIGL
                     GFFVLLGYPLGGGRIEALYLSLFCALSSTAIVVKLLYDKFELDTLPGRISLGILIFQD
                     LWAILILALQPNFMDPKLHLVALALGKGVLLLAVGFLLSRFALRWIFAKIAKTPEMVV
                     ATSIAWCAFMAGLGSWIGMSMEMGALIAGAAIASFPYGVHVTAKVLPLRDFFLTLFFL
                     SIGMKIPFPDAATLTMAVLIVLFVIASRFLTIYPILQLSGSGRRTSFITSLNLAQISE
                     FSLVVAALGVQYGHIQQSLMSLIIYAMAITSVLSSYFIKGNHTIYLAFDRVLTRMGFP
                     SKAARETAEEEQDHYPIVLLGYHRGARAIVEKIQEDAPDLLEKILVIDFNLEVLKELE
                     DRHVKGVFGDISSMDTLDHAHVAGAEVILSTIPDMLLKNTSNLSLVKTCRMLAPNALI
                     VATADSAEQAEELKTSGANEVLLPYSLIGEAIAEMLLDWQRKGA"
     gene            20513..20588
                     /locus_tag="PLA58_04755"
     tRNA            20513..20588
                     /locus_tag="PLA58_04755"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20546..20548,aa:Asn,seq:gtt)
BASE COUNT         4263 a         5556 c         5814 g         4856 t
ORIGIN      
        1 cggcgcgcgc ggcgtggcgc gcatccatgg aggcttttca tgcccggccg gtggaagcgg
       61 atttgtttcc gggaagcgac attccgcgcg aagccccgca ggccgacacc tccgccatcc
      121 cgcgttatgc cataaccgct gaagatcttg ccgcaggtct tttgatttcc tctttgtgcg
      181 cgaaagccgg gctgacgcag tccatgtcgg aggccaagcg tctgatcggg cagggcggga
      241 tctatgtcgg agagcgccag gtgaaatctc cggatgaaaa gttgtcgcct gacgactttg
      301 gcggcggccg agaactgcgc atccgcaaag gcaagaagaa gtatctgatg gtggaaatag
      361 gggattaggc tgatccccat tgcgggggac gcgcgaccga aggctgaagg ctgaagaata
      421 gaggcgaaag gctggaggcc ataggtctaa gtggagtccc cgctccttcc tttgtggcga
      481 tgattagacc gatctccctg cgggagcctg cgacttaatc atcatgattt tctgaatcaa
      541 tggattgtaa aagaataatt ctatgttaaa atgcatcgca aaaggatgct catggaattt
      601 aagaccgttt tggaaaaact tctgacaaat ttcgaaagac tgaacattcg ctacgcgctg
      661 atgggcggtt ttgcgctggg attatggggg tacggacgcg caacggctga tgttgatttt
      721 ttggcgcatc gcgatgatat ggataaagtc gatgccgtca tgaagagtct gggctacgaa
      781 tgcctgtata aaagcgacaa tgtttctcaa tatctgtctg acctgaaaat attcggcgaa
      841 gttgatttta ttcacgcctt cagagcgaca agtcttgcga tgttggagac cgccgaagaa
      901 aaaatggttt ttaacggtga tttgaaaatt cgtgtattga aacccgagag tctgattgct
      961 tttaaattac aggcggtaaa aaacaatcct tcaaggacat ccgtcgattt gcaggatatt
     1021 aaaaagctgg ctgaaatcaa caaacgggac atggattgcg gtcttatcga tcgctacgcc
     1081 gaattgctga atatggaaga ggtgtggcat gagatcaaaa aagaggtgtt tccatgaacc
     1141 cggctcccgg cggtgaagat atgctcgacc aaacgattac ggaagatatc aaagccatgg
     1201 agagtcggcg gcataatccg tttatgaaaa acggcaaagc ggacgttgat gcctatatcc
     1261 ggttcgtttg cggcttcaac gaattcatca accacgatcc aaagccattt tcccaaatga
     1321 tggatcggga catgattcta taatcccagg tttcaaacct ttatccccca tgcaacctga
     1381 tttcgccata tgattccgaa ggagacttct aatgctcaag gaagggcagg ggtttttcca
     1441 tccggtacac cagcgcctgg caggccgcca gaagatttcc cgattcgtcc gtggcccgga
     1501 tatcgtaggc ggcggttcgt tttgttttat tgatttcccg ggcttcggcg ttcacgagcg
     1561 atccttttgg gggtgaggcc agatactgga tgctcatact gagggcgacg gccatcgtgc
     1621 cgtgcgagtt ggaggctgct tcaaaagccg cgtccatcag cgagaaaatc gcgccgccgt
     1681 gcgccatgcc gaacatgttt tccatgtcgg acgtaaagcg catttccacc cgggcgtggc
     1741 cttctttcaa atcaaccagc gtgatgccga atttcctgcc gaaaggctcc tgctccatcc
     1801 gttgaaagat tgcttttctg attttttcat ccattcctga tctccagggg gcattttccg
     1861 gatcggccgg ttttgctgtt tcctttaaca ggttggcggg caaagagcaa ccccgccgcc
     1921 ttgaaagtca acgtccggga gcgggaaatg agattttccg gcgggtcccg ccgcgttcgt
     1981 ccgtctccgt caaagaaaac gcccggtttg aggccgggcg ctttttgctt tggatgcttt
     2041 ctcgcgtgga ctacagaatt ctgacgccga taatcccgtt gacggcctca aatttcttga
     2101 ccagttcggc tgcgttgatg tcgccctcca catcgataat attataggcg atattgcccc
     2161 ggctcttatt gatcatatcg gcaatgttga gcttgttgtc ggccagcagg tgcgtgatct
     2221 tttcgatcat gccgggctca ttcttgttgg aaaccgtgat gcgggttttg tcggaccggt
     2281 cgaggatgca ttccggaaag ttgaccgagt tcttgatgtt tccgttttcc aggaaatcca
     2341 tgatctgcat ggtggccata atggcgcagt tttcttcgga ttcctcagtg gacgcgccca
     2401 gatgcgggat ggcgatgacc ttgtccgtct tgataatctc ttcatccggg aaatccgtca
     2461 cgtagcggct cacgatcccg tcggcaatgg cctttttcag ggctgcggtt tccacaagcc
     2521 cgcctctggc gaagttcagt aaaacgacgc cttttttcat gatggcaaac ttgtcggcgt
     2581 tgatgaagcc cttggtgtcc ttgttctggg gaacgtgcag cgtgatgtag tcggattcag
     2641 caagcagtcg atccaggctc ggcgccttct tgatgttgcg ggacagcttc caggccgcct
     2701 cgatggacaa aaacgggtcg tagcccatga cgtccatgcc cagagcctcg gccgtgttgg
     2761 cgatcatggt gccgatggcg cccagaccca ccacgccgag ttttttgccc aggatttcgg
     2821 ttccgacaaa ctgggctttt cctttttcga tcaggtcggg caccttgtcg ccttcaccca
     2881 ccagcccttt ggcccagatc aggccttccg ccacgttgcg cgccgcaagc agcatgccca
     2941 aaacgaccag ttccttcacg ccgttggcgt tggcgccggg ggtgttgaac accacgatgc
     3001 ctttctcgga gcatttatcg atgggaatat tgttaacgcc tgccccggcg cgggcaatgg
     3061 ccttgagact ggacggcagc gtcatctctt tcatttcctt gctgcggacg agaatgccgt
     3121 ccggatcggg aatatccgtg cggtattcat acttgtcggt gaaaaggctc aacccctttt
     3181 ccgaaatttt attcaacaga tcgatccggt acataaatgc ctcctacact tttgtttttt
     3241 ggtgggccgt gcggggattg aacccgcggc caacggatta aaagtccgct gctctaccgc
     3301 tgagctaacg gccccacctg aagcgttttt aaaagaacaa ggctctctag caactcatcg
     3361 ggggcctgtc aagaagaaac tggacgtttt caattttcaa aaggattttc cagttgaatg
     3421 gtctcgtcgc ggcccgcgcc tacggaaacc agagcgatgc cggcgccgga taattctttc
     3481 aggcgctgga tgtatttccg agcgtttacg gggagttcgc tgatgttctg cgcgcttgcg
     3541 atgttttccg tccagccgtc gaattcctcg tacaccggtt cgcaggttga aaagcgtttc
     3601 aggctggcgg ggaccgcttc gcggcattcg cccccggcgg ttttataacc gacgcagatt
     3661 ttgattttgg gaagccccgt caaaacatcc agtttggtaa tggccagcgt ggaaatgccg
     3721 gagacccgga cggcctggcg caccagaacc atgtccagcc agccgcaccg ccttttgcgg
     3781 ccggtggtcg cgccgaattc ctgaccgatt ttctgcagat gatctccgat tgcgtctttc
     3841 agctccgtcg ggaaaggtcc ttctcccacc cgcgtcgtat aggctttgca gattcccacc
     3901 accttcgaaa tggcgccggg gccgacgccg cttccgcagg cggcgtttcc ggaaacggtg
     3961 ttggaagagg tgacgtaggg atacgtgccg tgatcgacat ccagatgaca tccctgggcg
     4021 ccttcaaaca ggatcttttt ccccttttga atttctccgg ccagaatcag ggacgtatcg
     4081 gcgacatacg gcctgatttt ttccgcgtac gccaggtact catccgcgac ggcctgggga
     4141 tccagcggcg ccacgccgaa aaaattcttg aacaggtagt ttttttcttc caggctgtgg
     4201 cgcacttttt ccaggagcag cccgggttca tagaggtcgc ccacgcggat gcccgtgcgg
     4261 gcaaccttgt cttcgtatgc cgggccgatg ccgcggccgg tggttccgat tttcttgccg
     4321 gtattgcggg cctcgcgcgc ctgatccagg ctgcggtgat agggcatgat gacgtgcgtc
     4381 ttttcgctga cgtagagctt ggtggaagag gggaatagcc ctccctcgtt caattccttc
     4441 agctcctgaa ggaaaacctt cggatcgaag accaccccgt tgccgatgat gcagatttta
     4501 tgatcgtgca gaatgccgga gggaatcagg tggagaatgg tggtcctgcc tttcaccacg
     4561 agggtatgcc cggcgttgtt gccgccttgg aaccgggcga ccacatccgc tttgagcgta
     4621 tagagatcga caatctttcc ttttccttca tccccccact gggcgccgac aactgcaaca
     4681 ttggccatga gattttttct ttcctacatc ttgatcggtg tgatggaaac gacatgcggc
     4741 aatttccgca gtttgtccag aatgtccggc ccgaccgggg aatccgtgtt caggacgacc
     4801 agcgcttttt tcgactgcgc atcccggctc agatgcagac gggcaatatt gacgttatga
     4861 ctgcccagcg tggttcccag gttgccgatg acgccaggca cgtctttgtt ggagaccgcc
     4921 agcaggtaac cctccggggc gatttccacg gaaaattcat tgatccggac gattctcgga
     4981 tcgttttggc cgaaaaccgc gcccgacgtc cggctggttt ccttggaggt ccggacggtc
     5041 agggcaatca ggttcgtata gtctttggtt tccgaacttt tcacttcaat gatccgaatg
     5101 cctctttctt tggcgaggac cggcgcgttg atgtaattga cggtttcggt cagaatctgg
     5161 tccagaagcc ccttgatcag agacacggtg atcggggcga cattatggtt gaggatttcc
     5221 ccgctgtatt ccagcgaaac ttcctgaata gcgcccgtca gaagctgggc ctgaaaggcg
     5281 cccagcttct ccgccagatc caggtaggga cgaattccgg ccatcacgtc cgcgctcacc
     5341 gacgggaaat tgaccgcacc cttgatttca ccggtggtga agtaggcggc gatctggtgg
     5401 gcgatggcga tggccacgtt ggtctgcgct tcatcggtgg atgcacccag atgcggtgtg
     5461 cagattacat tgtccagacc gaccagctcc atgtttttgg tcggctcttc ttcgaatacg
     5521 tcaatggcgg cgccggcgac ctggccggct ttcaaagcgc ggcagagcgc tttttcgttg
     5581 acgatgccgc cgcgggcgca gtgaatcaga aacacgccct ttttcatttt tgcaagcgcc
     5641 gattcgttga tcagattccg cgtttctttg gagagcgggc agtgcacgga gatgaagtcg
     5701 gaattggtga acagttcgtc caggttcacc atgttcacgc ccatctgttg cgcgacttcc
     5761 ggagaaataa acggatcatg ggcgatgacg ttcatcttca ggccgatggc gcggtcggcc
     5821 accacggcgc cgaccctgcc caggccgatg acgcccaggg ttttattgca gtattcattg
     5881 cccatgaatc tgcttttttc ccagcggccc gctttcatcg aggcggtggc ctgtggaatc
     5941 ctgcgcgcga gcgcaagcat catggcgatg gcgtgctccg cggtggtgac ggtgttgccg
     6001 ccgggcgtgt tcatcacaac gatgccgcgt ttgctggctg cggggatgtc gatgttatcc
     6061 acccctgcgc cggcgcggcc gatggcggaa agctttgcag ccgcctcgat gatttcggcc
     6121 gtgactttgg ttgatgaacg aacgatcagg gcgtcgtagt ctttgatgac cgcctttaac
     6181 tcatcgggct tcatgccggt cttgacatcc accttgatgc ccggcgtgtt gcccagaatg
     6241 gccgcgactt caggcgccat ggaatcgctg actaatactc gtttcatagc gtttcccgca
     6301 ggagccttcc gctacaggct cttgactttt tcgttgaaaa ctttttccag ttcagcaagt
     6361 gcgattttga gatccgcttc ttcaatggtc gggccgcacc agaaacgata gccggcgggc
     6421 gcgtctttgt aagagttgat gtcgtgcgca atgtttttct tgctcaggtc cgaagcgatg
     6481 ctctttaaga acttgctttt ggcgtctgcg tccatcgcgg ccactttggg atgcgtgacg
     6541 gacaggcaca cggaggtgtt ggagcgggtt ttcgaatccg ttgccaaaaa ttcgatccag
     6601 tcggttttgg cgacccagtc ttcaacgact ttcaggttgg ctttgctctt tttgatcagg
     6661 ccgtcgatgc cgaaggtgcc ggcccattcc agagcgtcga tatagtcttc cacgcagagc
     6721 atggaggggg tattgatgac atcgccttcg aagatcgcct tgttgatctt gtcgcctttt
     6781 ttcagacgga agattttcgg catgggccag ggcggtgtgt aggattccag acgggcaact
     6841 gccctggggc tcagaatcat catgccgtgc gccgcctcac cgcccaggca tttctgccac
     6901 gaatacgtca aaacgtcgat cttgctccaa tccacctcgt tggcaaaggt gtaactggtg
     6961 gcgtcgcaaa gcgacaggcc ttcacggtcg gcgggaatcc agttccagtc gggaatgcga
     7021 acaccgctgg tggtgccgtt ggcgacgaaa accacatcac tcttccaatc gactttggac
     7081 aggtcgggga ttttgccgta gtcggctttg agaatcgtgg ggttcaattt gagctgttta
     7141 ttgacatccg tggcccagcc ttcggagaag ctttcccaga ctaaaaccgt aaccggcctg
     7201 ggaccgagaa gcgaccacat ggcgcactca aaagcgcccg tatcggaagc cggcagaatg
     7261 cccacgaggt aatctgacgg aatgtttaat actttgcggg tatcgttcat cgctttaacg
     7321 attctttctt ttcccagtgc ggaacggtgg gagcggccga ccggcgcgct cttcagcgcg
     7381 tcgatattcc acgctggcct cttgctgcag ggaccggaac taaagtttgg gtttaacatg
     7441 ggtaaccatc cttaaaataa attttcttaa tgctggcatg ttcataccag cattaagcgc
     7501 ttttctcaag aaatttaaac cgtaatagac ctcccgtgac tcatggtcga agcggctgcg
     7561 gctgaaactt tcgaggcgtc agtaaaataa caatttttga cttaagtcaa atacaatgtt
     7621 gttcaggtgt gctataaaga cacaaaatag gatgtctggc ttcagaggca ttaaaaaaga
     7681 agggggaaaa aatgactgaa aatctggcca aaatcgcgga tctcactggc gcttttcgtc
     7741 ttttggagct ggttgactat caggggcgct ccgtggtcag ccgggaaatt atccgcaaac
     7801 ctttcggaac catgacggtt tttgcattcg atgccggcga gggattaagc gagcacacgg
     7861 cgccttttga cgcgctggtc tatctgctgg aaggcaaagc ggaaattacc attggaggaa
     7921 agcctcacgc ggtcggggag ggggagatga tccttatgcc ggcgcacaag ccgcatgcgc
     7981 ttaaggccgt tacccgtttc aagatgctgc tggtcatgat taaataaaaa aacaggaggt
     8041 gttttatgaa attcgagatt acaccgggtg tggattgggt agggaagatt gactgggagc
     8101 tgcgcaagtt tcacggagag gaatactcga cgcatcgcgg ctcgtcgtac aacgcatatc
     8161 tggtgagaga tgaaaagacc gctctcattg acacggtctg gtcgcctttc gcaaaagaat
     8221 tcgtggacaa tcttgccaaa gaaatcgacc tcaacaaaat cgactacgtg attgccaacc
     8281 acgcggaaat ggaccactcc ggcgccctgc cgctcctgat gcaccggatt cccggtaagc
     8341 ccgtgtactg cacagccaac ggactgaaat ccctcaaggg ccactatcac aaggactgga
     8401 acttccaggt ggtgaaaacc ggcgacaagc tcagcctggg cggaaaggaa ctgattttcg
     8461 tcgaagccat gatgctgcac tggccggatt ccatgatgga gtacctcacc ggcgacgcga
     8521 tcctgttttc caacgacgcc ttcggccagc atattgccac cgagcacatg tttaacgatc
     8581 tggtcgaacc ctccgaactt tttgaagaag cgaccaagta ttacgccaac attctcacgc
     8641 cgttttcgcc gctggtgacg aagaagatca acgaagtggt ggggctcaat ctgccggtga
     8701 acttcatctg tcccagccac ggcgtcatct ggcgcgacaa tccgctgcag atcgcgcgga
     8761 aatacctgca atgggcggcg gactatcagg aaaaccagat ctcgatcatt tacgacacga
     8821 tgtgggacgg aacgagaatg ctggccgagc acatcgcccg cggaatcaaa caggccgacg
     8881 acaaggtgac agtgaagctt ttcaatatct cccgccgtga catcaatgac gtggtgaccg
     8941 aggtctttaa gtccaaggcg attcttctgg gatcgcccac aatcaacaaa ggcattctca
     9001 acgccatgtc ggcgctcatc gaaatgattg cggggctcaa attcaagaaa aagaaggcaa
     9061 cggccttcgg ctgctacggc tggagcggcg agagcgtgaa gattttaacc gatcatctgt
     9121 ccaaagccgg ctttgaaatg atcggcgacg gcttcaaagc catgtggcag ccgggggatg
     9181 aagcgctcgc gcaggcgctg gcgtttggaa aggattttgc ggggaaagta taatttttgc
     9241 taaacaaaat caaaaaaagg gtggaagccg ctttgcgttc cgcccttttt ttatggatgc
     9301 tggatcgttt tacgcttatt tgatgcagac ccggcggacc tggttgaaat ccgtaatgcc
     9361 ctgcagcact ttctggattc cgtcctgaag cagcgtggtc atcccctcgg ccatggattt
     9421 ggcgcggatg acctcaaccg tttcatgctt ttgcaccagt ttcttgatgg cgtcggtggc
     9481 cacgatcagt tcatgaatgc ccattcggcc cttgtagccg gttttgtcgc aggcgtcgca
     9541 tcctttggcg cgatacagct tgaaatcatc cgtgtaggaa acgcccagtt tggcgaagtc
     9601 ttcgcggcca taactttcca cgatttcctc gaattcctcc tcggtggcgt gatactgttc
     9661 tttgcagttc ttgcacagcg tgcgcaccag gcgctgggcg agaatgccca acagggcgtc
     9721 cgcgaagttc agcgggtcga tgcccatatc caggagccgg acgatggttt ccggggcgct
     9781 gttggtgtgc agggtgctga agaccagatg gccggtcagc gaggcttcaa cgcctgtctt
     9841 ggccgtttca aaatcgcgca tttcacccac catgatcaca tccgggtcgg cgcgcagaaa
     9901 cgcccgcatg gcggccgcga agtcgaagcc gattttgggc tgcacctgaa cctggcgaag
     9961 cccgtattgc gtgatttcca ccgggtcttc agcggtccag attttccggt ccggccggtt
    10021 gatgacgtgg agcgccgcgt gaagcgtggt ggttttgccg gagcccgtcg gcccgacgac
    10081 caggatcatg ccgtagggtt tgttgatgat atcgatcatg ccgtcataat tgcgcttcag
    10141 cagccccatg gattccagcg gcaggagcgt gtcgctcttg gccagcaggc gcatgacaat
    10201 gtcttccacg ccgccctgcg tggggatggt ggcgacgcgc agctccagtt cgtcgccgga
    10261 agggcgccgg aacttgattt tgccgtcctg aggctttctt ttttccgtaa tatccagatt
    10321 ggacatgatc ttgacgcgcg agatgatcgc agaccggtag ctgaagggga cggtctggta
    10381 aatcgtgcag tcgccgtcaa tgcggtaccg gatttccaca tttttcttgc cgacgttggg
    10441 ttcgatgtga atgtcggacg cccggcggga ggaagcgtcg ttgataatct tgttgacgag
    10501 ctgcatgatc acgctgtcgg tttccgcgac gacgtccacg ccctcttctt cttcggcgcc
    10561 tccgccttct tcatccatct tgcccaggat ttccgtgaag gaatgttcgt cttcgccgga
    10621 gctgaagaaa aggttgatga attttaagat atcctccggc agcgcgacgt catacttcac
    10681 cttcttggtt ttcagaagat tttcgatggc gtctctttta atgatgttgt tcggatcatc
    10741 gacaatcacg tggatgttgc cgtcgatttt ttccagcggc acccacagct cccgctggag
    10801 gtagtctttt ttcaggttgg caatcaagtc gccggggatg atgatttttt cgttgtaatg
    10861 aataaaccgg cagcggtaaa actcttcaaa ggagtggccg atgtcgtctt tggagatttt
    10921 gtatttgttc atcagatacc gctcgacgga ttctttctgc tccctcgctt ctccccaggc
    10981 ggcttccagc tcatcttcct tgagcaggtt gcgggtcagc agaaaatcga aacgtgtctt
    11041 gcggcggcgc tggaagcgct gctgattgta gaaggcaacg cccaaaacct ccacgatttc
    11101 catcaccaga ccgatgtctt cctcggtgaa tttgctgtct ccccgttttt tgttgaggat
    11161 ttgaatgacg cccatgagct gatctttata aaaaatcggg gcggccagaa tctgcttggt
    11221 cttgaaaccg gactttttgt cccagctggc gtcaaaggtc agttcgggat cgatggcctt
    11281 gagctccgcc aggttgtatg cgttcgcgat gttgacgata ttgaaggtgt tggccacgta
    11341 tccggcgatg ctgtgattgt tgatcggaac gcggatctct ttgagctggg cccctgccag
    11401 aaacatggaa tagatttcat tgtgcggttt atcgacgaca tagagcgtga tggagtaggc
    11461 gtcaaacaga ttcaggatgc cgtccttcag atcgatcagg atttgggcaa tattctgcgc
    11521 ggcatgaatg cggttggtga tgtcctgaag ttttttctgt aagcgcagcc tttcttccag
    11581 actcacttct atggggggct tcgtcttcat gatcatccgg ttatctctcc attttcgttt
    11641 ttctcataaa aaccgcgcac atcctagcaa gccctgcagg gaatgtcaat caaacgaatt
    11701 atatccaatg cgccggaaaa ggctgcggcg cggcgccgcg cggtttagcc ggcggtgacg
    11761 attttcgaga aatcggcgat ttgatggaac agcgccgaga tgtccgccag cagggacagg
    11821 cggttgaatc ggattttttc atctttgtcc atgaccatga cggaatcgaa aaaagcgtcc
    11881 acgggggagc gcagggcggc cagtttgttc agcgccgcgg tgaaatcttt ctcagcgatg
    11941 cctttttcca cgcgcgtgcc gatgttttca aaattcgcga acaacttgat ctccgcttcc
    12001 tgggaaagca ggttaacgtc aacggaaccg ccgtggaaat ccttcaggat gttgtcgatc
    12061 cgcttgaaag cgatagagag cggctcgaac tccggattgg cgcggaaggc ctgcagcgcc
    12121 tgaatctttt cgatcaccaa aacgagccgg tcgatgtccg cggccagaac cgcgtccacc
    12181 gtatcgtagg cgtagccctg gctgatcaac tgattctgca gacgcccccg gaagaattcc
    12241 aggacgtcct ttttgacgtc ttcagccggt ttcctcaaaa catccttcag ggacgccagg
    12301 cattcgtcga tcagaaaatt caggctcaag tcgtagcggc gcgacaggat gatgttgatg
    12361 acgccaagcg cctgccgccg gagggcatag ggatcggccg tgccggaagg cggcatgccc
    12421 acggcaaaga agccgacgat ggtgtccatc ttatcggcga tgccgacgat cgcgccttca
    12481 tccgtgccag gcagatcgcc tccggcgaca atcggcaggt aatgctcata aatggcatcg
    12541 gcgatttcct gtttttcccc ggccaggagc gcgtattccc gccccatgat gccctggagt
    12601 tcggaaaatt cgcccaccat gagcgattcc agatcggcct tggccagaag tgcggcgcgg
    12661 tccacgtttt tcttgactgc gggctttacc ttggcggcaa ttttgaccgc cagcttgcgg
    12721 aagcgcatca ccttcttatg ggaggttccc agcagcgtgt ggaagacgac tttcttgagc
    12781 gattctaccc ggtcttccag cgggactttt ttatcctctt caaagaagaa cgaagcgtcg
    12841 gccagacggg cctgcagcac acgttcgttg ccccgggcga ccacttccag atcccgggcg
    12901 cgcgtgttgc tgacggcgac gaaatagggc agaagctttc cggcgtcgtc cacgatgggg
    12961 aaatacttct ggtgcgagat catggttgtg gtcagcacgt ccttggggat ctgcaggtaa
    13021 tgggggtcga agccgccgcg aacgatcacg ggatattcca cgataaacgt gatggtttcg
    13081 agcaggtctt ccgtatagaa aagctttccc ccgacatcgg cggccgattt ttgcgcttct
    13141 tcgagaatca gttttttccg ctcggcggga tcggcgatca cgaaacgctc ttgggctttg
    13201 gcgaggtaat cttcataccc ctcaacggca aaggcctccg ggctcataaa gcggtggcct
    13261 cgcgaggtgt tgccgctttc aatgtcttcc accttgagcg gcacgacctt gccgccataa
    13321 agcgccagca gccagtggat cggccgggca aagcgcagat cgtaggcagc ccagcgcatg
    13381 gatttgcgaa acggaatcgc cagaagaaag cttttgagga ggtccggcag caggtcggcg
    13441 gtcggctgtc cggcaatcgt cttgcgcgca cacagatatt cgcccttgtc ggtcgtgacc
    13501 gtttcgagct gcgagacttc cagtccctgt ccccgggcaa atcccagcgc agctttggtc
    13561 ggctggccgt tttcatcaaa ggccgctttt ttggccggtc ccattttttc gacgacctgg
    13621 tcgtcctgct tcggcgcgag atccgcaatg tgcagcacca gccgtcgggg ggtggccagg
    13681 cagcggatgc cctcaaaggc aattcgcttt tccgaaagcg ctttgcggat catttcctcc
    13741 atatctacca ccgctttgga taaaaacccc gcgggaattt cttcggtgcc gatttcaaaa
    13801 agaagttcgt tgctcatcgt ttctccagag gttctattgg ttctgtaaaa gagggtatcc
    13861 cagctcttcg cgctgcttca tgtaaagttc cgcgctgatc ctggccaggt ttcgcacccg
    13921 gccgatgtag ctggtgcgtt cggcgacgct gatcgcgccg cgagcgttga gcagattgaa
    13981 ggtgtgggag catttcagac agtagtcata ggcgggcagc gacagatttt tttcggcaat
    14041 gcgcagcgcc tcctcttcgt acatgtcgaa gagtttacgc agcatcggaa tgtcggccac
    14101 ttcaaaatta taggtggaaa attcgacttc gcccttgtgg tgcacgtcgc cgtacttgat
    14161 gttgtccgtc cattgcagat catagacgtt gttgatgccc tgaatgtaca tggcgatgcg
    14221 ttccgttccg taggtcagct cggcgcagac cggcttgcag tcaaagccgc ccacctgctg
    14281 gaaatacgtg aactgcgaga tttccatgcc gtcgagccac acctcccagc ccagtcccca
    14341 ggcgcccagc gtcggggatt cccagtcgtc ttccacaaaa cggatgtcgt gctccagcgg
    14401 atcgatgccg aagcttttga gagaatcgag gtagagttcc tggatattga ccggcgacgg
    14461 tttcatgatt acctggtact ggtaataatg ctgcagccgg ttggggtttt cgccgtagcg
    14521 tccgtcggtc ggccgacgtg aaggctggac gtaggccacg ttccatggtt ccggacccag
    14581 cgcccgcagg caggtggcgg gatgaaacgt tcccgcgccg acctccatat catagggctg
    14641 ctggatgacg cagccctgag aatgccagta ttgttccagc gcaaagatta attcctgaaa
    14701 agtcacaatg tcctccttgg ggatgccggc aggaaccctg accggtcgcg cgggtccggg
    14761 gctttacctc tgccagcggc tacctagcat gaaagaccaa aggggtcaag attgagtgat
    14821 gggcaaaggg cgaggggcga ggggcgaggg gcaagaaacg gtcgcgaccg ttccttgcgg
    14881 ggcgcaggtc atgggaaaat gctgaattcg cggttcgcgc aaaagatgcg gcatgaaaat
    14941 gtagtgcggg ggcagccccc gctatgtgcg cgcctaaagc cccgcactac aacgttttgt
    15001 cctggtgagc ctgtcgaagc atgatgatgt cgccctccga caggagacct ttgaacaact
    15061 ccattttacg ccattctcac gaaggcggga atccaggaat tatttgataa tactggatgc
    15121 cggcctactc cggtatgaca aatgttgcca aataccttca gcggggtcat cagcgccgca
    15181 tctcatatct tgtggcaaaa atcttcttga catcctacaa ggcatctttt acactccacg
    15241 ctcacaaaca ggaagttttt accaattacc aatttatcaa tccaaaggag ggcgagtttt
    15301 atgacaggaa aggtatacga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 ttaccaattt atcaatccaa aggagggcga gttttatgac aggaaaggta tacgaacggg
    15481 gggaatgcta caagtaccaa ttgctgatca aacatctgct ggagacgccg ttgttttacg
    15541 ctcccgagca ggaaatcgtc tatcgggaca aggtccggct gacctatcga cagctcaacg
    15601 agcggattca ccgtctggcc aacggcctgg agaaactcgg cgtcaagaag ggcgacaccg
    15661 tctgcgtgtt cgactatgac agccaccgct tcctggagtg tttcttcgcc attcccatga
    15721 tgggggccgt tctgcacacg caaaactggc ggttgtcccc ggagcagatc ctctatacga
    15781 tgaaccacgc cggcgatgac gtcgtgatca tcaactcgga ttttctcccc ctgctcgaag
    15841 cggtgaagga caagctcaag acggtcaaga agatcatcct gatcgatgag gatgggacca
    15901 agccggccac ggcgctcaag atcgacgtcg agtacgaggc gatgctggcc gcctcggcaa
    15961 ccttctacga ttttcccgag ttcgacgaac agacgcgggc gacgatcttc tataccaccg
    16021 ggacgaccgg tgatccgaag ggcgtctatt tttcccatcg ccagatcgtc ctccacgccc
    16081 aggccctgct cctggtcacg agcgcctatg acggaatcgg ccggttcaac tcgagcgacg
    16141 tctacatgcc catcaccccc atgttccacg tccatgcgtg gggggttccc ttcgcggcga
    16201 cgatgctggg catcaaacag gtctaccccg gcaagtacga gccggcgatg ctgctcaagc
    16261 tgattttgac ggagaaggtt acgttttcgc attgcgtgcc gacgatcatc cagatgctcg
    16321 tcagcacccc ggtggtcaag cagttcgacc tgacgaaatg gaaggttatt atcggcggcg
    16381 cccgtctgcc gaaggggctg gcccaggcgg cgatggagat tgggatccag atctacgcgg
    16441 cctacggcat gtccgagacc tgccccctcg tttcgatggc gaacctcaag ccggacaagg
    16501 tcggcaaggg catgatcgag aacgtcgaca ccctgattcg taccgggttg ccggcgccgc
    16561 tggtctatgc gcgcatcatg ggcccggacg ggaaactcct gccgcacgac ggcgagacga
    16621 cgggggaact ggtgttgcgc gccccctggc tcaccgaagg atactacaaa gagccggaga
    16681 aatccaagga cctctggccg gacggctgga tgcacaccgg cgatgtggcc tacatcgatc
    16741 cggagggtta catccagatc accgatcgga tcaaggacgt catcaagacg ggcggcgaat
    16801 gggtttcctc gctggagctc gaaaatctca tgagcctcca cgaagccgtt gccgaggcgg
    16861 cggccatcgg cgtgcccgac gccaaatggg gcgagcggcc catgctgatc ctgacgctga
    16921 agcccgactt caagggaaag attaccgagg aggatttcaa gcgatacatg atggaggcct
    16981 cgacggccgg gaagatcccg aaatacggcg ttccggaccg gtacgtgatg gtcgaccaaa
    17041 tccccaaaac atccgtgggg aagatcgaca agatcaccct gcggaagcaa tttcaatagt
    17101 tttcaggggg cgtttctcag agtcgagaac gtccccgcgc caacctccat atcccagggc
    17161 tgccggatga cgcagccctg ggaatgccgg tatttttccg gcgaaaaaat gacggcttcg
    17221 taaaaagccc ggatgccgcg ttgcgccgca tccttccccg gccttcaccc ccggcaggct
    17281 tattgcggcg cccgctgaag tacgcctcat tgctcaggat ttgcgcgcct tgcctgcggg
    17341 gcttcaaacc ctctaaaacc tccctgtgct ttgccctgtt cctggcgtgg acagatattt
    17401 ttctgttgac atgccgggga gtcttttcta tgaacaagtt cattgattgc gggtgtttgt
    17461 caaaggcgtt tctttaatcc ggtggagatg aatatgctca attttacttt tcaaaatccc
    17521 acgaaggtga ttttcggccg ggatgcgata tcgaagatcg ccggtgaggt tccacgggat
    17581 gccaggttga tggtggcctt tggcgctcaa agcgcccgga aatacggcgt cctggatgaa
    17641 gttctcaggg cgctggcagg ctttgacgtt accgaatttg gcggcatcgg gccgaatccg
    17701 gaatatgaaa cgctttgctc cggcattgct ctggcccgcg aaaaaaagat cgatttcctt
    17761 ctcgccgtcg gaggcggttc cgtgattgac ggaaccaagt ttatggccgc tgccattccc
    17821 ttcagcggcg agccttatct gctcatggaa aacaaaggcg ccgccatccg ccgggcaatg
    17881 ccgctgggcg cggttctgac attgccggcg agcggttcgg aaatgaacaa ccgggccatt
    17941 atttcgcgcc gggccgcaaa ctggaagcgc gccatcatga acgactttct gtttccccgt
    18001 tttgccgttc tggatccgac gaaaacgttc acgctgccgc cggccgaaac cggcaacggc
    18061 gtggtggata catttgttca cgtcctggag caatacctga cgtatcccgt ggacggcaag
    18121 gttcaagacc ggcttgccga agggctgctg ctcgcgctga tcgaggaggg gcccaaagcg
    18181 ctccgggagc cggaaaatta cgatgtgcgc gccaacctga tgtgggcctc gacgctgggg
    18241 ctcaacggtt tgatcggcgc aggcgttccg caggactggg cggcgcaccg ggcaggctac
    18301 gaactgacgg ttttatacgg actggcccat gcgcagacgc tggcggtgct ggtgccggcc
    18361 atgctgaaag tccggtccac aggcaaaaaa caaaaactgc ttcagtacgg cgagcgcgtc
    18421 tggggaattt catccggcgc ggagacgctg cggatcgacg cagcgattga gcggacgcga
    18481 acttttttcg agacgatggg gcttcccacc cgctttgcgg actgcggcat aaaaaatgtc
    18541 gatgccgaca gagtggttga actcctcgcg gaacatggtc tgatcacgat gggcgaaagc
    18601 ggcgacgtga ctcccggcac cattcgggag atcctggcgc tgtgttgatt tttgaccgaa
    18661 ccggtctgcc atcccgcttg cggcagagag atggattgac actgacccac tgaaaaggat
    18721 tttcatcatg cacagtattt tacctgaaat cggcattgcc gttctggcgg ccacggcaat
    18781 gggatttatc tttcaactct gccgtcagcc ggtcatcctc ggctatctgg tggccggagc
    18841 cctgatcgga ccgcaaatcg gtttcaagct cgtctccgat ccggccaata tcgaagttat
    18901 ctccgagatc gggctgatcc ttctcttgtt catcatcggt ctggagctca atcccgccaa
    18961 acttctttcc tccgggaaaa aactgatcta tgccggcgtc ggccagtttg tcctgtgcgt
    19021 tctgatcggc ctgggcttct ttgttctgct gggctatccc ctggggggcg gacgcattga
    19081 agcgctttac ctgtcgctgt tctgcgcgct ttccagcacg gccattgtcg tgaagctcct
    19141 gtatgacaag ttcgagctgg acacgctgcc cggccggatc agtctgggca ttctcatttt
    19201 tcaggatttg tgggccatcc tcattcttgc gctccagccg aatttcatgg acccgaagct
    19261 gcacctggtt gccctggcgc tgggcaaagg cgtcctgctt ttggccgtcg ggtttttgct
    19321 cagccggttc gcgctgcgct ggattttcgc aaaaatcgcc aaaactccgg aaatggtggt
    19381 tgccacatcc atcgcctggt gcgcctttat ggccggactg ggctcctgga tcggcatgtc
    19441 catggaaatg ggcgccctga tcgccggcgc ggcgattgct tctttcccct acggcgtgca
    19501 tgtcaccgcc aaggtcctgc cgctgcggga tttctttctg acgctctttt ttctttccat
    19561 cggcatgaag attccgtttc ccgacgccgc cacactgacc atggccgttc tcattgtcct
    19621 gttcgtgatc gcttcgcgtt tcctgaccat ctacccgatc ctgcagctgt ccggaagcgg
    19681 gcgccgcacc agtttcatca ccagcctgaa cctggcccag atcagcgaat tttccctggt
    19741 cgtcgcggct ctgggcgtgc aatacggaca tatccagcaa tctttgatgt ccctcatcat
    19801 ttacgcgatg gccatcacct ccgtgctgtc ctcctatttc atcaaaggca accacacgat
    19861 ctatcttgcc tttgaccggg tgctgacccg aatgggattt ccctccaaag ccgccaggga
    19921 aaccgcggaa gaagaacagg atcattaccc tatcgtgctt ctgggctatc atcgcggggc
    19981 gcgggccatc gttgaaaaga tccaagagga tgctcccgat ctgctggaaa aaattctcgt
    20041 catcgatttc aatctggaag ttcttaaaga gctggaagac cgccatgtca aaggggtttt
    20101 cggagacatc agcagtatgg acaccctgga ccacgcccac gtggccggcg ccgaagtcat
    20161 tttatccacg attcccgaca tgctgctgaa aaacacgagc aatctgtcgc tggtcaaaac
    20221 gtgccggatg ctggcgccca acgccctgat tgtggccacg gccgactccg ccgagcaggc
    20281 ggaagagctg aagaccagcg gagcgaatga ggttttgctt ccttactcgc tcatcggaga
    20341 ggccatcgcg gaaatgctgc tggattggca gcgcaaaggc gcctgagtag gggcgaaata
    20401 tttcgtggaa gttgttgaca tccgcatgga cttgtaatat gtagctcgct gaattttttc
    20461 cggcggcctt cctgcttgcg ccggggaaag aagttttgaa aataaaacat gttccccagt
    20521 agctcagtcg gtagagcggc tggctgttaa ccagcatgtc cgtggttcga gtccgcgctg
    20581 gggagccaa
//
LOCUS       DAOSRM010000032        20286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2819_length_20286_cov_118.426573, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000032 DAOSRM010000000
VERSION     DAOSRM010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 20286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20286
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2819_length_20286_cov_118.426573"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..848)
                     /locus_tag="PLA58_04760"
     CDS             complement(288..848)
                     /locus_tag="PLA58_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62137.1"
                     /translation="MNRKALFFVLIDVPFEVMPETRYENIRGQQVMGLKITWTSVIFF
                     FILSSLSVCSAEPPNPKIWEPLQYNSFYNKKIITKSPGILLVWTYRTITDEAREDRIE
                     EVKKYDPEKSIKYKAYHHETVLWEMNCANRRIRMEEIIDFDKNGKVLDRHQYDHPEWV
                     SVIPKSGGERLYQNVCVRLKTPSAKK"
     gene            complement(845..3760)
                     /locus_tag="PLA58_04765"
     CDS             complement(845..3760)
                     /locus_tag="PLA58_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOR62138.1"
                     /translation="MTKKLFLKMIFSLLLIAVITGLAPQNIFSQDRMSARGSRAIPVD
                     RPVAAIPGTDAGRASPVQAASTTAASAADKTWFGVAVQPVSGKDMRIMHMEYGRWPVV
                     IVSEVVNESPAEKMGIRLNDIILSVNYKDIKTPGEFQDAVAAISPGDKIVLLVAHRGE
                     KAQYLSGTVGHPTGATKTAAAAKPVTAESPSITAADIAAKIFAPTGSDTINAIRCSPD
                     GKYLISGGGYKKDALNLWDIAAGKKIRTYRGHTDAVNAVAFSPDGKIILSGSFDKTVK
                     CWDVAADKALWTFSGHEALILDVAFSPDGKYAASGSRDNTIRILDAASGKEIRTLSGH
                     TDGVKSIVFSPDGRFLLSGSFDKTLMLWDVQTGDRIQTFAGHQWQIASVAFSPDGRQV
                     FSRSGDRVVKIWDAATGREIRTFTADDVNAAGAKSGSQGIRAGAFSPDARRMLTSKSF
                     GGTLSLWDMDSGRILKKFAGHLGNVPAVDFSRHENYFLTGGQDGAIKLWDISAGREIA
                     QFVSLQNGEWIVLTPEGYYNSSLNGHQYLHIKVGSKVYGIDQFYDVFYRPDIVSAKLK
                     GEDISSLITLTIDDALKSPPPTVEFTALPQQTDSPKVRICYQATSAGGGIGEVRLFHN
                     GKLVQSDGYYREVAKIGAEPARLVSLDSGSIYANMRSVNIKARTDVSPISGKPKGDVF
                     EDCREIDAIAGENEVSVSAFNGGNTVQSYMKTAAFSSRIKAEEPHLYILSVGINQYKD
                     SSVNLRYAVKDAADLEGKIRAQSATLYQPQNIHVVLLTDAQASKAGITGKIQSLSTAV
                     QPQDSFILFVAGHGVLLQNQYYMLTHDFNGRMDEAGMISSNEIVEMSKKIKALSQLFI
                     FDTCHAGGVDTIISGLYDARMSVLARKMGLHIFASANDQQAAMDGYQGNGLFTYTLLD
                     GLNNNRQADKYKDGKVSIVGLGEYSQRMTSQISRQIGHEQTPLIINFGKDSPLYQLR"
     gene            4136..5524
                     /locus_tag="PLA58_04770"
     CDS             4136..5524
                     /locus_tag="PLA58_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR62139.1"
                     /translation="MRPIRDILEAQSVVVIGASRDQEKPGAQLLKVLKDVGFAGRVAG
                     VNPQGGNVFGVPLYPCVRDVPFEIDLAVLLIPPRFVPDALRDCAVKGVKGAVISAEGF
                     AETGTQGATYQEDVRAILKSSGMRGFGPNTLGLVNTQTKLTTSYFANSQMLASGHIGF
                     AAQSGIFVGALLRYLSSLTGLHLSKGIGLGNKVDVNESDALSFLADDEQTKIIGLYLE
                     DVKDGGRFVKNLKKATARKPVLIIKSGRTQAGAKASATHTASMAVEDSVFDGIVRQAG
                     AIRLHAIDEFVRTLKGFLNMPLPAGDRLAFVSYSGAQAIMSIDTTIEQGLQAARLADA
                     TQARIGRVIATSSKTKNPIDIFPDWLTHGYEKTITEVVKALLDDRGVDGIVFISFAEA
                     NPEVYQSLVNVVQADRRKPVFVSLLGDSLDKAACERLLDAGGLPCFDFPEQAVRVFAR
                     MRQYAKRIKGTE"
     gene            complement(5703..7538)
                     /locus_tag="PLA58_04775"
     CDS             complement(5703..7538)
                     /locus_tag="PLA58_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR62140.1"
                     /translation="MVEATAGKGASANLDAYYTKPNNLVDLFEESAARYASHNLFGTK
                     NKATNQYEWVTYSAVAERVNNLRGALNKLGLSKGEKVGVIVSNCTEWFVCANATHGLG
                     GVFVPMYEKELQKVWQYIISDAGIKYLFVSNQTIYDVVMGFKKDMPTLKDVFIIFGEG
                     EKSLAALEKTGKENPAPSYKPHWSETAFIIYTSGTTGDPKGVMLHHGNMTHNARECHE
                     TFDEGDPDEVGLCILPWAHSFGLTADLHTYILGGWALAFAESADKLIINFSEVKPTHM
                     SAVPRVFNIIYDKIQQGVAADPEKKKVFDAAVAEAVKNRDLKEKTEEFKFYDAVAFST
                     IRNIFGGRLKHVVTGGAMMKPEIALFFADVGVPTYDGYGLSETTPVISNNSPKRGNKY
                     GTVGKPFKDTHVVIDKSRVGEDSPDGEIVVYGAQVMQGYLNKPAVTKEAMMPDTWNGF
                     PGVRTGDRGWLDEDGYLHITGRFKDEYKLENGKYVHPESIENEMKLLRYISNAIVYGE
                     GRLFNVALVVPDFEALKADPKTSAWAQGSREETIANKECTDFISKEITEHLRKTFGGY
                     EIPQRFLYITEDFTVDNGMMTQTMKLIRRNIMKKYGDQLIALYSN"
     gene            complement(7710..9608)
                     /gene="dxs"
                     /locus_tag="PLA58_04780"
     CDS             complement(7710..9608)
                     /gene="dxs"
                     /locus_tag="PLA58_04780"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOR62141.1"
                     /translation="MKPPEIIPYSVLDSVNDPADLRSLSVAELQKLAGEIRDLIISTV
                     AATGGHLASSLGAVELTLALHYVFNTPRDRLIWDVGHQSYAHKIVTGRKEKFATLRQQ
                     GGLSGFPKRTESPYDTFNAGHSGTSIAAAAAFAEAQCLKGEHNKIIAVIGDGSLTTGM
                     AFEGLNWSGDRKKNLIIVLNDNEMSISPNVGALSSYLNRIMTGDRVTRFKSELKNFFK
                     SIPGIGEQILKFSQQIEESVKTFVVPGALFEELGFTYVGPLEGHRLDYLLKNFENVKK
                     LEGPVLVHVITQKGRGYKFAEENSPTYHGIAPFDVETGQTLPSVNPAPSYTEIFGRTM
                     TELAAADSRIVAVTAAMCEGTGLEKFRKAHPGRFYDVGIAEQLAVTFAAGLATEGLTP
                     VVAIYSTFLQRAYDQILHDVCLQNLPVVFAVDRAGIVGEDGATHQGLFDLSYLRNLPR
                     LVMMAPKDENELRHMLKTAVGCGSPVSIRYPRGKGVGVPLDSEITTLPLGRGEVLHEG
                     TDLAMMAVGSTVYPALQAARKLAAEGIGVKVINARFIKPLDAELLLGTAASVKKILTI
                     EENVLDGGFGSAVLELFAQNDVTGITVRRLGVRDEFVEHAKQSELRAQLGLDEEGILR
                     AAREMLQK"
     gene            complement(9625..10524)
                     /locus_tag="PLA58_04785"
     CDS             complement(9625..10524)
                     /locus_tag="PLA58_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOR62142.1"
                     /translation="MKHEDFLEAYLQDRCKIVEEALERYLPGKDNEPRELHAAMHYSV
                     FAGGKRIRPILCLASAEACGGDLAPVMPVACALELIHTYSLIHDDLPSMDNDDFRRGK
                     PTSHKVFGEATAILAGDALLTEAFALLSRAEKIRLAAERRLAVIQEIALAAGAAGMVG
                     GQALDIRTDQQAPDIGALTDMHRRKTGALIMAAVKTGALIAGANDRKLSALAGYANHI
                     GIAFQIADDILNVEGDETLMGKSTGSDAALGKVTCASLLGLEAAKARLAEEVGAATAC
                     IASFDARALPLRAIARFIMERKS"
     gene            complement(10514..10750)
                     /gene="xseB"
                     /locus_tag="PLA58_04790"
     CDS             complement(10514..10750)
                     /gene="xseB"
                     /locus_tag="PLA58_04790"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOR62143.1"
                     /translation="MAKEKFEEALDKLENIVKEMEAGDLPLDAALKSFEEGIRLIRFC
                     SAKLDETQRRVEQLLEKENSLQTKTFSDGQEDEA"
     gene            complement(10855..12186)
                     /gene="xseA"
                     /locus_tag="PLA58_04795"
     CDS             complement(10855..12186)
                     /gene="xseA"
                     /locus_tag="PLA58_04795"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOR62144.1"
                     /translation="MKDILTVSQLNENIKTFLEETFGILLVEGEVSNLRRPQSGHVYF
                     TLKDDKSQIRAVYFRPFGARAARFNLEDGMKILCRARLSAYVPRGEYQLIVESVEPQG
                     VGALQKAFEQIKARLAAEGLFDVSRKKPLPFLPRRIGVVTSPTGAVIRDILNITRRRF
                     PSVDILIAPARVQGAEAAGEIIAALSRLQTAGGIDVIIVARGGGSLEDLAPFNDEALA
                     RAVSCCTIPVVSAVGHETDFTICDFVADLRAPTPSAAAELVVPERAELRAGVDLLAER
                     LVAAQRRGLSTRRARLDTLQARFKDPRRFLADYAIHVDDLRERLKRALAHRTEVIAGR
                     LKPLNARLRGVDPRRRIREKGLLLAGLEKDLHGGWNHFFRDRRERLQNNAALLSSLNP
                     LAVLSRGYSITRRLPEGTIVREAHQLTTDSQISIQLARGGVEARVEKLRTE"
     gene            12368..13726
                     /locus_tag="PLA58_04800"
     CDS             12368..13726
                     /locus_tag="PLA58_04800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR62145.1"
                     /translation="MSENYDLIVIGGGPGGLTAARTAAALKKRVLIIEKTGWGGTCTH
                     RGCIPTKAMLSCSRFYADLKKLRRIGVNVSGATVDFAAMKKHRDQMVRIAALGAQKML
                     TGPQVESKTGLGEILSPREVRCIGEDGVQQFLKTEKILIAWGSAPQILPGLRTSERIR
                     TSDELLHLEQLPPSMIIVGGSFIGVEYATLFAELGVRVTLVELLDRILPREDEEAANL
                     LGQELARLGVVLHTSTRLVRLEESGDGVELSARKNGEDLAIRASYALLCTGRMPVLHK
                     DQLDVLGVDYTNAGIRVDENMMTSIGGVYAAGDVTGGMMLAHRASLQARVAVASMFGG
                     EIRQYDEDIIPSVVYSHPQIARVGLTERRALDEGLEISVIRSDYSANLMARAELMGRG
                     FVKMIFHREVIAGAVVAGDHAAELLAPLALAVSGKWTRRQFRSWVLPHPTLGEVFTPL
                     VD"
     gene            13746..14096
                     /locus_tag="PLA58_04805"
     CDS             13746..14096
                     /locus_tag="PLA58_04805"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOR62146.1"
                     /translation="MKIFHYTSAAPTHIDNDQAKGIDARVVIGRNDGADHFCMRIFEI
                     APGGYSPKHAHAWEHELFIHSGEGEAYDRGAWRPICPGTVLFIAPGEEHQIRNTGARK
                     LVFACLIPSGVPEL"
     gene            complement(14166..15035)
                     /locus_tag="PLA58_04810"
     CDS             complement(14166..15035)
                     /locus_tag="PLA58_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOR62147.1"
                     /translation="MKIQDVKNVCVVGAGNMGHQIALQCALSGFKTVSTDVVPAVLEK
                     AQKFVEGYLPGRVQKGKMTEEAAKKARSLISFTGDLKEAAKDADIVIEAVLERIDLKR
                     KVFADLDKYAPPAAILATNSSFIVSSRIADSTKRPSQVCNLHFFNPALVMKLVEVVQG
                     PHVSDETAQCMMDFCLKIDKIPIHIKKEVDGFVLNRIFAVIQREALWMLEMGVASFED
                     IDKACVYGAGHPMGPFRLNDLTGIDLTYDICMSHFLESGNPADLPSPSVVKKVTEGKF
                     GQKTGEGWYSYKK"
     gene            15235..15873
                     /locus_tag="PLA58_04815"
     CDS             15235..15873
                     /locus_tag="PLA58_04815"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR62148.1"
                     /translation="MPPSMTPKTVADKEETPRKSSALFLKRQSQIIKKATKLFMKQGY
                     AQTSMREISKATGIDIRNLYYFIRSKEEILFLVIEMIHRRELELFEKQDFTSIDDPVL
                     QLRTVIREFIGTGYHFGPEILLLYRESKSLPKRLLKVILSRESQVVACVEEILRRGRE
                     KKVFQFEDASFTANMIVYELSLHALRNWNMKQYAKEELIGLLENHVIKAVTV"
     gene            complement(15931..17043)
                     /locus_tag="PLA58_04820"
     CDS             complement(15931..17043)
                     /locus_tag="PLA58_04820"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOR62149.1"
                     /translation="MIQSRLCKMIGIKYPIIQAGMGPFSNNNLCVAAANAGVLGLLST
                     SGLFNKNDQPWIYKAFVESGEAGMDDDMATVMEKVLKRTYRLVRDKGGIFGINVMVSA
                     EVKPYSTIMIDTAIRVREENPDMKNNFKVIFTSAGDPVGWKDKIKGAGFTWMHVVPSV
                     KGALRCKKAGVDVIVASGHEGGFHTSWEPVHSMILLPAVVDAVSDENTLVVGAGGFCD
                     GRTLAAALVLGADGAQMGTRFLATKESDFHQIWKEGVVAAQDRGTLVARGFVGPARWL
                     KTPRSEEHAQNTLKKSPGVFLGTPDDYSSMDMSLIEFEIESIKAVYEGDREKALMAAG
                     EVAQRINDMPTVNDMVQGIVRDAEATLRSIEKKHLA"
     gene            17330..18109
                     /locus_tag="PLA58_04825"
     CDS             17330..18109
                     /locus_tag="PLA58_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR62150.1"
                     /translation="MLHGWPESSYCWEGVTEFLDPQWRVIAPDLRGLGDSERTLDVKA
                     YQKVELAKDVIEIINALGIQDFFLAGHDWGGIVAQEMAFLIPDRIKKFVILNIPVLTN
                     VKGAEEVTKAMIAMRYLPYWYQYFQMMPGLPEAMIRGKEREWVSFFFGKKGRDGTIPR
                     EAIDEYVRCYSIENTPATGASYYRSMSLDAPHWAGLAGKRFSMPSLYIYGNEDSVIIP
                     ANIAHLEDCFDSIQIEQIKAAHFVQEEKPGEVARLMNDFFK"
     gene            18198..18581
                     /locus_tag="PLA58_04830"
     CDS             18198..18581
                     /locus_tag="PLA58_04830"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HOR62151.1"
                     /translation="MSGIQSNKDQFRALADNPSQEPFVMLNLLKFKKEGGREAYFRYI
                     RESGPFVEAVGAKVVYFGKPKELLQGSEDWDLLMLVSYPSRKAFLSMANNPEYLKVHE
                     FRAEGVERAVLYATDPVKFKAILSE"
     gene            complement(19051..>20286)
                     /locus_tag="PLA58_04835"
     CDS             complement(19051..>20286)
                     /locus_tag="PLA58_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR62152.1"
                     /translation="KSWKALWCSVFVFALIVWGTAARAGEIGIGFTGPLSGPAAEYGQ
                     DCYNGVDMAVKEINAAGGVTVQGKKYTFKLEKLDDRADPTLAKNNAIRFVSQNKAIAV
                     FNPVATTIGALMAIPEQNFIIAAYSSVHTIMEKGHPMIINPVPNFVAYAKNFSIVAWD
                     RGWRTGAMVVTLGAYGDAWRNVFKAVWEKKGGKILADKPANYYTETDFSAQLTAAINT
                     KPDFLLIGGPSAPSALVIEQARSLGFKGGFILIDQAKPDYIAKTLGNKLTLLEGMIST
                     GDIKRLPLPIMPAYSKKYNAAYKGAVVNAENCLNYNMMHIIARAIAASQSLDAKVIRK
                     NIAKALPTAGDKFPNELFNIEENGVMNCGTIMQYVNNGQFSKVDYVISFPKTKEEFEK
                     IKKMSKSTDPKQIRYVPLQ"
BASE COUNT         4139 a         5619 c         5748 g         4780 t
ORIGIN      
        1 taacaactta tggaattcat cttccagtaa ccacctttag tgggtccaac gtgtgcgatc
       61 acgtttttac tttttatctc cttgtgtcgt gaccaaaagc gtccggcctt tatcggtaag
      121 ccggtatttt tgaagactgc ttgtcggttt ttccggcacc gtcagcgcga gaaggtcttc
      181 tgccagatga ggctttaaga cctggtccct gaacttggtc ctgtttgttc ttccggcaat
      241 cgccattaaa tcagttattg atggtgactc tatacactct aaaccgatta tttttttgca
      301 gacggtgttt ttaagcggac acaaacgttt tgatataatc gttctccccc gcttttggga
      361 ataacgctta cccattccgg atggtcatac tgatgccggt ccaggacttt tccattcttg
      421 tcgaaatcga tgatctcttc catccggatc cgccggtttg cgcaattcat ttcccataaa
      481 accgtttcat gatgatacgc tttatatttt atggatttct ccggatcgta ctttttgact
      541 tcttcaatcc tgtcttctct ggcttcatcc gttatggtcc tgtatgtcca aaccagcaga
      601 atgccgggtg acttcgttat tattttcttg ttgtaaaatg aattgtattg gagaggctcc
      661 catattttag gattgggcgg ttcggccgaa caaacggata aagaggaaag gatgaaaaaa
      721 aatataacgg aagtccacgt aatcttaagc cccatgactt gttgccctct aatgttttcg
      781 taacgtgttt caggcataac ctcgaatgga acatctatca atacaaaaaa gagcgctttg
      841 cggttcaccg cagctgatat aaagggctgt cctttccgaa gttgatgatc agcggcgtct
      901 gttcatggcc gatctgccgg gagatttgtg aagtcatccg ctgggaatat tcccccaaac
      961 ccacgatgct gaccttgccg tctttgtatt tgtccgcctg acggttgttg ttcaatccgt
     1021 ccagcagggt gtaagtgaaa agaccattgc cctgataccc gtccatcgcc gcctgctggt
     1081 cattggccga ggcgaaaata tgcagcccca tcttcctggc cagcacggac atccgggcgt
     1141 cgtaaagacc gctgatgatc gtgtccacgc cgcccgcgtg gcaggtatcg aagatgaaca
     1201 actggcttaa ggctttaatc ttctttgaca tttccacgat ttcgttggag ctgatcatgc
     1261 ctgcttcatc catccggccg ttgaaatcat gggtgagcat gtaatactga ttttgcagca
     1321 ggacgccgtg gccggccaca aaaaggataa agctgtcctg gggttggacg gctgtggaaa
     1381 gcgattggat tttgccggtg atgccggcct tgcttgcctg cgcgtccgtc aaaagcacaa
     1441 catgaatgtt ttgaggctga tacagcgtgg ccgactgtgc cctgattttc ccctccagat
     1501 ccgcggcatc cttgacggcg taccggagat tgacgctgct gtctttgtac tggttgatcc
     1561 cgacggagag aatgtagaga tgcggctctt ccgctttaat ccgggagctg aacgcggcgg
     1621 ttttcatata gctctggacg gtgttgccgc cgttgaaggc ggaaacgctg acttcattct
     1681 cgccggcaat ggcgtcgatc tcccggcagt cttcaaagac atcgcctttc ggttttccgg
     1741 aaatcggcga tacgtcggtt cgggccttga tgttgacgct gcgcatgttc gcgtaaatgg
     1801 atccgctgtc cagcgacaca agccgggccg gttcggcccc gattttggcg acttcccggt
     1861 aatagccgtc ggactgaacg agcttgccgt tgtggaacag gcgcacttcg ccgatgccgc
     1921 cgcccgcgct tgtggcctgg tagcagatcc tgaccttcgg cgaatccgtc tgctgcggca
     1981 aggccgtaaa ttcaaccgtc ggcggcgggc ttttcagcgc atcatcgatc gtgagcgtga
     2041 tcaggctcga gatgtcctcg cctttgagct ttgccgacac gatgtccggg cggtaaaaaa
     2101 catcatagaa ctggtcgatg ccatagacct tgctgcccac tttgatatgg agatattgat
     2161 ggccgttgag ggatgaattg taatagcctt ccggggtcag aacaatccac tcgccgtttt
     2221 gtaaactgac gaactgggcg atctccctgc cggcggagat gtcccagagt tttatcgcgc
     2281 cgtcctgtcc tccggtaaga aaataatttt catggcgcga gaagtccacc gcgggaacat
     2341 ttcccaaatg accggcaaac ttcttcagga ttctgccgga atccatatcc cacagtgaaa
     2401 gtgtgccgcc gaaagatttg gaggtgagca tgcggcgcgc atccggtgaa aaggctcccg
     2461 cccggatgcc ctgcgagccg cttttcgccc cggcggcatt gacatcatcc gcggtaaatg
     2521 tcctgatctc ccggcctgtg gcggcatccc agattttaac gactcggtcg ccgctgcgcg
     2581 agaaaacctg cctgccgtcc ggcgagaaag ccacagaggc gatttgccac tggtgtccgg
     2641 cgaaggtctg tatcctgtcg ccggtctgga catcccacag catcagtgtt ttatcaaagc
     2701 tccctgaaag caggaatctg ccgtccggtg aaaagacgat ggacttgacg ccgtccgtat
     2761 gcccggacaa cgttctgatt tcctttcctg aagcggcgtc caggattctg atggtgttgt
     2821 cccggctgcc ggaggcggca tattttccgt ccggagaaaa ggcgacgtcc agaatcagcg
     2881 cctcatgacc ggaaaacgtc cacagtgctt tatccgcggc gacatcccag catttcacgg
     2941 ttttatcaaa gctcccggaa aggatgattt tgccgtcggg agaaaaagcc acggcgttga
     3001 ccgcatctgt gtgtcccctg tacgtgcgga tttttttgcc cgcggcaatg tcccagagat
     3061 tgagggcgtc ttttttgtac cctccgccgg aaatgaggta tttgccgtcg ggggagcagc
     3121 ggatcgcgtt gattgtatcg ctgcccgtgg gggcgaaaat cttggcagcg atatcggcag
     3181 ccgtgatgga gggggactcc gctgtgacgg gtttcgcggc cgctgccgtt ttagtcgctc
     3241 ccgtgggatg tcccaccgtt cccgacagat attgcgcttt ctcgcccctg tgcgcgacca
     3301 gcaggacgat tttatctccg ggacttatgg cggcaacggc atcctgaaac tcgccgggcg
     3361 ttttgatgtc cttgtagttg acggaaagga taatatcatt gagccggatg cccatttttt
     3421 ccgccggact ttcattgacc acttcggaga caataacaac cggccaccgt ccatattcca
     3481 tgtgcatgat ccgcatatct tttcccgaaa ccggctgaac ggctacgcca aaccaggttt
     3541 tgtctgccgc ggatgccgcc gttgttgacg ctgcctgaac cggagaggcc ctgcctgcat
     3601 cggttccggg aattgccgcg acgggccggt caaccgggat ggccctgctt cccctggcgc
     3661 tcatccgatc ctgtgaaaaa atattctgcg gcgcaaggcc ggtgatcact gcgatcaaaa
     3721 gcagactgaa aatcattttc agaaaaagct tctttgtcat atgggtttct caatcaacgg
     3781 actgcacccc tgggataaaa ccaatggggt tggggcgtcg gctgggtttt tagtttatgc
     3841 accgttcttc aaatgccacg gacggtctgt atccaggaca gtgaatgcag acgttcgtgg
     3901 ttttacactt cctgaatgaa aaagaaaagc ctaatctgaa catcttccgg ggtgaggcag
     3961 gcagatgacc gaaaaacata ctttttttgc aagttaccca tgtttgcttc attattgaca
     4021 agtcttgctt tgtgttattt aatgcaacca ggcatgtgca aagcataccc cttctttttt
     4081 ataaaacttt tttagaaacc cggtaaagat ttcatccgga agaaaggaaa atcacatgcg
     4141 gccgattcgt gacattctgg aagcgcaaag cgtcgtcgtg atcggtgcgt cgcgggatca
     4201 ggaaaaaccc ggcgcacagc ttttaaaggt gttgaaggac gtgggctttg cgggccgtgt
     4261 ggccggcgtc aatccgcagg ggggaaatgt gttcggtgtc ccgctgtatc catgcgtgcg
     4321 ggacgtgccg tttgaaatcg atctggcggt tttgctcatt ccaccccgtt ttgtccctga
     4381 cgccttgcgc gattgtgccg tcaagggcgt caaaggggcg gtgatctccg ccgaaggttt
     4441 cgcggaaacg ggaacgcagg gggcaaccta tcaggaggat gtccgcgcca ttctgaagtc
     4501 ctccggcatg agaggtttcg gccccaacac cctgggtctg gtcaataccc aaaccaaact
     4561 gaccacgtcg tatttcgcca attcccagat gcttgcgtcg ggtcatatcg gttttgccgc
     4621 gcagtccggt atttttgtgg gcgcgctcct gcgctacctg agttcgctta caggccttca
     4681 tctgtccaaa ggcatcggcc tgggaaacaa agtggatgtc aacgaatcgg acgccctctc
     4741 ctttctggct gacgatgagc agacgaaaat catcgggctg tatctggaag acgtaaaaga
     4801 cgggggccgg tttgtgaaaa atctgaaaaa ggcgacggcc aggaagccgg tgctgattat
     4861 caaaagcggc cgcacgcagg ccggcgcgaa agcgtccgca acacacaccg ccagcatggc
     4921 ggtggaagat tccgtgtttg atggaatcgt tcggcaggcc ggcgcgattc gccttcatgc
     4981 cattgacgag tttgtccgga cgctgaaagg ttttttgaat atgccgcttc ccgcgggcga
     5041 ccgcctggct tttgtttctt acagcggcgc gcaggcgatc atgagcattg acacgacgat
     5101 tgaacaggga ttgcaagccg ccagactggc ggatgccaca caagcccgga tcggccgggt
     5161 gatcgcaacg tcctccaaga caaaaaatcc catcgacatc tttcccgact ggctgacgca
     5221 cggctacgag aaaaccatca cggaagtggt gaaagccctc ctggatgacc ggggcgttga
     5281 cggaattgtg ttcatatcct ttgccgaagc gaatcccgag gtttatcagt ccctggtcaa
     5341 tgttgttcaa gcggaccgcc gcaagcccgt gtttgtgtcg cttctgggag acagcctgga
     5401 caaagcggcc tgcgaacgtc ttttagacgc aggaggcctt ccctgcttcg attttccgga
     5461 gcaggccgtc cgtgtcttcg cccgcatgcg gcaatacgcc aagagaataa aggggacaga
     5521 ataaagagga ggttcagttc tattttaatc tctccgggtc aattccccga agcttgctgc
     5581 ggggcctttc atttgaataa aaaattagaa ataaatagaa ccgtccctta aattttaaaa
     5641 aaggggcgcg gggagaattt cccagcgccc cttttccggt ttggataggg tttgctgatc
     5701 gatcagttgc tgtaaagcgc gatcagctga tcgccgtatt ttttcatgat gttgcgccgg
     5761 atcagtttca tcgtctgggt catcatgccg ttatcgactg tgaaatcctc ggtgatgtaa
     5821 aggaatctct gaggaatttc atatccgcca aacgttttgc gcagatgctc ggtgatttct
     5881 ttggagatga aatccgtgca ttccttgttg gcgatggttt cttcccggga gccctgtgcc
     5941 caggcggacg ttttgggatc ggccttcaac gcttcaaagt ccggaacgac cagcgcgaca
     6001 ttgaacaagc gcccctcccc gtaaacgatg gcattggaga tatacctcag aagcttcatt
     6061 tcgttttcga tggattcggg atgcacgtac ttgccgtttt ccagcttgta ttcgtccttg
     6121 aaccgaccgg taatgtgcag atatccgtct tcgtccagcc agccgcggtc tccggttctt
     6181 acgccgggaa aaccgttcca ggtatccggc atcatggctt ccttggtgac ggccggttta
     6241 ttgagataac cctgcatgac ctgcgcgccg tacaccacga tttctccgtc cggactgtct
     6301 tctcccacgc gggatttgtc gatgaccaca tgggtgtcct tgaaaggctt gccgaccgtt
     6361 ccgtatttgt tgccccgctt gggcgagttg ttggagatga cgggcgtggt ttccgacaga
     6421 ccgtagccgt cgtaggtggg cacgccgaca tcggcgaaga acagggcgat ttccggcttc
     6481 atcatggcgc cgccggtaac cacatgcttc aaccgtccgc cgaagatgtt ccgaatcgtg
     6541 gaaaaggcca ccgcgtcgta gaatttgaat tcttccgttt tttccttcaa gtcccggttt
     6601 ttgaccgcct cggctaccgc cgcgtcaaag actttctttt tttccggatc cgcggctact
     6661 ccctgctgga ttttatcgta gataatgttg aagacgcgcg gcacggccga catatgcgta
     6721 ggcttgacct cgctgaagtt gatgatcagt ttgtcggcgg attcggcaaa tgcaagggcc
     6781 cagccgccca ggatgtacgt gtgcagatcg gcggtcaaac cgaaactgtg cgcccaggga
     6841 agaatgcaca aacccacttc atccggatct ccctcgtcga aagtctcgtg gcactcgcgg
     6901 gcgttgtggg tcatgttgcc gtggtgcagc atcacgcctt tcggatcgcc ggtggtgccc
     6961 gaggtgtaga tgataaaggc cgtctccgac cagtgcggct tgtaggaagg ggcggggttt
     7021 tccttgcccg tcttttccag cgccgccagt gatttttcac cctcaccaaa aataataaag
     7081 acatccttta aagtcggcat atctttcttg aatcccatga cgacgtcata gatggtctgg
     7141 ttgctgacaa aaaggtactt aatgccggcg tccgaaataa tgtactgcca gaccttttgc
     7201 agctccttct cgtacatggg cacaaaaacg ccgcccagac cgtgggtggc attggcgcac
     7261 acaaaccact ccgtacaatt gctgacgatg acaccgactt tttcgccctt gctcaatccc
     7321 agcttgttga gagcgccccg caggttgttc acccgttcgg cgacggcgct gtacgtcacc
     7381 cattcgtatt gattggtggc cttgttcttg gtgccgaaca gattgtggct ggcgtagcgg
     7441 gccgcgcttt cctcaaagag atcgaccaga ttattgggtt tagtgtagta cgcgtccagg
     7501 ttcgcggacg caccttttcc tgctgtcgct tctaccattg ctgtgcctcc ctgtttttct
     7561 tgatccatta atattgttcg gccagacctg tcaggcctgg ccgttcatgg tctttatatt
     7621 ttatagtcac aacaacgcgc atggtgtcaa tgaaaaataa tgtttttagt tctttcagca
     7681 gtgaagaatt tctctgaaga ttcacccgtt cacttttgga gcatttctct ggccgcccgg
     7741 aggatgccct cttcatccag gccgagttga gcgcgcaatt cgctctgctt ggcgtgctcg
     7801 acaaattcat cccggacgcc cagcctccgg acggttatgc cggtcacatc gttttgggca
     7861 aacagctcca ggaccgcgct gccgaatccc ccgtccagca cgttttcttc gatggtcaga
     7921 attttcttta cggaagcggc cgtgccgagc aacagttccg cgtcgagcgg tttgatgaag
     7981 cgcgcgttga tgactttgac cccgatccct tccgccgcca gcttccgggc ggcctgcagg
     8041 gccggataga cggtcgagcc tacggccatc atggccagat ccgttccttc gtggagcact
     8101 tcgcccctgc ctaagggcag cgttgtaatc tcgctgtcga ggggaacgcc cacgcctttg
     8161 ccccgcgggt aacggatgga aacgggagaa ccgcagccga cggccgtttt gagcatgtgc
     8221 cgcagttcgt tttcatcttt cggcgccatc atgaccagac gggggaggtt gcgcagatag
     8281 gacagatcaa aaagcccctg atgggtcgct ccgtcttccc cgacaatgcc cgcgcggtcc
     8341 acggcaaaaa caaccggaag attttgcaga cagacatcgt gcagaatctg atcgtaggct
     8401 cgctgcagga aagtagaata aatcgccacg acgggcgtca gtccttcggt ggccaggccc
     8461 gcggcaaagg tcacggccaa ctgttcggcg atgccgacat cgtaaaaccg gccgggatgg
     8521 gccttgcgga atttttccag ccccgtgccc tcgcacatgg ccgccgtgac ggcgacaatc
     8581 cgggaatcgg ccgcggccag ttcggtcatt gtccggccga aaatttccgt gtaggaaggc
     8641 gcggggttga ccgaaggaag ggtctggccg gtttccacgt caaagggcgc gatgccgtgg
     8701 taggtgggag aattttcttc cgcgaatttg tagccccggc ccttttgcgt gatgacgtgg
     8761 accagcacgg ggccttccag ctttttgacg ttttcaaagt ttttgagaag gtaatccaga
     8821 cggtgcccct ccagggggcc gacataggta aaccccagct cctcgaacag ggcgccggga
     8881 accacaaagg ttttcaccga ttcttcgatc tgctgcgaga acttcaaaat ctgctcaccg
     8941 atgccgggaa tgcttttgaa gaaatttttc agttcacttt tgaatcgggt gacccggtcg
     9001 ccggtcatga ttctgttcaa ataggacgag agagcgccga cgtttggcga gatggacatt
     9061 tcattgtcgt tcagcacaat gatcaggttt tttttccggt cgcccgacca gttcaatccc
     9121 tcgaacgcca tgccggtggt gagggagccg tcgccgatca cggcgatgat tttgttgtgt
     9181 tcgcccttca ggcactgggc ttcggcaaag gccgccgcgg ccgcgatgga ggtgccgctg
     9241 tgcccggcat tgaacgtgtc gtaggggctc tccgtccgct tcgggaagcc gctgaggccg
     9301 ccctgctggc gcagcgtggc aaacttttcc ttgcggccgg tgacgatttt gtgcgcgtag
     9361 gactgatgac cgacatccca gatcagccgg tcccgcggcg tgttgaagac gtagtgcagc
     9421 gcgagcgtca gctccacggc gcccagtgac gaagccaggt gcccgccggt ggccgcaacc
     9481 gtggagataa tcaggtcgcg gatttcccct gccagtttct gaagttccgc gacggaaagg
     9541 ctgcggaggt cggccgggtc gttgacgctg tccagcacac tgtaagggat gatttccgga
     9601 ggtttcattg gtttttacaa gcctttacga ttttcgttcc atgatgaagc gggcgatggc
     9661 gcgcagcggc agggcgcgcg cgtcaaagga agcgatgcag gctgtggccg ccccgacctc
     9721 ttcggccagc cgggctttgg ccgcttcgag tcccagcagc gacgcgcagg tgaccttccc
     9781 cagagcggcg tcgcttccgg tgcttttccc catgagggtc tcgtcgcctt ccacattgag
     9841 aatgtcgtcg gcaatctgaa aagcgatgcc gatgtggttt gcgtaaccgg ccagcgccga
     9901 aagcttccgg tcgttcgcgc ccgcaatcag cgcgccggtt ttgaccgccg ccatgatgag
     9961 cgcgccggtt ttacgccggt gcatgtccgt cagggccccg atgtccgggg cctgttggtc
    10021 cgtccggata tccagcgcct gcccgcccac catgcccgcg gcgcccgcgg ccagagcgat
    10081 ctcctgaatg acggccaggc gccgctccgc cgccaggcgg attttttccg ccctggacaa
    10141 gagcgcgaag gcttccgtca ggagcgcgtc gcccgcgagg atcgccgtcg cctcgccgaa
    10201 cactttgtgg ctggtgggtt tgccgcggcg gaagtcgtcg ttgtccatcg acggcaggtc
    10261 gtcgtggatc agggaatacg tgtggatcag ctccagcgcg caggcgaccg gcatcaccgg
    10321 cgccaggtcc ccgccgcagg cttccgccga ggccaggcaa agaatcggac ggatcctttt
    10381 gcccccggcg aaaacgctgt agtgcatggc ggcgtgcagt tcccggggct cattgtcctt
    10441 gcctggaaga taacgttcga gcgcttcttc cacaatcttg caccggtcct gtaaataggc
    10501 ctcaagaaaa tcctcatgct tcatcttctt gcccgtccga aaacgttttg gtttgcaggg
    10561 agttttcttt ttccagcaac tgctccacgc ggcgctgggt ttcatcaagc tttgccgagc
    10621 agaagcggat caggcggatg ccttcttcaa aggacttcag cgccgcatcc agcggcaggt
    10681 cgcccgcttc catttctttt acgatatttt ccagcttgtc gagcgcttct tcaaattttt
    10741 ctttcgccat tccttttcac tcccctcata ttttttatgg cggtcccctc cgctgcgttt
    10801 ccctctccca tcaaaagagg ggaggatgcg atgagctgct agccatgagt cattttactc
    10861 cgtccgtagt ttttcgaccc gcgcctcgac gccgcccctg gccagttgaa tactgatttg
    10921 cgaatccgtt gtcaactggt gtgcctcccg gacgatggtt ccctccggca gacggcgggt
    10981 gatgctgtat cccctggaca ggacagccag cggattcagc gatgacagaa gcgcggcgtt
    11041 gttttgcagc cgttcgcggc gatcccggaa aaaatggttc cagccgccgt gaaggtcctt
    11101 ttccaggccg gcaaggagca ggcctttttc gcggatccga cgccggggat cgacgccgcg
    11161 caggcgtgcg ttgagcggct tcagacggcc ggcgatgacc tccgttcgat gcgccagggc
    11221 gcgcttcagc cgctcgcgca gatcgtccac gtgaatggcg taatcggcca gaaagcggcg
    11281 cgggtctttg aaacgggcct gcagggtgtc gagtcttgcc cgtcgggtag aaagcccgcg
    11341 gcgctgggcc gcgaccagcc tttccgcaag aagatcgacg ccggcgcgca attcggcccg
    11401 ttcgggaacg accagctccg ccgccgcgga aggcgtgggc gcgcgcaggt ccgcgacaaa
    11461 atcgcagatg gtaaagtccg tttcatggcc gaccgccgaa acaacgggaa tcgtgcagca
    11521 agaaacagcg cgcgccagcg cttcatcgtt gaacggcgcc aggtcctcca gcgaaccgcc
    11581 gccccgcgcg acaatgatca catcgattcc gccggccgtc tggagtcgcg acagcgccgc
    11641 gatgatttcg cccgccgcct cggcgccctg cacgcgggcc ggcgcaatca gaatgtccac
    11701 cgaagggaag cgccgtcgcg taatgtttaa aatatcgcgg atgaccgcgc ccgtgggcga
    11761 cgtgaccacg ccgatgcgcc ggggaagaaa gggcagaggc tttttgcggc tgacatcaaa
    11821 caggccttcg gcggcgagtc tggctttgat ctgctcgaag gctttttgca gggcgcccac
    11881 gccctgcggt tccacggatt ccacgatgag ctggtattcg ccgcgcggaa cataggcgga
    11941 gagccgcgcc cggcagagga tcttcatgcc gtcttccagg ttgaagcggg ccgcgcgggc
    12001 gccgaagggg cgaaaataca ccgcgcggat ctgacttttg tcgtccttga gcgtgaaata
    12061 gacatgtccc gactgggggc ggcgcagatt ggaaacctcc ccttccacaa gaaggattcc
    12121 gaaagtttcc tccaggaagg ttttaatgtt ttcgttcagc tgtgatacgg tcagaatgtc
    12181 tttcatcgat tcagtcctgt cgatcgaact accagatatc ggcggttgtg acaagctgca
    12241 attccgcctc aaaatgcctg cggcttttgg cccgcagcgg cctgttttgt tttggcggcg
    12301 cgggcggaca aattccgttt gcccggtccc caagcctgtg ttaaacattt ccccggggag
    12361 gaaaatcatg tccgaaaact acgatctgat tgtcatcggc ggagggccgg ggggactcac
    12421 ggcggcccgg acggcggccg ccctgaaaaa gcgggtgctc atcatcgaaa aaaccggctg
    12481 gggcgggacc tgcacgcacc gcggctgcat tcccaccaag gcgatgcttt cctgcagcag
    12541 gttttatgcg gatttgaaaa agctcaggcg aatcggcgtg aacgtctccg gcgcgaccgt
    12601 cgattttgcc gccatgaaaa aacaccgcga ccagatggtt cgaatcgcgg ctctgggagc
    12661 gcaaaaaatg ctcaccggcc cgcaggtgga gagcaaaacc ggactcggag aaattctttc
    12721 accccgggag gtgcgctgca tcggtgaaga cggcgtccag cagtttttga aaacggaaaa
    12781 gattctgatc gcctggggat cggcgccgca gattttgccg ggcctccgga cgtcggagcg
    12841 catccggacg tccgatgaac ttctccatct cgaacagttg ccgcccagca tgatcattgt
    12901 cggcggcagt tttatcggcg tcgagtacgc cacgctgttt gccgaactgg gcgtccgggt
    12961 gacgctggtg gagctgctgg accgcatttt gccccgggag gatgaagaag cggcgaatct
    13021 gctgggccag gagctggcgc gcctgggggt cgttctccat acctcgaccc ggctcgttcg
    13081 cctggaagaa tcgggggacg gcgtcgaact gtcggcgcgg aaaaacggcg aagacctggc
    13141 gatacgggct tcttacgctc tgctttgcac gggccggatg ccggttttgc acaaggatca
    13201 gctggacgtg ctgggggtgg actacacgaa cgcgggaatc cgtgtggacg aaaacatgat
    13261 gaccagcatt gggggcgttt acgcggcggg cgatgtcacc ggcggcatga tgcttgcgca
    13321 ccgggcgtcc ctgcaggcca gggtcgccgt cgcaagcatg ttcggcggag aaatccgcca
    13381 atacgatgaa gatatcattc catccgtggt gtacagccat ccgcagattg cgcgggtggg
    13441 gctgaccgag cggcgcgccc tggacgaagg tctggaaatc agcgtgattc gctccgacta
    13501 cagcgccaat ctgatggccc gcgccgagct gatgggccgg gggtttgtca aaatgatttt
    13561 ccatcgggag gttatcgcgg gtgcggtggt ggccggcgac cacgcggccg agcttctggc
    13621 cccgcttgcg cttgccgtat ccgggaaatg gacgagaagg caatttcggt cgtgggtgct
    13681 tccgcacccg acgctcggcg aagtctttac gcccctggtg gactaaacgg gaaaaggagg
    13741 actgtatgaa aatttttcat tacacatccg cggcgccgac gcatattgac aacgatcagg
    13801 ccaaaggaat cgacgcccgc gtcgtcatcg gcaggaatga cggagcggac catttttgca
    13861 tgcggatctt tgagatcgcg ccgggaggat attcaccgaa gcatgcgcat gcctgggagc
    13921 atgaactctt tattcattcc ggcgaggggg aagcttacga cagaggggcc tggcgcccga
    13981 tttgcccggg aacggtgttg tttatcgcgc ccggagagga gcaccagatc cgcaacacgg
    14041 gagcccggaa gctggttttt gcctgcctca tcccgtcggg tgttccggaa ttgtgaggcc
    14101 gcggtcctgc tccccgcagc ggcggggaac aggtgcggca ttggaatttg cttgccaatc
    14161 ttgaattatt tcttgtagct gtaccagcct tcgccggtct tctgtccgaa tttgccttcg
    14221 gtgactttct tcacgacgct gggggaaggc aagtcggccg gattgccgga ctccaggaaa
    14281 tgactcatgc agatgtcgta ggtcagatcg atgccggtca gatcattgag gcggaacggg
    14341 cccatcgggt gccccgcgcc gtaaacacag gccttgtcga tgtcttcgaa ggaggccaca
    14401 cccatctcca gcatccacag cgcttcccgc tgaatgacgg cgaaaatccg gttcaggaca
    14461 aagccgtcca cttctttctt gatatgaatg gggattttat cgatcttcag gcagaaatcc
    14521 atcatgcatt gcgccgtttc gtcggaaaca tgcggtccct gaacgacctc gaccagcttc
    14581 atgaccagcg ccggattgaa aaagtgaaga ttgcagacct gggaggggcg tttggtggag
    14641 tcggcgatcc gggagctcac gatgaaggag ctgttggtcg ccaggatcgc agcgggcgga
    14701 gcgtacttgt ccagatcggc gaaaactttg cgtttcaggt cgattcgttc cagaacggct
    14761 tcaatgacga tgtccgcgtc tttcgcggct tctttcagat cgccggtgaa cgaaatcaga
    14821 ctccgggcct tctttgccgc ctcttccgtc atttttccct tctgcacgcg gcccggcagg
    14881 tagccttcga caaatttctg ggccttttcg aggacggccg gcacgacatc cgtgctgacg
    14941 gtcttaaaac cggaaagggc gcactggagt gcaatctgat gccccatgtt gcccgcgccg
    15001 acgacgcata cattttttac atcctggatt ttcatttttt cctccttatg ttgttaacgg
    15061 attggttgat gttcttcact acgaagaaaa cggtggccgc gaggcgttcc tgtacagcga
    15121 tcgttcgttg tatcaattat tttgaagggg tcattttgtc aagtaatatt ttcaatttac
    15181 attgacagaa agaagcgttc tgttgtaagg aactttcaat tcagtgaaag gaccatgccg
    15241 ccatccatga cccccaaaac cgttgcagac aaggaggaaa ctccgcggaa aagcagcgct
    15301 ctgtttctca agaggcaatc ccagatcatc aaaaaagcca cgaagctttt catgaaacaa
    15361 ggctatgcgc agacgtccat gcgcgaaatt tccaaggcca cgggcattga catccgcaat
    15421 ctgtattatt ttatccggag caaggaggag attctctttc tggtcatcga aatgattcac
    15481 cgccgcgagc tcgagctttt cgaaaagcag gatttcacga gcatcgacga ccccgtgttg
    15541 cagttgcgaa ccgtcattcg cgagtttatc ggcaccggct atcatttcgg gccggaaatc
    15601 ctgcttctgt acagggaatc gaaatcgctt cccaagcgcc tgctcaaagt gattctttcc
    15661 cgggagagcc aggtggtcgc gtgcgtcgag gagattctgc ggcgcggccg ggagaaaaag
    15721 gtgtttcaat ttgaggatgc ttcctttacc gccaatatga ttgtttatga attgtccctc
    15781 cacgcgctgc gcaactggaa catgaagcaa tatgccaagg aggaactgat tggtttgctg
    15841 gaaaaccatg tgattaaagc ggttacggta tgacgccgtt gccgcgaagg gggaaaagcc
    15901 gggagtcgcc tcccggcttt ttttattgcg ttacgccaga tgctttttct ctatgctgcg
    15961 cagcgttgcc tctgcgtccc ggacaatgcc ctgcaccatg tcgttcaccg tgggcatgtc
    16021 gttgatccgc tgggccactt cgcccgcggc catgagcgcc ttttcccggt cgccttcgta
    16081 aaccgccttg atggattcaa tctcgaactc gatcagagac atgtccatgc tggaatagtc
    16141 gtcgggcgta cccagaaaaa cgccggggga tttcttgagc gtgttctggg catgctcttc
    16201 gctgcggggt gtcttcagcc agcgggccgg tcccacaaag ccacgggcaa ccagcgttcc
    16261 ccggtcctgg gcggcgacga cgccctcttt ccagatctga tgaaaatcac tttccttggt
    16321 cgccaggaat cgcgtgccca tctgcgcgcc gtcagccccc agaaccagcg cggcggccag
    16381 cgtcctgccg tcgcagaacc cgccggcgcc cacgaccagc gtgttttcgt cggacacggc
    16441 atccaccact gcgggaagca ggatcatgga atggaccggc tcccaggacg tgtgaaatcc
    16501 gccttcatgg cccgaagcga cgatgacgtc cacccccgcc tttttgcagc gcagcgcgcc
    16561 cttcacggaa ggaaccacgt gcatccaggt gaagcccgcg cccttgatct tgtctttcca
    16621 gcccaccgga tcaccggcgg aggtgaagat gaccttgaag ttgttcttca tgtccggatt
    16681 ttcctcccgg acccggatgg ccgtatcgat catgatggtc gaatagggtt tgacttccgc
    16741 cgaaaccatg acattgatcc cgaaaatgcc gcccttgtcc ctgaccaggc gataggtgcg
    16801 cttcaaaacc ttttccatca cggtggccat atcgtcgtcc atgcccgcct ctccgctttc
    16861 cacgaaggcc ttgtaaatcc agggctgatc attcttgttg aaaaggccgc tggtggaaag
    16921 aaggcccaga acaccggcat tggcggcggc gacacaaagg ttgttgttgg aaaacgggcc
    16981 catgcccgcc tggatgatgg ggtatttgat cccgatcatt ttgcatagtc tcgattgaat
    17041 cataaaacat ctcctcttgt ttttattggg ttgattccgg tctttctttt tacaacgagc
    17101 gttgtgatga ttaataggtc ggggaagttt tgtcaagtaa tatttcggtt ttaggggaag
    17161 gcttgttaaa attccttgac aaaacccttc cggataaata atacaacacc cgttgtataa
    17221 atcatgaagc acggggacgg tgaagactat gaaaacaggc gcgattcgtg ttccgcgggg
    17281 aacctttcat gtccgggatc ggggaaatgc ggacgggttt ccagtggtta tgctgcacgg
    17341 ctggccggaa agctcttatt gctgggaggg tgtgacggaa tttctggatc cgcaatggcg
    17401 cgtgattgct ccggatttgc gcggtcttgg cgacagtgag cgcacgctgg atgtcaaagc
    17461 ttatcagaag gtggagctgg ccaaagatgt gatcgaaatc atcaacgcgc tgggcattca
    17521 ggatttcttt ctggccggcc atgactgggg cggcatcgta gcgcaggaaa tggcctttct
    17581 gattcccgac cggatcaaaa aattcgtcat tctgaacatt cccgtcctca ccaacgtcaa
    17641 aggcgcggaa gaagtcacca aagccatgat tgccatgcga tatcttccct actggtatca
    17701 atactttcaa atgatgccgg gactgccgga ggccatgatt cggggcaaag aacgcgaatg
    17761 ggtcagcttt ttcttcggga agaaaggcag ggacggcacc atcccccggg aggccatcga
    17821 cgaatatgtc cgctgctaca gcatcgaaaa tacgccggcc acaggggctt cctattaccg
    17881 ctccatgtcg ctggacgccc cccactgggc gggcctggcc ggcaagagat tttccatgcc
    17941 ttcgctctat atttacggca atgaagactc cgtcattatt ccggcaaaca ttgcccacct
    18001 ggaagactgc tttgattcga tccagatcga gcaaatcaag gcggcccatt tcgttcagga
    18061 ggaaaagccc ggagaggtcg cccgcctgat gaacgacttt tttaaatgat tcatgaaacc
    18121 cggagcgcag acggcgtcca accccatcca tgaccggcgt cgcctctttc cacaaatcgg
    18181 tttaaggaga aaacatcatg tccggtatcc aatcgaacaa agatcagttt cgggccctgg
    18241 ccgacaatcc cagccaggag ccgttcgtca tgctcaattt gctgaagttt aaaaaggaag
    18301 gcggccggga ggcctacttt cgctacatcc gggaatcggg accctttgtc gaggcggtcg
    18361 gcgccaaagt ggtttacttc ggaaagccga aagaactgct ccagggaagc gaggactggg
    18421 atctgctcat gctcgtttcc tatccgtcgc gcaaggcgtt cctttccatg gccaataatc
    18481 ccgagtatct caaggtgcat gaattccggg ccgaaggcgt ggagcgggcc gtgctctatg
    18541 cgacggaccc tgtgaaattc aaggccatcc tgtccgaata gcgcagagaa ccgcgcaacc
    18601 tctgggaaaa taagcgcctc tgtctgtgac cccggcacca ccggtgatcc caaccgcggc
    18661 gatcgcggtt tcccatttcg ccccctgaaa gtcgtggatc actgcggaga acagctccaa
    18721 gcgctctacc ggagctgacg cgcaaacaaa acaaaggtca acgggctgtt gcccaaacac
    18781 ccttttcgct tgcctgtgag cgttttgtat aaatctaagg ctcgcgaaag gctttgacaa
    18841 aactcgcctt cggctcaaac agttgtcatt gctgatcttt cgcttcgcca agatttatat
    18901 aacaaaacgc tcccaatggc cgctcaaaga gatagctttg ggcaacagcc cgtcaaaaca
    18961 aaacccccgt tctcgctgtg gagaacgggg gttctttttt acccgttgaa ttctgcgcgg
    19021 attacgtcgg tttgattccc ggcggataag ctactgaagc ggcacgtagc gaatctgctt
    19081 cgggtccgtg ctcttgctca tctttttgat tttttcaaat tcctctttgg ttttcgggaa
    19141 ggaaatgacg tagtccacct tgctgaactg gccgttgttg acgtactgca tgatggtgcc
    19201 gcagttcatc acgccgtttt cttcgatatt gaacagttcg ttggggaatt tgtcgccggc
    19261 cgtcggcagg gctttggcaa tattcttgcg gatcaccttg gcgtccagcg actgcgacgc
    19321 ggcaatggcg cgcgcgatga tgtgcatcat gttgtagttg aggcagtttt cagcgttgac
    19381 cacggcgccc ttgtaggccg cattgtattt cttgctgtag gccggcatga tgggaagggg
    19441 cagacgtttg atgtcgcccg tgctgatcat gccttccaga agcgtcagct tgttgccgag
    19501 tgttttggcg atgtaatccg gtttggcctg gtcaatcaga atgaaaccgc ctttgaagcc
    19561 caggcttctg gcctgttcga tcaccagggc gctgggcgcc gaaggaccgc ctatcagcag
    19621 gaaatccggc ttggtgttga tggccgccgt caactgcgcc gaaaaatccg tttccgtgta
    19681 gtagttggcc ggtttgtcgg ccaggatttt gccgcctttt ttctcccaga cggccttgaa
    19741 tacattgcgc caggcatctc cgtaagcgcc cagcgttaca accatcgcgc cggtgcgcca
    19801 gcctctgtcc caggccacga tggagaagtt tttggcgtag gcgacaaagt tgggaacggg
    19861 attgatgatc atcggatgac ctttctccat gatggtatgg acgctggagt aggcagcgat
    19921 aatgaagttc tgttcgggaa tggccatcag ggcgccgatc gttgtggcaa cggggttgaa
    19981 aacggcaatg gccttgttct gactcacaaa ccgaatcgca ttgtttttcg ccagggttgg
    20041 gtcggcccgg tcatccagct tttccagctt gaaggtatat tttttgccct gaacggttac
    20101 gccgcccgcg gcattgattt cttttaccgc catatccacg ccgttgtagc aatcctgccc
    20161 gtattcggcg gccggcccgc tcaaaggacc tgtaaaaccg attccgattt ccccggctct
    20221 cgcagccgtt ccccacacga tcaacgcaaa gacgaatacc gaacaccata acgctttcca
    20281 actctt
//
LOCUS       DAOSRM010000033        20207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2834_length_20207_cov_112.286721, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000033 DAOSRM010000000
VERSION     DAOSRM010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 20207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20207
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2834_length_20207_cov_112.286721"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1252
                     /locus_tag="PLA58_04840"
     CDS             194..1252
                     /locus_tag="PLA58_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOR62153.1"
                     /translation="MELLGLMEKVYLGNTLQNWIIGVCVLVGVFCALKILQRIAIARL
                     SKLAAATDNQIDDLLVSMLGQTKFLILLVASAYVASQVITIKPSVEAVWQKIVGLIVI
                     IQFGLWASAGVTFALGRAIEKRMDRDAGSATTLTLLGFVARLILWVIVLLLILDNLGV
                     NITGLVAGLGIGGIAVALAVQNILGDLLASLSIVLDKPFVIGDFIVVDSLSGTVEHIG
                     LKTTRIRSLSGEQLVFANNDLLKSRIRNYKRMQERRIVFGFGVVYSTPLEKLTLIKKM
                     VGDIVSGTENARLDRIHFKEYGSSSLNFEVVYFVANPDFGLYMDIQEAINLEIFRRFA
                     EEGIEFAYPTQTIYVHHP"
     gene            1301..2695
                     /locus_tag="PLA58_04845"
     CDS             1301..2695
                     /locus_tag="PLA58_04845"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOR62154.1"
                     /translation="MCAASIFSPDLCLAAGAAPNIGAVLPLWSMLPFAGILLSIALVP
                     LVLPRFWHRHFPKVSAFWAIVFAVPFIYFFRETALYEIGHIMIVDYIPFILLLWSLFT
                     VAGGIYIKGTLKGTPAVNVMILIIGTLLASVIGTTGASMLLIRPILRSNAWRVYKVHT
                     IVFFIFLVSNIGGSLTPLGDPPLFLGFLHGVPFFWTMNILPEMAFASIILLILYFLLD
                     SYYFRKESKTAVPEDESEPLGIEGKRNFIFLIGVIGAVLFSGSVKLGEISVFGIHQTV
                     ENLIKDAVLIAMGILSLWLTGRDVRKGNEFTWAPILEVAYLFAGIFITIIPALAILKA
                     GEQGALAWMIRSVNSPAEYFWAVGLLSSFLDNAPTYLTFFNSALGKFYPGIPEAQAVA
                     GLIVEQIPYLAAISAGAVFMGANTYIGNAPNFMVKSIAEEAGVKMPSFFGYMFKYSIP
                     ILVVLFIVITFIFF"
     gene            2842..3699
                     /locus_tag="PLA58_04850"
     CDS             2842..3699
                     /locus_tag="PLA58_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HOR62155.1"
                     /translation="MGSTLRKSLKTIEEYKTANPHYTDLLDILGDILILREEYRKKMT
                     KPIFSVEDKLIPGKMAGGLPLIDLAGNKYDLTRPKEYFDSLISIAQKRMPAEAHRFLD
                     VIKNEQFDWEAIIRESFNPAPQSEDVPEREARASGEHDEQIDLIDLFIEESLRPELEV
                     VAEKYGALVEKSDWTEGYCPICGKEPKIGEIRESEDGRRYLFCNQCGYKWHFRRIKCP
                     FCGNEEQHSLAYFAVEGEESHRVDVCNKCRRYIKIIELPKSSEGVNLDVEDIATLHLD
                     MLAYEEGYN"
     gene            complement(4047..4961)
                     /locus_tag="PLA58_04855"
     CDS             complement(4047..4961)
                     /locus_tag="PLA58_04855"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOR62156.1"
                     /translation="MKKAKIVEVAPRDGLQNLQAFVPTDQKIALIKKLAGTGAQEIQI
                     GSFVSPQAIAQFRDMEEVAQAVRRLKGFVPSALVPNGRGADKALACGIARLIFFFSVS
                     SKHNYANVQQSPLDSLKELQHICRSHLSNPSMTLRVDLATAFGCPFSGNVTTAAVLRT
                     VEKVAALGVREITLCDTVGFGHPKDVEEKFLACFRRFPEIRFGAHFHNTRGLAQANAL
                     RAYDIGVRSFDSSAGGLGGCPYAPGSSGNVATEDLVFMFESMGIKTGMDLKKLLNVCA
                     WLQKILPQAKLSGSLFQAGLPHKHKLFC"
     gene            complement(5060..6247)
                     /locus_tag="PLA58_04860"
     CDS             complement(5060..6247)
                     /locus_tag="PLA58_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62157.1"
                     /translation="MTEYDYWKIFPRMPKKVTIGDITIRDGFQHCEKFISTQAKIFYG
                     QEMILAGCRELEVTNLGNPRAMPQFKDADEVMKAMRGDDFRKRCGKAGIDVDKDVTLT
                     CVTIREAAVDRAIEMRKNGYGPDRILMMVSTDPEHHFANSGTTLSQYWREAERCIQKA
                     RDAGIKMCGTVSTIWGSPISGATKLEDAVEFTRRWFQIGAHDIEHADHDGSANAADAY
                     RYFSMVLDAMPNPEAHLCHLHETKRIASSTILAALQAGICRFEATLGGLGGQPANFLD
                     DCPIKGTGEYYYDPRYVGLICMEDLVVQIDELGIRHGYDVNRVLMLGRKMEKTIGARL
                     RSEAIINGRTVKEGHMEHARPGLKKLIEKEKEKPGQQFPAEWSAEAILPEKWGPADPL
                     WKK"
     gene            complement(6281..7078)
                     /locus_tag="PLA58_04865"
     CDS             complement(6281..7078)
                     /locus_tag="PLA58_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOR62158.1"
                     /translation="MADVLLREQKGEGITLLTLNRPEAMNCFNFDLLAALGDAVREAN
                     FDPGLRCLIITGAPADNPKKASFSTGADLIERRTMSPDQVRRFIFTIRNLFTSVEQLR
                     VPVIAAVNGFAFGGGTELALACDLRIAASNVVMGLTETSLAIIPGAGGTQRLPRIVGL
                     AKAKELIYTARRINAQTALDIGLVNRVVEPEKLIEAACELAREIAANGPVAVAQAKFA
                     INCGMDAPLSVALPLESKAYEITIPTKDRLEALAAFAEKRKPNFKGE"
     gene            complement(7195..8868)
                     /locus_tag="PLA58_04870"
     CDS             complement(7195..8868)
                     /locus_tag="PLA58_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOR62159.1"
                     /translation="MAEETRRMTRTERLEKERETNLKFWEEQDALLMKHRQEAYHIGG
                     EDQIARLAKQGKKPMRDLIQMLIDPGTEFFEVGLDAGYQIGEKKLYGGSIYEEEKRGH
                     IPGGGVVTGIGAIHGKDCMIFSNENRFAAGTYFPITLKKHMRAQAIAEQCGLPCIYIA
                     DSGGINLPLQLGCFADDGHFGTMFYNMCRMSAKGIKQYTLSTGGNTAGGAYMVYLATE
                     SIMIEKLAFAFLAGPPMVKSAIGETVSQEDLGGAYVHTQHSGGCDHFVQSQEEGIRKL
                     RAMMEFEPSQKLYIDRRESREPKFDYREMMRCSPVDTYHLINIKDTIKCLADDSYFHE
                     YKSTYGPGRGSSIICGKMWLKGIPVGVIASNANGVIYIEAARKATEWMIRCGNSKLPV
                     LFLQGSPGYMVGKAEEWGGIGKYGSDMVRACSCLEAPKITYVIGPDHGAANYGMCGRA
                     FKPRFAFTNMRGRTSVMSGNTAGFILETVRRKNIEAKGGAVNEEDMEKFRQMMRERYN
                     NEGHPFYTGSLMFHDGVIAFSEARDKLARAFEIALRAPVRESDWGDLKF"
     gene            complement(8923..10107)
                     /locus_tag="PLA58_04875"
     CDS             complement(8923..10107)
                     /locus_tag="PLA58_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR62160.1"
                     /translation="MDFWLKDEQKMLRYNIREFAEQEIAPVAMELDEKEEFSYDLTRK
                     MAKLGLFGIYVPAEYGGSGMDYLSYIVAVEEICRIDGSHGATVAAGNSLGIGPIYYYG
                     SKKQKDQWLPRLCSGEILASFGLTEPNAGSDAGASKTNARLENGQWVVNGSKIFITNS
                     TSKMAGICTVQAITGSKGQKVGPYGTIETKEVSCILVPNGTPGFTTKKMTKKMMWRSS
                     DTGELYFDDCRVPEENLLGKRGEGFHQMLQTLDNGRLAIAAMGLGGAQGAYELAMKYA
                     KERKQFGKPISTFQVNAFKLADMATEIEAARNLLYKACYLKDQGKSFGKEAAMAKLYC
                     SEVMGRVTNEALQIHGGYGLMKEYNIERFFRDYKLLTIGEGTSEVQRIVISRRIGCYE
                     TD"
     gene            10401..12572
                     /locus_tag="PLA58_04880"
     CDS             10401..12572
                     /locus_tag="PLA58_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOR62161.1"
                     /translation="MVPKETNNVTILCIDDDPDVLNLLQKILTGAGYSVIVAGDGKKG
                     IDEAQSRRPVLILMDVMMPEIDGYVACFELQANPETKFIPVIFLTALDDEVNRAKAFA
                     VGAADHLAKPIRKDILLKKIDEQIKTGSRWQKLKTRIAAKGEIVQPPAIFSQFKQSLL
                     NFVEPAQAARIRDRLSALAPQDIYNICEPMGVPQRQMTGWISEFLSIPIIAKIDRESL
                     LLGVIPVSFARENRVVARLDEKNERSFILCNPFDWSLMDALKKLFGLKSNAGLSLAAP
                     EDIDALFQQSEVETLMKLPGETQTESAEKMLAIDEAEQQEDEGEITQEINSEVIALVN
                     RILVDAYNKRASDIHFDSGFRGQPFKVRFRIDGVCQSAYFIPEKSKRAIISRIKIISN
                     LDISEHRKPQSGKFMVRVRGEKVEFRVEVTPTVENNENIVLRILAKSKPVTIRELGLS
                     THNLEAFENMITKPQGVVLCVGPTGSGKTTTLHSALGHINKPEITIWTAEDPVEIQQP
                     GLKQVQVNSKIGFTFSEALRSFLRADPDVIMIGEMRDAETAKITLEASLTGHLVFSTL
                     HTNSAAETVTRIIDMGIPVYNFADALLGIIAQRLTRRLCEKCKKPYHPDFEEYENLIH
                     YYDPVWAKKHGLPEYSPDFTLWMRQGCSYCEGKGYYGRVGIHEIITATAAVKKAIKQS
                     PDVDDLTQIALQEGMRTLRMDGVTKILQGITDYEQVSRVCL"
     gene            complement(12636..12965)
                     /locus_tag="PLA58_04885"
     CDS             complement(12636..12965)
                     /locus_tag="PLA58_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOR62162.1"
                     /translation="MADKEQDAPKGKFIVIVDEDLQDLVPGYLENRRKDVEELRRALE
                     RSDFEAIRVLGHKMKGSGGGYGFDEISEIGHALEDAAKQSQAAKIREEIDRLRYYLDN
                     VEVMMPS"
     gene            complement(12990..13580)
                     /locus_tag="PLA58_04890"
     CDS             complement(12990..13580)
                     /locus_tag="PLA58_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOR62163.1"
                     /translation="MPDKREKHILITGFGGQGIVMAGDILGKAATLYDHKHATMTQNY
                     GPEARGGACSSQVIISSEEILFPCVEEPEILVCMSQEAYTKNIKSLRPEGTLIWDTDL
                     VRTRKTDAVFKAFNIPATRFAEQLGTKMMANIVMLGFLSAVEPLVHAEALKKAVLESV
                     PAATRDNNLRAFNTGREYGHSILKGLVKPEPGKHQD"
     gene            complement(13586..14470)
                     /locus_tag="PLA58_04895"
     CDS             complement(13586..14470)
                     /locus_tag="PLA58_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOR62164.1"
                     /translation="MTTAKAVKNVPPHPMDGLLRTERIPHIWCPGCGIGTVFSAVLAA
                     IKNTGWDPNTIAMVSGIGCTGRMAGYIRLDSFHTTHGRALPFATGLKLAKPDTKVIIV
                     SGDGDLFAIGGNHFIHAARRNMDMTVVCVNNFNYGMTGGQQAPSTPLGAKTTTTVNGS
                     PEEPFNLCDLAATCGATYVARWTAMQVRSLRSSISEALEKRGFSFVEVITPCPSSFGR
                     RNRMGTALEMMKFYQGRSVIRSDIDPSEAVVDIDREIVVGKFVDIERPTFMDHYEKVN
                     RLNLPQWRAMHPDHQKGR"
     gene            complement(14532..15692)
                     /locus_tag="PLA58_04900"
     CDS             complement(14532..15692)
                     /locus_tag="PLA58_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOR62165.1"
                     /translation="MTAKEVRTGTHFMNGDEACCEAALAAGCRFFAGYPITPATEVAE
                     SMSRRLPEIGGIYIQMEDEIASMAAILGASWGGVKSMTSTSGPGFSLMMENIGLGICT
                     ETPCVVCNVQRAGPSTGLPTLVAQGDMMQARWGSHGHYEIIALSPSSPQEMFDFTIRA
                     FNLSEYYRLPVILLADETIGHMNERVVIPQEEDIEIVNRRKPTVSREDYLPYEADADG
                     IAPMANCGDGYRIHVTGLTHDDRGYPAMDAKAQEKMISRLVDKIRKNRDKIIRTQNLY
                     LDDAEVVVVSYGISARSAMQAVRDARNEGIKAGLIKLDTVWPFPEELIRSVAPRTRAM
                     IMAEINGGQMALELERSAGGACPVSLVSNLGGKILHPGLILNAVAKAATEKP"
     gene            complement(15713..15997)
                     /locus_tag="PLA58_04905"
     CDS             complement(15713..15997)
                     /locus_tag="PLA58_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR62166.1"
                     /translation="MQKTKKYPISQTDEIGRRKRKMGQVIVIEDRCKGCGFCIANCPR
                     QVMRLSSAFNKKGYHPPEVVDTTRCVSCHFCEIICPEFAIYSVEYEDFEE"
     gene            16371..17027
                     /gene="nth"
                     /locus_tag="PLA58_04910"
     CDS             16371..17027
                     /gene="nth"
                     /locus_tag="PLA58_04910"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOR62167.1"
                     /translation="MKDENIRPIVRILKKELDLGEMPIVSHLAESERDPFVILMSTLL
                     SLRTKDEVTAEATDRLLALAVTPEAMLKVPQSKIAKAIYPVGFYRNKAKTIHHVCREL
                     IERFGSKVPDNLDDLLSIKGVGRKTANLVITLAYGKDGICVDTHVHRISNRLGYVKTK
                     TPDETEMALRGKLPRPYWIMYNTLLVFFGRKICRPVSPVCSACPVSRYCDRVGVTVSR
                     "
     gene            complement(17093..17338)
                     /locus_tag="PLA58_04915"
     CDS             complement(17093..17338)
                     /locus_tag="PLA58_04915"
                     /inference="COORDINATES: protein motif:HMM:NF018500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HOR62168.1"
                     /translation="MAIHIIPVHKLSAKALKGVIEEFISRNGTDYGAVEASLETSFQQ
                     VKNRLKNGSAVLVFDDETETTNIFPADDPVLKKLDTQ"
     gene            complement(17371..18528)
                     /locus_tag="PLA58_04920"
     CDS             complement(17371..18528)
                     /locus_tag="PLA58_04920"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOR62169.1"
                     /translation="MQRFCSIFSQLLQLFPRLEFQSAVTATKAERHMRGFTCRGQFVS
                     MLFCQLGRAHSLREITNGLRSCEGKLKHLGIEAPQRSTLAYANEHRSWKLYEKVFFNL
                     LETCRQQVKGKKKFRFKNKWVSLDSTTMDLCLSMYDWAKFRRAKGAVKLHLVLDHDGY
                     LPCFAHITDGKCHDVKVAHTLRFESGTIVVDDRGYNDYKLFSKWTEQGVYFVTRMKDN
                     AVYEVTETREIPQNRSILSDEIIQLTGEAGKKCPCLLRRVTYYNADKDETFVYLTNHL
                     KLGATTIVSIYKDRWQMELFFKALKQNLKIKTFVGTSANAVKIQIWTALIVMLMLRYL
                     QLKASFGWSLSNLVAILRMNLFVHRDLWTWLDNPYEGPPLSTMQPQAKLQF"
     gene            18892..>20207
                     /locus_tag="PLA58_04925"
     CDS             18892..>20207
                     /locus_tag="PLA58_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOR62170.1"
                     /translation="MTNGKRVKAQAAEEKTVQTADGAPEKYFVVDTSALEYGMDVFEK
                     LRQGGRNCVLIPYATWEELDSHKTGNDFKADIARSVIRNVRNLFRVKDRSVRFFSYDF
                     SAAKDADLKIDKNDHCIIATALYARQKLPAEKVILVTQDAALETLALNFGMDVQSVKM
                     DETEVKQQHTPRTTVNLQKKWIADKTFPLKLPEARLAAQLGRILLNEGIVCYCERDGA
                     WGPAFAAVRKGDYFRIVDKDIHLLGVRPRSNNGDVNWGQYIAMDLLRDTSVPLVALTG
                     KAGTAKTFLALLAAFEQASRYRQILVSRATIQLGNKDRLGYSPGDIDAKMKPWMHPIH
                     DNINAIKELVDEKMKDKINEWLTNGKIEMMDLDKIRGRSIQKCFVIVDEIQNLPPSHV
                     KAIATRGGEGTKMVFTGDFSHDQIDVPWLDERSNGLSVLNAKMAGSR"
BASE COUNT         4319 a         5505 c         5584 g         4799 t
ORIGIN      
        1 gcgattcagc atacggccgg cctgggagaa gcggatttta tcgtggcgat caacaagaat
       61 ccccaggcga ccatgatgaa gatggccgac gtcgccgtcg tggccgacgc gggggactgg
      121 ctcaaccacc tgatcaggga actgaaaaag cgcattcggg aataagtgct tcccggacgg
      181 gaaaggaggc gcgatggagt tgctggggct catggaaaaa gtctatctgg gcaatacgct
      241 gcaaaactgg atcatcggcg tctgcgtcct ggtgggcgta ttttgcgcgc tgaaaattct
      301 gcaacgcatt gccattgcca ggctttccaa gctggcggcg gccacggaca accagattga
      361 cgaccttctg gtcagcatgc tggggcagac caagtttctg atcctgctgg tggcttccgc
      421 gtatgtggcg tcccaggtga tcaccatcaa accgtctgtc gaggcggtat ggcaaaagat
      481 tgtcggcctg attgtgatca ttcagttcgg actgtgggcg agcgccggcg ttacctttgc
      541 cttaggccgg gcgattgaaa agcggatgga ccgggatgcg gggagcgcca caaccctgac
      601 tctgctgggc ttcgtcgcgc ggctgatttt gtgggtgatt gtgctgctgt tgatcctgga
      661 caacctgggc gtcaacatca ccggcctggt ggcggggctc ggcatcggcg gcatcgccgt
      721 ggcgctggcg gttcagaata ttctgggcga tcttctggcc tctttgtcca ttgttctgga
      781 caagcccttt gtcatcggtg attttattgt cgtggattcc ctgtccggca cggtcgagca
      841 tatcggactc aagacgacgc gcatccgcag cctcagcggc gaacagctcg tctttgccaa
      901 caacgatctt ttgaagagcc gcatccgcaa ttacaagcgc atgcaggagc gccgtattgt
      961 tttcggcttc ggcgtggtgt attccactcc gctggaaaag ctgacgctca tcaaaaagat
     1021 ggttggcgac attgtcagtg gaacggaaaa cgcccgtctg gaccgcattc atttcaagga
     1081 atacggcagt tcttctctga attttgaggt cgtttatttt gtggctaacc cggatttcgg
     1141 cctctatatg gacattcagg aggcgatcaa tctggaaatt ttccggcgct ttgccgaaga
     1201 gggcattgaa ttcgcctatc cgacgcagac gatttacgtc catcatcctt aaagagaatg
     1261 cgaggacgat ttgaaaccta acggatggat tttgctttca gtgtgcgcgg cgtcgatttt
     1321 ttccccggat ctgtgtcttg ccgccggggc cgcccccaat atcggcgccg ttctgccgtt
     1381 gtggtcgatg ctgccctttg cgggtatttt gctttcgatt gcgctcgttc ccctcgttct
     1441 gccccgtttc tggcatcgcc attttcccaa ggtttccgct ttctgggcga ttgtctttgc
     1501 cgttccgttc atttattttt tccgcgaaac cgctttgtat gaaatcggcc acattatgat
     1561 cgtggactat atccccttta ttctgcttct gtggtctttg ttcacggttg caggcggcat
     1621 ctatatcaag ggcacgctga agggaacccc cgccgtcaat gtcatgattc tgatcatcgg
     1681 aacgcttctg gcgtcggtca tcggcacgac cggcgcgtcc atgctgctca tccggcctat
     1741 cctgcgatcg aacgcctggc gggtgtataa ggttcatacc attgtgtttt ttatctttct
     1801 ggtcagtaat atcggcggct ccctgacgcc gctgggggat ccgcccctgt ttttggggtt
     1861 tctgcacggc gtcccttttt tctggaccat gaacattctg ccggaaatgg cgtttgccag
     1921 cattattctt ctgattttgt attttcttct ggattcttac tattttcgaa aggaaagcaa
     1981 gaccgccgtg ccggaagatg agtcagagcc gctcggcatc gaggggaagc gcaatttcat
     2041 tttcctcatc ggcgtgatcg gagcggtttt gttcagcggc tccgtcaagc tgggcgagat
     2101 cagcgtcttc ggcattcacc agaccgtgga aaatttgatc aaggacgcag tcctgatcgc
     2161 catgggaatc ctttcgcttt ggcttaccgg aagggatgtg cgcaaaggaa atgaattcac
     2221 ctgggcgccc attttggaag ttgcctatct gtttgccggc attttcatca ccatcatccc
     2281 ggcgctggcg attctgaaag cgggcgagca gggagctctg gcgtggatga tccggtcggt
     2341 caattcgccg gccgaatatt tctgggcggt gggcctttta tccagttttc tggataacgc
     2401 cccgacttat ttgacctttt tcaacagcgc gctcggtaag ttctatccgg gcattccgga
     2461 agctcaggcg gttgccggcc tgattgttga acaaattccc tacctggccg ccatttccgc
     2521 cggagcggta ttcatgggcg ccaacaccta tatcggcaac gctcccaatt tcatggtgaa
     2581 atccatcgcg gaagaggctg gtgtcaaaat gcccagcttt ttcggataca tgttcaaata
     2641 ttccatcccg attctggttg ttttgttcat cgtcatcacc tttattttct tttgatgttt
     2701 gacaaatcca caggcgtata aatattgctt gaagatggcg tgtaaagcgg ataaggtgaa
     2761 atcagctttg catgacgttg ctgcaaccga accgccgccg gaaatgataa agctggaaaa
     2821 agaggaatat ggaggacagt tatgggttca accctgcgca aatctttaaa aacgattgag
     2881 gaatacaaaa ccgccaatcc gcattacacg gatctgctgg atatccttgg ggatatcctg
     2941 attctgcgtg aagaataccg caagaaaatg acgaagccga tcttttcagt ggaggacaag
     3001 ttgatccccg gaaaaatggc gggaggcctt cctctgattg atcttgcggg gaacaagtat
     3061 gatttgaccc gaccgaagga atattttgac tcgctgatat ccattgccca aaagagaatg
     3121 cctgccgaag cacaccggtt tctggatgtg atcaaaaatg agcagttcga ctgggaggcg
     3181 atcattcgag aatcttttaa tcccgccccc caaagcgaag atgttccgga aagggaagcc
     3241 agggcatccg gcgagcatga tgagcagatt gacctgatcg atttgttcat cgaagaaagc
     3301 cttcgtccgg aactggaagt ggtcgccgaa aaatatggcg ctctggtgga gaaatcggac
     3361 tggacggaag gctattgtcc gatctgcgga aaggagccga aaatcggcga aatccgggaa
     3421 agcgaagacg gaaggcgtta tctcttttgc aaccagtgcg gttataaatg gcacttccgc
     3481 cggatcaaat gccccttctg cggaaatgaa gaacagcact cgctggcgta ttttgccgtg
     3541 gagggagagg aaagccaccg ggtggacgtc tgcaacaagt gccgccgtta cattaaaatt
     3601 attgagctgc cgaagtcgtc cgagggagtg aatctggacg tggaagatat cgccacgctg
     3661 catctggata tgctggcgta cgaagaagga tataattagg ctgaagaccg aaggctgaag
     3721 gatgagggaa caaagaatcc ctttgggaca gcgcatccca aagggattct ttgttgcgtt
     3781 ctcccttgaa tgccgagaga cctttgaaaa atttcatttc gaggagcgta atcctgctcc
     3841 tgatgtcatt tcgaaggagc acagcgactg agaaatctta agatccctcg agacgtttgc
     3901 ataaccacat aaaaacaaca atattgtcat tcctcgcgaa gcctgccatc gagaagtcgg
     3961 ggggcgggaa tccaggatta aaggaactgg ttccccgcct ccgacgaata agagtagttt
     4021 ttcaaagctc tcgccgagga agtggttcag cagaaaagtt tgtgcttgtg gggcagaccg
     4081 gcttggaaaa gggagccgga gagtttcgcc tgcggcagga ttttttgcag ccaggcgcag
     4141 acgttgagga gttttttcag atccattcct gttttgatgc ccatggattc aaacatgaac
     4201 accaggtctt ccgtggcgac attgccggaa gacccgggcg cgtaagggca tcctcccagc
     4261 cccccggcgg acgaatcgaa ggatcgcact ccgatatcgt aggcgcgcag ggcattggcc
     4321 tgcgccagcc cccgcgtgtt gtgaaaatgg gcgccgaagc gaatttcagg aaaacggcgg
     4381 aagcaggcga ggaacttttc ttctacgtct ttcggatggc cgaaccccac cgtgtcgcag
     4441 agcgtgattt cccggacgcc cagagcggca actttttcca cggtgcgcaa aacggcagcc
     4501 gtcgtgacat tgccggaaaa cgggcagccg aaggccgtgg ccagatccac ccggagagtc
     4561 attgaaggat tgctcaggtg tgaccggcag atgtgctgca actccttcag ggagtcaagc
     4621 ggcgattgtt gtacgttggc gtaattgtgt tttgaactca cggagaagaa gaagatcagc
     4681 ctggcaatgc cgcaggccag cgctttgtcc gccccgcgcc cgttggggac cagcgccgac
     4741 ggcacgaaac ccttcaagcg gcggacggct tgggcgactt cctccatatc ccggaactgt
     4801 gcgatggcct gcggagagac gaaagagccg atctggattt cctgcgcgcc tgtgccggcc
     4861 agttttttga tcagggcgat tttttggtcc gtcgggacaa acgcctgaag gttctggagg
     4921 ccgtcgcgcg gggcgacttc aacaattttt gcttttttca tggcgcgctt ccatttcatt
     4981 cctgacggac agtgaaagct aacaaaaaag gaagctcacc gcaaggtttt gggtgagctt
     5041 cctttttttg atgaagggct tactttttcc agagggggtc cgccggtccc cacttttcgg
     5101 gaaggatcgc ttcggcgctc cactccgccg gaaactgctg gcccggtttt tccttttctt
     5161 tttcaatcag tttcttcagg ccggggcgcg cgtgctccat gtgcccttcc ttgacggtgc
     5221 ggccgttgat gatcgcttcg gagcgcagtc tggcgccgat ggtcttttcc atttttctgc
     5281 ccagcatcag gacgcggttg acgtcgtatc cgtgccggat gcccagttcg tcgatctgca
     5341 cgaccaggtc ctccatgcag atcaggccga cgtagcgcgg atcgtagtaa tattcccccg
     5401 tgcccttgat cgggcagtca tccaggaaat tggccggctg gccgcccaag ccccccagcg
     5461 tggcctcgaa gcggcaaatg ccggcctgca gggccgcgag aatggtggaa gaggcgatcc
     5521 gcttggtttc atggagatgg cacagatgcg cttccgggtt gggcatggcg tccaacacca
     5581 tggaaaaata ccggtaggca tcggctgcgt tggcggagcc gtcgtgatcg gcgtgttcga
     5641 tgtcgtgagc tccgatctgg aaccatcgcc tcgtgaattc caccgcgtct tccagtttgg
     5701 tggcgccgct gatcggagat ccccagatgg tgctcaccgt gccgcacatt ttgatgcccg
     5761 cgtctcgcgc cttctgaatg catcgttcgg cttcccgcca gtactggctc agcgtggttc
     5821 cggagttggc gaaatgatgc tccgggtcgg tcgaaaccat catcaggatc cggtcgggtc
     5881 cgtagccgtt ttttcgcatt tcaatggccc ggtccacggc ggcttcccgg atggtcacgc
     5941 aggtgagcgt gacgtctttg tccacatcga tgccggcttt gccgcagcgt ttccggaagt
     6001 cgtcgccgcg catggccttc atgacttcgt ccgcgtcctt gaactggggc atggccctgg
     6061 ggtttcccag gttggtgact tccagctccc gacagccggc aaggatcatt tcctgcccgt
     6121 agaatatttt cgcctgtgtg gagatgaact tttcgcaatg ctggaaaccg tctcgaatgg
     6181 tgatgtctcc aatggttact ttcttcggca tccggggaaa aattttccag taatcatact
     6241 cggtcataca tcattctcct tttgtcgatt tgtttacggt ttattcacct ttgaaatttg
     6301 gtttcctttt ttcggcaaag gcggccagcg cctccagccg gtccttggtc gggatggtga
     6361 tttcatacgc cttcgattcc agcggcaggg cgaccgacag cggcgcgtcc attccgcaat
     6421 tgatggcgaa tttggcctgc gccacggcca ccgggccgtt cgcggcgatt tcacgcgcca
     6481 gttcgcaggc ggcttcgatc agtttttccg gctccacgac ccggttgacc agcccgatgt
     6541 ccagcgcggt ctgggcgttg atgcggcggg cggtgtagat cagctccttg gccttggcca
     6601 ggccgacaat gcgcggcagc cgctgcgtgc cgcccgcccc cggaatgatc gcgagcgaag
     6661 tttccgtcag ccccatcacc acgttgcttg cggcgatgcg cagatcgcag gcgagcgcca
     6721 gctccgtccc tccgccgaag gcaaagccat tgacggccgc gatgaccgga acgcgcagct
     6781 gctccacgga cgtgaagaga ttgcggatcg taaaaatgaa gcggcgcacc tggtccgggg
     6841 acatcgttcg ccgctcgatg agatcggcgc cggtggagaa agaggccttt ttggggttgt
     6901 cggcaggcgc tcccgtgatg atcagacacc gcaggcccgg atcgaaattg gcttcccgga
     6961 cggcgtcgcc cagcgcggcc agcaggtcaa agttaaaaca gttcatggct tccggacggt
     7021 ttaacgtcag aagcgttatc ccttcgcctt tttgttcccg taataaaaca tctgccatag
     7081 gtcggcctcc ttaatgtgaa ccgtttgata tgaacatgcc ccctccccct tgacggagga
     7141 gggaagagca ggggtgatcc tcatccctcg ttgtgcccgc cacgggcatg ggggttagaa
     7201 tttcaaatcc ccccagtcgg attcccggac cggcgcgcgc agcgcgattt caaaggcgcg
     7261 ggccagtttg tcgcgcgctt cggaaaaggc aatcacgccg tcgtgaaaca tcagcgagcc
     7321 ggtgtagaag ggatggcctt cgttgttgta gcgttcccgc atcatctgac ggaatttttc
     7381 catgtcctcc tcgttgacgg cgccgccttt ggcttcaata ttcttgcggc gcaccgtctc
     7441 cagaatgaac ccggccgtgt tgccggacat cacggacgtt cttccccgca tgttggtgaa
     7501 ggcgaaccgc ggcttgaacg cccggccgca catgccgtag ttggccgcgc cgtgatccgg
     7561 tccgatgacg tacgtgattt tcggcgcttc caggcaggag caggcccgga ccatgtcgga
     7621 gccgtatttg ccgatgccgc cccattcttc ggccttgccg accatgtagc cgggcgatcc
     7681 ctgcaggaac aaaacgggaa gtttggaatt gccgcagcgg atcatccatt ccgtcgcttt
     7741 gcgggccgcc tcgatataaa tgacgccgtt ggcgttggag gcgatcacgc cgacggggat
     7801 tcccttgagc cacattttgc cgcagatgat cgagctgccg cggcccggtc cgtacgtcga
     7861 tttgtattca tggaaatagc tgtcgtccgc caggcatttg atcgtgtcct tgatgttgat
     7921 caggtgatac gtatccacgg ggctgcagcg catcatttcc ctgtaatcga atttgggttc
     7981 gcgggattcc cggcggtcaa tgtagagctt ctgcgacggt tcaaattcca tcatggcgcg
     8041 cagcttccgg atgccttcct cctgcgactg cacgaaatga tcgcacccgc cggaatgctg
     8101 ggtgtgcacg tacgcgccgc ccagatcttc ctgggaaacg gtttcgccga tggcggactt
     8161 gaccatgggc gggccggcga ggaaggcaaa cgccagcttt tcaatcatga tcgattccgt
     8221 ggccagatac accatgtagg cgccgccggc cgtgttgccg ccggtcgaaa gcgtgtactg
     8281 cttgatgccc ttggccgaca tgcggcacat gttgtaaaac atggttccga aatgaccgtc
     8341 gtcggcaaag cagcccagtt gcagcggcag gttgattccg ccggagtcgg caatgtaaat
     8401 gcaggggagg ccgcactgtt ccgcgatggc ctgcgcgcgc atatgcttct tcagggtgat
     8461 cgggaaatac gtgccggcgg caaagcggtt ttcgttggag aaaatcatgc agtcttttcc
     8521 gtgaatggcg ccgattcccg tgacgacgcc gccgcccggg atgtgcccgc gtttttcctc
     8581 ttcataaatg gagccgccgt agagtttttt ttcgccgatc tggtagcctg cgtccagccc
     8641 cacctcaaaa aattccgtgc ctggatcaat cagcatttga atcaggtccc gcatcggctt
     8701 tttgccctgc ttggccagcc tcgcaatctg gtcttccccg ccgatgtggt aggcttcctg
     8761 ccggtgcttc attaaaagag cgtcctgctc ctcccagaat ttcagattgg tttcccgctc
     8821 tttttccagc cgttccgttc tggtcattcg cctggtttct tccgccataa ttcttcctcc
     8881 ccaaaagata aacgtttcgc cgtggaatcc ggagattcac tcttaatccg tctcgtagca
     8941 gccgattctt cgtgaaatga cgatgcgctg cacttcagag gttccctcgc cgatggtcag
     9001 gagtttgtaa tccctgaaaa accgttcaat gttgtattcc ttcatcaggc cgtagccgcc
     9061 gtgaatctgc agggcttcgt tggtcacgcg gcccatgact tccgagcaat aaagcttggc
     9121 catggccgct tcttttccaa aagacttgcc ctgatctttc aggtagcagg ccttgtataa
     9181 taaattgcgc gcggcttcga tttccgtggc catgtcggcc agtttgaacg cgttgacctg
     9241 gaaggtggag atcggtttcc cgaactgttt cctttctttg gcgtatttca tggccagttc
     9301 ataggcgccc tgcgcgcccc ccagtcccat ggcggcaatg gccaggcgtc cgttgtccag
     9361 cgtctggagc atctggtgaa atccttcgcc gcgctttccc agcagatttt cctccggcac
     9421 ccggcagtcg tcgaaataca gttcgccggt atcggaggag cgccacatca ttttcttcgt
     9481 catctttttt gtcgtgaaac cgggcgttcc gttcgggacg aggatgcagg acacttcctt
     9541 ggtttcaatt gtgccgtagg ggccgacctt ctgtcctttg gatccggtga tggcctgcac
     9601 cgtgcagatg ccggccattt tgctggtgga attggtgatg aagattttgc tgccgttcac
     9661 cacccattgt ccgttttcca gccgcgcgtt ggtcttgctg gcgcccgcat cggagccggc
     9721 gttgggttcg gtgagcccga aagacgccaa tatttcaccg gagcagaggc gcggcagcca
     9781 ttggtccttc tgctttttgc tgccgtaata ataaatcggt ccgatgccca gggaatttcc
     9841 ggcggcaacc gtcgcgccgt gtgaaccgtc gatgcggcag atttcctcaa ccgcgacgat
     9901 gtaggaaaga taatccatgc ctgatccgcc gtattcggcg ggcacataaa tgccgaaaag
     9961 accaagcttg gccattttcc gggtcaggtc ataggaaaat tcctcttttt catccaactc
    10021 cattgcgacc ggcgcgattt cctgttcggc gaattcccga atgttgtagc gaagcatttt
    10081 ctgttcgtcc ttcagccaga aatccatggt agccctccaa ctacttgtat tttttaaatt
    10141 taattaatgt aaaggttccc ctcatgattc cgttaaaaaa ttcacccagg cgtcggggag
    10201 ttgacgatcc gacgttgtgc aaggattgga tggatgatcc atattttgat tggacagtca
    10261 accaataatt ttcaaatact ctagcgaggg gagaattgtc aaggggaaat ttttatttcc
    10321 tgacggttat ggaatcggga cattctttct tcttgacaag aaatcaccct atcaggttat
    10381 acgttttcca aggaggcaaa atggtcccga aagaaacgaa caatgtaacg atcctttgta
    10441 ttgatgacga tccggatgtt ctgaatctgc tccagaagat tttgaccggg gccggctatt
    10501 cggtcatcgt tgccggggat ggaaagaagg ggattgatga agcccagagc cggcggccgg
    10561 ttctgattct gatggacgtg atgatgccgg aaatcgacgg ctacgtggcc tgttttgaac
    10621 tgcaggccaa tccggaaacc aagttcattc ccgtgatttt cctgacggcc ttggatgatg
    10681 aagtaaaccg ggccaaagcc tttgcagtgg gcgcggcgga ccacttggcc aagccgattc
    10741 gaaaagacat tttgctcaag aagattgacg agcagatcaa aacaggttcc cgctggcaga
    10801 agttgaagac aaggattgcc gccaaggggg agattgtgca gccgccggcc attttttccc
    10861 agttcaagca atccttgctg aactttgtgg agcctgctca ggccgcccgc attcgggacc
    10921 gtctgtccgc gctggcgccc caggacatct acaatatctg tgagccgatg ggagttcccc
    10981 agaggcagat gacggggtgg atttcggaat ttctgtccat acccatcatc gctaaaattg
    11041 accgggaaag ccttctgctc ggggtcattc ccgtttcttt cgcccgggaa aaccgggttg
    11101 tggccaggct cgacgagaag aacgaaagat cgttcattct gtgcaacccc tttgactgga
    11161 gcctgatgga tgcgctcaag aaattgttcg gtctgaaaag caacgcgggg ctcagtctgg
    11221 cggcgcccga ggacatcgat gctcttttcc agcagtcgga agtcgagact ctgatgaagc
    11281 ttcccggaga aacgcagacc gaatccgccg aaaaaatgct ggccatcgac gaggccgagc
    11341 agcaggaaga cgaaggggaa atcacccagg aaatcaactc cgaagtcatc gccctggtca
    11401 accggattct cgtggacgcc tacaacaagc gggcatcgga cattcatttc gattccggct
    11461 ttcggggaca gccgttcaaa gtccgcttcc ggatcgacgg ggtttgtcag tccgcgtatt
    11521 tcattccgga aaagtccaaa agagccatca tttccagaat caagatcatc tccaatctgg
    11581 atatttcgga gcaccgcaag ccgcagagcg gcaaattcat ggtgcgcgtc cgcggcgaga
    11641 aggtggaatt ccgcgtggaa gtgacgccga cggtggaaaa caacgaaaat atcgtcctga
    11701 gaatcctggc caagtcaaaa cccgtgacca tccgggagct cggactttct acccacaatc
    11761 tggaagcttt tgaaaatatg attacaaagc cgcagggcgt cgtgctgtgc gtcggtccca
    11821 ccggatccgg aaaaacgacc acgctgcatt cggccctcgg ccatatcaac aagccggaga
    11881 ttaccatctg gacggcggaa gatcccgtgg aaatccagca gcccggcctg aagcaggtgc
    11941 aggtgaacag caaaatcggc ttcacgttca gcgaagcgct ccggtctttc ctgcgggccg
    12001 atcccgacgt gatcatgatc ggcgaaatgc gcgacgcgga aaccgccaaa atcacgctgg
    12061 aagcgtcgct gaccggccac ctggttttca gcacgctgca tacgaacagc gcggcggaaa
    12121 ccgtaacgcg catcatcgat atgggaatcc cggtttataa ttttgccgac gctttgctgg
    12181 gcattatcgc tcagcgcctg acccggcggc tttgtgaaaa gtgcaaaaag ccttaccatc
    12241 ccgattttga agaatatgaa aatctcatcc attactacga tcccgtctgg gcgaaaaaac
    12301 acggacttcc tgaatattct ccggatttta ccctgtggat gcgccagggc tgcagttatt
    12361 gcgagggcaa gggatattat ggacgggtcg gcattcacga gattatcacg gcgacggcgg
    12421 cggtgaagaa agccatcaag caaagcccgg acgttgacga tttgacccaa atcgcgcttc
    12481 aggaaggcat gagaacgctg cggatggacg gggttacaaa aatcctgcag gggatcacgg
    12541 attacgagca ggtttcgagg gtgtgcctgt agcttgtgtg aggaaattcg ccttgcatgt
    12601 cagcaaggct ggaagatcgc gccgtggccg gtggtttacg atggcatcat cacttcgaca
    12661 ttgtccaggt aataccggag ccgatcgatc tcttcccgga ttttagcggc ctgcgattgc
    12721 ttggcggcgt cctcgagggc atggccgatt tcgctgattt catcaaatcc gtagcccccg
    12781 ccggatcctt tcattttatg gcccagtacc cgaatggctt caaagtcgct ccgctcgagc
    12841 gcgcggcgca gctcctcaac gtctttgcgc cggttttcca ggtatccggg aaccagatcc
    12901 tgcaggtctt catcaacgat gacgataaat ttcccttttg gagcatcctg ttccttgtcg
    12961 gccatttgtg aaggattcct ttcgcctttt taatcctgat gtttgcccgg ctcgggcttg
    13021 accagtcctt tcaggatgga atggccgtat tccctgcccg tgttgaaggc gcggaggttg
    13081 ttgtcccttg tcgcggcggg gacggattcc aggacggcct ttttgagcgc ttccgcatgg
    13141 accagcggct ccaccgcgga gagaaagccc agcatgacga tgttggccat catcttggtt
    13201 cctagctgct ccgcaaaacg cgtggccggg atgttgaacg ccttgaaaac cgcgtcggtt
    13261 ttgcgcgtgc gcaccaggtc cgtgtcccag atgagcgtcc cttccgggcg cagggatttg
    13321 atgttttttg tataggcttc ctggctcatg cagaccagga tttccggctc ctccacgcag
    13381 gggaacagaa tttcctcgct gctgatgatc acctggctgg aacaggcgcc gccccgggct
    13441 tccggcccgt agttctgcgt catcgtggcg tgtttatgat cgtagagcgt tgccgctttt
    13501 cccagaatgt ccccggccat gacaatgccc tgcccgccga aaccggtaat cagaatgtgt
    13561 ttctcgcgtt tgtccggcat ggtcttcacc gccctttctg atgatcagga tgcatggcgc
    13621 gccactgcgg caggttgagg cgattgactt tttcgtagtg gtccatgaac gtgggccgtt
    13681 ctatatcgac aaatttgccc accacgattt cccggtcgat gtccacgacg gcttcgctgg
    13741 ggtcgatatc gctgcggatc acggagcggc cctgatagaa tttcatcatt tccagcgcgg
    13801 tgcccatgcg gttgcgccgg ccgaaagagg acgggcaggg ggtaatcact tccacgaagc
    13861 tgaagccccg tttttccagc gcctcgctga tggagctgcg cagcgaacgg acctgcatgg
    13921 ccgtccaccg ggccacatag gtcgctccgc aggtggcggc cagatcgcac aggttgaaag
    13981 gctcttccgg cgagccgttg acggtggtgg tggtcttggc gcccagcggc gtgctgggag
    14041 cctgctggcc gcccgtcatg ccgtagttga aattattgac gcaaacgacc gtcatgtcca
    14101 tgttccggcg agcggcatga atgaaatgat tgcctccgat ggcaaacagg tcgccgtctc
    14161 cggagacgat gatcaccttg gtgtccggtt tggccagctt gagtccggtg gcaaacggaa
    14221 gcgcccggcc gtgtgtggta tgaaaagaat ccagccggat gtagccggcc atgcgccccg
    14281 tgcagccgat gccggaaacc atcgcgatgg tgttggggtc ccagccggtg tttttgatcg
    14341 cggcgagcac ggcggaaaac accgtgccga tgccgcagcc ggggcaccag atgtggggga
    14401 ttctttccgt ccgcagcaat ccatccatgg gatggggcgg aacattcttg acggcttttg
    14461 ctgtggtcat aggtcctgtc ctgcttcatc gaacaaggga ttgcagcccc ttgttcttcg
    14521 gttttgctga attatggttt ttctgttgcc gccttggcaa ccgcgttcaa aatcaggccc
    14581 gggtgaagga tcttgcctcc gagattggac accagcgaca ccgggcaggc gccgccggcc
    14641 gagcgttcca gttccagcgc catctggccg ccgttgattt ccgccatgat catcgccctg
    14701 gtccggggcg cgacgctgcg gatgagttct tcggggaaag gccagacggt atcgagtttg
    14761 atcaggcccg ccttgatgcc ttcgttgcgg gcgtcgcgga cggcctgcat ggcggagcgc
    14821 gcgctgattc cgtaggaaac caccacgact tcggcgtcgt ccagatacag gttttgcgtg
    14881 cggatgattt tatcccggtt ttttcgtatc ttgtccacga gccgcgaaat cattttttcc
    14941 tgcgctttgg catccatagc cggatagccc cggtcgtcgt gcgtcagtcc cgtgacgtga
    15001 atccggtagc cgtcgccgca attggccatc ggcgcgattc cgtcggcgtc cgcctcgtag
    15061 gggaggtaat cttcccggga aacggtcggc ttgcggcggt tgacgatttc aatgtcttcc
    15121 tcctgcggga tgacgacgcg ttcgttcatg tgtccgatcg tctcgtcggc cagaagaatg
    15181 acgggcaggc ggtagtattc gctcaggttg aaggcccgga tggtaaaatc gaacatttcc
    15241 tgcgggctcg acggagacag ggcgatgatt tcgtagtgcc cgtgcgagcc ccagcgcgcc
    15301 tgcatcatgt ccccctgggc caccagcgtc ggcagtcccg tgctggggcc ggcgcgctgc
    15361 acattgcaga ccacgcaggg cgtttccgtg cagatgccca gaccgatatt ttccatcatc
    15421 agcgaaaagc ccggacccga cgtgctggtc atcgatttca ctccgcccca gctcgccccc
    15481 agaatggccg ccatcgaagc gatttcgtct tccatctgga tataaatgcc gccgatttcc
    15541 ggcagccgac gcgacatgga ttcggccact tccgtggcgg gcgtgatcgg atatccggca
    15601 aaaaagcggc aacccgcggc cagcgcggct tcgcagcagg cctcgtcgcc gttcatgaaa
    15661 tgcgttcctg ttcttacttc tttagcagtc aaagcctctc tccttctacg gctcactctt
    15721 cgaaatcttc atattctacg ctgtaaatcg caaattccgg acaaataatt tcacaaaaat
    15781 ggcagcttac acagcgggtg gtgtccacca cttccggtgg atgataccct ttcttgttga
    15841 aagcagagga gaggcgcatc acctgccggg gacagttggc gatgcaaaaa ccgcatccct
    15901 tgcagcggtc ttcaatcacg atcacctgcc ccattttcct ttttcggcgg ccgatctcat
    15961 ccgtctgact gattggatat ttttttgttt tctgcatgaa tattcttatt ttcctcgcga
    16021 attcggcgct gtgcggagca ttccgccgga tggattgaat ttgcttgatg atcggggatg
    16081 atctctaaaa ccccaggtcc ctgaagtgcc aaactccatc ttgcgtgcgc gaaggcgcaa
    16141 actgggcatt ataggaataa gaaagcgctg tcaagaaaaa acacgccggt caaaacggac
    16201 ctttattaat aattctccat actccgagcg ctatgcccgg agaatttacg gataaaaact
    16261 tgcttttcaa caagtctcgt cataataccg ggaggcctcg aaccggtcat gaaccggcgc
    16321 cggaaagggg gccccaacag gaaatttcaa aaggaaagca gacgcaaaac atgaaagacg
    16381 aaaacatccg tccgatcgtc agaattctga agaaggaact ggatctgggg gagatgccca
    16441 tcgtttccca cctggccgaa agcgagcgcg atccgtttgt catcctgatg tccacgctct
    16501 tgagcctgcg caccaaagac gaggtcacgg ccgaggccac cgaccgtctt ctggcgcttg
    16561 ccgtgacgcc cgaggccatg ctgaaagttc cgcagtccaa aattgccaaa gccatttatc
    16621 cggtgggatt ttatcgcaac aaggccaaaa cgattcatca cgtctgccgg gagctgatcg
    16681 aacggttcgg ttcaaaagtg ccggacaacc tggacgacct gctgagcatc aagggcgtcg
    16741 gaaggaaaac ggccaatctg gtgattacgc tggcgtacgg gaaggacggc atttgcgtgg
    16801 acacgcacgt tcaccggatt tccaaccgtc tgggctacgt gaaaacaaag acaccagacg
    16861 aaacggaaat ggccctgcgc ggcaagcttc cccgtccata ctggattatg tacaatacgc
    16921 tcttggtgtt tttcggacgg aaaatctgca gacccgtctc gcccgtttgc agcgcctgtc
    16981 cggtttcccg ctactgcgac cgcgtggggg tgacggtaag ccggtgaggt caacggactc
    17041 tttctgtgcc gatggatatg attccatagt ttcctatatc ctcatcagcc ggctactgtg
    17101 tatccagctt tttcaaaaca ggatcatccg ccggaaatat attggtggtt tccgtctcgt
    17161 catcaaaaac aagaacggcc gatccgtttt tcagccggtt tttgacctgt tgaaaactgg
    17221 tttccaggga agcctcaacc gcgccgtaat cggttccatt tctggaaata aattcctcga
    17281 taaccccttt taaggcttta gcgctgagct tatggacggg aatgatatgg atcgccattt
    17341 ttatatcaac cttcactgct gtccaaaata tcagaattgt aatttcgcct gcggttgcat
    17401 ggtggataag ggcggtcctt cataaggatt atcaagccat gtccagagat cacgatgcac
    17461 aaagagattc atccggagaa tggccaccaa attggaaagc gaccagccaa aggatgcttt
    17521 caactgcagg taacgaagca tcagcatgac aatcaatgct gtccaaattt gaatcttcac
    17581 cgcattggcc gatgtgccta caaacgtttt aatcttaaga ttctgtttca atgctttaaa
    17641 aaacagctcc atctgccagc gatctttgta gatggaaaca atggtggttg cccccaactt
    17701 caaatgattg gtcagataaa cgaatgtctc atccttgtct gcattatagt aggtcaccct
    17761 gcgtaacagg catggacatt ttttgcccgc ttcaccggtc aattggatga tttcatcact
    17821 gagaatcgaa cggttttggg gaatctctct ggtttcggtt acttcataga ccgcattatc
    17881 cttcatccgg gttacaaaat agacaccctg ttccgtccat ttgctgaaaa gcttgtaatc
    17941 attatacccg cgatcatcca ccacgatggt tccggattca aaccttaatg tatgggcaac
    18001 tttcacatca tggcatttcc catccgtaat gtgagcaaaa caaggcaaat acccatcatg
    18061 atccaacacc aaatgaagtt ttacagcgcc cttggcccgc cgaaactttg cccagtcata
    18121 catcgaaaga cacaaatcca tggtggtcga atctaaactt acccatttgt tcttgaatcg
    18181 aaatttcttc ttgcccttaa cctgttgccg acatgtctcc aaaagattga aaaatacctt
    18241 ctcataaagc ttccaggatc gatgctcatt cgcataagcc aaagttgacc gctggggagc
    18301 ttcaatgcct aaatgtttca acttcccctc gcagctgcgc aatccgtttg tgatctcccg
    18361 caatgaatgc gctcgaccca attggcaaaa taacatgctt acaaactgac cccggcaggt
    18421 aaatccacgc atgtgacgct ccgccttggt tgctgtcact gcgctttgaa attccaatcg
    18481 tggaaataat tgtaacagct ggctgaatat gctacaaaat ctctgcatcg ttgagtcctc
    18541 cttgtctgta tttgctcgta gcaaagccaa tatagcaaat cagggctcaa catgcgctgt
    18601 tatttcttta cctaatttgg acaagagtga tgcataatgt atttctaatg gtttccattg
    18661 gaacatgtta cgttcgttct ttcaactact atcacgcgca aatcagttga ttcgatcaaa
    18721 atcatctaat gggaaatctg ggttgaaaaa agctgttcaa ttttcccttg tcgaaatttc
    18781 cgaatttggt acaatggcac acaacaattc tgccgacgca ggcattggag gtgcgccttc
    18841 acacattctg cattccataa cttcaaacac gtcaaccggg gagcgagggg catgacgaac
    18901 gggaaaagag tcaaggctca agcggcggaa gagaagacgg ttcagacggc ggacggggcg
    18961 ccggaaaaat attttgtcgt ggataccagc gccctggaat acgggatgga tgtttttgag
    19021 aagcttcgcc agggaggacg aaactgcgtt ttgattccct acgcgacctg ggaggaactg
    19081 gacagccaca aaaccggaaa cgatttcaaa gcggacattg cccgcagcgt cattcgcaat
    19141 gtccgcaatc tgtttcgggt caaagaccgt tcggtccgct ttttctccta cgacttcagc
    19201 gcggccaagg acgctgacct caaaatcgac aaaaacgacc actgcatcat cgctaccgcg
    19261 ctctacgccc ggcaaaagct gccggccgaa aaagtcatcc tcgtcacgca ggacgccgcg
    19321 ctggaaacgc tggccctgaa tttcggcatg gatgtccaat ccgtcaaaat ggatgaaacg
    19381 gaggtcaagc agcagcacac cccccggacc accgtcaatc ttcagaagaa atggattgcc
    19441 gataaaacct ttccgctgaa acttcccgaa gcccgactgg ccgcgcaact gggccggatc
    19501 ctgctcaacg agggcatcgt ctgctactgc gaacgcgacg gggcctgggg acccgccttc
    19561 gccgcggtgc gcaagggcga ctacttccgc atcgtggaca aggacattca tctgctgggc
    19621 gtaaggccca ggtccaacaa cggagacgtc aactggggcc agtatatcgc catggatctg
    19681 ctgcgcgaca catccgttcc gctggttgcg ctgacgggca aggcgggaac cgccaagacc
    19741 tttctggcgc ttctggccgc cttcgaacag gcttcccgct accgccagat tctggtcagc
    19801 cgcgcgacca tccagctggg caacaaagac cggctgggct attcaccggg cgatatcgac
    19861 gccaagatga agccctggat gcacccgatc cacgacaaca tcaacgcgat caaagaactg
    19921 gtggatgaaa aaatgaagga caagatcaac gagtggctga ccaacggaaa aatcgagatg
    19981 atggacctgg acaaaatccg gggccgctcc attcagaaat gcttcgtgat cgttgacgag
    20041 attcagaacc tgccgccttc gcatgtaaaa gccatcgcga cgcgcggcgg cgaaggcacc
    20101 aaaatggttt tcaccggcga cttttcccac gatcaaatcg acgtcccgtg gctggacgag
    20161 cgctccaacg ggctttccgt gctgaacgcc aaaatggccg ggagccg
//
LOCUS       DAOSRM010000034        20049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2868_length_20049_cov_111.256527, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000034 DAOSRM010000000
VERSION     DAOSRM010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 20049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20049
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2868_length_20049_cov_111.256527"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1361)
                     /locus_tag="PLA58_04930"
     CDS             complement(72..1361)
                     /locus_tag="PLA58_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOR62171.1"
                     /translation="MSIWQKKNRILITCPKGVVPYLKAEIEELGFPILQEIDTAVSTE
                     GTLSDTMVLNLHLRTAQHVLYQLQIFKAISAGAFYERLNAIPWETFLHDSGPSAYLSV
                     TSVVDHPEITDTRFANLRAKDAIVDRIRDKTGVRPDSGPERNRAVIHVYWRNDQAMAY
                     IDTSGERLSLRGYRKIPLAAPMQETLAAALIKATGWQGRTPLVNPMCGSGTLAIEAAL
                     MALHRAPGLGRGNYGFMHIRDFPHDSWQELRRKARNDSLKALPSTVIASDIDPQAVEA
                     ARRNAQTAGVDRLIEFKVCPFEKTPTPGGKGVIIINPPYGERLEPLKIKTAQSAPKKE
                     IAPGQKVIVRKAADLAHKRSSPSALQRLEALYEGCGDWLKGIGRGYHGYLFTGNLEMI
                     KKVGLRTKRRMTFYNGEIECRLLEYDLYEGSRKQKYA"
     gene            complement(1444..3435)
                     /locus_tag="PLA58_04935"
     CDS             complement(1444..3435)
                     /locus_tag="PLA58_04935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOR62172.1"
                     /translation="MNTDRSTNFFARQARARRNGRRQLIAFAFAVAVIVLVTSLAIRL
                     AWYLYVGTNAYTSFNTGEAYSYQQKLSTFTFFDPAFFIFMSMAIVIVILSASIYKMHT
                     LKKGGAAVAEMLGARRIPPGATDPSERRLINVVEEMAIASGIPVPQVFVLDSENNINA
                     FAAGLELSDAAVTVTRGALAKLNRDELQGVVAHEFSHILNGDMRLNVQLIGILFGILF
                     LGIAGQKFLSGGRVSLRAGLPAMAAGIFLIIIGHTGSFLGRLMQCAIARQQEFLADAS
                     AVQFTRNPSGLAGALKKIGGSAFGSRISSPEARQARHLFFSESHPASWFSFLDTHPPL
                     TTRIRLLDPSFDGKFPKMAEAMREPKPMFTEPFGGTDRYRRPAVAGASALATDVTSWV
                     GNPLPGHLEESRAIVTALPQDIREIAATPRGAASIVFALLVGKDPSRREQQAAALNRV
                     MVLQANPDLVFPLASRLAELSDRLKLPLLELAMPSLSLMTGMEKRNFLLMLHSLIHAD
                     GKRSLLELSVQWILEKHLNPSEDLFRAIRKFSFSQVGLDIVTLLSALASAGHAQDKDK
                     AQKAFDAGVARIPELAARKPVFSFQENTSYETVNRALQSLTDASFKIKESVVDACAHC
                     AFADQTVTFEEGELLRVVALALQCPLPPFIRPSPLPDAA"
     gene            complement(3444..4037)
                     /locus_tag="PLA58_04940"
     CDS             complement(3444..4037)
                     /locus_tag="PLA58_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOR62173.1"
                     /translation="MIPLILLVVILAVVAFYCVGIYNHLITLRNLYRNAFTQIDIQLK
                     RRYDLIPNLVEAAKGYLAHERGTLEAVMKARNSALDASRTAAQSPGSAAAMTGLSQAE
                     GALSGALSRLLAVVEAYPDLKANQAIANLMEELTSTENKISFARQAFNDAVTDYNIQR
                     EKFPQNVIASTFDFKAAALLEVVEKAEERTAPKVSFT"
     gene            complement(4101..5249)
                     /gene="proB"
                     /locus_tag="PLA58_04945"
     CDS             complement(4101..5249)
                     /gene="proB"
                     /locus_tag="PLA58_04945"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOR62174.1"
                     /translation="MNHIRLETLASVKKILIKVGSAVLTGTDGLDLKIIESLVQEMCD
                     LTRRGFSIVLVTSGAIASGKHRLNISGKLKSMPEKQAAAAVGQGRLMRIYSKAFEKNN
                     LYVAQILLTMSDLTDRQRYLNIRNTLSTLMEWHVTPIINENDTVSFDEIKFGDNDNLA
                     AMIANIVEADLFVNLTATDGLFDCNPAQSRKAKLIPLVCEFTDAIEAAATEETSAVGT
                     GGMKSKIAAAKKVTSIGIPCIIAPGKKKKVLSDIMAGREIGTLFLPQTARLASRKYWI
                     AFTLRPRGRLILDDGAKKALLEKGRSLLPSGITAVEGEFDQGDPVACVDREGNLLAKG
                     LVNYSAGDIRRILGLKTSQIYSVLGHKDYDEVIHRDNLVITGEIRKSK"
     gene            complement(5246..6262)
                     /gene="obgE"
                     /locus_tag="PLA58_04950"
     CDS             complement(5246..6262)
                     /gene="obgE"
                     /locus_tag="PLA58_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOR62175.1"
                     /translation="MKFVDEAKIFVRGGHGGAGCVSFRREKFVPKGGPDGGDGGKGGD
                     VIFRAAQTHHTLLDLKYRQHQIAKNGGHGSGNNRTGRSAENLEVVVPPGTLVKDFETG
                     EVLADLAAAGQTFVAARGGIGGKGNAHFKTSTHQTPRFAQEGLEGEERTLLLELKLLA
                     DVGLVGFPNAGKSTFISRVSAARPKIADYPFTTLTPHLGVVKHKDSPAFVVADIPGLI
                     AGASEGLGMGIQFLRHVERTRVLVHLIDISEETHTGAWKNFTAINRELKNYNPQLLDK
                     EQLVCLNKIDLPAVREKVKKTVAQFSKKGIILHPFSAATGEGLQDILGKIVTLLDNTK
                     NLEP"
     gene            complement(6390..6647)
                     /gene="rpmA"
                     /locus_tag="PLA58_04955"
     CDS             complement(6390..6647)
                     /gene="rpmA"
                     /locus_tag="PLA58_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOR62176.1"
                     /translation="MAHKKGQGSSRNGRDSNSQRRGVKVYGGQNIHAGSIIVRQVGTK
                     IHPGNNVGIGKDFTLYSLINGVVKYERLDKTRKKVSVYAAA"
     gene            complement(6660..6971)
                     /gene="rplU"
                     /locus_tag="PLA58_04960"
     CDS             complement(6660..6971)
                     /gene="rplU"
                     /locus_tag="PLA58_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOR62177.1"
                     /translation="MYAIIKTGAKQHKVSEGDVLSVEKLPGEKGAEIVFNEVLMVSDE
                     ADVKIGKPFVEGAKVIGEVVAQTKAPKLIIGKYKKRKGFRKKTGHRQELTSMKIKKIS
                     I"
     gene            7181..8050
                     /locus_tag="PLA58_04965"
     CDS             7181..8050
                     /locus_tag="PLA58_04965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOR62178.1"
                     /translation="MKRRFPCLAFIIFFLVCPPLPAMAGEDPRSFVEGLLVAEGLEPT
                     RASSLLQDTRISICPDIVLKNLFFSEPKGSVQKPDVMDIDPRQIERGRDFMKENAAVL
                     SAVEQRFGASPRIITAILIIESRLGTYPMPYNVVNAYANLAFVLDPAYWKEIQNRYAD
                     AYPQIRDDATSARAKRKAKWAVRELYHLSRIADHLEIDPLSISGSFAGAMGPAQFIPS
                     SFWIFGMDGDGDGLADPSNMTDAKFSMGHYLRKFGWKEETSVEQKRRAIWHYNRSRVY
                     VNTVMMLYEQLGH"
     gene            complement(8073..9701)
                     /locus_tag="PLA58_04970"
     CDS             complement(8073..9701)
                     /locus_tag="PLA58_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOR62179.1"
                     /translation="MDTNKDKNVINDPSGQQKADGGSLRPDLAEAIRRHSFGLDKNRP
                     DAVEKRKKKNQRTARSNIEAFCDPESFVEYGALAIAAQRGRRSVDDLIAKTPADGLIA
                     GIATVNGELYPEDKARCMIMAYDYTVLAGTQGYFNHKKMDRMLKLAHEQKLPLIFFGE
                     GGGGRPGDVDAMSVMVAGLDLGTFGSFARLSGHVPVVGIVSGPCFAGNAALLGCCDVI
                     IAAKNSNIGMGGPVMIEGGGLGVFKPEDIGPMDVQTQNGVVDIEVADDIEAVAVAKKY
                     ISFFQGTLPSWTAGDPLKLRELIPESRKRAYNVRNVIKAIADTDSFVELRPRFGPGMV
                     TGLLRIEGRPFGVIANNSMHMAGAIEAEGADKAARLMMLCNAHGLPILSLCDTPGFMV
                     GPEIEKRAQVRHVCRMFVVGSHVTVPYFTVILRRGYGLGVMAMAKGGFHESFFSVSWP
                     TGEFGGMGLEGAVKAGFKKELAAIEDPQKREEFYESLVAQLYEIGKAINMAAHLEIDA
                     VIDPADTRQWIMRGIKSAAARQPADACHSFVDPW"
     gene            complement(9903..11564)
                     /locus_tag="PLA58_04975"
     CDS             complement(9903..11564)
                     /locus_tag="PLA58_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR62180.1"
                     /translation="MERIWMKSWQQDIPTTLTFEHGKKPIHEYLRIRAGEIPNKTAII
                     FYGREISYRELDEASDRFANYLRQTGVKKGDRVAIFMANCPQYVIAHFGTQKLGAIVC
                     PCSPLFKEMELTYELNDAETEVLVTWDILMPIAGKVLANTKVKKVVITNLNDYLPAQP
                     TLPITDTMKIPRQNIPGTDDFMEIVTKGDKTPPRVAIDLDTDIGLFQYTGGTTGLPKG
                     SMLSFYAALFKTAAVCTITGINKNAVSLVSMPIFHIAGMVAGMNSCIYAGATQVLFTQ
                     FDVKATMEAIQRYKANFWYSAVPMNVGIMMHPDVKNYDLSSMKLCLTSSFGITLTEEI
                     SRQWATFTGGGTLLEGAYGLSETHTADTYVPRDKVKYGTMGIPGFEADFKIVDVNDRS
                     KEMPLGESGEIVLKNPGVFKGYWKKPEETKNTLVDGWVYTGDVGKFDEDGFLYLIGRA
                     KEMIKVSGFSVYPEEVEFMLNKYPGVAQSAVIGIPDPQKMEVVKAFIVMQPGKTATEE
                     EIKIWAKQNMSAYKVPAKIEFRTSLPLLGTGKILRRALKEEPAKT"
     gene            11699..12322
                     /locus_tag="PLA58_04980"
     CDS             11699..12322
                     /locus_tag="PLA58_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR62181.1"
                     /translation="MAVKNGKTANNHLEAESRRRILDAALVLFAQKGYAATTVREIVD
                     AAGITAPSLYYYFGSKEGLYLDLVQTHGAQIDLALQSYAHTSASAKMRVKDLVDKIFL
                     QVINDKDFFRLMFSMYYGPSQGAPYYDFISYHVKIHAAIKKVIEEGIASREFQSGNPG
                     YITWVIRGVVQLAMEEQIKDDREKIDRPRLQRILDIILDRLERPPSP"
     gene            12408..13208
                     /locus_tag="PLA58_04985"
     CDS             12408..13208
                     /locus_tag="PLA58_04985"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR62182.1"
                     /translation="MELMIKDKIAVVTGGGSGIGEAIALRLASEGAKVVVADLDPEAA
                     KKVAGTIQTQGGDAVFVVCDATNPGAVDALMEKTVRTYGAVDILVNNVGGGTGINLVV
                     KTLLEDWDKTIELNLRSAYLCCCAAAKIMIPRKQGRIINMSSISGKQGEPLLGAYCAA
                     KSGVIGFTQTLARELARYDITVNAVCPGYVFTPGWEKLAQALKDSQPALADKSLLEIF
                     EARVRSVTPLRRPQEASEIAGLVAYLVSYEARSITGQAISIDGGIATG"
     gene            13516..14751
                     /locus_tag="PLA58_04990"
     CDS             13516..14751
                     /locus_tag="PLA58_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR62183.1"
                     /translation="MKYLKGLVCLFVAVFVMSWTGSSRADEIVLGYTGPLSGPAAEYG
                     QDILYGVEMAVNEINAAGGIFVSGKKYTFKLIKLDDRADPTQAVNNARRFQSDKAIAV
                     FNGVFNTTAPMMKINEEKGREFIMMAYTSTPKIETLGNKLMVDTTVTFTTYVQVFAEW
                     AFKKGWKKCAMVVTLGAYGDEWRQSFREYWEKRGGVITADKPANYYTETDFSSPIAAA
                     LATKPDVMLIGGPSATTALVIEQARGMGFKGGFIMIDQAKQDYIAKVLKSVKVMDNLI
                     GTGGVVSVAMPGSVFEKRYTEKYKRMVTWECALNYTGVYALAKAMTVAGTVSDAYRIR
                     ASFAKALETPMLGDKYPNEVFGIEPAGRLRIMASVGTITNGKFDKSELYVWWGDQREY
                     ERVKKLSTATSNPIIWLKR"
     gene            15082..16329
                     /locus_tag="PLA58_04995"
     CDS             15082..16329
                     /locus_tag="PLA58_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR62184.1"
                     /translation="MKYLKGLVCLFVAVFVMSWTGSSRADEIVLGYTGPLSGPAAEYG
                     QDILNGVDMAVKELNAGGGISVKGKKYTFKLERLDDRIDPTQAVNNARRFKSNGAIAV
                     FNGVFNTIAPIMKINEEKGAEFIMMAYTSTPLAEKLGNNLLVIATPDFNDYVRVFADW
                     ALKKNWKKCAMVVTFGSYGDEWRSAFKEYWTKKGGVITADKPANYYTETDFSSPITAA
                     MATKPDVMLIGGPSATTALVIEQSRNMGFKGGFIMIDQAKQDNIAMLLRSTKIMGDLI
                     GTAGVLSIPFDGARAFDKKYTSTYKRMVTWECALNYAGMHALARAIATAGTVDDVYKI
                     RAAFSKALDPPMLGDKFPTSVFGISSAGRLHIMSSVQTITKGKSDPSVLYAWWPTTQK
                     EFDKIAVQSAVGGGTNPVYWLQR"
     gene            complement(16454..17419)
                     /locus_tag="PLA58_05000"
     CDS             complement(16454..17419)
                     /locus_tag="PLA58_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62185.1"
                     /translation="MKMKEIKASAMNVEKFIDDKVRDIRNAVGNGLAVNALSGGVDSC
                     VVTALGHKALGNRLKTYMIDNGIMREGEPARVQAAFRKLGITVEVIDASKAFFAALRG
                     LTDPEEKREAITQTFYKNVFGKLVVKSKAQYLLQGTILTDVDETVAGIKRQHNVFEQL
                     GIDPQKAFGYRILEPLIELRKDGVRKAGAGLGLPSAMFDRIPFPGPALAARVIGEVTP
                     AKIALVRKATAITEAALKDTKAFQYLAILHEDRVTGMRDGKRVFGNQIEIRCWESTDA
                     RKARPSRLPFDVLEKIAAKITKEIPEVVSVTYNITTKPTSTIEAT"
     gene            17626..18387
                     /gene="hisF"
                     /locus_tag="PLA58_05005"
     CDS             17626..18387
                     /gene="hisF"
                     /locus_tag="PLA58_05005"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOR62186.1"
                     /translation="MEPIKIMPCLDMKNGRVVKGVHFVDIREAGDPVENAVFYQAEGA
                     DELAMLDIAATLENRKTRLEWVKKVSAQISIPLTVGGGISSLEDIEMVLGAGASKVSM
                     NSAAVKNPELVRLASAKFGPEKITVAIDAKRNPAMSSGFELVVSGGTLPIAKDAVAWA
                     RQCQELGAGVILPTSMDGDGTKAGYDIPFTKAISSAVSLPVVASGGAGKLEDFYEAVV
                     DGGASVLLAASVFHFRLLKIQDVKDFLKSKGLAVA"
     gene            18425..19072
                     /locus_tag="PLA58_05010"
     CDS             18425..19072
                     /locus_tag="PLA58_05010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR62187.1"
                     /translation="MIKWADIIFFRKHGVCPRWFCFTFDNGLRRLVQNPDRIIRPYVG
                     EGDKVLDVGPGIGYFTIPMARRVGSGGRVIAADIQESMLRGIVRRAERAGVRGQIELY
                     LSRPDEIYSGEPVDFILSFWMAHEVPRLDRFFRQLRAALKPAARFLLAEPKLHVSRTQ
                     FDGEIAAAQKAGLALLERPAISLSHGALFGSGRVGTSGRFPADQREASPVSRKEH"
     gene            19081..19512
                     /locus_tag="PLA58_05015"
     CDS             19081..19512
                     /locus_tag="PLA58_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOR62188.1"
                     /translation="MKNRVWTGIIFLILMPLWAWAAEYDTGVQSRVILQAETMSNGEP
                     IDYLDADRPQVTVMTVDIAPGASTGWHSHPMPVYAYVMAGQLTVEIEGGKTAEFKEGD
                     AIIEVVKLKHNGTNHGKVPVKLVVFYLGAKDVSNVIRADRP"
     gene            complement(19528..19947)
                     /locus_tag="PLA58_05020"
     CDS             complement(19528..19947)
                     /locus_tag="PLA58_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOR62189.1"
                     /translation="MKSFREELWFDIPRRRAFVNITGNVEAVLKKSGVQEGLALVNAM
                     HITASVFINDDEPGLHADYEDWLERLAPHAPTSFYRHNRTGEDNGDAHLKRQVMGREV
                     VVAVTKGKLDFGPWEQIFYGEFDGGRKKRVLVKIIGE"
BASE COUNT         4261 a         5232 c         5899 g         4657 t
ORIGIN      
        1 atgagattgc ttcacttcgt tcgcaatgac taagagacat catgcaaagg cttcgcattc
       61 tccggttgct cttaggcgta tttttgcttt ctgctgcctt catagagatc atattccagc
      121 agccggcact cgatttcccc gttgtagaag gtcatccggc gcttcgtgcg cagtcccact
      181 ttcttgatca tttccagatt tccggtgaag aggtatccgt ggtagccgcg gccgatgcct
      241 ttcagccaat cgccgcagcc ttcatagagc gcttccagtc gttgaagggc gctgggactg
      301 ctgcgcttat gagcgagatc ggcagctttg cggacgatga ctttttgccc cggcgcgatt
      361 tccttttttg gcgcggattg cgccgttttg atctttagag gctccagccg ttcgccgtag
      421 ggcggattga tgatgatgac gccctttcct cccggggtgg gcgttttttc gaaggggcag
      481 accttgaatt caatcaggcg gtcaacgccg gccgtctgtg cgttgcggcg cgcggcctcc
      541 accgcttgcg gatcgatatc cgaagcgata acggttgagg gcagcgcttt taaagaatca
      601 ttgcgggctt tcctgcgcag ctcctgccag gagtcatggg ggaaatccct gatatgcatg
      661 aagccgtaat tgccgcggcc caggcccggc gcgcggtgaa gcgccatcag ggccgcctcg
      721 atcgcgaggg tgccgctgcc gcacatcggg ttgacgagcg gcgtccggcc ctgccatccc
      781 gtggctttga tgagggcggc cgccagcgtt tcctgcatcg gcgccgccag cggaatcttg
      841 cggtagccgc gcagcgaaag cctttccccg gacgtgtcga tatacgccat ggcctggtcg
      901 ttgcgccagt aaacatgaat gaccgcccgg tttctttccg gcccggaatc cggccggacg
      961 cctgtcttat cgcggatgcg atcgacgatg gcgtccttgg ccctgaggtt ggcaaagcgt
     1021 gtgtcggtga tctccgggtg atcgaccacg gaggtgaccg agaggtaagc actgggaccg
     1081 gaatcgtgca gaaacgtttc ccagggaatg gcgttcagac gctcgtaaaa agcgccggcg
     1141 gagatggcct tgaagatttg cagttgatac aggacgtgct gggccgtgcg cagatgcagg
     1201 ttcaacacca tcgtgtccga cagggttccc tcggtggaaa cggccgtgtc gatttcctgg
     1261 aggatgggga accccagctc ctcgatttcc gccttcagat agggaacgac ccctttcggg
     1321 cacgtgatca atatcctgtt tttcttctgc cagatggaca tgggcaggct ttaacacagg
     1381 ccatggggct tggcaatctt taaaaaaatt cagatttttt aaaacctgcg gggatgggtt
     1441 ggctcaggcg gcatccggca ggggcgatgg ccggatgaac ggcggcaggg ggcactgcag
     1501 cgccagcgcg acaacgcgca ggagttcacc ctcctcgaac gtgacggttt gatccgcgaa
     1561 ggcgcagtgc gcgcaggcat cgacgacgga ttccttgatt ttgaaggaag catcggtcag
     1621 cgactggagc gcccggttca cggtttcgta ggaggtgttt tcctgaaacg aaaagacagg
     1681 tttccgggcg gccagctccg ggatccttgc cacgcctgcg tcaaatgcct tttgcgcttt
     1741 atctttgtct tgcgcgtgcc cggctgaggc cagagcggaa agaagggtca caatgtccag
     1801 ccccacctgg gagaaggaaa atttccggat ggcgcgaaac aaatcttcgg acggatttaa
     1861 atgcttttcc agaatccatt gaacggacag ctccagcagg ctccgtttgc cgtcggcatg
     1921 gatcagggaa tgcagcatca gcagaaaatt tcgtttttcc atcccggtca tgagcgacag
     1981 agacggcatg gccagctcca gaaggggcag tttcagacgg tcggataatt cggcgagccg
     2041 cgaggccaga gggaaaacca gatcgggatt tgcctgcagg accatcaccc gattcagtgc
     2101 cgcggcctgc tgttcgcggc gcgaaggatc cttgccgacg aggagggcga agacaatgga
     2161 agcggcgccc cgcggcgttg cggcgatttc gcggatgtcc tgcggcaggg cggtcacaat
     2221 ggcccggctt tcttcgagat ggccgggcaa aggattgccg acccaagagg taacgtccgt
     2281 cgccagcgcc gacgcgccgg caacggcagg ccggcgatac cggtcggttc ccccgaacgg
     2341 ttccgtgaac atgggtttgg gctcgcgcat tgcttcggcc attttcggaa acttgccgtc
     2401 aaaggacgga tccagcagac ggatccgggt ggtaagcggc ggatgcgtgt ccagaaagga
     2461 aaaccagctt gcggggtggc tttcgctgaa aaacagatga cgggcctgtc gggcctccgg
     2521 cgaggagatg cgggaaccga aggcggagcc gccgatcttc ttgagcgcac ccgccagccc
     2581 cgaagggtta cgcgtgaact gcacggcgga ggcgtccgcc agaaactcct gctgacgggc
     2641 gatcgcgcac tgcatcaggc ggccgagaaa cgagccggtg tggccgatga tgatcaggaa
     2701 gattcccgcg gccatggccg ggagccccgc gcgcagcgat acccgaccgc ccgagagaaa
     2761 tttttgtccg gcaatgccca ggaaaagaat gccgaagagg atgccgatca gctggacgtt
     2821 gaggcgcatg tcgccgttga gaatatggct gaactcatgg gccacgacgc cctgcagttc
     2881 gtcgcggttg agtttggcga gcgctccccg cgtgacggtc accgccgcgt cgctcaattc
     2941 cagtccggcg gcaaaggcgt tgatgttgtt ttcggaatcc agaacaaaga cctgcggaac
     3001 gggaatgccc gaggcaatgg ccatttcctc cacaacgttg atcagccgcc gttcgctcgg
     3061 atcggtcgcg ccggggggga tgcgccgggc gcccagcatt tccgccacgg cggcgccgcc
     3121 ttttttcagc gtgtgcattt tgtagatgct ggcggagagg atcacgatga cgatggccat
     3181 ggacatgaag atgaaaaacg cggggtcgaa aaacgtgaag gtggaaagct tctgctggta
     3241 gctgtaagct tctccggtgt tgaaggaggt gtaggcgttt gtgccgacat acaggtacca
     3301 ggccagacga atggccaggg aggttaccag cacaatcacg gccacggcga aggcaaacgc
     3361 gatgagctgc cgccggccgt tcctgcgggc ccgcgcctgc ctggcaaaga aattggtcga
     3421 acggtcggta ttcatgcttc ccgtcaggtg aaggacactt tgggcgctgt gcgctcttcg
     3481 gctttttcca cgacttccag gagggcggcg gccttgaaat caaaggtgga cgcgatcacg
     3541 ttctggggga atttttcccg ctgaatgttg tagtcggtga cggcgtcgtt gaaagcctgg
     3601 cgggcgaagc tgatcttgtt ttccgtggag gtcagctcct ccatcagatt ggcgatggct
     3661 tggttggctt tcaaatccgg gtaggcttcg acgaccgcca gcagtctgga aagggctccg
     3721 gaaagcgctc cttccgcctg gcttaggccc gtcatggccg cggcgctgcc gggagactgg
     3781 gcggcggtgc ggcttgcgtc cagggcggaa ttgcgcgcct tcatgacggc ttcgagcgtg
     3841 ccgcgttcgt gcgccaggta gcctttggcg gcttccacca ggttgggaat cagatcatag
     3901 cggcgcttga gctggatgtc gatctgggtg aaagcgttcc ggtatagatt gcgcagagtg
     3961 atcagatgat tgtaaattcc gacgcaataa aaggcgacga cggcgaggat gacgaccaga
     4021 aggatcaatg gaatcatgtt gctctccttg caatggcgcc cggacgggcg cagggataat
     4081 gctcccggat gatcggacca tcacttgctt ttcctgatct cgccggtgat gaccagattg
     4141 tcgcgatgaa tcacttcatc atagtctttg tgccccagca cggagtaaat ctgcgatgtc
     4201 ttcaggccga ggatgcggcg gatgtctccg gcgctgtagt tgaccagccc cttggccagc
     4261 aggtttcctt cgcggtccac gcaggcgacg ggatcgcctt gatcgaattc cccttcgacg
     4321 gcggtgatgc ccgagggcag caggctcctg cctttttcga gcagggcttt cttggccccg
     4381 tcgtcgagga tcaggcgtcc gcgggggcgc agcgtaaaag caatccagta cttgcggctg
     4441 gccagtctcg cggtctgcgg cagaaacagc gtgccgattt ccctgcccgc catgatgtcg
     4501 gacagcactt tctttttttt gcccggcgcg atgatgcagg gaatgccgat ggaggtgact
     4561 ttcttggcgg cggcgatttt gcttttcatg ccgcccgtgc cgacggcgga ggtttcctcc
     4621 gtggcggctg cttcgatggc gtcggtgaat tcgcacacca ggggaatcag cttcgctttt
     4681 ctggattggg cgggattgca gtcgaaaagc ccgtcggtcg ccgtcagatt gacgaacagg
     4741 tcagcctcga cgatattggc aatcatggcg gcaaggttgt cgttgtcgcc gaatttgatt
     4801 tcatcgaagg agacggtgtc gttttcgttg atgatcggcg tgacgtgcca ttccatcagc
     4861 gtggaaagcg tgttgcggat gttgagatag cgctgccggt cggtcagatc cgacatggtg
     4921 agcagaatct gcgcgacgta gaggttgttt ttctcaaacg ccttggaata gatgcgcatg
     4981 agccggccct ggccgaccgc cgccgccgcc tgtttttccg gcatgctttt gagtttgccc
     5041 gagatgttca ggcggtgctt gcccgaggcg atggccccgg acgtgaccag gacgatggaa
     5101 aatccccggc gcgtcaggtc gcacatttcc tggacgaggg attcgatgat tttcaaatcc
     5161 agcccgtccg ttccggtgag caccgcagag ccgaccttga tgagaatttt cttgacggat
     5221 gcgagcgttt ccagacggat atgattcatg gctcaaggtt tttcgtgttg tctaagaggg
     5281 ttacgatctt tcctaaaata tcctgcaatc cttctccggt cgcggcggaa aacggatgca
     5341 gtattatccc ttttttcgaa aattgcgcaa cggttttttt gaccttttcc cgtacggcgg
     5401 gcaggtcgat tttgttgagg cagacgagtt gctctttgtc caaaagctgt ggattgtagt
     5461 ttttcagctc ccggttgatg gcggtaaagt ttttccaggc gccggtgtgc gtttcttcgg
     5521 agatgtcgat cagatgaacg agcacacggg tgcgctccac atggcgcaga aactggatgc
     5581 ccatgccgag gccctccgaa gccccggcga tcagacccgg gatgtcggcg acgacaaaag
     5641 ccgggctgtc cttgtgcttc accactccca gatgcggtgt aagcgtggtg aagggatagt
     5701 cggcgatttt gggccgggcg gccgatacac gggaaatgaa ggtggatttg cccgcattgg
     5761 ggaatcccac cagcccgacg tccgccagca gcttcagttc cagaagcagc gtgcgctcct
     5821 ctccctccag gccttcctgg gcgaaacgcg gcgtttggtg ggtggaggtc ttgaaatggg
     5881 cgttgccctt gcctccgatg ccgccgcggg ccgcgacgaa ggtctgtccc gccgcggcca
     5941 gatctgcgag aacctcgccg gtttcaaagt ccttcaccag cgttccgggc ggtacgacaa
     6001 cttcaagatt ctccgcgctg cggccggtgc ggttgttgcc cgagccgtgc ccgccgtttt
     6061 tagcgatttg atgctgacga tatttcaaat ccagcagggt gtggtgcgtt tgggccgccc
     6121 ggaagatgac gtcgccgccc ttgccgccgt cgccgccgtc cggaccgcct ttggggacga
     6181 acttctccct ccggaaactc acgcagcccg cgccgccgtg tccgccccgg acgaatatct
     6241 tggcttcgtc aacaaatttc acttgtttct ttcctctttt gaagaaggga acggtctcgc
     6301 cgcccagagc gttgtcgcga ccttttcctc aaagaaaaaa ggcgcttaat gtctcaagcg
     6361 ccttttgaac acgagttgga atgaccggtt tacgccgccg catagacgct gacctttttg
     6421 cgggtcttgt ccagcctttc atatttgacc acgccgttga tgagagaata caacgtgaaa
     6481 tccttgccga tgcctacatt gttgccgggg tgaatttttg ttccgacctg ccgcacgata
     6541 atggaacccg cgtggatatt ctggccgccg tacactttga cgccgcgccg ctgcgaattg
     6601 gagtctctac cgttgcggga gcttccctga ccttttttat gtgccatgat atcctccgct
     6661 tatatcgaaa tcttcttgat tttcatgctg gttaattcct gccgatggcc ggtttttttg
     6721 cgaaatccct tgcgcttttt gtatttgccg atgatcagct tcggggcttt agtctgggcg
     6781 accacttcgc cgatgacttt ggcgccctca acgaaaggct tgccgatttt cacatcggcc
     6841 tcgtccgata ccatcaaaac ttcattaaaa acaatttcgg cccctttttc accgggcagt
     6901 ttttcaaccg ataatacatc gccctcgctg actttgtgct gtttggctcc tgtttttatg
     6961 attgcgtaca taaccttatc cttcgttagc gttaagattg ggacttatag acaaaatgct
     7021 cctttttgtc aatagaattc agccaccctg atgatatttt ataaagcaag ccaccggcaa
     7081 gtctaaaata ttgatatcaa gaagaaattc gcttgcattc caccgcggcg aaaatgtagg
     7141 atagctcatc aatggcatga aagaattccg gggtggatcc gtgaaaagac gtttcccttg
     7201 tcttgctttt attatttttt ttctggtctg tccgccgctc ccggcaatgg ccggcgagga
     7261 tccgagatcg tttgtcgagg gattgctggt tgccgagggg cttgaaccaa ccagagcaag
     7321 cagccttctg caggacaccc gcatatccat ctgcccggat atcgttctga aaaacctatt
     7381 cttttccgaa cccaaaggtt ccgtgcagaa gcctgacgtg atggatattg atccccgtca
     7441 gattgaacgg ggcagggatt ttatgaaaga aaacgcagct gttttgtccg ccgtggaaca
     7501 gcgcttcggc gcgtcgccga ggatcatcac cgcgattctc atcatcgagt cacggctggg
     7561 gacctatccc atgccgtata atgtggtcaa cgcttacgcc aacctggcct ttgtgctgga
     7621 tccggcctac tggaaagaaa tccagaaccg ctatgcggac gcctatccgc agattcggga
     7681 tgacgcgacg tccgcccggg cgaaaagaaa agcgaagtgg gcggtgcggg agctttatca
     7741 tctgtcccgg atcgccgatc acctggagat cgatccgttg agcatttccg ggtcttttgc
     7801 cggcgcgatg ggcccggcgc agtttattcc ctcgtctttc tggattttcg gaatggacgg
     7861 cgacggagac ggcctggctg atccctcgaa catgacggat gccaaattca gcatggggca
     7921 ttatctcaga aaattcggct ggaaagagga aacttccgtt gaacaaaaac gcagggcgat
     7981 ctggcactac aatcgcagcc gcgtgtatgt aaatacggtg atgatgctct acgaacaact
     8041 gggacactga aagacgcccc ggttgcctgt tcctaccacg gatcgacaaa agaatgacag
     8101 gcatccgccg gttgccgggc agccgcggat ttgatccccc gcatgatcca ctggcgggtg
     8161 tccgccggat cgatgacggc gtcaatttcg aggtgcgcgg ccatgttgat ggctttgccg
     8221 atttcgtaga gctgggccac gagtgattcg tagaattctt cacgcttctg cggatcctca
     8281 atggccgcca gttctttttt gaaaccggct ttaacggcgc cttccaggcc cattccgccg
     8341 aattcccccg tcggccagga cacgctgaag aaagattcat ggaatccgcc tttggccatc
     8401 gccatgacgc cgaggccgta gccccggcgc aggatcaccg taaagtaagg aacggtgaca
     8461 tgcgagccga cgacgaacat ccggcagaca tggcggacct gcgcccgttt ttcaatttcg
     8521 gggccgacca tgaagcccgg cgtgtcgcac agcgacagaa tcggcagtcc gtgcgcgttg
     8581 cacagcatca tgaggcgcgc ggccttgtcc gcgccttcgg cttcaatggc gccggccatg
     8641 tgcatggaat tattggcgat tacgccgaac ggacgccctt cgatgcgaag cagccccgta
     8701 accatgcccg ggccgaaccg ggggcgcagt tcgacgaagg aatccgtatc cgcgatggct
     8761 ttgatgacgt tgcgcacatt gtaggcgcgc ttgcggcttt ccggaatcag ctcgcgaagt
     8821 ttgagcggat cgcccgccgt ccacgagggg agagtgccct gaaaaaagga aatatatttt
     8881 ttggccacgg cgacggcttc gatgtcgtcg gcgacttcaa tatccaccac gccgttctgc
     8941 gtctgaacgt ccatcggccc gatatcctcg ggtttgaaaa cccccagccc gccgccttcg
     9001 atcatcaccg gaccgcccat gccgatgttg gaatttttcg cggcgataat gacgtcacag
     9061 cagcccagaa gcgcggcgtt tcccgcgaag caggggccgg aaacaatgcc gacgaccgga
     9121 acatgcccgc tgagtctggc gaaagaaccg aacgtgccca gatccagtcc cgcgaccatc
     9181 acgctcatcg cgtccacgtc gccgggtctt cctcctccgc cttcgccgaa aaagatcagc
     9241 ggcagcttct gctcgtgggc cagtttcagc atgcggtcca ttttcttgtg gttgaaatat
     9301 ccctgcgtgc cggccagcac agtgtagtcg taagccatga tcatgcagcg ggctttgtct
     9361 tcgggataaa gttcgccgtt taccgtggcg attccggcga tcaaaccgtc ggccggcgtt
     9421 ttggcgatca ggtcgtcaac ggaacggcgt ccccgctgcg cggcaatggc cagcgctccg
     9481 tattcaacga aactttccgg atcgcagaag gcctcgatgt tggagcgggc ggtgcgctga
     9541 ttcttctttt ttcttttctc cactgcgtcg gggcggtttt tatccagtcc gaaggaatgt
     9601 ctgcggatcg cttcggccag gtcgggacga agggatccgc cgtccgcttt ttgttgcccg
     9661 gagggatcat tgatgacgtt tttgtcttta tttgtatcca tcttctttca actccctgtg
     9721 tttattgaga tgcatgatcg tgttcatttc aggcgggcga cgcccgcgaa aaagcgtctg
     9781 ccggtctgcg aggatgccat attcattgat tttttatttc cactttttta tgagaattcc
     9841 gcccggggcg gaaccggaag atagcgttcg gcgctttgcc ccgggtggaa ggaaacgtgt
     9901 cgttaggttt tcgccggttc ttccttcagt gcccggcgca agatttttcc ggtgcctaaa
     9961 agaggcagag atgtccgaaa ctcgattttg gcgggcactt tgtatgcgga catgttctgc
    10021 ttggcccaga ttttgatttc ttcttccgtg gccgtcttgc ccggctgcat cacgatgaag
    10081 gcttttacga cttccatctt ctgcggatcg gggatgccga tgacggccga ctgggcgacc
    10141 cccgggtatt tgttgagcat gaattcgacc tcttcgggat aaacggagaa gccggaaact
    10201 ttgatcatct ccttcgcgcg tccgatcaga tatagaaatc cgtcttcgtc gaactttccc
    10261 acatcgccgg tataaaccca cccgtcaacg agcgtgtttt ttgtctcttc cggttttttc
    10321 cagtagcctt tgaaaacgcc gggatttttc aaaacgattt ctccggattc tcccagaggc
    10381 atttccttcg accggtcatt gacgtccacg attttaaagt cggcttcgaa gccgggaatg
    10441 cccatggtgc cgtactttac cttgtcgcga gggacatacg tgtcggcggt gtgggtttcg
    10501 ctcaatccgt atgctccttc cagtagcgtg cctccgcccg tgaaggttgc ccactgacgg
    10561 gatatttctt ccgtcagcgt aatgccgaaa gaggaggtga ggcagagttt catgctggaa
    10621 aggtcgtagt ttttaacgtc cggatgcatc atgatgccga cattcatcgg cacggcgctg
    10681 taccagaaat tggccttgta gcgctggatg gcttccatcg tggccttgac atcgaactgc
    10741 gtgaagagga cctgcgtggc gccggcgtaa atgcaactgt tcatgccggc gaccatgccg
    10801 gcaatgtgga aaataggcat ggaaacaagg ctgacggcat tcttattgat tccggtgatt
    10861 gtgcagaccg ccgctgtttt gaatagcgcg gcgtaaaagc tgagcatcga tcctttgggc
    10921 agaccggttg tgccgcctgt atactggaag aggccgatgt cagtgtccag atcgatagcg
    10981 acgcgggggg gtgttttgtc gcctttggtg acaatttcca tgaaatcatc cgttcccgga
    11041 atattttgcc gggggatttt catcgtgtcg gtaattggca gggtgggttg cgccggcaga
    11101 tagtcgttga gattagtgat cactactttt ttcactttgg tgttcgcgag cactttcccg
    11161 gcgatcggca tgaggatgtc ccaggttacg agcacttctg tttccgcgtc attgagttca
    11221 taggtcagtt ccatttcctt gaagagggga gagcaggggc agacaatggc gcccagtttc
    11281 tgtgtgccga aatgcgcgat gacgtactgg gggcaattgg ccatgaaaat ggcgacccgg
    11341 tcgccctttt tgacgccggt ttgccggaga taattggcaa accggtctga cgcttcatcg
    11401 agttcgcgat agctgatttc ccgtccgtaa aaaatgatgg cggttttatt tgggatttct
    11461 ccagcccgga ttctcaagta ttcgtggata ggttttttgc cgtgttcaaa ggttaacgtt
    11521 gtgggaatgt cttgctgcca gcttttcatc caaatgcgtt ccatacaaaa acctcccaaa
    11581 tttttattta tcttatgatc attgacaaat ggggatactt gccaatatag taagcgggaa
    11641 ttgcttctgt agcgagcgtt agatagaata catcaagcct aaagtcaagg ataaaaaaat
    11701 ggctgtaaaa aacggaaaga cagcaaacaa ccatctggaa gcggaatccc gccggcgaat
    11761 cctcgacgcc gcgctcgtat tgtttgccca aaagggatac gccgccacca cggtgcggga
    11821 aatcgtggat gcggcgggaa tcaccgcgcc ttccctgtat tattattttg gaagcaagga
    11881 ggggctttat ctggatctgg tgcaaacgca cggagcacaa attgatctgg ctttgcaatc
    11941 ttacgcgcat acatccgcaa gcgcgaaaat gcgggtgaaa gatcttgtgg acaagatttt
    12001 tcttcaggtg attaatgaca aggatttttt ccggttgatg ttcagcatgt actatgggcc
    12061 gtctcaaggc gcgccttact atgattttat ttcttatcat gtcaaaattc atgcggcgat
    12121 aaaaaaggtc atcgaagaag gcattgcctc aagggaattt caatcgggca atccgggata
    12181 catcacctgg gtgatccgcg gcgtggttca gctggcgatg gaggagcaaa tcaaggatga
    12241 cagggaaaaa atcgacaggc cccgcctgca aaggattctg gatatcatcc tggaccgtct
    12301 ggaacggccg ccttcccctt aaaaaaactt gatggccggc agagggacca tggccatcct
    12361 gcatcgattg aaggatcagt aaagatcatt ccgagggggg aatatttatg gaactgatga
    12421 tcaaagataa aatcgctgtt gtgacgggcg gcggaagcgg aatcggcgag gccatcgctt
    12481 tgcgtctggc ttccgaaggc gcgaaagtgg ttgtggcgga tctggatccg gaggcggcaa
    12541 aaaaagttgc cggaaccatt cagacgcagg gaggggatgc cgtttttgtt gtctgcgatg
    12601 ccacgaatcc gggagcggtc gacgcgctca tggagaaaac cgttcggact tacggcgccg
    12661 ttgatatact ggttaacaac gtgggcgggg gcacgggcat caacctggtg gtgaaaacgc
    12721 tccttgagga ctgggacaaa accatcgagt tgaatttaag aagcgcttat ctttgttgtt
    12781 gcgcagcggc taagatcatg attccaagga aacagggacg catcatcaat atgtcctcca
    12841 tttcgggaaa acagggagaa ccgcttctgg gtgcatactg tgccgccaaa tccggagtca
    12901 tcggatttac acaaaccctg gcccgggagc tggcgcgcta cgatatcacg gtcaacgccg
    12961 tctgccccgg ctatgttttc acgccgggct gggagaaact ggcgcaggcc ctcaaggatt
    13021 cgcagccggc cctggcggac aaaagcctgc tggaaatctt tgaggcgcgc gtccgatccg
    13081 taacccccct gcgccgtcct caggaagcct ctgaaatcgc agggctggtg gcctatctgg
    13141 tgtcttatga agcccgcagc attaccggtc aggcgatcag catcgacggc ggcatcgcga
    13201 cgggttaggg gatgcgtccg ttcatttgtt gccgccggca catgaagaat tattccacct
    13261 gaaaaaactg accggcagat attttacgaa tggcggtcat ctcatttctt taatgatatt
    13321 ggaatattat ttaaaaagaa ctcggtcatt ttacacgatg cttcgagcgc aatcgattta
    13381 tggtattgac aagctattcg caaatcatta gacaatttat catacagtaa aatcagatag
    13441 ggagaagggg aagacggact catcattttc ccgatgtcgt atggtcaaga aattcgccaa
    13501 cgaggaggaa ctgaaatgaa gtatctgaag gggttagttt gtctgtttgt tgcagttttt
    13561 gtcatgtcct ggacgggaag cagcagggct gatgaaattg ttctcgggta cacaggaccg
    13621 ctttccggac cggcggcgga atacgggcag gacattctct atggcgtgga gatggcggtc
    13681 aatgaaatca acgcagccgg cggaattttt gtaagcggca agaagtacac gttcaagctg
    13741 ataaagctcg acgaccgggc ggatccgacg caggccgtca ataatgcgcg ccgttttcaa
    13801 tcagataaag ccatcgccgt ttttaacggt gtgttcaaca ccacagcgcc catgatgaaa
    13861 atcaatgaag aaaaaggaag ggaattcatc atgatggcct ataccagcac gcccaagatc
    13921 gaaacgctgg gcaataagtt aatggtggac accacagtaa cctttacgac ctatgtccag
    13981 gtctttgcgg agtgggcttt caaaaaaggc tggaagaaat gcgccatggt cgtaacgctg
    14041 ggagcctacg gcgatgaatg gcggcaaagc ttcagagaat actgggaaaa aagaggtggt
    14101 gtcattactg cggataaacc tgccaattac tatacggaaa ccgatttttc ctcgccgata
    14161 gccgccgcgc tggcgaccaa gccggacgtt atgctgatcg gcggaccttc cgcgacgacg
    14221 gccttggtga ttgaacaggc cagaggcatg ggtttcaagg gtggcttcat catgattgat
    14281 caggccaagc aggattacat cgccaaagtg ctcaagagcg tcaaagtgat ggacaatctg
    14341 atcggcaccg gcggcgtggt cagtgtggcg atgccgggct ccgtctttga gaaacgctat
    14401 acggagaagt acaaaagaat ggtaacctgg gaatgcgctt tgaactacac cggcgtttac
    14461 gccctggcca aagcgatgac ggttgcgggc acggtgtccg acgcatacag aatccgtgcg
    14521 tcgtttgcca aagcgctcga gacacccatg ctgggtgaca aataccccaa cgaggttttc
    14581 ggtattgaac ctgccggacg tctgcgaatt atggcctccg tcggtaccat tacaaacggc
    14641 aagtttgaca agtcggagct ttatgtgtgg tggggagacc agagagaata cgagcgagtc
    14701 aaaaaactgt ccacggcaac cagcaatccc attatctggc tgaaacgata gggtgaaaat
    14761 tttccggatc aaggggggca gggaaacctg cctctcctct ttttttgcac ccggcgatcc
    14821 aactgaaaaa accgaccggc agatattatg agaatggcgg ttatcgtttt tattcaataa
    14881 tatcctatgc atactgaaga aaaccagggt cgtttcgcgc ggtgattccc ttgcaagcga
    14941 tttatgtcat tgacaaacgc tctgcaactc attaagaaaa catccatact tgtaaagtca
    15001 aataggggag aaggggaaga cggactcatc attttcccga tgtcgtatgg tcaagaaatt
    15061 cgccaacgag gaggaactga aatgaagtat ctgaaggggt tagtttgtct gtttgttgca
    15121 gtttttgtca tgtcctggac gggaagcagc agggctgatg aaattgttct cgggtacaca
    15181 ggaccgcttt ccggaccggc ggcggaatac gggcaggaca ttttaaacgg cgttgacatg
    15241 gccgtgaaag agctcaacgc ggggggaggg attagcgtca aagggaaaaa atatacgttc
    15301 aaactggagc ggctggacga ccggatcgat ccgacgcaag cggtgaacaa cgcccgccgg
    15361 tttaaatcca acggagccat tgccgtattc aacggcgttt tcaatacgat tgctccgatc
    15421 atgaaaatca atgaagagaa gggcgccgag ttcatcatga tggcttacac cagcacaccg
    15481 ttggctgaaa agctggggaa caatctgctg gtaattgcca cgcctgattt taacgactat
    15541 gttcgtgtat ttgccgactg ggcattgaaa aaaaattgga aaaaatgtgc aatggttgtc
    15601 acgtttggtt cgtacggaga tgaatggaga agcgcattta aagagtattg gaccaagaag
    15661 ggcggcgtga ttacggcgga caagcctgcc aattattata cggaaacgga tttttcctcg
    15721 ccgatcaccg ccgcgatggc gaccaagccg gacgtcatgc tgatcggcgg accttccgcc
    15781 acgacggctc tggtcatcga acagtccaga aacatgggct tcaagggcgg atttattatg
    15841 atcgatcagg ccaagcagga taacatcgcc atgctgctcc gaagcactaa aatcatgggc
    15901 gacttgatcg gaacagcggg agtgttgagc attccttttg acggcgccag agccttcgat
    15961 aaaaaatata cttccaccta caagcgcatg gtgacctggg aatgcgccct caattacgca
    16021 gggatgcacg ccctggcccg cgcgatcgct accgctggga ccgttgacga tgtgtataaa
    16081 attcgggctg ctttttccaa ggccctggat ccgccgatgc tgggagataa atttccgaca
    16141 tcagtattcg gcatcagcag cgcggggcgt ctgcacatca tgagttccgt tcaaacgatt
    16201 acaaagggca agtccgatcc atccgttctt tatgcctggt ggccgacaac tcaaaaagaa
    16261 tttgacaaga tcgcggttca gagcgccgtg ggcggtggga cgaacccggt ttactggctg
    16321 caacgctaaa cgcgaaaagc aatccggatc agggagagga ggcggggaaa cctgccccct
    16381 ttttttttga agtttgcgcc aaacgatccg ctgccggttc cggcgggaag ctatcccgcc
    16441 ggaaccctga tcatcaagtc gcttcgatgg tggaggtcgg cttggttgtg atgttgtagg
    16501 tgacgctcac gacttccgga atctctttgg tgatttttgc ggcgattttt tccagcacgt
    16561 cgaaaggcag ccgggagggc cttgccttgc gcgcgtccgt gctttcccag cagcggattt
    16621 cgatctgatt gccgaagacc cgcttgccgt cgcgcatgcc ggtgacgcgg tcttcatgca
    16681 ggatcgccag atactggaaa gccttcgtgt ccttgagcgc cgcttcggtg atggcggtcg
    16741 ctttcctgac cagggcgatt tttgcgggcg taacttcgcc gatcacgcgg gcggccagcg
    16801 ccggtcccgg gaaaggaatc cggtcgaaca tggcggaggg aaggcccagg cccgccccgg
    16861 ctttgcgcac gccgtccttg cgcagctcga tgagcggttc caggatcctg tagccgaaag
    16921 ctttttgcgg gtcgatgccc agttgttcga aaacgttgtg ctggcgcttg atgccggcga
    16981 cggtttcgtc cacgtcggtg aggatggtgc cctgcaggag atattgggct ttgcttttga
    17041 cgaccagctt gccgaagaca tttttgtaga aggtctgggt aatggcttct cttttttcct
    17101 cggggtcggt gaggcccctg agggcggcaa agaaggcttt ggaggcgtcg atgacttcca
    17161 cggtgatgcc cagttttctg aaggccgcct gcacccgggc cggttctcct tcccgcatga
    17221 tgccgttgtc gatcatgtag gttttgaggc gattgcccag cgccttgtgt cccagggcgg
    17281 tgacgacgca ggaatccacg ccgcccgaga gcgcattgac ggcaaggccg ttgcctacgg
    17341 cgttgcggat atcccgaact ttatcgtcga taaatttctc cacattcatg gcgctggctt
    17401 tgatttcttt catcttcatt ttgacggctc ctttgcggta aaataggttg ctgtttgttt
    17461 aaatgaaatt ccggcacgtt tcaagcatta agattaaaaa acgaaatcgt ttgccccggg
    17521 gcaagcttca acattttgat tgtaaatttg gcaggttcgg tttagaaaac atcccgtttg
    17581 acgggcgaag ctggcgcaaa ggctgccccc ggaaaggaga gttgaatgga gccgattaaa
    17641 atcatgccct gcctggatat gaaaaacgga agggtggtaa aaggggttca ttttgttgac
    17701 attcgtgagg cgggcgaccc ggtcgagaat gccgtgttct atcaggccga aggcgcggat
    17761 gaactggcca tgctggacat tgccgccacg ctcgaaaacc gcaagacacg gctggaatgg
    17821 gtgaagaagg tttccgccca gatcagcatt ccgctgacgg tcggcggcgg tatttcctcg
    17881 ctggaggaca tcgaaatggt gctgggcgcg ggcgcgagca aagtgtccat gaacagcgcg
    17941 gcggtgaaaa atcccgaact ggtcaggctg gcgtcggcaa aattcggtcc ggaaaaaatc
    18001 accgtagcca tcgacgccaa gagaaatccg gcgatgtcgt cgggctttga actggtggtt
    18061 tccggcggca cgctgcccat cgcgaaagac gcggtggcgt gggccaggca atgccaggaa
    18121 ctgggcgcgg gcgtgatcct gcccaccagc atggacggcg acggcacaaa ggcgggatac
    18181 gatattccct tcaccaaagc gatttcctcc gccgtcagcc tgccggtggt ggcgtccggc
    18241 ggcgcgggaa agctggagga tttttacgaa gcggttgtgg acggaggcgc gtccgttctg
    18301 ctggcggcgt ccgtttttca tttccggctt ctcaaaattc aggacgtcaa agactttctg
    18361 aaaagcaagg ggcttgccgt tgcctgaacc ccgggcaagc ggacgtcacc ggtagaagtc
    18421 aaaaatgatt aaatgggctg atattatttt ttttcgcaag cacggggttt gcccccgctg
    18481 gttctgcttc acgtttgaca acggtttgcg ccgcctggtt caaaatccgg accggatcat
    18541 ccgtccttac gttggggaag gggacaaggt gctggatgtg gggccgggca tcgggtattt
    18601 tacgattccg atggcccggc gcgtgggttc aggcgggaga gtcatcgccg ccgatattca
    18661 ggaatcgatg ctgcggggca ttgtcagacg cgccgaacgg gcgggagtgc gcggccaaat
    18721 cgagctttat ctttcccggc ccgacgaaat ctattccgga gagccggtgg atttcattct
    18781 gtccttctgg atggcgcatg aagttccacg gctggaccgg tttttccgtc agttgcgcgc
    18841 cgctctcaaa cctgcggccc gcttcctcct ggcggagccc aaactgcacg tgtcacggac
    18901 gcagtttgac ggggaaattg ccgcggcgca aaaggcggga ctggcgctat tggaacgtcc
    18961 tgcaatttct ttaagccacg gcgctttgtt tggaagcggt cgcgtcggta cgagtggacg
    19021 ctttccggcg gatcagaggg aagcgtcgcc ggtcagcaga aaggagcatt aatgacgctt
    19081 atgaaaaatc gggtgtggac aggaatcatt tttctcattt tgatgcctct gtgggcctgg
    19141 gccgcggagt atgacaccgg tgtgcagagc cgggtgattc ttcaggcgga aacgatgagc
    19201 aatggggagc cgatcgatta tctggacgcg gaccggcctc aggtgaccgt catgacggtg
    19261 gacattgcgc ccggcgcaag cacgggctgg cacagccatc ccatgcccgt ttacgcctat
    19321 gtcatggccg ggcaactgac cgtcgagatc gaaggaggaa agaccgccga attcaaagaa
    19381 ggcgatgcga ttatcgaagt cgtcaaactc aaacacaacg gaaccaacca tggaaaggtt
    19441 ccggtcaaac tcgtggtttt ctacctcggc gcaaaagacg tttccaatgt tattcgggcg
    19501 gaccgtccct gacctgcttt tcctttccta ctccccgatg attttcacca gtactctctt
    19561 ctttctcccg ccgtcaaatt cgccgtaaaa aatctgctcc caggggccga agtcgagctt
    19621 gccttttgtt acggccacga cgacttccct tcccatgacc tgacgtttca gatgcgcgtc
    19681 gccattgtct tcgccggtgc ggttatgccg gtaaaaggat gtgggcgcgt gcggcgccag
    19741 ccgctccagc cagtcctcat aatccgcgtg gagacccggt tcgtcgtcgt tgatgaacac
    19801 ggacgctgtg atgtgcatgg cgttgaccag agcgaggcct tcctgaacgc cgcttttttt
    19861 cagcactgct tcgacgttgc cggtgatgtt gacaaaagcc cttctccggg gaatatcaaa
    19921 ccataattct tcgcggaacg acttcataat ataaaacctc cggtttgaat atgtaattaa
    19981 ctttacaagc ttatcgcctt atgctagaag gcaaaaaaat atgcaagatc aacttttttg
    20041 tcaggagag
//
LOCUS       DAOSRM010000035        19918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2891_length_19918_cov_102.238635, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000035 DAOSRM010000000
VERSION     DAOSRM010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 19918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19918
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2891_length_19918_cov_102.238635"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..850
                     /gene="budA"
                     /locus_tag="PLA58_05025"
     CDS             149..850
                     /gene="budA"
                     /locus_tag="PLA58_05025"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOR62190.1"
                     /translation="MPSMLYISSPINALLEGFYRDDITFERLKEKGDFGIGTFNHLDG
                     EMIALNGSFFRLDLDGNAHPVDGQMKTPFAVLCRFKPMLTEEIFGPLSCEAFMERLRH
                     ILPSDNMFYAIHMAGSFASVETRSVPRTENYRPLAEATHNQKIRHFENVEGHLVGFYT
                     PSFVPSVSVPGYHFHFIDRTLSTGGHLLRCEPNHLDIQMQIFFSMELTLPKTLDYLTV
                     SFERNAREDLEKAER"
     gene            1055..2416
                     /locus_tag="PLA58_05030"
     CDS             1055..2416
                     /locus_tag="PLA58_05030"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOR62191.1"
                     /translation="MNNANKKREHWATRAGFVLATAGSAVGLGNIWRFPYMTGEHGGA
                     AFILIYLVAVLLLGYPLVVNEMVLGRAAQRDPVGVFKVLAPNSPWWLVGALGVFTGFI
                     ILSYYAVVAGWSLAYIYKVVVATVVPGIDHARVFQDHVGSVWEPILWQALFLILTVGI
                     IAAGVVKGIQRWSMVLMPVLFVLLLVLIVRSVTLPGAGEGLAYYLRPDFSKVTGRTLL
                     AALAQAFFSLSLGMGAIMTYGSYLDDKDEIPSNAMMVGGLDTGVALLAGFALFPAIFA
                     LGFEPGAGPGLAFITLPAVFAQMPLGAFFGVLFFILLGIAALTSAISLLEVVTAWLMD
                     EKDWSRKRAAVVMGLVIFIVGLPATLGYSVLKDFSFPGLNKDLLDTYDWISNSILLPV
                     GGFMMAIFTGYVWGAKHAIAEVNKGHKRFMIGQGWALLIRYVVPVLIFFIIVVGIYDT
                     FFR"
     gene            2448..4040
                     /locus_tag="PLA58_05035"
     CDS             2448..4040
                     /locus_tag="PLA58_05035"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOR62192.1"
                     /translation="MIPRSGVRNNLFNAQDLRRIRALGLRPSDVKKQLEAYRRGFRSL
                     TLVRPCVPGDGIRSLSPAERRRLIRLYDAEASGHKMVKFVPASGAASRMFAGWYAAAD
                     RGTFGNDGLDRSFFRDLDRMPFVSLLKQNDGTRRMLRKKDVRAVLQSILLPDGLNFSG
                     LPKALISFHAYRGGEVRTALEEHLREAADLIADRRGVCRLHFTVSAEHVQPVKSFLRL
                     MVPRLEESCGVRFHVDLSLQSPSTSIVAADGQSRPFRDEDGGLVFRPGGHGALLQNLQ
                     AIDADLIFVKNIDNISPESLQKKILPCKKALGGLMIETRRQVYSCLRQLEQKSLSLPD
                     LQAISEFCRDALSADFPKEFHKLAAREKKQWLFRRLNRPLRVCGMVKNEGEPGGGPFW
                     VLEKDQSRTLQIVESAHVDFSLPQQAGIWSSASYFNPVDMVCCTKNYRGGKFILEDFV
                     NRDAYLISPKTRKGRELLAQELPGLWNGGMALWNTVFVELPLAVFNPVKTVYDLLRPQ
                     HRTGRAFKSSSAGTGPAKRPAR"
     gene            complement(3991..4965)
                     /locus_tag="PLA58_05040"
     CDS             complement(3991..4965)
                     /locus_tag="PLA58_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOR62193.1"
                     /translation="MSIKALALFSGGLDSILAVRLVQEQGIEVAGLTFTTPFFNAHKA
                     RAAAEQIRLPLTVEDITGEHLLMLKSPRYGYGKNMNPCIDCHTLMLKIAGRKMEQTGA
                     DFLLTGEVLGQRPMSQTRQSLHVVAKNSGHADSILRPLSAQLLDPIKAEREGKIDRSH
                     LLAISGRGRKEQMRLAETFGILHYPPPAGGCLLTDPMFARRLRDLMLHGEDRGIRDYE
                     LLKYGRHFRVPETGKIIVGRNQSDNEALRGLAAEDDLVCLMADFPGPFVLAPGGSEAA
                     VAVAASLCVRYSDAPADREALVSCRRRGKSAIITARAVSQDLSQRWMI"
     gene            complement(5048..6826)
                     /locus_tag="PLA58_05045"
     CDS             complement(5048..6826)
                     /locus_tag="PLA58_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOR62194.1"
                     /translation="MRHSFLPAGVCVILSAMTFCFQAISAQEDSAQLTFRKTAVSKKS
                     VQVHTVRENEAIYGIIRNLPGVAEKDIPRYFQIIRALNPQVKNWDKIYPGQKITLPST
                     SSAGARKEESAAATEGLSSGASGYRNYRVKKGEYLIRIVHRELNIHSNTQQAMLKIKE
                     MNPSIRDANLLYPGQIIRLPKGSIQAKTVVSTPQMDDPPHAPATLPEEQSAQTGEARP
                     SAAMMPEDSLAQTEKAASGKMSSPLSSGTKLEIIRHIVTQMQGTLTTKGNYYLPVSKT
                     AQLTIDCSAIPVLELNDQTTVFLDMKNRSDVPLRKLIGSRWKNWHIVKIDAKDDVIVM
                     LKKIFEKSKSYELIRAEKPLAVGSPPDVEIPVDWLINRKTAAQPPKTVQGLRLVYHNQ
                     DVLPPAITHFARANSLAITEFSPRTGLAGRPEELYHLSPMTVLPKSPAGELGHALLSY
                     LKLPAEKDAEIRVFNLEKDGFNLSIKADILLARGEKKTVVLSRTLPEEFIHVLRKSGY
                     ELIFVSDQDNPATTLEKILRGGNVPFASGYFLFSGTDKNQPPYTIGFQGIKVKADRDI
                     YVVNFDVHPDLLGLLKEAWSATLVRY"
     gene            complement(7162..8031)
                     /locus_tag="PLA58_05050"
     CDS             complement(7162..8031)
                     /locus_tag="PLA58_05050"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOR62195.1"
                     /translation="MGCKPKPEDIAGGQAILEGVMMRHGDKIAAAVRRPDREIVFQER
                     RYVPLTKRYKPLGWMFVRGTVSLFEMMIIGIQCLMFSAEVALSEEEKKPQGWEMYVSF
                     AISFTVAIFFFVVVPAFFFTKVKIYIDSLILLNILEGCVRLGIFLCFLAATLLMADMR
                     RVYMYHGAEHKTVFAWEDGQDLTVENVKKYSTRHPRCGTSFILFVMIVSILVFSLLGR
                     PDFLHRVIYKILLMPVIAGISYEAIRFTGKYGRFKLVRMLSWPGLALQKITTREPDDD
                     QLEVAIAAMKKVI"
     gene            8183..8404
                     /locus_tag="PLA58_05055"
     CDS             8183..8404
                     /locus_tag="PLA58_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62196.1"
                     /translation="MAPMDASTAAFIKEYQERFEKKLKENEIALLEHWRAQLDKIESA
                     RPDSIASLQLQIRKLSEMMANRIKVLKKG"
     gene            8406..8984
                     /locus_tag="PLA58_05060"
     CDS             8406..8984
                     /locus_tag="PLA58_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR62197.1"
                     /translation="MESIADFLFEVGMLSKTPRSGYQFLGSGRESVAEHILRTVFIGY
                     ALCKLNPSLNELRVLKMCILHDLPEARTGDMNYVNKKYVEVNEAKAVRELTEGLFFGG
                     DIRQAIDEFNARETPEAKTARDADQIALILQLKEYGDLGNKYSDEWIRYALQRLATEE
                     GKKLADRIIQTDSSHWWFKDKSDWWVNGSNRA"
     gene            9098..9448
                     /locus_tag="PLA58_05065"
     CDS             9098..9448
                     /locus_tag="PLA58_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62198.1"
                     /translation="MTSPDEYRHVYEAKEKYILKAVAGVLEEKKIGRNVAIDYDNNRV
                     DSDFMISGDWRTKTNARVKRINWKECEVTLIVTTEKKTETGWEMRRLLQKEQYDTFFS
                     VIELKVYEEMSRMY"
     gene            9472..9867
                     /gene="mscL"
                     /locus_tag="PLA58_05070"
     CDS             9472..9867
                     /gene="mscL"
                     /locus_tag="PLA58_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOR62199.1"
                     /translation="MAPKPKVSALVEEFKNFALKGNVVDLAIGVIIGAAFGKIIDSLV
                     KHIIMPLISLIMPGEQGYLAWKWVVNGKEIPYGLFIGEVVNFLIIALALYIFIVKFIG
                     MIVKSKEAAPPAPTKDQELLAEIRDLLKK"
     gene            complement(9947..10129)
                     /locus_tag="PLA58_05075"
     CDS             complement(9947..10129)
                     /locus_tag="PLA58_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62200.1"
                     /translation="MATIMPEAENVQKAIKWISGSLEDGEGQPLHVLIEKAVFKFDLS
                     PKDSEFLLGFFRNHKK"
     gene            10358..10936
                     /locus_tag="PLA58_05080"
     CDS             10358..10936
                     /locus_tag="PLA58_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOR62201.1"
                     /translation="MAKSIKGTQTEKNLLASFAGEAQARNRYTFFASAAKKEGYEQIS
                     RFFLETAENEKEHAKVFFKYLEGGELEITAAYPAGVIGDTKSNLEAAAAGENMEWTVL
                     YADAAKIAREEGFPEVARSFEQIAKVEKFHEERYRKLAANIVKGEVFAKKAATKWHCI
                     NCGYVYEGAEAPKECPACRHPQSYYEVLAENY"
     gene            11309..11941
                     /locus_tag="PLA58_05085"
     CDS             11309..11941
                     /locus_tag="PLA58_05085"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOR62202.1"
                     /translation="MSTKKQRRSENFKDRQKKKSTRIAYLAAIIGGIVLLLVLFFVIL
                     FNALFPPVDMEALNRKKISAKIYFSDPLERFLVPEKRVIYKEEDPAQQAGEVIRALLE
                     GSKTKSKTGNVNTFPAGVVFKDIRLDKDGTAQVNFGAGLTKNHPGGSTAEMMTIYSLT
                     NTLTENVPQIQAVKILVDGKELPTIKGHISTTHPFRPDPDLYAPAKEEKS"
     gene            11949..13982
                     /gene="fusA"
                     /locus_tag="PLA58_05090"
     CDS             11949..13982
                     /gene="fusA"
                     /locus_tag="PLA58_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR62203.1"
                     /translation="MAPKSKISRTRNIGIVAHIDAGKTTVSERILFYTGKSYKMGEVH
                     DGEAVMDWMPQEQERGITITSAVTTCTWKNTEIHIIDTPGHVDFTIEVERSLRVLDGA
                     VVVFCAVAGVEPQSETVWRQADKYGVPKIAFVNKMDRIGADFFHVVEMMRDRFGSLPV
                     PVQIPVGSEDTFQGVVDLINRKLLIWDETSQGADFTVGEIPEDLTQAASLEREKMIET
                     LADCDDELAEIYLAGEDATPAQIITALRKATIALKIVPVLCGAALRNKGIQPLLDAVI
                     QYLPSPEDIPPVEGINPKTRQQEIRRSSDKEPLAALAFKVQMDEGRKLTYLRIYSGQI
                     SSNGEVYNAVKKKKEKIARLLQMHSNKRERIDRASAGDLVAVLGLKETTTGDTLCDEA
                     RPIVLEQMEFYEPVISQAIEAKTPADQEKLAVALAKLTEEDPTLKVKYEDETAQTVIS
                     GMGELHLEIIVDRLHREFNARVNVGRPRVVCRETIQRHAEAEYLFEKELGDKKHFGQV
                     RLELTPRKRGAGNEIVNAIPEEIIPAEYHEAIEESIRDAMTSGVLNSYPVIDVSARLT
                     GGAYREGEASIQGYKIAAATAFRDACLAAEPIMLEPIMMVDIITPSEFMGDVIGDINA
                     RRGEIQAVNPKGPVSEIKAKVPLKALFGYSTDLRSATQGRAVFTIMFEEYNKA"
     gene            14087..15466
                     /locus_tag="PLA58_05095"
     CDS             14087..15466
                     /locus_tag="PLA58_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOR62204.1"
                     /translation="MSVLKHAWERVRDYAIFESERDAYPQSFSEELNEQCGRILFVVS
                     LITSVAWLPYIYLDAMLHPEEPLLPVIRIGLSVFSVIIFILYISKRFLQYNMLFLVLI
                     GAYLAIGCGLITALADADPVYVGGYIFILTVMALVPVDRRYALVIIAASLLTFFVVGR
                     LENIDFNRPSDRYSLQDLSSATVVAIAFIYLMHNTRLASWLKSRKIEQQSEEMRSDKE
                     KIDNLLLNILPPVVAEELKGQGYVKPVFYPAATIVFTDFVGFTKISEALTPDQLVREL
                     DSAFSRFDRVMDKYGLEKLKTIGDSYMYAGGVPLPNATHAIDAVLGALEIRSFVEQIN
                     LEKSQSGRPVFEIRIGINSGPLMAGVVGEKKFVYDVWGDSVNLASRMESSGGKGRINI
                     SESTYARIKDYFETEARGQVLAKNKGAVGMYFVKGLKKEYASDPEGRTPNERFQAIYR
                     KIQEENITP"
     gene            complement(15609..16916)
                     /gene="trmFO"
                     /locus_tag="PLA58_05100"
     CDS             complement(15609..16916)
                     /gene="trmFO"
                     /locus_tag="PLA58_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOR62205.1"
                     /translation="MIIGGGLAGCEAAWQLLRRGHRVDLYEMKPRRFSPAHKTERLAE
                     LVCSNSLKSNQPDSAPGLLKEEMRRLNSLILAAADQTAVAAGSALAVDRNKFSLRVEE
                     QLLAQEGFSLHRAEVTDIPQDGLTIIATGPLTSDALAHSISRMLGGNSYLYFYDAIAP
                     IVEADSIDMDKAYWASRYGRGTPDYLNCPLSREEYRRFREALLAGRKVEARPFEDVRH
                     FEGCLPIEILAARGEETLAFGPMKPVGLIHPQTGQMAYAVVQLRRENSGGTLFNLVGF
                     QTKLTWPEQERIFRMIPGLEHARFARMGSIHRNTYIHSPSLLLPSLQFRGHPDIFFAG
                     QISGVEGYMESTAMGLLAGLNAASILEGKSPLPPPRETAIGALARYITSSESADNFQP
                     MNIHFGILPPLNAPRMKKKEKHLFCVRRALEALADWVRDQGLS"
     gene            complement(16931..19264)
                     /gene="topA"
                     /locus_tag="PLA58_05105"
     CDS             complement(16931..19264)
                     /gene="topA"
                     /locus_tag="PLA58_05105"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOR62206.1"
                     /translation="MANSLIVVESPTKVKTIKKFLGKDFHVLASMGHIKDLPKSSLGI
                     DLEKDFEPTYHVIETKKKTIADLKKAAKDAENIYLAPDPDREGEAIAWHIADVVDAKK
                     NKKVYRVLFNDLTKKTVLEAIQHPLPLDFDKYEAQQTRRILDRLVGYQISPILWDKVK
                     RGLSAGRVQSVAVRIICEREEEISRFVPEEYWNLVAQFEAGAPPPFDAKLIKIGGKKA
                     KVSNAEEAGRLAADIRSAEFTVEKLEKKEVKRSALPPFTTSKLQQEASRWLKFSAKKT
                     MSVAQKLYEGIELGKEGSVGLITYMRTDSYRIAEEPLAAVRSYIKENYSDAYLPQKPH
                     VYKNAQKAQDAHEAIRPSSMAFKPQDIKPYLTPDQFRLYQLIWNRFVASQMNPAVFDQ
                     TTIDIAGADCTFRAQGQVMKFPGFTIVYTEGKDEKEEENGTDKLLPDVRENEIIKLIK
                     LDTQQKFTQPPPRFSEASLVRELEENGIGRPSTYAAILSTIQDREYVRLEKGKFFPTE
                     LGKIVTELLVQSFPTILDMAFTADMENKLDAIENGANQRVRTLQDFYGLFSGELSKAK
                     KGMRNVKLEETPTDLVCEKCSSPMVIKWGKNGRFLRCSKYPDCKNTRNFTHDENGKVQ
                     HMEPQTTEVKCNKCGKNMVVKEGRFGQFLACSGYPECKNTMNATVNENGDVVAQEAPH
                     TDEVCELCGKPMAVKRGRYGQFLGCTGYPECKNIKKLGKDGKVTQKAQEVLSDEVCDL
                     CGKPMAVKRGRYGQFLGCTGYPECKNIKKIPRKKSDE"
     gene            complement(19323..>19918)
                     /locus_tag="PLA58_05110"
     CDS             complement(19323..>19918)
                     /locus_tag="PLA58_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOR62207.1"
                     /translation="QGAVISEYPPGTQPLPFHFPARNRIISGISYGVLVVEAGEKSGS
                     LITAKLAMEQGREVFAIPGPIDSASSRGANHLIKQGAKLIDSVDDILEEILPQLERTA
                     REPVSPDRKATQTGSEQPYPQKNGVPERAVLEKNVETLLPTDRKILKAIADGKTHADD
                     IIQETGIPPAEAMGALMRLELQGRVLQHPGKLFTLKK"
BASE COUNT         4393 a         5320 c         5592 g         4613 t
ORIGIN      
        1 cctccaccaa aaaaatcagt tacttgcgcc attttctcct tgtcgttttt tatcaggggg
       61 aagggggcca cttaattgag gccgatggta aaaaaaaccc gcgtacagcg ggtttttctt
      121 aaacaggcgg caaaaagcgg agcacatcat gccatccatg ctttacatca gcagccccat
      181 taatgcattg ctggaaggat tttaccggga tgatatcacg ttcgaacggt tgaaagaaaa
      241 aggggatttc ggcattggca cgttcaatca cctggacggg gaaatgatcg ctctcaacgg
      301 ttcctttttc cggctcgatc tcgacggaaa cgcccatccc gttgacggac agatgaaaac
      361 cccgtttgcc gttctttgcc gtttcaagcc catgcttacc gaagaaatct ttgggccgtt
      421 atcctgcgag gcattcatgg agcgtttgcg gcatattctt ccgtccgata acatgtttta
      481 tgccattcac atggcaggtt cgtttgcgtc ggtcgagaca cgctctgttc cgcgaaccga
      541 aaattaccgg ccgctggcgg aagcgaccca caaccagaaa atccgacatt tcgaaaatgt
      601 tgaaggccat ctggtcggat tttatacgcc ttcctttgtt ccgtccgtca gtgtgccggg
      661 ataccatttt catttcatcg acaggaccct ctcgacgggc ggccatctcc tgcggtgcga
      721 acctaaccat ctggacattc agatgcagat ttttttcagc atggagctga cgctccccaa
      781 aacgctggat tacctcacgg tgtcctttga gcggaacgca cgggaggatt tggaaaaggc
      841 ggaacgctga ccatttaaaa atgcgtcagg gttcactttc aatgcttttt ccggttgatc
      901 cgtcattgga tgccgggttt cccggtttta tgaaatcctt gcccggctcc gtcctcttct
      961 acggttttgc cttgacaccc cgcatggaaa ttcctatagt tcgcggcgac aaaacgtctt
     1021 ccgcggaaaa agcgcgtgga ccgggaaaaa accaatgaat aacgcaaaca aaaagaggga
     1081 gcactgggca acgcgggcag gttttgttct tgctacggcg ggatcggccg tcggcctggg
     1141 aaacatctgg cgtttcccct acatgacggg cgagcacggc ggcgcggctt tcatattgat
     1201 ctacctggtt gccgtcttgc ttttgggtta tcccctggtt gtcaatgaaa tggttttggg
     1261 ccgggcggcc cagcgggatc ccgtgggggt ttttaaggtt cttgccccga acagtccctg
     1321 gtggctggtg ggggcgctgg gtgttttcac gggtttcatt attctttctt actatgccgt
     1381 tgttgccggc tggtcgctgg cctacatata caaagtcgtt gtcgccacag ttgttccggg
     1441 aatcgatcac gcccgtgttt ttcaggatca cgtcggcagc gtatgggagc cgattctatg
     1501 gcaggccctg tttctgattc tgacggtcgg catcattgcc gccggtgtcg tcaaaggcat
     1561 ccagcgatgg tccatggttc tgatgcctgt tctcttcgtg cttttgctgg tcctgatcgt
     1621 ccggtcggtg accttgcccg gcgcgggcga aggtcttgcc tattacctgc gccctgattt
     1681 cagcaaggta accggcagga cgttgctggc cgctctggcg caggcgtttt tttctttgag
     1741 tctggggatg ggcgccatca tgacctatgg aagctatctg gacgacaagg atgaaattcc
     1801 gtccaacgcc atgatggttg ggggactgga tacaggcgtg gcattgctgg cgggctttgc
     1861 cctgtttccc gctatttttg ccctggggtt cgaaccgggc gccgggccgg ggcttgcctt
     1921 tatcaccctg ccggccgttt tcgcgcaaat gcctctgggc gcttttttcg gcgtcctttt
     1981 tttcatttta ctgggcattg ccgcactgac gtccgccatt tccctcctgg aggtggtgac
     2041 cgcctggctg atggacgaaa aagactggtc caggaaaaga gccgctgttg tcatggggct
     2101 ggtcattttt atcgtggggc tgccggcgac gctgggatac agtgtcctga aagacttctc
     2161 tttcccggga ttgaacaagg atctgctcga cacctacgac tggatttcca acagcatctt
     2221 gctgcccgtg ggaggcttca tgatggccat tttcaccggc tatgtgtggg gcgccaaaca
     2281 cgcgattgcc gaagtcaata aaggccataa acggttcatg atcggccagg ggtgggctct
     2341 gctgattcgg tatgttgttc ccgtactgat tttcttcatc atcgtggtgg gaatctacga
     2401 cacgtttttc agataaacgg cctgccgccg aacaggaggt gtaacgcttg atccctcgtt
     2461 ccggtgtgag gaacaatctt tttaacgcgc aggatctgcg ccgcatccgg gcgcttggcc
     2521 tgcggccgtc cgacgtgaaa aaacaactgg aagcctaccg gcgcggcttc cggtctttga
     2581 cgcttgtgcg cccctgtgtc ccgggtgacg gcatccgttc cctgagcccc gcggaacgca
     2641 ggcgcctcat tcggctgtat gacgccgagg cctccggaca caaaatggta aaatttgttc
     2701 ccgcatcggg cgcggccagc cgcatgtttg ccgggtggta tgcggcggcg gaccggggga
     2761 ccttcggcaa tgacgggctg gaccggtctt tttttcgcga tttggatcga atgccctttg
     2821 tttcccttct gaaacaaaac gacggcacgc gccgaatgtt gagaaaaaaa gacgtccggg
     2881 ccgttcttca aagcattctt cttccggacg gcctgaattt cagcggtctt cccaaagcgc
     2941 tcatttcctt tcacgcctac cggggaggag aagtccggac ggcgctggag gagcacctgc
     3001 gtgaagcggc ggacctgatt gcggacagga gaggggtctg tcggctgcac ttcacggttt
     3061 ccgccgagca tgtccaaccg gtaaaatcgt ttttgcgatt aatggttccc cggctcgaag
     3121 aaagctgcgg cgtccgtttt cacgtggatt tgtccctgca gtccccgtcc acgagcatcg
     3181 tggccgcgga cgggcaatcc cggcctttcc gtgatgaaga cggtggcctg gtcttccggc
     3241 ccggcggaca cggcgctctt ttgcaaaacc ttcaggccat cgacgccgac cttatttttg
     3301 tcaaaaacat cgacaatatt tcgccggagt ctctgcaaaa gaaaattctg ccctgcaaaa
     3361 aagcactggg gggattgatg atcgagacgc ggcggcaggt ttattcctgc ctgcggcaac
     3421 tggaacagaa gagtctttcc ttgccggacc ttcaggctat ctcggaattc tgccgcgatg
     3481 cgctgagcgc ggactttccc aaagaattcc acaagctggc ggcccgggaa aaaaaacagt
     3541 ggctgtttcg acggctgaac cggcctttgc gcgtgtgcgg catggtcaaa aacgaaggag
     3601 agccgggcgg cggacccttc tgggttctgg aaaaagatca atcccgaact ttgcagatcg
     3661 tggaatccgc ccatgttgat ttcagccttc cccaacaggc gggcatttgg tcgtcagctt
     3721 cttatttcaa tccggtcgat atggtctgct gcacaaaaaa ttatcgcggg ggaaaattta
     3781 ttctggagga cttcgtcaac cgggacgcct atctgatcag ccccaaaacc cggaaagggc
     3841 gcgaacttct ggcgcaggaa ctgcccgggc tctggaacgg gggcatggcc ctctggaaca
     3901 cggtttttgt cgaacttcca ctcgcggttt tcaatcccgt gaagaccgtt tacgatcttc
     3961 tgcgtccgca gcaccggacc ggaagagcct ttaaatcatc cagcgctggg acaggtcctg
     4021 cgaaacggcc cgcgcggtga tgatggcgga ttttccccgg cgcctgcacg aaacgagggc
     4081 ttccctgtct gccggggcgt cggaatagcg gacgcacaga gaggcggcca cggccacggc
     4141 cgcttcgctg ccgccggggg ccagaacaaa cggaccggga aagtcggcca tcaagcagac
     4201 caggtcgtcc tccgccgcaa gaccccgcag ggcttcgttg tcggactgat ttctgccgac
     4261 gatgattttg ccggtttcgg ggacgcgaaa gtgccgtccg tatttcaaaa gctcataatc
     4321 ccggattccg cggtcctcgc cgtgcaacat caaatctcgg agacgccttg caaacatcgg
     4381 atccgtcagc aggcagccgc ccgccggcgg cgggtaatgc aagattccga aagtttccgc
     4441 cagacgcatt tgttccttcc gtccgcggcc tgaaatggcc agcaggtgcg accgatcgat
     4501 ctttccttcc cgttccgcct tgatcggatc caacaattgg gcggaaagcg gccgcaggat
     4561 ggaatcggca tggccggaat tcttcgcgac cacatgcaga gactgtcgcg tctgggacat
     4621 ggggcgctgg cccagcactt cgccggttaa caggaaatcc gcgccggttt gttccatttt
     4681 cctgcctgcg atcttcagca tcagcgtatg gcagtcaatg cagggattca tgtttttgcc
     4741 gtagccgtag cgcggggact tcagcattag cagatgttcg cccgtgatgt cttccaccgt
     4801 gagaggcagc cggatctgct ctgccgccgc ccgggccttg tgcgcgttaa agaagggggt
     4861 cgtaaaggtc aggccggcaa cctctattcc ctgttcctga accagccgga cggccagaat
     4921 gctgtcgagc ccgccggaaa aaagggcaag cgccttgatg ctcatggaat tcctctttca
     4981 taaataaaaa acgcggcaga aaagcttccg cggcgctttc gtgtgatccg ttaaccggcg
     5041 cggagcgcta gtaccggacc aaggttgccg accacgcttc tttcagcagc ccgagcaggt
     5101 ccggatggac atcaaaattc accacataaa tatcccgatc ggccttgacc ttaatgccct
     5161 ggaatccaat ggtataaggc ggttgatttt tgtctgtgcc ggaaaataaa aaatagccgg
     5221 acgcgaaggg gacattcccc ccccgaagaa tcttttccag ggttgtcgcg ggattgtcct
     5281 ggtcggatac aaaaatgagc tcatagccgg atttccggag gacgtgaata aattcctcgg
     5341 gaagggtgcg ggataaaacc accgtcttct tttcaccccg cgccaacaga atatccgctt
     5401 tgatcgacag gttgaatccg tccttttcaa ggttgaatac ccggatttcc gcgtcctttt
     5461 cagccggcag cttcagatag gaaagcaggg catggccaag ttcaccggcg ggcgatttcg
     5521 gcaaaaccgt catgggggac aggtgataaa gctcctccgg ccggccggca aggcccgtgc
     5581 ggggagaaaa ttccgtgatc gccagggaat tggcccgggc aaagtgcgtg atggccgggg
     5641 gcagaacgtc ctggttgtgg taaaccagcc gcagtccctg gaccgtttta ggaggctgtg
     5701 cggccgtttt ccggttgatc agccagtcca cgggaatttc cacatctggc ggcgatccca
     5761 cagcgagcgg tttttcggct ctgatcaatt cgtagctttt gcttttttca aatatttttt
     5821 tcagcatgac gatcacgtcg tctttcgcgt cgattttgac aatgtgccag tttttccacc
     5881 ggctgccgat cagttttctc aacgggacat cggaccggtt tttcatatcc agaaagaccg
     5941 tggtctgatc gttgagctcc aacacgggga tggcggagca gtcaatcgtc agttgggcgg
     6001 ttttggaaac cggaagataa taattgcctt tggtcgtcag ggttccctgc atctgtgtca
     6061 cgatgtgtct gatgatttcc agttttgttc cggaggacag aggggaagac atctttccgg
     6121 acgctgcttt ttcggtctgt gcaagggaat cttccggcat cattgcggcg gacggccgcg
     6181 cttcgccggt ttgcgcggac tgctcttccg gcagtgttgc cggggcatgc ggcggatcat
     6241 ccatttgggg cgtggacaca acggttttgg cttgaatact gcctttggga agacgaatga
     6301 tctgtcccgg atacaggaga ttggcatccc ggatggacgg attcatttct ttgattttga
     6361 gcatggcctg ctgcgtgttg gaatgaatat tcagctcacg gtgaacaatg cgaatgagat
     6421 attcgccctt cttgacccgg tagtttcggt atccggacgc tcccgatgaa aggccttccg
     6481 tcgcggcggc cgattcttct ttccgggcgc cggcgctgct tgtcgaagga agtgtgatct
     6541 tttgtcccgg gtatattttg tcccagtttt tgacctgggg gttcaacgcc ctgattattt
     6601 ggaaatagcg cgggatatcc ttttcggcaa cgccgggcaa attgcggatg atgccgtaaa
     6661 tcgcttcgtt ttcccggacc gtgtgaacct gaacggattt tttggaaaca gccgttttcc
     6721 ggaaagtgag ctgtgcgctg tcttcctgcg cggagatggc ttgaaaacaa aaggtcattg
     6781 ctgaaaggat tacgcatacc cccgccggca aaaatgaatg cctcatgaac gattgccccc
     6841 gaaaacgata aagataacct ggacgccctc gtaaaaagta attcaaaccg tcactccggc
     6901 gaatacccta accacttgaa ggtggcgaga gggtacaagc gccggagtcc agaacttatt
     6961 gtaatggctg gatgccggcc ttcgccggtg tgacgaaaac ggtgcgaaaa tgacttttaa
     7021 gagaccgtca accttgcgtt ttcaattact tcaagttccc tgaaaagtca atgcccatcg
     7081 ggttgaaaaa ccattggtga gaaagctctt atgcggggac aggttaaaat cacaggagtg
     7141 cgtgttgatg gagaagcgcc gtcagatcac ttttttcatc gcggcgatgg cgacttcaag
     7201 ctgatcgtca tccggttccc gcgtggtgat tttctgcagg gcgagccccg gccagctcag
     7261 catccgcacc agtttgaagc gtccgtattt tccggtaaag cggatggctt cataagaaat
     7321 gcccgcgatc accggcatca gcaggatctt gtaaatgacc cggtgcagga agtcggggcg
     7381 gcccagcagg gaaaaaacca ggatggagac aatcatcacg aaaagaataa agctggtgcc
     7441 gcagcgcgga tggcgggtgg agtacttctt cacgttttcc accgtcaggt cctgcccgtc
     7501 ttcccacgcg aaaacggttt tatgctcggc gccgtgatac atgtaaacgc ggcgcatatc
     7561 cgccatcagc agcgtggcgg cgagaaaaca taaaaatatt cccagccgga cgcaaccctc
     7621 cagaatattt agaagaatca ggctgtcgat atatattttt actttggtaa agaaaaaagc
     7681 cggaaccacc acaaagaaaa aaatggccac cgtaaagctg atggcgaagg aaacatacat
     7741 ttcccagccc tgcggctttt tttcttcttc cgaaagagcg acttccgccg aaaacatcag
     7801 gcattgaatg ccgatgatca tcatttcaaa cagactgacc gtaccccgga caaacatcca
     7861 gcccagaggc ttgtatcttt tcgttaaggg aacgtaacgc cgctcctgaa aaacaatctc
     7921 ccggtcgggg cgcctcacgg cggcggcgat cttgtcgccg tggcgcatca tgacgccttc
     7981 gagaatggcc tgaccgccgg cgatgtcttc aggttttggt ttgcagccca tgcgttgttg
     8041 tttcctgccg tttttttgct gcgctttctt atagtattta accggggctg tcaaatgatg
     8101 cttgaagaaa agtttgttgc atctttcagg aggattgtta tagagtcttg ccgtaatttc
     8161 aggaaatatg gggaggatgc acatggcgcc aatggatgct tcgaccgccg ctttcatcaa
     8221 ggagtatcag gaacggttcg aaaagaagtt gaaggaaaat gaaatagccc tgttggagca
     8281 ctggagggcc cagctcgata aaatcgaaag cgcaagaccg gacagcatcg cctcgctgca
     8341 actgcaaatc cggaaactgt ccgaaatgat ggccaaccgg atcaaagtgt tgaaaaaggg
     8401 ataagatgga aagcattgcc gattttttgt ttgaagtggg gatgctcagc aaaacgcccc
     8461 gcagcggtta tcagtttctg ggcagcggca gggagtcggt ggcggagcat attctgcgga
     8521 cggtttttat cggctacgct ctgtgcaagc tcaatccgtc tttgaacgaa ctgcgcgtct
     8581 taaaaatgtg cattcttcac gatctgcccg aagcccgcac cggcgacatg aactacgtca
     8641 acaagaaata cgtggaagtg aacgaggcca aagccgtccg ggaactgacg gaagggcttt
     8701 ttttcggcgg cgacatccgt caggcgatcg acgagttcaa cgcgagggaa accccggaag
     8761 cgaagaccgc ccgggacgcc gatcagatcg cgctgattct tcagctgaaa gaatacgggg
     8821 atttgggcaa caaatattcc gatgagtgga tccggtacgc gctgcagagg cttgccaccg
     8881 aggaaggaaa aaagctggcc gaccgaatca ttcaaacgga ttcatcgcac tggtggttca
     8941 aggacaaaag cgactggtgg gtcaacggaa gcaaccgggc atagacccat gtcccaaaca
     9001 ggagagatga atgattcata aatcacggtt tttatttttt tcttttttgg cggttctttg
     9061 ccttgcgtcg tgcgccggga aatttggatc ggaggtcttg accagccctg atgaatacag
     9121 gcatgtttac gaggcgaagg agaaatacat tctcaaagcc gttgccggcg tcctggagga
     9181 gaaaaaaatc ggtcggaatg ttgccattga ttacgacaac aatcgggtcg attcggactt
     9241 catgatttcg ggagattggc ggaccaagac gaacgcccgc gtgaaacgga tcaactggaa
     9301 ggaatgcgag gtgacgctga ttgtgaccac ggagaaaaaa acggaaacgg gatgggaaat
     9361 gcgccgcctg ctgcagaaag aacaatatga tacttttttc agcgtgatcg aattgaaagt
     9421 ctatgaggaa atgtccagga tgtattgaag aaaagcacga aaggaggagg catggcgccc
     9481 aaaccaaaag tatcggcatt ggtggaagag ttcaagaatt tcgccttgaa gggcaacgtg
     9541 gtggatctcg ccatcggcgt gatcatcgga gccgcgttcg gcaagatcat tgattccctg
     9601 gtcaagcaca tcatcatgcc gctgatcagc ctcatcatgc ccggcgaaca gggctatctg
     9661 gcctggaagt gggtggtcaa cggcaaagaa attccctacg gacttttcat tggtgaagtg
     9721 gtcaactttt tgatcattgc gctggccctg tacatcttta tcgtgaaatt tatcggcatg
     9781 atcgtgaaaa gcaaggaagc agcgcctccg gctccgacca aagaccagga actgctggcg
     9841 gagattcggg atctgctgaa aaaataagca tttgcggaaa tttctttcag aggaccgcag
     9901 agacggcggc gggggcgtgg cgcatgtcca acgcccccgg ctgtttctat ttcttgtgat
     9961 tgcggaagaa acccaacaga aactcggaat ccttggggga cagatcaaac ttgaaaaccg
    10021 ccttttcgat cagcacatgc aacggctgac cttcgccgtc ttccagactg ccggagatcc
    10081 atttaatcgc cttctgaaca ttttcagctt cgggcataat tgttgccatt ttgttgcttc
    10141 ctccttgaaa tgttttaagc agttttccgc gttggggaat gaatcggcac agcgcaaaaa
    10201 ctttgagaat acatactaca atccgtctga aaaattaaca aatatttgac gccgtttgac
    10261 attaaatttt tggtgattaa gttgttgcag gcaagggttg ataacacact ggaaaaccta
    10321 tacaaattaa aaaaacgaaa ttgaaaggag acgcaggatg gctaaatcaa ttaaggggac
    10381 ccaaacggag aaaaacctgc tggcatcttt tgccggcgaa gcgcaagcca gaaaccgcta
    10441 tacatttttt gcaagcgccg cgaagaaaga gggatacgaa cagatttccc gctttttcct
    10501 ggagacggcg gaaaatgaaa aagagcacgc caaagtgttt ttcaagtatc tggaaggcgg
    10561 agagcttgaa atcaccgccg cctatccggc gggcgtgatc ggcgacacca aaagcaatct
    10621 ggaggctgcg gcggccggag aaaatatgga gtggaccgtg ctctacgccg atgccgcgaa
    10681 aatcgccagg gaggaaggat ttccggaagt agcccgctct tttgagcaga ttgccaaggt
    10741 ggaaaagttc cacgaagagc gctaccgcaa actggccgcc aacatcgtca aaggcgaggt
    10801 gttcgcgaaa aaggcggcga ccaagtggca ctgcattaac tgcggctacg tgtatgaagg
    10861 ggccgaggcg ccgaaggaat gccccgcctg caggcacccg cagtcctatt atgaggtgct
    10921 ggctgaaaat tattaaaacg ccgtcgcgca ggacgaatga tgcaaaagca cgtccgtaag
    10981 gccggcgggc ctggtgcccg ccggcctttt ttatttcagc ttccagagtt atctgacggc
    11041 gaaaggatag cggaattgaa tcccgcttaa gcgggacgag cgttcattct ccgcagcttg
    11101 ctgtgcgata tcatgacgtt cattttatgc ctcgacagct tgccgcgagg tggttgatca
    11161 gagtatccgc cggccggcca gttgtataaa acgcgaactt cataggcgcc gtccggaaga
    11221 atttcattgc ggcaggaagt ttgccgggct tgacactgaa ggaaaaatac cctagattgg
    11281 cgcagttttt ttaagaaagg ttttagtcat gtccacgaaa aagcagcgcc ggtccgaaaa
    11341 tttcaaagac cgccaaaaaa agaaatccac ccggatcgcc tatctggccg ccatcatcgg
    11401 cggcatcgtt ttgctgctgg tgcttttctt tgtcatttta ttcaacgcct tgttcccgcc
    11461 ggttgacatg gaagcgttaa accggaaaaa aatatcagcc aaaatttact tttcagatcc
    11521 tctggaacga tttctcgttc ccgaaaaacg ggtgatttac aaggaagaag atccggcgca
    11581 gcaggccgga gaagtgatca gggcgcttct ggagggatcc aaaaccaaaa gcaaaaccgg
    11641 caacgtcaac acctttcccg cgggtgtggt cttcaaagat attcgtctgg acaaagacgg
    11701 aaccgctcag gtcaacttcg gcgccggttt gacgaagaac catccgggcg gttccacggc
    11761 ggagatgatg accatttatt ccctgaccaa taccctgacg gaaaatgttc cccagatcca
    11821 ggcggtaaaa attctggtgg acggcaaaga actgcccacc atcaaggggc acatctccac
    11881 gacgcatccg tttcgaccgg acccagacct ctacgcgccc gccaaagagg agaaaagctg
    11941 actaccccat ggcgccgaaa tccaaaatat cacgcacgcg caacatcggc attgtcgccc
    12001 acatcgacgc gggaaaaacc accgtcagcg agcggattct tttttatacg ggcaaatcct
    12061 ataaaatggg cgaagtgcat gacggcgaag cggtcatgga ctggatgccc caggagcagg
    12121 agaggggcat caccattacc tccgcggtga ccacctgcac ctggaagaat accgagattc
    12181 atatcattga tacgccgggg cacgtggatt ttaccattga ggtggagcgg tccctgcgtg
    12241 tcctggacgg cgcggtggtg gttttctgcg ccgttgccgg cgtcgagccc caatccgaaa
    12301 ccgtctggcg acaggcggac aaatacggcg ttcccaagat tgccttcgtc aacaagatgg
    12361 accggatcgg cgcggatttt tttcatgtcg tggagatgat gcgcgatcgt tttggctctc
    12421 ttcccgtccc cgtgcagatt ccggtcggaa gcgaggacac gtttcagggt gtggtggacc
    12481 tgatcaaccg caagctcctg atctgggatg agacctcgca gggagcggac tttaccgtcg
    12541 gcgaaatccc ggaggatctg acccaagccg cctcgctgga gcgggaaaaa atgatagaga
    12601 ccctggccga ttgcgacgat gaactggcgg agatttacct ggccggggag gacgcgacgc
    12661 cggcgcagat catcacggcg ctgcgcaagg cgacgatcgc cctgaaaatt gttccggtcc
    12721 tgtgcggagc cgccctgcgc aacaaaggca tccagcccct tctggacgcg gtcatccaat
    12781 atcttccgtc tccggaagat attcccccgg tcgagggcat caatccgaaa accaggcagc
    12841 aggaaatccg ccgcagttcg gacaaagagc cgcttgcggc cctggcgttc aaggttcaga
    12901 tggacgaagg acgcaagctg acgtatctgc gcatctattc cggacagatt tcttccaatg
    12961 gagaggttta caacgccgtc aagaagaaga aggaaaaaat tgcccggctt ttgcagatgc
    13021 attccaataa gcgggaaaga atcgaccgcg cttccgcggg ggatctggtg gccgttctgg
    13081 ggctgaagga aacgaccacg ggcgatacgc tttgcgatga ggcgcgtccg atcgttctgg
    13141 agcagatgga gttttacgag ccggtcatca gccaggccat cgaagccaaa actcccgcgg
    13201 accaggaaaa actggccgtc gcgctggcca aactgaccga ggaagatccg acattaaaag
    13261 tcaagtacga agacgaaacc gcgcaaaccg tcatttccgg catgggcgaa ctgcacctgg
    13321 aaatcatcgt ggatcgcttg caccgtgaat tcaacgcccg cgtcaatgtc ggacgccccc
    13381 gcgtcgtgtg ccgcgaaacg attcaaaggc acgccgaggc cgaatatctg tttgaaaagg
    13441 aactggggga caagaagcat ttcggccagg tccgccttga actgacgccg cgtaagcgag
    13501 gcgccgggaa tgaaatcgtg aacgcgatcc ccgaagaaat catccccgcc gaatatcatg
    13561 aggccattga agagagcatc cgcgatgcga tgaccagcgg cgtgctcaac agctatccgg
    13621 tcatcgacgt atccgcccgg ctgaccggcg gcgcgtaccg cgagggggaa gcgtccatcc
    13681 agggatacaa aattgccgcc gcgacggcgt tccgtgacgc ctgtcttgcc gcggaaccca
    13741 tcatgctcga accgatcatg atggtggaca tcatcactcc cagtgaattc atgggcgacg
    13801 tcatcggcga catcaatgcc cgccggggag agatccaggc ggtcaatccc aaggggcccg
    13861 tctccgaaat caaagccaag gtgccgctca aggccctgtt cggttattca acggacctgc
    13921 gctccgccac acaggggcgg gccgtgttta ccatcatgtt tgaggaatac aacaaggcat
    13981 aaaacggtga tgcaagacca tcaccggcgg atggaataac gtttgtaatt ctgtgaactg
    14041 aacgcctgtc ccgaaagggg cgggtgaaac cctgaaggag gcaaacgtgt ctgttctgaa
    14101 acacgcatgg gagcgggtgc gcgattatgc cattttcgaa agtgagcgcg acgcctatcc
    14161 gcagtctttt tccgaagaat tgaacgaaca gtgcggccgg atccttttcg tcgtttccct
    14221 cattacatcg gtggcctggc tgccctatat ttacctcgac gccatgctcc acccggaaga
    14281 gcctctgctt cccgtcatcc gcataggctt gtccgtcttc agtgttatca tctttatcct
    14341 gtatatttcg aagcgctttc ttcaatacaa catgctgttt ctggttctca tcggcgccta
    14401 cctggcgatc ggctgcgggc tcatcaccgc cctggcggat gccgatcccg tgtatgtggg
    14461 aggctatatt ttcattttga ccgtgatggc tctggtgccc gtggaccgcc ggtatgccct
    14521 ggtgatcatc gcagcgtcct tgctgacgtt ctttgtcgtc ggtcgccttg aaaatattga
    14581 cttcaaccgg ccgagcgacc gctacagcct ccaggatttg tccagcgcga cagttgtcgc
    14641 catcgcgttc atttacctga tgcataatac ccggctggcc agctggctga aatccagaaa
    14701 gattgagcag cagagcgagg aaatgcggtc cgacaaggaa aaaatcgaca acctgctgtt
    14761 gaatatcctg ccgcccgttg tggcggagga gctgaaaggc cagggatacg tcaaacccgt
    14821 gttttatccg gccgcgacca ttgtctttac ggattttgtc ggctttacca agatttccga
    14881 ggcgctgacg cccgaccagc tggtccggga gctcgattcg gccttttccc ggtttgaccg
    14941 cgtgatggac aaatacggcc tggaaaagct caagaccatc ggcgattcct atatgtatgc
    15001 gggcggcgtg ccccttccca acgcgacgca tgccatcgac gcggttctgg gcgctttgga
    15061 aatccggtcg tttgtggagc agatcaacct ggaaaagtct cagagcgggc ggccggtctt
    15121 tgagattcgg atcggaatca acagcggccc gctgatggcc ggcgtcgtgg gggaaaagaa
    15181 atttgtctat gacgtctggg gcgattccgt caatctggcc agccgcatgg aatcttcggg
    15241 cgggaaggga agaatcaaca tttccgaatc cacctacgcc cggattaaag attattttga
    15301 aaccgaagcc cgcgggcagg tgctggcgaa aaacaagggc gctgtgggca tgtattttgt
    15361 caaggggctc aaaaaggagt atgcttcgga tccggaaggc cggacgccca acgaacgctt
    15421 ccaggcgatt tacagaaaga ttcaggaaga aaatatcacg ccatagcgcc cgtcatgatt
    15481 tgagagcttt gaaaaatgtc atttcgagga gcgtgagcga cgagaaacct tcaggatggc
    15541 cgtttgttaa agatttctcc cttcggtcga aatgacaaaa acggccgtta ttcaaagctc
    15601 tcgatttgtc acgaaagccc ctgatcccgc acccagtccg ccagagcctc caaggcgcgc
    15661 cgcacgcaga aaagatgctt ttcttttttc ttcattcttg gcgcgttcag cgggggaaga
    15721 atcccaaaat ggatgttcat gggctggaag ttgtccgccg attcggacga tgtaatatag
    15781 cgcgccagcg cgccgatggc cgtttcccgc ggcggcggga gaggggattt cccttccagg
    15841 atcgaggcgg cgttcaagcc cgccaaaagc cccatcgcgg tggattccat atagccttcc
    15901 accccgctga tctggccggc aaagaaaata tcgggatggc cccggaattg cagcgagggc
    15961 aaaagcaggg aaggggagtg aatgtaggta ttgcggtgaa tgcttcccat cctcgcgaac
    16021 cgcgcgtgtt ccaagccggg aatcatccgg aaaatgcgct cctgttccgg ccaggtcagc
    16081 ttggtttgaa aaccgaccag gttgaatagc gtgccgccgc tgttttcgcg cctgagctga
    16141 actacggcat aggccatctg cccggtttgc ggatggataa gccccaccgg cttcatgggg
    16201 ccgaacgcca gcgtctcttc tcctcgcgcc gccaggattt caatcggcag gcacccctcg
    16261 aagtggcgga cgtcttcaaa cggtctggcc tcaactttcc gccccgccag caacgcttcg
    16321 cggaagcgcc ggtactcttc ccgtgaaagc ggacaattca gataatccgg cgtgcccctg
    16381 ccgtagcggg aggcccagta ggctttatcc atgtcgatgg aatcggcttc cacgatcggg
    16441 gcgatcgcgt cgtaaaaata cagataggaa tttccgccga gcattcggga gatggagtgc
    16501 gccagagcat cggatgtgag aggccccgtg gcgataatcg tcaaaccgtc ttggggaata
    16561 tccgtaacct cggcgcggtg taaggaaaaa ccttcctgcg caagcagttg ctcttcgacc
    16621 cggagggaaa atttgttccg gtccacggcc agcgcgctgc cggcggccac cgccgtttgg
    16681 tcggcagcgg ccaggatcag agaattcagt ctgcgcatct cttctttgag aaggccgggc
    16741 gcgctgtccg gttgattgga tttgagagaa ttgctgcaga ccagttcggc cagccgttcg
    16801 gtcttgtgag ccggagaaaa acgacgcggc ttcatctcgt aaagatcgac ccgatgtccg
    16861 cgccgcagca gttgccaggc cgcttcacag ccggccaggc cccctccgat gatcatgacc
    16921 cgcccggatt tcattcgtcc gatttttttc tggggatttt tttaatattt ttgcattcgg
    16981 gatagccggt gcatcccaga aactggccgt agcggccgcg cttgaccgcc atgggctttc
    17041 cgcacagatc gcagacctcg tcggaaagga cctcttgtgc tttttgggtg actttgccgt
    17101 ccttccccag ctttttgatg tttttgcatt cgggatagcc ggtgcatccc agaaactggc
    17161 cgtagcggcc gcgcttgacc gccatcggtt ttccgcaaag ttcgcagact tcgtccgtat
    17221 gcggcgcttc ctgggcaacc acatcgccgt tttcattgac ggtcgcgttc atcgtgttct
    17281 tgcactccgg atagccggaa caggccagaa actgaccgaa acgcccttcc ttgacgacca
    17341 tgttttttcc gcacttgttg catttaacct cggtggtctg gggttccata tgttggacct
    17401 ttccattttc atcatgggtg aaattcctgg tgttcttgca atcgggatat tttgaacagc
    17461 ggaggaacct cccgtttttc ccccatttga tgaccatggg agaactgcat ttttcacaca
    17521 ccaggtccgt cggcgtttct tccagtttga cattccgcat gccttttttc gccttgctca
    17581 actctccgga gaaaagaccg tagaaatcct gcagcgtccg gacgcgctga ttggcgccgt
    17641 tttcgatggc gtccagcttg ttttccatgt ccgccgtgaa ggccatatcc agaatggttg
    17701 gaaaactttg caccaggagc tccgtgacaa tcttccccag ctccgtcggg aagaatttgc
    17761 ccttctccag gcgcacatat tcccggtcct gaatggtgga aagaatcgcg gcgtaggtgc
    17821 tggggcgtcc gatgccgttt tcttccagct cgcgcaccag cgacgcctcc gaaaaacgcg
    17881 gcgggggctg cgtgaacttc tgctgcgtgt cgagcttgat gagctttatt atttcatttt
    17941 ccctgacgtc cggcagaagc ttgtccgttc cgttttcttc ttccttttca tccttgcctt
    18001 ccgtgtaaac gatggtgaag ccgggaaact tcatcacctg accctgtgcg cggaatgtac
    18061 agtcggcccc ggcaatgtcg atcgtggtct gatcgaaaac ggccggattc atctggctgg
    18121 ccacaaagcg gttccagatc agttgataaa gcctgaactg gtcgggcgtc agatagggtt
    18181 tgatgtcctg cggcttgaaa gccatcgagg aagggcggat ggcctcgtgg gcatcctgcg
    18241 ccttctgggc gtttttatag acatgaggct tctgcggcag ataagcgtcg gaataatttt
    18301 ccttgatgta gctgcggaca gccgccagcg gctcctcggc gatccggtag gaatccgttc
    18361 gcatataggt gatcaggccc acggagcctt cttttcccag ttctatgcct tcgtagagtt
    18421 tctgggcgac gctcatcgtt ttcttggcgg aaaacttcag ccagcgcgag gcttcctgct
    18481 gcagcttgct ggtcgtgaac ggcggcaggg ccgaccgctt cacttctttt ttctccagct
    18541 tttcgacggt gaactccgcg gaccggatgt ccgccgccag tctgcccgct tcttcggcgt
    18601 tggatacctt tgcctttttc ccgccgattt tgatcagctt cgcgtcgaaa ggaggcggcg
    18661 cgccggcctc aaactgcgcc accagattcc agtattcttc cggcacaaac cggctgatct
    18721 cttcttcccg ctcgcagatg atgcgcacgg ccaccgactg cacgcgtccg gcggagagcc
    18781 cccgtttgac tttatcccac aggatcgggc tgatctgata gccgaccaga cggtccagga
    18841 tgcggcgcgt ctgctgcgcc tcgtacttgt cgaaatcgag cggcaacgga tgctgaatgg
    18901 cttccagtac ggtttttttg gtcagatcat tgaaaagaac ccgatagacc ttcttgtttt
    18961 tctttgcatc gacgacgtcg gcgatatgcc aggcgatggc ttctccttcg cggtccgggt
    19021 cgggcgccag gtaaatgttt tcggcgtctt tggccgcttt tttcagatcc gcgatggttt
    19081 tctttttggt ttcgatgacg tgataggtgg gctcaaaatc cttttccaga tcgatgccca
    19141 gcgaactctt cggcaggtct ttgatgtgtc ccatggaggc cagcacatga aaatcctttc
    19201 ccagaaattt cttgatggtt tttacttttg tgggagattc cacgacgatt aatgagtttg
    19261 ccataaacgc tccttgtctg tgggcgcacc atatagatgg tgaaaatgca tttgtaaagc
    19321 ttttattttt tgagcgtgaa caattttccc gggtgctgca aaacccgtcc ctgcagttcc
    19381 aggcgcatca gggcgcccat cgcctcggca gggggaatcc ccgtttcctg aataatatca
    19441 tcggcatggg ttttcccgtc ggcgatggcc ttcaaaattt tccgatcggt cggcaataga
    19501 gtctccacgt ttttttccaa aacggcgcgc tccggcaccc cgtttttttg aggatagggt
    19561 tgctccgatc ctgtttgagt cgccttccgg tccggggaaa ccggctctct ggccgttctt
    19621 tccaactgcg gcagaatctc ttccagaata tcatccacgc tgtcgatcag cttggcgccc
    19681 tgcttgatca ggtggttggc gccgcgcgaa gacgccgagt cgatgggccc gggaattgcg
    19741 aaaacctcgc gcccctgttc catggccagt tttgccgtaa tgagcgagcc gcttttttcc
    19801 ccggcctcca cgaccagaac gccgtaggaa attccgctga taatccggtt gcgcgccggg
    19861 aagtgaaacg gcaggggctg cgtccccggc ggatattcgg agatgacggc gccctgct
//
LOCUS       DAOSRM010000036        19842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2905_length_19842_cov_112.371557, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000036 DAOSRM010000000
VERSION     DAOSRM010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 19842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19842
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2905_length_19842_cov_112.371557"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(105..332)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(369..743)
                     /gene="queD"
                     /locus_tag="PLA58_05115"
     CDS             complement(369..743)
                     /gene="queD"
                     /locus_tag="PLA58_05115"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOR62208.1"
                     /translation="MMEIFKVFTFDAAHWLPRVPEGHKCAKMHGHSFRVEIHIRGKAG
                     SDSGWVTDFAEIAAAFEPLLEQLDHKNLNRIEGLENPTSENLCRWIWVRLKPVLPQLS
                     RIVLQESPESGCIYEGEDEAQP"
     gene            complement(746..1384)
                     /gene="queE"
                     /locus_tag="PLA58_05120"
     CDS             complement(746..1384)
                     /gene="queE"
                     /locus_tag="PLA58_05120"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HOR62209.1"
                     /translation="MYSIKEIFYSIQGEGYHAGRPVVLCRFSGCNLWSGREEERSKSI
                     CKFCDTDFVGTDGINGGVYRSSAELAHRISLTWPVRKNKSVRPFVVCTGGEPLLQLDH
                     ALISDLHEEGFAIAVETNGTIPAPAGIDWLCVSPKAGTPLKQTAGDELKLIFPQAGAE
                     PHRYARLAFRHFFLQPMDGPDREEATRLALAYVLAHPRWRISLQLHKILGVR"
     gene            complement(1532..2254)
                     /gene="queC"
                     /locus_tag="PLA58_05125"
     CDS             complement(1532..2254)
                     /gene="queC"
                     /locus_tag="PLA58_05125"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOR62210.1"
                     /translation="MTDPSVTSKKKAVVLLSGGVDSATTMAIARSMGFNVYALSFRYG
                     QRHMVELEAAARVARGNRVVEHLTVDIDLRRIGGSALTADIAVPKHGSVEELGGNIPM
                     TYVPARNTIFLSYALAWAEVIGSSDIFIGVNALDYSGYPDCRPEYIEAYEHMANLATR
                     AGVEGGQKLRIHAPLIAWSKAQIIKRGMELGVDYSLTHSCYDPDAEGKSCGRCDSCLL
                     RLKGFREAGLADTVVYRLKTED"
     gene            complement(2295..3071)
                     /locus_tag="PLA58_05130"
     CDS             complement(2295..3071)
                     /locus_tag="PLA58_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOR62211.1"
                     /translation="MLIDSHAHLEMKEFDPDRSGVIERARAAGVDCIVTVGTNPDLSR
                     KALSIARRYENIYSTVGIHPHDAAGADDSSLDALKKLAQDPKVVACGEIGLDYFRNLS
                     PREKQTEMFAKQLDLAGELNLPVIIHDREAHDQTMRMVKASGIRSGVFHCFSGDWAMA
                     RQCIDLGFYISVPGVVTFDKSKVLQEVVRQAPIDWMLLETDCPYLAPVPHRGKRNEPS
                     FLVHTARKVAEIKGLAWEDVARTAAVNTRRLFRIAGPVSK"
     gene            complement(3074..3241)
                     /locus_tag="PLA58_05135"
     CDS             complement(3074..3241)
                     /locus_tag="PLA58_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62212.1"
                     /translation="MSKRVNPELLKIDRRIIARKLLIGELSEKDLSSLLKKLPDVSEN
                     AEEIQISSSEK"
     gene            complement(3365..4192)
                     /locus_tag="PLA58_05140"
     CDS             complement(3365..4192)
                     /locus_tag="PLA58_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR62213.1"
                     /translation="MGFWEMFYKNKLALTGCAIVVLLFVVSLLAPWISPCDPGAIDLQ
                     NVLAPPSGEHWFGTDQLGRDVLSRMIWGARISLKVGFVATGLAILIGMILGAVAGYYG
                     GWIDAVIMRFVDVMLCFPAFFLILAVIAFLEPSIWNIMIVIGLTGWMGVTRLVRADFI
                     SLRERDFVRAARAIGANDARIIFLHILPNALASILVAATLGIAGAILTESALSFLGIG
                     VQPPTPSWGNILTAGKDNIDIAWWLSLYPGLAILVTVVGYNLLGEGIRDASDPRLKR"
     gene            complement(4203..5177)
                     /locus_tag="PLA58_05145"
     CDS             complement(4203..5177)
                     /locus_tag="PLA58_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR62214.1"
                     /translation="MFSYLVKRVLFMIPLLLGITIICFFVMRLAPGSPTDLQTQMNPK
                     ASAEMRQRLMSLYELDKPVYVQYASWLTKLARGDLGTSFSSDHRPVADKILERLPITI
                     AINLLSLIIIIAVAVPIGVLSAVHQDSLFDKVMSVIVFIGFAAPTFWLALLLMIFFGI
                     HLGWLPISGLRSLNYEYLSAWEQLVDLAAHLVLPVFISAFGGLAGLSRYMRANMLEVI
                     RQDYILTARAKGLSERQVIYKHALRNALLPAITILGLSIPGLIGGSVIFETIFAIPGM
                     GQLFYMSVMARDYPTVMGILLIGAVLTLVGNLIADVSYAAADPRIRIS"
     gene            complement(5191..5988)
                     /locus_tag="PLA58_05150"
     CDS             complement(5191..5988)
                     /locus_tag="PLA58_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOR62215.1"
                     /translation="MLQIIRKVHVHMPYSLLPQHLPMVLEKKLNPEIYFSHVALQSLD
                     KNQCAGVARALGDAGLKVTFHAPFMDLRPGALDDKIRQASLDRIRQVFDLIPLFQPLK
                     IICHPSFDQRYYVSCDDLWLENSVCFWRQLIPQAEDGGAIIALENVYEREPGILRRLL
                     ETLNSNGVCFCFDTGHFNVFARAPLEDWMEQLGRFIGHLHLHDNFGKFDEHLPVGNAT
                     FPFERFFAALKDLNVRPTITLEAHAQEHLWQSLENLQKTSYLDGLNL"
     gene            6448..6843
                     /gene="rsfS"
                     /locus_tag="PLA58_05155"
     CDS             6448..6843
                     /gene="rsfS"
                     /locus_tag="PLA58_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOR62216.1"
                     /translation="MPFKKNESARRLMLCINAVLQKKAKNIVALNVREISSFTDYMLI
                     CSGTTDRQVQAVSSAVQEYLKKEGIRPLGVEGETNAEWILLDYDDVVISIFQEATRSF
                     YDLENLWDAPRMEIDENATEIKRLNKDMA"
     gene            7050..7568
                     /locus_tag="PLA58_05160"
     CDS             7050..7568
                     /locus_tag="PLA58_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOR62217.1"
                     /translation="MEHPHWFDFARAAVAYEGIILDAIHRFKYGRDLTAGAALAGLLA
                     DFDFAGIDFSAFDAMIPVPLHIRRLRERRFNQSLVLARALGKKHGVAVDFSLLKRRRF
                     TLTQTGLNKSQRDKNISGAFAANHPDKIRGGSFLLVDDVYTTGATLNECAKTLVEAGA
                     KRVAAMTAARVL"
     gene            7657..8142
                     /gene="rfaE2"
                     /locus_tag="PLA58_05165"
     CDS             7657..8142
                     /gene="rfaE2"
                     /locus_tag="PLA58_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOR62218.1"
                     /translation="MDKILDRTALKENLDLLRKEGKKIAFTNGCFDILHVGHVRYLKE
                     ARKTADVLVLALNSDSSVQTIKGPKRPLVPEKERAEVLASLECIDFITIFSEPTPLEL
                     ICLLKPDVLIKGGDWAEEQVVGRKEIRQWGGRVEIIPEVAGKSTTNIVDKVMAVYGLE
                     K"
     gene            8277..8657
                     /gene="dksA"
                     /locus_tag="PLA58_05170"
     CDS             8277..8657
                     /gene="dksA"
                     /locus_tag="PLA58_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HOR62219.1"
                     /translation="MEKQTTLKPEKLEAFRKLLTQKINELLNEAGKTVSEMTSGKENF
                     PDPNDRASLESDRNFELRIRDRERKLIVKMQEAIKRIDDGVYGVCESCGGPISEKRLL
                     ARPVTTECIDCKTKQEKMEKLKGE"
     gene            8762..11170
                     /gene="priA"
                     /locus_tag="PLA58_05175"
     CDS             8762..11170
                     /gene="priA"
                     /locus_tag="PLA58_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOR62220.1"
                     /translation="MFVSVAVNIPADKLFTYEVPAHLQSAVGIGKRVFAPFGLRKRTG
                     FVVEILADCKLKDAKSIVAVLDEEALFDAEDLKFYQWISGYFIYPLGKTLAELIPAGS
                     ERKELRWLIPAPAACDARPTPAQKKLLDILHSYPGGLAFHDLIRKSNLKNAASIARSL
                     QVAGLVHVVEKEKKQLGIRTQKIIRLNEPDADPLKLTPRQQIVADCLKKEGPTRLRAL
                     MEKTATTAAVVKRLLEKGVVCISDEEFIRRAPMESALATPRPDFVLNEEQEKALETLQ
                     QFIASASFHPVLLHGVTGSGKTEVYLCAVEQALKAGGTAIYLVPEISLTPQLISRITG
                     RFDPDKIAVMHSGIAESVRYDQWRQIRRGRIQLVIGARSALFAPLPNLKIIIVDEEHD
                     PSYKQDERLCYNARDLAVVKARFANAVCVLGSATPGVRTFFNARAGKYAHLELAQRVN
                     RQPLPRIDVVDMKMQKGTGGKSPILSDALVAALRSTLNRGEQALLFLNKRGFDTFLVC
                     ADCGYTFSCPNCAVSLKSHPAENVVKCHYCDYSRRAVPLCPRCGGGRILNYGAGTQKL
                     EAELQSLFSKARISRMDSDTTTRRGSQEKILAALERGEIDILVGTQMVAKGHDFPSIT
                     LVGVVSADTSLNMPDFRAAEKTFQMLTQVAGRGGRGRTAGRVIIQTFNPAHYALRHAR
                     GHNYLSFYEEEIEFRKALKYPPFGHIINLRLSSAKQASLDETARELGRDARALCAGLE
                     NIVEIIGPAQSPLAKIRGEHRQQMMIKGRDNRRMHSLAARLLKKHATSTVKITVDVDP
                     ENFM"
     gene            complement(11249..11707)
                     /locus_tag="PLA58_05180"
     CDS             complement(11249..11707)
                     /locus_tag="PLA58_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62221.1"
                     /translation="MAEYIKLLDVLEANIDEIAAHWAADVKKNKRTVHYKDIPDEKLE
                     LQAIKFYSQLRNMIVAPNRLDKAQEFFMHYARTCYEMGVPLHESIYALNMMRRHMWLY
                     AEFQAIFINAIEHNQAIDSVMRVMLLIDYAVYEITQYYQDRIRLEAKQQS"
     gene            complement(11752..12210)
                     /locus_tag="PLA58_05185"
     CDS             complement(11752..12210)
                     /locus_tag="PLA58_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62222.1"
                     /translation="MKTYAIKYVELAEQHAGRMAKRWAMDVQNNVRTKRYKNLDEESI
                     INQGVKFYHNFSKMFAEEKISEATLKYFRTYAQESFAMGIPMDEAVYALILLRRHTWL
                     YAEFQTIFSSGIDQRQALDTLSRTILIFDYAIYEVTKEYQALMKKGKDKK"
     gene            12388..13329
                     /gene="fmt"
                     /locus_tag="PLA58_05190"
     CDS             12388..13329
                     /gene="fmt"
                     /locus_tag="PLA58_05190"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOR62223.1"
                     /translation="MATKPRILFMGTPAFALPALEMLYGSGYPIAAVVTQPDRPAGRG
                     KKETVPPVKQMAQKFGLTVLQPASVKDPSFLEVLGTARPDLVVVAAFGQILPKAVLDF
                     PPLGCLNIHPSLLPAYRGAAPINWSIIRGEKQTGVTIMLMDEGMDSGDILTQEQTPIG
                     RMETYGELHDRLALQGAALLVRTIDQVVSGAARRQKQDPSRATFAPRLNRETGRIRWR
                     HSAGDIVNLIRGLNPSPAAYALLEGRDLKIFTAAAGPGSPQEPPGFVAAATPEGLPVA
                     ASDGYVVLKEVQLAGKKRMPVFDFLRGFTMRPGILLE"
     gene            complement(13330..13728)
                     /locus_tag="PLA58_05195"
     CDS             complement(13330..13728)
                     /locus_tag="PLA58_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOR62224.1"
                     /translation="MFQIVKYETYKDQQIIFKEGTFGDWMYVIEEGAVEISRIVDGKK
                     IVITVLKAGEIIGEVAYISKEGRSATATAVGKTTIGVIDREFFDREFNGISSDFQMIL
                     KSMAQRLRETTHQLMKLQNQVKKQQPPFTV"
     gene            14062..15963
                     /locus_tag="PLA58_05200"
     CDS             14062..15963
                     /locus_tag="PLA58_05200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR62225.1"
                     /translation="MPAGREDHLEKFKKRYPEKFVPEKDLFKCIRRGNSIFIGTACGE
                     PQNLLGSLIRYVENTPHTIYGTQIIHIWTLGVAPYANKKFKINFRHNSFFISDSSRQM
                     VNEGLADYTPTFLSEIPSLFRRGIITIDVALVQLSEPDEEGLMSLGVSVDIVKSAVEQ
                     ARLVIAQVNRHMPRVRGDAMVDIRDVDFILPHDEPILEYTPEPPGEVTEKIGTYVSRI
                     VQDGDTIQVGYGTMPNAILAHLSGKRDLGVHTELLTDGIIDLMKKGVVTNAKKSLDRK
                     KTVASFCMGNRDTYKYLNDNPAFEFRPIDYTNSLFVIGRQTNMTAINSALEIDLTGQS
                     TAESIGGIFYSGVGGQADFMRGSALAPGGKSILALRSTSVDMAHSRIVPCLSAGASAT
                     LTRGDIHYVVTEYGTAYLHGKNIRERAMALISIAHPRFRPWLVEQARERGLIYRDQAF
                     IPGTTGVYPEHLETYRSTRTGLPLFLRPVKISDEGIIKAFLYSLSDKSIYTRFFTNMV
                     HMPHNILQKYVIIDYSKEILMLAIQERQGMEEIVGMGQYIIDTDTHTAAASFLVGDAW
                     QNRGIGIVLLDYLTEIAKRDGLLGFSATVLAENKAMLRLFERMNFQMEKQLSSGVYEL
                     TMGFYPRER"
     gene            16085..17182
                     /locus_tag="PLA58_05205"
     CDS             16085..17182
                     /locus_tag="PLA58_05205"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOR62226.1"
                     /translation="MKKIFFSIASLLVILAGYFLFWPVPIEPVAWNAPAAAGYTGPHA
                     VNTGLSHLNLIDLGAEEGPEHIVLGKDGKLYVSVASGNILRMNPDGSGREVFIRTGGR
                     VLGFDFDAAGNLIAADAIRGLLSVSPQRQITLLTRSVNGSPIRYADAVVVARSGVIYL
                     SDASRRFSPGEWGGPFEASLLDIMEMSSTGRVLAYDPAQKSTHVVAKGFCFANGLALS
                     QDEQHLFVNETGKYRVWKISVNAKELDIAAGGDQAKIVFDNLPGFPDNLMRGQNGKIW
                     LGFAKPRNPDLDQMGSKPFLRKIILRLPRSLWPVPKAYGHVFAFTEDGRVVADLQDPD
                     GAYPETTGVTETADRLYIQSLHAKGLGWLPK"
     gene            complement(17263..17952)
                     /locus_tag="PLA58_05210"
     CDS             complement(17263..17952)
                     /locus_tag="PLA58_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOR62227.1"
                     /translation="MNKIRNILIQSQLFGGLPDEHLAEIERIAVSRHYAKGQVVFHDG
                     DEGIGFYLVVAGIVNVYKLSPDGKEQILHIVREGDTIGAVPVFSGKSFPANARAVTKC
                     HLLFFNRDAFIRLITHQPSLTMNILALLAARLREFTVQVENLSLKEIPGRLASCLLGL
                     AREQGNRDRVELNISKLQLASLLGTGPESLSRALGSMKSRKLIEEKGSTIRLVNRALL
                     EELAEKGKNTR"
     gene            18068..19702
                     /gene="hcp"
                     /locus_tag="PLA58_05215"
                     /gene_synonym="priS"
     CDS             18068..19702
                     /gene="hcp"
                     /locus_tag="PLA58_05215"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOR62228.1"
                     /translation="MFCYQCQETVRNEGCAVRGVCGKTEETSNLQDLLIYLLSGMAIT
                     ADKAKEKGPLNPKYGRFIMEALFATITNANFDSERIIGMIREALRLRGELQAACGAAL
                     PADLHDAAVWHSDHVEEFKKKAAGVGVLSTKNEDVRSLRMLLLYGLKGISAYAQHAAV
                     LDFEDEKIHDFIREALISTTRDLSVEEMVALVLRAGECAVNAMALLDKANTSTYGNPE
                     ISKVNIGVRGNPGILISGHDLKDLDELLQQTEGTGVDVYTHGEMLPANSYPAFKKYAH
                     FVGNYGNAWWKQNEEFESFSGPILMTTNCMIPVKDSYKGRIFTASVVGYPGVKHIPHR
                     VKGGAKDFSEIIALAKKCAPPKELEKGELVTGFGHHQVLALADKVVAAVKSGAIKRFI
                     VMAGCDGRHKSRGYYTDVARELPADTVILTAGCAKYRYNKLTLGDIGGIPRVLDAGQC
                     NDCYSLAVIALKLKEVFGLNDINELPISFDIAWYEQKAVAVLLALLHLGVKGIRLGPT
                     LPAFASPNILKALVENFNIRPIADPKTDVEAMMKGN"
     gene            19707..>19842
                     /locus_tag="PLA58_05220"
     CDS             19707..>19842
                     /locus_tag="PLA58_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR62229.1"
                     /translation="MKKTLRKIIEIDEELCDGCGQCVTGCDEGALQIIDGKAKVVSET
                     F"
BASE COUNT         4679 a         5683 c         5283 g         4197 t
ORIGIN      
        1 ttccgagacg ccccccgctg acaataacaa tgttctgaat catggatcat ccaaaataaa
       61 aaaaaccata cccttttgcc tgcaaaggac atggctcttg atgaatgaat agatgccgcc
      121 actccctacg ccggcattat ccgggtcagg ttcaaagggt atattctcag ccgcgacttg
      181 aaatgagagc tttgaataac ggccattttt gtcatttcga ccgaagggag aaatcttgaa
      241 caaacggccg tcatgaagat ttctcgtcgc tcacgctcct cgaaatgaca tttttcaaag
      301 ctctcgaaat cgcggcaccc cgcggggaaa aagtagtaca ttcaccgtta aaaagcaacg
      361 gattaaattc acggctgcgc ttcatcttcc ccttcgtaaa tgcacccgga ttccggactt
      421 tcctgaagga caatccggga caactgcggc aggacgggtt tcagacgcac ccagatccag
      481 cggcaaagat tttccgaggt gggattttcc agaccctcga tccggtttaa atttttatga
      541 tccagctgct ccaggagcgg ttcaaaagcg gcggcgatct cggcaaaatc cgtgacccaa
      601 ccgctgtccg aaccggcttt cccccggatg tgaatttcga cacgaaaaga atggccgtgc
      661 atcttcgcgc atttgtggcc ctcgggcacg cgggggagcc agtgcgccgc gtcgaatgta
      721 aagactttaa agatttccat catgcttatc gaacccccag gattttatgc aactgcaggc
      781 tgatgcgcca ccgggggtgc gccaaaacat atgccagcgc cagcctggtg gcttcttccc
      841 tgtccggacc atccatcggc tgcagaaaaa aatgccggaa cgccaaacgc gcgtaccggt
      901 gcggttcggc gcccgcctgc ggaaaaatca gttttagttc gtccccggcc gtttgcttca
      961 gtggagtgcc ggctttcgga ctgacgcaga gccagtcgat cccggcgggc gcggggatcg
     1021 tgccgttggt ttccacggca atggcaaagc cttcctcatg caaatcgctg atcagcgcgt
     1081 gatccagctg caacagaggc tcgccgcccg tgcagaccac aaaaggacga acggacttat
     1141 tttttctgac cggccaggtc agggagatcc tgtgcgccag ttcggcagag gatcgataga
     1201 cgcctccgtt gatgccgtcc gtcccgacaa agtcggtgtc gcaaaatttg caaatcgatt
     1261 tggaacgttc ttcttccctg cccgaccaca gattgcaccc cgaaaaacgg caaagcacaa
     1321 cgggacggcc cgcgtggtat ccctcaccct gaatggaata gaagatttct ttgatggaat
     1381 acataagtcc tttagtctga aggctgaaga ccgaaggcaa aaagccttca aaccttgtca
     1441 gatttaactc tgtatcttcg tttttataac tctgtacctg ttacgccacc tcgcacaccc
     1501 tttagggcat caggtggctt tccttcagtc ttcagtcttc agtcttcagt ctataaacaa
     1561 cagtatccgc aagccccgcc tctcgaaacc ctttcagccg aagcaggcag gaatcacatc
     1621 ggccgcagga cttgccttcc gcatccggat cgtagcaact gtgcgtcagc gaataatcga
     1681 cgcccagctc catgcctctt ttgataattt gcgctttgct ccaggcaatc agcggcgcgt
     1741 gaatccgcaa tttttgtccg ccttccacac ccgcccgcgt cgccaggttg gccatgtgtt
     1801 catatgcctc gatatattca ggccggcagt ccggatagcc gctgtagtcc agagcattga
     1861 cgccgatgaa aatatcggaa gacccgatca cctccgccca ggccagggcg taagacagga
     1921 agatggtgtt tctggccgga acataggtca tggggatgtt tccccccagt tcctcaaccg
     1981 acccatgttt aggaacggcg atgtccgccg tcagagccga cccgccgatg cgccgcagat
     2041 cgatatcgac cgtcaggtgc tcaaccacac ggtttccacg ggcaacgcgc gcggccgctt
     2101 ccagttcaac catgtgccgt tgaccgtacc ggaagctcaa ggcatatacg ttaaagccca
     2161 tgctccgcgc tatcgccatc gtagtggcgg aatccacgcc gccggagagc agcaccaccg
     2221 cctttttctt tgatgtcacg gatggatcgg tcataacaag agcatttagc agattttgaa
     2281 gaatttctcc cgcgctattt agaaaccggg cctgcgatac ggaaaagtcg acgggtgttg
     2341 accgccgcgg ttctcgccac gtcctcccag gcaaggcctt tgatttcagc gactttccgc
     2401 gcggtatgga caagaaaaga cggctcgttt ctcttaccgc gatggggaac gggcgcaaga
     2461 tagggacagt ccgtttccag aagcatccaa tcgatcggcg cctgccggac aacctcctgc
     2521 agcactttgg acttgtcaaa ggtgacaacg ccgggcacgg aaatataaaa accgagatcg
     2581 atgcactgcc tggccatggc ccagtcgccg gaaaaacagt gaaagacccc cgagcggatg
     2641 ccggaggctt tcaccattcg catcgtctga tcatgcgctt ctcggtcatg gatgatcacc
     2701 ggcaggttca gctcaccggc caaatccagc tgtttggcaa acatttccgt ctgtttttca
     2761 cgtggggaaa gattgcgaaa ataatccagg ccgatctcgc cgcaggcgac caccttcggg
     2821 tcctgcgcca gcttcttcag ggcgtccagc gacgaatcat cggctccggc cgcatcatgg
     2881 ggatggatgc cgacggttga ataaatgttt tcataccggc gggcaatgga cagcgccttg
     2941 cggctcaagt ccggattggt tcccaccgtc acgatacagt caacgcccgc cgcccgggcg
     3001 cgttcaatca cgccggatcg atcgggatcg aactccttca tctccaggtg ggcatgggaa
     3061 tcgatcagca taactacttt tcgctggacg aaatctgaat ttcttcggcg ttctccgaaa
     3121 catcgggaag cttttttaaa aggctggaca gatctttttc cgacaactct ccgatcagca
     3181 gcttgcgcgc aatgattctg cggtcaattt tcaacaattc cgggtttact cttttggaca
     3241 tttcgatcct cctgaacagt tcggcaacgt tacacggtca taccatccgc gaccgttgcg
     3301 gtctcctatt acattttgca gacataatca agcccccggc attcgggaca acggaccggg
     3361 ccggtcatct tttcaatcgc ggatcggacg cgtcacggat cccttcgccc agcagattat
     3421 agccgaccac cgtgacgaga atggccagcc ccgggtacag cgacaaccac caggcaatat
     3481 cgatattgtc tttgcccgcc gtcagaatat tcccccaact gggcgtcggc ggctgcacgc
     3541 cgatgcccag aaagctcagc gccgactccg tcaggattgc cccggcaatg cccagggtgg
     3601 cggccaccag tatggatgcc agcgcgttgg gcagaatgtg cagaaaaatg atccttgcgt
     3661 catttgcgcc gatggcccgg gccgctcgaa cgaaatcccg ctcgcgaagc gaaatgaaat
     3721 ccgcccgcac cagacgggtc acccccatcc agcccgtcag cccgatcacg atcataatgt
     3781 tccagatgga gggctccagg aaggcaatca ccgccaggat cagaaagaaa gcgggaaagc
     3841 agagcatgac atccacaaag cgcatgatca ccgcgtcgat ccagccgccg taataacccg
     3901 cgacggcgcc cagaatcatg ccgatgagaa tggccagccc cgtggcgaca aagccgactt
     3961 tcagggaaat acgcgcgccc cagatcatcc gggaaagaac atcgcggccc aattggtccg
     4021 tgccgaacca gtgctcgccc gacggaggcg ccagaacatt ctgcagatcg atcgcccccg
     4081 gatcgcaggg cgaaatccag ggggccagca gtgaaacaac aaacagcaga accacaatgg
     4141 cgcagccggt cagcgccagt ttgttcttat aaaacatttc ccagaagccc atcacaaagc
     4201 cctcaggaga tcctgatgcg gggatccgcc gccgcgtagg acacatccgc aatcagattc
     4261 cccaccagcg tcaaaaccgc gccaatcaac aggatcccca tcacggtcgg ataatcgcgc
     4321 gccatcaccg acatatagaa caactgcccc atgcccggaa tcgcgaaaat cgtttcaaag
     4381 atgacgctgc cgccgatgag gccggggatg gacaatccca gaatggtaat cgccggcagg
     4441 agcgcgttgc gcagggcatg cttgtaaatc acctgccgct cgcttaatcc cttggcgcgc
     4501 gccgtcagaa tataatcctg ccggattact tccagcatat tggcccgcat gtagcgggaa
     4561 agccccgcca ggccgccgaa agccgaaata aacaccggca aaacgagatg cgccgccaga
     4621 tcgaccaact gttcccaggc gctcaggtat tcataattga gggaccgcaa accggaaatc
     4681 ggcagccagc ccagatggat gccgaaaaaa atcatcaaaa gcagggccag ccagaaggtc
     4741 ggcgcggcaa aaccgataaa aacgatcacg gacatcactt tgtcgaaaag cgagtcctga
     4801 tggaccgccg acaaaacgcc gatcggcacg gccaccgcaa tgataatgat cagcgacagc
     4861 agattaatgg caatcgtaat cggcaaacgc tccagaatct tgtcggccac cggccggtgg
     4921 tcggatgaaa aggatgttcc cagatcgccg cgggccagct tcgtgagcca ggatgcgtat
     4981 tgaacataaa cgggcttatc cagttcatag aggctcatca ggcgctggcg catttccgcc
     5041 gacgccttcg gattcatctg cgtctgcagg tccgtggggg agccgggcgc caggcgcatc
     5101 acaaaaaagc agatgatcgt gatgcccagc agaagcggga tcatgaaaag gactctcttg
     5161 accagatacg aaaacatcct tctctcctga tcagagattc agaccgtcca gatacgacgt
     5221 cttttgcaaa ttttccaggg actgccagag gtgctcctgg gcgtgcgcct ccagcgtgat
     5281 cgtcggtctg acattcagat ctttcagtgc ggcaaaaaac cgttcaaagg gaaacgtggc
     5341 gtttcccacc ggcaaatgct catcgaattt tccaaaatta tcatggagat gcaaatgacc
     5401 gatgaaacga cccaactgtt ccatccagtc ctccaggggc gcccgggcaa aaacattgaa
     5461 gtgacccgtg tcgaagcaaa agcaaacgcc attggaattc agcgtttcca gcaggcggcg
     5521 caggatgccg ggctcccttt cgtaaacgtt ttccagtgcg atgattgccc ctccgtcttc
     5581 ggcctggggg atgagctgcc gccagaaaca aacgctgttt tccagccaca gatcatcgca
     5641 ggacacataa tagcgctgat caaaggacgg atggcaaata attttcagtg gctgaaaaag
     5701 ggggatcaga tcaaacacct gccggatccg gtccaggctg gcctggcgaa tcttgtcatc
     5761 cagcgcaccg ggccgcaagt ccataaaagg ggcatgaaac gtcactttta atccggcatc
     5821 tccgagcgcc cgggcaacgc cggcgcactg atttttatcc agcgactgca gcgcaacgtg
     5881 gctgaaatag atttccggat tgagcttctt ctccagaacc atcggcagat gctgaggcag
     5941 cagggaataa ggcatgtgga catgaacctt ccggatgatc tgaagcattt gcgattcctt
     6001 tctctatcga cagggatcgt tgatttacca tagtcatttt tttttcacaa cccgaatgaa
     6061 attgttgtac gtaccttcag gcgcgccttt gaagcgaacc ggataacctg ccttcacgcg
     6121 agcctctttt aaaaagctgt attgtcaagt ttttgcattt gtgacatata gaaacagact
     6181 gaagaccgaa ggttgaaggc tgaaggttta cggggtgcgc cttcggcatt taacatttct
     6241 gagagagctt tgaaaaatgt catttcgagg agcgtgagcg acgagaaatc ttaaggatgg
     6301 ccgtttgttc aagatttctc ccttcggtcg aaatgacaaa aacggccgtt attcaaatct
     6361 ctctgaatga cggcgcgata cgcgcttcat ctctttgcct tcagtcttcg gacttcagcc
     6421 tataaaccta tgcatcagga ggatcctttg cccttcaaaa agaatgaatc cgcccggcgg
     6481 ctgatgctgt gcatcaacgc ggttttacag aaaaaggcta aaaatatcgt agccttgaat
     6541 gttcgggaga tttcttcctt tacggactac atgctcatct gctctgggac aaccgaccgg
     6601 caggttcagg ccgtttcctc cgcggttcag gaatatctga aaaaagaggg catccgtccg
     6661 ctgggcgtgg aaggcgagac caatgccgaa tggatcctgc tggattatga cgatgtggtg
     6721 atctccatct ttcaggaagc cacccgctca ttctacgatc tggaaaacct gtgggacgcg
     6781 ccgcggatgg agattgatga aaacgcaacg gaaatcaaga gattaaacaa ggatatggct
     6841 tgatttttcg tgagtttctc tctcaaattt ccgatgcttt tttcccgaga atctgtctgg
     6901 cctgctcgtg cgttcttccc accggccgcc cggagttctt ctgcgccgat tgtttttgcc
     6961 ggatcacctt tcttgacgga agcctctgtc cggtctgcgg ggtaacattt cccgactcgt
     7021 ccgccggaaa ccacctgtgc ggttcatgca tggaacatcc tcactggttc gattttgccc
     7081 gcgccgctgt tgcctatgag ggaatcatcc tcgatgcaat ccaccggttc aaatacggac
     7141 gggacctgac ggcaggcgcg gcgctggcag gtcttctggc cgattttgat tttgccggaa
     7201 tcgatttcag cgcttttgac gcgatgattc ccgtgccttt gcatatcaga cggctgcggg
     7261 aaagacggtt taaccaatcg ctggttctgg cgcgcgcttt gggcaaaaaa catggcgtcg
     7321 ccgtcgattt ttccttgcta aaaaggcgaa gatttacttt aacgcaaaca ggattgaaca
     7381 aaagccagcg ggataaaaac atttccggcg cttttgccgc aaatcatccg gacaagatcc
     7441 gcggcgggag tttcctgctg gtggatgatg tttacacgac aggggccacc ctcaacgaat
     7501 gcgccaaaac gctggttgag gcaggcgcga aacgggtcgc ggcgatgacg gcagccagag
     7561 tcctttaatc tctgatggac tacccgcggc aaccggcgcc ctgaagagat caaccttata
     7621 gaaatggttt tccgcctggt caggaaagga gtcatcatgg acaagattct ggacagaacc
     7681 gccttaaagg aaaatctgga cctgctgcgc aaagagggaa aaaagatcgc ttttaccaac
     7741 ggctgctttg atattcttca cgtaggtcat gtccgttacc tgaaggaagc cagaaaaacg
     7801 gctgatgttc tggttctggc gctcaacagc gactcttccg tgcagaccat caaggggccc
     7861 aaaaggccgc tggtccccga aaaggagaga gccgaagtcc ttgcctcttt ggaatgcatt
     7921 gattttataa cgatattttc agaaccgacc cctctggagc tgatttgcct gctgaagccc
     7981 gatgttttga ttaaaggcgg cgactgggcc gaagagcaag tcgtcggccg caaagaaatc
     8041 cgtcagtggg gtggacgggt tgagatcatt ccggaagttg cgggtaagtc cacgaccaac
     8101 atcgtggaca aggtcatggc ggtctatgga ctggaaaaat gaaggcgaaa cgggcgggtg
     8161 ggtatgccgg atggcggttt ttttcggggt ttttatgcat gatttttttg ctttacaaac
     8221 cacctgccat taggtaaaag gatcgtccaa atattacagg taaaggagtg gcggccttgg
     8281 aaaaacaaac gactttaaag ccggaaaagc tcgaagcctt ccgcaaactg ctgactcaaa
     8341 aaatcaatga actgctcaac gaggcgggga aaaccgtctc ggaaatgacc agcggcaagg
     8401 aaaatttccc ggatcccaac gaccgcgcct ctctggaatc cgaccgcaat tttgagctgc
     8461 gcatccgcga ccgcgagcgc aaactcatcg tcaaaatgca ggaagccatc aaaagaatcg
     8521 acgacggcgt ttacggcgtc tgtgaatcct gcggcggccc gatttccgaa aaaagactgc
     8581 tggccagacc cgtcacgacc gaatgcatcg actgcaaaac caagcaggaa aaaatggaaa
     8641 agctcaaagg cgaataaaga ccggaccgga atccaaaaag ccccgccaag tcggggcttt
     8701 ttttatggcc ggttttttca tccgatattt tctttttccg gcaaaagtgg taacaaggcc
     8761 catgttcgtt agcgtcgccg tcaacattcc ggccgataaa ctcttcacct atgaagttcc
     8821 ggcccatctt cagagcgccg tcgggatcgg caaacgcgtt tttgcgccgt tcggcctcag
     8881 aaaacgaacc ggatttgttg ttgaaatatt agccgactgc aagctcaaag atgccaaatc
     8941 aatcgtcgcc gtcctcgacg aagaggcgct gttcgacgcg gaggacctga aattttatca
     9001 gtggatttcc ggctatttca tctatccgct gggaaaaaca ctggccgagc tgattcccgc
     9061 gggatcggaa agaaaagagc ttcgctggtt gattcccgcg cccgccgctt gtgacgcgag
     9121 gccgaccccc gcgcagaaaa agcttctgga catcctgcat tcatatcccg gcggcctggc
     9181 tttccatgat ctgatccgaa aatccaatct gaaaaacgcg gcttcgatcg cccgcagcct
     9241 ccaggtggcc ggcctggttc atgtggtgga aaaggaaaaa aagcagctgg gcatccgtac
     9301 gcaaaagatc atccggctca atgaaccgga tgccgatccg ctgaagctga cgccccggca
     9361 gcagatcgtc gccgattgcc tgaaaaaaga agggccgacc cggctccgcg cgctgatgga
     9421 gaaaaccgca acgaccgccg ccgtcgtcaa aaggcttctg gagaaaggcg tcgtctgcat
     9481 cagcgacgag gaatttatcc gcagagcccc gatggaatcc gccctcgcca ccccccgtcc
     9541 ggattttgtg ctcaatgaag agcaagagaa agccctggaa actcttcagc aatttatcgc
     9601 aagcgcgtcg tttcatccgg tcctgctgca cggcgtgacg ggcagcggca aaacggaagt
     9661 gtatctttgc gccgtcgagc aggcgctgaa ggcgggaggc acggccattt atctggtccc
     9721 ggaaatttcc ctcacgccgc agttgatttc ccggatcacc gggcggtttg acccggacaa
     9781 aatcgccgtg atgcacagcg gcatcgccga gagcgtccgc tacgaccagt ggcgccagat
     9841 ccggaggggc cggattcagc tggtgatcgg cgcgcgctcg gcgctgtttg ctccgctgcc
     9901 gaacctgaaa atcatcattg tggatgagga gcacgacccc tcgtacaagc aggacgaacg
     9961 gctctgctac aacgcccgcg acctggccgt cgtcaaagcc cgtttcgcaa acgccgtctg
    10021 cgtgctcggt tcagcgacgc cgggcgtgcg caccttcttc aatgcccgcg ccggaaaata
    10081 cgcgcacctg gaattggccc aaagggtcaa ccgccagccg ctgccgcgca tcgacgtcgt
    10141 ggacatgaaa atgcaaaagg gaaccggcgg caaatcgccg attctctccg atgcgctggt
    10201 cgcggcgctt cggagcaccc tgaaccgcgg ggagcaggcg cttctttttc tcaacaagcg
    10261 cggattcgat acatttctgg tctgcgccga ctgcggctac accttttcct gccccaactg
    10321 cgcggtttcc ctgaaaagcc atccggcgga aaatgtcgta aaatgccact actgcgatta
    10381 cagccgcagg gccgtgccgc tctgcccccg gtgcggcggc ggccggatcc tcaactacgg
    10441 cgcgggcacg cagaaactgg aggccgaact gcaaagcctg ttttcgaagg cgcgcatcag
    10501 ccgcatggat tccgacacga cgacgcgccg cgggtcgcag gaaaagattc tggccgcgct
    10561 ggaacgcggc gaaatcgata tcctggtggg gacgcagatg gtcgccaagg ggcacgattt
    10621 tccgtccatc accctggtgg gcgttgtctc cgccgataca tcgctgaata tgccggactt
    10681 ccgcgccgcg gaaaaaacgt ttcaaatgct gacgcaggtg gcggggcggg gcggaagagg
    10741 acggacggcg gggcgcgtca tcattcagac cttcaacccc gcgcattacg cgctgcgtca
    10801 cgcgcgcggc cacaactatt tatcttttta cgaagaggaa atcgagtttc ggaaagcgct
    10861 gaagtacccg cccttcggac acatcatcaa cctccgcctg tcctcggcaa agcaggcctc
    10921 ccttgacgaa acggcgcggg agctgggacg cgacgcccgg gcgctttgcg ccgggcttga
    10981 aaacatcgtg gagatcatcg gtccggccca atcgccgctg gcgaaaatcc ggggagagca
    11041 ccgccagcag atgatgatca agggaaggga caaccggcgg atgcattccc tggccgcccg
    11101 gcttctgaag aagcacgcca cgagcacggt aaaaatcacg gtggatgtgg acccggaaaa
    11161 tttcatgtga agggaacaca caaaaacggc agcgaaacat ccgctgccgt cggttgagtc
    11221 cgttcaaatc agtctgcccc gcaggacgct aactctgctg tttggcctcc agccgaatgc
    11281 gatcctgata gtattgtgtg atttcataca ccgcgtaatc gataagcagc ataacccgca
    11341 tcacggagtc gatggcctga ttatgctcga tggcgttgat aaaaatcgcc tgaaactccg
    11401 cgtagagcca catatggcgg cgcatcatgt tcagggcgta gatggattca tgcagtggaa
    11461 cacccatttc gtagcaggtt ctcgcgtagt gcatgaagaa ttcctgcgct ttgtccagac
    11521 ggttgggggc aacgatcata ttccgcagct ggctgtagaa tttaatggcc tgcagttcaa
    11581 gcttctcatc gggaatgtcc ttataatgaa ccgttcgttt atttttcttg acatcagcgg
    11641 cccagtgcgc cgcgatttca tcaatattgg cttccagcac atccagcagc ttaatatatt
    11701 ctgccataat cctttctcct ctcttgcatc ctttacgggt aagggggcgt gttatttttt
    11761 gtcttttccc tttttcatca atgcctgata ttccttggtc acttcataaa tcgcgtagtc
    11821 aaaaatcagg atcgttcggc tcagggtatc cagggcttgc cgctgatcga tgccggagct
    11881 gaagatggtc tggaactccg cataaagcca ggtatgtctt cttaaaagaa tcaatgcata
    11941 gaccgcctca tccatgggaa tccccatggc aaaactttcc tgggcatacg tgcgaaaata
    12001 tttcagggtg gcttcgctga ttttttcctc ggcaaacatt ttactgaaat tgtggtagaa
    12061 cttgacgccc tgattaatga tgctttcttc atccaggttt ttgtatcttt tcgtccggac
    12121 attgttttgg acgtccattg cccatctttt ggccattctt cccgcatgct gctcggccaa
    12181 ctccacatac ttaatggcgt atgtcttcat gcttcatctc ctttcttgaa agatccactg
    12241 gtcatgagaa ttgtttcgtt gaatttaata taaagcatga aattaaaatt gacaacagga
    12301 aaatgccata aaaccgccgg tcacgggtaa ttcttgacac aaaccccaca attccatata
    12361 ggatcacgaa aacaacaagg acccgacatg gcgacaaaac ctcgcattct cttcatgggc
    12421 actccggctt ttgctctgcc ggcgctggaa atgctttacg gcagcggcta cccgatcgcc
    12481 gcggtcgtca cccagccgga ccgccctgcg ggaaggggaa aaaaggaaac cgttccgccc
    12541 gtgaagcaaa tggcgcaaaa attcggcctc acggttttgc agccggcaag cgtgaaagac
    12601 ccgtcttttc tggaagtcct gggcacagcc cgcccggatc tggtggttgt ggcggctttc
    12661 ggacagattc tgcccaaagc cgtccttgac ttcccgccgc tcggttgcct gaacattcac
    12721 ccttcccttc tgcccgccta ccggggagcg gcgccgatca actggagcat cattcggggt
    12781 gaaaaacaaa ccggcgtaac cattatgctg atggatgaag gaatggattc cggcgatatc
    12841 ctgacccagg agcaaacgcc catcggccga atggagactt acggtgaact gcatgaccgg
    12901 ctcgctcttc agggcgcggc gctgctcgtt cgaacgattg atcaggtcgt ctccggcgcc
    12961 gcccgaagac aaaagcagga tccctcccgc gcaaccttcg ccccccgcct gaaccgggaa
    13021 accggcagaa ttcgctggcg tcattccgca ggcgacattg ttaatctgat ccgcggcctg
    13081 aatccgtcgc ccgcggccta tgcgctcctg gaggggcggg acctgaaaat ttttaccgcc
    13141 gccgcaggcc cgggttcgcc gcaggaaccg cccggttttg tcgccgcggc cactcccgaa
    13201 ggattgcccg tggcggcctc cgacggctat gttgttttga aagaggttca gctggccgga
    13261 aagaaaagaa tgcctgtttt tgacttcctg cgcggcttta caatgaggcc gggaatcctg
    13321 ctggaatgat caaacagtaa aggggggttg ctgttttttg acctggtttt gaagtttcat
    13381 cagttgatgg gtcgtttctc tcaggcgctg ggccatggat ttcaggatca tctggaaatc
    13441 actggagatt ccattgaact ctctgtcaaa aaattcccgg tcgatcactc cgatcgttgt
    13501 tttgcccact gcggttgccg tcgcggaacg cccctctttg gaaatatagg ccacctcccc
    13561 gatgatttct cccgctttca ggacggtgat aacgatcttt tttccatcaa cgatccggga
    13621 gatttccacg gccccctctt caatgacata catccagtcg ccaaatgttc cctctttaaa
    13681 gataatctgc tgatccttgt atgtttcata ttttacaatc tggaacatca agcgcctcct
    13741 tttgcctttc tcataccgga ctcattgacc ggtttttttc taacatgatt ttcacttcca
    13801 tacaacagac aatcccgcag gcccacttcc tgaacagcgc ctcaaatccg gccgccgctt
    13861 ttaccgggaa acgagtcaag ttcttcttga catacaaaac gggactttct atagttcccg
    13921 ggcaaaggaa agctctcccg taatttcatc gtgttttggg caaatcggca aaggccgatc
    13981 ccgggcgcga aacgaacggc cgaacgcaag gccggccggt cccgcaggac ctggagacgg
    14041 gaaagcaacg ggggaacggc catgcctgca gggcgggaag accatcttga aaaattcaaa
    14101 aagcgctacc cggaaaaatt cgtccctgaa aaagaccttt tcaaatgcat ccgccgcggc
    14161 aattccatct tcatcggcac ggcgtgcggc gaaccgcaga acctgctcgg ctcgctcatc
    14221 cgctacgtag aaaacacgcc ccacaccatt tacggaacgc agatcattca catctggacc
    14281 cttggcgtgg cgccctacgc caacaaaaag ttcaaaatca actttcgcca taattcgttt
    14341 tttatcagcg acagttcccg acaaatggtc aatgaagggc tggccgacta cacgccgacc
    14401 ttcctttcgg aaatcccctc cctctttcgc cgcggaatca tcaccatcga cgtcgcactg
    14461 gtgcagctct cagagccgga cgaagaaggc ctgatgagcc tgggcgtgag cgtggacatc
    14521 gtcaaatccg ccgtggagca ggcccggctg gtgatcgcgc aggtcaaccg gcacatgccg
    14581 cgcgttcgcg gcgatgccat ggtggatatc cgcgacgtgg atttcatcct cccccacgac
    14641 gagcccatcc tggaatacac gcccgagcca cccggcgaag tgaccgaaaa gatcggaacc
    14701 tatgtctcgc gcatcgtgca ggacggcgac accattcagg tgggatacgg aaccatgccc
    14761 aacgccattc tggcccatct ttcgggaaag cgggatttgg gcgtgcacac ggagcttctc
    14821 accgacggca tcatcgatct catgaaaaaa ggcgtcgtga ccaacgcgaa gaaatccctg
    14881 gaccgcaaaa aaaccgtcgc gtcgttctgc atgggaaacc gcgacactta caaatacctg
    14941 aatgacaatc cggcgtttga gttcaggccg atcgactata ccaacagcct ctttgtcatc
    15001 ggacggcaaa ccaacatgac cgccatcaac tccgcgctgg aaatcgatct cacgggacag
    15061 tctaccgcgg aatcgatcgg cggaatcttt tacagcggcg tgggaggaca ggcggatttc
    15121 atgcggggct cagcgcttgc gccgggcggc aagtccattc tggccctgcg ctccacctcg
    15181 gtcgacatgg cccactcgcg catcgtcccc tgcctttccg ccggcgcgtc ggccacgctc
    15241 acccgcggcg acatccatta tgtggtcacg gaatacggaa cggcctacct gcacggaaaa
    15301 aacatcaggg aacgcgccat ggctctcatt tccatcgcgc acccgagatt ccgcccctgg
    15361 ttggtggaac aggcgcgcga gcggggcctc atctaccgcg accaggcgtt tatccccgga
    15421 acaacgggcg tgtatcccga gcatctggaa acctaccgct ccacgcggac cggccttccg
    15481 cttttccttc gccccgtcaa aatcagcgac gagggaatca tcaaggcctt tctctactcg
    15541 ctgtcggaca agagcatcta cacgcgcttt ttcaccaaca tggtccacat gcctcataac
    15601 atccttcaaa agtacgtgat catcgattac tcgaaagaaa tactgatgct cgccatccag
    15661 gagcggcagg gcatggaaga gattgtgggc atgggtcagt acatcatcga cacggacacc
    15721 cacacggccg cggcctcctt cctggtgggc gacgcctggc agaaccgcgg catcggcatc
    15781 gtgctcctcg attacctcac cgaaatcgca aaaagggacg ggctgctggg attttccgcc
    15841 acggtgctgg cggaaaacaa agccatgctc cgccttttcg aacgaatgaa ttttcaaatg
    15901 gaaaagcagc tctcgtcagg cgtatacgag ttgaccatgg gattctaccc gagagaacgc
    15961 tgaacatttt gattcccgtt tcttttgcaa agacattgcc aagactccga gcggcgcggc
    16021 agtttcgttt taaccctatt gatcattcaa ccggtcacac aagtattatc ggaaggaggt
    16081 cctgatgaaa aaaatatttt tttcgattgc ctcgcttttg gtgatcctcg cggggtattt
    16141 ccttttctgg cccgtgccga tcgagccggt cgcctggaat gcgccggcgg cggccggata
    16201 taccggaccg cacgcggtca acaccggatt gtcccatctg aacctcatcg acctcggcgc
    16261 cgaagagggc cccgaacaca tcgtcctcgg aaaggacgga aaactgtacg tctccgtagc
    16321 aagcggaaac attctgcgca tgaatccgga cggcagcgga cgggaggttt tcatccgcac
    16381 cggcggtcgc gtgctgggat ttgattttga cgcagcgggg aatctgattg ccgcggacgc
    16441 aatccgggga ctgctgtccg tcagccctca aagacagatc acccttctga cccgttcggt
    16501 caacggcagc ccgatccgct atgccgacgc ggtggtggtg gcccggtccg gcgtgatcta
    16561 tctgagcgat gcgtcaaggc gcttttcccc cggggaatgg ggcggaccgt ttgaagccag
    16621 cctcctcgac atcatggaga tgtcctccac cggacgagtc ctggcctatg atccggcgca
    16681 aaaatccacc cacgttgtcg ccaaaggatt ttgctttgcc aacggcctgg ccttaagcca
    16741 ggacgagcag caccttttcg tgaacgagac gggaaaatac cgggtctgga aaatctccgt
    16801 aaatgcaaag gaactggaca tcgcggcggg cggcgatcag gccaagattg tttttgacaa
    16861 tcttcccggc tttcccgaca atctgatgcg ggggcagaac ggaaaaatat ggctgggctt
    16921 tgccaaaccg cgtaatcccg atctggatca aatggggagc aaaccatttc ttcgaaagat
    16981 tatcctgcgt ctgccgcggt ccctgtggcc ggttcccaaa gcgtacgggc atgtgttcgc
    17041 cttcacggaa gacggccggg tcgtggccga cctgcaggat ccggacggcg cgtatccgga
    17101 aaccaccggc gtgacggaaa ccgccgaccg gctttatatt cagagcctgc atgccaaagg
    17161 gctcggctgg ctgcccaaat agcccgacga cattcaaagc cggcatcgga aaaaacgcga
    17221 aaaaaacttc ctccggggca catccgccgg agggggagcg ttttaccgtg tgttttttcc
    17281 tttttcggca agttcttcca gcagcgcccg gttcaccagc cggatcgtgg accctttttc
    17341 ctcgatcagc ttcctgcttt tcatgctgcc cagggcccgg gaaagcgatt ccggtccggt
    17401 gcccagcagg ctggccaact gcagcttgga aatgttgagt tcgacccgat cccggtttcc
    17461 ctgctcccgc gcgaggccca gaagacagga cgccagacgg ccggggattt ccttgagcga
    17521 cagattttcc acctggacgg taaactcccg cagtcgtgcg gccagcaagg ccaggatatt
    17581 catcgtgagg gacggctgat gcgtaatcag acgaataaac gcatctctgt tgaaaaacag
    17641 aagatggcat ttggtcactg cgcgggcgtt ggcgggaaaa gacttcccgg aaaaaaccgg
    17701 aaccgcgccg atggtgtcgc cctcccggac gatgtgcagg atctgctcct ttccatcagg
    17761 ggaaagcttg taaacattga cgatccccgc gaccaccaga taaaagccga tgccttcatc
    17821 gccgtcatgg aaaacgacct gccccttggc gtagtgccgg gaaaccgcta ttctttcgat
    17881 ttccgcaaga tgctcatccg gaagtcctcc aaaaagctgg ctttggatca gaatattccg
    17941 aattttgttc atccgttgcg cctcccggta aaaaattttt tctttactta acataaatca
    18001 aggatgtccg gaacgatttt cagtatcttt gcggaaaatc atcgtgaatc ttcacaggag
    18061 gtgtcccatg ttttgttatc agtgccagga aaccgtcagg aatgaaggct gcgccgtcag
    18121 aggcgtctgc ggcaaaaccg aggaaacatc caatctgcag gacctgctga tttatctatt
    18181 gagcggcatg gccatcactg ccgacaaggc taaagaaaaa gggccgctga atcccaaata
    18241 cggacgcttc atcatggaag cgctttttgc cacgatcacc aatgccaact tcgacagcga
    18301 acggatcatc ggaatgatcc gggaggcgct ccggcttcgc ggcgaacttc aggcagcctg
    18361 cggcgcggca ttgcccgccg acctgcatga cgctgccgtg tggcattccg accatgtgga
    18421 ggaatttaaa aagaaagcgg ccggcgtcgg cgttctttcc acaaaaaatg aggatgtgcg
    18481 ctcgctgcgt atgcttctgc tctatggcct caagggaatt tccgcatacg cccagcacgc
    18541 cgccgtgctg gacttcgagg acgaaaaaat tcatgatttc atccgggaag cgctgatctc
    18601 caccaccaga gatctgagcg tggaggagat ggtcgcgctg gtcctgcggg cgggcgaatg
    18661 cgccgtcaac gccatggccc tgctggacaa ggccaacacc tcgacgtacg gcaatcccga
    18721 aatctccaaa gtcaacatcg gcgtgcgggg caatccgggc attctgattt ccggccacga
    18781 cctcaaggat ctcgacgaac tgctgcagca gacggaagga accggcgtgg atgtttacac
    18841 acacggcgag atgctgcccg ccaacagcta ccccgctttc aagaaatacg ctcacttcgt
    18901 tggaaattac ggaaacgcct ggtggaaaca gaacgaggag ttcgaatcct tcagcggccc
    18961 catcctcatg acgaccaact gtatgattcc ggtgaaggat tcctacaaag gcaggatctt
    19021 caccgccagc gttgtcggtt atcccggagt gaagcatatt ccgcacagag taaaaggcgg
    19081 ggcaaaagac ttctctgaaa tcatcgcact ggcaaaaaaa tgcgctccgc cgaaagagct
    19141 ggaaaagggc gagctggtga cgggtttcgg gcaccatcag gtgctggccc tggccgacaa
    19201 ggttgtggct gcggtaaaat ccggagccat caagcgcttt atcgtcatgg ccggatgcga
    19261 cggtcggcat aaatcccgcg gctattacac cgatgtggcc cgggaactcc ccgcggacac
    19321 ggtcatcctc accgccggct gcgccaagta ccgctacaac aagcttacgc tgggcgacat
    19381 cggcgggatc cccagggtgc tggatgccgg gcagtgcaac gactgctatt cgctggcggt
    19441 gatcgcgttg aagctcaaag aggttttcgg tttgaacgac atcaacgaac tgccgatttc
    19501 ctttgacatc gcctggtatg agcagaaagc cgtggcggtg ctgctggccc tgctgcacct
    19561 gggcgtcaag ggaatccgcc tcgggccgac gctgcccgcc tttgcctcgc cgaacatcct
    19621 caaggcgctg gtggagaatt tcaacatcag accgattgcg gatccgaaga cggatgttga
    19681 agccatgatg aaagggaatt aacgttatga aaaagacact gcgaaaaatc attgaaatcg
    19741 acgaagagct gtgcgacggg tgcggccaat gcgtgaccgg ctgcgatgag ggcgccctgc
    19801 aaatcatcga cggcaaggcc aaagtcgttt cggaaacatt ct
//
LOCUS       DAOSRM010000037        19798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2912_length_19798_cov_106.090260, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000037 DAOSRM010000000
VERSION     DAOSRM010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 19798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19798
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2912_length_19798_cov_106.090260"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLA58_05225"
     CDS             complement(<1..874)
                     /locus_tag="PLA58_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62230.1"
                     /translation="MTMNRPKTVRSDQTKFKDLLARPEVRARWEKVRRYFFLRESTYD
                     MTNRCNIRCDGCYYYEGDKPFAAENCSPEDWRSLMRAEKARGITYVVLAGAEPSLAPD
                     LLAVCYAEMPLGCIATNGLKKIPATVGYKIHISVWGNDATSFKTRSAKNLLKKQIENY
                     RNDPRAVFVYTFTRDNIGEARDVAAELVAAGCKMTFNVFSSPVGYAGPLRHNAESLLK
                     TRETMMALLSRYPAQVLFSAYNAVAHTSDRGLNDLYRCSYPRCNPSQDIGLGRSFRQY
                     RTDLNWDRSAACCVP"
     gene            complement(896..1168)
                     /locus_tag="PLA58_05230"
     CDS             complement(896..1168)
                     /locus_tag="PLA58_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOR62231.1"
                     /translation="MPENSALKAIVRRVAEIIIDELKLEDVTPDTFNPEMDLVDELGI
                     DSMDLATIALVLQDEYKIVIDEDDYPKLKTVRLICEYIEEKIAPRH"
     gene            complement(1183..1557)
                     /locus_tag="PLA58_05235"
     CDS             complement(1183..1557)
                     /locus_tag="PLA58_05235"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62232.1"
                     /translation="MTDRWKLLKDVQWTALDGIEAKADVPENSVWFKGHFPGEPILPG
                     IALISTVYEAVLKDASHRGESLKISSLKRVRFTGPVRPGEKMALILAREVQNGEILFH
                     FKVTVRGNMVCSGLMTVAKAQE"
     gene            complement(1688..2494)
                     /locus_tag="PLA58_05240"
     CDS             complement(1688..2494)
                     /locus_tag="PLA58_05240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOR62233.1"
                     /translation="MEDGKKRLSPVLITGGTVLISAAAVFFVHPLLSAAVLSLYVGVC
                     VASCFFPQTNFLGPVIHRGRTGKNVVALTFDDGPFEATTEKILALLDRHDAKATFFVS
                     GVNAARCPNLIRELIRRGHTIGNHSMSHDPFLMLKGSRTLQREVVEAGEVLRKMGIDA
                     LAFRPPVGIVNPKLFPILKAEGLFCVTFSCRARDAGNRRIRNLSGRILQKIKGDDIVL
                     LHDRPRQGSDDPDVLWNEMEKILAGIRARGLKIVPLAELAGRDIMRLKSL"
     gene            complement(2648..4000)
                     /locus_tag="PLA58_05245"
     CDS             complement(2648..4000)
                     /locus_tag="PLA58_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HOR62234.1"
                     /translation="MKKVLLISANRMKKPYPVYPLGLDYVAGALKPRYQTKILDMNFC
                     PSLDMLGVQVREYEPDYIGFSIRNIDNTDLMSPRGFLSDYQDMIGIIRMQSKAPLILG
                     GSGYTVFPLEFFRALGADFGVAGEGERLSLLLDTLEQKGNAASIPGVITSETSSVRYL
                     PWKGDLHRVFDPDAEYTKFYISYGGMLNLQTKRGCPFRCSYCTYPHIEGGKMRLLDPR
                     EVAHQARRLQDAGAKYIFVTDSAFNASYEHSLQVARAFKDRGVTVPWGGFFAPTVPPE
                     GYFQQLADAGLSHVEFGTESLCDTMLSNFRKPFAAADVFEAHRQAEKAGLHIAHYFLL
                     GGPDENRETLRETLEGVDRLNRAVFFFFCGIRIYPHTALYETALREGQIQASQNLMDP
                     VFYRSPFLSGPEIIRIVETHAAGRINWLIGAGEGKATRILPRLYERGHTGPLWEYLIA
                     "
     gene            complement(4059..4331)
                     /locus_tag="PLA58_05250"
     CDS             complement(4059..4331)
                     /locus_tag="PLA58_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOR62235.1"
                     /translation="MSTVEETIKDLKGKIIATLGLLDVTPEDIKDDDPLVGGETGIDS
                     IDVLELVMMIEKDYGVKIDSKELGAKVFASVRTLATFILQVRASRA"
     gene            complement(4473..4883)
                     /locus_tag="PLA58_05255"
     CDS             complement(4473..4883)
                     /locus_tag="PLA58_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62236.1"
                     /translation="MSILKKTALYFARKEYCRSAILERADLSAIKEKPTPKIFVGLGL
                     IAFSYAIGLPAVVAMGVIAVWIKEPLVLVVGGPLIYAVSTIVFIIGIKMAGKKYFQVF
                     SRWLTRVVLEKILGKDHPSFAGTDSDNCPGGGSA"
     gene            5047..5478
                     /locus_tag="PLA58_05260"
                     /pseudo
     CDS             5047..5478
                     /locus_tag="PLA58_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            5572..5742
                     /locus_tag="PLA58_05265"
     CDS             5572..5742
                     /locus_tag="PLA58_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOR62237.1"
                     /translation="MQAAARYVCSICGYVYDPAEGDPENGIARGTRFEDIPDAWTCPV
                     CGAEKKMFEVDK"
     gene            5767..7890
                     /locus_tag="PLA58_05270"
     CDS             5767..7890
                     /locus_tag="PLA58_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62238.1"
                     /translation="MTDTVKQSSLDVLVAMNTVVKNIRFYPLSSALVMNGVENLYQVF
                     LNYFKAYPSFILSESERTLLIGDEPLTQKDHEKPYVVSTLEILLKFNIKSIALHRGLE
                     KRELVTLLAHLAKDPEKILDEGGLQEIIEKNHLSHIRLDEKIFVAKGKNQKIVSSLEL
                     TDDQILQYLSESHPELQDDLQKILEMTDGPEWLLKFFEENLSKLAAQENALPDVQLAE
                     KLTGIVEQIDQVSDRLDPNTQQKIRDGIDRFVGSIRPGTAREILLQNQNSSTSGTLMR
                     RLVGKLRQDALGTASEGPEGAADFNQEEPQEADSLPQIQENIERLLGDSAKGVLDEAV
                     MSSLVRSVEALAAQKERAPLETIMNRFLANMFAEDAKVRDQAARALIEIVENLPDKEK
                     QTVLRGIAGKLLDWVKLETLATQSYRKICDFLRDHVNHLIWQDDFHAAIPMLDFFNDV
                     RTGVIEKNDTIHEISVDFIDACASDERIAILFNIFKEGEPDQKRDAGEMLIRLGDAAM
                     NQLLDLLKEQRDSNERVRIMRLIISAGRRAIPPVRDRIGKDQPWFYLRNIAYILSHVG
                     DESSAEALQPLLLHENIKVRLEALKGINRTGKSQRCALLLSVLPQADHKFMINIVDVL
                     GSAKCAEAEPALLILLENNPIKDKDLRANLQERICAALGSIGLPNSIPTLSKIVETKS
                     FLGISRYPENVKAAAARALASIKKR"
     gene            complement(7992..8507)
                     /locus_tag="PLA58_05275"
     CDS             complement(7992..8507)
                     /locus_tag="PLA58_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62239.1"
                     /translation="MAGKEYPRVKQLRTFPIVLLAAILLYAPAGIAQEYTLRDLYIEA
                     VKNSEKIKYAEENLYIAKMGRMKAWSVLMPRVTAFGAYNRYSEAKFTVSGALSQPNEA
                     DNWVVRADESFSLSIRKLDALKISGQLVVKSDFDLENTKSDFILSVATAIMTRSKRKK
                     RSKSRRPMWSG"
     gene            complement(8522..9856)
                     /locus_tag="PLA58_05280"
     CDS             complement(8522..9856)
                     /locus_tag="PLA58_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR62240.1"
                     /translation="MTTKCQGLRIVIVAVFLLFSPSAVSAQALTLEESIAIALKNSYV
                     LDIAKEGVKGAQAQRKEALTGFLPKFSTTYSYTRLNEEPNFYFPGFPLAGIPAGRMVT
                     GTIDNYNWGIEARQPLFAGGGILANYQAFKIAEDTALVEQTAKSLDVIQDVKIAYFNV
                     LRTQRLTQTARQQVEMLAAHCDVAENFYRVGLIPKNDMLQAEVELANGRQTLVKAKNG
                     VESTKARLNTILKRDVRTAVEVVDMLDCQPLRQSFEECLEIARQHRPELKMSALKIEQ
                     AGKMVRAAQSEYFPAVSLVGNYGRFGDSTSLSGTQYKDRESWQVMAVANWNFWEWGKT
                     KYRVDAGKAGENQAQDQSRELSDQIALEIKNAFLALQEAESQIAVSKKVIEQAEENFR
                     IAEARYKERVARSTEVLDAQTLLTKAKAEYANALADYNIAYARLQRAMGVTH"
     gene            complement(9946..10416)
                     /locus_tag="PLA58_05285"
     CDS             complement(9946..10416)
                     /locus_tag="PLA58_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62241.1"
                     /translation="MEKKKIPVAMSGGAVSSVAALLLREAGYAVAGATMLLGVHRLGD
                     EAGRFTNESVADAQSVCGRLGIPHRIFDFADLMEQRGIGQFVRECQNGQTPNPCVDCN
                     RHLKFGALPRHPGRRQGEGCFHTGPGSDYAGAIRRFYTADEVLGGGIIEEVVRV"
     gene            10643..11887
                     /locus_tag="PLA58_05290"
     CDS             10643..11887
                     /locus_tag="PLA58_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR62242.1"
                     /translation="MEIHFLLFVLLLAATAIISFAYAIWRGRNDQQILRSILQSSPIP
                     TFVISTDRKILYWNRALEGLSGIRAEEVLGTDQQWRAFYSSPRSCMADLIISNKIDHS
                     YSLYAGKVTKSKVLKYAYEVTEFFPDLGEKGKWFRITATSIRDSSGKTFGAMETLEDI
                     TDQKTAEEELLKRTKLESLGTFADGVAKDFDSLLTAILRNVFLAKISASEEDKILENG
                     LAVAERAGLQAKELAHRLITFAQGGYPVKKIERIGDLIRETVQKTLAGSKVTCAPVIA
                     ADLWPVEVDALQIKQVIENMVTNAKEAMPEGGPMEVIAENTTTGTNIRTLGEGNYVRI
                     VIRDKGVGIPRENLSKIFDPYFTTKEKKERGGIGLGLAICDSIIKYHNGLISMESTVG
                     VGTTFFIYLAAMPNGSPKSNVS"
     gene            complement(11884..12078)
                     /locus_tag="PLA58_05295"
     CDS             complement(11884..12078)
                     /locus_tag="PLA58_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62243.1"
                     /translation="MTRSERALLFCLAEEIILHLRNRLAEIENLHPRESALGIATFQE
                     RLRHIEELLDGVKKEHERSN"
     gene            complement(12094..12891)
                     /locus_tag="PLA58_05300"
     CDS             complement(12094..12891)
                     /locus_tag="PLA58_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOR62244.1"
                     /translation="MSTGSDKRICVICAWRENCTKRYRVKTNALFDVNCPDYTRDIKL
                     RDKDVDKLVEDQVMRWQRDKKEKPGHVITLSSETGAGGGFISRILATELKMNIVGSEL
                     IHKVAESAHMSEKVIKSLDEKSISFFDSLISSFFESRHIWPNEYLRHLSMVMHTNAEH
                     GNVIIIGRGGSFILKDKAFRVRIIAPEEARVEKVMRDRHMSKAEALEYVRKYDEYQVG
                     FIKNYFKEDINDPKHYDMMVNTDKVSIESAAESIKKAFLLRKSMQQE"
     gene            complement(12919..13809)
                     /locus_tag="PLA58_05305"
     CDS             complement(12919..13809)
                     /locus_tag="PLA58_05305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOR62245.1"
                     /translation="MQTTESLKRRMKSAGDLLSVVKTMKALAAVSIRQYQRAVDSLRD
                     YNRTVEMGLQIVLKERMGATMKRKNVPIKRLGVIVFGSDQGLCGQLNEQISTFALDHI
                     KSTGVKKENRKVLSVGARVADYVEDAGQTVDELLSTPGSTAGITPLVQEIIMIIDEWH
                     FRGNVDHFFLFYNEYLTGATYHPHMLQLLPVDEAWLKQIARKKWESKSLPIFRMDGDK
                     IFSSLIREYLFVSLYRAFAESLASENASRLASMQNAEKNIEEQLLDLHVQFHRTRQMT
                     ITEELLDIVAGFEALQEEAS"
     gene            complement(13796..15364)
                     /locus_tag="PLA58_05310"
     CDS             complement(13796..15364)
                     /locus_tag="PLA58_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternate F1F0 ATPase, F1 subunit alpha"
                     /protein_id="HOR62246.1"
                     /translation="MNQEVSQVESQELKTFLDDTFDTMEKVLEEQNPELRQYEIGTVQ
                     FVGRDIARVSGLPHIRAEELVRFSGNYMGLVFNIDPKEIGVILLDPSENIQVGSEVQR
                     TKRVLDVPVGDGLLGRVVDPLGRPLDGLGEVNTVMRLPVERPAPAVMDRAPVTVPLQT
                     GIKVIDALIPVGRGQRELILGDRMTGKTAIAVDTIINQKETGIISIYCATGKKSADVA
                     KVIAELRDHGVMKSCIVVVATGEDTPGLQFIAPYAATSMGEYFVEQGRDVLIVYDDLT
                     SHARAYREVSLLLRRPPGREAFPGDIFYIHSRLLERSTHMRPELGGGSLTSLPITETE
                     AQDMSSYIPTNLISITDGQIYLSPVLFSKGILPAVDVGKSVSRVGGKTQLPAYRSVAG
                     DLRLSYSQFEELESFSRFGTRLDDATRRTLERGWRVREILKQGQFKPLRASEQIASLL
                     SVTGGALDLVPTEKIREVEARLLETVNEQLPELCTRIEEGKKMDMTDRDSIMNKIKPT
                     IAPFEEIEEAHANN"
     gene            complement(15425..16195)
                     /locus_tag="PLA58_05315"
     CDS             complement(15425..16195)
                     /locus_tag="PLA58_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62247.1"
                     /translation="MHIDWFVFLAQIFNFLLLMYLLKRFLYGRIIKAMDDREAKIAAR
                     FAEADELKTKAEEEALLYEKRNQSLNEKKETMLNEATLAAEAKRKELMEKVRVEVDAV
                     KARWQDMLIREQDAFFYDLRQRAAKQLYATARKALSDLADADLEERIVEEFLRRVQSL
                     DEEKSTQLRKAISGGGNRVTIQSAFGIPAPRQTQIEEVLKKQITNGFTIRYLQEPDIV
                     SGIEMRVNGHKIAWSLNEYLETLVESLTETLQKEAHAA"
     gene            complement(16207..16473)
                     /locus_tag="PLA58_05320"
     CDS             complement(16207..16473)
                     /locus_tag="PLA58_05320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOR62248.1"
                     /translation="MDNVSLVAMASIIGAGLAMGIGSIGPGLGMGQAIAQALAAIAQQ
                     PDERNSLTRTLFVGLAMIESIAIYCFVISMILIFANPFWGFIIK"
     gene            complement(16501..17229)
                     /locus_tag="PLA58_05325"
     CDS             complement(16501..17229)
                     /locus_tag="PLA58_05325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOR62249.1"
                     /translation="MEIVQLSQISPDQVVLWSWSFITINVTILYTWLVMAILVGGSIL
                     VTRNLSSEMNVSRWQHFLEVVISVVRGEISEMTKKGADSYVPLIGTLFLFICTSNVLA
                     VVPGYVAPTSSITTTAALATCVFIAVPYYGISRNGILHYVKEYFQPTFIFFPFHVMGE
                     LSRTLALTVRLFGNIMSHEKVIGILLAVTPFLFPVVMQILGLLIGVIQAYIFAILSLV
                     YIASALSAEEEAHHTEAKKEGSAA"
     gene            complement(17266..17568)
                     /locus_tag="PLA58_05330"
     CDS             complement(17266..17568)
                     /locus_tag="PLA58_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOR62250.1"
                     /translation="MTLNAMGMLVLMFAAGMALGALYFVGLWQTVKRLPQTESRARLL
                     LVSLLARLSVLLAVFYFLLQDGRWERLAAALIGFVVMRKILTVRLCPQKTAEAIQE"
     gene            complement(17570..17899)
                     /locus_tag="PLA58_05335"
     CDS             complement(17570..17899)
                     /locus_tag="PLA58_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOR62251.1"
                     /translation="MVEIRRTTNRRRQMADHFAEKVAQREALRIKGLKHKNETVWFGL
                     GMFGIVGWAVAIPTLIGVALGLWIDRTWPSQYSWALMGLVAGVIVGCINAAYWVRKIR
                     SRIIEEE"
     gene            complement(17976..18356)
                     /locus_tag="PLA58_05340"
     CDS             complement(17976..18356)
                     /locus_tag="PLA58_05340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOR62252.1"
                     /translation="MLLKILLPAEIMMEREVKKIVAEAENGSFCLMPNHIDFVATLAP
                     GIFSYEPTEGGHELLAMDVGTLVKKGADVLVSTRNAVKAAELGKLKQVVVEQYDALDE
                     REKMVRSAAAKLEASLIRRFVELK"
     gene            complement(18346..19731)
                     /gene="atpD"
                     /locus_tag="PLA58_05345"
     CDS             complement(18346..19731)
                     /gene="atpD"
                     /locus_tag="PLA58_05345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOR62253.1"
                     /translation="MAEQTNQGRVLSVRSSVVDIHFPVAPPIRNVLTAGEKGEVVIEV
                     FTQLDNNTIRGVALTPTQGLARGAVVTDTGKPFEVPVGSQLLGRVFNVFGNTIDKKGN
                     VEGCEARSIHREPIPLRDQSTVSEIFETGIKAIDVLAPLERGGKAGLFGGAGVGKTVL
                     ITEMIHNTVSGHEGMSIFCGIGERCREGEELYREMEESGVLGNTVMVFGQMNEPPGAR
                     FRVGHAALTMAEYFRDDAKQDVLLMIDNIFRFIQAGMEVSGLLGQLPSRLGYQPTLAT
                     ELAELEERICNTKTGAITSIQAVYVPADDFTDPSAVHTFGHLSATIALSRKRASEGLY
                     PAIDLLQSGSKMLMPNIAGERHYKIAQEIRKTLAVYEDLKDIIAMLGISELSREDQRT
                     VFRARRLERFFTQPFFVTEQFTGTAGKMVSREETLNGCERILNDEFAEYPERALYMIG
                     PIEEAKIENVA"
BASE COUNT         4494 a         5831 c         5078 g         4395 t
ORIGIN      
        1 ccggcacgca gcaggccgcc gaccggtccc agttcaaatc cgtccggtac tggcgaaaag
       61 agcgccccaa tccaatgtcc tgcgagggat tgcagcgggg atacgaacag cggtaaagat
      121 cattgagccc gcgatcgctc gtatgcgcaa cggcgttata ggcggaaaag agcacctgcg
      181 ccggatatcg tgacaaaagc gccatcatcg tttccctggt tttcaaaagc gattccgcat
      241 tgtggcgcag gggaccggca tatccgaccg gagaggaaaa aacattaaat gtcatcttgc
      301 agccggcggc gaccagctcc gcggcaacat cccgggcttc gccgatgttg tcgcgcgtaa
      361 acgtatacac aaaaaccgcg cgtggatcgt ttcggtaatt ttcaatctgc tttttcagga
      421 gatttttggc gctgcgcgtt ttgaagctcg tcgcgtcgtt gccccagacg gaaatatgaa
      481 tcttgtagcc gaccgttgcc ggtatttttt ttaaaccatt ggtggcaatg caacccagcg
      541 gcatttcggc atagcacacg gcaagcagat ccggcgccag cgagggctcc gcacccgcca
      601 gcactacata ggtgataccg cgggcctttt ccgcccgcat caggcttcgc cagtcttcgg
      661 gggaacaatt ctccgcggca aagggcttgt ccccttcgta ataatagcag ccgtcgcagc
      721 ggatgttgca gcggttggtc atgtcgtagg tggattcccg cagaaagaaa tacctgcgca
      781 ccttttccca tcgcgcccgg acctccgggc gggccagcag gtctttaaat ttcgtctggt
      841 ctgatcgaac ggttttcgga cggttcatgg tcatggagat ctttgtcttt tgccttcaat
      901 gccggggagc gatcttttct tcaatatatt cacaaatcaa ccggacggtt ttcaattttg
      961 gataatcgtc ttcgtcgatg acaattttgt attcatcctg caacaccagc gcgatcgttg
     1021 ccagatccat gctgtcgatg cccagttcat ccaccaggtc catctccgga ttgaaggtgt
     1081 ccggcgtcac gtcttccagt ttaagctcgt cgataatgat ttcggccaca cgccgcacaa
     1141 tggccttcag tgcgctgttt tcaggcattc cagcctcctt tttcattcct gtgcttttgc
     1201 caccgtcatc agtccgctgc agaccatgtt tccccgaacc gtcactttaa agtgaaacag
     1261 gatttcgccg ttctgcacct cccgggcaag gatcagagcc attttttctc ccggacggac
     1321 cggcccggta aaccttactc ttttcaaaga agagattttg agggactctc cccggtggga
     1381 tgcatccttc aaaaccgctt catagacggt gctgatgaga gcaattcccg gcaggatcgg
     1441 ttcccccgga aaatgacctt taaaccaaac ggaattttcc ggcacgtcgg ccttggcttc
     1501 gatgccgtcc agcgcagtcc actggacatc tttgagcaat ttccatcgat cggtcaaaat
     1561 cctttccttt ccccgttgat tcgtttacgc ccgaataaag gatgagttct atatcactta
     1621 ttatttattc aatcaaaaat tttttcagca ggcgaataca aaaagaattt caatcaaaga
     1681 caagccgtta caacgatttc agcctcatga tgtcccggcc cgccagctcg gcgagcggca
     1741 caatcttcag ccctctggcc ctgatgcccg ccagaatttt ttccatttcg ttccataaaa
     1801 cgtcaggatc atcagagccc tgccgcggcc ggtcatgcaa aaggacgatg tcgtctcctt
     1861 ttattttttg aagaatcctg cccgaaagat tccgaatgcg ccggttcccc gcgtcccgcg
     1921 cccggcaact gaaggtgacg caaaacaatc cctccgcctt cagaatggga aaaagcttcg
     1981 gattgacaat gccgaccggc gggcggaagg ccagggcatc gattcccatt tttcgcaaaa
     2041 cctccccggc ttccacaact tcgcgctgca gcgtgcggct ccccttcagc atcaggaaag
     2101 gatcatgact catggaatga ttgccgatcg tgtgaccgcg ccggatcaac tcgcgaatca
     2161 gatttggaca gcgcgcggcg ttgacaccgg acacgaagaa agtggctttc gcgtcgtgac
     2221 ggtccagcag ggcaagaatc ttttccgttg tggcttcgaa agggccgtca tcaaaagtga
     2281 gggcgacaac attttttccc gttcgtcccc ggtggatgac cggccccaga aaattggttt
     2341 gagggaaaaa gcaggatgcc acgcacacac ccacatacaa agacaacacc gcggccgaaa
     2401 gcagtggatg aacaaaaaaa accgcagccg cggagatcaa aaccgttccc cctgtaatca
     2461 ggacggggct tagccgtttt ttcccgtctt ccatgaaaag tctctccggg taagccgttt
     2521 agaaacagcg tgacaacggc ttgaatcacc tgaccactca accacctaac cgcctaaagg
     2581 tggcgcgagg tcacgcgatg tggtacgagg tcaaccagcc cttccccgcg aaaaagccgg
     2641 accgatgcta tgcgatgaga tattcccaca acggacccgt atgcccgcgc tcgtagagtc
     2701 tgggcaggat gcgcgtcgcc ttgccctccc cagccccgat cagccagttg atccttccgg
     2761 cggcatgcgt ttcaacgatc cggatgattt cagggccgga cagaaaaggc gagcggtaaa
     2821 aaaccggatc catcagattc tgcgaggcct gaatctgtcc ttcgcgcaaa gccgtctcgt
     2881 acagcgccgt gtgtgggtaa atgcgaatac cgcagaaaaa gaaaaaaacc gcccggttca
     2941 gccgatcgac accctccagg gtctcccgca gggtctcccg gttttcgtcg gggccaccca
     3001 gcaaaaaata gtgggcgatg tggaggccgg ccttttcagc ctgccggtgg gcctcaaaga
     3061 catcggcggc ggcaaaaggc ttcctgaaat tcgacagcat cgtgtcgcaa agcgactccg
     3121 tgccgaattc cacgtgcgaa agaccggcat ccgccagttg ttgaaaataa ccttccgggg
     3181 gaacggtggg agcaaaaaat cctccccagg gaacggtgac gccccggtcc ttgaaagccc
     3241 gggcaacctg aaggctgtgt tcgtaactgg cgttgaatgc ggaatcggtc acaaaaatat
     3301 atttggcgcc ggcgtcctgc agccgacggg cctgatgggc gacctcccgc gggtccagca
     3361 gccgcatttt gccgccttca atgtggggat aggtgcagta actgcatcga aacgggcatc
     3421 cccgtttggt ctgcaaattc agcatgccgc catacgagat ataaaatttt gtgtattcgg
     3481 catcgggatc aaagacccgg tgcagatctc ccttccaggg gagatagcga acggacgaag
     3541 tttcagaggt aatcaccccc ggaatagagg ccgcgttccc cttctgttcc agcgtatcga
     3601 ggagcaacga aagcctctcc ccttctccgg caacgccgaa atccgcgccg agcgcccgga
     3661 aaaattcgag gggaaacacc gtgtagccgc tcccgcccag aatgagcggc gccttcgatt
     3721 gcatccggat aatgccgatc atgtcctgat agtcggaaag gaaaccgcgg gggctcatca
     3781 ggtccgtgtt gtcgatattg cgtattgaaa atccgatgta gtccggttcg tactcccgga
     3841 cctgcacccc cagcatatcc agagacggac aaaaattcat atccagaatt ttcgtttgat
     3901 aacggggctt cagggcgccc gctacatagt caagccccag cggatacacc ggatagggct
     3961 tcttcatcct gttggcggaa atcagcaata ctttcttcat gaaaccattc tgctttggat
     4021 cgttggaaga tcagggccgt ttccagcccc ggcaggaatc aggctctgga ggctctgacc
     4081 tggagaatga aggtcgccag tgtgcggacc gaagcaaata ccttggcgcc cagttccttg
     4141 ctgtcaattt taacgccgta atccttttca atcatcatca ccagctccag cacatcgatg
     4201 gaatcaatgc ccgtctcgcc gccgaccaac ggatcatcat cctttatgtc ctcgggggtt
     4261 acgtccagca accccagcgt tgcaatgatt ttgcccttca ggtctttgat ggtttcttct
     4321 accgtgctca tgcgtcacct caatcgatta atgaaaactt ctcccggcat cccgccgggc
     4381 atcctaaatc cgcctcgaag ggatcggatg aagtcccccc ccgctgaagc cgaaagcagc
     4441 ggcccttgcg cccgctgccg gagctttgtg cctcaagcgc ttccacctcc cgggcagttg
     4501 tccgaatccg tccctgcaaa agacgggtga tccttcccca gaatcttttc cagcacaacc
     4561 ctggtcaacc agcgggaaaa cacctggaaa tatttttttc ccgccatctt gattccgata
     4621 atgaacacaa tggtggaaac ggcatatatc agcggtcctc cgacgaccag taccagcggc
     4681 tccttgatcc agaccgcgat gacgcccatg gccacaaccg ccggcaaacc gatggcataa
     4741 ctgaacgcga tcaggccgag gccgacgaaa attttaggcg tcggtttttc cttgatggcc
     4801 gacagatcgg ctctttccaa aatcgccgat ctgcaatatt ctttgcgggc aaaataaagg
     4861 gccgtttttt tgagaatgct caaaaccagt ctcaaccacg cagcttaatt aaaaacgtgg
     4921 ggatcatatc tgcaatcggg aaagttttgc aatagttttt tggccttcag aaatttcttg
     4981 gcataaaagg gcaaattcgg ctataagaac gtattattaa ctcatccgga aaagaggaaa
     5041 gaaaatatga acagagcagc ccttcaccag atcagttacg gcgtctatat tgtcacctca
     5101 ggcaaagacg gcaccttcaa cggtcagatc gccaactcca ttgtccaggt tacctccaaa
     5161 cccgccaccc tggccatctg catcaataaa gaaaactaca cacacgcctt gatacggaaa
     5221 agcggaaaat ttgtcgtttc catcctgtcg gaggccgctc cgatgacctt catcggactt
     5281 ttcggcttta aaagcggacg cgacgtcgat aagttaaaag acgtcaaaag caggatcggc
     5341 gccaccggcg tgccgatcgt tctggattat tccctgggat ttgccgaggc cgaagtggaa
     5401 ggcgaactgg acggcggaac gcacacgatt ttcctgggca aagttgtcga ggcggatctg
     5461 gtgggggaag gcacaccaat gacatacgcc tattatcaga acgtcaaggg cggcaaatcc
     5521 cccaaatccg ctccgacatt tgatgcggac gcggccgttg ccaaacccaa aattcaggcc
     5581 gccgcccgct atgtgtgcag catctgcggt tatgtctatg accccgccga gggcgatcca
     5641 gaaaacggca tcgctcgggg cacccgattc gaagacattc ccgacgcctg gacctgcccg
     5701 gtttgcggcg cggagaaaaa aatgtttgaa gtggataagt aagttttcag gaaggagaaa
     5761 acagccttga cagacacggt aaagcaatcc agcctggatg ttcttgtcgc catgaatacg
     5821 gtggtcaaga acattcgctt ctatccgttg agcagcgccc tcgtcatgaa cggagtggaa
     5881 aacctttacc aggtttttct gaactatttc aaggcttatc cgtcgtttat tctgtcggaa
     5941 tccgaaagaa ccctgttgat cggcgatgag cccctgacgc aaaaagatca cgaaaaaccc
     6001 tacgttgtct ccaccctgga gattctcctg aagttcaaca tcaaaagcat tgcgctgcac
     6061 agagggctgg aaaagaggga attagtcact ctgctggctc acctcgcgaa agatccggaa
     6121 aaaatcctgg acgagggcgg cctgcaggaa atcatcgaaa aaaatcattt gtcccatatc
     6181 cgcctggatg aaaaaatatt cgtcgccaaa ggaaaaaatc aaaaaatcgt ctccagcctg
     6241 gagctcaccg acgaccagat tcttcagtat ttgagcgaat cgcaccccga attgcaggat
     6301 gacctgcaaa agattctgga aatgacggac ggtccggagt ggctcttgaa gttctttgag
     6361 gaaaaccttt ccaaactggc cgcgcaagaa aacgccctgc ccgacgttca actcgcggaa
     6421 aagctgaccg gcatcgtgga acagatcgat caggtttcgg accggctgga cccgaatacg
     6481 cagcaaaaga tccgtgacgg aatcgaccgg ttcgtcggca gcatcagacc aggcacggcc
     6541 agagaaattc tcttacaaaa ccaaaacagc tccacaagcg gcacgctgat gcggcgcctg
     6601 gtcggcaaac tccggcagga cgccttggga acggcatccg aaggcccgga gggcgcggct
     6661 gatttcaatc aggaagaacc ccaggaagcg gacagcctgc cgcagattca ggaaaacatc
     6721 gaacggctgc tcggcgacag cgcaaaaggc gtcctggatg aagcggtcat gtcgtccctg
     6781 gtccgctcag tggaagccct ggccgcgcaa aaagaacggg cacccctcga aaccatcatg
     6841 aaccgcttcc tggcaaacat gtttgccgaa gacgccaaag tgagggacca ggcggcgcgc
     6901 gcgctgatcg aaattgttga aaatctgccg gataaagaaa agcaaaccgt ccttcgggga
     6961 atcgccggca aactgctgga ctgggtcaag ctggaaacgc tggcaaccca gtcctatcgg
     7021 aagatctgcg actttctcag ggaccatgtg aatcatctga tctggcagga tgactttcac
     7081 gcggccattc caatgctgga ctttttcaac gacgtccgga cgggcgtgat cgaaaaaaac
     7141 gacacgatcc acgagatctc cgttgacttt atcgacgcct gcgcctctga tgagcgaatt
     7201 gccatcttgt tcaatatttt caaggagggc gaaccggatc aaaagcgcga tgcgggagag
     7261 atgctgatcc ggcttggcga cgccgccatg aatcagcttc tggaccttct caaggagcaa
     7321 cgggacagca atgaacgcgt tcgcatcatg cggctgatca tcagcgccgg ccgaagggcc
     7381 ataccgcccg tccgcgaccg gatcggcaag gatcagccct ggttttacct gcggaacatc
     7441 gcctatatcc tgagccacgt cggagatgaa tccagcgccg aagccctgca gccgctgctg
     7501 ctccacgaaa acatcaaggt gaggctggag gccctgaaag gcatcaatcg caccggaaaa
     7561 agccagcgct gcgcgctgct cctttccgtc ctgccgcagg cggatcataa attcatgatc
     7621 aatatcgttg acgtgctcgg cagcgcgaaa tgcgccgagg cggagcctgc gctgctcatc
     7681 ctgctggaaa acaatccgat caaagacaaa gacctgcggg caaatctgca ggaaaggatc
     7741 tgcgccgctc tcggcagcat cggactgccc aactccatcc ccaccctgtc caaaatcgtc
     7801 gaaaccaaat cctttttggg aatcagccgg tacccggaaa atgtgaaggc cgccgccgcc
     7861 agagcgctgg cgtccattaa aaaaaggtag cgcctccccg gttcaaacgc actcctccaa
     7921 aggaaaggtg aacgccctca gccccgcgcg tgggttttcc gccttgaatg tccgaaccag
     7981 acggtactgc gtcagccgct ccacattggc cgccgcgatt tcgagcgctt ttttcgcttt
     8041 gagcgtgtca taatagccgt cgctacggaa aggataaaat ccgatttggt gttctccagg
     8101 tcaaaatcgc ttttgacaac gagctggccg gaaatcttca aagcgtccag tttgcggatg
     8161 ctcagggaaa aactctcgtc ggcgcgaacg acccagttgt ccgcctcgtt cggctgactc
     8221 agcgcgccgg aaaccgtaaa cttcgcttcg gaataacgat tgtaagcacc gaaagccgtc
     8281 acccggggca tcagaaccga ccaggctttc atcctgccca tcttcgcgat gtagagattt
     8341 tcctcggcat attttatttt ttcggaattt ttcacggcct cgatatacag gtcccgcagg
     8401 gtgtattcct gcgcgatacc cgccggagca tacaacagta tcgcggccag cagaacgatc
     8461 gggaatgtcc gtaattgctt cactcgggga tattcttttc cggccaaaaa acgtttgcct
     8521 gtcagtgggt gacgcccatg gctctttgca ggcgggcata agcgatattg taatcagcca
     8581 gggcattggc atactcggcc ttggctttgg tcagcagggt ctgcgcgtcg agcacttccg
     8641 tggaacgcgc cacccgctct ttgtaacggg cttcggcaat gcggaaattc tcttcggcct
     8701 gttcgatcac ttttttcgaa acggcaattt ggctttcggc ttcctgcaat gccagaaacg
     8761 cgttcttgat ttccagggcg atctgatcgc tcaattcccg ggactgatcc tgcgcctggt
     8821 tttctccggc ttttccggca tccacgcggt atttcgtttt gccccactcc caaaaattcc
     8881 agttggccac cgccatcacc tgccagcttt cccgatcctt atactgcgtg ccggaaagcg
     8941 atgtcgaatc gccgaaccgc ccgtaatttc caaccagact gaccgctgga aaatattcgc
     9001 tctgcgccgc gcgaaccatt ttgcccgcct gttcaatctt gagcgcggac attttgagtt
     9061 ccggacggtg ctgcctggct atctccaggc attcttcaaa cgattgccgc aggggctgac
     9121 aatcgagcat atcgaccacc tcgacggcgg tccgtacgtc ccgcttcagg atcgtattca
     9181 accgcgcttt ggtcgattca acgccattct tcgccttcac cagtgtctgc cggccgttgg
     9241 cgagctccac ttccgcttgc agcatgtcgt ttttgggaat cagaccgacc cgataaaaat
     9301 tttcggccac atcgcagtgg gcggcaagca tttccacctg ctgtctggcc gtctgtgtca
     9361 gcctttgcgt gcgcaaaacg ttgaaatagg cgatcttgac atcctggatg acatccaggg
     9421 atttggccgt ctgttcgacg agcgccgtat cctcggcaat tttgaaggcc tggtagttgg
     9481 ccaggatccc gccgccagca aaaagcggct gacgggcctc gatgccccag ttgtagttgt
     9541 caatggttcc ggtaaccatg cgacctgcgg ggattccggc aagcggaaac cccggaaagt
     9601 aaaagttggg ctcttcattg aggcgggtgt aactgtaggt cgtgctgaat ttcggcaaaa
     9661 aaccggtcag ggcctccttc ctctgcgcct gcgcgccctt gaccccctct ttggcgatgt
     9721 ccagcacata gctgttcttc agcgcaatgg caatgctctc ctccagggtc aacgcctggg
     9781 cggataccgc agagggggaa aacagcagaa aaacggccac gataactatc cgcaacccct
     9841 ggcacttcgt cgtcattatt tttgctccat tttcaaattg atgcaacatt tagtatattt
     9901 tcgacaaaaa aataagcatt aaaataattc ttcagcaggc gtggatcaaa cgcggacaac
     9961 ctcttcgatg atgccgccgc ccaatacttc atcggctgta taaaaacgac ggattgcccc
    10021 ggcgtaatcg cttcctggac ctgtgtgaaa acaaccctca ccctgacgtc ttccgggatg
    10081 acgcggcagc gcgccgaatt tcagatgacg gttgcaatcc acacagggat tcggcgtctg
    10141 tccgttttga cactcacgga cgaattggcc gatccctcgc tgttccatca gatcggcaaa
    10201 atcaaaaatc cggtggggaa tcccgagtcg tccgcacacg ctttgagcgt cggcgacaga
    10261 ttcattcgta aaacgcccgg cttcgtcgcc taaccggtga acgcccagca gcatcgtcgc
    10321 cccggcaact gcataacccg cctcccgaag cagcagcgcc gcgacagacg aaaccgcccc
    10381 gccgctcatg gccaccggaa tttttttctt ttccaaaacg ctccgcatgc ctcaccttca
    10441 aaagtgattc tattctttcc atgatattct cccgctttgc aacttttttt caacaggcgc
    10501 ttttccattc ttgcaatttc aaggcaacct tgctattcaa gtgcgtcaaa attctgacgg
    10561 gttgccgtga ctttttaccg gaccggaatg ctgcccaaac ggatcgttct attaggaaag
    10621 acaggaaata cgggggcttg gaatggagat tcactttctt ctttttgttt tgctgctggc
    10681 ggcgacggcc attatcagtt tcgcctacgc gatctggcgg ggccgcaatg accaacagat
    10741 cctccgcagc attctgcaaa gctcgccgat tcccaccttt gtcatctcca ccgaccgtaa
    10801 aatcctctac tggaaccgcg cgctggaagg gctctccggc atccgggcgg aggaagtcct
    10861 gggcaccgac cagcaatggc gcgcgtttta ttcttcaccc cggtcctgca tggcggacct
    10921 catcatcagc aataaaatcg atcattccta ctctctctac gccggaaaag tcaccaagtc
    10981 aaaggtcctg aagtatgcct atgaggtgac ggagtttttt ccggatctgg gggaaaaggg
    11041 gaaatggttc cggatcaccg caacgagcat ccgcgattcc agcggcaaaa cgttcggcgc
    11101 catggaaacg ctggaagaca ttaccgatca gaagacggcg gaggaagaac tgctcaagcg
    11161 gaccaagctg gaatccctgg gaaccttcgc cgacggcgtc gccaaggact ttgacagcct
    11221 gctgaccgcc atcctgcgca atgtttttct ggcgaaaatt tccgcgagcg aagaggataa
    11281 aattctggaa aacggcctgg ccgtcgcgga gcgcgcgggt ttgcaggcca aggagctggc
    11341 gcaccggctc atcaccttcg cacagggcgg ctacccggtc aagaaaattg aaaggatcgg
    11401 cgatctgatc cgcgagaccg tccagaaaac cctggccgga tcgaaggtaa cctgcgctcc
    11461 cgtgattgcc gccgacctct ggcccgtgga agtggacgcc ctgcaaatca aacaggtgat
    11521 tgagaatatg gtcaccaacg cgaaggaggc catgcccgaa ggcggcccca tggaagtgat
    11581 cgcggaaaac accacgaccg gcaccaatat ccggacgctc ggcgagggca actatgtccg
    11641 gattgtcatt cgggacaagg gcgtcggcat cccccgggag aacctctcca aaattttcga
    11701 tccgtatttt acgaccaaag aaaagaagga gcgtggcggc atcggcctgg ggctcgccat
    11761 ctgcgattcc atcatcaagt atcacaacgg gctgatcagc atggaatcga cggtcggcgt
    11821 cggcacaacg tttttcattt atctggccgc catgcccaat ggttccccaa aaagcaacgt
    11881 cagctaatta gacctctcgt gttccttctt gacgccgtcg agcagttcct caatatggcg
    11941 cagccgctcc tgaaacgtgg caatgcccag cgccgactcc cggggatgaa gattttcaat
    12001 ctcggcaagc cggttgcgca gatgcaaaat gatttcctcc gccagacaaa acagcagagc
    12061 gcgctcagat ctggtcatga cccgaatacc ctcctattcc tgctgcatgg acttccggag
    12121 cagaaatgcc ttcttaatgg actccgccgc actttcaatg ctgactttat ccgtattgac
    12181 catcatgtca taatgcttcg gatcgttaat atcttccttg aagtaatttt taataaagcc
    12241 cacctgatat tcatcatact tgcgcacata ttccagtgcc tccgctttcg acatgtggcg
    12301 atcccgcatc actttctcca cacgcgcttc ctccggcgcg atgatccgca cgcgaaaggc
    12361 cttgtctttc aaaataaagc ttccgccgcg gccgatgata ataacattgc cgtgttccgc
    12421 gttcgtgtgc atcaccatcg acaaatgccg gaggtattca ttgggccaga tatgccgcga
    12481 ttcaaagaag gaactgatca ggctgtcgaa aaacgagatg cttttttcgt ccagcgattt
    12541 gatcaccttt tcgctcatgt gcgcgctttc ggcgacttta tgaatcaatt cgcttcccac
    12601 gatgttcatt ttcagctccg tggccagaat ccgggaaatg aagccccctc ccgctcccgt
    12661 ttcgctggac agcgtaatga catgccccgg tttttccttt ttgtcccgct gccagcgcat
    12721 gacctggtct tccaccagtt tgtcaacatc cttatcccgc aatttgatat cccgggtata
    12781 atcggggcaa ttgacatcaa acagggcatt ggttttcacc cgataccgct tggtgcagtt
    12841 ttcgcgccac gcgcagatca cgcaaattct tttgtccgat cctgttgaca tgcgccactc
    12901 cccctttctc gtttgatgtt atgacgcctc ttcctgcagc gcctcaaatc cggcaacaat
    12961 atcaagaagc tcttcggtaa tcgtcatctg gcgggtgcgg tggaactgca cgtgcagatc
    13021 cagcaactgc tcttcaatat tcttctcggc gttctgcatg gacgccaggc ggctggcgtt
    13081 ctcgctggcc agcgactcgg cgaaggcccg gtacagcgac acgaacaaat actcgcggat
    13141 cagcgacgaa aagatcttgt cgccgtccat ccggaagatc ggcagagatt tggattccca
    13201 ttttttcctg gcgatctgtt tgagccaggc ctcatccacc ggcaaaagct gcagcatgtg
    13261 ggggtggtac gtcgctccgg tcaggtattc attataaaaa agaaagaaat gatcgacgtt
    13321 gccgcgaaaa tgccactcat cgataatcat aataatttcc tgcaccagcg gcgtaatgcc
    13381 cgccgtggag ccgggagtcg aaagcagctc atccaccgtc tgtccggcgt cttccacata
    13441 gtccgccacg cgcgcgccga ccgaaagaac tttgcggttt tcttttttca cgcccgtgct
    13501 tttgatatga tccagcgcga atgtggaaat ctgctcattg agctgtccgc acagtccctg
    13561 gtcggatccg aacacgatca cgccgagacg tttgatggga acgttcttcc gcttcatcgt
    13621 cgcgcccatg cgctccttga gcacgatctg caggcccatt tccaccgtgc ggttgtaatc
    13681 acgcagcgaa tcgacggccc gctggtactg gcggatgctc acggcagcca gggctttcat
    13741 cgtcttgacg acggataaaa ggtccccggc gcttttcatt ctccttttca gcgactcagt
    13801 tgtttgcatg ggcttcctca atctcttcaa acggagcgat cgtcggtttg attttgttca
    13861 tgatgctgtc gcggtccgtc atatccatct ttttgccttc ttcaatccgc gtgcacaatt
    13921 ccggaagctg ttcgttgacc gtttccagca ggcgcgcctc cacctcgcgg atcttttccg
    13981 tcggaacgag atccagggcg cctccggtta cggaaagaag agaggcaatc tgttcggagg
    14041 cccgcaacgg cttgaactgg ccctgtttca ggatttcacg gacgcgccag ccgcgctcca
    14101 gcgtccgccg cgtggcgtcg tcgagtcttg tgccgaaccg ggagaaggac tccagttctt
    14161 caaactgcga gtaggacaga cgcagatcgc ccgccacgga tcggtatgcg ggaagctgtg
    14221 tcttgccgcc gacgcgggaa acggacttgc cgacgtccac cgccggcaga atccctttgc
    14281 tgaagagcac cggcgaaaga taaatctgcc cgtccgtaat ggaaatcaga ttggtgggaa
    14341 tataagacga catgtcctgg gcttccgttt ccgtgatggg aagcgacgtc agcgacccgc
    14401 cgcccagttc cggccgcata tgcgtggagc gctccaagag ccgtgagtga atataaaaga
    14461 tatcgccggg aaacgcttct cttccggggg gccgccgcaa aagcagcgac acttcgcggt
    14521 acgcccgcgc atgggaggtc agatcatcat agacaatcaa aacatcccgg ccctgctcca
    14581 caaaatattc ccccatgctg gtcgccgcat agggagcaat aaactgcagg cccggtgtgt
    14641 cttcgccggt ggccaccacc acgatgcagg atttcatcac cccgtgatcg cgcagttcgg
    14701 cgatcacctt ggcgacatcg gcgcttttct tgccggtcgc gcaataaatg gagatgattc
    14761 cggtttcttt ctgattaatg atggtatcga cggcaatcgc tgttttgccg gtcattctgt
    14821 cgcccagaat caactcgcgc tggccccgcc cgacgggaat gagcgcgtca atcaccttaa
    14881 tgccggtctg cagcggcacc gtcaccgggg cgcggtccat caccgcgggc gcgggccgct
    14941 ccaccggcag gcgcatgacg gtgttcactt cccccaggcc gtccagcgga cgtcccagcg
    15001 gatcgaccac ccgccccagc aggccgtcgc ccaccggaac atccaggacg cgcttggtgc
    15061 gctgcacttc cgaacccacc tggatatttt cactgggatc cagaagaatc acgccgattt
    15121 ccttgggatc gatgttgaac accagcccca tgtaattgcc ggaaaatcgc accagttcct
    15181 ccgcgcggat atgcggcaga ccgctgaccc gggcgatgtc ccggccgaca aactgcacgg
    15241 tgccgatttc gtattgtctc agttcaggat tctgctcttc caaaaccttt tccatggtat
    15301 cgaacgtgtc gtccagaaag gttttcagct cctgactttc cacttggctc acctcttgat
    15361 tcattctacg cctcctgaca tccttccaga cttcagcctt cggccttccg gtcgataaac
    15421 ctttctatgc cgcatgcgct tctttctgga gcgtttccgt caaactctcc accagggttt
    15481 ccagatattc gttcaggctc caggcgattt tatggccgtt gacccgcatt tcaatgcccg
    15541 aaacaatatc cggttcttgc agataacgga tggtaaatcc gttggtgatc tgcttcttca
    15601 ggacttcttc aatctgcgtc tgcctcgggg caggaatgcc gaaagcgctc tggatcgtca
    15661 cccggtttcc tccgccgctg atcgccttgc gcagctgagt gcttttttcc tcgtccagcg
    15721 actgcacccg gcggaggaat tcctcgacga ttctctcctc cagatcggcg tccgccagat
    15781 cggaaagggc tttgcgcgcc gtggcgtaca actgcttggc cgctcgctga cgcaaatcat
    15841 agaaaaaggc gtcctgctcc cggatcagca tgtcctgcca gcgcgctttg accgcatcca
    15901 cttccacgcg cactttttcc atcagctctt tgcgtttggc ttccgccgcc agcgtcgcct
    15961 cgttgagcat ggtttctttt ttctcattga ggctctgatt gcgcttttcg tagagcaggg
    16021 cttcttcttc cgctttcgtc ttcagctcat ccgcttcggc gaagcgcgcg gcgattttcg
    16081 cctcccggtc atccatggcc ttgataatcc gcccgtagag aaagcgtttc agcagataca
    16141 tcagcaggag gaaattgaag atttgcgcta aaaatacaaa ccagtcgata tgcatggttt
    16201 atccccctac ttgataatga aaccccagaa cggattggcg aaaatcagaa tcatggaaat
    16261 cacgaaacag taaatggcaa tggattcaat catggccaaa ccgacaaata atgttcgcgt
    16321 taaggaattc ctctcatccg gctgctgggc aatcgcggcc agcgcctggg cgattgcctg
    16381 tcccatgccc agtccgggac cgatcgaacc gatccccatt gccaaacccg caccgataat
    16441 cgaggccatc gcgacgagac ttacgttgtc catacatact cctcctttat ttcaatttta
    16501 tcaggctgca gagccctctt ttttggcttc cgtgtgatgt gcttcctcct cggcggaaag
    16561 cgccgaggca atatagacca gggaaagaat ggcgaaaatg taggcctgaa tcacgccgat
    16621 caggagcccc agaatctgca tcacgaccgg gaacagaaaa ggcgtgaccg ccagcaatat
    16681 gccaatgact ttttcatgac tcataatatt gccgaaaaga cgaacggtga gcgccagtgt
    16741 tctggacaat tcgcccatga catgaaacgg aaagaaaata aaagtcggct gaaaatattc
    16801 cttgacgtaa tgcagaatgc cgttccggga tatcccgtag tacggaacgg caatgaagac
    16861 acaggtcgcc agcgccgcag tggttgttat ggaagacgtc ggcgcgacat atcccggcac
    16921 caccgccagc acattggaag tgcaaatgaa cagaaagagc gtgccgatca gtgggacata
    16981 actgtccgcc cccttcttgg tcatttcact gatttccccg cgcacgacgg aaatcacgac
    17041 ctccaggaag tgctgccaac gcgacacatt catttcggaa gaaagattgc gggtgaccag
    17101 aatggagccc ccgaccagaa tggccatcac aagccaagtg taaaggatgg tcacgttgat
    17161 ggtgatgaag ctccagctcc agagcacgac ctgatcgggg ctgatttgac ttaactggac
    17221 gatttccata gaatctcctt caacggttga tcaaaaacct ttgcatcact cctgtattgc
    17281 ttccgccgtc ttttgcggac acagacgaac cgtcagaatt ttgcgcatca ccacgaaacc
    17341 gatcagggct gcggccagcc tttcccagcg gccgtcttga agcaaaaagt aaaacacggc
    17401 caaaaggacg gacaggcgcg ccagcaggct gaccagcaac aggcgggcgc ggctttccgt
    17461 ttgaggaagc ctcttcacgg tctgccacag gccgacgaaa tagagagcgc ccagggccat
    17521 tcccgcggca aacattaaaa caagcatccc catggcgttt agcgtcatgt cattcctctt
    17581 caatgatccg gctgcgaatt tttcgaaccc aataggctgc gttgatgcag ccgacgatca
    17641 caccggccac cagccccatc agcgcccacg aatactggct gggccacgtc cgatcgatcc
    17701 aaagccccaa ggctacgcca atcagcgtgg gaatcgccac cgcccatccg acaatgccga
    17761 acatccccag accgaaccag accgtttcat ttttatgttt gagacccttg atgcgcagcg
    17821 cctcccgctg ggcgaccttt tccgcaaagt gatccgccat ctgccggcgc cggttggtgg
    17881 ttcgtcgtat ttcaaccatg tttttactcc cttgttcggt gcgttcgggg aacgcgccct
    17941 gccatgattt catcggcgcg ttcggggaac gcgccctact tcagctccac gaagcgccgg
    18001 atcagcgacg cctccagttt tgccgcggcg gagcggacca ttttttcccg ttcgtcgagc
    18061 gcgtcatact gctccacaac cacctgcttg agttttccca gctccgccgc cttcacggca
    18121 ttgcgggtgg acaccaggac atccgctccc tttttcacca gcgtgccgac gtccatggcc
    18181 agaagctcgt gaccgccttc ggtcggctca tacgaaaaga tgcccggcgc cagcgtagcg
    18241 acaaagtcga tatggttcgg catcaggcag aaagaaccgt tttccgcctc cgcgacgatt
    18301 ttcttgactt cccgctccat catgatttcc gccggcaata atattttaag caacattttc
    18361 tatcttcgcc tcttcaatcg gtccgatcat gtaaagcgcg cgctccggat attcggcaaa
    18421 ctcatcgttc aggatccgct cgcacccgtt gagcgtctct tcccgggaaa ccattttccc
    18481 ggctgtgccg gtgaattgct cggtaacaaa gaaaggctgc gtgaaaaacc gctccaatcg
    18541 gcgcgcgcgg aacaccgtgc gctggtcttc gcgggacagt tcggaaatgc ccagcatggc
    18601 gataatgtcc ttgaggtctt catataccgc gagggtcttg cgtatttcct gggcgatttt
    18661 gtagtgccgc tcaccggcaa tgttgggcat cagcattttg gagccggact gcagcagatc
    18721 gatggcggga tacaacccct cgctggcccg cttgcgcgaa agggcgatgg tcgcggacag
    18781 gtggccgaag gtgtgtacgg ccgatggatc cgtaaaatcg tccgccggaa catacacggc
    18841 ctgaatggag gtgatcgctc cggttttggt gttgcagatg cgttcttcaa gctccgccag
    18901 ttccgtggcc agcgtcggct gataccccag acgcgacggc agctgcccca ggagacccga
    18961 aacttccatg ccggcctgga tgaagcggaa gatgttgtcg atcatcagaa gcacgtcctg
    19021 cttggcatcg tcgcggaaat actcggccat cgtcaacgcc gcgtgcccta cgcgaaaccg
    19081 cgcgccgggc ggttcgttca tctggccgaa gaccatgacc gtgttcccca gaacgccgct
    19141 ttcctccatt tcccggtaga gctcctctcc ttcgcggcag cgctcgccga taccgcaaaa
    19201 gatgctcatg ccttcgtgac cgctgacggt attgtgaatc atctccgtaa tcaaaacggt
    19261 cttgccgacg ccggcgccgc cgaacaaccc ggccttgccg ccccgttcca gaggcgccag
    19321 gacgtcgatg gccttgatgc cggtttcaaa gatttccgag acggtggact gatcgcgcag
    19381 cggaataggc tcgcggtgaa tcgatcgggc ctcgcacccc tcaacattac ccttcttgtc
    19441 gatggtgttg ccgaagacat tgaacaccct tcccagcaac tgactcccca cgggaacctc
    19501 aaagggtttg ccggtatcgg tgaccacggc cccgcgggcc agaccctgcg tcggcgtcag
    19561 ggcgacgccc cggatggtat tgttgtccag ttgtgtaaac acctcgatga cgacctcacc
    19621 cttctctccc gccgtcagca cattgcggat cggcggggca acgggaaagt ggatgtcaac
    19681 gacgctgctg cgaacggata aaacccttcc ttgatttgtt tgctcagcca cggtttgttc
    19741 acctcgttta ttttttcttg ggagcggctc ccattaatgc catgtattta tccgtcag
//
LOCUS       DAOSRM010000038        19789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_2914_length_19789_cov_101.750076, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000038 DAOSRM010000000
VERSION     DAOSRM010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 19789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19789
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2914_length_19789_cov_101.750076"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1158
                     /locus_tag="PLA58_05350"
     CDS             220..1158
                     /locus_tag="PLA58_05350"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOR62254.1"
                     /translation="MGENIGEYSNNIVDLRRHMWMAGVEVELRRLIWQIAIVGKYISA
                     KIHESNRKLAGFKNIYGEEQLALDRVSDDILKNKLQHSGFVSHYASEELDDIVAIGNG
                     KEKYIVTADPLDGSSLVDTNLAIGTIIGIHQNTIFDCGRNSMVAALYITYGPLITMIY
                     SAGRGAHEFVLDREGEYVLSEENIRLKEKGSIFSPGGLRRDWTDAHLKFIGKLESDGY
                     KLRYSGGFVPDINQVIIKRGGLFTYPALKKSPQGKLRLLFELQPMAFIVEQAGGRATD
                     GKDDILSLKVASVDQRAPIYIGSAAEVKMAGEYLSQ"
     gene            1182..2576
                     /locus_tag="PLA58_05355"
     CDS             1182..2576
                     /locus_tag="PLA58_05355"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOR62255.1"
                     /translation="MIYENKDQLKKGCEATLSHEGGDVRILDEERFRSRLIDDLIQTV
                     AFSPDQGTREAAGFLIRRGAAALGIVSSSIQALYDAMGRGEVGGFTVPAINIRGISYD
                     VAQAVFRAAMAGNVGPVLFEIARSEIDYTRQRPLEYTCAVTAAAVKTGWRGPIFLQGD
                     HFQVNAKKFAADSEKETQAVKNLIWEAIEGGFYNIDIDTSTLVDLSKPTIKDQQRTNF
                     SLAAELTTMIRDLEPEGITISVGGEIGEVGGKNSTVEELQVYMEGYLEELKKRGDAFK
                     GISKISVQTGTTHGGVPLADGTVAKVKIDFDVLEKLSEVARTKYGLSGAVQHGASTLP
                     DEAFDRFPATGTAEIHLATGFQNMIYDSKVFPADLRTRIYDYLKTNMKGEWKEKDTEE
                     QFIYKTRKKGFGPFKLELWHLPADIRAAICAELERQFAFLFDKLKVNGTRELLDKYIK
                     SIDVPLKMPPAIKG"
     gene            2698..3735
                     /locus_tag="PLA58_05360"
     CDS             2698..3735
                     /locus_tag="PLA58_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOR62256.1"
                     /translation="MKAAVLESITNIREHPRPLSFVDLPDPEPFEGQILVRVSTCGVC
                     HTELDEIEGRTPPSSFPFILGHQIVGIVEKCGPRALKFSAGQRVGIGWIGSACGTCNY
                     CRGGMENLCPDFRATGRDIPGGYAQYTTVLEDFAFPVPDAFSDAQAAPLLCAGAIGWR
                     SLRLAGLADGEPLGLTGFGASAHLVLQLARYLYPRSKICVFARSERERNFALSLGAGW
                     AGEPAEKSPEKLAAVIDTTPAWKPVVEALANLAPGGRLVINAIRKEASDRDQLLRLEY
                     PLHLWQEKEIKSVANVARRDIAEFLDLAAKIPILPDVEEYAFFDANRALAELKAGQIR
                     GAKVLKIGNGL"
     gene            complement(3811..4659)
                     /locus_tag="PLA58_05365"
     CDS             complement(3811..4659)
                     /locus_tag="PLA58_05365"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR62257.1"
                     /translation="MTPVSDIIRRRFSCRSYENKPLADNLLRTFRQAVEASARGLFGN
                     TPRFVLLEMDELPRDAWKKLGTYGVIQNARLYIAGMIRPAPMATVDYGYCKEKLILKA
                     TELGLSTCWLGGTFAISAFGRAAGMRRDELMPTVSPVGYAAGRRSLTERLLRGFAGSD
                     HRLPWPELFFRGNAATPLPVEAAGPYAEALENVRLGPSASNKQPWRIICDPKQGAFSF
                     YLSRSAGYRHLRDVSLQEIDMGIALCHFDLTVRELGLAGIWRQEEPPAGIKSWEYVAG
                     WRAEGK"
     gene            4763..6334
                     /locus_tag="PLA58_05370"
     CDS             4763..6334
                     /locus_tag="PLA58_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOR62258.1"
                     /translation="MKLATVEQMREMDRQAIEKLGIAEEMLMENAAHAAVSLLQNAFG
                     IRDRNFVIFCGIGNNGGDGLAVARLIKSAGGRAKVFLTASPKKYTGAAKLNYDILCNL
                     DADVQLLSRAQEARVDLLAADVVVDAIFGTGLDRDVRGLAADVIALVNACGRPVVSLD
                     IPSGISGNSGQVMGAAVRATHTVTFGLPKLGNLLYPGYEHCGALSVTHISFPPSLYQG
                     DALTVETNGTVAPLPPRPAQAYKSSMGDVLFIAGGGNYFGAPYFSAMSFLKSGGGYAR
                     LASPASIIPFVAQSGREIVYLPQRETALFGLSLKAKPDLLALAEKADMVVMGPGLSLQ
                     EETTKLIRELAAVISRPLLIDGDGLTAIAERPEILLKRKAETILTPHAGEMARLTRKT
                     VACVNADKIGVLREAAKKLNATVVFKGPHSLVGLTDGRVYVNLSGNPGMATAGSGDVL
                     TGCIAAMHGLGLQPALATRKGVFLHGCAGDLAAAEKGQDGMTATDILEHLPQALKNDR
                     EGAADEKHYEPPVLL"
     gene            6448..6870
                     /locus_tag="PLA58_05375"
     CDS             6448..6870
                     /locus_tag="PLA58_05375"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOR62259.1"
                     /translation="MAGIPHLQWSQSLSVRVDGIDAQHKKLFEITNHLIDVFEAGSGD
                     FLTVISELVEYTTVHFHDEQMVMMNAKFPGLAAHTAEHDKFIAKVEEFLQAYGEGNED
                     VGSKMLVFLRDWLFAHTSGLDMEYAQFLAKNSAKTKAF"
     gene            6906..9185
                     /locus_tag="PLA58_05380"
     CDS             6906..9185
                     /locus_tag="PLA58_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOR62260.1"
                     /translation="MADMSKVAGLQDVLRQQKGLQSITSRINAATGIRQMIAEICPLL
                     IDLFRVEAAHVFVREKNRQDVYTFTGQTGDVKTVLGPQTLPGYIANEGKMIHISDFNK
                     SDQIEAYRDFPVSGGGDSRFGLPVRQILAMPIFYGGETLGAVEVLNKKEDGPFGEKEP
                     ALLQNISELLGVALYNFLLVDPKAKRVKGAYLVSQGLILQEEMDRAKEESRVTKEPLQ
                     DVLVKKFKISKEDVGRACEFFWQCKFVSFDKKTPIPGELLKNLKRDYLIREVWVPLER
                     TDKGVRVIMDDPQDILRRDAVEKSLKAKSVQYDVALKEDILQFIDLFFQESAGESTFS
                     ELLDKLEPGESERKEETEDVDESDSAIIQLVNKIISDAFNRRASDIHIEPNVIDKNVL
                     VRFRVDGDCALYQTVPYSYRSALISRIKIMANLDITVKRIPQDGKIKIKRSGAEEIEL
                     RVVTIPTQGGVEDVVMRILAKGDALPLSAMRMSNRNYQDILKICEQPYGMILVVGPTG
                     SGKTTTLHAILNHINKPDRKIWTAEDPVEITQRGLRQVQVQPKIGFDFAAAMRAFLRA
                     DPDVIMVGEMRDFETAKIGVEASLTGHLVLSTLHTNSAPETISRLLDMGIDPLNFADS
                     LLAVLAQRLVRTLCGNCKEAYHPTREEYDQIAQSFGAEYFAKQNLPYSDDLTLYRPKG
                     CDACDQTGYKGRIGIHELLLNSPEIRKLAIDRQESIETLRKVAMNQGMLTLYQDGILK
                     AFQGLTDIKQVHRVCVTSK"
     gene            complement(9232..10206)
                     /locus_tag="PLA58_05385"
     CDS             complement(9232..10206)
                     /locus_tag="PLA58_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOR62261.1"
                     /translation="MDLSDIFSLYKNEMHKVETHLDRYITSDVRLIPDVAHHLIDSGG
                     KRFRPLLHLICARLCGYDGPRRFPLAASIEFIHTASLLHDDVIDEGLLRRGKTSANRV
                     WGNAASVLVGDFLYSKAFSLLADIGDIRVVQLMSKMTNVMSEGEVFQLMKCGDTSLTE
                     EEYLSIVEKKTAVLISSACESGAALGGAPESQARALGQYGYNIGMAFQITDDTLDYMA
                     REEDFGKSIGKDLEEGKMTLPLIYTLSHCPPDRREKIKTAIEKKDLPSEAIRNIFEVI
                     QQSGGIHYSLQRAEQFITQARSGLDVFPASPEKEHLTAVAGYILSRKI"
     gene            complement(10240..11616)
                     /gene="rimO"
                     /locus_tag="PLA58_05390"
     CDS             complement(10240..11616)
                     /gene="rimO"
                     /locus_tag="PLA58_05390"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOR62262.1"
                     /translation="MKKLLSGKLHILSLGCSKNLIDSEVMGGLARASGMTMAERPQDA
                     DVVLINTCGFIGPAKEESLEAILTLAQIKKQRGRDLKLVVAGCLAQRYGKALSSEIPE
                     ADLWIGTGEVGRIAEHLRRLDAGGTRRKAIVSKPDFLMTSRHERLLPSGAATAYLKIS
                     DGCSNRCAYCAIPSIRGRARSRRPDDILREAEILVGRGIREIILIGQDTTAYGSDLKN
                     RPTLSGLLSDLSAIPGLRWLRLLYAHPAHITPDVLEAMAGRENICRYLDLPVQHIDDA
                     ILHAMNRRVSSEMIREILRQARSLMPDLALRTSLIVGFPGETQKRFERLLDFVAEARF
                     DHLGVFTYSREEGTPAADLPSRISEKEKDRRHDAIMSEQADVSYAVNQTLIGSVQEVL
                     MEGISDRPDFARVGRCRRQAPDVDGVTYLKKGPAAIGDFVRCRITGADHYDLFGEILF
                     DNYRKKPL"
     gene            complement(11613..13859)
                     /locus_tag="PLA58_05395"
     CDS             complement(11613..13859)
                     /locus_tag="PLA58_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOR62263.1"
                     /translation="MEETSQHPLPARFREIKGVLCLALSLFLFLCLFSFSPEDPAPLL
                     KSVGEPSSTHNWTGIVGAYTARWMIFLLGLASFLLPASSLALAFQFFRRPEFSLKTPR
                     VFGFLSLTLSLAALSDALIRGGVTVYGATFPSGGILGAGLVRFLQGYFNPAGTFILLI
                     AWMMVSLFFIIDFSLVSTTEKFLQSVREGLSAFKGRAVSFFTGWFARAKAEKSPPPVI
                     EDAAAKAPPKKAKTKKVEQTHFDFAKLPSDGAFQLPPFSLLSEPPQKDARVKRDHLIT
                     NSRILEKKLADFGVEGRVVEVLPGPVITMYELEPAAGVKINRITSLTDDLALALMAPS
                     IRILAPIPGKSVIGIEIPNLKREPVFLKDVLDNDAFTESSLRLPIALGVDFVGAPVIA
                     DLARMPHLLIAGTTGSGKSVALNAMICSILFKATPDNIKFLMVDPKRLELSSYEGIPH
                     LMHPVVVDPKKASQVLRWTVEEMERRYQIISELGVKSIDAYNEIIITRGKARTAAKEP
                     KPEPRPDRDPAEDSAESDAPFQHVTLPYIVIVIDELADLMMVAPRDVEESLTRLAQMA
                     RAAGIHLILATQRPSVDVITGLIKANFPTRISFKVSSKIDSRTIIDQPGAEQLLGAGD
                     MLFIPPGTSKLTRIHGAYLSDAEISRIVEFIKMQAQPIYDASIEKFELESGQTRQDED
                     GYDEKYDEAVALVGELGQASISLVQRYMKIGYNRAARMIEQMEREGIVGPSDGAKPRK
                     VLIRKLPQ"
     gene            complement(13859..14620)
                     /locus_tag="PLA58_05400"
     CDS             complement(13859..14620)
                     /locus_tag="PLA58_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR62264.1"
                     /translation="MIRSAVLVSIGVAITAFFSFWAVVFSFFANAENNVHKVANIWSR
                     ILLAICGTKVRVIGRENLLRGKPQVLMANHQSDFDILITLAHVPGQFRWIAKKELFAI
                     PVFGEAMKSAGYIEIDRQNHEKALRSLELAALRIREGKSVMTFPEGTRSRDGEIKAFK
                     QGTFYLAIQAGAPIVPISIIGSGEIMPKRSLKIKPGKVKLIIDKPIPVAGYSLENRHE
                     LIDRVRQVIIRNYDAYRDAGKAGIKDVQTDTKTAL"
     gene            14993..15862
                     /locus_tag="PLA58_05405"
     CDS             14993..15862
                     /locus_tag="PLA58_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR62265.1"
                     /translation="MELFLQQVFNGIMLGSTYAIVALGLTMVFGILHVPNFAHGQMYM
                     LGAYVCFFLITIFGFAYWPAMAMSAVVLALGGVILEVLVFKPVRNAGGIAPFISALGA
                     LIIIENAVVVLWGPEGHRIPNPYPGIIEVFGITMSTQRLLVIGVAFALIVLLQIFIKK
                     TTLGSTIEAVAQNKEGATLVGINVNRVSALTFAISTGLAAVAACLVAPIFMISPSMGM
                     MVGMKAFVIVILGGLGSIPGAIVGGLILGLIEAIGGGYFSAAYKDVFGFGALVLILAI
                     RPTGLFGKEIKGR"
     gene            15859..16836
                     /locus_tag="PLA58_05410"
     CDS             15859..16836
                     /locus_tag="PLA58_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR62266.1"
                     /translation="MMKWLKTNWFYLVVLVIALALPLVITNRYYSQVITMSLLFAIGA
                     LSLNLILGFTGQMSLAHGAFFAIGAYGVAILTYTLGWNFWLALPSSALLAAFIGFLVG
                     MPALRTRGSYFAITTMCLGEIIYLIAGNWMELTGGHNGIVGIPVPSPIAVPGVLEITF
                     QSQASQYYLVLFFLLLTVFVMHRLTKSLRGMTFLATALNEDLSEAVGINTFRTKLLSF
                     IVANFFAALAGGIYASLIGSISPSVASLTMTFNFLMFVLLGGITTLAGPIIGAFIIPI
                     LLEYLQFLQDYQMILFGFLLIVVIIYFPTGFMGLYAKVRDKIKKSRVGA"
     gene            16838..17602
                     /locus_tag="PLA58_05415"
     CDS             16838..17602
                     /locus_tag="PLA58_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR62267.1"
                     /translation="MLLKGENLVKRFGGTIAVNEVSFEVHEGEILSIIGPNGAGKSVL
                     FSLISGAGKPTSGSINFDGHEIAGLSASQISRKGIARTFQLTALFEKLPVVANLALGY
                     YKCTTSGFWGGILHTPRWQRDRKKTEEKVLETLEFVGLGAKAFDMASTISQGEQRLLS
                     IGIALISDPKLILFDEPTGGLIQEDTAMITELIRKINKRGTTVCLIEHKMRMVMDLAD
                     RIVVLNYGKKIAEGKPADVAADPQVIEAYLGRKKNA"
     gene            17595..18299
                     /locus_tag="PLA58_05420"
     CDS             17595..18299
                     /locus_tag="PLA58_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR62268.1"
                     /translation="MLNLTNVTTMIGEHQVIHDVSLHIAQGDFVALLGGNGAGKTSLF
                     RTIVGVLPPAKGKIEFKGEEISHIAAHKIVGKGIALCPEGRQLFPQLSVKKNLMLGAY
                     HRKDKKGIEESLEQVYALFPRLKERYKQMAGTFSGGEQQMLAIGRAIMSKPELLLLDE
                     PSMGLAPLVVEGIADAITQLNKMGITVFLSEQNAQIALMITRHGYVMENGHLVLEGLS
                     KDLITNEKVRKAYLGT"
     gene            18487..19035
                     /locus_tag="PLA58_05425"
     CDS             18487..19035
                     /locus_tag="PLA58_05425"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOR62269.1"
                     /translation="MKNRSKWFSALMISLLLLYGQGSFAQGPRAKKAASAKVVRVSGA
                     QAWECAACHRAQNVLPKQHVDIQEMPYVGCVVCHLSEEGFAGALKGKMPGSHLHAFRN
                     VPCAGCHGQEKKPAPVAKSQCLVCHGSAAKVALKTQNTKPNNPHDSPHYGTQLDCNFC
                     HRQHAKSEDYCAQCHKFNFVVP"
     gene            19072..>19789
                     /gene="nrfD"
                     /locus_tag="PLA58_05430"
     CDS             19072..>19789
                     /gene="nrfD"
                     /locus_tag="PLA58_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOR62270.1"
                     /translation="MGCPENSSWTGCLKDAGRRVFSGGPVYLFFLGLFAALLVAGVAA
                     GIHSVYIVGSRHAYGTTREIPLAMLIATYTFFVITSTGLCLVSSIGHIFGVERFMPIA
                     KRAVLLSVITIMSGFLVIGLELENPLRLAVYLFCSPNFTSNIWWMGVLYAFEVVLLIL
                     EFIFLLLDKHKASVAAGLLGILFGIAAISNLGGVFAMLNGREFWYGPYLPIFFIASAL
                     LMGCAAVIFFTVLAYRVHREP"
BASE COUNT         4234 a         5410 c         5869 g         4276 t
ORIGIN      
        1 acgcgcaaat cagttgattc gatcaaaatc atctaatgga aaatctggga taaaaaaaga
       61 tctttccctg tcaaccaata aacgggagac catgaaatat ttgcttgtaa tgaccaggga
      121 tatttggtta ataaattgac gctgcatgcc tgtggatccg aaacgtgccg aaaaaaatgg
      181 ttctgcaaac cagaaaacga atgatatcaa ggagaagcta tgggcgaaaa catcggtgag
      241 tattcaaaca acattgttga tctgaggcgc cacatgtgga tggccggcgt cgaagtggag
      301 ctgagaagac tgatctggca gattgccatt gtcggcaagt acatctccgc caaaattcat
      361 gaatccaacc gcaagctggc gggttttaag aatatttacg gggaagagca gcttgcactg
      421 gaccgcgtga gcgatgatat tctgaaaaac aaactgcaac actcgggctt tgtgagccac
      481 tacgcgtccg aagagctcga cgatatcgta gccatcggca acggcaagga aaaatacatc
      541 gtgaccgccg atccgctgga tggatcgtct ctcgtggaca ccaatctggc catcggcacg
      601 atcatcggca ttcaccagaa caccattttt gactgcgggc ggaattccat ggtggccgcc
      661 ctgtacatta cctacggccc gctgatcacg atgatttatt cggccggcag gggcgcgcat
      721 gagtttgttc tggaccggga aggggaatat gtgctctcgg aggagaatat ccgactgaaa
      781 gaaaaaggat cgatcttttc gcccggcggc ctgcgccggg actggacgga cgcgcatctg
      841 aagttcatcg gaaagctgga gagcgacggc tacaagctgc gctacagcgg cggctttgtg
      901 ccggacatca accaggtgat catcaagcga ggcggtctgt tcacgtatcc ggccctgaag
      961 aaatcccctc agggaaagct gcggctgctg ttcgaactgc agccgatggc cttcatcgtg
     1021 gagcaggccg gcggcagggc gaccgacgga aaggacgata ttctttccct gaaggtcgcc
     1081 tcggtggatc aacgcgcgcc gatttatatc ggctccgcgg cggaagtgaa gatggcgggg
     1141 gaatatctgt cgcaataata gcagaaggat aaggagcagc catgatttac gaaaacaaag
     1201 atcagttgaa aaaaggatgc gaagccactc tctcccatga aggcggggac gtccgcattt
     1261 tggatgaaga aaggtttcgg agcagattga ttgacgactt gatccagacc gtggcgttca
     1321 gtcccgatca gggaacgagg gaagccgcgg gctttctgat tcgccggggc gcggccgcgc
     1381 tggggatcgt gtcctcgtcg atacaagccc tctacgatgc gatggggcgc ggggaagtgg
     1441 gcggctttac ggttccggcc atcaacatcc gcggcatcag ctacgatgtg gcgcaggcgg
     1501 ttttccgcgc cgcgatggcg ggaaacgtcg ggccggtcct ttttgaaatt gcccggtcgg
     1561 aaatcgacta cacccggcag agacctttgg aatacacctg cgccgttacg gcggccgccg
     1621 tgaaaaccgg ctggcgcggg ccgatcttcc ttcagggcga ccacttccag gtgaatgcta
     1681 aaaaatttgc cgcggattcg gaaaaggaga cgcaggcggt caagaatctg atctgggaag
     1741 ccatcgaagg cgggttttac aacattgaca tcgacacctc cacgctggtg gacctgtcga
     1801 agcccacgat caaggatcag cagcggacca acttctcgct ggcggcggaa ctgaccacca
     1861 tgatccgcga tctggagccg gaaggcatca cgatttccgt gggcggggag atcggcgaag
     1921 tgggcggcaa aaacagcacg gtggaagagc tgcaggttta catggaaggg tatcttgagg
     1981 agctcaagaa gcgcggcgac gccttcaagg gcatcagcaa gatcagcgtg cagaccggca
     2041 cgacgcacgg cggcgttccg ctggccgacg gcacggtcgc gaaagtcaaa atcgacttcg
     2101 atgtcctgga aaaactgtcg gaagtcgccc gcacgaagta cggcctttcc ggagcggtcc
     2161 agcacggggc ttccacgctg cccgacgagg cgttcgaccg ctttcccgcc accggcacgg
     2221 cggaaatcca cctggccacg ggcttccaga atatgattta cgacagcaag gtttttcctg
     2281 ccgaccttcg gacgaggatc tacgattatc tgaagaccaa tatgaaaggc gagtggaagg
     2341 aaaaggacac ggaagagcag ttcatctaca aaacccgcaa gaagggtttc gggccgttca
     2401 aactggaatt gtggcatctg ccggcggata ttcgcgcggc catctgcgcc gagctggagc
     2461 gccagtttgc gtttttgttc gacaagctga aagtcaacgg aacccgggaa cttctcgaca
     2521 aatacatcaa atcgattgac gtgccgctga agatgccgcc ggccatcaaa ggctaaggcc
     2581 tttgagcggg gtgaccatgg atcgcaggga acggtcgcga ccgttccctg ctgttttgtg
     2641 caaaagaaag cggacatctt gggcatggaa aattcatcgg cttgacggag ttgcaaaatg
     2701 aaagcggccg tattggagag catcacgaat attcgggagc atccgcggcc gctttctttt
     2761 gtggatcttc ccgatcccga accctttgag ggtcaaattc tggtcagggt ttccacctgc
     2821 ggcgtctgcc ataccgaact cgatgaaatc gaaggccgga cacccccgtc ttcctttccc
     2881 tttattctgg gccaccagat tgtcggcatt gttgaaaaat gcggcccccg ggcgctgaaa
     2941 ttctccgccg gccaaagggt cggcatcggc tggatcggtt cagcctgcgg aacatgcaac
     3001 tattgccgcg gcggcatgga aaacctctgc ccggatttca gggcgaccgg ccgcgacatt
     3061 ccgggcggct acgcacaata cacaacagtc ctcgaggatt ttgcctttcc cgttccggac
     3121 gctttttccg acgcgcaggc cgcgccgctt ttgtgcgcgg gcgccatcgg ctggcggtcg
     3181 cttcgcctgg ccggccttgc cgacggagag cccctggggc tcacgggatt cggcgcttcg
     3241 gcccacctgg ttctgcagct tgcccgctat ctctatcccc gttccaaaat atgtgttttt
     3301 gcccgctccg aacgcgaacg caactttgcc ctttctctgg gcgcgggctg ggcgggggaa
     3361 cccgctgaaa aaagcccgga gaaacttgcc gccgtcatcg acacgacgcc cgcctggaag
     3421 ccggtggtcg aagcactggc caatctcgca cccggcggac ggctggtgat caacgccatc
     3481 cgcaaggagg cgtcggaccg ggaccagctc ctgcgcctgg agtatccgct tcatctgtgg
     3541 caggaaaagg aaatcaaaag cgtggccaac gtggcgcgcc gggacattgc ggaattcctc
     3601 gatcttgccg cgaaaatacc gatcctcccc gatgtggagg aatatgcttt tttcgacgcc
     3661 aaccgggcgc ttgccgaact caaggccggc caaatccgcg gcgcaaaagt attgaaaata
     3721 ggcaatgggc tataggccgg aggcgatagg taaaaaagag agaaaaacta aggacggcaa
     3781 acgacgctcc catagccccc ggcctatcgc ctattttcct tccgcccgcc agccggccac
     3841 atactcccat gattttatcc cggcaggcgg ttcttcctgt cgccatattc ccgccagccc
     3901 cagttcccgg acggtcaggt caaagtggca aagcgcgatg cccatgtcga tttcctgcag
     3961 ggaaacatcc cgcaggtgac ggtatcccgc gctgcgcgac aggtaaaatg aaaaggcgcc
     4021 ctgcttcgga tcacagatga tccgccaggg ctgcttgttg gaggctgagg ggcccagacg
     4081 gacgttttcc agcgcctcgg cgtaagggcc cgccgcctcg acagggagcg gagtcgcggc
     4141 gttgccgcga aagaagagtt ccggccaggg caggcggtgg tcggagcctg caaagccgcg
     4201 caacaatcgc tccgtcaggc ttcggcgccc ggccgcatag cccacgggac tgacggtcgg
     4261 catcagttcg tcgcgccgca tgcccgccgc tcgaccgaag gcgctgatcg cgaaggttcc
     4321 gcccagccag caggtgctaa gccccagttc ggtggccttc aggatcagtt tttccttgca
     4381 gtatccatag tcaacggtgg ccatcggcgc ggggcggatc atccccgcaa tgtagagccg
     4441 ggcgttttga atcacgccgt acgtgcccag ctttttccag gcgtctcgcg gcagttcgtc
     4501 catctccagc aggacaaagc gcggcgtgtt tccgaacaat ccccgcgcgg acgcttcgac
     4561 ggcctgccgg aacgtccgaa gaaggttgtc ggccaggggc ttgttttcgt aagaccggca
     4621 ggaaaaacgt ctgcggatga tgtcggaaac aggggtcata tcgcctcccg caaattttta
     4681 ttgtcaaatc tctcgcgatt ccttatatac tatccactca aaaatggtaa acgacttttc
     4741 ggttcggagg aataacgaga ctatgaaact cgccaccgtc gagcaaatgc gtgaaatgga
     4801 tcgccaggcg attgaaaaac tggggattgc ggaagaaatg ctcatggaaa acgcggcgca
     4861 cgcggcggtt tcgctgctgc agaatgcttt cggcatccgg gacagaaact ttgtgatttt
     4921 ctgcggcatt ggcaacaacg gaggcgacgg gctggccgtg gcccggctga tcaagtccgc
     4981 cggaggccgc gcaaaagtat ttctgacggc ttctcccaaa aaatacaccg gggccgccaa
     5041 actcaactat gacatcttat gcaacctgga cgcggacgtt caactgcttt ccagagccca
     5101 ggaggcgcgc gttgacttgc tggccgccga cgttgtggtg gacgcgattt tcggcacggg
     5161 gctggaccgc gacgtccggg ggctggccgc cgatgtgatt gcgctcgtca acgcctgcgg
     5221 ccggccggtg gtgagcctgg acatcccctc cggcatctcc ggcaacagcg gacaggtgat
     5281 gggcgcggcg gttcgcgcga cccacacggt gacgttcggt ttgccgaagc tcgggaacct
     5341 tttgtacccc gggtacgaac attgcggggc gctttcggtg acgcatattt cgttcccgcc
     5401 gtctctttat cagggggacg cgctgacggt ggagaccaac ggcacggtgg cgcctctgcc
     5461 cccgcgtccc gcccaggcct ataaaagctc catgggagac gttctgttta tcgccggagg
     5521 cggcaactac ttcggcgcgc cgtatttttc cgcgatgtcc tttttgaaat cgggcggagg
     5581 gtacgcgagg ctggcgtcgc ccgcgtcgat cattcccttt gtcgcccagt ccggacgcga
     5641 aatcgtttat ctgccgcagc gggaaacggc tctgtttggc ctttcgctca aagccaaacc
     5701 ggatttgctg gcgctggcgg aaaaggccga tatggtggtg atgggccccg gcctttcgct
     5761 gcaggaggaa acgaccaagc tcatccggga actcgccgcc gtcatttccc ggccgctttt
     5821 gatcgacggg gacggtctga cggccatcgc cgagcggccg gaaattctgc tcaaaaggaa
     5881 ggcggaaacg attctgacgc cgcatgcggg cgaaatggcg cgtctgacgc gaaaaacggt
     5941 cgcctgcgtg aatgccgaca agatcggcgt tttgcgggaa gcggcgaaga agctgaacgc
     6001 cacggtggtt ttcaaaggcc cgcactcgct cgtcggcctg acggacggcc gggtgtatgt
     6061 caatttaagc ggaaatccgg gcatggccac cgccggaagc ggcgatgtcc tgaccggctg
     6121 catcgcggcc atgcacggtc tgggattaca gcctgcgctg gccacccgca agggcgtttt
     6181 cctgcacggc tgcgccgggg atcttgccgc ggcggaaaaa ggtcaggacg gcatgaccgc
     6241 gacggatatt ctggaacacc tgccgcaggc gctgaaaaat gatcgtgagg gcgccgccga
     6301 cgaaaagcat tacgaaccgc cggttctgtt gtagaggcac ggcgttgtga cggaccgcac
     6361 cgcagcgggc cgcacttcgt gattgacttt ccttcagggc cgcgcctata atttctccgc
     6421 aaaaatccga aagacaggag aaatatcatg gccggaatac cccatctcca gtggtcccag
     6481 agtctcagtg ttcgggtgga cggcattgac gcccagcaca aaaaactttt tgaaattacc
     6541 aatcatttga tcgatgtttt tgaagccgga tccggcgact ttctgacggt gatcagtgag
     6601 ctggtcgaat acaccaccgt tcacttccat gacgagcaga tggtgatgat gaatgcgaaa
     6661 tttccgggcc tcgcggcgca taccgcggag cacgacaaat tcatcgccaa agtcgaggaa
     6721 tttctacagg cctacggcga aggcaacgag gacgtggggt ccaaaatgct cgtctttttg
     6781 agagactggc tttttgccca tacgtcggga ttggacatgg aatatgcgca atttctggcg
     6841 aaaaactccg caaaaaccaa ggctttctga accccgtttg tccatcatga taaggaaggt
     6901 tgactatggc tgacatgtcc aaagttgccg gattgcagga tgtcttaagg cagcagaagg
     6961 gattgcagag catcaccagc cgcatcaatg ccgcgaccgg catccggcag atgattgctg
     7021 agatttgtcc tctgctgatc gacctgttcc gggtcgaagc ggcgcatgtg ttcgttcgcg
     7081 aaaaaaaccg gcaagacgtt tatacgttta cgggtcagac cggggatgta aaaacggttc
     7141 tgggccctca aacgcttccc ggatatatcg cgaacgaagg gaaaatgatt catatcagtg
     7201 atttcaataa atctgaccaa atcgaggcct accgggattt tcccgtcagc ggaggcgggg
     7261 acagccgttt cggcctccct gtgcggcaga ttctggccat gccgattttt tatggcggcg
     7321 aaacgctcgg cgccgttgag gttctcaaca aaaaggaaga cggacccttc ggggaaaaag
     7381 agccggcgct tttgcagaat atttccgagc tgctcggtgt ggccctctac aattttctgc
     7441 tggttgatcc caaggcaaaa agggtgaaag gcgcctatct ggtgtctcag ggtctgattt
     7501 tgcaggaaga aatggaccgg gccaaagaag aatcccgggt gaccaaagag cctctccagg
     7561 atgtgctggt gaaaaaattc aaaatcagca aggaagatgt cggccgggcc tgtgaatttt
     7621 tctggcagtg caaatttgtt tctttcgaca agaaaacgcc cattcccggc gagcttttga
     7681 aaaacctgaa aagagactac ctgatccggg aagtctgggt gcctctggaa agaaccgaca
     7741 agggcgttcg cgtgatcatg gatgatcctc aggacattct ccggcgcgac gccgttgaaa
     7801 agtcgctcaa ggcaaaatcc gtgcagtatg acgttgcact gaaggaggat attctccagt
     7861 tcattgatct gttctttcag gagagcgccg gggaatcaac cttttcggag ctgctggata
     7921 aactcgaacc gggcgagagc gaaaggaaag aagaaacgga agacgttgac gagtccgaca
     7981 gcgccatcat ccaactggtc aacaaaatca tcagcgacgc gttcaaccgc cgggcgtcgg
     8041 atattcatat cgaaccgaat gtcatcgaca agaacgtgct ggtgcggttc cgggtggacg
     8101 gggactgcgc gctttatcaa accgtgccct acagctaccg gtcggcgctg atttcccgca
     8161 ttaaaatcat ggcgaatctc gatatcaccg tgaaacggat tccgcaggac ggaaagatca
     8221 agatcaagcg gtccggcgcg gaggaaatcg aactgcgcgt cgtgacgatt ccgacacagg
     8281 gcggcgtgga ggatgtggtc atgcgcatcc tggccaaagg cgacgccctg ccgctctccg
     8341 ccatgcggat gtcgaacaga aactatcagg acattctgaa aatctgcgag cagccctacg
     8401 ggatgattct ggtggtcggc ccgaccggtt cggggaaaac gaccacgctg cacgctattt
     8461 tgaatcacat caacaagccg gaccggaaaa tctggaccgc cgaagatccc gtggaaatca
     8521 cccagcgggg cttgcggcag gtgcaggtgc agccgaaaat cggatttgat tttgcggcgg
     8581 cgatgcgggc gtttcttcgc gccgacccgg atgtgatcat ggtcggcgaa atgcgggatt
     8641 ttgagacggc caagatcggc gtcgaggcgt cgctgacggg gcatctggtg ttgagcacgc
     8701 tgcataccaa cagcgccccg gagacgattt cccggcttct ggacatgggg atcgacccgc
     8761 tcaatttcgc ggattccctg ctggcggtgc tggcgcagcg gctggtccgg acgctttgcg
     8821 gcaattgcaa agaggcctac catccgaccc gggaagaata cgatcagatc gcgcaaagct
     8881 tcggcgccga atattttgca aagcagaacc ttccctattc cgacgacctg acgctttatc
     8941 gtcccaaagg gtgcgacgcg tgcgaccaga cgggatacaa gggacgcatc ggcatccacg
     9001 aactgctcct gaattcaccg gaaatacgga agctggccat tgaccggcag gagagcatcg
     9061 aaacgctccg gaaagtcgcc atgaaccagg ggatgttgac cctttaccag gacggcattt
     9121 tgaaagcgtt tcagggcctg acggatatca aacaggtgca tcgggtctgc gtgacgtcga
     9181 aatagagccg cgatgcgggt tttgtctttt ccgccgccgc cggcatgagc gtcagatttt
     9241 cctcgacaaa atgtagccgg ccacggccgt caggtgttct ttttcgggac tggcgggaaa
     9301 aacgtccaaa ccgcttcggg cctgcgtgat gaattgctcc gcgcgctgga gcgaataatg
     9361 aatgccgccg gattgttgaa tcacctcaaa aatatttcgg atggcttcgg aaggcaaatc
     9421 ttttttctca atcgcggttt tgatcttttc gcgccggtcc ggcgggcagt gagacagcgt
     9481 gtaaatgagc ggcagcgtca ttttgccttc ttccaggtcc ttgccgatgg atttgccgaa
     9541 atcctcttcg cgggccatgt aatccagcgt atcgtccgtg atttgaaacg ccatgccgat
     9601 gttgtagccg tattgcccga gggcgcgggc ctgcgactcc ggcgcgccgc ccagcgcggc
     9661 gccgctttca caggcggagg agatcagcac ggcggttttc ttctccacga tgctcagata
     9721 ctcctcttcg gtgaggctgg tgtctccgca cttcatcaac tggaacactt cgccttccga
     9781 catgacgttg gtcatcttcg acatgagctg gacgacccgg atgtcgccga tgtcggccag
     9841 aaggctgaaa gctttggaat acagaaaatc gccgaccaga acgctggcgg cgttgcccca
     9901 gacgcggttg gccgaggttt tcccccggcg gaggaggccc tcatcgatga cgtcgtcgtg
     9961 caggaggctg gccgtgtgaa tgaattcaat ggaggccgcc agcggaaagc ggcgggggcc
    10021 gtcatagccg caaagcctcg cgcagatcag atgcagaagc ggccggaatc gtttgccgcc
    10081 gctgtcgatc aggtgatgcg cgacgtcggg aatcagccgg acgtcggacg tgatgtaccg
    10141 gtccaggtgg gtttcgacct tatgcatttc gtttttatag agggagaaaa tatccgatag
    10201 atccatgata aacgcttgcc ttgtttgttg gtgagctgac tatagtggtt tttttctgta
    10261 attgtcaaac aggatttcac cgaaaagatc gtaatggtcg gcgccggtga tcctgcagcg
    10321 gacgaaatcg ccgatggcgg ccgggccttt tttcaggtag gtgactccgt cgacgtccgg
    10381 cgcctgccgg cggcagcgcc cgacccgcgc gaaatcggga cggtcgctga ttccctccat
    10441 caatacttcc tggacggagc caatcagcgt ttggttcacg gcgtaggaaa cgtccgcctg
    10501 ttcgctcatg atcgcgtcgt gtcttcgatc cttttctttt tcagagatcc gcgacggaag
    10561 atccgcggcg ggcgtgcctt cttcccgcga ataggtgaaa acgcccaggt ggtcaaatcg
    10621 cgcttctgcc acgaaatcga gcagccgctc aaaccgcttt tgcgtttctc ccggaaaccc
    10681 cacgatgaga gaggtgcgaa gcgcaaggtc gggcatcagg cttctcgcct gacgaagtat
    10741 ttcccgaatc atctccgatg aaacccggcg gttcatggcg tgaaggatcg cgtcgtcaat
    10801 atgctgaacc ggcagatcca gatagcggca gatgttttcc cggccggcca tcgcctccag
    10861 aacatcgggc gtgatgtgcg ccggatgggc gtacagcagg cgcagccagc gcagaccggg
    10921 aatcgccgac aaatcggaca gcaggccgga aagcgtcggg cggtttttca agtcgctccc
    10981 gtaggcggtg gtgtcctggc cgatcagaat gatttcccgg atgccccggc cgaccaggat
    11041 ttccgcttcc cgcagaatat cgtccggcct gcggctgcgc gccctgccgc ggatggaggg
    11101 gatcgcgcag taggcgcagc ggttggaaca gccgtccgat attttcaaat aagcggtggc
    11161 ggcgccggag ggcagcagcc gttcgtgccg cgacgtcatc agaaaatccg gcttggagac
    11221 aatcgccttc cgccgggtcc cgcctgcgtc gagccttcgc aaatgttcgg caatgcgtcc
    11281 gacttctccc gtgccgatcc acaggtccgc ttcgggaatt tccgaagaga gcgccttgcc
    11341 gtagcgctgg gccaggcagc cggccaccac cagcttcagg tcacggccgc gctgtttttt
    11401 gatttgcgcc agggtcagga tcgcttccag cgattcttct ttggccggcc cgataaagcc
    11461 gcaggtattg atgaggacga catccgcgtc ctgcgggcgc tccgccatcg tcattccgga
    11521 ggcccgggcc agcccgccca tgacttccga atcaataaga ttcttggagc agcccaggga
    11581 gaggatgtgc agttttccgg aaagcaattt tttcactgcg ggagtttccg aatcagaact
    11641 tttcggggtt tcgcgccgtc ggaggggccg acgatgcctt cgcgctccat ctgttcgatc
    11701 atgcgcgcgg cgcggttgta gccgattttc atgtagcgct gcaccagcga gatggaggcc
    11761 tgccccagtt cgccgacgag ggccacggcc tcgtcgtatt tttcatcgta gccgtcttca
    11821 tcctgccggg tttggccgga ttccagttca aatttttcaa tcgaggcgtc gtagatcggc
    11881 tgggcctgca ttttgatgaa ttcgacgatg cgcgatatct ccgcgtccga cagatacgcg
    11941 ccgtgaatgc gggtgagctt ggaggtgccg ggcgggatga agagcatatc gcccgcgccc
    12001 aggagctgct ccgctcccgg ctgatcgatg atggtgcggg aatcgatttt ggaggagact
    12061 ttgaacgaaa tgcgcgtggg gaaattggcc ttgatcagtc cggtgatgac gtccaccgag
    12121 ggacgctgcg tcgccagaat gaggtgaatg cccgccgcgc gcgccatctg cgccagcctc
    12181 gtcagcgatt cttccacgtc tctgggggcg accatcatca ggtcggccag ctcgtcgatc
    12241 acgatgacaa tgtagggaag cgtgacgtgc tgaaacggcg cgtcgctttc ggccgaatcc
    12301 tccgccgggt ccctgtcggg ccggggctcc ggtttcggct ccttggccgc cgttctggct
    12361 tttccgcggg tgatgatgat ttcgttataa gcgtcgatgc tttttacgcc cagttcgctg
    12421 atgatttgat agcggcgctc catttcttcc accgtccagc gcagcacctg cgaggctttt
    12481 ttcgggtcca cgacgaccgg atgcatcaga tgcggaatgc cttcgtagga cgacagttcc
    12541 agccgcttgg gatccaccat gagaaatttg atgttgtccg gcgtcgcttt gaagagaatg
    12601 ctgcagatca tggcgttgag cgcgacgctt ttcccggagc cggtggttcc ggcaatgagc
    12661 aggtgcggca ttctggccag atcggcaatg accggcgcgc cgacaaagtc aacgcccagc
    12721 gcgatgggca gacgcaggga ggattcggta aaggcgtcgt tgtcgagaac atccttgaga
    12781 aacaccggct ctctcttgag gttggggatt tctatgccga tgacggattt gccgggaatc
    12841 ggcgccagaa tgcggatgct gggcgccatg agcgccagcg ccaggtcgtc ggtcaggctg
    12901 gtgatgcggt tgatcttgac gcccgcagcc ggttccagct catacatggt gatgaccggg
    12961 ccgggcagaa cctccaccac gcgcccctcg acgccgaagt cggcgagctt tttctccaga
    13021 atgcgcgagt tggtgatcaa atggtcgcgt ttgacgcggg cgtctttttg cggcggctcg
    13081 ctgagcagcg aaaacggcgg cagctgaaag gcgccgtcgg atggaagttt tgcaaaatca
    13141 aaatgggtct gctcgacttt tttcgtcttt gcttttttcg gcggggcttt cgccgcggcg
    13201 tcttcgatga cgggcggcgg acttttttcg gcttttgccc tggcgaacca accggtgaag
    13261 aaggagacgg cccggccttt gaaggcggaa agcccctccc ggacggattg caggaatttt
    13321 tcggttgtcg aaacgagaga aaaatcgatg ataaaaaaga gcgaaaccat catccaggcg
    13381 atgagcagaa tgaaggttcc cgccggattg aaatagccct gcagaaaccg gacgaggccc
    13441 gcgccgagaa tgcctcccga cggaaaggtg gctccgtaca ccgtgacgcc gccccggatc
    13501 agggcgtcgg aaagcgcggc gagcgagagc gtcagggaca ggaagccgaa cacgcgaggc
    13561 gtcttcaggg aaaactccgg acgccggaaa aattgaaacg ccagcgcaag cgaagaggcc
    13621 ggcagaagaa acgaggcgag gcccagaaga aaaatcatcc agcgggctgt ataggcacca
    13681 acaattcccg tccaattgtg tgttgatgag ggctctccta cggatttcaa caggggcgcg
    13741 ggatcctccg gcgaaaagga aaaaaggcat aagaagagaa acagcgacag cgcgaggcag
    13801 aggacgccct tgatttcccg aaatcgcgca ggcagaggat gctgggaggt ttcttccatt
    13861 agagcgccgt ctttgtgtcc gtctggacgt ctttgatgcc ggccttgccg gcgtcgcggt
    13921 aggcgtcgta attgcgaata atgacctgcc ggactctgtc gatcagttca tgccggtttt
    13981 ccagcgagta tccggccacc ggaatcggtt tgtcgatgat cagcttgact ttccccggtt
    14041 tgattttcag cgaacgcttg ggcatgattt cgccgctgcc gatgatgctg atcggaacaa
    14101 tcggcgcgcc cgcctgaatg gccaggtaaa aggtgccctg cttgaaagcc ttgatttctc
    14161 cgtcgcggct tctcgtgcct tcggggaagg tcatgaccga cttgccttcg cggatgcgca
    14221 gcgccgccag ctccaggctc cgcagggctt tttcgtggtt ctgccggtcg atttcgatgt
    14281 agcccgcgct tttcatggcc tcgccgaaga cgggaatggc aaacagctct tttttggcga
    14341 tccagcgaaa ctgtcccgga acgtgggcca gcgtgatgag aatgtcaaaa tcgctctggt
    14401 ggttggccat cagcacctgc ggtttgcccc gcaggaggtt ttcccggccg atcacccgca
    14461 ctttcgttcc gcagatcgcc agcagaatcc ggctccagat gttggccacc ttgtggacgt
    14521 tgttttccgc gttggcaaaa aaggaaaaga ccaccgccca gaatgaaaag aaagcggtaa
    14581 tggccacgcc tatggagact aaaacagctg aacgaatcat cggtattttt ccctgcttac
    14641 gcgccggaat gctgaaaatt ttactcggca ttttttactt gataaacccg ttgaagtcaa
    14701 cagcgaatcg cgtttttttc ttcctgcctg tgtccgcgtg aggaaagaaa atcagcgggt
    14761 ttgcggtgac tcccggtcat gttgttattc aggcagagta agggattgcg gctttttaaa
    14821 aatttattaa aaatctcgaa aaaaagattt tctgcttgac aaatgatggt cgctcatcta
    14881 aacagcctgc ctaagcaaaa gacattcaag agcaacgcca atggaacgcg cctgaacttt
    14941 ccgtgtttgg atgcagggga acgggacgcg tcgtccggat gatggagtat ccatggaact
    15001 atttctgcaa caggttttta acgggatcat gttggggagc acttatgcca ttgtcgcgct
    15061 ggggctgacg atggtctttg gtattctgca cgtgccgaac tttgcgcacg gtcagatgta
    15121 catgctgggt gcttatgtct gcttcttttt aatcaccatt ttcggttttg cctactggcc
    15181 cgcgatggcg atgtcggccg ttgtccttgc gctggggggc gtcattcttg aggtcctggt
    15241 cttcaaaccg gtgcgcaacg ccggaggcat tgctcccttt atttcagcgc tgggcgcgct
    15301 gatcatcatc gaaaatgcgg tggttgtttt gtggggaccg gaaggccacc ggattccgaa
    15361 tccgtatccc ggcatcatcg aagtcttcgg catcaccatg tccacgcagc gcctgctcgt
    15421 gatcggcgtc gcctttgccc tgatcgtgct tctgcagatt ttcattaaaa aaacaacgtt
    15481 gggcagcacc atcgaagccg tggcgcaaaa taaggaaggc gccacgctgg tcggtatcaa
    15541 cgtcaaccgc gtctccgctt tgacctttgc catatccacc ggtctggccg cggtcgccgc
    15601 ctgcctggtg gcgcccattt ttatgatatc tcccagcatg ggaatgatgg tcgggatgaa
    15661 agcctttgtc attgtcatcc tgggcggttt gggcagcatc ccgggggcga ttgtgggcgg
    15721 tttgatcctg ggcctgattg aggcgatcgg cggcgggtat ttttcggcgg cttacaagga
    15781 tgtgttcggt ttcggcgctc tggtgctgat cctcgccata aggccaacgg gcttgtttgg
    15841 gaaggagatt aagggcagat gatgaagtgg ctgaagacaa attggtttta tctggtcgtt
    15901 ctggtaattg ccctggcgct tcctctggtc ataacgaacc ggtactactc gcaagtcatc
    15961 acgatgagcc tgcttttcgc gatcggcgcc ttgagtctta acctcatcct gggatttacc
    16021 ggtcaaatgt cgcttgccca cggggctttt ttcgccatcg gagcatacgg cgtcgccatt
    16081 cttacctata cgctgggatg gaatttctgg cttgcccttc cgtcctcggc gctgcttgcc
    16141 gcattcatcg gctttctggt cggcatgccg gcgcttcgca cgcggggatc ctacttcgcc
    16201 attacgacca tgtgtctggg cgagattatc tatctgatcg ccggcaactg gatggaactg
    16261 acggggggcc acaacggcat cgtgggcatt ccggtgccca gtccgattgc cgttcccggc
    16321 gtgcttgaaa ttactttcca gtcgcaggct tctcaatatt atctggtgct gtttttcctg
    16381 ctcttaaccg tttttgtcat gcatcgcctg acgaagtctt tgcgcggcat gacttttttg
    16441 gcaacggcgc tgaatgaaga tctttccgaa gcggtgggga tcaatacgtt ccgcaccaaa
    16501 ctgctttcct tcattgtcgc caacttcttt gccgcgctgg ccggaggcat ttacgccagc
    16561 ctgatcggca gtatttctcc gtcggtggcg tccctcacca tgactttcaa tttcctgatg
    16621 ttcgttctgc tgggaggcat taccacgctg gccggaccca tcatcggcgc ttttatcatt
    16681 ccgattcttc tggagtatct gcaatttctg caggactacc agatgatttt attcggtttc
    16741 ctgctgattg tcgtgattat ttacttcccg acgggtttta tgggattgta tgcaaaagtg
    16801 cgtgacaaga tcaagaaatc aagggtgggg gcgtaacgtg ctgctgaaag gtgaaaatct
    16861 ggtaaaaagg tttggcggca cgattgccgt caatgaggtc agctttgaag tccatgaagg
    16921 agaaatttta tccatcatcg gccccaacgg cgccggcaaa tccgttctct tcagtctgat
    16981 cagcggagcg ggaaagccga cgtccggcag catcaacttc gacggacatg agattgccgg
    17041 cttgagcgcc agccagatct cccgcaaggg aattgcaaga accttccaac tgacggctct
    17101 ttttgaaaag ctgccggtcg ttgcgaatct ggccctgggg tattacaaat gcacgaccag
    17161 cggtttctgg ggagggattc tgcacacgcc ccgatggcag cgggatcgaa agaagaccga
    17221 agaaaaggtg ctcgaaacgc tcgaatttgt cggcctgggc gccaaagcct tcgacatggc
    17281 gtccaccatt tcccaggggg aacagcggtt gctttccatc ggcattgccc tgatcagcga
    17341 tcccaaactg atcctctttg atgagccgac aggcggattg attcaggaag acactgcgat
    17401 gatcaccgag ttgatccgta agatcaacaa aagaggcacc acggtttgtc tgattgagca
    17461 taaaatgcgg atggtcatgg atttggctga ccgaatcgtt gttttaaact acggcaagaa
    17521 aattgccgaa gggaagccgg cggatgtcgc cgcggacccg caagtaatcg aagcctactt
    17581 aggaagaaaa aagaatgctt aaccttacga atgtcacgac gatgattggc gaacatcagg
    17641 tgatccacga tgtttcgctg catattgccc agggagactt tgtggcgctg ttgggcggaa
    17701 acggcgccgg aaaaaccagt ttgtttcgca ccattgtcgg ggtgctgccg ccggcaaagg
    17761 gcaagattga attcaaaggc gaggagatca gccacatcgc cgcgcataaa atcgtgggca
    17821 agggcatcgc gctttgtccg gaaggaaggc agctctttcc gcagctttcg gtcaagaaaa
    17881 atctgatgct gggcgcttat caccgaaaag acaagaaagg catcgaagaa tcgctggagc
    17941 aggtttacgc gctcttcccg cgcctcaagg aaagatacaa gcagatggcg ggaacgtttt
    18001 ccggcggcga acagcagatg ctggccatcg gccgggccat catgtccaaa ccggaacttc
    18061 tcctgctgga tgaaccgtcc atgggcctgg cgccgctggt ggtggaaggc atcgccgacg
    18121 cgattacgca gctcaacaag atgggcatta cggttttcct gtccgagcag aacgctcaga
    18181 tcgcgctgat gatcacgcgc cacggctatg tgatggaaaa cggccatctc gttctcgaag
    18241 gattgagcaa ggacctgatc accaatgaaa aggtcaggaa ggcttatctg ggaacctgaa
    18301 atatttttcg gccgccggat cggaacgaaa tggcgtcatc caaagagctc ttaccccttc
    18361 ggggggagag ctctttttct ttcatgcgcg gagcgctttc ggagacctat gcaaaagaat
    18421 taatctgttt gacacgaaca tgcttatttg ttaaaaatat cacaacttgc aaccttgtga
    18481 ggttttgtga agaaccgatc aaaatggttt tccgcgctga tgatttccct tcttctgttg
    18541 tatggccagg ggagttttgc ccaggggccg cgggcgaaaa aagccgcttc ggccaaagtc
    18601 gtccgggttt ccggcgcaca ggcctgggaa tgcgcggcct gccatcgcgc tcagaatgtt
    18661 cttccgaaac aacacgtgga tattcaggaa atgccgtatg tcggctgcgt ggtttgccac
    18721 ctttccgaag aaggcttcgc gggcgccctg aagggaaaaa tgccgggcag ccacctgcat
    18781 gccttccgga acgttccctg cgccggctgc cacggccagg agaaaaaacc ggccccggtc
    18841 gccaaaagcc agtgtctggt ctgccacggc agcgcggcaa aagttgccct gaaaactcaa
    18901 aacaccaaac cgaacaatcc gcacgactcg ccccactacg ggacgcagct ggactgtaat
    18961 ttttgccacc gccagcatgc aaaatcggag gattattgcg cgcagtgcca caaatttaat
    19021 tttgttgtgc cgtaagatca acctgcccgg gcaggcaaag gaaaaataac catgggatgt
    19081 ccggaaaatt catcatggac aggctgtctt aaggatgccg gccggcgcgt cttctccggc
    19141 ggcccggtgt atctgttttt tctggggctc tttgccgcgt tgctggtcgc cggggttgcc
    19201 gcgggaattc actccgttta tatcgtggga agccgccacg cctacggcac cacccgggag
    19261 attccgctcg ccatgctcat cgccacctat accttttttg tcatcacgtc gacagggctg
    19321 tgcctggtgt cctccatcgg acacatcttc ggcgtggagc gcttcatgcc gatcgcaaag
    19381 cgggcggtgc tgctgtccgt catcaccatc atgtcgggat tcctcgtcat cggcctggag
    19441 ctggagaatc cgctccggct ggccgtttac ctgttttgct cgccgaattt cacctccaat
    19501 atctggtgga tgggggttct ctacgccttc gaggtggtcc tgctgatcct tgaatttatc
    19561 ttcctcctgc tggacaaaca caaagcgtcg gtggcggcgg gtctgctggg gatcctcttc
    19621 ggcattgccg ccatcagcaa tctgggcggc gtctttgcca tgctcaacgg acgggaattc
    19681 tggtacggcc cctatctgcc cattttcttc attgcctccg ccctgctgat gggctgcgcc
    19741 gccgtgattt ttttcacggt cctcgcctac cgcgtccatc gcgaaccgc
//
LOCUS       DAOSRM010000039        18675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3128_length_18675_cov_104.973469, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000039 DAOSRM010000000
VERSION     DAOSRM010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 18675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18675
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3128_length_18675_cov_104.973469"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..371
                     /locus_tag="PLA58_05435"
     CDS             90..371
                     /locus_tag="PLA58_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOR62271.1"
                     /translation="MPNAPKIIPISDLRQNASEVVKSVSSSREPIFITQRGRAAAVMV
                     SMEVYKNAQHEIDILHLLARGEKEIAAGTGYELADVLKEADHYLEAAKI"
     gene            368..664
                     /locus_tag="PLA58_05440"
     CDS             368..664
                     /locus_tag="PLA58_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOR62272.1"
                     /translation="MKILFTPTGRRQFLGAISYIHRDKPSAAVSFRQKAEKLLTRLKK
                     FPESGRPLPEFPELSFREVIVSPYRFFYKVKDNNVWIVAVWHGAQLPDEPEKIG"
     gene            793..984
                     /locus_tag="PLA58_05445"
     CDS             793..984
                     /locus_tag="PLA58_05445"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOR62273.1"
                     /translation="MRTTLNIEDNLIDKATKITGIKEKTTLVKLGLEALIARESARRL
                     AKLGGTQKQLQEIPRRKGA"
     gene            991..1353
                     /locus_tag="PLA58_05450"
     CDS             991..1353
                     /locus_tag="PLA58_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOR62274.1"
                     /translation="MVLVDTSVWVSHLRDGNTEMAALLNNGRVLCHPLIVGELACGNL
                     KDRAVILSFLKLLPMCIEAEHEEVLSFIENNRLMGKGIGYVDAQLVASSVLTGVPIWT
                     LDKKLAQVADSLHVKYKN"
     gene            1484..1726
                     /locus_tag="PLA58_05455"
     CDS             1484..1726
                     /locus_tag="PLA58_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOR62275.1"
                     /translation="MTTFTASEARKRLYNLVDEVKDSHVPVQIVGKRNSAILVSEEDW
                     RAMEETLYLASIPGMRESIKKGLKTPVEKCKEEPGW"
     gene            1723..1986
                     /locus_tag="PLA58_05460"
     CDS             1723..1986
                     /locus_tag="PLA58_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOR62276.1"
                     /translation="MSWKLVFTKQAQKDAKRIAQSGLKPQASRLLDVLKKDPFQNPPP
                     YEKLMGDLSGAYSRRINIQHRLVYQVLKDVRTVKVIRMWTHYE"
     gene            complement(2392..3399)
                     /locus_tag="PLA58_05465"
     CDS             complement(2392..3399)
                     /locus_tag="PLA58_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOR62277.1"
                     /translation="MNKKFLFGILLGVVLIYFSLRGINFNDTLTSLKKIHPGWAALSL
                     FFIVFMQALRSYRWGVILEPMEKISQGTLFAVTSVGFLAIAAIPARIGELARPYLIAQ
                     KSAVKMSSALGTIVVERVLDSLAVLTITIAVLMAQNDLPRWMVTSGILFFLGTLMMIS
                     FVIGLVWRRETAVSIIDRILRRLPGALAQKVNPLIHHFIDGFLVITDVKRLLYLLVLS
                     AVVWLVDVAAIYTLLIAFGFDLPALAAFVVMVILIAGIAIPTAPGFIGNWHYACILGL
                     SLFGIAKPEALSFALVYHFLSMMVVVVLGAVFLPFNKFSLSEMTKQMKKAEDRRPKTE
                     G"
     gene            complement(3440..3841)
                     /locus_tag="PLA58_05470"
     CDS             complement(3440..3841)
                     /locus_tag="PLA58_05470"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HOR62278.1"
                     /translation="MKSKIGRLVVCAFALMSLAGCGALVAGGAVVGAGAGTYLYANGE
                     LKTDYAASFDSVWSACERTIAQMRGTQVQPVREISKGTISAIINDENVKIEIAYRAKN
                     QTTVGVRVGLMGNKLSSQVIHDKIADQLAKR"
     gene            complement(3922..4980)
                     /gene="dnaJ"
                     /locus_tag="PLA58_05475"
     CDS             complement(3922..4980)
                     /gene="dnaJ"
                     /locus_tag="PLA58_05475"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOR62279.1"
                     /translation="MTKRDYYEILGVGKTASEEELKKCYRKIAMQFHPDKNPGNKQAE
                     ERFKEAAEAYEVLSDRQKREIYDAYGHAGLSNTGFQGFSGFDDVFSGFGDIFEDVFGF
                     GRGRGRGRTRPAARAGADLRYDLKISFLDAAFGLTTTIDLEKLHPCQACGGTGSAPGT
                     TPSTCPTCNGRGQVVQSSGFFTISSTCPHCNGHGKVIAKACQTCRGTGREKQRKTVEL
                     KIPAGVETGSRLRLRGEGEAGDQGGPNGDLYVFLQVEDHEFFVRSEDDIVCRVPISFV
                     QASLGATIEVPTLKETEKIKIPRGTQTGQIFRLKGKGIPHLQGYGRGDQIIEVFVKIP
                     TELTRKQEDLLREFEKAR"
     gene            complement(4977..5375)
                     /locus_tag="PLA58_05480"
     CDS             complement(4977..5375)
                     /locus_tag="PLA58_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62280.1"
                     /translation="MIARKLPDYRLKQKILYIDKTSPESLISYGDMYWEAGAFSDALD
                     FYARASHTAGIEKIRKQALEIGDVFLFQGAAKAQGLELQDADWENIAQTAVRLKKFSF
                     AGQALKKIVDTGRANALATMIKAEEAKQSA"
     gene            complement(5459..6340)
                     /locus_tag="PLA58_05485"
     CDS             complement(5459..6340)
                     /locus_tag="PLA58_05485"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOR62281.1"
                     /translation="MYKTIAQTHFPKLKFLTRGKVRDLYTVKDYLLLVATDRISAFDV
                     IMPNPIPGKGAILNKMSLFWFQKMEDIIGNHIVSADAADFPAECEPYAEVLQGRSMLV
                     RKATPLPVECIVRGYLAGSGWQEYRRDQSVSGIQLPGDLKESCRLPQPVFTPTTKAPE
                     GEHDAPITRSEMEDLISPEITEQVIQKSLAIYERAAAIALKAGIIIADTKMEFGLIGD
                     QLILIDELLTPDSSRFWPADDYEEGRPQKSFDKQFLRDYLLSIAWNQKPPAPELPPDI
                     IEKTRSKYEEAFSRLVK"
     gene            complement(6357..6986)
                     /locus_tag="PLA58_05490"
     CDS             complement(6357..6986)
                     /locus_tag="PLA58_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR62282.1"
                     /translation="MFIQQMQVGHMAVFAYLVGDPVTGDALVIDPADNVHEILALAAK
                     NKLRINSIVNTHGHVDHIGGNAQMKKLTGARIIIHEDDAFMLTSTPPAMLRMFGAAQS
                     PPADLLVSDGQIISAGNIELKVLHTPGHSPGGMCLYTPGYVFTGDTLFVEAVGRTDLP
                     GGSWQTLYRAIKTKLFSLPDDTKVLPGHNYGRTPTSTIGHEKLHNPSVR"
     gene            complement(7048..8394)
                     /gene="rsmB"
                     /locus_tag="PLA58_05495"
     CDS             complement(7048..8394)
                     /gene="rsmB"
                     /locus_tag="PLA58_05495"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOR62283.1"
                     /translation="MQKTVRHQAVEILTRVSQSDAFAGDLLDSCLEKQNLSRTADGRL
                     LTHLVYGVLRMQGHLDWILARLYRGNYLKMEENIKNVLRTGLYQLQFSDRLPAFAVVD
                     EAVKITRRVQPAARGLVNAVLRSYLRSPGPWSFPDENKNPAEYIAAFHSHPLWLVEEW
                     LNVFGRMETEALCAANNELPPLTARANTLKSGRRELIEKLAGENFQAEETRLSPDGIR
                     LSSSSLPIQKTLFFADGLLRLQDEASQLVAYLVNPRKGQKILDACAGSGGKTTHLAAL
                     MKNEGVIVALDRDAEKLVQLQNDAARMGITIVKTARVDLERPLSAQYQNAFDTVLVDA
                     PCSGTGTLRRNPEIKWRLKPKDIHLYADLQQSILQNAGAAVSKNGFLVYCTCSLFSQE
                     NDRVVRRFLADFPQFQLLLPDSPVVQGLIDSRGLFRSYPHRHSMDGFFGAVFRRRI"
     gene            8508..10373
                     /locus_tag="PLA58_05500"
     CDS             8508..10373
                     /locus_tag="PLA58_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOR62284.1"
                     /translation="MSLNQLIDSRDVRFTLFEMLELEKLNRFDAFKDFDRSVYEDTLE
                     LAEKIAVQQFYPTNAEGDKTGLKFDPKTGEVKVPESFKKGFNAYIESGFHSLALPQEI
                     GGTGLPSSISMACAEYFNAGNTSLTMYCGSITGAIAIIAPFANKELYNMVVPKLMEGK
                     WGGTMCLTEPGAGSDVGALKTKAVKQPDGTYLITGTKIFISNGEHDMTENIIHPVLAR
                     IEGDPEGTKGISIFVVPKFLINPDGSLGERNDMICAGIEHKMGLKGNATSMLTFGDNG
                     KCVGYLLGKEREGMKIMFHLMNAARIHTGVQALGCSSAAYLHAVTYARNRVQSALITN
                     PKGGPVTIINHPDVKRMLLYMKSNVEGMSMLCLFLALHEDIMRASKDPEEVKRATGIV
                     EILTPIVKAGISDASWLVTAEAMQVYGGYGFCQEYPVEQYARDTKVFSIYEGTNAIQS
                     LDLVMRKILMNPEMYNYAQLKQWMSDTIAKAKGIVDDRYVNPVVEGLAKLEEVIEMMK
                     KQLGEMKILLLLNNATPFQQAMYPLILAWLHLWSLTLTTPKAKALVGDAKGADRAKIL
                     AENPEAAYYSGRVLSSQFYIGAEFPKFFGRIAAIVNNEGAALKVEADNFTGALKE"
     gene            10533..11684
                     /locus_tag="PLA58_05505"
     CDS             10533..11684
                     /locus_tag="PLA58_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOR62285.1"
                     /translation="MKAEDVKKIAIIGAGDMGHGIAASCLLGGYAVVLRDIEQKFVDK
                     GVAGIIKSLEKFREKGKITPDAFAHALVRLTPIVDLQEAVRDADFVIEAVPEKLELKK
                     SVFADIDKYAPKHAILASNTSNISITEIASATQRPDKVIGYHFFNPAILMKLVEVIKG
                     RGTSDESVQVGYDIAKKIKKVPVIVKKDSPGFIYNRVNEPTLVLLSKILEAGHPSPEE
                     FDAAFKPLMAMAPFELVDYVGIDVAAHGLEYFAQVLSPDYKPSEAMMSYLKINHLGKK
                     TGRGFYDWSAGRPAIDLAKATKEYDINQLIALQVNEATKLLEEGVVDDPKEIDLAMAN
                     GGGSPFGPFALAQGIGYAELVFKLGEIYKKFPLDIFKPTKTLKEGKIKL"
     assembly_gap    11857..11956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11957..12475
                     /locus_tag="PLA58_05510"
     CDS             <11957..12475
                     /locus_tag="PLA58_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOR62286.1"
                     /translation="HPGRHIPAVIRTGQGNMPVSFLWGLVPFWAKDPSIGPRLINARA
                     ETIAQKPAFQNAFRKRRCLIPADGFYEWKKDGASKKPFRFGLKSGGPFVFAGLYEQWA
                     AAGQKPLDTCAIITTSANEVVARVHDRMPVIVPKSSQELWLNPELQDPALLAGVLKPY
                     PAEEMICEPADI"
     gene            12636..13550
                     /locus_tag="PLA58_05515"
     CDS             12636..13550
                     /locus_tag="PLA58_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOR62287.1"
                     /translation="MTLKGIITVIRVPFLILALTLGILGAAVAWYESRRFGSPFHLGY
                     AALATFGLLVAHAGVNILNDYFDAKSGLDYKTLRTPFSGGSGAAPTGLLTVAQVLWLG
                     IACFVILIPIAWFFVVKAGWMLLPLLVAAAALIVFYTPLILKMGYTEWSPGLGLGILP
                     VMGAYFVHTGAYSMSAFIASMPSFFLVHNLLLINEFPDVEADITVGRRTLPIVAGKKN
                     AAYFFSLFTVLVYCWVIGAVLCGSMPAFTLLSLLTVPIAVRVIQGALRYDDRETLLKA
                     MSANVLLVLLTQALIAAGYIVSGILLIT"
     gene            13876..14949
                     /gene="hisC"
                     /locus_tag="PLA58_05520"
     CDS             13876..14949
                     /gene="hisC"
                     /locus_tag="PLA58_05520"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOR62288.1"
                     /translation="MSRFWSQTTKNIKPYVPGEQPKDRKYVKLNTNENPYPPSPRVLE
                     VIRSAADETLRLYPDPSGDALRDAIASSFGLIRENVYIGNGSDELLAFCFPAFFEPEG
                     LPILFADVTYSFYPVYARLFRIPYRLVPLDEQFNLSVEGYLQANGGILIANPNAPTGM
                     GVPLESIEKILRYNENNVVVLDEAYVDFGGESSVGLIARFPNLLVVRTLSKSYSLAGL
                     RVGFALGSKELIDGIIRVKDSVNSYTVDRLAQAGAREAIRDDAYFQETKSKIIRTRDR
                     VSSRLGNFGFRVLPSQANFLFVSHPQTPARVLFQKLREQGILVRFFDRPGIDNFLRVS
                     IGTDGEMDLFLEAVARICEKPPK"
     gene            14992..15369
                     /locus_tag="PLA58_05525"
     CDS             14992..15369
                     /locus_tag="PLA58_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOR62289.1"
                     /translation="MSLTIRMETKRPGFWFVSLIGRLDQTTAEECDAKVSSLLNPETR
                     AVVFDMTGLNYISSMGLRIILKTRRFMSGQGGAVYMMNVQPQIEKVFEIANLLQGMRL
                     FADTKEADEYFDAIQKSVLESST"
     gene            complement(15434..17677)
                     /locus_tag="PLA58_05530"
     CDS             complement(15434..17677)
                     /locus_tag="PLA58_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOR62290.1"
                     /translation="MVKHLNAKKLQTLLDVPRKVAALETLNDILDELMKITTTELGAD
                     RGTIFLNDAETGELYSRVAQGNFHREIRLLNTSGIAGAVFQTGKSLIIPDAYSDERFN
                     KSVDEKTGYVTKSILCVPIKAAKNEIIGVTQVLNKKEGAFTEEDVMLLEAIATQAAIA
                     LQSTIFTERMNKFRSKEMAFFDVVAEATSEINLGTLLRKVMSEAMRMLDAERSTLFLN
                     DEKTNELYSRLGEGLGTTEIRFPNSVGIAGAVFRTGETINIPHAYADLRFNPSFDKQT
                     GFFTRSILCTPVTNKSGKIIGVTQVLNKRGGPFTKEDEMRLKAFTAQVAIALENAKLF
                     DDVQNMKNYNEGMLESMSNGVITLGEDGKIITCNAAGLRILKVAAPEILNRRVEEFFA
                     GVNAWVLEKIDKVEKTQTQDVIMDADLEFGNNRLSVNLTVLPLLSVEKKKLGTMIVIE
                     DISSEKRMKSTMSRYIDPALTDQIMDCTDDFMGGKSLTATILFSDIRGFTTLTEELGA
                     QGTVALLNEYFTLMVDCIQKQGGILDKFIGDAIMAAFGIPFAHDDDEDRAVRAAISMI
                     TSLREWNQKRAEQGKKPVRIGIGLNTDTIVSGNIGSPKRTDYTVIGDGVNLASRLESA
                     CKQYHASILISENTHRKLKGTYRMRKVDRVVVKGKTEPVNIYEVLDYHTEETYPGLMD
                     AIHHFSHGLDCYQKGQWDRAMEAFRRGLSINGGDQLSQMYIDRCEYMKKNPPSGEWDG
                     VFVMTSK"
     gene            complement(17694..18155)
                     /locus_tag="PLA58_05535"
     CDS             complement(17694..18155)
                     /locus_tag="PLA58_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62291.1"
                     /translation="MGTEPKTNELSLQKENLHLKQSIAALREQMEKLEAAREEAVQKT
                     LHAVQDEITQLRAAVAAMRDQLENTCYEKDCAAQENMAAAGNREKQLTNTIATLRNEL
                     ERREITHQRDMEAITKTHEDEVKQLHRTIQVLRDRLESFNHSSTTKAGTDE"
     gene            18337..18486
                     /locus_tag="PLA58_05540"
     CDS             18337..18486
                     /locus_tag="PLA58_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62292.1"
                     /translation="MQGHIITTGITFGTALAITISYSYNHSVLWAIFHGILSWVYVIY
                     HVLFH"
BASE COUNT         4192 a         4917 c         5064 g         4402 t
ORIGIN      
        1 ttttcgttac ccataaaata tgccaaagaa ttccttgact aagtcgtaaa ttggaggtac
       61 atttattcgt acaataggag gtacatccaa tgccaaatgc accaaaaatt atacccattt
      121 ctgatcttag gcaaaacgca agcgaagtcg tgaagagtgt ttcatcttct cgagaaccta
      181 tttttatcac tcagcgcgga agagctgctg ctgtcatggt tagcatggaa gtttataaaa
      241 acgcacaaca tgaaatagat attttacacc tcctcgccag aggcgagaag gaaatagcgg
      301 caggtaccgg ttatgaactc gctgacgtgt taaaggaagc agatcattat cttgaggctg
      361 caaaaatttg aaaattttat ttacgcccac aggacgccgc cagttccttg gcgccatttc
      421 ttacattcac cgcgacaagc cttctgcggc agtaagcttt cgccaaaaag cagaaaaatt
      481 attaacgcgg ttaaagaagt ttcctgaatc gggaaggcca ctccctgagt ttccagaact
      541 atcttttcgc gaggttattg taagtccata tcgttttttc tataaggtca aggataataa
      601 tgtatggatt gtggcggtat ggcatggcgc tcaattgcct gatgaaccag aaaaaatcgg
      661 gtaaccaggc gctgcacccg atcgcggctc gctgggccgc tccggctcag cttgtcgtta
      721 tcaaaaaata atcttgaaat atatgcatat aatgtgcatg ctatggcatc acattagtat
      781 gaggtaaaaa acatgagaac aacactgaat attgaagata atttaattga caaagcaaca
      841 aaaattaccg gaatcaaaga gaaaacaact ttagttaagc taggattgga agcgcttatt
      901 gccagagaaa gcgcacggag gcttgcaaag ttaggcggaa cgcaaaaaca gctacaggaa
      961 ataccacgaa gaaaaggagc atgatcaatc atggttcttg tggatacttc tgtttgggtc
     1021 tcacatttga gagatgggaa tactgaaatg gcagccttgc tcaataatgg cagggtatta
     1081 tgccacccgt taattgtcgg ggaactagcg tgtggaaatt taaaagacag agccgtcatc
     1141 ctttcttttc ttaaactgtt acccatgtgc attgaagcag aacatgaaga agtattatcc
     1201 tttatcgaaa ataaccggtt aatgggtaaa ggtatcggtt atgtagatgc tcagcttgtc
     1261 gcatcctctg tattaacggg cgttccgatc tggacacttg ataagaaact ggcgcaagtc
     1321 gctgacagtc tgcacgtaaa atataaaaat tgataacaaa atcaatccac ccgattgcag
     1381 cgccaggggc gctgctccgg cttagttttt cgttggcctt aaaatgattt gacattcgcc
     1441 gttgaacggc gtaccgtata tcgtacgtta tggaggtaaa aaaatgacaa catttacagc
     1501 atcagaagca agaaaacgtc tatataatct tgtagatgaa gttaaagatt cacatgttcc
     1561 ggttcagatc gtagggaaaa gaaattccgc catattagta tccgaagaag attggcgagc
     1621 tatggaagaa acactttacc ttgcgtccat tccgggtatg cgagagtcca ttaagaaagg
     1681 tttgaaaact cctgtggaga aatgcaagga ggagcccggc tggtgagttg gaaattggtc
     1741 tttactaaac aagcgcagaa agatgcaaag agaattgcac aatccggcct aaaaccacag
     1801 gcaagccgct tgctggatgt tctcaaaaaa gatccatttc aaaatccacc accctatgaa
     1861 aagctcatgg gtgatctttc aggtgcatac tcaagaagaa taaatataca gcatcgctta
     1921 gtttatcaag tactgaaaga cgttcgcacg gtaaaggtaa tcagaatgtg gacgcattat
     1981 gaatgagtgg cgcagtaaaa aaataaatca gccaacaaat cagtggcccc gatcgctgcg
     2041 ctccggctca cttcggcgtt aacctttcaa ctggaaagca ttagtttgtc ggatataacg
     2101 atattgcaat attgtagatg taagcaagtg gtaaacaacg cgatgagagc tttgaaaaat
     2161 gtcgtttcga ggagcgtgag cgacgagaaa tcttaaggat ggccgtttgt tcaagatttc
     2221 tcccttcggt cgaaatgaca aaaatggccg ttattcgaag ctctcgcgat ttttgtcaaa
     2281 agtaggcatc agccggatga accaacaatc aaggggtaag atattgcaat taatgtcttg
     2341 cccctttata ttttttttca gatgaagtgc gcggcacatc cgtagtagcc ttcagccttc
     2401 agtcttcggt cttcggtctt cagccttctt catctgcttc gtcatctccg aaagtgaaaa
     2461 tttattgaac ggcaaaaaca ccgcgcccag aacgaccaca accatcatcg acaggaaatg
     2521 ataaaccagc gcgaaggaca gcgcttccgg tttggcaatc ccgaacaggc tcaatcccag
     2581 gatgcaggcg taatgccagt tgccgataaa acccggcgcg gtgggaatgg cgatgcccgc
     2641 gatcaggatc accatcacca cgaaagccgc cagcgccggc agatcaaagc cgaatgcaat
     2701 aagcagcgtg taaatggcgg cgacgtccac cagccagacc acggcggaca ggaccagcag
     2761 atacagcagc cgtttgacgt cggtgatgac caggaagccg tcgatgaaat gatggatgag
     2821 gggattgact ttttgcgcaa gcgcgccggg cagacgccgc aggattcggt cgatgatgga
     2881 gacggcggtt tcccggcgcc agaccagccc gatcacgaag gagatcatca tcagggtccc
     2941 aagaaagaaa agaattccgg aggtaaccat ccagcgcggc aggtcgttct gcgccatgag
     3001 aaccgcgatg gtgatggtca gcacggcaag gctgtcgagc acgcgctcca cgacaatcgt
     3061 gccgagcgcc gaagacatct tgacggcgct tttctgggca atgagatagg gccgggcgag
     3121 ttccccgatc cgggccggta tcgcggcaat ggccagaaat ccgacgctgg tcacggcaaa
     3181 gagcgttccc tgggaaattt tttccatcgg ttccaggatg acgccccagc gataggatcg
     3241 cagcgcctgc atgaacacga tgaaaaaaag agacagcgcc gcccagcccg gatggatttt
     3301 cttcagggaa gtcagcgtgt cgttgaagtt aatgccccga agggaaaagt aaatcaggac
     3361 cacgcccagc aggatgccga aaagaaattt cttgttcatg aaaaggccga cgccggtccc
     3421 ggcggggcgt cgctaaaccc taccgttttg ccagttgatc ggcgatcttg tcatggatca
     3481 cctgggaaga cagcttgttg cccatcagac ccacgcgaac gccgacggtt gtctgatttt
     3541 ttgcccgata ggcaatttca attttcacgt tttcgtcgtt gattatggcc gaaatggttc
     3601 ccttggaaat ttcccgaacg ggctgaacct gcgttccgcg catctgtgcg atcgtccttt
     3661 cgcaggcgct ccagacgctg tcgaaagagg ccgcgtagtc cgtcttcagc tcgccgttgg
     3721 cgtacaggta ggttcccgcg cccgcgccga ccaccgctcc gccggcaacc aaagcgccgc
     3781 atcccgcaag agacatcagg gcaaacgcgc aaaccacgag gcgacctatt ttgcttttca
     3841 tcgtgcattc ctccggattg ttgaagttgc gctcatgtaa acatattgcg aaatggattg
     3901 caacttgatt gtcgatggat tttatcgggc tttttcaaat tcccgcagga ggtcttcctg
     3961 ctttcgggtc agctccgttg gaatcttgac aaagacttca atgatctgat ctccgcgccc
     4021 gtatccctgg agatggggga tccccttgcc tttcaggcgg aagatctgtc cggtttgcgt
     4081 tccgcgggga attttgattt tctccgtttc ttttaaggtg ggcacttcaa ttgtcgcgcc
     4141 gagagaagct tgcacgaatg aaatcgggac gcggcacaca atgtcgtctt ctgaacgaac
     4201 aaagaactcg tggtcttcca cctgcagaaa aacatacaga tccccgttgg ggcctccctg
     4261 atctcccgct tctccttctc cgcgcagacg cagccgggaa ccggtttcca cgccggcggg
     4321 gattttcagc tcgacggttt tgcgctgctt ctccctgccc gtgccgcggc aggtctggca
     4381 ggctttggcg atgactttgc cgtggccgtt gcaatgggga caggtggagc tgatcgtgaa
     4441 gaagccgctc gactgaacca cctggcctcg gccgttgcag gtggggcagg tcgaaggcgt
     4501 cgtaccgggc gccgagcccg ttcctccgca ggcctggcag gggtgaagct tttccaggtc
     4561 gatggtggtc gtcagtccga aggcggcgtc cagaaaagag attttcagat cgtagcgcag
     4621 gtcggcgccg gcccttgccg ccgggcgcgt tcgccccctt ccgcgtcccc ggccgaatcc
     4681 gaacacgtcc tcaaaaatgt cgccgaaacc ggagaaaacg tcgtcaaagc cgctgaaccc
     4741 ctgaaagccg gtgttgctca ggccggcgtg tccataggcg tcatagattt ctcttttttg
     4801 ccggtcgctc agcacctcgt aggcttcggc cgcttccttg aacctttcct ccgcctgctt
     4861 gtttcccggg tttttgtcgg gatggaactg catggcgatt ttccggtaac attttttcag
     4921 ctcttcttcc gaggcggttt tgccgacgcc cagaatttca tagtaatccc tcttggtcat
     4981 gcgctttgtt ttgcttcctc cgctttgatc atggtagcga gcgcgttggc ccgtccggtg
     5041 tccacgattt tcttcagagc ctgcccggca aaggaaaatt ttttaagacg aacggctgtc
     5101 tgcgcgatat tctcccagtc ggcgtcctga agttccagcc cctgcgcctt cgccgcgccc
     5161 tgaaacagaa aaacatcgcc gatttccagc gcttgttttc gaattttttc tatgccggcc
     5221 gtgtgggatg cccgggcata aaagtccagc gcatcggaaa aggctcccgc ttcccaatac
     5281 atatccccgt agctgatcag cgattcggga gaggttttat cgatgtacag tattttttgt
     5341 tttaatcggt aatccggcag ctttcttgcg atcaaggaat gcctcctcgt aaaatgacaa
     5401 cgcaaagata aacaccgcgt aatgtaataa cggtcctgcg tctgtcaatg gctgggtatt
     5461 attttaccag acgggaaaac gcttcttcat actttgaccg ggttttctcg atgatatccg
     5521 gcggcaactc gggcgcgggc ggcttctggt tccaggcaat ggaaagcaga taatctctca
     5581 gaaactgctt gtcaaagctt ttttgcgggc gcccttcttc gtaatcgtcg gccggccaga
     5641 agcgcgacga gtcgggcgtc agtaattcat cgatcaaaat caattgatcg ccgatcaggc
     5701 cgaattccat tttggtatcc gcgataatga tcccggcctt cagggcgatc gccgccgccc
     5761 gttcgtaaat ggccagggat ttttgaatga cctgttcggt aatttccggg ctgatcaggt
     5821 cctccatttc cgagcgggtg atgggagcgt catgttcgcc ttccggcgct ttggtcgtcg
     5881 gtgtgaaaac cggctgcggc agcctgcagg attccttcaa atccccgggc agttggatgc
     5941 ccgaaacgga ttggtcgcgg cggtattcct gccagccgga cccggccaga tagccccgta
     6001 cgatgcattc caccggcaga ggcgttgctt ttcgaaccag catgcttcgc ccctgcagga
     6061 cttcggcgta gggctcgcac tccgccggaa aatcggcggc gtccgcggac acgatatgat
     6121 tgccgataat gtcttccatc ttctgaaacc agaaaaggga catcttgttg aggatggcgc
     6181 ctttgcccgg aatcggattg ggcatgatga catcaaaagc cgatatccgg tccgtcgcca
     6241 ccagcagcag atagtctttc acggtataaa ggtcgcgcac cttgccgcgt gtgagaaatt
     6301 tcagctttgg aaaatgagtt tgagcaatcg ttttatacat gggtcgtttt ttccctctat
     6361 cgaacggatg ggttgtgcaa tttttcgtgg ccgatggtgg acgttggggt gcggccgtag
     6421 ttgtggccgg gcagcacctt ggtgtcgtcc ggcagcgaaa aaagttttgt tttgatggcc
     6481 cgatacaggg tttgccagga gccgccgggc aggtcggttc ggccgacggc ctccacaaac
     6541 agcgtgtcgc ccgtgaaaac atagcccggc gtgtaaaggc acatgccgcc gggcgaatga
     6601 cccggtgtat ggaggacttt cagttcgata ttgccggcgg aaataatctg cccgtcggaa
     6661 acgaggaggt cggcaggcgg cgattgcgcc gcgccgaaca ttcgaagcat ggcgggcggc
     6721 gtcgaagtga gcatgaaggc atcgtcttca tggatgatga ttctggcgcc tgtgagtttt
     6781 ttcatctgag cattgccgcc gatatggtcc acgtggccgt gcgtgttgac gatggaattg
     6841 atgcgcagtt tgttttttgc cgccagggcg agaatttcat gaacgttgtc ggctggatca
     6901 atgaccagcg cgtctccggt gacggggtcc cccaccagat aggcgaaaac cgccatgtgg
     6961 cccacctgca tttgctgaat aaacatgatt tgatctcctc aattttacgg ggaggtaaac
     7021 tagcatgatc gcatggaatt cgtcaaatta aatccggcgc cggaaaacag cgccgaaaaa
     7081 accgtccatc gaatggcgat gagggtaact cctgaataat ccacgactat caatgagccc
     7141 ttgcacaacg ggggaatccg gcagcaaaag ctgaaattgc gggaaatccg ccagaaaccg
     7201 ccgcaccacc ctgtcatttt cctgagaaaa aagagagcag gtgcaataca ccagaaaacc
     7261 gtttttggaa accgccgccc ccgcgttttg caggatggat tgctggagat cggcatacag
     7321 atggatatct tttggtttta gccgccattt gatttcggga ttgcgccgca gcgtgccggt
     7381 tcccgagcag ggagcatcca cgagaaccgt atcaaatgca ttttgatatt gggccgacag
     7441 agggcgctcc agatccacgc gggctgtttt gacgatggtt atgcccattc ttgcggcgtc
     7501 attttgcaat tgcacaagtt tttccgcatc ccggtccagt gcgacaatca cgccttcgtt
     7561 tttcatcagg gcggccagat gcgtggtctt tccgccgctt cccgcgcagg catcgagaat
     7621 cttttgtcct tttcgggggt tgaccagata ggcaaccagt tgggacgcct catcctgaag
     7681 gcgcaaaagc ccatccgcaa agaaaagagt tttctgaatt ggcaaagaag aagaggacag
     7741 acggatgccg tcgggagaca gccgggtttc ttcagcctga aaattttcgc cggccagttt
     7801 ttcaatgagc tcccggcgac cggatttcag tgtgttggcg cgggccgtca ggggcggcag
     7861 ttcgttattg gcggcgcaca acgcctcggt ttccatccgt ccgaaaacat tcagccattc
     7921 ttcgaccagc cacaaggggt gggaatgaaa agcggcgata tattccgccg gatttttgtt
     7981 ctcgtcgggg aaagaccagg ggccggggct gcgcaaataa cttcgcagga cggcgttgac
     8041 cagcccgcgc gccgccggct ggacgcgccg ggtaattttg accgcttcat ccacgaccgc
     8101 aaaggccggc agccggtcgc tgaattggag ctgatacaat cccgtgcgca ggacattttt
     8161 gatattctct tccattttca agtagttgcc gcgatacaga cgggccagaa tccagtccaa
     8221 atgcccctgc atcctcaaaa cgccatagac cagatgtgtg agcaggcggc cgtccgccgt
     8281 ccgggaaagg ttttgttttt caagacaaga atccagcaga tcgccggcga aagcgtcgct
     8341 ttgtgaaacg cgggtcagga tttccaccgc ctgatgtcgc accgtttttt gcataaggtt
     8401 tcgtatcata aaatccggtg taatgaacaa tatatttctt gcttttcgat gctgatctgt
     8461 taagaaaaac aacaacactc gttgtaaaaa tttttaagga ggaaagtatg tcgttaaatc
     8521 agttaatcga ttcgcgtgat gtccgtttta ccctgtttga gatgctggaa ttggagaaat
     8581 tgaaccggtt cgacgccttc aaggattttg accgaagcgt ttacgaggat acgctcgaac
     8641 tggccgagaa aattgccgtt cagcagtttt atccgaccaa tgccgaaggg gacaagacgg
     8701 gcctgaagtt cgatcccaag acgggcgagg tcaaggttcc cgaatcgttc aaaaagggct
     8761 tcaatgccta tatcgagtcc ggattccatt ccctggctct gccgcaggaa atagggggaa
     8821 cgggtctccc ctccagtatt tccatggcct gcgcggaata tttcaatgcc ggaaacacat
     8881 ccctgacgat gtattgcggc tccatcaccg gggcgattgc cattattgcc ccctttgcga
     8941 ataaggaatt gtacaacatg gtcgtcccca agctgatgga agggaaatgg ggcggcacca
     9001 tgtgtctgac ggagccgggc gccggatcgg atgtcggggc gctgaaaaca aaagcggtca
     9061 aacagcccga cggcacctat ctgatcaccg gcacaaagat cttcatctcc aacggcgaac
     9121 acgatatgac cgagaacatc attcacccgg tgctggcgcg catcgaaggc gatccggaag
     9181 ggaccaaggg gatctcgatc ttcgtcgttc ccaaattcct gatcaacccc gacggctcgc
     9241 tgggcgaacg caacgatatg atctgcgccg gcatcgagca caaaatgggg ctcaagggga
     9301 atgcgacctc catgctgacc ttcggcgaca acggcaagtg cgtcggctac ctcctgggca
     9361 aagaacgcga aggcatgaag atcatgttcc atctcatgaa tgccgcccgc atccacacgg
     9421 gcgtccaggc cctgggttgc tccagcgccg cctacctgca cgccgtgacg tacgcccgca
     9481 accgggtgca gagcgcgctc atcacgaatc ccaaaggcgg ccccgtgacc atcatcaatc
     9541 accccgacgt gaagcggatg ctcctgtaca tgaagagcaa cgtggagggg atgagcatgc
     9601 tgtgcctctt cctggcgctg catgaggaca tcatgcgcgc gtcgaaagat cccgaagaag
     9661 tgaaaagggc gaccggcatc gtcgaaatcc tgaccccgat cgtcaaggcc ggcatctccg
     9721 acgcctcctg gctcgttacc gccgaggcga tgcaggttta cggcggctac ggcttctgcc
     9781 aggaataccc ggtcgagcag tacgcccgcg acaccaaggt attctcgatc tatgaaggga
     9841 ccaatgccat ccagtcgctc gatctggtca tgcgcaagat cctcatgaat cccgagatgt
     9901 acaattacgc tcagcttaag caatggatga gcgacacgat tgccaaagcc aaaggcattg
     9961 ttgacgacag gtatgtcaac ccggtcgtcg agggtctggc aaaacttgaa gaagtgatcg
    10021 agatgatgaa gaagcagttg ggcgagatga aaattcttct cctgctcaac aacgcaaccc
    10081 ccttccagca ggccatgtat ccgctgattc tggcctggct gcatctgtgg agcctcaccc
    10141 tcaccacgcc caaagccaaa gcgctcgtgg gcgacgccaa gggcgcggat cgcgcgaaga
    10201 tcctcgcgga aaatccggaa gccgcctact acagcggtcg cgtgctctcg tcgcagttct
    10261 atatcggcgc ggaatttcca aaattcttcg gccgtatcgc agccatcgtg aacaatgaag
    10321 gcgcggcgct gaaggtcgag gcggataact tcaccggagc gctgaaagaa tagcgaagcg
    10381 acggtcgaaa gagctggatc ctgcagcggg gaggggcatc catcggctgg cccacgcgct
    10441 ggcaaaagcc tcgatctggg ggaaggatgg cggacgacgg aaaccgtctt ccgccttatc
    10501 ttaattattc atttcggaaa aggagacgca acatgaaagc tgaagatgta aaaaagattg
    10561 ccataatcgg cgcgggggat atggggcacg ggatcgcggc ttcctgcctg ttgggcggat
    10621 atgccgtcgt gctgcgcgat atcgaacaga aatttgtgga caagggcgtc gcggggatta
    10681 tcaagagcct ggagaaattc agggagaagg gcaagattac gccggatgcc tttgcccacg
    10741 cgctggtgcg ccttaccccg atagtggacc ttcaggaagc cgtccgagac gcggatttcg
    10801 tgattgaagc cgtgccggaa aagctggagc tgaagaaaag cgtttttgcc gacatcgaca
    10861 aatatgcgcc gaaacacgcc attctggcct ccaacacctc caacatcagc atcaccgaga
    10921 ttgcctccgc cacgcagcgg ccggacaagg tcatcggcta tcattttttc aatccggcca
    10981 ttttgatgaa gctggtggaa gtgatcaagg gcaggggcac gtccgatgaa tccgtacagg
    11041 tgggctatga catcgccaaa aagatcaaaa aggttcccgt catcgtcaag aaggattccc
    11101 cgggcttcat ctacaaccgg gtcaacgaac cgacgctggt tctgctgtcc aaaatcctgg
    11161 aagcgggcca tccgtccccg gaggaatttg acgcggcgtt caagcccctg atggcgatgg
    11221 cgccgttcga actggtggat tatgtcggca tcgacgtggc cgcgcacggg ctggaatatt
    11281 tcgcgcaggt tttgtccccg gactacaagc cctccgaggc gatgatgtcc tacctgaaga
    11341 tcaatcatct gggcaaaaaa accggcaggg gattttatga ctggtcggcg ggcaggcccg
    11401 cgatcgatct ggcaaaggcg accaaggaat acgacatcaa tcagttgatc gcgctgcagg
    11461 tcaacgaagc cacgaagctt ctggaagagg gcgttgtgga cgatcccaag gagatcgatc
    11521 tggcgatggc caacggcggc ggctcgccct tcggcccctt tgcgctggcc cagggcattg
    11581 gttacgcgga gctggtgttc aagctggggg aaatttacaa aaagttccct ctggacatct
    11641 tcaagcccac gaaaaccctg aaagagggca agatcaaact gtgatgcagg cgtaaaaagc
    11701 ccatgtgcgg taggtttgtt ttgatttcgg atttgtcggc tgtcgcgcaa agctttggcg
    11761 tgaccgatgt ctcccttcat tttttacccg accgcaatat ccatccgggc cggcatattc
    11821 ccgcggtgat ccgaaccggt caagggaata tgccggnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnncatc cgggccggca tattcccgcg gtgatccgaa ccggtcaagg
    12001 gaatatgccg gtctcttttt tgtgggggct ggtccccttc tgggcgaaag atccgtccat
    12061 cggaccgagg ctcatcaatg cccgcgctga gacgattgcg caaaagcccg cctttcaaaa
    12121 tgctttccgg aagcgaagat gtctgattcc cgccgacggg ttttacgaat ggaaaaaaga
    12181 cggggcctcg aaaaagccct tccggtttgg cctgaaatcc ggcgggccgt ttgtttttgc
    12241 cggcctctat gagcaatggg cggccgccgg tcaaaaaccg ctggacacct gtgccattat
    12301 cacgacgagc gccaacgagg ttgtggcgcg tgttcacgac cgcatgccgg tcatcgtgcc
    12361 gaaatcttca caggagctgt ggctgaatcc ggaacttcag gatcccgccc ttcttgccgg
    12421 tgtcctgaaa ccctatccgg cggaagaaat gatctgtgag ccggcagaca tttgaccgga
    12481 tgggtccttt ccgcagccgc cgcgcttttc accggggtat tgttttccgg caggattgcg
    12541 gcccggatga cccggagaat tggtttgcca aaaagagcaa tcatctgcta aagagcaggc
    12601 cgacttcgca gaaaggattg tcaacggaca aacccatgac gcttaaaggc atcatcaccg
    12661 ttatccgcgt cccttttttg attctggcgc tcacgctggg cattctgggc gccgccgtcg
    12721 cctggtatga atccaggcgc ttcggctcgc cgtttcatct cgggtatgcg gcgctggcca
    12781 cgttcggtct tctggtcgcc catgcggggg ttaatatatt gaacgactat ttcgacgcca
    12841 aaagcggtct ggactataaa acgctgcgca ctcctttcag cggcggaagc ggcgcggcgc
    12901 cgacaggcct gctgacggtt gcccaggttt tgtggctggg gattgcctgc tttgttatcc
    12961 tgattccgat tgcctggttt tttgtcgtga aagcgggctg gatgctcctg ccgctgcttg
    13021 tggccgccgc cgccctgatc gttttttaca cgcccctcat cctcaaaatg ggatacaccg
    13081 agtggtcgcc gggcctggga ttgggcattc tgcccgtcat gggcgcctac ttcgttcata
    13141 ccggtgcgta ttcaatgagc gcctttattg cctcgatgcc ttcttttttt ctggtgcaca
    13201 acctgcttct gatcaacgag tttccggacg tggaggcgga tatcaccgtg ggcagacgga
    13261 cgttgccgat tgtcgccgga aagaaaaatg cggcttattt cttttctctt ttcaccgtgc
    13321 tggtctattg ctgggtcatc ggagcggttc tttgcggttc catgccagcc ttcacgcttt
    13381 tgtccctgct gaccgttccc attgcggtcc gggtcatcca aggggcgctc cggtatgacg
    13441 acagggaaac gctgctcaaa gccatgtccg ccaacgttct gctggttctg ctcacccagg
    13501 cgctgattgc ggcgggatac attgtaagcg gcatcctgct cataacatga gagctttgaa
    13561 taacggccgt ttttgtcatt tcgaccgaag ggagaaatct tgaacaaacg gccatcctga
    13621 agatttctcg tcgctcacgc tcctcgaaat gacatttttc aaagctctca acatgatgct
    13681 ccttttatgt gaaaatgccc cctccctgta ttcctccccc tcgatggggg agggaagcga
    13741 gggggtgatg atcattctgt gttatgcccg ccacggccat ggagggtaga agaaaagata
    13801 agcatggaaa ttttggcgca tttcctatat taaatttaca aattgggaaa aagactacac
    13861 tacggataca aagccatgag ccggttctgg agccagacaa ccaaaaacat caagccttat
    13921 gttccgggag aacagcccaa agaccggaaa tacgttaaac tcaacaccaa tgagaacccg
    13981 tatccgcctt ctccaagggt ccttgaggtt atccgaagcg cggccgatga aacgcttcgg
    14041 ctttatccgg atccgtcggg cgatgcgctc cgggacgcca tcgcctcgtc cttcggtttg
    14101 attagggaaa atgtttacat cggcaacggc tcggatgaac tcctggcttt ctgctttccg
    14161 gcgttttttg agccggaggg gcttccaatc ctttttgccg acgtcaccta cagcttctat
    14221 ccggtgtacg cgcggctttt ccggattccc taccgtctgg tgccgctcga tgagcagttc
    14281 aatctgtcgg ttgaaggcta tcttcaggca aacgggggaa ttctcattgc caatcccaac
    14341 gcgcccaccg gcatgggggt gccgctcgaa tccatagaaa aaatcctccg ctacaatgaa
    14401 aacaacgttg tggtgctgga cgaagcctac gtcgattttg gaggcgaatc atctgtcggc
    14461 ctcattgccc gttttccgaa tctgctggtg gtgcgcacac tgtccaaatc ctattcgctg
    14521 gccggattgc gggtgggatt tgcgctgggc agcaaagaac tgattgatgg aattatccgg
    14581 gttaaagatt cagtcaattc ctacaccgtt gaccgtctgg cccaggcagg cgcccgggaa
    14641 gccatccggg acgacgcgta ttttcaggaa accaaatcaa agatcatccg cacccgtgac
    14701 cgggtatcgt cccggctggg aaatttcggc ttccgggttc ttccgtcgca ggccaacttt
    14761 ctgtttgtca gccacccgca aacccccgca cgggttctct tccaaaagtt gagggagcag
    14821 gggattctcg tgcgcttttt cgacaggccc ggaatcgata atttcctccg ggtgagcatc
    14881 ggaacggacg gggaaatgga cctctttctc gaggccgtcg cccggatctg cgaaaagccc
    14941 ccgaaatgat ttcgccgggc gccggttcga tttcaagaaa ggagaaaagc aatgtctctg
    15001 acgatccgca tggaaaccaa aaggcccggg ttctggtttg tttcgctcat cggacggctt
    15061 gaccagacga ccgccgaaga atgcgacgca aaagtctcct cgcttctgaa ccccgagacc
    15121 agggccgtcg ttttcgacat gaccggcctg aattacattt ccagcatggg tctgcgcatc
    15181 attttgaaaa cgaggcgatt tatgtccggc cagggcggcg ccgtttacat gatgaatgtg
    15241 cagccgcaaa tcgagaaggt gtttgagatt gccaacctgc tgcagggaat gaggctgttt
    15301 gccgacacaa aggaagccga cgagtatttt gacgccatcc agaaaagcgt tttggaatcc
    15361 tcaacgtgaa gccacaaacg cctcttttcg cgagcttgcc ccgcatcgcg ccggggatat
    15421 ttttccggtg cgcttacttt gacgtcatga caaagacgcc gtcccattcg ccggacggcg
    15481 gatttttttt catatattcg caccggtcga tatacatttg agaaagctga tcgccgccgt
    15541 ttatggacaa tccgcgccgg aacgcctcca tcgccctgtc ccattgccct ttctgatagc
    15601 agtccagacc gtgcgaaaaa tgatggatgg cgtccatgag gccgggatac gtttcttcgg
    15661 tgtggtaatc gagcacttcg tagatgttca cgggctctgt ctttcccttg accaccaccc
    15721 ggtccacctt ccgcatgcgg tacgtgccct tcagtttgcg gtgcgtgttt tcgctgatca
    15781 ggatggacgc gtgatactgt ttgcaggcgg attccagacg cgaggccaga ttcacgccgt
    15841 cgccgatgac cgtataatcc gtgcgtttcg gtgaaccgat gtttccggag acgatggtgt
    15901 ccgtgttcag gccgatcccg atccggaccg gctttttgcc ctgttcggcg cgcttttggt
    15961 tccattcccg gaggctggtg atcatagaga tggctgcccg cacggcgcgg tcttcgtcat
    16021 cgtcatgggc gaaaggaatg ccgaaagccg ccatgatggc gtctccgatg aacttgtcca
    16081 ggatgccgcc ctgtttctga atgcagtcca ccatcagcgt aaagtactcg ttgagcaggg
    16141 cgacggtgcc ctgggctccc agctcctccg tcagcgtagt aaagccgcgg atgtcggaaa
    16201 acaggatcgt ggccgtcagg cttttgcctc ccatgaaatc atccgtgcag tccatgatct
    16261 gatcggtgag cgccggatcg atataccggg acatggtgga cttcatgcgc ttctcgctcg
    16321 agatgtcctc gatgacgatc atcgtgccca gcttcttttt ctccacgctg aggaggggaa
    16381 gaaccgtcag attgacggac agacggttgt ttccgaattc caggtctgcg tccataatga
    16441 cgtcctgcgt ctgcgttttc tccaccttgt cgatcttttc cagcacccag gcattgacgc
    16501 cggcaaagaa ttcctccacc ctgcgattca gaatctccgg tgcggcgact ttcagaattc
    16561 gaaggcccgc cgcgttgcag gtgatgattt tcccgtcttc gccaagcgtg atcacgccgt
    16621 tggacatgct ttccagcatg ccctcgttgt agttcttcat gttctggaca tcatcgaaga
    16681 gtttggcatt ttccagggcg atggcgacct gcgcggtgaa ggccttgagg cgcatttcat
    16741 cttccttggt gaacggtcca ccccgcttgt tcaggacctg cgtgacgccg atgattttgc
    16801 cggatttgtt ggtcaccggc gtacagagaa tagagcgggt aaaaaagccg gtctgtttgt
    16861 caaacgaggg attgaagcgc aaatcggcgt aggcgtgggg gatattgatg gtttcccccg
    16921 tccggaatac cgcccccgcg atgccgacgc tgttggggaa gcggatttcg gttgtcccga
    16981 gtccctcgcc cagcctggag tacagctcgt tggttttttc atcattcaga aaaagggtgg
    17041 agcgttcggc gtccagcatc cgcatggctt ccgacataac cttgcgcagg agtgtgccca
    17101 ggttgatttc ggaggtcgct tcggccacca cgtcaaaaaa cgccatttcc ttcgagcgga
    17161 atttgttcat ccgctccgta aagatggtgc tttgcagcgc gatggcggcc tgcgtggcga
    17221 tcgcttcgag aagcattaca tcttcttccg tgaaagcccc ttcctttttg ttcaggacct
    17281 gggtgacgcc gatgatttcg tttttggctg ccttgattgg aacgcagaga attgatttgg
    17341 tgacatagcc tgttttttca tccacggatt tgttgaagcg ctcgtcgctg tatgcgtccg
    17401 gaatgatcag gctctttcct gtctggaaaa ccgcgcccgc gatgccgctg gtgttgagca
    17461 gccggatttc ccggtggaaa tttccctgcg ccacccggga gtataattcg ccggtttccg
    17521 catcgttgag aaaaatcgtt ccccggtccg cgcccagttc ggtcgtggtg attttcatca
    17581 attcgtccag gatgtcgttc aaggtttcca gcgccgccac ttttcgcgga acgtccagca
    17641 gcgtttgcag cttctttgcg ttcaggtgct ttaccattga tttattccgg gagttattca
    17701 tcggttccgg cctttgtggt tgacgaatga ttaaaggact ccagacggtc cctcaaaacc
    17761 tggatggtcc ggtgaagctg cttcacttca tcctcatgcg ttttggtgat cgcttccatg
    17821 tcccgttggt gcgtgatttc ccggcgctcc agttcgttgc gcagggtcgc gatcgtgttc
    17881 gttaactgct tttccctgtt gcctgcagcg gccatgttct cctgggccgc gcaatccttt
    17941 tcatagcagg tgttttccaa ctgatcccgc atggcggcaa cggcggcgcg aagctgggtg
    18001 atctcatcct ggaccgcgtg gagggttttc tgcacagcct cctcccgggc tgcttccaat
    18061 ttttccatct gctcgcgcag cgcggcgatg ctttgtttca ggtgcagatt ttctttctgc
    18121 agcgatagtt cgttcgtttt cggttccgtt cccatagacc tcaatgcggc gcgttgcgta
    18181 tttagcgttg gcgcgcatta tagggccaat ttctgcgtaa tttcaactga aataaatcag
    18241 gggaacggcc acccggtgtt ttcttgacag cgaagagggg agtctctata atgccgccat
    18301 caaaagcagg attttccatt attgcaggag gttgccatgc agggtcatat cattaccacc
    18361 gggattacat tcggcacggc gctggccatc accatcagtt acagctacaa tcattcggtg
    18421 ctgtgggcaa ttttccatgg gattctctcg tgggtttacg tgatttacca cgttcttttc
    18481 cattgatgcg gccgggcgcc aaccggcatg accagccccg ccgaacagaa gataaggatc
    18541 gaggcctttt gtcttttacc ccggatgctg gatttcgatt tcgcagccgg gatactcact
    18601 tcctcattaa aaatgcggtg aagatattct tgacatccat gaccgcgttt tctatgcttc
    18661 ccccgcgaaa aagcc
//
LOCUS       DAOSRM010000040        17702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3327_length_17702_cov_121.425908, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000040 DAOSRM010000000
VERSION     DAOSRM010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 17702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17702
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_17702_cov_121.425908"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="recA"
                     /locus_tag="PLA58_05545"
     CDS             <1..1018
                     /gene="recA"
                     /locus_tag="PLA58_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOR62293.1"
                     /translation="MCADMDRSKAVDLAISQIERQFGKGSIMKLGSHESVADVPAIST
                     GSLSLDIALGTFGVPRGRVVEIYGPESGGKTTLALHIVAEAQKKGGIGAYIDAEHALD
                     VVYAKNIGVNTDELLISQPDTGEQALEIAETLVRSGALDVLVVDSVAALVPKAELEGE
                     MGDAQMGLQARLMSQALRKLTGSISKSKTTVIFINQLRMKIGVFFGNPETTTGGNALK
                     FYSSQRLDIRKMTSIKNGQDVVGFRTRVKVVKNKMAPPFREAEFDIIFGEGISREGDV
                     LDLAAANGIIEKSGAWYSYRGDRIGQGRDNSRIFLKENPDVLAAVEKDVREKLGITAK
                     DQEE"
     gene            1020..1505
                     /locus_tag="PLA58_05550"
     CDS             1020..1505
                     /locus_tag="PLA58_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOR62294.1"
                     /translation="MKALDLEAAKQKAYRLLSLRPHSARELEKKLLEKGFPKTIVKEA
                     LEKLHELKYLNDASFAGSRARQLAAGKLWGNRKITACLREKGVDDRLIDDAIASARRE
                     LSEEEAIALWLKKKASKGRPDRKEKQKIFQSLMGRGFPPGLILKMLGTPAEEEADDEN
                     G"
     gene            1489..4131
                     /gene="alaS"
                     /locus_tag="PLA58_05555"
     CDS             1489..4131
                     /gene="alaS"
                     /locus_tag="PLA58_05555"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOR62295.1"
                     /translation="MMKTGDAIRESFLKFFESKGHTRVASSPLIPKDDPTLLFTNAGM
                     VQFKNAFLGLENRGYTRAATCQKCARAGGKHNDLENVGVTARHHTFFEMLGNFSFGDY
                     FKEEAIAWAWEYLTDVVRLPKENLWVTIYENDDEAFKIWHEKMKVPADRIVRMGEKSN
                     FWMMGETGPCGPCSEIIFDQGEGTGCGRPECDIFCECDRHLEIWNNVFTQFDRDASGK
                     LTPLPRPNIDTGMGLERLTAVIQGVKSNYDTDLFAPILRFVEKTSGKIYGKNADNDIS
                     IRVIADHSRALTFLIGDGILPANDGRGYVLRRILRRAARHGKMLGIGKPFLNEAAMVV
                     IDMMKYTYPDLADKAPYITKVILNEEQRFMETLDAGLRILQEETETLKKAGKTILPGK
                     MIFKLYDTFGFPTDLTADIVKKDGITLDTEGFEAEMERQRERARGAWKGSGEEAVAEC
                     YLKASSSGVASVFCGYETATQDEAKITAIFVDGKSVETAPAGEKAEIVLDTTPFYGES
                     GGQTGDTGYLEGDGFRFHVEDTKKPVENFIVHQGVILSGEIKVGAAARLIIDLERRRA
                     IAANHSGTHLLQAALKAVLGDHIKQSGSLVNAERLRFDFTHFSRISEEELRRVETIVN
                     DMIRRNVSVQTDVCALEEALKTGATAVFDEKYGATVRIVKMGEMSMELCGGTHVERTG
                     DIGFLKILHESAIAAGVRRIEAVTGKEAVLHVQKIEEELKRTAGLFKAGHLEVFDRAE
                     KLLKQSRDQEKEIEALKGKLAAKDSGDLMGRVKEIAGVKVLAAEVSIADAKTLRDFGD
                     KLRDRLASGVILLGSKAEGKALLLCMVTKDLTGKFSAGNIVKELAPLVGGKGGGRPDM
                     AQAGGTKPENLAAVFPALEKLLSA"
     gene            4151..5425
                     /locus_tag="PLA58_05560"
     CDS             4151..5425
                     /locus_tag="PLA58_05560"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOR62296.1"
                     /translation="MNISILTLAVVFLLIAIRQIGSARLQIWQIMLGGAVFVILTGEI
                     SPRAAFHAIHFDVIFFLFGMFIIGQAMEDSGYLTHICHILFGRAKSVDALVLAILFTM
                     GIGSAVLMNDTLAIIGTPIMLLLARRNHISPKLLLLCLAFAITTGSVMSPIGNPQNLL
                     IALGGNIGNPFLTFFQYLFAPTIVNLFMAYIVLKVLYKKEFHRTELQPGIEDIRHVRL
                     AKIARLSLVLVVCLILAKVALAVMNLFTPVPFDFKLTAIALIGCSPILIYRFMSKKKT
                     YRFHILRRIDWFTLVFFASMFVLMESVWRSGFFQHLLSAFSLNMTGVLVMMILSVLVS
                     QLISNVPMVALFLPLMLSAGAGAKELMALAAGSTIAGNLFILGAASNIIIIQHAEKEN
                     RGESLSFLEFAKAGIPLTIINTAVYLLFFEIF"
     gene            5562..7541
                     /locus_tag="PLA58_05565"
     CDS             5562..7541
                     /locus_tag="PLA58_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HOR62297.1"
                     /translation="MKRIAKRRGLAALCLAAIFLLTGCGKDNGVGGVAGDAGKAVIAA
                     NQAMAQKLDFSQDRDFQDAQRGLIAKPHGQIRAADGTVLVDFAAFHFVEGDPPPTVNP
                     SLWRHAKLNARIGLFKVTDGIYQLRGFDLGNMTLIEGKTGWIVVDPLTCRESAAFSFA
                     FAQKHLGEKPVSAMIITHSHVDHFGGALGVVSPREAALRKLPVIAPAGFLEESISENL
                     MVGTAMGRRSSYQFGKDLERSAKGMVDTGLGKTTAYGSIGIIRPTVIIDQPVREMLVD
                     GLRFVFYNTPGAEAPAEMTFVLPEKKAFCGAELLSQTMHNLLPVRGAKVRNALLWAQY
                     LDDALEYTAGCEVYFGQHSWPIWGSNRIKEFITLHRDVYRYTHDQTVRLINAGYTPRE
                     IADAVQLPKSLESHWGARGYYGDLRHNVKAVYQFYMGFYDGNPANLNPLPPQESAKRY
                     LELIGGADKAADAAEAAFRKGEYRWAAELLNMVVFGKPDHTKARKLLSRTYDQMAYMS
                     ESAIWRNSYLTAAQELRDGPPRKSIDRSGALDMMENIPAERFLEVMACSLNGPAAEGK
                     NYKFNLVFGDTGETFVLWIEHAVLHHRKGKAQDAQATLTLTRPIWIKMMAGTAGIKDT
                     LLSDDLKISGSRIDLIRFFALLEKPLGVFPIVTPR"
     gene            7589..8077
                     /locus_tag="PLA58_05570"
     CDS             7589..8077
                     /locus_tag="PLA58_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR62298.1"
                     /translation="MEHRIRTCDQEDIEVLVEIIREAFQEVADRFGLTEQNSPTHPSN
                     CRTEWLMREMNRGVVFYVLEAQGRPVGCVALEKINDEVCYLERLAVLPREQKKGFGEA
                     LVKHALSQARLLKAFRVQIGVIADDSALQNWYEKLGFEEVERKNFPHLMFEVAFMAYY
                     FL"
     gene            complement(8211..9701)
                     /locus_tag="PLA58_05575"
     CDS             complement(8211..9701)
                     /locus_tag="PLA58_05575"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOR62299.1"
                     /translation="MRSKQDYITGLSKMKRNLYYDGQLIDRTDELQMRCLNTIGTTYD
                     EAERPENRDLMTAVSHLTGERINRFTHIHQNTEDLHRKQDMTRMLCQKVGGCIQRCMG
                     IDAANAVYNVSFEADKVKPGETRYHENFKKWLLRFQQEDLVGCCAQTDVKGDRLLRPA
                     DQPNPGSYVYIKERLKDGIVVEGCKLHISEASVADEVLVVPTRALREQDKNYAVAFAI
                     PGDWDGLKQVLTIHSLREREHYQRGFMEGSTDSYMIFENCFVPWERVFLAGEWQHGGI
                     NALLFALFHRHSYSGCKPAIGDVILGMAALAAECNNIQKESHVREKLAEIILVTELGY
                     AAGYTASDLGGPKVFMPGKGFVPYGPGSYIPHSIYCNVGRCLSGEAVWRESEILCDIS
                     GGVTATFPHEKDFLNPETGEALLKYTKRSPKISAEDQAQFWRYLGDKLCSATGGVRNI
                     GAYHGGGSPIMEQIAITTQYDIESRKKLVKYIAGMSGGDREALAEK"
     gene            9821..10480
                     /locus_tag="PLA58_05580"
     CDS             9821..10480
                     /locus_tag="PLA58_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOR62300.1"
                     /translation="MDIILYFIDVFIHLDQYLPMIIQSFGVWIYVLVFVVIFCETGLV
                     VTPVLPGDSLLFALGTLAALGALHIEALLILLCIAAVAGDTVNYAIGHYIGPKVFHYE
                     ESRFFKKKYLIKTHEFYERHGGKTIIIARFMPFIRTFAPFVAGIGAMSYARFIVYNIV
                     GGVSWVAIFLLGGYFFGNIPAVKTNFTIVIVAIIIISVMPGFIAYFRQKYLARKAGSE
                     S"
     gene            10537..11400
                     /locus_tag="PLA58_05585"
     CDS             10537..11400
                     /locus_tag="PLA58_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62301.1"
                     /translation="MILKFAVIVAAVLLLLPAHADAKNIVKAGSDILVEEGQTVDNVA
                     VIGGQITVSGLVENNVLAIAGSVVLTSKAVVRGKVIVLGGIVARGSGSTVFGDITEVN
                     AATLSEAFQSAMRGELEGWSLILNVISICFFAIILMIALIMTLLVPRRLTMVTDAIGA
                     NRLKAFFLGILATLMIAPLFMLLAISIIGIFLIPLAFTALLLAVIIGFIASGAILGNW
                     IISRSFRGHRKSLVKETLVGLCLLWLLGWIPLYIGMIIKVFAITIGLGGVLLSLSRKT
                     VRPIENQAPSD"
     gene            complement(11366..12238)
                     /locus_tag="PLA58_05590"
     CDS             complement(11366..12238)
                     /locus_tag="PLA58_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR62302.1"
                     /translation="MAFFTADDGVRLYYEKSGSGKPVVLIHGLTANSLFFRKQIPELS
                     RHFSVIALDLRGHGQSEAGSDHWTLSRLAEDLKQLLAELHISKASLVGWSMGAQVIFE
                     YIRNYSCASIDKIVIIDMTPRLMKADDWSCGLPGVFSRKPGDFGHEDNLFLLSVMLKD
                     WEAYSRVVARRILNKSLYNEKMEFNAEGDFKGKSDLPWLYEQARKNNACVIAAFWIAM
                     ATQDYRAVLKDITVPCLLAYGTESNYYPPENYDYMQQQMPDARVVAFEGCGHALHLQD
                     PDRFNRTALDFLSA"
     gene            complement(12472..13404)
                     /gene="mdh"
                     /locus_tag="PLA58_05595"
     CDS             complement(12472..13404)
                     /gene="mdh"
                     /locus_tag="PLA58_05595"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOR62303.1"
                     /translation="MNQKVTVVGAGNVGATAAQRLAEKALADVVLIDVVEGVPQGKSL
                     DLAQAAPIEKHDAHLAGANTYEASAGSDIVIITAGLPRKPGMSRDDLLATNRGIMKNV
                     VRQAVRYSPDAILIIVSNPLDAMCHVAMEESGFPKNRVIGMAGVLDSARFRAFIAMEL
                     SVSVENVHAMVLGGHGDTMVPLPRFSTVAGIPITELMSPERIEALAMRTRNGGAEIVG
                     LLKTGSAYYAPASAAVEMAESILRDKKKILPCAAYLEGEYGISGLFVGVPVKLGKSGI
                     EEIISLRLLPEEQKALEKSAAAVRELVDAMKRMA"
     gene            complement(13502..14056)
                     /gene="cobU"
                     /locus_tag="PLA58_05600"
     CDS             complement(13502..14056)
                     /gene="cobU"
                     /locus_tag="PLA58_05600"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOR62304.1"
                     /translation="MMIAEIPEKGMTLILGGARSGKSTFAEQTARESGKPVLFIATAC
                     AGDEEMAERIRRHRASRPAGWQTLELPMDIGCSLQTTAAGVIIVDCITLLVNNILLSL
                     PENTSEDIVMEKVRAEMDELIAAQARLGGQWLVVSNEVGLGLVPPYPLGRLYRDALGR
                     ANQALAQKAARVILMVAGIPMVIK"
     gene            complement(14119..14907)
                     /gene="cbiR"
                     /locus_tag="PLA58_05605"
     CDS             complement(14119..14907)
                     /gene="cbiR"
                     /locus_tag="PLA58_05605"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HOR62305.1"
                     /translation="MTYPFRLGTTSYIVPADILTNVRYLAGKVRDVELVLFEVDDGPN
                     NLPANSEIAELVAIARGCDLTYTVHLPLDLRLTDDGFRRHASLDKARRVIERTRALDP
                     WAYVLHLDGKDVLNRSEPASMERWRDQAVLALELVGKWAGGPQRLAVENLEGYPPDFY
                     DPVLDRISVSRTVDVGHLWLDGCDPVAYLKKALPRTRVIHIHGIEDRDHRSAAKAPRE
                     KLKAVLDELVGSDYRGVLTMEVFSEADFLTSRAAIDAAMHANLR"
     gene            complement(14985..>15739)
                     /gene="cobS"
                     /locus_tag="PLA58_05610"
     CDS             complement(14985..>15739)
                     /gene="cobS"
                     /locus_tag="PLA58_05610"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOR62306.1"
                     /translation="KGKRNSMRNLRTAFGLLTTLPVGVPENWRPGDSGRAAFWYPVVG
                     LAVGGLVWLAWFGLNLIFPALVASVLTLAVWVGLTGGLHLDGLADCCDGLLGSASRER
                     RLEIMKDPHLGAFGVIGLFFALMLKAAALGLMPPSSGLSIVLAATLGRWLLLPVSRLP
                     QARPGGMGADFAAGLQTGSIFFAAILPIGLAFLLGMQGWMAVLAALIAAAAVLGLAWK
                     RIGGVTGDVLGALVEVTETAVLLVFTISCRAL"
     assembly_gap    15740..15839
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15840..16867)
                     /gene="cobT"
                     /locus_tag="PLA58_05615"
     CDS             complement(<15840..16867)
                     /gene="cobT"
                     /locus_tag="PLA58_05615"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOR62307.1"
                     /translation="MHYPTIPPIDKQAAKQAQARQNRLTKPPGSLGRLEEISIQLAGM
                     KADPMPEVSRKAVIVMAADHGVALEGVSAFPVEVTKQMVLNFLHGGAAINVLARQAGA
                     ATHIVDIGVASDFDPSLPGLWRRKIAHGTRNMAKEPAMTRVQAEQALACGMEVLAEIA
                     EKGLDVIATGDMGIGNTTSSAAIVAVMAGLPVDKVTGRGTGLDDAGLLRKIQVIEQAI
                     AVNQPDPNDPMDVLCKVGGLEIAGLAGVMIAAAARRIPVAVDGFISTAAAMIGVGLVP
                     DVRHYLFGAHRSVEIGHQVMHKHLGLTPLLNLNLRLGEGTGAVLAFHLLEAACRLLRE
                     MATFDEAGV"
     gene            complement(16881..>17702)
                     /locus_tag="PLA58_05620"
     CDS             complement(16881..>17702)
                     /locus_tag="PLA58_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOR62308.1"
                     /translation="ARAATDETGRLVRLVPAPQRIVSLAPGISETLYALGLEDKIVGV
                     TTFCDWPPAVQAKTKIGGFTNPSIEKIVALRPDLILAAADGNRKETVRQLEKLGLPVY
                     VTNPTSTSGVLKSIGHIGAITGREKEAAALVEKLRQKLRSVAVRTRGKKKPRVFFQLG
                     MEPMITAGRGTLIHEVIERAGGVNVAGNDVANYPRYSAEGIISASPDIIVFAPMVNDK
                     TFEAVYKFWRAFGEVPAVKNGKIYPIDANLINRASPRIFDAVEILSFMFHPEPKQ"
BASE COUNT         3786 a         5112 c         5027 g         3677 t
ORIGIN      
        1 tatgtgcgca gatatggaca ggtcaaaagc cgtggatttg gcgatttcgc agattgaacg
       61 acagttcggc aagggatcga tcatgaaact gggcagccac gagagcgtgg ccgacgtgcc
      121 ggccatttcc accggctcgc tgagcctcga tatcgcgctg ggaaccttcg gcgttccgcg
      181 cggccgcgtg gtggaaatct acgggccgga atcgggcggc aaaacgacgc tggcgctgca
      241 catcgtggcc gaagcgcaaa aaaaaggcgg catcggcgct tacattgacg ccgagcacgc
      301 cctcgacgtg gtgtacgcca aaaatatcgg cgtgaacacc gacgagctcc tcatctctca
      361 gccggacacg ggcgagcagg cgctcgaaat cgcggagacg ctcgtgcgca gcggcgcgct
      421 ggacgtcctg gtcgtcgatt ccgtggcggc gctggtcccc aaagcggagc tggaaggcga
      481 aatgggcgac gcccagatgg ggcttcaggc caggctcatg tcgcaggccc tgcgcaaact
      541 caccggctcg atcagcaaat cgaaaaccac cgtgatcttc atcaaccagc tgcgcatgaa
      601 aatcggcgtg ttcttcggca atcccgagac gaccaccggc ggcaacgcgc tgaagtttta
      661 ttcatcccag cgcctcgata tccgcaaaat gacttcgatc aaaaacggtc aggacgtcgt
      721 cggcttccgg accagggtca aggtcgtgaa aaacaagatg gccccgccct tccgggaggc
      781 ggaattcgac atcattttcg gcgaaggcat ttcccgcgag ggcgatgtcc tcgatctggc
      841 ggcggcaaac ggcatcattg aaaaaagcgg cgcatggtat tcctacaggg gcgaccggat
      901 cggtcaggga cgcgacaact ccaggatttt cctgaaggaa aacccggatg ttctggcggc
      961 cgtggaaaag gatgtccggg aaaaactggg catcaccgcc aaagaccagg aagaatagcg
     1021 tgaaagcgct ggacctggag gcggccaagc aaaaagcgta tcggctttta tccctgcgcc
     1081 cgcattcggc gcgcgagctg gagaaaaaac tgctggaaaa gggcttcccc aaaaccatcg
     1141 taaaagaagc actggaaaaa cttcatgaat tgaagtatct caacgacgcc tctttcgccg
     1201 gcagccgggc gcgccaactg gcggccggca agctctgggg gaaccggaaa atcacagcct
     1261 gcctgcggga aaagggcgtg gacgaccggc tgattgacga cgccatcgct tcagcgcgcc
     1321 gggagctgtc cgaagaggag gccatcgccc tgtggctgaa gaagaaggcg tccaaagggc
     1381 ggccggaccg gaaagaaaaa caaaaaattt ttcaaagcct gatgggacgg ggcttcccgc
     1441 cgggcctgat tttaaagatg ctgggaacgc cggcggagga agaagcagat gatgaaaacg
     1501 ggtgacgcga tacgggaaag cttcctgaaa ttttttgaga gcaaagggca cacacgggtg
     1561 gcgagttccc cgctcattcc caaagacgat cccacgctgc tttttaccaa cgcgggcatg
     1621 gtgcagttta aaaacgcttt tctgggcctg gaaaaccgcg gctacaccag ggccgccacc
     1681 tgccagaagt gcgcccgcgc cggcggcaag cacaacgacc tggaaaacgt gggcgtcacc
     1741 gcgcgccacc acactttttt tgaaatgctg ggcaacttct ccttcggcga ctatttcaag
     1801 gaagaagcca ttgcctgggc ttgggaatat ctcaccgacg tggtccggct ccccaaagaa
     1861 aacctctggg tgacgattta tgaaaacgac gatgaggcgt tcaaaatctg gcatgaaaaa
     1921 atgaaagtgc cggccgaccg catcgtccgg atgggagaga aaagcaattt ctggatgatg
     1981 ggcgaaaccg gcccctgcgg tccctgctcg gaaatcattt tcgatcaggg cgaagggacg
     2041 ggctgcgggc ggcccgaatg cgacattttc tgcgaatgcg accgccacct ggaaatctgg
     2101 aacaacgtct tcacgcagtt cgaccgcgat gcgtcgggca aactgacgcc gctgcccagg
     2161 cccaacatcg acacgggcat ggggctggag cgcctcacgg ccgtcattca gggcgtcaaa
     2221 agcaactacg acacggatct gtttgcgccg atcctgcgtt tcgttgaaaa gacctccgga
     2281 aaaatctacg gcaaaaacgc cgacaacgac atttccatcc gcgtgatcgc cgaccacagc
     2341 cgggccctta cctttctgat cggcgacggc attttgccgg caaacgatgg ccgcggctac
     2401 gtgctgagaa gaattttgcg ccgcgcggcg cggcacggca aaatgctcgg catcggcaag
     2461 ccgtttttaa acgaagccgc gatggtcgtc atcgacatga tgaaatacac ctatccggat
     2521 ctggccgaca aggcgccgta catcacgaaa gtgattttaa acgaagagca gcgcttcatg
     2581 gaaacgctcg acgcgggcct gcgcattctg caggaagaga cggaaacgct caaaaaagcg
     2641 ggaaagacca tcctgcccgg caagatgatt ttcaagctgt acgatacctt cggcttcccc
     2701 accgacctta cggcggacat cgttaaaaaa gacgggatca ccctggacac ggaaggcttt
     2761 gaagccgaga tggagcgcca gcgcgaacgc gcccgcggcg catggaaggg gagcggcgag
     2821 gaagccgtgg cggaatgcta cctcaaggcg tcgagctccg gcgtggccag cgttttctgc
     2881 ggttacgaaa ccgcgacgca ggacgaagcg aaaatcacgg ccattttcgt ggacggcaag
     2941 tccgtcgaaa cggcgccggc cggcgaaaag gcggaaatcg tcctggatac gacgccgttt
     3001 tacggagagt cgggggggca gacgggggat acgggttatc tcgaaggcga tggattccgt
     3061 tttcatgtgg aagacacgaa aaaacccgtt gagaatttca tcgtccatca gggcgttatc
     3121 ctgagcggag aaatcaaagt cggcgccgcg gcccgcctga tcatcgatct ggaaagacgc
     3181 agggccatcg cggccaacca ttcgggcacg catcttttgc aggcggcgct caaagccgtc
     3241 ctgggcgatc acatcaaaca gtccggatcg ctcgtcaacg ccgagcgtct gcggtttgac
     3301 tttacgcatt tttccaggat cagcgaggaa gaactgcggc gcgtcgaaac catcgtcaac
     3361 gacatgatcc gccggaatgt ttccgtgcag acggacgtct gcgcgctcga ggaagcgctc
     3421 aaaaccggcg cgacggccgt gtttgatgaa aaatacggcg cgaccgtgcg catcgttaaa
     3481 atgggcgaga tgagcatgga gctttgcggc ggcacgcatg tcgaacggac cggcgacatc
     3541 ggctttctga aaattctgca cgaatcggcc attgccgcgg gcgtccgccg gatcgaagcg
     3601 gtaacgggaa aagaggccgt tttgcatgtg caaaaaatcg aagaggaatt aaagcggacg
     3661 gcgggcctgt tcaaagccgg gcatctggaa gtgtttgacc gcgctgaaaa attattgaaa
     3721 cagtcgcggg atcaggaaaa agagatcgaa gccctcaagg gaaaactcgc cgccaaagat
     3781 tccggcgacc tgatgggccg ggtgaaggaa atcgccggcg tcaaggtgct ggccgcggaa
     3841 gtcagcatcg ccgacgcgaa aaccctgcgc gatttcggcg acaaactgcg cgaccggctc
     3901 gcctccggcg tcattctcct ggggagcaaa gcggaaggaa aagcgctgct gctgtgcatg
     3961 gtcaccaaag acctgaccgg gaaattctcc gccggaaaca tcgtcaagga actggccccg
     4021 ctcgtcggcg gtaaaggcgg cggccgcccc gacatggccc aggccggcgg caccaagccg
     4081 gaaaatctgg cggccgtatt ccctgctctg gaaaagctgc tttccgcgta gaaaaaacct
     4141 gtagcactgc atgaatattt ccatccttac cctggccgtc gtttttctcc tgatcgccat
     4201 acggcaaatc ggaagcgcca ggctccagat ctggcaaatc atgctcggcg gcgccgtctt
     4261 cgtgatcctc acgggagaaa tatcgccgcg cgcagccttt cacgccattc atttcgacgt
     4321 cattttcttt ctcttcggca tgtttatcat cgggcaggcg atggaagact ccgggtatct
     4381 cacccatatc tgccacattc tgttcggccg ggcaaaatcc gtcgatgcgc tggtgctggc
     4441 catccttttt accatgggaa tcgggtctgc ggtcctcatg aacgacaccc tcgccatcat
     4501 cggcaccccc atcatgctgc tgctggcccg aagaaaccac atttccccaa agctgcttct
     4561 cctttgcctg gcgtttgcca tcaccacggg aagcgtcatg agtcccatcg gaaatcctca
     4621 gaatctgctg atcgccctgg gaggaaacat cggaaacccc tttttgacgt tcttccaata
     4681 tctgtttgcg ccgaccatcg tcaatctctt catggcctac atcgtcctga aggttttgta
     4741 taaaaaagaa tttcaccgca cggagcttca gcccggcatc gaggacatcc ggcacgtgcg
     4801 gctggccaaa atcgcgcgtc tgtccctggt ccttgtggtt tgcctgattc tggccaaagt
     4861 agctcttgcc gtgatgaatc tttttacgcc cgtgcccttt gacttcaagc tgaccgccat
     4921 tgccctgatc ggctgttcgc cgatcctgat ctaccggttc atgtccaaga agaaaaccta
     4981 ccggtttcat atcctgagaa gaatcgactg gtttaccttg gtcttcttcg cgtccatgtt
     5041 tgttctgatg gaaagcgttt ggcgctccgg attttttcaa catctgcttt cggccttttc
     5101 gctgaacatg accggcgtcc ttgtcatgat gatcctctcc gtgctggtca gccagctgat
     5161 ctccaacgtt ccaatggtcg cgctgtttct gccgctgatg ctttccgccg gcgccggcgc
     5221 caaagagctg atggcgctcg cagccggctc caccatcgcg ggcaatcttt tcatcctcgg
     5281 cgcagccagc aacatcatta tcattcagca cgcggagaag gaaaaccgcg gcgaaagtct
     5341 ttcctttctc gaattcgcaa aggccggaat cccgttgacg atcatcaaca cggctgttta
     5401 cctgctcttt tttgagatct tctgaactga aaaacatttg acaagagccc tgctgtgcga
     5461 ttacctgcat ccaccgggtt cagtccttca agcgtgcagg gcagaatgtt gtcctgtcca
     5521 gattgttttt tttcacaggc agaaaacctc aggagaaaac catgaaaagg atcgcgaaac
     5581 gaagggggct tgcggctctg tgtctggcgg caatattcct gctcaccggc tgcgggaagg
     5641 ataatggggt tggcggggtt gccggtgacg ccggcaaggc ggtcatagcg gcaaaccagg
     5701 ctatggcgca aaaactcgac ttcagccagg accgggactt tcaggacgcc caacgcggct
     5761 tgatcgcaaa gccccatggg cagatccgcg cggcggacgg aacggtcctg gtggactttg
     5821 ccgcctttca ttttgtcgag ggcgatccgc ctcctacggt caacccaagc ctgtggcgtc
     5881 acgcgaagct caatgccagg atcggacttt tcaaagttac ggacggcatc tatcagctgc
     5941 gcggctttga tctcggcaac atgacgctga tcgaaggcaa aaccggctgg atcgtggttg
     6001 atccgctcac ctgccgggaa agcgcggctt tttcctttgc gttcgcccaa aagcacctgg
     6061 gtgaaaagcc ggtttcggca atgatcatca cccacagcca cgtggaccac ttcggcggcg
     6121 cgctgggcgt tgtctcaccg cgggaggcgg cattgcgcaa gttgccggtg atcgcgcccg
     6181 caggctttct ggaagagtcc atcagtgaaa acctgatggt gggcacggcg atgggaaggc
     6241 gttcctccta ccagttcgga aaagatctgg aacgctccgc caaaggaatg gtcgatacgg
     6301 gtctgggcaa gaccacggcc tatggcagca tcggcatcat ccggcccacg gtgatcatcg
     6361 atcagcctgt tcgggagatg ctggtggacg gtctgcgctt cgtcttttac aatacgcccg
     6421 gcgcggaagc ccccgctgaa atgacgtttg ttcttcctga aaaaaaggcg ttttgcggcg
     6481 cggagctcct atcccagacc atgcacaacc tgctgccggt tcgcggggca aaagtgagaa
     6541 atgcgctgct ctgggcccaa tatcttgacg acgcactgga atataccgcc ggttgcgagg
     6601 tgtatttcgg ccagcacagc tggccgatct ggggcagcaa tcgaatcaaa gaattcatca
     6661 cgctgcaccg cgatgtttac cgctacaccc atgatcaaac cgtgcgcctc atcaacgccg
     6721 gttacacgcc ccgggaaatt gccgacgccg tccaattgcc gaagtctctg gaatcccact
     6781 ggggggcgcg cggctactac ggcgatctgc ggcacaacgt caaggccgtg taccagtttt
     6841 acatgggatt ctacgacggc aatcccgcca acctgaatcc cctgccgccg caggaatccg
     6901 ccaaacgcta cctggagctg atcggaggag cggacaaggc tgccgatgcg gccgaggccg
     6961 ctttcaggaa gggtgaatac cgctgggcag ccgaactgtt gaacatggtc gttttcggca
     7021 aacccgatca caccaaagcc agaaaactgc tttcccgcac ctatgatcag atggcctata
     7081 tgtccgaatc ggccatctgg cgaaacagct atctcaccgc ggcgcaggaa ttgcgcgacg
     7141 ggccgccccg aaaaagcatc gaccggtccg gggcccttga tatgatggaa aacattccgg
     7201 ccgaacggtt tctcgaggtg atggcctgca gcctgaacgg accggcggcg gaaggaaaaa
     7261 actataaatt caacctggtg tttggcgata cgggggagac ttttgttctc tggatcgagc
     7321 atgccgtttt gcaccaccgg aagggaaagg cgcaggatgc acaggcaacg ctgacgctga
     7381 cccggccgat ctggatcaaa atgatggcgg gcaccgcggg aatcaaggat accctgctgt
     7441 ccgatgatct gaaaatcagc ggcagtcgaa tcgatctgat acgtttcttc gccctgctgg
     7501 agaagcccct gggcgtcttt ccgatcgtga cgccgcggta aaccattata acgcgagcat
     7561 cctgaaggag aacgaaggag gaggaagcat ggagcaccgg attcgaacgt gcgatcagga
     7621 agacatagaa gttctggtgg agatcatccg cgaggctttt caggaggtag ccgaccggtt
     7681 cgggcttacc gagcaaaaca gcccgacgca tccctccaac tgccggaccg aatggctgat
     7741 gcgggaaatg aaccggggcg tcgtctttta tgttctggaa gcgcagggcc ggccggtggg
     7801 atgcgtcgcc ctggaaaaaa taaacgacga ggtctgttac ctggagcgcc tggcggtttt
     7861 gccgcgcgag caaaaaaagg gattcgggga agcgctggta aagcacgcgc tgtcccaggc
     7921 tcgtcttttg aaggcgttcc gcgtccaaat cggcgtcatc gcggacgatt cggcattgca
     7981 aaactggtat gaaaagctgg ggtttgagga ggtggaaaga aaaaatttcc cgcacctgat
     8041 gtttgaggtt gccttcatgg cttattactt tttgtaggcg cccccctcgc aggagggggc
     8101 ttgccgcccg taccgggcgg ccgcaatggt tttctgatcc ccatggcttt cacgggaatg
     8161 aatggcatcc gtgcgcggcc gggcgatggc cgcacacgga gtggaagccg ctacttttcg
     8221 gcaagcgctt cacggtcgcc gccgctcatg ccggcaatgt acttcaccag ctttttgcgg
     8281 gattcaatat catactgcgt cgtaatggcg atctgctcca tgatcggtga tcctccgccg
     8341 tgatacgcgc cgatattgcg gacgccgccg gtcgcggagc agagcttgtc gcccagatag
     8401 cgccagaact gggcctgatc ctcggcggat attttggggc tccgcttggt gtatttcaaa
     8461 agcgcttcgc cggtttccgg attgagaaag tccttttcat gcgggaaggt ggccgtgact
     8521 cccccggata tgtcgcagag aatttccgat tcacgccaga ccgcctcgcc ggaaaggcag
     8581 cggcccacgt tgcagtaaat cgaatgggga atatacgaac cgggcccata gggaacaaat
     8641 cccttcccgg gcatgaagac cttcgggccg cccaggtcgg acgccgtgta tcccgccgca
     8701 taacccagtt ccgtcaccag aatgatttcc gccagctttt cccgaacatg ggattccttc
     8761 tggatgttgt tgcactccgc ggccagcgcc gccatgccga gaatgacgtc gccgatggcc
     8821 ggtttgcatc ccgagtagga atggcggtgg aacagcgcga agagcagcgc gttgatgccg
     8881 ccgtgctgcc attcgccggc cagaaagacg cgctcccagg gaacaaagca gttctcaaaa
     8941 atcatgtacg aatccgttga gccttccata aaaccgcgct ggtaatgctc ccgctcgcgc
     9001 agggagtgga tcgtcagcac ctgtttcaat ccgtcccagt cgccggggat tgcaaacgcc
     9061 accgcatagt ttttatcctg ctcccggagc gcccgggtgg gcacaaccag cacctcatcc
     9121 gcaacggagg cttcggagat atgcagcttg cagccttcga caacgatgcc gtctttcagc
     9181 cgctctttga tatagacgta ggaaccggga ttgggctgat ccgccggacg cagcaggcga
     9241 tcccctttga cgtccgtctg ggcacagcag ccaacgagat cttcctgctg aaaacggagc
     9301 agccatttct tgaagttttc atgatagcgg gtttcacccg gcttcacctt gtccgcttca
     9361 aaagatacgt tatagacggc gttggcggcg tcgatgccca tgcagcgctg gatgcagccg
     9421 ccgaccttct ggcagagcat gcgggtcatg tcctgcttgc gatgcaggtc ttcggtgttc
     9481 tgatggatat gggtgaaccg gttgatgcgc tctcccgtca ggtgggaaac cgccgtcatg
     9541 agatcccgat tttccggccg ttccgcctcg tcgtacgtgg tgccgatggt gttgagacag
     9601 cgcatctgca gctcatcggt gcggtcgatc aactggccgt cataataaag gttgcgcttc
     9661 attttggaga gcccggtgat gtaatcctgt ttgcttctca tgttttgatc tccttatttt
     9721 tgaaattttt tcatgttgac actaccccta tgaacccgat atagggatgc ccacaaaatg
     9781 atcttgatgt gtcgcttttg aaaagcatag cataaaaatc atggatatca ttctttattt
     9841 tatagatgtt tttattcacc tcgaccaata tctcccgatg atcatccaga gttttggcgt
     9901 gtggatctat gttcttgtat ttgttgttat attctgcgaa accggtcttg tggtaacgcc
     9961 ggttcttccc ggcgactctc tgctttttgc cctgggaacg ctggccgccc tgggcgcgct
    10021 gcacatcgaa gcgcttttga ttctgctttg catcgccgcc gtcgccggag acacggtcaa
    10081 ctatgccatc ggccattaca tcggtccgaa ggtttttcat tacgaagaaa gccggttctt
    10141 taaaaagaaa tacctgatca aaacgcacga attttacgaa aggcacggtg gaaagacgat
    10201 tatcatcgcc cggtttatgc cgtttatccg cacctttgcc ccgttcgtcg cggggatcgg
    10261 agcgatgagc tatgcccgct tcattgtata caatattgtc ggcggtgtat cctgggtggc
    10321 catcttcctc ctgggggggt actttttcgg caatatcccg gccgtcaaaa cgaattttac
    10381 cattgtcatt gtcgcgatta tcatcatttc cgtgatgccg gggttcattg cgtacttccg
    10441 gcaaaaatat cttgcccgga aagcagggag tgaatcatga tcggctgttg tccaaatacc
    10501 cttttcgctt gcctgagatc gttttgtata aatctgatga tattaaaatt tgccgtcatt
    10561 gtggccgccg tccttttgct tttgccggcg cacgccgatg ctaaaaatat tgtcaaggca
    10621 ggcagcgaca ttcttgtgga agaaggacag accgtggaca atgtcgccgt catcggcgga
    10681 cagatcacgg tcagcgggct tgtggaaaac aatgttctgg cgatcgccgg ctcggtggtt
    10741 ttgaccagca aggccgttgt ccggggcaaa gtgattgtcc tgggcggcat tgtggccaga
    10801 ggcagcggtt ccactgtttt cggcgacatc acggaagtca acgctgccac cctgtcggaa
    10861 gcttttcagt cggccatgcg cggggagctg gaaggctggt cgctgatttt aaatgtaatc
    10921 tccatttgtt ttttcgccat catcctgatg attgcgctga ttatgacgct gctcgttccc
    10981 cgccgcctga ccatggtgac cgatgccatc ggagcaaacc ggctcaaagc attctttttg
    11041 ggcattttgg cgacgctgat gatcgcgccc ctgtttatgc tgctggcgat ctccattatc
    11101 ggcatttttt tgattccgct ggctttcacg gcgcttctgc tggccgtcat tatcggcttc
    11161 atcgcgtccg gcgcgattct gggcaattgg atcatttcca gaagctttcg cggacacagg
    11221 aaatctctgg tgaaggaaac gctggtaggc ttgtgtctgt tgtggctgct gggctggata
    11281 cccctctata tcggaatgat catcaaggtt ttcgccatca ccatcgggct gggcggcgtc
    11341 ctgttgagcc tcagccgcaa aaccgtcagg ccgatagaaa atcaagcgcc gtccgattga
    11401 accggtccgg gtcctgaaga tgcagggcgt gtccgcatcc ttcaaacgca accacgcggg
    11461 catcgggcat ctgttgttgc atgtagtcgt agttttcggg cggataataa ttgctttccg
    11521 tcccgtacgc cagcagacag gggacggtga tgtcttttaa aacggctcgg taatcctgcg
    11581 tcgccatcgc gatccagaaa gcggcaatga cacaggcgtt gttcttccgg gcctgctcgt
    11641 aaagccacgg caaatcgctt ttccccttga agtccccctc cgcgttgaat tccatttttt
    11701 cattgtagag cgatttattc aaaatccgcc gggcgacgac ccggctgtaa gcctcccagt
    11761 ctttgagcat gaccgacaaa aggaaaagat tgtcttcatg tccgaaatcc cccggcttgc
    11821 ggctgaagac gccgggcagg ccgcaggacc agtcgtccgc cttcatcagc ctgggcgtca
    11881 tgtcgatgat cacgatttta tcaatcgacg cgcaggaata gttcctgatg tattcgaaaa
    11941 tgacctgagc gcccatcgac cagccgacaa gcgacgcttt ggaaatatga agctcggcga
    12001 gaagctgctt caaatcctcg gccaggcgcg aaagggtcca gtggtcgctt ccggcttccg
    12061 attggccgtg gccgcgcaaa tcgagcgcga tcaccgaaaa atgcctgctc agctcgggaa
    12121 tctgtttccg gaaaaaaaga gagttggccg tcaatccatg aatcagcaca accggctttc
    12181 cgctgccgct tttttcataa taaaggcgaa ccccgtcatc cgctgtaaaa aaagccatgg
    12241 tggcctcctg tacgtcttga tggcctgttt tccaaagata cccttttcgc ttgcctgtga
    12301 gcgttttgta taaatctaag gcttgcgaaa ggctttgaca aaactcgcct tcggctcaaa
    12361 cagttgtcaa aggctgatct ttcgcttcgc caagattgta ttacaaaaca ctcccaatgg
    12421 ccgctcaaag ggatatcttt gggcaaccac ctattgtcag ctttccccta ctcaggccat
    12481 ccttttcatc gcgtccacca gctcccgaac cgcggcggcg gatttctcca gggccttttg
    12541 ctcttcaggc agcagtcgca gcgaaataat ttcttcgatg ccgcttttgc ccagtttgac
    12601 cggcacgccg acaaacaaac cggaaatgcc gtactcccct tccaggtaag cggcgcaggg
    12661 aagaattttc ttcttgtccc tgaggatgga ttccgccatt tcgacggccg ccgaagccgg
    12721 cgcgtagtag gcgctgccgg ttttcaggag cccgacgatt tcggccccgc cgtttcgcgt
    12781 gcgcatggcc agggcttcaa tgcgctccgg agacatcagc tccgtaatgg gaatgcccgc
    12841 cacggtggaa aagcggggca gcggaaccat ggtgtctccg tggccgccca gaaccatcgc
    12901 atgaacgttc tccacggaga cgctgagctc catggcaatg aaagcccgga agcgggccga
    12961 atccagaacg ccggccatgc cgatcacgcg gtttttggga aagccgcttt cctccatcgc
    13021 cacgtggcac atggcgtcga gcggattgct gacgataatg agaatggcgt caggagagta
    13081 tcgaaccgcc tggcgtacga cgtttttcat gatgccccgg ttggtcgcca gcagatcgtc
    13141 ccggctcatg cccggtttgc gcggaaggcc cgccgtgatg atcacgatgt cggaaccggc
    13201 ggaagcctcg taggtgttgg cgcccgcaag atgcgcgtca tgcttttcaa tcggcgcggc
    13261 ctgggccaga tccagggact tgccctgcgg tacgccttcc acgacatcga tcaataccac
    13321 atcggccagt gctttctccg ccagccgctg cgccgccgtc gccccgacat tgccggctcc
    13381 tacaaccgta actttctggt tcatctctta tgctctcccg atatttttta atgcaaaatt
    13441 cgtcgatgga tcgttttgcc cctataataa aaccgaaaat taagcaacct cgcgcccctg
    13501 cttatttgat caccatcgga attcccgcga ccatcaggat cacgcgcgcc gctttctgcg
    13561 cgagggcctg gttggcgcgg cccagcgcat cgcggtagag gcgccccagc ggatagggag
    13621 gcacgagccc caggccgacc tcgttggaca ccaccagcca ctgcccgccc agccttgcct
    13681 gcgcggcaat aagctcatcc atttcggcgc ggactttttc catgacgata tcttcggagg
    13741 tgttttcagg aagggacagc aggatgttgt tgaccaggag cgtgatgcaa tcgacgatga
    13801 ttacgccggc cgcggttgtt tgaaggctgc agccgatgtc catcggaagt tcgagcgtct
    13861 gccacccggc cggccgcgag gcccggtgcc ggcggatcct ttcagccatt tcctcatcgc
    13921 ccgcgcaggc ggtggcgata aaaagcacag gctttcccga ctcgcgggcg gtttgttcgg
    13981 caaacgtgct tttcccgctg cgcgcgccgc ccaggatgag cgtcatgccc ttttccggaa
    14041 tttcagcaat catcattttc cccttaaaga acgatggcgc ctgttgtccg cgccggtttt
    14101 cgccgtctcc cggtcgggtt agcgcaggtt cgcatgcatc gccgcgtcga tcgccgcgcg
    14161 ggaagtcaaa aaatcagcct cggaaaaaac ttccatcgtc aatacgccgc ggtagtcgct
    14221 gccgaccagc tcgtccagga cggctttcag cttctcccgc ggcgcttttg ccgcagagcg
    14281 gtgatcgcgg tcctcaattc cgtgaatgtg aatcacgcgt gtgcgcggca gcgctttttt
    14341 tagatacgcg accggatcgc agccgtcaag ccacaaatga ccgacatcga ccgtccggct
    14401 gacggaaatc cgatccagca ccggatcgta aaaatccggc ggatagcctt ccagattttc
    14461 cacggccaga cgctgtgggc cgcccgccca tttgccgacc aactcaagcg caaggacagc
    14521 ctggtcccgc cagcgctcca tactcgccgg ctctgagcgg ttcaaaacgt cttttccgtc
    14581 aaggtgaaga acgtaggccc agggatcgag cgcccgcgtg cgttcgatga cacgtcgggc
    14641 cttatccagg gaagcgtggc gccggaaacc gtcatcggtc aggcgcaaat ccagcggcag
    14701 atggaccgtg taggtcaaat cacaaccgcg ggcgatggcg acaagctctg caatctccga
    14761 atttgccggc aggttgttcg ggccgtcatc gacttcgaac agcaccagtt ccacgtcccg
    14821 cactttcccg gccagatagc ggacattcgt cagaatgtcc gcggggacga tgtaggaggt
    14881 tgtgcccagt cgaaaaggat aggtcatgtt tcccctttgg aacaaggggt cttaccaccc
    14941 gcaggggtgg caacctcttg ttctgttgtg tgtatgccga ggatttacaa cgcacgacac
    15001 gagatcgtga aaaccaatag caccgctgtt tcggtcactt caaccagcgc gcccaggacg
    15061 tctccggtca ccccgccaat gcgcttccag gccagtccca ggaccgcggc ggccgcgatc
    15121 agcgcggcga ggacagccat ccagccctgc attcccagca gaaacgcgag gccgatcggt
    15181 agaatcgccg cgaaaaaaat cgagcccgtc tgaagccctg ctgcaaaatc agcgcccatc
    15241 ccgccggggc gcgcctgcgg cagccgtgag acgggcagaa gaagccagcg gccaagcgtc
    15301 gcggcaagga caatggacaa tccggaagaa ggcggcatca gacccagcgc agcagctttc
    15361 agcatcagcg cgaagaacag cccgatgacg ccgaacgcgc ccaggtgagg gtctttcatg
    15421 atctccagac ggcgttcccg tgaagccgac cccaaaaggc cgtcgcagca atccgccagt
    15481 ccgtccaggt gcaggccgcc ggtcaggcca acccagacgg caagcgtaag tacggaggcg
    15541 acaagcgcag gaaagatcag gttcagtcca aaccaggcca gccagacaag accgcccacc
    15601 gcaagaccga ccaccggata ccagaaggcg gcgcgaccgc tgtcgcccgg ccgccagttt
    15661 tccggcacac cgacaggcag cgtggtcagc aatccgaatg ccgtgcgcaa attccgcata
    15721 ctgttcctct tccccttcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    15841 ctcccgcttc gtcaaacgtc gccatctccc gcaggagacg gcaggccgct tccagcaggt
    15901 gaaacgccag caccgccccc gtcccttcac cgaggcgcag attgagattc agaagcgggg
    15961 taagaccgag atgtttgtgc ataacctgat gcccgatctc caccgaccgg tgcgcgccga
    16021 aaagataatg acgcacatcc ggcacaagcc cgacgccaat catcgcggca gccgttgaaa
    16081 tgaagccatc caccgctacg gggatgcggc gggcggcggc ggcgatcatc acaccggcaa
    16141 gaccggcgat ttccaggccg ccgactttgc aaagcacatc catcgggtcg ttcgggtctg
    16201 gctggttgac ggcgatggcc tgttcgatca cctgtatctt acgcaggagg ccggcatcgt
    16261 caaggcctgt accgcgtccc gttactttat caaccggtaa tcccgccatt acggcaacaa
    16321 tggccgcaga tgaggtggtg ttgccgatgc ccatgtcgcc tgtggcaatc acatcgaggc
    16381 ctttttcggc aatttcggcc agcacctcca taccgcaggc aagcgcctgt tcggcctgga
    16441 cgcgcgtcat ggccggctcc tttgccatat tgcgcgttcc atgcgcaatt ttccgccgcc
    16501 agagcccggg cagggacgga tcaaaatcgg aagcgacgcc gatatcgaca atatgcgtcg
    16561 cggcgcccgc ctggcgcgcc agcacattga tggctgcgcc gccgtgaaga aaattcagca
    16621 ccatttgttt tgtgacttcg accggaaagg cgctgacgcc ctccagcgcc acgccgtggt
    16681 ccgcggccat gacgatcacc gcttttctgg aaacctccgg catcgggtcg gccttcatgc
    16741 ccgccagttg gatggaaatc tcctccagcc ggccgaggct tcccgggggt ttggtcaggc
    16801 ggttttgcct ggcctgtgct tgtttcgcgg cctgtttgtc aatcggggga atcgtcggat
    16861 aatgcatatg gtatcctttt ttactgtttt ggctccggat ggaacatgaa cgaaagaatt
    16921 tcgaccgcgt caaaaatacg cggagaagcg cgattgatca gattggcgtc gatcggataa
    16981 atcttgccgt tttttaccgc cgggacttct ccgaacgccc gccagaattt gtaaacggcc
    17041 tcaaacgttt tgtcattgac catcggcgca aacacgatga tgtccggcga cgcgctgatg
    17101 atgccctccg cgctgtagcg cggatagttg gcgacgtcgt ttccggcaac gttcaccccg
    17161 ccggcccgtt caatgacttc atggatcagg gttccccggc cggcggtgat catgggctcc
    17221 atgcccagct ggaaaaaaac gcgcggcttc tttttgccgc gtgtccggac ggcaacagag
    17281 cgtaatttct gccggagttt ttcaaccagc gcggcagcct ccttttcccg gccggtgatg
    17341 gcgccgatat gcccgatgct tttcaaaacg ccggacgtgc tggtcggatt ggtgacatag
    17401 acgggcagcc ccagtttttc cagttgcctc acggtttcct tccggttgcc gtcggcggcg
    17461 gccagaatca aatccggccg gagcgcgaca atcttctcta tggaaggatt tgtaaaaccg
    17521 ccgattttcg tcttggcctg gacggccgga ggccagtcgc aaaaagtggt gaccccgacg
    17581 attttatctt ccaggcccag cgcgtaaagc gtctccgaaa ttccgggggc gagggaaaca
    17641 atcctctgcg gcgcagggac aagccggaca agacggccgg tttcgtccgt cgcggcgcgg
    17701 gc
//
LOCUS       DAOSRM010000041        17580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3348_length_17580_cov_118.434009, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000041 DAOSRM010000000
VERSION     DAOSRM010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 17580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17580
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3348_length_17580_cov_118.434009"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            443..1459
                     /locus_tag="PLA58_05625"
     CDS             443..1459
                     /locus_tag="PLA58_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62309.1"
                     /translation="MKRILILSLAVICLAAANAQAVVITGSGTTKEEAIHNGLRDAVE
                     MYTGTLVYGVTDVENYTFQKDQIVASSLGYVKHYRIIRTSRVDDLIHVVMDVTLSEDK
                     IDRILRDNVDLMTIEDVLKDYNNVTQRQDQMKKLMEMLKILADRPLSEKYSIIYTGYE
                     IKRIMPDKVNVEIHAKIYANPFFMRANREILRHLSDPTGDSPDVWRIGTNYRIEYGKL
                     KNDSYFITNQAKVRDELKVYVTINDEKVGDKFYRLRDNLFVVWDSVAFTKAFVRLFPK
                     TFWEKYNDKETKFDKKWNNYAIRKSKIIPEDGLPMKFKYTIDSEQVKDLRDLKLKLEY
                     YEKN"
     gene            1473..1925
                     /locus_tag="PLA58_05630"
     CDS             1473..1925
                     /locus_tag="PLA58_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62310.1"
                     /translation="MARKHRLLRTLLLIFSVIVCITINVTDCHGDNYDQNIKKAVQDC
                     RRSYNATGMSLMRGRVDKCYQNALRRHDRKKFDYCAAFDLSAYFVDDMMVRQIHCPPF
                     EGFDSASVSKRIEGGLTALGYDKDRRKTEVKRLADTTVKWFKEIFETE"
     gene            complement(1977..2525)
                     /locus_tag="PLA58_05635"
     CDS             complement(1977..2525)
                     /locus_tag="PLA58_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOR62311.1"
                     /translation="MPENTIIDSAMSFAEAIEGTTAPMEVIDSLSMIDVLYYSFDGQR
                     HQGQMLVDARLEDDVYEIFLLIEKLKFPVGKVIPIVAYKWDDEASMADNNSSSFNFRV
                     IADTNKLSLHSFGRAVDINPVQNPVIYPAGLIAPEGAVYRPQNKGAFTGDHPVVQEFL
                     RRGWHWGGNFDQPKDYHHFEKT"
     gene            complement(2639..>3724)
                     /locus_tag="PLA58_05640"
     CDS             complement(2639..>3724)
                     /locus_tag="PLA58_05640"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOR62312.1"
                     /translation="ESFNAGFPVALRLPGMTKKALAASPQISDRQRVSSASLTSNRHF
                     AEFPGERSKTLPALCRALVESQKKSWPAYATACRGLASAQTRKLSCGDYSVTLQYNPA
                     RAISSGAAVDAESIRKRPCFLCAENRPARQSGILYRDDYQILCNPAPIFERHFTVAAL
                     SHLPQDIRSSLDALLDIAHDAAPGHAVFYNGPACGASAPDHLHFQMIPSRALPFLNEL
                     EKGSFPMASSTVLHRPAKSFDRSVLLLKSDQARLLKEHLTRFLTAARTSLATQEEPLL
                     NVFCAYGKRGWTITVFLRRKHRPDAYFAPGKSGIFVSPGAIDMAGVVITPRLSDFQRL
                     TADTVRGIYREVSLEEAALNKIIADLS"
     assembly_gap    3725..3824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3825..5361)
                     /locus_tag="PLA58_05645"
     CDS             complement(<3825..5361)
                     /locus_tag="PLA58_05645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR62313.1"
                     /translation="MKTSVYQWGRLLLWSAPDEIQAALRRPSGSGPFRPVKRPSTFFK
                     NLPADYLLAMDAQKDVLCGPPELRRLLAVARDTGAGLVYSDYVEKINGRFAGHPVNDY
                     QPGSLRDDFHFGPFFILSVRAALSAIEQYGALPGDPDMAFYDLRLRISLEHDLIRVPE
                     SLYTVSAKERVPSKKPGGKSEAHFSYVAGENLLRQKKLEKIATSHLRRLGARLPSRPA
                     ANRQPEEILWKASIVIPVRNRRKTIADAIKSALAQKTDFPFNILAVDNFSTDGTTELL
                     KDFAARYPHVHHLVPFRRDLGIGGCWNEAIFSPHCGRYAVQLDSDDLYESPGTLQTMV
                     DALRRGPCAMAVGSYTLVNERGKKIPPGLIDHREWTVRNGHNNLLRVGGIGAPRAFDT
                     AVLRRIGFPNVSYGEDYAVALKLSREYRIGRVYQSIYLCRRWKDNTDAELSVDKQNRN
                     DFYKDRLRTLEIKARQQQNRRERFQTTSGSGKTVPDKQPSPANANQIAARKIEDKNQK
                     EFRH"
     gene            complement(5378..5911)
                     /locus_tag="PLA58_05650"
     CDS             complement(5378..5911)
                     /locus_tag="PLA58_05650"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOR62314.1"
                     /translation="MKKPRGLSAVKLSLLSLSCVLLLSCGYGFAPQGEYIDKRIVNIY
                     VAPFVNKTAEAEAENILRNAFIDQIIQSNRFKSAPTLEQADAIINVNILSITTGVLSY
                     RNNILAAEERMSITLEASLREKDSGKTIWSSQNVVGTSDYKLQDNVNPKPARQQALAK
                     LAKDTAENTFNLMMSDF"
     gene            complement(5925..8504)
                     /gene="leuS"
                     /locus_tag="PLA58_05655"
     CDS             complement(5925..8504)
                     /gene="leuS"
                     /locus_tag="PLA58_05655"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOR62315.1"
                     /translation="MKYDPIAIEEKWQKKWEEEKTFKVVEDPNKKKYYLLEMFPYPSG
                     KIHIGHVRNYTIGDVVARYKRMKGFNVLHPIGWDAFGMPAENAAIEHKIHPSRWTHEN
                     IDHMKKQLKRMGFSYDWDREVATCEPQYYRWEQLFFIWMVEKGLAYKKRSSVNFCARC
                     DTVLANEQVEGGLCWRCGTEVTEKTLDQWFFKITAYTEELLEYCDRLPGWPERVMTMQ
                     KNWIGKSYGCLVDFPMAEGNGVIQVFTTRQDTLFGATFMLVAAEHPLVTELAKGKPCE
                     KNVRAFVEKVKKQDKLMRTSEYYEKEGLFLDSYCLNPLTGYKMPIYAANFVLADYGTG
                     CVMAVPTHDQRDFEFAKKFNLELVVVISPKDRTLDAATMTEAYVDEGILVNSGPFNGM
                     ENTKVLEAIADYLEAEGKGKRTIQYRLRDWGISRQRYWGAPIPMIDCKKCGIVPVPEK
                     DLPVILPENVIFSPEGGSPLAALPDFVNTTCPQCGGPAARETDTMDTFVESSWYFDRF
                     CCPGYNTKPGLDRKALDYWMPVDQYIGGIEHAILHLLYARFYTKVLRDFGVIGVDEPF
                     TNLLTQGMVCKETMKCPEHGYLFPEQAQSGKCQFCGKDVIIGKTEKMSKSLKNVIDPD
                     FLVKTYGADTARIFCLFASPPEKDLEWSEQGVEGSFRFLGRLWRIFDEYVEDIRGAAP
                     AAGPLELTDDVKALRRKTHQTIRKVSQDIEDRFHFNTAISAVMELVNVLYSVKHPTKD
                     NRTALSVVREALETAILLLTPIVPHITEELWERLGKDKPAADMPWPDFDPAVASEEEM
                     TIVIQINGKLRSRMTVPVDCDAEKIKADAQADEKIAAFIGKNKILKVIYVPKKLVNIV
                     VAP"
     gene            complement(8617..9813)
                     /locus_tag="PLA58_05660"
     CDS             complement(8617..9813)
                     /locus_tag="PLA58_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR62316.1"
                     /translation="MIPEHTEPSPAGLLKLAGFAMGLFAVQTFWGFTWATLPLYLKEL
                     TGSNAGTGVILSTTGITGLFFPVLAGALSDRIRTPLGRRRPLIVAGWLLAWSMVLLLT
                     QIHSLAAAIPVIFLAYAGFFFAIGPYFALLPDIIPAAQRSFASGVMFLIGGTGILSYL
                     LFAARWWEVSPTRPFVWVLIAVPAAVAVMCLSVKESVPPQGKTRLRLFRELSGRKNLL
                     RFLSGMTLQWISLWMVSAFFVITCMALFSLSVEQAVKAFFVLNASFVGFALPGGLLGA
                     KLGLKQTTIGGVAALIVCFCVIPFLQTYESLLFLVIIAGAAYGTVLAVSYPFFLSMIP
                     KDQTAGFVGLYQACQNGTLLLGPAIAGGLIDSFGYTTLFFGAAAVMLAGMILYITVRR
                     SEEQEP"
     gene            complement(9864..10847)
                     /locus_tag="PLA58_05665"
     CDS             complement(9864..10847)
                     /locus_tag="PLA58_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOR62317.1"
                     /translation="MNRDIRQFVNHHLGAVEFQYDPILKGGSDRTFYRVRVPGGSTFI
                     FMEYGTDVEENAYWAEINRFMTEIGMPVPRIIAFDFRKRFMLLEDLGDADLYSLRAAP
                     WRKRRRHYLTALSGIHRLHRTPLSDVPASLKLCRGYDRSLYAWEHAYFRENFVEAVCG
                     LSLSGPQERDWAEEMEALIDRLQKIPECLIHRDFQSQNIMIRNDRPVFLDFQGMRTGN
                     LFYDLGSLICDPYVSFSSSERNELVAFYYRIMEPDYSFEKFTDYFWEGAAQRLMQALG
                     AYGFLGLKKNKPDFLRHTAGGIRNLAAAASQTGTMPVLKELARTCANKLSA"
     gene            complement(10844..11578)
                     /locus_tag="PLA58_05670"
     CDS             complement(10844..11578)
                     /locus_tag="PLA58_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOR62318.1"
                     /translation="MNRCPSDSAEPKIRTAFILGAGLGTRLRPLTQHLPKPLLPVGGR
                     PIITYAMEHLRAAGVERFIINTHHCPEKYREAFPESRWGNIPIIFRHEPVLLDTAGGI
                     KNIEDLIGQDRRIIVYNGDIITNLPLEPLLKRHAELCTEATLALRSRGPLTNVRIDSR
                     GFICDMRDALHNPGVQSCLFAGIYVLETAFLRRLSAGKIESIVLPLVESIRQNPRSVG
                     GIVIDEGFWHDLGTMEEYTTLRDQTL"
     gene            12055..12411
                     /locus_tag="PLA58_05675"
     CDS             12055..12411
                     /locus_tag="PLA58_05675"
                     /inference="COORDINATES: protein motif:HMM:NF037472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HOR62319.1"
                     /translation="MKKRSVAVLLIVLVLGAYPARAMNGHDLADGMKSYLKAEQDYAA
                     ADYLLAGNYMGYIQGVAEATVSDYSLPSGVTPDTLYKIVADYLQKNPEKWDKPASFLV
                     RSALIAAYPKYLIRNK"
     gene            12429..13127
                     /locus_tag="PLA58_05680"
     CDS             12429..13127
                     /locus_tag="PLA58_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOR62320.1"
                     /translation="MKKLMLAGMLLLLFSGCSPAQKISPEERFKKSFPRHSYETITQT
                     SAKGVYEVYNGSQIYYYLPDGDMIFVGSMISKDGTNLTEESNGKKMTSKMAGLSLDKA
                     LKIGEGKTSVVEFIDPNCHYCRQSHAYFAGRMKEVTLYVFFYPLSRDSEDKIRHIICA
                     ADKSGEYAKVLSGELDEKKPLNLCGDKDAEALIAAHREASAKIGVRATPLFYIKGRVV
                     PGFDRPVIEALLKK"
     gene            complement(13143..15035)
                     /gene="metG"
                     /locus_tag="PLA58_05685"
     CDS             complement(13143..15035)
                     /gene="metG"
                     /locus_tag="PLA58_05685"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOR62321.1"
                     /translation="MSRPFYITTPIYYVNASPHIGHAYTTIVADVLARFARMTGRETF
                     FVTGTDEHGDKIAEAAQKAGITPKAYADGVSAQFRALWPELAITNDYFIRTTDENHTE
                     TVRRILQKVYDAGDIYFGSYEGFYCVGCERFYTEKELVEGKCPDHQTEPEHRKESNYF
                     FRMSKYRDWLVRHIQENPDFIRPERYRNEVLAFLREPLEDLCISRPKTRLEWGITLPF
                     DDQYVTYVWFDALINYVTAIGYPDGPNFKKFWPEAQHLIAKDILKPHGIYWPTMLKAA
                     GIDPYRHLNVHGYWNSDSSKMSKSLGNVVRPLDLKDKYGLDAFRYFLLRDMVFGLDSN
                     FSEEAFVARLNSDLANDLGNLVSRSVTMAVKYCDGKIPAPPDAALEVRLPELAQKTVA
                     EVEACFADLSLHKALMAIWELVGAANKHIVENEPWNLAKDPANREKLLAIMYRLLEVL
                     RSVAILIAPFMPQASEKILGSLGLEETAKFNLDTIRRGEPLPAGAALSRGESLFPRVS
                     AEKEAPPPKKPAVELKPEIDYDEFSKVDLRVAKILAAEAVPKSNKLVKLRIDIGEERT
                     IVAGIGKDYKPEDLVGKSIVIVANLKPAKLMGVESHGMLLATDAPEGLTLIGFDREPK
                     TGAKVR"
     gene            complement(15052..15831)
                     /gene="ricT"
                     /locus_tag="PLA58_05690"
     CDS             complement(15052..15831)
                     /gene="ricT"
                     /locus_tag="PLA58_05690"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOR62322.1"
                     /translation="MQINILGVKFKKEGRIYHFDAADLILHKNDQVLVNTDNGVALGT
                     VVTDVRHCDASDPPPNLKNVIRKVTADDLRVREELELLEEEAKKYCVGKIAEKGLSMK
                     LITVECLFDRSKMIFYFTADARVDFRELVKDLVSRFKTRIELKQIGARQEARIVKGIA
                     VCGRTVCCAGILQNLDRVTVKMAKQQSMSLNPEKISGLCGRLMCCLSFEHEGYADARK
                     SAREAARIEPVREVPEGSEPPHQIRQRPNGSRENKKKRKAV"
     gene            complement(15851..16831)
                     /gene="holB"
                     /locus_tag="PLA58_05695"
     CDS             complement(15851..16831)
                     /gene="holB"
                     /locus_tag="PLA58_05695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOR62323.1"
                     /translation="MSFANIYGHTKQISMLQKAMAQKRVGHSYIFSGLSAIGKKTLAL
                     EFAQAQNCENAALRQDACGQCPSCRKTTHGSHPDIHVLQAQTQFIRIDDVRGIQDQMT
                     FRPLEGGKRVFIIDEADKMNEPAANALLKTLEEPSADNILILVTARPFRLPQTILSRC
                     RHLRFNPLAAGDVARFLTEQKRMEPAHASALASLSGGSIGRALELDAEDMIAFRAGLS
                     GLLRDAGRRDPMSLIVLASFLGQDKKHTQQGLSILHTYFRDALVYRETARASMLLNAD
                     DLPVVESLAGRLEGDDLLHNIAVVEKSRQSLDMNANKSLTLDAMAFKLHL"
     gene            complement(16854..>17580)
                     /gene="tmk"
                     /locus_tag="PLA58_05700"
     CDS             complement(16854..>17580)
                     /gene="tmk"
                     /locus_tag="PLA58_05700"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOR62324.1"
                     /translation="RFLPSVEMTKMAVIQSSHPMTPGEPFMNRFITFEGVEGSGKSTQ
                     IQLLGDYLTGEKIPVVLTQEPSGTPIGRKIGDILFHRGHFAMCPETELLLFLAARAQH
                     VREVVLPALQSGKTVLCDRYSDATFAYQAAGRGLDPAFVAAANDYCAMQVRPSLTLLF
                     DLPVETGLERAGRRDARLTNPAAADRFEREKLDFHNRVRQAYLNLYTAEPERFRLIDA
                     SGSVEEIADQVRGQVMDFIHKQR"
BASE COUNT         3758 a         4609 c         5150 g         3963 t
ORIGIN      
        1 ttcacgggtg atgatggaca tggcttgatc ctccctttgg ttgccatatt aaccatgaaa
       61 agggcgcttg tcaagataaa tcatcaatat attcaatagg ttaggagtgt tttcatatag
      121 aagtaacgat ttctatatga aaccggccga atatcaaaaa ataataccga agaccttgat
      181 atctttggtc tttacaaaga tgatggccaa aagtgaaaaa caaaaaattc gaaaagattt
      241 tttctggatt ccggctaaaa atctgccggg atgaaggagg ggagattgtg ccttccccta
      301 aagggatttt tttcatatag aaatagtatt tccataggaa aattatcata tctatatgat
      361 tatattgata atttatattt actttttgcc cggctttgtg tatcctgcct gcatcattac
      421 gggtcaagcg aggttccggg aaatgaaaag aatccttatt ctgtcgttgg cggttatctg
      481 tctggcggcg gcaaacgcgc aggcggtggt cattaccggg tcgggaacaa ccaaagaaga
      541 ggcgattcat aacggtctgc gcgatgccgt cgaaatgtac accggcacac tggtttatgg
      601 ggtaacggat gttgaaaact acacatttca gaaggatcag atcgtggcat cttccctggg
      661 ctatgtgaag cactaccgga tcatccggac atccagggtg gatgatctga ttcatgtagt
      721 aatggatgtt acgctttctg aagacaagat tgaccggatt ctgcgggaca atgttgattt
      781 gatgaccatc gaggatgttc tgaaagatta taacaatgta acccagcggc aggaccagat
      841 gaaaaagctg atggaaatgc tgaagatcct ggcggaccgc cccctaagcg agaaatactc
      901 catcatctac accggctacg aaatcaaacg gatcatgccg gataaagtga atgtagaaat
      961 ccacgcaaag atttatgcca acccgttctt tatgagagcg aacagggaaa tccttcggca
     1021 tctttccgat ccaaccggcg attctccaga tgtttggaga atcgggacga attatagaat
     1081 tgaatatggc aaattgaaaa atgacagtta ctttattacg aaccaagcaa aggttcgtga
     1141 tgaactcaaa gtatatgtca caatcaatga cgaaaaagtt ggggataagt tctatagact
     1201 tcgagataat ctgttcgtgg tctgggattc agtagctttc actaaagcct ttgtcagact
     1261 gtttcctaaa acgttttggg aaaaatataa tgacaaggaa acaaagtttg acaaaaaatg
     1321 gaacaattat gcaatcagaa agtcgaaaat tatcccggaa gatggactgc cgatgaaatt
     1381 caagtacacg atagattcgg agcaagtgaa agacttgaga gatctgaagc tgaaacttga
     1441 atattatgaa aaaaattgag gagatttcgc ttatggcaag gaaacacagg ctgctgcgca
     1501 cgctactgtt gatcttctcg gtgatcgttt gcataacaat aaacgtgacg gattgccacg
     1561 gagataatta cgatcaaaat atcaaaaagg cggtccagga ttgcaggaga tcctataatg
     1621 caactggcat gagcctgatg cgcggcaggg ttgacaagtg ttatcagaac gccctgcgga
     1681 gacatgatag gaagaagttt gattactgcg ccgctttcga tctttccgcg tatttcgtcg
     1741 atgacatgat ggttcggcag attcattgcc cgccgtttga aggttttgat tcggcatccg
     1801 tttcaaaaag aatagaaggc gggttgacgg ctctggggta tgacaaagac cggcgcaaaa
     1861 cagaagtcaa acggcttgcc gacaccactg tgaaatggtt caaggagatt ttcgagaccg
     1921 aataaagact ttcatctggt tgaaccggag gcatgtcttt tttcatattt tgatccttac
     1981 gttttttcga agtgatgata atccttgggc tggtcgaaat ttccgcccca gtgccagccg
     2041 cgccggagga attcctgaac aacggggtga tcgccggtaa atgctccttt gttttgcggg
     2101 cggtaaaccg cgccttcggg cgcgatcagg cccgcgggat aaatcaccgg attctggacg
     2161 ggattgatgt ccacggcgcg gccgaaagaa tgcagagaca gtttgttggt gtcggcgatg
     2221 acgcggaaat tgaaagagga ggaattgttg tccgccatcg aggcttcatc gtcccacttg
     2281 taggcgacga tgggaatgac tttgccgacg ggaaatttca gcttctcgat caggagaaag
     2341 atttcataaa cgtcgtcttc gaggcgcgcg tccacgagca tctggccctg gtggcgctgt
     2401 ccgtcaaagg aataatacag gacgtcgatc atggacaggg aatcgatcac ttccatgggc
     2461 gcggtggtgc cttcaatggc ttcggcaaag ctcatggcgc tgtcgatgat ggtgttttca
     2521 ggcatttcaa aaactccgta ataaattcac cctcccatca agggagggga gaaaaaaccg
     2581 acggggttat tttccaccct ttttcgccgt tttttgtctc acagcacgcg gtgcggggct
     2641 atgagaggtc ggcgatgatt ttgttcagcg ccgcttcctc cagcgagact tcccggtaga
     2701 tgccgcggac ggtgtcggcg gtgagccgct ggaaatcgga aagccttggc gtaatgacga
     2761 cgccggccat gtcaatggcg ccgggcgaga cgaagatgcc gcttttcccc ggcgcgaaat
     2821 aggcgtcggg ccggtgtttc cgcctcagaa agacggtgat cgtccaaccg cgcttcccgt
     2881 aagcgcaaaa aacgttcagc aggggctctt cctgtgtcgc aagcgacgtc cgggcggcgg
     2941 tgagaaatcg cgtcaggtgc tctttgagga gcctggcctg gtccgacttg aggagcagga
     3001 cactgcgatc gaagcttttc gccgggcggt gcaaaacggt ggatgacgcc atcgggaaag
     3061 accctttttc cagttcattc aaaaagggaa gcgcgcggga tggaatcatc tgaaagtgca
     3121 ggtgatccgg cgcggaggcc ccgcaggcgg ggccgttgta aaacacggca tggccgggcg
     3181 ctgcgtcatg ggcgatatcc agaagcgcat ccagcgatga ccggatgtcc tgcggcagat
     3241 gtgatagcgc cgccacggtg aagtggcgct cgaagatggg cgcggggttg cacaagattt
     3301 gataatcatc gcggtaaaga attccggact gccgtgccgg gcgattttcg gcgcagagga
     3361 aacagggccg cttccggatc gattccgcat cgaccgccgc gccgctgctg atagcgcggg
     3421 cggggttata ctgcagggtg acgctgtagt cgccgcagga aagtttccgg gtttgcgccg
     3481 acgccaggcc gcggcaggcc gtagcatagg cgggccagct tttcttttgc gactcaacaa
     3541 gcgcgcggca gagcgcaggc agggttttgc ttctctctcc gggaaattca gcgaaatggc
     3601 gattcgaagt aagcgaagca gaggaaaccc tctgccggtc ggatatttga ggggatgccg
     3661 ccaatgcttt ctttgtcatt ccgggcaagc gaagcgcgac cgggaatcca gcattgaaag
     3721 attcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaatgac gaaactcttt
     3841 ttgattttta tcctctatct tccgggcggc aatttgattc gcgttcgcag gggatggttg
     3901 cttgtcagga acggtcttgc cgctcccaga cgtagtttga aaccgttccc tacggttttg
     3961 ttgttgccgg gcttttattt ccagcgttcg cagccggtcc ttgtaaaaat catttcggtt
     4021 ttgtttgtcc acggaaagct ccgcgtcggt gttgtccttc cagcggcggc aaagataaat
     4081 gctttgatac acccggccga tgcggtattc gcgggagagt ttcagcgcca cggcatagtc
     4141 ctcgccgtag ctcacattgg gaaagccgat ccgacgcaaa acagcggtgt caaaagcccg
     4201 cggcgcgccg atgccgccca cgcgcagaag attgttgtgg ccgtttcgca cggtccattc
     4261 ccggtgatcg atgagccccg gcggaatctt tttcccgcgc tcgttcacca gcgtatagga
     4321 gcccacggcc attgcgcaag gcccccggcg cagggcgtca accatggttt ggagtgtgcc
     4381 gggcgattcg taaagatcgt cggaatcgag ttgaacggcg tagcggccgc agtgaggaga
     4441 aaaaatcgcc tcgttccagc atccgccgat gcccagatcg cggcggaacg gcacaagatg
     4501 atgaacatgg ggataacgcg cggcaaagtc tttcaaaagc tccgtcgtgc cgtccgtgga
     4561 gaagttgtcc accgccagaa tattgaaggg aaagtccgtt ttttgcgcca gcgcgctttt
     4621 gatcgcgtcg gcaatggttt tccggcgatt gcgtacgggg atgacgatgc tggccttcca
     4681 gagaatctct tccggctgcc ggttcgcggc gggtcttgaa ggcagccggg cgcccaagcg
     4741 ccgcaaatgg ctggtggcga ttttttccag ctttttttgc cggaggaggt tttccccggc
     4801 aacgtaggaa aaatgcgctt cggattttcc gccgggtttt tttgacggca ccctttcttt
     4861 tgcggatacc gtgtataagg attcgggcac gcggatcaga tcatgctcca gcgaaattct
     4921 caggcgcaaa tcgtaaaaag ccatgtccgg atcgcccggc agagcgccgt attgttcaat
     4981 tgcggacaag gcagctctga ccgataaaat gaaaaaggga ccgaaatgaa aatcgtcgcg
     5041 cagggagccg ggctggtagt cgttgaccgg gtgtccggca aagcggccgt tgattttttc
     5101 gacgtagtcc gaatagacca gtcctgcccc cgtgtcgcgg gcgacggcca gcagtctcct
     5161 gagctccggg ggaccgcaaa gcacatcttt ttgcgcgtcc atcgccagca gataatccgc
     5221 cggcaggttt ttgaagaacg ttgacggccg cttcaccggc cggaaaggcc cgctgccgga
     5281 aggccggcgc agcgccgcct gtatttcatc cggggcgctc cagagcaaaa gccgtcccca
     5341 ttgatacact gaagttttca tgattttccg ggcgccgtca gaagtcggac atcatcagat
     5401 taaaggtatt ttccgccgtg tccttcgcca gcttggccag ggcctgctgt cgggccggtt
     5461 tcggattgac gttgtcctgc agcttgtaat ccgatgttcc gacaacgttc tgcgaagacc
     5521 agatggtttt gccgctgtcc ttttcgcgca agctggcctc cagggtgata gacattcttt
     5581 cttccgcggc cagaatgttg ttgcggtagg agagaactcc cgtggtgatg ctgaggatat
     5641 tgacgttgat gatggcgtcc gcctgctcga gagtgggcgc gcttttgaag cgattgctct
     5701 ggatgatctg gtcgatgaag gcgttgcgca aaatattttc cgcttccgct tccgcggttt
     5761 tgttgacaaa aggcgcgacg taaatattga cgatccgttt gtcgatatat tcgccctgcg
     5821 gcgcgaagcc gtagccgcaa ctgagcagca aaacgcagga caggctcaaa agcgacagtt
     5881 tgacagcgga caatcctctt ggttttttca tggatccgtt tcctttaggg agcgacgaca
     5941 atgttgacca gctttttggg aacgtaaatc actttcagaa ttttattttt cccgatgaac
     6001 gcggcgattt tttcatccgc ctgcgcgtcg gctttgatct tttccgcgtc gcaatcgacc
     6061 ggcacggtca tccggctgcg cagcttcccg ttgatctgga tgacgatcgt catttcttcc
     6121 tcgctggcga cggcgggatc gaagtcgggc cagggcatgt cggccgcggg cttgtccttg
     6181 cccagcctct cccagagctc ttccgtgatg tgcggaacga tcggcgtcag cagcagaatc
     6241 gccgtttcca gggcttcgcg gacgacggac agggccgtcc ggttgtcttt ggtcggatgc
     6301 ttgacgctgt ataagacatt gaccagttcc atgaccgcgc tgatggccgt gttgaagtgg
     6361 aagcggtctt cgatatcctg cgagaccttg cggatcgtct gatgcgtttt tcgccggagc
     6421 gccttgacgt cgtccgtcag ttccagcgga ccggcggccg gcgcggcccc ccggatgtct
     6481 tcgacatatt cgtcaaagat ccgccacagg cggccgagaa agcggaagga tccttccacg
     6541 ccctgttcgc tccattccag atctttttcc ggcggcgagg cgaacaggca gaagatgcgc
     6601 gcggtatcgg cgccgtaggt tttcaccaga aaatccgggt cgatgacgtt cttgagcgat
     6661 ttggacattt tttccgtctt gccgatgatg acgtctttgc cgcagaactg acacttgccg
     6721 ctttgggcct gttcggggaa gaggtagccg tgttcggggc acttcatcgt ttccttgcag
     6781 accatgccct gcgtcaggag attggtgaag ggctcatcga cgccgatcac gccgaagtcg
     6841 cgcagcacct tggtgtagaa ccgcgcgtag agcagatgca gaatggcgtg ctcgatgccg
     6901 ccgatgtact gatccaccgg catccagtag tcgagcgcct tgcggtcgag tcccggtttg
     6961 gtgttgtatc cggggcagca gaagcggtcg aagtaccagg atgattcgac gaacgtgtcc
     7021 atcgtatcgg tttcgcgcgc ggcggggccg ccgcattgcg ggcaggtggt gttcacgaag
     7081 tccggcaaag ccgcaagcgg cgagccgcct tcgggactga aaatgacgtt ttcgggcaga
     7141 atgaccggca gatctttttc cggcaccggc acgatgccgc attttttgca gtcgatcatc
     7201 gggatgggcg cgccccagta gcgctggcgg gagatgcccc agtcgcgcag gcggtactgg
     7261 atggtgcgct tgcccttgcc ttcggcttcc agataatcgg cgatcgcttc cagcactttt
     7321 gtgttttcca tgccgttgaa tgggccggag ttgaccagaa tcccttcatc aacgtaggcc
     7381 tcggtcatcg tcgccgcatc gagcgtccgg tctttgggag aaatgacgac gaccagctcc
     7441 agattgaatt ttttggcgaa ttcgaagtcg cgctggtcgt gcgtgggcac ggccatgacg
     7501 cagccggtgc cgtagtcggc caaaacgaag ttcgcggcgt aaatcggcat cttgtagccg
     7561 gtgagcggat tgaggcagta ggaatccaga aagaggcctt ccttctcgta atactcggag
     7621 gtccgcatga gcttgtcctg ctttttgact ttttccacaa acgcccgcac gtttttttcg
     7681 cagggcttcc ccttggccag ttcggtgacc agcggatgct cggccgccac cagcatgaag
     7741 gttgcgccga aaagcgtgtc ctggcgcgtg gtgaaaacct gaatgacgcc attgccttcc
     7801 gccattggaa aatcgaccag gcagccgtag cttttgccga tccagttttt ctgcatcgtc
     7861 atgacgcgct caggccatcc ggggagacgg tcgcagtatt ccagaagctc ctcggtgtag
     7921 gccgtgattt tgaagaacca ctgatcgagt gttttttccg taacttccgt gccgcagcgc
     7981 cagcacaggc cgccttcgac ctgttcgttg gcgagcaccg tgtcacacct ggcgcagaaa
     8041 ttcacgctgg agcgcttctt gtaagccagg cctttttcga ccatccagat gaaaaagagc
     8101 tgttcccatc ggtagtattg gggttcgcag gtggccactt cgcggtccca gtcgtagctg
     8161 aagcccatcc gcttgagctg ctttttcatg tggtcgatgt tttcatgcgt ccacctggac
     8221 ggatgaatct tgtgctcgat ggcggcattt tccgcgggca tgccgaaggc gtcccagccg
     8281 atcggatgga gaacgttgaa tcctttcata cgcttgtagc gggccaccac gtcgccgatg
     8341 gtgtaattgc gcacgtggcc gatatggatt ttccccgacg gatagggaaa catttccagc
     8401 aggtagtatt tctttttatt cggatcttcg acgactttga aggttttttc ttcttcccat
     8461 ttcttttgcc atttttcttc gatggcgatc ggatcgtact tcataacggt ggttttctcc
     8521 caaaagagtt caataatcgg ggatttgctt aacatattgg atggggcagc gcaacggctt
     8581 tgtcgggcgt cgaagtcggc tttctcctgc gggaaactaa ggctcctgct cttcggaccg
     8641 tctgacggtg atgtaaagga tcatgcctgc cagcatgacc gcggctgctc cgaagaaaag
     8701 agtggtgtag ccgaaagaat cgatcagccc tccggcgatg gccggaccca gaagcagcgt
     8761 cccgttctgg caggcctgat aaaggccgac gaatcccgct gtctggtctt tcgggatcat
     8821 gctgagaaag aaagggtagg agacggcaag caccgttcca taggcggcgc cggcgatgat
     8881 caccagaaaa agcagcgatt cgtaggtttg gaggaagggg atcacgcaaa aacagacgat
     8941 cagcgcggcg actccgccga tcgtggtttg tttcaatccc agcttcgcgc ccagcaaacc
     9001 gccgggcagg gcgaagccga caaaagaagc attgagcacg aagaatgctt taaccgcctg
     9061 ctcaacggac aggctgaaaa gcgccatgca ggtaatgacg aaaaaagcgc tcaccatcca
     9121 caaactgatc cattgcagcg tcatgccgga aaggaaacgc agcagatttt tccggccgga
     9181 aagctcccgg aacaggcgca ggcgcgtttt gccttgcggc ggaacggatt ccttcacgga
     9241 aaggcacatc acggcgacgg cggccggcac cgcaatcagc acccacacaa acgggcgggt
     9301 aggcgagact tcccaccacc gcgcggcaaa cagcaggtag ctcaggatgc cggttccccc
     9361 gatcagaaac atgacgcccg acgcgaagct tcgctgcgcc gcgggaatga tgtcgggcag
     9421 cagcgcgaaa taggggccga tggcgaagaa gaatccggca tacgccagga agatgacggg
     9481 gatcgcggcg gccagggagt gaatttgcgt cagcagaaga accatgctcc aggccagaag
     9541 ccatccggcg acgatcaggg gtcgacgccg ccccagcggc gtgcggatcc ggtcggacag
     9601 cgcgccggcc agtacgggaa agaacaggcc ggtgatgccg gtggtggaca aaatgacgcc
     9661 ggttcccgcg ttggaccccg tgagttcttt caggtagagc ggcagcgttg cccaggtgaa
     9721 tccccagaag gtctggaccg cgaaaaggcc catggcgaat cccgcgagtt tcaaaagccc
     9781 ggcgggtgaa ggttcggtgt gctccgggat cattcttttt cctgcaagat agacgcctcc
     9841 gtgctatccg gacggtcggc cgatcaggct gaaagtttgt ttgcgcaggt ccgggccagt
     9901 tcctttaaaa ccggcatcgt gcccgtctgg gacgcggcgg cggccaggtt ccgaatgccg
     9961 cccgcggtat gccggagaaa gtccggcttg tttttcttga gtcccagaaa accataggcg
    10021 cccagcgcct gcatcagccg ctgggccgcg ccttcccaga aatagtcggt gaacttttca
    10081 aagctgtaat ccggctccat gatccggtaa taaaaagcga caagttcatt gcgttcggac
    10141 gaagagaatg agacgtaggg gtcgcagatc agcgagccca ggtcgtaaaa aagattgccc
    10201 gttcgcatgc cctgaaagtc gagaaatacc ggccggtcat ttcgaatcat gatattctgg
    10261 gactgaaaat cccggtggat caggcattcc gggatttttt gcaggcggtc gatcagcgcc
    10321 tccatctctt ccgcccagtc gcgctcctgc gggccggaaa gcgaaagacc gcagacggcc
    10381 tcgacgaagt tttccctgaa gtaggcgtgt tcccaggcgt agagcgaccg gtcatacccc
    10441 cggcacagct ttaaagaagc cggcacatcg gatagcggag tccggtgaag cctgtggatt
    10501 ccggaaagag ccgtcagata gtggcgcctc cgttttcgcc agggagcggc gcgcagggaa
    10561 tacagatccg catcgcccag atcctcgagc agcatgaacc gctttcggaa gtcgaaggcg
    10621 atgatgcggg gaaccggcat gccgatctcg gtcatgaatc ggttgatctc cgcccagtag
    10681 gcgttttcct ccacgtcggt tccgtattcc ataaagatga aggtggagcc tccgggcacg
    10741 cgcacgcggt aaaaggtgcg gtcggagccg cccttgagga tcggatcgta ctgaaactcc
    10801 accgcgccca gatgatgatt tacaaattgt cgaatatccc ggttcataag gtctgatcgc
    10861 gtaatgtagt gtattcttcc atcgtgccca gatcgtgcca gaagccttca tcgatgacga
    10921 ttccgccgac ggaccgggga ttttgccgga tgctctcgac gagcggcagt acaatcgatt
    10981 cgattttccc cgccgaaagc cgccggagga aggccgtttc cagaacataa atgcccgcga
    11041 aaaggcagga ctggacgccc gggttgtgca aagcgtccct catatcgcag ataaatcccc
    11101 gagagtcaat acggacattc gtcagcggcc cccggctgcg cagcgccagc gtcgcctccg
    11161 tacaaagctc cgcgtgcctt ttcaggagag gttcaagcgg aaggttggtg ataatatcgc
    11221 cgttgtaaac gatgatgcgg cggtcctgtc cgatcagatc ctcgatattc ttgatgccgc
    11281 ccgccgtgtc cagaagcacg ggttcatggc ggaaaatgat gggaatgttc ccccatcggc
    11341 tctccgggaa cgcttcccgg tatttctccg ggcagtgatg cgtgttgatg ataaaacgct
    11401 ccacgcccgc ggcgcgcaga tgctccatcg cgtaagtgat gatcgggcgc ccgccgaccg
    11461 gcagcagcgg tttgggcaag tgttgcgtga gcggccgaag tctggtgccc agccccgcgc
    11521 ccagaatgaa agccgtgcgg atttttggct ctgccgaatc ggaaggacag cggttcatgg
    11581 ccgacaattc agcaggactt cgcgcaaaag tcaagcttga cggtcttgca agaagtcttt
    11641 ttcgccctct tttcgtctta ccgacgaagg ccggtagtaa ttacaatgag ttatgaacca
    11701 cggccttcgc cggggtgacg gattggtgtg ctttttacga gttcatcaag cttcgcgaat
    11761 gcgtttgccg gaccggagtt ttatgatata gaaaagcccg cagcttgcgg cttgacgtaa
    11821 agaaacaggg cgtggagacg cgggatgacg aacaagggcc tgcccggtca aaaagaaaga
    11881 gcagggagag ccgctgtcgg tttcccgggg ggcgattccc ggagagtaac cggtgaaggg
    11941 gcatttcgtt cgaccccgga aggattgttg acggatgcgg aagattatgc gacattccca
    12001 ccggcgcctt tcaacggcga aaggaaaaaa ataaaaatca gacaggatgc ctctatgaaa
    12061 aaaagatctg ttgccgtgtt gctgattgtc ctggtcctcg gcgcgtatcc ggcgcgggcc
    12121 atgaacgggc atgatcttgc cgatggaatg aaatcctatt taaaagccga gcaggactat
    12181 gccgcagcgg attacctgct ggcgggaaac tacatgggat atattcaggg agtggcggaa
    12241 gccacggttt ccgactactc tttgcccagc ggcgtcaccc ccgacacact gtataaaatc
    12301 gttgcggatt acctgcagaa aaaccccgaa aaatgggaca aaccggcgtc gttcctggtg
    12361 cgcagcgccc tgattgccgc ctatcccaaa tatctgatcc gaaataaata gcgcaaagga
    12421 gctgttgagt gaaaaaattg atgctggccg ggatgctgct gctgttgttt tcaggatgtt
    12481 cgcccgcaca aaagatatcg ccggaagaac ggtttaaaaa aagctttccc cggcattcct
    12541 acgaaaccat tacgcaaacc tccgcaaaag gcgtctatga agtctataac ggcagccaga
    12601 tttattatta tcttccggat ggcgacatga ttttcgtggg cagcatgatt tccaaagacg
    12661 ggaccaatct taccgaggag agcaacggga aaaaaatgac ctccaaaatg gcggggctgt
    12721 ccctggacaa ggccctgaaa atcggcgaag ggaaaacctc cgttgtggaa ttcatcgacc
    12781 cgaactgcca ttattgccgt cagtcccacg cctattttgc cggacgaatg aaggaggtca
    12841 ccctgtatgt ctttttctat cctttgtcca gggattcgga ggacaaaatc cggcatatta
    12901 tttgcgccgc cgacaaatcc ggagaatacg ccaaggtgtt gtcgggagag ctggacgaaa
    12961 agaagccgct gaatctttgc ggcgacaagg atgccgaggc tctgatcgcg gcgcaccggg
    13021 aggcctccgc gaaaatcggc gtgcgcgcga cgcctttgtt ttatatcaag gggcgggtcg
    13081 tgcccggttt tgacaggccg gtgattgaag ctctgttaaa aaagtgaagg ggaagctctt
    13141 tttcatctca ccttggcgcc ggttttgggc tcgcggtcga agccgatcag cgtcaatcct
    13201 tccggagcat ccgtcgccag caacatgccg tgcgactcga cgcccatcag tttggccggc
    13261 ttcaggttgg ccacgatgac aatggatttg ccgaccaggt cttccggctt gtagtctttg
    13321 ccgatgcccg ccacaatcgt ccgctcttct ccgatgtcaa ttcgcagctt gaccagcttg
    13381 ttggatttgg ggactgcctc cgccgcgaga atcttcgcca cgcgcagatc cactttggaa
    13441 aattcgtcgt agtcgatctc cggtttgagt tcgacggccg gctttttcgg cggcggcgct
    13501 tccttttcgg cgctcacgcg cgggaagagc gattcgccgc gggaaagcgc cgcacccgcc
    13561 ggcaggggtt cgccccggcg gatcgtgtcg agattgaatt tggctgtctc ctccagtccg
    13621 agcgagccca aaattttttc ggacgcctgc ggcatgaacg gcgcaatcaa gatggcgacg
    13681 gaacgcaaaa cctccagcag ccggtacatg atggcaagga gcttttcgcg gtttgccggg
    13741 tctttggcca gattccaggg ttcgttttcc acgatgtgct tattggcggc gccaaccagc
    13801 tcccagatgg ccatgagcgc cttgtgcagc gacaggtcgg caaagcaggc ttccacttcc
    13861 gccacggtct tttgcgccag ctccgggagc ctgacttcca gcgccgcgtc gggcggcgcg
    13921 ggaatttttc cgtcgcagta tttgacggcc atcgtgaccg agcggctcac caggttgccc
    13981 aggtcgttgg ccagatcgga attgagcctc gcgacaaacg cctcttccga gaaattggaa
    14041 tcgaggccga acaccatgtc gcgcagcagg aagtaccgga aggcatccag gccgtacttg
    14101 tctttcagat ccagcggccg cacgacattg cccaggcttt tggacatttt gctcgagtcg
    14161 gaattccagt agccgtgtac gttgaggtgc cggtaggggt cgatgcccgc ggctttgagc
    14221 atggtgggcc agtagattcc gtgcggcttt aaaatgtcct tcgcgatcag gtgctgggcc
    14281 tcgggccaga attttttgaa gttcggtccg tccggataac cgatggctgt cacgtagttg
    14341 atgagcgcgt caaaccagac atacgtgaca tactggtcgt cgaacggcag cgtaatgccc
    14401 cattccaggc gtgtcttcgg ccgcgagatg cacagatctt ccagcggttc gcgcagaaag
    14461 gccaggactt cgttgcggta gcgttccggc cggatgaaat cggggttttc ctgaatgtgc
    14521 cggacgagcc agtcccggta tttgctcatg cggaaaaagt aattgctttc cttgcggtgc
    14581 tccggttcgg tctggtgatc ggggcatttg ccttccacca gctctttttc cgtgtaaaac
    14641 cgctcgcagc cgacgcaata gaagccttcg taggagccga agtagatgtc gcccgcgtcg
    14701 taaaccttct gcaggatgcg ccggacggtt tccgtgtgat tttcatcggt cgtgcggatg
    14761 aaataatcgt tggtgatggc cagctcgggc cagagggcgc gaaactgcgc gctgacgccg
    14821 tccgcgtagg ctttcggggt gatccccgct ttttgcgcgg cctcggcgat tttgtcgccg
    14881 tgttcgtccg tgccggtcac aaaaaacgtt tcgcgtccgg tcatgcgggc gaagcgcgcc
    14941 aggacgtcgg cgacgatggt tgtataggcg tggccgatat gcggcgaggc gttgacgtaa
    15001 taaatcggcg tggtgatgta aaagggtctg gacatggtgg ttggctgctc cttataccgc
    15061 tttccgtttc tttttgtttt ccctggagcc atttggcctc tgccggatct gatgcggcgg
    15121 ttcactaccc tccgggactt cccggacggg ttcgattcgg gccgcttccc gggcgctctt
    15181 tcgggcgtcg gcgtatccct cgtgttcaaa ggacaggcag cacatcagcc ttccgcacaa
    15241 gccggaaatt ttttccggat tgagcgacat gctttgttgt ttggccatct tgacggttac
    15301 gcggtccagg ttctgcaaaa tgcccgcgca gcagaccgtc cggccgcaaa ccgcgattcc
    15361 cttgacgatt ctcgcctcct ggcgcgctcc gatctgcttg agctcaatcc gcgttttgaa
    15421 tctggaaacc aggtccttga ccagttcccg gaaatccacc cgggcgtccg ccgtaaaata
    15481 gaaaatcatt ttgcttcggt cgaaaaggca ttccaccgtg atcaatttca tggaaaggcc
    15541 cttttcggcg attttgccga cgcagtattt tttggcttcc tcttccagca gttccagctc
    15601 ttcgcggacc cgcaaatcgt ccgcggtcac cttgcggatg acgtttttca gattgggggg
    15661 cggatcggag gcgtcgcagt ggcggacgtc ggtgacgacc gttcccagcg cgacgccgtt
    15721 gtccgtattg accagcacct gatcgttttt gtgcaggatc aggtcggccg cgtcgaaatg
    15781 atagatgcgg ccttcttttt tgaattttac gccaaggatg ttgatctgca tgcgccctgc
    15841 tttgcctttt ttacagatga agcttaaatg ccattgcgtc caatgtcaac gatttgttgg
    15901 cgttcatgtc cagcgattgc ctggattttt ccacgacggc aatattgtgc agcagatcgt
    15961 cgccttccag acggcccgcc agcgactcga cgaccggcag gtcgtccgcg ttgaggagca
    16021 tggacgcccg ggccgtttcc ctgtaaacca gcgcgtcgcg gaaatatgta tggagaatgg
    16081 acaggccctg ctgcgtgtgt tttttatcct ggccgagaaa agacgcgagg acgatcaggc
    16141 tcatcggatc gcggcgtccg gcatcccgga gcaggccgct caatccggcg cgaaaggcga
    16201 tcatgtcctc ggcatccagc tccagcgccc ggccgatcga gccgccggaa agggaggcga
    16261 gcgccgaggc gtgggcgggc tccatgcgct tttgctccgt caaaaaccgc gcgacgtctc
    16321 cggcggcgag cgggttgaag cgcaaatggc ggcagcggga gagaatcgtt tgcggaagcc
    16381 ggaagggacg ggccgtcacc aggatgagaa tattgtcggc ggacggctcc tccagggttt
    16441 tcaaaagggc gttggccgcg ggttcgttca ttttgtccgc ctcatcgatg atgaagacgc
    16501 gctttcctcc ttccagcggc cggaaggtca tctggtcctg aatgccgcgg acgtcgtcga
    16561 tgcggataaa ctgtgtctgc gcctggagga catgaatgtc cgggtggctg ccgtgtgtcg
    16621 ttttccggca ggaggggcat tggccgcagg catcctgccg gagggcggcg ttttcacagt
    16681 tctgcgcctg cgcgaattcc agcgccagcg tttttttgcc gatggctgaa agtccgctga
    16741 aaatgtaaga atgcccgacc cgcttttggg ccatggcttt ctgcagcatg gaaatttgtt
    16801 tcgtatggcc gtaaatattt gcaaatgaca tcggaataaa accgcctggc acatcagcgc
    16861 tgtttgtgga tgaaatccat tacctgtccg cggacctgat ctgcgatttc ctccacgctt
    16921 ccggatgcgt cgatcaatcg aaaccgctcg ggttccgccg tgtacaaatt cagataagcc
    16981 tgccgcaccc ggttgtggaa gtcgagtttc tcccgctcga agcggtcggc ggccgcggga
    17041 ttggtcagcc gggcgtcgcg ccggccggca cgctcaagtc cggtttccac cggcagatca
    17101 aaaagaagcg tcagcgacgg tcttacctgc atcgcgcaat agtcgttggc cgcggcgacg
    17161 aaagcgggat ccagcccccg gcccgccgcc tggtaggcaa aggtcgcgtc ggaataccgg
    17221 tcgcacagca cggtttttcc gctttggagc gcgggaagga ccacctcccg cacatgctgt
    17281 gcgcgggccg ccaggaaaag cagaagctcc gtttccgggc acatggcaaa atggccgcgg
    17341 tggaaaagga tatcgccgat tttacgaccg atcggcgttc ccgaaggctc ctgtgtcagg
    17401 accacgggaa ttttttcccc ggtcaggtaa tcgcccagaa gttggatttg cgtggatttg
    17461 ccggagcctt cgactccttc aaaagtgatg aatctgttca tgaacggttc tcccggcgtc
    17521 atggggtgag agctttgaat aacggccatt tttgtcattt cgaccgaagg gagaaatctt
//
LOCUS       DAOSRM010000042        16968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3487_length_16968_cov_122.174777, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000042 DAOSRM010000000
VERSION     DAOSRM010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 16968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16968
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3487_length_16968_cov_122.174777"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..3603)
                     /gene="smc"
                     /locus_tag="PLA58_05705"
     CDS             complement(55..3603)
                     /gene="smc"
                     /locus_tag="PLA58_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOR62325.1"
                     /translation="MKLKRLEITGFKSFREKVLIDFSKGVNAVVGPNGCGKSNVVDAI
                     RWVMGEQRVKALRGKKMDDVIFNGSEDAAPVSMAEVVMTLSSDGSSFPGVYGELTEVS
                     ISRKTILDGESEYCINRVPCRLLDVKEFFMGTGVGARTYSLVEQGAVANLVEAKPEDR
                     RLFIEDAAGVSKYKSRKDAAVRKMEATKQNILRLNDIHKEVKTQLNAITRQAKKAEQY
                     KAVKQRMKETELALSRQGYAKMAGKNAALQDERTRISGQADAVGAQMESRESALQEMK
                     AALLEKDEQIGLRQTELYEVKNSINIKEKSVEFSRRRMAETSERKQKNLAEIEEMGRK
                     KESLAAEIDNLQRKSEDLLRQMTLLQQELEEGEREVQGLLEADRDVNARLEEQKIRYI
                     DVMQDLAKFGNAVAGLKKGMEDFQRREEREIREIEQDKSRLADLTQKMQELAGGLAEE
                     EEQIAQVGKEKEAAADALEKAKSDLQAVEERILEVKEEAGAKSSRLQSLAEFEAAFKW
                     SNEGVKKVIETQDSPDAFYGVVADHIDVPREYESAVEAVLGDKLQYVVVKNQEEGVRA
                     IDYLKSYKLGRGSFVPVELRGGSAQTYSEEHLQEAEPLLRKVVVQEEFKKIADCLLGD
                     VLLTPSIETGINLWKKNGFRGTFVTPEGDTISPNGVLTGGSGSAVEKSLLATKREIAE
                     LEREVRKLSGELEDRMEEKKRLASSIARWDEELARLKNSEHQLEMNAHSRKKDLERFG
                     DESERLKQRIAVLEFNRRNIKTEEAQARRKLQELEAEILVKEAAEKELNEIISGFNSR
                     RQQSRAAIDAQEKRLTEKKVLLAALAEKKEADLRTIARVQNDLAASESEAARKREDIA
                     ACEQQLAGLTAAVGADTEALKILYEKLASCEAVLSELREKRTGDDERLKVQENEIRDI
                     KRKMDELRRQQSELEMQCREGALNLENLQNAVAEKHQVDLASLMADFEMMEDEKIAEL
                     GALLEKDKQTIENFGEVNLLALNEYAELDQRNQFLTTQLSDLNESLASLQRTITRINK
                     ISRERFSETFAAVNECFKKTFAHIFPGGRGELLLTDESDLLETGVDIDIQIPGKKAQS
                     VSLFSGGEKSLAAIALIFAILLYRPSPFLILDEVDAALDDANTNLFNRLIRDVAEKSQ
                     VIMITHNKSTMEVADTLFGVTMQKQGISSLVSVTLN"
     gene            complement(4097..5107)
                     /gene="amrS"
                     /locus_tag="PLA58_05710"
     CDS             complement(4097..5107)
                     /gene="amrS"
                     /locus_tag="PLA58_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOR62326.1"
                     /translation="MIREALLYEKLAGSRVLCGLCAHRCNIGPNRRGLCGVRENREGV
                     LYSLVFGTLIAEHVDPIEKKPFFHVHPGSRSYSVATAGCNFTCAFCQNHEISQMPRST
                     LMIMGEEAPPAMIVEGARKNGCQTIAYTYTEPTVYLETALETAKIACREGLKNLFVTN
                     GFMTPQAIDLMSPYLAAANVDLKSFRDEFYKKQCGARLDPVLDSLRKMKDEGVWLEIT
                     TLLIPGLNDSTEELKDIAAFIRDLGPETPWHISRFHPQFKMLDTPSTPIASLRRACAI
                     GKEAGLYHVYSGNVPGEEGENTYCSNCHKLLIRRYGFRIIDYSLSGHQCSFCGQKLEG
                     LL"
     gene            complement(5104..5886)
                     /locus_tag="PLA58_05715"
     CDS             complement(5104..5886)
                     /locus_tag="PLA58_05715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR62327.1"
                     /translation="MIFDPLQAVTGDDWLQTRQGRRLYLRQEKLILDLVAPVAGESVL
                     DVGCGSGNTLRMFGRKKCLLTGMDPSAAALESARRNLGASCELVLGNAADIPFSDNEF
                     DVVTLTGGLQGSANPQKALAEAVRVSRNRVFVGFLNRYSSAGTEQAVRNFFGFPAASN
                     LRFFSIGEMQTLIHKTMSGAQIQWGSVMCLPGPAVALLSSLEDLFPMRNNPLGAFVGM
                     VFSVNYTYRTVQHPVLNSFKLKARERAAAPEAVRDMLREGKP"
     gene            complement(5912..6904)
                     /gene="moaA"
                     /locus_tag="PLA58_05720"
     CDS             complement(5912..6904)
                     /gene="moaA"
                     /locus_tag="PLA58_05720"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOR62328.1"
                     /translation="MLDAYNRDINYLRISITDRCNLRCIYCRPMEGISLKGHDDILRY
                     EEILRVVSRAVTLGFVKVRLTGGEPLVRRGFVEFAADLKKTKGLEDISLTTNGILLAK
                     YAEAIFNAGITRLNISLDSMDRDKYAQITRGGNLDDVFRGIAAAEQAGFSPIKINTVI
                     VKGFNDNEVLDFAQLALVKPFQVRFIEIMPVSPVSDGYTADFLPNRDLQETIGRHFRL
                     TPLAGKQNKSDGPARIFTLPDGLGEIGFIDPVSDHFCATCNRLRLMADGKLRACLLKD
                     EEIDLKAALRQNCSDGELDELIRRAVRLKPEKHDLDCTDRHLKKCHRDMSDIGG"
     gene            7213..7683
                     /gene="greA"
                     /locus_tag="PLA58_05725"
     CDS             7213..7683
                     /gene="greA"
                     /locus_tag="PLA58_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOR62329.1"
                     /translation="MDKVPITKEGFDKLKKELETVKNVFIPENIRDIEVARAHGDLSE
                     NAEYAAAKERQSYLHGKLQELENNLASCNIIALKDIPSDRVVFGCFVTIADSEGEEIK
                     YQLVGPFESDINQNKISVTSPIGRALVGKSIDAEISVNTPGGTRNFQIIDISAE"
     gene            complement(7758..8174)
                     /locus_tag="PLA58_05730"
     CDS             complement(7758..8174)
                     /locus_tag="PLA58_05730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOR62330.1"
                     /translation="MSDELILEVVTPEKMAFSGKVDEVTIPGTEGEFGVLRGHEPFLT
                     SVDIGELYYIADGKKTYYAVNTGYAEVTGSKVTVLIETAERADAVDKDRALKAKEKAE
                     AALSQLTKEHSDYEKMRLALLRAVNRISVAEKLSQN"
     gene            complement(8190..9596)
                     /gene="atpD"
                     /locus_tag="PLA58_05735"
     CDS             complement(8190..9596)
                     /gene="atpD"
                     /locus_tag="PLA58_05735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOR62331.1"
                     /translation="MNIGKIVQVIGPVVDVAFEEGKLPSILDALLVTNPAINDQEDNL
                     VIEVAQHLGDNVVRCIAMDVTDGLVRGMKAKDSGAPITVPVGKEGLGRILNVVGRPVD
                     GLGPVNAKNHAPIHRKSPEFTEQDTSVHVLETGVKVIDLLVPFPRGGKMGMFGGAGVG
                     KTVVMMEMINNIAMHHGGISVFCGVGERTREGNDLYREMLESGVIKQAALIYGQMTEP
                     PGARARVALTGLAAAEYFRDVEGQDVLFFVDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLASDLGELQERITSTTKGSITAVQCVYVPADDLTDPAPATTFAHLDGTVVLSRPIAE
                     LGIYPAVDPLDSTSRILDPNVLGQEHYQVARQVQVTLQKYKDLQDIIAILGMDELSEA
                     DKMTVSRARKIQRFLSQPFFVAAQFTGTEGKFVSVADTVRGFKEILEGKHDDLPEQAF
                     YMVGGIEEAIEKAQKLSA"
     gene            complement(9658..10536)
                     /gene="atpG"
                     /locus_tag="PLA58_05740"
     CDS             complement(9658..10536)
                     /gene="atpG"
                     /locus_tag="PLA58_05740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOR62332.1"
                     /translation="MPSLKDIKRKVTAVQKTKQITRAMNMVAASKFKSSQLKMENFRP
                     YAGKFMDVLNSLALRVDTSTHPLLAVRDPRRIRVICMTSDRGLCGGFNTNLIKATERF
                     LRDKTHEGKEVAMINVGRKGRDFFRKKANVLAQKVDCLSKFDMTLAVSIADEVIEPFI
                     KAEYDELYLIYNQFVNVSVQRPTVVRLFPLPSIGSPDADLESDTRLDYVYEPSDEVLL
                     HKLLPMYVHVLVYRALLETSAGENGARMAAMDNATSNCEELIRTLTLKMNKARQAAIT
                     AELMDIVGGTEALAKG"
     gene            complement(10613..12127)
                     /gene="atpA"
                     /locus_tag="PLA58_05745"
     CDS             complement(10613..12127)
                     /gene="atpA"
                     /locus_tag="PLA58_05745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOR62333.1"
                     /translation="METIKAEEISQIISEQIKSYEKKLDISETGTVLSVGDGIARVYG
                     VQNAMAMELLEFPGGILGMVLNLEADNVGVAVLGEVTHIKEGDIVKRTGKIAQVPVGE
                     GLLGRVIDATGAPLDGKGPIESTEFRRIEMIAPGVIQRQPVNQPMYTGLKAIDAMTPI
                     GRGQRELVIGDRQIGKTAICVDAIIRQKDTGIKCIYVAIGQKKSTVAQVVERLKQHDA
                     LSYTCVVAGCASDPATLQYIAAYAGCSIGEYFRDRGQDALIIYDDLSKQAVAYRQISL
                     LLRRPPGREAYPGDIFYNHSRLLERAARVSKELGGGSLTALPIIETQAGDVSAYIPTN
                     VISITDGQVYLEPSLFFSGIRPAINVGLSVSRVGGAAQVKAMKQVAGTLKLDLAQYRE
                     LAAFAQFGSDLDKSTQAQLDRGVRLVELLKQPQFQPMSLGEEVVVLFAGSRGFIDKVD
                     VEKVLIYEQQLLSYVKSKHQDILKEIDEKQIISADLEKKMKDMLTAFESVFVAE"
     gene            complement(12131..12673)
                     /gene="atpH"
                     /locus_tag="PLA58_05750"
     CDS             complement(12131..12673)
                     /gene="atpH"
                     /locus_tag="PLA58_05750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOR62334.1"
                     /translation="MISNISKRYARAFFDIAGEEQKLEQYYNELHQFSSVIAQNKDLG
                     GFLANPIFEQEIKKKVLEKIIGKLSLSPMTINFLKLLIDKKRIDILPDIETCYRQLMD
                     ETLQKVRVTVKTAFPLSGEMQSYITSSLKKMTGRTVDVTVENDKNLLGGIVIGVGDTL
                     YDGSIKNQLNNMRNLLGEAR"
     gene            complement(12670..13263)
                     /locus_tag="PLA58_05755"
     CDS             complement(12670..13263)
                     /locus_tag="PLA58_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOR62335.1"
                     /translation="MKSSFRSVHFSILLSLVLLLLSVGLVFASGGEGGEGHGSQWKDF
                     AWKTFNAVLIIGFLVYLLGARIKGFFSGRRQEIKESLESATVQKAEAEKQYREYAEKI
                     DKASQEIDGIFEMIKAQGVVEKQQIIEDAHKVAQKMKEDAQARIEQELKKASGQLRAE
                     AVALSVQMAEEILKKNITAQDHEVMVREYMDKVVNKN"
     gene            complement(13260..13694)
                     /locus_tag="PLA58_05760"
     CDS             complement(13260..13694)
                     /locus_tag="PLA58_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOR62336.1"
                     /translation="MVTVIPDYTLLVQMATFIALIFILNFLLYKPLLSIIERRKKQLD
                     ELGNEIKLFNESVNKKAAEYEEKLSRAKTSASDLKKQIIGEGAAEAKRIVDAVRSEIP
                     LMTQEFQKKMDAEMQAARQILEGQSRRLSLEIAEKVLGRRVQ"
     gene            complement(13985..14362)
                     /locus_tag="PLA58_05765"
     CDS             complement(13985..14362)
                     /locus_tag="PLA58_05765"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOR62337.1"
                     /translation="MWEKKKRLDDTKAFLGKGAEFIGKLIFTGHVRIDGDFQGEIFGQ
                     GAVDIGEGALVKANMEVGSVFIGGEVQGNIDVKEKINIHSTGKLAGDVKAPVFIMEEG
                     AVFDGQSRMSEVKEQKRIALAGK"
     gene            complement(14394..15509)
                     /gene="rodA"
                     /locus_tag="PLA58_05770"
     CDS             complement(14394..15509)
                     /gene="rodA"
                     /locus_tag="PLA58_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOR62338.1"
                     /translation="MFFFRYDRRIFQNFDWTLFALVMSICAIGVVNIYSTGFSLGENA
                     SPLYLKQMQWVFFGLFFMMVAFLIDYRYINRAAYGLYVFSVASLIAVALFGHTANGAQ
                     RWISFGVFLFQPSELMKISIILALARYFDDHKSREPYRLRELLIPFGMVMIPCALILK
                     QPDLGTALMIVIVSASIVLFIGMNWKSVAIAVASIFVMLPLAWHFLKDYQKNRLLTFL
                     SPENDPLGTGYHIIQSMIAIGSGGFLGKGFLKGYQTQLKFLPEQQTDFVFSVFAEEWG
                     FLGGLALMVIFTSLIIWGLKIALHSKDLLGTIIAFGITALIAWQVLINMGMVLGVLPV
                     VGIPLPFISYGGSAVVSLMAAIGLLMNVSVRRFILQR"
     gene            complement(15547..>16968)
                     /gene="mrdA"
                     /locus_tag="PLA58_05775"
     CDS             complement(15547..>16968)
                     /gene="mrdA"
                     /locus_tag="PLA58_05775"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOR62339.1"
                     /translation="VDVTPIRLYLDGEITAPIIGYTGEVSKEELEKPEGDYASGDITG
                     KSGIEKALDPYVRGRRGNELVEVNVYGKVIKNLGRIDPVCGSNVVLTIDAALQKAAWE
                     AFEGRPGAAVAMDPRDGSILAMVSSPSYDPNLFNSGIAREAWEKLQKDPLKPMSNRAI
                     AGQYPPGSTYKLIVAAAALEEGVITPETRINCSGSFDLGNRTYRCWRKNGHGPVNLHR
                     ALVESCDVYFYTVGKKLGVDRIARYARKFGLGDVTGIELAHERKGLVPTRDWKQARMK
                     EPWQMGETISISIGQGFNLTTPLQLAQAYASLANGGTLWRPRLVQRIEAAEGGVVKEY
                     LPEKKGDLGLSGKTVELLQQALWGVVNEPGGTGYAARIDGADVCGKTGTSQVVGLPQD
                     EQARRRKKIAAFHKDHALFACYAPARHPEIVVSVVAEHAGGGGAVAAPIARRILKAYF
                     DGKKKDRPVEVAVRRQTAGQAGN"
BASE COUNT         3593 a         4959 c         4393 g         4023 t
ORIGIN      
        1 acgcccttgc ctgcggcggg ggaatccgcg ccgaaagcga tagaacgggc cggatcaatt
       61 gagggtgacg gataccaggg aggaaatccc ctgcttttgc atggtcacgc cgaagagcgt
      121 gtcggcgact tccatcgtgc tcttgttgtg cgtaatcatg atgacctggg atttttcggc
      181 gacatcccgg atcaggcggt tgaagaggtt ggtgtttgcg tcgtcgagcg ccgcatcgac
      241 ttcatccaga atcaggaagg gcgacggacg gtacagcagg atggcgaaga tcagcgcgat
      301 cgccgccagg gatttttcac cgccggagaa aaggcttacg ctttgcgctt tcttgcccgg
      361 aatctgaatg tcgatatcca cgccggtttc cagaagatcg ctttcatcgg tcaggagaag
      421 ttcgccccgc ccgccgggga aaatgtgggc gaaggttttc ttgaagcatt cgttgacggc
      481 ggcaaaggtt tcggaaaacc gttcgcggga gattttattg atccgggtga tggtcctctg
      541 cagcgacgcc agcgactcgt tgaggtcgct caactgcgtc gtcagaaact ggttgcgctg
      601 atccagctcg gcatattcgt tgagcgccag gagattcact tcgccgaaat tttcgatcgt
      661 ctgcttgtcc ttttccagca gggcgccgag ttcggcaatt ttttcatcct ccatcatctc
      721 aaaatccgcc atgagagagg caagatcgac ctggtgcttt tccgcaaccg cgttttgcag
      781 gttttcaagg ttcagcgcgc cctcgcggca ctgcatttcc agctcgcttt gctgccggcg
      841 cagctcgtcc atttttctct tgatgtcgcg gatttcattt tcctgaactt tgagccgctc
      901 gtcgtccccg gtccgcttct ccctcagttc gctcagaacc gcttcacagg acgccagttt
      961 ttcgtaaagg atcttgagcg cctccgtgtc cgccccgacg gcggcggtaa gaccggccaa
     1021 ctgctgttcg caggccgcga tgtcctcccg ctttcgcgcg gcttcgcttt cgcttgcggc
     1081 gagatcattc tgcacccgcg cgatcgtccg caaatccgct tccttctttt ccgccagcgc
     1141 ggccagcaga acttttttct ccgtgagccg cttttcctgg gcgtcaatgg ccgcgcgcga
     1201 ctgctggcgg cggctgttga agccggagat gatttcgttg agttcctttt ccgccgcctc
     1261 cttcaccagg atttccgcct cgagctcctg cagtttccgc cgggcctggg cctcctccgt
     1321 cttgatgttc cggcggttga actccagaac ggcgattctc tgcttgagcc tttccgattc
     1381 atcgccgaag cgctccagat ccttcttccg gctgtgggcg ttcatttcca gttgatgttc
     1441 gctgtttttc agcctggcaa gctcttcgtc ccaacgggca atcgacgagg ccagtcgctt
     1501 cttttcctcc atccggtcct cgagttcgcc ggaaagcttc cggacttcac gctccagctc
     1561 cgcgatttcc cgcttggtgg cgagcaggct tttttccacc gccgatccgc tgccgccggt
     1621 caggacgccg ttggggctga tggtgtctcc ctcgggtgtc acgaaggtcc cccggaagcc
     1681 gtttttcttc cagagattaa tgccggtttc aatggacggc gtcagcagga catcgcccag
     1741 caggcagtcg gcaatctttt tgaattcctc ctggacgacg actttgcgca ggaggggctc
     1801 cgcttcctga agatgctcct cgctgtaagt ctgggcgctc ccgcctcgca gctccaccgg
     1861 cacgaaactg ccccggccca gcttgtaact tttcagataa tcgatggcgc ggacgccttc
     1921 ttcctggttc ttcacaacga catactgcag cttgtcgccc agcaccgctt ccacggccga
     1981 ttcgtattcg cggggaacgt cgatgtggtc ggccacgacg ccgtaaaacg cgtccgggct
     2041 gtcctgcgtt tcaatgactt ttttcacgcc ctcgttggac catttgaagg ctgcctcaaa
     2101 ttccgcaagc gattgcagcc gcgatgattt ggcgcccgct tcttccttga cctcgaggat
     2161 gcgctcctcg acggcctgca ggtccgactt ggctttttcc agcgcatcgg cggccgcttc
     2221 tttctctttg ccgacctgcg cgatttgctc ttcttcctcc gccagcccgc cggccagctc
     2281 ctgcatcttc tgcgtcaaat cggcaagccg gcttttgtcc tgttcgattt cccggatttc
     2341 cctctcttca cgccgctgga aatcctccat gcctttcttc agtcccgcga cggcgtttcc
     2401 gaatttggcc aggtcctgca tcacgtcgat atagcggatt ttttgctcct ccagacgggc
     2461 gttgacgtcc cggtcggctt ccagcaggcc ctgaacttca cgctcgcctt cttccagttc
     2521 ctgctgcaaa agggtcatct gccgcaggag gtcttcggat ttcctttgca gattgtcgat
     2581 ctcggcggcg aggctctcct ttttccggcc catctcttca atttccgcca ggttcttctg
     2641 cttgcgctcc gaggtttccg ccattcggcg gcgagaaaac tcaacgctct tttccttgat
     2701 gttgatggaa ttcttcacct cgtaaagctc agtctggcga aggccgatct gctcgtcttt
     2761 ttccaggagg gccgccttca tctcctgcag ggcggattcc ctggattcca tctgcgcccc
     2821 gaccgcgtcc gcctgcccgg agattcgcgt ccgctcgtcc tgcagcgcgg cgtttttccc
     2881 ggccattttg gcatagccct gacgggacaa cgccagttcc gtttctttca ttcgctgctt
     2941 tacggctttg tactgctctg cttttttggc ctggcgggta atggcgttga gttgggtttt
     3001 gacttccttg tgaatgtcgt tgagtcgcag gatgttctgt ttggtggcct ccatcttgcg
     3061 aacggcggca tccttgcggc ttttatactt ggacacgccg gctgcatctt cgatgaaaag
     3121 ccggcggtct tccggtttgg cttccaccag attggccaca gccccctgct ccaccaggga
     3181 ataggtccgg gcgccgacac ccgtgcccat gaagaactct ttcacgtcca gcaggcggca
     3241 gggcacgcgg ttgatgcagt actcgctctc gccgtcgagg atggttttgc gcgaaatgga
     3301 cacttccgtc agttcgccgt aaacgcccgg gaaggacgat ccgtcgctcg acagcgtcat
     3361 cacgacttcc gccatgctca caggggcggc atcctcactg ccgttgaaaa tcacgtcgtc
     3421 catcttcttg ccgcgcaggg ctttgacccg ctgttcgccc atcacccagc gaatcgcatc
     3481 gacaacattt gattttccgc agccgttggg cccgacaacc gcattgacgc ccttggaaaa
     3541 atcaatcaaa accttctcgc ggaaagattt gaaacctgtt atttcaagac gctttagctt
     3601 cataaatcat ctttaattga aatcgttgta aattttggca aagcctaaca aaagaatgtt
     3661 tctttgtaaa gggaaaatac aacaaaatgt attcccgaaa aaacactacc acaacatata
     3721 gtgcttaccc gttcatttcc gggggccggt ttttacagaa aaaccgtctt gaaaacagga
     3781 aatcggcagg aaaaaagaat cacggtttaa cggccgatgt gatgaatctg gtctttggcg
     3841 agcccgttcc gttgctcaaa tatcactttg agcggccatt aagggcgttt tgtcacataa
     3901 atcttggcga agcgaaagat cagcaatgac aactgtttga gccgaaggcg agttttgtca
     3961 ttgcctttcg cgagccttag atttatacaa aacgctctca ggcaagcgaa aagaatgttt
     4021 gggcaacact ctgtgtctca gtccgccaca gagtgaatgc aattgattca ctcccgcaaa
     4081 cgatatctct caacatttaa agcaggcctt ccagcttctg accgcagaaa gaacactggt
     4141 gtccagaaag ggaataatca ataattctaa atccatagcg tctgatcaac aatttatggc
     4201 agtttgaaca gtaggtgttt tcaccctcct cgcccggcac attgccgctg taaacatgat
     4261 acagtccggc ttctttcccg atcgcacagg cgcgtcgcag ggaggcgatg ggagtggaag
     4321 gggtatcgag cattttgaac tgcggatgga acctgctgat atgccaaggg gtttccggac
     4381 ccaagtctcg gatgaaagcg gcaatatcct ttaattcttc ggtggaatcg ttcaatccgg
     4441 gaattaaaag cgtggtaatt tccagccaga caccctcgtc tttcattttt ctgaggctgt
     4501 ccaaaacggg atcaagtctc gcgccgcatt gctttttata aaattcgtcc cgaaaagact
     4561 tcaaatccac attggcggca gccagataag gcgacatcag atcaatggcc tgaggcgtca
     4621 tgaatccgtt cgtgacgaaa aggtttttca gaccttcccg gcaggcgatc ttcgcggttt
     4681 ccagagcggt ttccagataa accgtcggct ccgtgtacgt ataggcgatc gtttggcaac
     4741 catttttcct ggccccttcg acaatcatcg ccggcggcgc ttcttccccc atgatcatga
     4801 gcgtggagcg gggcatctgt gaaatttcat ggttctggca gaaggcgcag gtaaaattgc
     4861 agccggcggt ggccacggag taagaccgcg aaccgggatg cacatgaaaa aagggcttct
     4921 tttcaatcgg atccacatgc tcggcaatca gcgttccgaa aacaagggag tacaaaacac
     4981 cttcccggtt ttcccgaacg ccgcacagac cgcgccggtt gggcccaatg ttgcagcggt
     5041 gggcgcatag cccgcagagc acccgagacc cggccagctt ctcgtaaagc agcgcctcgc
     5101 ggatcatggt tttccctccc gcagcatgtc ccgaacggct tccggagcgg cggctctctc
     5161 tcttgctttc agcttgaaag aatttaaaac gggatgctga acggttcgat acgtatagtt
     5221 gacggaaaaa accatcccga caaacgcccc gagtggattg ttcctcatcg gaaaaaggtc
     5281 ttccagggac gacaacagag caaccgccgg tccgggaaga cacatcacgg acccccattg
     5341 aatttgagcg ccggacatgg ttttgtgaat cagggtctgc atttctccga tggaaaagaa
     5401 acgtaaattt gatgcggcag gaaaaccgaa aaaattccgc acggcctgtt cggttccggc
     5461 cgaagaatac cggttcagaa aaccgacaaa aacacgattg cggctgaccc ggaccgcttc
     5521 cgcaagcgct ttttgcggat ttgctgagcc ctgcaggccg cccgtcaacg taaccacatc
     5581 gaactcattg tcggaaaaag gaatatcggc ggcattgccc aaaaccagtt cgcacgatgc
     5641 gccaagattt ctccgggccg actccagggc agccgctgac ggatccattc ccgtgagcag
     5701 gcattttttc ctgccgaaca ttcgcagagt gtttccgctt ccacaaccca cgtccagaac
     5761 ggattccccg gcgacgggag cgaccagatc caggatcagt ttttcctgcc gcaaatacag
     5821 gcgacggccc tggcgggtct gaagccagtc atcccctgtc acggcctgaa gcggatcaaa
     5881 aatcatggat cgccactcct tttgaaaaag gtcagccccc gatgtccgac atgtcccggt
     5941 ggcatttttt caggtgtctg tccgtgcagt ccaggtcgtg cttttcgggt ttgagccgaa
     6001 cggcccggcg gatcagctcg tccagttcgc cgtcgctgca gttttggcga agcgccgctt
     6061 ttaaatcgat ttcctcatcc ttgagcaggc aggcgcgcag tttgccgtcc gccatcaggc
     6121 gcaggcggtt gcaggtcgcg cagaaatggt cgctgaccgg gtcgataaac ccgatttctc
     6181 ccagcccgtc cggcagtgta aagattctgg ccgggccgtc cgacttgttt tgcttgcccg
     6241 ccaggggcgt aagccggaaa tgacggccga tggtttcctg cagatcacgg ttcggcaaaa
     6301 aatccgccgt gtatccgtcg ctgaccgggc tgaccggcat gatttcgatg aaacgaacct
     6361 gaaaaggttt gacaagcgcc aattgcgcaa aatccagcac ctcgttgtcg ttgaatccct
     6421 tgacgatcac ggtattgatt ttgatggggg agaagcccgc ttgctccgcc gcggcaatcc
     6481 cgcgaaacac atcgtccagg ttcccgcccc gcgtgatttg cgcgtacttg tctctgtcca
     6541 tggaatccag actgatgttg agccgggtga tgccggcgtt gaaaatcgcc tccgcgtatt
     6601 tcgcaagcag gatgccgttg gttgtcagac tgatgtcctc gaggcctttt gtttttttca
     6661 aatccgcggc aaattcaaca aaaccgcggc ggaccagcgg ctcgccgccg gtcagccgca
     6721 ccttgacaaa tcccagggtc accgcccggg aaaccacgcg gagaatctcc tcgtaccgga
     6781 gaatatcgtc atgacctttc agcgaaatcc cttccatggg gcggcagtat atgcagcgca
     6841 gattacagcg gtcggtgatg gaaatccgaa gataattgat gtcccggttg tatgcatcca
     6901 gcatgagatc aaaccaatgc tcctttaatt ttctgaccgt cgttcaataa cacaaaccgc
     6961 ttcacaaaac aacttccgcc tcgcccggac gacttcatga agcggcgttt tcccgccgga
     7021 gaaacatccg gcgcggacgc aaacgagcgg ccccggatgc attttgagta cgggcaaccc
     7081 ttcgcctcaa atatcccaaa caatttcttg acacggcgtt ctttctcgtc taaacaaaaa
     7141 tcaaacgcgt ttcctgcctg tcctcgcacc atcgattgcg tcagcgcaag gtaaaatcat
     7201 tcggagccgt tcatggacaa agtgccgatc acgaaagagg gttttgacaa attgaagaag
     7261 gaactggaga cggtgaaaaa cgttttcatt ccggaaaaca tccgggacat agaagttgcc
     7321 cgggcgcacg gcgatctttc ggaaaacgcg gagtacgcgg cggccaaaga gcggcaatcc
     7381 tatctgcacg gcaaacttca ggaactggaa aataatctgg cttcctgcaa tatcatcgcc
     7441 ctgaaagaca tcccgagcga ccgggtggtt ttcggctgct ttgttaccat cgcggacagc
     7501 gagggggaag agatcaaata ccagctggtc ggcccttttg aatcggacat caaccaaaat
     7561 aaaatatccg tcacctcgcc catcggcagg gcgctggttg gaaaaagcat cgacgccgaa
     7621 atttccgtca acaccccggg gggaacccgc aattttcaga tcatcgacat ttcggccgaa
     7681 tagccaattc cctctgaaaa ataaaaaaag gcggctccgg tcgggagagc tgcctttttt
     7741 ccctctatgg agacagatca gttttgcgac agtttttccg caacactgat ccggttgacc
     7801 gcccgaagaa gcgccagtct cattttttca tagtcgctgt gctctttggt caattggctc
     7861 agagcggctt ccgctttttc ttttgccttg agagcccggt ccttgtccac ggcgtccgct
     7921 ctttccgccg tttcgatcag gacggtgacc ttgctgcccg tcacctccgc gtagccggtg
     7981 ttgacggcgt aataggtttt cttgccgtcg gcgatgtaat aaagctcgcc gatgtccaca
     8041 gacgtcagga agggctcatg gcctcttaaa acgccgaatt ccccttccgt gcccggaatc
     8101 gtcacttcat ccactttccc ggaaaaagcc atcttctccg gcgttaccac ttctagaatc
     8161 aattcatccg acatggaaat ccccgttcat taggcggaaa gcttctgggc tttctcgatt
     8221 gcttcttcaa tgccgccgac catataaaag gcctgttccg gcagatcgtc atgtttgcct
     8281 tccagaattt ctttgaagcc tctcacggta tcggccacgg agacaaactt tccttccgtt
     8341 ccggtgaact gtgcggcgac gaagaagggc tgcgacagga atctctgaat ctttctggcg
     8401 cggctgacgg tcatcttgtc ggcttcggaa agttcatcca taccaagaat ggcaatgatg
     8461 tcctgcaggt ctttatactt ctgcagcgtc acctgcacct ggcgggccac ctggtaatgc
     8521 tcctgcccca aaacattcgg gtccagaatt ctggatgtgg aatccagcgg atccacggcg
     8581 gggtaaatgc cgagttcggc gatggggcgg gacaaaacga cggttccgtc aaggtgcgca
     8641 aacgtggttg cgggagcggg gtcggtcagg tcgtcggcgg gcacgtacac gcactgcacc
     8701 gccgtaatgg agcctttggt ggtcgaggtg atgcgttcct gaagctcccc gaggtccgaa
     8761 gccagggtcg gctggtaacc gacggcggac ggcatgcggc ccaggagtgc ggacacttcg
     8821 gaaccggcct gcgtgaaacg gaaaatgttg tcaacgaaga agagcacgtc ctggccttcc
     8881 acatcgcgga agtattccgc ggccgccagt ccggtcaacg ccacgcgggc tcgggctccg
     8941 ggcggttcgg tcatctggcc gtaaatcaga gcggcctgct tgataacgcc ggattccagc
     9001 atttcacggt acagatcgtt tccttcgcgg gttctctcgc caacgccgca gaacacggaa
     9061 ataccgccgt gatgcatggc gatgttgttg atcatttcca tcatgacgac ggttttgccg
     9121 acgcctgcgc cgccgaacat gcccatcttt ccgccgcggg gaaacggcac gagcagatcg
     9181 ataaccttga cgccggtttc cagaacgtga acggacgtgt cctgttccgt aaattcggga
     9241 gatttgcgat gaatgggagc atgattttta gcgttcaccg gtccgaggcc atccacgggg
     9301 cggccgacca cgttcagaat tctgccgagg ccttctttgc ccacggggac ggtgatcgga
     9361 gcgccggaat ccttggcttt catccctcgc accagaccgt cggtgacatc catggcgatg
     9421 cagcgaacaa cattgtcgcc cagatgctgc gcgacttcaa taaccaggtt atcctcctgg
     9481 tcgttgatcg cggggttggt aaccaaaagc gcatccaaaa tgctgggaag cttcccttct
     9541 tcaaaagcca cgtcaacaac aggaccaata acctgaacaa tctttccgat attcataact
     9601 atctccttgc aaatagtcgg tatatatatt ttttacgcgc aaaatgccgc cgcaaaatca
     9661 acccttggcc aaagcctccg tgccgccaac gatatccatc agttccgccg taatcgccgc
     9721 ctgcctcgcc ttgttcattt tcaacgtcag cgtgcggatc agctcttcgc agttgctggt
     9781 cgcgttgtcc atcgccgcca tccgcgcgcc gttttcaccg gcggaggtct ccagcagcgc
     9841 ccggtaaacc agaacgtgga catacatggg aagcagtttg tgtaagagca cctcgtccga
     9901 cggttcgtaa acataatcca gccgagtatc cgattccaga tcggcgtccg ggctgccgat
     9961 ggaaggaagg gggaacaggc ggacaacggt cggccgctgg acggaaacat ttacaaactg
    10021 attgtagatc agatacagct cgtcatattc ggccttgatg aaaggttcaa tgacttcatc
    10081 cgcaatcgac acggcgagcg tcatatcaaa cttgctcaga cagtccactt tttgcgccag
    10141 aacgtttgct tttttgcgga aaaagtccct gcccttcctg ccgacgttga tcatggcaac
    10201 ttctttgcct tcgtgagtct tgtcccgtaa aaatcgctcc gtggccttga tcaggtttgt
    10261 attgaatcca ccgcaaagcc cccggtcgga cgtcatgcaa atcacccgga ttctcctggg
    10321 atcgcgcacg gccagaagcg gatgggtgga ggtgtccacc cgcagagcca gactgttgag
    10381 aacatccatg aactttccgg cgtagggccg gaaattttcc attttcaact gagatgactt
    10441 aaatttggat gcggccacca tgttcatggc ccgcgtaatc tgcttcgttt tctgaacggc
    10501 agttactttt cttttaatat cttttagcga gggcactgga aactcctctc aacctgaatt
    10561 gttttccact ccgcggcgat cctcttggca aggatcgcgc atggatcaaa aattattccg
    10621 ccacaaaaac agattcaaaa gccgtcagca tatctttcat tttcttttcc aggtcggcgg
    10681 aaataatctg cttctcatcg atttctttca ggatgtcctg atgcttgctt ttcacataac
    10741 tgagcagctg ctgttcgtaa atgagaacct tttccacgtc caccttatcg atgaagccgc
    10801 ggcttccggc aaacagaacc accacttcct ctcccagaga catcggctgg aactggggct
    10861 gtttcagcaa ttcgaccaga cgaacaccgc ggtccagctg ggcctgggtt gatttgtcca
    10921 gatcggagcc gaactgggca aaggccgcca gttcgcggta ctgggccaga tcgagcttca
    10981 gggtgccggc gacctgtttc atcgccttca cctgggccgc gccgccgacg cgggacacgg
    11041 acagaccgac gttgatggcc ggacggatgc cggaaaagaa caaactgggt tccaggtaca
    11101 cctggccgtc ggtgatggaa atcacgttgg tcggaatgta ggccgatacg tcgccggcct
    11161 gcgtttcaat gatgggcaga gccgtgagcg atccgccgcc cagctccttg ctcacgcggg
    11221 cggcccgctc gagcagacgg gagtggttgt agaaaatgtc gccggggtaa gcttcgcgtc
    11281 cgggcggacg gcgaagcagc aacgaaatct gacgataggc cacggcctgt ttggacaggt
    11341 cgtcgtaaat aatgagggcg tcctggccgc ggtcacggaa gtattcgccg atgctgcagc
    11401 cggcgtaagc cgcgatgtac tgaagggttg ccgggtcgga ggcgcagccg gcgaccacac
    11461 aggtatagga cagggcgtca tgctgtttga gcctttccac aacctgcgcc acggtggatt
    11521 ttttctggcc gatcgcgacg tagatgcatt tgatgccggt gtctttctgg cggatgatgg
    11581 catccacgca gatggcggtt ttgccgatct ggcggtcgcc gatgaccagt tcgcgctggc
    11641 cgcgtccgat cggggtcatg gcatcgatgg ctttgaggcc cgtgtacatg ggctggttga
    11701 ccggctggcg ctgaatcacg ccgggggcga tcatttcaat acggcggaac tccgttgatt
    11761 cgatcggtcc tttgccgtcc aggggagcgc ctgtcgcgtc gatgacgcgg cccagaagcc
    11821 cctctccgac cggaacctgg gcaatctttc cggttctttt gacgatatcc ccttccttaa
    11881 tatgggtcac ttctcccaga accgcgacgc cgacgttgtc cgcttcgagg ttgagcacca
    11941 tgcccagaat accgccgggg aactcaagaa gttccatggc catagcgttt tgcacgccgt
    12001 acactctcgc gatgccgtca ccgacggaaa ggacggttcc ggtttcgctt atatccagct
    12061 ttttttcata gcttttgatc tgctcagaaa taatttgact gatttcttcc gccttgatgg
    12121 tttccatgct ctatcttgcc tcccctaaga gattcctcat attgttcaat tggtttttga
    12181 tgctgccgtc atacaacgta tcaccaacac caataactat gccgcccaac agattcttgt
    12241 cgttctccac cgtcacgtca acggtccggc ccgtcatttt tttcaggctg gacgtaatgt
    12301 agctctgcat ttccccggac aacggaaacg ccgtcttcac cgtaactctc actttctgaa
    12361 gcgtttcatc catcaactgc cggtagcagg tctcgatgtc cgggaggatg tcgatccttt
    12421 tcttgtctat cagcaatttt aaaaaattaa tcgtcatggg tgaaagcgac agcttgccga
    12481 tgattttctc cagcactttt ttcttgattt cctgttcaaa gatcggattg gccagaaaac
    12541 ctcccagatc cttgttttgc gcgatgacgg aagagaactg atgcagttcg ttgtagtact
    12601 gctccagctt ctgttcttct ccggcaatgt caaaaaaagc gcgggcgtat cgttttgata
    12661 tattgctgat caatttttgt tcaccacctt atccatatat tccctgacca tgacttcatg
    12721 atcctgagct gtgatgttct tctttaaaat ttcttccgcc atttgcacgg aaagggcgac
    12781 cgcttccgcg cgaagctgcc cggaggcttt tttcaattcc tgctcaatgc gcgcctgggc
    12841 gtcctccttc atcttctggg ccaccttgtg cgcgtcctcg atgatttgct gcttttcgac
    12901 cactccctgc gccttgatca tctcaaagat gccgtcaatc tcctgggagg ccttgtcgat
    12961 tttctcggca tactcccggt actgtttttc ggcttccgcc ttttgaacgg ttgcgctctc
    13021 cagagattcc ttgatttcct ggcggcggcc ggaaaagaaa cctttgattc tcgccccaag
    13081 caaatacacg aggaacccga tgatcagaac ggcattgaag gttttccagg caaagtcttt
    13141 ccactgcgag ccgtgcccct cgccgccttc gccgccggac gcaaaaacca gccccacgga
    13201 aagcagcagc aaaaccaggg agagaagaat tgaaaaatgg accgaacgaa atgaagattt
    13261 cattgaactc tccttcccag aaccttttcg gcaatttcca gcgagagccg gcgggactga
    13321 ccttcaagga tctgacgggc ggcctgcatt tccgcgtcca tcttcttttg aaactcctgg
    13381 gtcatgaggg gaatctcact gcgaacggcg tccacgattc ttttcgcctc cgcggcgcct
    13441 tcgccgatga tttgcttttt caggtccgaa gcgctggtct tggcccggct cagcttttcc
    13501 tcgtattcgg cggccttttt attgacggac tcattgaaaa gcttgatctc gttgcccagt
    13561 tcatccagct gcttcttgcg gcgctcgatg atggacaaaa ggggtttgta gaggagaaaa
    13621 ttgaggatga aaatcaaggc tatgaacgta gccatctgaa ccaggagcgt ataatcggga
    13681 attacggtaa ccacaactga cctcgtcttt ttttcagacc ttcccggcca tcaacgaacg
    13741 ttgatcaacg caggaagatg cattaaagtt gatccgacat ttcaaacagc gtaatctctg
    13801 ggagtgattt gcttcagata acgtaaggct tgaatcaagc cgggcttatg ctgaacctag
    13861 ccacaccgcg aggacggagg ttactaatca cacccgccta gggtttgtca agctttttta
    13921 tgaccggcgg tctaatttcg caaaaatcgg atttaagcta ataaaattta tcacttggga
    13981 attattattt tccagccaaa gcgattcttt tttgctcttt cacctcgctc atccgcgatt
    14041 ggccgtcaaa aaccgctccc tcctccataa tgaaaaccgg cgccttcaca tcgcctgcaa
    14101 gtttccccgt ggaatgaatg ttgatttttt ccttcacgtc gatattgccc tgaacttcgc
    14161 cgccgatgaa aacgctgccg acttccatgt tggcctttac cagggcgcct tctccgatat
    14221 ccaccgctcc ctgtccgaaa atttccccct gaaaatcgcc gtcaatgcgc acatggccgg
    14281 taaaaatcag cttcccgata aattcggcgc ccttccccaa aaaagctttg gtgtcgtcaa
    14341 gacgtttctt tttttcccac atgccgcact ccttagtcgt cctgaaaata aaatcagcgc
    14401 tgcaggatga atctgcgcac gctcacattc atcagcagtc cgatcgccgc catcagagaa
    14461 acaaccgccg agccgccgta actgatgaaa ggcagcggaa tgccgaccac cggcaggacg
    14521 cccagcacca ttcccatatt aatcagcacc tgccaggcga tcagcgcggt aatgccgaac
    14581 gcgatgatcg tgcccaataa atcttttgaa tgcaaggcaa tttttaaccc ccagatgatc
    14641 agggatgtaa aaatcaccat cagcgccagg ccgcccagaa acccccactc ctcggcaaac
    14701 acggaaaaca caaaatcggt ctgctgttcc ggcagaaact tgagctgggt ctgatagccc
    14761 ttcaggaagc ccttgcccag aaatcccccg gagccgattg cgatcatgga ctgaatgatg
    14821 tgataccccg tgcccagcgg gtcattctcg ggggaaagaa acgtcagcag gcggtttttc
    14881 tgataatcct ttaaaaaatg ccaggccagg ggaagcatga cgaaaatact cgcgacggca
    14941 atcgcgacgg atttccagtt cattccgata aaaagaacaa tggatgcgga aacaatgacg
    15001 atcatcagcg ccgtccccaa atccggctgc ttcaaaatca gggcgcaggg aatcatcacc
    15061 atcccgaacg gaatcagcaa ttccctcaac cggtaaggtt cgcgcgactt gtgatcatca
    15121 aaataccggg ccagcgcgag gatgatgctg atcttcatca gctcggaagg ctggaataaa
    15181 aaaacgccga acgagatcca ccgctgcgcc ccgttcgcgg tgtggccgaa cagagcgacc
    15241 gcgatcaggg aggccaccga aaaaacgtaa aggccgtacg ccgcgcgatt gatgtaacgg
    15301 tagtcgatca aaaaggcgac catcatgaaa aagaggccga agaaaaccca ttgcatctgc
    15361 tttaaataca gcggagacgc gttttcgccc aggctgaacc ccgtggaata gatattgacg
    15421 acgccaatgg cgcagatcga catcaccagg gcaaacagcg tccagtcaaa attctggaag
    15481 atgcggcgat catatctgaa aaaaaacata aactcctcct cgcctgtccc cgcggcctcg
    15541 gaaccttcag tttccggctt gcccggccgt ctgccgccgg acggccactt caacgggcct
    15601 gtcttttttc ttcccgtcga aataggcctt taaaatcctg cgggcaatcg gagcggcgac
    15661 cgctccgcct ccgccggcat gctcggcaac gacggaaacg acgatttccg gatgcctcgc
    15721 gggggcataa caggcaaaca acgcatgatc cttgtgaaag gcggcgattt ttttccggcg
    15781 gcgcgcctgt tcatcctggg gcagaccgac aacctgcgac gtccccgttt tgccgcagac
    15841 gtccgcgccg tcgatgcggg ccgcgtagcc ggtccccccg ggttcgttca cgacgcccca
    15901 cagcgcctgc tggagcagct ccacggtctt cccgctcaag cccagatctc ccttcttttc
    15961 cggcaaatat tctttcacca cgccgccttc ggccgcttca atccgctgaa ccagacgggg
    16021 gcgccacaac gtgccgccgt tggcgagcga cgcatacgcc tgcgccagct gcagcggagt
    16081 ggtcaaattg aatccctggc cgatggaaat cgatatggtc tcgcccatct gccacggttc
    16141 tttcatgcgg gcctgcttcc aatcccgcgt ggggaccaga cctttgcgct catgggccag
    16201 ctcaatgccg gtgacgtcgc ccagaccgaa ctttctggcg tagcgcgcga tgcgatccac
    16261 gcccagtttt ttcccgacgg tataaaaata gacatcgcac gactccacca gcgcccggtg
    16321 aagattgacg ggcccgtgcc cgttttttct ccagcagcga taggtccggt tgcccagatc
    16381 gaaagaaccg ctgcagttga tccgggtttc gggggtgatc accccctctt ccagcgccgc
    16441 ggccgccacg atcagcttat aggttgaccc cggcggatac tgccccgcaa tcgcccggtt
    16501 ggacatgggt ttcagcggat ccttctgcag cttttcccat gcttcacggg caatgccgct
    16561 gttgaataaa ttcgggtcgt aagacggcga gctgaccatc gccaggatgg agccgtcccg
    16621 ggggtccatc gcgaccgccg ctcccggcct tccttcaaag gcctcccagg ccgccttctg
    16681 gagggcggcg tcgatcgtca gcaccacatt ggaaccgcaa accggatcga tccgccccag
    16741 attcttgatg actttgccgt acacgttcac ttcgaccagc tcattgcccc ggcggccgcg
    16801 cacgtaagga tccagcgctt tttcaatgcc cgattttccc gtgatgtcgc cggaggcgta
    16861 atccccctcc ggcttttcca gctcttcctt gctgacttct cccgtgtagc cgatgatcgg
    16921 cgcggtgatt tccccatcca ggtaaagacg gatcggtgtg acatccac
//
LOCUS       DAOSRM010000043        16582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3584_length_16582_cov_102.487203, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000043 DAOSRM010000000
VERSION     DAOSRM010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 16582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16582
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3584_length_16582_cov_102.487203"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..1849)
                     /locus_tag="PLA58_05780"
     CDS             complement(278..1849)
                     /locus_tag="PLA58_05780"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOR62340.1"
                     /translation="MGDDIRQATTEGKLRDFDARVQALTQMGGAKQVQKQHDGGKLTA
                     RERLDRLFDEGSFQEVQLFVKHHATLFGMDKKEIPADGVITGFGKVNGRIVFAASQDF
                     TSAGGTLGEMHAKKIWKVMDMALDAQKPFISINDSGGARIQEGVPSLEGYGGIFYRNT
                     LASGYIPQITAIMGPTAGGAVYSPALTDWIFMVKNTSYMYITGPDVIKAVIGEEVSQE
                     DLGGAMAHASKSGVCHVVTENDEDCIRKIKELLSYLPDSCHSPLPVAVATDSPDRECP
                     ELNKIIPDRAARAYNMKNVIKSVADNSELFELHAQWAPNIIVALIRIMGSPVGVIANN
                     PMSFAGVLNVNASDKASRFIRFCDAFNIPLLTFSDVPGYMPGTDQEWAGIIRHGAKLL
                     HAYSEATVPKITVVTRKAYGGSYIGMCCKQLGADYVMAWPTAEIAVMGASGACNIIHR
                     AEIKAAADPEAKRAELIADYEEKFNNPYYAASLGAIEEVILPSQTRRRIVVLLEALRN
                     KKEARREKKHNNIPL"
     gene            2044..2766
                     /gene="truA"
                     /locus_tag="PLA58_05785"
     CDS             2044..2766
                     /gene="truA"
                     /locus_tag="PLA58_05785"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOR62341.1"
                     /translation="MILEYDGAAYCGWQRQLNGRSIQQVVEEALERIIGESVTVIASG
                     RTDAGVHAMNQAASFRSHTGLSANKIFLGVNSVLPDDIVVKDLQEAPPDFNALKDAKG
                     KVYLYRIRNQRLRPALGRQYVWHVRYPLNLDRMRQAASRLIGEHDFSCFCATGCDIKD
                     RVRLLTVLSIDTPENGLMEITLESSGFLRHMVRNIVGTLVDVGRGKIAPERIPEILAS
                     RDRKQAGTAAPACGLFLKEVLY"
     gene            2770..3645
                     /gene="rfbD"
                     /locus_tag="PLA58_05790"
     CDS             2770..3645
                     /gene="rfbD"
                     /locus_tag="PLA58_05790"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOR62342.1"
                     /translation="MKVLLLGHKGMLGNDLMAQFRHRHDVIGMDREEIDITRASECRR
                     AVAEIQPQLVINAAGYTNVDACETAKEECFAVNAEAVKNIAQACHDQNITIIHYSTDY
                     VFDGNADHPYTEEDPCNPINTYGASKLAGEHHLKRLTDNFILIRTAWLYGAKGKNFVG
                     AILDRARETGKLTVVDDQMGSPTCTRDLAAATELLVDKNARGIFHVTNRGSCTWYDFA
                     RKILKEARLDQVELSPMKTGELQRAARRPAYSVLGMQKFVAATGKTMQPWQLAFSDYY
                     SFVTSRTCMSPPGTA"
     gene            3830..6577
                     /locus_tag="PLA58_05795"
     CDS             3830..6577
                     /locus_tag="PLA58_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HOR62343.1"
                     /translation="MRNIFKIVFWGLIPAAALMLQGCLVSTPSTLISPEIRSLYEGEY
                     KIDPYMKKHVPLSTAVLPFVDLSGSKEGGNAMRRGFYNHFSSLPFKDMELYKVDDLLA
                     KAGLTDAEAVRKTSPQKLGEILGVDAVVFGEVSDFDKYFAVLYSQVAVGAEVKMYDTK
                     TGNFLWSGKHKVRKHEGGVSTNPVGIIATVIATALNVRDIQLLRANDDLFRDMVKTIP
                     TPSLAQAKRPPVISLLTQDTKGLPKKAGDEIKVVIQGTPNMQASFDIGQYRKRIEMQE
                     VEPGGYYGVYKVVPGDNIEKAIVTGYLTDDSGNTAHWVDAIGSVTLDTTPPDNPTGIT
                     SKGRNRLVALRWEKNTAADLAGYLIYRSDTPLSGYVQIGKTELNEYSDADPNLANSRT
                     YYYRISAVDFAGNESRMAQAQGMPIAPGPTPVSGIIEADTTWYSGASPYILESNVTVK
                     DKALLTIEPGTEIQSKGGALIVEGRISARGDNDSIIRFDSAEENQLWPGISFASVKDR
                     ENHLAFVRVRNARTAVLCQASSPTIEKSEFTQNQEAIRIQGAFSKPIISKNALYKNKG
                     VVISVSDGAAPRIVENTINDNDNTGLMLSSSAAEISQNTITRNKNNGIVVKGAPAVIS
                     QNNLVDNQPYNLFGEMKGAPAKALNNWWGSAKMAVVLSGIHGRVDVGSVLDGPYPQGK
                     ALAVPILDKKLEGAIQADAYLILSHSPYRVAKDVVIDGGATLYIEPGVVVEFEQGTSF
                     ITQNGGMVARGTREKPITFTAASSSPAPGFYVSAVRFSESTKVNSAFAYCIVKYATTA
                     FDVYTGSPEISYSYIAHASQSAVYCRKDAAPVLSYNTFAQNQGEGAVKCVGSANPRIF
                     QNNFIDNAVAVQSFSSIYIDARNNWWGSAPPDPKIFWGENINTKPWLEQANPEAFTER
                     R"
     gene            6632..8590
                     /locus_tag="PLA58_05800"
     CDS             6632..8590
                     /locus_tag="PLA58_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOR62344.1"
                     /translation="MIYFRNVSLSFLDRHLFDQISWTIHPKSRIGLVGDNGAGKTTLF
                     RAILDEVRLDCGLIEIPGRKQKKIGYLPQDLVELEATGLIEFLRRKSGIAQLEEDLRT
                     LERQISQIQSDTSEYGELSRRYAQATAEFSARDGYAFESRAKQILKGFGFRESDFDKT
                     CDVFSGGWKMRILLTSILLSQPDIMLLDEPTNHLDTESMEWLESYLKDYPGTILVVSH
                     DHFFLDKIATQIAELANRRIHLYSGNYTYYLAEKERRREALKKEMELQKAQIKKIEDF
                     VERFRYKATKASQVQSRVKMLEKFRDIQPEGSAKSVVMKFPEGKKSVKEVVAVSDLGH
                     AYGDLTVFSGLNFSLFRGEKVAFVGVNGSGKSTLSRLLSLSEKPSHGSVTYGDGVNMA
                     FFSQESSQNLHYANTIWEEVQAVPTSADDQTKRNLLGAFLFSGDDIFKPVGVLSGGEK
                     SRLALLKIMLLDTNFLVLDEPTNHLDIKTKDVFQNALLHYSGTVAIVSHDRYFLDRLV
                     NKVFELKDGEIRAYAGNYSYFIEKRAERTQDTGQQHRRDRAAVDRTLTAKDKKRLEAE
                     ERNRLSKIKNGLKRQLAEVEAKITDLESRKAANENSLCDPQTHKDAGKIKELQIDLKA
                     FEKELAQAYRLWTELSCKLEETDSAGSA"
     gene            complement(8692..9210)
                     /locus_tag="PLA58_05805"
     CDS             complement(8692..9210)
                     /locus_tag="PLA58_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOR62345.1"
                     /translation="MDKRMNALAFALENEEKEREFYLAQARKTRNMAGKNMFKQIADE
                     EKEHYEMLKKLHAKWVEKKKWPATIPLKVQPSSAGSVLQGMSGKKSARITGTEDELKA
                     VRTAIDFEARGVALYSKLAKQCTDAKEVAFFDMLAQVERQHLLSLKDTEEFLVDPATW
                     YQHMERSGLDGA"
     gene            complement(9295..10215)
                     /locus_tag="PLA58_05810"
     CDS             complement(9295..10215)
                     /locus_tag="PLA58_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOR62346.1"
                     /translation="MLDAIDKTLLKTVAELEELPKGAYNIRKNGKLLSRHVSANINIE
                     TNADETGIIVKIKPGTKNESVHIPVILSQAGLYDVVYNAFIVGDDAEVTIIAGCGIHC
                     GSSEPEGHAGIHEFKVGKNAKVTYVEKHYASGEGAGRRTLNPTTRVFLAEGAHAQMEL
                     TQIGGVDEANRINEAVLGPDSLLLITERVLTDGEQTAVSTNNIVLKGAGSRANMISRS
                     VMKGNSKQIFYATIDAQNKCYGHIECDAIIMDNGTNETMPSLKAGHPDAELTHEASIG
                     KIANDQLTKLMSLGLTYDQAVNRIIQGFLK"
     gene            complement(10196..10951)
                     /locus_tag="PLA58_05815"
     CDS             complement(10196..10951)
                     /locus_tag="PLA58_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR62347.1"
                     /translation="MLELNKVYFTVSDSEGSGGVRNIIDGIDFTFDDGKFYVITGPNG
                     SGKTTLAKLIMGINPLSSGDILFNGRNISTLSITERAKAGIAYSFQQPARFKGITFRD
                     LLTVATGIEDESKLLEILGRVGICPLSFLDKEVDARLSGGEIKKIELATTIAANPKLA
                     IYDEPDTGIDLWTIGPMVSLLKKELAENCTTTIVVSHNKAFLEAADVVMIIHSGKIVY
                     NGSLEGAMPLLDDLSVCTYRDSCEGDRDAGCYR"
     gene            11197..11400
                     /locus_tag="PLA58_05820"
     CDS             11197..11400
                     /locus_tag="PLA58_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62348.1"
                     /translation="MADQYYFLAAFFTTFFFAAGFFAATFFFAAGFFAAAFFTTFFFA
                     AGFFAAAFFTTFFAVAAIGFSLL"
     gene            complement(11637..12401)
                     /locus_tag="PLA58_05825"
     CDS             complement(11637..12401)
                     /locus_tag="PLA58_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOR62349.1"
                     /translation="MKKICFRLAVLLLMGLLMQACAGREAKVYPAAPKPQKKQLAQMG
                     HTIQAGAFARVENAVRLTEKLKGQGLPATYFRAADGLFKVRFGNFTTKESALLRAQSL
                     RQAGVIEEYYIVLPEDSAAAKRSQYGTDYLRESLVKTAHDFIGVPYLWGGISADSGFD
                     CSGLTMTVYQLNGLDLPRHSRTQYDAGDSIAREDLQKGDLVFFDTRGRGKVSHVGIYI
                     GAGRFIHAPSSGKKIRIDALSSDYYARRFVGAKTYL"
     gene            complement(12428..13777)
                     /locus_tag="PLA58_05830"
     CDS             complement(12428..13777)
                     /locus_tag="PLA58_05830"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOR62350.1"
                     /translation="MIRTADDHSIEKHRGAKRVVEGLRRAGFEAYFVGGCVRDYVRGV
                     SPGDYDIATSALPDQVIRLFERTVAVGAKFGVVVVREGGFSYEVATFRSDEGYEDGRR
                     PTQVRFSSAREDVLRRDFTINGLLMDPETNAIIDYVGGRADIGKKMIRTIGDPGVRFN
                     EDHLRMLRAIRFAANLDFHLDDGVRDAIARHAVKIREISAERVQEELNKILTRSGARR
                     GLEWMAETGLLKQVLPEVDRMRGVAQPPRFHPEGDVWRHTLIMMEMLSGEGKPEADAA
                     LAWGALLHDVGKPVTRTEDENGVHFYGHVKRGESIADDVMRRLRFSRTLREKVLDLIG
                     RHMAFMNVQKMRPARLKRFLRMPNFHLHLQLHRLDCLASHGMLDHYEFCREQLQKAEP
                     EELHPPRLLTGDDLLAMGYAPGKRMGEMLRALEEEQLENRIHDREEAMAFVQNRWKV"
     gene            complement(13803..15074)
                     /gene="thiC"
                     /locus_tag="PLA58_05835"
     CDS             complement(13803..15074)
                     /gene="thiC"
                     /locus_tag="PLA58_05835"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOR62351.1"
                     /translation="MTQLEKARQGVITGEMTLSAVQEGVSPEYIRQGIADGTIVICRN
                     VNHTAIRPLAIGLGLRIKVNANIGTSKDHNDLNLELEKLSVAIAAGADAVMDLSTGGH
                     LADIRKAVMEKSTVAIGTVPIYQAAVKMLGEKKAISDMTSDDLFAVIEENGRDGVDFI
                     TVHCGVTRQSVAAVEAQGRVLGIVSRGGSMTANWMRCNGRENPLYEEYDRLLDVARRY
                     DMVLSLGDGLRPGAIADATDQGQVAELILLGQLATRARAAGVQVMIEGPGHVPIADVE
                     ANIKLQKKICQGAPFYVLGPLPTDIAPGYDHITSAIGGAIAGAAGADFLCYVTPAEHL
                     RLPSLSDVRDGVMAARIAAHIADIAKGVPGAFERDRKMSECRRNFDWQGQVDVSIDPE
                     KTSALLEKSKSAEEEGCTMCGEFCAIKLGKK"
     gene            complement(15071..15898)
                     /gene="thiD"
                     /locus_tag="PLA58_05840"
     CDS             complement(15071..15898)
                     /gene="thiD"
                     /locus_tag="PLA58_05840"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOR62352.1"
                     /translation="MGKPIRVLCIGGSDSGGGAGIGADLKAVHACGCYAVFAVAAVTA
                     QNSRGVQSVFPVPPKWIAAQLDSVLGDIGADAVKTGMLMTPQAVDAVAKKIEQYRLKN
                     VVVDPVMIAKGGRALMTNRARQTLVKKLLPLADVVTPNIPEAEFLSRMKIRSVEDMKE
                     AAAVILDLGVKKVVVKGGHLPGRRPEGSVDILYDGKQYNEYAAKWIRTNNTHGTGCTF
                     ASILASHLARGENIYHAVAQAKIMTAQAIQYSLPLGRGHGPVNLLGIWQEKVRRKKQ"
     gene            complement(15899..16570)
                     /locus_tag="PLA58_05845"
     CDS             complement(15899..16570)
                     /locus_tag="PLA58_05845"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOR62353.1"
                     /translation="MIQNIVIVSGGRLGNVDFFRRKLNQTGDFLLICCDGGARHLAGT
                     GLAPDVLIGDMDSLLLEQLELYEQQGVKVIRYPAGKDFTDTALALDYALGLEPQRIQI
                     WGALGGRVDHALANLFLLVRAKEAGIAACLLDEYGETFVPVHEAVFENAEGCLLSLLA
                     LSSRVEGVTLEGFAYPLEEGTLELSESRGVSNIIVAKRARIHVRSGHLLVVRYWQKDV
                     FPEAD"
BASE COUNT         3669 a         4837 c         4316 g         3760 t
ORIGIN      
        1 tcaccttgtt cattgcaagg aagccgccag accaaaaacg agcccggcct tcgggtgacc
       61 gaaggtcaat tagcacttga gactgttttt gtaaaggaaa tattatgatt atttgtaatt
      121 tttggtaatc gtaagaaaac gcagaaataa ttaccacagc cccagaaaag gatccttttc
      181 aatcggttca acatttttta cgattgcccg accgcaagaa aagcagagaa tgttcatgaa
      241 tcttccctgc tcttctttca ggattcggtc tgaaaaccta cagtggaatg ttgttgtgct
      301 tcttttctcg ccttgcctct tttttattcc gcagagcctc cagcaggacg acgatgcgcc
      361 ggcgcgtctg gctgggcaga atcacctctt caatggcgcc cagagacgca gcgtaatagg
      421 gattgttgaa tttttcctcg taatccgcga tcagctccgc gcgcttcgct tccggatccg
      481 ccgccgcttt gatttcggcg cggtgaatga tgttgcaggc gcccgaagcc cccatcacgg
      541 cgatttccgc cgtcggccag gccatgacgt aatccgcccc cagctgcttg cagcacatgc
      601 cgatgtaaga cccgccgtaa gccttgcgcg taacgacggt gatcttcggc accgtggctt
      661 ccgaataggc gtgcaggagc ttcgcgccgt ggcggataat gcccgcccat tcctgatccg
      721 tgccgggcat gtatccgggc acgtcggaga acgtgagaag cgggatgttg aaggcgtcac
      781 agaaacggat gaagcgcgac gccttgtccg aagcattgac gttgagaacg cccgcaaaag
      841 acatgggatt gttggcgatg acgcccacgg gactgcccat gatgcggatc aaagcaacga
      901 tgatgttcgg cgcccactgt gcgtggagct caaacagttc actgttgtcg gcaaccgact
      961 tgataacatt tttcatgttg taggcgcgcg ccgctctgtc gggaatgatc ttgttcaatt
     1021 ccggacattc ccggtcgggg ctgtcggtcg ccactgcgac gggcagcggc gaatggcagc
     1081 tatccggcag gtacgaaagc aattccttga ttttccggat gcagtcctcg tcgttttcgg
     1141 tcacgacatg gcatacaccg cttttggagg cgtgtgccat cgctcctccc aggtcttcct
     1201 gcgagacttc ttctccgatc acggccttga tcacgtccgg ccccgtaatg tacatgtacg
     1261 aggtattctt taccatgaaa atccagtcgg tgagggccgg agaatacacg gcgccgcccg
     1321 ccgtcggtcc cataatcgcc gtaatctggg gaatataacc ggaagccagg gtgttgcggt
     1381 agaagatgcc gccgtagcct tccagagacg gaacgccttc ctgaatgcgc gctccgccgg
     1441 aatcgttgat ggaaataaac ggcttttgcg catcgagcgc catgtccatg actttccaga
     1501 ttttcttggc gtgcatttcg cccagggttc cgcctgcgct ggtaaagtcc tgagaagccg
     1561 caaagacgat ccggccgttg actttgccga agcccgtgat gacgccgtcg gcgggaattt
     1621 cctttttatc catgccgaac agggtggcat gatgcttgac gaaaagctgc acttcctgaa
     1681 agctgccttc atcaaacaac cggtccaacc gttcgcgggc cgtgagcttt ccaccgtcgt
     1741 gctgcttctg cacctgcttc gcgccgccca tctgcgtcaa tgcctgaacc ctggcgtcga
     1801 agtcccggag cttgccttcc gtcgtcgcct gtctgatatc gtcccccata gcgaatctcc
     1861 ttcgtgatct tttgaatcca ttgatcgttt cccgttttta tcttgaccgg gcttcgggcc
     1921 tacgttagga tcccgaacgg aaaaataccg gcccggctct gcgttttgga aacgtcggag
     1981 aagtgcttaa cctttttgag gcaaactggc aagtctgatt tgtacgctat gcgcaacttc
     2041 aaaatgattc tcgaatacga cggcgcggct tactgcggct ggcaaagaca gctcaacgga
     2101 cgttccattc aacaggtcgt ggaagaggcg ctggagcgga tcatcggcga aagcgtgacc
     2161 gtcatcgcct ccggaagaac ggacgcaggc gtgcatgcca tgaaccaggc agccagcttc
     2221 cggtctcaca ccggcctttc ggccaacaaa atttttctgg gcgtcaacag cgtcttgccg
     2281 gatgacattg ttgtgaagga cttgcaggaa gcgccgccgg atttcaacgc actcaaagac
     2341 gcaaaaggga aagtctattt gtaccggatc agaaatcagc gcctgcgtcc cgcgctgggg
     2401 cgccagtatg tctggcatgt gcgctacccg ctgaatcttg accgcatgcg acaggccgcc
     2461 tcgcggctca tcggggagca tgatttttcg tgcttttgcg cgacgggctg tgatattaag
     2521 gatcgcgtac ggctcctcac ggtcttgtcg atcgacaccc cggagaacgg cctgatggaa
     2581 atcacgcttg aatccagcgg atttttgcgc catatggtgc gcaacatcgt cggcacgctg
     2641 gtggatgtgg gccggggaaa aatcgcgccc gaacgcatcc cggagattct cgcatcacgc
     2701 gaccggaaac aggcgggaac ggccgcgccc gcctgcggtc tgtttttaaa ggaggtcctc
     2761 tactgaacca tgaaagtgct gttactcgga cacaagggaa tgctgggaaa cgatctgatg
     2821 gctcaattca gacaccggca tgacgtcatc ggcatggaca gggaagagat cgacatcacc
     2881 cgcgcgtccg aatgccgcag ggccgtcgcg gaaattcagc cgcagttggt catcaacgcc
     2941 gccggctaca ccaatgtgga cgcctgcgaa accgccaagg aagaatgttt cgcggtgaac
     3001 gccgaagcgg taaaaaacat cgcccaggcc tgccacgatc aaaatattac catcattcat
     3061 tacagcacgg actatgtttt tgacggaaac gccgatcacc cctacacgga agaagatccc
     3121 tgcaatccga tcaataccta cggggcctct aaattggcgg gtgaacacca tctgaagcgt
     3181 ctgacggaca attttattct gattcgaacc gcctggctct acggcgcgaa agggaaaaac
     3241 ttcgttggag cgattctgga ccgggccagg gagacgggaa aactgacggt cgttgacgat
     3301 cagatgggat ctcccacctg cacgcgcgat ctggcggcgg cgacggagct tctggtggat
     3361 aaaaacgccc gcggcatttt ccatgtgacc aaccgcggca gctgcacctg gtatgatttc
     3421 gcgcgcaaaa ttctgaagga agcccgcctg gatcaggtcg aattgtcccc gatgaaaacc
     3481 ggcgaattgc agcgggcggc cagacgaccg gcttacagtg tgctggggat gcaaaagttt
     3541 gtggccgcaa ccggcaagac catgcagccc tggcagctcg cgttttcgga ttattattcg
     3601 tttgtcacca gcaggacctg catgagcccg ccgggaacgg cttgataggg cggtgcgtcg
     3661 gaagaacccc ggcatgcgca cacgggaaca aggggttgca accccctgct ccaagcggat
     3721 ttgcggcaac cgatccaggc ggtcgaccgg cggaaggatt tttaaaatga attccagaag
     3781 gacgtatgat ataattacaa ccaatcccat caggatggag gtatcgccca tgcgaaatat
     3841 attcaagatt gttttctggg gtctgattcc cgccgcggcg ctgatgctgc agggctgcct
     3901 cgtttccaca ccgtccacgc tcatttcgcc ggaaatccgg tctttgtacg aaggggaata
     3961 taaaattgat ccctatatga agaaacatgt gccgctttcg acggccgtct tgccctttgt
     4021 cgatttgtcc ggaagcaagg aagggggaaa tgccatgcgg cggggcttct ataaccattt
     4081 cagttccctt cccttcaaag acatggaact ttacaaagtg gatgacctgc ttgcgaaggc
     4141 ggggttgacc gacgcggagg cagtccgtaa aacctctccg caaaaactgg gtgaaattct
     4201 gggtgttgat gcggttgttt tcggagaggt atccgacttc gacaaatatt tcgcggttct
     4261 ctattcgcag gttgccgtgg gcgcggaagt caagatgtac gatacaaaaa ccggcaattt
     4321 tctgtggagc ggcaagcaca aggtgcgcaa gcacgaaggc ggcgtctcca cgaacccggt
     4381 cggcattatc gccacggtga ttgcgaccgc cctgaatgtc cgcgatattc agctgctgcg
     4441 cgccaatgat gatctgttcc gcgacatggt caagaccatt ccgacgccgt ctctggccca
     4501 ggccaagcgt cctccggtca tttcgcttct cacgcaggac accaaggggc tgccgaaaaa
     4561 ggcaggcgat gagatcaaag tggtcattca gggaacaccg aacatgcagg cttctttcga
     4621 catcggccag taccggaaac gcatcgaaat gcaggaggtt gagccgggcg gttattacgg
     4681 cgtctataaa gtggtgccgg gggacaacat cgaaaaagcc atcgtcaccg gctacctcac
     4741 ggacgattcc ggaaacaccg cccactgggt ggacgcgatc ggatccgtca cgctcgacac
     4801 cactccgccg gataacccca caggcattac atcaaaggga aggaaccggc tggtggcgct
     4861 gcgatgggaa aagaacacgg cggcggatct ggccgggtat ctcatttacc gctccgacac
     4921 accgctgtcc ggctacgtcc agattggcaa gacggagttg aatgaatata gcgatgccga
     4981 tccgaatctg gcaaactccc ggacgtacta ttaccggatt tcggccgtgg atttcgccgg
     5041 caatgaaagc cggatggcgc aggcgcaggg catgccgatc gcacccgggc cgacgccggt
     5101 ttccgggatc attgaagcgg acaccacctg gtattcgggc gccagcccct acattctgga
     5161 gtccaatgtc acggttaaag acaaggcgct tttaaccatt gagccgggaa cggaaattca
     5221 gtccaaaggc ggagcgctga ttgtcgaggg gcggatttca gcacggggcg acaacgacag
     5281 catcatccgc tttgactcgg cggaggaaaa ccaactctgg ccgggcattt cgtttgccag
     5341 cgtgaaggac cgggaaaacc atctggcgtt tgtccgcgtg cgaaacgcgc gaaccgccgt
     5401 tttatgtcag gcctcgtcgc ccaccattga gaaatccgag tttacgcaaa accaggaagc
     5461 gatccggatt cagggagcct tttccaaacc gatcatcagc aagaatgcgc tttataaaaa
     5521 caagggcgtc gtcatttccg tcagcgatgg ggccgcgccg cgcattgtgg aaaacaccat
     5581 taacgacaac gacaataccg gattgatgct ctcttcctca gccgcggaaa tttcgcaaaa
     5641 cacaatcacc agaaacaaaa ataacggcat tgtggtcaaa ggcgcgccag ccgtcatttc
     5701 ccaaaacaac ctggtggata atcagccata caacctgttt ggcgagatga aaggcgctcc
     5761 ggcaaaggcg ctgaacaact ggtggggcag cgcaaaaatg gcggttgtcc tgtccggaat
     5821 ccacgggcgg gtcgatgtcg gctccgtgct ggacggaccg tacccgcagg gcaaggcgct
     5881 ggccgtgccg atcctggata aaaagctcga aggcgcgatc caggcggatg cctatctgat
     5941 tttatcacac agcccctacc gcgtggccaa agacgtggtc attgacggag gcgccaccct
     6001 ctacatcgaa cccggggtgg ttgtcgaatt cgaacaggga acgtccttca ttacccaaaa
     6061 cggcggcatg gtcgcgaggg gaaccaggga aaaaccgatt acctttaccg ccgcttcttc
     6121 ttcacccgct ccgggcttct atgtcagcgc cgtgcggttt tccgaatcca ccaaagtcaa
     6181 cagcgctttt gcctattgca tcgtgaaata cgcgacaacc gcttttgatg tttatacggg
     6241 atcgcccgaa atctcctaca gctacattgc gcatgcctcg cagagcgccg tttattgccg
     6301 taaagacgcc gccccggtcc tttcctacaa cacctttgcg caaaatcagg gcgaaggcgc
     6361 cgtcaaatgc gtgggctcgg ccaatccgag aatttttcaa aacaacttca tcgacaacgc
     6421 cgtggccgtg cagagctttt cgtcgattta catcgacgcc cgcaacaact ggtggggcag
     6481 cgctcctccc gatcccaaaa ttttctgggg agagaacatc aatacgaaac cgtggctgga
     6541 acaggccaac ccggaggctt ttacggaaag acgttgacac cggcaggtta tttgcctata
     6601 gtcccgcaga cttgaaaacg gagtgctgtg ggtgatttat ttccgcaacg tcagtttgtc
     6661 ttttctggat agacacctgt tcgaccagat ttcctggacg attcatccca aaagccgcat
     6721 cgggttggtg ggcgataacg gcgcgggcaa aacaaccctt tttcgtgcca tcctcgatga
     6781 ggtgcgcctc gactgcggcc tgatcgaaat ccccggccgg aagcagaaaa aaatcggcta
     6841 tcttccgcag gacttagtcg agctggaagc caccggcctg atcgaattcc ttcgccggaa
     6901 aagcggcatc gcccaattgg aagaagacct ccgcaccctg gagcggcaaa tctcacagat
     6961 tcaatccgac acgagcgaat acggcgagct ttcgcgccgc tatgcgcagg cgacggccga
     7021 attttccgcc cgcgacggat acgcctttga atcccgggcc aaacaaatcc tcaaagggtt
     7081 cggcttccgc gagagcgatt ttgacaaaac ctgcgacgtg ttttccggcg gttggaaaat
     7141 gcgcattctg ttgacttcca tcttgctttc ccaaccggac atcatgctgc tggacgaacc
     7201 gaccaaccac ctcgatacgg aaagcatgga atggctggaa agctacctga aggactaccc
     7261 cggcaccatt ctggtcgtgt cccacgatca tttcttcctg gataaaatcg ccacgcaaat
     7321 tgcggaactg gccaaccgcc gcattcacct gtattcgggc aactacacct attacctggc
     7381 agaaaaggag cgacggcgcg aagcgctgaa aaaagagatg gaactgcaaa aagcgcagat
     7441 caaaaaaatt gaagatttcg tggagcggtt ccgctacaag gccaccaaag cgtcgcaggt
     7501 gcaaagccgc gtcaaaatgc tggagaagtt cagggacatt cagccggaag gcagcgccaa
     7561 aagcgtcgtc atgaaattcc cggaaggcaa aaaaagcgtg aaggaggtgg tcgcggtttc
     7621 cgacctgggc cacgcctatg gcgatctgac ggttttttcc ggtttgaatt tttcgctttt
     7681 ccggggcgag aaagtcgcct tcgtgggcgt gaacggatcg ggaaaatcca cgctgtcaag
     7741 gcttttgagc ctctcggaaa agccttccca cggctctgtt acgtacggag acggagtgaa
     7801 catggctttt ttttcacagg aaagctctca aaatctgcat tatgcaaaca cgatctggga
     7861 agaagttcag gcggttccga cgtccgccga cgatcagacc aaacgcaacc tgctgggagc
     7921 gtttcttttt tcaggagacg atattttcaa acccgtcggc gttctgtccg gcggtgaaaa
     7981 atcacgtctg gcgcttttaa aaatcatgct gctcgacacc aacttcctgg tgctggatga
     8041 gccgaccaat catctggaca tcaaaaccaa agacgttttt caaaatgccc tgctccatta
     8101 ttccggaacg gtggccatcg tttcccacga ccgttacttc ctggatcgcc tggtcaacaa
     8161 ggtttttgag ctcaaggatg gggagatcag ggcttacgcc ggcaattatt cctactttat
     8221 tgaaaagcgc gccgagcgga cacaggacac tggacagcag catcgtcgcg accgggcggc
     8281 cgtggacagg acgcttaccg caaaagacaa aaaaagactg gaagcggaag aacgcaaccg
     8341 cctgtcgaaa atcaaaaacg gtttaaagag gcagctggcg gaggtggaag caaaaatcac
     8401 agaccttgaa tcccgaaagg ctgccaatga aaattccctg tgtgatcctc aaacacacaa
     8461 agatgcaggt aaaatcaaag agcttcagat cgatctgaag gcctttgaaa aagagcttgc
     8521 tcaagcctac cgcctgtgga cggaattgag ttgcaaactg gaagagactg attccgccgg
     8581 ctccgcctga tttcaaatca agatcaagcg ttcccttttt ggcgtcgttg atttccttca
     8641 tggatgtgca gggaacggtt taaaatggtt ccctgcgagc tgccgtgctt ttcatgcccc
     8701 gtccagaccc gagcgttcca tgtgctgata ccaggtggcc ggatcgacca gaaattcttc
     8761 cgtgtctttc agcgacagca ggtgctgacg ctccacttgc gcgagcatat cgaaaaacgc
     8821 aacctctttg gcatccgtgc actgttttgc cagcttggaa tacagggcca ccccgcgcgc
     8881 ctcgaaatct atcgccgtgc ggaccgcctt gagttcatct tccgtgccgg taatgcgggc
     8941 ggactttttc ccggacatgc cctgcaacac actcccggct gaggacggct gcactttcag
     9001 gggaatcgtg gccggccatt tctttttctc cacccatttc gcatggagtt ttttcaacat
     9061 ttcgtaatgt tctttttcct catcggcaat ctgtttgaac atgtttttcc cggccatgtt
     9121 cctggtcttc cgtgcctggg ccagataaaa ctcacgctcc ttttcttcat tttccaaagc
     9181 aaaggccagt gcgttcattc ttttatccat aaaaccccct tcggcggatg atcgtatttg
     9241 cgcggtatta tagaagatgc tgcaaattca ggcaatacgt ttttttaagg caggctattt
     9301 caggaagccc tgaatgatcc ggttcacggc ctggtcatag gtcaatccca ggctcatcag
     9361 tttggtcagc tgatcattcg caatcttgcc gatcgaagct tcatgggtca attccgcgtc
     9421 gggatggccg gccttcagcg acggcatggt ctcattggtg ccgttgtcca tgatgatcgc
     9481 gtcgcactca atgtggccgt agcatttgtt ttgcgcatcg atggtcgcgt aaaatatctg
     9541 cttggaattg cccttcatca ccgagcgtga aatcatattg gcccggctcc ccgcgccttt
     9601 caaaacgata ttattggtgg atacagccgt ttgttcgccg tcggtcagaa ctctctcggt
     9661 gatcagcagg aggctgtccg gccccagaac cgcttcattg atgcggttgg cttcatccac
     9721 accgccgatc tgcgtcaatt ccatttgcgc gtgcgcgcct tcggccagaa acactctggt
     9781 ggttgggttc agcgtccggc ggcccgcgcc ttctccggac gcgtaatgtt tttcgacata
     9841 cgtcaccttc gcatttttcc cgaccttgaa ctcgtgaatc cccgcgtgtc cctccggttc
     9901 ggacgagccg cagtgaatcc cgcagccggc aataatcgtg acctcggcgt cgtcgccgac
     9961 aatgaaggca ttataaacca cgtcatacag accggcctgg cttaaaatga cgggaatatg
    10021 aaccgattca tttttcgttc ccggtttgat tttgacgata atgccggttt catcggcatt
    10081 ggtttcaata ttgatgttgg ccgatacatg acggcttaac agcttgccgt tttttcgtat
    10141 attgtaggcg cctttcggca attcctccag ttcggcgacg gttttcaata aggttttatc
    10201 gatagcatcc agcatcacga tctccttcac agctgtcccg atacgtacag acgctcaaat
    10261 catccagaag cggcatggcg ccttccaggc ttccgttata aacgatcttg cctgaatgaa
    10321 tgatcatcac cacgtccgcc gcctctaaaa aggctttatt atggctgacc acgatggtgg
    10381 tggtgcaatt ttcagccagc tcctttttca ggagactcac catcgggccg atcgtccata
    10441 aatcgattcc cgtgtccggc tcatcgtaaa tcgccagttt gggattggcg gcaatggtcg
    10501 tcgccagctc aatttttttg atttcaccgc cggacagcct ggcatccact tccttatcca
    10561 gaaaggacag ggggcaaatg cccacccggc cgagaatctc cagcagcttg ctttcatctt
    10621 caatgccggt ggccaccgtc agcaaatcac gaaacgtaat gcctttaaag cgggcgggct
    10681 gttgaaagct gtacgcgatg ccggctttgg ccctctccgt gatcgacagg gtggaaatat
    10741 ttcgtccatt gaaaagaatg tcgccgctgg acagcgggtt gatgcccatg attaactttg
    10801 ccaatgtggt cttgccgctg ccattgggcc cggtgatcac ataaaatttc ccgtcgtcaa
    10861 acgtgaagtc aatcccgtca ataatgttgc gcacaccgcc gcttccctca ctgtcggata
    10921 cggtgaaata cactttgttt agttccaaca tggaatctgc cttttctcct gtaatttttc
    10981 cggctccgat atatgtcgct ttgcggtttc tgtcaatagc cagctcgtga tccgttcatc
    11041 acaaacgccc ccggaccgat gctgccgctg tcaatgctga aaatgaattc tcggatgatg
    11101 aatcccctct gaacgcagaa ggtcatcaga aaagaatgga acggagcaaa tatttaaaga
    11161 gcagggaact tgcgtcctcc tgctctttaa atgtggatgg ccgatcagta ttacttcttg
    11221 gctgctttct tcacgacttt ctttttcgcc gcaggcttct ttgccgcaac ttttttcttc
    11281 gccgccggtt tctttgctgc ggccttcttc acgactttct ttttcgctgc cggtttcttt
    11341 gctgcggcct tcttcacgac tttctttgct gttgctgcca ttgggtttag cctcctttaa
    11401 ttgagtttac ttcttccttg cagaatccgt caccttacaa atgtggctta atattacttt
    11461 atttttttct ttcggtcaac aaaaactata ataaatcttt acttttttta ttttttaaaa
    11521 aagatttttc ggatgaaaag actgtgaaaa taattctggc aagagcgccg cggtaaaata
    11581 cgcaacggga ggaagccgtg aaacccttta atgaagaggc tttcaggctc ttcggatcag
    11641 agatatgttt tcgctccgac gaagcgccgg gcatagtaat cggaggagag cgcgtcgatg
    11701 cggatttttt ttccgctgga cggcgcatga ataaacctgc cggcgccgat gtaaatgccc
    11761 acatgcgaaa cttttccgcg tccgcgcgtg tcgaaaaata ccagatcgcc tttctgcaga
    11821 tcctcccgcg caatcgaatc tcccgcgtca tactgcgttc gtgaatgccg cggcagatcc
    11881 agaccgttga gctgatagac cgtcatggtc agtccgctgc agtcaaagcc gctgtcggcg
    11941 gaaatgccgc cccacagata cggcacgccg ataaaatcgt gagcggtttt caccagcgac
    12001 tcccgcaaat aatccgttcc gtattggctg cgtttggccg ccgcggaatc ttccggcaat
    12061 acgatatagt attcttcaat cacgcccgcc tgcctgagcg actgcgcgcg gagcagcgcc
    12121 gactccttcg tcgtaaagtt tccgaacctc accttgaaca atccgtcggc cgccctgaag
    12181 taagtagcgg gcagtccctg acctttgagc ttctccgtca gacgcacggc attttccacc
    12241 cgggcgaaag cgcccgcctg aatcgtgtgc cccatctggg caagctgctt tttctgcggc
    12301 ttgggcgcgg cgggatacac ttttgcttcg cggcccgcac aggcctgcat cagcaacccc
    12361 atcagcagta gaaccgccag tctgaagcag atttttttca tcttccctta ttccacgcga
    12421 cgccgtgtta cactttccag cggttttgca caaacgccat cgcttcttcc ctgtcatgaa
    12481 ttctgttttc cagctgctct tcctccagcg cccgcagcat ttcgcccatc cgttttcccg
    12541 gagcgtaccc catcgccaga agatcatctc ccgtcagcaa acgcggcgga tgcagctctt
    12601 ccggctccgc cttttgcagc tgctcccgac agaactcata atggtccagc atcccgtggc
    12661 tggccagaca atccagccgg tgcaactgca gatgcagatg aaaattcggc atccgcaaaa
    12721 atcgcttcaa gcgggcgggc cgcattttct gcacgttcat aaaagccatg tgccggccaa
    12781 tcaggtccag aaccttttcc cggagagttc gtgagaaccg cagccgacgc atcacatcgt
    12841 cagcaatgga ttctccccgc ttcacgtgcc cgtaaaaatg cacgccgttt tcgtcttccg
    12901 tccttgtgac gggcttgccg acgtcgtgca aaagcgctcc ccaggccagt gccgcatccg
    12961 cttcgggttt gccttctccc gacagcatct ccatcatgat cagtgtatgc cgccagacat
    13021 cgccttccgg atgaaaacgg ggcggctgcg cgaccccccg catccggtcc acttccggca
    13081 gaacttgctt cagcaggccc gtctcggcca tccattccaa ccccctgcgc gctccgctcc
    13141 ttgtgaggat tttattcaat tcctcctgta cgcgctcggc gctgatttcc cggattttca
    13201 ccgcatggcg cgcaattgcg tcccggacgc cgtcatcgag atgaaaatcc agattagcgg
    13261 caaagcggat tgcccgcagc attcttaaat ggtcttcatt gaaacgaacc cctggatcgc
    13321 cgatggtgcg gatcattttc tttccgatat cggcgcggcc gcctacgtaa tcgatgatgg
    13381 cgttcgtctc aggatccatc aacaggccgt tgatcgtaaa atcccgccgc aggacgtctt
    13441 cccgggccga tgaaaagcgc acctgcgtgg ggcggcgtcc gtcttcgtac ccctcgtcgc
    13501 tgcgaaaagt cgccacttcg taggaaaaac cgccctcgcg gaccaccacc acgccgaatt
    13561 tggcgcccac ggcaacggtg cgttcaaaaa gacggatgac ctgatccggc agggcggatg
    13621 tggcgatgtc gtagtcgccg ggagacacgc cgcgaacata atcccgaacg cagcctccga
    13681 caaaataggc ttcaaaaccc gcccgccgca gaccttcgac aacccgcttc gcgccgcgat
    13741 gcttttcgat ggaatgatcg tccgccgtcc ggatcaatct tgcttcctcg tgatgacttg
    13801 gtctatttct ttcccagttt gatcgcgcag aattcaccgc acatggtgca gccttcctcc
    13861 tccgcgcttt tgctcttttc caggagcgcg gatgtttttt ccggatcgat ggacacatcc
    13921 acctgcccct gccagtcaaa gttcctgcga cactcggaca ttttccggtc ccgctcaaaa
    13981 gcacccggaa cgcctttggc aatatcggca atatgcgccg cgatccgcgc ggccatcacg
    14041 ccgtcgcgga cgtcggaaag cgaaggcagc cgcaaatgtt cggccggcgt cacatagcac
    14101 agaaagtcgg cgccggcggc gccggcaatc gcgccgccga tcgcggacgt gatatgatcg
    14161 tagccgggcg caatgtccgt cggcagcggc cccagaacat aaaacggagc cccctgacag
    14221 attttcttct gcagtttgat gttcgcttcc acgtcggcaa tcggaacgtg tccggggcct
    14281 tcaatcatga cctgcacgcc cgcggcccgg gcgcgggtcg ccaattgccc caggaggatc
    14341 aactcggcaa cctgtccctg atcggtcgcg tcggcaatgg ccccgggccg caggccgtct
    14401 cccaggctca gcaccatgtc atagcggcgg gcgacgtcca gaagccggtc atattcctcg
    14461 tacagagggt tttcccggcc gttgcagcgc atccagttgg ccgtcatcga accgccccgg
    14521 ctgacaatgc ccagcaccct gccctgcgcc tccacggcgg ccacgctttg gcgggtcact
    14581 ccgcaatgca cggtgataaa atcgacgccg tcccggccgt tttcttcgat caccgcaaac
    14641 aggtcatccg acgtcatatc ggaaatcgct tttttctctc cgagcatctt taccgccgcc
    14701 tgataaatcg gcaccgtgcc gatggcgacc gtggattttt ccataacggc tttgcgaatg
    14761 tccgccaaat ggccgcccgt ggacagatcc atcacggcat ccgcccctgc cgcaatcgcc
    14821 acggataact tctccagctc cagattcaaa tcattatggt ctttggacgt tccgatattg
    14881 gcgttgacct tgatgcgcag cccaaggccg atcgccagcg gccgaatggc cgtgtggttc
    14941 acgttacggc agatcacgat ggtcccgtca gcgatcccct gacgaatgta ctccggcgaa
    15001 acgccttcct gaaccgcgct gagcgtcatc tccccggtaa tcacgccctg acgggctttt
    15061 tccaactgtg tcattgcttt tttctcctta ccttttcctg ccatatgccc aaaaggttca
    15121 ccggaccatg acctctcccc aggggcagtg aatattgaat agcctgcgcc gtcatgattt
    15181 tcgcctgcgc cacggcgtga taaatgtttt cgccccgcgc gagatgggac gccagaatcg
    15241 aagcaaaggt gcagccggtc ccatgggtat tgttggttcg tatccatttt gccgcatact
    15301 cgttatattg cttgccgtca tagagaatat ccacgctgcc ttccggcctt ctcccgggga
    15361 gatgcccccc cttgaccacc acttttttca cccccagatc caaaatgacg gcggcggctt
    15421 ctttcatgtc ctccacggaa cggattttca tgcgcgacaa aaactcagct tcgggaatat
    15481 tcggcgtcac aacgtccgcc agcggaagca gctttttcac cagcgtctgc cttgcccggt
    15541 ttgtcatcag ggcgcgtccg cccttggcga tcatcaccgg atccaccacg acatttttga
    15601 gccggtactg ctcaatcttt ttcgcaacag catccaccgc ctgcggggtc atgagcatgc
    15661 cggttttgac cgcatccgcg ccgatatccc ccagcaccga atccagctgc gccgcaatcc
    15721 atttcggcgg caccggaaaa acagactgca ccccccgtga attttgcgcg gtcaccgcgg
    15781 ccacggcgaa taccgcataa caaccgcagg catgaaccgc tttcaggtcc gcaccaattc
    15841 ccgctccgcc tccggagtct gatccgccaa tgcataaaac ccgtatcggc ttccccattc
    15901 agtccgcctc cggaaagaca tctttttgcc agtaacggac aaccagcaga tgacccgagc
    15961 ggacatgaat gcgggcccgt ttggcgacaa tgatgttgct cacgccgcgg gattcggaaa
    16021 gttcgagcgt tccttcttcc agtggataag cgaatccctc cagcgtgacg ccttccacgc
    16081 gggaagaaag cgccaggagg gacaagagac agccttccgc attttcaaag accgcctcat
    16141 gaacgggcac aaaagtttca ccgtattcat ccagcaggca ggcggcgatg cccgcttctt
    16201 tggcccggac cagcaaaaac aaattggcca gggcgtgatc gactcttccg cccagcgctc
    16261 cccagatctg aatcctctgc ggctccagtc ccagggcgta atccagggcc agcgccgtat
    16321 cggtaaagtc cttgccggcc ggataacgga ttactttcac gccctgttgt tcataaagct
    16381 ccagttgttc caataagaga gaatccatgt cgccgatcag aacatccggc gccagtccgg
    16441 tgcccgccag atggcgcgcg cctccgtcgc agcagatcag caggaaatcg cccgtctggt
    16501 tcaatttcct gcgaaaaaaa tccacgtttc cgagacgccc cccgctgaca ataacaatgt
    16561 tctgaatcat ggatcatcca aa
//
LOCUS       DAOSRM010000044        16432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3631_length_16432_cov_110.890700, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000044 DAOSRM010000000
VERSION     DAOSRM010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 16432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16432
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3631_length_16432_cov_110.890700"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLA58_05850"
     CDS             <1..317
                     /locus_tag="PLA58_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOR62354.1"
                     /translation="IINVFSLKSTFAVKVGIYDDPVVVKIRFSPKQALYIQERKWCKN
                     QDIEKCADGSLHLTLRTSGWYDVQKWILSFGADAELLEPEDKRKEIMEEISRTAELYR
                     SD"
     gene            563..1597
                     /locus_tag="PLA58_05855"
     CDS             563..1597
                     /locus_tag="PLA58_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOR62355.1"
                     /translation="MVQLKFDQKVCASCRSADCLLKCQYITFAGHKEAHGEMMKVMKG
                     KDSRVLTECVTCYACEEYCTRGNHPFYLISEKREEKGMFTAPRPITTQWKNMTSMQGK
                     YLVGNVKDTALSCCYLPELGQLGTGEIFKDVASAQVFGAEFMCPAVHSHFARMSVIKE
                     RLPMVIENYRKLGVKEVICMHDECYGTLTSIASAYGIEVPFTPIYYLDFVLQRMKELK
                     DRIKPLGITVAYQRSCSNRLIPDKLPVVKKIFQLIGVKAPKRKYQGENALCCAEIIRS
                     VSGYKLADDVQKRNIDDMLAAGAEYCVFNCPACQTSLSEKVTRRGLKPVHIIDLCKMA
                     IGEKEREAVS"
     gene            1594..2976
                     /locus_tag="PLA58_05860"
     CDS             1594..2976
                     /locus_tag="PLA58_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOR62356.1"
                     /translation="MSDIRKSLVEIVGERGVSDAPEELWFYSRDPGVLTPHKPDYVVA
                     PKTTEEVQKIVQLANQENIPVVPMGNGMSLAGLVIPLKGGIVLDMKRMNKILEVNAMA
                     RYVVVEGGTSQGALKAYLQKHHPTLRHSIPDAPSTTTIAANVSLHGQGRLTNQYGFNS
                     DMVTGLEVVLPTGEICRIGSPSIGPYWISKGPTLPDLSGLFLGWLGCTGVITKVGLKL
                     YPNKKIRDAELFVTDRVDLIPHILYKLTHLEMLEDINVWFQPKPLMFMGNYHITVFFT
                     GDTEEEIEFKRKMIWDCLQEYMDSKDGGFMSISYMKPMLLEMPGRNIADFADVPKGGG
                     FEYSGPIAPIEHFPQYAAKVVELAAKYNVLYASTARLISGGHAMMYSISFAFNRNDKA
                     MMERVEKALDEATSFALGMGGIPWKPNFKEQKMLLQKMEPNARKLLLMIKKNLDPQGI
                     MNPGNWEVNA"
     gene            2973..4148
                     /locus_tag="PLA58_05865"
     CDS             2973..4148
                     /locus_tag="PLA58_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOR62357.1"
                     /translation="MTYIRHIEHENIVHRCFRCGYCKFPSDYSDFNCPSYKAFGWDTY
                     SPGGRMWLTRAWLSGEIETSARFAEIMFSCVACDNCKDQCVFPRFKDFLPDIFQETRA
                     ELVGEGRIPPGVRDYFKAVAVSGNPYKLPQSQRGDWAKGTGLKPYTNQKYLFYAGCVG
                     SYDEVGQKMARSVGKMLVDAGLSVGILGSEEGCDGNDVRALGEMGLFAQLAQANIETF
                     KAKGVREIITLDPHALNTFRKDYPVIGGVFQVRHYTEVLAQLMKKKKIKLKAWPVKIT
                     YHDPCYLGRHQNIYQAPREILKAIPKAEYVEMRRSGPDAFCCGGGGGNFFTDILGTAE
                     DSAARVRVREAAETGASILAVACPNCAKMLSDAVKMEQLENRIEVLGIAEILERARK"
     gene            4167..4694
                     /locus_tag="PLA58_05870"
     CDS             4167..4694
                     /locus_tag="PLA58_05870"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOR62358.1"
                     /translation="MNREEMLEEVYKRAKAYELRGGNCSQCSLAAIFEALGVEDENVV
                     RAATGFADGVGLSGDGHCGALSGGTMAISYFFGRKKEDLGRMGKQIKALLLAKKFHEQ
                     FMKEFSTCRCRDLQVKQFGRFFNLYDMEDMKAALAAGMPEGCSTLVGKAARMALEIIL
                     DEQARQEKKAELPVV"
     gene            4748..6199
                     /locus_tag="PLA58_05875"
     CDS             4748..6199
                     /locus_tag="PLA58_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR62359.1"
                     /translation="MIRSISESPLYPIVNPKSIAFFGASNTFMRMGSIMLSSMQALGY
                     EGKIIPIHPKEKVVRGLAAYPSILDVPEIPDLAIIVLPTEIVCQTLDDCGKKGIRHAI
                     VVSGGFREAGPEGAALQKELEAIARRHGIRFLGPNCLGVANLHLKLNPTPIKADCKAG
                     FVGLASQSGSFITQMFNYLHRHGMGFSAALSVGNEASIDIVDCLEYLGACPHTKVITL
                     YIEGINRGSKFVEIAKAITPRKPIVALYVGGSEAGKQAGLSHTGSLSGPNEIYDGIFK
                     QCGIIRAKTLTELFDYALALGTLPRPGGNRIVIQTHSGGPGATAADACSRVGLKLSRL
                     SPETVKKLKPLIPKTASTANPVDMTFSKNHADDYFNIPNILLSDSETDMLLAYFVSPG
                     IFLERILGEMGVEENSINSEMDKLIGDFAQKFVSLTQAHPDKPIVGFTYRSLQEKMVR
                     TLLDCGVPVYQDPERAARSLAAVREYYERRGKM"
     gene            complement(6458..6811)
                     /locus_tag="PLA58_05880"
     CDS             complement(6458..6811)
                     /locus_tag="PLA58_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62360.1"
                     /translation="MSLVARHLEANGLPTVVMGCARDIVEECGVARFVFTDFPLGNPS
                     GKPGDAAMQQEILGTALELLERAWMPRTTVQTPYQWPDDAWRENFMRVDASNREELAR
                     QGKERRDLQARLKKS"
     gene            complement(6830..7333)
                     /locus_tag="PLA58_05885"
     CDS             complement(6830..7333)
                     /locus_tag="PLA58_05885"
                     /inference="COORDINATES: protein motif:HMM:NF019003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HOR62361.1"
                     /translation="MDQLRPYRSFVSYIDCSRTYYAAQGYDKPYAWPHYDEVPFAPPA
                     KPLSQCRVGLVTTAGLPKPADPMEAMLYRREMYAQPAWPPPASLYTDDLFWDKKATHT
                     RDVDSFLPLTHLASYAASGRIGSASPRFYGVPTDYSQGRTIERYSPQILNWCREDGVD
                     AVILLAL"
     gene            complement(7402..8502)
                     /gene="queG"
                     /locus_tag="PLA58_05890"
     CDS             complement(7402..8502)
                     /gene="queG"
                     /locus_tag="PLA58_05890"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOR62362.1"
                     /translation="MRTDDARHNGQRGYRLSNALFAEIDRVVDAGMWAVAGLRNGTKE
                     MKAIGAGNPFPSKDLENPALTAAAIKEEALRFGFDGCGISRAEALDEDAARLQKWLDR
                     GYHASMAYMGRHAHKRVDPTRLFEGARSVISVLLNYYPSGTQTDPRAPVLAKYAYGKD
                     YHTVVREKLNRLLEWILSGSAPVHGRAFVDTAPLLEKAWAARAGLGWIGKNTCLISPG
                     KGSFCFIGTLLVDIPLQYDNPVDDLCGDCTFCIDACPAKALVAPRTLDAGRCISYWTI
                     ENKAAVGEAFGDRLANRVFGCDICQDVCPWNRHAVPHREKDFDAPPGLLEMTREDWFN
                     LNEEQYGRLFQHSAVKRVGFEGLKRNLDRIVD"
     gene            complement(8532..9365)
                     /gene="mtgA"
                     /locus_tag="PLA58_05895"
     CDS             complement(8532..9365)
                     /gene="mtgA"
                     /locus_tag="PLA58_05895"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOR62363.1"
                     /translation="MQKWKKIILWSFLAVIAFLILDVARYFFYPDVAGLQKNRPGKTA
                     FMEYREEIWKEKGIKKKISASWVPLSGVSPYVMKAVIIAEDDKFWSHEGFDFDAMQKA
                     LEKDIQKKKFKAGGSTISQQLAKNLYLTPAKNPVRKIKEAILTWRIERQLSKRRILEL
                     YLNVAEWGDGLFGVETAARRYYGKSAAGLGPREAAQLAAALPNPRRFRPDGSSRYAAN
                     RAERIYQIMVRRGIVIEEYDEVLKDEGGEEEKAPEPASPVPETSAAGEPAQTEAPAVV
                     P"
     gene            9622..10560
                     /locus_tag="PLA58_05900"
     CDS             9622..10560
                     /locus_tag="PLA58_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOR62364.1"
                     /translation="MMQRRTMISWGLFALFLLFCFQIDAHAVQYKVKKGENLSLISRR
                     TGVSVAEIQKTNHLNSNIVKPGQILSIKEKNSPSPVAAKSKTHPTSSYIVKRGDTLPL
                     IAKKTGVSALQIARLNHVTTKSLRAGQKLIIPREEPPAKAPVEEEEDLLEEEDEGLTD
                     DASGQIACEEKVSEELLGKWGSADERKLFIRVATGFLGAPYRLGGATVKGIDCSAFVR
                     KMYEFFDITLPRTAREQSTVGMQVGRDELVEGDLVFFRTKKPIGHVGIYIGNNEFVHA
                     SYKAKAVRIDSLDRPYFQKRFQRAVRLKGLDENGGT"
     gene            10749..12683
                     /locus_tag="PLA58_05905"
     CDS             10749..12683
                     /locus_tag="PLA58_05905"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOR62365.1"
                     /translation="MGKNIVEKILAGHLVYGALAPGGEIGIRIDQTLTQDATGTLAYL
                     QFEAMNVPRVRTELSVSYIDHNTIQIGFENADDHRYLMSVAQKFGIVLSRAGNGICHQ
                     VHLERFGRPGKTLIGSDSHTPTCGALGMIAIGAGGLDVAQAMAGEPFYLACPSVVKIN
                     LEGRLSPWVSAKDVILKVLEIFSTKGNVGTVFEYGGRGLATLSVPERATIANMGAECG
                     VTTSVFPSDDLTRQFLRSQEREADFIELKADTDAVYARVETIDLSRIEPLAAKPHSPD
                     NIATVRSLAGIPVQQVCLGSCTNSSYKDLVTVAKILKGRIAHPDVSFILAPGSRQVLD
                     NLARDGYLASLIASGARLMENACGFCIGNSQSPQSGAVSLRTSNRNFPGRSGTLDADI
                     YLVSPETAAAAVITGRFTDPRDLAMEYPDARTPEKLVIDDSLILRPDDADRNGNIYRG
                     PNIGAPPVNTPLPDTLAGPVTIKVGDKITTDHIIPAGARMKYRSNIPRYAEFVFENVD
                     ATFARRALANKEAGRHNIIVAGLSYGQGSSREHAAICPMYLGVRAVIAKSLERIHAAN
                     LINFGILPLAFADEKDYDTLGEGDELEIAGLRTAVAGGESLTVANKTKGTSFAVRLSL
                     SPRQKEMILAGGAFNVRSPK"
     gene            12716..13933
                     /locus_tag="PLA58_05910"
     CDS             12716..13933
                     /locus_tag="PLA58_05910"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOR62366.1"
                     /translation="MTQKIKVKTTLVEMDGDEMTRVMWRMVKDKLLFPYLDMDIDYYD
                     LHVKHRDDTDDRVTVDAARAVQKYGVGVKCATITPNEDRVKEYNLKKAWKSPNGTIRA
                     LLDGTVFRKPILVNNIKPGVTAWKKPITIGRHAYGDVYDNFEMNIPGAGKLEVVFTPA
                     DGGAPRRELVKNFPSAGIVQVNHNLEASMRSFARACFTYALSEKIDVWFSTKDTISKK
                     YDAGFRDVFDREFAEHWKDRFDAAGIEYFFTLIDDAVARIMKTEGGILWALKNYDGDV
                     MSDMIASASGSLAMMTSVLVSPSGWYEYEAAHGTVQKHYYKHLKGEETSSNSMALIFA
                     WTGGLRKRGELDGTPDVAGFADNLEAAALETVESGVMTGDLLLLAEKRPTNRKVNTEG
                     FIDAIAQNLKKKL"
     gene            14177..15229
                     /gene="rlmN"
                     /locus_tag="PLA58_05915"
     CDS             14177..15229
                     /gene="rlmN"
                     /locus_tag="PLA58_05915"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOR62367.1"
                     /translation="MSKRNENSLPNLSDFTLPELEEFIAASGKEKYRARQIMKEIHQF
                     GCLSFDAMTTLARDFRKTIAQTARIYRPSMVKIQKSQDGTQKVLLGLEDGLFVESVLI
                     PGKNHWTLCISTQAGCAMGCAFCLTARQGFKRNLTPAEIVGQVVTIRKETPEGEAVGN
                     IVLMGMGEPLANYENTVKAIRILTSDFGLGFSSRRITVSTCGLARQILQLGSDICINL
                     AVSLNAHDDETRSRLMPVNKKYPLATLLDACKKYPMPGRRMLTYEYILIDGVNASRQD
                     AQKLTLLLADQRCKINLIVFNEYPGSPFRAPSPHVVSDFQQVLLDRHFTAMLRKSKGS
                     DILAACGQLSGTYTTG"
     gene            15573..15911
                     /locus_tag="PLA58_05920"
     CDS             15573..15911
                     /locus_tag="PLA58_05920"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOR62368.1"
                     /translation="MMEYKGYIGIVAYDDQARIFHGDVINTRDVITFQGKSVREIEKA
                     FKESIDDYLSWCAKDGVAPEKPYSGKFNLRLSPDLHREVAVTAKKLNISINSFVERAL
                     IDEINTHRAT"
BASE COUNT         3902 a         4723 c         4459 g         3348 t
ORIGIN      
        1 atattattaa tgttttctca ctgaaatcca cgtttgctgt caaagtcggg atttacgacg
       61 atccggtggt tgtcaagatt cgcttcagcc cgaaacaggc actctacatt caggaaagaa
      121 aatggtgcaa aaatcaggat attgaaaagt gcgcggacgg atcacttcac ctgaccctgc
      181 gcacatccgg ctggtatgat gttcaaaaat ggattctttc ctttggcgcg gacgccgagc
      241 ttttggagcc tgaagacaag agaaaggaaa tcatggaaga gatcagccgg acggcggagc
      301 tttaccggtc agactaaaat ccccgatggc tcccgccgtc ggggccagct ccaatccttg
      361 cggaagaatt gatcagaaat aatcaactgg ccctctggaa agcaaatgtg atatgaaggt
      421 ggccattcat ttcaatagcg ggatgcaatc tcaacgcgcg ggacaaaaac cgtcaggaag
      481 cgggatgaat gcttgacggg cgcgggcggc tcaagcgggt tccactgacc ccatcaacat
      541 cgaacatcga gggaggtgct tcatggttca actgaaattc gaccagaagg tttgcgcaag
      601 ctgccgctcg gccgactgtt tgctcaaatg ccagtacata acgtttgccg ggcacaagga
      661 agcgcacgga gaaatgatga aagtcatgaa gggcaaggat tcccgcgtgc tgaccgaatg
      721 cgtcacctgc tacgcctgcg aggaatactg cacgcggggc aaccatccct tttacctcat
      781 ctcggagaag cgtgaggaaa aaggcatgtt caccgcgccg cgccccatca cgacgcagtg
      841 gaaaaatatg acgtcgatgc agggcaagta tctggtgggc aacgtgaagg atacggcgct
      901 ttcctgctgc taccttcccg agctggggca actgggaacg ggagagatct ttaaggacgt
      961 cgcctcggcc caagtgttcg gcgcggaatt catgtgcccg gcggttcatt cccacttcgc
     1021 ccgcatgtcg gtcatcaagg agcggctccc catggtcatc gagaactacc gcaagctggg
     1081 cgtcaaagaa gttatctgca tgcatgacga atgctacggc acgctgactt cgattgcctc
     1141 cgcctacggc atcgaggtgc cctttacccc gatttattat ctggattttg ttttgcagcg
     1201 catgaaagaa ctcaaagaca ggatcaagcc gctcggcatc accgtcgcct atcagcgctc
     1261 ctgctccaac cggctcattc ccgacaaact gccggtggtg aaaaaaatct tccagttgat
     1321 cggcgtcaaa gcgcccaaac ggaagtatca gggcgaaaac gcgctgtgct gcgcggaaat
     1381 tatccgctcc gtttccggct acaaactggc cgacgacgtg cagaagcgca acattgacga
     1441 catgctggcc gcgggcgccg aatactgcgt tttcaactgt ccggcctgcc agacgtctct
     1501 gtcggaaaaa gtgaccaggc ggggattgaa accggtgcac atcatcgatc tgtgcaagat
     1561 ggcgatcggc gaaaaagaaa gggaggctgt ctcatgagcg atattcgaaa atccctcgtg
     1621 gagatcgtcg gcgagcgggg cgtctccgac gctccggaag aactctggtt ttattcgcgc
     1681 gatcccggcg tgctgacgcc gcacaagccc gactatgtgg tcgcgccgaa aacaacggaa
     1741 gaggttcaga aaatcgttca actggccaac caggaaaata tccccgtcgt cccgatgggg
     1801 aacggcatgt cgctggcggg tctcgtgatt ccgctcaaag gcggcatcgt tctggacatg
     1861 aagcggatga acaaaatcct ggaagtgaac gccatggccc gctacgtggt cgtcgaaggc
     1921 ggcacctccc agggcgcgct caaagcgtat ctgcagaagc atcatccgac gctgcggcac
     1981 agcattcccg acgccccttc gactaccacc atcgccgcga acgtctccct gcacggccag
     2041 ggaaggctca ccaaccagta cggcttcaat tcggacatgg tcacggggct ggaagtggtc
     2101 ctgcccacgg gcgagatctg ccggatcggc tcgccgtcga tcggccccta ctggatttcc
     2161 aaagggccga cgctgccgga cctgtcgggg ctcttcctcg gctggctggg ctgtacgggc
     2221 gtcatcacaa aggtcggcct taagctttat cccaacaaaa aaatccgtga cgcagaactg
     2281 tttgtcacgg accgcgtcga tctgattcca cacatcctct acaagctcac gcacctggaa
     2341 atgctggagg acattaacgt ctggttccag cccaaaccgc tcatgttcat gggaaactat
     2401 cacatcacgg ttttcttcac cggcgatacg gaagaagaaa ttgaatttaa acgcaagatg
     2461 atctgggact gcctccagga atacatggat tccaaagacg gcggcttcat gtcgatttcg
     2521 tacatgaagc ccatgctcct ggaaatgcct ggccgcaaca tcgccgattt cgccgatgtg
     2581 cccaaaggcg gcgggtttga atactcgggg cctatcgcgc ccatcgaaca cttcccgcag
     2641 tacgccgcca aggtggtgga actggccgcc aaatacaatg tcctgtatgc cagcacggcc
     2701 aggctcattt ccggcgggca cgccatgatg tacagcatct cgtttgcctt caaccgcaac
     2761 gacaaggcga tgatggaacg ggtggaaaag gcgctggacg aggcaacctc tttcgcgctg
     2821 ggtatgggcg gcattccctg gaagcccaat ttcaaggagc agaagatgct gcttcagaaa
     2881 atggagccca acgcgcgcaa actcctgttg atgatcaaga aaaatctcga tccgcagggg
     2941 atcatgaacc ccggcaactg ggaggtgaac gcatgaccta catccgacac attgaacatg
     3001 aaaacatcgt ccatcgctgc ttccgctgcg gttactgcaa gttcccgtcg gactacagcg
     3061 acttcaattg cccctcctac aaggccttcg gctgggatac ctactcgccg ggcggccgga
     3121 tgtggctgac ccgcgcctgg ctgtcaggcg aaatcgaaac ctccgcgcgc tttgccgaaa
     3181 tcatgttttc ctgcgtcgcc tgcgacaact gcaaggatca gtgcgtcttt ccccgattca
     3241 aggatttcct gccggacatc tttcaggaga cgcgggccga actggtcggc gaaggccgga
     3301 ttccgccggg cgtgcgcgac tatttcaagg ccgtcgcggt gagcggcaat ccctacaaac
     3361 tgccgcagtc ccagcgcggc gactgggcca aaggaacggg actgaaaccc tacaccaacc
     3421 agaaatatct tttctacgcg ggctgcgtcg gctcctacga tgaagtcggg cagaaaatgg
     3481 ccagaagcgt gggaaaaatg ctggtggatg cggggctgtc ggtcggcatt ctaggcagtg
     3541 aagagggctg tgacggcaac gacgtgcggg ctcttggcga gatgggactg tttgcccagt
     3601 tggcgcaggc caacattgaa accttcaagg ccaaaggagt cagggaaatc attacgctag
     3661 atcctcacgc gctcaatacg ttcaggaaag actaccccgt catcggcgga gtcttccagg
     3721 tccggcacta caccgaggtt ttagcccagc tgatgaaaaa gaaaaaaatc aagctcaagg
     3781 cctggccggt caaaatcacg tatcacgatc cctgctacct gggaaggcat caaaatattt
     3841 atcaggcgcc gcgggaaatt ctcaaggcga ttcccaaagc cgaatacgtt gagatgcgcc
     3901 gcagcggccc cgatgctttc tgctgcggcg gaggcggcgg caacttcttc acggacattc
     3961 tgggcacggc cgaagacagc gccgcccgcg tgcgcgtgcg tgaagctgcc gagacgggcg
     4021 cttcgattct ggcggttgcc tgccccaatt gcgccaaaat gctttccgac gcggtgaaga
     4081 tggaacaact ggaaaaccgt atcgaggtgc tgggcatcgc tgaaattctg gaacgggccc
     4141 ggaaataaaa attggaagga gccgaaatga acagagaaga aatgctcgaa gaagtttaca
     4201 aacgggcgaa ggcctacgaa ctgcgcggcg gcaactgctc gcaatgctcg cttgccgcca
     4261 tcttcgaagc gctgggcgtg gaggatgaaa acgtcgtgcg cgccgccacg ggatttgccg
     4321 acggcgtggg actatccggc gacggccact gcggcgctct ctccggcggc acgatggcca
     4381 tcagctactt tttcggccgc aaaaaggaag acttgggccg gatgggcaag cagatcaagg
     4441 cgcttcttct ggccaaaaaa ttccatgaac agtttatgaa agagttttcc acctgccggt
     4501 gccgtgatct tcaggtgaaa cagttcggcc gctttttcaa cctctacgac atggaagaca
     4561 tgaaggcggc gctggccgcg gggatgccgg aaggctgctc cacgctcgtt ggcaaggcag
     4621 cccgcatggc gcttgaaatt atcctcgacg aacaggcccg gcaggaaaaa aaggctgaac
     4681 tgcctgtggt gtaggaggat gaaattctct tcgataaaaa ttcaactgta aaggcaggta
     4741 taaactcatg attcgttcta tttccgaaag tcctctctat ccgattgtca atccaaaaag
     4801 cattgccttc ttcggcgcgt ccaacacgtt catgcgcatg ggatcgatca tgctgtcatc
     4861 gatgcaggcg ctgggatatg aagggaaaat tatcccgatt catcccaaag aaaaagttgt
     4921 ccgcggcctt gcggcatatc ccagcattct ggatgtgccg gagatccccg atctggccat
     4981 catcgtgctg cccacggaaa tcgtctgcca aacgctcgac gattgcggga agaagggcat
     5041 ccggcacgcc attgtggttt ccgggggatt ccgcgaggcg ggcccggaag gcgcggcgct
     5101 gcaaaaggaa ctggaggcga ttgcccgccg tcacggcatc cggtttttgg gccccaactg
     5161 cctgggcgtc gccaatctcc acctcaaact caatcccacg cccatcaagg cggattgcaa
     5221 ggcgggcttt gtcggcctgg cctcgcaaag cggaagcttc atcacgcaga tgttcaatta
     5281 cctccaccgc cacggcatgg gattcagcgc ggccttgagc gtgggcaacg aagccagcat
     5341 cgacatcgtg gactgcctgg aatatctggg cgcctgccct cacaccaaag tcatcaccct
     5401 gtacatcgaa gggatcaacc gcgggagcaa gttcgtggaa atcgcaaaag ccatcacccc
     5461 gcgcaaaccc atcgtcgcgc tttacgtggg cggctcagaa gcgggaaaac aggcggggct
     5521 ttcccatacc ggctccctgt ccggccccaa tgaaatttat gacgggattt tcaagcagtg
     5581 cggcatcatc cgagccaaaa cgctcaccga gctgtttgat tacgcgctgg ctctgggcac
     5641 cctgccgcgg cccgggggaa accggatcgt catccagacg cattccggcg ggcccggcgc
     5701 cacggcggcg gatgcctgca gccgcgtggg actcaagctt tcgcgcctgt cgcccgaaac
     5761 cgtgaaaaag ctcaaaccgc tgatccccaa aacggccagc acggccaacc cggtggacat
     5821 gaccttcagc aaaaatcacg cggatgatta tttcaatatt ccgaatatcc ttctttcgga
     5881 ctccgagacg gatatgctcc ttgcgtattt tgtctcgccg ggaatttttc tcgaacgcat
     5941 tctgggcgaa atgggcgttg aggaaaattc gatcaactcg gagatggaca aactgattgg
     6001 ggatttcgcg cagaagttcg taagcctcac ccaggcgcat cccgacaagc cgattgtcgg
     6061 atttacctac cgcagccttc aggaaaaaat ggttcgaact ctcctggact gcggcgttcc
     6121 cgtttatcag gatcccgagc gggcggcccg ctccctggcc gctgtgcggg agtactacga
     6181 aagacgcgga aaaatgtaaa cggcgattca accttgatga actcgtaaaa agtaattcaa
     6241 gtcgtcaccc cggcgaatac cctaaccacc tgaaggtggc gcgagggcac aagcgccgtg
     6301 gtccataact cattgtaatt actggatacc gccccccgac ttctcgaggt caggcttcgc
     6361 cggtaagaca aaaaggcgcg gaaattgact ttttacgaga ccgtcaaact tcagtttccg
     6421 ggcgaaaacg gcgaagcagg ccgccgactg cgacaggtca gcttttcttc aggcgggctt
     6481 gcaaatcgcg gcgctctttc ccctggcgcg ccagctcctc gcgattggac gcatccaccc
     6541 tcatgaagtt ctcccgccat gcatcgtccg gccattgata aggcgtttgc accgtggtgc
     6601 gcggcatcca ggcgcgttcc agaagctcaa gcgccgtccc cagaatctcc tgctgcatcg
     6661 cggcgtcccc gggttttccc gaaggattgc cgaggggaaa atcggtgaag acaaatcgcg
     6721 ccacgccgca ttcttccaca atatcccgcg cacaacccat cacaacggtg ggcagaccgt
     6781 tggcttccag gtgacgggca accagactca cggtctggtg gcaaaccggt cagagcgcca
     6841 gaagaatgac ggcatcgacg ccgtcttcac ggcaccagtt gaggatctga gggctgtacc
     6901 gctcgatggt ccttccctgg ctgtaatcgg tcggaacgcc gtaaaaccgg ggcgacgccg
     6961 atccgatgcg tccgcttgcg gcgtagctcg ccaggtgggt caacggcagg aagctgtcca
     7021 catcccgcgt atgcgtcgcc ttcttgtccc aaaacagatc gtcggtatag agcgacgcgg
     7081 gcggcggcca ggcgggctgg gcatacattt cgcggcggta cagcatggcc tccatcgggt
     7141 cggcgggctt cggcaggccc gcggtggtga ccagtccgac gcggcattgg gagagcggtt
     7201 tggctggcgg cgcaaaggga acctcgtcat aatgcggcca ggcgtaaggc ttgtcgtagc
     7261 cctgagcggc atagtaggtc cggctgcaat cgatataact cacgaaagaa cgatacggtc
     7321 tgagctgatc cataatattc ctcctcgatg gcgtgatggt attgcaaaaa gcccgcaaaa
     7381 caaatccttc ccgtgtcccg atcaatccac gattcgatcg aggtttcttt tcagtccctc
     7441 gaatccaact cttttcacgg cggagtgttg aaacagcctc ccgtattgtt cttcgttgag
     7501 gttgaaccag tcctcgcggg tcatttccag caatccaggc ggggcgtcaa agtctttttc
     7561 acggtgcgga acggcgtggc ggttccaggg gcagacatcc tggcagatat cacagccgaa
     7621 cacccgattg gccagacgat ccccgaatgc ctctccgacg gccgccttgt tctcgatcgt
     7681 ccaataggaa atgcagcggc ctgcatccag cgttcgcggg gcgacaagcg ccttcgcggg
     7741 gcaggcgtcg atgcagaacg tgcaatcgcc gcataaatcg tcaacggggt tgtcgtattg
     7801 cagggggata tcgaccagaa gcgtcccgat gaaacaaaaa gaaccttttc ccggtgaaat
     7861 caagcaggtg tttttgccga tccagcccag ccccgcccgc gcggcccacg ccttttccag
     7921 cagcggcgcc gtatccacaa acgcccggcc gtgaaccggc gcgctgccgg acaagatcca
     7981 ctccagcagc cggttgagtt tttccctgac aacggtatga taatctttcc cataagcgta
     8041 ctttgccaga acaggcgctc ggggatcggt ttgcgtcccg gacggatagt aattcaaaag
     8101 gacggaaatg accgaccggg cgccttcgaa aagccgggtc gggtcaaccc gcttgtgggc
     8161 atgtctcccc atgtaggcca tcgaggcgtg atacccgcga tccagccact tctgcaggcg
     8221 cgccgcgtct tcgtccagcg cttccgcgcg ggaaattccg cagccgtcaa atccgaaacg
     8281 gagggcttcc tccttgattg ccgccgccgt caaggccgga ttctccaggt ctttggatgg
     8341 aaaaggattt cccgcgccaa tcgctttcat ttctttcgtc ccgtttcgca agccggccac
     8401 tgcccacatc cctgcgtcaa ccacccggtc aatttccgcg aagagagcat tggacagccg
     8461 gtatcctcgc tggccgttgt gccgcgcgtc gtccgtgcgc acccctgatg catccggacg
     8521 ctgcaagacg gtcagggaac aaccgcgggc gcttccgtct gcgcaggctc gcctgcggca
     8581 gaggtctccg gcaccggcga tgccggttca ggagcttttt cttcttctcc gccttcatcc
     8641 ttcagaactt catcatattc ttcgataaca atgccgcgcc gaaccatgat ttgatatatt
     8701 ctctccgccc ggtttgccgc gtaccgcgaa ctgccgtcgg gacgaaaacg cctcggattg
     8761 ggcagggctg cggcaagctg cgccgcttca cgcgggccga ggccggcggc ggatttgccg
     8821 taatatcggc gcgcggcggt ctccacgcca aacaggccat ccccccattc ggccacgttc
     8881 aaataaagct ccagaatcct tctcttgctc aattgccttt cgattcgcca ggttagaatg
     8941 gcttccttga tttttcgcac cggatttttc gcgggtgtca aatataaatt cttggccagc
     9001 tgctgggaga tggtgctgcc tccggccttg aatttcttct tttggatgtc tttttccagc
     9061 gccttctgca tggcgtcaaa atcaaacccc tcatgggacc agaatttatc atcctcggcg
     9121 ataatcaccg ctttcatcac gtaaggcgac acccccgaca gaggaaccca ggatgcggat
     9181 atctttttct taattccttt ttccttccaa atctcttccc gatattccat gaaggcggtt
     9241 ttgccgggac gattcttctg caggccggcc acgtccgggt aaaaaaaata gcgggcgaca
     9301 tccagtatca aaaaagcgat aacagccaaa aaactccata aaattatctt tttccacttt
     9361 tgcatcaacg gcatatcctc atttttttcg tttgccgctt cctatacctc attgaaagag
     9421 attgcaaaat aaatttgaca tgccttttca tttttagtaa gagggatgcg tacgaagttg
     9481 ataaattgtt ttttaattgt aaaccttcgt tttctgtgtg cagtaaaata tctttagata
     9541 tacaaatatt agcttcaccg atccacaatc tgtgcaccgt taacaaaatg gggctccccc
     9601 cgccaaaaca ggaggtactt catgatgcaa cggcgaacca tgatttcctg gggattgttc
     9661 gctcttttct tgctgttctg ttttcaaatc gatgcccacg cggtccagta caaagttaaa
     9721 aaaggcgaaa acctttccct catttccagg cgaaccggcg tcagcgtcgc ggaaatccag
     9781 aaaacaaacc atttgaacag caacatcgta aaacccggcc agattttaag catcaaagaa
     9841 aaaaattctc catcccccgt cgccgcaaaa tcaaaaactc acccaacttc atcctatatc
     9901 gtcaaaagag gcgacacgct tcccctgatc gccaaaaaaa ccggcgtttc cgcattgcag
     9961 atcgccagac tgaatcatgt caccaccaaa tccctgcgtg ccggacagaa gttgatcatc
    10021 cccagggagg agccgcccgc gaaggcgccc gtcgaggaag aggaggattt attggaggaa
    10081 gaggatgagg gattgacgga cgacgcatcc ggccagatcg cctgtgaaga aaaagtaagc
    10141 gaggaactcc tgggcaaatg gggcagcgcc gacgaaagaa aactgttcat ccgggtggca
    10201 accggtttcc tgggcgcgcc ttaccggctg ggcggcgcca ccgtcaaagg aattgactgt
    10261 tcggcctttg tccggaaaat gtatgaattt ttcgacatca ccctgccccg caccgcccgt
    10321 gaacaatcga cggtcggcat gcaagtggga cgcgatgaac tggtggaagg agacctggtt
    10381 ttcttccgga ccaaaaaacc gatcggccac gtcggcattt acatcggcaa taacgagttt
    10441 gtccacgcct cctacaaggc aaaggccgtc cgcatcgaca gcctggatcg cccatatttt
    10501 cagaagagat ttcagcgggc cgttcgcttg aaagggctgg atgaaaacgg cggcacctga
    10561 tggcgcttct tttcacaaaa catcttaaaa aaagaggctg gaacgcctct ttttttattg
    10621 gctgttttgc cggttgctta tcggggccgt ttattataaa aggacaaccc tgccggtccc
    10681 cgcatcgcat aggcgaaaga ctgctgaatt aattgggatt atcgtaattt gcctgtaagg
    10741 aaggacccat gggaaaaaac attgtggaaa agatcctcgc cggtcatctg gtttacggcg
    10801 ccctcgcgcc cggcggggaa atcggcatcc gcatcgacca gacgctcacc caggacgcca
    10861 cgggcacctt ggcttatctg cagtttgaag ccatgaacgt cccccgggtc cgcacggagc
    10921 tctccgtcag ctacatcgac cacaatacga ttcaaatcgg ctttgaaaac gcggacgatc
    10981 accgctatct gatgagcgtc gcgcaaaagt tcggcatcgt gctgtcgcgc gccggcaacg
    11041 gcatctgcca ccaggtccac ctggaacgct tcgggcgtcc cggcaaaaca ctgatcggct
    11101 ccgacagcca cacgcccacc tgcggcgcgc tgggcatgat cgccatcggc gcgggcggac
    11161 ttgacgtcgc gcaggcgatg gcgggggagc ctttttacct cgcctgcccg tccgtggtaa
    11221 aaatcaacct ggaaggccgg ctttcgccct gggtttccgc aaaagatgtc atcctgaagg
    11281 tgctggaaat cttttcaacc aagggcaacg tcggaaccgt tttcgaatac ggaggccggg
    11341 gcctcgcgac gctgagcgtt ccggagcgcg ccaccatcgc caacatgggc gcggaatgcg
    11401 gcgtgacgac ctccgtgttt cccagcgatg atctgacccg gcaatttctg cgctcccaag
    11461 agcgggaagc ggattttatc gaactgaaag cggataccga cgcagtgtat gcccgcgttg
    11521 aaacgatcga cctgtcccgg attgagccgc tggccgccaa accgcacagc cccgacaata
    11581 ttgccacggt ccggtctctg gccggcatcc ccgttcagca ggtctgtctg ggaagctgca
    11641 ccaattcctc ctacaaggat ctggtgacgg tcgccaaaat cctcaagggc cggattgcgc
    11701 atcccgacgt cagcttcatt ctggcgcccg gctcgcggca ggtgctggac aatctcgccc
    11761 gcgacggcta cctggcctcc ctcatcgcct ccggcgcgcg gctcatggaa aacgcctgcg
    11821 gcttctgcat cggcaacagc cagagcccgc aatccggcgc ggtttcgctg cgcacctcca
    11881 accgaaattt cccgggaagg tcgggcacgc tcgacgccga catttatctg gtgagccctg
    11941 aaacagcggc ggcggcggtc atcaccggcc gcttcaccga tccgcgcgat ctggcgatgg
    12001 agtacccgga cgcgcgaacg cccgagaagc tggtcatcga cgacagtctg atcctccggc
    12061 cggacgacgc ggaccggaac gggaacatct accgcggtcc gaacatcggc gcgcctcccg
    12121 tcaacacgcc cctgcccgat acgcttgcag gtccggtgac catcaaggtc ggcgacaaga
    12181 tcacgaccga tcacatcatc cccgccggcg cgcggatgaa gtaccgctcg aatattcccc
    12241 gctacgccga attcgttttt gaaaacgtgg acgccacttt tgcccgacgt gcgctggcca
    12301 acaaagaagc gggccggcac aacatcattg tggccggcct ctcctacggc cagggctcgt
    12361 cacgcgagca cgcggctatc tgcccgatgt atctgggcgt gcgggccgtc atcgccaaat
    12421 ccctggagag aatccacgcc gccaacctga tcaacttcgg catcctgccg cttgcgttcg
    12481 ccgatgaaaa ggactacgac accctcggtg aaggcgacga gctggaaatc gcaggcctga
    12541 gaactgccgt tgccggcgga gaatccctga ccgtggccaa taaaaccaaa ggaacatcct
    12601 ttgccgtgcg cctgagcctg tcgccccggc aaaaagagat gattctggcc ggcggcgcgt
    12661 tcaacgttcg cagcccaaaa taatcccgca tcccgattcc atggagtgaa gcgcaatgac
    12721 acagaaaatc aaagtgaaga caactctcgt cgaaatggac ggagacgaaa tgacccgcgt
    12781 catgtggcgg atggtgaaag acaagctgct ctttccctat ctggacatgg acatcgacta
    12841 ctacgatctc cacgtcaagc accgcgacga cacggacgac cgggtcacgg ttgacgcagc
    12901 ccgcgccgtg cagaaatacg gcgtcggcgt caagtgcgcc acgattacgc ccaacgaaga
    12961 ccgggtgaag gaatacaacc tgaagaaagc ctggaaaagc cccaacggca cgatccgcgc
    13021 cctgcttgac ggcacggttt tccgcaaacc gattctggtg aacaacatca aaccaggcgt
    13081 gacggcctgg aagaaaccga tcacgatcgg ccgccacgcc tacggcgacg tttacgacaa
    13141 ttttgaaatg aacattcccg gcgcgggaaa gctggaagtc gtcttcacgc cggccgacgg
    13201 cggcgcgccg cggagggaac tggtgaaaaa cttcccctcg gcaggcatcg tccaggtcaa
    13261 ccacaacctg gaggcgtcga tgcggagctt tgcccgggcc tgcttcacct acgcgctttc
    13321 cgaaaagatc gacgtgtggt tttccaccaa ggacacgatt tctaaaaagt acgacgctgg
    13381 cttccgcgat gtctttgacc gcgaatttgc cgagcactgg aaagaccggt tcgacgcagc
    13441 cggcatcgag tacttcttca cgctcatcga cgacgcggtg gcgcgcatca tgaagacgga
    13501 aggcggaatc ctctgggcgc tcaagaacta cgacggcgac gtgatgtcgg acatgatcgc
    13561 gtccgcaagc ggatcgctcg ccatgatgac ctccgtcctg gtatcgccct ccggctggta
    13621 cgaatacgaa gccgcgcacg gcacggtcca gaagcattat tacaagcatt tgaaaggcga
    13681 ggaaacttct tccaattcga tggcgctgat tttcgcctgg accggcggcc tgcgcaagcg
    13741 cggcgaactg gacggcacgc cggatgtcgc cggctttgcg gacaacctgg aagcggccgc
    13801 cctggaaacg gtggaaagcg gcgtgatgac gggcgatctg ctgctcctgg ccgaaaagcg
    13861 gcccaccaac cggaaggtca acacggaagg ctttatcgac gcgatcgccc aaaacctgaa
    13921 gaaaaaactt tagaacggga aacacgcacg cccgagggtg cgcactaaac cacaggatgt
    13981 agtgcgggtc ttcagacccg cttttcaaaa caaggattgc aaccctttgt tcccttcgca
    14041 caaaaaccag cgcagctaaa gctacgcact acagaaaaac gtgcatgaaa acggacatga
    14101 aaaacgtagt gcgggtcgct tgtgaccttc aggttatcag acccgctttc cagaatgcaa
    14161 gaatccggaa ttcatcatga gtaaacgcaa cgaaaattcc ttgcccaatc tgtcggactt
    14221 cacccttccg gagctggagg aattcatcgc cgcctccggc aaggaaaaat accgcgcccg
    14281 ccagatcatg aaggagatcc accagttcgg ctgcctctcc ttcgacgcca tgacgacgct
    14341 tgcgcgcgat ttccgcaaaa caatcgccca aacggcccga atctaccgcc cgtcgatggt
    14401 caaaatccag aagtcacagg atggcacaca aaaagttctg ctgggcctcg aagacggtct
    14461 ttttgtcgaa agcgtgctga tccccggcaa aaaccactgg acactgtgca tttccacgca
    14521 ggccggctgc gcgatgggct gcgccttttg tctgacggcg cgccagggtt tcaagcgcaa
    14581 tctcacgccg gcggaaatcg tcggacaggt ggtaaccata aggaaagaaa cacccgaggg
    14641 agaggccgtg ggcaacattg ttctgatggg catgggcgag ccgctcgcca actacgaaaa
    14701 tacggtgaaa gcgattcgca tcctcaccag cgatttcggc ctgggctttt cttcgcggcg
    14761 catcaccgtt tccacctgcg gactcgcccg gcagatcctg caactgggaa gcgacatctg
    14821 catcaacctg gccgtatcgc tcaacgccca cgatgacgaa acccgcagtc ggctcatgcc
    14881 ggtcaacaaa aaatatccgc tggcaacgct tctggacgcc tgcaaaaaat atcccatgcc
    14941 gggccgccgg atgctcacgt atgaatatat tctcatcgac ggtgtgaatg cctcgcggca
    15001 agatgcccaa aagctgaccc tgctccttgc ggatcagcgc tgcaagatca acctgatcgt
    15061 cttcaacgaa taccccggct cgccctttcg cgcgccctcc ccccacgtcg tgtcggattt
    15121 tcagcaggtg ctgctcgacc ggcacttcac ggccatgctg cgcaaaagca agggctccga
    15181 catcctggcc gcctgcggac agttgagcgg aacttacacg accggttaac ctggcaaaca
    15241 aagaattatt tctcgcccgt aaaaaggaag gtcttatcaa ttcatttgac tttagtatcg
    15301 tattccagta ccattattca caatgaattc taaacataga aaaacgcttt ctgaaatatt
    15361 cagaaatccc gttccatcaa atatcccctg gatggatgta gaaagtcttc tcatttcgct
    15421 gggcgctgat atttcagaag gaagcggatc tcgtgttcgg attcaattga atgatgaaag
    15481 agctgttttt catcggccgc acccgcgaaa agaaacaaac aggggcaccg taatttccat
    15541 gagacgattt ttggaaaatg caggagtaaa acatgatgga gtataaaggg tacataggaa
    15601 ttgtagcgta tgacgatcaa gccaggattt ttcacggtga tgtaattaac acaagagatg
    15661 ttattacatt tcagggaaag tctgttcgtg aaattgaaaa ggcattcaaa gaatccatag
    15721 acgactatct atcatggtgc gcaaaagatg gcgtagcccc tgaaaaacca tattcaggaa
    15781 aattcaattt acggctgtcc cccgatttgc atcgtgaagt tgcggtaacg gcaaagaaac
    15841 taaatatttc tattaactct tttgtcgaac gcgccttgat tgatgaaatc aatacacaca
    15901 gagcaacata aatagcaaca ggatagatgg tcgaatgatt ttttacggag aaaaccatga
    15961 ggaaacgact tttaaaaaaa ataattcatg agggtgatta tatcgcagaa gttgatgttg
    16021 aattgattga cactgatgaa ggatggtccc catatctttc tttggaagat gcacaagaat
    16081 tagacgattt gcgtgattat ctgagacgag gtgatcttca ctccgcggcc cgattagccc
    16141 gtctgtattc cttgacggct attgcagtct agaaaattat ggcgggcgct tcggaagtcc
    16201 cttacagaag ggagcggatt tcagaagctg ttcaagaaag atgccgatat tgccaaggac
    16261 cttacggaga atgaacttgc cgcgatcttc aatgtcaaca atctcctgaa gaatctggat
    16321 tttatttttc accgggtgct tgaagagaag gaacaaagct gcttagtttt tctttcacag
    16381 gagtttcggg gataaaccaa ttcgtgcgac gattttggag taataacggc ag
//
LOCUS       DAOSRM010000045        16230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3691_length_16230_cov_106.989861, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000045 DAOSRM010000000
VERSION     DAOSRM010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 16230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16230
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3691_length_16230_cov_106.989861"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1439
                     /gene="mnmE"
                     /locus_tag="PLA58_05925"
     CDS             36..1439
                     /gene="mnmE"
                     /locus_tag="PLA58_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOR62369.1"
                     /translation="MNSKNVENMMETDTIAAIATPLGTAGVGLIRVSGPLAPELAERI
                     FRPAHPNCAWQSHHLYHGDIVSPDGKSVLDEVLATLMRKPHSFTGEDVLEISCHGNPL
                     ILQTILEQLLELGCRPARPGEFSERAFLHGRMDLSQAEALAALISAQSAKALQIELSQ
                     MKGSLTRRIDELRSLLIDALASMEAAIDFAEDVPEKEIPAVSGQMLRAAAGMDQLLST
                     YRQARLFTEGLRVVIAGKPNVGKSSLLNRLAGRKRAIVTDIPGTTRDLITATLDLDGT
                     CVRLTDTAGIRPPTDAIEKEGIDLVWQQMEETDILLLLFDGSKPLTDEDIQILEQSQT
                     YDVKILMAVNKCDLPPAWSNLLPDHPLYGKSLLRLSAKFGDGLEDLKKAITGLTDPGE
                     APAEGVLITRVRHKLALEAACNSLRAAQTCLTTGQSPEFAAFELAEALNALDEITGKK
                     ISGDVLARVFSTFCIGK"
     gene            1542..2672
                     /locus_tag="PLA58_05930"
     CDS             1542..2672
                     /locus_tag="PLA58_05930"
                     /inference="COORDINATES: protein motif:HMM:NF027862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HOR62370.1"
                     /translation="MKIASCLLSVLCLLCFSVPDAFAQAKCIPAGGEALIVNNDKPSA
                     KMEAVARAKWAAIEDVAGTEVKAGSIVQNFTLVEDVMKTKAGGVVKSYQVLDEFVKDD
                     ILNVKINACVVPSQARDAVSQLGLNNSIALFIPARKPGRHGDEFEENNILSETLIGKL
                     TEQGYTVVDVAPTHAVDAREIEKAVRSGSTLSLRSMMYKFLSNLIIIGKVDYTISTQK
                     GEDIGYGLAMPFNHVTVRLNYRILSKNNKTGGMEILTTGVEQAKGLANNVEDATAEAM
                     KELAKDLTPKLLDKVGQYIQGNTKKIAVQVKGVNDLDTALEVKGILQNIVWVAGVEEK
                     EMGSFVVSYPENTLYLANSIQQKGSFKIVNFSPYSLTVEYLK"
     gene            2688..3761
                     /locus_tag="PLA58_05935"
     CDS             2688..3761
                     /locus_tag="PLA58_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOR62371.1"
                     /translation="MMFNTRGKYGVFHVMVLAILPITLLMLSGCATKIRVNMLQPAQY
                     HEASLTKAVAVLPFNGPGGTEFAAEIEGVLAGIGIDDKPYFTLVDRASIDKIISEMKF
                     SQSGLVDPKTAVKLGKMIGAQGIYTGVVTQNSCSDSRYAEKRQTCTQYEMRRDEKGRT
                     YQGPCLRWRAYHVQCTKRVANFAVSPKLVDVSTGRVIYSRNLSAVTNSSGCEDANPVQ
                     SETVLLEQAKASVKNQFRRDIAPYYITREIRLIDSTDGIDSKEAKDLLKRGLEFAGHD
                     RMDSACELWGQARNLAPGSYAILYNLGVCAESRGDLDAALNLYRQSDQILGKPDDDIS
                     LALTRVGEAIKNRGKIKEALGQK"
     gene            complement(3763..4773)
                     /gene="cydB"
                     /locus_tag="PLA58_05940"
     CDS             complement(3763..4773)
                     /gene="cydB"
                     /locus_tag="PLA58_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOR62372.1"
                     /translation="MENIQNFQILWFVLIFVLLLGYTLLDGFDLGLAALLPFLGKTKE
                     EKDTLIASIGPVWDGNEVWLITGGGALFAAFPHAYATAFSGFYLAMMLVLLALIFRAV
                     SMEFRANDPVRSGLWEKALVVGSALAALLFGVALGNIIYGVPLDDKMEYAGGFFSLLR
                     PVPLLFGLTGLAAVLLQGASWAVLKTEGSLQDRSFQAVRALTAVNAVLAAVYFTVLFV
                     VVLRISGNPFFYAAVLLTLAGLLTLFLAPGKKHETGPIRGSSLSLTGLFLAAGAAQFP
                     NLITASNDPGLSLTIYNSSTGIYTLQVMAVIALAGMPVVIGYTIFVYRLFRGKAKADD
                     HY"
     gene            complement(4800..>5676)
                     /locus_tag="PLA58_05945"
     CDS             complement(4800..>5676)
                     /locus_tag="PLA58_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOR62373.1"
                     /translation="ANSWMQTPAGYEITSAGKIVLTDFGAAVFNPSTVARFLHTVLAT
                     WVTGAVLVCGIAGYYVRKGLHGETARMMLKIGIILFAVTPLLQLGAAHGHAIQVIDFQ
                     PMKAAAMEGHFTTARGADVYVAGYVDEKNEKTYGISIPKGLSFLYNFDFNSEIKGLRE
                     VPKEDWPPVNLVFQAYHIMVTLGMIFIGLGLLGALLLWTGKLYTAKWYLWLLPFLIPL
                     PHLAHETGWITAEVGRQPWIIQGLMRTARASSVVVSKGELAASLTMFALVYLLLLVMF
                     VMLFIRIVKQGPEAS"
     assembly_gap    5677..5776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5777..5985)
                     /locus_tag="PLA58_05950"
     CDS             complement(<5777..5985)
                     /locus_tag="PLA58_05950"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOR62374.1"
                     /translation="MDALLLARLQFAFTIGFHFLFPAMTLGVALIILISETLHLATKD
                     DVYRRITDLLARLLGLIFVIGAATG"
     gene            6116..6478
                     /locus_tag="PLA58_05955"
     CDS             6116..6478
                     /locus_tag="PLA58_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62375.1"
                     /translation="MMKKHLWNVALMVVWLAAALLLPQQAAADPPRDLSLRYDMESQT
                     LSVSITHKSTFTDFHYIKQVQIIRNNEPFEIQKYSSQPGKTTFVYTHKVPAKPNDLLE
                     VTAACNVQGQKTATLKVE"
     gene            6493..8337
                     /locus_tag="PLA58_05960"
     CDS             6493..8337
                     /locus_tag="PLA58_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HOR62376.1"
                     /translation="MAAKATLKKPIRIMTLSNPATAASPPTVRTFCCALLLFCLFSGS
                     GLVAVAAAADPIEVVVEGIDGDARKNVLEALALPPGLVQNGKADRLWLERFARQAERK
                     VPAALEPFGYYSAKTVIQIETPEADKYRLRVQIDPGQPAVITGVNVTVTGSGASEATL
                     AALASDFPLRKGAVLVHPLYEKAKQALQSRAEELGYLDAAYAVHEVRVDEAGRSAVIT
                     LALETGQKYFFSTVAIKGAPDYPDAFLRRYLTFQPGEVFSYSRMAASQLQLNNSERFR
                     RVIVRADKEGARDGKIPVQVILTPAPHISLRPGIGYGTDTGARFALHYRDLNMLHRGH
                     ELDSRLYISQRLQGIATSYILPSDRDIRTFTSAQINLQQEEISTYDSRIISMELARTH
                     SFASRGQGSVYVKFQREEFTIGSQDSTSRYILPGVRFSDSRFNNLIRPDRGFRYAFDL
                     RGAHQALGSTTRLLQFIGEAGHILPLPWRLSFLASAKAGFTVFTDEFSDLPPSLRFFA
                     GGDQSVRGYAYQSLGPRDAQGNVVGGKHLLIGGVELQRAILENWAVSVFYNAGNAFDT
                     FSDLTLFQAAGAGIHYHTPVGALNLYIARQIRVDDPEFRIHFTVGVEF"
     gene            8334..12311
                     /locus_tag="PLA58_05965"
     CDS             8334..12311
                     /locus_tag="PLA58_05965"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOR62377.1"
                     /translation="MKRALKYLILIAAFGAPAVILAGAGWLACSEAGSRKLLEAVSRF
                     TPVKIKAETIEGNLADTLRLTNTIIEWPQGNIRIRQLKISIRPLEAPAGHLGISLLSA
                     DQVSVLDNSPKERPELVWPQAKGWITFVSGGIGRLAIRDLTYLRPDEQPVRIASLGAS
                     VGWKNSRLSISDLRINSSRGLLEGNILAGFHRPLLKANLAATLTRPAAGVDFIRLDGA
                     FKPGRRPGEMNGSLQVDGLRNTRPILRMSSDLGMTPAGLSFSKIRLRRENRPGSVAGE
                     GLLTFKGPTPCITMRIQAENVDLSDELKIPANLSGSLLFEGTGRQYQGRVTLANTADT
                     RHAFRLAGDYSGDSESATLSRIQGTALGGSLNGRLEIRWTDGLRLNGAMSGKNLDPAR
                     WDPEWAGVIHFDLAGDVSAKAGEPASGALNLTLKESRLHGRQLTGDLRASFAGDDIDI
                     HHLALRGKGFEISARGSIADQLDFKAEIRDLARLIPRASGALTASGWARRRHDRFSGV
                     VSARGHSLSADGWSIASANFSAFLDDREKSPFSLTAAFSRLRFQTLTADTLTLRGGGT
                     VLSHTLDAFTRRGPHEMHLGLTGAWRRKGWQGKIVRLEGRDGVGPWRLSAPAALAVSR
                     ESLALGPMEITGRGPEALRLSAALSGNPLTGPVDLRWNSLDLARANAWIDRDILAGVC
                     SGSLQATLLPFNRIDLAGETTLTGTLQTQGQTVEIRQGKMTLNADRRGVRLDMKIEPS
                     EGGALSGAFVSSGPAERSIPETGEWNLRWRELDLKRFSAALPRRTRLEGKISGEMNGT
                     LLPDRRFSLSGRAGLDGSKINRRGQRGDVSIDLRRASIHWLWQKDYLECELHVTMADL
                     GALQGKIRLPLAAHFPLVMNEQGNLQGSLMGQVQEKGALGVLFPGLVQESRGNLDLDM
                     KMRGSPADPLFEGRVRLSKAGGYLPSAGITLQDVRIDARLAKDGVTIDSFRAVSGPGY
                     LDGTARIRLKGFKIADFEGNLNGDRFQAIHVPELHVQTSPRLTFSGTPEKISVRGEVL
                     LPLLRVNGSQSQGPVESSPDLVREGKTKSGTGKPSVGLDVQVKLVLGEQAEFNAAGID
                     AKLGGEMEIRFSDPERISGRGEIRVVKGRFRTYGVNLEIVRGHLFYAGGPVNQPALDI
                     LAIRKIGEVKAGVAVSGKLPKPLVKLYSEPYMQDMDILAYIVLGHPLGADSKQAGLLA
                     TAAGAVLTSQQAEDLLEQIQDYLGLASLEISSDVLEKSNQMGYKRLKVTPEGTGSSSP
                     GASETILVVGKYLTPELYISYGRSLFSGGNLFFLRYDLSKNWQVESQMGQESGIDLYY
                     KLEFH"
     gene            12326..13675
                     /locus_tag="PLA58_05970"
     CDS             12326..13675
                     /locus_tag="PLA58_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOR62378.1"
                     /translation="MPSRHFDLAIAGGTLVTMSSRMDIIENASIGISGGRIALVDTNR
                     TLAADRIIDARGGLILPGLVNTHTHLPMVCFRGLADDLPLMDWLHHHIFPMEARFVNK
                     QMVRDGAMLAIAEMILSGTTTCCDGYFFENQIAEAALAGGMRAVAAQGFADSATPDHP
                     QYEKMMAAAGRFVERWLPHAPMITPAFFCHSPYTCSPRTLVNVKAAAREAGILYLTHL
                     LENRDEIDTIEKRYGRKPVQHLHHLDVLDDRTVAVHCNWMSPEDIALFADLGVSVSHN
                     PASSMKLAAGVAPVPLMLEKGIAVGLGTDGSASNNDLDLFQEMDLSAKVHKATLLDPT
                     VMSAETVLKMATIEGARVLGMDRLIGSVEVGKQADIIILDMNQPHLTPLYNPYSQIVY
                     AARGGDVKTSIIDGKPVMENRRLLTMDLPAVMERVRAIAAHIQSEHQSAIRTSPVDV"
     gene            complement(13724..14728)
                     /gene="msrB"
                     /locus_tag="PLA58_05975"
     CDS             complement(13724..14728)
                     /gene="msrB"
                     /locus_tag="PLA58_05975"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOR62379.1"
                     /translation="MIQTENAAVATLAGGCFWCVASDMKKLPGVIRVVSGYAGGSEKN
                     PTYETYMAQGHLEAAQVHYDPKVVTYEQILTYFLKHIDPTDAGGQFADRGPGYRSAVF
                     YNDEAERKTAAGALQALAASGKFGKPIVTELKPFTGFYPAEDYHQDYDEKNPLRYRAY
                     RSGSGREGFLREVWKKDQSNTPASTPLYRKPDDQTLKKTLSPMQYNVTQKSATEPPFQ
                     NEYVHNKQEGIYVDVVSGEPLFSSLDKYDSGTGWPSFTRPLEPANILTKEDRSFFEAR
                     TEVRSRHADSHLGHVFPDGPGPAGLRYCMNSAALRFVSKEDLEKEGYGRYLKLFSPGK
                     "
     gene            complement(15005..15289)
                     /locus_tag="PLA58_05980"
     CDS             complement(15005..15289)
                     /locus_tag="PLA58_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62380.1"
                     /translation="MKKVLILACMFLLVATVALAAGPKTYQVTGPVLDVKGDVIVVKK
                     GSDNWEVAKSAATKVVGDVKVGSKVTIEYTMTATKIEAKEEKKAAEKKKK"
     gene            complement(15327..15650)
                     /locus_tag="PLA58_05985"
     CDS             complement(15327..15650)
                     /locus_tag="PLA58_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOR62381.1"
                     /translation="MLKKKHFLCLRNGCLLFAALILCAALIAAAASALAAPKEAAEKL
                     APAVRVNINKADQATLEKLLGIGPVKAKAIIDGRPYRNIEDVMNIKGIKGKTYDAIKG
                     RIIVE"
     gene            complement(15858..>16230)
                     /locus_tag="PLA58_05990"
     CDS             complement(15858..>16230)
                     /locus_tag="PLA58_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62382.1"
                     /translation="RLRLKQLSLLIFRFAKIYRTKRSQWPLKEIFGQQDRFRPDLLSG
                     GAGAKRFLENYVNGRMRFEFLIQIEPVGFALHRQFNMETNIFGCCTLTRQGDPAGTIA
                     DNFMNRALHIIRIPADDFIGP"
BASE COUNT         3616 a         4720 c         4497 g         3297 t
ORIGIN      
        1 ggcgataggc gataggcgat aggcgatagg cggtggtgaa cagtaaaaat gtggaaaaca
       61 tgatggagac agacaccatt gcggccatcg ccacgccttt gggaacggcg ggtgtgggtc
      121 tcatccgggt cagcggaccc ctggcccctg agctggcgga gcgaattttc cgtcccgctc
      181 accccaactg cgcctggcaa agccatcatt tgtatcacgg agacattgtc tcgcccgacg
      241 gcaaatcggt tttggacgag gtgctggcca cgctgatgcg caaaccgcat tctttcacgg
      301 gcgaagacgt tctggaaata tcctgccacg gcaatcccct gattttgcaa accattctgg
      361 agcagctcct ggaactgggc tgccgtcctg cccgccccgg tgaattttcc gagcgcgctt
      421 ttcttcacgg ccggatggat ttgtcgcagg cggaagcgct tgccgctttg atttccgcgc
      481 aatcggcaaa ggcattgcag attgaactgt cgcagatgaa aggctcgctg acgcgcagaa
      541 tcgatgaact gcgctcgctg ctgatcgacg ctctcgcgag catggaagcc gccattgact
      601 ttgccgagga tgtccccgaa aaagaaatcc ccgccgtctc cgggcaaatg cttcgggccg
      661 ccgccggcat ggaccagctc ctgtcaacct accggcaggc ccggttattt actgagggcc
      721 tccgcgtggt cattgccggc aagcccaacg tgggcaaatc cagcctgctc aaccggctgg
      781 ccggccggaa aagagccatc gtaacggaca tccccggcac cacgcgggac ctgattaccg
      841 caacacttga tctggacggc acctgcgtcc gcctgacgga caccgccggc attcgcccgc
      901 ccacggatgc tattgagaaa gaaggcatcg acctggtctg gcagcaaatg gaagagaccg
      961 atattcttct gctgctgttc gacggcagca aaccgctgac ggacgaagat atccagatcc
     1021 tggaacaaag ccaaacctac gacgtcaaaa tcctgatggc cgtcaataaa tgcgacctgc
     1081 cgcctgcctg gtcgaacctg cttcccgatc atccccttta cggcaaatcc ctcttgcgcc
     1141 tgtcggcaaa attcggcgac ggtttggagg acctcaaaaa agccatcacc ggattaacag
     1201 acccgggcga agccccggcc gagggcgtcc tgattacccg ggtgcgccac aaactggcgc
     1261 tggaggcggc ctgcaacagc ctccgggcgg cccaaacctg cctcaccacc ggccagtctc
     1321 cggaatttgc cgcctttgaa ctggccgaag ccctgaatgc gctggacgaa atcaccggca
     1381 aaaaaatttc aggcgatgtt ctggcccggg ttttctccac tttctgcatc ggcaaataaa
     1441 ttgaaaaccg cccctcttta tgatagtttt cacaaggttt caaagatttg ctgcagagtc
     1501 gttttgctga cccttaccaa atatttttaa gaggtggctt catgaaaata gcttcttgtc
     1561 ttctttccgt gctttgcctg ctttgttttt ccgtccctga tgcattcgcc caggccaaat
     1621 gcattcctgc cggcggcgaa gcgctgatcg tcaacaatga caaaccctcg gccaaaatgg
     1681 aagccgtggc ccgcgccaaa tgggcggcaa tcgaagacgt cgccggaacg gaagtcaaag
     1741 ccggcagtat tgtgcagaac ttcacgctgg ttgaagatgt gatgaaaacg aaggcgggcg
     1801 gcgtggtgaa aagctatcag gtgctcgatg aattcgtgaa ggacgacatt ctgaatgtga
     1861 aaatcaacgc ctgcgtcgtg ccgtcgcagg cccgcgacgc cgtttcgcaa ctgggcctga
     1921 acaattccat tgctcttttc attcccgccc ggaaaccggg acgccacgga gatgagtttg
     1981 aagaaaacaa catcctttcc gaaaccctca tcggcaaact gaccgagcag ggctacaccg
     2041 tggtggacgt cgctcccacc cacgcggtgg acgccagaga aattgaaaag gccgtgcgct
     2101 ccggcagcac gctgtccctg cgcagcatga tgtacaaatt tctgtcgaac ctgatcatca
     2161 tcggcaaggt ggactacacc atttccactc aaaaaggcga agacatcggc tacggccttg
     2221 ccatgccctt caaccacgtc accgttcgtc tcaattaccg gattctttcc aaaaacaaca
     2281 aaaccggcgg catggaaatt ctgacgacgg gcgtcgagca ggccaaagga ctcgccaaca
     2341 atgtcgagga tgccacggcg gaggccatga aagagctggc gaaggatttg acgccgaagc
     2401 tgctggacaa agtcgggcaa tacattcagg gcaacaccaa aaagattgcc gtgcaggtaa
     2461 aaggcgtgaa tgatctggat acggcgctgg aagtcaaagg cattcttcaa aacatcgtct
     2521 gggtcgctgg ggtggaagaa aaggaaatgg ggtcgtttgt tgtcagctat ccggaaaaca
     2581 ctttgtatct ggccaacagc attcagcaaa agggaagctt caaaattgtc aatttctccc
     2641 cttattccct gacagtggag tatctgaaat agtcaaataa ggaggcgatg atgttcaata
     2701 cgcgcggaaa gtacggtgtt tttcatgtta tggtcttggc gattctgccg atcaccctcc
     2761 tgatgctgtc gggctgcgcg acgaaaatcc gcgtcaacat gctccagccc gcgcaatacc
     2821 atgaagcatc gctcaccaaa gccgtcgccg tactgccgtt taacgggccc ggcggaacgg
     2881 aatttgccgc tgaaattgaa ggcgtactcg cgggcatcgg catcgacgac aaaccgtatt
     2941 tcacgctcgt tgaccgcgcc tccatcgata aaatcatcag tgaaatgaaa ttttcacaaa
     3001 gcggtctggt ggatccgaaa accgccgtga agctgggcaa aatgatcggc gctcagggaa
     3061 tttacacggg cgttgtcaca caaaacagct gcagcgacag ccgttacgcg gaaaaaaggc
     3121 aaacctgcac acaatatgaa atgcgccggg acgaaaaagg aagaacctat cagggcccct
     3181 gtttgcgatg gcgcgcctat catgttcagt gcacaaagcg cgtagccaat tttgccgttt
     3241 ccccgaagct ggtggacgtc tccaccggcc gggtgatcta ttcccgcaat ctctccgccg
     3301 tgaccaattc ttcaggctgt gaggacgcca atccggtcca gagcgaaacc gttttgctgg
     3361 agcaggccaa agcttccgtc aaaaaccagt tcagacgcga tatcgctccc tattacatca
     3421 cccgggaaat ccgcctgatc gattccacgg acggcatcga ttcgaaggaa gcaaaagatc
     3481 tcctcaagcg aggcctcgaa ttcgcggggc acgaccgcat ggacagcgcc tgcgaactct
     3541 ggggacaggc ccgcaacctc gctcccggct cctacgcgat tttgtacaac ctgggcgtct
     3601 gcgcggaaag ccggggcgac ctggacgccg cgctgaatct ttaccggcag tccgaccaaa
     3661 tcctgggaaa acccgatgac gatatttcgc tggcgctaac ccgcgtcggc gaagccatta
     3721 aaaaccgcgg caaaatcaag gaagccctgg gtcagaagta aatcaataat gatcgtcggc
     3781 tttggctttt cccctgaaga ggcggtaaac aaaaatggtg tagccgatca caaccggcat
     3841 gccggccagg gcgatgaccg ccatcacctg cagcgtgtaa atcccggtgg agctgttgta
     3901 gatggtcagg ctcagccccg gatcattgga ggcggtgatc agattgggga actgggcggc
     3961 gcccgcggcc agaaacaatc cggtcaggga taaggacgac ccccggatcg ggcctgtttc
     4021 gtgtttcttc cccggcgcaa ggaacagcgt cagcaatccc gcaagcgtca gcaaaaccgc
     4081 cgcgtaaaaa aacgggttcc ccgatatccg caaaaccaca acaaacaaaa ccgtgaaata
     4141 aacggccgcc aggacggcat tgaccgccgt cagcgcccgc acggcctgaa atgaacgatc
     4201 ctgcaggctc ccttccgttt tcagcacggc ccaggacgcg ccctgcagca ggaccgcggc
     4261 aagccccgtc aggccaaaca aaagcggaac gggacggagc agcgaaaaaa aaccgcccgc
     4321 gtattccatt ttgtcatcca gcggcacacc gtaaatgata ttgcccagcg caaccccgaa
     4381 cagcagggcc gccagagcgc ttccgacgac cagagccttt tcccagaggc ctgaccgcac
     4441 cggatcgttt gcgcggaatt ccatggaaac cgcccggaaa atgagggcca gcaaaaccag
     4501 catcatcgcc agataaaaac cggaaaacgc cgtggcatac gcgtggggaa acgcggcaaa
     4561 aagcgcgccg ccgccggtga tcagccagac ctcgtttcca tcccacaccg ggccgatgga
     4621 agcaatgagc gtgtcctttt cttcttttgt tttgcccaga aacggcagca gtgcggccag
     4681 cccgagatcg aatccgtcca aaagcgtgta tccgagcagc aggacaaaaa tcaggacaaa
     4741 ccaaaggatt tgaaagtttt gaatattttc catcgcagtc gatccccctt acagcacatt
     4801 taagaagcct ccggcccctg tttgacgatt cttataaaga gcatgacaaa catgaccagc
     4861 aacagtaaat acaccagggc aaacatggtc aggctcgcag ccagttctcc ttttgatacc
     4921 accacggacg aggcccgggc ggttcgcatc aatccctgga tgatccaggg ctggcggccc
     4981 acttccgccg tgatccaccc cgtttcgtgg gccaggtgcg gcaggggaat caggaaaggc
     5041 aaaagccaga gataccattt cgcggtgtag agttttcccg tccacaaaag caaggccccc
     5101 agaagcccaa ggccgataaa aatcatgccc agcgtcacca tgatgtgata ggcctgaaac
     5161 accagattca ccggcggcca gtcttctttg ggcacttccc gcagaccctt gatttccgag
     5221 ttgaaatcga agttgtacaa aaagctcaaa cctttgggga tggaaatccc gtaggttttt
     5281 tcatttttct cgtccacgta cccggcaacg taaacatccg cgccgcgggc ggtggtaaaa
     5341 tgtccctcca tcgccgcggc cttcatcggc tgaaagtcga tgacctggat cgcatggccg
     5401 tgggccgcgc ccaactgcag aagcggcgtg accgcgaaaa gaataatgcc gattttgagc
     5461 atcatccggg ccgtctcccc atgcaggcct ttccggacgt agtagccggc gattccgcaa
     5521 accaggacgg cgccggtgac ccaggtggcc aaaacggtgt gcaaaaagcg ggcaacggtg
     5581 gacgggttga aaacggcggc gccaaagtcc gtcaaaacga ttttaccggc ggaggtgatt
     5641 tcatatccgg cgggcgtctg catccaggag ttggccnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnatgc cggtggccgc gccgatcaca aagatcagcc ccagcaatct
     5821 ggcgagcaga tccgttattc tccggtaaac gtcatctttc gtagccagat ggagcgtttc
     5881 ggaaatcaaa atgatcagag cgacgcccag cgtcatggcc ggaaagagaa aatggaatcc
     5941 gatggtaaag gcgaattgca ggcgggcaag gagtaacgcg tccatagctt cctccgtgat
     6001 tgtagtggaa agcagaatat agaattgttc cgcttttttc aatctctaat ttcctgttgt
     6061 tatttcctcg gcaattgtat tactgtaggt caaccatatc ctttcaggag aatatatgat
     6121 gaaaaaacat ctttggaacg ttgcgctgat ggttgtctgg ctggccgccg ctttgctcct
     6181 tccgcagcag gccgccgccg atccgccccg ggacctttcg ctgcgttatg acatggaatc
     6241 ccagacactt tcggtgtcca tcacgcacaa atccacattc accgatttcc actacatcaa
     6301 gcaggttcag atcataagaa acaatgagcc gttcgaaatc cagaaatact cgtcgcaacc
     6361 cggcaaaacc acttttgtct atacgcacaa agtgccggcg aagccaaacg atctgctgga
     6421 ggtgacggct gcctgcaatg ttcagggtca gaaaacggcg acgctgaaag tggaataaaa
     6481 aaagaaaagg cggtggccgc aaaggccacc ttaaaaaaac cgatcaggat catgaccctt
     6541 tccaatccgg caaccgcggc atcgcctcca accgtgcgaa cattttgctg cgctctcctt
     6601 cttttctgcc tgttttccgg atccgggctt gttgccgtcg cggcggcagc cgacccgata
     6661 gaagtcgtcg tggaaggcat tgacggagac gcgcgaaaaa atgttctgga ggcgctggcc
     6721 ctgcctcccg gcctggtgca gaacggaaaa gcggaccggc tctggctgga gcggtttgcc
     6781 cgtcaggccg aacggaaagt accggcggct cttgaaccct tcgggtatta ttccgccaag
     6841 accgtcatac aaatcgaaac ccccgaagcg gacaaatatc ggctgcgggt gcagatcgat
     6901 ccgggtcagc cggccgtcat caccggagtc aacgtcaccg tcaccggcag cggcgcgtcg
     6961 gaggcaacgc tggcggcgct tgcttccgac ttccctctgc gaaaaggcgc cgtgctggtg
     7021 catcccctct acgaaaaagc caaacaggcg ctgcaatcca gagccgaaga gctggggtat
     7081 ctggatgccg cctatgccgt tcacgaagtc cgggttgacg aggcaggacg gtccgccgtc
     7141 atcacactgg cgctggaaac cggccaaaaa tattttttct ccacggtggc catcaagggg
     7201 gctcccgatt atcccgacgc ctttctgcgg cgttatctca cctttcagcc cggagaggtt
     7261 ttctcgtatt cccgaatggc cgcctcccag cttcagttga acaattcgga acggtttcgc
     7321 agggtaatcg tccgggccga caaagagggg gcccgggacg gcaaaatacc ggtacaggtg
     7381 attttaacac ccgcgccgca tatcagtctg cggccgggca tcggctacgg aacggataca
     7441 ggagcaagat tcgccctgca ctaccgcgat ctcaacatgc ttcaccgggg acacgaactg
     7501 gattcgcgcc tgtatatctc tcagcgactg cagggaatcg cgacgagcta tattctgccg
     7561 agcgacagag atattcggac attcacctcc gcgcagatca acctgcagca ggaggagatc
     7621 agcacctatg acagccggat catttcgatg gagcttgccc gaacgcacag cttcgcttcc
     7681 aggggacaag gttcggttta cgtgaaattt cagcgcgaag aattcaccat cggcagccag
     7741 gactcgacct cgagatatat tcttccgggc gtccggtttt ccgacagtcg cttcaacaac
     7801 ctgattcgcc ccgaccgcgg gttccgctat gccttcgatc tgcgcggcgc gcatcaggcc
     7861 ctgggctcca caacccgcct gcttcagttc atcggcgaag ccggtcatat tcttcccctg
     7921 ccgtggcgtc tgtcttttct ggccagcgcc aaagcgggct tcacggtttt taccgatgag
     7981 ttttccgacc tccccccgtc tttgcggttt ttcgcgggcg gcgatcagag cgtccgcggc
     8041 tatgcctatc agtcgctggg cccccgagac gcgcagggca atgtggtcgg cggcaagcac
     8101 cttttaatcg gcggcgttga attgcagcgc gccatcctgg agaactgggc ggtatccgta
     8161 ttctacaacg ccggaaacgc tttcgacact ttttccgatc tgacgctgtt tcaagctgcg
     8221 ggcgcaggca tccattacca cacgccggtc ggcgccctga atttatacat cgcccggcag
     8281 attcgcgtgg acgatccgga attccgcatt cacttcaccg tgggggttga attttgaaac
     8341 gtgcattgaa atatcttatc ctcatcgccg ctttcggcgc gccggcggtt attctcgcag
     8401 gcgcgggatg gcttgcctgc agcgaagcgg gaagcagaaa actgctggaa gccgtttccc
     8461 gcttcacgcc ggtgaaaatc aaggcggaaa ccatcgaggg aaatctggcg gacacgctgc
     8521 gcctgacaaa tacgatcatc gagtggccgc agggaaacat ccggatacgt caattgaaga
     8581 tttccatacg gccgctggag gcgccggccg gccacctggg gatttccctt ctttccgcag
     8641 accaggtatc ggtattggac aactcaccga aagagcggcc ggagctggtc tggccgcagg
     8701 ccaaaggctg gattaccttt gtgagcggcg gcatcggacg cctggccatc cgtgatctga
     8761 cctatctccg ccccgatgaa caaccggttc ggatcgcctc tctcggcgcg tccgtcggct
     8821 ggaagaattc acgcctgtcc ataagcgatc ttcgcatcaa ttcatcccgc ggccttctgg
     8881 agggaaacat cctggccggt ttccatcgtc ctctgctgaa agccaacctt gccgcaactt
     8941 tgacccgccc ggcagccggc gtcgatttca ttcggctgga cggcgccttt aaaccggggc
     9001 ggcgccccgg cgaaatgaac ggaagcctcc aggttgacgg cctgcgcaat acccggccga
     9061 tcctgcggat gtcatcggac ctcggcatga cgcctgcggg cctgtccttc tccaaaatcc
     9121 gcctgcggcg cgagaaccgt ccgggatccg ttgccggcga aggcctgctg actttcaaag
     9181 gcccgacccc ctgcataacc atgagaattc aggcggaaaa cgtggatctt tccgacgagc
     9241 tgaaaatccc ggcaaacctc tccggatcgc tgctctttga gggaaccggc cggcaatatc
     9301 agggtcgcgt cacgctggcc aatacagcgg acacccgcca tgctttccgt ctggccggtg
     9361 attattccgg ggattccgaa agcgccacgc tcagccggat tcagggaaca gccctgggcg
     9421 gatccctgaa cggacgcctc gagatccggt ggacggacgg cctgcggctg aacggcgcga
     9481 tgtcgggaaa aaatctcgat ccggcaagat gggatccgga atgggcgggc gtgatccatt
     9541 ttgatcttgc aggcgacgta tcggctaagg cgggagagcc ggcaagcggc gcattgaatc
     9601 tcacgctgaa agaaagccgc ctgcacggaa ggcagttgac gggagacctg cgggcaagct
     9661 ttgcgggcga cgacatcgat attcatcatc tggcgctgag gggaaaggga tttgaaatct
     9721 cggccagggg aagtattgcg gatcaattgg atttcaaggc tgaaattcgc gatctggcga
     9781 ggctgattcc gcgcgcttcg ggagctctta cggcttcagg atgggcgcgc cggcgtcatg
     9841 accggttcag cggcgtcgtt tccgcgcggg ggcacagtct gtcggcggac ggctggtcca
     9901 tcgccagtgc gaacttcagc gcctttctgg acgaccggga aaagtccccc ttctcactga
     9961 ccgcggcttt cagccggctt cgctttcaga ctctgacagc ggatacgctg acgctgcggg
    10021 gcgggggaac cgtcctttcc cacacattgg acgcttttac gcgccgcgga cctcacgaaa
    10081 tgcatctggg cctcacgggc gcctggcgca gaaaaggctg gcagggaaaa atcgttcggc
    10141 tggaaggcag agatggcgtc ggcccctgga gactgtccgc gccggccgcc ctggccgttt
    10201 cccgggagag ccttgcgctg gggccgatgg aaatcacagg ccgtgggccg gaagcgctcc
    10261 gcctgtccgc ggcgctttcc ggaaatcctc tgaccggtcc tgtcgatttg agatggaaca
    10321 gcctcgatct ggccagagcc aatgcctgga tcgatcgcga catcctcgcc ggagtctgca
    10381 gcggaagcct ccaggcaacg ctgctgccct tcaaccgcat cgaccttgcc ggggagacga
    10441 ccctgaccgg aacgctccag acgcaggggc aaaccgttga gatcaggcag gggaaaatga
    10501 ccctgaacgc ggacaggcgg ggagtccggc tggacatgaa gattgagcca tcggaaggcg
    10561 gagcgctgtc cggcgctttt gtttcctccg ggcccgccga acggtcgatt cccgaaacgg
    10621 gagaatggaa tctgcgctgg cgggaactgg atctgaaaag attttcggcc gcgctccccc
    10681 gccgcacgcg tctggaaggc aaaatatcgg gggagatgaa tggaacccta ttgccggaca
    10741 gacgcttttc tctttcgggc cgcgccggcc tggacgggtc aaaaattaac cggcggggac
    10801 agaggggcga cgtcagcatc gatcttcgcc gggcatccat tcactggctc tggcagaaag
    10861 actatctgga atgcgaactc cacgtgacaa tggcggacct cggtgcactg cagggaaaaa
    10921 tccgtctgcc gctggctgcg cactttccgc tggtcatgaa cgaacaggga aaccttcagg
    10981 gttcgcttat gggacaggtg caggaaaagg gcgcgctggg cgttcttttt cccggactgg
    11041 ttcaggagag ccggggaaat ctcgatctgg acatgaaaat gagaggaagc ccggcggacc
    11101 cgctctttga aggccgtgtc cgcctgtcca aagcgggagg ctatctgccg tcggccggta
    11161 tcacgctgca ggatgtccgg atcgacgccc gtctggcgaa agacggcgta accatcgatt
    11221 ctttccgtgc tgtttccggc ccgggttatc tggacggcac cgcacgaatt cgcctcaaag
    11281 ggtttaagat cgcggatttc gaaggaaacc tgaacggcga tcggtttcag gccatccacg
    11341 ttcccgaact tcacgtgcag acgtctcccc gcctgacctt ctcgggaacg ccggaaaaaa
    11401 tatccgtccg gggagaagtg ctcctgcccc tgctgcgggt caacggatcg caatcgcagg
    11461 gtcccgtgga atccagccct gacctggtcc gggaaggaaa aacaaagtcc ggaaccggaa
    11521 agccgtccgt tggtctcgat gttcaggtca aactggtgct gggcgaacag gccgagttca
    11581 acgcggccgg aatcgacgca aagctgggcg gcgaaatgga gatccggttc agcgatccgg
    11641 agcgcatttc gggcaggggt gaaatccgcg tggtcaaagg ccggttccgg acctacggcg
    11701 tgaacctgga aattgtccgc ggccatcttt tctacgcggg aggtccggtc aaccagccgg
    11761 cgctggacat tctggcgatt cgaaaaatag gagaagtaaa agcgggcgtc gcggtcagcg
    11821 gcaagcttcc aaaacccttg gtcaaattgt attcggaacc ctacatgcag gatatggata
    11881 ttctggctta catcgtgctg gggcatccgc tcggcgccga ctcaaagcag gccggccttc
    11941 tggccacggc ggccggcgcc gttttgacct cgcagcaggc cgaagatctg ctggagcaga
    12001 ttcaggacta tctggggctt gcctctctgg aaatctcctc ggatgtgctg gagaaaagca
    12061 accagatggg gtataagcgc ctgaaagtaa cgcccgaggg aacaggcagt tcgtcgccag
    12121 gcgcttcgga aaccattctg gtcgtgggca agtatttgac gcccgagttg tacatcagct
    12181 acggccgctc gctgttttcg gggggcaatt tattttttct gcgctacgat ctttccaaaa
    12241 actggcaggt ggaaagccag atggggcagg aaagcggcat cgacctctac tacaaactgg
    12301 aatttcacta aagggagaga caaccatgcc atcgcgccat tttgatctgg ccatcgccgg
    12361 cggcaccctg gtgaccatgt cctccaggat ggacatcatt gaaaatgctt ccatcggcat
    12421 atccggcggc cgcatcgccc tcgtggacac aaaccgcacg ctcgcggccg acaggatcat
    12481 cgacgcccgc ggcggcttga tcctgccggg gctggtcaac acgcatacgc atctgccgat
    12541 ggtctgcttc aggggcctgg cggacgacct gccgctcatg gactggctcc atcatcatat
    12601 tttccccatg gaagcgcgct tcgtgaacaa gcagatggtg cgcgacggcg ccatgctggc
    12661 cattgcggag atgatccttt ccggaacaac cacctgctgc gacgggtatt tctttgaaaa
    12721 tcagatcgcc gaagccgcgc tggccggcgg catgcgcgcg gttgcggcgc agggattcgc
    12781 cgactctgcc acgccggacc atccccagta cgaaaaaatg atggcggcgg ccggacggtt
    12841 cgtggaacgc tggctccccc acgcgccgat gatcacgccg gcatttttct gccattcccc
    12901 ctacacctgt tcaccccgga cgctggtcaa cgtgaaggcg gccgcgcgcg aggccggaat
    12961 cctttacctg acccaccttc tggaaaaccg ggatgaaatc gatacgattg aaaaacgcta
    13021 cggccggaag ccggtgcagc atttgcacca ccttgacgtg ctggatgacc ggaccgtagc
    13081 cgtgcactgc aactggatga gccctgaaga catcgcgctt tttgccgatc tgggcgtcag
    13141 cgtgtcccac aacccggcca gctccatgaa gctggccgcg ggcgtcgcgc cggttccgct
    13201 gatgctggag aagggcatcg cggtcggttt gggcacggac ggatccgcca gcaacaacga
    13261 cctcgacctg tttcaggaaa tggatctttc ggcaaaagtc cacaaagcga ctctcctcga
    13321 tcccacggtc atgagcgctg aaaccgtcct gaaaatggcg acgatcgaag gcgcccgcgt
    13381 tctcggaatg gatcggctga tcggctccgt ggaggtgggc aaacaggcgg acatcattat
    13441 cctggatatg aaccagccgc atctgacgcc tctttacaac ccctactctc agattgttta
    13501 tgccgcgcgg ggcggagacg tgaaaaccag catcatcgac gggaaaccgg tcatggagaa
    13561 ccggcggctg ctcacgatgg acttgccggc cgtgatggag cgcgttcgcg ccatcgcggc
    13621 gcatattcag tcggaacatc aatccgcgat cagaacctca ccggttgatg tctgacgcca
    13681 taaatagctg gagaggaacg gtttaaaacc gtcccctgcg ttgttatttg cccggtgaaa
    13741 ataatttcag ataacggccg tagccttctt tttccagatc ttccttggaa acaaaacgca
    13801 gagccgcgga attcatgcaa taacgaaggc ctgcaggccc cgggccgtcc ggaaacacat
    13861 gcccgagatg ggagtccgcg tgtctgctgc gaacttccgt gcgcgcctca aaaaagctgc
    13921 ggtcctcttt tgtcaaaatg ttggccggct ccagcggccg cgtgaagctg ggccagccgg
    13981 tgccggaatc atatttgtcc agagaactga acagaggctc cccggacacg acatcgacat
    14041 agatgccctc ttgtttgttg tgaacatatt cgttttgaaa gggcggctcc gtcgcgcttt
    14101 tctgcgtcac attgtattgc atgggagaaa gcgtcttctt cagggtctga tcgtccggtt
    14161 ttcggtagag cggcgttgaa gccggtgtgt tgctctgatc cttcttccaa acttcccgga
    14221 gaaaaccttc ccgtcccgac ccgctgcggt aggccctgta gcgcagcggg tttttctcgt
    14281 cataatcctg atggtaatcc tccgccggat aaaaacccgt aaaaggtttc agctccgtca
    14341 caatcggctt gccgaacttt ccggatgcgg caagcgcctg cagagctccc gcggctgtct
    14401 ttctttccgc ctcgtcgttg taaaagacgg cactgcgata gccgggaccg cgatcggcaa
    14461 actgccctcc cgcgtccgtg ggatcaatat gctttagaaa gtaagtgagg atctgctcgt
    14521 aggtcaccac tttcggatcg taatgaacct gcgccgcttc caggtgcccc tgagccatat
    14581 aggtctcgta agtggggttt ttctccgaac cgcccgcata gcccgaaaca acccggatca
    14641 cgcccggcag tttcttcata tcggaagcca cgcaccagaa gcaaccgccg gccagcgtcg
    14701 ccaccgccgc attttccgtt tggatcatac cggtcatttt acccgcccct tccgccggct
    14761 gtgtcttgcc gccctggcac gccattacaa acagcgccag tcccgcggcc atccatgcct
    14821 ttttcatttc gtctgcgccg tatttggaat acggttctcc tttagtgaaa gtgtaaggac
    14881 gattgcggaa atgtcaaacc ggcaaaaaaa agaggccgtg aaggggcctc tttttttgcg
    14941 tcgctgccgc gaccgctttc attgagaagg ctgccgaagc ggcatcgggc atctgttaaa
    15001 acggctactt cttctttttc tcggccgcct tcttttcttc cttcgcttca attttggtcg
    15061 cggtcatcgt gtattcgatc gtgaccttgg agccgacttt cacatcgcct accaccttgg
    15121 ttgcagcgct tttggccact tcccagttgt cgctcccttt tttgacgaca atcacatctc
    15181 ccttcacgtc cagaaccggc cctgtcacct gataggtttt gggccccgcg gccaatgcca
    15241 ctgtcgccac caataaaaac atgcaggcta aaatcaatac ttttttcatc cgtccatcct
    15301 cctttttaat gtttaagatt tcatccctat tcaacgataa tgcgcccctt gatcgcatca
    15361 tacgtctttc cctttatgcc cttgatattc atgacatctt caatattccg gtacggcctg
    15421 ccgtcgataa tcgctttggc cttgacgggg ccgatcccca aaagcttttc cagtgtggcc
    15481 tggtcggcct tgttgatatt gactctcacc gccggagcca gtttttccgc ggcctctttg
    15541 ggggcggcca gcgcggatgc cgccgccgca atcagagcgg cacaaaggat caaggcggca
    15601 aacaaaagac aaccatttct caaacacaaa aaatgctttt tcttcaacat aagacctcct
    15661 tgtatgatat ttttacaatg aagcgccgga cgcagagact tttagcagag accggtggac
    15721 ttgtcaaaga gaatgtcgtg gcggttgagg caacgaacct cttgacttta tttgtttttt
    15781 cggttagctt ttccggcaaa aacaggcgga ccatggctga ccctcaaaaa caaaaacttc
    15841 gtttggacta tccgtgctca tggacctata aaatcatcgg ccggaatccg gatgatatgc
    15901 aaagcgcggt tcatgaagtt gtccgcgatc gttcctgccg ggtctccctg tcgcgtcaaa
    15961 gtgcaacagc caaatatgtt tgtctccata ttgaactgac ggtggagagc gaatcccacc
    16021 ggctcgattt gtatgagaaa ctcaaaacgc atccggccat tgacgtaatt ctctagaaac
    16081 cgttttgcac cggcgccccc tgaaagcagg tcagggcgga aacggtcttg ttgcccaaat
    16141 atctctttga gcggccattg agagcgtttt gttctataaa tcttggcgaa gcgaaagatc
    16201 agcaatgaca actgtttgag ccgaaggcga
//
LOCUS       DAOSRM010000046        16014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3749_length_16014_cov_113.773294, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000046 DAOSRM010000000
VERSION     DAOSRM010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 16014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16014
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3749_length_16014_cov_113.773294"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLA58_05995"
     CDS             complement(<1..676)
                     /locus_tag="PLA58_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOR62383.1"
                     /translation="MPREDLSKLKIDSSARKNVAGGRKKKPFLIAGAMILLAVGVILY
                     RLGVIAPSATVDVTTVSMIYPAQSLSVLNASGYIVAQRKAAVASKVTGRLTELLVEEG
                     SRVRKGQILARMESGDVAAMKEQGTANLANARAMLKQALVERDHAAQENARYQKLIAG
                     GYVAQSDYDMIHTRYLRSLEAVKAAQAAVESAGAALAHAEVNLDYTLIRAPFDGVVLT
                     KNADVGD"
     gene            892..>2931
                     /locus_tag="PLA58_06000"
     CDS             892..>2931
                     /locus_tag="PLA58_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOR62384.1"
                     /translation="MTPEENHRPESFAWLAKRSNVVRLALIFFLILSAASFFLIYGHY
                     RDAREQVFREDRKSAKLISLIVEAYLNEIRSTLESYASRPLLLEAVRRRDVLAAKQHL
                     ASLNRVSPGMDSLLIADKLGTVWISLPDRPDVLGKNFAWRDWHRGVIREKKPYVGDAV
                     LRVTGEKDAAIHIAVPIFDKKKEIIGILLNATRAVEIGRVLRRAPLEEGLSISVTDRN
                     GTLIYSSRYAYAKTPGRYPFFDVVKKTTGAELSTVAFPDPDERGNMLHISRAILPGAG
                     LHVFVERDQRSIWSSVWSHLVQMTAISTLLFLCVIMLVLYIRKRTIAQSALERLETER
                     EISAGRSRFSELFNQLKSAVAIYQAIDGGKDFEILELNAAARRITKVDRNVKGKSVRD
                     IYPGLINHTLLTTFQNVWRTGEPEEHPTFLYQDDKQTFWTDNYVFKLPSGEVVSVFED
                     VTARKLAADKISRQTRLLSAINRLFAETLKSRDREAVARTCLAAAQSITNSELGFIGE
                     VSPDGLFNTTAVSDTGWRACAIPQKEATAVLQNMIIRGVWGQVILRQQSLIVNDPGAF
                     PDRVGIPAGHPPLTSFLGVPLKDEGKTIGMIALANRAGGYTGEQREDLEELAVSLVEA
                     LRRNKAEEEVRKMNLELEKRVVERTEDLAAKTAELERINRVFVDRELKMRELKARI"
     assembly_gap    2932..3031
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3032..4714
                     /locus_tag="PLA58_06005"
     CDS             <3032..4714
                     /locus_tag="PLA58_06005"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR62385.1"
                     /translation="DGRSVMLSLCALFFGPLAAAVAGLMTLICRISMGGVGLIMGILT
                     TLSSMTIGLVFFYRCKPLLTPPSTMLLYAMGLAVHAAVAALMFTLPGDLAWNTFKNLA
                     LPILLLFPLATLLAGKILSDHLSMLQSASALSESEERYKLLFEASLDGVLLTAPNGRI
                     FSANPALCRLLGRSEEEIIALGRGGVVDREDPRLAEALAERDRTGKFAGELTMIHKEG
                     TKIPVEITTAVFKDRFGQARTSMIIRDITRRLQEEEPLKKLQALLAETQRLSKIGGWE
                     CDLTTGLSRWTEEVYKIYGISPDAQLSAEDAMDFYAPEDRPVIAAAFRRLLETGESYN
                     LNLKFNNASGEPLRVRTSGQAEWKDGKIVRVFGHIMDITERARMEDALRESEELFRNL
                     FMRHEAVKLILDPETGDIIDANNAAEKFYGWSREQLLTMKIFDINTLTQAEIRKALQT
                     VKDQKRTRFQFRHRRADGAIRDVEIFSSRIDVKGKPLLHSIITDISERKAAEIRMLQI
                     NEELEQKVQQRTAELTRTIAQLEETNRVFVGRELKMAELKKRIEELEKSGKS"
     gene            4752..5405
                     /locus_tag="PLA58_06010"
     CDS             4752..5405
                     /locus_tag="PLA58_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR62386.1"
                     /translation="MATKRILIVDDKDENRYLLQALLQGSGYEVFTAVHGADALEKAR
                     QDPPDLIITDILMPVMDGFALCRAWMKDERLQSIPLIFYTATYTDERDREFALSLGAR
                     HFIVKPAEPDDFMAVIHGVFESLEQPTAAPEGKLEEASEESVYLKQYNEVLIRKLEGK
                     MEQLEKLNQDLEKKVAQRTAQLKESLERLEELNRVFVGRELKMAELKKKIEDLERKG"
     gene            5417..7669
                     /locus_tag="PLA58_06015"
     CDS             5417..7669
                     /locus_tag="PLA58_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR62387.1"
                     /translation="MTLDMRTVTTSLVITAVICVVLMASFWWQNRKRFEGLHLWLLVF
                     LLHALGMILIALRGSVSDWASVVLASILLLAGTLLMYILFLKFIGEKKRQLPNYIILI
                     LLSLVQVYFTFIQPNLSARIFNFSAGVLIFTSQAAWSALYRVRSDLRPLTLGIGLVFS
                     LECLISIVRMIDCLINTLHPTDFFQSGSVNVLSFIGYQTLLILLAYSLTLMFNKRLLW
                     NIEKQEEKFSKIFYLSPNAINLTRLSDGVILEANEGFYRASGYSREETLGKKTTDLHL
                     WAREEERLQFVRDLARNGSVHNREFLFRIKTGGTLTGLFSAGIIEIDRVKYALTILTD
                     ISDRKKMEEELKAGRDYLEKMNNTLADALFVGKYPEQEFEYLNDAAVRLFGYSRQEIV
                     GKSSLLLFPDEKGYHHSGAIFEETIAHQKSIVRFETTLKKKNGEIFPALLSAAFLEEK
                     GAVVRFIIIVRDISEQKQNIEEIHKLNEKLESRVAERTEALSDMQMALLNLVDDLNES
                     SREISSANKYLEEVNRELAAFSYSVSHDLRAPLRAIDGFSNALLEDYSDRLDAEGKIY
                     LEKIQHAAQRMNLLIDDLLALSRVMKADFYRQNFDISEMVREIAADFQQQSPSGKLEL
                     VIQDGVTVNADQRMMRIVMTNLLDNARKFAGKQERPRVEFGSFVQNGDTVLFVRDNGV
                     GFNMAYVHKIFEPFERLHRADAFPGTGVGLATVQRVIQRHNGRIWAEAEEGKGAVFYF
                     TLGDSKCQNR"
     gene            7654..9045
                     /locus_tag="PLA58_06020"
     CDS             7654..9045
                     /locus_tag="PLA58_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR62388.1"
                     /translation="MPKPLNVLLIEDSEDDALLLARALKKNGYEPRIERAQTARDMSR
                     ALESAPWDIILCDYHMPAFSGLEAIDLLKKTGLDIPLIIVSGAIGEETALDCIHRGAA
                     DYIMKGNLTRLGMAVQRGLEEKAMRDHHRQDEEALRRSEEKYRTILEGIGEGYYETDL
                     AGNFTFFNDALQRMWGYPKEELLGMNYRQYTDLETAKMLYEGHHQTYLTGIPGRYMDY
                     EIIRKNGERLTLRQSFTLLKDSRDGIIGFRGIVRDVSHLKKLERDREETLEKLRQALE
                     STVHAMAMAVEARDPYTAGHQRRVADLACAIAAEMGLEPDRLNGLRMASLIHDLGKIS
                     IPTEILTKPTKLTNIEFEIVKTHAQAGYEILKDIVFPWPIARIVLEHHEKIDGSGYPR
                     RLKGDALLPESKILTVADAVEAMASHRPYRPSLGLQDALEEISRNRGILYDTDAVAAC
                     LKLFEEKRFAFAI"
     gene            9165..11330
                     /locus_tag="PLA58_06025"
     CDS             9165..11330
                     /locus_tag="PLA58_06025"
                     /inference="COORDINATES: protein motif:HMM:NF022783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HOR62389.1"
                     /translation="MTSPKSGTKASPWADWQKHLNDYLVDSAQRSIIFLDILRKRGNN
                     YIQHIRSGQPPVLTFNYEMILNAKHFERPVNYALVRISERRRKDIDPDRQNRRRALRE
                     RPDIPKRPIIIIDPRAGHGPGIGGSKRDSEIGMALDHGHPVYFVLFYTDPIPGQTLPD
                     VIFAIEQFIAIVAELHPDADAPAVIGNCQAGWAAALACADKPGIAGPLALNGAPLSYW
                     SGVAGVNPMRYRGGLLGGVWVNSFLSDLGNGVFDGANLVLNMELLNPANTYWTKQYNV
                     YSNVDTEEDRYLNFEKWWGGFFMMNDEEIHYIVRNLFVGNKLERGELELQEGRKLNLK
                     DIESPILVFASMGDNITPAQQALNWIPRVYSSVEDIRRHGQVVVYIVHQEIGHLGIFV
                     AGSVAKKEHREIIGNFDMIDCLPPGLYEMVIDGDVASGKFESRFEPRTMADIAAYDDG
                     IQDEKDFSVVAKVSEANDNLYRLLARPWIKLWTTELSAEFIRHLHPLRLQRYSLADIN
                     PLTRPLKNIANVVRKNRKPAGPDNPFTELEKAVSDYVEGVLDIYRDYRDLHQEEIFQL
                     IYGNGWLRSLFPPVQDEPRPAAAKQDHPDYDKRLAAMEQGGVAEGLIRIYMAAAATNQ
                     GIKRKHFEVAREIAATHRVLSKLSLSRFKKIMREQSAILQADEEKALQTLATLIKNKD
                     DRMEALSIARRLFLADGVYNDEEKALLEKIRKGLGLEAV"
     gene            complement(11354..12349)
                     /locus_tag="PLA58_06030"
     CDS             complement(11354..12349)
                     /locus_tag="PLA58_06030"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOR62390.1"
                     /translation="MPNASTAGKAARKKENSLTIVEVSPRDGLPEVCSGLSTEDKINY
                     INKLSETGLKKIECASFTHPRLLPEGYDAEKLVAGIAKKPEVTYVGLVPNEIGCRRAL
                     VTQIDELLVLLSASDTFNRINVGKSLKESLNKVLPTLLDAAVRNARSVRIYVMTAFGC
                     PYTGPIRPEEVARLLLKLSYMGASEVVLVDSTGLADPKSVKELIRMLLDLKLGIQLAV
                     HFHNTRGTAIANCVAAYEAGIRIFDASLGGMSNTPYGVSELDIGYWNVPTEDLVHLFE
                     AMGIQTGIDLEKLLDCVRLAEKMAGKPLPGHILRARPASQITEIPRHLKGQPVLD"
     gene            complement(12417..13403)
                     /locus_tag="PLA58_06035"
     CDS             complement(12417..13403)
                     /locus_tag="PLA58_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOR62391.1"
                     /translation="MDASLVESAFDELIGVDETFRKALVSAGRAAQSDISVLIIGESG
                     TGKEILARAIHRASRRKNQQLVDVNCAAIPDSLIESELFGYEKGAFTGARTEGRKGYF
                     DEAHEGTILLDEIGDSSLSVQAKLLRVLESGTFKRVGGTKNIKVDVRVISSTNQDLAD
                     LIGQKKFREDLFFRLNTFTLTLPPLRERRGDIDLLVEHFLKASGAAQKLKYRFSPEAL
                     AMLHAYHWPGNVRELKGVVSYAVNMTQSSVIDAGSLPNFLFSDPAGPDGAPPSVAASG
                     PVSLNLTEAVRDLERRLIAEALAAFPNRSEAIRALGISRRTFYLKLKEYGLE"
     gene            13659..15743
                     /locus_tag="PLA58_06040"
     CDS             13659..15743
                     /locus_tag="PLA58_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOR62392.1"
                     /translation="MAEIVTFSKEGPTGVITVNNPPVNALSQAVRAGIKAGIEKGIAD
                     ADISAMVIICEGRTFIAGADIREFGRPPMEPHLPDVCQFIEDSPKPVIAAIHGTALGG
                     GLEIPLSCHFRIALPTAKVGLPEVKLGLLPGAGGTQRLPRVAGARSALEMITSGAPVA
                     AARAKEMGILDEIVEGDLKAAALAYAQKVVDQKLPIRRVSQLKANVESPTLFEDFAKA
                     NARKFRGFLAPLKCIEAVKIATELPFPEGMKKEREIFIELHDSDQSKAQRHVFFAERE
                     VVRIPGLPDDHPTREIRSVGVLGAGTMGGGIAMCFANAGIPVKLLDMKQEFLDRGLAV
                     IKKNYANTVAKGSLKQEVMDKRVSLIQPTLAYEDLKDVDLVIEAVFEDMAIKKEVFTK
                     LDAVCKTGAILASNTSYLNIDEIAAITKRPQDVMGMHFFSPANVMRLLENVRGAKTSP
                     EVYATAMKVGKKIGKVPVLVGVCDGFVGNRMLAKRTRECGFMLEEGALPWQIDRVIYN
                     FGFPMGPYAMGDMAGLDIGWRNRKAKFDSLSPREQKNNILDKICEMGRYGQKTGSGFY
                     KYDEKRNATPDPMIEELIINHSKEVGIARRTITDQEIIERAIYSMINEGAKILEEGIA
                     ARPLDIDVVWLYGYGFPVYLGGPMFYADTIGLGKVYDAVLKYRDLVGAEYWTPAPLLE
                     KLAKEGKGFYSK"
BASE COUNT         3624 a         4590 c         4509 g         3191 t
ORIGIN      
        1 tgtcgcccac gtcggcgttc ttggtcagca cgacgccgtc aaacggcgcc cggatcagtg
       61 tatagtccag gttgacctcc gcatgggcaa gcgccgcgcc ggccgattcc acggcggcct
      121 gcgccgcttt cacggcctcc agcgaccgca ggtaccgggt atgaatcatg tcgtaatccg
      181 actgcgccac ataaccgccc gcaatcagct tttgatagcg ggcattctcc tgcgcggcgt
      241 gatcgcgctc caccagcgcc tgtttgagca tggcccgggc gttggccagg ttcgccgtcc
      301 cctgttcctt catggcggcg acgtcgccgc tctccatccg ggccagaatc tgtcccttcc
      361 ggacgcggct gccctcttcc accagcaatt ccgtcaagcg gccggtcacc ttggacgcca
      421 ccgccgcctt gcgctgggca acaatgtagc cgctggcatt caggacggac agggactggg
      481 ccgggtaaat catcgaaacg gtggtcacat ccaccgtggc cgaaggcgca atcacgccca
      541 gacgatagag aatgacaccg accgccagca aaatcatcgc gccggcaatg agaaacggct
      601 tctttttccg gcctcccgcc acgtttttcc tggccgatga atcgattttc agcttgctca
      661 aatcttcccg cggcatgttt ttctcacctt aaaagattgt gatttccggg aacaataaca
      721 gaactgagca gaatttgcca tacgcatgaa aacccttttt acttcttgac aatggagagc
      781 gttgttttca taatgcccca aatcgatctg ttcggaaaaa agtttctttg caaccggacc
      841 taaaagggtc ggccgttttg ccggcctgac cacaggacca taaggcgcct catgaccccg
      901 gaagagaacc atcgacccga atcctttgcc tggctggcca aacgctcgaa tgtggtccgg
      961 ctggccctga tctttttcct cattctctcc gcggcttctt tttttctgat ctatggccat
     1021 taccgggatg ccagggaaca agttttccgg gaagaccgga aatccgcaaa gctgatttcg
     1081 ctgatcgtcg aagcctatct caacgagatc cgatccactc tggaatccta cgccagccgc
     1141 ccccttctgc tggaagccgt ccggcgcaga gacgttttgg cggcaaaaca gcacctggcc
     1201 agcctaaacc gggttagtcc cggaatggac agcctgctga tcgcagacaa gttgggcact
     1261 gtttggatca gtctgcccga ccggccggac gttctgggaa aaaacttcgc ctggcgcgac
     1321 tggcaccggg gcgtcatccg ggaaaagaag ccctatgtcg gggatgccgt tttgcgtgtc
     1381 acgggcgaaa aagacgcggc catccatatc gccgtcccga ttttcgacaa gaagaaagaa
     1441 atcatcggca tcctgctcaa tgccacgcgg gccgtcgaga tcggtagagt tctccggcgc
     1501 gcgccgctgg aagaggggct gtctatttcc gtcacggacc gcaacggaac tcttatttac
     1561 agcagccggt atgcttacgc aaaaacgccg gggcgctatc cgttttttga cgtggtgaaa
     1621 aaaacgacgg gcgcggaact ttccaccgtt gcctttcccg atccggacga acggggaaat
     1681 atgctccata tttcgcgcgc catccttccc ggcgccggac tgcatgtttt tgtcgaacgc
     1741 gaccagcgtt ccatctggtc gtcggtatgg tcccacctgg ttcagatgac cgccatttcc
     1801 accctgcttt tcttatgtgt gatcatgctc gtattgtata tccgcaaacg caccatcgcc
     1861 cagtccgcac tggagcgcct ggagacggaa agggaaattt cggccggccg gtcgcgtttc
     1921 agcgaacttt tcaaccaatt gaaaagcgcc gtagccattt atcaggccat cgacggggga
     1981 aaggattttg aaattctgga gctcaatgcc gctgcccgcc ggatcacaaa ggtggatcgg
     2041 aacgtcaaag gcaaaagcgt ccgggacatc tatcctggtc tgataaatca tacgctcttg
     2101 accacttttc aaaacgtctg gcggacggga gagccggagg aacatcccac ctttctttat
     2161 caggacgaca aacagacctt ctggacggac aattacgtat tcaaacttcc ctccggcgaa
     2221 gtggtttccg ttttcgaaga cgtgaccgcg cgtaaactcg cggcggacaa gatttccagg
     2281 cagacgcgcc tgctttccgc catcaaccgg ctttttgccg aaacgctgaa atcccgggac
     2341 agggaagcgg tcgcccggac ctgccttgcc gccgcgcaaa gcatcacaaa cagcgaactc
     2401 ggatttatcg gcgaagtgag cccggacgga cttttcaata cgaccgccgt cagcgacacg
     2461 ggctggcgcg cctgcgccat ccctcaaaag gaggcgacgg ccgtccttca gaacatgatc
     2521 atccggggcg tgtggggaca ggtgatcctc cggcagcagt ccctgatcgt caatgacccg
     2581 ggagctttcc ccgaccgcgt gggcattccg gcggggcatc cgccgctgac gtcctttctg
     2641 ggcgtaccgc tcaaagacga gggaaagacc atcgggatga tcgcgctggc caaccgcgcg
     2701 ggcggctata ccggcgaaca aagggaggat ctggaggaac tggcggtctc cctggtggaa
     2761 gccctgcggc gcaacaaggc ggaagaagaa gtcaggaaaa tgaatttgga attggaaaag
     2821 cgcgtcgtcg agcggacgga ggatctggcg gccaaaacgg ccgaactgga gcgcatcaac
     2881 cgcgtctttg ttgaccgcga attgaaaatg cgggagctca aggcccggat cnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngacggccgc tcggtcatgc tcagtctgtg
     3061 cgctctcttt ttcggtcctt tggcggcggc cgtggcgggt ctgatgacgc tgatctgccg
     3121 tatcagtatg gggggagtgg gattgattat gggaattctg acaacccttt cctccatgac
     3181 gattgggctg gtcttttttt accgatgcaa gcctctgctc actcccccat ccacaatgct
     3241 tctctacgca atgggtctgg ccgtccacgc cgccgtggcg gctctgatgt tcacgctgcc
     3301 gggagatctg gcctggaaca ccttcaaaaa ccttgctctg cccatcctcc tgctttttcc
     3361 gctggccacg ctgctggcgg gaaaaattct ttcggatcat ctttcaatgc tgcagtccgc
     3421 ctcggctctt tcggaaagcg aagagcgcta caagcttttg ttcgaagcca gcctcgacgg
     3481 ggtgctgctg acggcgccca acggccggat tttctctgcc aaccccgctc tctgccgtct
     3541 gctgggccgc agcgaagagg aaataatcgc cctggggcgc ggcggcgtcg tggatcggga
     3601 agatccgcga ctcgctgagg cgctcgccga acgggatcgg acgggaaaat ttgcgggcga
     3661 attgacaatg atccacaagg aagggaccaa aattcccgtg gaaatcacga cagccgtttt
     3721 taaagaccgc ttcgggcagg cccgcaccag catgatcatc cgcgacatca cccggcgcct
     3781 ccaggaggaa gagcccctga aaaaactgca agcgctgctt gccgaaacac agcggttgag
     3841 caaaatcggc ggctgggaat gcgatttgac aaccggccta agccggtgga cggaagaagt
     3901 ctataaaatc tacggcattt ctcccgacgc tcagctcagc gcggaagatg cgatggattt
     3961 ttacgcgcct gaggatcggc cggtcatcgc agcggctttc cggcgcctgc tggaaacggg
     4021 cgaatcctac aatctgaatc tcaaattcaa caacgcatcg ggcgaaccgc tccgggtgcg
     4081 caccagcggg caggccgaat ggaaggatgg aaaaatcgtt cgcgtcttcg gccatatcat
     4141 ggacatcacc gaacgagcgc gaatggaaga cgccctcagg gaaagcgagg aacttttccg
     4201 caaccttttc atgcgccacg aggcggtcaa attgattctc gacccggaga cgggagacat
     4261 catcgacgcc aacaatgccg ccgagaaatt ttacggatgg tcccgcgagc agctcttaac
     4321 gatgaaaatc ttcgacatca acacgctgac gcaggctgaa atccggaagg cattgcaaac
     4381 ggtcaaggat cagaaacgca cgcgctttca gttccgtcac cgccgggcgg acggcgccat
     4441 ccgcgacgtc gaaattttca gcagccggat cgatgtcaag gggaagccgc ttctgcattc
     4501 gatcatcacg gatatcagcg aacgcaaggc cgcggagatc cggatgcttc aaatcaacga
     4561 ggagctcgaa caaaaggttc agcagcggac ggccgagctt accaggacca tcgcccagct
     4621 cgaggagacc aaccgcgtct ttgtcggccg ggagctgaaa atggccgagc ttaaaaaacg
     4681 gatcgaggag ctcgaaaaaa gcggcaaatc ataagcgacg cgtgaaggac aggaagggag
     4741 aaaaagcagc catggcaacc aagcgaattc tgattgtgga tgacaaggat gagaatcgct
     4801 acctcctgca ggcgctcctg cagggcagcg gctatgaggt tttcaccgcc gttcacggcg
     4861 cggacgccct ggagaaagcc cgtcaagacc cgccggatct gatcatcacc gacattctga
     4921 tgccggtcat ggacggattc gcgctttgcc gcgcctggat gaaagacgaa cgcctccaat
     4981 ccatccccct gatcttttac accgccacct ataccgatga acgggaccgg gaattcgcgc
     5041 tctccctcgg agcccgtcat ttcattgtca agcccgcgga gccggacgat ttcatggcgg
     5101 tcatccacgg cgtctttgag tccctggagc aaccgaccgc cgcaccggaa ggcaagcttg
     5161 aggaagcgtc cgaagaatcc gtttacctca agcaatacaa tgaagtgctc atccggaagc
     5221 tggagggcaa aatggagcag ttggaaaaac tcaaccagga tctggaaaag aaagtcgccc
     5281 agcggacggc ccaattgaag gagtccctgg aacgcctgga agagctgaac cgcgttttcg
     5341 tcggacggga gctgaaaatg gccgagttga aaaagaaaat tgaagacctg gaaagaaaag
     5401 gctgaggagc gccgccatga cgctggacat gagaaccgtc acaaccagtt tggtcattac
     5461 cgctgtcatt tgcgtcgtcc tgatggcgtc cttctggtgg cagaaccgca aacgctttga
     5521 ggggctccac ctatggctgc tcgtttttct ccttcatgcc ctgggaatga ttctcattgc
     5581 tttacgcggt tccgtctcgg actgggcgtc cgttgttctg gccagtattc tgctgctggc
     5641 cgggacgttg ctgatgtaca ttctgtttct gaagtttatc ggagaaaaaa aacgccagct
     5701 tcccaattac atcatcctga tcctgctgtc tctcgttcag gtttatttta ccttcatcca
     5761 gccgaatctg tcggcgagaa ttttcaattt ctccgccggt gtgctgattt tcacatctca
     5821 agccgcctgg agcgcactgt accgcgtgcg ctccgacctg cgccctctga cgctgggaat
     5881 cggtcttgtc ttcagcctgg aatgcctcat cagcatcgtt cgcatgatcg attgtctgat
     5941 caacaccctg catcccaccg atttttttca atccggctcc gtcaacgttt tgtctttcat
     6001 cggctatcag acactcttga ttctcctggc ctacagcctg acactgatgt tcaacaaacg
     6061 tctgctttgg aacatcgaga agcaggagga gaaattctcc aagatctttt acctgtctcc
     6121 caacgccatc aacctgacca gactgtccga tggcgtaatt ctggaagcca atgaaggttt
     6181 ctaccgggca tccggttatt ctcgggagga aaccctgggc aaaaagacga ccgacttgca
     6241 tctgtgggcc agagaggaag aacgccttca gtttgtcagg gatttagcca gaaacgggag
     6301 cgtgcacaac agggaatttc tcttccggat caagacggga ggaaccctga cgggcctttt
     6361 ttcggcaggc atcatcgaga tcgaccgggt aaaatacgct ctgaccatat tgacggacat
     6421 cagcgatcgc aaaaaaatgg aggaagaact gaaagccggc cgcgattatc tggaaaaaat
     6481 gaacaatacc ctggcggacg cgctgttcgt gggaaaatac ccggaacagg agtttgaata
     6541 tttaaacgat gccgccgtcc ggttattcgg ctattccagg caggaaattg tggggaaaag
     6601 cagcctgctg ctttttcccg atgaaaaagg ataccatcac agcggcgcca ttttcgagga
     6661 aaccatcgcg catcaaaaga gcatcgttcg ttttgaaacc accctgaaga agaaaaacgg
     6721 ggaaatcttc ccggcgttgc tctcggcggc ctttcttgag gaaaaagggg cggttgtccg
     6781 gtttatcatc attgtccggg atatttcaga acaaaagcaa aatatcgaag agattcataa
     6841 actgaacgaa aaactggaga gtcgggttgc cgagcgcacc gaggctttga gtgatatgca
     6901 gatggccctt ttgaatctgg tggatgacct taacgaaagc agccgggaaa tctccagcgc
     6961 caataagtat ctggaggaag tcaaccggga gctggcggcc ttcagctaca gcgtctcaca
     7021 cgatctccgg gcgccgctgc gcgccatcga cggattcagc aatgccctcc tggaagacta
     7081 cagcgaccgg ctggatgcgg aaggcaaaat atacctggaa aaaatccagc acgccgcgca
     7141 gcggatgaac ctgctgatcg acgacttgct tgccctctcg cgggtgatga aagccgactt
     7201 ctaccggcag aacttcgata tcagcgagat ggttcgagag atcgcagccg attttcaaca
     7261 gcaaagtcca tcgggcaaac tggagctggt gattcaggac ggcgtcaccg tcaacgcgga
     7321 ccagcgcatg atgcgcatcg tcatgaccaa cctgctggac aatgcccgga aattcgcggg
     7381 gaagcaggag aggcctcgcg tcgaattcgg gtcttttgtc caaaacggcg acacggtcct
     7441 ctttgtccgg gacaacggcg tgggattcaa catggcctat gtccacaaaa tttttgagcc
     7501 tttcgaacgc ctccatcggg cggatgcgtt cccgggaacg ggagtcggcc tcgcgacggt
     7561 ccagcgcgtc atccaacgcc acaacggccg tatctgggcc gaagcggaag aaggaaaagg
     7621 cgctgttttt tatttcaccc tgggagactc aaaatgccaa aaccgctgaa cgtgcttttg
     7681 attgaagatt cggaagacga cgcgctgctt ctggcccgcg cgctgaaaaa aaacggttat
     7741 gagccccgga tcgagcgggc gcagacggcc cgggacatga gccgggccct ggaaagcgcc
     7801 ccgtgggaca tcattttgtg cgactaccac atgccggctt tcagcggtct ggaagccata
     7861 gacctgctga agaaaaccgg cctcgatatt ccgctcatca tcgtttccgg cgccatcggc
     7921 gaagaaacgg cgctggactg catccaccgc ggagcggccg actacatcat gaagggcaat
     7981 ctgacgcggc tgggcatggc cgtgcagcgg ggactggaag aaaaagcaat gcgcgatcat
     8041 caccggcagg acgaggaagc gctgcgcaga agcgaggaaa aataccggac gatcctggag
     8101 ggcatcggcg aaggatatta cgagacggac ctggccggca actttacgtt tttcaacgac
     8161 gccctgcagc gaatgtgggg ctacccgaaa gaagagctgc tgggcatgaa ctaccggcag
     8221 tacacggacc tggagacggc taaaatgctg tatgaggggc accatcagac ctacctgacg
     8281 ggcatccccg gcaggtatat ggactacgag atcatccgaa agaacggcga acggctgacg
     8341 ctccggcagt ccttcacact gttgaaagat tccagggacg ggatcatcgg tttccgggga
     8401 atcgtgcgcg acgtctccca tctgaagaag ctggaaagag acagggagga aaccctcgaa
     8461 aagctccggc aagcgctgga atccaccgtt cacgccatgg ccatggccgt ggaagcgaga
     8521 gatccttaca cggcgggcca tcagcgaaga gtggcggacc tggcctgcgc gatagccgcc
     8581 gaaatggggc tggagcccga ccgactcaac ggtctgcgca tggccagcct gattcatgat
     8641 ctggggaaaa tatccattcc caccgaaatt ctcaccaagc cgaccaaact gaccaacata
     8701 gaatttgaaa tcgtcaagac gcacgcgcag gcgggatatg aaattctgaa ggacatcgtt
     8761 ttcccctggc ccatcgcccg gatcgtgctg gagcaccatg agaagatcga cggatcgggc
     8821 tatccgcggc gcctgaaggg ggatgctctt ctgccggaat cgaaaatcct gacggtggcc
     8881 gacgcggtcg aagccatggc gtcgcatcgc ccctaccgtc cctctctggg ccttcaggat
     8941 gcgctggagg aaatttcccg gaatcggggg attctgtacg acacggatgc cgtcgccgcc
     9001 tgccttaaac tgtttgagga aaagcgtttc gcctttgcaa tataacacgt cgcttgcggc
     9061 tcgtcactca tcgctcatcg cttctttccc gccttgacaa gggctaaacg cgccgctaga
     9121 gtacgttgac cgagaccggt taaagaatac aggaggattt tttcatgact tccccgaaaa
     9181 gcggcacaaa ggcctcgccc tgggcggact ggcaaaagca tctgaacgac tatctcgtcg
     9241 attccgcgca gcgttcgatc atttttttgg acattctgcg caagagaggc aacaattaca
     9301 tccagcacat ccgcagcggt cagccgccgg tcctgacgtt caactacgaa atgatcctga
     9361 acgccaagca tttcgagcgg cccgtcaatt acgcgctggt ccgcatttcc gaacgccgcc
     9421 gcaaagacat cgatcccgac cggcaaaacc gccgccgcgc gctcagggaa cgtcccgaca
     9481 tccccaaacg gccgatcatc atcatcgatc cgcgggcggg ccacggcccc ggcatcgggg
     9541 gctccaagcg cgattcggaa atcggcatgg ccctggacca cggccatccg gtttattttg
     9601 tcctgtttta caccgacccg attcccggcc agacgctgcc ggatgtcatc ttcgccattg
     9661 aacagttcat cgcgatagtc gccgaactgc atcccgacgc ggacgcgccg gccgtcatcg
     9721 gcaactgcca ggcgggctgg gcggccgcgc tggcctgcgc ggacaaaccg ggcatcgccg
     9781 gccccctggc gctcaacggc gcgccgcttt cctactggag cggcgtggcg ggcgtcaatc
     9841 ccatgcgcta ccggggaggc cttctgggcg gggtttgggt gaattcgttc ctcagcgatc
     9901 tgggcaacgg cgtgttcgac ggcgcaaacc tggtgctgaa catggagctg ctgaatccgg
     9961 ccaatacata ctggaccaag caatataacg tttattcgaa cgtggacacg gaagaggacc
    10021 gctatctcaa ctttgaaaaa tggtggggcg gcttcttcat gatgaacgac gaagaaatcc
    10081 actacattgt ccgcaatctc tttgtcggca acaagctgga gcgcggcgag ctggaactgc
    10141 aggaaggccg gaagctcaac ctgaaagata tcgaaagccc gattctggtt ttcgcgtcga
    10201 tgggcgacaa catcacgccg gcccagcagg cgctcaattg gattccccgc gtatacagct
    10261 ccgtggagga catcaggcgg cacgggcagg tcgtcgttta catcgttcac caggagatcg
    10321 gccacctcgg aatctttgtc gccggctccg tcgccaaaaa ggaacaccgc gaaatcatcg
    10381 gtaatttcga catgattgat tgcctgccgc ccggcctcta cgaaatggtc atcgacggcg
    10441 atgtcgcaag cggcaaattc gagagccggt tcgaaccgcg cacgatggcc gacatcgccg
    10501 cctacgacga cggaatacaa gatgaaaagg atttcagcgt cgtggccaag gtttcggaag
    10561 ccaacgacaa tctgtatcgg ctgctggcgc gcccatggat aaaactctgg accacggagc
    10621 tttccgcgga gttcatccgc cacctgcacc ccctgcgcct gcagcggtat tcgctggccg
    10681 acatcaatcc cctgacgcgt ccactgaaaa acatcgccaa cgtcgtgcgc aaaaaccgaa
    10741 agccggccgg gccggacaat ccgtttacgg agctggagaa ggcggtttcc gactacgtgg
    10801 aaggcgtgct cgacatttac cgcgattacc gggacctcca tcaggaagaa atctttcagc
    10861 tgatttacgg caacggctgg ctgcgcagcc tgtttccgcc cgtccaggac gagcccaggc
    10921 ctgcggccgc caaacaggac catcccgact atgacaagcg actggccgcc atggaacagg
    10981 ggggcgtggc cgaaggatta atccgcattt acatggccgc ggccgccacc aaccagggca
    11041 tcaaaaggaa acattttgaa gtcgcccgcg aaatcgccgc cacgcaccgg gtattgagta
    11101 aactgtccct gtcgcggttc aaaaaaatca tgcgcgaaca gtccgccatc cttcaggcgg
    11161 acgaggaaaa ggctcttcag actctggcga cgctgatcaa aaataaggac gatcgcatgg
    11221 aggcgttgag catcgcccgc cggctgtttc tggcggacgg cgtttacaac gacgaagaga
    11281 aagccctgct tgaaaaaatc aggaaggggc tgggcctgga ggctgtatag aagatataca
    11341 aaagcaggcc ccgtcagtcc agaaccggct gcccctttaa atgccgtgga atctcggtga
    11401 tctgggaggc ggggcgcgct ctcaggatgt ggccgggcag gggtttcccc gccatcttct
    11461 ccgccagccg gacacagtcg aggagctttt ccagatcgat gcccgtttgg attcccatgg
    11521 cttcaaacag atggacaagg tcttccgtcg gaacgttcca gtatccgata tccagttcgc
    11581 tcactccgta aggtgtgttg ctcatccctc ccagcgacgc gtcgaaaatg cggatgcccg
    11641 cctcgtaggc ggccacgcaa ttggcgatgg ccgtcccgcg cgtattgtga aaatgcaccg
    11701 cgagctgaat gcccagcttc agatccagaa gcatccggat cagttccttg accgatttgg
    11761 ggtcggccag acccgtcgag tccaccagaa ccacctcgga tgcgcccata taactgagtt
    11821 tgagcaggag ccgggcgacc tcctcggggc ggatggggcc cgtgtagggg cacccaaaag
    11881 ccgtcatcac gtaaatccgc acgctccggg catttcgcac cgccgcgtcg agcagcgtcg
    11941 gcagcacctt gttgaggctc tctttgaggg acttgccgac gttgatgcga ttgaacgtgt
    12001 cgctggcgga aagcagaacc agcagttcgt cgatctgggt caccagggcc cgccggcagc
    12061 cgatttcatt gggcaccaag cctacgtagg tcacctccgg ctttttggcg atgccggcga
    12121 caagcttttc cgcgtcgtac ccctccggca gcaaacgagg atgcgtaaag gaagcgcatt
    12181 cgatcttctt caggcccgtt tcggatagtt tgttaatata atttatttta tcttccgtgg
    12241 acaaaccgct gcagacttcg ggcaggccgt cccgcgggga cacctccacg atggtgagcg
    12301 aattttcttt ttttctggcg gcctttcccg ccgtgctggc attcggcata accgtcctac
    12361 accggaagaa ggattttttt catatttgca taattcattc cagccgaccg gaccctttat
    12421 tccaatccgt attccttcag tttcagatag aaggtgcgcc ggctgattcc cagggcccga
    12481 atggcttccg accggttcgg aaaagcggcg agcgcctcgg cgatgagccg gcgctccagg
    12541 tcccggacgg cctcggtgag attgagagag acaggcccgc ttgcggcgac agacggcggg
    12601 gccccgtcag ggcctgcggg atccgaaaac aggaaattgg gcagggaacc ggcatcgatg
    12661 acggacgact gcgtcatgtt caccgcatag ctcacgacgc ctttcagctc ccggacgttc
    12721 cccggccagt gataggcatg cagcatcgcg agcgcttcgg gcgagaaccg gtatttgagt
    12781 ttctgcgcgg ctccgctcgc tttcaggaaa tgctccacca gcagatcgat gtcccctctt
    12841 ctctcgcgca gcggaggcag cgtcagcgta aaggtattca ggcggaaaaa gagatcttcg
    12901 cgaaacttct tctgcccgat cagatcggcc agatcctgat tggttgaaga gatgacccga
    12961 acgtccactt tgatgttctt cgttccgccg actcttttga acgtgccgga ttccagaacc
    13021 ctgagcagtt tggcctgaac ggacaaagag gaatcgccga tttcatcgag cagaatcgtt
    13081 ccctcgtgcg cctcgtcgaa atagcctttg cgcccctcgg tgcgggcccc ggtgaacgcg
    13141 cctttctcgt agccgaaaag ttcgctttca atcagagaat cgggaatggc ggcgcagttg
    13201 acatcgacca gctgttggtt tttgcggcgg ctggcccgat gaatggcgcg ggccagaatt
    13261 tccttgccgg tgccgctttc cccgatgatc agcacggaga tatccgattg ggccgcccgc
    13321 ccggcgctca ccagcgcttt gcgaaaggtc tcatcgacgc cgatcagttc atcaaaggcg
    13381 ctttcgacca aagaggcatc cacacccggt ggggattctt ctttgccgcg cggtggggcg
    13441 gctggcaatt ttggggttcc ggccgtcata ggttcggttt tctttcctgc atgtttattc
    13501 ggtatacaaa tttcgccctg aatgtatata aaccatacac tcctgtcaaa cagtttttcc
    13561 ccggcgcggg tcagcgcccg cgccctattg ttttttttca ctggtatggt atttgctgta
    13621 ttcaatcaaa aaccatttgc aacaaaagga gatggcttat ggcagaaatc gtcacatttt
    13681 ccaaagaagg cccaacaggc gtcatcaccg taaacaatcc tcccgtaaac gcgctgtcgc
    13741 aggcggtccg cgccggcatc aaagcgggca tcgagaaagg catcgcggat gccgatattt
    13801 cggccatggt gataatctgc gagggccgga cgtttatcgc gggagcggac attcgggaat
    13861 tcggccgccc gccgatggag ccgcatctgc cggatgtctg tcagttcatt gaagattctc
    13921 caaaaccggt gatcgcggcc attcacggca ctgccctggg cggaggcctt gaaattcctc
    13981 tttcctgtca tttccgcatc gctcttccca cagcgaaagt cggccttccc gaagtgaagc
    14041 tggggctttt gccgggtgcg ggcggcacac agcggctgcc ccgtgtggcg ggcgcccggt
    14101 cggcgctgga aatgatcacc tcgggcgcgc cggtggcggc ggccagagcc aaagaaatgg
    14161 gcattcttga tgaaattgtc gaaggcgatc tgaaagcggc ggccctcgct tacgcgcaaa
    14221 aggtcgttga ccaaaagctt cccatccgca gagtgagcca gttgaaagct aacgtcgagt
    14281 cgccgacgct ttttgaagat tttgccaaag ccaacgcccg caagttccgc ggttttctgg
    14341 cgcccctgaa atgcatcgaa gccgtgaaga tcgccaccga acttcctttc ccggaaggca
    14401 tgaaaaagga acgggaaatc ttcatcgaac tccacgactc ggatcagtcc aaagcgcagc
    14461 gccacgtttt ctttgccgag cgcgaagtcg tccgcatccc cggcctgccc gatgaccacc
    14521 ccacccggga aatcaggtcc gtgggcgttc tgggcgcggg cacgatgggc ggcggcatcg
    14581 ccatgtgctt tgccaatgcc ggaattcccg tgaagctgct cgacatgaag caggaatttc
    14641 tcgaccgcgg gcttgccgtc atcaaaaaga actatgccaa tacggtcgcc aaaggctccc
    14701 tgaagcagga ggtcatggac aaacgcgtgt cgctgattca gcctacgctg gcctatgaag
    14761 acctaaaaga tgtcgacctc gtcatcgaag cggtctttga agacatggcc atcaagaagg
    14821 aagtcttcac gaagctggac gcggtgtgca aaacgggcgc gattctggcc tcgaacacct
    14881 cctacctcaa catcgacgaa atcgccgcca tcacaaagag gccgcaggat gtcatgggca
    14941 tgcacttctt cagcccggcc aatgtgatgc gtcttctgga aaacgtccgc ggcgcgaaga
    15001 cctcacccga ggtttacgcc acggccatga aggtcggaaa aaagatcggc aaggtgccgg
    15061 tcctggtcgg cgtctgcgac ggattcgtcg gaaaccggat gctggccaag cgcacccgcg
    15121 aatgcggctt catgctggaa gaaggagcgc ttccctggca gatcgaccgc gtcatttata
    15181 atttcggctt cccgatgggc ccctacgcga tgggcgacat ggcgggcctc gacatcggct
    15241 ggcgaaaccg caaggccaag ttcgactcgc tttcgccgcg cgaacaaaag aacaacatcc
    15301 tggacaaaat ctgcgaaatg ggacgctacg ggcagaaaac gggttcgggc ttctacaagt
    15361 atgacgagaa gcgcaacgcg acgcccgatc cgatgattga agagctgatc atcaaccact
    15421 cgaaggaagt cggcatcgcg cgtcggacca tcacggatca ggaaatcatc gaacgcgcga
    15481 tttactccat gatcaacgaa ggcgcgaaga tcctggagga agggatcgcc gcgcggcccc
    15541 tggacatcga tgtggtctgg ctctacggct acggctttcc ggtgtacctg ggcggcccga
    15601 tgttttacgc cgatacgatc ggactcggca aggtttacga cgcagtcctg aagtaccggg
    15661 atctggtggg cgccgaatac tggacgcccg cgccattgct ggaaaagctg gccaaagaag
    15721 gaaaaggatt ttactccaaa taaaattgat gaactcgtaa aaagcaaccc acaccgtcac
    15781 cccggcgaaa gccggggtcc ataactcatt gtaattactg gatactttcc ttagccggta
    15841 tgacgaaaag ggtgcaaaaa agacttttta ccagaccgtc aaaattcgaa agggaaaata
    15901 gggaggggac gatgaagtat caggaggttt acgcgcgttc gatttttgag cccgccacct
    15961 tttggggcaa agccggggag gccattgagt ggacgaagaa gtgggacaaa gtgc
//
LOCUS       DAOSRM010000047        15936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3774_length_15936_cov_103.119577, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000047 DAOSRM010000000
VERSION     DAOSRM010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15936
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3774_length_15936_cov_103.119577"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..2439)
                     /locus_tag="PLA58_06045"
     CDS             complement(97..2439)
                     /locus_tag="PLA58_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR62393.1"
                     /translation="MERRTDKKDRGAGTDGTLRERAAILESILNGVSLLISYIDKEER
                     YVFVNRAYAWWHDIRPEEAIGKRVSEILKPEEYARISANIKKALDGQRVTYENQVTDR
                     EGRERTLRVHYEPHCGGGEVKGFFSSLLDVTEGRRAEAALQESEQRFRILTESSPTAV
                     MLYQNDKWIYANPAAAEITGYSTTELLSMNFWDFVHPDDRHIVMERGRKRQSGESVTP
                     RYQFKIVCKDRAVKWVDLSGETIVFNGKPAGIISVMDITEQKCAAEEMKERERALATM
                     ISNLPGFVYRCANDHEWTMTFISEQCADVTGFPASDFLSNKITYNEIVRPDYREPLRE
                     KRQETIRRKDFFEEEYPIVAASGETRWVLERGRGVFDPAGDLLFLEGFIGDITARRRE
                     EQEKEKLQAQLTQSQKMESVGRLAGGIAHDFNNMLAVILGRVDLGMTRVEPAQPLYKD
                     LQEIRKAAERSANLTRQLLAFARKQTVSLKVLDLNETVEGMLKILRRLIGEDIEIAWL
                     PGKNVAPVRMDPGQIDQILANLCVNAKDAIGGVGRITIETEITVLDENYCADHKGFIA
                     GDYVTLSVSDNGCGMDKSTLDKLFEPFFTTKGVGQGTGLGLSTVYGIVRQHNGFINVY
                     SEPGRGSTFKIYLPRHPEALAEPTGKESAAAVTGNETILLVEDEPAILEMTGMMLHRL
                     GYTVLSAGKPAEAIELARNHTGEIHLLITDVVMPEMNGRDLAQSIASLHPNIRKLFMS
                     GYPADVIARQGVLDEGVLFIQKPFLMKDLAAKLREAIGNH"
     gene            complement(2445..2642)
                     /locus_tag="PLA58_06050"
     CDS             complement(2445..2642)
                     /locus_tag="PLA58_06050"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOR62394.1"
                     /translation="MDRNVGTIDRIVRLVIAVLLIIANIAGWISGIAGIVLAVLAGML
                     LSSVISGHCPLYGKLGIRKTF"
     gene            complement(2681..3175)
                     /locus_tag="PLA58_06055"
     CDS             complement(2681..3175)
                     /locus_tag="PLA58_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOR62395.1"
                     /translation="MFSAGIITVSDKGSQGKREDKSGPVIAELLAGAAVSIKRTMIIP
                     DEIDQIAQAIVQLADVERLDLILTTGGTGVSPRDLTPDATLKVLDREIPGMAEAMRAS
                     SMKVTPHAMISRAVAGIRGRSLIINLPGSPRGAKENLSVVMPALAHTMEKIQGDERDC
                     ATLL"
     gene            complement(3176..5257)
                     /gene="fusA"
                     /locus_tag="PLA58_06060"
     CDS             complement(3176..5257)
                     /gene="fusA"
                     /locus_tag="PLA58_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR62396.1"
                     /translation="MAKYESKQLRNLAVIGHGGTGKTSLCESLLYVSGKNERLGRVDD
                     GSSTMDYEPEEQKKHVSISSAANFFEWDKHKINIIDTPGDSNFTFDIKCSLSVVDNAV
                     VVIDSVGGVEFQTQKVWEIADEYSLPRLIFINRMDRERADFNKVLESIKTKLKKKGTP
                     VCLPIGAEDKFQGIVDLIAMKAYTFADNKGAGKAVDIPSDLAQEAGALRSSMVEDIAE
                     ADDELMNKYLEAGELSAEELKSGLRKGVLSGGIVPVICGSAVKGIGVTLLADLAASSF
                     ASPVDRGPVKGKKPGTDTVEERQPSEDAPFSAIVFKTIADPYAGRLTLFRVYSGKLNS
                     DSAVYNSTRKITEKFGHIFFLEGKNQKQAEALIPGDIAGVAKLKETVTGDTLCNEKYP
                     IIFEKVAVPPPIMSFAVEPKSRGDEDKIASSIHRLTEEDPTIVFSRNAQTKEMILSGM
                     GQVHIEVNIEKMRRKFGVEVNLNTPKVPYLETIKGKTNVQGRYKKQSGGRGQFGDCTI
                     DIEPLPRGAGYEFVDKIVGGVIPAQYRPAVDKGIQEAAAKGVIAGYPVVDFRISLVFG
                     SYHTVDSSEMAFKIAGSMAFQKGVMECQPILLEPIVNISIEVPDEYMGDVIGDLNSRR
                     GRVLGMDTNGSNQVIKGQVPLAEILKYAPDLRSMTSGRGNFTYTDSHYEEVPSYIADK
                     IIAESKKEAEG"
     gene            complement(5424..7121)
                     /locus_tag="PLA58_06065"
     CDS             complement(5424..7121)
                     /locus_tag="PLA58_06065"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62397.1"
                     /translation="MQSTVWILLKPRLLSARNGLKPNEQNGNRSRLVLFSLIGVTFWA
                     GAFAIFYRVLRYFQNVQDFGDILAMKLLSMMIITFFTLLLFSSIINSLSHMYLSRDLS
                     LLHSMPVGVGDTFLSRWLGSTFDSSWMVATFSLPIFLSYGLTYQAGLAFYPIALCAVV
                     CLCLLASAASSLLVLLGAAVLPAGKIRTLFVILGVGLALLLVLVLRLIRPEQLVNPDS
                     FASVVLYLNSMQALDWPWLPTTWITDAVRLALKREWGPSLFNLALAASCFFSLMFFTQ
                     TAAGVLYFRGFSRAQTTSKRLFTPVKFHGYSWETLLNFLSRPAKAFAVKEIRSFFRDS
                     TQWPQLFLICALIVVYLYNFSVLPLDKSPMKTIYLQNVFSFLNVGLAAFVLSAIAARF
                     VFPAVSMEGEAFWIIQSAPVSMKTFLWIKFFLYCIPLLILSEVLIIASNLLLGVTPFM
                     MFLSIVTVFFLVPAIVGLSVGLGARYPNFKSENPALTATSFGGVLFMLLSFGLIALVI
                     MLEAGPVYRIFMSRMHGHGFSLPQIIWFALSFGLALFLCVFAVFYPMMLGEKNLLKR"
     gene            complement(7126..7851)
                     /locus_tag="PLA58_06070"
     CDS             complement(7126..7851)
                     /locus_tag="PLA58_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR62398.1"
                     /translation="MIKLINLTKDFKTTLAVNRLNLEVAAGEIYGFIGPNGAGKTTTI
                     RMMGGIIEPTDGKILIGGIDMSRDPVGAKKIIGFVPDRPFLYEKLTGMEFLKFIADLY
                     RVDLRVFSRRAPELLERFALWQWKDEIVEAYSHGMKQRLIIAAALLHDPRVMVIDEPM
                     VGLDPEAVRMVRDILKGLARQNVTVFVSTHTLSLAEDLCDRIGVIHKGTLLAQGTVAE
                     LNLAAKTGEARLEEVFLTLVREL"
     gene            complement(7844..9313)
                     /locus_tag="PLA58_06075"
     CDS             complement(7844..9313)
                     /locus_tag="PLA58_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOR62399.1"
                     /translation="MTKIKWHWIWGIGITVVFLGLLAHRLEFFREKPAAAPGPQTVQI
                     TRSPESWMNIYQNRKKIGVIHRTFTGLENGRFQTRESVAMQINTMGVVQALNLSTESE
                     LNPDMTFSSFHFEINSSLFRFAARGYAGQDRLIVFAGLPHAQKKTVLPLKDLPHISGN
                     IYEAAFRGGLEKDQTRSFSIFDPSTLALRSVKVTRFADEVIPIMGKRILTQKFCADFM
                     GAQNCAWLAKDGEILKETGMLGLSMEKVDAQKAREGISAEGIADFSQMASIASNRILP
                     EPSTLTQIVIRVGGVHNLPLMLSGGRQKFSSGVLTITQEETPDTAQPNNGPKLFQQYL
                     QPSPLVQSDAAEIQKQAAKIVSPKDSPPARLRKIVHWVYRNVEKKPVLSVPNALEVLK
                     NKAGDCNEHAVLTAALLRAADVPVQMETGLVYLKGRFYYHAWNTAYVGRWVTADAVFD
                     QIPADVTHIRLARGEGAEQLDLLGVMGKITLEVLSYKND"
     gene            9457..9900
                     /locus_tag="PLA58_06080"
     CDS             9457..9900
                     /locus_tag="PLA58_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOR62400.1"
                     /translation="MKKRGLIIFGGLCLLLSSCAGWMMEEPSLQLRRVSLHPRSLTEI
                     YLVLDLDVQNPNRFDLTIQSFQYKLFLNEREIGSGRLEKEVRIPADSAVRVQAPVVAG
                     FKNWNESLRAVIADKGIPYRIEGEANVRTVFGSRNYPFSKRGKID"
     gene            9897..10763
                     /locus_tag="PLA58_06085"
     CDS             9897..10763
                     /locus_tag="PLA58_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HOR62401.1"
                     /translation="MKRSEGKIRTVNGSLWYRVHGKDGKKTPILVLHGGPGFLSMDDG
                     LEPLWKDRPVFFYDQLGCGKSDRAANRNFYSVENYVAELAEVRAALKLDDVYLMGFSW
                     GCALACAYLLYARPSGVKGVMLCAPYLSSPAWDADQRRNIARLPKGIREAIEEGEKTG
                     NFGEAYESAMMAYYDRHVCLLKPWPESLQKAFSGLNPDVYNTMWGKSEFTIAGALKDF
                     DLTPRLGEIREPVLLTCGDRDEAGVKTVKDFQQLFPDARMAVIPKASHLHQIERPKIF
                     AAVVGDFLQDVD"
     gene            10787..11266
                     /locus_tag="PLA58_06090"
     CDS             10787..11266
                     /locus_tag="PLA58_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62402.1"
                     /translation="MKIRQCFMGVFLLLFCAVLTGMSPVECRGKSSEAAPVQMEWKGN
                     ISNQKEPFIKIVETREAWNDLWLSAFEKPAPDIDFDRCVVACVFLGHQADWLYSIGFA
                     EPVPRGDVWVISYGLAEIVLDLAKPFKASGQYAMKVFSRHKDAKMILEESPESARRR"
     gene            11266..11718
                     /locus_tag="PLA58_06095"
     CDS             11266..11718
                     /locus_tag="PLA58_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62403.1"
                     /translation="MQAREMAATSVGRNALTAGLLCHASAILVGMMTASGADVFFKIV
                     YPFSWVLMTLGAYAELRLRSGRPLKSWRFYGAAMASVFPLLGPPAVFGLIYTCPEEGR
                     EGQAGLSGFVAALLRLRANLLLLFGLSMLLFLLFAVVTGADDPYFRRK"
     gene            11746..15930
                     /locus_tag="PLA58_06100"
     CDS             11746..15930
                     /locus_tag="PLA58_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62404.1"
                     /translation="MRTNKTALQTWVMMVGLIALLIVVPAMLWAADPSAPCDNGPKTE
                     IFIQTGHAGSVNTVEWSPDGRFILSGSSDGGIKLWDAPSGRLIRTFVEGEDVRYVQFL
                     PGGKTFFSVNEKGGVGVWNIAGGRPVRGFSLSREKEPALIDPVSFDGSVVRAIVSSRL
                     YAAQLSEGKALPPEQLPEGAPVKKYFSGYDFGHKNLVTADGKRMLSAVPEDPSDIRFK
                     KLILWELSTGKILFSLDGHSDRIDAVALSLDESYAASGGRDKTVRVWDLKTGKAVAVL
                     TGHQKEIEALAFSPDRKYALSGSRDHAMKLWDIEAGKEVRSFAHPARVTLVRFSPDSR
                     FAVSGDAEGVIRIWDARTGEETMALKTHADSGYVLVMSADGRHVLSGTPDGKIRLWDK
                     TTGKLLESIPAHQEKITAVHFTPDGKHALSASEDKTLKLWDLATGAMQKTFVGHKEKI
                     SRAAVSPDGQNILSLAGTADVRLWNLTSGAERKAFSFPGRVLSIGFADDGRHLLIAYE
                     DKILDHRIIKVLTPEGREVRKYMSAGFHFSEDGKYFLANEYWELTDGGRRAFSPSTGP
                     KKQKLYLDIAKKSSLVDIAGGGILGRFGQSGAFLSLSACAEEGIVLTKSRYDRNIVLW
                     DIRTGLEIRRFAAGHGLLTYDGRKIVAPSGNTLQAFDRASGAPLAPFTGRASMEISAL
                     ALSPDGSVAVTGDGAGEVQLWDIGAGTLKKSLPAQEKEMILATAVSFDRRFAASLARS
                     GVIRIWNMNDGRKVSEVKTEYKPIYAEELAAGDFVDYGSGILAFSPDGLRLACGSGVW
                     EAATGKNLLTFAQPNVPYPWVVFSPDGRFLLSRNMTWDASSGRLEQTLECVKNAAKSF
                     YSADGRLILSADLEGGFYVMDAVTGRLIRKFADFVPPGPFDVSHDKKMMIAVEKDQNK
                     LTVWDLTSGKKNAAIAVDQNILGVLLTSDRRRAILKHGIAISEYDLPAGKELVQLVSF
                     TDGGWIVMTPEGYYNASSRGDRYINVRIGESVYGIENYRESFFRPEAVRMALSGDSLK
                     DFRKLTDVKEPPIVRIVDTPASVSADEVHVRLNLHEQGGGIGDIHLYLNGTAVVLDTR
                     SVSEPEKSQQAVFRAYPLKLVRGRNIIHATALNGDNNMRSNEAEWEVTASYAAAAGPS
                     LAALAIGVGGYGDPGLKLPYAAAGAELVAGAFKGAGEGIFAKAEIKALTRPEETTAEA
                     ILREMKAFQTLRAEDLFVFYISGFAVVEEGEYFLVTSNVNSLSAEKLKTDAIALRKLQ
                     EALGNIPAAKKLILIDACKPGADGQTAAQTKGMSEETALKIFGGTLGSTVLSSSVPLP
                     EALEGYQGHSLFAHLVTEGLKGGADAEKTGFIKTTDLTRYMRNEMPALSEKIFKQIRY
                     PVVSINGQAFPVGKVK"
BASE COUNT         3456 a         4308 c         4611 g         3561 t
ORIGIN      
        1 aagatttctc gtcgctcacg ctcctcgaaa tgacattttt caaagctctc ccatcaaggg
       61 tggggaggaa cggaagattc ttaaaaagag gtttttttaa tggtttccga tggcttcgcg
      121 cagtttggcc gccagatctt tcatcaaaaa cggcttctga atgaaaagca ccccctcgtc
      181 cagaacgccc tggcgcgcga tgacatcggc ggggtacccg gacataaaaa gtttccggat
      241 attcggatgg agggacgcaa tgctttgggc caggtcccgt ccgttcattt cgggcatgac
      301 cacgtcggtg atcagaagat gaatttcgcc ggtgtggttg cgggccagtt caatcgcttc
      361 cgccggtttg ccggctgaca acacggtgta gccgaggcga tggagcatca ttcctgtcat
      421 ttccaggatc gcaggttcgt cctcaaccag caagatggtt tcatttccgg tgacggctgc
      481 cgcgctttcc ttcccggtcg gttccgccaa ggcttcagga tgccggggca ggtagatttt
      541 gaaggtcgat ccccggcccg gttcgctata gacgttgata aagccgttgt gttgcctgac
      601 gatcccgtac acggtggaca acccaaggcc ggtgccctgg cccacaccct tggtcgtaaa
      661 gaaaggttca aacaatttat ccagcgttga tttatccatg ccgcagccgt tgtcgctgac
      721 cgagagcgtc acatagtcac cggcaatgaa ccctttgtga tcggcgcagt agttttcatc
      781 gagtacggtg atttccgttt cgatagtgat cctgccgacg cccccgattg cgtccttggc
      841 gttgacgcaa aggttggcga ggatctgatc aatctggccg gggtccatcc tgaccggcgc
      901 cacatttttg cccggcagcc aggcgatctc gatgtcctcg ccgatgagcc gccggagaat
      961 tttcagcatc ccttcgacgg tctcgttcag atcgagcact ttcagcgaaa cggtctgctt
     1021 gcgcgcgaag gccagcaact gacgagtgag attggccgac cgttcggcgg ctttccggat
     1081 ttcctgcaag tccttataca gaggttgggc cggttccacc cgcgtcatgc ctaagtccac
     1141 gcgacccagg atgacggcga gcatattgtt gaaatcatgg gcaatgcctc cggcaagacg
     1201 tcccacggat tccatcttct gcgactgggt caactgtgcc tgcagctttt ccttttcctg
     1261 ctcttcacgc cggcgagccg tgatgtctcc gatgaaacct tccagaaaga gcagatcccc
     1321 cgccggatcg aaaacgccgc gtccgcgttc cagtacccat cgggtttctc cggaagcggc
     1381 gacgatcgga tactcttctt cgaagaaatc ttttcgccgg atcgtctcct gccgcttttc
     1441 tctcaagggt tcgcggtaat ccggacgaac gatctcgttg taagtgattt tgttgctcag
     1501 gaaatcggat gctggaaagc cggtgacgtc ggcgcactgt tcgctgatga acgtcatcgt
     1561 ccattcgtgg tcattcgcgc agcggtaaac gaatccggga agattgctga tcatcgtggc
     1621 cagcgcccgc tcccgttctt tcatctcttc ggccgcgcat ttttgctcgg tgatgtccat
     1681 taccgaaata atgccggcgg gctttccgtt gaacacgatg gtttcgcccg agaggtcgac
     1741 ccatttgacc gcccggtctt tacagacaat tttaaactgg tagcgaggag tcacggattc
     1801 gccgctctgc cgtttccggc cccgctccat gacgatatgc cggtcgtcgg ggtggacaaa
     1861 gtcccagaaa ttcatggaga ggagttccgt ggtgctgtaa ccggtgatct ccgccgccgc
     1921 gggattcgcg tagatccatt tgtcgttctg gtagagcatg acggctgtcg gagaggattc
     1981 cgtgaggatt cggaatcgtt gttcgctctc ctgcagggcg gcctccgccc gccggccttc
     2041 ggtgacgtcc aggagggatg aaaagaatcc ttttacctcc ccgccgccgc aatgcggttc
     2101 ataatgcacg cggagggttc tttcccggcc ttcccggtcg gtgacttgat tttcataagt
     2161 tacccgctgc ccgtcgagag cctttttgat gtttgcggag atcctggcat actcctccgg
     2221 ttttaaaatt tcggaaactc tttttccgat cgcctcttcc ggccggatgt catgccacca
     2281 cgcgtaggcg cggttcacaa aaacgtatcg ctcctctttg tcgatgtaag aaatgagcag
     2341 cgacacgccg ttcaggatgc tctcgagtat cgcggcccgc tcccgcagag tgccgtcggt
     2401 gccggcgccc cggtcttttt tgtcggttcg gcgttccatt tttgtcagaa ggtttttcta
     2461 attcccagtt taccataaag agggcagtgt cccgatatca cgctggagag cagcatgccg
     2521 gcaaggacgg caagaacaat gcctgcgatt ccgctgatcc accccgcgat attggcgatg
     2581 atcaaaagaa cggcaatgac gagacgaacg atcctgtcga tggtccccac gtttcgatcc
     2641 attggctctc ctccttataa aaatgaagtg gttgggtgat ctacagcaat gtcgcgcaat
     2701 ccctctcgtc gccctgaatt ttctccatgg tgtgcgcaag cgcgggcata acgacggaaa
     2761 gattttcttt tgcgcctctg gggctgccgg gcaggttgat gatcagggat cgtccgcgga
     2821 tgccggcgac agcgcgggag atcatggcgt gcggggtgac tttcatgctg gaagccctca
     2881 tggcttcggc cattccggga atttccctgt ccagaacttt cagcgtggcg tccggcgtca
     2941 ggtcgcgcgg gcttacgccc gtgccgccgg tcgtcaaaat caggtccagt ctttcaacat
     3001 ccgccaattg aacaatggct tgggcaattt gatcgatttc gtcggggatg atcatggttc
     3061 tcttgatgga gacagcggcg cccgccagca gttcggcgat gacggggccg cttttatctt
     3121 cccgtttacc ctgtgagccc ttgtcgctca ccgtgatgat tccggcagag aacatttaac
     3181 cttccgcttc tttttttgat tcggcgatga tcttgtccgc gatgtaggag gggacttctt
     3241 catagtgcga atcggtatac gtgaagttgc cccgcccgct ggtcatggaa cgaagatccg
     3301 gcgcgtactt caggatttcc gcgagcggca cctggccttt gatcacctgg ttggagccgt
     3361 tggtgtccat acccagcacg cgcccgcggc ggctgttgag atcgccgatg acgtcgccca
     3421 tgtattcatc gggcacttca atggaaatgt tgacaatcgg ttccagaaga atcggctggc
     3481 actccatgac gcctttctga aaagccatgg acccagcgat tttaaaggcc atttccgaag
     3541 aatcgacggt atggtaggaa ccaaaaacca gagagatgcg aaaatccacg acggggtagc
     3601 cggcgataac gcctttggcc gccgcttcct gaatcccttt gtcaaccgca gggcggtatt
     3661 gggcgggaat aacgccgccg acgattttat ccacgaactc atagcccgcg ccgcgcggca
     3721 gcggctcgat atcgatcgtg caatcgccga actgaccgcg tccgccggac tgcttcttgt
     3781 aacgaccctg aacgttggtc ttgcctttga tggtttccag ataggggact ttcggcgtgt
     3841 tcagattgac ttcaacgccg aattttcttc tcattttttc aatgttgact tcaatatgca
     3901 cctggcccat tccggagaga atcatctcct tggtctgggc attgcgggag aagacgatgg
     3961 tcgggtcttc ttccgtcagg cggtgaatcg aggaagcgat tttatcctca tccccccggg
     4021 atttcggctc cacggcaaaa gacatgatcg gcggcggaac ggcgactttt tcaaaaataa
     4081 tggggtattt ctcgttgcac aacgtgtctc ccgtgacggt ttcctttaat ttggcgactc
     4141 cggcgatatc gcccgggatc agcgcttccg cctgtttctg gttttttcct tccaaaaaga
     4201 aaatatgacc gaatttttcc gtgatttttc tcgtggaatt atacacggcc gaatcggaat
     4261 tgagctttcc ggaatagacc cggaacagcg tcagcctgcc cgcgtaggga tcggcgatgg
     4321 ttttgaaaac aatggccgag aaaggggcgt cttccgacgg ttgcctttct tcaaccgtat
     4381 ccgtaccggg ctttttgcct ttcacggggc cccggtcaac gggagaggca aaggaactgg
     4441 cggccagatc cgcgagcagg gtaacgccga tgcctttgac ggctgaaccg caaatcaccg
     4501 gaacgatgcc gcccgacaac acaccctttc ttaagccgga tttgagctcc tcggcggaaa
     4561 gctcgccggc ttccaggtat ttattcatca attcatcgtc ggcttcggca atgtcttcca
     4621 ccatcgatga tcgaagcgcg cctgcttcct gggccagatc ggatggaata tccacggctt
     4681 ttcccgcgcc tttgttgtcg gcgaaggtgt aagccttcat ggcgatcaga tccacgatgc
     4741 cctgaaactt gtcttccgcg ccgatgggca ggcagaccgg ggtgcctttc tttttcagtt
     4801 tggttttgat gctttccagc accttgttga aatcggctct ttcgcggtcc atgcgattga
     4861 tgaaaatcag gcggggcagg gaatattcgt cagcgatttc ccatactttc tgcgtctgga
     4921 attccacgcc tcccacggag tcgatgacca cgaccgcatt gtccacaacg ctcagggaac
     4981 acttgatgtc aaacgtgaaa ttggaatcgc ccggagtgtc gatgatattg attttatgct
     5041 tgtcccattc gaaaaagttg gccgccgacg agattgaaac atgttttttc tgctcttcag
     5101 gctcatagtc catggtggaa gagccgtcgt ccacacggcc cagccgctcg ttttttccgg
     5161 aaacatacag aagggattca caaagagagg ttttgcccgt gccgccgtgg ccgatgacgg
     5221 ccagatttct aagttgtttg gattcgtact ttgccatgaa aactcctttc cattatctca
     5281 acaggatcag caaaagatct gatgaaacgg gtcattgcag aatgcgcgct gtattagcgt
     5341 atccggcaag taaaatcaag ataattttca acttctggcg atttcaggga gcttctctgg
     5401 cctgcgcaaa gaccaatggg aaatcatctt ttcagaagat ttttttcgcc cagcatcatg
     5461 ggataaaaga ctgcaaatac gcacagaaac agcgcaagtc caaaggacag ggcaaaccag
     5521 ataatctgcg gcagggaaaa gccgtggccg tgcattctgg acatgaagat tcgataaacc
     5581 ggccccgcct ccagcatgat gaccagggca atcaggccga aggagagcag catgaacagg
     5641 acgccgccga agctggtggc tgtaagcgcc ggattttcag acttgaagtt cgggtagcgg
     5701 gcgcccaggc ccacggacag tccgacgatg gccgggacca gaaagaagac ggtgacgatg
     5761 gacaggaaca tcatgaaagg cgtcacgccg aggaggagat tggaagcgat gatcaggact
     5821 tccgaaagaa tcaaaagcgg gatgcagtag agaaaaaact tgatccagag aaaggttttc
     5881 atggaaaccg gcgcggattg aatgatccag aaagcttctc cttccatgct gaccgcggga
     5941 aaaacgaagc gtgcggcaat ggccgaaagc acgaaagcgg ccagccccac attcagaaaa
     6001 gaaaacacat tctggagata aatcgttttc atgggcgatt tatccagggg caggacggag
     6061 aaattgtaga gatagacgac gatcagggcg cagatcaaaa agagctgcgg ccactgcgtc
     6121 gaatcccgga agaaggagcg gatttccttg acggcgaagg ctttcgcggg ccgtgaaaga
     6181 aaattcagaa gcgtttccca actgtatccg tgaaatttca cgggcgtaaa aagccgtttg
     6241 gacgttgtct gagcgcggga aaacccccga aaatagagga cgcccgcggc ggtttgggta
     6301 aaaaacatca atgaaaaaaa gcagcttgcc gcaagggcca ggttgaaaag cgacggcccc
     6361 cattcccttt tcagggccag gcgcaccgca tccgtgatcc aggtggtggg gagccatggc
     6421 cagtccagcg cctgcatgga gttcaggtac agaacgaccg aagcaaagct gtccgggttg
     6481 accagctgct ccggacgaat caggcgcaga accagcacga ggagcagcgc cagacctacg
     6541 cccaggatga cgaaaagggt tcggattttt ccggcgggaa gaaccgccgc gcccagaagc
     6601 accagcaggc tgcttgccgc ggaagccagc aggcacagac agacgacggc gcaaagcgcg
     6661 atggggtaaa aggcaaggcc ggcctgatag gtgaggccgt aggacaggaa aatgggaagg
     6721 ctgaaggtcg ccaccatcca ggaagaatcg aacgtgctgc ccagccagcg cgacaggaac
     6781 gtatccccga cgccgaccgg catggaatgc agcagggaca ggtcccggct taaatacatg
     6841 tgcgacaggc tgttgatgat gctgctgaac agcaaaaggg tgaagaaggt gatgatcatc
     6901 atggacaaaa gcttcatggc caggatgtcg ccgaaatcct gaacgttttg aaaatatctc
     6961 aggacgcggt aaaaaatggc aaaggcaccg gcccagaacg tgacgccgat cagggaaaaa
     7021 aggaccagtc gcgaccggtt cccgttttgt tcattcggtt tcagtccgtt tcgggcggac
     7081 agcagtctcg gtttcagcaa tatccatacg gtgctttgca taggcttaca attcccggac
     7141 cagcgtcaga aagacttctt ccaggcgggc ttcgccggtt ttggcggcca gattcagctc
     7201 ggcgaccgtt ccctgcgcga gaagcgttcc cttgtgaatc acgccgatcc ggtcgcacaa
     7261 gtcctcagcc aggctgagcg tgtgcgtgga cacgaaaacc gtcacgtttt gccgggccag
     7321 ccccttcagg atatccctga ccattcgcac cgcttccggg tccagcccga ccatcggttc
     7381 gtcgatgacc atcacccgcg ggtcgtgaag cagcgccgcg gcaatgatca atcgctgctt
     7441 catgccgtgg gaatacgcct cgacgatttc gtctttccat tgccagagag caaaccgttc
     7501 cagcagttcc ggggctctgc gggagaaaac ccggagatcg acgcggtaca aatccgcgat
     7561 gaatttcaaa aactccatgc cggtaagctt ttcgtacaaa aacggcctgt ccggaacaaa
     7621 accgatgatc tttttggccc caacgggatc ccggctcatg tcgatcccgc cgatgagaat
     7681 tttcccgtcg gtcggttcga tgatgccgcc catcatgcgg atggttgtgg ttttgcccgc
     7741 gccgttgggg ccgatgaagc cgtaaatttc tccggcggcc acctccaggt tcaggcggtt
     7801 gacggccagc gtcgttttaa aatccttggt caggttgatc agtttaatca ttcttgtaag
     7861 agagaacctc cagggttatt ttgcccatga cgcccagcag gtccagctgc tcggcgcctt
     7921 cgccgcgcgc caggcgaata tgcgtgacgt ccgccggaat ctggtcaaaa acggcgtcgg
     7981 ctgtcaccca ccggcccacg taagccgtgt tccaggcgtg gtaataaaaa cggcctttca
     8041 aatacaccag accggtttcc atttgaacgg gaacgtctgc agcccgaagc agcgcagcgg
     8101 tgagcaccgc gtgctcattg cagtcacccg ccttgttttt caaaacttcc agggcgttgg
     8161 ggacggacag gaccggcttc ttttcgacgt tgcggtaaac ccagtgcaca attttcctga
     8221 gcctggcggg tgggctgtct ttcggagaaa cgattttggc cgcctgcttt tgaatttccg
     8281 cggcgtcgga ctgaaccagc ggcgacggct ggagatactg ttgaaagagc ttcgggccgt
     8341 tgttcggctg ggctgtgtcg ggcgtttcct cttgagtgat cgttaaaacg ccggaagaaa
     8401 atttctgcct gccgccggaa agcatcaggg gcaggttgtg aacgccgcca acccgaatga
     8461 cgatctgtgt caatgtggaa ggttcgggca gaatccggtt tgaggcgatg gaagccattt
     8521 gcgaaaagtc ggcaatgcct tccgccgaga tgccttcgcg cgccttttgg gcatccacct
     8581 tttccatgga cagacccagc atgcccgttt ctttcaggat ttcaccgtct ttggccagcc
     8641 aggcgcagtt ttgggcgccc ataaagtcgg cgcagaattt ttgcgtcaaa atgcgcttgc
     8701 ccatgatcgg gatgacttca tcggcaaagc gcgtgacctt tacgctgcgg agggcaagcg
     8761 ttgacgggtc gaagatgctg aagctgcggg tttggtcctt ttccagcccg ccccgaaagg
     8821 ctgcttcata gatatttccg ctgatatggg gaaggtcctt cagggggaga acggttttct
     8881 tttgggcatg gggcagtccc gcgaagacga tgagcctgtc ctgacctgcg tagccccggg
     8941 cggcaaaacg aaaaagactg gagttgatct caaaatggaa agaggaaaag gtcatgtcgg
     9001 gattgagctc cgattcggtg gacagattca gggcctgcac gacgcccatg gtgttgatct
     9061 gcatggcgac gctttcccgg gtctggaagc ggccgttttc cagcccggtg aaggtgcggt
     9121 ggatcacgcc gatttttttt ctgttctggt agatgttcat ccaggattcc ggcgagcggg
     9181 taatttgaac tgtttgcggc ccgggcgccg cggccggttt ttcccggaag aactccaggc
     9241 gatgagccag caggccgaga aataccaccg tgatgccgat gccccagatc caatgccact
     9301 tgatttttgt catgcgcgcc aaactttccc agccgtcaca gccggcaaaa tacgacgtca
     9361 gtatatagac aggaaccggc cgggtcaact atcagtattg gaccggtatt ggacaatctt
     9421 gttgacagcg ccatccatcc ttcgtatagt ttcaccatga aaaaacgagg tctgatcatt
     9481 ttcggaggtt tgtgcctgct gctttcgtcc tgtgccggct ggatgatgga agaaccttcc
     9541 ttgcagcttc gccgggtttc gcttcatccc cgctccctga cggagattta tctggtcctc
     9601 gatctggacg tgcagaatcc gaaccgattc gatctgacga tccagtcttt tcagtacaag
     9661 cttttcttaa acgagcgtga aatcggaagc ggacgcctgg aaaaggaagt ccggattccc
     9721 gccgattccg ccgtccgggt tcaggcgccg gtcgtggccg gcttcaaaaa ctggaacgaa
     9781 agccttcggg ccgtgatcgc cgacaagggc attccctacc gcattgaagg agaggcgaac
     9841 gtcaggacgg ttttcggaag caggaactac cctttttcca aaagaggaaa gatcgattga
     9901 agagaagtga aggaaagatt cgcacggtca acggcagcct ctggtacagg gttcacggga
     9961 aagacgggaa gaaaacgcct atcctcgtcc tccacggcgg ccccggcttt ttaagcatgg
    10021 atgacggtct cgagccgttg tggaaagacc gtccggtgtt tttctacgac cagctgggct
    10081 gcggcaaaag cgaccgggcc gcgaacagaa atttttattc ggtcgagaat tatgttgcgg
    10141 aattggcgga agttcgggcc gccttaaagc ttgacgacgt ttacctgatg ggattttcct
    10201 ggggatgcgc gctggcctgt gcctacctgc tgtacgcgag gccttccggt gtaaagggcg
    10261 tgatgctctg cgcgccgtat ctttcttctc cggcctggga tgccgaccag cgccggaata
    10321 tcgcccggct gccgaaaggc attcgggaag ccattgagga aggtgaaaaa acgggcaact
    10381 tcggcgaggc ttacgaatcg gccatgatgg cctactacga caggcacgtc tgtctgctga
    10441 aaccctggcc cgaatcgctt cagaaggctt tttccggcct caatccggat gtgtacaata
    10501 cgatgtgggg caaaagcgaa tttacgatcg ccggagcgct caaggacttt gatctgacgc
    10561 cgaggctcgg ggaaatccgc gagcccgtcc tgctcacctg cggggaccgg gacgaggcgg
    10621 gcgtcaaaac ggtgaaggat tttcagcagc tctttcccga cgcccgcatg gccgtcattc
    10681 caaaggcatc ccatctgcat cagatcgaac gcccgaaaat ctttgccgcc gtggtcgggg
    10741 atttcctgca ggatgtcgat tgacgcttct tctcggagat tggaacatga aaatccgcca
    10801 gtgttttatg ggcgtcttcc ttttgctctt ttgcgccgtt ctgacgggca tgagcccggt
    10861 tgaatgccgc gggaagtctt cagaagcagc gccggttcag atggaatgga aagggaatat
    10921 ttccaatcaa aaagaaccgt tcatcaaaat cgtcgaaaca cgggaggcat ggaacgatct
    10981 atggctcagc gcctttgaaa aaccggcgcc ggatatcgat tttgatcgct gcgtcgtggc
    11041 ctgcgttttc ctcggacatc aggcggactg gctttactcc atcggatttg cagagcctgt
    11101 cccgcgcggc gatgtctggg tcatctccta cggcctggcg gaaatcgtcc tcgatctcgc
    11161 caaacccttt aaggcctccg gacaatatgc catgaaagta ttttcaaggc acaaagacgc
    11221 caaaatgatt ctggaagaat ctcccgaatc agcccggaga agataatgca ggcaagggaa
    11281 atggccgcca cctctgtcgg aaggaatgcc ctgaccgcgg gattgctctg tcatgcctcg
    11341 gcgattctcg tcggcatgat gacggcatcc ggggcggatg tttttttcaa gatcgtttat
    11401 ccgttttcct gggttttgat gacgctcggg gcttacgccg aattgcgcct ccgaagcgga
    11461 cggcccctga agtcctggcg attctacggc gcggcaatgg cgtccgtatt tccgctgctg
    11521 gggccgccgg cagtttttgg cctgatctac acctgcccgg aagaaggcag ggaagggcag
    11581 gccggtctgt cgggatttgt tgcggcgcta ctcaggcttc gggccaacct gcttcttctt
    11641 tttggtttga gcatgcttct ttttctgctg ttcgccgttg tgaccggcgc ggatgatccg
    11701 tatttcagaa gaaaatgaaa agcgggagtt tccatgaaag cttctatgag aacgaacaaa
    11761 acagcattgc agacgtgggt gatgatggtg ggtctgatcg cgctgctgat cgttgtgccc
    11821 gccatgcttt gggcggccga tccttccgcg ccctgcgata acgggccgaa gacggaaatc
    11881 ttcattcaga ccgggcatgc cggttcggtg aatacggtgg aatggtcgcc cgacggaagg
    11941 tttattcttt cgggaagcag cgacggcggc atcaaactgt gggacgcccc aagcggcagg
    12001 ctgattcgaa catttgtgga gggcgaagac gtccgctacg ttcaatttct gcccggcggc
    12061 aagaccttct tttccgtgaa tgagaaaggc ggcgtcgggg tatggaacat tgccggcggc
    12121 cggccagtcc ggggattttc cttaagccgg gaaaaagagc cggcgctcat cgatccggtt
    12181 tcctttgacg gctccgttgt aagggccatc gtttcctcca gactttacgc ggcgcagctt
    12241 tcggagggaa aagcgttgcc cccggagcaa ctgcctgaag gcgcgcccgt caaaaaatat
    12301 ttttccggat acgatttcgg acacaaaaat ctggtgaccg cggacggtaa aaggatgctt
    12361 tccgccgttc ccgaggatcc gtcggatatc cggttcaaaa aactgatcct gtgggagctt
    12421 tccacgggga aaattctttt ttcgctggac ggccacagcg acaggatcga tgccgtggcc
    12481 ctgtcactgg acgagtccta tgccgcgtcg ggcgggcggg acaaaaccgt ccgcgtttgg
    12541 gatttaaaga cggggaaggc cgttgccgtg ttgaccggcc atcagaaaga aatcgaagcg
    12601 ctcgcctttt cacccgaccg gaaatatgcg ctatccggca gccgcgacca tgccatgaag
    12661 ctgtgggaca tcgaagcggg gaaggaagtt cggtcctttg cccatcccgc aagagtcact
    12721 cttgtccgtt tctcgccgga tagccgcttt gccgtctccg gagacgcgga aggcgtcata
    12781 cgcatttggg acgcccggac cggggaagag accatggcgc tcaaaaccca tgccgacagc
    12841 ggctatgtgc tggtgatgtc ggccgacggc cggcatgttc tcagcggaac gccggacggg
    12901 aaaatacgcc tgtgggataa aaccaccgga aaactgctgg aaagcattcc tgcccatcag
    12961 gaaaagatta ccgccgttca tttcaccccg gacggcaagc acgccctgtc ggccagcgag
    13021 gacaaaaccc tgaaactgtg ggatctggca accggcgcaa tgcaaaagac gtttgtcggc
    13081 cacaaagaga aaatatccag ggccgccgtc tcgccggacg gtcaaaatat tctcagtctg
    13141 gcgggaaccg ccgatgtccg gctctggaat ctgacatccg gcgctgagcg gaaagctttt
    13201 tcgtttcccg gacgcgttct ttccatcgga tttgccgacg acggcaggca tttgttgatt
    13261 gcctacgaag ataaaattct ggaccaccgg atcatcaaag tcctgacgcc ggaggggcgt
    13321 gaagtcagga aatacatgag cgccggattt catttttccg aagacgggaa atattttctg
    13381 gccaatgaat actgggagct gaccgacggg gggcggcggg cgtttagccc ctcgaccgga
    13441 ccaaagaaac aaaaactcta tctggacatc gccaaaaaaa gctcgctcgt ggacattgcc
    13501 ggcggcggca ttctgggccg cttcgggcag agcggcgctt ttctttccct ttccgcctgc
    13561 gcggaagaag gcattgttct gaccaaaagc cgctacgacc ggaacatcgt tttgtgggat
    13621 attcggaccg gacttgagat tcggcgcttt gccgccggac acggactgct gacctatgac
    13681 ggaagaaaaa ttgtcgcgcc gtccggcaat accctgcagg cctttgaccg ggcgagcggc
    13741 gcgccgctcg cgccttttac cggcagggca tcgatggaga tcagtgcgct ggcgttgtcg
    13801 ccggacggaa gcgtcgccgt aacgggagat ggggcagggg aggttcagct ctgggacatc
    13861 ggcgccggca cactgaaaaa gagcctgccg gctcaggaga aggaaatgat tctggccacg
    13921 gcggtttctt tcgatcgtcg ttttgcggct tcgcttgccc gctccggcgt tattcgaatc
    13981 tggaatatga acgacggtcg gaaggtgtcc gaagtgaaga cggagtacaa gcccatttac
    14041 gccgaagagc ttgccgccgg agacttcgtc gattatggga gcggaattct ggccttctcc
    14101 cccgacggcc ttcgccttgc ctgcggatcg ggcgtctggg aggccgcaac cggaaaaaat
    14161 ctgctgacct ttgcccaacc caacgtcccc tatccctggg tggttttttc gcctgacggc
    14221 cgtttccttc tttcccgcaa catgacctgg gatgcgtcct ccggccgtct tgaacagacg
    14281 ctggaatgcg taaagaacgc ggcgaaatct ttttattccg cggacggacg gctcattctt
    14341 tccgccgatc tcgaaggagg cttttacgtc atggacgcag tgaccggccg cttgattcgc
    14401 aagtttgccg attttgtgcc gcccgggccg tttgacgttt cgcatgacaa aaaaatgatg
    14461 atcgcggtgg aaaaagatca aaacaaactg acggtgtggg atctgacgtc cggaaagaaa
    14521 aatgcggcca tagcggttga ccagaacatc ctgggcgttt tgctgacgtc cgacaggcgc
    14581 agagctatcc tgaagcacgg cattgccatc agtgaatatg atcttcccgc cggaaaggag
    14641 cttgtgcagc tcgtcagttt caccgacggc ggatggatcg ttatgacgcc cgaagggtat
    14701 tacaatgcct catccagagg cgaccgctac atcaatgtcc gcatcggaga gtcggtttac
    14761 ggcattgaaa actaccgcga atcttttttt cgccccgaag cggtgagaat ggcgctgtcc
    14821 ggggattctt tgaaggattt ccgcaaactg accgatgtga aagaaccgcc catcgtacgg
    14881 atcgtcgata cgcctgcctc cgtaagcgcc gatgaggttc atgtccggct gaacctgcat
    14941 gaacagggcg gcggcatcgg agatatccat ctttacctga acggaaccgc cgtggtcttg
    15001 gatacccgtt cggtttctga gccggaaaag tcgcagcagg ccgttttcag ggcctatccc
    15061 ctgaagcttg tccgcgggag gaatatcatc catgccacag ctctgaacgg cgacaacaac
    15121 atgaggagca acgaggcgga atgggaagtg acggcctcct atgctgctgc ggccgggccg
    15181 tcgcttgcag ctttggccat cggtgtcggc gggtacgggg atcccggact caaactaccg
    15241 tatgcggcgg caggcgcgga gcttgttgca ggcgcgttca aaggggcggg ggaagggatc
    15301 tttgcaaagg cggagatcaa ggccctgacc cggcccgagg agacaacggc ggaggcgatt
    15361 ctccgggaga tgaaagcttt tcaaaccctg cgcgcggagg atctgtttgt tttttacatc
    15421 tccggcttcg cggtcgttga agagggggaa tattttcttg tgacgtccaa tgtgaattcg
    15481 ctaagcgctg aaaaacttaa aacggacgct attgccctgc gaaagctgca ggaggcgctc
    15541 ggcaatattc cggcggctaa gaaactgatc ctgatcgacg cctgcaaacc cggcgctgac
    15601 gggcaaacgg cggctcaaac aaaggggatg agcgaggaga ctgccctgaa gattttcggc
    15661 gggacgctgg ggtcgaccgt tctttcatcc tccgtgcctc ttcccgaagc gctggaaggc
    15721 tatcaggggc acagcctgtt tgctcatctg gtgacggaag gattgaaggg cggggcggac
    15781 gcagaaaaga ccggattcat caaaaccacc gatcttacgc gctacatgcg caacgaaatg
    15841 cccgcgcttt cggagaagat ttttaagcag atccggtatc cggtcgtttc catcaacggc
    15901 caggcgtttc ccgtgggaaa agtaaaatga gcaatc
//
LOCUS       DAOSRM010000048        15725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3845_length_15725_cov_125.004467, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000048 DAOSRM010000000
VERSION     DAOSRM010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15725
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3845_length_15725_cov_125.004467"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..517
                     /locus_tag="PLA58_06105"
     tRNA            444..517
                     /locus_tag="PLA58_06105"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Gln,seq:ctg)
     gene            547..888
                     /locus_tag="PLA58_06110"
     CDS             547..888
                     /locus_tag="PLA58_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOR62405.1"
                     /translation="MKLIIAIVQSDKIKDVKQALRDVNVGKITVSSVVGCGAQVGKTD
                     TRDGVVTNGNLLDRVRFEIAVNDDYVKPTLEAVIRGARTGSIGDGKIFIVPLEECIRI
                     RTGETGRLAIG"
     gene            1085..2617
                     /locus_tag="PLA58_06115"
     CDS             1085..2617
                     /locus_tag="PLA58_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOR62406.1"
                     /translation="MSIHSYEELAALHAISKILAQPMELRDQLEQILHEMSLRLGMQR
                     GMISLLDHEKKEAWLDIAHDVNIDGMEVTYKPGEGITGKVAETGRPMAVANLGQEALF
                     LDRTGARRHLNRAELSFLCVPIIYDASVVGVLSADKVAKQVEDLEKELAMMGSVAELM
                     AKAVHIRALEEENRRLRKIVGQQHAPSMDIIGHSKAIQEVFGLVAQVSDSNTTVLITG
                     ETGTGKELIARAIHNNSPRKGGPLVQVNCAAIPDTLIESELFGHEKGSFTGALQTRRG
                     RFEEANGGTIFLDEVGELSAAAQVKLLRVLQEKRFQPLGSSRVVNVNVRIIAATNRNL
                     EQDILADRFRADLFYRLNVFPIYLPPLRERGSDVILLADHFLLKYSREMGKNVKRIST
                     AAIEAFLSHKWPGNVRELENCIERAVLLTRTDTVDCLHLPPSLHAGEKDPERKDRSKL
                     SSVVEAQERTLIIDALTESGGNQTKAAKILGTTKRIIQYKISKMGIDPKKFSRKKKGS
                     EA"
     gene            2935..4065
                     /locus_tag="PLA58_06120"
     CDS             2935..4065
                     /locus_tag="PLA58_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HOR62407.1"
                     /translation="MCRLFAVTSNNPLSPMMAINALNVMKEGHDGSGVGLFMTDLGGE
                     FEKFKSEPILSGIFSNEGIKALDRFMIDQDFMVKYKLSFRPAKPTPPGTPKRDNYVIR
                     VYEYPAEWESLSREEIDYRLMMVQLQLRQMGEKDQSMMVFSFWPDVIIIKEVGDPLAV
                     AEYLGLDRKDLTARMILSQGRQNTNYSIDIYACHPFFIQGMATMTNGENTAFVPIREF
                     LMSRNFPGYTGYQSDSEVFTHILHYTQNKLGLGMEMYKHVITPLRDEELARHPDGRML
                     RNLKQSCRPLIIDGPNCVIGCLPDKSMFMVQDAKKLRPGVVGGRPGIFAFSSEMCGLD
                     AAIPERDINLDDQPMRYETVIVRRGRQEIEKWNQWDTLPHLH"
     gene            4029..5663
                     /locus_tag="PLA58_06125"
     CDS             4029..5663
                     /locus_tag="PLA58_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOR62408.1"
                     /translation="MEPVGHIAPSTLSAKDLPWQIAWQKDRCTLCGRCTTVCPVRAIE
                     LGVHRKRALTTPVGLESRPANLFSVYHGIDQRTDPAHACVGCGMCTLVCPNNAIVPLR
                     ADETDKLRFHVNQGGEARRRGGRRNAPESVLDQIKFVRISMLTDPALDAGRHEFELRT
                     LLGRILPPERMLQAGRENGWMPPVREIYPMIIGGMSFGALSPNMWEGLQMGVAYLNEE
                     MGMPVRICTGEGGCPPRLLKSRFLKYVILQIASGYFGWDEIIHNLPQMKEDPCAIEIK
                     YGQGAKPGDGGLLMWYKVNRLIAAIRGVPQGISLPSPPTHQTKYSIEEAVAKMIQSMS
                     MAWGFRVPVYPKISATSTTLAVLNNLTRNPYAAGLAIDGEDGGTGAAYNVSMNHMGHP
                     IASNLRDAYLTLVKLGKQNELPLFAGGGIGKNGNLAANAAALIMLGASGVQTGKYIMQ
                     AAAGCLGSEGDRCNVCNVGVCPKGITSQDPRLDRRLDPERVAQRLVDVYLTFDTELKK
                     IVAPLGRSTSLPIGMSDALGISDADAAGRLNIKYVL"
     gene            5727..8063
                     /locus_tag="PLA58_06130"
     CDS             5727..8063
                     /locus_tag="PLA58_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR62409.1"
                     /translation="MTRKDTIKIAGVENTVRVDSRILEERIQKAVADGCRHIEVTALG
                     QHGIGGRLWRAHDEPVKVDVLGTSGQRLGSMGFANTTIQAFGPASDDVGWLNAGAKII
                     VHGNATNGAANAMAQGRVYIGGNIGARGMTMTKYNPRFEPPELWVFGKAGDSFAEFMA
                     GGIAVICGAGAEEEKNVLGYRPCVGMVGGKIFFRGSQEAYSQPDARLVTDLPDDEWEW
                     LKQNLKTFLDAIGRQYLFEQLAEKREDWQLLIARKPSEKASRFVRPMAKFKQEVWEHE
                     LGRGGLIGDLSSLDRSPIDVITTGALRRFVPSWENEKYLPPCQAACPTGIPVQKRWEL
                     IRKGHLEEAVNLALEYTPFPAAVCGHLCPNLCMQSCTRNLVKLDPVDVRVLGKASLQA
                     KVPRRAPETGKKIAVVGGGPAGLSVAWQLWLSGHDVTIVESRKQLGGKITDSIPKSRI
                     PAEVVEHEIARLARNLTRTQTGQALTRQGFAKLKNDHDFVVIAAGAQSPRKLPVPGMD
                     RTISALEFLRQSKLDCANAGEKVVIIGAGNVGCDAATEAFRLGAKSVTLIDVQEPASF
                     GVERQHAEAAGAVFLWPRVTKAVTDSGVELTSGDVLPADTVIVAVGELPDLSFLPEEI
                     ATERGFVLVDDTYATSDSQVYAIGDAVRPGLLTDAIGAGRIAARSIDDSIRGAHESYD
                     KLPPIRRDRVKLQYFDPRAAGMSDPASCATRCASCGACRDCGLCEEICPEQAISRIQT
                     AGGKFRYEVNAERCIGCGFCAGACPTGVWEIAENKPLA"
     gene            8094..10271
                     /locus_tag="PLA58_06135"
     CDS             8094..10271
                     /locus_tag="PLA58_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOR62410.1"
                     /translation="MKKTDTIAAAERSYIIEKEKFVPVSQYFGEDTFNHKVMKDKLPK
                     NIYKKLMDAINEDMPLDADTANVVAHAMKEWALEKGATHFAHWFQPMTGITAEKHDAF
                     ADPIAPGEVIERFSGKQLVQGEPDASSFPSGGIRATFEARGYTAWDMSSPAFIKRNGI
                     STTLCIPTAFISYTGQALDKKTPLLRSIRALSKSAVAMLKLLGVKNVKKVMSNLGPEQ
                     EYFLIDMDYFYKRQDLVLGGRTVVGAAPAKGQELEDQYFGSIKERISSFMHDVEEELF
                     KLGIPAKTRHNEVAPSQYEIAPVYEEANLAIDHNQLVMETLRSVAKKHKLAALLHEKP
                     FAKINGSGKHVNWSMADNNDNNLLNPGTTPQDNIQFLVFLIATVRAVYKHADILRASV
                     ASYGNDHRLGANEAPPAIISVFLGEQLTQILDNIEKGTVTKATNAEIIDLGVSSLYQV
                     SKDNTDRNRTSPFAFTGNKFEFRAVGSSQSIATQVIALNTIVAESIDELAEKIKAKTR
                     NAKNINQAVFDVLRSEIKSVRPILFNGDNYTREWEAEAKRRGLPNEKTTPTALRALVT
                     DKALDLFEKYEVLSRVELKSRQLIHVERYIKDLEIEAKCLNNICMTQIIPAAVSYQEK
                     LAKAIHAAKEAIGSGAVVSSQTELLKKILDLIHTIYVTNKDILARVEAAEAVHDEQKK
                     AETLAARVKPKMDELRESVDALENLVDDERWPLPKFWEMLFIC"
     gene            10344..11948
                     /locus_tag="PLA58_06140"
     CDS             10344..11948
                     /locus_tag="PLA58_06140"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOR62411.1"
                     /translation="MKNTKFIFVTGGVLSSLGKGLAAASIAAVLESRGLRVSNQKLDP
                     YINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFSSTRMGKRNNLTTGQVYFSVIT
                     RERRGDYLGKTVQVIPHITNEIKDFIRETAKGFDVSIVEIGGTVGDIESLPFLEAIRQ
                     FRNEVGRQNAIFIHLTWVPFIKTAGEVKTKPTQHSVKALREIGIQPDILLCRTENFLS
                     DEIKGKIALFCNVEVNCVFTAKDVGCIYECPLVYHREGLDAKIIELLNMWTGQPKLTA
                     WENVVEKFYHPAHEVCIGIAGKYVNLTDSYKSLNEALVHGGIANNCRVKLKFVDTEKI
                     EQDGAASHLSDVDGVLVPGGFGNRGIEGMILSARYARENQIPYFGICLGMQMAVIEYA
                     RDICGLASANSSEFDPACPHPVIDLLPQQRTVRDKGASMRLGAWPCAIAESSFAFEAY
                     GQKEISERHRHRYEFNNDFKKMLTEKGLRITGASPDGTLAEIIEISDHPWFLGCQFHP
                     EFKSRPTSPHPLFSKFIEAALKQSGT"
     gene            11989..13119
                     /gene="carA"
                     /locus_tag="PLA58_06145"
     CDS             11989..13119
                     /gene="carA"
                     /locus_tag="PLA58_06145"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOR62412.1"
                     /translation="MSLFKKNRRALLVLEDGSVFEGSAFAGAGEALGEVVFNTGMTGY
                     QEIVTDPSYKGQIVAMTYPLIGNTGVNDEDMESSDLHLEGFIVREYSSRPSNWRMTKS
                     LKDFLEDAGKIGLEGVDTRSLTRRLRLSGSMKGILSTESGDVPALLEKARHYPGLVGR
                     DLVRDVSCAEPYCWEKGAPGTVSLPPGKSAGKPRVVVLDCGVKYNILRILESNGCEVI
                     VLPATATADQMLSYHPDGILLSNGPGDPSALPYLVDAARGVIGKTPVFGICLGHQILG
                     QAVGGKTQKLKFGHHGVNQPVKNLETGRVEISSQNHGFVVVPESVGGKASPTFTNLND
                     ATSEGMAIAGAMSVQYHPEAAPGPHDTEYLFSRFVAMMKKQR"
     gene            13123..>15725
                     /gene="carB"
                     /locus_tag="PLA58_06150"
     CDS             13123..>15725
                     /gene="carB"
                     /locus_tag="PLA58_06150"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOR62413.1"
                     /translation="MPKRTDIQKILLIGSGPIIISQACEFDYSGTQACKALREEGYEV
                     VLINSNPATIMTDPEMADRTYVEPITPEAVEKIIARERPDALLPTLGGQTGLNTAVAL
                     AESGVLARYGVEMIGASLPVIHKAEDREKFRQAMERIGLTMPKSGFARNMEDVRRIAQ
                     DIGFPIIIRAAFTLGGTGSGVAYNWEELEEIAKAGLDASMISEVMVEQSALGWKEYEL
                     EVMRDRADNVVVICSIENFDAMGVHTGESITVAPAQTLTDREYQKMRDAAIAIMREIG
                     VETGGSNVQFAVHPQTGEMIVVEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITRETMASFEPAIDYVVTKIPRWTFEKFPETEDFLTTSMKSVGETMAIGRTFKE
                     SLQKAIRSLETGRFGFGQALPDEEQARGELLKSKLIKPNSLRLFYIAEALKSGLTIDE
                     VYDLTKVDPWFLHQMKDILNAEEELKNATPSAELLRAAKKLGFSDRALGALWNKTEFE
                     IRDWRKRENITPVYKLVDTCAAEFEAYTPYYYSTYEQESETRPSAKKKVAIIGGGPNR
                     IGQGIEFDYCCVHASFALSEEGIESIMINSNPETVSTDYDTSDKLFFEPVTLEDVLHI
                     LEAEKPDGVIVQFGGQTPLNLARGLEEAGVPIIGTSPAAIDRAENRDRFQEIVSKLNL
                     NQPDNDTAMDVEEALAAASRIGYPVLVRPSYVLGGRSMEIVYDPETLRTFMARALLVS
                     PGHPILIDKFLEDAVEVDVDAISDGRTTVVAGIMEHIEEAGIHSGDSACILPPVTIAP
                     PMVKLIEAQTKMLAAEIGVVGLMNIQYAIKDGKLYVLEVNPRASRTVPFVSKAIGVPL
                     AKLATRVMLGKT"
BASE COUNT         3695 a         4475 c         4521 g         3034 t
ORIGIN      
        1 aaagactgca agaatcagga tcagcaactg actgcggacg gacttcatca ttgaaaaatc
       61 aacgcctcct atctctgaga agatgaatct caacgagtga tttgtcatat atcataagtt
      121 ctgaagaaac atcaacgatt acgaatggaa acaaattcag agcattcgtg ataccgtttg
      181 ttctcatatc ttattctcac atcggatcgg ctcgctttaa agcagggtaa ccgctttaaa
      241 aacggttcgg ggatacaatt atgtctgttt gtgttcaaaa cggacaaaca tgtatccttt
      301 gaaaatcatc tggatcacat aaccatttaa atataaaaac gaatttcaaa ttctaccact
      361 ggcacggcgg ttgctcccga ctcatttgtg ttatcgattg ggaaaggaag cgctttctga
      421 ctcaagatgg ttttgttctt ctctgagggg tcgtctaaag gtaggacgcc aggttctggc
      481 cctgaaaatt gaggttcgag tccttgcccc tcagccatct gctccatttc tgaaaggcaa
      541 atgctgatga aactgatcat cgccatcgta caatccgaca agatcaaaga cgtcaaacag
      601 gctttgcgcg acgtaaacgt gggcaaaata accgtctcca gtgtagtcgg ctgcggcgcg
      661 caggtcggca aaaccgatac ccgcgacgga gtagtcacca acgggaacct cctcgaccgg
      721 gtgcgtttcg aaatcgccgt gaacgacgac tacgtcaaac ccacgctgga agccgtcatc
      781 cggggcgcgc gcaccggcag tatcggcgac ggtaaaattt tcatcgtccc tctggaggaa
      841 tgcatccgga tccgcacggg cgaaaccggc cggctggcca ttggatgaag cttcccggaa
      901 ccttcccaaa gcgacatttc ctgctctttt tttatccaac acgcaaaatg gttttctctt
      961 tgcatgagca tcctgggacg gacaaaatac cggttgatat tcgtacaaat ttgtttcatt
     1021 gtaccggggc gggccaggaa gcgaccggta aaaacccgaa gccgaatcag gggagcgaga
     1081 caacgtgagc atccacagtt atgaagaatt agcggccctt cacgccatat ccaagatttt
     1141 ggcgcagccg atggaattga gagaccaact ggagcaaatc cttcatgaaa tgagtttgcg
     1201 cctcgggatg cagcgcggca tgatatcgct tctggatcac gagaaaaagg aagcgtggct
     1261 ggacattgcc catgacgtca acattgacgg gatggaagtg acttacaaac cgggagaagg
     1321 cattaccggc aaagtggcgg aaaccggccg cccgatggcc gtggcaaatc tggggcagga
     1381 agccctcttt ctggaccgca ccggggcaag gcgtcatctg aaccgcgccg agctttcttt
     1441 tttatgtgtt cccattatct atgacgcaag cgtggtcggc gttttatccg ccgacaaggt
     1501 ggccaagcaa gtcgaagacc tggaaaagga actggccatg atgggctcgg tggccgaact
     1561 gatggccaaa gccgtgcata tccgggccct ggaagaagaa aaccgccgcc tgcgcaaaat
     1621 tgtcggacag cagcatgcgc cttccatgga tattatcggc cattccaaag ccatccagga
     1681 agtcttcggg ctcgtggcgc aggtttccga ttccaatacg acggtcctga tcaccggcga
     1741 aacgggaacc ggcaaggagt tgattgcccg ggcgattcat aacaactctc cgcgcaaagg
     1801 gggaccgctg gtgcaggtca actgcgcggc gattcccgac acgctcattg aaagtgaact
     1861 gttcggccat gaaaaaggct catttaccgg cgcgctgcag acgcgccgcg gccgcttcga
     1921 agaagccaac ggcggcacga tctttttgga tgaagtgggc gaactgtccg ccgccgcgca
     1981 ggtaaagctt ctgcgcgtgc tgcaggaaaa gcgatttcag ccgctgggct cgtcgcgggt
     2041 ggtgaatgtc aatgtacgaa tcatcgccgc gacgaaccgt aatctggagc aggacattct
     2101 cgccgaccgt ttccgtgcgg atctttttta ccgccttaat gttttcccca tttatctgcc
     2161 gccgctcagg gaacgcggca gcgatgtgat tttgctggcc gatcattttc tgctcaagta
     2221 cagcagggaa atgggcaaga acgtcaagcg gatctccacg gcggccatcg aagcgtttct
     2281 gtcccataaa tggccgggca acgtccgcga actggaaaac tgcatcgagc gggcggtttt
     2341 attgaccagg acggacaccg tggactgcct gcacctgccg ccttctctgc atgccggaga
     2401 aaaagacccg gaacgaaaag accgcagcaa gctgtcgtcc gttgtggagg cgcaggagag
     2461 gacgctgatt atcgacgcgc tcacggaaag cggcggcaat cagaccaaag cggccaaaat
     2521 cctgggaacc acgaagcgca ttattcagta taaaatatcg aaaatgggca tcgacccgaa
     2581 aaaattcagc cggaaaaaaa aaggatctga ggcctagacg aaaaaggtat ttggtcaacc
     2641 gtccgcagag ctatcgtatt gcagaagcgc cggacctgcc gcccgggcgc tcccaaatca
     2701 tgcgggaatc ataaacctcg tttcatgaaa ccttctcgcc cccagcaaat ccgaacaaaa
     2761 ttgtcttgat gttttacaaa agaacatttt tgttgcctga atcaaagcac aggatcgtct
     2821 ttgcgctctt ccaaaaagct aaccgcttat aattcataga taaactatat tgatcccgca
     2881 ctggcacgac tctggcaatc agcttgtcgg gttgaaatgc acagaggaga ggctatgtgt
     2941 cgtttatttg ctgtaacatc caataatccg ctgtcgccga tgatggccat caacgcgctg
     3001 aatgtcatga aagagggaca tgacggatcc ggcgtggggt tgttcatgac cgatctgggg
     3061 ggcgagttcg aaaaattcaa aagcgagccg atcctgtccg gcatcttttc caatgaaggc
     3121 atcaaagcgc tcgaccgctt catgatcgat caggatttca tggtcaaata caagctgtcc
     3181 ttccggccgg caaaaccgac gcctccggga acaccgaaaa gggataacta cgtcatccgc
     3241 gtttacgaat acccggccga atgggaaagc ttaagccggg aggaaatcga ttaccgcctg
     3301 atgatggtgc agctccagct tcggcagatg ggcgaaaaag atcaatccat gatggtcttc
     3361 agcttctggc cggatgtgat catcatcaag gaagtgggcg atccgctggc cgtggccgag
     3421 tatctggggc tggaccgcaa ggacctcacc gcccggatga tcttgagcca gggacgccag
     3481 aacaccaact actccattga tatttacgcc tgtcacccgt ttttcatcca gggcatggcg
     3541 acgatgacca acggcgagaa caccgcgttt gtgccgatcc gggaatttct gatgtcgcgc
     3601 aatttccccg gctacaccgg ctaccagtcc gactcggaag tgttcacaca catcctgcat
     3661 tacacgcaga acaaactcgg actgggcatg gaaatgtaca agcacgtgat cactccgctc
     3721 agggacgagg agctggcgcg tcatcccgac gggcggatgc tcagaaacct gaagcagagc
     3781 tgccggcctc tgatcatcga cgggcccaac tgcgtcatcg gctgtctgcc ggacaagtcg
     3841 atgttcatgg tgcaggacgc caagaaactt cgccccggcg tggtgggcgg ccgtcccggc
     3901 attttcgcgt tttcctcgga aatgtgcggc ctggatgccg ccattccgga gcgcgacatc
     3961 aacctggacg atcagccgat gagatatgaa accgtcattg tccgccgcgg gcggcaggag
     4021 atagaaaaat ggaaccagtg ggacacattg ccccatctac actaagcgcc aaagacctgc
     4081 cctggcagat tgcctggcaa aaggatcggt gtaccctgtg cggccgttgc acaaccgtct
     4141 gcccggtgcg ggccatcgaa ctgggcgtcc accgcaaacg cgcgctcacg acgccggtcg
     4201 gtctggagag ccgtcccgcc aatctgtttt ccgtgtacca cggcatcgat cagcgcaccg
     4261 atccggccca tgcctgcgtg ggctgcggca tgtgtacgct ggtctgcccc aacaacgcca
     4321 tcgtccccct gcgcgccgat gaaaccgaca aactgcgctt tcacgtcaac cagggcggcg
     4381 aagcgaggcg acggggcggc cggagaaacg ctccggaaag cgtgctcgac cagatcaagt
     4441 ttgtccgcat ttccatgctg accgacccgg cgctcgacgc cggacgacat gaatttgagc
     4501 tgcggaccct gctgggaaga atcctgccgc cggagcggat gctccaggcc ggccgtgaaa
     4561 acggctggat gccgcccgtt cgcgagattt atccgatgat catcggcggc atgtcgtttg
     4621 gcgcgctctc cccgaacatg tgggaaggtc tgcagatggg cgtggcgtat ctgaacgagg
     4681 agatgggcat gccggtccgt atctgcaccg gcgagggcgg ctgcccgccc cggctgctca
     4741 agtcgcgctt tctgaaatac gtcatcctgc agatcgccag cggctatttc ggctgggatg
     4801 aaatcatcca caacctgccg cagatgaagg aagacccctg cgccattgaa atcaagtacg
     4861 gacagggggc gaagcccggt gacggcggtc ttttgatgtg gtacaaggtc aaccggctga
     4921 ttgccgcgat tcgcggcgtc ccgcagggca tcagccttcc cagcccgccg acgcaccaga
     4981 ccaagtattc gattgaggaa gccgtggcca agatgatcca gtccatgtcc atggcgtggg
     5041 gcttccgcgt gccggtgtat ccgaaaatct cggccaccag caccacgctg gccgtgctca
     5101 acaatctgac ccgcaatccg tacgcggccg gactcgccat cgacggcgag gacggaggca
     5161 cgggcgcggc ctacaatgtt tccatgaacc acatgggaca ccccatcgcc agcaacctgc
     5221 gcgacgccta cctgacgctc gtgaagctgg gcaaacaaaa cgagctccct ctctttgccg
     5281 gcggcggcat cgggaaaaac ggcaatctgg ccgccaatgc cgccgccctg atcatgctgg
     5341 gcgccagcgg tgttcagacg ggcaaataca tcatgcaggc ggccgccggc tgtctgggat
     5401 cggagggcga ccgctgcaat gtctgcaacg tcggcgtttg ccccaaaggc atcacctcgc
     5461 aggacccgcg gctggatcgg cggctggacc cggagcgggt tgcccagcgg ctggtggacg
     5521 tctatttaac gttcgacacg gaactgaaaa agatcgtggc gccgctgggg cgctccactt
     5581 cactgccgat cggcatgtcg gatgccctgg gcatcagcga tgcggacgcc gccgggcggt
     5641 tgaacatcaa atacgttctg tagtggggaa cggttttaaa ccgttcccta cggtaacgcc
     5701 acggaattgg aaaacagggt aagacgatga ccagaaaaga tacgatcaaa attgccggcg
     5761 tggaaaacac cgtccgcgtg gattcacgga ttctggaaga acgcattcag aaagccgtgg
     5821 cagacggttg ccgtcatatt gaagtcaccg ccctggggca gcacggcatt ggcggccgtc
     5881 tctggcgggc gcacgacgaa ccggtgaagg tcgatgtgct gggcacgtcc ggtcagcgcc
     5941 tggggtccat gggatttgcg aacaccacga ttcaagcatt cggccccgcc tctgacgacg
     6001 tcggctggct caacgcgggg gcaaaaatca ttgtgcacgg aaatgccacc aacggcgcgg
     6061 caaacgccat ggcgcaggga cgggtgtaca tcggcggcaa catcggcgcg cgcggcatga
     6121 ccatgaccaa atacaacccg cgctttgagc cgcccgaact atgggttttc ggcaaagcgg
     6181 gagactcgtt tgcggaattc atggcgggcg gcatcgccgt gatttgcggc gcgggcgcgg
     6241 aagaagaaaa aaacgtgctc ggataccggc cctgcgtcgg catggtcggc ggaaaaatat
     6301 tcttccgcgg cagtcaggaa gcttacagcc aacccgatgc ccggctggtg accgaccttc
     6361 cggacgacga atgggagtgg ttgaagcaaa acctcaaaac atttctcgat gccatcggca
     6421 ggcagtattt atttgaacag ctcgcggaaa aacgggaaga ctggcagctt ttgatcgccc
     6481 gcaaaccttc tgaaaaggcc tctcgattcg ttcgtccgat ggcgaaattc aaacaggagg
     6541 tctgggaaca tgagctgggc cggggcggtt tgatcggcga tttgtcgagt ctggaccgtt
     6601 ctccgatcga cgtgattacc accggagcgc tccggcgctt tgttccctcc tgggaaaacg
     6661 aaaaatatct tccgccctgc caggcggcct gcccgaccgg cattcccgtt caaaaacgat
     6721 gggaactgat ccgcaaaggc catctggaag aagccgtgaa tctggcgctg gaatacaccc
     6781 ccttccccgc ggccgtctgc ggccatttgt gtccgaacct ctgtatgcag agctgcacgc
     6841 ggaacctggt gaaactcgat ccggtggatg tgcgggtgct gggaaaggcg tcgcttcagg
     6901 caaaagtccc ccggcgcgcg cccgaaacag gcaaaaagat cgccgtcgtg ggcggcggtc
     6961 ccgcgggcct gtctgtggcc tggcaattgt ggctctccgg ccacgatgtc acgattgtgg
     7021 agagccgcaa acaactgggc ggcaagatca ccgactcgat acccaaaagc cgcattcccg
     7081 cggaggtggt cgagcatgaa atcgcgaggc tggccagaaa tcttacccgg acacaaaccg
     7141 gccaggcgct gacccggcag ggttttgcca aactcaaaaa cgatcatgac tttgtggtca
     7201 ttgcggcggg tgcgcaaagc ccgcgcaagc tccctgtgcc ggggatggac agaaccataa
     7261 gcgcactcga gtttttgcgg caaagcaagc tcgattgcgc aaacgcaggc gagaaggttg
     7321 tcattatcgg cgccggcaat gtcggctgtg acgcagcgac ggaggctttt cgcctgggcg
     7381 cgaaaagcgt gacattgatt gacgttcaag aacccgcgtc gttcggcgtg gagaggcagc
     7441 atgccgaagc ggcaggcgcg gtgtttctgt ggccgcgtgt cacaaaagcg gtgacggatt
     7501 cgggcgtcga gctgacgtca ggcgatgtgc tgcccgccga tacggtcatc gtggcggtag
     7561 gcgaactgcc ggacttatcc ttcctgccgg aagaaattgc gaccgagcga ggttttgtgc
     7621 tggtggacga cacgtacgcc accagcgact ctcaggttta cgccatcggc gatgcggtgc
     7681 ggccgggtct tttaaccgac gccatcggcg cgggcagaat cgccgcgcgg tccatcgacg
     7741 attcgatccg gggagcacac gaatcctatg acaaattgcc gccgattcgc cgtgatcgcg
     7801 tcaagctgca atacttcgac ccccgcgccg cgggaatgag cgatcccgcc tcctgcgcga
     7861 ccagatgcgc ctcctgcggc gcgtgccggg actgcggcct gtgcgaggaa atatgccccg
     7921 aacaggccat ctcccgcatc cagaccgccg ggggcaagtt tagatacgaa gtcaacgccg
     7981 agcgctgcat cggttgcggt ttctgcgccg gggcctgtcc caccggcgtc tgggaaatcg
     8041 cggagaataa acctttagcg taaaccattt tttcagataa aggatgaaaa accatgaaaa
     8101 aaacagatac catcgcggcc gcagagagaa gctatatcat cgaaaaggaa aagtttgtcc
     8161 ccgtctcgca gtatttcggc gaggacacct tcaaccacaa ggtcatgaag gataagcttc
     8221 ccaagaacat ctacaaaaag ctgatggacg caatcaacga agacatgccc ctggatgcgg
     8281 atacggccaa tgtcgtcgcg cacgccatga aggaatgggc gctggaaaag ggcgcgacgc
     8341 attttgcaca ctggttccag ccgatgacgg gcatcacggc ggaaaagcac gacgcctttg
     8401 ccgatccgat cgcaccgggc gaagtgattg agcgattctc cggaaaacag ctcgtccagg
     8461 gcgagccgga tgcttcgtct ttcccctccg gcggcatccg cgcgactttt gaagcgcgag
     8521 gttacacggc ctgggacatg tcctcgcccg cgtttatcaa gagaaacggc atttcaacaa
     8581 ccctgtgcat tccgaccgcc ttcatttcct acacgggtca ggcgctcgac aaaaagacgc
     8641 ctcttttgcg atccatccgc gcgttgagta aaagcgccgt ggcgatgctg aagctcctgg
     8701 gcgtcaaaaa tgttaaaaag gtgatgtcca atctcggccc cgagcaggaa tatttcctga
     8761 tcgatatgga ctatttttac aaacgccagg acctggtcct gggcggccgg acggtggtcg
     8821 gcgcggctcc ggccaagggg caggagctgg aagatcaata cttcggctcg atcaaggaac
     8881 gaatttcctc ctttatgcat gacgtcgagg aagaactctt taaactgggc attccggcaa
     8941 aaacccgcca caacgaagtg gcgcccagtc agtatgagat cgcccccgtt tatgaagaag
     9001 ccaatctcgc cattgaccac aaccagctgg tgatggagac gctgcgcagc gtggccaaaa
     9061 aacacaagct ggccgcgctc ctgcacgaaa aaccgtttgc caaaattaac ggttccggca
     9121 agcacgttaa ctggtcgatg gccgacaaca acgacaataa cctgctcaac cccggcacga
     9181 caccgcagga caacattcag tttctggtgt ttctcatcgc gacggtccgc gccgtttaca
     9241 agcatgcgga cattctgcga gcctccgtgg cctcctacgg gaacgaccac cgcctgggcg
     9301 ccaatgaggc gccgccggcg atcatctccg tgttcctggg cgaacagctc acgcagattc
     9361 tggacaatat tgaaaaaggc accgtgacga aagccacgaa tgccgaaatc atcgatctgg
     9421 gcgtctcgtc tctgtatcag gtcagcaagg acaataccga ccgcaaccgg acgtcgcctt
     9481 tcgcctttac cggaaacaag tttgaattcc gcgcggtagg ctcctcgcag tccatcgcca
     9541 cgcaggtcat cgctctcaac acgatcgtgg ccgaaagcat cgatgagctg gccgagaaga
     9601 tcaaagccaa aaccaggaac gcaaagaaca tcaatcaggc cgtgtttgac gtgctcagaa
     9661 gcgaaatcaa atccgtccgt ccgatcctgt tcaacggtga caactacacc agggaatggg
     9721 aagccgaagc caaacggcgc ggacttccca acgaaaaaac cacgcccacg gcgcttcgcg
     9781 cgctcgtcac cgacaaggcg ctggatttgt ttgaaaaata tgaagtcctc tccagggtgg
     9841 agctcaagag ccgccagctg attcatgtag agcgttacat taaggatttg gaaattgagg
     9901 cgaaatgtct caacaacatc tgcatgacgc agattattcc ggccgccgtg tcgtatcagg
     9961 agaaactggc caaagcgatc catgccgcga aggaagcaat cggttccggc gcggtggttt
    10021 catcgcagac ggagctcctt aaaaagatcc tggatctgat ccatacaatt tacgtcacca
    10081 acaaggacat cctggccagg gtggaagcgg ccgaagccgt tcacgatgag cagaaaaagg
    10141 cggaaaccct tgccgccagg gtcaagccga agatggatga gctgcgggaa tccgtggatg
    10201 ccctggaaaa tctggtggat gatgagcggt ggcctctgcc caagttctgg gaaatgttgt
    10261 ttatctgctg atacagacgc aggggcggcg ttccccgccg tcccggctct cttgaaggcc
    10321 atggaattgc aagaggacca actgtgaaaa atacaaaatt tatttttgtg accggcggcg
    10381 ttctgtcgtc gctgggcaag ggcctggcgg cggcatcgat tgcggccgtt ctggaatcac
    10441 gcgggcttcg ggtgtcgaat caaaagctgg atccgtatat taatgttgat ccggggacga
    10501 tgagcccttt tcagcacggg gaggtcttcg tcaccgacga cggcgcggaa acggatcttg
    10561 atcttggcca ctacgaaaga ttttcctcca cacggatggg aaagcgcaac aacctgacaa
    10621 ccggacaggt ttacttttcc gtcattacca gggaaagacg cggcgactac ctgggcaaaa
    10681 ccgttcaggt catcccgcat atcacgaacg aaatcaagga tttcatccgc gaaaccgcga
    10741 aagggttcga cgtttccatc gtggaaatcg gcggaaccgt aggcgacatc gaaagccttc
    10801 ccttcctgga agccatccgc cagttccgca acgaagtggg ccggcaaaac gccattttca
    10861 tccatctgac ctgggtgccg tttataaaaa ccgccgggga agtgaagacc aaacccacgc
    10921 agcacagtgt gaaggctttg cgggaaatcg gcattcagcc ggatattctg ttgtgccgga
    10981 cggaaaactt tctgtccgac gagattaaag gcaagattgc tctgttttgc aatgttgaag
    11041 tcaactgcgt ctttacggcc aaggatgtcg gctgcattta cgagtgtccg ctggtgtatc
    11101 accgcgaagg actcgatgca aagattattg aactgctcaa catgtggacc ggacaaccga
    11161 agcttaccgc ctgggagaat gtggtcgaaa aattttatca tccggcccat gaagtctgca
    11221 tcggcatcgc gggcaaatac gtcaacctca cggattccta caaaagcctg aatgaggcgc
    11281 tcgtgcacgg cggcatcgcc aacaactgcc gggtaaaact gaaatttgtc gatacggaaa
    11341 aaatcgagca ggacggcgcg gcaagtcatc tgtcggacgt ggacggcgtg ctggtgccgg
    11401 gcggcttcgg caaccgcggc atcgaaggca tgatcctgtc ggcgcgctat gcgcgggaaa
    11461 accaaatccc ctatttcggc atctgtctgg gcatgcagat ggcggtgatc gaatacgccc
    11521 gcgatatctg cggcctggcc agcgccaaca gctccgagtt tgatccggcg tgtccgcatc
    11581 cggtcattga cctgctgccc cagcagcgca ccgtgcggga caagggcgcg tccatgaggc
    11641 tgggcgcatg gccctgcgcc attgccgaat cctcctttgc ttttgaagct tacggtcaaa
    11701 aggaaatctc ggagaggcac cgccaccgct atgaattcaa caatgacttt aaaaagatgc
    11761 tgactgaaaa gggtctgcgc atcaccggcg cgtcgccgga cggcacgctg gcggagatca
    11821 ttgaaatttc cgaccacccg tggtttctcg gatgccagtt tcacccggaa ttcaaatccc
    11881 gtccaacctc gccgcatccg ctgttcagca aatttattga ggcggcgttg aaacagtccg
    11941 gaacgtgatc cggcaggcgc cgaaggtcgc caggagataa acagccccat gtccctcttt
    12001 aaaaaaaatc ggcgggcgtt gctcgttctt gaagacggaa gcgttttcga aggttctgcc
    12061 tttgccggcg caggagaggc cctgggtgaa gtcgtcttca acaccgggat gaccggctat
    12121 caggaaattg tcaccgaccc ttcctacaaa ggccagatcg tcgccatgac gtatccgctg
    12181 atcggcaaca ccggcgtcaa tgacgaagac atggagtcgt cggaccttca tctggagggc
    12241 ttcatcgtca gggaatattc atcgcgcccc agcaactggc ggatgacgaa aagcctcaag
    12301 gattttttgg aggatgccgg aaaaatcggt ttggagggcg tggacacgcg ctccctgacc
    12361 cgccgcctgc ggctgtccgg ttcgatgaag ggcatccttt cgaccgagtc cggagacgtc
    12421 cccgctctgc tggaaaaagc gcgccattac ccggggctgg tcggccggga tctggtgcgg
    12481 gacgtgtcct gtgccgaacc ctattgctgg gaaaagggcg cgcccggaac cgtcagcctt
    12541 ccccctggga aatcagcggg aaagccgcgg gtggtcgtgc tggactgcgg cgtcaaatat
    12601 aatattctaa ggattctgga atcgaacggc tgcgaagtga ttgttctccc cgccacggcg
    12661 acggccgatc agatgctgtc ctatcatccg gacggcattc ttttatccaa cggaccgggc
    12721 gatccgtccg ccctgcctta tcttgtagat gccgcccgcg gcgtgatcgg gaaaacgcct
    12781 gtcttcggaa tctgcctggg gcatcagatt ctcggccagg ccgtgggcgg gaaaacccaa
    12841 aaactcaagt tcggccacca cggcgtcaat cagcctgtga aaaatctgga aaccggccgg
    12901 gtggaaattt cgtcccaaaa ccacggtttt gtggtcgtcc cggaatcggt cgggggaaaa
    12961 gcgtccccga cctttacaaa cctcaacgac gcaacctccg aagggatggc gattgccggc
    13021 gcgatgagcg tgcaatatca ccccgaggcg gcgccggggc ctcacgatac ggaatatctg
    13081 ttttcccggt tcgtggccat gatgaaaaag caaaggtaac ccgtgccgaa acgaaccgac
    13141 attcaaaaaa tactgctcat cgggtccgga ccgattatca tcagccaggc ctgtgagttt
    13201 gactactccg gcacacaggc ctgcaaagcc ctgcgcgaag aagggtatga agttgtcctc
    13261 atcaactcca acccggccac catcatgacc gatccggaaa tggccgaccg cacctacgtg
    13321 gagccgatca cccccgaggc cgtggaaaaa atcatcgcgc gcgagcggcc ggatgcgctt
    13381 ttgcccacac tgggcggaca gaccggcctc aataccgccg tggcgctggc cgaatcgggc
    13441 gtcctggcca ggtacggcgt ggaaatgatc ggcgcgtcct tgccggtcat tcacaaggcc
    13501 gaagaccgcg aaaagttccg ccaggcgatg gagcgcatcg gcctgaccat gcccaaaagc
    13561 ggctttgccc gcaatatgga ggacgtccgg cggatcgcac aagacatcgg tttcccgata
    13621 atcatccgcg ccgccttcac cctgggcggc accggcagcg gcgtggccta caactgggaa
    13681 gaactcgagg aaatcgccaa ggcaggcctc gacgccagca tgatctccga agtgatggtc
    13741 gaacagtcgg cgctgggttg gaaagaatat gagctggaag tgatgcgcga ccgcgccgac
    13801 aacgtcgtcg tcatctgttc gatcgaaaac ttcgacgcga tgggcgtgca caccggcgag
    13861 tcgatcaccg tcgcccccgc gcagacgctc accgaccgcg aatatcaaaa aatgcgggat
    13921 gccgccattg ccatcatgcg ggaaatcggc gtggaaacgg ggggctccaa tgtgcagttt
    13981 gccgttcatc cgcagaccgg agaaatgatt gtcgtcgaga tgaatccccg cgtgtcgcgc
    14041 tcgtcggcgc tggcctccaa agccacggga ttcccgattg ccaagatcgc cgcgaagctt
    14101 gcggtgggct acacgcttga tgaaattccc aacgatatca cccgcgaaac catggcatcc
    14161 tttgaacccg cgattgacta tgtggtgaca aaaattcccc gctggacgtt tgagaaattt
    14221 cccgagacgg aagatttcct cacaacgtcg atgaagtcgg tgggcgaaac catggcaatc
    14281 ggccggacgt tcaaggaatc gctgcaaaag gccatccgct cgctggagac cggccgcttc
    14341 ggctttggcc aggcgctgcc tgatgaggag caggccagag gcgaactttt gaaaagcaag
    14401 ctcatcaaac ccaattccct gcggcttttt tacatcgccg aagcgctgaa aagcggcctg
    14461 acgattgacg aggtctatga tttgaccaaa gtcgatccct ggtttttgca ccagatgaaa
    14521 gacatcctga acgccgaaga agagctgaaa aacgcgacac cgtccgccga acttttgcgc
    14581 gccgcgaaaa aactcggttt ttccgaccgc gcgctggggg cgctctggaa taaaacggag
    14641 ttcgaaatcc gcgattggcg caaacgggaa aacattacgc ccgtctataa actggtggac
    14701 acctgcgcgg ccgagtttga agcctacacg ccttattact attccaccta cgagcaggaa
    14761 agcgaaaccc ggccctcggc gaaaaagaaa gtcgccatta tcggcggcgg ccccaaccgc
    14821 atcggccagg gcattgaatt cgactactgc tgcgttcatg cctcgtttgc gctctccgag
    14881 gaaggcatcg aaagcatcat gatcaactcc aacccggaga cggtcagcac cgactacgac
    14941 acttccgaca agctgttttt cgaaccggtc acgctcgaag acgtcctgca cattctcgag
    15001 gcggaaaaac ccgacggcgt gatcgtgcag ttcggcggcc agacgccgct caatctggcg
    15061 cgcggcctgg aagaagcggg tgttccgatc atcggcacat cgcctgccgc catcgatcgg
    15121 gcggaaaacc gcgaccgctt ccaggaaatc gtcagcaaac tgaacctcaa ccagccggac
    15181 aacgatacgg ccatggacgt ggaggaggcg ctggccgccg cctcccgcat cggatacccg
    15241 gtgctggtgc gtccctccta tgtgctgggc ggccggtcca tggaaatcgt gtatgatccc
    15301 gaaaccctca ggacgttcat ggccagggcg ctcctggttt cgccggggca tccgattctg
    15361 atcgacaagt tcctggaaga cgcggtggaa gtcgatgtgg acgcgatttc cgacggccgg
    15421 accaccgtcg tcgccggcat catggagcat atcgaggaag cgggcattca ctcgggtgac
    15481 tccgcctgca ttttgcctcc cgtcacgatt gctcctccga tggtgaaact catcgaagcg
    15541 caaacgaaaa tgctggccgc agaaatcggc gtggtgggct tgatgaatat tcagtacgct
    15601 attaaggacg gcaagctgta tgttctggaa gtcaacccgc gcgcgtcgcg aacggtgcct
    15661 tttgtgagca aggccatcgg cgtgccgctg gcgaagcttg ccacccgggt catgctgggc
    15721 aaaac
//
LOCUS       DAOSRM010000049        15685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3865_length_15685_cov_97.619194, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000049 DAOSRM010000000
VERSION     DAOSRM010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15685
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3865_length_15685_cov_97.619194"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLA58_06155"
     CDS             <1..654
                     /locus_tag="PLA58_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOR62414.1"
                     /translation="EGALQIIDGKAKVVSETFCDGLGACIGECPTGALKIVERKASPF
                     DEEAVRRHLSKKEPETMPCGCPAAHIQIFDKPIACEAANKPKHFESAEEESALSHWPI
                     QIKLIPAGAPFLKGADLLVLADCTAVAFPSLHRNLLKGRVVMMGCPKFDDVQEYVQKF
                     ADIFRTADIKSVTVAVMEVPCCSGMPMIVRKGMEAANRKIPLTEIVLSLRGKILKKN"
     gene            complement(718..1893)
                     /locus_tag="PLA58_06160"
     CDS             complement(718..1893)
                     /locus_tag="PLA58_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HOR62415.1"
                     /translation="MLFKIKTVVFAVVVSVFLAFSVAGACTGLTFTVGDARGDLYAFH
                     ARTMEFGPDVTGWRLISVPRGYSFKSCKMGICGDGVAGFEWKARYQHVGMSPARPGLP
                     VLDEVADGINEKGLACGCFYHMGYEEYNDKPRSDKAIANTDFVSWVLSNFATVREVRD
                     ALEQGRVDVAQFTLKIKGKLLCEPGTCPQAHYAVSDETGSSIVVEFKAGKAKIFDSVG
                     VITNNPEYDWHTTNLKNYIGLQTLSRDSAVFNGRTYDKLGNGTGSIGLPGDFTPPSRF
                     VRAMFFLNSAMPKVQTGPDEAIGRAFRILNQFDIPEGSIVAASPTGKGQIQDSTMWTA
                     LADLKRKRYYYHTQASRTVRMVELNRQPKNVLSVQDLPAEELILDVSNKFTPAGKTR"
     gene            complement(1939..4197)
                     /locus_tag="PLA58_06165"
     CDS             complement(1939..4197)
                     /locus_tag="PLA58_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOR62416.1"
                     /translation="MKPGTIHPSVMTMKIRILLFFLIIAAFLFTASNLQAGPAALDER
                     PRIGLVLSGGGARGLAHVGVIQVLEEMKIPISCIAGTSMGAIVGGIYASGMSPAELEK
                     FITSMQWNEAFKDKPDASEMTFRRKRDAADFLIDLDLGFKDGEFTMPRGVLQGQNLNL
                     ILKSMLIHTEGIDDFNRLNIPFRAVAADIETGDAVILEKGELVKALRASMSIPALFAP
                     VEMDGRLLVDGGIANNLPIDIARRMGADVVIVVDISTELSRRDQLATSVAITSQLTSI
                     MVQRNTQAQIRTMREGDILIRPEMGAIGSSDFFKAADAVAAGRKKAGEMKPELAHLAV
                     KEEEFLAYLQAQRKKEQPLPVIDVVEVDNKSSLPDALIAAHVDIKPGEPLSMPDLKKN
                     IDRVYGIDTFERVDFHVRKKDKSTAIVIEPFDKAWGPNYFRFGLGLEDNFKGSGSYAL
                     TAQFTKTALNRLAGEWRSEVRIGENPRIYTELYQPLDDSLSYFAVANAAYLMRNINTY
                     NDKGEILTQYRVQSAQFGLGAGRQFGNWGEVRVGLHREHGNVKIAIGDPSVPENIYNR
                     GSVQASVAYSRLDNYIFPLRGTDASLLWSYNLKALGSDIDEQALGLSWMTAFTWGKYT
                     FIPALDIKTMLDDDNMDVQDTFPLGGFLNLSGFSTNEIYGRHTGLARLVAYREIGSAG
                     MGALKMPLYIGLSAEAGNVWNKRSDINFESLILAGSIFLGIKTYLGPVYLAYGQAQRD
                     HSSIYLNVGQRF"
     gene            complement(4237..>4821)
                     /locus_tag="PLA58_06170"
     CDS             complement(4237..>4821)
                     /locus_tag="PLA58_06170"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOR62417.1"
                     /translation="FFMYYQILKAASDEIGWVWKGAGKQVRLERIYLPGREKMIQRIL
                     RDFPAVGKRPRKISGGLDRLMVDLCLGKERRVDLSLLNLSGLTKFSARVLKETLGIPR
                     GKVCAYSGLAERAGFPRAARAVGSVMANNPFPLAIPCHRVIRADGTTGHFGGGADMKK
                     RLLEREGIPFDAAGRVPPAYLVVVGSPRRANGRS"
     assembly_gap    4822..4921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4924..5475)
                     /locus_tag="PLA58_06175"
     CDS             complement(4924..5475)
                     /locus_tag="PLA58_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOR62418.1"
                     /translation="MATIFLLAFGLGVDAFSVAIGIGAAHHKKDWAPVLRLSFAFGIF
                     QFVMPLIGWLAGSTVVGLIRSFDHWIAFALLAAVGGKMIREGFEAESDEEKEDQTHGW
                     ALFLLSMATSIDALAVGFSFSLLKTPILFPSAVIGVVCFMMTAVGMVFGKTLAKIFGK
                     KVEIAGGLVLIGIGVKILIEHMR"
     gene            5754..7292
                     /locus_tag="PLA58_06180"
     CDS             5754..7292
                     /locus_tag="PLA58_06180"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOR62419.1"
                     /translation="MIKTVEQYLESLNDGREVWCLGEKVKDVRTHPSISTIVRMAAMD
                     YVLPNHPDYRELFVTKDADGEDIHFLLTSPKTPQDMLRRRECYMVGMRSGGGVLVHCM
                     GADALASFSVAAEVMDKKLGTHYVERVENYRRYLQKNDLALTGAITDVKGDRSLHPSQ
                     QKQHKDFYLRIVDRQKDGIVVRGAKVHISASPCANELLCLPCRTHGEADKDYALGFAV
                     PVAAKGVKLLGVEPNVRTYGEECEFDYPASDHLQPSESLIVFDDVFVPWERVFMCGEW
                     QYSQILAYAFASYHRLFGTCKMTWTLELMTGAASLIAEYNGVQNIPHVRKKLAWMAYI
                     TETVQMLAKQACLDPITEFGMDVMMPNRMAINASKWTYASNFHTMCQHMQDIGGGLTT
                     TVPAFRDWNNPEIQPFIDKYLAAKDGVSTEDRLRILRLIKDITGNHWQVDTIHGEGSM
                     AAQEMFLYGSANWKKLQAAARRAANMDGWQDDSVYGKLPTIAECVKMPPIDESYKSFA
                     PRTS"
     gene            7387..9948
                     /gene="hrpB"
                     /locus_tag="PLA58_06185"
     CDS             7387..9948
                     /gene="hrpB"
                     /locus_tag="PLA58_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HOR62420.1"
                     /translation="MQKDLKNGRILTSCPIDEILPELKEAIRKHSSVVLQAPPGAGKT
                     TRVPLALLDVIPPEKGRILMLEPRKLAAVSAARWMAKTLGEEIGQTLGYSIRFDSRVS
                     KETRIEVMTEGILTRRILANPDLAGVAMVIFDEFHERSLQTDLALALCLDVRRALRED
                     LKILVMSATLDGGPVAALLDGAPVISSRGKAFAVEERYCGDHPNQSVTARMTDAVERA
                     LQETSGDILAFLPGAGEILAVRNALEEKLRNRAMSNLSIHPLYGDLPFEEQERAILPA
                     SGRKIVLATNIAETSLTIEGVRVVIDCGLTRRLQYDPATGMNRLVTVTVSQASARQRQ
                     GRAGRLGPGVCYRLYSQHTFQSLIPFAPPEIAISDLSSLVLDLAAWGVGDPAALSWLD
                     APPPAAWRAARELLKGLSALDAKGSITPVGKAMARLPLHPRLGRLLLKAADMGFPSLG
                     ADLAALLSERDILRHSGSSLTPPPGWLDLSERLQLLQHWRQKKSLTAKADLPALRAVD
                     RSSQQLRRLTDKTWEVSGKETRDVIPRLLLAAFPDRIAKRREDGSGRYVMRSGRGVRL
                     SAKILPAGSPYIVAPIVDKGAKAEGKVQMAESLTEELILEELAGEIETLRRLYWNGQE
                     GRMIAVEEERLGTLTLSAKPISPSREEVATLLCEAIRSKTAMITFSREALQLQARVRL
                     MKETYPEENWPDLSPEALLASPADWLLPWLSGIRNAQQLACLNILPALTETLTWNQRR
                     LLHERAPATVAVPSGHNIPIDYVAGEIPVLAVKLQEMFGLADTPAIAGGRVKLLLHLL
                     SPARRPVQITRDLSQFWNTGYPQVKKELKGRYPKHPWPDDPWNAPPTRKTKGHKG"
     gene            complement(10017..11819)
                     /locus_tag="PLA58_06190"
     CDS             complement(10017..11819)
                     /locus_tag="PLA58_06190"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOR62421.1"
                     /translation="MDMPKRKMSRKRAGGHPLNLHFVYLLACVSLMLFLAGCESSGQN
                     RMIFYGGDIITMSDTPAGGIEAVFVENGKIVSTGTKDEILKHGTDGTRIINLEGKTLM
                     PGFIAVHTHPDLTAYLYGFVDLSGFTHKTKEEVWAKLRQTVRETKKGEWIFCKGFDPM
                     LVPGLTAPDIQFLDAVAPDHPVLILAQNLHSAWANSLAFKKMGVTATTPDPAPGSYFE
                     KDSQGRLTGFIVETGAVKVMSADVLKTFNIKDNAVKVADAHIGNGITSITTMGLFSDK
                     ALILYEHLSTAHPRLIHRVLGAIGVLPKRKPTVRHFMYILHSTPDLFPKNVENGDDFF
                     KICGMKIWYDGSPYTGSMYINEPYLNSELMLKGLKVPANHRGQTVIGKQEFYDAVKKY
                     HELGWQVSVHSQGDRASEEVLDVFDKVQSESSVKNLRHRLEHGVLLPPELLAKMKRLE
                     MTLSFHVNHLYYYGKALRDDILGAGRTARMLPVNSSRTAGLYYSLHADAPMYPEEPLS
                     MLQTAVIRKTREGEIIGPHEAVSVMEGLKALTIYAAWQLKMEKKLGSIEPGKYADFVI
                     LDRNPLKVNPDSLRDIRVLNTFVNGNEVWSRKTR"
     gene            complement(11881..12651)
                     /locus_tag="PLA58_06195"
     CDS             complement(11881..12651)
                     /locus_tag="PLA58_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOR62422.1"
                     /translation="MNWLEVSDELVKMLRLKTDPLAFKRFEKSEELEAIRNVFHIPTF
                     STFCQAVHRARVQGLTIGITKLDKMNHRCLRLHGVKHAHEKSMKEEAAMLSRTWFASP
                     EEALLQQQETPRVPVAEAVALAPLSKGKFDPDVVLFYGNPAQMMMLLCGLQKVKYERF
                     QFFFIGEGACADSLGECYRTGKPQLSIPCYGERAMGQVADDEISVALPPGEIPRAISG
                     MKQLAKIGFKYPINFIGGQADLEPILAQVYPDLKERKS"
     gene            complement(12691..13275)
                     /locus_tag="PLA58_06200"
     CDS             complement(12691..13275)
                     /locus_tag="PLA58_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOR62423.1"
                     /translation="MMNIGKHDALLVVDVQNDFLPGGALSMAGGDRIVPVINRISALF
                     TTRVFTRDWHPADHVSFSGHPQFLDQSWPPHCIQNTWGAQFHADLKIDAKDRIVSTGD
                     NPQEENYSSFHGTDLEAWLRRRDIRRIFIAGLATEYYVLTSAMDGLQAGFEVVVLEDA
                     VAGVDVPPGSAARALQKLRDSGVHILSSGDLLSE"
     gene            13505..14053
                     /locus_tag="PLA58_06205"
     CDS             13505..14053
                     /locus_tag="PLA58_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62424.1"
                     /translation="MVKKHRKDSKKRLASKHFNQGSGRASKINASTAYGTCSEQISPF
                     GGLLGLTKVLDLFDFEKHFNEGYHAPSRETQLGDYAMVMGLVMLLFIGFNRIWHFVYL
                     RLEPMLCGIFSVDKLPASSAYWRYLDSLGINQAQSLLRIMSRLREQAWKRCGIQYSYI
                     AIDIDATVENHLWRSGRRTGRP"
     gene            13998..14933
                     /locus_tag="PLA58_06210"
     CDS             13998..14933
                     /locus_tag="PLA58_06210"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOR62425.1"
                     /translation="MPPWKTIYGDQEGGRVGHNPKHRGKKGYRPILCFIEQPREYLYG
                     KLRKGETVSGEETAKVIRIIKDHLPSCVQKVLLRADAEFMSWNSVDAALDEGYDFIFS
                     NKQCEPLFDPKTWYRPSRKEEAEFNGCMYQPTGWGQACRFVAMRLPKKPEEIKSQPQQ
                     ELFEKDPYTYRIFCANLNGKAHEAIERYDKRADAENLIGEAKQEGLAAIPSGKFKNNY
                     AFFQIVLLAYNLWRYFKLLAGCCGKKVKILSGIADNKIRIGRLILLMIGAKLVKSGNR
                     DRVKYSIRDTRTPALLLFYEFLDHLRFKKIQECKA"
     gene            complement(15058..15582)
                     /locus_tag="PLA58_06215"
                     /pseudo
     CDS             complement(15058..15582)
                     /locus_tag="PLA58_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         3575 a         4408 c         4087 g         3515 t
ORIGIN      
        1 gagggcgccc tgcaaatcat cgacggcaag gccaaagtcg tttcggaaac attctgcgac
       61 ggtctgggcg cctgcatcgg agaatgtccg acgggtgcgc tgaaaatcgt tgagcgtaaa
      121 gcttctccgt ttgacgaaga ggctgtccgg aggcatctgt caaaaaaaga accggaaaca
      181 atgccgtgcg gatgtcccgc cgcgcacatt cagatttttg acaaacccat cgcctgtgaa
      241 gcggccaaca agccgaaaca ttttgaaagc gccgaggaag aatctgcgct ttcccactgg
      301 ccgattcaga ttaaactgat cccggcggga gccccctttc tgaaaggggc cgacctgctg
      361 gtcctcgcgg actgcacggc ggtcgccttc ccctcgctgc accggaatct attgaaaggc
      421 agggtggtga tgatgggctg tccgaaattc gacgatgtac aggaatacgt tcaaaagttt
      481 gccgacattt tccggacggc ggacatcaaa agcgttacgg tggccgtcat ggaagtaccc
      541 tgctgctcgg gcatgcccat gatcgtccga aaaggcatgg aagcggcaaa ccgaaaaatt
      601 cccctgaccg aaatcgtcct gagtttaagg gggaaaattc tgaaaaaaaa ctagtccgtc
      661 ggcagatccg gggcggagga ttcctgacga aagcagggat cctcctcccc ttttttttca
      721 gcgggttttg ccggccggtg taaacttatt tgatacgtca agaatcaatt cctcagcggg
      781 aaggtcctgg accgaaagaa cgtttttcgg ctgtcggttc agctcaacca ttcgcacggt
      841 tctgctcgcc tgagtgtgat aataataccg tttgcgcttc agatccgcca acgccgtcca
      901 catcgtcgaa tcctgaatct ggccttttcc ggtcggagac gccgcaacga tcgacccctc
      961 gggaatatcg aactggttca gaatccgaaa agccctgccg atcgcctcat ccggccccgt
     1021 ctgaaccttc ggcatggccg aattcagaaa aaacatggcc cggacaaacc gcgacggcgg
     1081 cgtgaagtcg cccggcaatc cgatggaacc ggttccgttt cccagtttgt cgtaagttct
     1141 gccgttgaag acggcgctgt ctctgcttaa cgtctgcagc ccgatataat tcttcagatt
     1201 ggtcgtatgc cagtcatact cgggattgtt ggtgatgacg ccgacactgt cgaagatttt
     1261 tgcctttccc gccttgaact cgacgacaat ggaagatccc gtttcgtccg acacggcata
     1321 gtgggcctgc gggcaggtgc ccggctcgca cagaagtttg cctttgatct ttaaggtgaa
     1381 ctgggcgaca tccaccctgc cctgctccag ggcgtcgcga acctcccgca cggtggcgaa
     1441 attgctcagc acccaggata cgaaatccgt attggcaatc gctttgtcgc ttcggggttt
     1501 gtcgttgtac tcctcgtacc ccatgtgata gaaacatcca caggccagtc ccttctcatt
     1561 gatgccgtcc gcgacctcgt ccagaacggg cagtcccggc cgggccggac tcatgcccac
     1621 gtgctgatat cgcgccttcc actcaaaccc cgcaacgccg tctccgcaaa tgcccatctt
     1681 gcagcttttg aaggaatagc ccctgggaac ggaaatcaga cgccagccgg tcacatccgg
     1741 cccgaactcc atcgttcgcg catgaaaagc gtacaagtct ccgcgcgcat ccccaacggt
     1801 gaacgtcaga ccggtgcagg ccccggcaac ggaaaaagct agaaacacgg atacgaccac
     1861 tgcaaataca accgttttga ttttaaaaag cattcggttt tcctcctttg taaattcagc
     1921 aagtgagtct cacgccgttt aaaaccgctg gcccacattg agataaatgg acgaatggtc
     1981 ccgctgggcc tggccatagg ccaggtaaac cggcccgagg tacgttttga ttcccagaaa
     2041 aatgctcccc gccagaatca gggactcgaa attgatgtcc gaccgtttgt tccagacatt
     2101 gcccgcctcc gccgacaggc cgatgtacag gggcatcttc agcgcgccca tgcccgcaga
     2161 gccgatttcc cggtaggcca ccagccgggc caggccggta tggcgtccgt agatttcatt
     2221 ggtggaaaag ccggacagat tcaggaagcc gcccagcgga aacgtgtcct gcacatccat
     2281 gttgtcgtca tccagcatgg ttttgatatc gagcgcgggg ataaaggtgt acttccccca
     2341 ggtgaaggcg gtcatccagc tcaatcccag ggcctgttca tcaatgtccg aacccaacgc
     2401 cttcaggttg taactccaca gcaggctggc gtccgttccc ctgagcggga aaatataatt
     2461 gtccagcctg ctgtacgcga cggaagcctg aacgctgccg cggttataga tattttccgg
     2521 cacactgggg tcgccgatgg caatcttcac gttaccgtgc tcccggtgaa gtcccacacg
     2581 cacttcaccc caattgccga attgacggcc tgctcccaaa ccgaattgag cggactgaac
     2641 gcggtactgc gtcaggatct cgcccttatc gttgtaggtg ttgatgttgc gcatgaggta
     2701 ggcggcattg gccacggcaa aataactgag ggaatcgtcc agcggctggt aaagttcggt
     2761 atagatgcgc gggttctcgc cgattctcac ttcgctgcgc cattctcccg ccagacggtt
     2821 cagggccgtc ttggtaaact gcgccgtcag ggcgtaactg ccggatccct tgaagttgtc
     2881 ctccaggcca aggccgaacc ggaaataatt cggcccccat gccttatcga aaggttcgat
     2941 gacaatcgcc gtggacttgt cttttttccg aacgtggaag tccacgcgtt caaaggtatc
     3001 gatgccgtaa acccggtcga tatttttctt caagtcgggc atgcttaggg gctcacccgg
     3061 tttgatgtcc acgtgcgcgg caatcagcgc gtctggcaac gacgacttgt tgtccacctc
     3121 cacaacgtcg atcaccggca gcggctgttc ttttttccgc tgcgcctgca gataggcgag
     3181 aaattcctct tccttcacgg ccagatgcgc gagctcgggt ttcatctccc cggctttctt
     3241 ccgtccggcg gcgacggcgt ccgccgcctt gaagaaatca ctactgccga tggcgcccat
     3301 ctccggacga atcaggatgt ccccttcccg catggtgcga atctgcgcct gggtgttgcg
     3361 ctgaaccatg atggaggtca gctgagaggt gatggccacg gaggttgcca actgatcacg
     3421 gcggctcagc tccgtgctga tgtccacgac aatcacgaca tccgcgccca tccgccgtgc
     3481 gatatcgatc ggcagattgt tggcaatgcc gccgtccacc agaagcctgc cgtccatttc
     3541 caccggggcg aacagcgcgg gaatggacat actggcgcga agcgccttca ccagctcccc
     3601 tttttccaga atgacggcgt cgccggtttc gatatccgcg gccacggccc gaaacggaat
     3661 gttcagccgg ttaaaatcat caatgccctc cgtgtgaatc agcatggatt tcagaatcag
     3721 gttcagattc tgcccctgaa gcactcctct gggcatggta aattctccgt ctttgaaacc
     3781 cagatcgagg tcgatcagaa agtcggcagc atccctcttc cggcggaagg tcatttcgct
     3841 cgcatccggc ttatctttga aggcttcatt ccactgcatg ctggtaatga acttttccag
     3901 ttcggcaggc gacatgccgg aggcgtaaat tccgccgaca atcgccccca tgctggttcc
     3961 cgcgatgcag gaaatcggaa ttttcatttc ctccagaacc tgaatcacac ccacgtgcgc
     4021 cagcccccgt gcgccgccgc cgctcagcac cagaccgatc cggggacgtt cgtccaaagc
     4081 ggccggcccg gcctgaaggt tggacgccgt gaagagaaaa gcggcgatga tcaggaagaa
     4141 tagcagaatt ctaatcttca tggtcatgac ggaaggatgg atggttcccg gtttcatgaa
     4201 tgtcccctcc gattgccgtc gttgaaaatg gaagattcag gagcgcccat ttgcccgccg
     4261 cggcgatccc accactacga gataggccgg aggcactctt ccggccgcgt caaacggaat
     4321 tccttcccgt tccagcaggc gctttttcat gtcggcgccg ccgccaaaat gaccggtcgt
     4381 cccgtcggcc cgaatcacgc gatggcaggg aatggcaagc ggaaaaggat tgttcgccat
     4441 cacggaaccc accgcccggg ccgcgcgtgg aaaaccggcc ctttccgcca gacccgaata
     4501 ggcacagact ttcccgcggg ggatgccgag cgtttccttt aacacccgcg ccgagaattt
     4561 cgtcaacccg gataaattca gcaacgatag atccacgcgc cgttctttcc caaggcacaa
     4621 atcaaccatc agacgatcca gaccgccgga tattttgcgc ggccttttcc cgacagcggg
     4681 aaaatcccgg aggattctct ggatcatctt ttccctgccc ggaagataaa tcctttccag
     4741 ccggacctgc tttccggccc ccttccacac ccatccgatt tcgtcgcttg ccgccttcag
     4801 gatttgataa tacataaaaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 ncttcacctc atatgttcga tcaatatctt cacaccgatg ccgatcagga ccagtcctcc
     4981 cgcaatttcc acttttttgc cgaagatttt tgcgagcgtt ttcccgaata ccatcccaac
     5041 cgccgtcatc atgaagcaaa ccacaccgat caccgccgac ggaaacagaa tgggcgtctt
     5101 cagaagagag aaactgaatc ccaccgccag cgcatcgatg ctggtggcca tcgaaagcag
     5161 aaaaagcgcc cagccgtgcg tctgatcctc tttttcctca tcgctttccg cttcaaatcc
     5221 ttcccggatc atttttccgc cgaccgccgc caaaagcgcg aaagcaatcc agtgatcgaa
     5281 ggaccggatc agtccgacca cggtcgaacc ggccagccag ccgattaacg gcatcacaaa
     5341 ctgaaaaatg ccgaatgcaa aggacagacg caaaaccggc gcccagtcct ttttatgatg
     5401 ggccgcaccg atgccgatcg ccacggaaaa cgcgtccacg cccagaccga acgccagaag
     5461 aaatatggtt gccaagctca tctgcagcaa cactctctcc aacagaattc ttggcggatt
     5521 ctatagccac aggacaggcg aaaagtaaat cctgaatctt gcgcgcattg aaggatttcg
     5581 tgatcagcga aaaaagattt cacatcatta ttctcctgta gcttttcaag gctaaatccc
     5641 tgcaagaaat ttcttgtaag gaaggggtca ttaaattgtt tattattaat gtttttcctg
     5701 atattcttcc tgcaatttat tctggagata aaaatagata aggagagggg atcatgatca
     5761 agaccgtgga gcaatatctg gaaagtttga atgacggccg ggaagtctgg tgtctgggcg
     5821 aaaaggtaaa agatgtccgg acccatccgt cgatcagcac gattgtccgg atggctgcga
     5881 tggattacgt tttgccgaat catccggatt accgggaatt gtttgtcaca aaggatgcag
     5941 acggcgagga catccatttt ctgctcacct ctccgaaaac accgcaggat atgctgcgca
     6001 ggcgcgaatg ctacatggtc ggcatgcggt cgggcggcgg cgtgctcgtg cactgcatgg
     6061 gcgcggatgc cctggcttcc ttcagtgtgg ccgccgaggt gatggataag aaactcggca
     6121 cccattatgt cgaacgcgtc gaaaattatc gccgctacct ccaaaaaaac gaccttgccc
     6181 tgaccggcgc catcacggat gtcaagggag accgcagtct tcacccctcg cagcagaaac
     6241 agcacaagga tttttacctg agaatcgtcg accggcagaa agacggcatt gtcgtgcggg
     6301 gcgccaaagt ccatatcagc gcgtctccct gcgccaacga gcttttgtgc ctgccctgcc
     6361 ggacccacgg cgaagcggac aaggactacg cgctgggttt tgcggtgccg gttgccgcaa
     6421 aaggcgtcaa acttctgggc gtggagccga atgtgcggac gtacggagaa gaatgcgaat
     6481 tcgattatcc cgcttccgat cacctccagc cttcggaatc cctgatcgtt tttgacgatg
     6541 tgtttgtacc ctgggagcgg gtttttatgt gcggcgaatg gcagtactcc cagattctgg
     6601 cgtatgcctt cgccagctat caccgtctgt tcggcacctg caagatgacg tggacgctgg
     6661 agctgatgac gggcgcggcg agtctcattg ccgaatacaa cggcgtccag aatattcccc
     6721 atgtgcgcaa gaagctggcc tggatggcct atatcacgga aaccgtccag atgctggcca
     6781 agcaggcctg ccttgacccg atcactgagt tcggcatgga tgtcatgatg cccaaccgca
     6841 tggcgatcaa cgcctccaag tggacgtatg ccagcaactt ccacaccatg tgccagcaca
     6901 tgcaggacat cggcggcgga ctcaccacca ccgtcccggc gtttcgcgac tggaacaatc
     6961 cggaaatcca gccgtttatc gacaaatatc tggcggccaa agacggcgtt tccactgaag
     7021 accgtctgcg cattttaaga ctgatcaagg acatcaccgg aaatcactgg caggttgata
     7081 ccattcacgg cgaaggatcg atggccgctc aggaaatgtt tctttacggc agcgccaact
     7141 ggaaaaagct tcaggcggcg gcccgtcgcg ccgccaacat ggacggctgg caagatgatt
     7201 ccgtttacgg caagttgccg accatcgccg aatgcgtcaa gatgccgccc attgacgaat
     7261 cgtataagag cttcgcgccc cggacaagtt aatgatgtcg cgtatcgggt ggagcatccg
     7321 gtcatatcca tcggggaaat ccggtttgta ttgacatgat cgcccgcctt ccctatactt
     7381 tgcggcatgc agaaggatct gaagaacgga cgtattttga catcctgccc cattgatgag
     7441 attctgccgg agttgaaaga agccatccgg aagcattctt ccgtggttct tcaagcgccg
     7501 cccggcgccg gcaagaccac acgggttccg ttggcgctcc tggatgtgat cccgcctgaa
     7561 aaggggcgaa tcctcatgct ggaaccccga aagcttgccg ccgtctccgc agcccggtgg
     7621 atggcgaaga cccttggcga ggaaatcggt cagaccctgg ggtactccat ccggtttgac
     7681 agccgcgtct cgaaagaaac ccgcattgaa gtcatgacgg aagggattct caccaggcgc
     7741 atcctggcca atcctgatct tgcgggcgtc gccatggtta tttttgacga attccatgag
     7801 cggagcttgc aaaccgacct ggcgctcgct ctttgtctgg atgtccgccg cgccctgcgg
     7861 gaagatctga aaattcttgt gatgtccgcc acactcgacg gcggtccggt tgcggccctt
     7921 ctcgacgggg cgcccgtcat ttcatcgcgg ggcaaagcct tcgctgtgga agagcgctac
     7981 tgcggagacc atccgaatca atccgttacg gcacgcatga cggatgctgt tgagagggct
     8041 ttacaggaaa cgtccggcga cattcttgcc tttctgccgg gagccggcga aattctcgcc
     8101 gtgcgcaatg ccctcgaaga aaagctcagg aacagggcaa tgagcaacct gtcgatccat
     8161 cccctttacg gtgatttgcc ctttgaagag caggaacggg ccatccttcc cgcgtccggg
     8221 cgcaagatcg tcctcgcgac caacatcgcg gaaaccagcc tgaccattga aggcgtccgc
     8281 gtggtgattg actgcggatt gacccgcagg cttcagtacg atccggcaac cggcatgaac
     8341 cgtctggtca ccgtcacggt ttcacaggct tcggcccggc agcgtcaggg acgcgccggc
     8401 cgcctcgggc cgggcgtctg ctatcgtctc tacagtcaac acaccttcca atccctgatt
     8461 ccctttgccc cgccggaaat cgccatttcc gatttatcgt ctctggtttt ggatctggcc
     8521 gcctggggcg tcggggaccc ggcggcgctt tcctggcttg atgcgcctcc gcccgctgcc
     8581 tggcgcgcgg cgcgggagct gctcaaagga ttgagcgcgc tggatgcaaa aggttcgatt
     8641 acaccggtcg gcaaggccat ggcgcgcctg ccgcttcatc cgcgcctggg ccgcctgctt
     8701 ctgaaagcgg ctgacatggg cttcccgtcc ctcggcgcgg atctggcggc tcttctgtct
     8761 gaaagggaca tcctgcgcca ctccggatct tccctgacgc ccccacccgg ttggcttgat
     8821 ttgagcgaac gtctccaact gctgcagcac tggcgacaga aaaagagtct gaccgcaaaa
     8881 gccgaccttc cggccctgcg cgctgttgac cgatcgtcgc agcaattgag gcgactgacg
     8941 gacaaaacat gggaggtctc cgggaaagag acaagggacg tgatcccccg tttgcttctg
     9001 gctgcttttc ccgaccgcat tgccaaaaga cgcgaagacg gcagcggccg ttatgtcatg
     9061 cgttcgggaa gaggcgtccg gctgtccgca aaaatcctcc cggccggaag tccctatatt
     9121 gttgcgccca tagtggacaa gggcgcaaag gcggaaggca aagtgcaaat ggccgaatcg
     9181 ctcacggagg agctcatcct ggaggaattg gcaggcgaga tcgaaaccct gcggcgcctt
     9241 tactggaacg gacaggaagg tcggatgatc gctgttgagg aagagcgcct ggggacactc
     9301 acgctttccg ccaaaccgat aagcccgtcc agggaggaag tcgcgacgct gctttgtgaa
     9361 gccatccggt caaagacggc catgatcacc ttcagccggg aggccttgca gcttcaggcc
     9421 cgtgtgcgcc tgatgaaaga aacgtatccc gaagaaaact ggccggacct ttctcctgaa
     9481 gcgcttctgg cgtcgccagc agactggctt ttgccctggc tttccgggat ccgcaacgcc
     9541 cagcagctcg cctgcctgaa catcctgccg gcgcttacgg aaacgctgac gtggaaccag
     9601 cggcgtcttc tccatgagcg cgcccctgcg actgttgccg tacccagcgg ccacaacatt
     9661 ccgattgatt atgttgcagg cgaaattccg gtcctggccg tcaaactcca ggagatgttc
     9721 ggtctggccg acaccccggc catagcaggc ggccgtgtaa aattgctgct tcaccttctc
     9781 tcgcccgccc ggagacccgt tcagatcacc cgggatctga gccaattctg gaacaccggc
     9841 tacccgcagg tgaaaaaaga gttgaagggc cgctatccga aacatccctg gcccgacgac
     9901 ccctggaacg ctcctccgac aagaaaaaca aaagggcaca aaggatgacg gaaacaggat
     9961 tttaggcagc aggccgtgaa aaattcatcc cggttttccc tcgtctcgtt attttttcaa
    10021 cgtgttttcc ggctccaaac ttcattgcca ttgacaaagg tattcagcac acggatgtcc
    10081 ctcagggagt cagggttgac tttaagaggg ttcctgtcga ggataacaaa atcggcatac
    10141 ttaccgggtt cgatactacc cagctttttt tccatcttga gctgccaggc ggcataaatg
    10201 gtcagcgctt ttaatccttc cattacggag acagcctcat gaggcccgat gatttctccc
    10261 tcgcgggttt tccgtatcac agccgtctgg agcatgctta ggggttcttc cggatacatg
    10321 ggggcatcgg cgtgcaaaga ataatacagc cctgccgtcc tcgagctgtt caccggcaac
    10381 attcttgcag tccgcccggc gcctaaaata tcatctctca aagctttacc gtaataataa
    10441 agatggttaa catggaagct gagggtcatc tcaagccgct tcattttcgc cagcagttcc
    10501 gggggcagga ggacgccgtg ttcaaggcgg tgccgtaagt ttttcaccga tgattccgac
    10561 tggaccttat caaaaacatc cagaacctct tcactggccc gatcgccctg tgagtgcaca
    10621 ctgacctgcc agccgagttc atgatatttt ttgacggcgt cataaaattc ctgcttgccg
    10681 atgacggttt gtccgcgatg attggcggga actttcagcc ctttcagcat cagttcggaa
    10741 ttcaggtaag gctcattgat atacatggag cctgtatagg gggagccgtc gtaccagatt
    10801 ttcataccgc agatcttgaa aaagtcatct ccgttttcaa catttttcgg aaacagatcg
    10861 ggggtcgaat gcaggatgta cataaaatgc cgcacagtgg gtttgcgctt gggcagcaca
    10921 ccaatcgcac ccagcacccg gtgaatgagc ctcgggtgag ctgtggatag gtgttcatat
    10981 aagatcaggg ccttgtcgct gaagagcccc atggttgtaa tgctcgttat cccattgcca
    11041 atgtgtgcgt cggcgacttt aacggcattg tccttgatgt taaatgtttt gagtacgtcg
    11101 gcggacatca cttttacggc tccggtttca acaataaaac cggtaagcct gccctgactg
    11161 tctttttcaa agtaactccc gggagccggg tcgggtgttg tcgccgtgac gcccattttt
    11221 ttaaaggcca gagaattggc ccatgccgag tgtaagttct gggcgaggat gagcaccggg
    11281 tgatccgggg cgacggcatc cagaaactga atatcgggag ccgtgagtcc cgggaccagc
    11341 atcgggtcaa atcccttgca aaaaatccat tcccctttct tggtttccct caccgtctga
    11401 cgcagctttg cccacacctc ttcctttgtt ttatgggtaa agccgctgag atcgacaaaa
    11461 ccatacagat atgccgtaag atccggatgg gtgtggacgg caataaatcc cggcatcaat
    11521 gttttcccct ccagattgat gatccgtgtg ccgtctgtcc catgcttgag aatctcgtct
    11581 tttgtcccgg tcgaaacaat tttaccgttt tccacgaata cggcttcaat acccccggca
    11641 ggcgtgtcgc tcatggtaat gatgtcaccc ccgtaaaaga tcatccggtt ttgtccggac
    11701 gactcgcagc cggccagaaa caacatcagc gaaacgcagg ccaaaagata cacgaaatga
    11761 aggtttaacg ggtgaccgcc tgctctcttc cgggacattt tccttttcgg catatccatg
    11821 gcttcaatcc tccctcgtgt tgttgattgc ttgataatct actctatctc tttttccgtt
    11881 ctatgatttc ctttctttta agtccggata gacctgagcc agaatcggct ccaggtcggc
    11941 ctggccgccg atgaaattga tcggatattt gaagccaatt ttagccagtt gtttcattcc
    12001 tgagatggcc ctggggatct ctccgggcgg gagagccacg gatatttcat cgtcggccac
    12061 ctgccccatg gccctttctc cgtaacaggg aatggacaat tgaggcttgc cggtccggta
    12121 gcattccccc agcgaatcgg cgcaggcccc ctcgccgata aagaaaaact gaaagcgctc
    12181 atattttacc ttctgcaatc cgcacaggag catcatcatc tgcgcgggat ttccgtaaaa
    12241 gagaacgaca tccgggtcga actttccctt cgacagaggt gccagcgcaa cggcttcagc
    12301 caccggaacg cgaggggttt cctgctgctg caacaaagct tcttcgggcg aggcaaacca
    12361 tgtacgtgag agcatggccg cctcttcttt catgctcttc tcatgcgcgt gtttgacgcc
    12421 gtgcaggcgc agacagcggt gattcatctt atcgagcttt gtgatcccga tggtcagacc
    12481 ctgaactctg gcgcggtgca cagcctggca gaaggtggag aatgtcggga tgtggaatac
    12541 attcctgatc gcttccagct cctctgattt ttcaaagcgc ttgaaggcca gcggatctgt
    12601 tttcagccgc agcatcttaa ccagttcgtc gcttacttcc agccagttca tcaccaaacc
    12661 cttcctgtaa gaaatttgag acgggtgcca ttactccgac aacaaatcgc cggatgaaag
    12721 gatatgaacg cctgaatcgc gaagcttttg cagagccctg gccgcgcttc ccggaggaac
    12781 atccaccccc gcaacggcat cttcaagaac caccacttca aaaccggcct gcagaccgtc
    12841 catggcggag gtcaagacat aatattccgt ggccagaccg gcgataaaaa tccggcgaat
    12901 gtcccggcgg cgaagccacg cttccagatc tgtgccgtga aagctggagt agttttcttc
    12961 ctgcgggttg tcccccgtgc tgacaatccg gtcttttgcg tcaattttca agtcggcatg
    13021 aaactgtgcg ccccaggtgt tttgaataca atggggaggc caggattgat caagaaattg
    13081 cggatgaccg gaaaaactga catgatcggc cggatgccag tcgcgggtaa agacccgcgt
    13141 tgtaaaaagc gctgaaatcc ggttgatgac cggcacaatc cggtctcctc ccgccatgct
    13201 aagcgcgccg ccggggagaa aatcattttg cacgtcaacg acaagtaatg cgtcatgttt
    13261 gccaatgttc atcatgtgtc ccaccttcct gaaaatccat ccaccgcata tccaacatgg
    13321 ttatacgctg aaggagattg ccgatcatct ggagattcat gacacaactt tgagcactcc
    13381 gcctgaggaa aaactaatac ctgaaccctg cgcgacatag tgaaaaaaaa taaataaacc
    13441 atctgggaaa cctgttgtat aaagggatac gcaaataacc caatagcaaa caggaggtgc
    13501 ccagatggta aaaaagcata ggaaggatag caaaaaaagg cttgccagta aacattttaa
    13561 tcaggggagc gggagggcca gtaaaatcaa tgcttcgaca gcgtatggga catgtagcga
    13621 gcagataagc ccgtttggcg ggcttttggg actgaccaag gttttggact tatttgattt
    13681 tgagaaacat tttaatgagg gctaccatgc gccgtcgcgg gaaacccagc tgggcgacta
    13741 cgccatggtc atggggcttg tgatgctact gtttatcggg ttcaaccgga tctggcattt
    13801 tgtttatctc cgtttggagc ccatgctttg cggcatcttc agcgtggata aacttccggc
    13861 gtccagcgcc tattggcgtt acttggacag tctggggatc aatcaggcgc agtccctttt
    13921 gagaatcatg agccgtttga gagagcaggc ctggaaacga tgcggaattc aatacagcta
    13981 cattgctatc gatatcgatg ccaccgtgga aaaccatcta tggcgatcag gaaggcggac
    14041 gggtcggcca taatccgaag caccggggca agaaaggcta ccgaccgatt ttgtgcttta
    14101 ttgaacaacc ccgggaatac ctgtatggga aactgcgcaa aggggaaacc gtcagcgggg
    14161 aggagacggc caaagtgatt cggatcatta aagatcattt gccgtcctgt gtccaaaagg
    14221 ttcttttgag agcggacgct gagttcatga gttggaatag tgtcgacgcc gcattggatg
    14281 aggggtatga ttttatcttt tccaataagc aatgcgaacc gctgtttgac ccaaagacct
    14341 ggtatcgccc ctcccggaaa gaagaagccg agttcaacgg ctgcatgtat cagccgacgg
    14401 gttggggcca ggcatgccga tttgttgcca tgcggcttcc caagaaaccg gaggaaataa
    14461 aaagccagcc ccagcaggaa ctgtttgaaa aagatccgta cacctaccgg atattttgcg
    14521 ccaatctcaa cggaaaggcc catgaggcga ttgaacgata cgataagcga gcggatgcag
    14581 aaaacctgat tggtgaagcc aagcaggaag gcttggctgc gattccgtcc ggcaagttca
    14641 aaaacaacta tgctttcttt cagattgttc tgctggcgta taacctttgg cggtatttta
    14701 agctcctggc tggctgctgt gggaaaaagg taaaaatact ttctggcatc gctgacaaca
    14761 aaatcagaat tgggcgcctg atactgctga tgatcggcgc gaagctggtc aaatccggca
    14821 atcgtgaccg ggtgaaatat tcgatccgcg atacacggac gcctgcgttg ctattgtttt
    14881 atgaatttct ggatcatctc cgattcaaaa aaattcagga gtgcaaggca tgaatatttt
    14941 aactgatttg atgcaagaaa tttcttgcat gggcgccgtc ctgaaccgcc gtggcggata
    15001 gttcatgaaa ttttgctcag agattacaaa aatgctgaat gcgcgcagga ttcaggtgcc
    15061 aacataaaag gtgtcctggc tgattaaata ccccggatga gccgtctcga tctcgtcagg
    15121 tgaactctcc ctctctcgct tggcgttttc caatgccgat aactgtgctt ctgtataaac
    15181 aatgccttcc ctggcggctt tctcctctaa acaacataaa cgcttcttaa aggtctccag
    15241 accgtggcgc agccaaatac ttcgaacgcc accagatgaa attaaaatcc caatttttcg
    15301 taattcattg gacgttcttg cttgtccata agccgggtat tcataggcca tccggatgac
    15361 ggattcttca acctccggcg ctacccgatt ctttatacag ggcttccttc tgcttttctc
    15421 ccgaaggcca tccagtcctt cttcctcata ggctttcttg atgtcataaa aatgctgcct
    15481 cgatacacca ttgattttac aggcttctga aacgttcttc aaatactccg ccaactctag
    15541 caaactcaat ttgtttctga taagcttctc ttgggtggtc atttttaaat atcctccttt
    15601 tttgattgcc gcaaaacaat ccaggaggta actgaccacc ctttctacct aactgtcaag
    15661 tcaagtccgt cttcttacag ataaa
//
LOCUS       DAOSRM010000050        15473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_3922_length_15473_cov_118.592749, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000050 DAOSRM010000000
VERSION     DAOSRM010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15473
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3922_length_15473_cov_118.592749"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..288
                     /gene="rpsO"
                     /locus_tag="PLA58_06220"
     CDS             22..288
                     /gene="rpsO"
                     /locus_tag="PLA58_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOR62426.1"
                     /translation="MLTLEKRKALIEDYRLHEKDTGSPEVQIALLSARIEYLTEHFKT
                     HQKDHHSRRGLLKLVGQRRRLLNYIKEDDVERYRNIIKRLGLRK"
     gene            430..2550
                     /locus_tag="PLA58_06225"
     CDS             430..2550
                     /locus_tag="PLA58_06225"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOR62427.1"
                     /translation="MSDNFSTDFAGRNFILKTNYVAAQADGSALVYYGDTVVLVTAVS
                     LKSVREGVDFLPLTVDYQEMTFAAGKIPGGFFKREGRPNEREILTSRIIDRAIRPLFP
                     KGYYSETQLVATVLSVDKENDADVAAMIGASAALEISDIPFKGPIAGVRVGRIGGELV
                     ANASPEKMQESEMNLFLVGRKVTPSRSGRAYDVELVMMEGEAREVPEDIIVDAIKFGL
                     EAVRPVIDLQDRVRQAVGKAKRSVEEAAPDEELIARVREEALPGLQEGYSMPRKLERY
                     GKLGDVRSAVVKKIGGDDAALCKKVAAIIEHLESRILRDMIIQEKRRIDGRSSTDIRP
                     IHSEVGVLPRAHGSALFNRGETQALAALTLGTSSDEQRMDYVSGEELRSFLLHYNFPP
                     YSVGEAKSLRSPGRREIGHGALARKALIPVLPPPEQFPYTIRIVSEILSSNGSSSMAT
                     VCGGILCLMDGGVPVKDIVAGIAMGLLKEGEDVVILSDILGDEDHAGDMDFKVCGTEK
                     GVTAMQMDIKIDGLTEDILRKALAQAREGRLHIIGKIRETLSAPRSDISLYAPRITTV
                     KVKEDQVRTVIGSGGKNIRQIISETGVTIDVEDDGTVTIASADAEAAARAVAMVKWLT
                     EEAEVGKIYRGTVKKIVDFGAFVEILPGTEGLLHISQIAKERIAKVTDVLQEGDEVMV
                     KVLEVDKSGKIRLSRKEALGAEVK"
     gene            2622..3065
                     /gene="dut"
                     /locus_tag="PLA58_06230"
     CDS             2622..3065
                     /gene="dut"
                     /locus_tag="PLA58_06230"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOR62428.1"
                     /translation="METINVQIRKIAGNEDLPLPEYMTRLAAGMDLFAAVAAEETILP
                     GARKKIPTGIVISLPEGYEAQIRPRSGLALHQGLTLLNSPGTIDADYRGEIALIVINH
                     GDAPFVVKRGARLAQMVVQKICRVQWVEALQLDETPRGEGGFGHT"
     gene            3142..4320
                     /locus_tag="PLA58_06235"
     CDS             3142..4320
                     /locus_tag="PLA58_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOR62429.1"
                     /translation="MKKFKVKKTFQEINEKIKKGRAVVVTAEEMIDVVERHGDVEAAR
                     RVDVVTTGTFGAMCSSGAFLNFGHTAPRIRASRVWLNDVPAYGGVAAVDCYIGATEVV
                     EGDPLNSVYPGEFNYGGGHVIEDLTAGNVVKLRAEGYGTDCYPSRKFEKNITINDLRD
                     AFLFNPRNAYQNYNCAVNLSDTTIYTYMGMLRPRMANVSYSTSGQLSPLFNDPYYRTI
                     GIGTRIFLGGAQGFVAWPGTQHNPHAARSANGVPKEGAGTLAVIGNLKEMSPEWLTGA
                     SILGYGVSLFVGIGIPIPILDEEMARYTAVKDEDIYAQVYDYSMDYPKGNPRPVAEVN
                     YRDLRSGTVIINDKKVIAAPLSSYYKAHQIAELLKGWIEAGKFLLGEPQQNLPSIPFP
                     "
     gene            4458..4724
                     /locus_tag="PLA58_06240"
     CDS             4458..4724
                     /locus_tag="PLA58_06240"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOR62430.1"
                     /translation="MKIGRYLVAFVFFMMLLIAFGNRGVVDNYFIAKRLSQLKAENNA
                     LIAQNKELAGKILLLRSDPAYIESIARNELGMVKPGDVVYRWTQ"
     gene            4758..5831
                     /gene="pilM"
                     /locus_tag="PLA58_06245"
     CDS             4758..5831
                     /gene="pilM"
                     /locus_tag="PLA58_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOR62431.1"
                     /translation="MDFKNLFSQTKSLVGLDIGSTSMKLAEIVSTSQGMTLNRFLQIP
                     VPPGVIVEGVLHDPDALAGVIKELFRNSGCKGKGIVTSLSGNSVIIKKVTLAQMEEAE
                     LRELIHDEAGKYLPFDNMDDVNYDFQILGDNEFNPNQMEVIIVAAKKTDVNGYLDAFA
                     AAGLQVMIMDVDSFALETMYEANYDFEENEIVVIVNIGASLTNINVIRGGMSVFTRDF
                     TMAGNAVTEALQERFQVTAEEAERMKVEGTAGAQEIPGLENAILDAAEPICSEIERSV
                     DYFRSTFGGEEIKHVFLSGGSSRIAGLSDLLSQRLNIGTQLINPLAKIGHNKKNIGEA
                     ELDGIKTIGAVAIGLGLRKIGDK"
     gene            5828..6376
                     /locus_tag="PLA58_06250"
     CDS             5828..6376
                     /locus_tag="PLA58_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOR62432.1"
                     /translation="MIKINLIPYHEKEKKENLARQIAVMAGAFLLFILCLAGVYVYMT
                     VQVGRLETKLTESRDKLKALEAKVGDLDKYKSQIAEFELKLGVIGTLEENRVYPVKMF
                     DDLAMLVPQKSVWLTKIGQSDESLTLEGVGRDNIAVAGFMKTIENFPAIQSVDLVSSK
                     KVEIAETTLQQFIFSCKLKKGF"
     gene            6386..7138
                     /gene="pilO"
                     /locus_tag="PLA58_06255"
     CDS             6386..7138
                     /gene="pilO"
                     /locus_tag="PLA58_06255"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOR62433.1"
                     /translation="MEFNLDELKKLSPQVKALIVVVVIFLIGYLYYFYFLSDTLTKRS
                     QMSKELEELQTQIQQKENIARQMNKVKAQVATLKENYQAALQKLPSRKEIPGLFYSVA
                     TAGREAGVEFVLFEPLPSVPQTLEKAEKLSGKLKPSDKRQEEQEKKAEAEKAAGKPPG
                     DAKKAPPAKAPASEPFYEEIPMKVTVTGGYQNIVFFFDKLARLPRIVNISDVSMGDRK
                     EGKGKKYSITASCIVKTYMFVDKKEQANEKKK"
     gene            7122..7685
                     /locus_tag="PLA58_06260"
     CDS             7122..7685
                     /locus_tag="PLA58_06260"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOR62434.1"
                     /translation="MKRKNKKQAIVWFGVWLFLATMLGACGDQEKAPAAPRPPAKPQA
                     VQTAPSVQPKTVQAAPASSAIPAVQMPANVYSYRPQGKPDPFKPFVDAQIVAAKKGKA
                     AATPSIFPLQKAGVENFNVVGIMGDDIRRMAIVEDAAKKFYPLFVGTRIGLHNGKVTD
                     ILADRVIVDELDGKKTRRVVLKLRQNQ"
     gene            7698..9392
                     /locus_tag="PLA58_06265"
     CDS             7698..9392
                     /locus_tag="PLA58_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HOR62435.1"
                     /translation="MRKYLSRIIPVVIVLWVFLYTPAGVMTGSGAQDNVGYLENILLE
                     KQPGREKVILEVSQQPANIPASLQGDNSLLIRLENLYLPNHLRETLRGNLLNVIDVRA
                     AQKTENGKPWVYLTIRLKENTPYSVVPEEKKIVVDFNISGLSSKLVQPRETAAGEKQA
                     AQVGGVKRYTDRIISLDFQDADIKSVLRLMAEYGNISIISGDDVKGNITLTLKSVPWD
                     QALDSILNIKGLTKKQSDDVIIVMTLAKKKQDEADRQAAFEAQQKADEERKAREQKSD
                     IEKGKLRQIMIEAKIVEASDDFIRNLGIQWGGGSSQRVGGTYGLGISGATNPLTTTTR
                     MNYAFPPEISPAMTMAAVNMPSAVAGPTLGLVFGGAHGFIEAQLQALEKTTRGKIISS
                     PRVVTMDDVKAIIKQGEEVPYITPATTSAPPTVAFKDALLKLEVKPKITDDNRISMDI
                     KASNDWADFTKANAQGENPPIVKNEVESKVVVKDGDTVVIGGIAKMDENRIAAGVPWF
                     YKIPVLGWLFKTENVTIKKRQLMVFITPKILTESGYNEDECDPTKMDNLDRCRNRK"
     gene            9506..10267
                     /locus_tag="PLA58_06270"
     CDS             9506..10267
                     /locus_tag="PLA58_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR62436.1"
                     /translation="MKLKTVLVFFLLAGALFFWSCTSTPYHRDQADIHLQKGIAFIQA
                     GKYIGALAELLEAEKNAPDDPEIRYYLGIAYLGRGMKDQAMEQFQKAVSLKENYSEAH
                     NYIGVMRMDEGQWQKAIESFDRALKNPVYTTPAFALYNSGMAYFNLKNYDMALSRYRL
                     ALSQDGVADWQPQIELNIGLAYLRKSDPVRAREHLEKAVALNPSLYEAHFYLGEISLD
                     LRDKAKAKKSFEQVIKLAPQSALGQKSMEYLKTLP"
     gene            10307..11395
                     /locus_tag="PLA58_06275"
     CDS             10307..11395
                     /locus_tag="PLA58_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOR62437.1"
                     /translation="MQQTSQNAFSRRRSSLQRKIPRWGADGILFTDICNIRYLSGFTG
                     SEGLLITGRDETRLLVDGRYTAQARTEVCGIPVCEFADKLEGIEQAAAKMGLQKIGFD
                     PLHMNVSDYDRLIRRLKNVKLIPLGEEARMLRARKDAAEIFLMKKAAEIAAKAVRGLA
                     CALRPGWTEREAALYLETLARQGGADQVAFETIVASGENGAFPHARPTNRKIRRGDFV
                     VVDFGVRCQGYCSDETCTFAIGELTRGQKNAYRAVKRAHDEAIAAIGAGAAAAQIDAL
                     ARRTLGKKYSRYFVHGTGHGVGLEVHEAPRLAPTSGDVLQSGMALTVEPGLYYPGLWG
                     LRIEDTVVVKKNGCEMITSMSKDLITIE"
     gene            11414..11809
                     /gene="gcvH"
                     /locus_tag="PLA58_06280"
     CDS             11414..11809
                     /gene="gcvH"
                     /locus_tag="PLA58_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOR62438.1"
                     /translation="MKVFPEDLLYSREHVWVRVDGDMATLGITDYAQESLGEILSVEF
                     PEIDTYVERDEAFGSIESTKAVVDLIASVSGTIVGVNEDINDDIGIVNSDPHDTGWLV
                     VIEMDDMEQLDDLLDAAGYHDYILRQEAD"
     gene            11819..12637
                     /locus_tag="PLA58_06285"
     CDS             11819..12637
                     /locus_tag="PLA58_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOR62439.1"
                     /translation="MTWRLIPYQSHSAFQNMAIDEAIFRETLKHQKPPTLRFFGWHPA
                     AVSVGYFQDVPKEIHVGRCRSAGVDMVRRMTGGKAVYHQDEITYSLSAGPWERRFPDG
                     ILRTYEIISSCLARGLSELGIHAMLAEKSALARKKERAPCCFSEPAGKELTVDGRKIC
                     GSAQVRSHGGFLQHGSLLMTFDPAATAALIQSPAPPEQLRNRAVGVNELVETPVSAEE
                     LCEALRRGFCDALGVDFCEGELTPEEKAMSDRLVKKYESDAWNRERKKEDGPAV"
     gene            12992..13183
                     /gene="rpsU"
                     /locus_tag="PLA58_06290"
     CDS             12992..13183
                     /gene="rpsU"
                     /locus_tag="PLA58_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOR62440.1"
                     /translation="MEVKVIDNDVEKALKILKNKLSKSGLFKELKVRRAYEKPSVKKK
                     RKTIEARRRLAKVQRRRSN"
     gene            13342..13992
                     /locus_tag="PLA58_06295"
     CDS             13342..13992
                     /locus_tag="PLA58_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOR62441.1"
                     /translation="MEKQYLIDELSREESWRIFRIMAEFVDSIEDLSRVQNGVTVFGS
                     ARVKPGDPYYQMAEKLGRLLVQNGFSVITGGGPGIMEAANKGAAEAGGRSVGMNIKLP
                     FEQKPNPYANLQIDYKYFFIRKVMFVKYAVAYIIMPGGYGTMDEFFEALTLIQTKRVK
                     SFPIIVMGREYWRGLLDWLKNTMLRANMILPFDIEMIQIIDEPEEVVKHIQKYVIV"
     gene            14026..15372
                     /gene="holA"
                     /locus_tag="PLA58_06300"
     CDS             14026..15372
                     /gene="holA"
                     /locus_tag="PLA58_06300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOR62442.1"
                     /translation="MSLEKVLLDLKKGKVAPCYLLYGEEEYLIQESLRDILDLLVPPS
                     DRDFGLFFLDGSEADFDLLRDRLSAPSLLGGRKVVVVKNTEIFQSRENLGDLIQKIRE
                     NLGENQDKAVRSFQMFLKLSGFAWEDLRGAGWQKISDEQWAGAVEDDPGDDRHTWLPR
                     IVEICAGRAMTSGGDADLSDSFEKLLAGGLPAGNCLMLTAEAVDRRKKIFKVIDRTGV
                     VLHFGKIKEAATRETLKSEAQKLLNGCGKSLTSGAWAALGKKTGFQLRPSLNELQKLV
                     VFAGEKTVIDEEDVETVVGKTKEDRIFDLTNALSEKNAKAALLALQSLLEQGEQPLMI
                     LAMISREIRLLLQASILTQSGKLPPVTPGMEFPSFQKNVYPSVSAMAQGMARKEDLLV
                     GKHPFVIFNALRHCRRFSCPVLIGYLDELLEMDRAMKSSATDPQLLLERFLIKACA"
BASE COUNT         3751 a         4010 c         4545 g         3167 t
ORIGIN      
        1 ttttgatagg gaggaattga cgtgttaact ttggaaaaga ggaaagcatt aatcgaagac
       61 taccggcttc acgagaagga taccggttcg cccgaagtac agattgccct gttgagcgcg
      121 aggatcgaat atctgaccga gcatttcaag acgcaccaga aagatcacca ttcccggcgc
      181 ggtctgttga agctggtcgg ccagagaaga agattgctga attatatcaa agaagatgat
      241 gtggaacgct acagaaatat aatcaagcgt ctgggtctca ggaaatagca cgagccattc
      301 aagggcggaa ggcaaaagca tcaccgattg gccatggttt tagttgaacc ccgtgtcaat
      361 cggcttttgc gtttcgccct tgaatttcct gttccggacg acaaacccaa ggaaggcgga
      421 ggaaagtgaa tgagtgacaa tttcagtacg gattttgcgg ggcgtaattt tatactaaag
      481 accaattacg tggccgcgca ggcggacggt tccgcgctgg tgtattacgg ggacaccgtg
      541 gtgctggtga cggcggtatc gttgaaaagc gtcagggagg gcgtggattt tcttccgctg
      601 acggtggatt atcaggaaat gacgtttgcc gcgggcaaaa ttcccggcgg ctttttcaag
      661 agggaggggc gccccaacga gcgcgaaatc ctgacatcgc gcattattga ccgggcgatt
      721 cgccccctgt tccccaaggg gtattattcg gaaacccagc tggtggccac cgtgctgtcc
      781 gtggacaagg aaaacgacgc ggatgtggcg gccatgatcg gcgcatccgc cgcgctcgaa
      841 atatcggaca tccccttcaa ggggccgatt gccggcgtgc gggtcggccg gattggaggc
      901 gagctggtcg cgaacgcgtc gccggaaaaa atgcaggaaa gcgaaatgaa cctttttctg
      961 gtggggcgca aggttacgcc gagccggtcg ggtcgcgcct atgacgtcga actggtaatg
     1021 atggaaggcg aagccaggga agtgccggaa gacattatcg tcgatgccat caaattcggt
     1081 ctggaagccg ttcggccggt gatcgatctg caggaccggg tgcgacaggc ggtcggcaaa
     1141 gccaaacgca gcgtggaaga agccgcgccg gatgaagaac tgatcgcccg ggtgcgcgag
     1201 gaggcgctgc cgggcctcca agaaggctac tcgatgccgc ggaaactgga gcggtacggc
     1261 aagctgggcg acgtccggag cgccgtggtt aaaaaaatcg gcggcgatga cgcggcgctc
     1321 tgcaaaaaag tcgcggcgat cattgaacac ctggaatccc gtattttgcg ggacatgatc
     1381 attcaggaga agaggagaat cgacggacgg tcttcgaccg acatccgtcc catccattcg
     1441 gaagtcggcg tgctcccgcg cgcccacggg tcggctttgt tcaaccgcgg tgaaacgcag
     1501 gcgctggcgg cgctgacgct gggaacctcc tcggatgaac agcgcatgga ctatgtcagc
     1561 ggcgaagaac ttcgttcttt cctgctgcat tacaattttc cgccctacag cgtgggcgaa
     1621 gccaaatcgc ttcggtctcc cggccgcaga gaaatcggcc acggcgcgct ggcgcgcaag
     1681 gcgctgattc ccgtgcttcc gccgccggag cagtttccct acacaatccg gattgtttcc
     1741 gagattctct cttccaacgg gtcgtcctcg atggcgaccg tctgcggcgg tattctgtgc
     1801 ctgatggacg gcggcgtgcc cgtcaaagat attgtcgccg gcattgccat gggactgctc
     1861 aaggaaggtg aagatgtggt catcctctcc gatattcttg gtgatgaaga ccacgcgggc
     1921 gatatggatt tcaaggtttg cggcacggaa aagggcgtta ccgccatgca gatggacatc
     1981 aagatcgacg gtctgacgga ggacatcctg cgcaaagcgc tggcgcaggc ccgggaaggc
     2041 cgcctgcaca tcatcggaaa aatccgtgaa acgctgtccg cgcctcgttc ggatatttct
     2101 ctgtatgcgc cgcgcatcac caccgtgaaa gtgaaggaag accaggtccg cacggtgatc
     2161 ggctccggtg gaaagaatat tcgccagatc atcagcgaaa ccggcgtcac catcgatgtg
     2221 gaagacgacg ggaccgtgac cattgcgtcc gccgacgcgg aagcggcggc ccgcgccgtg
     2281 gcgatggtca aatggctcac cgaagaagcg gaagtcggaa agatttaccg cggcaccgtc
     2341 aaaaagatcg tcgatttcgg cgcgtttgtc gaaattctgc ccggcacgga aggcctgctg
     2401 catatttcac agatcgccaa ggaaagaatc gccaaggtga cggacgttct gcaggagggt
     2461 gacgaagtga tggtcaaggt cctggaggtc gataagtcgg gaaagattcg cctcagccgc
     2521 aaagaagcgc tgggcgcgga agtcaaatag cggtccatcg gttccgaaac gtcattccgc
     2581 gccggttgtc cgccggcgcg gaatgcaatt cttattcttt catggaaacc atcaacgttc
     2641 agattcggaa aattgccggc aacgaggatc ttcccctgcc ggaatacatg acccgtctgg
     2701 cggcgggaat ggatctcttc gcggccgtcg ccgcggagga aacgattttg cccggcgcgc
     2761 gcaaaaaaat accgaccggc atcgtcattt ccctgccgga aggctatgag gcgcagatac
     2821 ggccgcgcag cggcctggcg cttcatcagg gccttacgct tttgaattcc ccgggaacca
     2881 tcgacgcgga ctaccgcggc gaaatcgccc tgattgtcat caatcatgga gatgcgccct
     2941 tcgtggtgaa aaggggcgcg cgtctggcgc agatggtcgt ccagaaaatc tgccgggtgc
     3001 agtgggttga agccctccaa ctggatgaga cgccaagagg cgaaggcggt tttggacata
     3061 cctgagcagc ggccggcatt ttgcatttat cgtcaaacag tggtatgaaa gcggtaaaat
     3121 cgagatgaac aagacgggcg catgaaaaaa ttcaaagtta aaaaaacctt tcaggaaatc
     3181 aacgagaaga tcaaaaaggg gcgggccgtt gttgtcaccg ccgaagaaat gattgacgtg
     3241 gtggaacgcc acggcgatgt cgaggcggcg cgccgcgtgg acgtggtgac caccggaacc
     3301 ttcggcgcca tgtgctcctc cggcgccttt ctgaatttcg ggcacaccgc tccgcgcatc
     3361 cgggcctccc gcgtctggct caacgacgtg ccggcttacg gcggcgttgc ggcggtcgat
     3421 tgctatatcg gcgcgacgga agtcgtcgag ggggatccgc tcaacagcgt ttatcccggc
     3481 gagttcaact acggcggcgg ccacgtgatt gaagatctga cggcaggcaa cgtcgtcaaa
     3541 ttgcgggccg aggggtacgg gacggattgc tatccgtcgc gcaaatttga aaaaaacatc
     3601 accatcaacg atttgcgcga cgcctttctg ttcaatccgc gcaacgccta tcagaactat
     3661 aattgcgccg tgaatctttc cgacacgacg atttacacgt acatgggcat gctgcgtccg
     3721 cgcatggcca acgtttccta ttcgacatcc ggccagctca gccctttgtt caacgatccc
     3781 tattaccgga ccatcggcat cggcacgcgg attttcctgg gcggtgcgca gggatttgtc
     3841 gcctggccgg gaacgcagca caacccccat gcggccaggt ccgccaacgg cgtgccgaag
     3901 gaaggcgcgg gcaccctggc ggtgatcggc aacctgaagg agatgagtcc cgagtggctg
     3961 accggcgcca gcattctggg ttacggcgtt tctttgtttg tcggcatcgg cattcccatt
     4021 cccatcctcg atgaagaaat ggcgcgctat accgcggtga aagatgagga tatttatgcc
     4081 caggtgtatg actacagcat ggattatccc aagggcaatc ccaggcccgt ggcggaagtc
     4141 aactatcggg acctgcgcag cggaaccgtg atcatcaacg ataaaaaagt cattgccgcg
     4201 cctctgtcca gttactacaa ggcccatcag attgccgaac ttctgaaggg ctggatcgaa
     4261 gccggaaaat ttctgctggg agagccgcag cagaatctgc cctccattcc ttttccatga
     4321 tccgacggaa ggataaatag agcaataaat ttaatgctta tgaaatatct ttgcttgact
     4381 ttttatgctg attgtagttt ttttaagcca cagggaaagg cattggaata cggatattga
     4441 accgggagtg ttgatccatg aaaatcggga gatatttagt cgcctttgtt tttttcatga
     4501 tgcttttgat cgccttcggc aatcggggag ttgtcgataa ctatttcatc gccaaaagac
     4561 tgtctcaact gaaagcggaa aacaatgcgc tgattgcaca aaataaagaa ctggcgggaa
     4621 aaattctttt gctgcgaagc gatccggcct atatcgaatc gatcgcgcgc aacgaactgg
     4681 gcatggtcaa acccggagat gtggtttacc ggtggactca gtaatcccaa gccgtcacac
     4741 aacataacgg tgctggaatg gattttaaaa acctgttttc gcaaaccaaa agccttgtcg
     4801 gcctggatat cggctcgacc tccatgaagc ttgccgagat tgtcagcacc tctcagggca
     4861 tgacccttaa ccggtttctt cagattcccg tgccgcccgg ggtcattgtg gaaggcgttt
     4921 tgcatgatcc cgacgcgctg gccggcgtga tcaaggagct gttccggaat tccggctgca
     4981 agggaaaagg cattgtcact tccctgtccg gcaattccgt catcatcaaa aaagtgacgc
     5041 tggcccagat ggaggaagcg gagctgcgcg agttgatcca cgatgaagcc ggcaaatatc
     5101 ttcccttcga caacatggat gacgtgaatt atgacttcca gattctggga gacaatgaat
     5161 tcaatcccaa tcagatggaa gtgatcattg tggccgccaa aaagacggat gtcaacggct
     5221 acctggatgc atttgccgcg gcaggcctgc aggtcatgat catggatgtg gattccttcg
     5281 cgctggaaac catgtacgag gcgaactatg actttgaaga aaatgagatt gtcgtcatcg
     5341 tcaacatcgg cgcgagcctg acgaatatca acgtgatcag gggcggtatg tccgtcttca
     5401 cgagagattt tacgatggcc ggaaacgccg tcaccgaggc gctgcaggaa aggtttcagg
     5461 tgacggcgga ggaagcggag cgcatgaagg tggaagggac ggccggcgca caggaaattc
     5521 cgggcctcga aaacgccatt ctggacgcag ccgagcccat ctgctcggag atcgaaaggt
     5581 ccgtcgatta tttccgctct acgttcggcg gagaagaaat caagcatgtc tttctgtccg
     5641 gcggatcctc gagaattgcc ggattgagcg accttttgtc gcagcggttg aacatcggca
     5701 cgcagttgat caatccgctt gccaaaatcg ggcacaacaa aaagaacatt ggcgaagcag
     5761 aactggacgg catcaaaact atcggcgctg tggccatcgg gttgggttta aggaaaatag
     5821 gtgacaaatg attaaaatca atcttatccc ctaccatgaa aaagaaaaga aagagaatct
     5881 ggcccggcag atcgccgtca tggccggggc ctttcttctt tttatccttt gtctggccgg
     5941 cgtttacgtc tatatgaccg ttcaggtcgg ccgtctggaa acgaagctca cggagtccag
     6001 ggacaagctc aaggcgctgg aggccaaagt gggcgacctg gacaagtata agagccaaat
     6061 tgcggaattc gagctgaagc tcggcgtgat tggcaccctg gaagaaaacc gcgtttaccc
     6121 ggtcaaaatg tttgacgacc tggccatgct ggttccgcaa aaaagcgtct ggctgaccaa
     6181 gatcggacaa agcgatgaaa gcctgactct ggaaggcgtc ggtcgcgaca acatcgcggt
     6241 tgccggtttc atgaaaacga ttgaaaattt ccccgcgatc cagtcggtgg atctggtttc
     6301 gtccaaaaaa gtggagattg ccgaaacgac cctgcagcag ttcatttttt cctgcaagtt
     6361 gaaaaagggg ttttaagcta tggcgatgga attcaactta gacgaactga agaaattatc
     6421 tccgcaggtc aaggccctga tcgtcgtcgt ggtcattttt ctgatcggct acctgtacta
     6481 cttctatttt ttgtccgaca cactgaccaa aagatcgcag atgagcaagg agttggagga
     6541 gcttcagacg cagattcagc agaaagaaaa cattgcgcgc cagatgaaca aggtcaaagc
     6601 gcaggttgcg acgctgaaag aaaattatca ggccgccctg cagaagcttc cttcccggaa
     6661 ggaaattccc ggcctcttct attccgttgc cacggcgggc cgggaagcgg gcgtggaatt
     6721 cgtcctgttt gaaccgctcc cctccgtgcc gcaaaccctg gaaaaggccg aaaaactctc
     6781 gggcaaactg aagccctccg acaagcggca ggaggagcag gaaaagaagg ccgaggctga
     6841 gaaagcggcc ggcaagccgc cgggcgatgc gaaaaaagcg ccgccggcca aagcgccggc
     6901 atcggagcct ttctacgagg aaattccgat gaaggtgacc gtgacgggcg gataccagaa
     6961 cattgttttc ttttttgaca aactggccag gctgccccgg atcgtcaata tttccgatgt
     7021 ttccatggga gaccgaaaag agggcaaggg aaaaaaatat tcgataaccg catcctgcat
     7081 cgtgaaaacc tatatgtttg tggataaaaa agagcaggcc aatgaaaaga aaaaataaga
     7141 aacaagcgat cgtttggttc ggtgtatggc tcttcctggc gacgatgttg ggcgcttgcg
     7201 gggatcagga aaaggcgccg gccgcgcccc ggccgcccgc gaagccgcaa gcggttcaaa
     7261 cggcgccctc cgttcaaccg aaaaccgttc aggccgcccc cgcatcctcc gcaatcccgg
     7321 ccgtccagat gccggcgaac gtttacagct accggccgca aggcaaaccc gatcctttca
     7381 agccgttcgt cgatgcgcaa atcgtcgcgg ctaaaaaagg taaagcggct gcaaccccat
     7441 ccatcttccc gctgcagaag gccggagtgg aaaatttcaa tgtcgtggga attatgggag
     7501 atgacatccg ccgcatggcc attgtcgaag acgcggccaa aaagttttac cctctttttg
     7561 tcggaacgcg gatagggctt cataacggaa aagtaaccga catcctggcg gatcgggtga
     7621 tcgtggatga attggacgga aaaaaaacaa gaagagttgt tttgaaactg cgtcaaaatc
     7681 aataatgagg taagatcatg agaaaatatc tatccagaat cattccggtt gtcatcgtcc
     7741 tctgggtttt tctctataca ccggccggcg tgatgacggg ctcgggcgca caggacaacg
     7801 taggttatct ggaaaacatc ctgctggaaa agcagcccgg aagagagaaa gtcattctgg
     7861 aggtgtctca gcagcctgcg aacattcccg cttctctcca gggggataac agcctcttaa
     7921 tccggctgga aaatttgtat cttccgaacc atctgcggga gacgctcagg ggcaatctgc
     7981 tcaacgtgat tgacgtgcgg gcggcgcaga aaacggagaa cggcaagccc tgggtttatt
     8041 taacgattcg cctgaaggaa aacacgccct attccgttgt gccggaggaa aagaaaatcg
     8101 ttgttgattt caatatttcc ggcttgtcct cgaaattggt tcagccccgc gaaacggcgg
     8161 ccggggaaaa acaagcggcg caagtaggag gtgtgaaaag atataccgac cggatcatct
     8221 ccctcgattt tcaggacgcg gacatcaaga gcgtgctgcg tctgatggcc gaatacggca
     8281 atatcagcat catttccggc gacgacgtca agggcaatat cacgctgacg ctgaaaagcg
     8341 ttccgtggga ccaggcgctg gattccatcc tgaacatcaa gggacttacg aaaaaacaat
     8401 cggacgacgt cattatcgtg atgacgctcg ccaaaaagaa gcaggatgaa gcggaccgtc
     8461 aggcggcctt tgaggctcag caaaaggcgg atgaggagcg aaaagccagg gaacagaagt
     8521 ccgacatcga aaaagggaaa ctgcgccaga tcatgatcga ggcaaagatc gtcgaagcgt
     8581 cggatgattt tatccggaac ctgggcatcc agtggggcgg cggcagcagc cagcgtgtcg
     8641 gcggtactta cggactgggc atttccgggg cgaccaaccc gctcacaacc acgactcgaa
     8701 tgaactatgc gtttccgcct gaaattagtc cggcaatgac gatggccgcg gtcaatatgc
     8761 cgtccgccgt ggcggggcct acgctgggcc tggtattcgg cggcgctcac gggtttatcg
     8821 aagcgcagtt gcaggcgctg gaaaaaacca cccggggcaa gatcatttct tcgccccgcg
     8881 tcgtgacgat ggatgacgtc aaagccatta tcaaacaggg agaagaagtt ccctacatta
     8941 cgccggccac cacctcggct ccccctaccg tcgcttttaa agacgctctg ctgaagctgg
     9001 aggtcaagcc gaaaatcacc gacgacaacc ggatttccat ggacatcaaa gcttccaacg
     9061 actgggcgga ctttaccaag gccaatgctc aaggcgagaa tccgccaatt gttaaaaacg
     9121 aggtggaatc caaagtagtc gtgaaagacg gcgatacggt cgtgatcggc ggcatcgcca
     9181 agatggacga gaacagaata gcggccggcg ttccctggtt ctacaagatt cccgttttgg
     9241 gatggctgtt taaaacggaa aacgtcacta tcaagaagcg tcagttgatg gttttcatta
     9301 cgccgaagat cctgacggaa tccggataca atgaagatga atgcgacccg acgaaaatgg
     9361 acaatctgga ccgctgccgc aaccggaaat aaaccgttgc ttttcccgac attgttccct
     9421 tcgcgtgcgt ccgtccgcgg ggggattttt gtgcaaccct tcctatcaag cttgctccgg
     9481 cgaaatcaac ggagctgatg gaatcatgaa actgaaaacc gttcttgttt tttttctgct
     9541 tgcgggcgcg ctgttctttt ggtcctgcac cagcacgcct tatcaccggg atcaggcgga
     9601 cattcatttg caaaaaggca ttgctttcat tcaggccgga aagtatatcg gcgcgcttgc
     9661 cgagcttctg gaagcggaaa aaaacgcccc cgatgatccg gagatccgtt attatctggg
     9721 tattgcctac cttggccgcg gcatgaaaga ccaggcgatg gagcagtttc aaaaggccgt
     9781 ctctctgaag gaaaattatt ccgaagccca taattacatc ggcgtcatgc ggatggacga
     9841 gggccaatgg cagaaggcca ttgaatcgtt tgaccgggcg ctgaagaatc cggtttatac
     9901 gacgcccgcg tttgcgctgt acaactccgg aatggcctat ttcaatctta agaattatga
     9961 tatggccctg tcgcggtatc gtctggccct gagccaggac ggagtggccg actggcagcc
    10021 gcagatcgaa ctgaacatcg gcctggccta tctgcgaaaa tcggatccgg tccgggcccg
    10081 cgagcatctg gaaaaagcgg tggcgctcaa cccctctctt tatgaggctc atttttacct
    10141 cggggagatt tcactggatc tgcgggacaa ggccaaggcc aaaaaatcct tcgaacaggt
    10201 gatcaaactt gcgccgcaat cggctttggg gcaaaaatcc atggagtatc taaagacttt
    10261 gccgtaaccc gccgggaaac ccgactgatt tacaggaacg cccttcatgc agcaaacctc
    10321 ccagaacgca ttttcgcgaa gacggtcttc tctgcaaaga aaaattcccc gctggggagc
    10381 ggacggaatt cttttcaccg acatctgcaa catccgctat ctttcaggat ttacaggcag
    10441 tgaaggcctg ctgataacag gccgggacga aacccggctg ctggtggatg gacgatacac
    10501 ggcgcaggcc cggacggagg tttgcgggat tcccgtttgc gaatttgccg acaagctgga
    10561 gggcatcgaa caggcagccg caaaaatggg ccttcaaaaa atcggctttg atccgttgca
    10621 catgaacgtg agcgactatg accggctgat ccggcgattg aaaaacgtga agctgattcc
    10681 cctgggcgaa gaggcgagga tgctccgggc ccgcaaggac gccgcggaaa ttttcctgat
    10741 gaaaaaagcg gcggagatcg cggcaaaggc ggtaaggggg ctggcctgcg ccctgcggcc
    10801 gggctggacg gagagagaag cggccctgta tctggaaacg ctggcgcggc aggggggcgc
    10861 cgaccaggtc gcctttgaaa ccattgttgc ctccggagaa aacggggcgt ttccgcacgc
    10921 gcggccgacg aaccggaaga tccggcgcgg ggatttcgtc gtggtcgatt tcggcgtccg
    10981 ctgtcagggg tactgttccg atgaaacctg cacctttgca attggggaat tgacacgggg
    11041 tcaaaaaaat gcttatcgtg ccgtcaagcg ggctcatgat gaagccatcg ccgccatcgg
    11101 ggcgggcgcg gccgcggcgc agattgatgc gctggcgcgc cggacgctgg ggaaaaaata
    11161 cagccgctat tttgttcacg ggacgggcca cggcgtaggg ctggaggttc acgaggcgcc
    11221 gcgtctggcg ccgacgtcgg gcgatgtttt gcaaagcggc atggctctga cggttgaacc
    11281 cggcctctat taccccggtc tgtggggtct tcggattgaa gacacggtgg ttgtgaaaaa
    11341 aaacggttgc gaaatgatta cctcgatgag caaagacctc atcaccattg aatagcattg
    11401 aggttgacct gttatgaagg tatttccaga agacctgctg tacagccgcg aacatgtgtg
    11461 ggtgcgggtt gacggagaca tggcaacgct ggggattacg gattacgccc aggagagtct
    11521 gggggaaatt ctatccgttg aatttcctga aatcgacacg tatgtggagc gcgacgaagc
    11581 cttcggctcc atcgaatcca ccaaggcggt ggtggacctg atcgcttcgg tcagcgggac
    11641 cattgtcggc gtcaacgaag acatcaacga cgacatcggc atcgtcaaca gcgatcccca
    11701 tgacacggga tggctggtgg tgatcgagat ggacgacatg gagcagctcg atgatcttct
    11761 ggacgccgca ggctatcacg attatattct gcggcaggaa gcggactgaa gcccttctat
    11821 gacctggcgg ctcatcccgt atcaatccca tagcgccttt caaaacatgg ccatcgacga
    11881 ggccattttc cgggaaaccc tgaaacatca aaaaccgccg acgcttcgtt ttttcggctg
    11941 gcatccggcg gcggtttccg tcggctattt tcaggacgtt ccaaaagaaa tccatgtcgg
    12001 ccggtgccga tcggccggag tcgatatggt tcgcaggatg accggcggga aagccgttta
    12061 tcaccaggat gaaatcacct attcgctttc ggccggacct tgggagcgcc ggtttcctga
    12121 cggcatcctc cggacgtatg agatcatcag ctcctgcctg gcgcgcggcc tgtctgaact
    12181 gggcatccac gcgatgctgg ctgaaaaaag cgctttggcc cgaaaaaagg aaagagcgcc
    12241 ctgctgtttt tccgagcctg ccggaaagga gcttacggtg gacggccgga aaatctgcgg
    12301 cagcgcacag gtccggtcgc acggggggtt tctgcagcac ggatcgcttc tgatgacgtt
    12361 cgatccggcc gcgaccgccg ccctgattca gtcgcctgcg ccgccggaac agttgagaaa
    12421 ccgagcagtc ggcgtaaatg agctggtcga gactcccgta agcgcggaag agctctgcga
    12481 agctctgcgg agggggtttt gcgacgcgct gggggtcgat ttttgcgagg gggaattgac
    12541 gccggaagaa aaggcgatga gcgaccggct ggttaaaaaa tacgaaagcg acgcctggaa
    12601 ccgggagcga aaaaaagaag acggtccggc cgtttaaagg catcgtattt ttgttgcaat
    12661 ttccatcggg atcgcatata ggaaaacctc caaggcgtgc ggcgaaaaaa gaaacgacgg
    12721 cccagcgccg agggtaaatt tgacgcggat aatcgcggga aagttttttg aagggacttt
    12781 ccgggcaggg agcgtgattt aaaaccgatt ggaaatttca gggtagttga cggcgcaggc
    12841 gttgtgccgg actgcccttt ttttgcgttt aaaaggcaga tgagcgtcat ccttcgacag
    12901 ggatccttcg acaggctcag gatgacacaa acagctcaca ggatgacatt gatttgaaag
    12961 tcaatattca ttattcatga ggaggcaaac attggaagta aaagtcattg acaacgacgt
    13021 ggagaaagcg cttaaaattc tcaagaacaa actctcaaaa agcggattgt tcaaggaact
    13081 gaaggttcgc cgggcctatg aaaagccctc ggtcaagaag aaaagaaaaa ccattgaagc
    13141 gcgccgcaga ctggcaaaag ttcagcgccg ccgcagcaat taacaaaaaa acagcgcgga
    13201 ttattcaatc cgacctcccg tcgcgccgga caggcggacg gcctgttccc cggcggagcc
    13261 ggcgaggggg cgcccgaacg ttggatcgcc cttcgcgaag cgacccgtcg gatccggcgg
    13321 catcatgaag gagggaaccg catggaaaag caatacctca tcgatgaatt gtccagggaa
    13381 gagtcatggc ggatcttccg catcatggcg gaattcgtgg attcgatcga ggacctttcg
    13441 cgggttcaaa acggcgtgac cgttttcggt tcagcccgtg tcaagccggg ggatccgtat
    13501 tatcaaatgg cggaaaaact ggggaggctt ctggtgcaaa acggattttc cgtgatcacc
    13561 ggcggcggcc ccggcatcat ggaagcggcg aacaagggcg cggcggaagc cggcggccgg
    13621 tccgtgggga tgaacatcaa actgcccttc gaacaaaagc ccaatcctta cgccaatctg
    13681 cagatcgatt ataaatattt cttcatccgc aaggtgatgt tcgtcaagta tgccgttgcc
    13741 tatatcatca tgccgggcgg ctacggcacc atggacgagt tttttgaagc cctcacgctg
    13801 atccagacca agcgggtgaa aagctttccc atcatcgtga tgggccgcga atactggcgg
    13861 gggcttctgg actggctgaa aaacactatg ctgcgggcaa acatgatcct gccctttgac
    13921 attgaaatga ttcaaatcat cgatgagccg gaagaagtcg ttaaacacat tcaaaaatac
    13981 gtcattgtat agacgccacc cgtttccccg ccgggagagc cgttgatgtc cctggaaaaa
    14041 gtcctgcttg atctcaaaaa gggcaaggtt gcgccctgtt atcttctgta tggagaggaa
    14101 gaatatctga tccaggaatc gctccgcgat atcctggacc ttctcgtgcc cccgtccgac
    14161 cgggatttcg gccttttttt tctcgacgga agcgaggcgg atttcgatct gctccgggac
    14221 cgtctttcgg cgccgtcgct tttgggcggc cgcaaagtgg tcgtcgtcaa aaacacggaa
    14281 atatttcaat cccgcgaaaa cctgggagac ctgatccaaa aaatccgcga gaacctcggg
    14341 gagaaccagg ataaagccgt tcggtcattc caaatgtttt tgaagctgtc gggatttgcg
    14401 tgggaggacc tgcggggcgc aggatggcag aaaatttccg atgaacaatg ggccggcgcc
    14461 gtggaagacg acccgggaga cgaccggcac acgtggctgc cgcggatcgt tgaaatctgc
    14521 gccggccggg ccatgacgtc cggcggcgac gccgatctct ccgattcgtt tgaaaagctc
    14581 cttgccggcg gactcccggc ggggaattgc ctgatgctga ccgctgaagc ggtggacagg
    14641 agaaaaaaga tcttcaaggt catcgacagg acgggcgtgg tcctgcattt cggcaaaatc
    14701 aaggaagccg ccacccggga aacgctcaaa agcgaagcgc aaaagctgct gaacggctgc
    14761 ggaaagagtc tgacgtccgg ggcctgggcc gcgctgggaa aaaagacggg attccagctt
    14821 cgtccttccc tgaatgagct gcagaagctc gtcgtttttg ccggggaaaa gaccgtgatc
    14881 gacgaggagg acgttgaaac cgttgtcggc aaaaccaaag aagacaggat ttttgatctg
    14941 acgaacgctt tgagcgagaa aaacgcgaag gcggcgcttc tggcgctcca gtccctgctg
    15001 gagcagggcg agcagccttt gatgatcctg gccatgatca gccgggagat tcgtctgctg
    15061 ctgcaggcgt ccatcctgac gcaatccggc aaactgcccc ccgtgacgcc gggcatggaa
    15121 tttccctctt ttcaaaaaaa cgtctatccc tccgtttcgg ccatggcgca aggcatggcc
    15181 cgcaaagaag atttgctggt cggcaagcat ccctttgtga ttttcaacgc gctgcgtcac
    15241 tgtcgacggt tttcctgccc cgtcctgatc ggctatctgg atgagttgct ggaaatggat
    15301 cgcgccatga aatcctcggc cacggacccg cagcttctgc tggagcggtt tctgatcaaa
    15361 gcctgcgctt aaagtttgcg gatcctggcc ctagtctgtt tttcggcgcg ggaaagtctg
    15421 cgggcaggcc cttttttatg tccgacgcgg ccgctcgcgt cctcttcctc ttc
//
LOCUS       DAOSRM010000051        15136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4026_length_15136_cov_103.572973, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000051 DAOSRM010000000
VERSION     DAOSRM010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15136
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4026_length_15136_cov_103.572973"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLA58_06305"
     CDS             <1..198
                     /locus_tag="PLA58_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62443.1"
                     /translation="AYSGDPLAEDPRCFIRERSRPAEKGLGKTIKTCSSEHPDIEAAY
                     RDTRRRPATNPGRMRALPDEQ"
     gene            complement(232..585)
                     /locus_tag="PLA58_06310"
     CDS             complement(232..585)
                     /locus_tag="PLA58_06310"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HOR62444.1"
                     /translation="MPRIRISILVFIIFCLAAGDASAFRCGSGMVSAGDSKARVLIVC
                     GKPTAKEKTGEKNKASTVAKKNSKTKKSGGKEEIWYYNCGSRDYIYALTFEDGVLKKE
                     DTEGKGSGKSDCLGK"
     gene            complement(609..929)
                     /locus_tag="PLA58_06315"
     CDS             complement(609..929)
                     /locus_tag="PLA58_06315"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HOR62445.1"
                     /translation="MKTIRTILLVVILLCASTVPAMAFRCGSNLVTTGDRKVKVQAAC
                     GLPTTKEIICPKKSSSCPTTLEIWYYNCGDNDYIYALTFEDGILTKETTQGSGVGNSY
                     CLGK"
     gene            complement(933..1877)
                     /locus_tag="PLA58_06320"
     CDS             complement(933..1877)
                     /locus_tag="PLA58_06320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR62446.1"
                     /translation="MGYALNTTDDYHLHGLTSLGWELTLCNALFPKNSPCRGALLRDA
                     SFGEHLFRFLDKNIPLKRVKTVLEVGGGMGYLMRDLLTLAPHLQTTMADISPFLLQRQ
                     KESLAGFSVSFLETDFFKMPLPDLRAFDLVILNEVLGDFPTLAAESEPSLSEDPAAAR
                     LSARIADFETTYGLRFAPAENINIGALEAVERLCRAGVPCIYLSEHSCESSFSDPSFP
                     SMNFAPSGNPEKIPLKGHAEFTIKFSALEKVARAFSYEVLRGPYTDLLPVSFNDRVTA
                     ALRAPTPLSGRQEILRQFLYDLYKYEYLLLTLGTAGKG"
     gene            complement(1877..2914)
                     /locus_tag="PLA58_06325"
     CDS             complement(1877..2914)
                     /locus_tag="PLA58_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOR62447.1"
                     /translation="MHFYRKILQHRLPITIAAALAGIGIMMYYASCDTACSYLRGNLL
                     GIDLKYVGVGYMGAIIALAFFRQADLLRMLVAAGIGVEGFLVEFQVRENVFCPFCLAF
                     GALVILMYLINYERRHMTNAWHRKGLYVFGDAKIPFTEGLRLPLLAMMIAGYVFVVLT
                     FSGSATPAYAAETTAAPSYGKGSWELMIFTDYFCPPCQAAEKDLEPELERLLARGDVK
                     ITFVDFPGHKSSSLFAKYFLFAVAANKGYANSLKARSVLFALAGEKKIDQESVLAAAL
                     KSKNIALETVDHKPVFQQWLAMIKRFEIDQTPTCILRFSSAYSRKFIGSDQISRELIP
                     ELQKRFPGLKK"
     gene            3149..5536
                     /locus_tag="PLA58_06330"
     CDS             3149..5536
                     /locus_tag="PLA58_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOR62448.1"
                     /translation="MAPDSEKFIALLQEDHPDLSGWTVVCASERAVDPVRHLIHGRLG
                     GILPRVEGLHAYFNRKISEQKPWHSVPGDEALLTLIQFIAESFPGEKAPARRAAAQLP
                     LITRLAEYQVGRQTIPGSDRFTPAQWEQLEQTLETAEGFRRWLSRKNLFLAELEAGRI
                     NEILPGDREIFIGLPELTPPREKFYRKIKKDRLFIDPPLYGPEPAAAGLLPFDSAKNL
                     ARSFGADVWAARNPDIEFLRLAGLHAAADWVTLEVSRFLGERSETDRLMVCLLDESLT
                     PLLWNRSLRWFGNAVNLAVWLPFSATSAGRRLSAQINRAAQTGRTPDFRGFAAASAAE
                     LSANRQSYIREESEALEAAISLSTLLDDWRGRLGRHLAGAAEKLLEAKKFRVTGSRSA
                     PVQVVGFGQASGETFRRGLIFPVDSGIMPAPPFEGPFLNPVHVPQMRKNVFEYQDLLF
                     RQMLARCGSVVVAAVDDAIRERTPGYYFSLLSEEFEKPATKVAFREKTPEGRGDVSCF
                     IPIDEPLRARLRAFSYSYSSLSKILACPFLFYHQYILRVSPPSFMDDDEKINLQMGTF
                     IHRFLQQISTDRKDRLGDWGPLFDELWESADNEALRSMEGVEIYRLNAKIILQEIDEE
                     ERASGRRILFADNAISCEKEFSGAMAGRYSITGRSDRLALLEGNAEVIDFKYSKKRND
                     FSLSPRETVLERFQKKGILHPAAQLILYRHFIPEAKGARFYFLKETSKNRMMEMPEKE
                     LDAAGELAAAIADHLDGIIQGDALAPGYDSRECDYCRLQSLCGKEGYYRTAGGNR"
     gene            5533..8325
                     /locus_tag="PLA58_06335"
     CDS             5533..8325
                     /locus_tag="PLA58_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOR62449.1"
                     /translation="MMESILLKASAGSGKTSRLTREVFERISAGGKFILALTFTRAAT
                     AEMRSRIMELIASEKGRPLLDRMRLIMEAGRVGYSTIDAFFYRLFAAVNDAPRMADEK
                     AQIDLAREIEKRFRDGIARSGAEKRLIIAARILNTDLDLLWSSLGDEQTVERCLLEME
                     RLDDLESLMKENVLLLRELDSLGRAASALAEGMPKRINEWVIAAMAEYDRFMSSALSG
                     YSDLGQYASLGKNIVWHRSPYSDLNRIFQEYRKTAEKLSLNKALLRELAVAFLCGIYL
                     DAANAVKEEKGVIFFADVRKSLLALDGAASRERPKLMGSYFSLGLDRTEHLLVDEFQD
                     TSTGDMAILLPLVDEILSGPGERGERSFFAVGDWKQMIYAWRGANREALETAIGGYLQ
                     NGVIREQSLPHNYRSTPLLISFFNKLVENLFEGGEKTETQTPPEENRGASEGVSEVSL
                     VQLERQGASEAPWYPAIVEYLQKKKEEYGCSWGDMAVLALTNNSVQKIEAELVAHGIR
                     VSSVKGRQLLSTEEGVSVMLFLTMVLSREGRGAFAAEAAASPLWKERFGNALEIRDEF
                     AAQFPRPFGLAAVSLAIERLRGKVSAVILNIWQDEALAFFGEGGADADEFLARMFRVR
                     WSVRVPEGEDRDSIKVDTVHGTKGLQFAHVFVFWNEEERSDSFYLPSQKCHVRFSAGE
                     TKCLQAGGSNGAREIIEKQNLHLLRMRLERANVFYVAATRAIRTLAVFLPVIKSGEYK
                     TVHQAVLKTFEQFAPEGTKSEQGRFSPLREEGKRTAVWDAPPRRGSEPDPYGEIDPAL
                     VSESIKAGIARGERLHRWLARVLDPDRLPPRGELNPEEYDTAVRFVRRKEVSELLFGP
                     GRLYLEQPISDKNDFGVVDRMIVSEHTVTIMDYKSGSLRGLRRKYEEQKERYTRIMKS
                     LYPSRTVAYHILSIDP"
     gene            8396..10561
                     /locus_tag="PLA58_06340"
     CDS             8396..10561
                     /locus_tag="PLA58_06340"
                     /EC_number="3.1.4.35"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP/cGMP-dependent 3',5'-cyclic-AMP/GMP
                     phosphodiesterase"
                     /protein_id="HOR62450.1"
                     /translation="MAAKKPSQIFLSRGGMVIGSSSGNLQLGIPPETIKDTMQMEGGV
                     PRTFIVPKAMFDVQHGVALAEMEFPVYYNFFFRKGKTRIVCSAKQRTRIETVMAEALF
                     GPEEADPVPEFAVGENTPGFTDLKSEMEYFRKSMRDGGRVELDDLVEFCVPDRRGKAV
                     FGETEVRFDPHGNIRIYDQGKKIAAIGGDAPVMVRKSPPPRVSLDFQPPVFGVTTLGA
                     GHGFDPNADTSGLILWVNHRGMMVDPPVNSTEKLAAVGVSPKRIDKIILTHCHADHDA
                     GTLQKIFQEGKIHLYTTRTIYRSFMKKAAALTGIEAGRLERLVDFYPVCIGKPEIIGG
                     GIFHFHYTLHSIPTIAIQASLAGKSLVYSSDTMNDPQFINQLHAEGVLTKNRRDFLLN
                     FPWDRDLVFHEAGIPPIHTPMSYLCSLPRDVRKRMYLVHVNPDSVPEGCGLRIAPTGL
                     TNTVDLGVSPMPREEAVEMLDVFTGVELFAQLPLEKAREFLLSARVSEHRASDIIFSR
                     GDVGDAFYIVMSGEIDIVLDDRILTTYDTGGYFGEKCLFSDEKRTATARVKTDAKLLS
                     IQKEEMLSLIRGTAAESLLHQIADFQNVELRDAVQDNPILDSLSSTQKTRLHGLIRPF
                     SRALQSGEVIALKGSAAGCAYLIREGGVDVYHRRSRIATLSRGGLFGVRKIFRPKEKK
                     NFLFVARGKTALYCIEHDGLKRYLEDNPGVYVKMYHSPY"
     gene            10670..11818
                     /locus_tag="PLA58_06345"
     CDS             10670..11818
                     /locus_tag="PLA58_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR62451.1"
                     /translation="MQSSDIQRLFRERGRKMVLKEGDVLFCQGEASDAVYFVIGGRLD
                     VYAAQGSSEPVRINTVEAGEMVGELGAITRQQRTATLKAASQVELLSIATPDFRALLS
                     DSLSLVEAMTLTNREHLMDADKARIQFGASNRQMQKRVARLGEEKEQLQELLQLREEL
                     EAMVVHDLRNPLNTVIMALGLVERMKDDVRDPETFTLAMRLAKGAANRMNKLIATLLD
                     IARLEAGKLVLNITEFDLAPLLGEVIEMEQASVTKPVEMVAPTSPGLMVKADREVLWR
                     VVSNLIGNALKFAPASSRIEVTAQRLKDKTLRINVIDAGPGIPEGERERIFEKFTRIK
                     DTERGSYSGMGLGLTFCRMAVEAHGGSIWVEEGPSGIGSCFSVQLPQD"
     gene            complement(11842..12441)
                     /locus_tag="PLA58_06350"
     CDS             complement(11842..12441)
                     /locus_tag="PLA58_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR62452.1"
                     /translation="MKALVKAGVVFLAASLALAGCSAPKSYVVLMENADGTTGKIAVS
                     GAKTETLLSQPRTAAGLSGKSEKPFRVEEQKIQQDFAGALAAQPPLPVSFMLYFKSGS
                     AVLTDESQALIPSIVETARKYPAPDVSVIGHTDTMGDSDYNEQIALQRARSVAEIIRN
                     AGIQVQALTIESHGERNLLVPTPDNTPEPRNRRVEITVR"
     gene            complement(12460..12897)
                     /locus_tag="PLA58_06355"
     CDS             complement(12460..12897)
                     /locus_tag="PLA58_06355"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOR62453.1"
                     /translation="MKRCAAVFMMLLWTLSPAWAADTIGYVKTVSGRAEVIASGKAVA
                     ARVGTPIQMGNQLKTGSDGTLGVTFKDNTVMSFGPDTELTVDEYLYEPGKDRLKFGTS
                     MTKGSLQVFSGVIAKLKPQAVQMKTPAAVIGCRGTRFLVKVEP"
     gene            complement(12926..15067)
                     /locus_tag="PLA58_06360"
     CDS             complement(12926..15067)
                     /locus_tag="PLA58_06360"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOR62454.1"
                     /translation="MRFFPKLSPGLLAPFSAVFLGLAVFLAEPLPLQVLRNAVFDQYQ
                     RWHPRPYQAAPVRIIDIDEESLQRLGQWPWPRTRMARLIEKLRDAGAGVVALDIMFAE
                     PDRTSPERITGDWELPREIRRRLEAMPGHDAILADTLAEGRVVLGFAAEQGLARDHGP
                     ARPFRFVFSGESPLPHLYSFSGAVTSIPPLEKAAAGNGAMNFIPDSDGVVRRVPLIIK
                     IQEQAFPSLAAESLRVVQGAHNYIVRTLPEKGAGIQEIRIGSLTVPATPRGEIWVHYT
                     KSVPDRFIPAWKVLAGEAPREKLEGHILLVGASAQGLMDIRFSPMGTVMPGVEAHAQV
                     LEQILTQTHISRPSWASALEALVILIGGLGLGMTALATPALVSACITAVVLLAAGWGA
                     WLAFVQHRLLLDPVTPGLALLATFILCSIIRHRTSEQRHRWVREAFSRYISPNRVDYL
                     IRNPDQLELGGRRQECSFVFTDLAGFTSLMERMDPADAVSVLNAYLDTMVTIAFRRGG
                     TLDRIVGDSVAIMFSAPVVQPDHRARALACALEMHAFAARYAAESRAKGIALGQTRIG
                     VHTGEVIVGNFGGKTLFDYRALGDAVNTASRLEGANKYLGTLICVSEETLSGCPDAVV
                     RPIASLVLKGKTQTLNVYEPIAAQEANGRKPERDTAYEKAFELLKRRDPLALEAFERL
                     AEERPTDALVRLHRDRLRSGEKGDTIILEEK"
BASE COUNT         3206 a         4201 c         4605 g         3124 t
ORIGIN      
        1 gcctacagcg gagatcccct ggcggaagac ccccgctgct tcatccggga aagaagcagg
       61 cccgcggaaa agggccttgg aaaaacaata aaaacatgca gttcagaaca tcccgacatt
      121 gaagcggctt acagggacac ccgccggcgt ccggcaacaa acccggggcg gatgagggcg
      181 ctgccggatg agcaataggg gacaacggcg gatctttccc gtttcggtcc gctacttccc
      241 caggcagtcg gattttccgc ttccttttcc ttccgtgtct tcttttttca ggacgccgtc
      301 ttcaaaggtc agggcgtaga tgtaatcccg gctgccgcag ttgtagtacc agatctcctc
      361 tttgccgccg cttttctttg ttttactgtt ctttttcgcg accgtggaag ccttgttttt
      421 ttcaccggtt ttttctttgg cggtcggctt tccgcagacg atgagaaccc gcgctttgga
      481 gtcgccggcg gaaaccatgc cggagccgca gcgaaaggcg gaagcgtcgc ctgccgccag
      541 acaaaagatg atgaaaacga ggatgctgat tcgaatccgt ggcataaaca ttccccctgt
      601 gaatgatttt atttgcccag gcagtaggaa tttccgactc cgctgccttg ggtcgtttct
      661 tttgtcagaa tgccgtcttc aaacgtcagc gcgtagatgt aatcgttgtc gccgcaattg
      721 taataccaga tttccagggt cgtggggcaa gaactgcttt ttttgggaca gatgatttcc
      781 ttcgtggtcg gaaggccgca ggccgcctga acttttacct ttctgtcgcc ggtggtcacc
      841 aggttggatc cgcagcgaaa agccattgcc gggacggtgg atgcgcaaag caggatcacg
      901 accagaagaa tggttcgaat ggttttcatg ttctatcctt tccctgccgt tccgagtgtc
      961 agcaaaagat actcgtattt gtaaaggtcg taaagaaact gccggagaat ttcctgcctg
     1021 ccggagagcg gcgtgggagc ccgaagggcg gccgttaccc ggtcgttgaa cgagacgggc
     1081 agaaggtccg tgtaaggacc gcgaagcacc tcgtaggaaa aagcccgcgc cactttttcc
     1141 agcgcggaaa atttgatggt gaattccgca tggcccttga gcgggatttt ttcgggattc
     1201 ccggagggcg caaaattcat ggagggaaaa gacgggtcgc tgaaagacga ttcacagctg
     1261 tgttcgctca aatagatgca gggaacgccg gcccggcaaa gcctttccac ggcctccagc
     1321 gccccgatgt tgatgttttc ggcaggagcg aagcgcaggc cgtaggttgt ttcgaaatcc
     1381 gcgattctgg ccgaaaggcg ggcggctgcc gggtcttctg aaagcgacgg ttcgctctcg
     1441 gccgccagtg tgggaaaatc ccccaggact tcgttgagaa tcaccaaatc aaaagcgcgc
     1501 aggtccggca ggggcatttt gaaaaaatcc gtttcgagaa aggatacgga aaatcccgcg
     1561 agagattctt tttgtctttg cagaagaaag ggagagatgt ccgccatggt cgtctgaaga
     1621 tggggggcca gggtcaaaag atccctcatc agatacccca tcccgcctcc cacttccaga
     1681 acggttttga cgcgcttcag gggaatattt ttatccagga agcgaaaaag gtgctcgccg
     1741 aaagacgcgt cgcgcaaaag cgcgccgcgg cagggactgt tttttgggaa aagcgcgttg
     1801 cacagcgtga gctcccagcc cagggaggtc agcccgtgaa ggtggtagtc atccgtggtg
     1861 tttaatgcgt agcccattac ttcttcaagc cggggaacct tttctgaagt tccggaataa
     1921 gttccctgga aatctggtcc gaaccgataa actttcgcga ataggcgctg gagaagcgca
     1981 ggatgcaggt cggcgtctga tcgatttcaa accgcttgat catggccagc cactgctgga
     2041 agaccggttt gtggtccacc gtctccaggg cgatattttt tgatttcagc gcggcggcca
     2101 gaacgctttc ctgatcgatt tttttctctc cagccagagc aaaaagaacg cttcgggctt
     2161 tcaggctgtt ggcgtagccc ttgttcgcgg cgacggcaaa gagaaagtat tttgcgaaaa
     2221 gcgagctgct cttatggccg ggaaaatcca cgaaggtgat cttcacgtct cctctggcca
     2281 gcagtctttc cagctccggc tccagatctt tttccgcggc ctggcagggc ggacagaagt
     2341 aatccgtgaa gatcatcagc tcccatgagc ccttgccgta ggagggcgcc gcggtcgttt
     2401 cggcggcata ggcgggcgtt gccgatcccg aaaaggtaag gacgacaaaa acatatccgg
     2461 cgatcatcat ggccagcagg gggaggcgca ggccttcggt gaatggaatt ttcgcatcgc
     2521 cgaaaacata gagccctttc cgatgccagg cattggtcat atggcgcctc tcgtaattga
     2581 tcagatacat caggatcaca agggctccga aagccaggca gaaggggcag aagacgtttt
     2641 ccctcacctg aaactcaacc agaaaaccct ccacgccgat tcccgcggcg accagcattc
     2701 tcaacaggtc cgcctgccgg aaaaaggcca gcgcaatgat ggctcccatg tatcccacgc
     2761 cgacatattt caggtcgatg cccagcaggt tcccccgaag ataggaacag gccgtatcac
     2821 acgacgcgta atacatcatg atgccgatgc cggcaagcgc cgcggctatg gtgatgggca
     2881 ggcgatgctg cagaatcttt cgataaaaat gcataggttc aattctttca gcgtttgatc
     2941 tggcgggaat tatagagacg gatgctgagg ttgtcaatag aatgttcagg gatgttcagg
     3001 gattattcag ggatcccgaa tatgcaattt catttgacag gcttcaattg ggtattgaca
     3061 aggaccgccg gcttcttcat agtcgggtca ggcgaaaatg cctccgacaa agacaacgaa
     3121 aagaccttta cattcgaagg aagaaccgat ggcgccggat tcggaaaagt tcattgcgct
     3181 cctgcaggag gatcatcccg atctgtccgg gtggaccgtc gtttgcgcaa gcgagagagc
     3241 cgtggacccg gtgcggcacc tgatccacgg aaggctcggc ggcattcttc cccgggtgga
     3301 ggggcttcat gcttacttca accggaagat cagcgagcaa aagccctggc attcggtgcc
     3361 gggagatgag gcgctgctta ccctgatcca gttcatcgcg gagagctttc ccggtgaaaa
     3421 ggctcccgcg cgccgcgccg cggcccagct gcccctgatt acccggctcg cggaatatca
     3481 ggtcggaagg caaacgattc cgggatccga ccgtttcacc cccgcgcagt gggagcaatt
     3541 ggagcaaacc ctggagacgg cggaggggtt tcgccggtgg ctttcccgaa aaaatttatt
     3601 tctggcggag ctggaagccg gaaggatcaa cgaaattctt cccggcgacc gggagatctt
     3661 tatcggcctg cccgaattga cgccccccag agagaagttt tacagaaaga ttaaaaaaga
     3721 caggctgttc atcgacccgc cgctttacgg gcccgaaccg gccgcggcgg gcttgctgcc
     3781 gtttgattcc gcgaaaaatc tggcccgttc ctttggcgcc gacgtctggg cggcccggaa
     3841 tccggacatc gaatttctgc gcctggccgg acttcatgcc gcggcggatt gggtgacgct
     3901 ggaggtttcc cgctttctgg gcgagcgatc ggaaacggac cggcttatgg tctgtctctt
     3961 ggatgaatcc ctgacgccgc tgctctggaa ccggtcgctt cgttggttcg gcaacgccgt
     4021 caacctcgcc gtctggctgc cgttttccgc aacgtcggcc gggagaaggc tttcggccca
     4081 gatcaaccgg gccgcgcaaa ccggacgaac gcctgatttc agaggatttg cggccgcctc
     4141 agcggcggaa ttgtccgcaa accggcagag ctacattcgc gaggaaagcg aagcgctgga
     4201 agccgccatc tccctttcca cgctgctgga cgactggaga ggaaggctgg ggcgccatct
     4261 tgccggcgcc gccgaaaagc ttctggaagc gaagaaattc cgcgttacgg gcagccggtc
     4321 cgccccggtg caggtggtgg gcttcgggca ggcctcgggg gaaaccttcc gccggggact
     4381 gatctttccc gtggacagcg gcatcatgcc ggcgccgcct tttgaaggcc ctttcctcaa
     4441 tcccgtgcat gttccgcaaa tgcgcaaaaa tgtttttgaa taccaggacc ttcttttccg
     4501 tcagatgttg gcgcgctgcg ggagtgtcgt cgtcgcagcg gtggacgacg ccatccgcga
     4561 aagaacgccc gggtattatt ttagtcttct ttccgaggaa ttcgaaaagc cggcgacgaa
     4621 ggtcgccttc cgtgagaaga cgccggaggg acgcggcgat gtttcctgct ttattccgat
     4681 cgatgagcct ttgagagcca ggctccgggc gttttcctat tcgtattcga gtctttccaa
     4741 aatcctggcg tgtccctttt tattttacca tcagtacatt ctcagggttt ctccgccttc
     4801 cttcatggac gatgatgaaa aaatcaacct gcagatggga acgtttatcc atcggtttct
     4861 gcaacagatc tcgaccgacc ggaaggaccg gctcggcgac tgggggccgc tttttgacga
     4921 actgtgggaa agcgccgaca acgaagcgct ccggagcatg gaaggagtgg aaatctaccg
     4981 actgaacgcg aaaatcatcc tgcaggaaat cgacgaagag gaacgggcgt cgggaaggcg
     5041 gattctgttt gccgacaacg ccatctcctg cgagaaagaa ttcagcggcg ccatggccgg
     5101 acgctattcc atcaccggac gttcggaccg gctggccctg ctggagggga acgccgaggt
     5161 gatcgatttc aaatattcca aaaagagaaa cgacttctcc ctttccccga gggaaaccgt
     5221 tctggagcgc tttcagaaaa aaggcatcct gcatcccgcg gcgcagctca ttctgtaccg
     5281 tcattttatt ccggaagcga aaggcgcccg cttttacttt ctgaaggaaa cctcaaaaaa
     5341 ccggatgatg gagatgccgg aaaaagagct cgacgcagcg ggcgagttgg cggccgcgat
     5401 cgcggaccat ctcgacggga tcatacaggg cgatgcgctg gcgcccgggt atgactcccg
     5461 ggagtgcgac tactgccggc ttcaatcgct gtgcgggaaa gaaggttatt accggacggc
     5521 cggggggaac cgatgatgga aagtattttg ctgaaagcgt cggcaggctc cggaaagaca
     5581 tcgcggctga cccgggaggt gtttgaacga atatccgccg gaggaaaatt cattctggcg
     5641 ctgacgttta cccgcgccgc caccgccgag atgcgctccc gcatcatgga gttgattgcg
     5701 tcagaaaagg gaagacccct gctggaccga atgaggctga tcatggaggc gggccgcgtc
     5761 ggctactcga cgatcgacgc cttcttttac cgcctttttg ccgccgtgaa cgatgcgccg
     5821 aggatggccg acgaaaaggc gcaaatcgac cttgcccggg agattgaaaa gcgctttcgc
     5881 gacggcatcg cgcgaagcgg cgcggaaaaa agactgatca tcgcggcccg gattctgaat
     5941 acggatctgg atttgctctg gagttccctt ggggacgagc agaccgttga gcgctgcctg
     6001 ctggagatgg agcggctgga cgatctggaa agcctgatga aggagaacgt tctcctgctt
     6061 cgtgagctgg attcccttgg ccgggcggca agcgcgctgg cggaaggaat gccgaagcga
     6121 atcaacgagt gggtcatcgc cgccatggcg gagtatgacc ggttcatgtc cagcgccctg
     6181 tccggttatt ccgatctggg gcagtacgca agcctcggca aaaacattgt ctggcatcga
     6241 agcccttact cggacctcaa ccggattttt caggagtacc gaaaaacggc agaaaaactg
     6301 tcgctcaata aggccctgct tcgggagctg gccgtcgcgt ttctttgcgg gatctatctc
     6361 gatgcggcaa atgccgttaa ggaagaaaag ggggtgatct tctttgccga tgtgcgaaaa
     6421 tcacttctcg cgctggacgg cgccgcctcg cgcgaaaggc cgaagctcat gggcagttat
     6481 ttttcgctgg gcctggatcg gacggagcac cttctggtgg atgaatttca ggacacctcg
     6541 acgggcgata tggcgattct gctgccgctg gtcgacgaaa ttttgtccgg tcccggcgaa
     6601 cggggcgaac ggtcgttttt tgccgtaggc gattggaagc agatgatcta cgcctggcgc
     6661 ggcgcgaacc gcgaggccct ggaaaccgcc atcggcggct atctccaaaa cggcgtcatc
     6721 cgtgagcagt cgctgcctca taactaccgg agcacgccgc ttttgatttc atttttcaac
     6781 aagcttgttg aaaatctttt tgagggcggg gagaagacgg agacgcaaac tcccccggag
     6841 gaaaaccggg gcgcgtcgga gggggtttcg gaagtgagtc ttgtccaact ggaaagacag
     6901 ggggcatcgg aagctccctg gtatccggcc attgtcgaat atcttcagaa aaaaaaggag
     6961 gagtacggtt gttcctgggg ggacatggcc gtgcttgccc tgaccaataa cagcgttcag
     7021 aaaatcgaag cggagctcgt cgcgcacggc attcgcgtat cctcggtcaa aggccgccag
     7081 cttctttcaa cggaggaagg ggtctcggtc atgctttttc tgacgatggt tctttcccgg
     7141 gaagggcggg gggcctttgc ggccgaggcg gccgcgtctc ccctgtggaa agaacggttt
     7201 ggaaacgcgc tggagattcg ggacgaattt gccgctcagt ttccccggcc cttcggcctt
     7261 gccgcggtaa gcctcgccat cgaacgtttg cgcgggaagg tttccgcggt catcctcaat
     7321 atctggcagg acgaggcgct ggcgtttttt ggagagggag gcgccgatgc cgatgaattt
     7381 ctcgcccgga tgttcagggt tcgctggagc gtgcgggttc cggaaggtga agaccgggat
     7441 tcgatcaagg tcgatactgt tcacgggacc aaagggcttc agtttgcgca tgtttttgtt
     7501 ttttggaacg aggaagaaag atccgactct ttttaccttc cttcccagaa gtgccatgtc
     7561 cggttttcag cgggcgagac aaagtgcctt caggccggcg gatcgaacgg ggcccgtgaa
     7621 atcattgaga agcagaacct tcacctgctg cgaatgaggc ttgagagggc caatgtgttt
     7681 tacgtcgccg ccacgcgggc gattcggacc ctcgcggttt ttctgccggt gatcaaaagc
     7741 ggcgagtaca agacggttca tcaggcggtt ctcaaaacgt ttgagcagtt tgcgccggag
     7801 ggaaccaaaa gcgagcaggg ccggttttcc cccttgcgcg aagaggggaa aaggaccgcc
     7861 gtgtgggatg cgccgccccg gcgagggagt gaaccggacc cctacggcga aatcgacccg
     7921 gcgctcgtct ccgaatccat caaggccggc atcgcgcggg gcgaacggct ccaccgctgg
     7981 ctcgccaggg tccttgaccc cgaccgcctg ccgcctcgcg gggagctgaa ccccgaagaa
     8041 tacgacacgg ccgttcgttt tgtccgccgc aaagaagttt ccgaactcct gttcgggccg
     8101 gggcggctct acctcgagca gcccatatcg gataaaaatg atttcggcgt tgtggacaga
     8161 atgatcgtgt cggagcacac cgtaaccatc atggattaca aatcaggctc cctgcgcgga
     8221 ctgaggcgga agtacgaaga acagaaggag cgctacacga gaatcatgaa atctctttat
     8281 ccgtcgcgaa ccgtggcgta tcatatcctg tccattgacc cataacgcat ggcgccggga
     8341 cttccgcgac cgggctttgc ggagggtcct taccggcgaa gagggagaaa agatcatggc
     8401 agcaaagaaa ccgtcgcaga tttttctgtc ccggggcgga atggtcattg ggtcgtccag
     8461 cggaaacctc cagttgggca ttccaccgga aaccatcaag gacaccatgc agatggaagg
     8521 gggtgtgccc cgcacattca tcgtgccgaa ggcgatgttt gatgtgcagc acggcgtcgc
     8581 gctggcggag atggaatttc ccgtctatta caatttcttt ttccgcaaag gcaagaccag
     8641 aatcgtctgc agcgccaagc agcgcacgag aatcgaaacc gtcatggctg aggccctgtt
     8701 cggaccggaa gaggcggatc cggtccccga atttgccgtc ggggaaaaca cgccgggctt
     8761 tacggacctg aaatcggaga tggagtattt caggaagtcc atgagagacg ggggccgcgt
     8821 ggaactggac gacctcgtcg aattctgcgt gccggaccgg cgcgggaaag cggtgtttgg
     8881 ggaaacggag gtgcggtttg accctcacgg aaatatccgc atctacgatc agggaaagaa
     8941 gattgccgcc atcggcgggg acgcgcccgt gatggtccgc aaaagccccc cgcccagggt
     9001 cagcctcgat ttccagccgc cggtgttcgg cgttacgacc ctcggcgccg gacacggctt
     9061 tgatcccaac gccgacacgt cgggcctgat cctctgggtc aatcatcgcg ggatgatggt
     9121 ggacccgccc gtgaattcca ccgaaaaact ggccgccgtc ggcgtcagcc cgaagcggat
     9181 cgacaaaatc attttgacgc actgccacgc cgatcacgat gcggggactc tccagaaaat
     9241 ttttcaggag gggaaaattc acctctacac cacccgcacc atttacagga gcttcatgaa
     9301 gaaggccgcg gctctgaccg gcattgaggc aggccggctc gaacggctgg tggattttta
     9361 tcccgtctgc atcggaaagc ccgagatcat cggcggggga atcttccatt ttcactacac
     9421 gctccattcc attccgacca ttgccattca ggcgtctctt gccggaaaaa gtctggttta
     9481 cagttcggat accatgaacg acccgcaatt cattaatcag ttgcacgccg aaggcgtttt
     9541 gacaaaaaac cgcagggatt ttctcctgaa ttttccgtgg gaccgggacc tggtgttcca
     9601 cgaagcggga attccgccca ttcacacgcc gatgagctat ctgtgcagcc tgccccggga
     9661 cgtgcggaaa cggatgtatc tggtccatgt gaacccggat tccgttcccg agggatgcgg
     9721 tctgcgcatc gctcccaccg ggctgaccaa caccgtggat ctgggggtct ctccgatgcc
     9781 ccgcgaggaa gccgtcgaaa tgctggatgt cttcaccggt gtggagttgt ttgcgcaact
     9841 gccgctggag aaggcgaggg aatttctgct ttccgcccgg gtgagcgaac accgcgcctc
     9901 cgacatcatt ttcagcaggg gagacgtggg ggatgccttc tacatcgtca tgagcggcga
     9961 gattgatatt gttctcgacg accgaatcct cacgacgtat gacaccggag ggtatttcgg
    10021 cgaaaaatgc ctgttttccg atgaaaaacg gacggcgacg gccagggtga aaacagacgc
    10081 gaagctcctg tccattcaaa aggaggaaat gctgagtctg atccggggaa ccgccgcgga
    10141 aagtctgctt caccagattg ccgattttca aaatgtggaa ttgcgggacg ccgtgcagga
    10201 caacccaatc ctcgattcgc tttcgtccac gcaaaagacc cggcttcacg ggctgatccg
    10261 gcctttttcc cgcgcgctgc aatccggaga ggtcattgcc ctcaaaggat cggcggccgg
    10321 ttgcgcctat ctgatccggg agggcggggt cgatgtttat catcgccggt cccgaatcgc
    10381 gacgctttcc cggggcggcc ttttcggcgt gagaaaaatc ttccggccaa aagagaaaaa
    10441 gaactttttg tttgtcgcaa ggggtaagac ggctctgtat tgcattgaac atgacgggct
    10501 gaagcgttat ctggaagaca atccgggcgt gtatgtcaaa atgtaccaca gcccctacta
    10561 aaagcggcgg gcggcatcgc aaaagcccgt cggcgagggg agcgcaaccg gtgattcggc
    10621 ggcgcacagc gatatctttt atttcagaaa ttcgaaggga gagggcatca tgcaatcatc
    10681 ggacattcag cgtttgttcc gggagcgcgg ccggaaaatg gttctgaaag agggagacgt
    10741 gttgttttgc cagggagagg ccagcgacgc ggtgtacttc gtcatcggcg ggcgtctgga
    10801 tgtttacgcg gcgcagggct cgtcggagcc tgtcaggatc aacacggtcg aagccggtga
    10861 aatggtgggc gaactggggg ccatcacaag gcagcagcgg accgccacgc tgaaggccgc
    10921 ctcgcaagtg gagcttttat ccatcgcgac gcccgatttc cgggcgctgc tgtccgacag
    10981 cctgtctttg gtagaggcca tgaccctgac caaccgggag catttgatgg acgcggacaa
    11041 ggcacggatt caattcggcg cctccaaccg gcagatgcaa aagcgcgtcg ccagactggg
    11101 cgaggagaag gagcaactgc aggagctcct gcagttgcgc gaagagttgg aagccatggt
    11161 ggtgcatgat ttgcgcaatc ccctcaacac cgtcatcatg gctctcggcc tcgtggagcg
    11221 catgaaggac gacgtcaggg accccgaaac ctttacgctg gccatgcgac tggccaaagg
    11281 ggccgcgaac cgcatgaaca agctgattgc cacgctgctg gacattgccc ggctggaggc
    11341 cgggaagctg gtgctgaaca ttacggaatt tgatctggcc ccgctgttgg gagaagtgat
    11401 cgaaatggaa caagcgtccg tgacgaagcc tgtcgagatg gttgcgccga catccccggg
    11461 cttgatggtg aaagccgacc gcgaagtgct ctggcgcgtg gtgtccaatc tgatcggcaa
    11521 tgcccttaaa tttgcgcctg cctccagcag aatcgaggtc acggcgcaac gcctgaaaga
    11581 caagactctg cgtatcaacg tgatagatgc cgggccgggc attccggaag gcgagcgcga
    11641 aagaattttt gaaaagttca cgcggatcaa agatacggag cgcgggtctt acagcggcat
    11701 gggtctgggc ctgaccttct gccgcatggc tgtggaagcc cacggcggtt ccatatgggt
    11761 tgaagagggt ccttcgggca tcgggagttg cttcagcgtg caactccccc aggattgatc
    11821 gaccggcttc ggtttgccgg attaccggac ggtgatttcc accctgcggt ttctcggttc
    11881 cggcgtgttg tccggtgtgg ggacgagcag attcctttcg ccgtgagatt cgatcgtcag
    11941 agcctgtact tgaatcccgg cgttgcggat gatttccgcc acggaccgcg cccgctgcaa
    12001 agcgatctgt tcattgtaat cgctgtctcc catcgtgtcc gtatggccga tgacggaaac
    12061 gtcaggagcc gggtatttcc ttgccgtctc cacgatggac gggatcaggg cctgcgactc
    12121 gtctgtcagc accgcgctgc cggatttgaa gtaaagcatg aaactgaccg gcagcggcgg
    12181 ttgagcggcc agcgccccgg cgaagtcctg ctggattttc tgttcctcta cccgaaaggg
    12241 cttttccgat ttgccggaaa gccccgcggc ggttcggggc tggctcagga gggtttcggt
    12301 ctttgcgccg ctgacggcta tttttcccgt cgtaccatcc gcgttttcca tcagaacaac
    12361 ataagatttg ggcgctgaac agccggccag cgccagcgac gcggcaagaa acaccacgcc
    12421 tgccttgaca agagccttca tgcgatgatc ctcctgattt cagggttcca ctttgaccag
    12481 aaagcgggtt ccgcggcatc cgatcacggc ggccggcgtc ttcatctgga cggcctgcgg
    12541 cttgagtttg gcgatcacgc ctgaaaagac ctgcagggag cctttcgtca tgctggtgcc
    12601 gaatttcagc cggtctttcc ccggttcata caggtattcg tccaccgtca gttccgtatc
    12661 cggcccgaac gacatcacgg tgttgtcctt gaaggtgacg cccagtgttc cgtcggaccc
    12721 ggttttcaat tggttcccca tctgaatcgg cgtgccgaca cgggcggcga cggcctttcc
    12781 ggaggcgatc acttccgctc tgcccgacac ggttttgacg tagccgatgg tgtctgcggc
    12841 ccaggcgggc gacagagtcc aaagcaacat catgaatacc gctgcgcatc gtttcatggc
    12901 ttttcctcct tgacaaagga ttcgtttatt tttcttccag aataatcgta tcaccctttt
    12961 cccctgagcg caaccggtcc cggtgaagac ggaccagcgc gtcagtcggc ctttcctcgg
    13021 ccagccgctc gaacgcttcg agggcgagcg gatcccgcct tttgaggagt tcaaaagcct
    13081 tctcgtaggc cgtgtcccgt tccggcttcc ggccgtttgc ttcctgcgcc gcgatgggct
    13141 catagacgtt gagggtctgt gttttgcctt tcagcaccag ggaagctatg ggacggacga
    13201 cggcatcggg gcagccggaa agtgtctctt ccgacacaca gatcagcgtg cccaggtatt
    13261 tgtttgcccc ttccagacgg gaggccgtat tgactgcgtc tcccagcgcc cggtagtcga
    13321 acagggtttt gccgccgaag tttccaacaa tgacctcgcc cgtgtggacg ccgatgcggg
    13381 tctgtccgag ggcgatgcct tttgcccggc tctccgccgc atagcgcgcg gcgaacgcat
    13441 gcatctcgag cgcgcacgcc agggcccggg cgcggtggtc cggctgcacg accggcgccg
    13501 agaacatgat cgcgacggaa tctccgacga tccggtccag cgttccgcct cggcgaaagg
    13561 cgatggtcac catcgtgtcc agataagcat tgaggacgga aaccgcgtct gccggatcca
    13621 ttctttccat cagacttgta aacccggcca gatcggtaaa aacaaaactg cattcctgac
    13681 gccgtccccc cagttcgagc tggtcggggt tgcggatcag ataatcgacg cggttggggg
    13741 agatgtagcg ggaaaaagcc tcccgcaccc accggtggcg ttgttcgctg gttcggtggc
    13801 ggatgatgct gcacagaatg aatgtggcca gaagcgccag acccggtgtg acggggtcca
    13861 gaagcaggcg gtgttgaaca aaagcaagcc aggcgcccca tccggcggca aggagaacaa
    13921 cggcggtgat gcaggccgaa acaagcgcgg gggtggccag agcggtcatt ccaagtccga
    13981 gtcctccgat caaaatgacc agggcttcaa gagcggaagc ccaggaaggc cgtgaaatat
    14041 gcgtttgtgt caaaatctgc tccaggacct gggcgtgcgc ctccacgccg ggcatcactg
    14101 ttcccatcgg actgaaccgg atgtccatga gcccctgcgc cgaggcgccg accagcagaa
    14161 tatggccttc gagcttttcc cgcggcgctt ctcccgccag caccttccat gccggaatga
    14221 accgatccgg gacggatttt gtatagtgca cccagatttc tccccggggc gtggcgggaa
    14281 ccgtaaggga gccgatccgg atttcctgaa tgccggcgcc cttttccggc agtgtcctga
    14341 cgatataatt gtgcgcgccc tgaaccaccc tcagggattc cgcggcaagg gacgggaagg
    14401 cctgctcctg aattttaatg atcaggggca cccggcgtac aacgccgtcc gaatccggga
    14461 taaagttcat ggcgccgttt cccgcagccg ctttttccag cggggggatc gacgttaccg
    14521 cgccggaaaa ggaatacaaa tggggcagcg gggattctcc ggaaaaaaca aagcgaaacg
    14581 gccttgccgg gccatgatcc ctggcaaggc cttgttcggc ggcaaagcct agaacgacgc
    14641 gcccttcggc cagtgtatcg gccaggattg catcatggcc gggcatggcc tccaggcggc
    14701 ggcggatttc acggggcaat tcccagtcgc cggtgatccg ctccggagac gtccggtccg
    14761 gttcggcaaa catgatgtcc agggccacga cgcctgcgcc tgcgtcccgc agcttttcaa
    14821 tcagccgggc catccgcgtg cggggccagg gccactgacc gagtcgctga aggctttcct
    14881 cgtcgatgtc gatgatgcga acgggggcgg cttgataggg acggggatgc cagcgctggt
    14941 actgatcgaa aacagcgttg cgcagcacct gcagcggcag cggttccgcg aggaaaacgg
    15001 ccagacccaa aaaaacggct gaaaagggcg ccagcaggcc gggggataat ttgggaaaaa
    15061 acctcatgat ttcaccgtcc ggtttgatca aaaggatacg acaggcggca ggatatgtca
    15121 attggcgcat catccg
//
LOCUS       DAOSRM010000052        15031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4060_length_15031_cov_82.895767, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000052 DAOSRM010000000
VERSION     DAOSRM010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 15031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15031
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4060_length_15031_cov_82.895767"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..746
                     /locus_tag="PLA58_06365"
     CDS             162..746
                     /locus_tag="PLA58_06365"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOR62455.1"
                     /translation="MISRIDHVSIAVKDQEKAKAEHFFRDILGAVAGAGMADPVSRFF
                     WQIYSLGDLSRLEIISPTASGSFLDGFLSRREAGVHHITLQTPDIRKAMTHLEAHGIP
                     FFGFNEYPGGVWKEIFIHPRHGFGVLIQIAEFEADDWLGEQVKFPAGTKWQIEKKENG
                     AALIFAHPGGGKVSLELGREDLARLAEKIRELLG"
     gene            complement(798..1886)
                     /locus_tag="PLA58_06370"
     CDS             complement(798..1886)
                     /locus_tag="PLA58_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOR62456.1"
                     /translation="MAKSGNRKTLTALSLQGGGALGAYEYGVLKALYEKRGKGFRPRV
                     VAGVSIGAINAALLIGAKGDPLEALDRLWRERLCVSWPSLFDSSWTGFVPWSMIGLAE
                     RNLSFLGNAGMYCLKPEFFLMPALAWLHNSSLYDTSFLRETLADFVDPEKLNHPEKTR
                     LIVTAVNVKTGLLSRFDNSKMELTFDHIIASGSFPVTFPMTQIGEDFFWDGGVFMNMP
                     VGEAVNALEQIEADNPDVEREVIAIELHRMQADLPATLPEAAERFYNLIFSGKFALDR
                     KLYDRYGAFVDLIGEIDRTIPADSPIRNHRGYKMMTRHRKIDRAMVIGEEGRGATGSG
                     GDFSRNALNRRIEDGYSDAMAFFKKNRL"
     gene            complement(1913..3340)
                     /locus_tag="PLA58_06375"
     CDS             complement(1913..3340)
                     /locus_tag="PLA58_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOR62457.1"
                     /translation="MIRKLFLFSLILLIAGCAVGPDYRRPEVSVPGKWRFEDKEARTL
                     VNLKWWEQLRDPVLNALMETALQENKDVLIAAARIEEYSGRYIAARGELFPQAQASGS
                     ASRQQATEQGYAPWPAGVSNPYPNYQATLNASWEIDLWGKLRRSTEAARADLLSRENA
                     HRAVLLSLTAAVAGAYIDLRDMDRQLEISLDTLKIREESLEIYKMRFDAGVVSELELS
                     QMRSEYENTRATIPRIEKSIAQQENALNLLLGRNPGPVARGLTIDQLNLPPVPAGLPS
                     DLLEQRPDIRQAEQDLISANARIGVARAAYFPSVSLTGMYGVASADLSNLFSGPAQIW
                     NLGAGVTVPIFTAGRIRGQVKASEAIEQQALHNYVKSIQTAFREVEDSLVDQNRTRIQ
                     LDALFNQLTALRNYRDLARLRYENGYSSNLEVLDAERNLFSIELTYIQTRGSLFHALV
                     NLYKAMGGGWSVDGLPQKENPKAEK"
     gene            complement(3333..6515)
                     /locus_tag="PLA58_06380"
     CDS             complement(3333..6515)
                     /locus_tag="PLA58_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HOR62458.1"
                     /translation="MISRFFIDRPVFASVISIVIVIAGLVAMNNLPIAQYPDITPPVV
                     TVSATYPGASADVISQNIAAPIELQVNGADNMIYMTSTSSSAGMMSLNVYFNIGTNPS
                     LAQVDVQNRVNQALPQLPQAVTQQGVIVSKKSSSFLMVIGIYSPNDRYDENYVANYAN
                     LYILDAIKRIPGASEASIMGSPDYAMRIWLKPDRLIQLGITASDVANAVRNQNQQFAV
                     GKIGQSPTRGRVEQTFSVTTRGRLVDPAEFENIILRTDAQGAAIVRLKDVGRAELGKQ
                     DYSVRCRMNGKDTTIIAVYQQAGANALEVSEQVEKTLKTLKTGFPDGIDYKIAMDTTK
                     FVRASIKEVVKTFFEACLLVIIVVFLFLQSLRATIIPILAVPVSIIGTFTGMLLFGFS
                     INMLTLFGMVLAIGIVVDDAIVVIENVERNMRELNLPPKEAAKKAMEEVTGPVAAIVL
                     VLCAVFIPVAFLGGITGQLYKQFAITIAISVIFSGIVALTLSPAMAAILLKPQHGQKN
                     WFFRKFEEKFASLTEGYSRVVQLVIKRAAVALCIFVIMIACVIGLFRHIPSSFVPSED
                     QGYLLGVSILPDAASLDRTQDLNQKARGIYAKQPAIENVVEVDGYSLLDSQVKTNAGL
                     QFISLLDYEKRKEKNLQAPAVIDQTSKMFLGLREGFVLPLNPPSIPGLGTTAGFEFWI
                     QNRGTGDMNRMESITKDFVARAKKQPELQGVATLFNVHSQQLLVDVDREKSENLGVPV
                     EDVYNTLQILFGSAYVSQFTKFSRLWQVILQAEPEYRSRPGDMDQIYVRSRSGNMVPL
                     KALVKKKDVSGPDLVTRFNNFPAAKVIGNPASGYSSGDAIAAMERVADEVLPEDFGYA
                     WSGEAFEEKQAGGTSAVVFVFGLIMVFLILAAQYEKWSLPFGVLLAVPFAICGALLAI
                     WFRGQENDIYFQIGLITLIALAAKNAILIIEFAVLKREQGFNAYDAAVQASKMRLRPI
                     VMTSLAFILGCVPLAVASGASANSRHSIGTGVIGGMLGATLIAVFFIPMFYFLLEKLT
                     QRFGHSGKQKGAPQTEPLFPQPQAQEEKHD"
     gene            complement(6527..7753)
                     /locus_tag="PLA58_06385"
     CDS             complement(6527..7753)
                     /locus_tag="PLA58_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOR62459.1"
                     /translation="MNGTGRPLRLFVSPILFLGLTILLSAALSGCGDKSEKEHARPAI
                     PVTVMTVTPRDVPIDIEFVGTTESSHQVEIRARVEGFLEKKTYEEGGRVRSGQTLFQI
                     DRRPFEASLQQARGALAQQEAKLVNAEATLKRVRPLAEKNAVSQKDLDDAVSAEKSAR
                     AAVFSAQGNVQDAELKLSYTTITSPIDGLAGRAKQQEGSYISVGQSSLLTYVAKIDPI
                     WVDFSISENENLKLMDNLAKGLFKMPKGYDFEVEVHMADGSVFPHRGRLNFADPSYNS
                     ETGTYEVRAEIPNPINSPKVIRPGQFVRVHLLGGIRPDGILIPRTAVSQGAQGFFVWI
                     AGKDSRAEFRAVAVGDWQGNDIFINSGLQAGDRVILDNLLKMSPGAPVAVSPAPAAGS
                     AVPAGAKKSPAPPAKK"
     gene            complement(8138..8473)
                     /locus_tag="PLA58_06390"
     CDS             complement(8138..8473)
                     /locus_tag="PLA58_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR62460.1"
                     /translation="MNVDANLDAKRLIGIRIGNIRKKIGLTQEELAAKMNIGPKYLSS
                     IERGKENPTLNTLINLSHSLGVDLGEIFNAVQIEDVAKRKSLIIALINEAEGEQLKQA
                     YKILTSILR"
     gene            8895..10016
                     /locus_tag="PLA58_06395"
     CDS             8895..10016
                     /locus_tag="PLA58_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62461.1"
                     /translation="MKTKHVLRTGFLLFIVIFFLNVAHGATVQQATGAGTSKEQALYA
                     AMRQAVEKQVGVHIQSNTIVIDAAVLEDKIFSHSQGYVTNYKIIREDKTSDGYSVTIQ
                     AYIDQKLVKEDVNALAILRKGIGNPRILVVFKPRAEGSSAIKGKNFVEKILDGIEDAF
                     SERQFRLVDRNAAAAFASQLEQTHDIDTENNKAAAYGLKYNAEYTLFYSTDGEITERG
                     DSFAANLHVDARLIDNTLSQLITNKTVDASGEGRTAENALEKAGRAGGKNIVAPIISV
                     LQRHWMDMQQNGSLYIVVLDGVDDADEIAQFTEMFEKYPRVNSAREVESGGGKTTFET
                     AYRGKRDELDRDVLRTAKYLGWKLTKVRAEGARTTWKKQ"
     gene            10032..10442
                     /locus_tag="PLA58_06400"
     CDS             10032..10442
                     /locus_tag="PLA58_06400"
                     /inference="COORDINATES: protein motif:HMM:NF018889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HOR62462.1"
                     /translation="MNNFSRLPLGVVIVGLVFLMTSCSTAQTVPERTMTLSEFFAVPS
                     SKLSSTADSIVVRGARTAFIKGVMRVDFKIYNDRGRRNIVNYRMRWLDKDGLMAAPYL
                     DWTTIALEGQQEIVITAVCPNNKAVDYILELRTN"
     gene            10487..12016
                     /locus_tag="PLA58_06405"
     CDS             10487..12016
                     /locus_tag="PLA58_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62463.1"
                     /translation="MKSKLILPAILCLCFLTPLIVFAEMHEFQGEGTAPINPDNISST
                     RMAATKNAKRSAINAAIRKFIGPTAANDPKVKEAIENILTQIDDSKIISRKADKDGDN
                     YVLKITLKVDDLEFRRLLQTEGIAPSTDRTSKILVLMDEYHTTPTDMQKPLKEVVEYS
                     HDKTATAEASLSAASSASSETKAAARESSASSASYAAKARYGAHASGHGYSVGGHASG
                     QVAASGAQRSQSSADYSDKSSESASLEAKSFDQQKDVVNFKKLVEYQPRNVGPAKSNA
                     TYNALLNMAQQYDLNVMNSAVFRSKYFPNKSVTLADLADGSELGKHVQQAGKEGAEYF
                     MMGNAIMRDLGNNQCDGDVTISAYSVDDSTNLTAATHNESARGTSPDDCRVTLAKKLA
                     HFVGKTIGSSIKDYNRQREVSGKEYRVLLLSSADGLGGRMITSFSNNLLKIKGLNSKL
                     NEREKSSSKYDVVLTYKGDVPFSNLITEVLDSMPAMNQADFDVAGTTITICLEGKNRC
                     K"
     gene            12098..14281
                     /locus_tag="PLA58_06410"
     CDS             12098..14281
                     /locus_tag="PLA58_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62464.1"
                     /translation="MKRLIFIVILLSICLLPTVPEAAPKVYPVKRVFGFRQSTLANAP
                     AFQKWVASRSAAGEMREARSILSKEFEAQFKKTFGSLSADNITDVNKHEVLVASLHLL
                     RASEYTVPKIGFSEVSMPITLSIIITNPATGEALYSFTKTAYATTRVVKTNGGNKEVL
                     SQELPSQNAGSQEQLLAAERNAQEAEEDREMFVNARPSDQEQAQMIANSGANYQALVA
                     SLLEEAKAGYNPSNIEIKIADIWKGFYILDKGSKFGIVKGDNITDASGNELTVVHAAE
                     NYAVAQSLLGKVDRDRKFFKYSTGSAAGQFNKPRVLTMHEGWRDPILRGISYIFDSQV
                     PKESAFTLLPVNEHFTRLLHCVAQDTYAGKYETTNQRAMPNYLIKFSATKPRFYTISE
                     EGKFSQHIYEQSVVGELLDRQGRVLFSAVGTDRIEDENVAGMVFAVKDRLEILLKNAT
                     VQLAKQFSNAIKFSRFELPVTGIDGKVIEIKDKSMDLRIGKDVVIYRNIGSIKGIATD
                     VVVPLWQAAVVDVQNDTVKLNLIMKLTDEEISVSTDDLVIMDAVSAAGAANQSNTSIK
                     YCTHERSQRGPLAIDDFEVVSRAYGYLSPYTLYNQDEDFTKKIRDAAKFGGFRDTLEL
                     NKTDTAGRCLLPVYRASRGEKGTDQTIINCDENGFCTDTIEIAVGYRLYINNERKGGS
                     ASKTKVTVKDCKRDVCAPVMQSELSKINRDLLKANIVQIIFKQGE"
     gene            14285..14956
                     /locus_tag="PLA58_06415"
     CDS             14285..14956
                     /locus_tag="PLA58_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62465.1"
                     /translation="MRLNRVIIVGILFAVLSLGTAYAGLFDAVTSIAGGGSSGGDDLD
                     TFLVRAKTAEELINNSTKSMFKAIASDKEQAQLEAKQKKLNETTDPKEKEALQRELTE
                     SQLAVIKKEAGNEELQKEASKWSDKKKEHVANAFFNYALGALQAGLIVKDGKALVTNP
                     ANAVKAATKLGEVKDAIASLGGIASGSVTMISSLKTFMSAANISATIPKTATDKPKPQ
                     ADVDD"
BASE COUNT         3638 a         3712 c         4202 g         3479 t
ORIGIN      
        1 cggcggtctc gtaaaaagta atccaagtcg tccccccggc gaaggccggg gcctatatag
       61 ctcattgtaa ttactggata ccggctttcg ccggtaagac gagaagagtg agaaattgac
      121 tttttacgag ttcatcaaaa tccatgaagg agatgaaaaa aatgatttca cgcattgacc
      181 atgtttccat cgcggtaaaa gatcaggaaa aggcgaaggc cgagcatttt tttcgcgaca
      241 ttctgggagc ggttgcgggg gccggcatgg ccgaccccgt ttccaggttt ttctggcaga
      301 tttattccct gggcgatctg tcccgtctgg agatcatttc tcccacggcg tccggaagtt
      361 ttctggacgg ctttctgagc cggcgagagg cgggcgtcca ccatatcacg ctgcagacgc
      421 cggatatccg caaagcgatg actcatctgg aagcccacgg gattcccttt ttcggattca
      481 acgaatatcc cggaggcgtc tggaaggaaa ttttcattca tccccggcac ggcttcggcg
      541 ttctgattca aattgccgaa ttcgaggccg acgactggct tggggagcag gtcaagtttc
      601 cggcgggaac gaaatggcag attgaaaaaa aggaaaacgg agcggcgctg attttcgctc
      661 atccgggcgg cggcaaggtt tccctggaac tggggcggga ggatctggcg cggctggcgg
      721 aaaagatccg ggaacttctc ggttgaaacc cggagccatt acgcaacggg cgggcgaggc
      781 atctctgccg cggatcgtta gaggcggttt tttttaaaaa aagccatggc gtcgctgtag
      841 ccgtcttcaa tgcgccggtt cagggcgttt cgtgaaaaat cgccgccgga gccggtcgcg
      901 cccctgcctt cttccccgat gaccatggcg cggtcaattt tgcggtggcg ggtcatcatt
      961 ttgtatcctc tgtggtttcg gatggggctg tcggcgggga tcgtccggtc gatttctccg
     1021 atcaggtcca cgaaggcgcc gtaccggtca tacagtttcc ggtccagggc gaattttccc
     1081 gaaaagatca gattgtagaa gcgctcggcg gcctccggaa gggttgccgg caggtcggcc
     1141 tgcatgcggt ggagctcgat ggcaatcact tcccgttcga cgtcgggatt gtcggcttca
     1201 atctgttcca gagcatttac cgcctccccg accggcatat tcatgaagac gcctccgtcc
     1261 cagaaaaaat cttctccgat ctgcgtcatg ggaaacgtga cgggaaagct tccgctcgcg
     1321 atgatgtggt cgaaggtcag ctccattttg ctgttgtcaa agcgcgacag cagaccggtt
     1381 ttgacgttca cggcggtcac gatcaggcgc gttttttccg ggtgattgag cttttccgga
     1441 tcgacgaaat ccgcgagggt ttcccgcaga aacgatgtgt cgtagaggct gctgttgtgc
     1501 agccaggcga gcgcgggcat caggaaaaat tccggtttca ggcaatacat gccggcattg
     1561 cccaggaagg aaaggttccg ttcggccaga ccgatcatgg accagggaac aaaacccgtc
     1621 caggaggaat cgaaaagcga tggccaggac acacataacc gttcccgcca gagccggtcg
     1681 agggcttcca aaggatcgcc tttcgcgccg atcagcaacg cggcattgat ggcgccgatg
     1741 gatacgcccg ccacgaccct cggacggaag cctttgccgc gcttttcgta aagcgctttg
     1801 agcacgccgt attcatacgc tcccaacgct ccgccgccct gcaaggaaag ggccgtcagc
     1861 gttttgcggt ttcctgattt tgccatgagc cactgctccc gtataaaaaa tgttatttct
     1921 ctgcctttgg gttttccttt tgaggaagtc cgtccacgga ccatccgccg cccatggcct
     1981 tgtagagatt gacgagagcg tgaaacagac tgccgcgggt ctggatgtag gtaagctcga
     2041 tgctgaaaag attgcgctcc gcgtcgagaa cttccaggtt gctgctgtat ccgttttcat
     2101 agcgcagacg cgcgagatcc cggtaattgc gcagggcggt caattgattg aacagggcgt
     2161 ccagttgaat tctggtccgg ttctgatcca ccagggaatc ttccacttcc cgaaacgccg
     2221 tctgaatgga cttgacgtaa ttgtgcagcg cctgctgctc aatggcctcg gacgccttga
     2281 cctgtccccg gatgcgtccc gccgtaaaaa tgggaacggt gacgcccgcg ccgaggttcc
     2341 agatctgggc gggccccgaa aacagattgg agaggtccgc gctggcgacg ccgtacattc
     2401 cggtgagcga cacggaaggg aagtacgccg ctctggcgac gccgatccgc gcgttggcgg
     2461 aaatcaggtc ctgctccgcc tgccggatat ccgggcgctg ctccagaaga tccgacggca
     2521 gacccgccgg aacgggaggc agattcaact ggtcgatggt cagcccgcgg gcaacggggc
     2581 ccgggttgcg ccccaaaaga aggttcaggg cgttttcctg ctgggcgatg ctcttctcga
     2641 tccggggaat cgtggcgcgc gtgttttcgt attcggagcg catctggctc agctccagct
     2701 ccgagacgac gccggcgtca aaacgcatct tgtagatttc cagggattcc tcccggatct
     2761 tgagcgtgtc cagagaaatc tccagttgcc ggtccatgtc ccgcaaatcg atgtaggcgc
     2821 cggcaaccgc cgccgtcagg gacagcagaa cggcgcggtg ggcgttctcg cgggaaagga
     2881 ggtccgcccg ggccgcttcg gtggagcggc gcagctttcc ccagagatcg atttcccagc
     2941 tcgcattgag ggtcgcctgg taattgggat aagggttgga aacgccggcg ggccaggggg
     3001 cgtacccctg ttccgtggcc tgctgcctcg atgccgatcc tgaagcctgc gcctggggga
     3061 aaagttctcc ccgggccgcg atatagcgtc cgctgtattc ttcaatccgg gccgccgcga
     3121 tgagcacgtc tttgttttcc tgcagggccg tttccatgag agcgttgagg acgggatcgc
     3181 gaagttgctc ccaccatttc aggttgacca gcgtccgcgc ttctttgtct tcaaagcgcc
     3241 attttcccgg aacggaaacc tccggacggc ggtagtccgg gccgacggcg cagccggcga
     3301 tcaggaggat gagagagaaa aggaagagtt tcctaatcat gcttttcctc ctgggcctgc
     3361 ggttgcggaa agagcggctc cgtttgcggc gctccttttt gctttccgga atggccaaac
     3421 ctttgcgtca gcttttccag aaggaagtag aacatcggaa taaagaaaac ggcgatcagc
     3481 gtcgcgccca gcattcctcc gatcacgccc gtgccgatgg agtggcggct gttggcggaa
     3541 gcgccgctgg cgacggccag gggaacgcag ccgaggataa aggccagaga ggtcatgacg
     3601 atggggcgca ggcgcatttt cgacgcctgg acggcggcgt cataggcgtt gaatccctgt
     3661 tcgcgcttca gcacggcgaa ctcgatgatc agaatggcgt ttttcgcggc cagggcaatc
     3721 agcgtgatga ggccgatctg gaagtagatg tcgttttcct gccctctgaa ccagatggcc
     3781 aggagggcgc cgcagatggc gaaaggcacg gctaaaagaa cgccgaaggg cagggaccat
     3841 ttttcatact gggcggccag gatgaggaaa accatgatca ggccgaagac aaacaccacg
     3901 gcggaggtgc cgcccgcctg cttttcctcg aaagcctctc cgctccaggc gtagccgaag
     3961 tcttcgggaa ggacttcgtc ggcaacccgt tccatggcgg caatggcgtc gccggagctg
     4021 tagccggagg cgggattgcc gatgaccttg gccgccggaa aattgttgaa ccgggtgacc
     4081 aggtccggac cggagacgtc ttttttcttc accagggctt tcagcggcac catgttgccg
     4141 cttctggatc ggacatagat ctggtccatg tctccggggc gggaacggta ttccggctct
     4201 gcctggagga tgacctgcca aaggcggctg aatttcgtga actggctgac gtaggcggag
     4261 ccgaaaagga tctggagcgt gttatagacg tcttccaccg ggacgcccag gttttctgat
     4321 ttttcccgat ccacatccac caggagctgt tgggaatgga cgttaaatag ggttgccacg
     4381 ccttggagtt caggctgttt ttttgcccgc gccacgaaat ctttggtgat gctttccatt
     4441 cggttcatat cgcctgttcc gcggttctgg atccagaatt caaaaccggc ggtggttccg
     4501 aggccgggga tggacggcgg gttcaggggc aggacaaacc cctcccggag gccgaggaac
     4561 attttggagg tttgatcgat gaccgccggc gcctgcagat tcttttcctt gcgtttttca
     4621 tagtccagaa gagaaatgaa ctgcaatccc gcgttggttt ttacctggct gtccaggagg
     4681 ctgtagccgt ccacctccac gacattttca atagccggct gctttgcgta gatgcctctt
     4741 gctttttgat tcagatcctg ggtgcggtcc aggctggcgg cgtcgggcaa aatggagacg
     4801 ccgagaaggt agccctgatc ttccgacggc acgaagcttg agggaatatg cctgaacaga
     4861 ccgatcacgc aggctatcat gatgacgaag atgcaaaggg ccacggccgc ccgcttgatg
     4921 accagttgca caaccctgga atagccttcc gtcaggctgg cgaacttttc ctcgaacttc
     4981 cggaaaaacc agtttttctg accgtgctgg ggtttcagca gaatagccgc catggcgggg
     5041 gagagcgtca gggccacaat tcccgaaaag atgacggaaa tggcgatggt gatggcgaac
     5101 tgcttgtaaa gctgtcccgt gatgccgccg agaaaagcga cgggaatgaa gacggcgcat
     5161 aaaacgagca cgatggcggc gacgggtccc gtaacctctt ccatggcttt tttagccgct
     5221 tccttgggcg gcaggttgag ctccctcatg ttgcgctcca cgttttcgat gacgacgatg
     5281 gcgtcgtcca cgacgatgcc gatggccaga accatgccga agagggtcag catgttgatg
     5341 gagaatccga aaagcagcat gccggtgaag gttccgatga tcgagacggg aacggccaga
     5401 atggggatga tggtggcgcg gagactctgc agaaacagga aaacgacgat gatgacgagc
     5461 aggcacgctt cgaagaaagt cttgacgact tccttgatgg aagcccggac aaatttggtc
     5521 gtatccatgg cgattttata atcaatgccg tcggggaaac ctgtcttcaa cgtcttcagg
     5581 gttttttcca cctgctcgga aacttccagg gcgttggcgc cggcctgctg ataaacggcg
     5641 atgatcgtcg tatcctttcc attcatgcgg cagcgaacgc tgtagtcctg cttgcccagt
     5701 tcggcccggc cgacatcctt gagacgaacg atggcggcgc cctgggcgtc ggtccggaga
     5761 atgatatttt caaactccgc cggatcgacg agcctccccc tggtggtcac ggaaaaagtc
     5821 tgttccacgc gccctctggt cggcgactgg ccgattttgc ccacggcaaa ctgctggttc
     5881 tggtttcgga cggcattggc cacgtccgag gccgtgatgc ccaactggat gagccggtcc
     5941 ggcttcagcc agatgcgcat ggcgtagtcg ggactgccca tgatcgacgc ttcgctggcg
     6001 ccggggatgc gcttgatggc gtcgagaatg tacagattgg cgtaattggc gacgtaattt
     6061 tcgtcgtagc ggtcgttggg ggagtaaatg ccgatgacca tcaaaaagct gctggatttc
     6121 ttgctgacaa tgacgccctg ctgcgtcacc gcctgcggga gctggggcag ggcctggttg
     6181 acgcggttct ggacgtccac ctgggccagc gaggggttgg tgccgatgtt gaaataaaca
     6241 ttgaggctca tcatgccagc gctggagctg gtggaggtca tgtagatcat gttgtccgcg
     6301 ccgttgacct gcagttcgat gggggcggcg atgttctggg aaatgacgtc ggcgctggcg
     6361 cccggatagg ttgccgaaac ggtgacgacc gggggcgtga tgtccgggta ctgggcaatg
     6421 ggcaggttat tcatggccac cagccccgcg atgacgatta cgatggagat gacggaggca
     6481 aaaacggggc ggtcgatgaa gaaacgggag atcatgggta agcctcctat ttctttgccg
     6541 gcggcgccgg tgacttcttt gctcccgcgg ggacggcgct tccggcggcc ggtgccggac
     6601 tgacggcaac aggcgccccg ggtgacattt tgaggagatt gtccagaatg acgcgatcac
     6661 cggcctgaag accgctgttg atgaagatat cgtttccctg ccagtctccg actgccacgg
     6721 cgcggaattc cgcccggctg tcctttcccg cgatccagac aaaaaatccc tgcgcgccct
     6781 ggctgacggc cgttctggga atcaggatgc cgtcggggcg aatgcctccc agcagatgca
     6841 cgcggacgaa ctgtccgggc cggatcacct tcggggagtt gatggggttg ggaatttccg
     6901 cccggacctc ataggtcccg gtttcggaat tgtaggaagg atcggcgaaa ttgaggcggc
     6961 ctcggtgggg aaagacggag ccgtcggcca tatggacctc cacctcgaaa tcgtagccct
     7021 tgggcatttt aaaaagacct ttggccaggt tgtccattag cttgaggttt tcgttttccg
     7081 agatgctgaa atccacccag atggggtcga ttttggccac atacgtcagg aggctgctct
     7141 gtcccacgga gatgtagctt ccctcctgct gctttgcccg tccggccaat ccgtcgatgg
     7201 gggacgtgat cgtggtgtag ctcagtttca gctccgcgtc ctggacgttt ccctgggcgg
     7261 aaaagacggc ggcgcgggcg ctcttttccg cgctgacggc gtcatccagg tctttctggc
     7321 tgacggcgtt tttctccgcg aggggacgga ctcttttcag cgtggcttcg gcgttgacca
     7381 gcttggcttc ctgctgggcc agcgcgccac gggcctgctg gagagaagct tcgaacggcc
     7441 ggcggtcgat ctggaacagc gtttgaccgg accggacgcg tccgccttcc tcataggttt
     7501 tcttttccag aaagccttcg acgcgggcgc ggatttccac ctgatgggag ctttccgttg
     7561 tgccgacaaa ttcaatgtcg atggggacat cccggggcgt gaccgtcatg accgtgacgg
     7621 gaatggcagg ccgggcatgc tctttttcgc ttttgtcgcc gcatcccgaa agggcggcgg
     7681 aaaggaggat cgttaaaccg agaaaaagga tcggagaaac aaacaaccgg agcgggcgac
     7741 ctgtgccgtt catgaagcaa cctccatggg tgtagaaaat taaagcaatt caggtataag
     7801 aaaattcagc aggcaagtca atgattttat aatgaatggt ctgatgatgc cgcgccacat
     7861 gatcaggttt gatcgtcgtg cagatttttt agcggcgcct tgcatcgcgg atcccgtatt
     7921 gcgggttttg cggggatggc tttaaatccg aatatgtagt gcgcagcttt agctgcgctc
     7981 tatcgcgcgt gggtttgccc ccgcttgcgg atataaatgt tcgtgattca tttccgccac
     8041 tccgtttcac agcgaccaga gtacagggaa ttgtccgtgg tccttaaggt tcggtttgtt
     8101 gcggcggtta aaatcacttg cgtttttttg ttcttcttca cctcagaatg gatgtcagga
     8161 tcttgtatgc ctgctttagt tgttcgcctt ctgcctcgtt tatcaatgct atgatgagac
     8221 tttttctttt ggcgacatcc tcgatctgga cagcgttgaa gatttctccc aagtccactc
     8281 ctaatgaatg agataagttt atcagggtgt tcagggtcgg attttctttt ccccgttcaa
     8341 tactgcttaa atattttgga ccgatgttca tttttgcggc aagttcctct tgggtcaaac
     8401 caattttctt tcttatattg ccaattctga tgcctatcag ccgctttgca tccaaatttg
     8461 catccacgtt catattcccc cttatttgct atgattacag aataaagagg gagcaaaaac
     8521 tatccattac cattatatag cttgacacat tcatggcatt gggtgtattt cttcgccgaa
     8581 actagattct accgcaccga caaacacatt tttggatatg acttgatcga ttaagagcag
     8641 ggcatatgtc aaaacgcatt ttgtgcttat tcatcatttt caccatgtcg ctatgttttc
     8701 tgtctccttg ctcgcttttt gcgcaggaca aaattgttgt gggttttggc ggtttatgga
     8761 ccgccggacc ggagacgaac taccctgttt attcccgcat caaaaaaaag catccgggca
     8821 aaatggccag ggccgtaacc aactctgtcg agagggttac ggtttaaaaa caatataatg
     8881 gagaaaagat aaccatgaaa acaaaacatg ttttgcgcac aggattttta ttattcattg
     8941 taattttctt tctcaatgtc gctcacggcg ctactgttca acaggccacc ggcgcaggca
     9001 caagcaagga acaagctctt tacgccgcca tgcgtcaggc ggtggaaaag caagtgggcg
     9061 ttcacattca atccaatacc atcgttatcg atgcggctgt tctggaagac aaaatttttt
     9121 cccacagcca gggttacgtt accaattata aaatcatcag agaagataaa accagtgacg
     9181 gctattcggt gaccattcaa gcctatattg atcaaaagtt agtgaaagaa gatgttaacg
     9241 ctctggccat cctccgaaaa ggcatcggta atccacgcat tctggtggtt tttaagccgc
     9301 gggcagaagg aagctcggca atcaaaggca agaattttgt tgaaaaaatc ctcgatggta
     9361 tcgaggacgc tttttccgaa aggcagtttc gtcttgtgga caggaacgct gcggcagctt
     9421 ttgcaagtca acttgaacag acgcacgaca tcgatacaga aaataataag gccgctgctt
     9481 atggtctgaa atacaatgct gaatatactt tattttatag caccgatgga gaaatcaccg
     9541 aacgcggcga ttcgtttgcc gccaatctac acgtcgatgc gagactgata gataatactc
     9601 tgtcgcagct tattaccaac aaaacagtgg atgcttcagg cgagggacga acagcagaaa
     9661 atgcgctgga aaaggcggga cgcgccggcg gcaaaaatat tgtggcgccg attatttcgg
     9721 ttcttcagcg tcactggatg gacatgcagc agaacgggtc gctttatatt gttgttctgg
     9781 acggagtgga tgatgcagat gaaatcgcgc aatttaccga aatgtttgag aaatatccaa
     9841 gagtgaacag cgccagggaa gtggaatcgg gcggcggcaa aaccactttc gaaacggcct
     9901 atcgcggaaa aagggatgag ctcgatagag atgtgttgcg gactgcgaaa tatttgggct
     9961 ggaaactaac gaaagttcgc gctgaaggcg cccgcactac atggaaaaaa caatgataat
    10021 aaggagtata tatgaataat ttttctcgcc tgccgcttgg cgttgttatc gtcggccttg
    10081 tgtttttgat gacatcatgc agcacagccc agacagttcc agaacgaacc atgactctct
    10141 cggagttttt tgccgtgccc tccagcaagt tatccagcac ggcggacagc attgttgtgc
    10201 ggggcgcgcg cactgctttt ataaaaggtg tcatgagagt ggatttcaaa atttataacg
    10261 atcgcggacg acgcaacatc gtcaattacc ggatgcgctg gttggataag gatggcctga
    10321 tggcggctcc ctatttggat tggacaacca ttgctcttga aggccagcaa gaaattgtga
    10381 ttactgcagt ttgtccgaac aacaaggccg tggactatat cctggagcta cgaacaaact
    10441 gatctttgcg tataaattat catcataagg ggggaaaaaa taaattatga aaagcaaact
    10501 gattctgcct gctatcctgt gcctgtgttt tttaacgccg ctgatcgttt ttgcggaaat
    10561 gcatgaattt cagggcgaag gcacggctcc gatcaatccg gacaacatct cgtcaacgcg
    10621 catggcggca accaagaacg ccaaacgcag cgctattaat gcagccatca gaaaattcat
    10681 cggacctact gcggccaatg atcccaaggt gaaggaagca attgaaaata tcctgacgca
    10741 gatcgacgac agcaaaatta tcagtcgcaa ggcagacaaa gatggagata attatgtttt
    10801 aaagataacc ctgaaagttg atgatctgga gttccggcga ttgttgcaaa cagaggggat
    10861 tgccccatcg acggatcgca cgtccaaaat attggtgttg atggatgaat accataccac
    10921 acccaccgat atgcaaaaac cactcaagga agtggtagaa tattcccacg ataaaacagc
    10981 aactgccgaa gccagtcttt ccgcggcctc cagcgcgtct tctgaaacga aggcggccgc
    11041 ccgggaatca agcgccagtt cggctagtta tgcagccaaa gctcgatatg gggctcatgc
    11101 cagcggacac ggatattcgg tggggggaca cgccagcggc caggtagcgg catccggcgc
    11161 acagcgctcg caaagctccg ccgattattc tgataaatca tccgaatccg ccagcctgga
    11221 agcaaaatca ttcgaccagc agaaagatgt cgtcaatttt aagaaactgg tggagtacca
    11281 gccgcgcaat gtgggaccgg ccaagagcaa cgccacttat aatgctctgc tcaacatggc
    11341 acagcaatat gatttaaatg ttatgaacag cgccgtattt cgcagtaaat attttccgaa
    11401 taaatctgta acccttgccg atctggccga cggctcagaa cttggcaaac atgttcaaca
    11461 ggctggaaaa gaaggcgccg agtatttcat gatgggcaat gccatcatgc gtgatctggg
    11521 aaacaatcaa tgtgacggcg atgtaaccat atctgcctat tctgtggacg atagcactaa
    11581 tctgacagcg gcaactcata atgaaagcgc gcgcggaaca agccctgatg attgccgtgt
    11641 gacgctggct aaaaaactgg cccattttgt agggaaaacc atcggcagtt ccatcaagga
    11701 ctacaacagg cagcgcgagg tatccggcaa ggaatatcga gtattattgt taagctcggc
    11761 agatgggctg ggtggcagaa tgataacttc attttccaat aatctgttaa aaattaaagg
    11821 cttgaacagc aaactaaacg aaagagaaaa atcttcttct aagtatgatg tggttttaac
    11881 atacaaaggc gatgtccctt ttagcaacct aataactgag gttctcgaca gcatgcccgc
    11941 tatgaaccag gcggattttg acgtagcagg cacaaccatt accatctgcc tggaggggaa
    12001 aaaccgttgt aaatagttaa atatatgcat cctgtttttt tgctgaccgg caaattaaaa
    12061 ccagattcgt gtgtgtgaat caactatgag aggttttatg aaacgcttaa tttttatagt
    12121 tattttgctg tctatttgtc tgctgcccac cgtgccggaa gccgcgccaa aagtttatcc
    12181 tgtaaaaaga gtcttcggct ttcgtcaaag tactctggca aatgcgccgg cctttcaaaa
    12241 atgggttgcg tcccggagcg ccgcgggaga aatgcgtgaa gcccgcagca ttctctccaa
    12301 agaatttgaa gcgcagttca aaaaaacttt tggttctctg tccgcggaca acatcacgga
    12361 tgtaaataaa cacgaagtgc ttgttgcttc tttgcacctt ttgcgggcat cggaatacac
    12421 tgtgcccaaa attggatttt ccgaggtttc catgcccatc accttgagca ttatcatcac
    12481 caatccggcc acgggagaag ctctgtattc gtttaccaaa acagcttacg cgaccacgcg
    12541 ggttgtaaaa acaaacggcg gcaataagga agtcctatcg caggaattac catctcaaaa
    12601 tgccggcagc caggagcagt tgctcgcggc ggaaaggaat gcacaggagg cagaggaaga
    12661 tagagagatg tttgttaatg cccgtcccag cgaccaggaa caggcccaga tgattgccaa
    12721 ctctggggct aactatcagg ccctggttgc gtctctcctc gaagaagcaa aagccggcta
    12781 caatccgtca aacatcgaaa tcaaaatagc ggatatctgg aagggttttt atattctgga
    12841 caaaggcagc aagttcggaa tcgttaaagg cgataacata acagacgctt ccgggaatga
    12901 actgacggta gtccatgcgg cggaaaatta cgccgtcgcc cagagtctct tgggtaaagt
    12961 tgacagggac agaaagtttt ttaaatacag cacggggagc gccgccgggc agtttaataa
    13021 accacgggtc ttaaccatgc atgagggatg gagagatccg attttaagag gaatatccta
    13081 tatttttgat tcccaggtac ccaaagaatc tgcctttaca cttcttccgg taaacgaaca
    13141 ttttaccaga ctgcttcatt gcgttgccca ggacacatat gccggaaagt atgaaactac
    13201 caaccagcgg gccatgccga attatctgat aaaatttagc gcaaccaaac cgcgatttta
    13261 caccatcagt gaagagggaa aattcagtca gcacatctat gagcaaagcg tggtgggcga
    13321 gcttctggac aggcagggtc gggttttgtt ttctgccgtg gggacagacc ggattgaaga
    13381 tgaaaatgta gccggtatgg tttttgccgt aaaggatcgg ctggaaatcc tgctaaaaaa
    13441 tgccacggta caactggcaa agcagttctc aaacgccatc aagttttcgc gttttgagtt
    13501 gccggtgacc ggcattgacg gcaaggttat tgaaataaaa gataaatcaa tggatttgcg
    13561 catcggaaag gatgttgtca tttatcgcaa tatcgggagt attaaaggca ttgctacgga
    13621 cgttgttgtg ccgctttggc aagctgctgt agtggatgtt cagaatgata cggtaaaact
    13681 caacctgatc atgaagctga cggatgaaga gatttccgtt tccacggatg acctggtcat
    13741 tatggatgct gtatccgccg ccggcgctgc caatcaaagc aatacgtcca taaaatactg
    13801 cacacacgaa cgatcgcagc ggggaccgct ggcaattgac gattttgaag ttgtatcaag
    13861 agcatacggt tatctgtcgc cttacacttt atataatcaa gacgaggatt ttaccaaaaa
    13921 aattcgggat gcggcaaaat tcggcggatt cagggatacg ctggaactga ataagacaga
    13981 cacggcgggc cgctgcttac tgccggttta tcgggcaagc aggggagaaa aagggacaga
    14041 tcagacaatc atcaactgcg acgaaaatgg attttgtact gacaccatag aaatcgctgt
    14101 cggttatcgg ttgtatatca ataatgaaag aaagggggga tctgcatcta aaaccaaagt
    14161 gaccgtcaaa gactgtaaac gcgatgtttg tgcgccggtt atgcagagcg aattgagcaa
    14221 aattaaccgc gacctgctca aggcgaatat tgttcaaatt atttttaaac aaggagaatg
    14281 aaaaatgagg ctaaatcgag ttattattgt tggaatcctt tttgctgtgc tgtcattggg
    14341 tacggcatac gccggacttt ttgacgctgt gacatctata gccggtggcg ggtcttcagg
    14401 tggtgacgat cttgatacat ttcttgtgag agcaaaaacg gcagaagaat tgataaataa
    14461 tagcacaaaa tcaatgttta aggccatcgc ttcagataag gaacaggctc aacttgaagc
    14521 aaagcaaaaa aagctaaacg aaacaactga tcccaaggaa aaggaagccc ttcagcgcga
    14581 attaacagaa agtcaattgg cagtaataaa aaaagaggca ggtaacgaag aacttcaaaa
    14641 agaagcctcg aaatggagtg acaaaaaaaa ggaacatgtc gccaatgcat tttttaacta
    14701 cgcattaggc gctttgcagg ccggtttaat tgtcaaggac ggaaaagccc ttgtgaccaa
    14761 tccggccaac gctgtgaaag cggcaacaaa attaggggaa gttaaagatg caatcgcatc
    14821 gcttggtgga attgcatcag gctcggttac tatgatcagc tcgctgaaaa cattcatgtc
    14881 tgctgcaaac atatcggcga cgatacccaa aaccgcaacc gacaaaccta aaccgcaagc
    14941 tgacgtagac gattaataag gaagataggt tttcccgact catgcatggt tagcatctct
    15001 accaatcctc tggttttcag acatgtgttt g
//
LOCUS       DAOSRM010000053        14840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4125_length_14840_cov_111.538181, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000053 DAOSRM010000000
VERSION     DAOSRM010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 14840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14840
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4125_length_14840_cov_111.538181"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1234
                     /locus_tag="PLA58_06420"
     CDS             152..1234
                     /locus_tag="PLA58_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOR62466.1"
                     /translation="MAKIDAFFQLMHEQGASDLHLVSGQQPVIRMRGDLERIKYDVLT
                     NDGLKALLYEITPEHKIKVFEETGDVDFAYEIPGLARYRANFFQQKYGVAAVFREIPS
                     KILTCEQLALPPVLSKLAQLPRGLVLVTGPTGSGKSTTLAAIIDEANRTRKDHIITVE
                     DPIEFVHQSQSCVVNHREVGVHTKTFSSALRGALREDPDIILVGELRDLETISLAVEA
                     ASTGHLVFGTLHTTSAPKTVDRIIEVFPSSEQAQIRSTLADGLRAVIAQVLFKRVDIK
                     GRCAALEILIATSAVRNLIREAKTFQIPSMMQTGKKYGMQLLDDAIMDYVSKGWISPD
                     EAYNKANDKAKFRPLLKSPPSDFTEA"
     gene            1253..2446
                     /locus_tag="PLA58_06425"
     CDS             1253..2446
                     /locus_tag="PLA58_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOR62467.1"
                     /translation="MRKQEIDHILEHMLASHPNISDLNLTVNKPFQVESSGQLTGVDL
                     DPPFARLTPFQTETFALNLINQDHRLTDDLVATGSCDLSYELPGIARFRVNIFSQRGH
                     YSIVLRKLETKIPSCADLKLPEAFYSIAGEKNGIVLVTGSTGSGKSTSLAAVINEINE
                     QKSIHIITLEDPVEFTHQQKKATINQRELGKDFDTFSSGLRAALRQAPKVVLVGEMRD
                     RESVEIALSAAETGHLVLSTLHTVDAGSTINRILGMFPSEEENQIRIRLADTVRWIVC
                     QRLLPKEGGGRVAAFEALGMNLRVKDIIQNGESEGKTFYDVMEQSKAFGMITFDTCIV
                     DLYSKGLISEETAKAYASNKSNVNRGIDSIKNARGEKTTDIGKLEVDKSYGKPQASVP
                     PWQKK"
     gene            2484..2984
                     /locus_tag="PLA58_06430"
     CDS             2484..2984
                     /locus_tag="PLA58_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62468.1"
                     /translation="MAEERAAKAGDMSEGRPFGYIAPEGNTAMVCESDGAVRDKIISE
                     LKGMGYAVAEAKSAREALQFMRFHIFSAIFVDESFDTAEGQANSVLQFLEGLSMTVRR
                     QTFVTLLSASLKTMDNMMAYNRSVNLIVNKNEIADIGQVFKKALDEYESFYHVYKENL
                     RKAGKA"
     gene            complement(3162..4109)
                     /locus_tag="PLA58_06435"
     CDS             complement(3162..4109)
                     /locus_tag="PLA58_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR62469.1"
                     /translation="MTTHPATQPLIDEADRLLRQEKYGEAVALLEAFHSRHPDQEPVL
                     LRLAWASWDSGNKKQSLEYWEMLLERELKRRVFTGFAYDELVRIYKQEALFEKLVAVC
                     EKAVAVQPDDVCLLTELGNAQLQAGRPLEACRIFEKLTRIEEDHPDYWRRLGEALFAA
                     GRAPDGEAAFLRAGSLDPEQMDRYYFRIADLYMRDKRHDEAMRFLKRCISFNPSQPLY
                     SCSLGDELISLGRIDEAEAVYETAAAQSPSGAYYNRLGNALMKEKIFDRAACAFQKAV
                     SREALRPFYINLARAYHEMGQLDQAAEALRQADAVSGTV"
     gene            complement(4153..4347)
                     /locus_tag="PLA58_06440"
     CDS             complement(4153..4347)
                     /locus_tag="PLA58_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62470.1"
                     /translation="MALDEPKNDDEVIDEKGTKFVIEKDLFSQAKPINIDFITTPQGA
                     GFKLTSSLAAAGGGCGSCSC"
     gene            4671..>6023
                     /locus_tag="PLA58_06445"
     CDS             4671..>6023
                     /locus_tag="PLA58_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOR62471.1"
                     /translation="MLKSLKAALIASLLFAALPASAENFTVMGEMDSTIHYELQHQIV
                     AGDSMRKLVMSFVEPSSFDSPTYSQKISNFKITFTPDAQEKKTLKDARGNKTIQATWT
                     DVPKSIDAVVSFDAQTSTGLKAIDSVAPFPLASVPGNLKDYLQASEQVQTNDPAIGEL
                     SQKLTRGSKTQFEAVQKVVSYVVDHVRYVNPPPQYDALYALRTGRGNCQNYSHLTAAL
                     LRNAGVPVRIVNGVTMNQPFNVSWEKGTLTFKMGQGRHSWVEVWYPDLGWIPYDPQNM
                     QFFISNRFVRIEVGVDNNETKNDGLVRWAQSAGARSKPTLQETIGGNFLSDATKVTAQ
                     RQAHGPKNLLLGPDVLARLEVPAAPEPTPAPPVPAPPVPAPPVPAPPVPAPPVPAPEP
                     PKPTPPAPVPPVPVPPVPTPPVPAPPIPVPPVPAPEPPKPVPEPPKPVPPVKPPKAWE
                     Y"
     assembly_gap    6024..6123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6124..6748
                     /locus_tag="PLA58_06450"
     CDS             <6124..6748
                     /locus_tag="PLA58_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62472.1"
                     /translation="PEPPKPVPPVKPPKAWEYKMPFVFGNLEYPENVDFAFPRETKAK
                     GKDSFEMSRNFLVETSEYVTTNATQYAQVVELAKPVTIDQVGLALHKFGGEGWIWVDI
                     FKDQDGKPGDILHTTQMISLEDISGKPGYRWVDFKFSDKEKPVLMPGAYWIALGFSGT
                     PIMNWFYTYGKPVGPVYGTRYKSVFAQDWSGALNYEFNYRVVGMTVK"
     gene            complement(6749..7339)
                     /locus_tag="PLA58_06455"
     CDS             complement(6749..7339)
                     /locus_tag="PLA58_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR62473.1"
                     /translation="MLKTMKPQHPQGDLTLFLQALNFSAGKHRDQRRKDSAASPYINH
                     PIEVACLLWTVGGVHDEAAILAAILHDTIEDTDTTPEEIRTRFGDDVLGLVLEMSDDK
                     SLPKQERKRKQIVHSPRLSLRAKQIKLADKICNVHDIAFSPPEGWPLARRIDYLQWAR
                     EVIDGLRGANALLEERFDETLVLARQKLASESDGSP"
     gene            7468..8370
                     /locus_tag="PLA58_06460"
     CDS             7468..8370
                     /locus_tag="PLA58_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62474.1"
                     /translation="MNDERILFEKPDLALLRKDHAVFDMHFHTCRTDGRDSVRAIAHR
                     ARILGVGVAITDHNDIESALEIAQYRDVPSIPGIEITSREGTHILVYFYESRHLKKFY
                     TKYIQPFLGREIMSSTRLSMEEIVHSAKLFPSVTIFPHPYCVAYTGICNLNFEQARLE
                     RLLDAADGVEVINAENIHRWNLRCTLLGLYLKKAITGGSDGHSLYHMGKVVTYACCEP
                     TGPAMLDAIKNGSARVIGKEINLFRKVAAGTSKLNVPVAAYPERLGKNIRYSYRLIHV
                     KSILIRRQLQLRIYRRRNRLHPLR"
     gene            8386..9465
                     /locus_tag="PLA58_06465"
     CDS             8386..9465
                     /locus_tag="PLA58_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOR62475.1"
                     /translation="MFNKLLAGFPLLIFFLVLGAVTWLLGEILKPFFFAIFWAVLLAA
                     IFEPVNEWLRRKFKNPNVSAGLALTAVFLTMILPVGLIFSLLVGQSLDLYQSINQNGG
                     SWMQTITGFLQSVSSHPLLARFQLDHAFITDKSVELLKTFTEFLVKNLSAFTQNTVVF
                     LVKFAVALYCLFYFFRDGKQLFGSIVRHLPVNETHIHTFTSEFLVTSKAALKFTFVIG
                     GIQGLLGGLVFYFTGISNPLIWGVLMVGLSIVPAIGNAIIWAPAGIIMLAMGHVWQGI
                     AILFFGVVVISSVDNLLRPILLGKDIQMHPLLIFLSTLGGIAVFGFSGFVLGPVVASF
                     FLASWKLFLDLSSEAKTDSSGGETG"
     gene            complement(9503..10126)
                     /locus_tag="PLA58_06470"
     CDS             complement(9503..10126)
                     /locus_tag="PLA58_06470"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOR62476.1"
                     /translation="MKLKTTGQVSGMSILERAFYARPTITVARELLGKKLVRQTDGAK
                     LSGVIIETEAYCGSSDSACHAHRGKTPRNTVMFGRPGVAYVYFTYGMHYLLNLVTEEE
                     GNPCAVLVRALLPLSGLEEMQSRRKKHGKELTNGPAKLCQALAIDKSLNGWDLTLGER
                     LWVENDQSVQTRDILSTPRIGIDYAREKDRKAPWRFLIRPGAETQRL"
     gene            10310..11260
                     /locus_tag="PLA58_06475"
     CDS             10310..11260
                     /locus_tag="PLA58_06475"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOR62477.1"
                     /translation="MSEAKGTAAVFPGQGSQRQGMGKDFYDAIPVCRATFEEASDALG
                     LDVAAVCFEEDARLHLTEYTQPCLVTTETAMFRGLAERYGWKADCFGGHSLGEFSALV
                     AAGVMPFADAVRIVRTRGRLMQEAIPVGVGAMAAIIAEGIDAGTLSTALADLPVDIAN
                     INSANQVVVSGEAAALPEAGNRCKDLFSGPKSCRVVPLNVSAPFHSRFMKKIEEPFRE
                     ALKMISGSWDVQNAPAVTSNYRGGFHPADADGIADNLVHQLSSSVLWRDNMQALAGRA
                     GAIYEIGPGRPLRDFFKTIGVTCESVINLAAAEKIFGGVS"
     gene            11301..12500
                     /locus_tag="PLA58_06480"
     CDS             11301..12500
                     /locus_tag="PLA58_06480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR62478.1"
                     /translation="MDLGFSENQLLYVDTVRRLVKNEISPRILEMEKEHGFPWAIISK
                     AWEMGLVNLCIPESIKGFDVDVFSSALIIQELSYGDTGIATSAMCNDLANVVISRHGT
                     DQQKERFLRPFVEKPLLASFCLTEPGAGSDNSLMTSFIAPRDDGSWVLNGSKCFITNA
                     SYASQFTVLCKNGKPSSNFMACVVVPAESVIAADLPDVGYSTREIQLSTGGRIVIGKP
                     EDKLGQRLSNTASVTFEDVIVEPDQIIGNRRFGFKYIMDVLDFARPMVAAIGLGLAKR
                     ALDVTMDYTRQRKQFGQRICDLPVARDTLVAMWKKVELAELSLWKACWKIQKNDKDAG
                     IFASLAKNTCAEAALFCANEGLHLHGGYGFTADYEISKLARDAHIIDIYEGVREVQNM
                     IIGREIV"
     gene            12511..13278
                     /locus_tag="PLA58_06485"
     CDS             12511..13278
                     /locus_tag="PLA58_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOR62479.1"
                     /translation="MKIQDVIALVTGGASGLGGATVLELAARGAKVVIVDLDVEKGEK
                     LAGEAGENVMFVRCDVTGEDSVRQAIQQSLDRFGSITAAVNCAGVPDAAKVMGKKGPL
                     PLSLFNRVISINLVGTLNVIRLAVEQMVNNAPNEEGERGVIVNTASIAAFDGQIGQAA
                     YAASKGGIVGMTLPIARECADYGIRVATIAPGLFETPLMAGLPPAVKEDMAKGVPFPR
                     RLGRPAEFARMAVHILENPMLNGCCIRLDGALRMPPR"
     gene            complement(13289..14032)
                     /locus_tag="PLA58_06490"
     CDS             complement(13289..14032)
                     /locus_tag="PLA58_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOR62480.1"
                     /translation="MFAWIASPEAWIALATLTALEIVLGIDNIIFISILVGRLPAQKR
                     SFARNLGLMLAMVTRLALLFSIVWVANLTQPWFTLFGQDISGRDIILIGGGLFLLAKA
                     THEIHSSLEGVEEAKPKLKTASLAPILIQIALLDVVFSLDSVITAVGLARHVSIMAIA
                     IIVAVGIMLVAAKPIAEFVDAHPTIKILALSFLILVGVTLMVEGFDVHVPKGYIYFAM
                     AFSVAVEMLNIRMRKKQTDPIQLRKEITG"
     gene            complement(14051..14650)
                     /locus_tag="PLA58_06495"
     CDS             complement(14051..14650)
                     /locus_tag="PLA58_06495"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOR62481.1"
                     /translation="MKKTDKSHASVKKVAARKKPTTTSAPAERGLVIVITGNGKGKTT
                     SAFGQALRAVGQGYRVYIMQFMKGRNYGEYTAGSRYLPSLTIRRSGLDSFVMRGSPAP
                     VDIELARKGFESAKKAIASGRYDMVILDEINVAVDFKLIELADLIRLITDKPPGLDLI
                     LTGRYAAKEVIRLADTVSEIKDIKHHYAAGIKGRAGIEY"
BASE COUNT         3106 a         4148 c         4226 g         3260 t
ORIGIN      
        1 atgagagctt tgaataaagg ccatttttgt catttcgacc gaagggagaa atcttgaaca
       61 aacggccatc cttaagattt ctcgtcgctc acgctcctcg aaatgacatt tttcaaagct
      121 ctcaaaataa aacgattgac gggagatatt tatggcaaaa atcgatgctt tttttcagct
      181 catgcatgaa cagggcgcat ccgatctcca tctggtttcc gggcagcagc ccgtgatcag
      241 aatgcgcgga gatctcgaac ggattaagta cgatgttttg accaacgacg gcttgaaggc
      301 gctgctgtat gaaattacac cggagcataa gatcaaggtg tttgaagaaa caggggatgt
      361 cgattttgcc tatgaaattc cggggctggc ccgataccgg gcgaacttct tccagcagaa
      421 atacggcgtt gccgccgtct tccgtgaaat tcccagcaag attctgacct gcgagcagct
      481 ggcgctgccc ccggtgcttt ccaagctggc gcagttgccg cgcggactgg ttctggtgac
      541 cggaccgacc ggatccggca aatcgacgac gctggcggcc atcatcgatg aagcgaaccg
      601 cacccgcaag gatcatatta tcaccgtgga agacccgatt gaattcgtcc accagagcca
      661 gagctgcgtc gtcaaccatc gtgaagtcgg cgtgcatacc aaaacattca gctcggcgct
      721 gcgcggggcg cttcgtgaag acccggacat cattctggtc ggcgaattgc gcgatctgga
      781 aacgatttcc ctggccgtgg aagcggcgtc caccggccat ctggtgttcg gcaccctgca
      841 cacgaccagc gcgcccaaaa ccgtggaccg catcattgaa gtcttcccct ccagcgaaca
      901 ggcgcagatc cgctcgaccc tggccgacgg cctgcgggcg gtcatcgctc aggtgctgtt
      961 caagagggtg gacatcaagg gccgctgcgc cgcgctggaa attctgattg ccacgtcggc
     1021 ggtgcgcaac ctgatccgcg aggcaaagac cttccagatc ccctccatga tgcagaccgg
     1081 caaaaagtac ggcatgcagc ttttggacga cgcgattatg gattacgtca gcaaggggtg
     1141 gatcagcccc gacgaggctt acaacaaggc caacgacaag gcgaaattcc gtccgcttct
     1201 gaaaagtccg ccttcggact ttaccgaagc ataaccgaag cgaggtgggt ttatgagaaa
     1261 acaggaaatc gatcatattc tggagcacat gctggcgtcc catcccaata tttccgacct
     1321 gaacctgacg gtaaacaagc cttttcaggt ggaatcgtcg ggacagttga ccggcgtcga
     1381 tctggatccg ccttttgccc ggttgacgcc gtttcagacg gaaaccttcg cgctgaacct
     1441 gatcaaccag gatcatcgcc tgaccgatga tctggtggcc acgggctcct gcgatttgtc
     1501 ctatgaactg ccgggaatcg cccggttccg cgtgaatatt ttttcccagc gcggccatta
     1561 ttcgatcgtt cttcggaaac tggaaacgaa gattccgtcc tgtgcggatt tgaagctgcc
     1621 ggaagccttt tacagcattg cgggtgaaaa aaacggcatc gttctggtga ccggttccac
     1681 gggcagcggc aaatccacgt cgctggccgc cgtgatcaat gaaatcaacg agcagaaatc
     1741 catccacatc atcacgctgg aagatccggt cgaattcacg catcagcaga agaaagccac
     1801 catcaaccag cgggagctgg gcaaggattt cgacacgttt tccagcggac tgcgcgccgc
     1861 gctccgtcag gcgcccaagg tcgtgctggt cggtgaaatg cgcgaccgcg aatccgtgga
     1921 aatcgcgctg tccgccgcgg aaaccggcca tctggtgctg agcacgctgc atacggtgga
     1981 tgccggctcc acgattaacc gcattttggg catgttccct tccgaagaag aaaatcagat
     2041 ccgcatccgc ctggccgata cggtccgctg gattgtctgt cagcgtctcc tgcccaagga
     2101 gggaggaggg cgtgtggcgg ctttcgaagc gctgggaatg aatctgcggg tgaaggatat
     2161 tattcagaac ggcgaatccg aaggcaagac cttttacgat gtgatggagc agagcaaggc
     2221 gttcgggatg atcacgttcg acacctgcat cgttgatctc tattcaaagg ggctgatttc
     2281 cgaagaaacg gccaaagcct atgcctccaa caagtcgaac gtcaaccggg gcattgactc
     2341 catcaagaac gcccgtgggg agaaaaccac ggatatcggg aagctggagg tggacaaatc
     2401 gtacggcaaa ccccaggcca gcgttcctcc ctggcagaag aaataaatga cgatgccatg
     2461 aatttcaagc gaaggagatg ataatggctg aagaaagagc ggcaaaagcg ggggatatgt
     2521 ccgaaggacg tcctttcggt tacatcgcgc cggaagggaa caccgccatg gtttgtgagt
     2581 cggacggcgc cgtccgggat aaaatcatca gcgaactgaa aggcatggga tatgcggtcg
     2641 cggaagcgaa gtccgccagg gaagcccttc aattcatgcg gtttcatatt ttcagcgcca
     2701 tctttgtgga tgaaagtttt gacacggccg aaggccaggc caacagcgtc ctgcaatttc
     2761 tggaaggcct gtccatgacc gttcgccggc agaccttcgt gacgctgctc agcgcgtcgc
     2821 tcaagaccat ggacaacatg atggcctaca acagaagcgt caacctgatt gtcaataaaa
     2881 atgagattgc cgatatcggc caggtattta agaaagcttt agacgagtac gaaagctttt
     2941 accatgtgta caaggaaaac ctgcgcaaag cgggcaaagc ttgacggcct cgtaaaaagc
     3001 ccggatgctg cgttgcgctg catcctttcc cggccttcgc ctccggcagg cttattgcgg
     3061 cgtccgttga agcacgcctc attcctctgg atttgcgcgc cttgcctgcg gagcttttta
     3121 cgaagccgtc taaacttacc attttttcga cttcatcaag cttagacggt tccgcttacg
     3181 gcgtcggcct gccggagtgc ttccgcggcc tggtcgagtt gtcccatttc gtgataagcc
     3241 cgggccaggt tgatgtaaaa gggccgcagg gcctcgcgcg aaacggcttt ttgaaaggcg
     3301 caggccgcgc ggtcgaagat tttttctttc atcagggcgt ttcccagacg gttgtaatag
     3361 gcgccggaag gactctgcgc cgcggcggtt tcatagacgg cctcggcttc atcgatgcgc
     3421 cccagcgaga tcagttcgtc tcccaggctg caggaataca gcggctgtga aggattgaag
     3481 gaaatgcacc gcttcaaaaa ccgcatcgct tcgtcatggc gtttgtcccg catatataaa
     3541 tccgcgatcc tgaaataata gcggtccatc tgctccggat ccaggctccc ggcccgcagg
     3601 aaggccgctt cgccgtccgg cgcgcgcccg gcggcaaaaa gcgcttcgcc cagccggcgc
     3661 caataatcgg gatggtcttc ttcgatccgg gtgagttttt caaaaatacg gcaggcttcc
     3721 aaaggccggc ctgcctgaag ctgggcgttg cccagttcgg tcagaaggca gacatcgtcc
     3781 ggctgcacgg cgacggcttt ttcgcagacg gcgaccagtt tttcaaaaag agcttcctgc
     3841 ttgtaaatcc gcacaagttc atcataggca aaaccggtaa aaaccctgcg cttcagttcc
     3901 cgctccagca gcatttccca atattcaagg gattgttttt tgttgccgct gtcccaggag
     3961 gcccaggcca ggcgaagcag gacgggctcc tgatccgggt ggcggctgtg aaatgcttcc
     4021 agcagggcga cggcttcgcc gtatttttcc tgccggagca ggcggtcggc ttcgtcaatc
     4081 aacggttggg tggcaggatg ggtcgtcatg atacaaaaaa acgggggaga aaattctccc
     4141 ccggaatgtg gttcagcagc tgcacgaacc gcagccaccc cccgccgcgg cgaggctcga
     4201 tgtcaatttg aagcctgctc cctgcggggt tgtgatgaaa tcaatgttaa tcggttttgc
     4261 ctgactgaag aggtcttttt caatgacaaa ttttgttccc ttttcgtcaa tcacttcatc
     4321 gtcgtttttc ggctcgtcca gagccatgcc cagagagggc ccggatcaac cgatggacat
     4381 ggtgatgcgg atggccgcat cagcgctttt gtccttgaga aaatccttga tgacctccga
     4441 ggccttgtct gtaactgtca gcattaagta tcctccttac gggaatgcct tttgcggcat
     4501 ccaacttggg actaacttat tcataaaccg tctgttgtca attatatttt tgccggggat
     4561 aattaagtgt ctgatttcaa taaagaaaag aacattttgt ttttattgac attcccggcg
     4621 cacctcattt atgatggagc cgtttaaata tccgacgggg gagacctgct atgctcaaaa
     4681 gtctgaaagc cgcactgatc gcatcgctgt tgtttgccgc tttgccggct tcggccgaga
     4741 actttacggt catgggggag atggactcga cgatccacta tgaattgcag caccagatcg
     4801 tggccggcga ttcgatgaga aaattggtca tgagttttgt cgagccgtcc agctttgatt
     4861 ctccaaccta cagccagaaa atcagcaatt tcaaaataac gtttaccccc gacgcccagg
     4921 agaaaaaaac gctcaaggac gcccgcggca ataaaaccat tcaggcaacc tggaccgatg
     4981 ttccgaaaag cattgacgcg gtggtgtcgt ttgacgcgca aacgagcacc ggtctgaaag
     5041 cgattgactc tgtcgcgcct tttccgctcg cgtccgtccc cggcaatttg aaggactatc
     5101 tgcaggcctc ggagcaggtg cagaccaatg atccggccat tggggaactt tcgcagaaac
     5161 tcacccgcgg gtcgaaaacg cagtttgaag ccgtacagaa agtggtgagc tacgtggtgg
     5221 accatgtccg ttacgtaaac cccccgccgc agtacgatgc gctctacgcg ctccgcaccg
     5281 gcaggggcaa ctgccagaac tattcccatc tgaccgcggc cctcctgcgc aatgcggggg
     5341 tgccggtgcg gattgtcaac ggcgtcacga tgaaccagcc gtttaacgtg tcctgggaaa
     5401 aaggcacact gacctttaag atggggcagg gcaggcactc ctgggtggaa gtctggtacc
     5461 ccgatctggg atggattccc tatgatccgc agaatatgca gtttttcatt tccaaccgtt
     5521 ttgtgcgcat cgaagtcggc gtggacaaca acgaaacaaa gaatgacggc ctggtccgct
     5581 gggcccagag cgccggggcg cgcagcaaac cgacgctgca ggaaaccatc ggcggaaatt
     5641 tcctttccga tgccaccaag gtaacggcgc agcgtcaggc gcacggcccg aagaatctgc
     5701 tcttgggtcc tgatgttctg gcccgtctgg aggttcctgc cgcgcccgaa ccaactcccg
     5761 cgccgccggt accggccccg ccggttcccg cacctccggt tccggcgccc cccgtgcccg
     5821 cgccgccggt tcccgcgcct gaaccgccca aaccgactcc tcctgcacct gtaccgcctg
     5881 taccggtccc gccggttccc acacctcctg ttccggcgcc ccccataccc gttccgccgg
     5941 tgcctgcgcc tgaaccgccc aagcctgtgc ctgaaccgcc caagcctgtg cccccggtga
     6001 agcctccaaa ggcctgggag tacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnngcctgaa ccgcccaagc ctgtgccccc ggtgaagcct ccaaaggcct gggagtacaa
     6181 gatgcctttc gttttcggca atctggaata tccggaaaat gtggattttg cgtttccccg
     6241 cgaaaccaaa gccaaaggca aggattcctt tgaaatgtcg agaaactttc tggtggaaac
     6301 atcggaatac gtgaccacca acgcgacgca gtatgcccag gttgtggaat tggccaaacc
     6361 ggtgacgatc gatcaggtgg ggctggcgct gcataaattc ggaggcgagg gctggatctg
     6421 ggtcgatatt ttcaaggatc aggacggcaa gcccggggat attttgcaca cgacgcagat
     6481 gatcagcctg gaggatattt ccggcaagcc cggttatcgc tgggtggatt tcaaattcag
     6541 cgacaaggaa aagccggttt tgatgcccgg cgcctactgg atcgctctgg gattttcggg
     6601 cacgccgatt atgaactggt tttataccta cggcaagccg gtcgggccgg tttacggcac
     6661 ccgctacaag agcgtcttcg cacaggattg gagcggagcg ctcaactatg aattcaacta
     6721 ccgggtggtc gggatgacgg tgaagtgatt acgggctgcc atcgctttcc gaggccagct
     6781 tttgccgggc caacaccagc gtttcatcga agcgctcttc cagcaatgca ttggcgcccc
     6841 gcaggccgtc gatgacctcc cgggcccatt gcaggtagtc gattctcctc gcaagcggcc
     6901 agccttccgg cggggaaaaa gcgatatcgt gaacgttgca gattttatcg gccagcttga
     6961 tctgtttggc gcgcagcgac aaccgggggg agtggacaat ctgtttgcgt tttctttcct
     7021 gtttgggcag gcttttgtcg tcgctcattt ccagcaccag accgagcacg tcgtcgccga
     7081 agcgggtgcg gatttcttcc ggcgtggtgt ccgtgtcttc aatcgtgtcg tggagaatcg
     7141 cggccagaat cgcggcttcg tcatgaacgc cgcctaccgt ccataacaga caggcgactt
     7201 cgatcggatg gttgatgtac ggcgacgcgg ccgaatcctt tctccgctgg tcgcggtgct
     7261 ttcctgctga aaagttcagg gcctgtaaga ataaggtcag gtcgccttgc ggatgttgcg
     7321 gtttcatggt cttcagcata ccacaggcgg ggtcttcccg caaatgcgcc gggaggagat
     7381 gcgacaggac atggcgcaga cgcgtttgtg attcccgggg atctgtgcta aacaactccg
     7441 cggacaacga aaaccggtga aatgacgatg aacgacgaaa gaattctttt tgaaaaaccg
     7501 gatcttgcgc ttttgcgcaa agatcatgcg gtgttcgata tgcattttca tacctgccgc
     7561 accgacggcc gcgacagtgt gcgggccatc gctcaccggg cgcggatcct gggcgttggg
     7621 gttgcgatca ccgatcataa tgacatcgaa agcgccctgg aaatagccca gtaccgcgat
     7681 gtgccgagca ttcccggcat tgaaatcacc tcccgggagg gcacgcacat cctggtgtat
     7741 ttttatgaaa gccggcattt gaaaaagttt tatacgaaat atattcagcc ttttctgggt
     7801 cgggagatca tgagttccac aaggctctcc atggaagaga tcgtccactc ggcaaagctt
     7861 tttccctccg taacgatttt cccgcacccc tactgtgtgg cctacacggg aatctgcaac
     7921 ctgaatttcg agcaggcccg gctggagcgc ctgctcgacg ccgcagacgg cgtggaagtg
     7981 atcaacgccg aaaatattca ccgctggaat ttgcgctgca cgctgctggg gctttacctg
     8041 aaaaaagcga tcaccggcgg cagcgacggc cactccctgt atcatatggg caaggtcgta
     8101 acgtatgcct gctgcgaacc cacgggccca gcgatgctgg atgcgataaa aaacggaagc
     8161 gcccgggtga tcggcaagga gatcaacctg tttcgaaaag tggccgccgg cacgtcgaag
     8221 ctgaatgtgc ccgtcgcggc gtatccggag cggctgggga aaaatatccg ctacagttac
     8281 cggctgattc acgtcaaatc catcctgatc cggaggcagc tccaactgcg catctaccgc
     8341 aggagaaaca ggctgcatcc gctgaggtaa aggaggcgct tcccgatgtt caacaaactg
     8401 ctggcgggtt tccctctgtt gatctttttt ctggttctgg gcgcggtgac ctggcttctg
     8461 ggcgagatcc tgaagccgtt tttctttgca atcttctggg cggttttgct ggcggccatt
     8521 tttgaacccg tcaacgagtg gctgcggcgg aagtttaaaa atccgaatgt ctcggcgggg
     8581 ctggcgctta cagcggtctt tcttacaatg attcttccgg tcgggctgat tttcagcctg
     8641 ctcgtcgggc agtcccttga tctctaccag tccattaatc aaaacggcgg ttcgtggatg
     8701 caaaccatca ccggcttcct ccagtccgtc agcagccatc ccctgctggc gcgttttcaa
     8761 ctggatcatg cattcatcac cgataaatcc gtcgaacttt taaaaacctt taccgaattt
     8821 ctggttaaga atctgtcggc gtttacgcag aatacggtgg ttttcctggt caaattcgcc
     8881 gtggcgctct actgcctgtt ttattttttc cgcgacggga agcagttgtt tggaagcatt
     8941 gtacgccatc tgccggtgaa tgaaacccat atccatacct ttacgtccga atttctcgtc
     9001 acgtcgaagg ccgcgctgaa attcactttt gtcatcggcg gcattcaggg gcttttgggc
     9061 gggctggttt tttatttcac gggaatcagc aatccgctga tctggggcgt gctgatggtc
     9121 ggattgtcca ttgtgcccgc catcggcaac gcgatcatct gggcgcccgc gggcatcatc
     9181 atgctcgcga tggggcatgt ctggcaggga atcgccatcc ttttcttcgg cgtcgtggtg
     9241 atcagttccg tggacaacct gctccgtccg attttgctgg gcaaggacat ccagatgcat
     9301 ccgctgctga tttttctgtc caccctgggc ggcatcgctg ttttcggatt ttccggcttc
     9361 gtcctggggc cggtggtcgc ctcctttttc ctggccagct ggaaattgtt tctggatctc
     9421 tcttccgagg ccaaaacgga ttcttccggc ggagagactg gctgatcttt ccgtgccgta
     9481 ctgcgttgcg gacattcgcg gtttacaacc gttgtgtttc ggctcccgga cgaatcagga
     9541 accgccacgg cgctttccgg tccttctcac gggcgtaatc gatgccgatt ctgggcgtag
     9601 ataaaatgtc gcgggtttgc acactttgat cgttctccac ccataaacgc tctcccaggg
     9661 tcaggtccca gccgttcagg gatttgtcga ttgccagcgc ctgacacagt ttggcgggtc
     9721 cgttggtcag ttcttttccg tgtttttttc tcctggactg catttcttcg aggccggaaa
     9781 ggggcagaag agcccgcacg agcaccgcgc agggatttcc ctcctcctcg gtcaccaggt
     9841 tcagcaggta atgcatcccg taggtgaaat agacataggc cacgcccggc cggccgaaca
     9901 tgacggtgtt gcggggcgtt ttcccccggt gggcgtggca ggcgctgtcc gagcttccgc
     9961 aataggcttc cgtctcgatg atgacgcccg acagttttgc gccgtccgtt tgacgcacca
    10021 gttttttccc caggagttcc cgcgcgaccg taattgtcgg ccgcgcataa aaggctcttt
    10081 ccagaatgct catgccgctc acctgcccgg tggttttcaa tttcaacttg acttccgcct
    10141 gcgcttcgtt ttaagtcctc tctaccatga agcgccggaa tgtcaacccc ggcgcccggc
    10201 cggcgcggtt ttgtccgtgc gccggcctgt tcgcagtgca tacacatcaa catccggaat
    10261 cagaagcggg gacgcagagg ttctgatcgc atatctgtaa ggagaaagta tgagtgaagc
    10321 caaaggaacg gcggctgttt ttccggggca gggttcccag cggcagggca tgggaaaaga
    10381 tttttatgat gccattcccg tgtgccgggc aacgtttgaa gaagcgtccg acgccctggg
    10441 gctggatgtg gcggccgttt gttttgagga ggatgcccgc ctgcatctga ccgaatacac
    10501 gcagccctgc ctggtgacga cggaaacggc catgttccgg gggctggccg aacgatacgg
    10561 atggaaagcc gactgcttcg gcggtcattc gctgggtgaa ttttcggcgc tggtggccgc
    10621 gggcgtcatg ccctttgccg atgcggtccg gatcgtccgg acgcgcggcc ggctgatgca
    10681 ggaagcgatt ccggtaggag tgggcgcgat ggccgcaatc atcgccgaag gcatcgacgc
    10741 ggggacgctt tcgaccgctc tggcggattt gccggttgac atcgccaaca tcaattccgc
    10801 caatcaggtg gtggtgagcg gcgaggcggc ggccctgccc gaggcgggaa accgatgcaa
    10861 ggatctgttt tccggcccca aatcctgccg ggtggttccg ctcaacgtca gcgcgccttt
    10921 tcacagccgc ttcatgaaaa aaatcgaaga gccgtttcgg gaagccctca aaatgatcag
    10981 cggctcgtgg gatgttcaaa acgctcccgc cgtcacgtcc aattaccggg gagggtttca
    11041 tccggctgat gcggacggga ttgcggacaa tctggtccac cagctttcct cttccgtttt
    11101 atggcgcgac aatatgcagg cgcttgccgg ccgcgccggc gcgatttatg aaatcgggcc
    11161 cggacgcccg ctgcgcgact tctttaaaac catcggtgtg acctgcgaat ccgttataaa
    11221 cctggcggcc gcggaaaaaa tcttcggcgg tgtttcgtga cggaccggtt ttgcgcattc
    11281 tggtccagga aggacgaccc atggatttag gcttcagtga aaatcagttg ctgtatgtgg
    11341 acaccgtgcg caggctggtg aaaaatgaaa tcagtccccg cattctggag atggaaaagg
    11401 aacatggctt tccgtgggcg atcatctcca aagcctggga aatgggcctc gtcaacctgt
    11461 gcattccgga gtccatcaag ggattcgatg tggacgtgtt ttcttccgcg ttgatcattc
    11521 aggagctgtc ttacggcgac acgggcatcg ccacgtcggc catgtgcaac gatctggcca
    11581 acgtcgtgat cagccggcac ggaacggacc agcagaagga gaggtttttg cgcccctttg
    11641 tggaaaaacc gctcctggcg tctttctgtc tgacggagcc gggcgccggg tccgacaact
    11701 cgctcatgac cagcttcatt gcgccgcgcg acgacggttc ctgggtgctc aacggctcca
    11761 aatgcttcat caccaatgcg tcctacgctt cgcaatttac cgttttgtgt aaaaacggaa
    11821 aaccttccag caacttcatg gcctgcgtgg tggttccggc ggaatccgtg attgccgcgg
    11881 accttcccga tgtcggatat tccacccggg agatccagct gtccaccggc ggcagaatcg
    11941 tgatcggcaa acccgaggac aagctcggcc agcgcctgtc caatacggcc agcgtcactt
    12001 tcgaagacgt gatcgttgag cccgatcaga tcatcggcaa ccgccgcttc ggcttcaaat
    12061 acatcatgga cgtgcttgac tttgcccgcc cgatggtcgc ggccatcggc ctggggctgg
    12121 ccaaaagggc gctggatgtt acgatggact acacgcgcca gcgcaagcag ttcggccagc
    12181 gcatctgcga tctgccggtc gcccgcgaca cgcttgtcgc catgtggaaa aaagtggagc
    12241 tggccgagct gtcgctgtgg aaagcctgct ggaaaatcca aaagaacgac aaggacgcgg
    12301 gcatctttgc gtccctggcc aaaaatacct gcgcggaagc ggctcttttc tgcgccaacg
    12361 aagggctgca tctgcacggc ggctacgggt ttacggcgga ttatgaaatt tccaagctgg
    12421 cgcgcgacgc ccatatcatc gacatttatg aaggcgtgcg cgaggtgcag aatatgatta
    12481 tcggaagaga gatcgtgtaa ggggggaaac atgaagattc aggatgtgat cgcactggtg
    12541 accggcggcg cctcgggact ggggggcgcg acggtgctgg aattggccgc gcgcggcgca
    12601 aaagtcgtca tcgtcgatct ggacgtggaa aagggcgaaa aactggccgg ggaagccggg
    12661 gaaaatgtta tgtttgtccg ctgcgacgtg accggcgaag actccgtccg ccaagccatt
    12721 caacaatccc tggatcgctt cgggagcatc acggcggccg tcaactgcgc gggcgtgccg
    12781 gacgcggcca aagtgatggg caaaaaaggt ccgctgcctc tttccctgtt caatcgcgtg
    12841 atttccatca atctggtggg aaccctgaat gtgattcgcc tggccgtcga acagatggtg
    12901 aacaatgccc ccaatgagga aggcgaaagg ggggtgatcg tcaatacggc atccattgcc
    12961 gcgtttgacg gacagatcgg ccaggcggct tacgccgcgt ccaaaggcgg cattgtcggg
    13021 atgacgctgc cgatcgcccg ggaatgcgcc gattacggca ttcgcgtggc cacgatcgcg
    13081 ccggggcttt ttgaaacgcc gctcatggcc ggattgccgc ccgccgtcaa ggaagacatg
    13141 gccaaagggg ttccctttcc ccggcgtctg ggaaggcccg cggagtttgc ccggatggcg
    13201 gttcacatcc tcgagaatcc gatgctgaac ggatgctgca tccgcctgga cggagcgctg
    13261 cgcatgccgc cccggtagcc cctgaatact atccggtgat ttccttgcgc agctggatcg
    13321 ggtccgtctg ctttttccgc atgcggatgt tcagcatttc caccgccacg gaaaacgcca
    13381 tcgcaaagta aatgtagcct ttcggcacat gcacgtcaaa gccttcgacc atcagcgtga
    13441 cgcccaccag gattaaaaag gacagcgcca gaatcttgat ggtcggatgg gcgtccacaa
    13501 attcggcaat cggtttggcg gcaaccagca tgatgcccac ggcgacgatg atggcgattg
    13561 ccatgatcga gacgtgccgg gccaggccga cggccgtgat gacggaatcc agcgagaaaa
    13621 cgacgtccaa aagcgcgatc tgaatcagga tcggcgccag agacgcggtt ttcagcttcg
    13681 gtttggcctc ctcgacccct tccaggctgc tgtggatttc atgtgtggct ttggccagca
    13741 gaaacagccc gccgccgatt aaaataatgt cccggccgga aatgtcctgc ccgaaaaggg
    13801 taaaccaggg ctgggtcaga ttcgccaccc acacgatgga aaacagcagc gcgagccggg
    13861 tgaccattgc cagcatcaat cccagatttc gcgcgaagct tcttttctgg gcgggcagcc
    13921 tccccaccag aatggaaatg aaaataatgt tgtcgatgcc caggacgatc tccagcgccg
    13981 tcagggtggc cagggcaatc caggcctccg gactggcgat ccatgcaaac atgtctttct
    14041 cctgatattt ttaatattcg attcccgccc gccccttgat gccggcggca taatggtgct
    14101 tgatgtcctt gatttcactg accgtgtcgg ccagccggat gacttctttg gctgcgtacc
    14161 ggcccgtcag aatcaaatcg agccccggcg gcttgtcggt gatgaggcgg atcagatccg
    14221 caagttcaat cagcttgaag tccaccgcca cgttgatttc gtcgagaatg accatgtcgt
    14281 accggccgga cgcaatcgct tttttggccg attcaaagcc cttccgcgcc agttcgatat
    14341 ccaccggggc cgggctgccg cgcatgacga agctgtccag gcctgagcgg cgaatggtca
    14401 gggaaggcag atatcggctc ccggccgtat actcgccgta gttgcggcct ttcataaact
    14461 gcatgatgta aaccctgtat ccctggccga cggcgcgcag cgcctgtccg aacgccgagg
    14521 tggttttgcc ttttccgttg ccggtgatca cgatgaccag tcctcgctcc gcgggggcgg
    14581 aagtcgtcgt tggtttctta cgggcggcga ctttttttac agatgcatgg cttttgtcgg
    14641 tttttttcat tcaaaaacat cctcctgaat atagtggcca ttgcaggtgc cggattggat
    14701 atttacggcc tgttgcccaa ataccctttt cgcttgcctg cgagcgtttt gtataaatct
    14761 aaggctcgcg aaaggctttg acaaaactcg ccttcggctc aaacagttgt caaaggctga
    14821 tctttcgctt cgccaagatt
//
LOCUS       DAOSRM010000054        14522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4233_length_14522_cov_111.484966, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000054 DAOSRM010000000
VERSION     DAOSRM010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 14522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14522
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4233_length_14522_cov_111.484966"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..769)
                     /locus_tag="PLA58_06500"
     CDS             complement(122..769)
                     /locus_tag="PLA58_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOR62482.1"
                     /translation="MKRAVVIGLGIFGYHLARTLFENGFEVVAIDKNKDVIQKIRDYS
                     TKAILTDGTDQEIWDDIGLQEDDIAIISFGEDLAAATLITLHLKQKKVKNIIVKAPNE
                     DHKLILEKVGATDVIIPEMDIARKLAKSMISPNVMDYIPLSEDYMIFELAPPNSFLGK
                     TLAELQLRNRFNIEVIAVRDVVADKVNMVPHAGFVVKDGEVLVVIGKEKDISKIK"
     gene            complement(789..2159)
                     /locus_tag="PLA58_06505"
     CDS             complement(789..2159)
                     /locus_tag="PLA58_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOR62483.1"
                     /translation="MKLYLILKAFFSRRLTPARIFAISFVLLILLGTLALWFPFAASR
                     QSLSFTDALFASTSAVCVTGLATIDIGKDLSFIGQLITLFLFQVGGLGIITFSIVLFA
                     LMGRGISFKGREIAQSAFMHTPSRDFYLIIKSVLRYTLIIEGIGTALLFIRFLFDFPV
                     EKALYHALYNAVSAFNNCGYSLFPDSMTRYQGDILVNVTIMLLIILGGIGFIVLHELT
                     DKFRGRRKRLSLHTKIVLITTSALILIGAVCIFFMEKHNVLVGVSLKTAVLISFFQSV
                     VARTAGFSTVDIGVLTNSSILLLLFLMFVGASPGSTGGGVKNTSFAILCLLIVNRMRG
                     NENVNIANRTIPSELVDRTISIIFASVIIIGVITGALLLFSTSEGATLAKRTEFVEYI
                     FETFSAFGTVGLSMGVTPRLNDMQKYMIILMMFIGRVGPLTLAFAWYSHRKKSITYAE
                     ETIMVG"
     gene            complement(2261..2911)
                     /locus_tag="PLA58_06510"
     CDS             complement(2261..2911)
                     /locus_tag="PLA58_06510"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR62484.1"
                     /translation="MLKKYKFILADKLQNIFYYLIRAYSRTFRLRVENEKPWLDHLRG
                     GGRILLCCWHQQFFSAVRYFKNYAPYHPALMISQSKDGDVIARIAEKTGWHTVRGSSS
                     KDGGAALKEMISHLSRYGFAGHILDGPRGPAGVVKAGVIRLAQVKGIFVIPIYATADR
                     AWYFKSWDRFMLPKPFARVTLRYGEMLDLTLKAGEEDFERQRARLQEIMLPGLHTF"
     gene            complement(2953..3372)
                     /locus_tag="PLA58_06515"
     CDS             complement(2953..3372)
                     /locus_tag="PLA58_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR62485.1"
                     /translation="MAKIAVLLVDDEEDFRFIFIRQIKRFFPDSEFEFFEAGDGEKAL
                     ELLRTGIKPSIIILDYTMPKMSGTELLRTIDREHGNLLHVPRVVLSGYIGEDAIREVE
                     SLRCDYIEKSMNTKVFYQQFCRYLTEKLGLSCKPTTP"
     gene            3681..4706
                     /gene="rfaE1"
                     /locus_tag="PLA58_06520"
     CDS             3681..4706
                     /gene="rfaE1"
                     /locus_tag="PLA58_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOR62486.1"
                     /translation="MKSDMGGEHCRQVMRGIARKKALEIIKNYPRSGVLVVGDVMVDH
                     FIWGRVSRISPEAPVPVVDVQKDSVMLGGCANVLNAIYAMGGRVFVAGVIGEDDIGRG
                     LVSQLQERRIDTKGILVEKARPTTLKTRIVAHGQQVVRFDHESRKPIADESAADILDY
                     ARSLRDQIGAIVISDYSKGVVSRNLVEGIKAMAAGSNIFLCVDPKSSDFGIYPGVDVL
                     TPNHHEAQRAAGMEIVNGDDLLKLGETLLNKFDFQALLVTRGEEGMSLFERGRGIRHT
                     HFPAQAKEVYDVTGAGDTVIGVLALSLAARANLKEATALANHAAGIVVGKVGTSTVSR
                     EELIGVL"
     gene            4703..5266
                     /locus_tag="PLA58_06525"
     CDS             4703..5266
                     /locus_tag="PLA58_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOR62487.1"
                     /translation="MSKPTQAAAVKLIFSVFAPAGNLINDAIRRLSVRYGQPDYVSAQ
                     IPFDDTAYYAPEMGQTLVRRFLSMEGLIRPESLPDVKLAANEIENETLLGSRRQVNID
                     PGYLSQAHLILATGKGYTHRPYLRDGIYADLTLMYQAKKFCSLPWTYPDYANEKLWTM
                     FGAIRSRYLLQLKMAGHTSQAVCLRKV"
     gene            5269..6147
                     /locus_tag="PLA58_06530"
     CDS             5269..6147
                     /locus_tag="PLA58_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOR62488.1"
                     /translation="MIKSMTGYGRVETICDGRNIVVEAKSVNHRFLEIALRTPAALYP
                     LEMEYKKKIGEKFRRGRIDVSIRLEGESAESSRVNLNLEIARGYVDVLTRLKNEFDLR
                     ESVSLQNLIGFRDIFTPPAETQMGAAFLNRVEQTLQEALSMLVHMRQDEGIALFSDMQ
                     SRLQAIREILEAVRLRAPQVVLDYQKRLSERVRELTAGMEPDPARLAQEVAILADRCD
                     ITEEIVRLQSHIGQFEELLESDEAEGRKIDFLLQEMNREINTIGSKSSDVDISRQVIE
                     VKSELGKLREQAQNIE"
     gene            6152..6760
                     /gene="gmk"
                     /locus_tag="PLA58_06535"
     CDS             6152..6760
                     /gene="gmk"
                     /locus_tag="PLA58_06535"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOR62489.1"
                     /translation="MTEKGLCIVVSAPSGSGKSTICRRLLAACPDMEFSVSFTSRPPR
                     PNEIDGKDYHFVSREDFQRRIERGEFVEWVENYGEFYGTSGRALQDLLRRGKDLLLDI
                     EPRGAKEMKKKFQEGVFVFVLPPSLDELLKRLQKRGHETPEAIQKRFAQAASELEEVL
                     WYDYAVMNEDLDTAVGQLIAIYRAEKCRTSRLLGAINHLFHR"
     gene            6789..7058
                     /gene="rpoZ"
                     /locus_tag="PLA58_06540"
     CDS             6789..7058
                     /gene="rpoZ"
                     /locus_tag="PLA58_06540"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOR62490.1"
                     /translation="MARITIEDSLKVAKTRFGLVLLASKRARQLLKGSKPLVESKNRE
                     IVLALREIAQGKVVYAHPEFLNGTQEDFKPIPDNTEFIGDEEYLE"
     gene            7039..7797
                     /gene="pyrF"
                     /locus_tag="PLA58_06545"
     CDS             7039..7797
                     /gene="pyrF"
                     /locus_tag="PLA58_06545"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOR62491.1"
                     /translation="MKNTSSSRPQDRLIFALDAPGVEEALSWVDRLSGSVGLFKVGKE
                     LFTSAGPAIVERILQKGGRVFLDLKFHDIPNTVARAGEAAVALNVDMFNVHASGGSRM
                     IRETVQAAAACAEKRGAGCPIVLAVTVLTSLNDQDLAEIGFEKTADELALHLAKMARE
                     AGASGVVASPRDILSLRRTLGEDFLIVTPGIRASDEKVADDQKRTLSAFEAISAGADY
                     IVVGRPIRQAQDPLEACRRIIREIADGLEARNRA"
     gene            7827..8582
                     /gene="rlmB"
                     /locus_tag="PLA58_06550"
     CDS             7827..8582
                     /gene="rlmB"
                     /locus_tag="PLA58_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOR62492.1"
                     /translation="MEAVYGINPIKVLLSRDTTPLKKILIAEGRSGPPLQDILASARQ
                     KNIPVEWVPRQRLDGLTDQADHQGIAGLCDAFAYADLEDVLKNRNPRLSQDLVLILDG
                     ILDPQNLGSVIRSAYCLGANGVVIPTDRSASVTPAVMKASAGSAQHLPVARVTNLSRT
                     IDDLKNRGFWIYGADARGGKNLREQDFGTPVGLVLGSESRGLRPLIRKKCDFFISIPM
                     AGNFDSLNVAVAAGIIQYEMLLQRLAKPAGPRP"
     gene            8702..9910
                     /locus_tag="PLA58_06555"
     CDS             8702..9910
                     /locus_tag="PLA58_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOR62493.1"
                     /translation="MPVFLWEGTTKKGEVKKGEMEAPDEPAVRTALRRQGFKSIAVKQ
                     KPKDLLENIPFLQGGVDEKNVVVFCRIFSTMIDAGLPLIQCLSILADQEENKAFAKIL
                     RTIKEDIEGGTSLTDALKKHPKVFDDLFVNLIAAGEAGGILDVVLARLSAYLENAMKL
                     KAAVKSAMTYPITVLCIAAGVVTLLLLKVIPTFQKMFADMGGELPGPTQVVVGMSEFM
                     QNYWWVLGVIVAAMVIGLKMFYKTEKGHWTIDALLLKAPLIGNVLRKVAVAKFTRTLA
                     TMMSSGVPILEGLNIVSKTAGNVVIEAAVVKTRQAISEGQSIAEPLSASGVFPPMVVQ
                     MIAVGEATGALDIMLNKIADFYDDEVDAAVKGMTAMIEPIMMVFLGGVCGGMIIAMYL
                     PIFKMASVVG"
     gene            complement(9917..9993)
                     /locus_tag="PLA58_06560"
     tRNA            complement(9917..9993)
                     /locus_tag="PLA58_06560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9957..9959),aa:Pro,seq:cgg)
     gene            10092..10499
                     /locus_tag="PLA58_06565"
     CDS             10092..10499
                     /locus_tag="PLA58_06565"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HOR62494.1"
                     /translation="MIIYDIKCENGHTFEGWFKDRQAWIEQNAGRLVCCPVCNSSSVE
                     IAPSSLTIMGKDSKPPRNKDVSRGQVLQSLRRYLENNFEDVGNKFAEVALKIHAGDEE
                     RRNIKGTTTPREETVLKEEGVSFIKISLPKMDS"
     gene            10596..12911
                     /gene="cysS"
                     /locus_tag="PLA58_06570"
     CDS             10596..12911
                     /gene="cysS"
                     /locus_tag="PLA58_06570"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOR62495.1"
                     /translation="MMNVYKSIMDRIGNTPLVEISKLNPNKKVTILAKLESANPGGSI
                     KDRTALSMIENAEKRGDLKPGRIILEATSGNTGIGLAMVAAAKGYKLCLTMSEAASEE
                     RKKILRAMGAELIFTPAAQGTDGAIEVAYRMLRENPEKYFGTDQFNNPDNVRAHYFGT
                     AQEIWDQTQGRIAAVVVTLGTTGTAMGISQRLKELDCRIQIIGVEPYLQHKIQGLKNM
                     RESYRPGIYDKKRLDEKVNILDEDAFEMSRRLAREEGILAGMSSGAAMFVAAQKARQM
                     EEGTIVVIFPDSGERYLSTELFAVKEELATVSFYNAIKRRKTLFRPLNPKEVRLRTCG
                     PTVHDAPHLGNYRRLVVSDLLCRYLTFKGYAVKHVVDIVDCSDKSLKGSEKAGMNLAD
                     YSDKYLRIFLEDVRFLQIRPDNIYVRASEDVDAALKIVEKLVDRGYAYEKLHSVYFDI
                     SKLGDYGSLSNIDPAKTRTGRSVDLDDYEKDSPADFALLKRAGLGELKRGIYSKTKWG
                     NVRPGWHLECAAISRKYLGAAYDIHVSGADETFPHCENLIAINKACCGHSGAKYWIGA
                     ELILVDGRKMSRSLNNIVTMDDLRQSGHGGRDVRFFLLGMNYRKPISYSVSALQMARN
                     TIKKMDTFISRLQTIRNAAPNFPEVDQLIYDLNHAFERALDDDLNISGALAALFEFIG
                     AVNAPLAGGKISGEDARKILEALQKINDVLQVMDFKKPIDDRQIDELIQKREKARQAG
                     LWQEADLLRNRLAGLGVDVLDSRQGTIWRFK"
     gene            12922..13743
                     /gene="amrB"
                     /locus_tag="PLA58_06575"
     CDS             12922..13743
                     /gene="amrB"
                     /locus_tag="PLA58_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOR62496.1"
                     /translation="MDNVRKSVISGSWYPGDPSVLRRDIAGYFRRVPDRELSGEVVGL
                     IAPHAGYIYSGQVAAFAYQLVRGKKYDAVVLIGPSHRVAFSGVSVWGGGAFETPLGRV
                     PVHEKLAEEMKRASGIVVDFPAAHAREHSLEIQLPFLQVALGDFSFVPLVMGDQNAAT
                     CRDLAKAIVQAGKNRRLLIVASSDLSHFHEDIKARALDGVALRHLQDSDAEGLLENLA
                     RDRTEACGGGPMAVAILAAGGLGASHSLVLKYTHSGEVTGDCQSVVGYASAVYYK"
     gene            13759..14307
                     /gene="amrA"
                     /locus_tag="PLA58_06580"
     CDS             13759..14307
                     /gene="amrA"
                     /locus_tag="PLA58_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOR62497.1"
                     /translation="MELTDQEKTTLLEIAKRAIIAKVGNRELPKLSMDLPILKEKRGA
                     FVTLKKRGHLRGCIGYIKAVKPLGETVQEMAVAAAFHDPRFPSVKSEEIRDLRYEISV
                     LSPLRKIQSIEEIEVGKHGLYVVRGYNSGLLLPQVATEYGWDRETFLRETCFKAGLPH
                     KAWKDKETEIYIFSANYFSDKD"
BASE COUNT         3415 a         3654 c         4257 g         3196 t
ORIGIN      
        1 cgcgcgttga gtcccgctta agcgggacga gcgttcattc tccgcggctt gccgcgatat
       61 catgacgttc aattcatgcc tcgacagctt gccgcgaggc ggttgatccg gaatttttcg
      121 cttatttgat tttgctgatg tccttctctt tgccgatgac gaccagaact tctccgtctt
      181 tcacgacgaa tccggcatgc ggcaccatgt tcaccttatc ggcaaccaca tcgcgcacgg
      241 cgatgacttc aatattgaaa cggttgcgca gttgcagctc ggccagggtt tttcccagaa
      301 aactgttggg cggagccagc tcgaaaatca tgtaatcctc ggacagggga atgtagtcca
      361 tcacgttggg agagatcatg ctcttggcca gttttctggc aatgtccatt tccgggatga
      421 tgacatccgt cgcgcctact ttttccagaa tcagcttatg gtcttcgttg ggcgctttca
      481 caatgatgtt ttttactttc ttctgtttga gatgaagcgt aatcagcgtt gcggcagcca
      541 ggtcttcgcc gaaggagata atcgcgatat cgtcctcctg aaggccaatg tcatcccaga
      601 tttcctgatc cgtaccgtcg gtcagaatcg ccttggtgga ataatctcga atcttctgaa
      661 tgacatcttt gtttttatca attgccacga cttcaaagcc gttttcaaac aaagttctcg
      721 caaggtgata accaaaaatg cccaacccga taacaaccgc tcttttcatg gatttcctcc
      781 ttatggaact atccgaccat gatggtttcc tccgcatacg ttatgctctt tttccggtgc
      841 gaataccagg cgaaggccag cgtcaacggg ccgacccgtc caatgaacat catcaggata
      901 atcatatatt tctgcatgtc gttgagtctg ggagtgacgc ccatggacag gccaacggtg
      961 ccgaatgcgg agaatgtttc aaaaatatat tcaacaaatt cggttcgctt ggccagtgtg
     1021 gcgccttcgc tggtgctgaa taaaagaagt gcgccggtaa tgacgccgat aatgatgacc
     1081 gatgcaaaaa tgatggatat cgtgcgatcc accagctcgg acggaatcgt gcgattggcg
     1141 atattgacat tctcgtttcc ccgcatacgg ttgacaatca gcaggcacag gatcgcaaag
     1201 ctggtgtttt tgacgccgcc gcccgttgag ccgggcgatg cgccgacaaa cataaggaat
     1261 aagagaagca aaatgctgga attggtcagg acgccgatat ccacggtact gaagcccgcc
     1321 gttcgggcaa ccaccgactg aaaaaaagaa atcagcacgg ctgttttcag ggaaaccccc
     1381 accagaacat tatgcttctc catgaaaaaa atgcaaacgg ccccgatcag aatcagggcg
     1441 gacgttgtga tgaggacgat ttttgtgtgc agggaaagtc ttttccggcg gccgcggaat
     1501 ttatccgtca gctcgtgcag aacgatgaat ccgatgccgc ccagaatgat taagagcata
     1561 atggtgacgt ttaccaggat gtcaccctga tagcgcgtca tgctgtccgg gaaaagggaa
     1621 tatccgcaat tattgaaggc ggatacggca ttatataagg catgatacaa agccttttcg
     1681 accggaaaat caaaaagaaa gcggatgaac agcagcgccg ttccaattcc ttcaatgatc
     1741 agcgtgtagc gcaaaacgct tttaatgatc agataaaaat cacggcttgg ggtgtgcatg
     1801 aaagcggatt gagcgatttc gcgccctttg aaggaaatcc cgcggcccat cagggcaaac
     1861 agaacaatag aaaaagtaat gatgcccagt ccgcccacct gaaaaagaaa cagcgtgatc
     1921 aattggccga tgaacgagag gtctttgccg atatcaatgg tggccaggcc tgtgacgcag
     1981 actgccgacg tggaagcaaa aagcgcgtcg gtaaagctca agctttgccg gctggcggca
     2041 aacggaaacc acaacgccag cgtgcccagc aaaatgagca ggacaaagct tattgcaaaa
     2101 attcttgccg gtgtgagacg gcgcgagaaa aacgctttaa ggatcagata gagtttcata
     2161 ggcgtccttc atgatgaagg ttgaaaactc gccgggattt ttttcgcttt tctggacggc
     2221 tcaaaatcat aaaaaacgct tcccggctgg aggcgcttcc tcaaaaagta tgcaggcccg
     2281 gcaacataat ttcctggagt cttgcccttt ggcgttcaaa atcctcttcg cctgccttca
     2341 acgtgagatc cagcatctcc ccatacctta aggttacccg cgcaaagggt ttgggcagca
     2401 taaagcgatc ccagcttttg aaataccatg cccggtcggc ggtagcgtat atgggaatca
     2461 caaaaattcc cttcacctgg gccaggcgaa tcacgccggc tttgaccact cccgccggcc
     2521 ctcgtggccc gtccaggatg tgaccggcaa aaccgtaacg cgacaggtga gaaatcattt
     2581 cctttaatgc cgcccccccg tctttggatg aagagccgcg cacggtgtgc cagccggttt
     2641 tctcggcaat gcgggcgatg acatcgccgt ctttgctctg gctgatcatc agcgccggat
     2701 gatagggggc ataattcttg aaatagcgga cggctgaaaa aaactgctga tgccagcagc
     2761 acagcagtat ccttccgccg ccccgcaggt gatcgagcca gggtttttca ttttccacgc
     2821 gaagccggaa cgtccggctg tatgcgcgaa tcagataata aaaaatattc tgaagcttgt
     2881 ccgccaggat aaatttgtat tttttcagca ttcaggcgtc ctttccacga cgggattcga
     2941 cggcgcaatt cttcacgggg tcgtcggttt gcaggaaagc cccagttttt ccgtaagata
     3001 ccggcagaac tgctgataaa agactttcgt gttcatgctc ttttcaatat aatcgcatcg
     3061 caggctctcc acttcccgaa tggcgtcctc tccgatatat ccgctcaaca cgacgcgggg
     3121 aacatggagc aggttgccat gttcacggtc tatggttctg agcagctcgg ttccgctcat
     3181 tttaggcatc gtgtagtcca gaataatgat ggaaggtttg atgcctgtcc ggaggagttc
     3241 cagagccttt tcgccgtccc cggcttcaaa aaactcaaat tccgaatcag gaaaaaatcg
     3301 tttgatctgc cggatgaaaa taaagcggaa atcttcttcg tcgtccacca ggaggaccgc
     3361 tatctttgcc ataaacgtcc tttacggttt ctttgttttc ttacgaccgg gcatggatca
     3421 gccgatgggg gcgggtccct cccgtctttg aatgccgcca tcaacagtcc atgaataggt
     3481 tccaatatct tgcatatttc ttgaaattcc gcaacgaagt agtttcttcg gggcagccgg
     3541 cgcgtcctga agaaactaat gacgaaaatc attgacaaat ccggttcccg gtaatagcat
     3601 tccgtcactt ttttcacaaa gggtgttgca tcctgaggaa gttctttaat gagtggtgat
     3661 agtaccctta cggaaaatcg atgaagagcg acatgggcgg ggagcattgc aggcaggtga
     3721 tgagaggaat cgcaagaaag aaggcattgg aaatcattaa gaattatccg cgctccggtg
     3781 tgctggtcgt cggggacgtc atggtggatc atttcatctg gggcagggta tcgcggatat
     3841 ctcccgaggc gccggtgccg gtggtcgatg ttcagaagga ttccgtgatg ctgggcggct
     3901 gcgccaatgt tctcaatgcg atctatgcga tgggcggcag agtttttgtg gccggcgtga
     3961 tcggtgaaga cgatatcggc agaggactgg tcagtcaatt acaggagcgc cggattgaca
     4021 cgaagggcat tctggtggag aaggcaaggc cgaccaccct caaaacaagg atcgtggctc
     4081 acggacagca ggtggtccgc ttcgatcacg agagccgcaa gccgatcgcc gacgaaagcg
     4141 cggcggatat tctcgactat gccaggtccc tgcgcgatca gatcggagca attgtgattt
     4201 ccgattacag caagggtgtg gtttcccgga acctggtgga aggcatcaag gccatggccg
     4261 ccggttccaa tatttttctg tgcgtggatc cgaagtcgag cgatttcggc atctatccgg
     4321 gcgtcgatgt gcttacgccc aaccatcatg aggcgcagcg cgccgccggc atggaaatcg
     4381 tcaacgggga tgacctgctg aaactggggg agacgctcct gaacaaattt gattttcagg
     4441 cgcttctggt aacgcgtggt gaagagggaa tgagtctttt tgaacggggc cgcgggatcc
     4501 gccacaccca ctttcccgct caggccaagg aagtctatga tgttacgggg gccggcgata
     4561 cggtcatcgg tgttctcgcg ctttccctgg ccgcgcgggc gaatctgaag gaggccaccg
     4621 cactggccaa ccacgcggcg ggcattgtcg tgggcaaagt cggaacgtcc acggtgtcgc
     4681 gggaagaatt gatcggcgtg ttatgagcaa accgacgcag gccgcggcgg tcaagctgat
     4741 cttcagcgtt tttgcgccgg cgggaaattt gattaacgac gcgatccgcc ggttgtccgt
     4801 ccgatatggc cagccggact atgtcagcgc gcagatccct tttgacgata cggcgtatta
     4861 tgcgccggaa atggggcaga cgctggtccg gcgttttctg tcgatggagg ggttgatccg
     4921 gccggagtcg ctgccggacg ttaaactggc cgcgaatgaa atcgagaacg agacgctttt
     4981 ggggagccgc cggcaggtga atattgatcc cggctacctg tcgcaggctc atctgattct
     5041 ggccacgggc aaaggctaca cgcaccggcc ctatttgcgg gacgggattt acgcggattt
     5101 gaccctgatg tatcaggcca aaaagttttg ttccctgccc tggacgtatc cggattatgc
     5161 aaacgagaag ctgtggacga tgttcggggc gatccggtcg cgctatctgt tgcagctgaa
     5221 aatggccggg cacacgtcgc aggccgtttg cttacgaaag gtttgaaaat gattaaaagc
     5281 atgaccggtt acggccgggt ggagactatc tgcgacggac ggaacattgt ggtggaagcc
     5341 aaatccgtga atcaccggtt tctggaaatc gctctgcgga cgcccgcggc gctctatccg
     5401 ttggagatgg agtacaaaaa gaaaatcgga gagaaattca ggcgcggccg cattgacgtt
     5461 tccatccgcc tggaaggcga gagcgccgaa tcctcgaggg tgaatctgaa tctggagatt
     5521 gcgcgcggct acgtggatgt gctgacccgt ctgaaaaatg aattcgattt gcgggaatcc
     5581 gtcagccttc agaacctgat cggctttcgg gatattttta cgccgcccgc ggaaacgcag
     5641 atgggagccg cttttttgaa ccgggtggag cagaccctgc aggaagcttt gtcgatgctg
     5701 gtccacatgc ggcaggatga aggaattgcc ctgttttcgg atatgcaaag caggcttcag
     5761 gccatccggg aaatcctgga ggccgttcgc ctgcgggcgc cgcaggtcgt gctggactac
     5821 cagaagcgtc tttccgagcg cgtccgggag ctgacggcgg gaatggagcc ggatccggcc
     5881 cgcctggcgc aggaagtggc catcctggcg gatcgatgcg acatcacgga ggaaattgtc
     5941 cgcttgcaga gccacatcgg tcagtttgag gaactgctcg aaagcgatga ggcggaaggc
     6001 cgcaaaatcg actttcttct tcaggaaatg aaccgtgaga tcaatacgat cggctccaaa
     6061 agcagcgacg tggatatttc ccgccaggtg atcgaagtga aaagcgaact gggcaaattg
     6121 cgcgaacagg cgcagaatat cgagtaggcg tatgacggaa aagggacttt gcatcgttgt
     6181 gtccgctccc tccgggtccg ggaaaagcac catttgtcgc cggctgctgg cggcctgtcc
     6241 ggacatggaa ttttccgttt cctttacgtc gcggccgccg cgtcccaacg agatcgacgg
     6301 aaaagattat cattttgttt cccgtgaaga ttttcagcgc cggatcgaac ggggcgagtt
     6361 tgtggaatgg gtggaaaatt acggcgaatt ttacggaaca tccggtcggg ctctgcagga
     6421 tttgctccgt cgcggaaaag atctgctgct cgacattgaa ccgcgcgggg caaaggaaat
     6481 gaagaaaaaa tttcaggagg gcgtttttgt ttttgtgctg cccccctctt tggacgagct
     6541 cctgaaacga ttgcaaaagc gggggcacga aactccggag gcgattcaaa aacgttttgc
     6601 ccaggccgca agtgaacttg aagaagtttt gtggtatgat tacgccgtca tgaacgaaga
     6661 tctggacacg gccgtggggc agttgatcgc gatttaccgg gcggaaaaat gcagaacgag
     6721 ccgtttgcta ggcgcaatta atcatttatt tcatcgataa aaaaagaggt gtaggaggtg
     6781 ctcgattcat ggcaagaatt accatagaag attcgctcaa ggtggcgaaa acccggttcg
     6841 gcctggtgtt gctggcatcc aagcgcgcgc ggcagctgct gaaagggtcc aagcccctgg
     6901 tggaaagcaa aaaccgagag attgttcttg cgcttcggga aattgcccag gggaaggtcg
     6961 tttatgccca tccggaattt ttaaacggga cgcaggaaga tttcaaaccg ataccggaca
     7021 acacagagtt catcggcgat gaagaatacc tcgagtagcc ggccccagga ccggctgatc
     7081 tttgcgctgg atgcgcccgg cgttgaagag gcgctgtcct gggtggaccg tctttccggc
     7141 agcgtcggcc tgttcaaagt cgggaaggaa ttgtttacat ccgccggacc ggccatcgtg
     7201 gagcgcattt tgcaaaaagg cggacgggtg tttctggatc tcaagtttca tgatattccc
     7261 aacacggtcg cccgcgccgg tgaagccgct gtggcgttga acgtggatat gttcaatgtg
     7321 cacgcttccg gcggaagcag gatgatccgg gaaaccgtcc aggccgcggc cgcctgcgcc
     7381 gaaaagcggg gcgccggatg tcccatcgtg ctggccgtga cggtgctgac cagtctgaac
     7441 gatcaggatc tggcggaaat cggtttcgag aaaacggcgg acgagctggc gctgcatctg
     7501 gcgaaaatgg cccgtgaggc cggagcgtcg ggtgtggtgg cttcgcccag ggatatcctt
     7561 tcgttgaggc ggacgctggg ggaagatttt ctgattgtga cgcccggcat ccgcgcatcc
     7621 gatgaaaaag tggcggatga tcaaaaaaga acgctcagcg cttttgaagc gatttcggca
     7681 ggcgccgatt acattgtggt gggcagaccc atccggcagg ctcaggatcc gctggaggcc
     7741 tgccgccgga tcattcggga gattgccgac ggcctggaag cccgaaaccg cgcatgatcc
     7801 tatctcttac gcacgggagg cggagcatgg aggcagttta cggcatcaac cccatcaagg
     7861 ttctcctttc ccgggacacg acccctttga aaaaaatttt gattgccgaa ggccggagcg
     7921 gtccgccgct tcaggacatt ctggcctcgg cccggcaaaa gaacatcccc gttgaatggg
     7981 tgccccgcca gcgtctggat ggtttgacgg atcaggccga tcatcagggc attgccggtt
     8041 tgtgcgatgc tttcgcctac gccgatctgg aagacgtgct gaaaaaccgc aacccgcgcc
     8101 tgtctcagga tctcgttttg attcttgacg gcattctgga tccgcaaaat ctgggatcgg
     8161 tgatccgcag cgcctattgt ctgggcgcca acggcgtcgt gattccgacc gatcggtcgg
     8221 cgtccgtcac gcctgcggtc atgaaggcct cggccggcag cgctcagcac ctgccggttg
     8281 cccgcgtgac gaatttgtcc cggacgatcg atgatttgaa aaacagaggc ttctggattt
     8341 acggggcgga tgcccgcggg gggaaaaacc tcagagagca ggattttggc acgccggtgg
     8401 gcctggttct gggaagcgaa tccagaggat tgcggccgct gatccggaag aaatgcgatt
     8461 tttttatttc cattccgatg gcggggaatt ttgactcttt gaatgttgct gtggcggcgg
     8521 ggatcattca atacgaaatg ctgcttcagc ggctggcgaa gccggccggt ccccggccgt
     8581 gaaggccgaa ggcgcgggaa gggatgattt ctgaatttaa ccggttgaaa ctgcacggaa
     8641 gatggtttat gatgcagtca taaaaaaacg attcctcatc aagaagaaag gatgttgacg
     8701 catgccggtt ttcctttggg aaggaacgac aaaaaagggc gaggtcaaaa aaggcgaaat
     8761 ggaggctccc gatgagccgg ccgtccgcac ggcgttgagg cgccagggat tcaaatccat
     8821 cgcggtcaag caaaagccga aagatcttct ggaaaacatt ccctttcttc agggaggcgt
     8881 ggatgaaaaa aacgtggtgg tgttctgccg gattttttcc acgatgatcg acgccggttt
     8941 gccgctgatt cagtgcctga gtatcctggc cgaccaggaa gaaaacaagg cgtttgccaa
     9001 aatcctccgg acgatcaagg aggatatcga agggggcact tctctgacgg atgcgctgaa
     9061 aaaacatccg aaggtatttg acgacctgtt tgtcaacctg atcgccgccg gcgaagcggg
     9121 cggcattctg gacgtcgttc tggcgcgcct ttccgcgtat ctggaaaacg cgatgaaact
     9181 caaggcagcc gtcaaaagcg ccatgaccta ccccattacc gtgttgtgca ttgccgccgg
     9241 cgtggtgacg ctgctgcttt tgaaggtcat tcccaccttt cagaagatgt ttgccgacat
     9301 gggcggcgag cttcccggtc cgacgcaggt agtcgtgggg atgagcgaat tcatgcagaa
     9361 ttactggtgg gtgctcgggg tgatcgttgc cgcgatggtg atcggcctga aaatgtttta
     9421 taaaaccgaa aaaggccact ggacgattga tgcgctgctg ctgaaggcgc cgctgatcgg
     9481 caatgtgctc aggaaagtcg ccgtcgccaa atttacgcga acgctggcca cgatgatgag
     9541 ctccggcgtg ccgattctgg agggattgaa tatcgtcagc aaaaccgccg ggaatgtcgt
     9601 catcgaggcg gccgtcgtga aaacgcgcca ggccatcagc gaaggccagt ccattgccga
     9661 gccgctgtct gcctcaggcg tgtttccgcc gatggtggtg cagatgatag cggtggggga
     9721 agccaccggc gcgctggata ttatgctcaa caaaatcgcc gatttttatg atgacgaagt
     9781 ggatgcggcg gtcaagggga tgacggcgat gatcgaaccg atcatgatgg tttttctggg
     9841 aggcgtttgc ggcggcatga ttatcgccat gtatctgccg atcttcaaga tggcatccgt
     9901 cgtgggctga aaaaaatggt cgggatggcg cgatttgaac gcgcgactcc tgcgtcccga
     9961 acgcagtgcc ctaccaagct gggccacatc ccgacacggg aagcgacgtt ataaaattta
    10021 tcgcgaaatg tccacatgtt tttatttcca acggattttt agccgttttt aacaaggaaa
    10081 gcgcccgagc ggtgattatt tacgacatca aatgtgaaaa cggacacacc tttgaaggct
    10141 ggtttaaaga ccgccaggcc tggatagaac agaatgccgg gcgtctggtc tgctgtcccg
    10201 tctgcaacag ttccagcgtg gaaatcgcgc cttcttccct gacgattatg gggaaggatt
    10261 ccaagccgcc ccggaacaag gacgtttccc gggggcaggt gcttcagtcg ttgcgccgct
    10321 accttgaaaa caactttgaa gatgtcggaa acaaatttgc cgaagttgcg ctgaagatcc
    10381 acgcgggcga tgaagagagg cgcaatatca aaggaacgac gaccccccgg gaagaaacgg
    10441 tcttgaaaga agaaggggtt tcgtttataa aaatttctct gcccaagatg gacagctgat
    10501 ccgttatatt tcctccggga tgactttttc aggcgcctgc gcaatccttg tttcaaaaag
    10561 gaatgatgaa ggattgacga acggagcgta gggtgatgat gaacgtgtat aaaagcatta
    10621 tggatcgcat cggcaacacg ccgctggtgg aaatctcgaa gctgaatccg aataaaaagg
    10681 tcacgattct ggccaagctg gagtccgcga atcccggggg atccattaaa gaccgaacgg
    10741 cgctcagcat gatcgaaaac gccgaaaaaa ggggcgacct gaaacccggc aggatcattc
    10801 tggaggcgac cagcggcaat accggcatcg gactggccat ggtggccgcg gcgaaaggct
    10861 acaagctgtg cctgaccatg tccgaagcgg ccagcgaaga gcgcaaaaaa attctgcggg
    10921 cgatgggcgc ggagctgatc tttacccccg cggcgcaggg gacggacggc gccatcgagg
    10981 tcgcctaccg gatgcttcgg gaaaacccgg aaaagtattt cggcaccgat caattcaaca
    11041 accccgataa cgtccgggcc cattatttcg ggacggcgca ggaaatctgg gatcagaccc
    11101 aaggccggat cgccgccgtg gtggtcacgc tcggaaccac cggcaccgcg atgggcattt
    11161 cgcagcggct caaggaactc gattgccgca ttcagatcat cggcgtcgag ccttacctgc
    11221 agcataagat ccagggcctc aaaaacatgc gcgagtccta ccgccccggg atctacgaca
    11281 agaagcgtct ggatgaaaag gtgaatatcc tggacgaaga cgcttttgaa atgtcccgcc
    11341 ggctggcccg ggaagaaggc atcctcgccg gcatgagctc cggcgcggcc atgtttgtcg
    11401 cggcccagaa ggcccgtcag atggaagaag gcacgatcgt ggtcattttc ccggacagcg
    11461 gagagcgcta cctgagcacg gaattgttcg cggtcaaaga ggagctggcg acggtcagct
    11521 tttacaacgc gatcaagcgc cggaaaacgc ttttccgtcc gctgaacccg aaggaggtgc
    11581 ggctgcgcac ctgcggcccg accgttcatg acgctcctca tctgggaaat taccggcggc
    11641 tggtggtgtc cgacctgctg tgccgctatc tgaccttcaa gggctacgcc gtcaaacatg
    11701 tcgtcgatat cgtcgattgc agcgacaagt ctctcaaggg gtcggaaaag gcgggcatga
    11761 atctcgcgga ttattccgat aaatatttgc ggatatttct ggaggatgtc cggtttttgc
    11821 agatccgtcc ggacaatatc tacgtaagag cctccgaaga tgttgacgcc gcgctcaaga
    11881 tcgttgaaaa gctggtcgat cgcggatacg cctatgagaa gctgcactcc gtttatttcg
    11941 acatctccaa actgggagac tacggcagtc tgtccaatat cgatccggcc aagacccgga
    12001 ccggccggtc ggtcgatctg gatgattacg aaaaggactc tccggcggac ttcgcgcttt
    12061 tgaaaagggc cggtctggga gagctgaaaa gggggattta ttccaagacg aagtggggca
    12121 atgtccgtcc gggctggcat ctggaatgcg cggccatttc ccggaaatat ctgggcgcgg
    12181 cctatgacat ccatgtcagc ggagcggatg aaacctttcc ccactgcgag aatctcatcg
    12241 ccatcaacaa ggcctgctgc ggacacagcg gcgcaaaata ctggatcggg gccgaattga
    12301 tcctggtgga cggccggaag atgtcccggt cattgaataa tatcgtaacg atggatgacc
    12361 tcagacaaag cgggcacggc ggccgggatg tccgtttttt tctgctgggc atgaattacc
    12421 gcaagccgat cagctactcg gtaagcgctc tgcaaatggc cagaaacacc atcaagaaaa
    12481 tggacacctt catttcccga ttgcagacga ttagaaatgc cgccccgaat tttccggagg
    12541 tggatcaact gatttatgac cttaaccacg cctttgagcg ggccctggac gatgacctca
    12601 acatttccgg cgcactggcg gcgctttttg aatttatcgg cgccgtcaat gcgcccctgg
    12661 ccgggggaaa aatcagcggg gaggacgccc gcaagattct ggaggcgctt caaaaaatca
    12721 atgacgtctt gcaagtcatg gatttcaaaa aaccaatcga cgatcggcag atcgacgagc
    12781 tgattcaaaa aagggagaaa gccagacagg ccggcctctg gcaggaggcg gatttattga
    12841 gaaaccggct ggccgggctc ggcgttgatg ttctggacag ccggcaggga accatctggc
    12901 gtttcaagta gaggcaggcg aatggacaac gtgcgaaaat ccgtcatttc cggctcctgg
    12961 tatcccggcg atccgtccgt tttgaggcgg gacattgccg gatatttccg cagggttccc
    13021 gaccgggagc tttccggaga ggtggtcggc ctcatcgcgc ctcatgccgg ctacatttac
    13081 tccggtcagg tggcggcctt cgcttatcag cttgtccggg gcaaaaaata cgacgcagtg
    13141 gtgttgatcg ggccctccca ccgggtggct ttttcggggg tttccgtctg gggcggcggg
    13201 gcttttgaaa cccccctggg ccgggtgccg gtccatgaaa aactggcgga agaaatgaag
    13261 cgcgcgagcg ggattgttgt cgattttccg gcggctcacg cccgcgagca ttccctggaa
    13321 attcagcttc ctttcctgca ggttgctctg ggcgatttct cctttgttcc gctggtgatg
    13381 ggggatcaga acgccgcaac ctgccgggat ttggcgaagg cgatcgtgca ggcgggcaaa
    13441 aaccggcgcc tgctgattgt cgccagcagc gatctgtcgc attttcatga ggatataaaa
    13501 gcaagagcgc tggatggcgt ggcgcttagg cacctgcagg acagcgatgc tgaaggtctt
    13561 ctggagaatc tggcgcgcga ccgcacggag gcctgcggcg gtggtccaat ggcggtggcc
    13621 atactggccg ccgggggtct gggcgcttct cattcgctcg ttttaaagta cacccattcc
    13681 ggagaagtga ccggggattg tcaatccgtg gtcggctatg cttcagccgt ttattacaaa
    13741 taggaaaaag aggttcatat ggaactgaca gatcaggaaa aaacgacgct tttggaaatt
    13801 gccaagaggg cgattatcgc caaggtcggg aatcgtgaat tgcccaagct atcgatggat
    13861 ttgcccatcc tgaaggaaaa aagaggcgcg tttgtcacct tgaaaaaaag ggggcatctg
    13921 cgcggctgca tcggatacat caaggcggtc aaaccgctgg gggaaacggt gcaggaaatg
    13981 gcggtcgccg cggcttttca tgatccgcgt tttccgtccg taaaaagcga agaaatacgg
    14041 gacttaaggt atgaaatatc agtgttgagc cctctgcgga agattcaaag cattgaggaa
    14101 attgaggtgg gaaagcacgg tctttacgtc gtcagaggct ataattccgg cttgcttctg
    14161 ccgcaggtgg cgaccgagta cggctgggac cgggaaacgt ttcttcggga gacgtgcttc
    14221 aaagcgggtt tgccccataa ggcatggaaa gacaaggaaa cggaaattta tatcttctcc
    14281 gcaaattatt tcagcgacaa ggattaaccc cttttctgcg ccggaagatt tttcaaataa
    14341 tttaaaaatt tacttgacaa aaaccattat aaaaattagt gtcctcaatt cgcacgaagc
    14401 agtgtagtcg tttttttgcg ttcaaaataa ttacggtttc gtgcaaaagc gaaatgattt
    14461 aaatggtttg ctggcgtagc tcaatcggta gagcagctga tttgtaatca gcaggttgcg
    14521 gg
//
LOCUS       DAOSRM010000055        14347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4295_length_14347_cov_102.304436, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000055 DAOSRM010000000
VERSION     DAOSRM010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 14347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14347
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4295_length_14347_cov_102.304436"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLA58_06585"
     CDS             <1..326
                     /locus_tag="PLA58_06585"
                     /inference="COORDINATES: protein motif:HMM:NF018531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HOR62498.1"
                     /translation="KAVEQARKEKVIGHSLDARISIALPDKMKDLFAAHLEDLRALLI
                     VSQLQLVEESAIANPYKSEEIKGLAVGVEKARGAKCERCWIYEESVGANADHPTVCGR
                     CLSNL"
     gene            528..1025
                     /gene="lspA"
                     /locus_tag="PLA58_06590"
     CDS             528..1025
                     /gene="lspA"
                     /locus_tag="PLA58_06590"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOR62499.1"
                     /translation="MKKNLLIFVLGAAVIIALDQITKSAVMARFSLHETLEIIPGLFN
                     LVYVMNPGAAFGFLADASPGFRYAFFTGVTILAAGLIVYYLVKSNPRNLMLAGSLTLI
                     FGGAVGNLIDRIRFGAVVDFLDVYLGASHWPAFNVADSAITVGAILMIWEMLRNRKSQ
                     PTSPT"
     gene            1128..1403
                     /locus_tag="PLA58_06595"
     CDS             1128..1403
                     /locus_tag="PLA58_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62500.1"
                     /translation="MNTALKQANFQIPEDLLNELRTTVGKSELSKFVSQALKRELQSL
                     RQQTAIDETFGIWKNQEHPELAKGINDFVRTLRKSTRGKRADAANRR"
     gene            1384..1767
                     /locus_tag="PLA58_06600"
     CDS             1384..1767
                     /locus_tag="PLA58_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOR62501.1"
                     /translation="MRQIVVDTDILILHLRGYGDARKALSEAARNSLLCCSAITVGEI
                     YAGMKETEREPTEKLLDSLLVIDVDKNIAALAGYYRRTIKSHQLELDDCLIAATCAIH
                     KATLITGNVKHYPMKDFEKKSIKII"
     gene            complement(1817..2611)
                     /locus_tag="PLA58_06605"
     CDS             complement(1817..2611)
                     /locus_tag="PLA58_06605"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOR62502.1"
                     /translation="MTTTIFYYTGTGNSLWTARRLASALGETQCVSLKRCTEEKIVCG
                     AERIGLVFPVHIWGVPPPVVEFVGRLNVDPAQYLFALAVNAGQPAATLIQLQSILREK
                     QLCLSSGFSIDLPSNYIPWGGAPASGKQQKKFDAVLAKLNRIAAVVRGRDERPPEKGP
                     FWQNWMLSALYRISLPHVAGMDKPFFADEKCSACGICEKICPARNIEMAEEKPVWKHR
                     CEQCFACIQWCPEEAIQYGKGTKNKKRYRHPEISLKDMLASVSPER"
     gene            2811..4307
                     /gene="glpK"
                     /locus_tag="PLA58_06610"
     CDS             2811..4307
                     /gene="glpK"
                     /locus_tag="PLA58_06610"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOR62503.1"
                     /translation="MKKSYILALDQGTTSSRAVIYDRDGVVVKIAQNEFSQIYPQAGW
                     VEHDPMEIWGSQRGAASEVMAATGISPEEIAAIGITNQRETTIVWDKATGRPVYNAIV
                     WQCRRTASICDELKAAGLEDTIRENTGLVVDAYFSGTKLKWILDHVEGARDRARKGEL
                     LFGNVDTWLIWNLTRGKVHVTDYSNASRTMLYNIRELKWDSMLLEKLDIPASMLPEVK
                     PSSAVYGVTDARVLGGEIPIAGDAGDQQAALFGQACFTPGMVKNTYGTGCFMLMNTGE
                     KPALSEKGLLTTIAWGVGGKVEYALEGSIFVAGAAIQWLRDSLRVIYDARDSEYFATQ
                     VPDTHGVYLVPAFVGLGAPYWDMYARGAIVGLTRGANRNHIIRATLESIAYQTRDVLE
                     LMRSECGIDLCELRVDGGACANNFLMQFQSDILDVPVERPEIIETTALGAAYLAGLAT
                     GFWNDQATISEKRKVNRRFEPAMDAKQRQTLYDRWKRAVERARGWEEE"
     gene            4429..5940
                     /locus_tag="PLA58_06615"
     CDS             4429..5940
                     /locus_tag="PLA58_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR62504.1"
                     /translation="MIKTDVIIIGGGATGCGIARDLALRGIPHCLIEKGDFAAGATGA
                     CHGLLHSGGRYAVSDPIAAKECYDENLILRRIGEKCVERTGGLFVRLPGDSKLFRERF
                     LRSCDDIGIPTEILSSTKALELVPNLNPDIEEAIRVPDCSIDPFRLCLLNMKTSQMKG
                     GLIFIRHRVTEIVQSHGRCIGVKATDAATGEVKEIHGNILVNASGAWADLVARLAGCE
                     VPLTLSKGSLVITNHRLTDMVINRLRPSSDGDIIVPNEAVCLAGTTSLSVEDPDALST
                     QSAEVDAIIGEAGRMIPHFHKARIIRAFSGVRPLLKSQKMDSREISRGFQIIDHQNGM
                     YSIVGGKLTTFRLMAEKMVDTIMNAFNLKHDCETADQPLDGQEELSGYPLSRRLADMK
                     NILCECELVSRGRVENIIRETGTRHVGDISHRTRLGMGPCQGGFCTFRALGVMHDMEI
                     LTAQQSVQSLREFLQRRFRGIRYALWGDQLREEQLVEYIYLGILAMEKTRERH"
     gene            5927..7123
                     /gene="glpB"
                     /locus_tag="PLA58_06620"
     CDS             5927..7123
                     /gene="glpB"
                     /locus_tag="PLA58_06620"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpB"
                     /protein_id="HOR62505.1"
                     /translation="MSVIEYDVIVIGAGAAGLTAAAWLAQQGVSVALVTAGEPTACLS
                     TGCIDVCSRDDQPLSGIAGLPDEHPFHLVPEAMLRQSIEDFRQMMADMDMPYEGTLEK
                     NRRILSAIGAFKTTCLVPATMQASPQSDNERIHLITFTGLKDFYPGYLLSRFSNASFS
                     AYDAGVSTTMGIAAHFEDSAFRETFLLWLERQNIREDKIAFPAVLGLESAFSVLKQIE
                     SRLERPVFEIPTLPPSMPGRRLFNGMKDHLRRRGGVIYWNWPVVGVEKSDRQIEAVST
                     GAQGRPNSLNARAFILATGSFVGGGLWAKRDRIVEKVFDLPVFAPENRDDWFDQDYFS
                     VSHGIGQAGIVVDDAFCPQGSPWNNVFVCGSILAHSEALKNGCGHGFAIATGRAAARS
                     CLERLR"
     gene            7120..8304
                     /locus_tag="PLA58_06625"
     CDS             7120..8304
                     /locus_tag="PLA58_06625"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HOR62506.1"
                     /translation="MTFEHCIRCTICVESCPVFRVQPEFPGPKQAGPDAQRFRIGREK
                     SIDDWVLLCSQCKRCEMACPCGVEPAQIILRVQQRYGLEHPHTPAHLLFANNYYLSAL
                     GSMTAPLANRIAAAKIGKKILGQMGIATYIPFPKFSFKTLRGEKKIRSRGNRKIAFFY
                     GCFINFYRPDIGRKIMRMLQSFDINVVLPPQVCCGLPALGNGNLALARTFARKNVASL
                     SNYIDAGYDIVYTCTSCGLCLLQDYMGILEIPEAKKIAENSYDLHEYLLKLIAEREIS
                     PVFREVNRSLAYHIPCHLRALKIGYPAAELMKRIPGLDVAILDDACCGLSGSYGFKTT
                     NEATACELGARAVSLIRKTGAKAIVADCGSCRMQLSGLSGMNAFDPSEILCESLGIRN
                     KK"
     gene            8825..10651
                     /locus_tag="PLA58_06630"
     CDS             8825..10651
                     /locus_tag="PLA58_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR62507.1"
                     /translation="MAIFEYGEYQNTYESPDNLVEIFENSVSKYANNLFIGEKDSSGV
                     YQWVTYAQIAARVDNLRGGLASLGVQAGDTVGIIANNRKEWVIGEMATQGLRAAFVPM
                     YEKELVTMWKYIIKDAAVKVLLVSKPEILEKVKYFPAEIPTLQHIYLIEGTGDKTMAA
                     LEKKGAEKPVPSQKPKYSDVAVLIYTSGTTGEPKGVLLSHGNLAENVKGALAWFLNLT
                     EEGRCISMLPWAHSFGITADLHAFMLRGGSIGIMGSVETLIDDLPKIQPSFMTTVPRV
                     FNKVYNGVWAKMREEGGLKLKLFEMALDAAKQKRETGKAGLKFKLLDKIVLNKIRERF
                     GGRLVNAVTGSAPMNVEIAKFFIDVGIPTYDAYGLSETSPAITINSPLMGNRLGSVGK
                     PINKTKVVIDRSRTGDNSDDGEIICFGPQCMIGYHNKPEKTKEVIVEMNGMRGVRTGD
                     RGRLDDDGFLFITGRFKEEYKLANGKYIHPDAVELEMKVLPWILNAMIYGAGQEYNVG
                     LIVPDMKLLGQMAEKLNLSVKPESLFDPGNPAGRNFKELLTAEVQNHLRKTIGSYEIP
                     QKFAFILEDFTLESGMLTQTMKLKRQAVLDKYGDLLNSLYKE"
     gene            complement(10731..11429)
                     /locus_tag="PLA58_06635"
     CDS             complement(10731..11429)
                     /locus_tag="PLA58_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62508.1"
                     /translation="MMIRKEIIERNPLLNLGFDREYILSDGGLGAVLAHAGVGKTALL
                     VQTALHAMMHEQPVLHVSLNDAVSKVDLWYRELFNDMASKYDSAEITQYWENMLHYRF
                     IMTFRVEGFSVPKLEERLADLMQQNIFKPHTVILDGLKFDESGRGLLVELKEMAGKYA
                     MRIWCTVHTHRHEPPKEDGLPLSFLHVADLFDVLVQLSSRGPEVYIKALKGQSPDSRQ
                     DALLLDPATMLIKS"
     gene            11448..>11627
                     /locus_tag="PLA58_06640"
     CDS             11448..>11627
                     /locus_tag="PLA58_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62509.1"
                     /translation="MNDIVFPSDRSFYCHRSYFTAFIRPSAFGLQPFYSLQSSAFSSR
                     RRFLVPGRFQGYFLRN"
     assembly_gap    11628..11727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11729..13816)
                     /gene="fusA"
                     /locus_tag="PLA58_06645"
     CDS             complement(11729..13816)
                     /gene="fusA"
                     /locus_tag="PLA58_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR62510.1"
                     /translation="MKFDDQKIRNIGISAHIDSGKTTLSERILFYTKRIHAIHDVKGK
                     DGVGATMDSMDLEKERGITIASAATYCEWKGHEINIIDTPGHVDFTIEVERSLRVLDG
                     AILVLCSVGGVQSQSITVDQQMKRYKVPCIAFINKCDRSGANPFRVIEQLRTKLGHNA
                     VAMQIPVGLETELEGVVDLVAMKAVYFDGESGEEVRVEDIPPHLRAEAAAKREALVDA
                     ASLFSDELTEAILEEKEISPELLYAALRRGTLKRELTPVYMGSAYKNKAIQPLLDGVT
                     YLLPAPSEVENVALDMNQNEEPVVLTGDPEKPIIALAFKLEDGQYGQLTYIRVYEGTI
                     EKGATIVNVRNGKKVKVGRVVRMHADQMEDVDSLRAGYIGALFGIECSSGDTFVSPGI
                     NMTMTSMYVPKPVISLAIVPKDNKAQMAMSKALNRFSKEDPTFKTHVDPETNETIIEG
                     MGELHLDIYVERMKREYNAEVTTGNPRVAYRETITQRADFNYTHKKQTGGSGQFGRIA
                     GYIEPISDAEFVFENKIFGGAIPTQYIPACEKGFRQSMAKGPKLEFPITGVKVVIDDG
                     SSHPVDSSDMAFQAAARGAFRDAYLRAKPVVHEPIMKVVVESPTEFQGSVMGLLNQRR
                     GMIIGSQDEGVMCVIESQVPLGEMFGFSTVLRSATQGKAQFTMEFALYRQVPQSIAEK
                     IALEAARNKKTAA"
BASE COUNT         3231 a         3968 c         3884 g         3164 t
ORIGIN      
        1 ccaaagccgt cgaacaggcg cgcaaggaaa aagtgatcgg ccattcgctg gatgcccgga
       61 tcagcatcgc tctgccggat aaaatgaaag atctgtttgc cgcgcacctg gaagacctgc
      121 gcgcgctgtt gattgtttcg caactgcagt tggtcgaaga atccgccatt gccaaccctt
      181 acaaaagcga ggaaatcaaa ggactggccg tcggcgtgga aaaggcccgc ggcgccaaat
      241 gtgaacgctg ctggatttat gaagaatcgg tgggcgcaaa cgcggatcat cccacggtct
      301 gcgggcgctg tctgtcgaat ttgtagagaa tgggtccttg gcaagaggca attggcaaga
      361 ggccataggc aatgggcgat gggaaaaagt gaatgtaatg cggaccttca ggtccgcctg
      421 acgcggatct aaagacccgc gctgcattcc tgtaaaaagt gacgagcagg gaacggtcgc
      481 gaccgttccc tgcagaagtt atggtgcatc gttgcagagg agtttttttg aagaaaaatc
      541 ttttaatttt cgttctgggc gcggcggtca tcatcgcgct ggaccagatc accaaatcgg
      601 ccgtgatggc gcgtttttca cttcatgaaa cgctggaaat cattcccgga ttgttcaacc
      661 tggtctatgt catgaatccc ggcgcggcct tcggttttct ggcggacgcc tccccgggat
      721 tccgttatgc ttttttcacc ggcgtgacca ttctggccgc cggcttaatt gtctattatc
      781 tggtgaaaag caatccgcga aacctgatgc tggccggctc cctcacgctg attttcggcg
      841 gagcggtcgg caatctgatt gaccggatac ggttcggcgc cgtcgtggac tttctggatg
      901 tgtatctcgg cgcctcgcac tggcctgcct tcaatgtggc ggactccgcc atcaccgtcg
      961 gcgctattct gatgatctgg gaaatgctcc gcaaccggaa gagccagcca acatcgccaa
     1021 cctgatcgac aacgatctgc gagctacagt aatatttcac tgttgacttt tatcgttata
     1081 tttataaaaa tatttacaaa gagctataga atatggaggc aaagctcatg aatactgcat
     1141 tgaagcaggc caattttcag attcctgaag acctgctgaa cgaactgcgg acgactgtcg
     1201 gaaaaagcga attaagcaag ttcgtcagcc aggctctgaa gcgcgaactc cagagtctgc
     1261 ggcagcagac agccattgat gagaccttcg gcatatggaa aaaccaggaa caccctgaat
     1321 tggcaaaggg aataaatgat tttgtgcgta cattgagaaa atcgacgcgg ggaaagaggg
     1381 ccgatgcggc aaatcgtcgt tgacacggac atcctgatcc ttcatctgcg aggctacgga
     1441 gatgcccgca aggcgctcag cgaggccgcc cggaattcat tattatgttg ttctgccatc
     1501 accgtcggcg agatatatgc cggcatgaag gaaacagagc gagagccaac tgaaaaatta
     1561 ctggacagcc ttctggtgat tgacgtggac aaaaatattg cagctctggc gggatattac
     1621 cgtcgcacca ttaaaagtca tcaattggaa ctggacgact gcctgattgc cgccacatgc
     1681 gcgatccaca aggccactct catcacgggc aatgtcaaac attatccgat gaaagatttt
     1741 gaaaaaaagt ccatcaaaat aatatagctc cgtcataagg cttttttata taaagagaat
     1801 ttgacatgtt tttgaattac ctctcggggg agacggaagc cagcatgtct ttcagtgaaa
     1861 tttccggatg acggtagcgc tttttattct tcgtgccttt gccgtactgg atcgcctcct
     1921 ccgggcacca ctggatgcag gcaaagcatt gttcacagcg gtgcttccag accggtttct
     1981 cttcagccat ttcaatatta cgcgccggac agattttttc acaaatcccg caggcgctgc
     2041 atttttcatc ggcaaagaaa ggtttatcca tcccggcgac atgcggcagg gaaatccggt
     2101 aaagcgcaga caacatccaa ttctgccaga agggcccctt ctccggcggc cgctcatccc
     2161 tgccccggac caccgccgcg atccggttca atttagcgag caccgcgtca aatttctttt
     2221 gctgcttgcc ggacgccggg gccccgcccc agggaatata gttgctgggc agatcaatgg
     2281 aaaaaccgga tgagagacaa agctgtttct cccgtaaaat gctctgcaac tggatcagcg
     2341 tggcggcggg ctggccggcg ttgacggcca gcgcaaacag gtattgagcc ggatcaacgt
     2401 tgaggcgccc gacaaactcg acgacgggcg gcggcacgcc ccagatatgc accgggaaaa
     2461 caaggccgat ccgctcggcg ccacagacaa tcttctcctc cgtgcaccgc ttcagggaga
     2521 cgcattgtgt ctctcccaga gcggaagcca gtctgcgggc ggtccacagg gagttgcctg
     2581 tgccggtata gtaaaagatg gttgtggtca tggtcctgaa cttccggtga ttgcgtcttt
     2641 gttttttggc aggctatccc cgccgggctt cttctgtcaa atgtttttat gaagcggtag
     2701 gctccccgaa aggcgccgct gcgctgcctg ccgccttttt tctttggcaa aaacatttgt
     2761 attatgtcat agtgcccgaa aatattgatt catgtgttca ggagtggccg atgaagaaat
     2821 cctacatttt ggcgcttgac cagggcacga ccagttcgcg ggcggtgatt tacgaccggg
     2881 atggcgtcgt ggtaaaaatc gctcaaaacg aattttcaca aatttatccc caggccggct
     2941 gggtggaaca tgacccgatg gaaatctggg gctcgcaacg cggcgcggcc agcgaagtga
     3001 tggccgccac cggcatctcc cccgaagaga tcgcggcgat cggcattacc aatcagcgcg
     3061 aaaccaccat cgtctgggat aaagccaccg gcaggcccgt ctataatgcc atcgtctggc
     3121 agtgccgcag gaccgcgtct atctgcgacg aactgaaggc cgccggcctc gaagacacca
     3181 tccgggaaaa cacggggctg gtggtggacg cgtacttctc gggaacaaag cttaaatgga
     3241 ttctcgatca tgtcgaaggc gcccgcgaca gggcgcggaa aggcgaactg ctcttcggca
     3301 atgtcgatac ctggctgatc tggaacctca cccgcggaaa agtccatgtc acggattaca
     3361 gcaacgcttc ccgcacgatg ctgtacaata tccgggaact gaaatgggat tcgatgcttc
     3421 tcgagaagct ggatattccc gcctccatgc tgccggaggt caagccctct tccgccgtgt
     3481 acggcgtcac cgatgcgcgc gttctgggcg gcgaaatccc catcgccgga gacgcgggcg
     3541 atcagcaggc ggcgctgttc ggccaggcct gcttcacgcc cggcatggtc aagaacactt
     3601 acggcaccgg ctgtttcatg ttgatgaata cgggagaaaa gccggccctt tcggaaaagg
     3661 ggttgttgac aaccattgcc tggggagtgg gcggcaaagt ggaatacgcc ctggaaggca
     3721 gtatctttgt ggccggcgcg gcgattcagt ggctgcgcga cagcctgcgc gtcatttacg
     3781 atgccaggga cagcgaatat tttgccactc aagttcccga cacgcatggg gtttatctgg
     3841 tgcccgcctt tgtgggactg ggcgcgccct actgggacat gtacgcccgc ggcgccattg
     3901 tcggactcac gcgcggagcc aaccgcaacc atattatccg cgccacgctg gaatccatcg
     3961 cctaccagac gcgcgacgtt ctggagctga tgcgcagcga atgcggtatt gatttgtgcg
     4021 aacttcgcgt ggacggcggc gcctgcgcca acaacttcct catgcagttc cagtcggaca
     4081 ttcttgacgt cccggtggag cgtccggaaa tcattgaaac cacggccctt ggcgccgcct
     4141 atctggcggg tctggccacg ggattctgga atgaccaggc cacgatttca gagaaacgga
     4201 aagtgaaccg gagatttgaa cccgccatgg acgcaaagca gaggcaaacg ctttatgacc
     4261 gctggaaaag agcggtggaa cgcgccagag gatgggaaga ggagtaagga aaggctgaag
     4321 gctgaaggct gaagactgaa ggctgaaggc tgaagactga aggctgaaga ctgaaggctg
     4381 aaggctgaag accgaaggct gaaggctaaa aggaagattt tattttagat gatcaagacg
     4441 gatgtcatca ttatcggcgg aggcgccacg ggctgcggca ttgcccggga cctggcgctc
     4501 cgcggcatcc ctcactgcct gattgagaaa ggcgatttcg cggcgggagc caccggcgcc
     4561 tgccacgggc tcttgcacag cggcggccgg tacgcggtgt cggatcccat cgcggccaaa
     4621 gaatgctacg atgaaaatct gattctgcgg cgcatcggcg aaaaatgcgt ggagcgcacc
     4681 ggcggattat tcgtgaggct gcccggcgat tccaaactct tccgggaaag atttttgcga
     4741 agctgcgacg acatcggcat ccccaccgaa attctttctt ccaccaaagc gctggaactc
     4801 gtgcccaatt tgaatccgga catcgaggaa gccatccggg ttcccgactg ctcgatcgat
     4861 cctttccgcc tgtgcctgct caacatgaaa acctcgcaga tgaaaggggg cttgattttc
     4921 atccgccacc gggtcacgga aatcgtccaa tcccacggcc gttgcatcgg cgtcaaagcc
     4981 accgacgcgg ccacgggcga agtaaaagaa atccacggca acattctggt caatgcctcg
     5041 ggcgcgtggg ccgatctcgt ggcccggctg gccggctgcg aagtgccgct gacgctctcc
     5101 aaaggcagcc tggtcatcac caatcaccgc ctgaccgaca tggtcatcaa tcgcctgcgg
     5161 ccttcctcgg acggcgacat cattgttccc aacgaagccg tgtgtctcgc cggcaccacg
     5221 tcgctttctg tggaagaccc ggatgcactc tcgacccagt ccgcggaggt ggacgccatc
     5281 atcggcgagg ccggccggat gattcctcat tttcataagg cgcgcattat ccgggcgttt
     5341 tcaggcgtcc gtcccctgct caaatcgcaa aagatggaca gccgggaaat ctcgcgcggc
     5401 tttcagatta tcgaccatca aaacggtatg tacagcatcg tcggcggcaa actcaccacc
     5461 ttccggctga tggcggaaaa aatggtcgat accatcatga acgcctttaa tctgaagcac
     5521 gactgcgaaa cggccgacca gccgctggac ggccaggagg agctcagcgg gtatcccctg
     5581 tcccgccgac tggcggacat gaaaaacatc ctgtgcgaat gtgaactggt aagccgcggc
     5641 cgcgtggaaa atattatccg ggaaaccggc acccgccatg tgggagacat ttcacaccgg
     5701 acgcgcctgg gcatgggccc ctgccaggga ggcttctgca cctttcgcgc gctgggcgtc
     5761 atgcatgaca tggaaattct gacggcccaa cagtccgtgc agtcgcttcg ggaatttcta
     5821 caaaggcgtt ttcgcggaat ccgctacgct ctgtggggag atcagctgcg ggaggagcag
     5881 ctggtcgaat atatttatct gggcattctg gccatggaga aaacccgtga gcgtcattga
     5941 atacgatgtc attgtcatcg gcgcgggcgc ggcgggtctg accgcggccg cctggctcgc
     6001 tcaacagggc gtaagcgtgg cgctggtcac cgccggtgaa ccgaccgcct gcctgtccac
     6061 cggctgcatc gatgtctgct cccgcgacga ccaaccgctt tcgggaatcg ccggtctgcc
     6121 cgatgagcat cccttccatc tggttcccga ggcaatgttg cggcagtcca tcgaagactt
     6181 ccggcagatg atggcggaca tggacatgcc ttatgaggga acgttggaaa aaaaccgccg
     6241 cattctttcg gccatcggcg cgttcaaaac cacctgcctg gtccccgcga cgatgcaggc
     6301 ctccccgcaa agtgacaacg aaaggattca tctcatcacc ttcacgggcc tcaaggattt
     6361 ttatcccggc tatctccttt cccgcttttc gaacgcctcc ttttccgctt atgacgccgg
     6421 ggtttccacc accatgggca tcgccgccca ttttgaggac agcgcctttc gggaaacctt
     6481 tctgctgtgg ctggagcggc agaacatccg cgaagacaag atcgctttcc ccgcggtgct
     6541 gggcctggag tcggcatttt ccgtattgaa gcaaatcgaa tcccggctgg agcgccctgt
     6601 ctttgaaatc cccacccttc cgccgtccat gcccggaaga aggctgttta atggcatgaa
     6661 ggaccatctg cggcgccgtg gaggcgtgat ctactggaac tggccggtcg tcggtgtcga
     6721 aaaatcggac cgccagatcg aagcggtttc gaccggggcg cagggcaggc ccaacagcct
     6781 caatgcccgg gcttttattc tggccaccgg atcctttgtc ggcggagggc tctgggcaaa
     6841 gcgggaccgg atcgtggaaa aggtttttga cctgcccgtt tttgcgccgg aaaaccggga
     6901 tgactggttt gaccaggatt atttttccgt cagccacggc atcggccagg cgggcattgt
     6961 cgtggatgac gcgttttgcc cgcaaggctc tccctggaac aatgttttcg tctgcggttc
     7021 gattctcgcc cattcagagg cgctgaaaaa cggctgcgga cacggctttg cgatcgccac
     7081 gggacgggcc gcggcccggt cctgcctgga gagactgcga tgacgttcga gcattgcatc
     7141 cgctgcacga tttgcgtgga aagctgcccc gtcttccggg tccagccgga atttcccggc
     7201 cccaagcagg cggggcccga cgcccagcgc ttccgcatcg gccgcgaaaa atccatcgac
     7261 gactgggtgc ttctgtgcag ccagtgcaaa cgctgcgaaa tggcctgtcc ctgcggcgtg
     7321 gagccggcgc aaatcattct ccgggtgcag cagcggtacg gcctggaaca cccccacacg
     7381 ccggcgcatc tgctttttgc caacaactac tatctctcgg ccctcggatc aatgacggcg
     7441 ccgctggcca accggatagc cgccgccaaa atcggcaaaa aaatcctcgg acaaatgggc
     7501 atcgcgacgt acatcccctt ccccaaattc agcttcaaaa ccctgcgcgg agaaaagaaa
     7561 atccgcagcc gcggaaaccg gaagatcgct ttcttttacg gctgctttat caacttctac
     7621 cggcccgaca tcggaagaaa gatcatgcgg atgcttcagt cctttgacat caatgtcgtc
     7681 ctgcctcccc aggtgtgttg cgggcttccc gcgctgggaa acggcaacct ggcgctggcg
     7741 cggacctttg cgcgaaaaaa cgtcgcttct ctttccaact atatcgacgc gggatacgac
     7801 atcgtctata cctgcacctc ctgcggtttg tgtctgttgc aggattacat ggggattctg
     7861 gaaattcccg aagcaaaaaa aatcgcggaa aacagctacg acctgcatga atacctgctc
     7921 aagctgatcg cggagcgcga gatctctccc gtcttccggg aagttaaccg ctcgctggcc
     7981 tatcacattc cctgccacct gcgcgcattg aaaatcggct acccggccgc cgagctgatg
     8041 aagcggattc cggggttgga tgtagcgatt ctcgatgacg cctgctgcgg actctccggc
     8101 agctacggct tcaagacaac caatgaagcc acggcttgcg aactgggcgc gcgggccgta
     8161 tcactgatcc gcaaaaccgg ggcaaaggcg attgtcgccg attgcggatc gtgccgtatg
     8221 cagctttccg gcctttcggg gatgaacgcg ttcgatccgt cggagatcct ctgcgaatcg
     8281 ctgggcatcc ggaacaaaaa ataaaatacc tcttgacttg ccgctggttt ttcccttata
     8341 gaggaccatc ggaaagacgg ttatccggcc gctccttcgc tctctcacca cctcagcgct
     8401 ctttccgggc ttcaattttg aatgcaacaa gatgccatca aacgtcgtta gatagagaag
     8461 cgtccagctt aattcgagag ctttgaataa cggccatttt tgtcatttcg accgaaggga
     8521 gaaatcttga acaaacggcc atccttaaga tttctcgtcg ctcacgctcc tcgaaatgac
     8581 attgttcaaa gctctcaatt cattgactaa cgaaacatga cgacctcgta aaaagtcttt
     8641 ttcgcgctct tttcgtcata cctctagcga aggccggtat ccggtaatta caatgagtta
     8701 tggaccccgg cgcctgcccg tactgcgata cggggttcgc cggagtgacg gtttgaattc
     8761 ctttttacga gggcgtcaat tttcagcaac gattaatcaa atttcgaaag gagcgggtaa
     8821 gggcatggcg atttttgaat atggcgagta tcaaaacacg tatgagtccc cggacaatct
     8881 ggtggagatt tttgaaaaca gcgtttccaa atacgccaac aatctgttta ttggagaaaa
     8941 agattcttcc ggggtgtatc agtgggtaac ctacgcgcaa atcgccgccc gcgtcgacaa
     9001 tttgcggggt ggtcttgctt cgctaggggt tcaggccggg gatacggtcg gaatcattgc
     9061 gaacaatcgc aaggaatggg tgatcggaga aatggccacg cagggattgc gcgccgcctt
     9121 cgtgccgatg tacgaaaaag aactggtgac gatgtggaaa tatattatca aggacgccgc
     9181 tgtcaaggtc cttctcgttt ccaagcccga aattctggag aaggtgaaat atttccccgc
     9241 ggaaattcca acactccagc atatctattt gattgaaggc acgggggaca agacgatggc
     9301 cgccctggag aaaaaaggcg ccgaaaaacc cgtcccgtcg cagaagccca aatactccga
     9361 tgttgccgtc ctgatctaca cttcgggaac caccggcgag cccaagggag tgcttctttc
     9421 ccacggcaat ctggcggaaa acgtcaaggg cgcgctcgcc tggtttttaa acctgaccga
     9481 agagggtcga tgcatttcca tgcttccctg ggcgcactct ttcggcatta cggcggacct
     9541 gcacgcgttt atgctgcgcg gcggctccat cggcatcatg ggatcggttg aaaccctgat
     9601 cgatgatctg cccaagattc agccgtcctt catgaccacg gtgccccggg tgttcaacaa
     9661 agtctataac ggcgtctggg ccaaaatgag ggaggaaggc gggctgaagc tgaagctctt
     9721 tgaaatggcg ctggacgcgg caaagcaaaa gcgggaaacg ggcaaggccg ggttgaaatt
     9781 caaacttctg gataagatcg ttttgaataa gatccgggag cggttcggcg gacgcctcgt
     9841 caacgccgta accggcagcg cccccatgaa cgtggaaatc gcgaaatttt tcatcgacgt
     9901 gggaatcccg acttatgacg cttacgggct cagcgaaacg tctccggcca ttaccatcaa
     9961 ctcccccctg atgggcaacc gcctggggtc agtcggaaag ccgatcaaca aaaccaaggt
    10021 ggtaatcgac cggtcgcgga cgggagacaa cagcgacgac ggtgaaatca tctgcttcgg
    10081 cccccagtgc atgatcggct accacaacaa accggaaaag acaaaagaag tgatcgtgga
    10141 aatgaacggg atgcgcggcg tgcgcaccgg agaccggggg cgcctcgacg atgacggatt
    10201 tctgttcatc acgggccgct tcaaggaaga atacaagctg gccaacggca aatacatcca
    10261 cccggacgcc gtcgaactgg aaatgaaggt cctgccctgg atcctgaacg ccatgattta
    10321 cggggcgggt caggaataca acgtgggtct gattgttccc gatatgaagc ttctggggca
    10381 gatggccgag aagctgaatc tgtccgtgaa gccggagagc ctgttcgatc ccggcaatcc
    10441 cgccggccgc aatttcaagg aattgctgac cgccgaggtg cagaatcatt tgagaaaaac
    10501 gatcggatct tatgaaattc cccagaaatt cgccttcatc ctggaggatt tcacgctgga
    10561 aagcggcatg ctcacgcaga ccatgaagct caagcgccag gcggttctgg acaaatacgg
    10621 ggatctgctc aacagtctgt acaaagaata aacggaagtt ctcggttttg acaaaacaaa
    10681 gaaccggaag aaaaaacaat ggaggcggtc cgccggactg cctccattgt ttatgatttg
    10741 atcagcatgg tcgccggatc gagcagcagg gcatcctgcc gggaatcggg cgactggccc
    10801 ttgagggctt tgatatacac ttcgggcccc cgggaagaca actgcaccag cacatcaaaa
    10861 agatccgcca catgaaggaa agaaagaggc aatccgtctt ccttgggcgg ttcatgacga
    10921 tgcgtgtgaa cggtgcacca gatgcgcatg gcgtattttc cggccatttc ctttaattcc
    10981 accagcagcc cccgcccgga ttcatcaaat ttcagaccgt ccagaataac ggtgtgcggc
    11041 ttgaaaatat tctgctgcat cagatcggcc agacgctcct ccagctttgg aacgctgaat
    11101 ccttccaccc ggaacgtcat gataaaccgg taatggagca tgttctccca gtattgcgta
    11161 atttccgcgg aatcatattt tgaggccatg tcgttgaaca attcgcggta ccacaggtcc
    11221 accttgctga ccgcgtcgtt caggctgaca tgcagcacgg gctgttcatg catcatggcg
    11281 tgcagcgccg tctgcacaag cagcgccgtt ttgccgacgc cggcgtgggc cagaaccgcg
    11341 cccaatccgc cgtcggacag gatatattcc ctgtcgaatc ccaggttcaa cagcggattg
    11401 cgttctatga tttctttcct gatcatcatc cgttcctcat acgttaaatg aacgacattg
    11461 tctttccatc tgaccgctct ttctactgcc atcgctctta cttcacagcc tttattcgtc
    11521 cttcagcctt cggtcttcag cctttttata gccttcagtc ttcggccttt tcttcacgcc
    11581 gccgttttct tgttcctggc cgcttccagg gctattttct ccgcaatnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnttc acgccgccgt tttcttgttc ctggccgctt
    11761 ccagggctat tttctccgca atcgactgcg gcacctggcg ataaagggca aactccatgg
    11821 taaactgcgc cttgccctgc gtcgcggatc gaaggacggt tgaaaaaccg aacatttccc
    11881 ccagcggtac ctgagattca atgacgcaca tgacgccttc gtcctgcgaa ccgataatca
    11941 taccgcgtcg ctgattgagg agccccatca ccgacccctg aaattccgtc ggcgattcga
    12001 cgacgacttt catgatcggc tcgtgaacga cgggtttggc gcgcaaatag gcgtcacgaa
    12061 aggcgccgcg tgcggccgcc tgaaacgcca tgtccgatga atccacgggg tgcgacgagc
    12121 cgtcatcgat cacgactttc acgccggtaa tcggaaactc cagcttcggt cctttggcca
    12181 tactctgccg gaagcccttt tcgcacgcgg gaatatactg cgtgggaatg gcgccgccga
    12241 agatcttgtt ttcaaaaaca aattccgcgt cgctgatcgg ttcgatatag ccggcaatgc
    12301 gtccgaactg tccggagccg ccggtctgct ttttatgcgt gtagttgaaa tcggcccgct
    12361 gcgtgatggt ttcacgataa gctacgcggg gattgcccgt ggtaacttcc gcgttgtatt
    12421 cacgcttcat cctctcgaca tagatgtcca gatggagctc gcccattcct tcgatgatgg
    12481 tttcgttggt ttccggatcc acatgggtct tgaacgtagg atcctccttg gaaaagcggt
    12541 tcagggcttt ggacatggcc atttgcgcct tgttgtcttt cggcacgatt gccagcgaaa
    12601 tgaccggttt gggcacgtac atcgaggtca tggtcatatt gatccccggc gacacaaacg
    12661 tgtctcccga cgagcattcg atcccgaaga gggcgccgat gtaaccggcg cgcaaagaat
    12721 caacgtcttc catctgatcg gcgtgcattc gaacgacgcg gccgaccttg accttcttgc
    12781 cgttgcgaac attcacgatg gtcgcgcctt tttcgatggt cccttcataa acgcggatgt
    12841 aggtcaactg tccgtactga ccgtcttcca gcttgaacgc cagcgcgatg atcggttttt
    12901 ccggatcgcc ggtcagaacg accggctctt cattttgatt catgtccaga gcgacattct
    12961 ccacttccga cggggcggga agcagatagg tcaccccgtc aagcaggggt tgtatcgctt
    13021 tgtttttata agcggatccc atgtaaaccg gggtcagctc cctcttcaat gtccctctgc
    13081 gcagagcggc gtaaagaagc tcgggggaaa tttccttctc ctccagaatc gcttccgtca
    13141 attcgtcgga aaataaagat gcggcatcga ccaatgcttc tcttttggct gcggcctcag
    13201 cccgcaaatg cggcggaatg tcctccacgc ggacctcttc gccgctttct ccgtcgaaat
    13261 aaacggcttt catcgccacc agatcgacca cgccttccag ctccgtttca agtccgacgg
    13321 gaatctgcat ggccacggcg ttatgcccca gtttggttcg aagctgttca atgacccgga
    13381 aaggattggc gccgctgcgg tcgcatttat tgataaacgc aatgcagggg actttgtagc
    13441 gcttcatctg ctgatcgacg gtgatggact gggactgaac gcccccgacg gagcacagaa
    13501 ccagaatcgc gccgtccaac acgcgcaggg acctttccac ttcaatcgtg aaatccacgt
    13561 ggccgggagt atcgataatg ttgatttcat ggcccttcca ttcgcaatag gttgccgccg
    13621 acgcgatggt gattcctctt tccttttcca gatccatgga atccatggtc gcgcccacgc
    13681 cgtcttttcc cttcacgtcg tgaatggcat gaattctctt ggtataaaac agaattctct
    13741 cgctgagcgt cgttttaccg gagtcgatat gcgcgctgat cccgatattg cgtattttct
    13801 gatcatcgaa cttcatgatg cttccctttc acaaaatggc cgcccggatt ctacgaaaaa
    13861 aaattccccg cacatggaag agtctctgcc agatggggga tccaccgtaa tcgagcctta
    13921 agaataaggt tggctttcta tagttttagc gcgcgtttgt caagctgttt taatttccgg
    13981 caaattcgct taaggttgaa tatccaggag gactttcaat gcatcactct gccgcccccg
    14041 agcccgggca aaggcttccc gcacaagcgc gagcggatag cgggcggaaa taagccgatc
    14101 cggcggcatg tccggaaagt cacgcatatc ttgcattgcg tcaatgttaa tgctgttgat
    14161 gaactgtctt gtacagtata aatataaaag ttcattaatt aaagaaataa aacgaataaa
    14221 aatattatct ccattgaaaa aataaatact tatactgtat aatttatctt gacatttata
    14281 tttttatact gtataagtta gctgtcgttt caataaaagt gctttttgtt cgatttatca
    14341 ttagtac
//
LOCUS       DAOSRM010000056        14170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4368_length_14170_cov_102.883386, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000056 DAOSRM010000000
VERSION     DAOSRM010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 14170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14170
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4368_length_14170_cov_102.883386"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..373)
                     /gene="rhuM"
                     /locus_tag="PLA58_06650"
     CDS             complement(104..373)
                     /gene="rhuM"
                     /locus_tag="PLA58_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOR62511.1"
                     /translation="MYLDYAEDQARRHKPMHMADWIKKLDAFLRFNERNILTHAGRIS
                     QDMAEEHATTEFEKFEVKRLQREAQEPTSDFDHFVEKTKKIKKNL"
     gene            complement(376..1140)
                     /gene="rhuM"
                     /locus_tag="PLA58_06655"
     CDS             complement(376..1140)
                     /gene="rhuM"
                     /locus_tag="PLA58_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOR62512.1"
                     /translation="MANELIPASSEGQLLIYEDGSLRVQVRIDGKTVWLTQRLIAELY
                     QVTVPTVNEHLKGIYGEGELDTAATIRKFRIVQTEGVRMVARMVEHYNLDAILAVGYR
                     VRSARGTAFRKWATARLSELLVKGFTMDDDRLAAGRTLGDNYFDELLERIRAIRASER
                     MFYQKITDIYATSIDYDSGAEITQTFFATVQNKLHWAIHGHTAAEVIYQRADAQKPHM
                     GLTTWKNAPHGLIRKTDVAVAKNYLAKTKSANSTVW"
     assembly_gap    1546..1645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1891..2493)
                     /locus_tag="PLA58_06660"
     CDS             complement(1891..2493)
                     /locus_tag="PLA58_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HOR62513.1"
                     /translation="MAAKRTFLFRALLGMTCLCWMAGCAPTHIAMQVESHAAPNFNAS
                     AQKRFSFLPPEGEKSLTQKLPVLIRSEMEKKGFVHDDRNPNILVAVRAGTTTEKRREP
                     VVSRPVPVLMPSREGGSPDTHYVIEGGGYYTVQIRQIRMDVFGAAGQKPGNELWQGEV
                     SSEGDEDIGEIAGCLVSGLLRDYPAGRGKSRMTLKLNECR"
     gene            complement(2495..3832)
                     /locus_tag="PLA58_06665"
     CDS             complement(2495..3832)
                     /locus_tag="PLA58_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOR62514.1"
                     /translation="MAENKTRIQKTADFVRKDIWRIRGTGLPPGKSFFLKLARVLILS
                     IRGFDEDKCQLRASSLTFYSLVSIVPIAAMAFGIAKGFGFQKVLEEQLREKLAGHEDI
                     LANVIQFSDALLANTKGGLIAGVGLVVLFWAVVKMLRQIEESFNDIWGIREKRTIGRM
                     FGDYLSLILICPVILVLSSGVNVFITTQVNLILEKFTVLGSFGPVIFFLLRLLPYTLM
                     WALFTFLYIFMPNTQVKFSVGLIAGIITGTIFQVVEWAYITFQIGVAQYNAIYGSFAA
                     LPLFLAWLQLSWLIVLYGAELSFAYQNVETYEFEPDALAASHRLRTLLSLRITHHLVQ
                     RFVRGEKPATARDISNQLEIPIRFVNEILFNLVKSDILSMARTEGSEEQGYQPARDVS
                     TLTVQYVIGAMDKRGLNQTPFTQSPEFAALSASLETFDQTMARQPENKLLKDL"
     gene            complement(3843..6944)
                     /locus_tag="PLA58_06670"
     CDS             complement(3843..6944)
                     /locus_tag="PLA58_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HOR62515.1"
                     /translation="MIGKELNRTVHYDEGRGSLSLRKGLMFTFTGIVIKEKDGVADFV
                     HIRSAFLHLNILPLLKNQISLREVTLDRPVFSLKRDRTGELNIADLLAKPRKETSLKV
                     RNITIEKGSVIFEDQAAGNEGITTRLDDLDCTVDSPLWGSPFQYQMNATVIEDRNQAA
                     LSLKGTFDPAPPDKPVWASTLNASIRLTGSDLQHYAPYLKNKASIERLGGLLDVDTNF
                     SGTPANFSSKGSVAVKSALLDCSRAFSNPLTPQTVRLDYALSRNTGNLSLDVSRLAID
                     RFEAGGRLNIEDLDKEDFFLTATATTSAFSLKEMRPFIPWKIIPKTVGDFIAAHIRDG
                     NFRLSEGTLKGRKSQIAHIDSPENAGVLSLRADVSEGVFSAQQTAPDFHDIRGTLELQ
                     NRLFRLRGMKGLFGQSPFSLEGSISDFARPGPVEYTADMTVQPTREEVVWLLGKDRFR
                     NFSFNGPSTLDLSGKGTASDFRVHANWDLTKASYAFPDILEKPASKTNNLTAEMIVHA
                     ETLTFSSFRCDLPPVKISGSATYNFSGEIPLSLHAESKTMNLRKAAALFPVLRNMNPS
                     GTASLNVSGKGDLLHPASLQWTGNASLSNVSLTVPALDKPVNGLTGQICFRGDKMETG
                     PLKAKIGESDIQGKFSVDDFRKPVVMVHFSADRLHAQDLGWTSPKDDASFSVVSAEAV
                     WKSGDLQVSRLSFGLGKTFFNASGRIRDFAAPKITAEITSPRIHSDDIALLASLKTSK
                     KDKTAPSGATVDATVRADAAFLEGVEFSALQAVLNYTPGKLNVQSLEADVFDGHIRAK
                     GKATLRPDGRNRYEANLAADGISLAKLQSVMNIGDRPITGSLSVSGDLTASGSQFADL
                     KKTAAGALQIRAEKGVLKKFPVLSKIFSLLNVSQLFRLKLPDMAKDGMPYSAITAHAS
                     VQNGVFSSKDFFIAGDAMEISAAGSVDAFRKEVDCIAGIHPLQTLDLIVSKMPVVGWL
                     ILDKNGNVLTIYVKINGDWKDPNVKPMPVRSIGKETLGVFRRLFQLPEKLITDTGEVI
                     LGN"
     gene            complement(7118..8131)
                     /locus_tag="PLA58_06675"
     CDS             complement(7118..8131)
                     /locus_tag="PLA58_06675"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOR62516.1"
                     /translation="MPKTGRHNSLIDVEGILVGHFTSRKYASGVTAILSPEGATAGVD
                     VRGSAPGTRETDLLQPMNLVEKVQAVVLTGGSVYGLATADGVVRRLEKKGWGFPLQDG
                     HVAPIVPAAALFDLGRGKQFVPPIGPKWGAAACDAAGSAGFPLGSVGAGTGAMAGGIK
                     GGLGTASEALPSGIIVAAIVAVNSLGSVIDPATGRFWEIRQGMGSEFGALGKRAVKLP
                     PLPVASAGQNTTIGVVATNAVLTKAQAQKIAQMAHDGFARAIRPSHAMFDGDTIFCLG
                     TNKRKLPATRGFFESPAAEMLTEMGRSTADCMTRAITRGILEATSLCGIRAFRDLDAR
                     GNA"
     gene            8328..9800
                     /gene="malQ"
                     /locus_tag="PLA58_06680"
     CDS             8328..9800
                     /gene="malQ"
                     /locus_tag="PLA58_06680"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOR62517.1"
                     /translation="MKRGSGILLAISSLPGRFGIGTLGEGARKFVDWLGQSGQAYWQI
                     LPISPTGFGDSPYQAFSAFAGNPYFIDLEDLHREGLLPRGALDLCESFFASDPDSVDY
                     YAQYLGRPQVLSLAFRHGAGRLAAGLEAFRKRHQSWLGDYALYMALKGEYEMKPFDQW
                     PEALALRYPAALKEAAARLKEQVDYHIFVQYLFFRQWDALRKYAGGRGIRIIGDMPIY
                     MAPDSADAWVEHDILDRERRVAGCPPDYFSATGQLWGNPLYDWEKLKETEYAWWMRRI
                     AHQLSLYDDLRIDHFRGFEAYWAIPAGAKTAESGQWLPGPGIDFFRRLKKAIGDPPLI
                     AEDLGYLTPEVFELLRETGFPGLKVLHFAFTPGGESIYLPHRYEKNCVVYTGTHDNDT
                     TVGWYRGLAKDELAFLDAYLDGVDEERIHWQLIRLAMSSVADVCIFPMQDVLGLPSSA
                     RMNRPQTAQGNWRWRLTADKLDGELSRKLAGLTELFGRQR"
     gene            9805..11706
                     /gene="glgB"
                     /locus_tag="PLA58_06685"
     CDS             9805..11706
                     /gene="glgB"
                     /locus_tag="PLA58_06685"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOR62518.1"
                     /translation="MIKNDIPQDEIYLFNIGEAQQAYATFGCHRVDESDLHRFLVWAP
                     NAREVGVVGDFNGWDASRNRMERLDSGVFAAFVPGLRDGDCYKYAIQGHDGKTVLKAD
                     PFAFHAEVRPATASKVWSLGGYDWRDAEYLKRRHGQNLLNRPVAIYEMHIDSWRKREG
                     YAFANFREIADELSDYLVEMGFTHVEIMPVLEHPLDDSWGYQVTGFYAVTSRYGTPQD
                     FMYFVDVMHAKGIGVIMDWVPAHFPRDEHGLARFDGTCLYEHQNPMQSDHPQWGTLIF
                     NYARPEVASFLVSSAMFFFDVCHIDGIRVDAVTSMLYLDYARNPGEFVPNEDGGNIDR
                     SAVAFLQKLNSVILTRYPGVMTVAEESTAYPMITKPPYDGGLGFLFKWNMGFMHDTLS
                     YMSMDPFFRQHHHSKLTFSMHYAFTENFILPYSHDEVVHGKKSLLNKMVGAYDEKFAS
                     LRLLLGFMYAHPGKKLLFMGGEFGQFIEWDYRKELDWFLLGYERHGQMRRYVQALNEF
                     YTRTPALYAIDDGWEGFAWLNVNNAKQSAVSFLRRGARPEEVCVCAFNFTPVPVERFV
                     IGLPSGGALRDVFSSDDARFGGSGRGAARVIQAVHKGFEGHPYRAAIDLPPLAAVYFE
                     MDSYEKGLK"
     gene            11703..13184
                     /locus_tag="PLA58_06690"
     CDS             11703..13184
                     /locus_tag="PLA58_06690"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOR62519.1"
                     /translation="MNSALQETLAEKKMKAPPPVLMIGAECAPFAKTGGLADVIGTLT
                     REMKRLGFDARLMLPFHRVIKDRYRERVRHLVSFSVDIGGRSQYVGIEQFDGEDIPVY
                     FIDNEHYFGHAIYCGGNFEGEQYAYFTRAAIESLPMIGFEPRILHVNDWHTAMIPMLL
                     KTQYDRLPQGRCRTVLSIHNLGYQGRFHFDFVSHLLGIESRYYHPDNIEYHGDANFLK
                     GGIVYADKLVTVSPTYAREILTAHYGEGLEGILATRADDLTGILNGIDVTAFDPASDP
                     FIPYPYDASSLSQKQKNKKALIAELGLNLSPEAPLIGMVTRLTRQKGIDLVLRVFEEI
                     IAAGAGFALIGTGDHAYEHFFRGAAHRTGGRAVGVTEFNDPLARRIYAGCDFFLMPSQ
                     FEPCGLSQMIALRYGTLPIVRATGGLRDTVFPYNEYTNEGNGFAFENYNAHDMLHTIR
                     YALGVYENKERLASLCVRAMSQDLSFQKSALAYAKVYVDAAGK"
     gene            13288..>14170
                     /locus_tag="PLA58_06695"
     CDS             13288..>14170
                     /locus_tag="PLA58_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOR62520.1"
                     /translation="MKNRIEQIRGIIEGKLKRYYGRALENASDEEMFQVTAMSIRDIM
                     LERGVLANETVEKKGHKRLCYLSAEFLMGRALVNNLINLGLLAEYRAVMADIGYPFEK
                     LEEQEMEAALGNGGLGRLAACFLDSLSTLNLPVTGYGIRYEYGMFRQHIVDGQQTEVP
                     DDWTAKGDIWEVERREEQVEVRFDGTVEEVWTEGGMTCEHRDYQTVLAVPHDMPVIGF
                     DSHTPATLRLWRAECPSGFDLHSFNKGDFVCMLRQKELAESISKVLYPQDNHMTGRML
                     RLRQYYFLASATMQCIVR"
BASE COUNT         2984 a         3752 c         4081 g         3253 t
ORIGIN      
        1 gtgatttact ggcctggaaa atttgaaaag gatgatagcg cattatggtc cgttcctttt
       61 ctctttgttt ttctttttgt tttctttcct tccggcaagg cttttacaaa ttttttttaa
      121 tctttttcgt tttttccaca aagtggtcaa agtcgctcgt cggctcttgt gcttcacgtt
      181 gcaggcgttt gacctcgaat ttttcaaatt ccgtggtggc gtgttcttcg gccatgtcct
      241 gtgaaattct gccggcatgc gtcagaatat tgcgctcgtt aaaccgcaaa aaggcatcga
      301 gttttttgat ccagtcggcc atgtgcatgg gtttatggcg gcgcgcctga tcttcggcat
      361 aatccagata catggtcacc acacggttga gttcgcggat ttcgtcttcg ccagataatt
      421 cttggcgacg gcaacatcgg ttttacgaat aaggccgtga ggcgcgtttt tccatgtcgt
      481 caatcccatg tgcggctttt gcgcgtcagc ccgttgatag atgacttccg ccgcggtgtg
      541 tccatgaatc gcccagtgca gtttgttttg taccgtggca aaaaaggtct gggttatttc
      601 ggcgccggaa tcgtaatcaa tgctggtggc ataaatatcg gtgattttct ggtagaacat
      661 ccgctccgag gcacggatgg cgcgaatgcg ttcgaggagt tcatcgaagt agttatcgcc
      721 aagcgttcgt ccggctgcca gccggtcgtc atccatggta aatcctttga cgaggagttc
      781 tgaaagacgg gcggtggccc attttcgaaa agctgtgccg cgggcggagc ggacccgata
      841 gcccacggca aggatggcgt cgaggttata atgttccacc atgcgggcaa ccatgcggac
      901 gccttcggtt tgaactattc ggaatttccg aatagttgct gctgtatcga gttcgccctc
      961 tccataaata cctttcaaat gttcattcac agtcggaaca gttacctgat aaagttctgc
     1021 gataagtctt tgcgtcagcc agacggtttt cccgtcgatg cgcacctgca cacgcagaga
     1081 gccgtcttca taaatcagca attgcccttc ggaagatgcg ggaataagtt cattcgccat
     1141 agccgatttc cttgaaaata ttgacagcgc gccagagatc tgcctcgaaa ccaagatttg
     1201 caccgccgtt attgtttttg tctttagcca taatttaact caccattaaa aaaggagcga
     1261 tttcgaatgt taaaaacgta attctgaagg aaattctttg ggtcggcggt catcttacta
     1321 ctttgaaacg actttgcaag aaaaaatcag taatatcata aacaatgcaa gctgatcttt
     1381 gcacaacgca taaaaccgct aagtttgtca tacctctatg taaaaaaagc aagtcccttt
     1441 tttacgtagg ggaagtaaca atcgttctgg cgcaggccgg tatccagaca tcgtccccgc
     1501 ggaagcgggg aaccagttcc tttattcctg gattccggat cgcgtnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnntcgtc cccgcggaag cggggaacca gttcctttat
     1681 tcctggattc cggatcgcgt tgcgcttgtc cggaatgacg gttttgatga tttttgtaat
     1741 tgtgcaaagc tctcaagctg attctacacg gctgcactaa gggaaaaaga gggcgggttt
     1801 caggagacgg gctttgtccg cgctacatcc cggtgaagag catgatgatg acggggacgc
     1861 cgatgacgcg gagaattgcg tattttgccg tcatctgcat tcgttaagct tgagggtcat
     1921 gcgggatttg ccccgtccgg ccggatagtc ccggagcaga ccgctgacca gacatccggc
     1981 gatttcgccg atgtcttcat ccccttcgct tgaaacttcg ccctgccaga gttcattgcc
     2041 cggcttttgg cctgccgcgc cgaaaacatc cattctgatt tggcgaatct gaaccgtata
     2101 gtatccgccg ccctcgatca catagtgcgt gtccggtgat ccaccttcgc gcgacggcat
     2161 caagaccggg accgggcggg acacgaccgg ctcccgcctc ttctcagtgg ttgtgcctgc
     2221 cctcacagcg acgaggatat tcggatttct gtcgtcatgg acaaagccct tcttttccat
     2281 ttcgctgcgg atgaggaccg gcagcttttg cgtcagggac ttttctccct caggaggaag
     2341 aaaggaaaat cttttctggg ccgaggcgtt gaagttcggc gctgcatgac tttcgacctg
     2401 catggcgata tgcgttggag cgcagcccgc catccagcac agacaggtca ttcccagcag
     2461 tgcgcgaaac aaaaatgttc tctttgcggc catgctacaa atccttgagc agcttgtttt
     2521 ccggctgacg cgccatcgtt tgatcaaacg tttccaggga cgctgaaagg gcggcgaatt
     2581 cgggggactg cgtaaaaggc gtctggttca gtcctcgttt atccatcgcg ccgatgacat
     2641 actgtacggt cagcgtgctg acgtcgcgcg cgggctgata accctgttcc tcgcttccct
     2701 ccgtccgggc cattgaaaga atatcgcttt ttaccagatt gaaaagaatt tcattgacga
     2761 agcggatggg aatttccagt tgattggaga tgtcgcgggc cgtcgcgggt ttttctcccc
     2821 ggacgaaccg ctggaccagg tgatgggtga tgcgcaggga aagcagcgtc cggaggcggt
     2881 ggctggctgc gagcgcgtcg ggttcgaact cgtaggtttc cacgttctgg taggcaaaag
     2941 acagctccgc cccgtacagc acgatcagcc agctcaactg aagccaggcg agaaacaggg
     3001 gcagggcggc gaagctccca tagatggcat tgtattgggc aacgccgatc tggaacgtga
     3061 tgtaagccca ttcgactacc tggaaaatcg ttccggtgat gattccggcg atcaggccga
     3121 cggaaaattt tacctgagtg ttcggcatga aaatgtagag gaaggtgaaa agcgcccaca
     3181 tgagggtata gggcagcagc ctcagaagaa aaaagatgac ggggccgaag cttcccaaaa
     3241 cggtgaactt ctccagaatc agattcacct gcgtggtaat gaaaacgttt actccgctcg
     3301 acagcacaag gatgaccggg cagatcaaaa taagcgacag ataatcgccg aacattcgtc
     3361 cgatggttcg tttttcccgg atgccccaga tgtcgttgaa agactcttca atctgccgca
     3421 gcattttgac gacggcccag aaaagaacca ccaggccgac gccggcgatc agtccgccct
     3481 tcgtattggc cagaagggcg tcggaaaact ggatcacatt ggccaggatg tcctcgtggc
     3541 cggccagttt ttcgcgcagt tgttcttcca gcactttctg aaatccgaag cccttggcga
     3601 tgccgaaggc catagccgcg atggggacga tcgaaaccag cgaataaaag gtcagggagg
     3661 acgcgcgaag ctggcatttg tcttcgtcga agccccgaat ggaaaggatc aggaccctgg
     3721 ccagtttcag aaagaaagat tttcccggag ggagccccgt gccgcgaatt ctccagatgt
     3781 cttttcggac gaaatccgcc gttttttgaa tccgcgtttt gttttcggcc atgatgtctc
     3841 ctttagtttc ccagaatgac ttcccccgta tccgtgatca gcttttccgg cagttgaaaa
     3901 agtcgccgga agacgcccag cgtttccttt ccaatggatc ttaccggcat gggcttgacg
     3961 ttcgggtctt tccaatcgcc gttaatctta acataaatcg tgagcacatt cccgttttta
     4021 tccagaatca gccagcccac gaccggcatt tttgacacaa tcagatccag cgtttgcagg
     4081 gggtggatgc cggcgatgca gtccacttct tttcggaaag cgtcaacgct gccggccgcc
     4141 gagatttcca ttgcgtcgcc ggcaataaag aaatcttttg aagaaaacac gccgttttga
     4201 acggaggcat gggcggtgat ggcgctgtaa ggcattccgt ccttggccat gtccggcagt
     4261 ttcagccgaa aaagctgaga aacgttcagc agggagaaaa ttttggaaag aacgggaaac
     4321 tttttcagga cacccttttc agcccggatc tgcaaagcgc cggctgccgt tttcttcaga
     4381 tcggcaaatt gacttccgga agcggtcagg tctccggaga cagacagact gccggtgatc
     4441 ggtctgtctc cgatgttcat aacgctctgg agctttgcca ggctgattcc gtcggcggca
     4501 agattcgctt catagcggtt ccgaccgtcc ggacgaaggg tcgctttgcc ttttgcccgg
     4561 atgtgcccgt caaaaacatc cgcttccagg gactggacgt tcagctttcc gggtgtgtag
     4621 ttcagcacgg cctgcagggc gctgaattca acgccttcca gaaaagccgc gtctgcccga
     4681 acggttgcgt cgaccgttgc gccggagggc gcggttttgt cttttttaga cgttttcagg
     4741 gaggccagaa gggcgatgtc atcggaatgg atacgagggg aggttatctc cgccgtaatt
     4801 ttcggcgcag caaaatcccg gatgcgcccc gacgcgttga aaaaagtttt ccccaggccg
     4861 aatgaaagac ggctgacctg gaggtctccg cttttccaga ctgcctccgc gctgacgacg
     4921 gaaaaagacg cgtcgtcttt cgggcttgtc catccgagat cctgtgcgtg gagccgatcg
     4981 gcgctgaagt gaaccatgac gaccggtttg cgaaaatcat caacgctgaa ctttccctga
     5041 atgtcgcttt ctccgatttt tgctttgagc ggacccgttt ccattttgtc gccccggaaa
     5101 cagatctgcc cggtcaagcc gttaaccggc ttgtcgagcg ccgggaccgt caaagaaacg
     5161 ttgctcagcg acgcgttccc cgtccattgc agagaggcgg gatgaaggag gtcgcccttg
     5221 ccggaaacat tgagcgaggc ggtgcccgaa ggattcatat tcctcaggac aggaaacagg
     5281 gctgcggctt tacgaaggtt catggttttc gattcggcat gcagggaaag aggaatctct
     5341 ccggagaagt tataggtcgc cgaaccgctg atcttcacag gtggcagatc acacctgaag
     5401 gaagaaaatg tcagtgtttc cgcatggact atcatttcgg cggtcaggtt gtttgttttg
     5461 gaggcgggtt tttccaggat gtccggaaac gcataggaag ctttggtcag atcccagttg
     5521 gcatggacgc ggaaatcgga ggccgttccc tttcccgaca gatcgagagt ggacggaccg
     5581 ttgaagctga aattacgaaa ccggtctttt cccagaagcc atacgacttc ctcgcgggtt
     5641 ggctggacgg tcatgtctgc cgtgtattca acgggaccgg gcctcgcaaa atcggaaatg
     5701 cttccttcca gtgaaaaggg cgattggccg aaaagccctt tcatgccgcg cagccggaac
     5761 aggcggtttt gaagttccag ggtgccgcgg atgtcatgaa aatccggcgc ggtttgttgc
     5821 gctgaaaaaa cgccttcact gacgtccgcc cggagggaga ggacccctgc attttccgga
     5881 ctgtcgatat gtgcgatctg acttttcctg cctttgagcg ttccttcgct caggcggaaa
     5941 ttgccgtccc gaatgtgcgc ggcaataaaa tctcccaccg tttttggaat aattttccat
     6001 ggaatgaagg gccgcatttc cttcagggaa aaagccgacg ttgtggcggt agccgtcagg
     6061 aagaaatctt ccttgtccaa gtcctcgatg ttcaggcggc cgccggcttc aaagcgatcg
     6121 attgccaggc gtgacacgtc cagcgaaaga tttccagtgt tgcggctcaa agcgtaatcg
     6181 aggcggaccg tctgcggggt gagcggattg gaaaaagccc tggagcagtc gagcagggcg
     6241 cttttcactg cgacgctgcc ctttgatgaa aaatttgccg gggttccgga aaaatttgtg
     6301 tccacatcca gcaggccgcc cagccgttca atggatgcct tattcttcag atagggagcg
     6361 taatgctgaa gatccgatcc tgtaagccgg atggaagcgt tcagggtgct tgcccaaacg
     6421 ggtttatcgg gcggggcggg gtcaaaggtt cctttcaggg aaagagccgc ctggttccgg
     6481 tcttcgatca cggtcgcatt catttgatat tgaaatggac tgccccacaa aggagaatca
     6541 acggtgcagt cgagatcatc cagacgggtg gtgatccctt catttcccgc agcctgatcc
     6601 tcaaatatca ccgatccttt ttcaatggtg atgttgcgga ctttcagcga tgtttccttc
     6661 cggggttttg ccagaagatc ggcaatgttc agttccccgg ttctgtcccg tttcagtgaa
     6721 aagacgggcc gatccagagt aacttcccta agggagattt gatttttcaa gagggggagt
     6781 atgttcaggt gaaggaaagc ggaacggatg tgaacgaagt cggcaacgcc gtctttttcc
     6841 ttgatgacga tgccggtaaa cgtaaacatc agtcctttgc gaagggaaag cgagcccctg
     6901 ccctcgtcgt aatgaacggt gcggttcagt tctttgccga tcattcttgt tagaggttcc
     6961 ctgaaagcat ccggttgaga gagtttccag gaaatgaaga gccaggcaga tgccagaaga
     7021 acggcgaccg cggcagcgcc tatcccaaaa aataaaatga ttttcttttg cgtcttcaaa
     7081 cagaagcctt tttacgatgg tctttgatta tggagcgtta agcattcccg cgcgcgtcca
     7141 gatcccggaa ggccctgatt ccgcaaaggc tggttgcctc caggattccc cgggtgatgg
     7201 cccgggtcat gcaatctgcg gtgctgcgcc ccatttccgt cagcatttcc gccgccggag
     7261 attcgaaaaa tccacgggtt gccggcagtt tccgcttgtt ggtcccaaga caaaagattg
     7321 tgtctccgtc aaacatggcg tgcgacgggc ggatggcgcg ggcaaatccg tcatgggcca
     7381 tctgggcaat tttttgcgcc tgcgctttgg tcaggacggc gttggttgcg accacgccga
     7441 tggttgtgtt ttgtcccgcg gaagccacag gtagcggagg cagcttcacc gctcgctttc
     7501 caagagctcc gaattccgat cccattccct gtctgatttc ccagaagcgc cccgtcgcgg
     7561 gatcgatgac ggagccgaga gagttcacgg caacaatcgc cgcgacgatg attcccgagg
     7621 gcagggcttc gctggccgtt cccaggccgc ctttgatccc tccggccatg gcgcccgttc
     7681 ccgcgccgac ggatccgagg ggaaagccgg cgctgcccgc ggcgtcgcag gctgccgccc
     7741 cccattttgg accgatcggc ggaacgaact gctttccccg gcccagatca aaaagagcgg
     7801 ccgccggcac aatgggcgcc acatggccgt cctgcagcgg aaatccccag cctttctttt
     7861 cgagccggcg caccacgccg tcggccgttg ccagaccata gacggatccg ccggtcaaca
     7921 ccaccgcctg aactttttcc accaagttca tgggctggag caggtccgtt tcgcgggttc
     7981 ccggtgcgga gccgcgaaca tccacgccgg ctgtcgctcc ttccgggctg aggatcgcgg
     8041 tgacgccgct tgcgtatttc cggctggtga aatgaccgac caggattccc tcaacatcga
     8101 tcaaagaatt gtgtcttccc gttttcggca tctgaagttc tccttgattg cttttttcga
     8161 aatatcccgg cccgtcagca tcctgtcgcg atccgctgct gaaagattga acggtcattg
     8221 caaaaccaat ttgcatttta tgtagcacat gtcatttgag agttcagctt ttttttcgtt
     8281 ttcgattcca gtggccacga aggataaact cgtgtatgat gccgcgcatg aaaagaggaa
     8341 gcggcatact gctggcgatt tcttcgctgc ccggacggtt cggcatcgga acgctcgggg
     8401 agggcgcgcg aaaatttgtc gattggcttg ggcaaagcgg gcaggcctac tggcagattc
     8461 tgcctatttc gccgacgggt ttcggcgact ctccgtatca ggcgttcagc gcctttgcgg
     8521 gcaatccgta ttttatcgat ttggaggatc tgcatcggga aggactgctg ccccgcgggg
     8581 cattggacct ttgtgaaagt ttttttgcga gtgatccgga ttccgtggat tattacgcgc
     8641 aatacctggg caggcctcaa gttctttctc ttgccttccg tcatggcgcc gggcggctgg
     8701 ctgccgggct ggaagctttt cgaaagcgcc accaaagctg gctcggcgat tacgcgctgt
     8761 atatggcgct gaaaggcgaa tacgaaatga agcctttcga tcaatggccg gaagcgctgg
     8821 ccctgcgata ccccgccgcc ttgaaggaag ccgcagcccg cctcaaggag caggtcgact
     8881 atcatatctt tgttcaatac cttttctttc gccagtggga cgcgctgcgg aaatatgccg
     8941 gcggcagggg gatccgaatc atcggcgata tgccgatcta catggcgccg gacagcgcgg
     9001 acgcctgggt cgaacacgac attctggatc gcgagaggcg cgtcgcgggc tgcccgccgg
     9061 actacttttc agcaacggga cagctctggg gaaatccgct ttacgactgg gagaaattga
     9121 aggaaacgga atatgcgtgg tggatgcgaa ggatcgccca tcaactgtct ctgtatgatg
     9181 atctgcgcat cgatcatttc cgcggctttg aagcctactg ggcgattccc gccggcgcga
     9241 agacagccga atcggggcaa tggcttcccg gtccgggcat cgactttttc cgccgcctga
     9301 aaaaagcgat cggcgatccg cccctgattg ccgaagacct ggggtatctg acgccggagg
     9361 ttttcgagct gcttcgcgag accggctttc ccggtttgaa agtgcttcat ttcgccttta
     9421 cgccgggcgg ggaaagcatt tacttgccgc accggtatga gaaaaactgc gtggtttata
     9481 cgggcacgca tgacaacgac acaaccgtcg gatggtatcg cggtttagcc aaagatgaac
     9541 tagcttttct ggatgcctat ctggacggcg ttgacgaaga gcgcattcac tggcagttga
     9601 ttcgactggc catgagctcc gtggccgatg tgtgcatttt cccgatgcag gatgtgctgg
     9661 ggcttccgtc ctcggcgcgc atgaaccggc cgcagacggc gcagggaaac tggcggtggc
     9721 gtctgacggc ggacaagctg gatggcgagc tttcgcggaa actggccggt ctgaccgaat
     9781 tattcggccg tcagcggtga agtcatgatc aaaaacgaca ttccgcagga cgagatctac
     9841 ctcttcaata tcggcgaggc gcagcaggcc tacgccactt tcggctgcca ccgcgtggac
     9901 gaatccgatc tgcaccgatt tctcgtctgg gcgcccaacg cgcgggaggt cggcgttgtc
     9961 ggggacttca acggctggga cgcttcccga aaccggatgg agcgccttga ttcgggtgtt
    10021 tttgcggcat ttgtgcccgg ccttcgagac ggggactgct acaagtatgc aatccaaggc
    10081 cacgacggaa aaacggtgct caaggcggat cccttcgcgt ttcatgccga ggttcgtccg
    10141 gcgaccgcct caaaggtctg gagcctgggc ggatacgact ggcgcgatgc ggaatatctg
    10201 aagcggcgcc acggacagaa ccttctgaac cggccggtcg ccatttacga aatgcacatc
    10261 gattcgtggc ggaaacgaga gggatatgcg ttcgcgaact ttcgtgagat tgctgacgag
    10321 ctttccgatt atctggtcga gatgggcttc acgcatgtgg agattatgcc cgttctcgaa
    10381 catccgctgg acgattcctg ggggtatcag gtaaccggtt tttacgcggt aaccagccgc
    10441 tacgggacgc ctcaggattt catgtatttt gtcgatgtca tgcacgccaa aggcatcggc
    10501 gtcatcatgg actgggtgcc ggctcatttt ccccgggacg aacacggcct ggcgcgcttt
    10561 gacggcacct gtctctacga gcatcaaaac ccgatgcagt ccgatcaccc gcagtggggc
    10621 acgctgattt tcaactatgc ccgtcccgag gtggcaagct ttctggtttc cagcgcgatg
    10681 tttttctttg acgtgtgcca catcgacggg attcgcgtgg atgcggtcac atcgatgctc
    10741 tacctggact acgcccgcaa tccgggcgaa tttgtgccca acgaagacgg gggcaacatc
    10801 gaccgctccg cggtggcttt tctgcaaaag ctcaattccg tgattctgac gcgctacccc
    10861 ggcgtcatga ccgttgcgga agaatcgacg gcttacccga tgatcaccaa accgccctac
    10921 gacggggggc tgggtttttt gttcaagtgg aatatgggct tcatgcacga cacgctgagc
    10981 tatatgtcga tggatccctt cttccgccag catcatcaca gcaaactgac gttttccatg
    11041 cactacgcgt ttacggagaa cttcattctg ccgtattccc acgacgaggt ggtccacggc
    11101 aaaaaatccc tgctcaacaa aatggtcggc gcgtacgacg aaaaatttgc gtcgctgcgc
    11161 ctgctgctgg gcttcatgta cgcgcacccc gggaagaagc tgctcttcat gggcggggag
    11221 ttcgggcaat ttatcgagtg ggactaccga aaggaactcg actggtttct gctggggtat
    11281 gaacggcacg gccagatgcg ccggtacgtg caggcgctga acgagtttta tacccgcacc
    11341 ccggcgctgt atgcaatcga cgacgggtgg gaagggtttg cctggctcaa cgtgaacaac
    11401 gcaaagcaga gcgccgtgag ttttctgagg cggggcgcgc gccccgaaga ggtctgcgtg
    11461 tgcgccttca actttacgcc ggtgccggtg gagcggtttg tgatcgggct gccgtccggc
    11521 ggtgcgctgc gggacgtctt cagcagcgac gatgcgagat tcggaggaag cgggcgcggc
    11581 gcggcgcggg tcattcaagc cgtccataaa ggttttgaag gccatccgta ccgcgcggcc
    11641 attgacctgc cgccgctggc ggccgtttat tttgaaatgg attcctatga aaagggtttg
    11701 aaatgaacag cgcattgcag gaaactctgg ctgaaaaaaa aatgaaggct ccgcccccgg
    11761 ttctgatgat cggggcggaa tgcgcgccgt ttgccaaaac gggagggctg gcggatgtga
    11821 tcggaacgct gacgagagag atgaaaaggc tgggattcga tgcccggctg atgctgccgt
    11881 ttcatcgcgt catcaaagac cggtaccggg aacgcgtgcg tcatctggtc agcttcagcg
    11941 tggacatcgg cgggcgatcc cagtatgtgg gaatcgaaca gttcgacggg gaggacattc
    12001 cggtttactt catcgacaac gaacattact tcggtcatgc catttactgc ggcggcaatt
    12061 tcgagggaga gcaatacgcc tacttcacgc gggcggccat cgagtctctt ccaatgattg
    12121 gttttgagcc ccggattctt cacgtgaacg actggcatac ggcgatgatc cccatgctgc
    12181 tgaaaaccca gtatgaccgc ctgccgcagg gacggtgccg cacggttctc tccattcaca
    12241 acctcggcta tcagggacgc ttccatttcg acttcgtttc ccatttgctg ggcatcgaga
    12301 gccggtatta tcatccggac aacatcgaat atcacggcga cgcgaacttt ttaaagggcg
    12361 gcatcgttta cgcggacaaa ctggtcacgg tcagcccgac ttacgcccgg gagatcctga
    12421 cggcgcatta cggcgaaggt ctggagggca ttctggccac acgggcggac gacctgaccg
    12481 gcattctcaa cgggattgat gtcaccgcct tcgatcctgc gtcggatccg tttattccct
    12541 atccgtatga cgcctcgtcg ctttcccaaa agcagaaaaa caagaaggct ctgattgccg
    12601 agcttggctt gaatctttct ccggaagccc ctctcatcgg catggtgacg cggctgacga
    12661 ggcagaaggg gattgacctg gtcctgcgcg tcttcgaaga gattatcgcg gcgggggccg
    12721 gttttgcgct gatcgggacg ggcgatcacg cctatgagca tttcttccgc ggggccgcgc
    12781 accggacggg cggcagggcc gtcggcgtga ccgagttcaa tgatccgctg gcgcgccgca
    12841 tctacgcggg atgcgatttt ttcctgatgc cctctcagtt tgaaccctgc ggcctttcgc
    12901 agatgattgc gctgcgctac gggacgctgc cgattgtccg ggcgacgggc ggcctccggg
    12961 acacggtctt tccctacaac gaatatacaa acgaaggaaa cggcttcgct tttgaaaact
    13021 acaacgcgca cgacatgctt catacgatcc gttacgcact gggcgtctat gaaaacaagg
    13081 agcggctggc ctcgctttgc gtgcgggcga tgagtcagga tctgagtttt caaaaaagcg
    13141 cgcttgccta cgcgaaagta tatgtggatg cggccggaaa atgagacgcg gattttccat
    13201 tgacaggcgc cgggacgtac aataaaacgg tttcccattc cggagggagc ggactctgct
    13261 tccgatccca agagtaaagg cgaacaggtg aaaaatagga ttgagcaaat aaggggaatc
    13321 attgagggaa agctgaaacg gtactacggc cgcgcgctcg agaacgcctc ggacgaggaa
    13381 atgtttcagg tgacggcgat gagcatccgc gacatcatgc tggagcgggg ggtcctggcc
    13441 aacgaaacgg ttgaaaaaaa ggggcacaaa agactctgct acctttcggc ggagtttttg
    13501 atgggaagag ccctggtgaa caatctcatc aatctcggtc tgcttgcgga gtaccgggct
    13561 gtcatggccg acatcggcta cccttttgaa aagctcgaag agcaggaaat ggaagcggcg
    13621 ctgggaaacg gaggcctggg ccggctggcg gcctgttttc tggattctct ttccacgctg
    13681 aatctgccgg tgacgggcta cggcatccgc tatgaatacg gaatgttccg ccagcacatc
    13741 gtggatgggc agcagacgga agttccggac gactggacgg caaagggcga catctgggaa
    13801 gtcgaacgcc gcgaggaaca ggtggaggtt cgctttgacg gcacggtgga ggaggtgtgg
    13861 acggagggcg ggatgacctg cgagcacagg gattaccaga ccgtactcgc ggtgccccac
    13921 gacatgccgg tcatcggctt cgacagccac acacctgcga cgctccggct ctggcgggcg
    13981 gaatgtccgt ccggctttga ccttcactcc ttcaacaagg gtgattttgt ctgcatgctg
    14041 cgtcagaaag agctggccga gtcgatttcc aaggtactgt atcctcagga taatcacatg
    14101 accgggcgca tgctccgcct gcggcagtat tattttctgg cttccgccac gatgcagtgc
    14161 atcgtccgcg
//
LOCUS       DAOSRM010000057        13757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4546_length_13757_cov_112.826303, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000057 DAOSRM010000000
VERSION     DAOSRM010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13757
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4546_length_13757_cov_112.826303"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..164
                     /gene="ffs"
                     /locus_tag="PLA58_06700"
     ncRNA           67..164
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLA58_06700"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            328..2031
                     /gene="dnaX"
                     /locus_tag="PLA58_06705"
     CDS             328..2031
                     /gene="dnaX"
                     /locus_tag="PLA58_06705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOR62521.1"
                     /translation="MEYQVLARKFRPQTFEEVAGQEPVVRTLVNAIAQGRIGHAFLFS
                     GPRGVGKTSVARILAKSLNCEKGPTATPCNKCTNCKEITEGRSMDVREIDGASNRGID
                     EIRELRENVKFAPAASKYKVYIIDEVHMLTREAFNALLKTLEEPPGHVIFIFATTENH
                     KVPATILSRCQCYDFRRISLSQIAANLGRLAAKENIVISATALSWIAEAGDGSMRDAQ
                     SIFDQIISYAGQNISDEEVEDILGLSDRKYLYRLSEAVLARNASDCLSILEEAYLAGV
                     DMKHFYAMLLKHFRNLLLVKIAADGSSSFDIAPEQVEKLKIQTNNTTRETLQRYLEIL
                     LDEADNLRRSQEVRMKIEAVLVRMAYLAPVLPVGEILATLESLEQKIRAGSPAAQGGT
                     DAGPVAAKQPSEYPQTGGAEDDSGSTPQKVAETEARRPAERFGPEDDLKKFVKKENPR
                     LGAKIDAAEVLQLENGCLTLGFPQGYIFLDNLLEKQQKEELERIAGLYYSQKVSLKII
                     TIEAPKANSNGNGRSARPNNLADLKREAVNSPHFQKILAEFDGAELVEIKPITDKNNR
                     G"
     gene            2036..2353
                     /locus_tag="PLA58_06710"
     CDS             2036..2353
                     /locus_tag="PLA58_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOR62522.1"
                     /translation="MHNIGNIMKQAKKMQEKMARLQQELETRTVEAQSGGGMVRVLVN
                     GKFEIVSLTIEKDVVNPEDIDMLQDLIAAAVNEGIRKSQEMAQSEMSKITGGLSIPGL
                     GGL"
     gene            2362..2961
                     /gene="recR"
                     /locus_tag="PLA58_06715"
     CDS             2362..2961
                     /gene="recR"
                     /locus_tag="PLA58_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOR62523.1"
                     /translation="MKSYAQPIKRLIAELAKLPGIGEKTAARLANYILRATPEDVEKL
                     AESLVEVKRKIHLCRICLNPTEEDPCHICGDPSRDSRVICVVEEPDALAAIEESGVFH
                     GTYHVLHGALAPLDGIGPENLRLGELRRRVQEGPIEELILATNPNVHGEATALLIMRM
                     FKDSGMKITRIAMGVPMGGDLKYVDKMTISKSLQFRHGM"
     gene            2987..3853
                     /locus_tag="PLA58_06720"
     CDS             2987..3853
                     /locus_tag="PLA58_06720"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOR62524.1"
                     /translation="MNRINKKSMRRINARKITKAVKTLFIEANTVLGKDVMEALQKAL
                     STEESDTGRQVLQKMIENADIARREKIPICQDTGLAVVFVELGQDVRVTGGPLQEAIE
                     EGVRQAYQDGYLRKSVCDPLTRANTNDNAPSILHMDIVPGNQLRIIAMPKGGGSENMS
                     AARMLTPAAGIEGIKKFVLETVEAAGANPCPPIIVGVGIGGSLEQACILAKKALLRPL
                     DGEHNKDERLARMEDELLKRINALGIGPGGLGGRVTALAVSAEMMPCHIASLPVAVNL
                     QCHVARHKEVII"
     gene            4052..4297
                     /locus_tag="PLA58_06725"
     CDS             4052..4297
                     /locus_tag="PLA58_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62525.1"
                     /translation="MEKTCRKLPAAVVKKYELWKDIIFRHGPEKLREFPGFHDEKLTG
                     KREGQRSSRLSLKYRVIYTVEKEVVTVLVLDITPHEY"
     gene            4407..4685
                     /locus_tag="PLA58_06730"
     CDS             4407..4685
                     /locus_tag="PLA58_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR62526.1"
                     /translation="MKTGKKNEFVPAKPHAQVTTGEVIRMLRELKGWTQSELSERCGI
                     SATNLSLLENDKVEIGKRRVEQLAKAFDVHPAIIMFPEYEGKEIQKAA"
     gene            4708..5268
                     /locus_tag="PLA58_06735"
     CDS             4708..5268
                     /locus_tag="PLA58_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOR62527.1"
                     /translation="MSTTRKIQTPLTEDVIASLHAGDMVLLSGEVYTARDAAHRRLCE
                     ALSQGLPLPIDLATSTIFYAAPSPTPPRRVIGSIGPTTSYRMDAFTPALIEHGLRAMI
                     GKGSRSAQVIESIKKNKAVYFGAIGGIAALTAGRVTKIDLVAYEDLGPEAIRKLSILD
                     LPLVVINDSRGNDLYLSEREKWRTLS"
     gene            5365..5913
                     /locus_tag="PLA58_06740"
     CDS             5365..5913
                     /locus_tag="PLA58_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOR62528.1"
                     /translation="MIEVRWHGRGGQGAVTSVELLAVSAIAAGKYAQGFPSFGPERRG
                     APVAAFTRIDDKKINVRSGIYEPDVVLVLDSSLIGLVPVTDGLKPDGKLIVNTPKTPE
                     EIRRELNFQGTVATVDGTGIARKEMGVPIANTTMIGALLKVIGVMELDAMKDAIDRRF
                     GRIAQKNLNAMKRAYDEIKILN"
     gene            5923..6201
                     /locus_tag="PLA58_06745"
     CDS             5923..6201
                     /locus_tag="PLA58_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR62529.1"
                     /translation="MTLKTWRELPIGCVVSEPGSASEYHTGSWRSQRPIWNDTKCIKC
                     GICYVFCPEGCVQQTEEGFFKANMDYCKGCGICAHECWPRAIKMVEEG"
     gene            6203..7378
                     /locus_tag="PLA58_06750"
     CDS             6203..7378
                     /locus_tag="PLA58_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOR62530.1"
                     /translation="MGKRIGMEVAVAVAEAVGLCNIDMAAVYPITPQSHIAEHLSDLV
                     ADGHIDCEFVTVESEHSAMSAVIGASGAGARSYTATSSQGLMLMHELLPIASAMRLPI
                     VMGIANRAVSGPLNILNDHSDIMPQRDAGWIALFVENAQEAVDLSIAAFKIAEHKDVM
                     LPVSVNIDGFQLTHMVEPFEMPTQEEVNEFLPPFVPHATLHPSKPVTMGAFAMSDYFT
                     EIMKAKDEAVKASKKVIQQVFDEWGEKFGRPYKLVETYKADDADVMMLTMGSMGETAQ
                     MAIDELRAKGVKAGLVKLRLWRPFPFEELKAVIGNAKKLIVTDRACSFGGPGGPVFSE
                     IKSALYAEARRPLIYNYIIGLGGRDVQVKEFVGMFENVLADTANKAADTYEFWGVRE"
     gene            7375..8331
                     /gene="porB"
                     /locus_tag="PLA58_06755"
     CDS             7375..8331
                     /gene="porB"
                     /locus_tag="PLA58_06755"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HOR62531.1"
                     /translation="MMNIVENFDLFAPRLINKQELISSGHRACSGCAEVLAVRLMCKA
                     LGENTVIASATGCMEIISSMFPTTAWQVPWIHVAFENAAAVASGVEAGLKALRKKGKI
                     DDRYVKVVGMAGDGGTMDIGFQALSGAMERGHDMIYVCFDNEAYMNTGIQRSSGTPMG
                     ASTTTAPAGKVSIGQKTWKKNMPEIMVAHGIPYVATACPSYPLDFIRKVEKAKSIQGP
                     SYIHCFSMCPTGWRSPSHTAVSMGRLAVETGVFPLYEVENGHYRLSPDMPKKLKPVKS
                     YFKPQGRFRHLTPDQIEMIQEKVTVEFGKLVEKAKFLKTGNE"
     gene            8368..8853
                     /gene="nuoE"
                     /locus_tag="PLA58_06760"
     CDS             8368..8853
                     /gene="nuoE"
                     /locus_tag="PLA58_06760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOR62532.1"
                     /translation="MTKPISVAAKTRQIVKKHGGGKSGMIAVLQDIQEAFNYLPKEAL
                     KTAAKAMNVPFSRVYEAATFYTAFSLKPRGKHIVKICKGTACHVRGAAILQDRFETTL
                     GIKPGQTTADGKFSLETVNCVGACALGPVVVINADYHGQVTINKVDKIIKKINEEGAQ
                     S"
     gene            8850..10697
                     /locus_tag="PLA58_06765"
     CDS             8850..10697
                     /locus_tag="PLA58_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOR62533.1"
                     /translation="MNENPDTQARQIMDNLTLYYGPKKKFVAICAGTGCRASGSLKVK
                     EALEAELKKQNMGDVDVLATGCLGFCEKGPMMVIHPDKYFYQHVKVEDVPEIISKTVA
                     KGEVISRLLYRHPETQKTLFREEDLPFYKKQHRVVFGQNGMIDPARIEDYFAINGYQA
                     LGKVLSEMTPEAVIGEVKKAGLRGRGGGGFHAAVKWEGVRRAHGDPKYVIANGDEGDP
                     GAYMDRSLMEGNPHSVIEGMIIGAYAVGSNQGYIYVRNEYPLAVTHLTKAIADAREMG
                     LLGKNILGTDFSFDIQITKGAGAFVCGESSALFASVEGRAGEPRAKYVHAVEKGLYDK
                     PTVLNNVETWANVPLIINKGADWYNTIGTEGSKGTKIFSLVGKINNTGLIEVPMGTTL
                     YEIIYDMGGGIPNGKKFKAVQTGGPSGGCIPESLLDIPVDFDKLYEVGSMMGSGGMIV
                     MDETSCMVDVARYFLEFLKEESCGKCVPCREGVRRMSDILEDICAGRGREGDVEILEA
                     MGKAIADGSLCALGGSAPNPVLSTIRYFREEYDAHIRDHRCPAGVCKALISYSIDAEK
                     CTGCGVCVKVCATKAISGEKKKAHVIDKEQCTRCGACIQSCKFDAIHVK"
     gene            10712..11425
                     /locus_tag="PLA58_06770"
     CDS             10712..11425
                     /locus_tag="PLA58_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOR62534.1"
                     /translation="MVNMMIDGKNVSAPKGTMLLESIRGAGIAVPTLCAHEAISRSGA
                     CRLCVVEIKKGNRTRIVTSCLYGVEEGLIVDTKSERVLNVRRLVMELLLSRCPESDVL
                     QKMARDMGVAPQPRYAVDTDKGKCILCRSCVRVCEEVVGVSAIGLFSRGSYKEVGTPY
                     NEKSEVCIGCGACAYVCPTGHIEMISTGDRRKIWGRTFKMQACPKCGKFFAPVDQLKF
                     ISKKTGTSMEELSVCTECR"
     gene            11555..12250
                     /locus_tag="PLA58_06775"
     CDS             11555..12250
                     /locus_tag="PLA58_06775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR62535.1"
                     /translation="MKNSSRWMIRLLTRLRDARLVWDVMGSLYNMRIYPLIDGLYQSI
                     AEELTLQKQAQILEAGAGRGYLSLLLADRNPDARFTGIDYSRMQIRRAERYRREKKIS
                     NCSFRQNNVLNLPFEEAAFDAAVSVGSIKHWSDPHRGLTELLRVIKPGGCLMIAETDR
                     EAPDDELRQFVKRFSIPFVPEGLLFWGLRHVVFGQSLSEDQLADEAGRAGFHSVERRP
                     VNTCPYVMVKAWK"
     assembly_gap    12254..12353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12354..13643)
                     /locus_tag="PLA58_06780"
     CDS             complement(12354..13643)
                     /locus_tag="PLA58_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR62536.1"
                     /translation="MKRTYSKIFLICLSLAFLLAGLAPARTKAADNPWLKQGTDSFEA
                     GQYADAVSACTKAIEQDPNDAGAYKVRGNALAALGRYRQALRDYTKAIELQPEDAQAY
                     EQRGNVQASLGNYEPSKKDLDKAEELKSKMASAHLVKGIEHEKLGEWEAAIAELQKAT
                     ALNPGLALAYYNREIAYRQTGQYENALKDANKAVELAPTFAMAYLNRGVVHGLLGNWR
                     PALDDFNKAIELNPQFEAAYVNRGFAYFNLGDYSGAIREADRAIGLNPKLAIAYYNRG
                     AAYAKLGNAEQALADMKAAAELGHQGAQDFVEKKKIAEAKRQMETAAKQPQKPAGQPD
                     LPVSRDGAASPSLSNAELPIAPILEPSIRTDAPPEVQPRYKNIRGVVLYDGRTVEGQI
                     VSMNTETVNIRTPDGKILSFSFMKEVQNFLKDQGQNP"
BASE COUNT         3257 a         3575 c         4000 g         2825 t
ORIGIN      
        1 ttaaaatttg gttgattcct gtcgatttat ccattaagag tctggccaga agaattgttt
       61 ttacatggcc gggtcccgta caacgaaggc ttgtgaaccc cgtcaggtcc ggaaggaagc
      121 agcggcagca aatcccgtcg tgtgttgcgg caaacccggt caccttttga gagccttgca
      181 caatctgttt tcccgtcatt cccgcgaagg cgggaatccg ggaaagggtt gatcatgctg
      241 gattccggct ttcgccggaa tgactcaggt gttgagtaaa gctctccttt tgtcctgtcc
      301 cggtaaaaac ttcgcaatga ggttcatttg gaataccagg ttttagcccg aaaatttcgc
      361 ccgcagacgt ttgaggaagt cgcgggccag gagccggtgg tccggacgct ggtgaacgcc
      421 atcgcccagg gaaggatcgg ccacgctttt ttattttccg gtccccgcgg cgtcgggaaa
      481 acctccgtgg cgcggattct ggccaagtcg ctcaactgtg aaaaagggcc gacggcaacg
      541 ccctgcaaca agtgtacgaa ctgcaaagaa attaccgagg ggagatccat ggacgtgcgc
      601 gaaatcgacg gcgcgtccaa ccgcgggatc gacgaaatcc gcgaactgcg cgagaatgtc
      661 aaattcgcgc cggccgcatc caaatacaaa gtttacatca tcgacgaagt ccacatgctg
      721 acgcgcgaag ccttcaacgc gcttttgaaa acgctggaag aaccgccggg gcacgtcatc
      781 tttatttttg ccacaacgga aaaccacaag gtgccggcca ccattctgtc gcgctgccag
      841 tgttatgatt tccggcgcat ttcgctttcg cagattgcgg ccaatctggg caggcttgcc
      901 gcaaaggaaa acatcgtcat cagcgccacg gccctttcct ggatcgccga ggcgggagac
      961 ggctccatgc gcgatgccca gagtattttt gaccagatca tctcctatgc cggccagaat
     1021 atttcagacg aggaagtgga ggacatcctg gggctttccg accgcaagta tctgtaccgc
     1081 ctgtccgaag cggttttagc ccgcaacgcc tcggattgtt tgagtatttt ggaagaagcc
     1141 tacctggcgg gcgtcgatat gaagcacttc tatgcgatgc ttttgaagca tttccgcaat
     1201 ttgcttctgg tcaaaattgc cgcggacgga agttcgtctt ttgacattgc gccggaacag
     1261 gtggaaaagc tgaaaattca aacgaataac acgactcgtg aaacgctgca gcgctatctg
     1321 gagattttgc tcgatgaggc cgacaatctg cgccgcagcc aggaagtgcg gatgaaaatc
     1381 gaggccgttc tggttcggat ggcctatctg gcgccggttc tgcccgtggg agaaattctg
     1441 gcgaccctgg agtcgctgga acagaagatc agggcgggtt cgccggctgc gcagggcgga
     1501 acggacgcgg ggccggttgc ggcaaagcag ccgtcggaat acccgcagac gggcggcgcc
     1561 gaggacgatt ccggttcgac cccgcaaaag gttgcggaga cggaggcgcg ccggcctgcc
     1621 gaaagattcg ggccggagga tgatctgaaa aaatttgtca aaaaagagaa tccgcggctg
     1681 ggggcgaaaa ttgatgccgc ggaagtgctg cagctcgaaa acggctgcct gacgcttggt
     1741 ttcccccagg gctatatttt tctcgataat cttttggaaa aacagcagaa agaagaattg
     1801 gaaagaatcg cagggttgta ttattcacaa aaagtttctt tgaagattat caccatcgag
     1861 gcgccgaagg ccaacagcaa cggcaacggg cgaagcgcaa ggccgaacaa ccttgccgac
     1921 ctcaagcgcg aggctgtgaa ttctccgcat tttcaaaaga ttctcgccga atttgacggc
     1981 gcggagctgg tggaaatcaa accgattacg gataaaaaca acagggggta gcaaagtgca
     2041 caatatcggc aacatcatga agcaggccaa gaagatgcag gaaaagatgg cccgtctcca
     2101 gcaggagctt gaaacgagaa ccgtggaagc gcagtcgggc ggcggcatgg tgcgggttct
     2161 ggtcaacggc aaatttgaga ttgtatcgct cacgattgaa aaagacgtgg tcaatccgga
     2221 agacatcgat atgcttcagg atctgattgc cgcggccgtg aatgaaggca tccgcaagtc
     2281 acaggaaatg gcccagtcgg agatgtccaa gataacgggc ggcctgtcca ttccaggatt
     2341 gggcggtctg taaatcatcc tatgaaatct tacgcgcaac ccattaaacg cctgattgcg
     2401 gagctggcca agcttccggg catcggagag aagacggcgg cccggctggc gaattatatt
     2461 ttgcgggcca cccccgaaga cgtggaaaag ctggcggaaa gcttagtcga ggtcaaacgc
     2521 aaaatccatc tttgccggat atgcctcaat cccacagagg aagacccgtg tcatatctgt
     2581 ggagatcctt cccgggacag ccgggtgatc tgcgtggtgg aagaaccgga tgcgctggcc
     2641 gccatcgagg aaagcggcgt tttccacgga acgtaccatg ttctgcacgg cgcgctggcg
     2701 ccgctcgacg gcatcgggcc ggaaaatctg cgtctcggcg aactgcggag aagagttcag
     2761 gaagggccga tcgaagagct gattctggcc accaatccga atgttcacgg cgaggccacg
     2821 gcgcttctga tcatgcggat gttcaaagac agcggcatga aaatcacccg catcgccatg
     2881 ggcgtgccga tgggcgggga tttgaaatac gtcgataaga tgacgatatc gaagtcgctg
     2941 cagtttcgtc acggcatgta gcatgacgcc tgaaaacagt catattatga ataggatcaa
     3001 caaaaaaagc atgcggcgga tcaacgcaag gaaaatcacc aaagccgtga agacgttgtt
     3061 cattgaagcc aacaccgttt tgggaaaaga cgtgatggaa gcgctgcaaa aggcgctctc
     3121 cacggaagag tcggacaccg gcaggcaggt gttgcagaaa atgatcgaga acgcggatat
     3181 cgcccggcgt gagaaaatcc ccatctgcca ggatacggga ctcgcggttg tttttgtgga
     3241 gctggggcag gatgtgcgcg taaccggcgg accgttgcaa gaggcgattg aagaaggcgt
     3301 gcggcaggca tatcaggacg gctatttgcg caaatccgtc tgcgacccgc tcacgagagc
     3361 caacaccaac gacaacgcac cgtctatcct tcatatggat atcgtgcccg gcaatcaatt
     3421 gcgaatcatt gccatgccca aaggcggcgg cagtgaaaat atgagcgcgg cccggatgct
     3481 gacgcccgcc gcgggaatcg aaggcatcaa gaaatttgtt ctggaaacgg tggaggctgc
     3541 cggcgccaac ccctgcccgc cgatcattgt cggcgtcggg atcggcggat cgctggagca
     3601 ggcctgcatt ctggcgaaaa aagcgctctt aaggccgttg gatggcgaac ataacaagga
     3661 tgagcgtctg gcgcggatgg aagacgaact tctgaagcgg atcaacgccc tgggaatcgg
     3721 tcccgggggg ctgggtggca gggtgacggc cctggccgtt tccgccgaga tgatgccctg
     3781 ccatatcgct tctcttcccg tggccgtcaa tctccagtgt catgtcgccc gccacaaaga
     3841 agtaatcatt taagcaggaa cagaggtgga tgatgtggat attgcaggga ctgctatccg
     3901 agcagtccga agcgcggctc tgtaatgtcc ttgcgagttc ataagctgag aaacggccat
     3961 attttcaaat gctgtttgaa cctgatcttg acttattata attgataggt tattaaaact
     4021 gataatgtgg catatcaaag agcatcgcga catggaaaaa acatgccgaa agctgcccgc
     4081 cgcggttgtt aagaaatacg aactgtggaa ggatattata tttcgccatg ggccggaaaa
     4141 attgagggag tttccggggt ttcatgacga aaaactgacg gggaaacgcg aggggcagcg
     4201 ttcatcgcgt ttgagtttga agtacagggt aatttatacg gtggagaagg aagttgtgac
     4261 ggttcttgtg ttggatatta cgcctcacga atattgaaaa tgagggataa cagcacccga
     4321 tacattaaac tggattgccc aatggagttg ggcaataaca aagaatgatt catgaattta
     4381 tctcggtgcc agagttggga ggctttatga aaacgggtaa aaaaaacgaa tttgttccgg
     4441 caaaaccgca cgcacaggtt acaaccggcg aagtcattcg tatgcttcgt gaattaaaag
     4501 gctggaccca gtcggaactt tctgaacgtt gcggaatcag tgcgacaaat ctaagtcttt
     4561 tagaaaatga taaggttgaa ataggaaaga gacgcgtaga acagttggca aaagccttcg
     4621 acgttcaccc ggcgattatc atgtttccgg aatacgaagg caaagaaatt caaaaggccg
     4681 cgtagcgaat tcagtgaacc tgaaaatatg agtacaacac ggaaaattca aacccctctg
     4741 acggaagacg tcattgcctc gctgcacgcg ggggatatgg ttttgttgag cggagaggtt
     4801 tatacggccc gcgacgcggc gcaccgtcgt ttatgcgagg cgctttctca gggccttccc
     4861 ctgccgatcg atcttgcgac atcaacgata ttctacgccg ccccatcgcc cacgcctccc
     4921 cgaagggtga tcggttccat cggcccgaca accagttacc ggatggatgc ctttacgccg
     4981 gcactcattg agcatggctt gagagccatg atcggcaagg gcagccgctc cgcgcaagtc
     5041 atcgaatcga tcaaaaaaaa taaggcggtt tatttcggcg cgatcggcgg cattgcggcc
     5101 ctgaccgcgg ggcgcgtcac aaaaatagac ctggtcgctt acgaagacct ggggccggaa
     5161 gcgattcgaa aactttcgat tcttgacctg cctctggtgg tgatcaacga ttcccggggc
     5221 aatgatttgt acctgtccga acgggaaaag tggcgtacgc tttcgtaatc catacgcttt
     5281 tgcagagtcg agaaaagata ttgactcaaa aacccttcta tattaaggga aacggtcatt
     5341 ttatatttaa ggagagtggg atcgatgatt gaagtcagat ggcatggccg gggcggtcag
     5401 ggagcggtga cctctgtgga attgttggcg gtatcggcaa tcgccgccgg aaaatatgcg
     5461 cagggctttc ccagtttcgg cccggaaaga agaggggcgc ccgtcgccgc gttcacacgc
     5521 attgacgaca agaaaattaa cgtgcggtcg ggcatttatg agcccgatgt cgtgctggtg
     5581 ctcgattcca gtctgatcgg tctggtgccc gtcacggatg gattgaagcc ggacggaaaa
     5641 ttgatcgtaa acactccgaa aacgcccgaa gagattcgca gggaattgaa cttccagggc
     5701 acggttgcca cggtggacgg caccgggatc gcccgcaagg aaatgggtgt gccgattgcc
     5761 aacaccacca tgatcggcgc gctgttgaaa gtgatcggcg tcatggaact cgacgcgatg
     5821 aaagacgcca ttgaccgccg gttcggcagg atcgcccaga agaatctcaa cgcgatgaag
     5881 cgggcctacg acgaaatcaa aattctgaat taagaggtac gtatgacatt aaagacatgg
     5941 cgggaacttc ccatcggctg tgtggtgtcc gaaccgggca gcgcaagcga ataccatacg
     6001 ggaagctggc ggtcgcagcg gccgatctgg aacgacacaa aatgcatcaa gtgcggcatc
     6061 tgctacgtgt tttgtccgga aggctgcgtt cagcagacgg aagaaggttt tttcaaggcg
     6121 aacatggatt attgcaaggg ctgcggcatc tgcgcgcacg aatgctggcc ccgcgcgatc
     6181 aagatggtcg aggagggata acatgggcaa acgcattgga atggaagtgg cagttgccgt
     6241 tgcggaagcc gtggggctgt gcaacatcga catggcggcc gtttatccca ttacccccca
     6301 gtcgcacatt gcggaacatc tttcggatct cgttgccgac ggccatattg actgcgaatt
     6361 cgtgacggtg gaatccgaac attcggccat gagcgcggtg atcggcgcgt ccggcgccgg
     6421 cgcccgttcc tatacggcga cgagttccca ggggttgatg ctgatgcacg aactgctccc
     6481 cattgcttcg gcgatgcggc tgccgattgt gatgggcatt gccaaccgcg cggtctccgg
     6541 tccgctcaat attttgaacg atcattccga catcatgccg cagcgcgacg ccggctggat
     6601 tgcccttttt gtggaaaacg cccaggaagc ggtggacctg tccatcgcgg cgttcaaaat
     6661 cgccgaacac aaggacgtca tgctgccggt ttccgtcaat atcgacgggt tccagctcac
     6721 ccacatggtc gagcctttcg agatgcccac gcaggaagaa gtgaatgagt ttctgccgcc
     6781 gtttgttccg catgcgacgc tgcatccgtc caaaccggtg acgatgggcg cttttgccat
     6841 gtcggactat ttcacggaaa tcatgaaggc caaggacgaa gccgtcaaag cctccaaaaa
     6901 agtgattcag caggtctttg atgaatgggg cgaaaagttc ggccgcccgt ataaactggt
     6961 ggaaacctac aaggccgacg acgcggacgt gatgatgctg acgatgggct ccatgggaga
     7021 aaccgcgcag atggcgattg acgaactgcg cgccaaaggc gtcaaggccg gtctggtcaa
     7081 gcttcggctc tggcggccct tcccgtttga agagctcaaa gccgtcattg gaaacgcaaa
     7141 gaaactgatc gtcaccgacc gcgcctgctc cttcggcggc cccggcggtc ccgtcttttc
     7201 ggaaatcaag tcggccctgt atgccgaagc cagacgtccg ctgatctaca actacatcat
     7261 cggcctgggc ggacgcgacg tgcaggtgaa ggaatttgtc ggcatgttcg aaaacgttct
     7321 cgcggacacc gccaacaagg cggcggacac ctatgaattt tggggggtga gagaatgatg
     7381 aatatcgtag aaaattttga tttatttgcc ccgcgtctga tcaataaaca ggaattgata
     7441 tcgtcgggcc accgcgcctg ttccggctgc gctgaagtgc tggccgtgcg gctgatgtgc
     7501 aaagcccttg gagaaaacac cgtgatcgcg tccgcaaccg gctgcatgga aatcatctcg
     7561 agtatgtttc cgaccacggc gtggcaggtg ccctggattc acgtggcttt tgaaaacgcc
     7621 gcggccgtgg catccggcgt cgaagcgggt ctgaaggccc tgcgcaaaaa agggaagatt
     7681 gatgatcgtt atgtgaaagt ggtcggcatg gccggcgacg gcggcacgat ggacatcggc
     7741 ttccaggccc tttccggcgc gatggaacgc ggacacgaca tgatctacgt ctgcttcgac
     7801 aacgaggcgt acatgaacac gggcattcag cgctccagcg gcacgccgat gggcgcctcc
     7861 accaccaccg ctccggccgg gaaagtaagc atcggccaga aaacctggaa aaagaacatg
     7921 ccggaaatta tggttgcgca cggcatcccg tatgtcgcca ccgcctgccc cagttatccg
     7981 ctggatttta tccggaaggt ggaaaaggcc aaaagcattc aaggcccttc ctatattcac
     8041 tgcttctcca tgtgcccgac cggttggcgc agtccttccc atacggccgt atccatgggg
     8101 cgtctggccg tggaaacggg tgtcttcccc ctgtacgaag tcgagaacgg ccattaccgg
     8161 ctgtctccgg acatgcccaa aaaactcaaa ccggtaaaga gttacttcaa gcctcaggga
     8221 cgtttccgcc acctgacacc ggatcagatc gaaatgattc aggagaaagt aacggttgag
     8281 tttggaaagc tcgtggaaaa agcaaaattt ctcaaaaccg gcaatgaata aaagagaaat
     8341 tgaaaagaat accgaggtga aagaagaatg acgaaaccga taagtgttgc agcaaaaacc
     8401 aggcagattg tcaaaaaaca cggaggcggc aaaagcggaa tgatcgccgt gttgcaggat
     8461 attcaggaag ccttcaatta ccttccgaag gaagcgttga agacggcggc caaagcgatg
     8521 aacgtgcctt tcagccgggt ttatgaagcg gcgacgtttt acaccgcgtt cagtctgaaa
     8581 ccgcgcggga agcatatcgt caaaatctgc aagggaaccg cctgccacgt ccgcggcgca
     8641 gccatccttc aggaccggtt cgaaactacg ctgggcatca agccggggca aacgacggcg
     8701 gacggaaaat tttcattgga aaccgtaaac tgtgtcggcg cctgcgcgct gggaccggtg
     8761 gtggtcatca atgccgatta tcacgggcag gtcaccatca acaaagtgga taaaatcatc
     8821 aaaaagatca atgaagaggg ggcgcagtca tgaacgaaaa tccggatact caagcccgcc
     8881 agatcatgga taatttgacc ctgtattacg gtccgaagaa aaagtttgtt gcgatctgcg
     8941 ccggcaccgg ctgtcgcgca tccggcagcc tcaaggtgaa agaagcgctg gaagccgaat
     9001 tgaaaaagca aaatatgggt gacgtggacg tgctggccac cggatgcctg ggattctgcg
     9061 aaaaaggccc catgatggtc atccatcccg acaaatattt ttatcagcat gtgaaagtcg
     9121 aagacgttcc ggaaatcatc tccaaaaccg ttgccaaagg cgaagtgatc agccgcctgc
     9181 tgtaccgcca tccggaaacc caaaaaacac ttttccgcga ggaagacctg cccttttaca
     9241 aaaagcagca ccgggtggtt ttcggccaga acggcatgat cgatccggcg cgcattgaag
     9301 attattttgc catcaacggc tatcaggcgc tgggtaaagt gctttccgaa atgacgcccg
     9361 aagccgttat cggcgaagtg aaaaaagccg gcctccgggg ccgcggcggc ggaggatttc
     9421 acgcggccgt caagtgggaa ggcgtgcgca gggcgcatgg cgatcccaag tatgtcatcg
     9481 ccaacggcga tgaaggcgat cccggcgcct acatggaccg cagcctcatg gaaggcaatc
     9541 cccacagtgt gatcgaaggc atgatcatcg gcgcttacgc ggtcggctcg aaccagggct
     9601 acatctacgt ccggaacgaa tatccgctcg ccgtaacgca tctgaccaag gcgattgcgg
     9661 acgcaaggga aatgggcctt ttgggaaaaa atattctggg cacggatttc tcctttgata
     9721 ttcaaatcac caagggagcg ggtgcgttcg tctgcggcga atcctccgcc ctgtttgcct
     9781 ccgttgaagg ccgcgccggc gagccgcgcg ccaagtatgt ccacgcggtg gaaaagggcc
     9841 tgtacgacaa gcccaccgtt ctcaacaacg tggaaacctg ggccaacgtt ccgctgatca
     9901 tcaacaaagg ggcggattgg tacaacacca ttggaaccga aggctcgaag ggaacgaaaa
     9961 tcttctcgct ggtcggcaag atcaacaata cggggctgat cgaagttccg atgggcacga
    10021 cgctctatga aatcatctac gacatgggcg gcggcattcc caacggcaaa aagttcaaag
    10081 ccgtccagac cggcggcccc tccggcggct gcattccgga gagcctgctg gatatccccg
    10141 tggatttcga caagctctac gaagtcgggt cgatgatggg ctccggcggt atgatcgtca
    10201 tggacgaaac atcctgtatg gtggacgtag cccggtactt cctggaattc ctcaaagaag
    10261 aatcctgcgg caagtgtgtg ccctgccgcg aaggcgttcg ccggatgagc gacattctgg
    10321 aagacatctg cgcgggaaga ggcagggaag gagacgtgga gatcctggag gccatgggaa
    10381 aggcgattgc cgacggctcg ctgtgcgctt taggcggttc ggcccccaac ccggttttga
    10441 gcacgatccg gtatttccgg gaagaatatg acgcgcacat ccgggatcac cgctgtccgg
    10501 ccggtgtctg caaggcgctc atcagctaca gcatcgacgc ggaaaaatgc acaggctgcg
    10561 gcgtctgcgt caaagtctgc gccacgaagg cgatttccgg cgaaaagaag aaagcgcatg
    10621 tcattgacaa agaacaatgc acccgttgcg gcgcctgtat ccagagctgc aaatttgatg
    10681 caattcatgt gaagtaaagg tggagtaagc catggtaaat atgatgatag acggaaaaaa
    10741 cgtatcggcg cccaaaggca cgatgcttct ggaatccatt cgcggcgcgg gcattgccgt
    10801 tcccaccctc tgcgcgcatg aagcgatctc ccggtccgga gcgtgccgcc tttgcgtggt
    10861 ggaaatcaag aagggaaacc ggacccggat cgtcacttcc tgcctctacg gcgtggaaga
    10921 aggtctgatc gtggacacca aaagcgagcg ggtcctcaac gtccgccggc tggtcatgga
    10981 attgcttctg tcgcgctgtc cggaatccga cgttctgcaa aagatggccc gggacatggg
    11041 cgtggcgccg cagccccggt acgccgtcga tacggacaag ggcaagtgca ttttatgccg
    11101 ctcctgcgtg cgcgtctgcg aagaagtggt cggcgtaagc gccatcggct tattctcccg
    11161 ggggtcttac aaagaggtcg gcacaccgta caacgaaaaa tcggaagtct gcatcggctg
    11221 cggcgcgtgt gcctatgtct gccccaccgg gcatatcgaa atgatctcga ccggcgaccg
    11281 ccgcaagata tggggacgca cctttaaaat gcaggcctgc cccaagtgcg gcaaattctt
    11341 cgctcccgtg gaccagttga agttcatcag caagaaaacc ggcacgtcca tggaagagct
    11401 gtccgtctgt acggaatgcc gttgaaccgt tcagcattcc ccctgaaaaa tgagcccggt
    11461 cctgaaattt tcagaccggg cttttttaag gaaaacctgc tttgatgaaa gcatccccca
    11521 acagtttcgg cattcaccgg gaggaaaggg acccatgaag aattcgagcc gctggatgat
    11581 ccggcttttg acccggctgc gtgacgctcg cctcgtctgg gatgtcatgg gatcccttta
    11641 caacatgagg atataccctc tgattgacgg actctaccaa tccatcgcgg aagagctgac
    11701 cctgcaaaag caagcgcaga tcctcgaggc gggcgcgggc cgggggtacc tgtcgcttct
    11761 gctcgctgat cgcaatccgg acgcccggtt tacggggatc gattactccc ggatgcagat
    11821 tcgcagggcc gagcgctacc gccgggagaa aaaaatctcc aactgttctt tccggcagaa
    11881 caatgttttg aatctgccgt ttgaggaagc ggcctttgac gcggcggtca gcgtgggatc
    11941 gatcaagcac tggtccgatc cccaccgggg actgaccgag ctgctgcggg tgatcaaacc
    12001 gggcggctgc ctgatgatcg cggaaacgga ccgggaagcg ccggacgacg aactccggca
    12061 attcgtcaaa cgcttttcca ttccttttgt gccggagggt cttcttttct ggggactgcg
    12121 ccacgtcgtc ttcggacaga gcctgtcgga agatcaactg gcggacgagg cgggccgggc
    12181 cggatttcat tcagtcgaac gcaggcccgt gaacacctgt ccctatgtca tggtaaaagc
    12241 ttggaaatga aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctaggga
    12361 ttttgtccct gatccttcag gaaattctgc acttctttca taaaggaaaa ggacagaatt
    12421 ttcccgtccg gggtgcggat attcacggtt tcggtgttca tgctgacaat ttgcccttcg
    12481 acagttctgc cgtcataaag aacgacgccc cggatgtttt tatagcgcgg ctgaacttcg
    12541 gggggcgcat ccgtcctgat tgacggttca agaatcggcg caatggggag ttccgcattt
    12601 gacagggacg gcgaagccgc cccgtcccga ctcacaggaa ggtccggctg cccggcgggc
    12661 ttttgcggct gctttgctgc ggtctccatt tgtctttttg cttcggcgat cttctttttc
    12721 tccacaaaat cctgtgcgcc ttgatgcccc agctccgcag ccgccttcat gtccgccagg
    12781 gcctgctctg cattgcccag tttggcataa gcggcgcccc ggttgtaata ggcgatggcc
    12841 agtttcgggt tgagcccgat cgcccgatcc gcttcccgga ttgcccccga ataatcgccg
    12901 aggttgaaat aggcgaatcc ccggttgaca taggccgctt caaactgcgg gttgagttcg
    12961 atggccttgt tgaagtcatc cagcgccgga cgccagttgc ccaggagccc gtgaacgacg
    13021 ccccggttca ggtaagccat ggcaaacgtt ggcgccagtt caacggcttt gttcgcgtct
    13081 ttcagcgcgt tttcgtattg ccccgtctgt cggtaggcga tttcgcggtt gtagtaggca
    13141 agagccagcc ccggattgag agcggtcgct ttttgcaatt cggcgatggc cgcttcccac
    13201 tcgccgagtt tttcgtgctc gattcctttg acaagatggg cggaggccat tttcgacttc
    13261 agctcttcag ccttgtccaa atcttttttc gacggttcgt agtttccaag actggcctga
    13321 acatttcccc gctgctcgta ggcctgtgcg tcctccggtt ggagttcgat ggcttttgtg
    13381 taatcccgga gcgcctgacg gtagcggccg agcgccgcca gggcgtttcc ccggacttta
    13441 taggccccgg cgtcgtttgg atcttgttcg atggcttttg tgcaggcgct gaccgcgtcg
    13501 gcatactgcc ccgcctcaaa ggaatcggtt ccctgtttga gccagggatt gtccgcagct
    13561 ttcgtccggg ccggcgcgag accggcgagc agaaaggcca gcgaaagaca gatgaggaaa
    13621 atttttgaat atgtgcgttt cacgggcttg cttccttcct caaaaatgca cgcggtcatc
    13681 cgggctccgt ctcaaaatag gcggcgccca atttttgttg cagtgtagca taagtggacg
    13741 gattttgaaa atttttc
//
LOCUS       DAOSRM010000058        13747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4552_length_13747_cov_110.839760, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000058 DAOSRM010000000
VERSION     DAOSRM010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4552_length_13747_cov_110.839760"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..2385
                     /gene="fdhF"
                     /locus_tag="PLA58_06785"
     CDS             106..2385
                     /gene="fdhF"
                     /locus_tag="PLA58_06785"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:754..756,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOR62537.1"
                     /translation="MITRDLNKCILCGRCIRACNEIQVNEVLDFANRGSYAKVGPAFD
                     ADYIDSSCVFCGECAQVCPTGAITFKQAKFAGRPWELSKTRTTCAYCGVGCQIDLYTK
                     DNKIVKVMGNRQYGPPNEGSLCVKGRFGMDFISHPDRISKPLIRHQKGEAFKEAAWDE
                     AYAFIAQRLAAVKKEYGPDSIAGLSSARCTNEENYVFQKFLRGGIGTNNIDHCARLUH
                     SVTVAGLAATFGSGAMTNSIDELESTDLILATGTNTTENHPVIGAKVKRAVRRHGTKL
                     IVIDPREIDLVKYADIWLRQKPGTDVAVFNGLMNVIIAEGLYDKEYVAGRTEGFEALK
                     AVVAKYTPESVEKISGVPAEDLKKAARMYAQAGRASIIYAMGITQHITGTDNVKSTAN
                     LSMLCGNVGIEGGGVNPLRGQNNVQGACDMGALPNVYPAYQPVAGEDVRKKFEAAWKA
                     ALSPKPGLTLMEIMAAAGSGKIKALYVMGENPLLSDPDLQHVAKELQKLDLMIVQDIF
                     LTETAQMADVVLPACAFAEKEGTFTNTERRVQRVRKALEPPGEARADWEIICGISSRM
                     GYPMTYASAQEIFEEIRTVTPSYAGITYDRLEKEGLQWPCPTVSHSGTKFLHKDKFSR
                     GLGLFTALEYIPPNELPDQEYPFLLSTGRVLYHYHTGTMTTRARGPVERCPESLVEIH
                     PEDAGKLGIADGQKVRVTSRRGTVEVKARITEKSAPGSIFMNFHFTEAPVNFLTNPAL
                     DPTGKIPEYKVCAVKLEAA"
     gene            2645..5347
                     /gene="fdhF"
                     /locus_tag="PLA58_06790"
     CDS             2645..5347
                     /gene="fdhF"
                     /locus_tag="PLA58_06790"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3707..3709,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOR62538.1"
                     /translation="MSDTFILNGKETPYTPGQMILEAAKVGGIEIPTLCYLKGSTPTG
                     ACRICLVEVKGARSLMAACSTPVTPGMEVFTETALVHQARKLNVELLLSSGTHNCLTC
                     EANGNCRLQDLAYFYKITDLRFEEKAASDIYPKEDRNPLIVRDFSRCILCGRCVQACN
                     EVMVNRAISQGYRGKESKIVTGGDLPYHESECVFCGQCVEVCPVGALTEKKAKGLGRP
                     WEIKKIRTTCPYCGVGCQQWLHVKDGKIVKVTAVEDAQPNMGRLCVKGRFAYDFIYSE
                     ERLKTPLIREANGEFREASWDEALDLVAEKFKEIIKKDGPDAIAGVSCARSINEDSYQ
                     MQKFFRAVIGTNNIDHCARTUHAPTVAGLATSFGSGAMTNSFADYAKAKMIILVGTNI
                     TEAHPVASTFVKDAVLAGAKLIVIDPRRTDMVDFAEMHVPLKVGSDVALINGVMNVLI
                     EENLYDKKYVDSCTEGFEDLKKKVMEYPPERAAEISGIPADTIRELARKMASVKPAML
                     MYTLGITEHICGVNNVMSCANLQMLLGNVGVECGGVNPQRGQNNVQGACDMGALPNVF
                     PGYQQVANADARAKFAKAWNVKSLPDKPGMMMPTMMKNLTTGKVKAFYVFGENLANTE
                     PDIHHVERELQSAEFLVCQDIFPTETTRFAHVILPAAAWSENDGTFASSERRVNRVRA
                     ASVPPGDARPNWWIFKELAKRFGHEWQSSSAQEIWDNEISVLAPSMAGIKYARLENDG
                     LQWPCPTEDHPGTPTLHKGGKFTRGLGMLKAIDYIPPAEVPDQEYPFVLSTGRRLYHY
                     HTRTQTGRSAGINDLLGEETADISTADAEAKGISDGEKISVKSRRGEVVVKARVTKEV
                     PPGLVWMAFHFREACANWLTNPVYDPVTQTAEYKACAVRIDKLR"
     gene            5446..6189
                     /gene="modA"
                     /locus_tag="PLA58_06795"
     CDS             5446..6189
                     /gene="modA"
                     /locus_tag="PLA58_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOR62539.1"
                     /translation="MKKTILVVMMLSILAAPFHVFAQDKLSVAVAANFISAFKEIAAA
                     FEEQTGVKVEATFSSTGNLYSQIKNGAPYDVFLSADEQRPDLLQKEGWTDGTFIYARG
                     QAILWSADPNFCKAPTWQEALKNNRIKKISIANPVTAPYGAAAKAALQKAGLWDALQD
                     KLVNGQDIAQAFQYASTQAVDAGFCALSAAASPQGKNGCFFSIPEAPDIVQAGCILKK
                     SKNQKCAELLMAYLLGADAAKTKKKYGYR"
     gene            6192..6851
                     /gene="modB"
                     /locus_tag="PLA58_06800"
     CDS             6192..6851
                     /gene="modB"
                     /locus_tag="PLA58_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOR62540.1"
                     /translation="MDYLALYVTLKLALVTTLVLMVLAAPVAYLLAYARFPGKSLVEA
                     LIYLPMALPPTVIGFYLIIVMGPRGWAGRVWEAATGGSLLFTFLGITIASVVYSVPFA
                     VQPMKAAFQKIDRRLLENAYILGLSKRAAFLRVIIPNSVAGIAAAAVLVFLHSIGAFG
                     VLLMVGGSIPGETKVASIAIYEAVEMMNYEAAGLIALSFIPISYAFLLLINRLNARAG
                     A"
     gene            6848..7561
                     /locus_tag="PLA58_06805"
     CDS             6848..7561
                     /locus_tag="PLA58_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR62541.1"
                     /translation="MTLSASFRKRLKYFDLEISFACRAGKMLVMIGPSGGGKTTVIRM
                     LAGLTMPDAGRVVFGDEVWFDSARNIHLKPQKRRLGYVFQDYSLFPHLNLYDNAAFAA
                     ESKKEVDELFELFKISHLKKRKPHMVSGGERQRCAICQALARHPRLLLLDEPFSALDV
                     VTRRGLREELKILKGRLFCPVIYVTHDIHEALFLADEILPVVDGKIDRGWMQRTITRE
                     PGEGISARGAREPRLSLVY"
     gene            7620..8213
                     /locus_tag="PLA58_06810"
     CDS             7620..8213
                     /locus_tag="PLA58_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HOR62542.1"
                     /translation="MNICTYSYEEYLHLVKSFHGNLAPGLIIGGFIVDLAMKHLPAGD
                     FFDAVCETPVCLPDAVQILTPCTIGNGWLSVVPFGKFAVTLYEKYSGRGVRVYLDMEK
                     LKAWPEIHDWYLKKKKKSEQNPDVLLAQIKEAGHQLLSIQQVQVEPEKVRRRKMGPVA
                     VCPVCGEAYPTRDGDKCRSCQGETPYKDVVAVDAIKS"
     gene            8223..8990
                     /locus_tag="PLA58_06815"
     CDS             8223..8990
                     /locus_tag="PLA58_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOR62543.1"
                     /translation="MSLQPGTLLNRLYERLEPDAKNRRIAELRIGLSYVGVRLDHGGT
                     GLAAVLPDAASGGCTVLPAAGKLAGSSAADLLAYLPEGKNAVERAVGLAAANALIASS
                     CDASEDREATTYFGLKPGEKVAMVGLFSPLVERIRATGAILTVIEKNPERLDLLPPDQ
                     KQQALAGCDVAIVTATTLLNHTLEETLAALASPRVVAVIGPSTPLLPAVFSGTPVTHL
                     GGAVVADSTRVLQIISEGGGTPALRPSLRFVNLTIGP"
     gene            complement(9012..10346)
                     /locus_tag="PLA58_06820"
     CDS             complement(9012..10346)
                     /locus_tag="PLA58_06820"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR62544.1"
                     /translation="MTEIKRASRKELFGWAMFDFANSSYTTVIITVVFCIIFPRLIVG
                     DGPEFRLGNLLWSASISISYLIVLLTAPLLGAIMDYTGSKKKFLLGSCWLCALATASL
                     YFVGPGDILIGMLLIIVSNIGFSYSEAFISSFLPGLGPSRDLGKISGYAWGLGYFGGL
                     ASTAIVIFGLGAGVYTLENFSNLRLVGPATGLFFLIAAIPTFLWVREQTSPRALPGGE
                     TYLKIGLKRLKRTLRDIRDYKDLIVLLSSFFFAYAGLSIVISFAFIYGDQVIRWSGKT
                     QILMFVITQFTAAGGALLFGILQDTWGAKRTFILTLIIWVAAVSLIYGVAPVTAWVSS
                     LLGTPLREEHIFLIIGSIAGLGLGSTQSACRAMVGLFSPDTKAGEFFGLWSLTSRLSA
                     IAGLMGLGLLQTLFGLHQAILVCSAFFLIAVVIVLFVNEERGKTVAREHAGE"
     gene            complement(10378..11208)
                     /locus_tag="PLA58_06825"
     CDS             complement(10378..11208)
                     /locus_tag="PLA58_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOR62545.1"
                     /translation="MALVRERDGKRLHVKSRLMGESLVGRTFMDGLAVAPQKRLFPDV
                     NVVKIGGQSICDRGAQALPAIVKEIAACSRKHKILLTTGGGTRSRHIYAIGLELGMPT
                     GVIAKFGSSISEQNALLVAMLLTPWGGIKIGHDDVTKLYTYFSQGCIPVMHGMPPYDY
                     FALPAPKRRIPIHRTDVGTLIAADLIGARRCIFVKDERGLYTDDPKKNSRAAFIPEIS
                     VKELLARDLDDLIIERPCLEILQNSEVIRTIQIINGLEKGNLTKALSGKPVGTLIHRS
                     "
     gene            complement(11226..11447)
                     /locus_tag="PLA58_06830"
     CDS             complement(11226..11447)
                     /locus_tag="PLA58_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62546.1"
                     /translation="MAGKIFYRERLKIGVKEKKPRFKLVAVAGVDITFYGDHLRKREL
                     EEIAEAVGAELVLLKVQKKDKDEEVEVKD"
     gene            complement(11450..12187)
                     /locus_tag="PLA58_06835"
     CDS             complement(11450..12187)
                     /locus_tag="PLA58_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62547.1"
                     /translation="MLVYIGFDDTDLLGADTGTGKLARNFESLIPAPCSVLGVVRQQL
                     LVDPAIPYTSHNSSACVILENTDPALDDLLLGLAIEHLERSALPGSDPGLCFARADHP
                     GLAALQDFGLKCTRQIVTQREAIRAADSLRLSGHGGTCDGIIGAAAAVGLTASGWYGR
                     FIEYSGLRNFPDPVSVSALAQKGIRVVSLDRDAVTPCAEDEVHTKGWLRPRLWGSQAV
                     LPVNPSGPGRWESLGEKRNKKKPKKEE"
     gene            12622..>13747
                     /locus_tag="PLA58_06840"
     CDS             12622..>13747
                     /locus_tag="PLA58_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:13177..13179,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOR62548.1"
                     /translation="MNVSRRGFLKLSGAVAAVSGLGISLTPTAAHAKELKIKYAKETT
                     TICPYCSVGCSIIVSVRDGKVINTEGDPDSPINKGSLCSKGGSIYQMAVNENRLGKPL
                     YRAPFATEWKEVEWEWAFEKIAENVKKSRDASFKRTNAKGEVVNRTEGIASVGSAAMD
                     LEECFTYQKFLRGLGLVYIEHQARIUHSPTVPALAESFGRGAMTNHWVDFKNSDVILI
                     MGSNPASNHPVSWKWIQEAVNTRNAKIICVDPRFTQSASKAHLYAPLRSGTDIAFLGG
                     MIKYILENKLYFEEYVRNYTNASFLVNPAFKMPGDNKGVFSGLTNGKYEKDTWSYQTD
                     GAGVIRKDPTMEHPNCVFQLLKKHYSRYTPEIVSKICGTPV"
BASE COUNT         3092 a         3870 c         3908 g         2877 t
ORIGIN      
        1 caaaacggcg cctgcgaact gcaggacctg gtgtactggc tgcagatcga caagccccgc
       61 ttccccatcg agcatcacgg ctacgagatg gacaactcca acaccatgat tacccgcgac
      121 ctgaacaagt gcatcctctg cgggcgctgc atccgggcct gcaatgaaat tcaggtcaat
      181 gaggtgctgg atttcgccaa tcgcggatcc tacgcgaaag tcggcccggc ttttgatgcg
      241 gattacattg attcgtcctg cgttttctgc ggcgaatgcg cgcaggtctg ccccacgggc
      301 gccattacct tcaagcaggc gaaattcgcg ggccgtccct gggaactttc gaaaacccga
      361 accacctgcg cctactgcgg cgtcggctgc cagatcgacc tctacacaaa ggacaataaa
      421 atcgtcaaag tcatgggtaa tcgccaatac ggtccgccca atgaaggcag tctctgcgtc
      481 aagggccgtt tcggcatgga ctttatttcc catcccgacc ggatctccaa accgctgatt
      541 cgtcatcaga aaggcgaagc tttcaaagaa gccgcctggg acgaggcgta cgccttcatc
      601 gcacagcggt tggcggcggt taaaaaggaa tacggtccgg acagcattgc cgggctttct
      661 tccgcgcgct gcaccaacga ggaaaactac gtgttccaga aattcctgag ggggggcatc
      721 ggcaccaaca acattgatca ctgcgcgagg ctctgacact ccgtgacggt ggccggtctg
      781 gccgccacat tcggcagcgg agccatgacc aattcgatcg acgaattgga atctacggat
      841 ttgatcctgg ctacgggaac caatacgacg gaaaaccatc ccgtgatcgg cgctaaggtc
      901 aagcgggccg tccgtcggca tggaacgaag ctgattgtca tcgatccgcg ggaaatcgac
      961 ctcgtgaaat acgcggacat ctggctcagg caaaaaccgg gaacggatgt cgcggttttc
     1021 aacggcctga tgaacgtgat catcgcggaa ggtctttacg acaaggaata tgtcgcgggt
     1081 cgcacagaag gatttgaggc gctcaaagcg gtggttgcca aatacacacc tgagtccgtt
     1141 gaaaaaattt ccggcgtacc ggccgaagat ctgaaaaaag cggcccgcat gtacgcgcag
     1201 gccggccgcg cttccatcat ttacgcgatg ggcatcacgc agcacatcac cggcacggac
     1261 aacgtgaaga gcaccgccaa tctgtccatg ctgtgcggca acgtgggcat tgaaggaggc
     1321 ggcgtgaatc cgctgcgcgg ccagaacaac gttcagggcg cctgcgacat gggcgcgctg
     1381 cccaacgttt atccggccta ccagccggtt gccggcgagg acgtccgcaa gaaatttgag
     1441 gcggcctgga aagcggctct ttctcccaaa cccggcctga ccctgatgga aatcatggcg
     1501 gcggccggca gcggaaaaat caaagcactt tatgtcatgg gggaaaatcc ccttctttcc
     1561 gatccggacc tgcagcatgt cgcaaaagag ctgcagaaac tggatttgat gatcgtgcag
     1621 gatatttttc tgaccgagac ggcgcagatg gcggacgtgg ttctgccggc ctgtgccttt
     1681 gccgaaaagg aagggacctt taccaacacg gaacggcgcg tacagcgcgt tcgcaaagcg
     1741 ctggagccgc ccggagaggc cagggcggac tgggaaatca tctgcggaat ttccagccgg
     1801 atgggctatc cgatgacgta tgcgtccgcg caggaaattt ttgaagaaat caggacggtg
     1861 acgcccagct atgcgggcat tacctacgac cgcctggaaa aggaaggcct tcagtggccc
     1921 tgcccgaccg tcagtcattc ggggaccaaa ttcctgcaca aagacaagtt ctcgcggggg
     1981 ctgggactgt ttacggcgct ggagtatatc ccgcccaatg aactgccgga ccaggagtat
     2041 cccttcctgc tttcaacggg gcgtgtcctg tatcactacc acaccggcac gatgacgacg
     2101 cgcgccaggg gccccgtcga acgctgcccc gaaagcctgg tggaaattca tccggaagac
     2161 gcggggaaac tgggcattgc cgacggacag aaggtccggg tgacgtcccg gcgcggcacg
     2221 gtggaagtca aagcccgtat tacagaaaag agcgcgcccg gatcgatctt catgaacttc
     2281 catttcaccg aagcgcccgt gaatttcctg accaatccgg cgctggaccc caccggtaag
     2341 attccggaat acaaggtctg tgcggtaaaa ctggaagcag cctgattctc agcggccttc
     2401 gggccattgc tgcaacagag cgttcccgga aggacgacat ttccgggaac gctcgatccc
     2461 ttcttttcct ctcccgttta tttttattgc agatcatcat tattgctgtg ttttttcttc
     2521 gatttcgtcc acgtgcattt tgcgagttga catcagggaa atagttttgt atgaagagct
     2581 cactttatat gtcacttaag gcatatatca ttgatgtcat aagtgatttt caggagatgg
     2641 aaaaatgagc gacaccttta ttttaaacgg caaggagacg ccctatacgc cggggcaaat
     2701 gattctcgaa gcggcgaaag tcggcggcat tgagattcca accctctgtt acctgaaagg
     2761 ctcgacgccg accggcgcct gccgcatctg cctggtggaa gtcaaggggg cacgctcgct
     2821 gatggcggcc tgctccacgc cggtcacgcc ggggatggaa gtctttacgg aaaccgcact
     2881 cgtccatcag gccagaaagc tcaatgtgga actgctgctt tccagcggca cgcacaactg
     2941 tctcacctgc gaggccaacg gcaactgccg cctgcaggat ctggcctatt tttacaaaat
     3001 caccgatctg cggtttgagg aaaaagccgc gagcgatatt tatccgaagg aagaccggaa
     3061 tcctctgatc gttcgcgatt tcagccgctg catcctctgt ggccgctgcg ttcaggcctg
     3121 caatgaggtg atggtcaacc gggcgatctc tcaggggtac cgcggcaagg aaagcaagat
     3181 cgtcaccggc ggcgacctgc cctatcatga aagcgaatgc gttttctgcg ggcagtgcgt
     3241 ggaagtctgt ccggtcgggg cgctgaccga gaagaaagcc aagggcctgg gccgtccctg
     3301 ggaaatcaaa aaaatccgca cgacctgtcc ctactgcggc gttggctgcc agcagtggct
     3361 gcacgtcaag gacggcaaaa tcgtgaaggt gacggcggtg gaagacgctc agccgaacat
     3421 gggccgtttg tgcgtgaaag gccggttcgc ctacgatttc atttattcgg aggaacggct
     3481 caagacgccg ctgatccgcg aggcaaacgg tgaattccgg gaagcctcct gggatgaagc
     3541 gctggacctg gtggccgaaa aattcaagga aattatcaaa aaagacgggc cggacgccat
     3601 tgccggcgtg agctgcgccc gcagcatcaa cgaagattcc tatcagatgc agaaattctt
     3661 ccgcgcggtg atcggcacca acaacatcga tcactgcgcg cgcacctgac acgcccccac
     3721 cgtcgccggt ctggcgactt cattcggttc gggcgcgatg accaactctt tcgccgatta
     3781 cgcgaaggca aaaatgatca ttctggtggg caccaacatt accgaggcgc atccggtcgc
     3841 ttcgacgttc gtcaaagacg ctgtgctggc cggcgccaaa ctgatcgtga tcgatccgcg
     3901 gcgcacggac atggtggatt ttgccgagat gcacgtgccc ctgaaggtcg gctccgatgt
     3961 ggccctgatc aacggcgtga tgaacgtgct cattgaggaa aatctgtacg acaagaaata
     4021 tgtggattcc tgtacggaag gttttgaaga cctcaaaaag aaagtgatgg agtatccgcc
     4081 cgagcgggcc gcggaaatca gcggcattcc cgccgacacc atccgcgagc tggcgcgcaa
     4141 aatggcgtcc gtcaaaccgg ccatgctgat gtacacgctg ggcatcaccg agcacatctg
     4201 cggcgtgaac aatgtcatgt cctgcgccaa tctccagatg cttttgggca acgtcggcgt
     4261 tgaatgcggc ggcgtcaatc cgcagcgcgg ccagaacaac gttcagggcg cctgcgacat
     4321 gggcgcgctg cccaatgttt tccccggcta tcagcaggtg gccaatgcgg acgcccgggc
     4381 caagtttgcc aaagcctgga acgtcaaatc ccttccggat aaacccggca tgatgatgcc
     4441 gacgatgatg aaaaatctga ccaccggaaa agtcaaagcc ttttatgttt tcggtgaaaa
     4501 cctggccaat accgaaccgg atattcatca tgtcgagcgt gagctgcaat cggcggagtt
     4561 cctggtctgc caggatatct tcccgacgga aaccacccgg tttgcgcatg tcattctgcc
     4621 ggctgccgcc tggagcgaaa acgacggcac cttcgccagt tccgaacggc gcgtcaaccg
     4681 cgtgcgcgcg gccagtgtgc cgccggggga tgccagaccc aactggtgga tcttcaaaga
     4741 gctggccaaa cgtttcggcc acgagtggca atcctcaagc gctcaggaaa tctgggacaa
     4801 tgaaatatcc gtgctggcgc cttccatggc gggaatcaaa tacgcgcgtc tggaaaatga
     4861 cggtctgcag tggccctgtc ccaccgaaga tcatccgggc accccaacgc tgcacaaggg
     4921 cggaaaattc acccgtggtt tggggatgct gaaagccatc gactatattc cgcccgcgga
     4981 agttcccgat caggaatatc cgtttgtcct ctccaccgga agacgcctgt atcattacca
     5041 tacccggaca cagaccgggc ggtccgcggg catcaacgat ctgttgggcg aggagacggc
     5101 cgatatttcc acggccgatg ccgaggccaa aggcatttcc gacggtgaaa aaatctccgt
     5161 gaaatcccgc cgcggcgagg tggtcgtgaa ggcgcgcgtg acgaaggaag tgcctcccgg
     5221 cctggtctgg atggcgtttc atttccgcga agcctgcgcc aactggctga ccaacccggt
     5281 gtatgatccc gtaacgcaga cggcggagta taaggcctgc gcggtgagga tcgataaact
     5341 tagataacgg ccgaccaaaa acatcccgca gcaaaattgc gggatgtttt ttgtgtaaat
     5401 ctatgttatt ttagacatac ttgaaagaga gagaaagcgg ttgccatgaa aaaaaccatt
     5461 cttgttgtca tgatgttaag catccttgcc gcaccgtttc atgtttttgc ccaggataaa
     5521 ctttccgtcg cggttgcggc caactttatt tccgctttta aggagatcgc ggcggctttt
     5581 gaagagcaga ccggcgtaaa ggtggaagcg acattttctt cgacgggcaa cctttacagc
     5641 cagatcaaaa acggcgcgcc ttatgacgta tttctgtccg ccgacgagca gcgtcccgat
     5701 cttctgcaaa aagagggatg gacggacggg acgtttattt atgcgcgcgg acaggccatt
     5761 ctgtggtcgg ccgaccccaa tttctgtaaa gcgccaacct ggcaggaagc gctgaagaac
     5821 aacaggatta aaaagatctc gattgccaat cccgtcaccg ctccttacgg cgcggcggca
     5881 aaggcggccc tccaaaaagc cggactgtgg gatgcgctgc aggacaaact ggtcaacggc
     5941 caggatatcg cgcaggcctt ccagtacgcc agcacacagg cggtggatgc cgggttttgc
     6001 gcgctttccg ccgcggcaag cccgcagggg aaaaacggct gcttcttcag cattccggaa
     6061 gcgccggaca ttgtgcaggc cgggtgtatc ttgaaaaaat ccaaaaatca aaaatgcgcc
     6121 gaactgttga tggcctatct gctcggcgcc gacgccgcca aaacgaagaa gaaatacggt
     6181 taccggtaaa tatggattat cttgcgcttt atgtgacgct gaaactggcc ctggtgacga
     6241 cgcttgttct gatggtactg gccgcgccgg tggcctatct gctggcctac gcccgcttcc
     6301 ccggaaaatc gcttgtggag gcgctgattt atcttccgat ggctctgccg ccgaccgtga
     6361 tcggatttta tctgattatc gtcatgggac cccggggctg ggccggacgg gtctgggaag
     6421 ccgcgaccgg cggatccctt ttgttcactt tcctcggcat cactattgcg tccgtcgttt
     6481 attccgttcc gtttgccgtc cagccgatga aggcggcctt ccagaagatc gaccgccgcc
     6541 tgctggaaaa tgcttacatc ctcggcctgt cgaagcgcgc cgcctttttg cgcgtgatta
     6601 ttcccaattc ggttgccggc attgccgccg ccgcggtgct ggtttttctg cactcgatcg
     6661 gcgccttcgg cgtgctgttg atggtcggcg gcagcatccc cggagaaacc aaagtggcgt
     6721 ccatcgccat ttatgaagcc gtcgaaatga tgaactacga ggccgccgga ctgatcgcgc
     6781 tcagcttcat tccgatcagc tacgcctttt tattgctgat caacagactc aacgcgaggg
     6841 ccggcgcatg accctttccg cctcattccg caagagactg aaatatttcg atctggagat
     6901 ttctttcgcc tgccgggcgg ggaaaatgct ggtgatgatc ggcccgtccg gcggcggcaa
     6961 gaccacggtc atccggatgt tggcggggtt gacgatgccg gatgccggac gtgtggtatt
     7021 cggcgatgag gtctggttcg attccgcaag gaatattcat ctgaagcctc agaaacgaag
     7081 gctggggtat gtctttcagg actacagcct ttttccgcat cttaatttgt atgacaacgc
     7141 ggcgtttgcc gcggaaagca aaaaggaggt cgatgaactc ttcgagcttt tcaaaatcag
     7201 ccatttgaaa aaacgcaagc cgcacatggt ttccggcggc gagcgtcagc gctgcgcgat
     7261 ctgccaggcg ctggcccggc atccgcgcct gctcctgctc gatgagccgt tttccgcgct
     7321 ggatgtggtt acgcgccgcg gcttgcggga agagctgaaa atcctgaagg ggaggctgtt
     7381 ctgtcccgtt atttacgtga cgcacgacat ccatgaagcc ctgtttctgg ccgatgagat
     7441 tctgccggtt gtggacggaa agatcgaccg gggctggatg cagcgcacca ttacgcggga
     7501 gccgggcgag gggatatccg ccaggggagc gcgcgaaccg cgcctgtcgc tggtttactg
     7561 atcattacac aggaaaccgt catgacggtt tcctgtgggt tcaaggagca aaaataatca
     7621 tgaacatctg tacctattcc tacgaagagt atctgcatct ggtcaaatca tttcacggca
     7681 atctggcccc cggcctgatc atcggcggtt ttatcgtgga tcttgcgatg aagcatctgc
     7741 cggcggggga ttttttcgac gccgtctgcg aaacgccggt ctgtcttccc gatgccgtcc
     7801 agattctgac gccctgcacg atcggcaacg gctggctttc cgtcgttccc ttcggcaaat
     7861 ttgccgtcac gctgtatgaa aaatacagcg gcaggggcgt tcgagtctat ctcgatatgg
     7921 aaaaattgaa agcctggccg gaaatccacg actggtattt gaagaaaaag aaaaagagcg
     7981 agcagaatcc ggatgtcctt ctggcgcaaa tcaaagaggc ggggcatcaa ttgctgagca
     8041 tccagcaggt gcaggttgaa ccggaaaagg tccgccgcag gaaaatgggg ccggtggctg
     8101 tgtgtccggt ctgcggcgag gcctatccga cgcgcgacgg agacaaatgc cgcagctgcc
     8161 agggagaaac gccctacaag gatgttgtgg ctgtcgatgc gatcaaatca tgagataaag
     8221 aaatgagcct ccaacccgga acccttctga atcgtttgta tgagcggctt gaaccggatg
     8281 cgaaaaaccg gcggatcgcc gagctgcgca tcggcctgag ctacgtgggt gtgaggctgg
     8341 atcatggcgg gacgggcctt gccgccgtac tcccggacgc cgcctccgga ggctgcacgg
     8401 tcctgccggc tgccggaaaa cttgccggtt cgtccgcggc ggacctgctt gcctatcttc
     8461 cggagggcaa aaacgctgtc gagcgcgccg ttggtctggc ggccgccaac gctttgattg
     8521 cgtcgtcttg cgatgcgtcg gaggaccgcg aggcaacgac gtatttcggc ctgaaaccag
     8581 gggaaaaagt cgccatggtc ggtctttttt cgccgctggt cgaacgcatt cgcgccaccg
     8641 gcgccatcct caccgtcatt gaaaaaaatc ccgaacggct ggatcttctg ccgccggatc
     8701 aaaaacaaca ggcgcttgcg ggttgtgacg tggcgatcgt caccgccacg acgcttctca
     8761 atcatactct ggaggaaact cttgcggcgc tggcttctcc gcgcgtcgtg gccgtgatag
     8821 ggccttccac gccgcttctg ccggccgttt tctccggcac gcccgtcacc catcttggcg
     8881 gggccgttgt tgccgattcc acccgcgttt tgcaaatcat ctccgaaggc ggaggcacac
     8941 cggcgctgcg gccgtctttg cgtttcgtca atctgacgat cgggccctga agcgttgaaa
     9001 cggggcggtg tctattcacc cgcgtgttcg cgcgcgaccg ttttgccccg ttcttcatta
     9061 acgaaaagga cgatcacgac ggcaatcagg aaaaaggcgg aacagaccag aatcgcctgg
     9121 tgaagaccga acagcgtctg caacaggccc aatcccataa gccccgcaat ggccgacaga
     9181 cggctggtga ggctccacag accgaagaac tcgccggctt tggtgtccgg cgagaaaagc
     9241 cctaccatgg cgcggcacgc ggactgcgtc gatcccaggc ccaaccccgc aatcgatccg
     9301 atgatcagaa aaatatgctc ttcccggaga ggcgttccca gcagactgct cacccaagcg
     9361 gtcacgggag ccaccccgta aatcaggctc acggcagcca cccagatgat gagggtcagg
     9421 atgaaggtac gcttcgcccc ccatgtgtcc tgaagaatac cgaaaagaag agcgccgccg
     9481 gccgctgtga actgtgtgat gacaaacatc aggatctgtg tcttgccgga ccatcggatc
     9541 acctggtccc cgtaaataaa tgcaaaggag atcacgatgg aaaggcccgc gtacgcgaaa
     9601 aagaaggagg acagcagaac gatcaggtct ttgtagtccc gaatgtcccg taaagttctt
     9661 ttcaatcttt tcaagccgat cttcaggtaa gtttcacccc cgggcaaagc cctgggcgat
     9721 gtctgttctc tcacccagag aaacgtcgga atcgcggcga tgagaaaaaa caggcccgtg
     9781 gccggcccca ccagtcggag gttggaaaaa ttttccagcg tatagacgcc cgctcccagc
     9841 ccgaagatga cgatggctgt ggaggccagg ccgccgaagt aaccgagtcc ccaggcgtag
     9901 cccgaaatct tccccagatc ccgggaaggg cccaggcccg gaagaaagct ggaaataaac
     9961 gcttcgctgt aggaaaagcc gatgttggaa acaatgatga gcagcatgcc tatcagaatg
    10021 tctcccggtc cgacaaaata aagcgaagcg gtggcgagcg cgcaaagcca gcagctgccc
    10081 agcaggaatt tctttttgga gcccgtgtaa tccatgatcg cgcccagaag cggcgcggtc
    10141 aaaagcacga tcagatagct tatcgagatg gaggcgctcc acagcagatt gcccagccga
    10201 aattccggcc cgtctccgac gatgagccgg ggaaagatga tgcaaaacac cacggtgatg
    10261 atcacggttg tataggacga gttggcaaaa tcaaacatgg cccatccgaa cagctctttg
    10321 cgcgacgctc tttttatttc cgtcatggac ttttccttca tttgaatgcc gtgtggctca
    10381 ggaccggtgg ataagggttc ccacgggctt tccgctcagg gctttggtca gatttccctt
    10441 ttccagaccg ttgatgattt gaatcgtccg gatcacctcg ctgttttgca gaatttccag
    10501 gcagggcctt tcaatgatca gatcatccag gtcgcgcgcc agcagttcct tcacgctgat
    10561 ttcagggata aaagccgccc ggctgttttt cttgggatcg tccgtgtaga gtccgcgttc
    10621 gtccttgacg aaaatgcagc gccgggcgcc gatcagatcg gcggcgatca gcgtgccgac
    10681 gtcggtgcgg tgaatgggaa tgcgccgttt gggcgcgggc agggcgaaat aatcgtaggg
    10741 cggcatgccg tgcatgacgg gaatgcagcc ctgggaaaag taggtgtaga gtttggtgac
    10801 gtcgtcatgg ccgattttaa tgccccccca gggggtcagc agcatggcga ccaggagggc
    10861 gttctgctcg gagatggagc tgccgaattt ggcgatgacg cccgtcggca tgcccagttc
    10921 caggccgatg gcgtaaatgt ggcggctgcg tgtcccgccg ccggtcgtga gcagaatttt
    10981 gtgctttctg gagcaggcgg cgatttcttt gacgatcgcg ggcagcgcct gcgctccccg
    11041 gtcgcagatg gactgtccgc cgatcttgac cacgttgaca tcggggaaaa gcctcttctg
    11101 cggcgccacg gcgagaccgt ccataaaggt tcttcccacc agactttctc ccatcagcct
    11161 gcttttgaca tgcagccgct taccgtctcg ttctctgacc agggccatgc tcttttatcc
    11221 ttcctttaat ccttgacttc gacttcttca tccttatcct tcttttgaac tttgagcagc
    11281 accagctccg ctcctacggc ttcggcgatt tcttcgagct ccctcttccg caggtgatcg
    11341 ccgtagaaag tgatatccac accggccacc gccaccagtt taaaacgcgg ttttttttcc
    11401 ttgacgccga tcttcagcct ttcccgataa aaaatctttc cggccatttt cattcctcct
    11461 ttttcggttt tttcttgttt cttttttcac ccagactttc ccagcggccc ggcccggaag
    11521 gattcacggg caggacggcc tggcttcccc aaagcctcgg ccgaagccat cctttggtgt
    11581 gaacttcatc ttctgcgcaa ggcgtgacgg cgtcccggtc cagggaaacc acgcggatgc
    11641 ctttttgggc cagagcggat acggaaaccg gatcgggaaa atttcgcagt ccggagtatt
    11701 caatgaaccg gccgtaccag ccggaagcgg tcagccccac cgccgcggcc gcgccgatga
    11761 tgccgtcgca agtgccgcca tggccggaga ggcgcagcga atccgcagcc cgaatggctt
    11821 cgcgctgggt gacgatctgc cgggtgcatt tcaggccgaa atcctgaagc gcggcgagac
    11881 ccgggtggtc tgcgcgcgca aaacacagcc ccggatcgct tcccggcagc gccgagcgct
    11941 cgaggtgttc gatcgcaagc ccaagcagca gatcgtcaag cgcggggtcc gtgttctcaa
    12001 ggatgacgca cgcggagctg ttatgggacg tgtagggaat cgctggatcc accagaagct
    12061 gctggcgaac gactcccagg acgctgcacg gggccgggat caggctctcg aagttgcggg
    12121 ccagttttcc cgttccggtg tccgctccca aaagatccgt atcgtcaaag ccaatgtaaa
    12181 ccagcatttg cttctttacc tttcacggcc gccttggatg gcgggtttca ctataagtat
    12241 gccgctgatt tgtgtcaact caattcagcc gatccgggaa atcacggatg gcttcccgcg
    12301 gtttttttcc tttttgacta aaggtcatca acttcttccc ttcttaggac aaaactgcat
    12361 tgctacgatc tgacaggact tttttcttat gtcaaatatg acataaatag atgaaatgac
    12421 tcattttttt gcgttgacaa gcactcgcat tccatatata gaatttcccc gtgaaattat
    12481 agtatttcgg gtagcaggcg ctgactcttg ccccatgttt atcctggaga ctaaacccgc
    12541 tcaaacaact tttaatttgt tcaacaaggg agcgggaaat aaataagaca cctaaattct
    12601 ttaggaaagg agaaagaatt catgaacgtc agcagaagag gtttcttgaa actctccggc
    12661 gctgtcgccg cggtgagcgg tttggggatc agcctcacac caaccgccgc gcacgcaaag
    12721 gaattgaaga tcaaatacgc caaagaaacc accaccattt gtccttactg ctccgtagga
    12781 tgcagcatca ttgtgtccgt tcgcgacggc aaagtgatca acacagaggg agatcccgac
    12841 agccccatca acaaaggatc cctgtgcagc aagggtggtt cgatttacca gatggccgtc
    12901 aatgagaacc gtttgggcaa accgctgtac cgagctccgt tcgccacgga atggaaggaa
    12961 gtcgaatggg aatgggcttt tgagaagatc gcggaaaacg tcaagaaaag ccgcgacgcc
    13021 agtttcaaaa gaactaatgc caagggtgaa gtcgtcaacc gcacggaagg cattgcctcg
    13081 gtcgggtcag cggcgatgga ccttgaagaa tgcttcacgt atcaaaaatt cctgaggggg
    13141 ttgggcctgg tttacatcga acatcaggcc cgtatctgac acagcccgac tgtaccggct
    13201 ctggcagagt cgttcggacg cggcgcaatg accaatcact gggtcgattt taaaaacagt
    13261 gacgtaatcc ttatcatggg aagcaatccc gcttccaacc atcccgtttc ctggaaatgg
    13321 atccaggagg cggtcaatac gcggaatgca aaaatcatct gcgtggatcc gcgctttacc
    13381 cagtccgcct ccaaggcgca tctatacgcg cctcttcgtt cgggaacgga catcgctttc
    13441 ctgggcggca tgatcaagta cattctcgaa aacaagctgt attttgaaga gtatgtcaga
    13501 aattacacca atgcctcctt cctggtgaat ccggctttca aaatgcccgg cgacaacaag
    13561 ggggtttttt ccggccttac caacgggaag tatgaaaaag acacctggtc ctatcagacg
    13621 gacggagcgg gcgtgatccg caaagacccg acgatggagc atcccaactg tgtcttccag
    13681 cttttgaaaa agcactattc ccgctatacg ccggaaatcg tcagcaaaat ctgcgggacg
    13741 ccggtcg
//
LOCUS       DAOSRM010000059        13515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4645_length_13515_cov_117.705795, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000059 DAOSRM010000000
VERSION     DAOSRM010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13515
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4645_length_13515_cov_117.705795"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1330)
                     /locus_tag="PLA58_06845"
     CDS             complement(80..1330)
                     /locus_tag="PLA58_06845"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOR62549.1"
                     /translation="MKIGILTIGNELMNGRIADTNSAFIAREMNLQGWPVETMMSVGD
                     DFEIIADRLHYLLSFTDAVICTGGLGPTVDDITTAAVARAFDRQLIPDEKVLAYLKDI
                     FTRFNLRWTENNAKQALFPEGAEVIPNPVGTAAGFALPVGEKLVMVIPGVPSEMRRMV
                     PEGVISVLRRRFPQAVLQTVKQTIKTFGLGEAMVDQRLADVDFDALGVNVGFYPVFPE
                     NHLVLIARADSQEQAQKKIQKAVDQVTARLSDCIFSYGEETLEEKIAAAMTEKKLTIA
                     VAESCTGGLIADRLTDVSGSSVYFERGLVTYSNEAKIQLLGVPADILEKHGAVSEETA
                     RAMAEGVRKLAGTNLGLSSTGIAGPTGGTKEKPVGTVYIALTDGEKTVCRHYAYRWDR
                     RRNKLISSEAALMMLRNYLQETPS"
     gene            complement(1340..1804)
                     /locus_tag="PLA58_06850"
     CDS             complement(1340..1804)
                     /locus_tag="PLA58_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOR62550.1"
                     /translation="MATSQKDRWIRMLATGFGTGLSPVAPGTAGTLAGVLICLASWPL
                     PWLFRFFFVVAISAVSIYVAGEAEKHYGKTDDQRIVIDEIAGYQVAMLPVAVTGLHLL
                     TAFVLFRIFDIWKPFPLKHFQKFPGGWGVVADDLGAGVYAGILMLILVFLAP"
     gene            1972..2469
                     /gene="mobB"
                     /locus_tag="PLA58_06855"
     CDS             1972..2469
                     /gene="mobB"
                     /locus_tag="PLA58_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOR62551.1"
                     /translation="MIPIVSIVGKSNSGKTTLLEKIIADLVHRGYRVATIKHNRHGFN
                     IDHEGKDSYRHKKAGAHTTVVSSPHQLALVQDVDHDHSFEEIREKFISGADIILTEGF
                     KVNDYPKIEVFRSELKRELISKKEDGLVAVATNVSLDIDVPCLDLNDPKTVADFIEFH
                     FLKQS"
     gene            complement(2575..3486)
                     /locus_tag="PLA58_06860"
     CDS             complement(2575..3486)
                     /locus_tag="PLA58_06860"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOR62552.1"
                     /translation="MRKMTWLKAVVLAVLLVSPLALAAPVQAAARDIDPIVSTDWLQK
                     NLDNSNLVVVDVRKVEEYKAGHIPGAVNIVYGTWAVKKGDLLNELPAIDDLADAIGSA
                     GIDQNSLVVIVGKTDKVPDQFDMTRVAWTLKYLGVPNVAILNGGQNQWVKENKPVSQD
                     MTKSKEKSFTPSVNKDLFVDKASLAGKIGKAVILDNRAPAFFEGKEKLPFVPKAGRIK
                     GAVNLPVGQLFTPEGLYKDKAALEALAQKAAGSDLNKEIIAYCDTGKTCTGWAFIMTE
                     LLGYKDVKVYDGSSMEWLADPNAPSEP"
     gene            complement(3580..4272)
                     /locus_tag="PLA58_06865"
     CDS             complement(3580..4272)
                     /locus_tag="PLA58_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOR62553.1"
                     /translation="MSEKIFPEKNLVTRHNATELIEHWAIAISGLLLLITGIFELPVA
                     KRYYIITVPGLGWSADFITSLYIHYAAALVFTAAGIFHLIYHGLQSEKGLLPQKGDMK
                     ASVTVIKSLFGFGQEPPMHKYLPEQRWAYVGMALIIAMLIVSGLIKTYKNIYAPDMSL
                     AVVLTATWIHNICFVLFFLAFLAHMAAILLRPNWPMVRGIFTGKVRLNYARHRHALWI
                     TDLEKNEQSVDP"
     gene            complement(4265..5266)
                     /locus_tag="PLA58_06870"
     CDS             complement(4265..5266)
                     /locus_tag="PLA58_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOR62554.1"
                     /translation="MDAKNMSEITRRSFLRISAGAAALTGVHLPKTARAEAAGSLSTL
                     IDLSLCDGCANRKIPACIQACRSINRNKIPPVVDNIPEPWPRKTIEDWSKKQDVTNRL
                     TPYNYLYVHRAEVTVDGKRQTLFVPRRCMHCDNPACATICPFSANHKNQNGAVVINPN
                     QCFGGAKCRDVCPWEIPQRQSGVGIYLHVLPDFMGNGVMFKCDLCNDRLKEGKKPGCV
                     EACPRKAMLIGPRKDIEAEAEERARRMKGYIYGQKENGGTATLYVSPVSFELLNQTMT
                     RKPGQPDMKPNVERRMVGTDPLGKAVLAAPVLGLAAGLLGWFSRRKTKIKAEEQGHV"
     gene            complement(5277..5531)
                     /locus_tag="PLA58_06875"
     CDS             complement(5277..5531)
                     /locus_tag="PLA58_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOR62555.1"
                     /translation="MRIKVLNRLKRIEGQIRGLERMVEQEAPCADVLTQLYAATAAMK
                     KTGVVIIQANMRRCIEEAADDQDKSIEEFQKALTRFIGMS"
     gene            5698..6867
                     /locus_tag="PLA58_06880"
     CDS             5698..6867
                     /locus_tag="PLA58_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOR62556.1"
                     /translation="MNTYIDYHQRIKKIQDEMKKSDLDLFVGTRTVSLSYVAGAFVPW
                     RSAVVVSRDGYVGLISMLLDCERLKNESWLANISPYAPLPGMDLLDLIVHFVKQNKAQ
                     KGRIGVELGHSPRGNTGYLFATEFEFLKTALPAATFVNAVNIVDRACYIKEPGEIKLL
                     RQAAAIADAGINCVRHSLEIGMTETEIAGIGEMELRRLGSEYHWAVTGSSEIATGYRA
                     TYAMNGTTQPTQKMVQAGENIIVDLHPLYQLYPSDLAHNFILGKPSPEQQKLADAYLK
                     TAETIVRNFKAGNTVGDISKNVMDTVTELGYAQYTIPSFGHGLGVFGHEWYPAIVNND
                     EFRDVVLEENVVEVAFLAMTVPGVCGMRLECPVLVTKDGGEMLCKTPLALTVLEV"
     gene            7423..8319
                     /locus_tag="PLA58_06885"
     CDS             7423..8319
                     /locus_tag="PLA58_06885"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOR62557.1"
                     /translation="MKRNLLLRFVILASFIILLPTAGLARDIAPIVSTDWLLANLKNP
                     KLVILDIRRVEDYREGHIPGALNAFYGAWAYMKDGLYASLPEKDEIDDTISYMGIHFD
                     QWVVVTGCMDTPRLSYQSARVACTLQYAGIENVALLDGGMNKWIAEKKPLSKKIERRP
                     ASKFKGKYTSEKFADKHYIEERMGKLILLDLREREFFTGEKKMDCVPMRGHIPGAFNM
                     PTSCAFNEDRTFKTKEELRAIAEEAAGKDLNAAIVTYCDAGQCCPTWSYLLMEVLGYK
                     NVRLYVGSMQEWMQDPRAPVER"
     gene            8398..8574
                     /locus_tag="PLA58_06890"
     CDS             8398..8574
                     /locus_tag="PLA58_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62558.1"
                     /translation="MKNRRTFLLAFLAVWILALYGAAVFAATPNKCIQCHTNDALMKS
                     LHKPPVLPKSEGEG"
     gene            8608..9645
                     /locus_tag="PLA58_06895"
     CDS             8608..9645
                     /locus_tag="PLA58_06895"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOR62559.1"
                     /translation="MYKGYVVDKALLDKDPHFKAGCILCHAGNDGELSKEKAHLGMLK
                     KPSDNLRSCGVCHKKTAANYAKSLHYTTIGQRTGVMPRFSEAELKTFDEKVFEKSCRS
                     CHASCGDCHVKGAPVGGISIGLVAKHKFVKKDEGKTCAFCHGGRVYPEYTGDYGGAPD
                     VHYQKGMLCMDCHKKAEFHGDGTAYKSKNEVPQRPACKSCHPQGKEAKKETQVAHSLH
                     DGKVSCYGCHSGSAYRNCNDCHGGHAAAGPGMILGRSPRDRKVLTTLRLIPTVRDTFA
                     PAGIKMEHFDAVPNYWDTPAHNIKKRTERTRKCDVCHVDRKNFLTKETLIKNGAKANE
                     ELIFVPKAIAR"
     gene            complement(9769..9993)
                     /locus_tag="PLA58_06900"
     CDS             complement(9769..9993)
                     /locus_tag="PLA58_06900"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOR62560.1"
                     /translation="MTTHNDCVVLVESVSHALGAEKIIKGAGINCKLIHVPRHLSSDC
                     GICLRFRADDRERVEALLQGKLHFFDIKLL"
     gene            complement(9990..11165)
                     /locus_tag="PLA58_06905"
     CDS             complement(9990..11165)
                     /locus_tag="PLA58_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOR62561.1"
                     /translation="MEILYLDNAATSFPKPDTVMAAMIAYQQNIGANPGRSGHGRSVE
                     AGRIVFETREALARLFHIEDPLRIALTKNATESLNIVLQGGLKPGDSVITSGMEHNSV
                     MRPLRFLEGRGVELSVVPCSPRGELEPDDVRKALRKNTRMVVLTHASNVTGTILPVEA
                     VGALLRDRPEVLFCVDAAQTAGALPIDVEKMRIDLLAFTGHKSLFGPQGTGGLYIREG
                     LENKISPLMMGGTGSRSEFEQQPVFMPDRYESGTPNTIGIAGLGAGVSFVLEQTVGTV
                     RDQEERLISRFLSGLRDLRDDVIVYGPQDASRQTAVVSFNIRDVSASDAASYFEDNRG
                     ILCRPGLHCAPSAHRTIGTFPRGTIRFSFGFFNTQRDVDDACAALRELVKRKGNHRS"
     gene            complement(11421..11636)
                     /locus_tag="PLA58_06910"
     CDS             complement(11421..11636)
                     /locus_tag="PLA58_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOR62562.1"
                     /translation="MSTKKVDARGLSCPQPVVLVDRAIADGHTDLEILVDNEVARENV
                     SRLLKRRGLEAIISENVSEIMIRTAKT"
     gene            complement(11658..12737)
                     /gene="yedE"
                     /locus_tag="PLA58_06915"
     CDS             complement(11658..12737)
                     /gene="yedE"
                     /locus_tag="PLA58_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HOR62563.1"
                     /translation="MRNIFSTRAGIIIVGAIIGVGAALLQYFGNPPNMGICVACFERD
                     IAGALGLHRADVVQYLRPEIMGFVLGAFVAALLAGEYKPRGGSSPLIRFFLGIFAMMG
                     ALVFLGCPWRTLLRLAGGDGNALLGLAGLIAGIFIGVGFLKNGYSLGRSYAQKKAAGW
                     VFPALMIGLLLLLVFQVSFAPGGPIFFSAKGPGSQHAPILISLIAGLVIGGLAQRSRF
                     CTMGAFRDVILIRDFHLISGVAALLIFAFAANMLLGQFKPGFEGQPVAHTDHLWNFLG
                     MTLAGLAFVLAGGCPGRQLFLSGEGDMDAAIFATGMIVGAGIAHNFAIASSPKGTGAF
                     GPAAVVIGLLFCLVIGLTQRDKVSA"
     gene            12882..13394
                     /locus_tag="PLA58_06920"
     CDS             12882..13394
                     /locus_tag="PLA58_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62564.1"
                     /translation="MNWSALRLPRPLESAGSAEKIREALAASTILLWQEGNLRVPLLH
                     MSEPLAEALQRARLQRRIRFGFEDIAGRLAAEKKGIDSLRQKMTSQEQNRVSRLLLFS
                     GDGAQRLYRHIGQILIEHRRRLLGCRLDTDSKTLGKLLGAGSVGIKVILVEHKDAVSD
                     VLRALVDGRE"
BASE COUNT         3058 a         3793 c         3738 g         2926 t
ORIGIN      
        1 tagtcgcggg tcttcagacc cgcgatacac agcgcgcctg aaggcacgcg ctgcattttc
       61 cctttgtgac gatttgtcgt caagagggtg tctcctgtaa ataatttctc agcatcatca
      121 gcgccgcctc ggaggagatc agcttgtttc tcctgcggtc ccagcggtag gcgtaatgac
      181 ggcaaaccgt tttctctccg tcggtaagcg cgatgtaaac cgttccgacc ggcttttctt
      241 tcgtgccgcc ggtcggtccc gcaatgccgg tggaggaaag gcccagattt gtccccgcca
      301 gtttgcggac gccctcggcc attgcccgcg cggtttcttc gctcacggcg ccgtgctttt
      361 ccaggatgtc ggccggcacg cccagcagtt gaatttttgc ttcattgctg taggtcacga
      421 ggccgcgctc aaaataaacg gaactgccgg aaacgtcggt gaggcggtcg gcaatcaacc
      481 ctcccgtgca ggactccgcc acggcgatcg tcagtttctt ttccgtcatc gccgccgcga
      541 tcttttcctc cagggtttct tcgccgtagg aaaagatgca gtcggacaga cgggcggtta
      601 cctggtcaac ggccttttgt atttttttct gcgcctgctc ctgagaatcg gcgcgtgcga
      661 tcagcaccag atgattttcc ggaaagacgg ggtaaaagcc gacgttcacg ccgagggcgt
      721 caaaatcgac gtcggccagc ctttgatcca ccatggcctc cccgaggccg aaggttttga
      781 tcgtttgttt gaccgtctga aggacggcct gcggaaagcg gcggcgcaaa acggaaatga
      841 cgccttccgg caccatccgg cgcatttccg acggaacgcc gggaatgacc attaccagct
      901 tttccccgac cggcagcgca aatcccgctg cagtgccgac gggattggga atgacttcag
      961 cgccttcggg aaacagggcc tgcttggcgt tgttttccgt ccagcgcagg ttgaatcgcg
     1021 taaaaatatc cttgagatag gccaggactt tttcatccgg aatcagttgc cggtcgaacg
     1081 cccgcgccac agcggcggtg gtgatatcgt caaccgtcgg tcccagacct ccggtgcaga
     1141 tcacggcgtc cgtaaaagac aaaaggtaat gcagccggtc ggcgatgatt tcgaagtcgt
     1201 cgccgaccga catcatggtt tcaacaggcc agccctgcag attcatctcc cgcgcaataa
     1261 acgccgaatt tgtgtcggcg atgcgcccgt tcatcaattc gttgccgatg gtcaaaatgc
     1321 cgattttcat aaaaccgcct tatggtgcaa gaaaaacaag gatcaacatc agaattcccg
     1381 cgtaaacacc cgccccgaga tcatcggcca ccacgcccca gccgccggga aacttctgaa
     1441 aatgtttcag cggaaaaggc ttccagatgt caaaaatccg gaaaagaaca aaggccgtaa
     1501 gcagatgcag accggtgacg gccacaggca gcatggcaac ctggtagccg gcaatttcat
     1561 caatcacaat tcgctggtcg tcggtttttc cgtaatgctt ttccgcctct ccggcaacat
     1621 aaatagagac ggccgatatt gcaaccacaa agaaaaagcg aaaaagccag ggcaggggcc
     1681 aggaggcaag acagatcaaa acacccgcca gtgtgcccgc cgtccccggt gcgacggggg
     1741 aaaggcccgt cccgaagccg gttgccagca tcctgatcca tctgtccttt tgagaggttg
     1801 ccacggatgt cccttttcaa gcaggtttgt agaacgattg ttctatacta catgcttttg
     1861 ccggatgtca agcaatctta tgcccggacg cgcgcttttg gccgacattg ttggccaaag
     1921 ccggcataat gtggtatgta gccgccaaat aaaggaattt caggataaaa catgattccc
     1981 attgtttcca ttgtcggcaa atccaattcc ggcaagacaa cgcttctgga gaaaatcatt
     2041 gccgatttag tccatcgggg ctatcgagtc gccaccatca agcacaaccg ccacggattc
     2101 aacattgacc acgagggaaa ggacagctac cgtcacaaaa aagccggagc gcacaccacc
     2161 gtcgtttcat cgcctcatca gctcgcactg gttcaggacg tggaccatga tcactccttt
     2221 gaggaaattc gcgaaaaatt catttccggc gcggatatca ttctcaccga aggattcaag
     2281 gtcaatgact atccgaagat tgaggtgttc cgctccgagc tcaaaaggga gctgatcagc
     2341 aaaaaagaag acggcctggt tgccgtggcc acgaatgtga gcctggatat cgatgttccc
     2401 tgtctggacc tcaacgatcc caaaaccgtc gccgatttca ttgaatttca ttttctgaaa
     2461 cagtcgtaaa gctctccgtc tgtttcggcg ttttggcttt gccgaaaaaa agcccggcgg
     2521 tttgacggcc gggctttcgt ttccttcagg cgatgaagcg ttgtttatcc tgctctaggg
     2581 ttcggaagga gcgttgggat ccgccagcca ttccatcgag gagccgtcat acaccttgac
     2641 atccttgtat cccagcagtt ccgtcatgat aaaggcccag ccggtgcagg tcttgcccgt
     2701 atcacagtaa gcgatgattt ccttgttcag atcggaaccg gccgccttct gcgccagcgc
     2761 ttccagcgcc gccttgtctt tatacaggcc ttccggcgtg aacagctggc cgacgggcag
     2821 attgaccgct cccttaatgc ggcctgcttt gggaacaaag ggcagctttt cctttccttc
     2881 aaaaaaggcc ggggcgcggt tgtcgagaat gaccgccttg ccgattttac ccgcaagcga
     2941 agccttgtcc acgaacagat ccttgttgac ggacggcgtg aaagattttt ctttggactt
     3001 ggtcatgtcc tgtgagaccg gtttgttttc tttgacccac tgattctgtc cgccgttgag
     3061 gatggcgaca ttgggcacgc ccaggtattt cagcgtccag gcgacgcggg tcatgtcgaa
     3121 ctgatcggga accttgtccg ttttcccgac gatgaccacc agggagttct gatcgatgcc
     3181 cgcgcttccg atcgcgtcgg ccaggtcgtc gatcgccggc agttcgttga gcaggtcgcc
     3241 ctttttaacg gcccaggttc cgtaaacaat gttgacggcg cccggaatat ggcctgcctt
     3301 gtattcttcc acctttcgca catcgacgac aaccaggttg gaattgtcca aattcttctg
     3361 cagccagtcg gtggacacga tgggatcaat atccctcgcc gccgcctgga cgggagcggc
     3421 aagagccaac ggcgacacca gcaggaccgc caatacaacc gccttcaacc acgtcatttt
     3481 tctcataaag cactacctcc tttgtttaat tcattgtttg cttttcgcta ttttgaagcc
     3541 tttaacggcc ctcaaacctt tccgaagcgc agctcccggt tagggatcaa cggattgttc
     3601 gtttttttca agatcggtaa tccataaagc atgccggtgc cgggcatagt tcaggcggac
     3661 cttgccggtg aagatgcccc gcaccatcgg ccagttcgga cgcagaagaa ttgccgccat
     3721 gtgcgcgaga aatgccagaa aaaagagaac gaaacatata ttatgaatcc aggtggccgt
     3781 caggacgacc gcgagggaca tatccggcgc gtaaatgttc ttataggttt tgatcagacc
     3841 ggacacaatc agcatggcaa tgatcaaagc cattccgaca tacgcccagc gctgttccgg
     3901 caggtacttg tgcatcggcg gttcctgacc gaagccgaac aggctcttga tgaccgtgac
     3961 ggaagccttc atgtcgcctt tttgcggcag caaacctttt tcgctttgca gaccgtggta
     4021 aatcaggtga aagatgcccg cggccgtaaa aaccaatgcc gctgcgtagt gtatatataa
     4081 agaggtgatg aaatccgccg accagcccaa ccccggaacc gtgatgatgt aatagcgttt
     4141 ggccacgggg agttcaaaga tgccggtgat cagcagaaga aggccggaaa tggcgatcgc
     4201 ccagtgctcg atcaactcgg tcgcgttgtg gcgggtcaca agattttttt caggaaaaat
     4261 cttttcagac atggccttgt tcctccgcct ttatttttgt tttgcgtcgt gaaaaccatc
     4321 ccagcaatcc ggcggccagt cccagaacgg gcgcggcaag cacggccttt cccagcggat
     4381 cggtgccgac cattcgccgc tctacattcg gcttcatgtc cggctgcccg ggttttctgg
     4441 tcatggtttg attcaacagc tcaaacgata caggcgacac atagagcgtg gccgtgccgc
     4501 cgttttcctt ttgaccgtag atataacctt tcatccggcg ggcgcgctcc tccgcttccg
     4561 cctcgatatc ctttctgggg ccgatcagca tggctttgcg cgggcaggct tcgacgcatc
     4621 ccggtttttt tccttccttt aacctgtcgt tgcagaggtc gcatttaaac atgacgccgt
     4681 ttcccataaa gtccggcagg acgtgcaaat aaatgccgac gccggactgc cgctgcggaa
     4741 tttcccacgg gcagacgtcc cggcatttgg cgccgccgaa gcattggttg ggattgatga
     4801 ccactgcgcc gttttggttt ttatggttgg cggaaaacgg gcagatggtc gcacaggcgg
     4861 gattgtcgca gtgcatgcag cgcctcggga caaacagggt ttgccgcttg ccgtccacgg
     4921 tgacttccgc ccggtgcacg taaaggtagt tgtaaggagt cagacggttc gtgacgtcct
     4981 gctttttgga ccagtcttcg atggtttttc tcggccaggg ctccggaatg ttatccacga
     5041 caggggggat tttatttcgg ttgatgcttc ggcaggcctg aatgcaggcc ggtattttgc
     5101 ggttcgcgca tccgtcgcaa agactcaaat cgatcagcgt cgacagggat ccggccgctt
     5161 cggcccgagc ggtttttggc agatggactc ctgtcagagc tgcggctccg gccgaaatcc
     5221 gcaaaaaaga acgtcgggtg atttccgaca tatttttggc atccattcgc ttttccctag
     5281 gacatgccga tgaaccgcgt cagcgctttc tgaaattctt cgatgctttt gtcctgatca
     5341 tcggccgctt cctcgatgca gcgcctcata ttggcctgaa tgatcacgac gccggttttc
     5401 ttcatggcgg cggtggccgc gtagagctgg gtcaggacat cggcgcaggg cgcttcctgc
     5461 tcaaccatcc gctccagccc gcggatttgc ccctcgattc gctttaatcg gtttaatacc
     5521 ttgattctca tttcatccat catatacccc gtggggtata tataaacatt ccctttgctc
     5581 ctgtcaaatg gtttttgcga tgattttcgg tccgcttgac ttgccggggg caaccggcct
     5641 atactcttat tgcggcgaaa gctgatgttc atcagcggac aaagaaacgg aggttttatg
     5701 aatacgtaca tcgattatca tcagcgcatc aaaaagatac aggatgaaat gaaaaagtcc
     5761 gatctggatc ttttcgtggg cacgcgcacg gtcagcctga gctatgtggc gggggcgttc
     5821 gttccctgga ggagcgcggt tgtcgtatcc cgggacggat atgtcggttt gatttccatg
     5881 ctgctggact gcgagcggct gaaaaacgag tcctggcttg ccaatatttc tccctacgcg
     5941 cccctgcccg gtatggatct gctggatctg atcgttcatt tcgtcaaaca gaacaaggct
     6001 caaaaaggca gaatcggcgt ggagctcggc cactcccccc gcgggaatac aggctatctg
     6061 tttgccacgg aatttgaatt cctgaaaacc gccctgcctg ccgccacgtt tgtcaacgcg
     6121 gtcaacattg tggatcgggc ctgttacatc aaggaaccgg gcgaaatcaa gctgctgcgc
     6181 caggccgccg ccatcgcgga cgcagggatc aattgcgtca ggcattccct ggaaatcggc
     6241 atgacggaaa cggaaattgc cggcatcggc gaaatggagc tgaggcgcct gggcagcgaa
     6301 taccactggg ccgtcaccgg ctcgtcggag attgccacgg gataccgggc aacgtacgcc
     6361 atgaacggaa cgacgcagcc cacgcagaaa atggttcagg cgggcgaaaa tatcatcgtg
     6421 gatctgcacc cgctctatca actgtacccc tccgatcttg ctcacaattt cattttggga
     6481 aagccgtcgc ccgagcagca gaaactggcc gacgcctatc tcaaaacggc ggagaccatt
     6541 gtccgcaact tcaaggcggg aaataccgtc ggcgacatca gcaagaacgt gatggacacc
     6601 gtgaccgaac tgggttacgc gcagtacacg attccctcgt tcggccacgg cctgggtgtg
     6661 ttcggccatg aatggtatcc ggccatcgtc aacaatgatg aattccgcga tgtcgttctg
     6721 gaggagaatg tcgtggaagt agcctttctg gccatgaccg ttcccggcgt ctgcggcatg
     6781 cggctggagt gcccggtgct ggtcacgaag gacggcggcg agatgttgtg caaaacgccg
     6841 ctggcgctga cggtgctgga ggtttgatgc ccgttaaagg gaagaatgaa aagccccggc
     6901 ctttttcccg taaaggccgg ggctttcgct tgaaaaaagc ggctggaccg ttatcttgga
     6961 gacgatttct tttttctgtc cggagccgga caggaaggcc gcattcaggt ggatatgccg
     7021 aatttcggca acagccatgc ctggacaaaa acccagacgg ccacaaccag cagtatgatc
     7081 agcaaatcat tcatttttgt tttatcccgg atttgtggtt aacccccgca tttgtaaaga
     7141 ccatagcatc aggccggccg gggtgtcaat gctttgaatc ccgcgtaagc gggacgagcg
     7201 ttcattctcc gcggcttgct gcgaggtggt tgattcaaat gagctatgaa tcaacgccgg
     7261 ggataacttc agatggaagt tttgcagttt gggctgctag ccctgaaggg aacattgaac
     7321 tggctatttt gaacccggac atgatataat ttacgcgact ttaagacgaa aaggttttga
     7381 cgatacgctc attccgagcg ctggttgaca aggaggaaat ccatgaaaag gaaccttttg
     7441 cttcgattcg ttattcttgc ttcgttcatc attttgctcc cgaccgcggg actggcgagg
     7501 gacattgccc cgatcgtctc caccgactgg cttctggcca atctgaaaaa ccccaaactc
     7561 gttattctgg acatccgccg cgtggaagat taccgcgaag gccacattcc cggggcgctg
     7621 aatgcttttt acggcgcatg ggcctacatg aaggacggcc tgtatgcgtc gctgccggag
     7681 aaagacgaaa ttgacgacac gatcagctac atgggaatcc attttgacca atgggtggtt
     7741 gtcacgggat gtatggacac gccgcgtctc agttatcaga gcgcacgcgt cgcctgcaca
     7801 ctgcagtatg ccggcattga aaacgtcgcg cttttggacg gcggcatgaa caagtggatt
     7861 gcggagaaaa aacctttatc caaaaagatt gaaaggcgtc cggcgagtaa gtttaagggg
     7921 aaatacacct cggaaaaatt tgccgacaaa cactatatcg aagaaagaat gggcaaactc
     7981 attctcctgg atcttcggga acgcgaattt tttacagggg agaaaaagat ggactgtgtt
     8041 cccatgaggg gccacattcc cggcgcgttc aatatgccga cttcctgcgc ctttaacgag
     8101 gacaggacct ttaaaacgaa agaagaattg cgggccattg ccgaagaggc cgcgggcaag
     8161 gacctaaacg ccgcgattgt cacctactgc gacgcgggcc agtgctgccc gacctggtct
     8221 taccttctga tggaggtgct ggggtacaaa aacgtcaggc tctacgtcgg ttccatgcag
     8281 gagtggatgc aggaccctcg ggcgccggtg gagcgctgac gaacgctcca tgaatccgtt
     8341 tgacagccgg aaactgattt gttacatcta tctgcactct tcccttcagg aggcgttgtg
     8401 aaaaaccgac gaacattcct tcttgccttc cttgccgtgt ggattcttgc gctttatggc
     8461 gctgcggtct ttgccgctac ccccaataaa tgcattcagt gccatacgaa tgatgcgttg
     8521 atgaagtccc ttcacaagcc gcccgttttg cccaaaagcg agggcgaggg gtgagcgggt
     8581 gctcctccgc cagttcggcc ggaagaaatg tacaaaggat atgtggtcga taaggccctg
     8641 ctggacaaag atccccattt caaggcgggc tgcatcctgt gccatgccgg aaacgacggg
     8701 gaactcagca aggaaaaagc ccatttggga atgctgaaaa agccttcgga caatctgcgg
     8761 tcctgcgggg tctgccacaa aaaaaccgcg gccaactacg ccaagtcgct gcactacacc
     8821 acgatcggcc agcggacggg cgtcatgccc cggttctccg aagcggagtt gaagacattt
     8881 gacgagaagg tttttgaaaa atcctgccgg agctgtcacg cttcctgcgg cgactgccac
     8941 gtgaaaggcg cgccggtcgg cgggatcagc atcgggctgg tcgcgaagca caaattcgtt
     9001 aaaaaggacg agggcaaaac ctgcgcgttt tgccacggcg ggcgtgtcta tcccgaatac
     9061 acgggggatt acggcggcgc tcccgacgtg cattatcaga agggcatgct gtgcatggac
     9121 tgccacaaga aggcggagtt ccacggcgac ggcacggcct acaagtccaa aaacgaagtc
     9181 ccgcagcgcc cggcctgcaa gagttgccac ccgcagggca aggaagccaa aaaggaaacg
     9241 caggtggcgc acagtctgca tgacggcaaa gtgtcctgct acggctgcca ctccggctcc
     9301 gcctaccgca actgcaacga ttgccacggc ggccatgccg cggccggccc cggcatgatc
     9361 ctggggcgct ctccacggga taggaaagtg ctgacgacgc tgcgcttgat tcccacggtc
     9421 cgcgacacgt ttgctccggc gggcatcaaa atggagcatt tcgacgcggt tcccaactac
     9481 tgggatacgc ccgcgcacaa tatcaagaag cggacggaac gaacccgcaa atgcgacgtc
     9541 tgtcacgtgg atcgcaagaa tttcctgacc aaagagacgc tgatcaagaa cggcgccaaa
     9601 gccaatgaag aattgatttt cgtgcccaaa gccattgccc gctaggcggt gaatagctat
     9661 cagccgttca atcatcgcga cgacatttga tccttatggc agcatcatgg tcatctgatg
     9721 gttttgaacc atgaggtttt gaattccaca gagaaggcct tcaccatttc agagaagttt
     9781 gatatcgaaa aagtggagct tcccctgaag gagcgcttcc acccgttccc ggtcgtccgc
     9841 gcggaagcgc aggcagatgc cgcaatcgga gctgagatgg cgcggcacat gaatcaattt
     9901 gcagttgatg ccggcgcctt tgatgatttt ttccgcaccg agcgcgtgac tcacggattc
     9961 aacaaggacg acgcagtcgt tgtgcgttgt catgaccggt gatttccctt ccgttttaca
    10021 agttcccgca atgccgcgca ggcgtcatcc acgtcccgct gcgtattgaa aaacccgaaa
    10081 ctgaaacgga tcgttccccg cggaaacgtg ccgatcgtgc ggtgggccga cggcgcgcag
    10141 tgaagtcccg gccggcagag gatgccccgg ttgtcctcga agtaggaagc agcgtccgaa
    10201 gcggagacgt ctctgatgtt gaacgatacg acggcggttt gccgggaagc gtcttgcggg
    10261 ccgtaaacga tcacatcgtc ccgcaggtcc cggagtccgg aaaggaaacg ggagatcaga
    10321 cgttcttcct gatcacgaac cgtcccgacg gtttgttcca gaacaaagga gacgcccgca
    10381 cccagaccgg cgatcccgat cgtgttgggc gtgccgcttt cataccggtc gggcataaac
    10441 accggctgct gttcaaattc ggatcggctt cccgttccgc ccatcatcaa aggcgatatt
    10501 ttattttcca gtccctcgcg aatatagagc ccgcccgtgc cctgggggcc gaacagggat
    10561 ttatgcccgg tgaacgccag cagatcgatg cgcatttttt caacatcgat cggcagggcc
    10621 ccggctgtct gggcggcatc cacgcaaaac agcacctccg gtctgtcgcg cagaagcgcg
    10681 ccgaccgcct cgaccggcag aatcgttccc gtcacattcg aggcgtgcgt cagcacgacc
    10741 atgcgcgtgt ttttccgtaa agcttttcgc acatcgtccg gttcgagttc gccgcggggc
    10801 gaacagggga ccaccgacaa ttccacgccg cgcccttcca gaaaccgcag cggtcgcatg
    10861 acggaattgt gctccatgcc ggatgtgatc acggaatcgc ccggcttaag ccctccctga
    10921 aggacaatat tgagagattc cgtcgcgttt ttggtcagcg cgatccgcag agggtcttca
    10981 atatgaaaga ggcgcgccag agcctcccgg gtttcgaaaa cgatgcgccc cgcctcgacg
    11041 gaacggccgt ggccggagcg tcccggattg gcgccaatat tttgctgata agcgatcatc
    11101 gctgccataa cagtatccgg cttgggaaac gatgtggcgg cattgtcgag atacagaatt
    11161 tccatgaaaa cttttccttc tgcggccatt gccgataaaa gggatataga ataatgttgt
    11221 ccaaagatat ccttttcgcc tgcctgagcg ttttgtataa atccaaggct cgcttcaggc
    11281 aatgacaaaa ctcgccttcg gctcaaacag ttgtcaaagg ctgatctttc gcttcgccaa
    11341 gattgtatta caaaacgctc ttaacctgaa ggtcacaagc aatggccgct caaagagata
    11401 tctttgggca acggccattt ttatgttttg gccgttcgga tcatgatctc cgaaacattt
    11461 tcgctgatga ttgcctcgag gccgcgccgt ttgagcagcc gggaaacatt ttcacgcgcg
    11521 acttcattgt ccaccagaat ttccaaatcc gtatggccgt ccgcaatcgc ccggtcaacc
    11581 agaaccaccg gctgcgggca ggacagccct cgggcgtcaa ctttttttgt gctcatacct
    11641 gttctccttc ccgaaaatca agcggatact ttatcccgct gcgttaagcc gatgaccaga
    11701 caaaacaata acccgatgac gacggcggcc ggaccgaagg cgccggtgcc tttgggcgaa
    11761 ctggcaatgg caaagttgtg ggcgatgccc gcgccgacga tcatgccggt ggcgaaaatc
    11821 gcagcgtcca tatcgccctc tccggacaag aagagctgac gtccgggaca gccgccggcc
    11881 agaacaaacg ccagtccggc cagcgtcatg ccgaggaaat tccacaggtg atcggtatgg
    11941 gcgacgggct ggccctcaaa acctggcttg aactgtccca gcagcatatt ggccgcaaac
    12001 gcaaaaatca gaagcgccgc cacgccgctg atcagatgaa aatcccgaat cagaatcacg
    12061 tcgcgaaacg ctcccatggt gcaaaaccgg ctgcgctgcg ccagaccgcc gatgacgagc
    12121 ccggcgatca ggctgatgag gatcggggca tgctgcgatc ccggtccttt tgcgctgaag
    12181 aaaatcgggc cgcccggcgc aaaggaaacc tgaaacacca acaacagcaa caagccgatc
    12241 atcaaagccg gaaataccca gccggctgcc tttttttgcg cataggacct ccccaggcta
    12301 tagccgtttt tcaaaaaccc aacgccgata aaaatgccgg caatcagtcc cgcgagcccc
    12361 aggagggcgt tgccgtcgcc gccggccagc cgcagcagtg ttcgccaggg gcagcccaga
    12421 aacaccaatg cgcccatcat ggcaaaaatg ccgaggaaaa agcggatcag cggcgatgag
    12481 ccgccccggg gtttgtattc accggcgagc agcgcggcga caaaagcacc cagaacaaat
    12541 cccatgattt ccggccgtag atactgcacg acatccgcgc ggtgcagtcc cagcgcgccg
    12601 gcgatgtcgc gctcaaaaca ggcgacgcaa atccccatgt tgggcggatt gccgaaatac
    12661 tgcagcagtg ccgcgccgac gccgatgatt gcgccgacaa taataattcc cgcgcgggtt
    12721 gaaaaaatgt ttctcattac gaaacctcct ttgtaagatg cgaaatttct agcagaagcc
    12781 tcggtcatta tccaattcat aatgggaata tccggacggc tgttataacc gaaaggaatg
    12841 tgctacaacg ccaaaaaaga cgagattgaa tggggaatct catgaactgg tctgctcttc
    12901 gcctgccccg tccgctggag tcggcaggca gcgcggaaaa aatccgggaa gcccttgcgg
    12961 cctccaccat cctgctttgg caggagggca atttgagagt gcctctgctg cacatgagcg
    13021 agccgctggc cgaagcgctg caaagggcca ggctgcagcg gcgcatccgc ttcggttttg
    13081 aagacatcgc aggcagactc gcggcggaaa agaagggcat cgattccttg cggcaaaaga
    13141 tgacatcgca ggagcaaaac agggtttccc gcctgctgtt gttttccggt gacggggcac
    13201 agcgcctgta tcgccatatc gggcagatcc tgatcgagca ccggcgtcgc cttctgggtt
    13261 gcaggctgga cacggacagc aaaacgctgg gaaaattgct cggcgccgga agcgtcggca
    13321 ttaaagtgat tctggtcgag cacaaggacg ccgtgtcgga tgtcctgcgg gcgctggtgg
    13381 acggaaggga ataaccggtt ttttggccca acaaagtttc atgtcatcca cctctgcgat
    13441 gagcacagga cgcgggattt gccgcaaccg caccgcttag gcggctggcc gccggaccgg
    13501 agaggcctga ttttg
//
LOCUS       DAOSRM010000060        13468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4659_length_13468_cov_110.043838, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000060 DAOSRM010000000
VERSION     DAOSRM010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13468
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4659_length_13468_cov_110.043838"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /locus_tag="PLA58_06925"
     CDS             complement(<1..2177)
                     /locus_tag="PLA58_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR62565.1"
                     /translation="MKTTSPKTKASRKPAAPAGKTKPTGKAPRKPSPTTDLSQLADAL
                     IANSGNGIYIVQEGKFVYVSPLYQQLTGYAESELIGAPSLDHVHPDDRERVRQEAIHC
                     LKAPRREPYEYRFITKQKEILWVLENITSILFRGRRAALGNFMDITGHKRAEEALRYN
                     EEKYRLMIETIQDGYFEVDLNGKYTFVNDVICRQLQYSREELIGSDNRKHQNEANAKK
                     TYQAFLDAYSTGKPVPALELEVIRKDGSTQIVEVSISLIRDTEGQPIGFRGISRDITE
                     RKQAAQAVLESEALQRRLMEALPIGLIVVDPETRVIDHANATAAVMFGATVDQIVGHR
                     CHTFLCPAQEGACPVCDLGKDVDNSERWMVCADGRHRPVLKTVKKIKIHGSEKLLECF
                     VDITERKKIETALQASEDRFRSMVESLPDPYSEINLEGAITFVNEAFVHETGYSRDEL
                     MGKKFNMLLDESNARLAKRKYGEIYKTGRGVKNLELAWISKSGRVVLAEVSVSPIRRA
                     DGTMTGFQSVYRDITERRQMEIALSRAKEEAEVASNAKSEFLASMSHEIRTPLNAILG
                     MADLLTETPLNKEQQKFVQIFREAGENLLNIINDILDISKVEAGHIHLEHIPFDLGKL
                     VEQTGDIMSVRAHKKELELACRVAPGVPTKLMGDPTRLRQVLINFIGNAVKFTESGEV
                     MLDVKLTGPSAVHTEQNTVDLTFSVKDTGIGIPADKIHNIFER"
     gene            complement(2289..3269)
                     /locus_tag="PLA58_06930"
     CDS             complement(2289..3269)
                     /locus_tag="PLA58_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOR62566.1"
                     /translation="MKYIDHGKGGPASCMTLQDGPVPQIKAGEVLIEVDFAGVNRPDI
                     YQRMGAYPPPPDASPVLGLEVSGRIAAVADDVATWTIGDSVCALVPGGGYAEYCAAPA
                     LHCLPVPRGLSLAQASALPENYFTVWSNVFDRGALTPGENFLVHGGAGGIGYTAIQLA
                     KAFGAYVFTTVAGPDQIEAVKKLGADVALDYATQDWAREIENLVGRRGIDCILDFVGG
                     AYIQKNLKLLARNGRLVQIAFMQGAKVELDCLPILTQNLTFTGSMLRPRSLEEKASIA
                     RALYKNVWPLFDAGKLQVLISAVFELAEAAQAHTLMESAAHVGKIVLKVR"
     gene            3607..4677
                     /locus_tag="PLA58_06935"
     CDS             3607..4677
                     /locus_tag="PLA58_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR62567.1"
                     /translation="MDMKRDYYEDIELFKSGTVLVWTILFLLFLFTLPLYMPSYNIYL
                     INIILVNVILAVGLNILVGYTGQISLGHAGFFAVGAYGTSIIMMHLHVPFLPAIIIAA
                     LIAAFLGFIIGLPALRLEGPYLAIATLGFGLTIMHIIGRWEVFGGRMGITAPPLDLGI
                     PGSSWSLVLKGDVQIYYLILVIAILLVIGARNLMKTRVGRAFMSIRDDDIAAEVMGVN
                     LTIFKTMSFAVSAFYAGIAGGLYGFVVSFFDPFSFNMILSIIFLVMVVVGGLGSVLGA
                     IMGAVLITYLQYDLLKNVENLPYIGGALVAISKQWFTVIGLANFSGIALGLIMIAIVI
                     FEPLGMFGIWIRIKKYWKTWPF"
     gene            4700..5572
                     /locus_tag="PLA58_06940"
     CDS             4700..5572
                     /locus_tag="PLA58_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR62568.1"
                     /translation="MFEMLLIEGLAIGACYGLFGVAVAIIYKTSDVVNFAQGEMGMMA
                     TFVAYHIMTAYGWPFYLAFPATLLFAVLLGMAIEICFLRPAKEPTVLGLLILTLGAEM
                     LLMGLAGWKWGAEQKDFPFLLSVHKNVEIFPGFALNEWTIGVFVLTAVVMVLLFLFFR
                     YSKLGIAMRATQQNENAAKIMGIKVERVFSITWGMSSLIGALAAMFFAARSVLDPNFM
                     MEPFMRAFAAAVLGGLTSIPGVVIGGLILGIIENFFGYVWPEWKPIVAFVVIILIITV
                     RPSGLFAKHYVKKV"
     gene            5632..6084
                     /locus_tag="PLA58_06945"
     CDS             5632..6084
                     /locus_tag="PLA58_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62569.1"
                     /translation="MSGKKISIILCACAAALIAGLLIWDAPRNRVQLLLEKGAIERTA
                     HLYFKAEKEGNLKQAYALLAPSSAYRQAHTYDSFLKDVAGYPPVKIDAYKIVDIYRLR
                     DNDNRSAYPDVDKFVQVEVEMTFVNSGRNNVFNYSFTFLKEKGSWYKG"
     gene            6149..7336
                     /locus_tag="PLA58_06950"
     CDS             6149..7336
                     /locus_tag="PLA58_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR62570.1"
                     /translation="MKRALIAVMILCAVLMIPFAAGAGKPGFDDKEIRIAQWGPQTGP
                     AAPWGSVARGSDILFKIINDEGGIHGRKIKYFIRDDQYNPAQTKAVVKELVEREGIFA
                     FVGGVSAAAGMAVKDYLAANNVIWVGPATAINEYVFPVQPYLFGIYPLYDDEASILTK
                     YAAEKLKAKKIAFFYQNDAYGKSGLDGCKDRMKALKMQLVEEVSTEPGEKDLSSQILR
                     LKKSGADVVIMWVNPTSAVIALKTAATVGFKPQWMSSNTLSDYALMNKITGGLWEGVI
                     TGAFAEPPDANTPLLKKYREAAKKYAPEERWGTFYMAGILFAEPLVEALKRTGRNLST
                     EAVLKQLNSMKNFQGIGPPVTWTPAVHQGTDAIMIQKCGPNSSYILLQNWTANELATW
                     KKK"
     gene            7419..8192
                     /locus_tag="PLA58_06955"
     CDS             7419..8192
                     /locus_tag="PLA58_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR62571.1"
                     /translation="MAHFKVEHLSIAFGGIKAVNDVSFEVEHNKIFSIIGPNGSGKTT
                     IFNMISGIYKPNAGEVFLEGENLVGLRPDTIARKGVARTFQNIQLFGNATVMDNLMLG
                     RHIHMKTGILSGAFMWGRHSASAREEIRHRELIEHIVDFLDLQSLRDQFVSNLPYGKR
                     KLVELGRALALEPKVLLLDEPSAGMNTEEKDDLRIWIKDIQEDYRVTILLIEHDMNMV
                     MGISDRILAINQGVKIIEGVPADVQKHPDVIAAYLGEED"
     gene            8192..8965
                     /locus_tag="PLA58_06960"
     CDS             8192..8965
                     /locus_tag="PLA58_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR62572.1"
                     /translation="MLSVKNIETWYDLIRALHGISFEIQEGQIVALLGSNGAGKTTTL
                     KTIMRILDDDDQPEKGTIEFLGKRIDRMDTEEIVRLGISYVPEGREVFPELTVMENIQ
                     MGAYTRRDRKGIQEDIEGIFKQFPILSERRNQQAGHLSGGEQQMLAIGRALMSRPKLL
                     MLDEPSLGLSPLLTKEIFNIIQRINKQGVTILLVEQNVNMALKYSHYAFLMENGRIVR
                     ADKPEVLREDDDVKEFYLGIATETSVKGYKRYRRKVRFR"
     gene            8976..10793
                     /locus_tag="PLA58_06965"
     CDS             8976..10793
                     /locus_tag="PLA58_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR62573.1"
                     /translation="MKKEEIQTLPQGLKFMVQEQPDRIAMRMKDYGIWHDITWAQYYE
                     NVKKVAMGLHALGVGRGDHVAIVGENKPEWLFSAMGVMSLGGIFVGVYTTNPAAECEY
                     IVGHSESVVFFCEDEEQFDKALVFRSRTPHLKKCVVWDMEGLKHFDDPMLMSFDDLMA
                     LGEKTDRENPGLYEKIAAQGKGEETASIIYTSGTTGPPKGAMISHRNYLWIAQATEKI
                     TRMETKDETISFLPLNHVYEQIFDMMMHMWAGHIVNFTENTDTVMNDLRDVSPTLFHA
                     VPRIWEKYHSAIVLKMADATWLKRTVFNLAFKIGSKYNTVKLAKAPMPWYLYIGYQIA
                     YFCVFWKLKERLGFDRVRLGFSGAAPISHEILKFFQSIGIPIREGYGLTETTGITHIS
                     DAQNFKLGTVGRNLPGAKVQIAEDGEILVQHGGIFQGYFKEPEATAAALEGGWFHTGD
                     VGEIDQEGYLKITDRKKDLIITAGGKNIAPQYIENLLKFSPYITDAVVIGDRRKYVTA
                     LIVIDEENVVKFAQDHKVQYTTFASLTKTEEVIKLISDEVEKVNKQIARVENIRKFRL
                     LDKKLYTEDGEVTPTMKVKRKSIAEQYKDVIESMYREGE"
     gene            11058..12050
                     /locus_tag="PLA58_06970"
     CDS             11058..12050
                     /locus_tag="PLA58_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR62574.1"
                     /translation="MADMGLCRQLAEAVGAGESPLIPKIFEALVNDDEAKVMLLASPP
                     ATVEELAQKTGLAGEAITAMMDSLFHRGLIFKAAKGGEKKFYRVKSIPQMHDSTSLTP
                     GISRQVLDLWKEYMRKEWPGYGQMIMDFLPGPIMRVIPVNESIEPQSKILAYDDAVKI
                     VENAKTLSVTNCSCRVISGNCEKPLEVCMQVDRAAEYNIERGTGRAITPKEAVEILRK
                     CREEGLVHVVDNRQTVGHVICNCCDDCCLNWAIMKGPKKWVAPSRFAAFVDVDLCSGC
                     ETCIDRCFFEALSMQDDFAAVDPDRCLGCGVCTVVCPTDALKLKEVRSSDFVPA"
     gene            12105..12542
                     /locus_tag="PLA58_06975"
     CDS             12105..12542
                     /locus_tag="PLA58_06975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOR62575.1"
                     /translation="MSPIFHDELLVPKEAEDQNGHVNNVEYLRWMQDAAMRHSEASGC
                     TAATNAAGATWVVRTHKIVYLKPAFAGDRVVVCTWVASLRRVQSLRRYKIFRKDDRAL
                     LAHGETEWVFVDAQKGTLRSIPRDVKDLFEILPEGQEGNFFES"
     gene            12557..13021
                     /locus_tag="PLA58_06980"
     CDS             12557..13021
                     /locus_tag="PLA58_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12686..12688,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HOR62576.1"
                     /translation="MPRVELNTQAPDFTMNDFNGKSVSLSDYAGQKNVLVVFNRGFMU
                     PFCRAHMAQLRRDYDSFATLNTEILVAGPETPETFRDYWKKHDLPFVGLPDPEHRVLK
                     LYGQEVKLFKLGRLPAQMLIDRAGKVRFVHYGHSMADIPPNGEILDLIRSQL"
     gene            complement(13016..>13468)
                     /locus_tag="PLA58_06985"
     CDS             complement(13016..>13468)
                     /locus_tag="PLA58_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOR62577.1"
                     /translation="PFFQMPAVFAASALAGAVILIWMAAGMLRSLPTLRLSWEPHQSK
                     MNHPVVSGILMSVSNPYWIIWWATIGIGYIFYAWQYGFWGIAFFFAGHISADLAWYSF
                     IAGAVDRGRRLLTDRLYRRLIGLCAVFLIGFAGYFAYAGLVKLKDLLS"
BASE COUNT         2962 a         3629 c         3882 g         2995 t
ORIGIN      
        1 aaccgctcaa agatgttgtg gattttatcc gctggaatcc cgatgcccgt atccttcacg
       61 gaaaatgtca ggtccacggt attttgttcc gtgtggacgg cggacggacc ggtcaatttc
      121 acgtccagca tgacttctcc cgattcggtg aacttgacgg cgttgccgat gaagttgatg
      181 agcacctggc gcaggcgcgt gggatcgccc atcagtttgg tcggcactcc cggcgcgacc
      241 cggcaggcca gctccaactc ttttttatgg gcccggacgg acataatgtc cccggtctgc
      301 tccaccagct ttccaaggtc gaacggaatg tgctccagat ggatatgccc ggcctccacc
      361 ttggaaatat cgagaatgtc gttgataatg ttcagaaggt tttccccggc ctcccggaaa
      421 atctgcacga atttctgctg ttctttgttc agaggcgtct ccgtcaggag gtccgccatt
      481 cccaggatgg cgttcaaggg ggttcggatt tcgtggctca tgctggccag gaattcggac
      541 ttcgcgttgc tggccacttc cgcctcctcc ttggcccggg aaagcgcgat ttccatttgc
      601 cggcgctcgg tgatgtcgcg gtaaacggat tggaagccgg tcatggttcc gtcggcccgg
      661 cggatgggac tgacggacac ttcagccagc acgacgcggc cgcttttgga gatccatgcc
      721 aattcgagat tttttacgcc acgtcccgtt ttgtagattt cgccgtattt ccgtttggcc
      781 agccgggcat tgctttcgtc caggagcatg ttgaattttt tccccatcag ttcgtcgcgg
      841 ctgtatcccg tttcatgcac aaaggcctcg ttgacaaacg tgatggcccc ttccagattg
      901 atttctgaat aggggtcggg aagagattcc accatgcttc ggaagcgatc ttcgctggcc
      961 tgcagggccg tttctatttt cttgcgctcg gtgatgtcca caaagcattc cagcagcttt
     1021 tccgagccgt gaatttttat tttcttgacg gttttcagaa ccggacgatg gcggccgtcc
     1081 gcgcagacca tccatctttc ggaattgtcg acgtctttgc cgagatcgca gacgggacag
     1141 gcgccttcct gggccgggca cagaaaagta tggcaccggt gtccgacgat ttgatccacc
     1201 gtggcgccga acatgacggc ggcggttgcg ttggcatgat cgatgacgcg cgtttcggga
     1261 tcgacgacaa tcaagccgat cggaagcgct tccatcaggc ggcgctgcag agcctcgctt
     1321 tccagaacgg cctgcgcggc ctgcttgcgc tccgtaatat cccgggagat gccgcgaaac
     1381 ccgatgggtt gtccttcggt gtcccggatg agagatatcg acacttctac aatttgcgtc
     1441 gagccgtctt tgcggatcac ttccaattcc agcgccggga cgggcttgcc cgtcgagtaa
     1501 gcgtccaaaa acgcctggta agtttttttg gcgtttgctt cgttttgatg cttgcggttg
     1561 tccgatccga tcaattcttc gcgggagtat tgcagctgcc ggcagatcac atcattgaca
     1621 aaagtgtatt tgccgttcag atcgacctcg aaatagccat cctgaatggt ttcaatcatc
     1681 aagcggtatt tttcttcatt gtatcgaagg gcctcttccg cgcgcttgtg tccggtgatg
     1741 tccatgaaat tgcctagcgc ggcgcgcctg ccgcggaaca ggattgacgt gatgttttcc
     1801 agaacccaca ggatttcctt ttgcttggtg atgaaccggt attcgtaggg ttcgcggcgc
     1861 ggcgctttta aacaatgaat cgcttcctgg cggacccgct cccggtcgtc gggatgaaca
     1921 tgatccaggg aaggcgcgcc gatcagttcg ctttcagcgt atccggtcag ctgctggtac
     1981 agcggactga catagacaaa tttgccctcc tggacgatat agattccgtt tccggaattg
     2041 gcgatcaggg catcggccag ctgagacagg tccgtcgtcg gggaaggttt gcggggtgct
     2101 ttccctgtgg gtttggtttt gccggctgga gcagccggtt ttcttgaggc ttttgttttc
     2161 ggggaggttg ttttcattgg ttgccaccct ctgatcgaag aagagaattt tgatacgggg
     2221 cctcgattcc caattacctg aaaaaaaggt tggtgtaaag gccgcaaaat ggttgccggc
     2281 agccttgttc agcgaacttt caggacgatc ttgccgacat gagcggccga ttccatcaac
     2341 gtgtgggctt gcgcggcttc tgcaagctca aagacggcgg agatcaaaac ctgcagcttt
     2401 cccgcgtcaa agagcggcca gacgtttttg taaagggccc gggcgatgga ggccttttcc
     2461 tcgagggagc ggggacggag catcgagccg gtgaaggtca ggttttgggt caaaatcggc
     2521 aggcagtcca gctccacttt cgcgccttgc atgaaagcaa tctgtaccag ccggccgttt
     2581 ctggccagca gctttagatt cttttgaatg taggcgccgc ccacgaaatc caggatgcag
     2641 tcgatgcccc gcctgccgac cagattttcg atttcccggg cccagtcctg tgtcgcgtaa
     2701 tccagcgcaa catccgcccc cagttttttc accgcctcga tttgatcggg gccggccacg
     2761 gttgtaaaga cgtaggcgcc gaaggctttg gccagttgaa tggcggtata gccgatgcct
     2821 cccgcgccgc cgtgaaccag aaaattttca ccgggcgtga gcgcgccgcg gtcgaagaca
     2881 ttcgaccaga cggtgaaata gttttccgga agcgcagacg cttgtgccag ggaaagcccc
     2941 cgtggaaccg gcaggcaatg cagagcgggc gcggcgcagt attcggcata tccgccgccg
     3001 ggcacaagcg cgcaaaccga atctccgatc gtccatgtgg ccacatcgtc cgcgacggcg
     3061 gcgattcggc ccgagacttc gaggcccagc acaggcgacg cgtcgggcgg cggtggatac
     3121 gcccccatgc gttggtagat atccggacgg ttaacacccg cgaagtcgac ttcaatcagc
     3181 acttcgccgg ctttgatctg cggcacgggt ccatcctgca gggtcatgca ggaggcgggc
     3241 ccgccttttc cgtgatcgat atatttcatg gttcctccgc gttttccaaa atgctcgcgc
     3301 tttgaccacc ggtgattaac ggtttttcct cctgccgggc gggtgcaaga agatgtcttt
     3361 catgccgtcc gtcctgatca aaatgccgtc tgatcctagc acaccggaaa gaaaaaaaca
     3421 cgggccttgt tccggtcccg ccgggtatcg ccttaaattt tcttttgttt ctagaataat
     3481 tctctttgat catgaaattt tcttgacaga caaatttcgt ttctatatga ttcgccaccg
     3541 gtttacgtca aatgaagcag atgcatctaa ccctctagtt ggctacagta gtggcgtggt
     3601 gatcctatgg acatgaagcg cgattactat gaagacatcg agcttttcaa gagcggcacc
     3661 gtcctggtct ggacgatcct tttcctgctt ttcctcttta cgctgcctct ctacatgcct
     3721 tcatacaaca tctatctgat caacattatc ctggtgaacg tcatccttgc ggtgggcctg
     3781 aatattctgg tgggttacac cggccagata tccctgggac atgccggttt tttcgcggtg
     3841 ggcgcctacg gcacatcgat cattatgatg catttgcatg ttcctttttt gcccgccatc
     3901 ataatagctg ctctgatcgc ggcttttctg ggctttatca tcggtcttcc ggcgctgcgg
     3961 ctcgaagggc cctatctggc gattgccaca ctgggcttcg gccttaccat catgcatatc
     4021 atcggtcgat gggaagtttt cggcggcaga atgggcatca ccgcgccgcc tcttgacctc
     4081 ggaattcccg gttcctcctg gagcctggtg ctcaagggag acgtgcagat ctactacctc
     4141 atcctggtga tcgcgatcct gctcgtcatc ggggcgcgca atcttatgaa gacaagggtg
     4201 gggcgcgcct tcatgtccat tcgcgacgac gacatcgccg ccgaggtcat gggcgtgaac
     4261 ctgacgattt tcaaaaccat gtcctttgcc gtcagcgctt tttacgccgg catcgcggga
     4321 ggcctttacg gatttgtcgt cagcttcttc gatccctttt cctttaatat gattctgtcc
     4381 attatcttcc tggtgatggt ggtggtcggc ggtctgggtt ccgtactggg ggcgatcatg
     4441 ggcgccgtcc tgattacgta tctgcagtac gaccttttga aaaacgtgga aaacctgccc
     4501 tatatcggcg gggccttggt ggccatctcc aagcagtggt ttaccgtgat tggactggcc
     4561 aacttctccg gcatcgcttt ggggctcatc atgatcgcca ttgtgatttt tgagccgctg
     4621 ggaatgttcg gcatctggat ccggattaaa aaatattgga agacctggcc gttttaaaag
     4681 gccaaaaggg agtcttggca tgtttgagat gctgctgatt gaaggtctgg ccattggagc
     4741 ctgctacggt cttttcggcg tcgccgtcgc cattatctac aagacgtcgg atgtggtgaa
     4801 cttcgcgcag ggtgaaatgg gcatgatggc cacgtttgtc gcctaccata tcatgaccgc
     4861 ttacggctgg cctttctacc ttgcttttcc cgcaaccctt ttgtttgccg tcctgctggg
     4921 catggccatc gaaatctgtt ttttgcggcc cgccaaggaa ccgaccgttc tgggcctgct
     4981 gattctgacc ctcggcgcgg agatgctgct gatggggttg gccggctgga aatggggcgc
     5041 ggagcagaag gactttccgt ttcttctttc cgttcacaag aatgtcgaaa tttttccggg
     5101 cttcgccctg aacgaatgga ccatcggtgt tttcgtgctg acggcggtgg tcatggtgct
     5161 gctgtttttg tttttccgtt attccaaact gggcattgcc atgcgggcga cgcagcagaa
     5221 tgaaaacgcc gccaagatca tgggcatcaa ggtggaaagg gtattctcca tcacctgggg
     5281 catgagctct ctcatcggcg cgctggcggc catgtttttc gcggcccgtt ccgtgctgga
     5341 ccccaatttc atgatggagc ccttcatgcg cgccttcgcc gcggctgttc tgggagggct
     5401 caccagcatt ccgggcgttg tcatcggcgg cctgatcctg ggcatcatcg agaacttttt
     5461 tggttatgtc tggccggagt ggaagccgat tgtcgccttt gtggtcatta ttctgattat
     5521 taccgttcgg cccagcgggc tgttcgccaa gcattatgtg aaaaaagtct agcgtgtttt
     5581 gcaggccgcc cgccatcgac cgggatcggc tgaagcatcc gggagctttt catgagcggg
     5641 aaaaaaatat ccatcatcct ttgcgcctgc gcggcggcgc tgatcgccgg gctgctgatc
     5701 tgggatgctc cccgcaaccg tgtgcagctg ctgttggaaa aaggagcgat tgaaaggacc
     5761 gctcatctgt acttcaaggc ggaaaaagaa gggaatctga aacaggcata cgcgttattg
     5821 gccccttcgt ccgcttaccg gcaggcgcac acctacgatt cgtttttaaa ggatgtagcc
     5881 ggttacccgc ccgtgaagat cgatgcctat aagattgtcg acatctatcg cctgcgggat
     5941 aacgacaatc gcagcgccta tcctgatgtg gataaatttg ttcaggtgga agtggaaatg
     6001 acctttgtca attcgggcag gaataatgtt tttaattaca gcttcacttt tctgaaggaa
     6061 aaaggaagct ggtacaaggg atgaaaccaa aagggtttga agggaagagc acaatcaaca
     6121 caccatacga tgaacaaaag gagggattat gaaaagggca ctgattgcag tcatgatttt
     6181 atgcgcggtc ctgatgattc cgtttgcggc cggcgcgggc aaaccgggat ttgacgacaa
     6241 ggaaatccgg attgcgcagt gggggcctca aacgggacct gccgcgccct ggggttcggt
     6301 tgcccggggc agcgacattc tgtttaaaat tatcaacgac gaaggcggca ttcatggacg
     6361 caagatcaaa tacttcatcc gtgacgatca gtataatccc gcccagacca aggccgtggt
     6421 caaagagctg gtcgaacggg agggaatttt tgcctttgtc ggcggggtga gcgcggcggc
     6481 cggcatggcg gtcaaggatt acctggcggc caacaacgtg atctgggtcg gtccggccac
     6541 ggccatcaat gagtatgttt tccccgtgca gccctatctt ttcgggattt accccctgta
     6601 cgatgacgag gccagcattc tgaccaaata tgcggcggag aaactgaaag ccaaaaaaat
     6661 cgcctttttc tatcagaatg acgcctacgg caaaagcggt ctggatggat gcaaagacag
     6721 aatgaaagcc ctcaagatgc agctggtgga agaggtttcc acggaaccgg gcgaaaaaga
     6781 tctgtcctct cagatcctgc gcctgaaaaa atccggggcc gatgtggtca tcatgtgggt
     6841 caatcccacc tccgccgtga tcgcgctcaa gacggccgca accgtcggct tcaagccgca
     6901 gtggatgtct tccaatacgc tttccgacta tgcgctgatg aacaagatca ccggcggact
     6961 ctgggaaggg gtgatcaccg gagcgtttgc cgagcctccc gatgccaata cgccgcttct
     7021 gaaaaaatac cgggaagccg ccaaaaagta tgccccggaa gaacgctggg gcactttcta
     7081 catggcgggc attctctttg cggaaccgct ggttgaagcc ctcaaaagaa ccggccggaa
     7141 cctcagcacg gaggcggtgc tgaagcagct caactcgatg aaaaactttc agggaatcgg
     7201 ccctccggtg acctggacgc ccgcggtgca tcagggaacg gacgccatta tgattcagaa
     7261 gtgcggcccc aattcttcct acattttgtt gcagaactgg accgccaacg agttggcgac
     7321 ctggaagaag aaataattca agacgcccgg gagaggcggt ttgcgccgcc tctcccgcga
     7381 cgataaccac cggttgagac ggtcacgggt taaaggctat ggcgcacttt aaagtggaac
     7441 acctgagcat cgctttcggc ggcatcaagg cagtcaatga tgtcagtttt gaagtcgagc
     7501 acaacaaaat cttttcgatt atcggaccga acggcagcgg caaaaccacg atcttcaata
     7561 tgatcagcgg catttacaag cccaatgccg gcgaggtctt tctggaaggc gagaatctgg
     7621 tcggcctgcg tccggatacg atcgcccgca agggcgtggc gcggacattt cagaacatcc
     7681 agctgttcgg caacgccacc gtcatggaca acctgatgct ggggcggcac atccacatga
     7741 aaacggggat tctcagcggc gctttcatgt gggggagaca ttcggcgagc gcgagggaag
     7801 aaatccggca tcgagaactt atcgaacaca tcgtggattt tctggatctg cagtccctgc
     7861 gcgaccagtt tgtgtccaac ctgccttacg gcaagcgcaa actggtggag ctgggacggg
     7921 cgctggcgct ggagcccaag gtgctgctgc tggatgaacc gtccgccggc atgaacacgg
     7981 aagagaagga cgatttgcgc atctggatca aggacatcca ggaagactac cgggtgacga
     8041 ttctgctcat cgagcatgac atgaacatgg tgatgggcat ttccgacaga attctggcca
     8101 tcaaccaggg cgtgaaaatt atcgagggag tgcccgcgga cgtgcagaag cacccggacg
     8161 tgattgccgc ctatctggga gaggaggact aatgctctcg gtcaaaaata ttgaaacctg
     8221 gtacgatctg atccgggcgc tgcacggcat ctcctttgaa attcaggagg gtcagattgt
     8281 ggcgcttctg ggcagcaacg gcgcgggcaa aaccacgacg ctcaagacga tcatgcggat
     8341 tctggacgac gacgaccagc cggaaaaagg cacgattgag tttctgggca agcgcatcga
     8401 ccgcatggac acggaggaaa tcgtccgcct gggcatcagc tacgtgccgg agggccgcga
     8461 ggtctttccc gagctgacgg tcatggaaaa cattcagatg ggcgcttaca cgcgccgcga
     8521 ccgcaaggga attcaagaag acattgaagg cattttcaag cagtttccca ttttgagcga
     8581 gcgcaggaac cagcaggccg gtcatctgag cggcggggaa cagcagatgc tggcgatcgg
     8641 ccgcgcgctg atgagccgtc ccaagctgct gatgctcgac gagccgtcgc tgggcctgtc
     8701 gcctctgttg accaaggaaa ttttcaacat catccagcgc atcaacaagc agggcgtgac
     8761 catcctgctg gtcgagcaga acgtgaacat ggcgctgaaa tattcgcatt acgcttttct
     8821 gatggaaaac ggccggatcg ttcgggccga caaaccggaa gtgctgcgcg aggatgacga
     8881 cgtgaaggag ttttatctgg gcattgccac ggagacctcc gtgaaaggct acaaacgtta
     8941 ccgccggaaa gtgcgtttcc gctaaggaag aaactatgaa aaaagaggag attcaaaccc
     9001 tgccgcaggg cctcaagttt atggtgcagg agcagccgga ccggattgcc atgcgcatga
     9061 aggattacgg aatctggcac gacattacct gggcccagta ttatgaaaac gtgaaaaaag
     9121 tcgccatggg actgcacgcc ctgggcgtcg ggcgaggcga ccatgtggcg attgtcggcg
     9181 agaacaagcc cgaatggctt ttctcggcca tgggcgtcat gtcgctggga ggaatcttcg
     9241 tcggcgttta cacaaccaac ccggccgcgg aatgtgaata tatcgtcggc cattccgagt
     9301 ccgtcgtctt cttctgtgag gacgaggaac agttcgacaa ggcgctggtt ttccgctccc
     9361 gaacgccgca tctcaaaaaa tgcgtggttt gggatatgga gggcctgaaa catttcgatg
     9421 atcccatgtt gatgagtttt gacgatctga tggcgctggg agaaaagacg gaccgggaga
     9481 atcccggtct gtacgagaaa atcgcggcgc aggggaaagg cgaagaaacc gcctccatca
     9541 tctatacctc cggcaccacc ggccccccga agggcgcgat gatcagccat cggaattatc
     9601 tgtggatcgc gcaggcgacg gaaaaaatca cccggatgga aacgaaggac gagaccattt
     9661 ccttcctgcc gctcaaccat gtctatgaac agatctttga tatgatgatg cacatgtggg
     9721 cgggccatat tgtgaacttc acggaaaata cggacacggt gatgaacgat ctgcgcgacg
     9781 tgtcgcccac gctttttcac gccgttcccc gcatctggga aaaataccac tccgccatcg
     9841 tgctcaagat ggccgacgcg acctggctga aacgaacggt cttcaacctc gccttcaaaa
     9901 tcggctcaaa gtacaacacc gttaaactgg cgaaagcccc catgccctgg tatctgtaca
     9961 tcggctatca gattgcgtat ttctgcgttt tctggaagct caaggagcgg ctcggctttg
    10021 accgcgtccg cctcggattt tccggcgcgg cgcccatctc gcacgagatt ctgaagtttt
    10081 tccagagcat cggcattccc atccgcgaag gctacgggct gaccgagacc acgggcatca
    10141 cgcacatctc cgacgcgcaa aacttcaagc tggggacggt ggggcggaat ctgccgggcg
    10201 ccaaggtgca aatcgccgaa gacggcgaga ttctggttca gcacgggggc attttccagg
    10261 gatacttcaa ggaacccgaa gccacggccg cggcgctgga gggcggctgg ttccacaccg
    10321 gcgacgtcgg ggaaatcgat caggaaggct atctcaagat caccgaccgc aaaaaagacc
    10381 tgatcatcac ggcgggcggg aaaaacatcg cgccgcagta cattgaaaac ctgctgaaat
    10441 tctctccgta catcacggac gcggtggtca tcggcgaccg gcgcaagtat gtcacggcgc
    10501 tcatcgtcat cgacgaggaa aacgtcgtga aattcgcgca ggaccacaag gtgcagtaca
    10561 cgacgtttgc cagtctgacc aagaccgaag aggtcatcaa gctgatttcg gacgaggtgg
    10621 agaaggtcaa taagcagatc gcccgcgtgg aaaacatccg caagttccgc ctgctggaca
    10681 agaagctcta cacggaagac ggagaagtca ccccgaccat gaaggtcaag cgcaaatcca
    10741 tcgccgagca gtacaaggac gtcatcgagt ccatgtaccg ggaaggcgaa tgaccgccgc
    10801 ccttccgtct gccgcaattt cgaaccgtcc tggaaagtcg gagaagaaat ccgcgcagcg
    10861 gcaaggtccg gggccgaaag cggcctcaag cccgctggtc ctgattttcc gcatctgccc
    10921 aaagcgccgg ccgtacgctc atcctttcag gccggtttct ttttttgtgt ccaagattcc
    10981 ccctggtgtt tgttcttgac aggaaagatt tatttcccga tactttctcc aattcctgga
    11041 aacggaggca atgacgcatg gcggacatgg gattgtgcag acaactggcc gaagccgtgg
    11101 gggcgggcga atccccgttg attccaaaaa tatttgaggc gctggtcaat gacgacgaag
    11161 caaaggtgat gcttctggcc tcaccgcccg ccaccgtgga agaactggcg caaaagaccg
    11221 gattggccgg ggaggccatc acggcgatga tggattccct gtttcaccgg ggattgatct
    11281 ttaaagcggc aaagggcggc gaaaagaaat tttatcgcgt gaaaagcatt ccgcagatgc
    11341 atgattccac cagcctgacg cctggaattt ccaggcaggt gctggacctc tggaaggagt
    11401 acatgcggaa ggaatggccc ggttacggtc agatgatcat ggattttctc cccgggccca
    11461 tcatgcgggt gattcccgtc aatgaaagta tcgagccgca atccaaaatt ctggcctacg
    11521 atgacgccgt gaagattgtc gagaacgcca aaacgctttc cgttaccaat tgctcctgcc
    11581 gtgtgatctc cggcaattgt gaaaagcctt tggaagtctg catgcaggtg gaccgcgccg
    11641 ccgaatacaa tatcgaacga ggcaccggcc gggccataac tcccaaagaa gccgtggaaa
    11701 tcctgagaaa gtgccgcgag gaaggtcttg tgcatgtggt ggacaaccgc cagacggtgg
    11761 gccacgtcat ctgcaactgc tgcgacgact gctgcctgaa ctgggcaatt atgaagggac
    11821 cgaaaaaatg ggtggcgccc agccgtttcg cggcctttgt cgatgtcgat ctttgcagcg
    11881 gatgcgaaac gtgcatcgac cggtgctttt tcgaagctct ctccatgcag gatgattttg
    11941 ccgccgtcga tccggatcgg tgcctggggt gcggggtctg caccgtcgtc tgcccgacgg
    12001 acgccctgaa gctcaaggag gtgcggtcat ccgattttgt tccggcctga ggcggagaag
    12061 gataacaaag gaccggctgt tgatgtgagg aaaacctgac ccttatgagt ccaatttttc
    12121 atgatgagct tttggtgccc aaagaagcgg aagatcaaaa cgggcacgtc aacaatgtgg
    12181 aatatctgcg ctggatgcag gacgcggcca tgcggcattc cgaagcgtcc ggctgcaccg
    12241 ccgccacaaa cgccgccggc gccacctggg tggtgcgcac gcataaaatc gtgtacttaa
    12301 aaccggcgtt cgccggcgac cgggtggtgg tctgcacatg ggtggcgagc ctccgccggg
    12361 ttcaatcgct gcgcaggtat aaaattttcc ggaaggacga ccgggcgctg ctggcccacg
    12421 gagaaacgga atgggttttt gtggacgcgc aaaaaggaac gctgcgttcc attccaaggg
    12481 acgttaaaga tttgtttgaa atcctgccgg aagggcagga gggaaatttt ttcgaatcgt
    12541 aagggaggat tgcacgatgc ccagagtgga gctgaacacc caggcgcccg attttacgat
    12601 gaacgatttc aacggcaaaa gcgtgtccct gtccgattac gccggacaaa aaaacgttct
    12661 ggtcgttttc aaccgcggct ttatgtgacc gttctgccgc gcgcacatgg cgcagttgcg
    12721 ccgggattac gattcgttcg cgaccctcaa cacggaaatc cttgttgcgg gccctgaaac
    12781 acccgagacg tttcgggact actggaaaaa acatgacctg ccgtttgtcg gccttcccga
    12841 tccggagcac cgcgttctga aactgtacgg ccaggaagtg aaactcttca agctgggccg
    12901 cctgcccgcg caaatgctga tcgaccgcgc gggaaaggtc cgcttcgtgc actatggcca
    12961 ctccatggcg gacattcctc ccaacgggga gattctggat ttgatccggt cgcagttatg
    13021 acagaaggtc tttgagtttg acgaggcccg cgtaggcgaa ataaccggca aaaccgatca
    13081 ggaaaacggc gcaaagaccg atcagccggc ggtagagccg gtcggtcagc aggcgcctgc
    13141 cccggtccac cgcgccggcg atgaaagaat accaggccag atccgccgaa atgtgaccgg
    13201 caaagaaaaa agcaattccc caaaaaccat actgccaggc gtaaaaaata tacccgatgc
    13261 cgattgttgc ccaccagatg atccagtagg gattggaaac gctcatcaga attccgctga
    13321 ccaccggatg attcattttg ctttgatggg gttcccatga aagccgaagg gtgggcaagg
    13381 accgcagcat tcccgccgcc atccagatca gaatcaccgc gccggccagc gcgcttgccg
    13441 caaaaaccgc cggcatctga aaaaacgg
//
LOCUS       DAOSRM010000061        13165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4762_length_13165_cov_99.824790, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000061 DAOSRM010000000
VERSION     DAOSRM010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 13165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13165
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4762_length_13165_cov_99.824790"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /gene="dusB"
                     /locus_tag="PLA58_06990"
     CDS             complement(<1..880)
                     /gene="dusB"
                     /locus_tag="PLA58_06990"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOR62578.1"
                     /translation="MSNIISDLQGKALLAPLAGVTNLPFRLVARRLGCSLCFTEMISA
                     NGLIRESAQTLEYLKTSPEDRPLGVQLFGADPRVMARAAAMVAGQNPALIDINMGCPV
                     KKVIKTGSGAVLMKDPLLAGRIIANVVRAAKLPVTVKIRSGWSQGSINAVEIAKIAED
                     SGAAAIIVHGRTADQGFSGSADWDIIGRVKSAVRIPVVGNGDIRKPGDALRMLAQTGC
                     DAVMVGRGALGNPWIFQGIRQLLAGEKDCRHLSWSDRQEIVKQHWEEEVRYCGRHFAD
                     RSFRKHLLWYTKGLAGS"
     gene            complement(1249..1992)
                     /locus_tag="PLA58_06995"
     CDS             complement(1249..1992)
                     /locus_tag="PLA58_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOR62579.1"
                     /translation="MEKESQFCALILAAGKGTRMKSDMAKVLHVLEGNPLLHYSLKAA
                     REAGAEKIVVVVGHQADRVREAFPDPDLIFVDQMPQLGTGHAVLQARDALKDYGGLTV
                     ILCGDVPLLKPETIRSLIRQHRAEQSCVTVLTTEPPGPHAYGRIVKDGRGDILKIVEH
                     KDATEAERRILEINTGIYCVDTPFLFAALAQVKNDNRQKEYYLTDIVEIARREDRKVH
                     AFLTADDVEVMGINTLEELEKAGKYLKNR"
     gene            complement(2003..3298)
                     /locus_tag="PLA58_07000"
     CDS             complement(2003..3298)
                     /locus_tag="PLA58_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOR62580.1"
                     /translation="MSLYLMIAVIVICLILEALYSGGEVALFSSDINKIKYYASKGSS
                     AARQAVRLKEHPEWFISTALIGTNLAIIAASTLTTGLLIKTFGAKLGEQIAFLIMLPT
                     LFMIIIIRSIFQHYAEAMAIRVAHFIRLSSYIFYPLAYCVAAISRGAVNLSSDPKIRQ
                     RSYITKEGLKYILGEKTEGGDILTTEKEMVSRVFDFSELTAEKIMVPISALTALPQQM
                     KLDEAARVVAAKKYMRIPVYSDTVYNIVGILHYFDLLDALLKRQRAPEAENDATVAGC
                     MRKDVFYVPETKKAGPLLMDMQRKREHMAVVVDEYGGAVGIVTMEDIGEEIVGSMDEQ
                     YNKGEKLYKKAAPGRYLVNGRMKIEDLNQILPTKLPEGNYETLGGFLIAQAGRIPLRR
                     ERLEFGGTIFVIEDADQKSVREVLVIAPQPLEGQRTPIG"
     gene            complement(3295..4560)
                     /locus_tag="PLA58_07005"
     CDS             complement(3295..4560)
                     /locus_tag="PLA58_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOR62581.1"
                     /translation="MSSDIIILLILFLFSGFFSSAEAALLSLSDLHLHKMKSDNYPFL
                     NSVVKLLEHPRRLLITVVTSNEAVNISISILAASLFIALFGVNGQWISIVVTSVVLFL
                     AGEAIPKTFGVTYPMQISSAVAPLLLLISRLEFPIVAALEKTPDLILKRLDQRRADGT
                     DILTEDEFKHLIDAGSREGVVDESQKELIKTIFESGDRPVTDIMIPRVEMFCLPLDMP
                     VADIIREVVAGRCERVPVYRDDRDDIVGILFARDLLNSALASGPGASIEKSLVRPYFV
                     PESKTINGLLSDFQEKSIQIAIVVDEYGGVSGLVTLEDILEHLFEEAYGDDDTANCEC
                     DMPDAGTLVVPGRMSIEQVNELLQASLPLEEFDTIGGFVLHLFGKMPERGDLIHFERY
                     TFRVESVGRTRILKIRIEKEKPARTEEDE"
     gene            4745..6073
                     /gene="purD"
                     /locus_tag="PLA58_07010"
     CDS             4745..6073
                     /gene="purD"
                     /locus_tag="PLA58_07010"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOR62582.1"
                     /translation="MPNILLIGNGAREHAIAEAIARSGQAPSLLSWMKARNPGIASLS
                     RKIQIGSYTDLAAITGFARENKIDFAVIGPEDPLNNGVVDALAQIGVPAVGPNKSLAR
                     LETSKSFTRNLVNQYRIPGNPRYRVFPSLDGVKDFLNGLDAVVLKPDGLTGGKGVLVQ
                     GDHFATKDEALALCAQILKDSASVIVEEKFDGEEFSLQCLCDGKTVVGTPLVQDHKRR
                     FDGDCGPNTGGMGSYSLPDHSMPFLRPSDIEEGLEITRRVAAALWEETGSPYKGVMYG
                     GFIATKNGARLLEYNARFGDPEAMNILPLLKTDFVEVCKHIIDGTLDQLNIEFEKKAT
                     VCKYIVPKGYGLPADHSDTASSKARIEVGDTGKARLYYSSVDQKEDGLYLSSSRAVGI
                     VGIADTLEAARRIAEEGVKAVKGPIAYREDIGTLALIQKRIDHMKKIRMP"
     gene            6201..7400
                     /locus_tag="PLA58_07015"
     CDS             6201..7400
                     /locus_tag="PLA58_07015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR62583.1"
                     /translation="MRKLPAGIWALGFVSFFMDISSELIHSLLPIFMLTTLGASMIAI
                     GIIEGIAEAAASIAKVFSGIISDYIRKRKILAVLGYGLAAITKPLFPLATTIGWVFSA
                     RFIDRIGKGIRGAPRDALVADITPSHQRGAAYGLRQALDSAGAFMGPLAAVVLMIFFV
                     NDIRKVLWIAVIPAFIALGVLILGVHEPERQKTPNPTENRFFSFHGARRLPSRYWLIL
                     GLNAIFTLARFSEAFLILRAQSVGLAVGFVPAIMIVMNAVYALTAYPAGILTDRMRPR
                     IILAAGLAVLIAADTLLAFAETPFPVFIGAALWGLHMGLTQGLFAKLVADHAPVRLRG
                     TAFGIFNLVTGGALLTASVVAGLLWSAVGAQATFMAGAAFTALALLGVIVFKSRTPST
                     PHPGQAP"
     gene            complement(7415..8593)
                     /locus_tag="PLA58_07020"
     CDS             complement(7415..8593)
                     /locus_tag="PLA58_07020"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF005492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HOR62584.1"
                     /translation="MNEIQLKYGCNPNQKPARIFMADGGSLPVTVLNGKPGYINFLDA
                     LNSWQLVKEIKENTGKVAAASFKHVSPAGAALGFPLDDVLGRMYHIAPGMELSPLACA
                     YARARGADRMSSFGDWIALSDPCDVPTAKIIKNEVTDGIIAPGYEPDALEILKSKKGG
                     NYNVVSIDPRYVPAAIEHKQVYGITFEQGRNELKIDAGMLENIVTENKTLSDAQKRDL
                     VLALITLKYTQSNSVCFVQDGQVIGVGAGQQSRIHCTRLAGQKADLWQLRHMPKVLDL
                     PFRDDVAKPNRDNAIDVYLGDTPEDVIGEGVWAETFTRRPEPLSTQERKAWLSKVTGV
                     CLGSDAFFPFGDNIERARRSGVTAIVEPGGSIRDQQVIDTCNKYGIVMAFCGLRLFHH
                     "
     gene            complement(8597..9328)
                     /locus_tag="PLA58_07025"
     CDS             complement(8597..9328)
                     /locus_tag="PLA58_07025"
                     /inference="COORDINATES: protein motif:HMM:NF019443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOR62585.1"
                     /translation="MKTNLYEYLAGNEYPGRGLSIGKTPDGKKSVIAYFIMGRSTNSR
                     NRVFDPIDGGIRTKAADPSKMTDPHLIIYNPVLTLDGTTIVTNGDQTNTIYDFLAANR
                     HPGYNFEAALATRTFEDDKPNWTPRISGVLDMRSGGYKISILKSCDGNADSVQRFTFD
                     YPAPPAGEGHFISTYKCNGNPIPSFEGEPLRVAIDEEDPNEYAGKLWEALNEDNKVSL
                     FVRAVDLSNGSYEDVIMNKYQAVEG"
     gene            complement(9438..10931)
                     /locus_tag="PLA58_07030"
     CDS             complement(9438..10931)
                     /locus_tag="PLA58_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR62586.1"
                     /translation="MTSRSFFTSLFEPRAIAFIGASNNPAKWGFNILHHLIRGQYAGR
                     LYPINAQGGAWFGRKMFKSLAEVTEPLDLAVIVVPKEKVPDAIRECSRAGIAAAVVIT
                     AGFGETGPEGKILEQEVLRVARSCGVRIVGPNTMGIYSAYPSNLQAIMTSTQFKPGAV
                     AVVSQSGNLGTSITDRLVRREIGLSRLVSSGNEADLTVEDYLDYLETDEKTKVICLYV
                     EGVRQGHRFMETLRRISSVKPVILLKGGTGVVGAGAAMSHTGALAGSFAVFRAVCRQA
                     NIIMADTIDEMVDITGLFLSQPEIAGNRIGIVTQGGGLGVLSADLCEAVGLETPQLSD
                     HIVAMLDAFLPPFWSRRNPVDLVAPGRVSMITDSTAALLAHADLDAIVLLGMGYMTSR
                     ATRWLASPVLPREAMAGPAQKMIEGEMELVDLIVRQIREFNKPIIPVVDVVGFDEPGD
                     LNIVRRLDAQGVMAYSSPDQAIRALAKAQKYYSRRQAENQSIRKGGT"
     gene            complement(11050..11586)
                     /locus_tag="PLA58_07035"
     CDS             complement(11050..11586)
                     /locus_tag="PLA58_07035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR62587.1"
                     /translation="MKNTLTAVIETSKGAIRLKLFADQTPLTVGNFVNLARRGYYNQL
                     NFHRVISDFMIQGGCPNGDGRGGPGYTFPDEFVAGLRHSKPGILSMANAGPRTNGSQF
                     FITHVPTPWLDGKHTVFGEVESGEDQKVVNAIAQGDAIKSITIEGDATELMESIRKVL
                     DQWNKTLDVKFPKLQKAL"
     gene            11732..12247
                     /gene="purE"
                     /locus_tag="PLA58_07040"
     CDS             11732..12247
                     /gene="purE"
                     /locus_tag="PLA58_07040"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOR62588.1"
                     /translation="MKAKSAGKKIWVSVVMGSDSDLPVMTEAAKILDEFGIGYELVLT
                     SAHRTPERTTKFAKTAAGRGVKIILVGAGAAAHLAGVIASQTLLPVIGVPIDATSLRG
                     LDALLSTVQMPGGIPVATMAIGKAGAKNAALFAARALALEDKSLNAKLTAYVKKMARD
                     VEKKQQNLKVL"
     gene            12276..12911
                     /locus_tag="PLA58_07045"
     CDS             12276..12911
                     /locus_tag="PLA58_07045"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOR62589.1"
                     /translation="MPRILKINTGHSEEDVIGSAAAIISRGGVIAYPTETIYGLGADA
                     TNEQAIRKIFEIKGRNFSNPVSLIIGRREDVHPLVRAIPKTAQKLMDAFWPGPLTIVF
                     EAAGCVSPLLTAKTGKIGIRLSGHDGARQIALAAGKPLTATSANLSGLPECATADEVI
                     AQLGDRLDAVVDLGNTSGTAGSTIIDTTTEQPVILRAGVISREEILQKTGI"
BASE COUNT         2842 a         3892 c         3566 g         2865 t
ORIGIN      
        1 ccgaaccggc caaacccttg gtgtaccaga gcaaatgttt gcgaaaagac ctgtcggcaa
       61 aatgcctgcc gcaataacga acttcttcct cccagtgttg tttcacgatc tcctgccgat
      121 ccgaccagga gagatgacga caatcctttt ctcccgccaa taattgacga atcccctgaa
      181 atatccatgg atttcccagt gcgcctctcc cgaccatcac cgcatcgcaa ccggtttgcg
      241 ccagcatccg aagcgcgtcg cctggtttgc gaatatcgcc gttgccgacc accggaatgc
      301 ggacggcgct ttttacccgg ccgataatgt cccagtccgc ggagccggaa aagccctgat
      361 cggccgtgcg gccatgaaca atgatcgcgg cagccccgga atcctcagca attttggcta
      421 tttcaacggc attgatggag ccttggctcc acccggaacg gattttgacg gtcaccggca
      481 gctttgccgc ccggacaaca ttcgctatga tgcggcccgc aagcagggga tctttcatca
      541 acacggcgcc ggaaccggtt ttaatgacct ttttcaccgg gcagcccata ttgatatcga
      601 tcagagcggg attttgcccg gcaaccattg cggcggcccg ggccatgact cgggggtctg
      661 ccccgaacaa ctggacaccc agaggccggt cctccgggga tgttttcaaa tattcaagcg
      721 tttgcgccga ttcgcgaatc agaccgttgg cgctgatcat ctcggtaaaa cacagcgaac
      781 agccaagccg gcgggcaacc agccgaaacg gcagattcgt cacgccggcc agcggcgcga
      841 gcagcgcctt gccctgaaga tcagaaatta tattggacac aaatcaaacc gttctccatc
      901 aatcgctaat ttatggctca tgttccccgc caggaacggt cgcgaccgtt ccctacattt
      961 ccatcagaac cctttgcata atgcttctct gtcattgcga acgaagtgaa gcaatctcat
     1021 aagcatttga taatttgaag agatcgccac gtcgcttcgc tcctcgcgat gacaaaacag
     1081 taataatgca aagttctcca tcattcacaa cagccggtct gaagacccgc actacattga
     1141 atagcgccat ggttcgacaa gcaatgtcac catgagcctg tcgaatggtc accatgacat
     1201 tcaaaaacac tatccaccat tcaccatcca cccatagcct cttgcccatc acctgttttt
     1261 cagatacttc cccgcttttt ccagctcttc gagcgtgttg attcccatca cctcgacatc
     1321 atccgccgtc aggaaggcat gaaccttcct gtcttcgcgg cgggcaattt ccacgatgtc
     1381 cgtcaggtag tactcctttt gccggttgtc gtttttgacc tgggccagcg cggcaaacag
     1441 aaacggcgta tcgacgcagt aaatgcccgt attgatctca aggattcttc tttccgcctc
     1501 ggttgcgtcc ttgtgctcga caattttcag gatatcgccc cggccatcct tgacgatgcg
     1561 gccatacgcg tgcggcccgg gaggctcggt tgtcaaaacg gtgacacagg attgctcggc
     1621 ccgatgctga cggatcagcg accgaatggt ttccggcttc aaaagcggca cgtcgccgca
     1681 gagaataacc gtcagaccgc cataatcttt aagagcgtcc cgggcctgca aaacggcgtg
     1741 tccggtcccc agctgcggca tctgatccac aaaaatcaaa tccggatcag gaaacgcttc
     1801 tctcaccctg tccgcctgat gcccgacaac gacaacgatt ttttccgcgc ctgcctcgcg
     1861 cgccgctttc agggaatagt gcagaagcgg atttccctcc agaacatgaa ggactttggc
     1921 catatccgat ttcatgcggg tgcccttgcc cgccgccaga atcaacgcac agaactgcga
     1981 ttctttttcc aacacctgac tcctacccta tgggtgtccg ctgcccctcc aggggctgcg
     2041 gggcaatcac cagaacttcc cgaacggact tctgatccgc gtcttcaatc acaaaaatcg
     2101 tcccgccgaa ttccagcctt tcccggcgca ggggaattct ccccgcctga gcaatcagaa
     2161 aaccgccgag cgtttcataa ttaccctcgg gcagctttgt cggcaggatc tggttcaaat
     2221 cttcaatctt catccgtccg ttcaccagat accggccggg ggccgccttt ttgtagagct
     2281 tttccccctt gttgtactgt tcatccatgc tgccgacaat ttcttccccg atatcctcca
     2341 tcgtcacaat cccgaccgcg ccgccgtatt catcgacaac caccgccatg tgctcgcgct
     2401 tcctctgcat atccatcagc agcgggccgg ccttttttgt ctccggcaca taaaaaacat
     2461 ccttgcgcat gcagccggcc accgtcgcgt cgttttccgc ttccggcgcc cgctgccgct
     2521 tcagcagcgc gtccagaaga tcaaaataat gcaggatgcc gacgatattg tagaccgtgt
     2581 ccgaataaac gggaatgcgc atatattttt tcgccgcgac cacgcgggcc gcttcatcga
     2641 gcttcatctg ctgcggcagg gccgtcagcg cggaaatggg aaccatgatc ttttccgccg
     2701 tgagctcgga aaaatcgaag acgcggctga ccatttcctt ttccgtggtc agaatgtcgc
     2761 cgccttccgt cttttccccc aggatatact ttaagccttc cttggtaata taggagcgct
     2821 gtctgatttt cggatcggaa gaaagattca ctgcgccccg tgaaatcgcc gcgacgcagt
     2881 aagccagcgg ataaaatatg taggaggaaa gacggatgaa atgggccacc cggatggcca
     2941 tggcctccgc atagtgctga aagatgctgc ggatgataat gatcataaac agagtgggca
     3001 gcatgatcag gaaggcgatt tgctcgccta gtttggcgcc gaaagttttg atcaaaagcc
     3061 ccgtggtcag tgtggaagcg gcaatgatcg ccagattggt gccgataagc gcggtggaga
     3121 taaaccattc gggatgctcc ttcaacctga ccgcctgccg ggcggcggac gatcccttgc
     3181 ttgcgtaata tttgattttg ttgatgtcgg aagaaaacag cgcgacttcc ccgccggaat
     3241 agagggcttc cagaatcaga caaatcacaa tgacggcgat catcagatag aggctcattc
     3301 gtcttcctcc gtccgcgccg gtttttcctt ttcaatccgg attttcagga tgcgcgttct
     3361 gcccacgctt tccacccgga aggtatagcg ctcgaaatgg atcaggtctc cccgctccgg
     3421 catcttcccg aacaaatgca ggacaaagcc gccgatggtg tcgaattcct ccaacggcag
     3481 ggaagcctgc agcagttcgt tgacctgttc gatcgacatc cggccgggaa ccacaagcgt
     3541 gccggcgtcc ggcatgtcgc attcacagtt ggccgtatcg tcatctccgt aggcctcctc
     3601 aaacagatgt tcgagaatat cctccagcgt taccagaccg gacacgccgc cgtattcatc
     3661 cacaacgatg gcgatctgta tcgatttttc ctgaaaatcg gaaagcagcc cgttgatcgt
     3721 cttgctctcg gggacaaaat agggcctgac cagacttttc tcaatcgagg cgcccggtcc
     3781 ggaagcgaga gcgctgttga gcaggtcgcg ggcaaagaga atgccgacaa tgtcatcccg
     3841 gtcatcccgg tacacgggca cccgctcgca gcggccggcc accacttccc ggataatgtc
     3901 cgcgaccggc atgtccagcg ggaggcaaaa catttccacg cggggaatca tgatgtccgt
     3961 caccggcctg tcccccgatt caaaaatcgt tttgatgagc tctttctggg attcgtccac
     4021 cacgccttcc cggctgcccg catcgatcag atgcttgaac tcgtcttccg tcaggatgtc
     4081 ggttccgtcc gccctgcgct ggtccagccg cttcagaatc agatccggcg ttttttccag
     4141 cgcggcgacg atcggaaatt ccaggcggga aatcagaagc agcagcggcg ccacggccga
     4201 cgaaatctgc atgggatacg tcacgccgaa ggttttagga atggcctcgc cggccagaaa
     4261 aagaacgacg gacgtcacca cgatggaaat ccactgcccg tttacgccga acagcgcgat
     4321 gaacagggac gcggccagaa tggaaatgct gatgttgacg gcttcattgc tggtcaccac
     4381 ggtgatcagc agacgacggg gatgctccag cagcttcacc acggaattga gaaaaggata
     4441 attgtccgac ttcatcttgt gcagatgaag gtccgaaagc gacagaaggg ccgcctccgc
     4501 cgatgaaaag aaaccggaaa acaaaaaaag aatcagcaga ataataatat ccgaactcaa
     4561 aatggattaa ttcacctcgc cgaaatggtt catggtttct atgtatcaga ttacgcaaaa
     4621 aagatacagc cctttccggg caaagcgccg tccgcgggtc acggtggcct tcccgaatat
     4681 tccgttgaca tccacgggca accttcctat actccgccgc aatgaaacct ttgaggagat
     4741 ggatatgccg aacatcctgc tgattggaaa cggcgcgcgg gaacacgcaa tcgccgaagc
     4801 catcgcgcgg tccggacaag ccccttccct gctttcctgg atgaaggcca gaaatcccgg
     4861 aatcgcgtcc ctttcccgaa aaattcaaat cgggtcttat acggaccttg cggctatcac
     4921 cggtttcgcc cgggaaaata aaatcgactt cgccgtaatc ggcccggaag acccgctgaa
     4981 caacggcgtg gtcgatgcgc tggcgcaaat cggcgttccc gccgtcgggc cgaacaaaag
     5041 cctggcgcgg ctcgaaacat ccaaatcgtt cacgcggaac ctggtcaacc aataccgcat
     5101 ccccggcaat ccgcggtacc gggtttttcc gtcgctcgac ggcgtgaaag actttttgaa
     5161 tggcctcgac gccgtcgtcc tcaagccgga cgggctcacg ggcggcaagg gcgtccttgt
     5221 gcagggcgat cacttcgcca cgaaggatga agcgcttgcg ctgtgcgcgc aaatcctgaa
     5281 ggacagcgcc tcggtgatcg tggaggaaaa attcgacgga gaggaatttt cgctgcagtg
     5341 cctgtgcgac ggaaaaaccg tcgtcggcac gccgctggtg caggatcata aaaggcggtt
     5401 tgacggcgat tgcggcccca acacgggcgg catgggctct tattccctcc cggatcattc
     5461 aatgccgttt ctgcggccgt cggacattga agaaggactg gagatcacgc gccgggtggc
     5521 ggcggcgctt tgggaagaaa ccggcagccc gtacaagggt gtgatgtacg gcggtttcat
     5581 cgccacgaaa aacggcgcgc ggcttctgga gtacaacgcg cgcttcggcg atccggaagc
     5641 catgaacatc ctgccgcttt tgaaaacgga ttttgtggag gtctgcaaac acatcatcga
     5701 cgggacgctc gatcaactga acatcgaatt tgagaagaaa gccacggtct gcaaatacat
     5761 cgtcccgaaa ggttacggcc ttcccgccga tcattccgac acggcatcgt ccaaagccag
     5821 gatcgaagtg ggcgacacgg gcaaggcccg gctgtactac tcctccgtgg atcaaaaaga
     5881 agacggcctc tacctgagtt cgtcgcgcgc cgtcggcatt gtcggcattg ccgacacgct
     5941 ggaggccgcc cgccggatcg ccgaggaagg cgtcaaggcc gtgaaaggcc ccatcgccta
     6001 ccgggaagat atcggcacgc tggccctgat ccaaaaacgg atcgaccaca tgaagaagat
     6061 acggatgcca tagggacagg ggccatgcac caggtcatgc aaaccacttc gccaagtctt
     6121 tctcatccgg tcatttactt gcggtcactg aagttttttc tgttgtcgcc gatatttcag
     6181 tttggctgag gagaatgaag atgcgaaagc tgccggcagg catctgggcg ctgggatttg
     6241 tatccttttt catggatata tcttctgagc tgattcacag tctgctcccc atctttatgc
     6301 tgaccacttt aggcgcctcc atgatcgcca ttgggatcat cgaaggaatt gccgaggccg
     6361 cggcttcaat tgcaaaagtt ttttcgggca tcatcagcga ctacatcagg aaacgaaaaa
     6421 tccttgccgt gctgggctat ggacttgccg caatcacaaa accgctgttt cctttggcaa
     6481 caacgatcgg ctgggttttc agcgcacggt ttatcgaccg gatcggcaaa ggaattcgcg
     6541 gtgcgccacg cgacgcactg gtagcggata tcaccccttc ccatcagcgg ggggccgcct
     6601 acggcctccg gcaggcgctg gattccgcgg gagcgttcat ggggccgctc gcggcggttg
     6661 tcctcatgat cttttttgtg aatgacatcc gaaaagtcct ctggattgcc gtcatccccg
     6721 cctttatcgc tttgggggtt ctgattctcg gtgttcacga acccgaaagg caaaaaaccc
     6781 cgaacccaac ggaaaatcga tttttttcgt ttcacggcgc cagacgcctc ccctcccgct
     6841 actggctgat cttaggactc aatgcaattt ttacgcttgc ccgcttcagc gaagcgtttc
     6901 tgatcttgcg cgcccagtcg gtcgggctgg ctgtgggatt tgtgcccgcc atcatgatcg
     6961 tcatgaacgc cgtttatgcg ctcaccgcct acccggcagg aatcctcacc gaccggatgc
     7021 ggccacggat catactggct gcgggacttg ccgtcctgat cgccgcggac accctcctgg
     7081 cctttgccga aacacctttt ccggtattca tcggcgcggc actctgggga ttgcacatgg
     7141 gactgacaca gggattattc gccaagctgg tggccgatca cgctcctgtc cggctgagag
     7201 gcacggcctt tggcatcttc aacctggtaa cgggcggcgc tcttttaacg gcaagtgtcg
     7261 tcgccggctt attgtggagc gccgtcggcg cacaggcaac atttatggcc ggcgcagcct
     7321 ttacggccct tgctttgctc ggtgtgatcg tctttaaatc ccggacgcct tcgacgcctc
     7381 atcccggtca ggcaccctaa aataactctt ttcactagtg atgaaacagc ctgagcccgc
     7441 aaaacgccat gacgatgccg tacttgttgc aggtgtcgat cacctgctga tcgcggatgg
     7501 agccgcccgg ttcgacaatg gcggtgacgc cgctgcggcg cgcgcgctca atattgtcgc
     7561 cgaaggggaa aaacgcatcg ctccccagac agacgccggt aaccttgctg agccaggcct
     7621 tccgctcctg cgttgaaagc ggttcggggc ggcgcgtgaa ggtttcggcc cacacgccct
     7681 cgccgatcac gtcttcgggg gtgtccccca gataaacatc gatggcgttg tcgcggttgg
     7741 gcttggcgac gtcgtcgcgg aagggcagat ccagcacttt gggcatgtgc cgaagctgcc
     7801 agaggtcggc cttctgtccg gccagccgtg tgcagtggat gcggctctgc tggcccgcgc
     7861 cgacaccgat cacctggccg tcctgcacga agcagacgct gttggactgc gtgtatttga
     7921 gcgtgatgag cgccagcacg aggtcgcgct tctgcgcgtc ggaaagcgtc ttgttttcgg
     7981 tcacgatgtt ttccagcatg cccgcgtcga tcttcagctc gttgcggccc tgctcgaacg
     8041 taatgccgta aacctgcttg tgctcgatcg cggcgggaac gtagcgcgga tcgatggaca
     8101 cgacattgta gttgccgccc ttcttggatt tcaggatttc cagcgcgtcc ggctcgtagc
     8161 ccggcgcgat gatgccgtcg gtgacttcat ttttgatgat ctttgccgtg ggaacgtcgc
     8221 acgggtcgga cagcgcgatc caatcgccga agctgctcat gcggtcggcg ccccgggccc
     8281 gggcgtaagc gcaggcgagc ggcgaaagct ccataccggg cgcgatgtgg tacatgcggc
     8341 cgagcacgtc gtccagggga aaacccaaag cggcgccggc aggcgatacg tgcttgaagg
     8401 aagccgcggc caccttgccg gtgttttcct tgatctcctt cacgagctgc caactgttga
     8461 gtgcgtcgag aaaattgata tagcccggtt ttccgttgag caccgtcacc ggcaggcttc
     8521 cgccgtccgc catgaagatt cgggcgggct tctgattggg gttgcatccg tatttcaatt
     8581 gtatttcatt catgggttat ccctccacgg cctgatattt gttcatgatg acgtcttcat
     8641 aggaaccgtt cgaaagatcg acggcgcgga caaacaggct caccttgttg tcttcgttca
     8701 aggcctccca gagtttgccc gcgtactcgt tgggatcttc ctcgtcgatg gccacgcgca
     8761 gcggctcgcc ttcgaaactg ggaatcggat tgccgttgca tttgtaggtg ctgatgaagt
     8821 gcccctcacc ggccggcggc gccggataat caaacgtaaa acgctggacg ctgtcggcgt
     8881 ttccatcgca gcttttcaaa atgctgatct tgtagccgcc cgagcgcatg tccagcacgc
     8941 cggaaatgcg gggcgtccag ttgggcttgt cgtcttcaaa tgtgcgcgtg gccagcgcgg
     9001 cttcaaagtt atagccggga tggcggttgg ctgccaggaa atcatagatg gtgttggtct
     9061 ggtcgccgtt cgtcacaatt gtcgtgccgt ccagcgtcag aacgggattg taaatgatca
     9121 gatgcgggtc cgtcattttg gaaggatcgg ccgccttcgt gcgaatgccc ccgtcgatcg
     9181 ggtcgaaaac gcggttgcgg ctgtttgtgc tccggcccat gatgaaataa gcgatcactg
     9241 attttttgcc gtccggggtt ttcccgatgg acaggccgcg gcccggatac tcgtttccgg
     9301 ccagatactc gtataagttc gttttcatga agttttctcc ctgttgcaaa tgatggattg
     9361 ccgttttgtg gcgggagtat atgaagagac gtttcgtctg tcaactatga tgtcggggga
     9421 tacaacactt cattcaatta agttccccct tttcgtatcg actgattttc ggcctgcctc
     9481 cggctgtaat acttctgggc tttggccagc gcccggatcg cctgatccgg cgaggagtac
     9541 gccatgaccc cctgcgcatc caggcgcctg acaatgttca gatcgcccgg ttcatcgaag
     9601 ccgacaacat cgaccaccgg gataatcggc ttgttgaatt ccctgatctg ccgcacgatc
     9661 aggtccacca gctccatttc gccttcgatc attttttgag ccggtcctgc catggcttcg
     9721 cgcggaagca cgggggacgc aagccagcgc gtggcgcggc tggtcatgta ccccatcccc
     9781 agaagcacaa tggcgtccag gtccgcgtgc gccagaagcg ccgcggtgga atccgtgatc
     9841 atggaaaccc tgcccggcgc gaccagatcc accggattgc gcctgctcca gaaaggcgga
     9901 agaaacgcat cgagcatggc aacaatgtga tcggaaagtt gaggcgtttc caaaccgact
     9961 gcctcacaga gatcggcgga aagaaccccc aaccctccgc cttgcgtgac aatgccgatc
    10021 cggttccccg caatttccgg ctgcgagaga aaaagaccgg tgatgtccac catctcgtca
    10081 atcgtatccg ccataataat attggcctgc cggcagacag cgcgaaagac cgcgaagctt
    10141 ccggccagcg cgccggtatg cgacatggcc gcgcccgcgc cgacaactcc ggtgcctccc
    10201 ttgagcagaa tgaccggttt gacgctggat atacgcctga gcgtctccat gaaccgatgc
    10261 ccctggcgaa cgccttccac gtaaaggcag atgactttcg ttttttcatc ggtttccaag
    10321 tagtccaggt agtcttccac cgtcagatcc gcctcgttgc ccgaacttac cagacggctc
    10381 aaaccgatct cccgccggac aagccggtcg gtgatcgagg tgcccaggtt gccgctctga
    10441 gaaaccacgg caacggcccc cggcttgaat tgcgtggagg tcatgatcgc ctgtaagttc
    10501 gagggatagg cgctgtagat ccccatggta ttgggcccca cgatgcggac accgcaactt
    10561 cgcgcgacgc gaaggacttc ctgctccaga attttcccct ccggtcccgt ctcgccgaac
    10621 cctgctgtaa tcaccaccgc ggcggcaatg cccgcccggc tgcattcgcg aatcgcatcc
    10681 ggaacttttt ccttgggaac aacaatgacg gccaggtcaa gaggctctgt cacctcggcc
    10741 aggcttttaa acattttcct tccgaaccac gcgccgccct gggcattgat gggatagaga
    10801 cggcctgcgt actggcccct tatgaggtga tggagaatgt tgaaacccca tttggcggga
    10861 ttgttggacg cgccgataaa cgcgatggcc cgcggctcaa aaagggacgt aaaaaatgat
    10921 cgggatgtca cgacagctcc ttccttgatg gatgccgggt cagtatgtgg atggacagcg
    10981 ccacgggagg cacaaccgaa aataacaaag gatgccgcag ggttggctga aaaaacagtg
    11041 tccctcgatc tacagagcct tttgcaattt gggaaacttc acatccagcg tcttgttcca
    11101 ctggtccagg accttccgga tgctttccat cagttccgtt gcatcgcctt cgatcgtgat
    11161 ggacttaatg gcatcaccct gcgcgatggc attgacgact ttctgatcct cgccgctttc
    11221 cacttcgccg aacaccgtgt gcttgccgtc cagccagggc gtcggcacat gcgtgataaa
    11281 gaactggctg ccgttggtcc ggggaccggc gttggccatg gacaaaatgc cgggcttgct
    11341 gtgtcggagc cccgccacaa actcgtcggg aaaggtgtag ccgggaccgc cgcggccgtc
    11401 tccgttggga cagccgccct gaatcataaa atcggaaatg acgcgatgga agttcagctg
    11461 gttgtaatat ccgcgccgtg ccaggttgac aaaattgccg actgtcagcg gggtttgatc
    11521 ggcaaacaac ttcagccgga tggcgccttt gctggtttca ataactgccg ttaaagtgtt
    11581 tttcatgtct gtctcctttg atcagcgatg ggaaactcta gagagcatcc ccggatatgt
    11641 caagataaaa accatcttgc aaaaggaacg gaagtgcgtt aaggacgctt aaaattactc
    11701 acttatttca tattgttaca gaggagtccg catgaaagcc aaaagcgccg gaaagaagat
    11761 atgggtcagt gtggtcatgg gcagtgattc ggacctgccg gtcatgaccg aagcggcaaa
    11821 aattctggat gaattcggca tcggctatga gctggtgtta acgtcggcac accgcacgcc
    11881 ggaacggaca accaaattcg ccaaaacggc cgccgggcgg ggcgtgaaaa tcattcttgt
    11941 cggcgccggc gccgcggcgc acctggccgg agttattgct tcacaaaccc tgctccctgt
    12001 catcggcgtt cccattgacg ccacatccct caggggcctg gatgcactgc tctccaccgt
    12061 gcaaatgccc ggcggcattc ctgtggctac catggccatc ggcaaggccg gcgcaaagaa
    12121 cgcggccctt tttgcggcgc gcgctctggc attggaagac aaatccctga acgcaaaact
    12181 taccgcatac gtaaaaaaga tggccaggga tgtggaaaaa aagcagcaga acttgaaggt
    12241 tctgtaaaaa ccccgtcaaa tccgggaaac ccctgatgcc ccgaatcctg aaaatcaaca
    12301 ccggtcattc ggaagaagat gtcatcggca gcgccgccgc aatcatttcc cgcggaggcg
    12361 ttattgccta tcccaccgaa accatctacg gcctcggcgc ggatgcaacc aacgaacagg
    12421 ccatccgcaa aatcttcgaa atcaaagggc ggaacttttc caatcccgtc tccctgatca
    12481 tcggccggcg cgaagacgtc caccctttgg tgcgcgccat tccaaagacc gctcaaaaac
    12541 tgatggacgc tttctggccg ggaccgctga cgattgtttt tgaggcggca ggctgtgtct
    12601 ctcctcttct gacggcaaag accgggaaga tcggcatccg tctgtccggc catgacggcg
    12661 ccagacaaat cgctctggcg gcggggaaac cgctgacggc aaccagcgcg aatttatccg
    12721 gcttgccgga atgtgcaacg gcagatgagg tgatcgcgca actcggggat cggcttgacg
    12781 ccgtggttga tctgggaaat acaagcggta ctgcaggctc caccatcatt gacaccacaa
    12841 cggaacaacc ggttattttg cgggcaggtg taatttcccg tgaagaaata ttgcagaaaa
    12901 ccgggatctg aaaactgcag ggaacggtct tgccgcccgc aaggatgaaa acaacagtgc
    12961 aaaggggcga cgagtggaaa gtgatgagta gaaaaaggga aacatccttc tctttccact
    13021 caccactcgt tgctcattgc ttttcactta ttgtttttgc ccattgccca tgacctgttc
    13081 tcttcagcct tcagccttca gtcttcagcc ttcagccttc ggtcttcagc cttcagcctg
    13141 ttttcttccc atacccgata ttcct
//
LOCUS       DAOSRM010000062        12731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_4954_length_12731_cov_114.105633, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000062 DAOSRM010000000
VERSION     DAOSRM010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12731
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4954_length_12731_cov_114.105633"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /gene="mnmG"
                     /locus_tag="PLA58_07050"
     CDS             <1..1788
                     /gene="mnmG"
                     /locus_tag="PLA58_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOR62590.1"
                     /translation="LLFNINLDSIALMSCNPAIGGLAKSQLVKEVDALGGEMGRITDK
                     TAVHFRLLNASKGPAVQSTRMQCDKQLYRLTMKATVENQANLHIRQAMVEALVVEDGK
                     VLGVLDASGHFYGAKKVVITTGTFLNGLVHIGPSQTPSGRAGELASVGLARQLQSLGF
                     DMGRMKTGTPPRLKASTIDFSVLERQDSDPDPAPFSFTTAALRTERLPSYFAATCEET
                     HRIIRDNIRQSPLYSGAIQGVSARYCPSLEDKVMRFGQRQSHPVVLEHEGLDTQEVYA
                     KGLGNSLPLELQYAIVHSVRGLEQAEIMRPAYAIEYDVVQPTQLRMTLETKRISGLYL
                     AGQINGTSGYEEAAAQGIWAGINATLALAGAPPFVLDRSEAYMAVMIDDLVTRGVDEP
                     YRMFTSRAEYRLILREDNAVLRLMGRGCELGLVDRARYLDLQNQKKRIEEGIRKLQSS
                     SVKPTPPINEKLAALSSPPITQATNLYSLLKRHEIAYDNLNGFPGWEPQNDRFVKKQI
                     EIEIKYEGYIRRQFEATKKLKEQEKIKIPPDFDYASVPGLSNELQAKLTRITPATIGQ
                     MGRIPGMTEGAVSAVLIMIKKKELLQSTR"
     gene            1978..2574
                     /locus_tag="PLA58_07055"
     CDS             1978..2574
                     /locus_tag="PLA58_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62591.1"
                     /translation="MKKIAASILATLFIVVMVTGCADEKGPAQLAMKAAEQAVTATKA
                     EAAKLVPEQMAALEAALASAKDKLAKGEYKAALTEAQGLVGKAKDVLAAAKAKKDELS
                     KQWTELTEGMPQMVEAIQGKVDSLSKLKKLPKNITTEKLAEAKSGLEAVKADLAKAQE
                     SFKSGNIAEAVAAAAAVKEKAVKAMESLGISAPEPAKS"
     gene            complement(2660..3322)
                     /locus_tag="PLA58_07060"
     CDS             complement(2660..3322)
                     /locus_tag="PLA58_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62592.1"
                     /translation="MKKLPFLIVVLLICACGGAGHIPAWKEKAYSQLDEYKTSFLTGR
                     EASTEPHFEKARKEIAAGNDLGLLTVAYLTKYALHTASLEPFDASEFAKLYRLEPHPA
                     DMAYCHFLKGNFNAVDTKELPARYAGVLKAASGRDAATAGREIAAIDDPLSRLIASGV
                     WTKYLPADEAILKIGIDTASANGWRRPLWAYLERLQSYYLENGDSAKANRVSERLKLL
                     KK"
     gene            complement(3319..4272)
                     /locus_tag="PLA58_07065"
     CDS             complement(3319..4272)
                     /locus_tag="PLA58_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62593.1"
                     /translation="MTVSRELKVGLFIVCTAVLLAAALLYLAVGKGVFETMHTFTLSS
                     RSGDGFTEGMPVDFSGFNIGKVQALELNDKGIVLIKIKIPDRHVKWVRSDSTFILYRP
                     LIGVARIVVTTSDLSSPPLDPNQVPEVAIVNDINDAIARIEPVLDQVTKIADHVEQLT
                     RTLSDPRGNLNQLLGNAEKITANLASKKSLVEMAVSDEESVRALNDSLKQLKEITINV
                     NRILAKVDKMADKTDHEIYGKDGALPQVNVILKDVIGKLQKLDKTIDNINKISMDASE
                     GMKDFHLLRSDIDDVVTSLDSVVKKLDAIVGSKKNPEFKVP"
     gene            complement(4269..4601)
                     /locus_tag="PLA58_07070"
     CDS             complement(4269..4601)
                     /locus_tag="PLA58_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62594.1"
                     /translation="MNIALIKQYHENMPRRQARQLALSCLERFGLGPIADRRNPALNT
                     EERFCVMLLRAAMVKDAMILIDQPFQIVPHLKDGRFIMNALKIIDDLYLSCQIYDYRW
                     MKEKYGEL"
     gene            complement(4721..5497)
                     /locus_tag="PLA58_07075"
     CDS             complement(4721..5497)
                     /locus_tag="PLA58_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR62595.1"
                     /translation="MLEKLGNQVVRMISGFAGTIAFSGKVFLRIFQQKTYSSAMREVL
                     LNQLYFTSVQILPLFLIVSILFGSLFIGVVFTLLKELGLTQFIGHILMGLIVTELSPF
                     LTVLLITLRSSAAINTEMAVMKVNQEIKTLEIFRIDIIDYLVMPRIINGIVSIVLLSS
                     LFSIVLLVSGNLFSRMMFGMSSDVYSNLLLNSTDFSDIVIALFKCAVYGFFITLIPIR
                     FGLRASRELTSIPVVVSQGMVNVFAAILMIEVLSLITKLL"
     gene            5674..7140
                     /locus_tag="PLA58_07080"
     CDS             5674..7140
                     /locus_tag="PLA58_07080"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOR62596.1"
                     /translation="MKNSNRKTFFMFLMAFLVAFFIVSLVGVLRSSFTSSGAPEIPTA
                     QAVPSLSNTPGTAPNSFSDLVDRVKPAVVNISTSKTYKGRGGFGTPFGESPFGDDFFD
                     RFFGDMPRREFKQRSLGSGFIISNDGYIFTNNHVVEQADKIRVKISDGKEYDAKVIGT
                     DVNTDIALIKIKPTNSLPVADIGDSEKVRVGEWVVAIGNPFGLDATVTAGIVSAKGRV
                     IGAGPYDNFIQTDASINPGNSGGPLFNMEGKVIGINTAIVAHGQGIGFAIPINMAKNI
                     LADLKTKGKVTRGWLGISVQDITDDVAQNLNHKNKNGALVSDVFKGDPADAAGIKIGD
                     IIIEINGKPVKDTHELLLMIAAMKVGQKMSVKAIRDGKEMMFQVVVAERKDNVTMAME
                     RSGLGHFGIAAQDITPEMAKQLGIARDGVMVTDVQAGSPADEVGIQPRDIIVQVNRVK
                     ISSMKDYTREITKAAQKKSVTLFIKRGRSGYSVTIRID"
     gene            7217..8299
                     /locus_tag="PLA58_07085"
     CDS             7217..8299
                     /locus_tag="PLA58_07085"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOR62597.1"
                     /translation="MDRKLHDYGGVIHLHSSFSFDGHAPMEEILAAAEKNEVDFIMLT
                     DHDHLKARQEGWEGWQKKTLVLVGEEIAPRFNHFLAFNIQEPVICSGDPEGINPQKYI
                     DEVNRQGGFGFIAHPDHEGTKLFHVKHYSWNNWNVGDFAGIGVWDFMTDWQKSLHGYG
                     SALLSFLFPALVLKGPRRITLERWDALNQIRKTVGIGELDNHATVKKLLGIRFVAFPF
                     HRAFAFIRTHVLTKTGFTGNNQQDIAMLYESLLHGRCYFALEYFRRAKGFQFAVEQDQ
                     KVFHMGDSLPLSLSGNAQLSVSCPAKARIRVIRDGREECRAIGNRLSIKIDKTGVYRV
                     EAHLKTAGKTRPWIFSNPIFINSPNH"
     gene            complement(8277..10472)
                     /locus_tag="PLA58_07090"
     CDS             complement(8277..10472)
                     /locus_tag="PLA58_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOR62598.1"
                     /translation="MNPSEIITKAIEEGRSALTEAEAKVVLKHYHVPVVEEKLINNIN
                     DIIVSAQEMGFPVVLKGLGSRLTHKTERGVVKLNIKNPDDLQAAAVYIKDAAGDDLEG
                     FLLQPMLEGKREFVAGLFFDEQFGPVIMFGLGGIFTEAIGDVVFRLAPIDEEEANRMI
                     SEVSAKKLLSDFRGEKAPDRNALVRVLTGLSEMAVSIPEIKEIDINPLLVSPDGRVTA
                     VDALIVLGERSLQKTTHVPVEPIAIAKLFYPGSIAFIGASAELSKWGQLMYTNVIAGR
                     YSGKVYLVNPKGGEIAGRKAFKTVAEIPGPVDLAVITIPAGKVLPIIDELQQKGIKNA
                     LLISSGFGETGAEGKALEEELVKKAREAGVLILGPNTMGICNPHCSLYCTGTHVRPKP
                     GDTVLVAQSGNLGTQLLAFAEKEGIGIRAFSGSGNEAMITIEDYLDGFEVDGMTKTVV
                     LYIESIKNGRRFFESARRVGRNKPIIALKGGRTQAGAHAAASHTGAMASNIRVFNAAC
                     KQAGIIQANQSMELLDLSAAFSSLPLPHGNRVAILTLGGGWGVIASDLCAENGLIVQP
                     LSTEIIQKINGLLPAYWSHANPVDLVAEMNTDTHVAIVEELLQWKECDAIIYMGIIGR
                     KVTIQSVLESTVAVDKSYDPKMVAENLELLRVYERGLVEKTVRVMGKYHKPVIGVYLL
                     TDETTRTVIEIEGQKYKGIVFPSPERAVKSLSMLFRYSRWQKANGSESL"
     gene            complement(10656..11663)
                     /locus_tag="PLA58_07095"
     CDS             complement(10656..11663)
                     /locus_tag="PLA58_07095"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HOR62599.1"
                     /translation="MYENQIIPGYRHQPGFHCASSAIRNVLEFHGIKISEPTVIGLGR
                     GPGFFYLVLNQTPSRLVHMRCLALEENFFRAVGLKFRWRETENAAEATRHAKEAVDCG
                     LPVLVQTDIAYLPYFNTDQHFPGHAVVICGYDDKKEEFYVSDTLHPETLAVTITDMER
                     ARSAAFPPFLLRNKAFVLEDISGFRIDETVVHSAIRLWAEENLNGIPGLPAAYGVKSL
                     EALIRDLANWKEAEDWRWSARFTYQVIERRGTGGAGFRTLYRQFLEEQEAQYPALQRL
                     RLSREMAVIEGIYHEVAAVFRRISEEDTPNFSPATPILENLLEREKDFYQKILRLVLQ
                     N"
     gene            12176..12682
                     /locus_tag="PLA58_07100"
     CDS             12176..12682
                     /locus_tag="PLA58_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOR62600.1"
                     /translation="MTNPSVNQVNVDGRLIGIVDLEEALQKAISVCSGKSDLEISDFL
                     VREVSGRNYIPSSILPAYARALLREYKAALNLPVDPEPATGLTVLVLGAGCARCDQLQ
                     SDIRDVLSQMQIAADVRHVTDIREMSRFGVLGAPALVINNRVAAVGEVPPKSLIRQWI
                     TDALKNGD"
BASE COUNT         3131 a         3392 c         3240 g         2968 t
ORIGIN      
        1 cttttgttca acatcaatct cgattccatc gcgctcatgt cgtgcaatcc ggccatcggc
       61 gggctcgcca aaagccagct cgtcaaggaa gtggacgcgt tgggcggaga gatgggccgg
      121 atcacggata aaaccgccgt gcatttccgc ctgctcaacg cctccaaggg cccggccgtt
      181 caatccaccc gcatgcagtg cgacaagcag ctttaccgtc tgaccatgaa ggcgacggtg
      241 gaaaatcagg ccaacctgca catccgccag gccatggtgg aggccctggt tgttgaggac
      301 ggcaaagttc tcggcgtact ggacgccagc ggccattttt acggcgccaa aaaagtcgtc
      361 atcaccacgg gcactttttt aaacggcctg gtccacattg gcccgtcgca gacgccatcc
      421 ggcagggcgg gagaactggc atcggtgggg cttgcccgtc agctccaatc gctcggattt
      481 gacatggggc ggatgaaaac cggcacaccg ccgcgcctta aagcctcgac cattgatttt
      541 tccgttctgg agcgccagga cagcgatccc gatcccgcgc ctttttcttt tacgacggcc
      601 gctctgcgga cggagcgtct gccttcttat tttgccgcaa cgtgtgagga aacccatcgc
      661 atcatccgcg acaatattcg tcagtcccct ctttactccg gcgccattca aggcgtcagc
      721 gcgcgctact gcccgtcgct cgaagacaag gtgatgcgtt tcggccagcg gcagagccac
      781 ccggttgtgc tggaacacga aggactcgac acgcaggagg tctatgccaa ggggctgggc
      841 aattccctgc cgctggaatt gcagtatgcg attgtccaca gcgtgcgcgg tctggagcag
      901 gccgaaatca tgcggcccgc ctacgccatc gaatacgacg ttgttcagcc gacgcagctt
      961 cggatgacgc tggagaccaa acgcatctcc ggcctgtacc tggcgggcca aatcaacggg
     1021 acatccggct atgaggaggc tgcggcgcag ggcatctggg cggggattaa cgcaacgctt
     1081 gcacttgcgg gggcgccgcc tttcgtcctg gaccgctccg aggcttacat ggccgtgatg
     1141 atcgacgatc tggtcacgcg cggcgtggat gaaccctacc gcatgttcac ctcgcgggct
     1201 gagtaccggc tgattctgcg ggaggacaac gccgtcctgc gcctgatggg ccggggctgc
     1261 gagctgggac tggttgaccg cgctcgttat ctggatcttc agaatcaaaa gaaacggatt
     1321 gaagagggca tccggaagct ccaatcgtcg tcggtcaaac cgacgccccc catcaatgaa
     1381 aagctggcgg ctctgtcctc cccgccgatt acacaggcca ccaaccttta cagtcttttg
     1441 aaaaggcatg aaattgccta tgacaattta aacggtttcc ccggctggga gccccagaat
     1501 gaccgctttg tcaaaaagca gatcgagatc gaaatcaagt atgaaggcta tatccggcgt
     1561 cagttcgaag cgacaaaaaa actgaaggag caggaaaaaa taaaaatccc gccggatttc
     1621 gactatgcgt ccgttcccgg tctttccaac gaactgcagg ccaagctcac ccggatcacg
     1681 cccgcgacca tcggccagat ggggcgcatt cccggcatga ctgaaggcgc cgtctccgcc
     1741 gtcctgatca tgatcaaaaa gaaggaattg ctgcaatcga ctcgttaacc ggtttgaaaa
     1801 tgagttgaag cgaaaacggc ccggtgatgc ctcgcaaaac atgatggcgc catctgacgg
     1861 ctgaagcagg cgggaaatgt tcttgacagg aaaaaacacg gtttctataa tccgcgtaat
     1921 agcgttggaa cgaatcattg atcatcagca aacaaccaac attcaggagg aggggatatg
     1981 aaaaaaatcg ctgcaagtat tttagcaaca ttgttcattg tggtcatggt gacgggctgc
     2041 gccgatgaaa agggaccggc ccagctggcc atgaaagcgg cggagcaggc ggttaccgcc
     2101 acaaaggccg aggcggcaaa acttgttccg gaacagatgg ccgcgctgga agccgctctg
     2161 gcatccgcga aggacaaact cgccaagggt gagtacaaag ccgccctgac cgaagcgcag
     2221 ggtcttgtcg gcaaggccaa ggatgttctg gcggcggcaa aagccaaaaa agacgagctg
     2281 tccaagcaat ggacggagct gacggaggga atgcctcaga tggttgaagc catccagggc
     2341 aaggtggact ccctgtccaa actcaaaaag cttccgaaaa acatcaccac cgaaaagctg
     2401 gcggaagcaa agtccggact ggaggctgtc aaagcagatc tggcaaaggc gcaggaaagt
     2461 tttaaatccg gaaacatcgc ggaagccgtt gccgcggctg ccgccgtgaa agagaaagct
     2521 gtaaaggcca tggaaagcct gggcatttcc gctccggaac cggcaaagtc ataaaaaaat
     2581 caaaaggggc acattcccaa ctggtgtgaa acggggagtg tgcctctttt ttttcaagaa
     2641 tgatggattt gaggcgcggt cactttttta aaagcttcag cctttcggaa acacggtttg
     2701 cttttgccga atctccattt tccagataat agctctgaag cctttccaga taagcccaga
     2761 gcggccttcg ccagccgttg gccgacgcgg tgtcgatgcc gattttcaag atggcttcat
     2821 cggcgggcag gtattttgtc cagacgccgc tggcaatcaa ccgggacaag ggatcgtcaa
     2881 tggctgcaat ctcacggccg gctgtggcgg catccctgcc ggaagcggct ttcagaacac
     2941 cggcatatct cgccggaagc tctttcgtgt ccacggcgtt gaagttgcct tttaaaaaat
     3001 ggcagtaggc catatcggcg ggatgcggtt cgagccggta gagctttgca aactcgctcg
     3061 cgtcaaaggg ttccagcgat gccgtatgga gcgcgtattt cgtcagatag gcgaccgtta
     3121 aaaggcccag atcgttgccg gcggcgattt cttttctggc tttttcaaaa tgaggttcgg
     3181 tggacgcctc ccggccggtc aggaagctgg tcttgtactc atccaactgg ctgtatgctt
     3241 tttccttcca ggcaggaata tgcccggcgc ccccgcaggc gcaaatcaat aacacgacga
     3301 ttaaaaacgg aagctttttc atggaacctt aaactccgga tttttctttg accctacaat
     3361 agcgtccaat ttttttacaa cactgtccag cgaggtcact acatcatcga tgtccgagcg
     3421 aagcaggtga aaatctttca tgccttcaga tgcatccatg cttattttat taatattatc
     3481 aatagtttta tccagctttt gcagttttcc gatgacgtct ttgagaatga cattgacctg
     3541 gggaagcgcg ccatcctttc catagatttc atgatcggtc ttgtccgcca tcttatccac
     3601 tttggcgagg atgcggttga cattgattgt aatttccttc agttgtttga gggaatcatt
     3661 cagcgctctg acgctttcct catcggaaac ggccatttcc acaagggatt ttttcgaggc
     3721 caggttcgcg gttatttttt ccgcattgcc cagcagctga tttaagttcc cgcggggatc
     3781 ggaaagcgtg cgcgttaatt gctcaacatg atcggcaatc tttgtcactt ggtccagaac
     3841 aggttctatt ctcgctatgg cgtcattgat gtcgttgacg atggccactt ccggaacctg
     3901 gtttggatcc agcggcggac tgctcagatc ggaagtggtc acgacaatcc gcgccacgcc
     3961 gatcagcggc cggtacaaaa taaaggtgct gtccgaccgg acccatttca catggcgatc
     4021 ggggattttg attttaatta aaacgatgcc tttgtcgttg agctccagcg cctgcacctt
     4081 gccgatgttg aatcccgaaa aatcaaccgg catgccttcg gtaaagccgt cgccggagcg
     4141 cgacgacagc gtgaaggtgt gcatggtctc gaaaacgccc tttcccacgg caaggtacaa
     4201 aagcgccgcg gcgagcaaaa cggccgtgca gacgatgaaa agtccgactt tcaattcgcg
     4261 gcttactgtc atagttctcc gtacttttct ttcatccatc ggtagtcgta gatttggcag
     4321 cttaaataca aatcatcaat gattttcaga gcattcatga taaagcgacc atccttcaag
     4381 tgggggacaa tttgaaaagg ctgatcgatc agaatcatgg cgtccttgac catcgcagcc
     4441 cgaagcagca tgacgcaaaa acgctcttca gtgttcagcg ccgggttgcg cctgtcggca
     4501 atcggcccga gaccaaagcg ctccaggcac gagagcgcaa gctgccgggc ctgccgcctg
     4561 ggcatatttt cgtgatactg cttgatcagg gcgatgttca tgtacacatc ctgattggaa
     4621 atcagcggca cattctcgga aacaggcgca gcctgcaggg gaaaagcccg cagaaacttc
     4681 tcctgcgact gccggagcgt ctgttcgttg tcaaaataat ttacagtaat tttgtgatta
     4741 atgacagcac ctcgatcatc aggatagccg caaaaacatt taccatgccc tgggatacca
     4801 ccacgggaat gctggtcagt tcacgtgacg cccgcagacc gaaacggatg ggaatcagcg
     4861 taatgaaaaa tccgtaaacg gcgcacttga acagggcaat gactatatca gaaaaatccg
     4921 tggaatttaa cagaagattt gaataaacat ccgagctcat cccgaacatc atccgggaaa
     4981 acaggttgcc gctgaccagc aggacaatgg aaaaaagact gcttagcaaa acgatggaaa
     5041 cgatgccgtt gatgatgcgc ggcatcacca gataatcgat gatgtcaatg cggaaaattt
     5101 ccagcgtttt gatttcctgg ttgaccttca tcaccgccat ttccgtattg atcgcggcgc
     5161 tcgaccgcag ggtgatcaga agcaccgtta aaaaaggcga taattccgta acaatcaagc
     5221 ccattaagat gtgtccgata aactgggtca gcccgagctc tttgagcagg gtgaagacaa
     5281 cgccgataaa aagcgagccg aagagaattg aaacaataag aaaaagcggc aagatctgca
     5341 cggaagtgaa gtagagctga ttcaaaagga cttcccgcat ggcgcttgaa taggtttttt
     5401 gttgaaaaat ccgcagaaaa actttcccgg aaaacgcgat cgtcccggca aagccgctga
     5461 tcatccggac gacctgattc cctaattttt ctaacaagga catatatgtt cgctccgtga
     5521 tgcggcatcc taacacagga caaaaaatac ttcaaaccat cattataaat gcgttttacg
     5581 ttgacaaacg gaggcgtgtc gttatatttg aaataattat gcgttcaaaa acatccgaaa
     5641 agcagcacag gacgcattct tttggaggaa aagatgaaaa attcaaaccg aaaaacgttt
     5701 tttatgttcc tgatggcctt tctggtggcc ttttttattg tttcactggt gggcgttttg
     5761 agatcatcct ttaccagctc cggggctcct gaaattccca ccgcccaggc tgtccccagc
     5821 ctttccaaca cccccggcac ggctccgaat tctttttccg atctggtgga ccgtgttaag
     5881 ccggcagttg tcaatataag cacttccaaa acctataaag gaagaggcgg ttttggaaca
     5941 cctttcggag aatcgccttt cggggatgat ttctttgacc gctttttcgg cgacatgccc
     6001 cgcagagaat ttaaacagcg cagcctggga tcaggattta tcatcagcaa tgacggttat
     6061 atcttcacca ataatcacgt ggtggaacag gcggataaaa tccgcgtcaa aatatccgac
     6121 ggcaaggaat acgatgccaa agtcatcgga acggacgtca acacagacat agccttgatt
     6181 aaaatcaaac cgaccaacag cctgccggtt gcggatatcg gagattctga aaaagttcga
     6241 gtcggggaat gggtcgttgc gatcggcaat cctttcggtt tggatgcgac ggtaaccgca
     6301 ggaattgtca gcgccaaggg acgggtcatc ggcgccggac cttacgataa ctttattcaa
     6361 acagacgcct cgatcaatcc cggcaacagc ggcggcccgc tctttaatat ggaggggaaa
     6421 gtcatcggta ttaatacggc catcgtggcg cacggccagg gaatcggctt tgccattccc
     6481 atcaatatgg ccaaaaacat tctcgcggat ctgaaaacaa aaggaaaagt tacgcgcggc
     6541 tggctgggaa tatccgtgca ggatattacc gacgatgtcg cccaaaattt aaaccataaa
     6601 aacaaaaacg gcgcattggt atcggatgtt ttcaaaggcg atccggcaga tgcagcagga
     6661 attaaaattg gtgatatcat tatagaaatc aatgggaaac ccgtcaagga tacgcacgag
     6721 ctgctgctga tgatcgctgc catgaaagtc ggtcagaaga tgagcgtcaa agccattcgc
     6781 gacggaaagg aaatgatgtt ccaagtggta gtggcggagc gtaaagacaa tgtaacaatg
     6841 gccatggaaa ggtccgggct tggacatttt ggcattgccg cgcaggacat cactccggag
     6901 atggcaaagc agctcggaat cgcgcgcgat ggcgttatgg tcactgacgt tcaggcggga
     6961 agtccggccg acgaagtcgg cattcaaccc cgggacatca tcgttcaggt aaaccgggtt
     7021 aaaatatcat ccatgaaaga ttatacccgt gaaatcacca aagccgcgca aaagaaaagt
     7081 gtcacactgt tcatcaagag gggtcgatcc ggttattccg taacgatccg catcgattaa
     7141 agatccgtcc ttcggaaaaa ggcaggcctt cgggcctgcc ttttttatag aaatcaaacc
     7201 ctgaaaagag aaggccatgg acagaaagct tcatgattac ggtggtgtaa tccatctgca
     7261 ctcctccttt tcctttgacg gccacgcgcc gatggaagaa attctcgccg cagccgaaaa
     7321 gaatgaggtt gacttcatca tgctgacgga ccacgaccat ctgaaggcca gacaggaagg
     7381 atgggaagga tggcagaaaa aaacgctggt cctggtcgga gaagaaatag cccctagatt
     7441 taatcatttt ctggcgttta atatccagga gccggttata tgctcaggag accctgaagg
     7501 cataaatccg caaaaatata ttgatgaagt gaaccgtcag ggcggttttg gctttatcgc
     7561 ccaccccgat cacgaaggaa cgaaattgtt tcacgtgaaa cattacagtt ggaacaattg
     7621 gaatgtcggg gattttgcag gcatcggcgt ctgggatttt atgacggact ggcagaaaag
     7681 ccttcacgga tacggcagtg cgctcttgag cttccttttc cccgccttag ttttaaaagg
     7741 cccccgcagg attacgctgg aaagatggga cgccctgaac caaatccgga aaaccgtcgg
     7801 catcggggag ctggacaacc atgccaccgt gaaaaagctg ctgggaatcc gtttcgtcgc
     7861 cttccctttc caccgggcct tcgcgttcat ccgaacgcat gttttaacca aaaccggttt
     7921 caccggaaac aatcaacagg acatcgccat gttgtatgag tcgcttcttc atggacgctg
     7981 ctattttgcc ctggaatatt tccgcagggc aaaaggtttt caatttgctg ttgaacagga
     8041 tcagaaagta tttcatatgg gagattctct tccgctttcg ctttcaggaa acgctcaatt
     8101 atccgtctcc tgccccgcga aagctcgtat tcgtgtcatc agagacggcc gggaggaatg
     8161 ccgggcaatc ggcaatcggt taagcataaa aattgacaaa acaggggttt atcgcgtcga
     8221 agcgcacctg aagacggccg gaaaaacccg gccttggatt ttttccaacc cgatctttat
     8281 aaactctccg aaccattagc cttttgccac cgggaatacc gaaacaacat ggacaaagat
     8341 ttcaccgctc tttccgggct gggaaaaaca atacctttgt atttctgacc ttcgatttcg
     8401 atgaccgttc gggtcgtttc atcggtgagc agataaacgc cgatcaccgg cttgtgatat
     8461 tttcccataa cccggacggt tttttccaca agtcccctct cgtaaactct gagcagttcg
     8521 agattctcgg ccaccatttt cgggtcatag gacttgtcca ctgcaaccgt cgattccaaa
     8581 accgattgaa tcgtcacttt tctgccgata atgcccatat atataatcgc gtcgcattct
     8641 ttccactgca gcagctcttc gacaatcgcc acgtgggtgt ccgtattcat ttccgccacc
     8701 aaatccacgg gattggcatg gctccagtac gcaggaagca aaccattgat tttttgaatg
     8761 atttctgttg acagtggctg cacgatcagg ccgttctcgg cgcataggtc cgaagcgatg
     8821 acgccccacc ctcctcccag ggtcaggatg gccacccgat ttccgtgagg cagcggaaga
     8881 gaggaaaaag cggcggataa atcaagtaat tccatcgact ggttggcctg aatgattcct
     8941 gcctgtttgc aggctgcatt aaaaaccctg atattggatg ccattgctcc ggtatggctt
     9001 gccgcagcat gcgcccccgc ctgtgtgcgc cctcccttta aggcaatgat cggtttattc
     9061 cgaccgaccc tgcgcgccga ctcaaaaaat cttctgccat ttttgatgct ctcgatatac
     9121 aggactactg tctttgtcat cccgtccact tcgaaaccat ccagatagtc ctcaatcgtg
     9181 atcatcgcct cattgccgga gccgctgaac gcgcggatgc cgatgccctc tttttcggca
     9241 aacgcaagca gctgcgttcc cagattaccg gactgcgcaa ccagaaccgt gtctcccggt
     9301 tttggccgaa catgtgtccc cgtacaataa agagagcaat gcggattgca tatccccatg
     9361 gtattggggc caagaatcaa aacaccggcc tcgcgcgctt ttttaacgag ttcctcctcg
     9421 agggcttttc cttcagcccc cgtttctcca aatccggatg aaatgagcaa cgcattttta
     9481 atgcctttct gctgcaattc gtctattatc ggcagcactt tcccggcggg aatggtaatg
     9541 acggccagat cgaccggacc gggaatttcc gcaacggttt taaaggcttt tcttccggca
     9601 atttctccgc ctttgggatt gaccagatat actttcccgg aataacggcc cgcgatcaca
     9661 ttggtgtaca ttagctgccc ccacttgctc aattcagcgg aagctccgat aaaggcaatg
     9721 gatccgggat agaacagctt tgcgatcgct atcggttcca cgggaacatg tgtggtcttc
     9781 tgcaggctgc gttctccgag aacaatcaac gcatcgaccg ccgttacccg gccgtcgggc
     9841 gacacgagca ggggattgat gtcgatttcc ttgatttcag ggatgctcac ggccatttcg
     9901 gacagacctg ttagaacccg gaccagggcg tttctgtccg gggctttttc tccacggaaa
     9961 tcgctcagca gctttttggc gctcacctcc gaaatcatcc ggtttgcctc ttcttcgtca
    10021 atgggcgcca gccggaatac cacatctcca atggcctccg taaaaatccc ccctagaccg
    10081 aacatgatga ccggaccgaa ctgctcgtca aaaaacagac cggcgacaaa ctctcttttc
    10141 ccctccagca tgggctgaag taaaaaccct tccaaatcat ccccggccgc atcctttata
    10201 tatacagcgg ccgcctggag atcatccgga ttcttgatgt tgagtttgac cacacccctt
    10261 tccgttttat gggttagcct gctgcccaga ccttttaaaa caaccggaaa gcccatttct
    10321 tgggcggata cgattatgtc attgatatta tttattaatt tttcttctac cacgggaaca
    10381 tgatagtgct tcaacacaac ctttgcctca gcttcggtca atgcggacct gccttcttcg
    10441 atcgcttttg ttatgatttc tgacggattc ataggtaccc cttttcaata ttgactgtca
    10501 cgaagaaata gaatttccga ttttacagca taaccattgt ccactttttc tgttcctccg
    10561 gatcaactcc ctcgcttccg tcgtatcagt cctctccttc ggcggctgac tatccaccgg
    10621 agagacgact tgccttcaaa ataaaaactc ggggttcaat tttgaagaac caaacggagg
    10681 attttctgat agaaatcttt ttcccgctcc aggagattct ccagaatggg cgtggctggg
    10741 gagaagttcg gtgtatcctc ttcgctgatc cgccggaaga cggctgccac ttcgtgataa
    10801 ataccctcaa tgacagccat ctcacgggaa agacgaagcc tctggagcgc agggtattgt
    10861 gcttcttgct cctcaagaaa ctgccgatat agggtgcgaa atccggcgcc ccccgttccc
    10921 cgccgttcga tgacttggta tgtaaagcgc gcggaccacc gccagtcttc cgcttctttc
    10981 cagttggcca gatccctgat gagcgcttcc aacgatttga ctccataagc cgcgggcagc
    11041 cccggtatgc cgttgaggtt ctcctcggcc catagacgga tggcgctgtg caccacggtt
    11101 tcgtcaatgc ggaaccccga gatatcctca agcacgaagg ctttattcct cagcaaaaag
    11161 ggcgggaaag ccgcgctccg cgcacgctcc atgtctgtga tcgttacagc gagtgtttcc
    11221 ggatggagcg tgtccgaaac gtaaaattct tcttttttgt catcgtagcc acagatgacc
    11281 acggcgtggc cgggaaagtg ctgatcggtg ttgaagtagg gaaggtaggc gatgtcggtc
    11341 tgaacaagaa ccggcaagcc acagtccacc gcctctttag cgtgccgtgt cgcctccgcg
    11401 gcgttttcgg tctcccgcca acggaatttc agacctaccg cccgaaagaa gttctcctcc
    11461 aaggcaagac aacgcatgtg gactagccgg gaaggggttt gattaaggac gaggtagaag
    11521 aatcccggtc ctctccccaa cccgatgact gtgggttcgc tgatcttaat cccatgaaat
    11581 tccaatacat ttcggatggc gcttgacgca cagtggaatc ccggttgatg acggtatccc
    11641 ggaataatct gattctcgta catgacgacc ttcctgccgg gaattcattt tcaacttgta
    11701 aatcttcgtt gaaggcatcg ttttgaaaat gcatcgctct ctccgcctgg ggaaagggcc
    11761 gcactcatca atcaacacgg ttcccggctc gctcattctc gctgttctcc atttttacaa
    11821 atggaacctt atctattttt gaaatattgt caactgaaaa acccggggac gaacctcctt
    11881 gaatttacgg ccatcattac cctctgccgg aataatgggt cggcgtaagg atgccgaaat
    11941 gccgccgctc tttcaggcga taattttctc agaaaaaagg cgtgggaaaa gaatgcaggg
    12001 gcgcgtccaa atatggcctt ctgaaacggc gcgcggcagc gccgaagcgg atcgatcctt
    12061 cccggtccag tgtcaatatt tgctgttaat atttgcttga cgtatcctta atgtaatata
    12121 gtcttgggtc aaatctgctg ccgcgatttt agggctcgcg ctgatcgggg atcacatgac
    12181 caatccttcc gtaaatcaag taaacgttga cggtcgcctg atcggcatcg ttgatctgga
    12241 agaagcttta caaaaagcaa tttccgtctg cagtggaaaa agtgatttgg aaatttccga
    12301 ctttctcgtc cgggaggtct ccggccggaa ctacattcct tcgtcaatcc ttccggccta
    12361 cgcccgtgcc ctgctgcgcg aatacaaagc ggcgctgaac cttcccgtcg atcccgagcc
    12421 ggcaaccggc ctgactgttc ttgtgctggg cgcgggctgc gcccgttgcg atcaattgca
    12481 atcggacatc cgcgatgttt tatcacagat gcagatcgcc gcagatgtcc ggcatgtgac
    12541 cgacatccgg gaaatgtcca ggttcggcgt ccttggcgcg ccggcgctgg tgatcaacaa
    12601 cagggtggcg gccgtgggtg aagttccgcc caaaagtctc atccgccagt ggattaccga
    12661 cgctttgaaa aacggcgatt gagtttcctt cctcgcaccc gacccttcac gcacctcatc
    12721 tcttttgatc c
//
LOCUS       DAOSRM010000063        12334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5142_length_12334_cov_104.306214, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000063 DAOSRM010000000
VERSION     DAOSRM010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12334
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5142_length_12334_cov_104.306214"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="PLA58_07105"
     CDS             complement(<1..1757)
                     /locus_tag="PLA58_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOR62601.1"
                     /translation="MKQYFEKMDPLGKPLSAKQVESMRENCAKIEEIMKTIDAEVERI
                     RNVGVPLQTLHDRGEMSVWERIEYLVDPGTFCPLHSIFDPENEESGSTGVVDGLARIR
                     GKWCVLIGFNNKWMAGAWIAGQPDNNLRVTDIAKLLHLPLVWLVNCSGVKLPEQEKMY
                     ANRRGQGTCFFRHAELEQMGIPVIAGIYGTNPAGGGYQSISPTILLAHKKANMAVGGG
                     GIVSGMSPKGFFDEEGAEELINATRHFKSVPPGSVKIHHDVTGFFRAVFDEETQVLDA
                     IKDYMDDLPAYHPRFFRVAAPAEPAYPPSEIASIIPVNKKRVYDFEQVLARLVDGSEH
                     LEFRPGFGPEMYCGLVKVDGYLLGVVGNRQGAFPNYPEYTNQYMGVGGKLYRQGLIKM
                     SEFVTLCSRDKVPMAWFQDTSGIDVGDIAEKAELLGLGQSLIYSIENSHLPMMLVVLR
                     KGTAAAHYVLGGPTANNNNAFSLGTATTEINVMHGETAAAASYARRLVKEKDAGRPLG
                     PVIDKMNEMVKHYDDTSGPLFCAKKGFVDEIVRFHELRNYLVGFANCCYQNPRSICPQ
                     HQMILPRIIRSQMVKGLERP"
     gene            complement(1770..2927)
                     /locus_tag="PLA58_07110"
     CDS             complement(1770..2927)
                     /locus_tag="PLA58_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR62602.1"
                     /translation="MDFALTEEQKMIQETARNFAEKEIAPHVEEDEKNHFWRREIFDK
                     MAELGFFGFCIDEQYGGNGMGFLEGALAIEQVARVHVAWRMAFNMQCWGPALTIQKFG
                     TEEQKEHYIPKFVSGEYVGSFAMTEPDIGSDVASMKCRAEDKGDYYLVNGNKMWITNG
                     TVCNHGLLYVKTDKDAGANGVSCFIMDYSLPGITRKPIHDKVGLWASDTAEITFEDVK
                     VPKNLLLGKLNKGFQICMTQLNSTRLGCSAGALGLSGAILDASAKYATERCQFGKQIG
                     RYQLIQQQVADMKIGHDTLAYLVYRSAWLKDKGLPNVMETSMSKLYGAKVVVHDANEC
                     MKLYGSFGYSDEYPCGRFLRDSKQFETLEGTSNMHTMIVANATMGFAPNRA"
     gene            complement(3162..4427)
                     /locus_tag="PLA58_07115"
     CDS             complement(3162..4427)
                     /locus_tag="PLA58_07115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOR62603.1"
                     /translation="MENVVFISCARTPIGAYGGALREVPIYRLASIVLQAAVKKAGIE
                     PAQVDDVIMASAYQNGECANGARMAVLDADWPDTIPGGMIDRRCCSGLESVYYGALQI
                     QTGNADMVIAGGMESMSQGELYIPGDIKWGLGGKNHEKYGFMPRGHGALAMWGIPFYD
                     RIQRGRVMSQPIWRYGELSSMMTWAEAAAKKEGISREEADRWALRSHKRAIAAQEAGK
                     FTDEIAPVLTGKDKNGQEIFFSVDEGPRKDSSLERLAKLKPVYKDGVCTAGNSSSEND
                     GAAVVILASEKKARELGIKPIAAYRSCAVAATDPTLTYPAVPAAVAKALGKIGKTISD
                     MDLIEVQEAFAVQCLADSRLAGLSDEQMDAKVNINGSGISLGHPIGATGTMRLTTLLY
                     EMQRSNARFALETICGGGGQGICMVIERR"
     gene            complement(4477..6144)
                     /locus_tag="PLA58_07120"
     CDS             complement(4477..6144)
                     /locus_tag="PLA58_07120"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HOR62604.1"
                     /translation="MPTFDDRSGGFPRGLELLHDPGLNKGTAFTAAEREALGLQGLLP
                     PRISTQQQQVDHVIYNVRRKPNPLEKYLYLIGLQDRNEQLFYKALVDHLEELSPLVYT
                     PTVGLACQEYSHLYRRPRGITLTRYDRGRIKEALLNWPHRKVRVIVVTDGERILGLGD
                     LGSNGMGIPVGKLSLYTACAGIDPAACLPVMIDVGTNNNELWSDPQYIGLPEPRLEGE
                     AYDALIDEFMEAAEEAFPKVMIQLEDFGNRNAFRLLDRYRDRYCMFDDDIQGTAGVTL
                     AGLYSALRIIKGKITEQRIVFLGAGEAALGIGNLVASAMIRQGLAEEEALGRCWFVDS
                     KGLIVAGREDLNLHKRRFAHDFPFHRTLAEVVEAVRPTTLIGASGQPAAFGPEILEAM
                     ARLNERPIIFALSNPTSRAECTAEDAYRHTQGRAVFAGGSPFPKVEMNGQTFVPGQAN
                     NVYIFPGVGLGVMASDATRVTDEMFAAAAKCLDEQVSKDDLEQGRIFPSLARIRDVSA
                     AIALAVAKVAFAGKLTAMKEPSNLPGYIKSLMYDPVYSSYVPVKKHG"
     gene            complement(6185..7585)
                     /locus_tag="PLA58_07125"
     CDS             complement(6185..7585)
                     /locus_tag="PLA58_07125"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOR62605.1"
                     /translation="MTNQKTTAHPVRIQDNTFRDGHQSIYATRMKTEDMIPIAEEMDN
                     CGFWAMEVWGGATFDAMHRFLNEDPWARPRILKKYVRKTPFTMLLRGQNLVGYRHYAD
                     DVVRAFVDKSCEIGIDIFRCFDALNDFRNIETCVERIKANGKHMEGTVCYSLTESHLG
                     GEIYNLDFYVKKAGELEQMGADTICIKDMAGLLSPYDAFNLVTAMKKAVKVPIHLHTH
                     YTSGMGSMTYLKAIEAGVDIIDTCLAPYALRTSMPAIEPIVAALQGSDRDTGINLYKL
                     LEMSEHLEKIAPKYQHLLADQRLSIIDNGVLAHQIPGGMISNLTSQLKEMKALDRLGE
                     IYKEVVQTRKDMGYPPLVTPSSQIIGSQAVLNVLFGRYVRISNQLKDLVLGLYGRTPV
                     PIDPELTAKILKNYKRGQTPITGRPADHLPPEMEEAKKTIGDLARTDEDLLGWILFPQ
                     TMEKYLRAKYGIDAPA"
     gene            8095..10296
                     /gene="scpA"
                     /locus_tag="PLA58_07130"
     CDS             8095..10296
                     /gene="scpA"
                     /locus_tag="PLA58_07130"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOR62606.1"
                     /translation="MRVGKNTVGTVAADPCFLSKELNVTVHKNMKQWKEAALRELKGR
                     PLESLNWATPEGIVVKPIYTAEDLAGLDTVNTLSGLAPYLRGPSATMYANQPWTIRQY
                     AGFATAKESNAFYRKNLAAGQTGLSVAFDLATHRGYDSDHPRVAGDVGKAGVAIDSVE
                     DMKILFDRIPLDKVSVSMTMNGAVLPILAGYIVAAEEQGVAQKQLAGTIQNDILKEFL
                     TRNTYIYPPRPSMRIVSDIIAYCSKNMPRYNTVSISGYHIMEAGADSVLQTAFTLADG
                     KEYIRAAIDGGLKIDDFAPRLSFFFGVGMNFFMEIAMLRAARYLWHQIVGEFHPANPR
                     SQVLRTHVQTSGWSLTQQDPYNNIIRTTLEALAAALGGTQSLHTNSFDEAVSLPTPLS
                     SRIARNTQIIIQEESQICRVVDPLGGSYYVEALTASIIREAKKIMDEIEELGGMARAI
                     ETGMPKMRIEESAARRQARIDQGKDTIVGVNKYRIQDEVPVEVLDIPASVRDEQIARL
                     RDIKARRDAAAVQKALERITQVAEKGGNLLDASIDAVRLRATVGEVSDAMEKVFGRYV
                     ATTRVISGAYSSEYGDDEVIRSLRARTEKFLEKNGRRPRQLVTKMGQDGHDRGIKVVA
                     TAYADVGFDVDISPMFQTPEEAAKMAIENDVHVVGVSSLAAGHKTLVPELIEQLKKMG
                     GQDIIVVVGGVIPPADYDFLYQKGVKGIFGPGTAVTDSADRVLQLLEEKYG"
     gene            complement(10400..12235)
                     /locus_tag="PLA58_07135"
     CDS             complement(10400..12235)
                     /locus_tag="PLA58_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOR62607.1"
                     /translation="MEFKYDVRDLKFILKEWLPTEEVLACDRYKDNFSMDDIDLLLNE
                     GYKVAREVVNPINAPGDKTGVKLEKGVVTPVPGFKEAYQFLQQNGWGSSSECIKVDTG
                     MPLILYKACAEMNTAACPALTSCIKLTSGAANLILRFGTQEDRDRFVPKMLDGSWQGT
                     MCLTEPNFGSDTGDIITRSYPTDDPRIWKIKGTKMFITAGDQGTGENTIHLLLARPEG
                     GAPGSPGIGLYIVPKYWVNEDGSPGESNDVTTAGLEHKMGLHAQATALLNFGDDDNCR
                     GIMLGPAPDARGCSQGLAMMFHMMNESRIGTGHNANTQGAAACYFASQYATERIQGRP
                     FGIKNAERVAIIKHEDIRRMLLDMKAHVEGIRAMIFRGFYYLDIESNSSDREKAKKYS
                     SFAEILTPIVKCYGSEASLGVIAQAIQVLGGVGYTLDYPVEQYLRDSKILTIWEGTSF
                     IHANDLIGRKMRMKEGAPFAAWMQDIGNFIEVHQNEKGFEKEMKNLAAAFAAAEEVRA
                     IYNEWWADMEHKRRLIPLYAQRALFVFAYVYVAMCLLDQAFVAAKKSQDLKPHEGEYA
                     FYAGKIASARYYANNILPAAPMLASLIKSEEDTVLTCPEEALVVN"
BASE COUNT         2348 a         3570 c         3651 g         2765 t
ORIGIN      
        1 ggtctttcca gcccctttac catctgcgag cggatgatgc gcgggaggat catctggtgc
       61 tgcgggcaga tggaccgggg attctgatag cagcagttgg caaagcccac caggtagttg
      121 cgcagttcgt gaaagcgcac gatttcatcg acaaagcctt tcttcgcgca gaacagcggc
      181 cccgacgtat cgtcatagtg ttttaccatc tcgttcatct tgtcgatgac gggcccaagc
      241 ggccggcccg cgtccttttc cttgacgagc cttctggcgt aactggccgc cgcggcggtt
      301 tccccgtgca tgacgttaat ctcggtcgtg gccgtgccga gtgaaaaggc attgttattg
      361 ttggccgtgg gaccgcccag aacgtaatgc gccgccgctg ttcccttgcg caggacaacc
      421 agcatcatcg gcaggtgcga gttttcaatc gaatagatga gcgactggcc caggcccaga
      481 agctccgcct tctccgcgat gtcgcccacg tcgatgccgg acgtgtcctg aaaccaggcc
      541 atcggcacct tgtcgcgcga gcacagcgtg acgaactcac tcattttgat cagtccctgg
      601 cggtagagct tgccgcccac gcccatgtac tgattggtgt attccgggta attgggaaag
      661 gcgccctggc ggttcccgac gacgcccagc agataaccgt ccaccttgac caggccgcag
      721 tacatttccg ggccgaagcc ggggcggaat tccagatgct ccgagccgtc cacgagcctc
      781 gccagaacct gctcgaaatc gtaaacgcgc tttttgttga ccggaatgat cgaggcgatt
      841 tccgagggag ggtaagcagg ttcagcgggg gctgccacgc gaaagaagcg tggatggtag
      901 gcgggcagat cgtccatgta atctttaatt gcgtccaaga cctgggtttc ctcgtcgaag
      961 accgcgcgga aaaatcccgt gacgtcgtgg tggattttga cgctgccggg cggaacggac
     1021 ttgaagtgcc gcgtggcgtt gatgagctct tccgcgcctt cttcatcgaa aaatcccttg
     1081 ggggacatgc cgctcacgat gccgccgccg cccacggcca tgttggcctt cttgtgcgcg
     1141 agcagaatcg tcgggctgat ggactggtag ccgccgcccg cgggattggt gccgtaaatg
     1201 ccggcgatga ccgggatgcc catctgttcc agttcggcat ggcggaagaa gcacgtgccc
     1261 tggccccggc ggttggcgta catcttttcc tgctcgggca gcttcacccc ggaacagttc
     1321 accagccaca ccagcggcag gtgcaggagc ttggcgatgt cggttacacg caggttgttg
     1381 tccggctggc cggcgatcca cgcgcccgcc atccatttgt tgttgaagcc gatgaggacg
     1441 caccacttgc ctcggatgcg cgccagacca tcgaccacgc cggtgctccc cgattcctcg
     1501 ttttccggat cgaagatgct gtgcagcggg cagaaggtcc ccggatcgac gaggtattcg
     1561 atgcgctccc agacgctcat ctcgccccgg tcgtgcaggg tctgcaaggg gacgcccacg
     1621 ttcctgatcc gctcgacttc cgcgtcgatg gttttcatga tctcctcgat cttcgcgcag
     1681 ttctcccgca tgctttcgac ctgtttggcg gaaaggggct tccccaacgg atccattttt
     1741 tcgaagtatt gtttcatggc ttactccgtc tatgcccggt tgggcgcaaa gcccatcgtg
     1801 gcgttggcga cgatcatcgt gtgcatgttg gacgtgcctt ccagcgtttc gaactgcttg
     1861 gagtcgcgca ggaagcggcc gcagggatat tcgtccgaat agccgaacga cccgtagagc
     1921 ttcatgcact cgttggcgtc gtgaacgacg actttcgcgc cgtagagctt ggacatggag
     1981 gtttccatga cattgggaag ccctttgtcc ttgagccagg ccgagcggta aaccagatag
     2041 gccagcgtgt catggccgat tttcatgtcc gccacctgct gctggatgag ctggtagcgg
     2101 ccgatttgct tgccgaactg gcagcgttcc gtcgcgtatt tggcggaggc gtcgagaatg
     2161 gcgccggaaa gccccagcgc gcccgcggag cagcccagcc gggtggagtt gagctgcgtc
     2221 atgcaaatct ggaagccttt attcagtttt cccagcaaaa gatttttggg aaccttcacg
     2281 tcttcaaagg tgatttccgc cgtatcggag gcccacaggc cgaccttgtc gtgaatgggt
     2341 ttgcgcgtga tgccgggcag ggagtagtcc atgatgaagc agctcacgcc gttcgcgccc
     2401 gcgtccttgt ccgttttgac atacaacagg ccgtggttgc aaaccgtgcc gttcgtgatc
     2461 cacattttgt tgccgttcac caggtagtag tcgcccttgt cttcggcgcg gcatttcatg
     2521 gaggcgacgt ccgaaccgat atccggttcg gtcatggcga agctgccgac atattcgccg
     2581 gagacgaatt tgggaatgta atgttctttt tgctcttccg ttccaaattt ctggatggtc
     2641 agcgccggac cccagcactg catgttgaag gccatgcgcc aggcgacatg caccctggcc
     2701 acctgttcga tggcgagcgc gccttccaga aagcccatgc cgtttccgcc gtattgctcg
     2761 tcgatacaga agccgaaaaa tccgagctct gccattttgt caaaaatttc cctgcgccag
     2821 aaatggtttt tttcgtcttc ctcgacatgc ggcgcgattt ccttttcggc aaagtttcgc
     2881 gccgtctcct gaatcatttt ttgttcttcc gtcaatgcaa aatccatatt ttttcctccc
     2941 ggacggttgt taaaggttgt tgactttgtc acaccggtga aaaccggtgt ccaggaatga
     3001 atccattttt caatccactg gattccggct ttcgccggaa tgataattta gggaacagtc
     3061 tcgtgatctc gcgtgccctc cagggtatca ggttatcgcg acggtttccc gctgctattt
     3121 tagatttgcg gcaatccgta gggaacggtc gcgaccgttc cttaccttct ttcgatcacc
     3181 atgcatatcc cctggccgcc gccgccgcag atcgtttcca gggcgaagcg ggcgttgctc
     3241 cgctgcattt cataaagcag cgtggtcagg cgcatcgttc ccgtggcgcc gatggggtgc
     3301 cccagcgaga tgcccgaacc gttgatattg accttcgcgt ccatctgctc gtcggaaagt
     3361 cccgccagac gggagtccgc cagacactgc acggcaaacg cctcctggac ttcaattaaa
     3421 tccatatcgg aaatggtctt gccgattttc cccagcgcct ttgcaacggc tgccgggacg
     3481 gcagggtagg taagggtcgg atcggtggcc gcgaccgcgc aggagcggta ggcggcgatc
     3541 ggtttgattc ccagttctct ggctttcttt tcggaagcaa ggatgaccac ggccgcgccg
     3601 tcgttttccg aggaggagtt gcccgccgtg caaacgccgt ctttgtaaac gggttttagt
     3661 ttggccagac gttccaggga tgaatctttg cgggggcctt cgtcgacgct gaaaaaaatc
     3721 tcctgaccgt ttttgtcctt gccggtgaga accggagcga tttcgtccgt gaacttgccg
     3781 gcttcctgcg ccgcgatcgc ccgcttgtgg gatcgaagcg cccagcggtc cgcttcttcg
     3841 cgcgaaatgc cttctttttt ggcagcggcc tccgcccagg tcatcatgga gctgagctcg
     3901 ccgtagcgcc agatgggctg gctcatgacg cgtccgcgtt ggatgcggtc gtaaaaggga
     3961 atgccccaca tggcgagcgc cccgtggccg cgcggcatga agccgtattt ctcatggttc
     4021 ttgccgccca gcccccattt gatgtctccg gggatgtaga gttcgccctg gctcatcgat
     4081 tccatgccgc ctgcgataac catatcggcg ttgccggttt gaatctgcaa cgccccgtaa
     4141 taaacgcttt ccaggccgga gcagcagcgc cggtcgatca tgccgccggg gatggtatcc
     4201 ggccagtcgg cgtccagaac ggccatgcgc gcgccgttgg cgcactcgcc gttctgatag
     4261 gcggacgcca tgatgacgtc atccacctgg gccggttcaa tgcccgcttt tttgacggcc
     4321 gcctgcagga caatgctggc cagacggtaa atcggaacct cccgaagcgc tccgccgtac
     4381 gcgccgatcg gcgtccgcgc gcagctgata aacacgacat tttccattgc ttttctccct
     4441 gtgcagtttt gtggatcata cctccgccgg ctttttttaa ccatgttttt ttaccggcac
     4501 atacgacgaa tagaccgggt catacatcag cgacttgata tagcccggca aattggaagg
     4561 ttccttcatc gcggtgagtt ttccggcaaa ggcgactttg gccacggcca gcgcaatggc
     4621 ggccgaaacg tcgcggatgc gcgcaagcga cggaaagatt cttccttgtt ccagatcatc
     4681 ctttgatacc tgttcatcca gacatttggc cgccgcggcg aacatttcgt ccgtcacacg
     4741 ggtcgcgtcc gaggccatga cgccgaggcc cacgccgggg aagatgtaaa cgttgttggc
     4801 ctgacccggc acaaacgttt gaccgttcat ctccaccttc ggaaacggac tgccgccggc
     4861 aaaaacggcg cgcccttgtg tgtggcggta agcgtcctcg gccgtgcatt ccgcccgtga
     4921 ggtcggattg gaaagcgcaa agataatcgg acgttcgttc agacgcgcca tggcctccag
     4981 aatttccggc ccaaaggcgg cgggctgccc cgacgcgccg atgagggttg tcggccggac
     5041 ggcttccacc acttccgcga gagtgcggtg aaacggaaaa tcatgggcaa agcggcgttt
     5101 gtgaaggttc agatcctcgc ggccggccac gatgagccct ttggaatcga caaaccagca
     5161 ccggccgagg gcttcttctt cggcaagccc ctgccggatc attgcggagg ccaccagatt
     5221 cccgatgccc agggcggctt ctcccgcgcc cagaaacaca atccgctgtt cggtaatttt
     5281 ccccttaatg atgcgcaaag cggagtagag cccggccagc gtgacgcccg cggtgccctg
     5341 aatgtcgtcg tcgaacatgc agtaccggtc gcggtagcgg tccagcaggc ggaaagcatt
     5401 ccggttgccg aagtcttcca actggatcat gaccttcggg aatgcctctt cggccgcttc
     5461 catgaattca tcgatcagcg catcgtatgc ttctccctcc agccggggtt ccggcaggcc
     5521 gatgtactgc ggatcgctcc acaattcgtt attgttggtg ccgacatcga tcatgacggg
     5581 caggcaggcc gccgggtcaa tacccgcgca ggccgtgtag agcgacagct ttcccacggg
     5641 aatgcccatg ccgttggatc ccaggtcgcc caggcccagg attctttcgc cgtccgtgac
     5701 gacaatgacc cggaccttcc ggtgcggcca gttgagcagc gcctctttga tgcggccccg
     5761 gtcgtagcgg gtcagcgtaa tgccgcgggg acgccggtac agatggctgt attcctggca
     5821 ggccagcccc acggtggggg tgtaaacgag ggggctcagt tcttccagat ggtccacgag
     5881 cgctttgtaa aaaagctgtt cgttgcggtc ctgcaggccg atcagatata ggtatttttc
     5941 cagaggattg ggtttccgcc ggacgttata gatcacatga tccacctgct gctgctgggt
     6001 cgagatgcgc ggcggcagca gaccctgcag gcccagcgct tcgcgttcgg ccgcggtaaa
     6061 agccgtccct ttgttcaggc ctggatcgtg gagcagttcc agcccccggg gaaatccgcc
     6121 cgaacggtca tcgaaagttg gcatcgtttt tatcctcacc ctatccatac tctcccgcga
     6181 ccgcctatgc cggcgcgtcg attccgtatt ttgcccgcag atatttttcc atggtctgag
     6241 ggaacaggat ccagcccagc agatcttcgt cggtgcgcgc cagatcgccg atcgtttttt
     6301 tcgcctcttc catttcgggg ggaaggtggt ctgccggcct gccggtgatc ggcgtctgtc
     6361 cgcgtttgta gtttttgagt attttcgcgg tcagctccgg atcaatcggc acgggggttc
     6421 tgccgtagag accgagcacg aggtctttga gctgattgga aatccggacg tagcgcccga
     6481 acaggacgtt cagcaccgcc tgcgagccga tgatctgcga agacggcgtc accagcggcg
     6541 gatagcccat gtcttttcgc gtctggacga cttctttgta gatttcaccc agccggtcga
     6601 gggccttcat ttccttgagc tggctggtca ggttggaaat catgccgccg ggaatctgat
     6661 gcgcgagcac gccgttgtcg atgatggaaa gtctctggtc cgccagcaga tgctggtatt
     6721 tgggggcgat cttttccagg tgttcgctca tctccaggag cttgtagagg ttgatgcctg
     6781 tgtcccggtc cgatccctga agcgccgcca caatgggttc gatggcgggc atggaggtcc
     6841 gaagcgcgta tggcgcaagg caggtgtcga tgatgtccac cccggcttcg atggctttca
     6901 gataggtcat gctgcccatg ccgctggtgt aatgcgtatg aagatggata ggaactttga
     6961 cggctttctt catggcggtg accaggttga aggcgtcgta gggggaaagc agaccggcca
     7021 tgtccttgat gcagatcgtg tccgcgccca tttgttcgag ttctccggct ttcttcacgt
     7081 agaaatcgag gttataaatc tccccgccca gatggctttc ggtgagggag tagcagacgg
     7141 ttccttccat gtgcttgccg ttggccttga tcctttccac gcaggtttcg atgttgcgga
     7201 aatcgttgag ggcgtcgaaa cagcggaaaa tgtcgatgcc gatctcgcag gatttatcga
     7261 cgaaagcgcg gaccacgtca tcggcgtaat gacggtaccc caccaggttt tgtccgcgca
     7321 gcagcatggt aaaaggggtt tttctgacgt attttttcag aatccggggt cttgcccacg
     7381 gatcttcgtt gaggaaccgg tgcatggcgt caaaggttgc gccgccccac acttccatgg
     7441 cccagaagcc acagttgtcc atttcttcgg caatcggaat catgtcctcg gttttcatcc
     7501 gggttgcgta aatggactga tggccgtcgc gaaacgtgtt gtcctgaatt ctgacggggt
     7561 gggcggttgt cttttggttt gtcatgcgga actccttgat gcagatgtgt cttgttatac
     7621 attacagcaa ataacatgcc agccattttc cgcttttggg gagtgaacgg cgacgccggg
     7681 tccgttcggc cttctttcgt tttcaatcct gcttcggcgt gacgagcgtt gattcttcgc
     7741 ggcttgctgc gataggatga cgttcacttc atgcttcgac aacttgccgc gaggtgtttg
     7801 attagatgtt atgtattgcg gcgcaaatgt gatatgcaac tattcggttt gccgcccgga
     7861 aggacatgag ctgtatatca tatatacggc ccgttgggta agggtatatt ttgtaaacat
     7921 attgctcgcc ggggaagcaa tccgcggtat tgtggacaca aaacgtgcgg ccgggcgcgt
     7981 ttgaaccgac gggaaataag cagagacgtt tttctccgaa ggaggagaaa aacaggccgg
     8041 acaaagcctt gcctgattgg aacgacttat gcatcctaag gcaaacagct tttcatgaga
     8101 gtaggcaaaa atacggtggg gacggttgca gccgatccct gctttttatc aaaggagttg
     8161 aacgtgaccg tgcacaagaa catgaaacaa tggaaagaag ccgccctccg ggagctgaag
     8221 ggaaggcccc tggagtcttt gaactgggcg acgccggagg gcattgtcgt caagccgatc
     8281 tacaccgcgg aagacctggc ggggctggac accgtcaaca ccctgtccgg ccttgccccg
     8341 tatctgaggg ggccttcggc caccatgtac gccaaccagc cctggacgat ccgccagtac
     8401 gcgggattcg ccacggccaa ggaatccaat gcattttacc ggaagaatct ggccgcgggg
     8461 cagacgggcc tgtccgtcgc ttttgatctg gcgactcatc ggggctacga ctccgaccat
     8521 ccgcgtgtgg cgggagacgt ggggaaggcg ggggttgcca tcgattccgt cgaggacatg
     8581 aaaatcctct tcgaccggat tccgctcgac aaggtgtccg tttccatgac gatgaacggc
     8641 gccgtcctgc cgatcctggc gggatacatc gtggccgccg aggagcaagg cgtggcgcaa
     8701 aagcagctcg ccggcaccat tcagaatgac atcctcaagg aatttctgac gcgcaacacc
     8761 tacatctatc cgccgcggcc ctccatgcgg attgtttccg acatcatcgc ctactgctcg
     8821 aaaaacatgc cgcgctacaa caccgtttcg atcagcggct atcacatcat ggaagcgggc
     8881 gcggattcgg tgctccagac ggcttttacg ctggccgacg gcaaggagta tatccgcgcc
     8941 gccatcgacg gcggcctgaa gattgatgat ttcgctccgc ggctttcgtt tttctttggg
     9001 gtgggcatga actttttcat ggaaatcgcc atgctgcgcg ctgctcggta tctgtggcat
     9061 caaattgtcg gagaatttca tccggccaat ccgcgatcgc aggttctgcg cacgcacgtg
     9121 cagacgtccg gctggagcct gacccagcag gacccctaca acaatatcat ccggaccacg
     9181 ctggaggcgc tggccgcggc gctgggcggc acgcagtcgc tgcacaccaa ttcgttcgac
     9241 gaggcggtaa gcctccccac gcctctgtct tcgcgcattg cccgcaacac gcagatcatc
     9301 attcaggagg aatcgcagat ctgccgcgtg gtcgatcccc tgggcggctc ctactacgtg
     9361 gaggcgctga cggcgtccat catccgcgaa gcgaaaaaaa tcatggacga aatcgaggag
     9421 ttgggcggca tggcccgcgc cattgaaacc ggcatgccca agatgcgcat cgaggagtcg
     9481 gccgcccggc ggcaggcccg catcgatcag ggaaaagaca ccatcgtcgg cgtgaacaag
     9541 taccggattc aggatgaagt tcccgtcgag gtgctcgaca ttcccgcgag cgtgcgcgac
     9601 gaacagatcg cgcgcctcag ggacatcaag gcccggcgtg acgccgccgc ggtgcaaaag
     9661 gccctggagc ggatcacgca ggtggccgaa aagggcggca acctgcttga cgcttccatc
     9721 gacgccgtgc ggctgcgggc cacggtggga gaggtttccg atgccatgga gaaagttttc
     9781 ggccggtatg tcgccaccac gcgggtgatt tccggcgcct attcatccga atacggagac
     9841 gacgaggtga tccggtcgct tcgggcgcgg acggagaaat tcctggaaaa gaacggccgc
     9901 cgcccccgcc agctggtgac caaaatgggg caggacggcc atgaccgggg gatcaaggtg
     9961 gtggccacgg cctatgcgga tgtcggcttt gatgtggaca tcagtccgat gttccagacg
    10021 ccggaagagg cggccaaaat ggccattgaa aacgacgtcc acgtggtggg tgtgtccagt
    10081 ctcgcggcgg gccacaagac gctggtcccg gagctcattg aacagttgaa gaagatgggc
    10141 ggccaggaca tcatcgtcgt ggtgggcggc gtcattccgc ctgcggatta cgatttcctc
    10201 taccagaagg gcgtcaaagg catcttcggc cccggcacgg ccgtcaccga ttcggcggac
    10261 cgggtgctgc agttgctgga agaaaaatac ggataacgac aaaacgggcg gccgcgaaaa
    10321 cggccgccag aaaaaaccag taaattcgat cccttgctgg agggtggcgg caggaaacga
    10381 tcgcgaccgt tccctgccgc taattgacaa ccagcgcctc ttccgggcac gtcaagacgg
    10441 tgtcttcctc gctcttgatg agtgacgcca gcatcggggc ggcgggcaga atgttgttgg
    10501 cataatagcg cgccgatgca atcttgcccg catagaaggc gtattctccc tcatggggtt
    10561 tcagatcctg cgacttcttt gccgcgacga aggcctgatc aagcaggcac atcgccacat
    10621 agacatacgc aaagacaaac agcgccctct gggcgtaaag cggaatgagc cggcgtttat
    10681 gctccatgtc ggcccaccat tcgttgtaaa tcgcccggac ctcctccgcg gcggcaaagg
    10741 cggcggcgag gttcttcatt tctttttcaa accctttttc gttttgatga acctcgatga
    10801 aattcccgat atcctgcatc caggcggcga acggtgcgcc ttctttcatc cgcattttcc
    10861 gtccgatgag gtcgttggca tggatgaacg atgttccctc ccagatcgtg agaatcttgg
    10921 aatcgcgcag atactgctcc accggatagt ccagcgtgta gcccacaccg cccagcacct
    10981 ggatcgcctg cgcgatgacg cccagcgaag cctcggagcc gtagcatttg acgatgggcg
    11041 tgaggatttc ggcaaacgaa gagtattttt tggccttttc cctgtcggag gagttgcttt
    11101 cgatgtccag gtagtaaaat ccccggaaga tcatggcccg gatcccttcc acatgcgctt
    11161 tcatgtccag aagcatccgg cggatgtctt cgtgtttgat gatggcgacc cgttcggcgt
    11221 ttttaatccc gaagggtctt ccctggatgc gctcggtggc gtactgggag gcaaagtaac
    11281 aggctgccgc gccctgcgtg ttggcgttgt gccccgtgcc gatgcgggat tcgttcatca
    11341 tgtggaacat catggccagg ccctgcgagc agcctctggc gtcgggagcg ggccccagca
    11401 taatgccgcg gcagttgtcg tcgtcgccga agttcagaag cgccgtcgcc tgggcgtgga
    11461 gccccatctt gtgctcgagg cccgctgtcg tgacgtcatt ggactctccc ggcgaaccgt
    11521 cttcattgac ccagtacttg ggaacgatgt acaggccgat gccgggcgat cccggcgctc
    11581 cgccttcggg gcgggccaga agcagatgaa tcgtattttc gcccgtgccc tggtcgccgg
    11641 cggtgataaa catcttcgtg cccttgattt tccagatgcg cgggtcgtcg gtcggatagg
    11701 agcgcgtgat gatgtcgccc gtatcggagc cgaagttggg ttcagtgagg cacatcgtgc
    11761 cctgccagga tccgtcgagc attttgggga caaaacggtc ccggtcttcc tgcgtgccga
    11821 agcgcaaaat caaattggcc gcgccggacg tgagtttgat gcaggaggtc agggccgggc
    11881 aggcggcggt attcatttcg gcgcatgcct tatagaggat gagcggcatg ccggtgtcca
    11941 ccttgatgca ctcgctcgac gaaccccagc cgttctgctg cagaaactga taagcttcct
    12001 taaaccccgg aaccggcgtt acgacgcctt tttcgagttt cacaccggtt ttgtcgcccg
    12061 gcgcgttgat gggattgacg acttcccggg cgaccttgta gccttcgttg agcagcaggt
    12121 cgatgtcatc cattgagaaa ttgtctttgt atcgatcaca ggccagcact tcttccgtcg
    12181 gcagccactc ctttaaaata aatttcaaat cccggacgtc gtatttgaat tccatttatt
    12241 tctccttgct catcacttct tttaccattc actactcacc atccaccatt cactatttgt
    12301 catggttcga ccagctcacc actcgacgag ctca
//
LOCUS       DAOSRM010000064        12268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5178_length_12268_cov_130.298207, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000064 DAOSRM010000000
VERSION     DAOSRM010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12268
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5178_length_12268_cov_130.298207"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..170
                     /locus_tag="PLA58_07140"
     tRNA            95..170
                     /locus_tag="PLA58_07140"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Ala,seq:tgc)
     gene            328..3996
                     /locus_tag="PLA58_07145"
     rRNA            328..3996
                     /locus_tag="PLA58_07145"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4084..4199
                     /gene="rrf"
                     /locus_tag="PLA58_07150"
     rRNA            4084..4199
                     /gene="rrf"
                     /locus_tag="PLA58_07150"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(4330..6012)
                     /locus_tag="PLA58_07155"
     CDS             complement(4330..6012)
                     /locus_tag="PLA58_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOR62608.1"
                     /translation="MIEKPWHRHYDYNVPTTIRYPRLAAHELLNLPTGAYPDKAALIF
                     FGSEMTFLELSIVSKSFANALAELGIKKGDRVGLHLPNCPQYLISYFAVLSLGGIVVN
                     LNPLYTPDELKLMADTSGMTSLITFDMALPAIRTLCKQVLISRVIVTAVTDYIKGFPV
                     SSAKSLDLEPGWRHFSELIAKHPNPKRVKVQVNPEDPALIQFTGGTTGVPKGAVLTHA
                     NIVAGTFQCSLWGNSTITLTAHEKRNVMAVLPYFHVYGNIVVMNWALFNCATQIQVPR
                     FNIDEMMDLLAKFDQITFFPTVPTLITALINHPKAAEMNLNKKFGLLNSGGAPMPVEL
                     IEQVKDMGIYFSEGYGLSESTSLGISNPVLGLKKVGSIGIPFPDNDVRLVDVDKGETD
                     VPDGEPGEIIMKGPLIMQGYWNNPAETEGQLKDGWLHTGDIAVRDEDGYLFIVDRKKD
                     MIIAGGFNIYPRDIDEVLFQHPKVAEAVSLGVKDQYRGETVKAFVVLKAGESCTAEEI
                     ISFCKEKLAPYKVPKLVEFRESIPKSAVGKILRKVLRDEEEARAKKDQQPGR"
     gene            6373..7167
                     /locus_tag="PLA58_07160"
     CDS             6373..7167
                     /locus_tag="PLA58_07160"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOR62609.1"
                     /translation="MMMNLSQFSLEGKVAVVTGGSRGIGRAAALAMADAGAHVVVSSR
                     KIADLEGVAEEISARGVKGRAIAAHVGKMDDSRALVDQVVKEFGRLDILVNNAGTNPY
                     YGPMMDQDEKTYDITMNVNVKSIFFLSQMAAKMMKTQGGGSIINTSSIGGIRAGELGV
                     YCATKAAVIMLTQVMAREWGQYGIRVNAIAPGIIKTRLSEALWKNPEVNARAVAQIPM
                     MRLGEPEELAGVVVFLASRAGSYVTGETIVIDGGRVHGEPAWREKK"
     gene            complement(7256..7822)
                     /locus_tag="PLA58_07165"
     CDS             complement(7256..7822)
                     /locus_tag="PLA58_07165"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HOR62610.1"
                     /translation="MDAAWKSQGKKLHTRNIEISTYECDENRIIVEGFLKDDRHQNTF
                     AVTGETFPAGVIHHMGIRLLVNCSTFLIEDIDVDLISVPRSVCRETIDSLAPIRGLTI
                     TRGFTSKVKKLVGGTKGCTHLLELLLAMAPAAVQGFAAHRSRKPYDFDPQQAKLILKF
                     LINTCHAWREDGPFVQSHKEMLQKALKL"
     gene            complement(7988..8302)
                     /locus_tag="PLA58_07170"
     CDS             complement(7988..8302)
                     /locus_tag="PLA58_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62611.1"
                     /translation="MKIVRTDLMPGRKLAAINLFGIVFAPKSGGLLQKTINHEAIHSA
                     QMKEMLFILFYLWYAVEWLVRLFGKGNAYRNIAFEKEAYAHEQDKYYLKRRRPYAWLK
                     RL"
     gene            complement(8299..9105)
                     /locus_tag="PLA58_07175"
     CDS             complement(8299..9105)
                     /locus_tag="PLA58_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOR62612.1"
                     /translation="MKIVRFVSKRKGPLYGVYDPRRPEVANPIEGDPWNPRSVGDREE
                     QIERLLAPVAPVNILALGINYRKHGEETALSAPSEPILFLKATSSVIGPGHPIELPAA
                     GPDQVDYEAELAVVIGKTAKNITPEEAPDYIFGYTCANDVSARDWQFERQKDQWARGK
                     SFDTFCPLGPWIVTKDEIDNPDDLAIRAVLNGKTVQKSRTSEMIFDVRTIVSRLSRSM
                     TLHPGTVILTGTPEGVGFTRRPPLFLQPGDDIRIEIEKIGTLSNRVIAES"
     gene            complement(9107..10399)
                     /locus_tag="PLA58_07180"
     CDS             complement(9107..10399)
                     /locus_tag="PLA58_07180"
                     /inference="COORDINATES: protein motif:HMM:NF017808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR62613.1"
                     /translation="MKRRLHDFRWRLAAALIMMTAVLTGCRYLSIPSQDDAVPSLLSL
                     KGEKGPPAYNSAVIKGRLTGPGWRGNSTLILACSPADKKNECAEYTATGLNQDTFMLY
                     LPEGRYRLFAVTDYNHNGTYEEDEASGMSGSGSQTLDIAVREGELISGVEIRTIATDE
                     QPVQLPRGWRRKIGEPIIRQATGNGDVLKIYHEYFSLENAQIGYWNPSSFMKMFGAHI
                     YLTQAYDPRKIPLLFVHGTEGSPHNWIYFYMRLDRSKYQPWFFYYPSGIRLNLASALL
                     DEELRELHEKFGFRKMALVAHSVGGLTTKAFLDRRRSEGQNTFVRLFVSLATPWSGFG
                     LADASQMLPHKSIPVWLDLGTQSDFISATMNTKLPSTTHYYLFYGKNDKLAGKTALDI
                     RAVSCAVRVYGFDCTHDGILIDRKVFFQFDSILEKEMF"
     gene            complement(10396..11487)
                     /locus_tag="PLA58_07185"
     CDS             complement(10396..11487)
                     /locus_tag="PLA58_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOR62614.1"
                     /translation="MRNAIVTLIMLFFIASIAACMPPPLQKKDEKVTAALADPQTSFF
                     EASRKGDIVLVMRLVEEKQIDVNQTDDYAWTGLMYAVQNGHVGLAEYFLLRKSQINRQ
                     DADGLTPLMIAANERHPDIVELLLAFGADPEAQDRHGYTALMWAIYNKSWDCIPPLLA
                     TGKGLDLQNGFGETALHLALDYRCSDIAAMLIRRHANVNLKDGRGRTPLHLAAKKGDH
                     DNVLLLLAEGADTAARDEAGENPLMKSIRNGRVSVARLLLDQTGNLQTANQEGQTALH
                     LAAAEDLPDIAAALIRRKADLNRPDRQGDTPLMLAVESGSKRTIILLAERGADLKAKN
                     KEGKTALDLAREKRLGNVVEYLERKMGKP"
     gene            11692..12072
                     /locus_tag="PLA58_07190"
     CDS             11692..12072
                     /locus_tag="PLA58_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62615.1"
                     /translation="MTDILDYISTWSAIKWVVLVLIAGFIGQFGRMMAEAIANRIRLR
                     RAKKNPTAAGRQETEETKTLNAGAVRESGLSGSVQETPGLPAGAPASDFPSRPWPSPI
                     APDKKALKTMAKARKKEAKREGKE"
BASE COUNT         2887 a         3065 c         3498 g         2818 t
ORIGIN      
        1 cgcacgcctg ataagcgtga ggtcggtagt tcaaatctac ctaggcccac cagttgaggg
       61 tgttgcgaca gacggtggtg aggattcggg accagggggt gtagctcagc tgggagagcg
      121 cctgccttgc acgcaggagg tcatcggttc gatcccgttc acctccacca gatatacaga
      181 ctgtgactga tcggtcatgc gacttgcgta tgaaacatca gtcatcggct gtatcgccgg
      241 tgagtttgtt ctttgacaat tgcatatcgt aaaatttttt caatttgttg aaaagctgag
      301 caaaatattt gttgaataat tatttatggt caagctacta agggcgcaag gtggatgcct
      361 tggtgccagg aggcgatgaa agacgtggtt agctgcgata agcttcgggg agccgctaaa
      421 caggcccaga tccggagatc tctgaatggg gaaacccggt ccgtgtcatg acggatcatc
      481 tgcaactgaa tacataggtt gcagaagcga acggggggaa ctgaaacatc taagtaccct
      541 caggaaaaga aaacgacagt gattccccaa gtagcggcga gcgaacgggg aacagcctaa
      601 accggcggtg cgtgatagcc tgcaagcgtt gtatcgccgg ggttgcggag ctccattgga
      661 gggaattgca gttctctcgg aaagttacaa aatctgttta tagccgaagc tgctggaaag
      721 cggcgtcaca gagggtgata gccccgtaag cgaaatgaac agatctttct ggatggagtt
      781 tccaagtact gcgggacacg agaaatcccg caggaaaccg gggggaccat ctcccaaggc
      841 taaatactac ctggcaaccg atagtgcact agtaccgtga gggaaaggtg aaaagtactc
      901 cgagaggaga gtgaaatagt acctgaaacc gtgcgcctac aaggtgtgga aggaccaggg
      961 atgagggctt gcccttatcc ggtctgactg cgtgcctttt gtataatgag tcagcgagtt
     1021 actctatgca gcgaggttaa gccgtcaggt ggagccgtag cgaaagcgag tcttaatagg
     1081 gcgatttagt tgcatggagt agacccgaag ccaggtgatc tattcatggc cagggtgaag
     1141 cgtatgtaac agtgcgtgga ggcccgaacc ggtgtgggtt gaaaaccgct cggatgagct
     1201 gtgaataggg gtgaaaggcc aatcaaactt ggaaatagct ggttctcccc gaaatatatt
     1261 ttggtatagc cttgcaactt taagtaccgg aggtagagca ctgaatgggc taggggtcct
     1321 accagactac caaccccaac caaactccga atgccgataa cttcaattgc aggagtcaga
     1381 ctgcgggaga taagttccgt ggtcgaaagg gaaacagccc agaccgccag ctaaggtccc
     1441 taatacatgc taagtggaaa aggatgtgga agcgtataga cagccaggag gttggcttag
     1501 aagcagccac ccttcaaaga aagcgtaata gctcactggt caaatgagtc tgcgccgaaa
     1561 atttaacggg gctcaagcat gtaaccgaag ctgcggactt gcatcgcaag atgggagtgg
     1621 taggggagca ttctgtcgta ggttgaagcc ggatcgtaag gcccggtgga cgaaacagaa
     1681 gagattatgc tgacataagt agcgataaag tctgtgaaaa acagactcgc cgaaaaccta
     1741 aggtttcctg agtaaagcta atcttctcag ggttagtcga tccctaagcc gaggccgaca
     1801 ggcgtaggtg atgggaaaca ggttaatatt cctgtaccac tgaagacgcg ttatcaacta
     1861 aggggggacg tagaagggta agccatccgg gtgttggatg tcccggttca agcaagtagg
     1921 aggagattcc aggcaaatcc ggaatcttat ttaaaactcc gagatgcgac gaggagagat
     1981 ttatctcata aactggccga tcccatgcta ccaagaaaat cctcgtagtg agtttctttg
     2041 gtgctcgtac cgtaaactga cacaggtagg taaggagaga atcctaaggc gcttgagtga
     2101 tctctcgtta aggaactcgg caaaatgaca ccgtaacttc ggaagaaggt gtgcctcgtt
     2161 agcgtgaagc gattcgcacg tgaagcgcga agaggttgca atgaaacggc ccaagcgact
     2221 gtttagcaaa aacacaggac tctgctaagt cgtaagacga tgtatagggt ctgacgcctg
     2281 cccggtgccg gaaggttaag gggatttgtt agccgcaagg cgaagctttg aaccgaagcc
     2341 ccggtaaacg gcggccgtaa ctataacggt cctaaggtta aagtgaaaaa cgctagcctt
     2401 agtaaaacta aaccatatgc gggaaactcc tcaacagccg ttcgatactc gtttcgcgga
     2461 cgacgcggaa cagtaaaaat tcgacggaca gggggacaat ccgcagggaa ggcccggatc
     2521 gtttgaaatc cgggaaccct cagagacttg atgtttggag ttgacttatg aatatcagtg
     2581 cggaatggat cacaggattt gttgatgggg aaggatgctt tcatatcggc atcaatactc
     2641 atgatgaaat gacggcaggt tttcaggttt taccggaatt caccgttgtt cagcatcaaa
     2701 gagatgtgca aatcctgcat gctctgaaag cgtatttcca gtgcggcgtc gtgaggacca
     2761 atcacggtga ccggatggct tatcgcgtaa gagcaaaaga gcatttgctt gaaaaaatta
     2821 ttccattctt catgaagcac ccgttgaaat caaagaaacg ccaggatttt ttaaagttcc
     2881 gaaaggttgt tttgatgatg gaaaagggtg agcatttgac tgacgagggg attgaaagga
     2941 tccgtgagat agccggtcaa atgaatcgaa gtcaattaag gtaaagtccg tccttcttgg
     3001 aaacaagtag ggtaaaacga gcgaaattcc ttgtcgggta agttccgacc tgcacgaatg
     3061 gcgtaacgat ttgggcgctg tcttgacgag aagctcagcg aatttgataa accggtgaag
     3121 aagccggtta cccgcaacta gacggaaaga ccccgtgcac ctttactata gcttgacaat
     3181 gaatttcggg ctggtttgtg taggataggt gggaggcttt gaagtgggga cgctagtttc
     3241 catggagccg accttgaaat accaccctga ttattctgaa gttctaacct gggcccgtta
     3301 tccgggtcgg ggacattgtc tggtgggtag tttgactggg gcggtcgcct cccaaagagt
     3361 aacggaggcg cgcgaaggtt ccctcaggct gattggaaac cagccgtaga gtgtaaaggc
     3421 ataagggagc ttgactgcga gacaaacaag tcgagcaggt gcgaaagcag gtcttagtga
     3481 tccggtggtt ccgcatggaa gggccatcgc tcaacggata aaaggtacgc cggggataac
     3541 aggcttatca cgcccaagag ttcacatcga cggcgtggtt tggcacctcg atgtcggctc
     3601 atcgcatcct ggggctggat aaggtcccaa gggtttggct gttcgccaat taaagcggta
     3661 cgtgagctgg gttcagaacg tcgtgagaca gttcggtccc tatctgttgt gggcggagga
     3721 tatttgagag gagctgtccc tagtacgaga ggaccgggat ggacgaaccg atggtgttcc
     3781 agttgtcgcg ccagcggcat cgctgggtag ctaagttcgg aagggataac cgctgaaagc
     3841 atctaagcgg gaagcccacc tcaagataag atatccctcc tgacggagac gtcaggacta
     3901 aagacccctc gtagaccacg aggttgatag gtcagatgtg taagcgcagt gatgcgttca
     3961 gcttactgat actaatcggt cgtgcggctt gaccataata attatttata tatggtctcc
     4021 tattcaaaca ggctcaattt tcaacaaagc gatatgcatt gtcatgcatt tattgagata
     4081 aatttcggtg gctatcgcga ggaggtcaca cccgttccca tcccgaacac ggaagttaag
     4141 ctccttcgcg ccgatggtac tgcatgggcg actgtgtggg agagtaggtg ccgccggatt
     4201 taaaacagta aagccccgct gatttttcag cggggctttt tttgtggcgc ggaattcgcg
     4261 tgcgggacag ccgaaaggct tcccctcacg tttgttctgt tcttacccga tcccgaaatc
     4321 ggataaacat catcggccgg gctgctgatc tttttttgcc ctggcctcct cttcatcccg
     4381 taaaaccttg cgcaggattt tgccgacggc cgacttgggg atcgattccc ggaattcaac
     4441 cagcttgggc accttgtaag gcgccagctt ttctttgcag aaggaaataa tttcctccgc
     4501 cgtgcagctt tcgcctgctt tcagaacgac aaacgccttg accgtttctc cccggtattg
     4561 gtctttgacc cccagcgata cggcttcggc gactttggga tgctggaaga ggacttcatc
     4621 aatgtcgcgc gggtaaatgt tgaacccgcc ggcgataatc atatctttct tgcggtccac
     4681 aatgaagaga taaccgtctt catctctgac ggcgatgtct ccggtatgga gccagccgtc
     4741 tttgagttgt ccttccgtct cggcgggatt attccagtac ccctgcatga tcaacggtcc
     4801 cttcatgatg atttcacccg gctctccgtc gggaacatcc gtttctccct tgtccacatc
     4861 caccagccgc acgtcgttat ccgggaaagg aatgccgatg ctgcccactt tcttcaatcc
     4921 cagaaccgga ttggagatgc ccagcgacgt cgattcggaa aggccgtagc cttcactgaa
     4981 ataaatgccc atatccttga cctgttcgat aagttccacg ggcatcggcg ctccaccaga
     5041 attcagcagg ccgaactttt tgttcaaatt catttcggct gctttgggat ggttgatgag
     5101 agcggtgatc agcgtgggaa ccgtcgggaa aaaggtgatc tgatcgaatt tcgcgagaag
     5161 gtccatcatc tcatcgatgt tgaagcgggg aacctggatc tgggtggcgc agttgaacag
     5221 cgcccagttc atgacgacaa tgttgccgta aacatggaaa taaggcaaca cggccatgac
     5281 attgcgtttt tcatgggccg tgagcgtaat cgtagagttt ccccacagcg agcactgaaa
     5341 cgttcccgca acaatattgg cgtgggtgag aacggcgcct ttggggacgc cggttgtgcc
     5401 gccggtaaat tgaatcaggg ccgggtcttc gggattgacc tggactttta cccgtttggg
     5461 attcggatgc ttggcgatga gttccgaaaa gtgacgccag cccggctcca gatcaaggct
     5521 cttggcggaa ctcacgggga atcccttgat atagtcggtg acggcggtga cgatgacgcg
     5581 ggaaatcaga acctgcttgc acagggttcg aatggccggg agagccatat caaaggtgat
     5641 cagagaggtc atgcccgacg tgtcggccat cagcttcagt tcgtcggggg tgtataacgg
     5701 attgagattg acgacaatgc cgcccagcga taaaaccgca aaataagaaa tcagatactg
     5761 agggcaattg ggcagatgca ggccgacgcg atctcccttt ttgattccca gttcggccag
     5821 cgcgttggca aaacttttcg atacaatgct cagctccaga aaagtcatct cactgccgaa
     5881 gaaaatcaag gcggctttgt ccggataagc gccggtgggc agattcagca actcatgagc
     5941 tgccagacgt ggataacgga tggtggttgg tacgttgtaa tcgtagtgac gatgccaagg
     6001 tttctctatc attttccccc tcctcaataa ttcaatttac gaaacagacc acctgctgcc
     6061 gctgagccgg cgtgatgcgg gaaaaacgcg cagggatgaa agaacattcc tcgcgagagc
     6121 tttgagagag aaccgggtaa aacggttttt gcgcatcaga cgactctact tcctctattt
     6181 gacgaaacgc tgctttttgt atgcgcagat taatagtgtg tttcggcgtg gctgtcaatg
     6241 aaatattacc ggctctccag ggaggtttct ccctttacag atgtcattaa aaaagctacc
     6301 taaacccccg ggggggaccg aaaggtcgga attgtcctta ccccccccct attacatcga
     6361 tgaaaggaaa aaatgatgat gaatctttca caattttcac tggaaggaaa agttgccgtc
     6421 gtcaccggag gaagccgggg aatcggccgc gccgccgctt tggcaatggc ggatgccggg
     6481 gcgcacgttg tggtgagcag ccggaaaatc gccgatctcg aaggggttgc cgaggaaata
     6541 tcggccagag gcgtaaaggg aagggccatt gcggcgcatg tcggcaagat ggatgattcc
     6601 agggcgctcg tcgatcaggt cgtgaaggaa ttcggacgcc tcgacattct cgtcaataat
     6661 gccggcacca acccctatta cggaccgatg atggaccagg atgaaaagac ctacgacatc
     6721 accatgaacg tcaacgtgaa aagcatcttc ttcctgagcc agatggcggc aaagatgatg
     6781 aagacgcagg gcggcggctc gatcatcaac acctcttcca tcggcggaat ccgggcgggc
     6841 gagttgggcg tttactgcgc caccaaggcg gccgtcatca tgctcaccca ggtcatggcc
     6901 agggaatggg gacaatacgg cattcgggtt aacgccatcg ccccgggtat catcaaaacc
     6961 cgtctgagtg aagcgctgtg gaaaaatccg gaggtcaacg ccagggcggt ggctcagatc
     7021 cccatgatgc gcctgggcga accggaggaa ctggccggcg ttgtggtgtt cctggcctcc
     7081 agggcgggga gttacgtcac cggcgaaacg attgtgatcg acggcggccg cgttcacggc
     7141 gaaccggcct ggcgggaaaa gaaataggtg tttgggggac gcaacactta attaacgcta
     7201 ttataaaacc tttgaaataa tccagtctcg aattcagtgt tgtgcccccc tcctcttaca
     7261 gcttcagcgc cttttggagc atttccttgt gcgattgaac gaacgggccg tcttcgcgcc
     7321 aggcgtggca ggtgttgatc aggaatttca gaataagctt tgcctgttgg ggatcaaaat
     7381 cgtacggctt tctcgaccgg tgcgcggcaa atccctggac ggccgccggg gccatggcca
     7441 gcagcagctc cagcagatgg gtgcagcctt ttgttccccc gaccagtttt ttgactttgg
     7501 aggtgaagcc tctggtaatg gtcagacccc tgatgggagc gagactgtcg atcgtttccc
     7561 ggcagacgct ccgcggaacc gagatcagat cgacgtcgat gtcctcaatc aaaaaagtgg
     7621 agcagttgac cagcagacgg atgcccatgt ggtgaattac gccggccgga aacgtttcgc
     7681 ccgtaaccgc aaatgtgttc tgatgtcggt catctttcag aaaaccttcc acgatgattc
     7741 tgttttcgtc gcattcgtag gttgatattt caatgttccg cgtgtgcagt tttttccctt
     7801 gcgatttcca ggcagcatcc attttcctcg cccccgttgt atttgaaacc tgtataatgt
     7861 ctcttagtca ttgcgaacga agtgaagcaa tctcatgagt atttgaagag atcgccacgt
     7921 cgcttcgctc ctcgcgatga caaaataaca atgatgcaaa gttctcgatg tatttgtccg
     7981 cgacagacta cagccgcttg agccatgcat aaggcctgcg gcgtttcaga taatatttgt
     8041 cctgttcatg agcgtaggcc tctttttcaa aggcgatatt ccggtaggcg tttcctttcc
     8101 cgaatagccg caccagccat tccactgcat accacagata aaacaagatg aagagcattt
     8161 ccttcatttg cgccgagtga atggcctcat ggttgatggt tttctgcagg aggcctccgc
     8221 ttttcggcgc aaacacgatt ccgaacaggt tgatggccgc cagcttgcgg cccggcatca
     8281 ggtcggtgcg aacaattttc atgattctgc gatcacccga ttgctcaacg ttccgatttt
     8341 ttcgatttca atgcggatat catcgccggg ctggagaaaa agcggcggcc ggcgggtgaa
     8401 gccaacgcct tccggcgttc cggtcagaat cacggtgccc ggatggagcg tcatggaacg
     8461 gctcaggcgg ctgacgattg tccggacgtc aaaaatcatc tccgatgtac gggatttctg
     8521 aacggtcttt ccgttcaaca cggcccggat ggccagatca tccggattgt cgatttcatc
     8581 ctttgtgacg atccacggcc ccagtggaca gaaggtgtca aagctttttc cccgcgccca
     8641 ctggtctttc tgcctttcaa actgccagtc gcgcgcgctg acgtcgttgg cgcaggtgta
     8701 gccgaaaata tagtccggcg cttcctcggg cgtgatgttt tttgccgttt tcccgatgac
     8761 cacggccagc tccgcttcat agtccacctg atcgggcccg gcggcgggga gctcaatggg
     8821 gtggccggga ccgatgacgc tgctggtggc tttgagaaac aggatcggct ccgaaggggc
     8881 ggacagggcg gtttcctcgc cgtgctttcg gtaattgatg cccagtgcca gaatattgac
     8941 gggcgcgacg ggcgccagca gccgttcaat ttgctcctca cggtcgccga cgctgcgcgg
     9001 attccaggga tctccttcaa tggggttggc cacctccggt cggcgcgggt cgtaaacacc
     9061 gtatagaggg ccttttcttt tggaaacaaa tcggacaatt ttcatctcaa aacatctcct
     9121 tttccagaat gctgtcgaat tggaaaaata ccttgcggtc gatcaggatg ccgtcgtgtg
     9181 tgcagtcaaa cccataaaca cggacggcgc aggaaaccgc ccggatgtcc agcgccgtct
     9241 ttcccgccag tttgtcgttc tttccgtaaa aaagataata atgggttgtg gaaggcagtt
     9301 tggtgttcat cgtggcggaa ataaaatcgc tttgcgttcc cagatccagc cagaccggaa
     9361 tgcttttatg cggcagcatt tgggaggcgt cggccagacc gaatccgctc cagggcgtgg
     9421 cgagggatac aaaaagccgg acgaacgtgt tttgtccctc cgatcggcgc cgatccagga
     9481 aggctttcgt ggtcaggccg ccgacgctgt gcgcgacgag ggccattttc cggaaaccga
     9541 atttttcgtg aagctcacgc aattcttcat cgagcagggc cgaggcaaga ttcaggcgga
     9601 ttcccgacgg atagtagaag aaccagggct ggtatttgct gcggtccaga cgcatataaa
     9661 aatatatcca gttgtgcgga ctgccctctg tgccgtgcac gaaaaggaga gggattttcc
     9721 ggggatcgta ggcctgcgtc agatagatat gcgcgccgaa cattttcatg aaggacgatg
     9781 gattccagta gccaatctgc gcgttttcca aagaaaaata ttcatgataa attttcagaa
     9841 catctccgtt gccggttgcc tgccggatga tgggctcccc gattttccgg cgccatcccc
     9901 ggggcagctg caccggctgt tcatcggttg cgatggtccg gatctccacg ccggaaatca
     9961 gctctccttc ccgaaccgca atgtccaggg tctggcttcc cgatccgctc atgccggatg
    10021 cttcatcctc ttcgtacgtt ccattgtgat tgtagtccgt aacggcaaaa agccggtagc
    10081 gcccctcggg caggtacagc atgaaagtat cctgattcaa accggtggcc gtgtattcgg
    10141 cgcattcatt ttttttgtcc gcggggctgc aggccaggat gagcgtggag ttcccccgcc
    10201 atccgggccc ggtgaggcgc cctttgatga ccgcggaatt gtaagcgggc ggtcctttct
    10261 ctcccttcaa ggaaaggaga gagggaacgg cgtcatcctg ggacgggatg ctcaaatacc
    10321 ggcagcccgt caggacggcg gtcatcatga tcagcgccgc ggcaagcctc caccggaaat
    10381 cgtgcaagcg tcttttcatg gcttccccat ctttctttcc agatactcga caacgttgcc
    10441 gagtcttttt tccctggcta gatcgagcgc tgtttttccc tctttgttct tcgccttcag
    10501 atctgcgccc cgctccgcca gcaggataat cgttcttttc gatcctgatt cgacggcgag
    10561 catcaacggg gtatcgcctt gcctgtcggg gcggttcagg tcggctttcc gccggatgag
    10621 ggcggctgcg atgtccggca gatcctctgc ggcggccagg tgcagcgccg tctggccctc
    10681 ctgattggcg gtctggaggt ttccggtttg atccaaaagc agcctggcga ctgaaacgcg
    10741 gccgtttcgg atgcttttca tcaacggatt ttcgcccgct tcatctctgg cggcggtgtc
    10801 cgcgccttcg gcgagcaaaa gcaggacgtt gtcgtggtcg ccctttttcg ctgccagatg
    10861 aagcggagtt ctgcctctgc cgtctttcag gttcacattg gcgtggcgcc ggatcagcat
    10921 cgcggcgatg tcggagcaac ggtagtcgag cgccaggtgc agcgccgttt ccccgaatcc
    10981 gttttgcaaa tcgaggccct ttcccgttgc cagcaagggc ggaatgcagt cccagctctt
    11041 gttgtaaatg gcccacatca acgccgtgta accgtgtcgg tcctgcgcct ccggatccgc
    11101 gccaaacgcc agcagcagtt ccacaatgtc cggatgccgc tcatttgccg caatcatcag
    11161 aggcgtcagc ccgtccgcat cctgccgatt gatttgcgat ttccggagaa gaaagtattc
    11221 ggcaaggccg acgtggccgt tttgaacggc gtacatcaga ccagtccagg cgtaatcgtc
    11281 cgtctggttg acgtcgattt gcttttcctc caccaggcgc atgaccagga cgatgtcccc
    11341 ttttcgggag gcttcgaaaa aagaggtctg tggatcggca agggccgcgg ttaccttctc
    11401 atcttttttt tgcagaggcg gcggcatgca ggcggcaatc gaggcgatga aaaacagcat
    11461 gatcagcgtt acgatggcgt tgcgcatgaa cccaaactcc ccaaattgac ggtgttcatg
    11521 aatggatccg atcaacttct atcatgacgg atgaacgaat tcaaccttcg gctggggcag
    11581 aacaggcctt gtcatggcag ggtgttttcc tgtataagaa cattgcaggt cctgcgcctt
    11641 ggaggtgtgg aagaaggcgc cgctgtaaat ccatgccgga aaggaagctt catgacggac
    11701 attctggatt acatcagcac ctggtcggcg atcaagtggg tcgttctggt tctgatagcg
    11761 ggatttatcg gccagtttgg ccggatgatg gcggaagcca tagcgaacag gatccgcctt
    11821 cgccgcgcga aaaagaatcc gacagcggcc ggccgtcagg aaacggaaga gacaaaaacc
    11881 ctgaatgcag gcgctgtccg ggaaagcggg ctttccggct ccgtgcagga aacgccgggt
    11941 ttgccggccg gagcccctgc gtcggatttt ccttcccggc cttggccgtc ccccatcgcg
    12001 ccggacaaaa aagcgctgaa gacgatggcc aaggcccgca agaaagaagc gaagagagag
    12061 ggaaaggaat aaacgacgtg gcgccagtct tcaggccgtt caaaatgtgc attcaaagcc
    12121 aaccgctacg ggctatccgc atggagaact tttcattatt acacttttgt catcgcgagg
    12181 agcgaagcga cgtggcgatc tcttcaaaca tcaaatgctt atgagattgc ttcacttcgt
    12241 tcgcaatgac taagagacat catgcaaa
//
LOCUS       DAOSRM010000065        12229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5197_length_12229_cov_90.562182, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000065 DAOSRM010000000
VERSION     DAOSRM010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5197_length_12229_cov_90.562182"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..841)
                     /locus_tag="PLA58_07195"
     CDS             complement(77..841)
                     /locus_tag="PLA58_07195"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOR62616.1"
                     /translation="MGSGNIRKFEIRLGQTGLTVLIVGMVVLLCLSFLFGVGVGKNID
                     TYPEKISSLPQRFLALFWRPAKVASGQKTAPGTQAKPEQDNVDLTFHQALTSSKTVPI
                     QPQPEAKKTMEVEAFADGKAALPAQPPSVRQQENAALAKSEAAGQKKDVSEKTAADSK
                     SKGRETASASGSYQVYVASLKDRAKADQIHRIVSRAGYASKVVKVDLKEKGLWYRVIA
                     TGFDSKARAEAAADKLSKKARTKCVVRQAAPEKVKP"
     gene            complement(851..2515)
                     /gene="argS"
                     /locus_tag="PLA58_07200"
     CDS             complement(851..2515)
                     /gene="argS"
                     /locus_tag="PLA58_07200"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOR62617.1"
                     /translation="MKKKLIALIARSVEACIGKGLLPEVDLSRIEIEVPANPQHGDYA
                     SNVAMILASQARQNPRKIAQTILENLEDSEGLLEKTQIAGPGFLNFVLKDDLWRSVLR
                     DILEQKDRFGCLPIGEGKKIQLEFVSANPTGPLHIGHARGAVVGDVLSNLLAATGYAV
                     SREYYINDAGNQMNNLGKSVLYRLRELAGEKIEFPDHCYQGEYIREISGEIFRKEGSR
                     FLGENEDETIERFNEIAGAVILDGIRQDLKDFGVTFDCYFSEKELYRENGVGRLLDDL
                     KKQGFIYSDGETLWFKTTAYGDDKDRVVIRKNGEPTYFAADIAYHADKFSRGFDTLID
                     IWGADHHGYIPRLQAAIQALGRDKDDLKIILVQLVNLLRGGEPVAMSTRSGEFVTLRE
                     VLDEVGKDAARYHFLMRRSDSHLDFDLEVAKKQSNENPVFYVQYAHARICSILRMAQE
                     RGMSLPDFAAIKPSLLVEPEERTLIKMMARYPEMLEGAVKTLEVHRITFYLNELAGLF
                     HGYYNKYKVVTDDAALSAARLALVEAVRQVLVNALKIMGVGAPVKM"
     gene            complement(2594..6352)
                     /locus_tag="PLA58_07205"
     CDS             complement(2594..6352)
                     /locus_tag="PLA58_07205"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOR62618.1"
                     /translation="MNQAAGSNVTPASRVPISASGVFFVGLAYIALGAAGLTLAIPPG
                     YASPVFPAAGLALACLLLFGPRVLPGIWTASFLLNCSHAWLFGSLSPKTAAVAAVIAV
                     GATAQAWVGSALIRRTMGQAWRGLESERHLFRFFLLGALAACLVSASISVGGLSALGV
                     IESSESFFTWWNWYLGDALGVMVFAPLTFCFLNRSDELWRERRRQIVVPMLLILGLVW
                     LAFYGTARWEKQSMDSEIEADGFSIANRIQDRLIAHREVLSSLSHFIEATPDFTFLQF
                     RKFTRITLEDNPDISALSFNDLVLLGERPSYEARMSRLSPLGAFRITERDKNQRLIPA
                     AARPYYVAVRYIVPLDKNRPAVGFDINSEPIRRDAIERARRTKAMAVTSPIHLVQDSK
                     KGVGLLEMLPIEETTEASGARKERLIGFAVAVARVDEMIEIATRGKIPSGLIFQLIDT
                     DAPAGGKLLYHSEAPADGSDAQDRTPAWKTTLSMGDRNWELSIYITKKYLQDHRPWLA
                     WAVGVMGLICSALLQMLLLGMTGQAFVVRQKNKEIQGLADSLENRVEERTLQLSDANQ
                     QLTREIAERKATEEALTKSEEQFRTLVSNIPGITYRCLNDKNWTMLYMSSAVEWVTGY
                     PANDFIENSVRSYESIIHREDTAAVEQAIGEALRLDQPWEIEYRILHRDGSERWVYEK
                     GRGVTGEDGALLYLDGFIFDITQSKQARMEIKRQSGLISSLLDSIPDLIFLKDIHGVY
                     LGCNPPFCEFVGRPREEIIGKTDYDLFDADVAEAFRAYDRKMLELRQSRQNEEWISYP
                     DGRKKLVETLKTPYWGADGTLIGIIGISRDITQRKQAEEDLRRMNEAVERQAVLARRL
                     AEQAQQASQAKSEFLANMSHEIRTPMNAIIGMAEMLQETPLNEEQAKYVRVFKAAGEN
                     LLNIINSILDLSKVEAGQLTLESIPFSLRQVVGDVCDMMAVRVDKKQIRLSCRTDSEN
                     CDSVIGDPTRVQQILTNLIGNAIKFTEQGGVEVNVITKKVSSGSPEDDLCNIHISVKD
                     TGIGIPPEKLDVIFERFEQADSSVTRKYGGTGLGLSISRRLAELMGGRLWVESSVGQG
                     STFHFTLLLRIEAKTQQKEPPEKSEKAAFIGLRPLNILLVDDSDDNRLLVKTYLKGDA
                     YRIDEAVNGEEAVALFQSGPYDLVLMDMQMPVMDGYTATRVIRRWEEEQGRDRTPVIA
                     LTAYALKEDAAKSEAAGCDTHLTKPIKKAVLLEAIRQLVRNSGEYKQA"
     gene            6705..7082
                     /locus_tag="PLA58_07210"
     CDS             6705..7082
                     /locus_tag="PLA58_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62619.1"
                     /translation="MGIEKNTVKSIEKSRFDDDQPRPSEEATLKSAPSASEAASKPEA
                     PPKKVDLKAYQDRMAQLKIELNKTLARIKKADERKDARAKDEAMEENRRISDEMWKMT
                     DELEKNNVKLPSDWWGGIGQETP"
     gene            complement(7107..8228)
                     /locus_tag="PLA58_07215"
     CDS             complement(7107..8228)
                     /locus_tag="PLA58_07215"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HOR62620.1"
                     /translation="MKTARNEKGVVLVVAVMLLAVLVLVGFTAAIMTSTDSKISANYR
                     RSNEAFFNADAGVETVLAYLRTNTVTYPTVDATPSIINGGTCPAGQCVQLSMTVPSGY
                     AFSGTVNLYGYNVANRRFVFRMTGTGSNNATRTIETYIRKNTSVPENADGAVAMYGGG
                     PQVAFKKGGGGGYAVDGHDYPVPANPNCNGSACSTSPTGLPAIPGLFTVMAPTTTGNV
                     SAHLGGVPTQTMGISREAEYNAFAQMIIDSGGTGAPLYQTTLGTRANPAVTVIPNGST
                     LNGSGNGAGIIIVEDGGSLQINGNFEYEGLVILRGSGRVFGSGTGNIYGSLVTVSHLS
                     KLIDLTGSINLFYSSAALANLSNIGALNSVQRVAWRDVQ"
     gene            complement(8331..9041)
                     /locus_tag="PLA58_07220"
     CDS             complement(8331..9041)
                     /locus_tag="PLA58_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOR62621.1"
                     /translation="MSICLRHNGPCAIAASNGRNYHAGFTMIELLIAMAVGLVLLAAV
                     YSVFLVQNKELRNQEQITEMQQNARMAMEMISRDLMMAGFGGYNATAILPRCTGTTTA
                     TNAPCVGITAANANSISFAMDVTDNAGTGGPDGDRDDANENITYDVYTSGGVPALGRK
                     SSTSASRMPVVENVSALSFTYLPATGTTATTDLSLIRRVQISITTRTANVDPGTGNYR
                     YFTLTSTVTPRNLMLSGF"
     gene            complement(9056..9529)
                     /locus_tag="PLA58_07225"
     CDS             complement(9056..9529)
                     /locus_tag="PLA58_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOR62622.1"
                     /translation="MKGIKHNIKKRDRQGFTLLEVLIAMFILTVAILALVSVTVTVIK
                     GNASNKMVTTATTLAKDQLETLKNDSLRDDAGFNSIATTNWAVVSGFPQYERRWTVTT
                     ITGNTACTAAGAPSACCKGAGTGNCPDRKNVTMEVRWSLQGSNHNVTLKSILIKK"
     gene            complement(9540..10007)
                     /locus_tag="PLA58_07230"
     CDS             complement(9540..10007)
                     /locus_tag="PLA58_07230"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOR62623.1"
                     /translation="MRSANNKKWRGGFTMLEIMIVIAIIGIMLGVAAPNFMDYMKSRR
                     LSGAAMQMYVDLMNARQQAVTQNKWVSLRLENSHQYKIFTDSNKNGAIDTGESVIDRD
                     IHPDFADVTFTTSAGTIFAFYPNGTAASKTLTLSGSTGSKSITISTAGRVKIN"
     gene            complement(10183..10953)
                     /locus_tag="PLA58_07235"
     CDS             complement(10183..10953)
                     /locus_tag="PLA58_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOR62624.1"
                     /translation="MAMILLDIFAFIFGAAIGSFLNVCIYRIPAETSIVKPSSRCPSC
                     GHPIRFYDNIPILSFLILRARCRDCGAKISWRYPLIELITGLMALLLFGKFGLSLVFL
                     TFFIFTAVLIVIAFIDLDHQIIPDILSLPGIPVFFLAAVFIVKLPWLEALIGLLIGGG
                     VLLAIAFVYELITKREGMGGGDIKLLAMIGGFLGWKSLIFVLLFSSLAGAVVGIAAMI
                     IHKQDTKYAIPFGPFLSAAAVAWIFWGDAFMRFLIFRN"
     gene            complement(10969..11331)
                     /locus_tag="PLA58_07240"
     CDS             complement(10969..11331)
                     /locus_tag="PLA58_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOR62625.1"
                     /translation="MFFYIDKFALFAIDRSEKLPEKSALKLWSEFMKVYTYSEARQRF
                     AEILNIAREEDVIIQRRGGEVFTIAFKKISRSPFAIPGIKTKATTKDILQAIKDSRER
                     NAEPTNPERLEVEHATEE"
BASE COUNT         2788 a         3394 c         3132 g         2915 t
ORIGIN      
        1 attgcctttc gcaagcctta gatttataca aaacgctctc aggcaagcga gaagggtgtc
       61 tttgggcaac aggccgtcaa ggtttgacct tttccggcgc agcttgacgg acaacgcatt
      121 tcgtcctggc cttttttgag agcttatcgg ccgcggcctc ggccctggcc tttgaatcaa
      181 atcccgtggc gatcacacga taccacaggc ctttctcttt aaggtccact ttcacaactt
      241 tcgacgcata ccccgcccgg gagacaatcc tgtgaatctg atcggccttt gccctgtcct
      301 tcagggacgc cacatacacc tgataggatc cggaggccga agcggtttcc ctgcctttgg
      361 atttgctgtc cgcggcggtt ttttcactga catccttctt ctgccccgcc gcttcgctct
      421 ttgccaacgc ggcattttcc tgttggcgca cagacggggg ctgcgccggc aaagcggctt
      481 tgccgtccgc gaaagcttca acctccatcg tctttttggc ctccggctga ggttgaatcg
      541 gcacggtttt ggaactggtc agggcctgat gaaacgtcag atccacattg tcctgctccg
      601 gcttcgcctg tgtgcccgga gctgttttct gtccggatgc aaccttggcc ggccgccaga
      661 acagcgccag aaagcgctgc ggcagggaag aaattttttc gggataggtg tcgatgtttt
      721 tccccacccc gacgccgaac aggaaggaaa ggcacaacag aacaaccatt ccgacgatca
      781 aaaccgtcaa tccggtctgc cccagccgta tttcaaattt cctgatgttc cccgagccca
      841 tggcttctcc ctacattttt acaggcgcgc ccacgcccat tatcttcagc gcattgacca
      901 gcacctggcg caccgcctcg accagcgcca ggcgcgcggc cgaaagcgcc gcatcgtccg
      961 tgaccacctt gtatttgttg tagtagccgt gaaacaagcc cgccagctcg tttaagtaaa
     1021 aggtgatgcg gtgaacctcg agggttttga ccgcgccttc cagcatttcg ggatagcgcg
     1081 ccatcatttt gatcagcgtt ctttcctccg gttcgacaag cagagaaggc ttgatcgccg
     1141 cgaaatccgg cagggacatt ccccgctcct gcgccatccg gaggatgctg cagattcggg
     1201 cgtgggcata ctggacgtaa aagaccgggt tctcgttgga ctgctttttg gcgacctcca
     1261 ggtcaaaatc cagatgactg tccgaccggc gcatcagaaa atgatagcgc gcggcgtcct
     1321 ttcccacttc gtcgagcact tctcgcaggg tgacgaattc tccggagcgc gtggacatgg
     1381 ccaccggttc gcccccgcgc agcagattga ccagttgcac cagaatgatt ttcagatcat
     1441 ccttatcgcg gcccagcgcc tgaatcgccg cctgaaggcg ggggatgtat ccgtggtgat
     1501 ccgctcccca gatgtcgatc agcgtgtcga acccgcggga aaacttgtcg gcatgatagg
     1561 cgatgtcggc cgcgaagtag gtcggctctc cgtttttccg gatcaccacc cggtctttgt
     1621 cgtcgccgta ggctgtggtt ttaaaccaca gcgtctcccc gtcggaataa ataaagccct
     1681 gctttttgag gtcgtccaga agacgcccga cgccgttttc cctgtagagc tccttttcgc
     1741 tgaaatagca gtcgaaggtc acgccgaaat ctttcaaatc ctgccggatc ccgtccagaa
     1801 tgacggcgcc ggcgatttcg ttgaagcgct cgatcgtttc gtcctcgttt tcgccaagaa
     1861 aacgactgcc ttccttccgg aaaatctccc cggagatttc cctgatatac tctccctggt
     1921 agcagtggtc cggaaattcg attttttccc ccgcaagctc ccgcaggcga tacaggacgg
     1981 atttgcccag attgttcatc tggtttcccg cgtcgttgat gtaatattcc ctggagacgg
     2041 cgtagccggt cgccgccaac aggttggaga gaacatcccc caccaccgcc ccccgtgcat
     2101 ggccgatatg cagaggcccc gtcgggttgg cgctgacaaa ctccagctgg atcttttttc
     2161 cttcaccgat cgggaggcag ccgaaccggt ccttttgctc cagaatatcc cgcagcacgc
     2221 tgcgccataa atcgtctttg agcacaaaat tcaaaaatcc ggggccggcg atttgtgtct
     2281 tttccaggag gccttccgaa tcctccaggt tttccaaaat cgtctgggcg atctttcggg
     2341 ggttttgtct ggcctgagag gccagaatca tggccacgtt ggaggcgtag tcaccgtgtt
     2401 ggggattggc cgggacttcg atttcaatgc gcgacagatc cacctccggc agcaagccct
     2461 tgccgatgca ggcctcaacg gaacgggcga tcagggcaat cagtttcttc ttcatgcaaa
     2521 cgtctcaaaa cctttcttta aaaaatgact gccggctttt atcatttgcc gcgcctcact
     2581 gcaagcgtaa ctgtcaggct tgtttgtact ctccggaatt gcggaccaat tgccggatgg
     2641 cctcaaggag aaccgctttt ttgatgggtt tggtcagatg cgtgtcgcag cccgctgcct
     2701 cgctttttgc ggcgtcttcc ttcagggcat aggcggtcag cgcgatcacc ggcgtgcggt
     2761 ctctgccctg ctcctcttcc caccggcgaa tgacgcgcgt ggccgtatag ccgtccatca
     2821 cgggcatctg catgtccatg agcaccagat catacggacc ggactgaaaa agcgcaactg
     2881 cttcttcgcc gttgactgct tcgtcgattc ggtaggcatc ccccttgaga taggttttga
     2941 ccagaaggcg gttgtcgtcg gaatcatcca ccaggagaat gttcaacggg cgcagtccga
     3001 tgaaagccgc cttttcgctt ttttccggcg gctccttctg ctgtgttttg gcttcaatgc
     3061 gcagcaaaag ggtaaaatga aaggtgcttc cctgaccgac ggaactttcc acccagaggc
     3121 gccctcccat cagctccgcc agccggcggg aaatggacag gccgaggccc gttccgccgt
     3181 attttctcgt cacggaggag tcggcctgct caaaacgctc aaaaatcacg tccagctttt
     3241 ccggcggaat gccgattccc gtatccttta cggagatatg aatgttgcaa agatcatctt
     3301 ccgggctgcc ggaggagact tttttggtga tcacgttgac ctccacgccg ccctgctccg
     3361 taaatttaat ggcgttgccg atcaaattcg tcaggatctg ctgcacgcgg gttggatcgc
     3421 cgatgacgga atcgcagttt tccgaatccg tccggcagga aagccgaatc tgctttttat
     3481 cgacccgcac ggccatcata tcgcagacat cgccgaccac ctgccgcagg ctgaacggaa
     3541 tcgattccag cgtcagttgt ccggcctcga ctttggacag atccagaatc gaattgatga
     3601 tattgaggag gttttccccc gccgccttga agacgcggac gtatttggcc tgttcttcat
     3661 tcaaaggagt ctcctgcagc atttccgcca tgccgataat ggcattcatc ggcgtccgga
     3721 tttcatggct catgttggcc agaaattccg acttcgcctg gctggcctgc tgggcctgtt
     3781 cggccagcct gcgggccaga acagcctgcc gctctaccgc ctcgttcatc cggcgcagat
     3841 cctcctcggc ctgtttccgc tgtgtgatat cgcggctgat gccgataatg ccgattaatg
     3901 tcccgtccgc tccccagtac ggagttttca gcgtttccac cagtttcttg cgcccgtcgg
     3961 gatacgaaat ccattcctca ttctgccggg actggcgtaa ttccagcatt ttccggtcat
     4021 aagcccggaa ggcttctgca acgtcggcat cgaagaggtc gtagtccgtt ttgccgatga
     4081 tttcttccct gggtcttccg acaaactcgc aaaaaggcgg gttacagccg agatagacgc
     4141 cgtggatgtc cttcagaaaa ataagatcgg gaatggaatc cagcagggac gaaatcagac
     4201 cggactgccg tttgatttcc atccgggcct gcttgctctg ggtaatgtcg aaaataaacc
     4261 catccagata cagaagggcc ccgtcctcac cggtgacacc gcggcccttt tcatagaccc
     4321 agcgctcact tccgtcccgg tgtaggatcc ggtattcaat ttcccagggt tgatccagtc
     4381 gaagcgcttc cccgatggct tgttcaaccg cggccgtgtc ttcccggtga atgatgcttt
     4441 catacgagcg aacggaattt tcgataaaat cgtttgccgg ataacctgtc acccattcga
     4501 cggccgaact catgtaaagc atcgtccagt ttttgtcgtt caaacaccgg tacgtgatgc
     4561 cggggatatt cgacacaagg gttctgaact gctcttcgct ttttgtcagc gcctcttccg
     4621 tcgcttttcg ctcggcaatc tcccgggtca gttgctgatt ggcgtctgaa agctgcaggg
     4681 tccgctcttc cacccggttt tccagagagt cggccaaacc ctgaatctct ttattctttt
     4741 gccggactac gaaagcctgc ccggtcattc caagaagcag catctggaga agcgcggaac
     4801 aaatcagccc catcacgccc acagcccagg ccagccaggg acggtgatcc tgcaaatatt
     4861 ttttcgtgat gtaaatcgac agctcccaat tcctgtcgcc catggacagc gtcgttttcc
     4921 atgcgggcgt ccggtcttgc gcatcgctgc cgtccgccgg cgcctccgaa tgataaagca
     4981 gctttcctcc tgcgggcgcg tcggtgtcga tcaactgaaa gatcagtccg gaaggaattt
     5041 taccccgggt cgcgatctcg atcatctcat cgactctggc caccgccacg gcaaagccga
     5101 tcaggcgttc cttccgcgcg ccggatgcct ccgtcgtctc ttcgatcgga agcatttcca
     5161 gaaggccgac gcctttttta ctgtcctgca ccagatgaat gggggacgtc acggccatgg
     5221 cttttgtcct tcgggcgcgt tcgatcgcat cccggcgaat cggttcggaa ttaatatcaa
     5281 atcccaccgc cggccggttt ttgtccaaag gaacgatgta gcgcaccgcc acgtaatacg
     5341 gccttgcggc ggccggaatc agtcgctgat ttttgtcccg ttcggtaatc cggaaagcac
     5401 ccagcggcga gaggcggctc attctggctt cgtaagacgg ccgctcacca aggagaacca
     5461 ggtcgttgaa gctgagcgcg gaaatgtcgg ggttgtcctc aagcgtaatc cgcgtaaatt
     5521 ttcgaaattg aagaaatgtg aagtccggtg tcgcttcgat gaaatgactc agggaggaaa
     5581 gcacttcgcg gtgcgcgatc aggcggtcct gaatgcggtt ggcaatggag aaaccgtccg
     5641 cctcgatctc actgtccatg gactgttttt cccagcgggc ggtgccgtaa aaggccagcc
     5701 acaccaggcc cagaatcagc agcatgggca caacaatctg gcggcgcctt tctctccaga
     5761 gttcatccga ccggttgaga aaacaaaagg tcagcggcgc aaaaaccatc acgcccaggg
     5821 catcgcccag gtaccagttc caccaggtga agaacgattc ggaggattca atgacgccca
     5881 gggcgctcag accgcccacg ctgatcgagg ccgaaaccag gcaggccgcc agagccccca
     5941 acagaaaaaa acgaaataaa tgccgctcgc tttccagacc gcgccaggcc tgacccatcg
     6001 tacgccgaat cagcgcggac ccgacccagg cctgtgcagt cgcgccgacg gcaatcaccg
     6061 ccgcaacggc cgcggtcttc gggctgagcg agccgaagag ccaggcatgg gagcaattga
     6121 gcaaaaaaga agccgtccag atgcccggca gaacgcgcgg gccgaacagg agcaagcacg
     6181 ccagcgccag gcccgcggcg ggaaacaccg ggcttgcgta gcccggcgga atggccagcg
     6241 taaggccggc ggcgcccagt gcgatatagg ccagaccgac aaaaaacacg ccggatgcgg
     6301 aaatggggac cctggatgca ggtgtgacat tgctgccggc tgcctgattc acaaaacgct
     6361 gttcctcttc aaggatgatt tgacggcttt cttgaaaaaa atcatatgga aaatggcttc
     6421 gcgtgtcaat cgaatattcg cagtccgaat attctcaaat tgccttgttc aaaggaattg
     6481 tttgactttg ccggttgtct tttttatgat acccaaaatc gcgaaaagga gaccaaaaaa
     6541 tgaaaacaaa gatgatggtg ttgatgctga tttcattttt tgtttgcccg ataggaagcc
     6601 acgccgccta tatcattcat ctgaaaaccg gcggccggtt tgtaacaccc cagtactggg
     6661 aagataaagg gggcatgatt acctttttcg tcatgggcgg aacgatggga attgaaaaga
     6721 atacggtcaa atccattgaa aaatcgaggt ttgatgatga tcagccgcgt ccttcggaag
     6781 aagccacgct aaaatctgct ccttccgcgt ccgaagctgc ttccaaacct gaagcacctc
     6841 ccaaaaaggt tgatttgaag gcttatcagg atagaatggc ccaactgaag atcgagttga
     6901 acaagacgct ggcccgcatc aaaaaagccg atgaacgcaa ggacgcccgc gcaaaagacg
     6961 aagccatgga ggaaaatcgc cgaatttcgg atgagatgtg gaaaatgacc gacgaactgg
     7021 aaaagaacaa cgtcaagctt ccttcagact ggtggggagg aatcggccag gaaacaccat
     7081 aacgatttca tgaaaaacgg gcttttttac tgcacatccc gccaggccac gcgctgaact
     7141 gaatttagcg cgccgatatt ggaaagattg gcaagagccg cagaactgta aaacagattg
     7201 atgctgcccg ttagatcgat gagtttactc aagtgactaa ccgtcaccaa gctgccataa
     7261 atattccctg tgccggatcc gaaaactctt cctgaccctc tcagaatcac caacccctca
     7321 tactcgaagt tgccgttgat ttggagcgat ccgccatctt caacaatgat aatccccgcg
     7381 ccgtttccgc tcccgttcag cgtggaaccg ttgggaatga ccgttacggc gggattggct
     7441 cttgtgccca gtgtcgtctg gtaaagcgga gctcccgtgc cgccgctgtc gataatcatc
     7501 tgagcaaaag cattatattc cgcttcgcgg ctgatcccca tcgtttgtgt cgggacgccg
     7561 cccagatgag cggaaacatt tcctgtggtc gtcggcgcca ttacggtaaa cagccccgga
     7621 atggcaggca aaccggttgg ggatgtacta caggcagagc cattacagtt tgggttcgcc
     7681 ggaacaggat aatcatgccc gtcaacggca taaccgccgc cgccgccttt tttaaacgcg
     7741 acctgcggac ctccgccgta catggccaca gcgccgtccg cattctcggg aaccgacgta
     7801 tttttccgga tgtaggtttc aattgttctg gtggcattat tggagcccgt gccggtcatg
     7861 cgaaaaacga agcgccggtt ggcaacattg taaccgtaaa ggttaacggt gccggagaaa
     7921 gcataccctg acgggacggt catggaaagc tgaacgcact ggccggcggg acaggtgccg
     7981 ccgttgatga ttgacggtgt cgcgtcaacc gtaggatagg tcacggtgtt cgtgcgcagg
     8041 taagccagaa ccgtttcgac tcccgcgtcg gcattaaaaa acgcctcatt gcttcgtctg
     8101 taattcgcgc tgattttcga atcggttgat gtcataatag ccgccgtaaa gccgaccagc
     8161 accaaaaccg ccagaagcat gaccgcaacc acgaggacca cgcccttttc attgcgcgct
     8221 gttttcatca attgcggaaa cctcataaat cacctcaatt tacgaacgaa atttgaaaca
     8281 ttcaacagga tacatgcgct gttcttgatt ccctctattc aactgatcat tcaaaaaccg
     8341 gaaagcataa gatttctggg tgttacagtg gatgtcagcg taaaatagcg ataattgccc
     8401 gtacctggat ccacgtttgc cgtccgtgtt gtaatgctga tttgaactct gcggataagc
     8461 gaaaggtcag tggtggctgt cgttcctgtc gcaggcagat acgtgaagga gagggccgat
     8521 acgttttcaa ccacgggcat cctgcttgcc gaagtggacg atttgcgtcc cagggccgga
     8581 acaccgccgg atgtatagac gtcataggta atgttttcat tggcatcgtc acgatcaccg
     8641 tcggggccgc cggtgccggc attgtcggtc acatccatcg caaagctgat ggaattggcg
     8701 tttgccgccg taatgccgac gcagggcgcg tttgttgccg tggtcgtccc cgtgcagcgg
     8761 ggcagaatgg cggtcgcatt gtatcctcca aaaccggcca tcatcagatc cctggaaatc
     8821 atttccatcg ccatgcgggc attttgctgc atttccgtaa tctgttcctg atttctgagt
     8881 tccttgttct gaaccagaaa cacggaatag acggccgcca gaagaaccag ccccaccgcc
     8941 atggcaatga gcaattctat cattgtaaac ccagcatggt aattgcgacc gttcgaagcg
     9001 gcaattgcac acgggccatt atgtcgaagg caaatactca tgatcagtta tccacctact
     9061 tctttataag aatgcttttc agcgtcacgt tatgattgct gccctgcaat gaccagcgga
     9121 cttccatagt aacattcttt ctgtcgggac aatttcccgt cccggcgcct ttgcaacagg
     9181 cggaaggcgc tcccgcagcc gtgcaggcgg tatttccagt gatcgtcgtg acagtccacc
     9241 ttctttcata ttgtggaaat ccggacacaa ccgcccagtt ggttgtcgcg atgctgttga
     9301 agccggcatc atccctcaaa ctgtcatttt ttaaagtttc caactggtcc ttggccagtg
     9361 ttgtagccgt tgtgaccatt ttgttggagg cgttcccttt aatcacggtg accgtaacgg
     9421 aaaccagcgc cagaatcgca acggtcagga taaacatggc gatcagcact tcaagaaggg
     9481 taaatccctg acgatccctc ttttttatat tatgttttat tcctttcata tccgccccgt
     9541 caattgatct tcacacgccc cgccgtgctg atcgttatgc tttttgaacc agtcgacccg
     9601 ctaagggtga gggttttgct tgccgccgta ccgttgggat agaaagcaaa aatcgtcccc
     9661 gccgaggttg taaaggttac atctgcgaaa tccggatgga tatcccggtc gatgacgctt
     9721 tctcccgtgt cgatggcgcc gttcttgttg ctgtcggtaa atattttata ttggtggctg
     9781 ttttccaacc tcagcgagac ccacttgttc tgagttacag cctgctgcct ggcattcatc
     9841 aaatccacat acatctgcat ggcggcgccg ctcaaccggc gggatttcat gtagtccatg
     9901 aagttgggag cggcaacgcc caacatgatc ccgataatgg cgatcacgat catgatttcc
     9961 agcatcgtaa atccgccgcg ccattttttg ttgtttgccg atctcatttt ctcacccggt
    10021 tactgaagag caaatcatat gccaacatta tccgacaaag catcaatatc attcatgtag
    10081 ctgtatatta cgttcttaag cgcccgttgt caatggtttc cggtttggag attcgagact
    10141 tcgccggttt tttgcaggca tgggcggcgc ttttcaccgc ttttaattcc tgaagatcaa
    10201 aaacctcatg aaagcgtctc cccagaaaat ccaggcgacg gcagccgccg agaggaaagg
    10261 cccgaaggga atcgcgtatt tcgtgtcctg cttgtggatg atcatggccg caatccccac
    10321 gaccgcgccg gcaagcgaac tgaacagcaa aacaaagatc aacgatttcc agcccagaaa
    10381 gccgccgatc atggccagca gtttgatgtc gccgcctccc atgccttcgc gtttggtgat
    10441 caattcatag acgaaggcaa tggccagcag gaccccgccg ccgatcagca atccgatcag
    10501 ggcctccagc cagggaagtt tcacgataaa gacagccgcc aggaaaaaaa cggggatgcc
    10561 cggcagggac aaaatatcgg gaatgatctg gtgatccaga tcgatgaacg caatgacaat
    10621 caaaactgcg gtgaaaatga aaaaggtaag aaaaacaagg cttaagccga attttccgaa
    10681 aagcagcagc gccatcaggc cggtgatcag ctcaatcagc ggatagcgcc aggagatttt
    10741 ggctccgcag tcgcggcatc tggcgcgcag gatcagaaag ctgaggatgg ggatgttgtc
    10801 ataaaagcgg atcggatgtc cgcaggaggg acagcgcgaa gaaggcttca caatggaggt
    10861 ttccgccggg atgcggtaaa tgcagacatt gagaaagctg ccgatggccg cgccgaagat
    10921 gaaggcaaaa atatcaagca atatcatagc catacctgtg tataacgatc attcttcagt
    10981 ggcatgctca acctccaatc tctccggatt ggttggttca gcatttcgtt ctcttgaatc
    11041 ctttatagcc tgaagaatat ctttagtggt agctttggtt tttataccag gtattgcaaa
    11101 gggagaacgg gatatttttt tgaaggcaat tgtaaatact tcaccgccgc gcctttggat
    11161 aatcacatct tcctctcggg cgatattaag aatctcggca aaacgctgtc ttgcttccga
    11221 ataggtatat actttcataa actcactcca taattttaat gccgattttt ccggcaattt
    11281 ctctgatctg tctatggcaa acaaagcgaa tttgtcaatg taaaagaaca tctacattca
    11341 tgaatactac ttgaaagaga atatgaattt aaaaagaaac cagccgcggg tttccgatag
    11401 gctgaatggc ccggttcgag atcttttatg gtccgtgatg atattcaata atctctccgt
    11461 ccatgaaatg atagatgctg atgtcgccac cgagtcttgg cttcttgaat gcgaaggaga
    11521 aaagccagca tgcgcctttc cgtgggtgac cctcggagta atgatagctg actgaatgaa
    11581 ggtacttgtc agagtagtcc tgctttgcct cggactttag gtattctttg atgcgctcaa
    11641 ccgactgaaa caacgatatc tcgggttcgt gcgatgtggc ttggtcatca gcggggcatg
    11701 accccacgag caggaatgtc gcgacaatcg ctagacatat cgtcttcata tccttaccga
    11761 gattttgcat ctttcatcca gaactcgcag gacgcaattc ctgcaacggc attacgctta
    11821 atagcgccct tgtctttgca gccgcttttt tccttgccgc aggtttcatc ttttctctca
    11881 gaacagagta tggtttgctc attctgtgac accttcctct ctcaacaact ttccgacggt
    11941 tccacgatgt ttcaaattcg tcgatatatt catcttcctg tggatggaat ataaacccta
    12001 tggaataatc tatcaacggt ttttaatgcc aaggaagatc cattgtattc atgctgatac
    12061 gacttcaagc ggaatgccgt ttctgctggc ataggtcgcc gaatatctcc ggagatcatg
    12121 atagctctcg gagatacccc ctctacttaa ggcggggagt ttaattacat acatatatga
    12181 cgaaattttt aagcttgaaa aattagatta cttccactac aaacttacc
//
LOCUS       DAOSRM010000066        12038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5278_length_12038_cov_121.646165, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000066 DAOSRM010000000
VERSION     DAOSRM010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5278_length_12038_cov_121.646165"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1444)
                     /locus_tag="PLA58_07245"
     CDS             complement(2..1444)
                     /locus_tag="PLA58_07245"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOR62626.1"
                     /translation="MSEIKLEKLDDNRWLLPQTGAMRVPGIIYANENIHDLLRHDESA
                     RQVANVAHLPGIVNFSLAMPDVHWGYGFPIGGVAAFDLETGVVSPGGVGYDINCGCRL
                     MATNLTFPDIREKLKELTTALYHRIPSGVGSTGYVKLSGKDQKKVLEEGAAWAVKAGY
                     GSAQDLETTEDGGCLGGADPEQVSARALERGKQQLGTLGSGNHFLEIETVEEIFDETA
                     ARVFGLHKGQIVIMIHTGSRGLGYQICDDYLALMVKHQAQTGISLPDRQLACAYLSSG
                     RGQNYLAAMACGANYAWANRQILMYLTREVLEKTLRASPRELGMRLVYDVCHNIAKIE
                     KFSVAGQEKKLCVHRKGATRAYPAGHEAIPACYRSVGQPVLIPGDMGSGSYVLTGQAK
                     AMDETFGSACHGAGRVMSRSQAIRSSRGRSISRELADRGVLVMAGSKGTLGEEMPEAY
                     KNVHDVVEVVHDAGIAKKAARLKSLACIKG"
     gene            complement(1437..1868)
                     /locus_tag="PLA58_07250"
     CDS             complement(1437..1868)
                     /locus_tag="PLA58_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOR62627.1"
                     /translation="MKKYELFDHTADLGLEIYGRTKRELFANAALALFDVMIQDIEPP
                     TKRGGKERVKTRTVLGADVGDLLVNFLRELLYWFNGLGWVLNRGSILVCGNKKLVVQC
                     TVEPYHPERHLIKTEIKAVTYHQLAVEKTKTGWKARVIFDV"
     gene            complement(1984..4494)
                     /gene="gyrA"
                     /locus_tag="PLA58_07255"
     CDS             complement(1984..4494)
                     /gene="gyrA"
                     /locus_tag="PLA58_07255"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOR62628.1"
                     /translation="MERDIHRRQTLVNIEDEMKKSYLDYSMSVIIGRAIPDVRDGLKP
                     VHRRILYAMSEMGNRHNKPYKKSARIVGDIMGKYHPHGDAAIYDTLVRMAQDFSMRYT
                     LVDGQGNFGSIDGDPPAAMRYTESRLARISDEALADLEKDTVDFVPNYDESLSEPSIL
                     ATRLPLLLLNGTSGIAVGMATNIPPHNLTEIVNGTIALIQNPKMIVEELMKYIPGPDF
                     PTAGFINGREGILAAYKTGRGIIRIRARALIEKNARNDRETIVITELPYQVNKATLIE
                     KISELVRDKKISGISDLRDESDREGIRVVIELKRDESSAIILNQLYKFTQMEVSFGII
                     MLAISEGRPKVFNLKEVLEKFIAFRQEVVVRRTQFDLARARERAHILEGYIIALNNID
                     EVIKVIKAAKSPNDASVALCAAFALSEIQAKAILDMRLQRLTGLERDKIDAEYADVTA
                     LIAKLAGILDDPQKVLKVIVDELQEIREKYGDERRTEIVAGTEDIDIEDMIVEEDMVV
                     TISHAGYIKRNAVSLYKSQHRGGRGKVGMGTKEEDFVEKIFIASTHHYLLVFTSAGKV
                     HWLKVYQIPQAGRAAKGKAMINLVSLAPGERVSATLPVKEFVSDKFVVMVTRKGIIKK
                     TDLAAYSNPRTGGIIAISIDDDDELVDVQLTNGDQDIFLASRLGMAIRFREDDVRDMG
                     RTARGVRGINLDEGDDVIGVEIPAQNTFMLTVSENGYGKCTPIDEYRVQARGGKGTIN
                     LKTVPKIGNVSGVLQVQGEENVMLISSTGNVIRMKVQEIPVNHRVTQGVKLIDLAPEE
                     KLVGVARTTSESEDKDDTNGADEANGNGEENGPPADES"
     gene            complement(4510..6918)
                     /gene="gyrB"
                     /locus_tag="PLA58_07260"
     CDS             complement(4510..6918)
                     /gene="gyrB"
                     /locus_tag="PLA58_07260"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOR62629.1"
                     /translation="MVETYDADSIKVLEGLEAVRKRPAMYIGSTSKEGLHHLVYEVVD
                     NSIDEAAAGFCDSISVKIRVDNSIVVEDNGRGIPVDLHKTEGVSAAEVAMTKLHAGGK
                     FNNDTYKVSGGLHGVGVSVVNALSRACELEVRRDGNVYRQTYKRGIPDGPLQVVGKSR
                     GRGTTVSFLPDDEIFEETEFSFDVIANRLRELAFLNSGVKITLMDERSEKKAEFFYKG
                     GIVSFVEYINRNKKVLHKEPIYVTGAREDCSVEVALQYNDSYLENIFSFANSINTVEG
                     GMHMVGFRAALTRVFNNYATANKIIKDGKESLRGEDLREGLACVISVKVRNPQFEGQT
                     KTKLGNSDVRGLVEGIVYEKIGSYLEENPSVARQILNKCMDAARAREAARRARELTRR
                     KSALEVGSLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAILPLRGKVLN
                     VEKARFDKMLQNEEIKVMVTALGAGIGADDYDVSKLRYHKVIIMTDADVDGSHIRTLL
                     LTFFFRQMRELIERGYLFIAQPPLYKVVDKKKEFYIQNEEQMKNYILENGVNKVNLFM
                     GDGSPFPVSGNKLLTIIKKINRIKDLLDRFEKDHRDRNVVRIAAGDPTLTDETFQNES
                     DLLKIAGRTVVALGSDVVTDYRIDEDPDHGGYKMLFDIRHNGTIHTTCIDREIFRTPQ
                     FGEIRNLMGQVSILGEPPYSDQPEDAAQPVTLPTAKDLVDHIINLGKKGLTIQRYKGL
                     GEMNPEQLWETTMNPEKRTLLQVGVEDAVLADEIFTTLMGDEVEPRREFIYQNAMHVS
                     NLDI"
     gene            complement(6967..8079)
                     /gene="dnaN"
                     /locus_tag="PLA58_07265"
     CDS             complement(6967..8079)
                     /gene="dnaN"
                     /locus_tag="PLA58_07265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOR62630.1"
                     /translation="MEFKVNRNIFLDGIQKTLGIVEKKTTIPILNNVLLRAESNKIKI
                     IATDREISLISDYEADIAERGEVTLSAKKLFEMVREIQGDVVHFTKSANNIVKVTCQR
                     AVYKIPGLPADDFPSVADSQQVEFFSIPGSVLKDLISKTSFAMAFDESRKHLNGVLLE
                     TASDGKNHLWRMVATDGHRLAVAKAITADPCPELAKGIIIPRKGLLEVKKLTDVHENV
                     SVGVHKNMFVVKTENTILKINLVDAEYPDYKRVIPTEKGTSVTLDKELFLHALRRMNV
                     VSSERYSGVIISFTADKMTLNSTNLDVGEATEEIDISYAGEDMDVGFNVNYVIDAVSA
                     ISVDNVVMEVGVGLKPTLIKPAEGDNYLCIVMPLKI"
     gene            complement(8231..9583)
                     /gene="dnaA"
                     /locus_tag="PLA58_07270"
     CDS             complement(8231..9583)
                     /gene="dnaA"
                     /locus_tag="PLA58_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOR62631.1"
                     /translation="MESVWDKATKIIQEKVSKQNFDTWIKPIKIVSMEDKRVQLSVPN
                     KFFKDWLMDNYLSMIKNSLHSVVGISVDIDFMLSKDKEKKPEVAVLPFEQGKNPTAPS
                     PGRNINISFLNSHYTFDRFVVGPSNQFAHAASIAVGKQPAKNYNPLFIYGGSGLGKTH
                     LLNAIGLMTIASHPDLNVMYVSAESFMNEMINSIRYDRMPKFREKYRNIGCLLIDDIQ
                     FLAGKDRTQEEFFHTFNTLHDSGKQIVVTSDKFPKDIPNLEGRLRSRFEWGLIADIQP
                     PEIETKIAIIEKKMHESKMELSPTVTHYIASHVESNIRELEGFLIRISAYSSLTNREI
                     DLDLVKEVLKTLVKHNNKEEVSVEEIIKTVAGKMNVKISDIKAHNKNKNLVLARQISM
                     YLARKLTNLSYPDIGQKIGGRDHSTVIYANNKILKTVESDAAFKKTIQDIEDNIVHKT
                     "
     gene            complement(9635..10492)
                     /locus_tag="PLA58_07275"
     CDS             complement(9635..10492)
                     /locus_tag="PLA58_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOR62632.1"
                     /translation="MAQKKDALGKGLGAIFPDLLETDAGRKAPVNTCGIEELRPNRWQ
                     PRKNFNDEDQKKLVASVRQSGIIQPIIVRRADSGYEIIAGERRWRAAQAAGLKDVPIV
                     IRKASDVEAAELSLIENLLREELGPLEEADAYVTLMETFKLSQESISARVGKDRSTIA
                     NIVRLLKLPDKVKTALTEKKITSGHARCLLALPSPGEQVAALDMILKRELNVRETEKL
                     INRLKQKPAEKKAVVKDRYMADLEKQLTAKCMARVQLKGSSKKGAIEISYRTMDELNR
                     LARYLLDEL"
     gene            complement(10493..11260)
                     /locus_tag="PLA58_07280"
     CDS             complement(10493..11260)
                     /locus_tag="PLA58_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR62633.1"
                     /translation="MSKIICIANQKGGVGKTTTAVNLSASLAAANRKTLLIDADAQGN
                     STSGLGIERMRYEKANFYHAMIGHAPLESVIVQTALPNLDIIASNQDLIGMEIEFVYL
                     ENRESRLRRLLKNLDRSYEFIIIDCPPSLGFMTVNALVAADTLIVPLQCEYYALEGLG
                     HLLNTVQLVKANLNPQLALGGILLTMFDPRNTLSHRVTEDVRKHLGGKVFQTVIPRNV
                     RLSESPSHGLPIILYDIKSRGAIAYMELAQEILRGER"
     gene            complement(11351..11911)
                     /gene="rsmG"
                     /locus_tag="PLA58_07285"
     CDS             complement(11351..11911)
                     /gene="rsmG"
                     /locus_tag="PLA58_07285"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOR62634.1"
                     /translation="MQWNAKTNLISKKTAEEIATRHFLDSLTAARYIRFENARMVDIG
                     TGAGFPGLPLKIALPSIQLCLLESNRKKVSFLKHIQRLLNLDGVFTLQDRTENIVREG
                     QWREKFDVVISRASLKLPDLMPLGQYFLAPGGLLITLKGPDVSPEVDAAFSGENHGNI
                     FQLYQEDINLPLLGPPRKIIILEKAK"
BASE COUNT         2518 a         3294 c         3064 g         3162 t
ORIGIN      
        1 gtcatccctt gatacaggcg agggatttga gccttgccgc ttttttggcg atgccggcgt
       61 catgaaccac ttccacgacg tcatggacat tcttgtaggc ttcgggcatt tcctcgccga
      121 gcgtcccttt gcttccggcc atcaccagaa ctccccggtc agccagctca cggctgatgg
      181 accggccccg gctggagcgg atcgcctgcg accggctcat gacacggccc gcgccgtggc
      241 aggcgctgcc gaacgtttcg tccattgctt tggcctggcc ggtaagcaca taggatccgg
      301 accccatgtc ccccggaatc agcaccggct ggccgacgct ccggtagcaa gcgggaatgg
      361 cttcatggcc cgccggatag gcccgcgtcg ctcccttgcg gtgcacgcag agttttttct
      421 cctgtccggc cacggaaaat ttctcgattt tggcgatatt gtggcagacg tcatacacca
      481 gccgcatccc cagctcgcgg ggggaagcgc gcagggtctt ttccagaacc tcccgcgtca
      541 gatacatcag gatctggcgg ttggcccagg cgtagttggc cccgcaggcc atggcggcca
      601 gatagttttg cccgcgcccg gaggacagat aggcacaggc cagctgacgg tcgggaagcg
      661 agatgccggt ttgggcctgg tgtttgacca tcagcgccag gtagtcgtcg caaatctgat
      721 accccaggcc ccgagacccg gtgtgaatca tgatgacgat ctggcctttg tgcaaaccaa
      781 aaacccgcgc cgcggtttca tcaaagattt cttcaacggt ttcgatttcc agaaaatgat
      841 tgccggagcc gagggtgccc agctgctgct ttccccgctc cagggcgcgc gccgaaacct
      901 gctccggatc tgcgccgcca aggcatcctc cgtcttccgt ggtttccaaa tcctgcgcgg
      961 agccgtatcc cgctttcacc gcccaggccg ccccttcttc cagcactttt ttctgatcct
     1021 tgccggagag tttgacgtag ccggtggaac cgacgccgga cggaatgcga tgatacaggg
     1081 ccgtcgtcaa ttcctttagt ttttcccgga tgtcgggaaa cgtcaggttg gtcgccatca
     1141 gacggcagcc gcagttgatg tcatagccca cgccgccggg cgacaccacg cccgtttcca
     1201 ggtcgaaagc cgccacgccg ccgatgggaa agccgtaccc ccagtgaaca tccggcatgg
     1261 ccagcgaaaa attgacaatg cccggcagat gagcgacgtt ggccacctgc cgggcgcttt
     1321 catcgtgcct cagcaaatca tgaatgtttt cattggcata gatgatgccc ggcacacgca
     1381 tggcgcctgt ctgcggcaaa agccagcggt tgtcatcgag tttttcaagt ttgatttcag
     1441 acatcaaata tcaccctcgc cttccagccg gttttcgttt tttccacggc cagttgatga
     1501 tacgtgacgg ctttgatctc cgttttgatc aagtgccgtt cgggatgata cggctcaacc
     1561 gtacattgca cgaccagctt tttgttgccg cataccagga tcgaaccgcg attcagcacc
     1621 cagcccagcc cattgaacca atacagaagc tcccgcagaa agttcaccag caaatccccg
     1681 acatccgcgc ccagcacggt tcttgttttg acccgctctt ttccaccgcg ttttgtcgga
     1741 ggctcgatgt cctgaatcat cacgtcaaac agggccaggg cggcgttggc gaaaagctcc
     1801 cttttcgtcc gcccgtaaat ttccaggccc agatcggcgg tatgatcaaa aagttcatat
     1861 tttttcatgg gggaattata gcgttgtgcg gaaggataaa caagaaccaa tggaaagcaa
     1921 acccaaaact attgacgtac cccggcgagc ggggaacgca acgccgttcc ccgctcgcgg
     1981 attttacgac tcgtccgcag gcggaccgtt ctcttccccg ttgccgttcg cctcatcggc
     2041 gccgttggtg tcgtccttgt cttccgattc cgatgtcgtg cgggccacgc cgacgagttt
     2101 ttcttccggc gccagatcaa tcagtttgac accctgggtc acacggtggt tcaccggaat
     2161 ttcctgcact ttcatccgga tgacgttgcc cgtgctggag atgagcatga cgttctcctc
     2221 gccctgcacc tgcaggacgc cggaaacatt cccgatcttc ggcacggtct tcaggttgat
     2281 cgttcccttg ccgccccgcg cctgaacgcg gtattcgtca atcggcgtgc atttgccgta
     2341 gccgttctcc gaaaccgtca gcataaaggt gttttgcgcg ggaatctcca cgccgatgac
     2401 gtcatcgccc tcatcgaggt tgatgccgcg gacgccgcgg gccgtgcggc ccatgtcgcg
     2461 cacgtcgtct tcccggaagc ggatcgccat gcccagccgc gaggccagga aaatatcctg
     2521 atcgccgttg gtcaactgca catccaccag ttcgtcatcg tcatcgatgg aaatggcgat
     2581 aatgccgccg gtgcgcggat tggaataagc cgccagatcg gtttttttga tgatgccttt
     2641 gcgcgtgacc atcacgacga acttgtccga aacaaattcc ttgaccggca gcgtcgcgct
     2701 gacgcgctct cccggcgcca ggctgaccag attgatcatg gccttgccct tggccgcccg
     2761 cccggcctgc gggatctgat acactttcag ccagtgcact tttccggcgc tggtgaagac
     2821 caggaggtaa tgatgggtgg aagcaatgaa tattttttcg acaaaatcct cctccttggt
     2881 gcccatgccg accttgcccc gcccgccgcg gtgctggctc ttgtacagac tgacggcgtt
     2941 gcgcttgatg tagccggcat gcgagatggt gaccaccatg tcttcttcca cgatcatgtc
     3001 ttcaatgtcg atgtcttccg tgccggcgac gatttccgtg cggcgctcat cgccgtattt
     3061 ttcccggatc tcttggagtt catcgacaat cactttcagc accttttgcg gatcgtccag
     3121 aatgcccgcc agtttggcga tcagcgccgt aacgtccgcg tattccgcgt cgatcttgtc
     3181 ccgctccagg ccggtcagcc gctgcagacg catgtccaga atggctttgg cctgaatctc
     3241 ggacaaagca aacgccgcgc acaacgccac ggacgcatcg ttggggcttt tggccgcctt
     3301 gatgaccttg atgacttcat cgatgttgtt cagcgcgatg atatagcctt ctaaaatatg
     3361 cgcccgctcc cgggcgcgcg ccagatcgaa ctgtgtgcgc cggaccacca cttcctgacg
     3421 gaaggcgatg aatttctcaa gcacctcttt gaggttgaaa actttcggcc gtccttcgga
     3481 aatcgccagc atgatgatgc caaaggagac ttccatctgc gtaaatttgt aaagctggtt
     3541 caaaatgatc gcggagcttt catcgcgctt gagttcaatg accacgcgga tgccctcccg
     3601 gtccgattcg tcgcgcagat cggagatgcc ggagattttc ttgtcgcgca ccagctccga
     3661 aattttttca atcagcgtcg ccttgttgac ctgatacggc aattcggtaa tgacgatggt
     3721 ctcccggtcg ttgcgggcgt tcttttcgat gagcgcgcgc gcccggatgc ggataatgcc
     3781 gcgcccggtt ttataggcgg caagaattcc ctcccggccg ttgatgaagc cggccgtggg
     3841 gaaatccggg ccgggaatgt atttcatcag ctcttccact atcattttgg gattctgaat
     3901 cagggcgatc gtcccgttga cgatttccgt cagattgtga ggcggaatat tggtggccat
     3961 gccgacggcg atgcccgacg ttccgttcag cagcagcagg ggaaggcgcg tcgccagaat
     4021 cgacggctcc gaaagcgatt cgtcgtaatt cggcacaaaa tcgaccgtgt ctttctccag
     4081 atcggccagt gcttcatccg aaatgcgagc caggcggctt tccgtgtagc gcatggccgc
     4141 aggcggatcg ccgtcgatcg atccgaagtt gccctgtccg tccaccagcg tgtagcgcat
     4201 ggagaaatcc tgcgccatgc ggaccagggt gtcatagatg gccgcgtcgc cgtggggatg
     4261 atatttaccc atgatgtcgc cgacgatgcg ggcggatttt ttgtagggct tgttgtggcg
     4321 gttgcccatt tccgacatcg catacaggat gcggcgatgc acgggtttga ggccgtcgcg
     4381 cacgtcggga atcgcccgac cgataatgac gctcatggag taatccaagt aggatttctt
     4441 catttcgtct tctatattca ccagggtttg cctgcggtgg atgtctcgtt ccattttatt
     4501 tcttccttct taaatatcca agttggatac atgcatggcg ttttggtaaa tgaactcgcg
     4561 ccgcggctcc acctcgtcgc ccatcagcgt ggtgaaaatt tcatcggcga gcaccgcgtc
     4621 ttcgacgccc acctgcagga gggttcgctt ctcggggttc atggtggttt cccagagctg
     4681 ttccggattc atttcaccca gacctttgta acgctggatg gtcaggcctt ttttgcccag
     4741 gttgatgatg tggtccacca gatccttggc ggtcgggaga gtgaccggct gggcggcatc
     4801 ttccggttga tcactgtagg gcggttcccc cagaatggaa acctgcccca tcagattgcg
     4861 gatttcgccg aactgcggcg tacggaagat ttcccggtcg atgcaggtgg tatgaatcgt
     4921 tccgttatgt cgaatgtcga aaagcatctt gtagccgccg tgatcggggt cctcatcgat
     4981 gcggtagtcc gttaccacgt cgctgcccag ggcgacaacc gttctgcccg cgattttcag
     5041 cagatcgctt tcgttttgaa acgtctcatc cgtcagcgtg ggatctccgg ccgcaatccg
     5101 gacgacattc cggtctcgat gatctttttc aaaacggtcc aggaggtctt tgatgcgatt
     5161 aattttttta atgatggtca gtaatttgtt acccgaaacg ggaaaagggc ttccgtcccc
     5221 cataaataaa ttcaccttgt tcacgccgtt ttccagaatg taattcttca tttgttcttc
     5281 gttctggatg taaaactctt tctttttgtc caccaccttg tacagcggcg gctgcgcgat
     5341 aaacaaatac cctctctcaa tcaattcgcg catctggcgg aaaaagaagg ttaaaagcag
     5401 cgtgcggatg tgcgatccgt ccacgtcggc atccgtcatg atgatgactt tgtgataccg
     5461 aagtttgctg acgtcataat cgtcggcgcc gatgcccgcc ccgagggccg tcaccatcac
     5521 cttgatttct tcgttttgca gcatcttgtc gaagcgcgct ttttccacat tgagcacttt
     5581 cccgcgcagg ggcaggatcg cctggttttt ccggtcgcgt ccctgtttgg ccgagccgcc
     5641 ggccgagtct ccctccacca ggtaaagctc gcttagggcg gggtcctttt cctggcaatc
     5701 ggccagtttt ccgggaagcg agccgacttc cagcgcgctc ttgcggcgcg tcagttccct
     5761 ggctcttcgg gccgcttcgc gcgcgcgcgc ggcgtccatg catttattga ggatctgtct
     5821 ggcgacggac gggttttcct ccagatagga tccgattttc tcgtaaacga ttccctccac
     5881 gagaccgcga acatcggaat ttcccagttt ggttttggtt tgcccttcaa actgggggtt
     5941 gcggaccttt acgctgatga cgcaggccag cccttcgcgc aagtcttcac cacgcaggga
     6001 ttccttgccg tccttgatga ttttgttggc ggtggcgtaa ttattgaaca cacgggtcag
     6061 cgccgcgcgg aagccgacca tgtgcatgcc gccttccacg gtattgatgc tgttggcaaa
     6121 ggaaaagatg ttttccagat agctgtcgtt gtattgcagg gcgacttcca ccgagcagtc
     6181 ctcccgggcg ccggtcacat agatcggctc tttgtggagc actttcttgt tgcggttgat
     6241 gtattccaca aaagaaacaa tgccgccttt gtagaaaaat tccgcctttt tttccgagcg
     6301 ttcatccatc agggtgattt tgacgccgga gttgagaaac gccagttccc gcaggcggtt
     6361 ggcaatcaca tcaaagctga attcggtttc ttcaaaaatt tcatcatccg gcaaaaaaga
     6421 gacggtggtg ccgcgtcccc tgcttttgcc gacaacctgc aggggcccgt ccggaatgcc
     6481 ccgcttgtag gtctgccggt aaacattccc atcgcgtcgc acttccagtt cacaggcgcg
     6541 ggaaagcgca ttaaccaccg atacgccgac gccgtgcaat ccgccggata ccttgtaggt
     6601 gtcgttgtta aattttcctc cggcgtgcag tttggtcatg gcgacttccg cagccgacac
     6661 gccttccgtt ttatgaagat ccaccggaat gccccggccg ttgtcttcca cgacaatgct
     6721 gttatcaacc ctgattttca cggaaatgga atcacagaac cccgccgccg cttcgtcaat
     6781 gctgttgtca acaacttcgt aaaccagatg atgaagacct tctttgcttg tagaaccaat
     6841 atacatggcc ggacgcttgc gaaccgcttc caggccttct aaaactttaa tactgtcggc
     6901 atcgtacgtt tctaccataa ctgctttttt actccttcaa cctcttcctt gttccataaa
     6961 acaaatttat atttttaacg gcatgacaat acataaataa ttatctccct cggccggttt
     7021 aatcagcgtc ggtttcaaac caacccccac ttccatcaca acattatcaa ccgatatggc
     7081 cgatacggca tcgatgacat aattaacatt gaatccgaca tccatatctt caccggcata
     7141 tgaaatgtca atttcttccg tggcctcgcc tacatccagg ttggtggaat tcaacgtcat
     7201 tttatccgcg gtaaacgata tgatcacgcc gctgtaacgc tcggaagaca ccacattcat
     7261 tctccgcagc gcatgcagaa acaactcctt atccagcgtc acactggtac ccttttccgt
     7321 cggaatcacc cgtttgtaat caggatattc cgcatccacc agattgatct ttaagatcgt
     7381 attttccgtt tttaccacaa acatgttttt atggacgccg acactgacgt tttcatgaac
     7441 atccgtcagt tttttcactt ccagaagtcc tttgcgggga atgataattc ccttggcgag
     7501 ttccggacac ggatcggccg ttatggcttt ggccactgcc agtcgatgtc cgtccgttgc
     7561 caccatccgc cagagatgat tctttccgtc ggaggcggtc tccagcaata cgccgttcag
     7621 gtgtttcctg ctttcatcga aggccattgc aaaagaggtt ttactgatca aatctttcaa
     7681 gactgatccg ggaatactga aaaattccac ctgctgactg tcggcaacac tgggaaaatc
     7741 gtccgcgggg agccccggaa ttttataaac ggccctctga cacgttactt ttacaatatt
     7801 gttggcgctt ttggtaaaat ggacaacatc tccctgaatc tcgcgaacca tttcaaagag
     7861 ctttttggcc gacagggtaa cttcgcctct ttccgcgata tccgcttcat aatcggatat
     7921 cagactgatc tcgcggtctg tggcgataat tttgatcttg ttggattccg cccttaaaag
     7981 aacattattt aaaataggga tcgttgtttt tttctcgaca atgcctaacg ttttctgaat
     8041 cccgtccaga aaaatatttc tgttcacttt gaattccatc gtcgcctccc attagtgttt
     8101 ttattttttt cttaatatac ataatcagta ttagtaataa cgcctgtgga tatgtggatt
     8161 gccttttatt cagctcttgt ttcaatcctt tcggtcttct tctcctgttg aagaggtgtg
     8221 cagactccga ttaagttttg tggacaatat tatcctcgat gtcctgaatg gtttttttga
     8281 aggcggcgtc cgactcaacg gtttttagaa ttttattatt ggcataaatc acagtggaat
     8341 gatcacggcc gcctattttt tgtccgatgt cggggtaaga aagattagtc agtttccggg
     8401 caaggtacat ggagatctgc cgggcgagga ccagattctt gtttttattg tgtgctttga
     8461 tatccgaaat tttgacattc atcttcccgg cgacggtttt aataatttcc tccacactca
     8521 cttcttcttt attgttgtgt ttaacgagtg ttttcaaaac ttccttcacc aggtccagat
     8581 cgatttcccg gttggtgagg gaggaatacg cggatatccg gattaaaaat ccttccagtt
     8641 cgcggatgtt ggattccaca tgcgaggcaa tgtaatgcgt caccgttgga gacaactcca
     8701 tcttgctctc gtgcattttc ttttcaatga ttgcaatttt tgtttcgatt tcggggggct
     8761 gaatatccgc gatcagaccc cattcaaagc gcgatcgcag ccggccttcc agatttggaa
     8821 tatctttcgg aaatttatcc gatgtcacaa caatctgctt tccggagtca tgtagcgtgt
     8881 tgaaggtatg aaaaaattct tcctgcgttc tgtccttgcc ggcgagaaac tgaatgtcgt
     8941 caatcagcag gcatccgata ttccggtatt tttcccggaa tttcggcatc cggtcgtagc
     9001 ggatggaatt aatcatttca ttcatgaagg attccgccga cacatacatg acattcaaat
     9061 ccggatgaga ggcaatggtc atcaacccga tggcattgag taaatgggtt ttccccaacc
     9121 ccgagcctcc gtaaatgaaa agaggattat aatttttcgc cggctgcttg ccgacggcaa
     9181 tggaggcggc atgagcaaac tggttggagg ggccgaccac aaaccggtca aaagtataat
     9241 ggctgttgag gaaagaaata tttatatttc tacccggcga gggggctgtt ggatttttac
     9301 cctgttcaaa aggcaatacg gccacttcgg gctttttttc cttatcctta ctgagcataa
     9361 aatcgatatc cacgctgatt ccgactacgc tgtgcagtga gtttttaatc atggacaaat
     9421 agttgtccat cagccagtcc ttgaaaaatt tattgggcac ggacaactgg acgcgcttgt
     9481 cttccatgga gacgattttg atgggtttta tccaggtgtc aaagttctgt tttgacactt
     9541 tttcctgaat gattttcgta gctttatccc agacagattc catgatcaac ctttttcaaa
     9601 cagccttatc aacaggtgtt gatacatttt tttcttacaa ttcatctaaa agatagcgcg
     9661 ccagacgatt cagttcatcc atcgtcctgt acgaaatttc aatagctcct tttttggatg
     9721 accctttcaa ctgtacacgg gccatacatt ttgccgtcaa ttgtttttcc agatccgcca
     9781 tgtagcggtc tttaacgaca gccttttttt ccgcgggttt ctgctttaac cggttgatca
     9841 gcttctcggt ttcccggacg ttgagttctc tttttaagat catatccagt gcggcgacct
     9901 gctcgcccgg cgacggcagc gccagaaggc agcgggcatg tcccgacgta atcttttttt
     9961 ccgtcagggc ggttttcact ttgtccggca gctttaaaag acggacgatg ttggcaatcg
    10021 tggagcggtc tttgccgacc cgggcggaga tggattcctg agaaagttta aaggtttcca
    10081 tcagtgtgac ataggcgtcc gcctcttcca gggggccgag ctcttcgcgc aggagatttt
    10141 caatcagcga caattcagcg gcttccacat cggaggcctt acggatcaca atcggcacgt
    10201 ctttaaggcc cgccgcctgt gccgcgcgcc agcggcgctc cccggcaatg atttcatagc
    10261 cggaatcggc ccggcgcacg atgatcggtt gaatgatgcc gctttgccgg acggacgcca
    10321 cgagtttttt ctggtcttca tcgttgaaat ttttccgggg ctgccagcgg ttcggccgca
    10381 attcctcaat accgcaggtg ttgacgggcg ctttcctgcc ggcgtctgtt tcaagcaaat
    10441 ccggaaaaat agcgccgagg cctttgccca gagcgtcttt tttttgcgcc atttatcttt
    10501 ctccccgaag aatttcctgc gccagctcca tgtaggcgat cgcgccgcgc gatttgatgt
    10561 cgtataaaat aatgggcaat ccgtggctgg ggctttccga aagcctcacg ttgcgcggaa
    10621 tgaccgtttg gaaaactttt ccgcccagat gtttccggac gtcttccgtt acgcgatgcg
    10681 aaagtgtatt gcgcggatca aacatggtca gcaggatgcc gcccagagcc aactgcggat
    10741 tcagattggc tttgaccagt tgcaccgtgt tgagcaggtg tcccaggcct tccagtgcgt
    10801 aatactcgca ctgcagggga acaatcagcg tgtccgcggc gacgagcgca ttgacggtca
    10861 tgaaccccag cgacggcggg cagtcgatga taataaattc ataagaccga tccagatttt
    10921 ttaaaagccg gcgaagccgg ctttccctgt tttccagata aacaaactca atttccatgc
    10981 cgatcagatc ctgattcgac gcaatgatgt ccagattggg aagagcggtc tgcacaatga
    11041 cggattccag gggcgcatgt ccgatcatgg catggtaaaa gtttgctttt tcataccgca
    11101 tccgttcgat gccgaggccg ctggtggagt tgccctgtgc gtcggcatca atgagcaaag
    11161 ttttccggtt ggccgcggcc agcgaggcgg acaaattaac cgctgtcgtc gttttgccga
    11221 cgccgccttt ctggtttgcg atgcatatga ttttactcat tattttttgt aattctttcg
    11281 aaaattgctt ctggaaaaaa cttgcgtgaa aactaacaca aaaaagggtt ttttagaagg
    11341 gttttttgct ttattttgcc ttttctaaaa taataatttt gcgcggcggg cccaaaagcg
    11401 gcaaatttat atcctcttga tataattgaa atatattgcc atgattttct ccagaaaagg
    11461 cggcatccac ctccggggag acgtccggac cttttaaggt tatcaacagg ccgccgggcg
    11521 cgagaaaata ctgtcccaag ggcattaaat caggcagctt gagagaggcg cgtgagatca
    11581 ccacatcaaa tttttccctc cactgcccct cacggacaat gttttccgtc cggtcctgga
    11641 gggtgaaaac accgtccaaa ttcaacagac gttgaatatg cttcaggaaa gatacttttt
    11701 tccggttgga ttccagaagg caaagttgta tagacggcaa ggcgattttc agcggaagac
    11761 cggggaatcc ggcgccggtt ccgatgtcca ccatgcgggc gttttcgaaa cggatatatc
    11821 tggcggcggt cagggagtcc agaaaatgac gggtcgcgat ttcttcagcc gtttttttgg
    11881 agatcagatt ggttttggcg ttccactgca agagctcttt ttgataaaga tcaaaccggg
    11941 cgagctgctg cccgtcgagt tcaatgccga ggaggcgcgc gccctgctga agcaaaagca
    12001 tgtcctggtt catgccggag agaaataaca aaaaacgg
//
LOCUS       DAOSRM010000067        12001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5292_length_12001_cov_106.470785, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000067 DAOSRM010000000
VERSION     DAOSRM010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5292_length_12001_cov_106.470785"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1578)
                     /locus_tag="PLA58_07290"
     CDS             complement(4..1578)
                     /locus_tag="PLA58_07290"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOR62635.1"
                     /translation="MPQKIRAEELILSIDAGTQSIRAALVDMEGNILDIVKTPIQPYF
                     SENPGWAEQQPDYYWDVLCKTTGQLLQREEPLKKNIKGVTLTTQRATMINVDKNGKPL
                     RPAIVWLDQRKADSRQIMPGALRPMIKTVKLLETLEGAIQDCDANWIRQQQPEIWEKT
                     YKYLVLSGFFTHRLTGEFIDSVANNIGYLPFDNKTYQWAGKFDFKWWIFKIGKEKLPD
                     LIKPSEVLGRITRKASAETGIPEGLPVFAAGSDKGCEILGSGCLTPETGCLSFGTIAT
                     FDVATPKYIELYPMIPPFPASVPDAYYTEVSIFRGFWMVSWFKEEFGLQECMLAEKTA
                     EPPEKFFDSLIQHVPAGSKGLMLQPYWTPGREIDPFAKGSIIGFGDVHSRAYVYRALL
                     EGLVYGLREGGELTQRKTKVPVTRLKVSGGGSQSDAAMQITADIFGMAAERPHTFETS
                     ALGAAIDAAVGLKLYPDFPAAVKAMTRTGRVFEPILKNHQIYDQLYKRVYLKMYERLQ
                     PLYKEIRDITGYPATL"
     gene            complement(1643..3328)
                     /locus_tag="PLA58_07295"
     CDS             complement(1643..3328)
                     /locus_tag="PLA58_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOR62636.1"
                     /translation="MKSSPYRPEWTEKAPPPGTYRSIFKYGDPHRFKHPSDAWYNMIK
                     QEFGLSDQDFSSRSREGLEPVSLQRPPNLSSHQIEFLQKIVGGENVALDDYSRVKYAS
                     GKTTEEMLEMRHGIIREVADVVVHPRDKEDVQKIVDYCNREKIPITVFSAGSSVNFGC
                     RPVRGGISLVVSTHMNKLLDVNELNQTARVQPGMFGPAYENALNQAPELFQTKHRYTC
                     GHFPQSFEYSTVGGWIVTLGSGQASTYYGDAYDIVFSQEYITPAGTFKTLDFPATATG
                     PKVNDIMKGSEGTFGILVEVTMKIFRYRPENRERFSFMYPTWDAAVDASREIMQSEFG
                     KPAIYRISDPEETDRGLKLYGMPGLADTLLNWRGFKPMQRCLCLGNVEGDRDYTKLVA
                     RKIKAVARRYGAISLTGYAARKWEHTRYTEPFMREDLGDYDILIDTLEAAVTWDNLHR
                     VHQGVRACVKNRPATMCLTHASHFYPQGTNLYFIFIVHMKDVGEFRKFQRGIIDSIQH
                     HGGSLSHHHGVGRMIGPWMERHLGKEQMDVLRALKKHFDPQGIMNPGGQLGLD"
     gene            complement(3374..5020)
                     /locus_tag="PLA58_07300"
     CDS             complement(3374..5020)
                     /locus_tag="PLA58_07300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOR62637.1"
                     /translation="MERFIEKNPHEKFDVIVIGGGISGASVAYEAATRGLKVALLEKK
                     DFSWATSAATSKMIHGGLRYLVNGEVRLVRESLRERRVLENIAPNYVYPQPIMMMHHK
                     KPLKNNKRVVKVGMLLYDALSYDKNRTWDPCKRIPAHKTISRREVLRQEPHVRAEGLA
                     GASVFCDCMSIFPERLTLAFIKSAVAHGAKAANYAKVEGFLMDAGNRVAGVKVKDLLT
                     GEVHDIAGTVTVNCGGPWADLLLDMAKPDGKDAPMLRRSEGIHIITAKRLLSGQYIVG
                     SMTPSGRHFFLIPWRNHTLIGTTDKPFNGNPDDYRVTRESIMELIDDVNRSFGDGKLS
                     YEDVKHTYGGLRPLVEKETRETYTSSRKYEIYDNKDAGLDGLITVEGGKYTTSRRLAE
                     NCLKIVAVKLGRNLGKSVTNKKFLTGCEIKDLNFFMNEARAQDNGLSPATLEYLARNY
                     GTEYREIAKLAREDQSLSETLNNDGEIQAQVVYAVRNEMARTLSDIVMRRTGIGTLGN
                     PGEEVLRKVADVAARELRWDKEKTEKEIAAVVHLLKIPVD"
     gene            complement(5035..5742)
                     /locus_tag="PLA58_07305"
     CDS             complement(5035..5742)
                     /locus_tag="PLA58_07305"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOR62638.1"
                     /translation="MVQKNFASPVTRSRLHEEIVTVIQKQIMSGQIKAGDKLPTEREL
                     SESFHVNRATVREALRKLENLDLLEIRHGDGLYAKNYLESGNFDLIKVAFTMDGGQKV
                     MMNILEARLYLVPQMAYLAAQRRTKKDLEDLKRVVEDQEMSMLEKDIKVHQVIARSTQ
                     NLLCTIGLNVFNQIFRDYGYLYFDSPKNIERSRKFHNEIYEAVKNQEAELARKIMREV
                     LVYAEKAVLASIKDKKL"
     gene            complement(6198..6851)
                     /locus_tag="PLA58_07310"
     CDS             complement(6198..6851)
                     /locus_tag="PLA58_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOR62639.1"
                     /translation="MIKAIFWDNDGVLVDTEGIFFRANQETLADLGISLGWEQFEEIS
                     LVRGGSVLQLAQKPGTEAFYKLRDKRDALYADLLTREPLVMEGVRDVLEALTGKYAMG
                     IVTSSGHEHFEIIHRRSGLLPYFQFALTMKDCSRFKPHPEPYLKAIALSGCRPEECLA
                     IEDSPRGLVSAVNAGIRCLVVPSRFTRGGDFPGAFQILNNIRELPATLEELQAGILD"
     gene            7093..7989
                     /locus_tag="PLA58_07315"
     CDS             7093..7989
                     /locus_tag="PLA58_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR62640.1"
                     /translation="MQDRQFGRVRLIFGENGGKYPFNHSLYLQGDKERVVIDPASSLE
                     KLTRLKEEGVDGVWLSHWHEDHIRYMNRFDGCSVWISERDFPPLADLKTFLDWYGIEE
                     QGARNYWKKEMLETFDYRPRQTARFLRDDQTLDLGGLSVQVIPTPGHTPGHLSFFFPE
                     EELLFLGDYDLTSFGPWYGDVYSSIADTVASIHKLKAIPARRWVAAHNTGLYEENPGP
                     LWEAYENTIYEREGKLLAFLDRPKTLAEICAAWLIYGRPREPREMYEFNERVLIGKHL
                     DDLLHRGKIVYEDGCWRKVESR"
     gene            8153..8608
                     /locus_tag="PLA58_07320"
     CDS             8153..8608
                     /locus_tag="PLA58_07320"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOR62641.1"
                     /translation="MIAYQLCIFAEDKPGQLANVTSVLAKADISIRATTISTAGKSGV
                     INLIVDDPKRAKKALDNAGLTVHLKNVLAVLIPDRPGGLDKLMQLLYREGININNACG
                     FVLECAEKAVFVVDVDQIEKTEKLVEKNGFKTLDTEALSAVEPFHYMKY"
     gene            8621..9052
                     /locus_tag="PLA58_07325"
     CDS             8621..9052
                     /locus_tag="PLA58_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62642.1"
                     /translation="MAHEDKGKYFKKHPAGTTVSDKLKEAVLKSTKENNISCKAAEKI
                     AHAAGFSPGEVGVAIDMLNINIAQCQLGLFGLDGKQKVSPALSVAPDLEAAIRKALVN
                     GRLSCAAAWEIADRLGVKRLEVGAACENLKIKVKPCQLGAF"
     gene            9138..11012
                     /locus_tag="PLA58_07330"
     CDS             9138..11012
                     /locus_tag="PLA58_07330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR62643.1"
                     /translation="MILNWQDTYKERLTDARSALSRIRRGARIFIASACGEPQLLVKT
                     LLDMGQNFADVEVIHFLDLGLTDYTSEIYTEHFRHNALFIGANARAAIKEGHADYTPI
                     FLSEVPLLMKRGSMPIDVALITVSPPDMNGYVSLGISVDITKTAASVARYVVAEVNPN
                     MPRTLGDSFIHVSEISAFVENDAPLLEFEQKAPDDIAQSIGRLIADLIDNESTIQTGV
                     GKIPNSVFPYLANKKDLGVHTETFTDGLIDLIESGVVTCRKKSLHPGKIVAAFCMGTR
                     RLYDYVHNNPLFEFRPCQYVNDPYVIAQNDRMVSINSALTVDLTGQVCSDSLGFEFYS
                     GIGGQVDFVRGSAMSNRGKSIMVLPSTTDNGAISRIVPYPSAGSGVVVTRGDIHYVVT
                     EYGIAYVHGKSIRDRAMMLINIAHPKFRDELLEAAKRQGYIYRDQTLPVVLYPKEYEI
                     TWVDKKGTSLFFRPVKATDERAIQELIYDLPPQDVYTRFFHNLKSFSHKVAMPMAAID
                     YDDKMAIVAVIGKEEPEGREKIVAIGNYANNPNTRYAEVAFTTHQDWQDRGIGTFLLQ
                     YLIRIAKEKNIEGFTADVLSRNRAMMQVFSKCGCPMTTHLDTGVYELKINFGAEEKKE
                     "
     gene            11048..11998
                     /locus_tag="PLA58_07335"
     CDS             11048..11998
                     /locus_tag="PLA58_07335"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOR62644.1"
                     /translation="METRRDFIKKSVVVCAALSLPLPGTANGQTVPREFKTRDPKRAL
                     ILCYSQTGFTSRYGKLIGCVLQEKGLVVDLADMRGFDPKRLTGYDLILVGSPVFYYDT
                     PANVSDWLAAIPKITGISVAAFVSFGGPEGNQHNALCHILHLLAEKEGIPVGMEAFRS
                     IPSYPATWDSANQRSGEHLPDEATYNRVREFAGEILEAVRQGRVLTYSSELALREIVR
                     MLPLVWLNKRGITQHTVDGAKCILCRTCEKKCPVNAIHPEKRSVDHDKCLACFGCLNN
                     CPADAVVMAYGGKKLYGFPEYLRRRKLKVLEPPEFQHCRL"
BASE COUNT         2599 a         3276 c         3282 g         2844 t
ORIGIN      
        1 attctacaac gtcgcgggat aaccggtaat gtcccggatc tctttataca gcggctgcag
       61 ccgttcatac attttgaggt aaactctctt gtatagttgg tcgtagattt ggtgattttt
      121 cagaatcggc tcaaacactc ttcccgtgcg ggtcatggct ttgaccgcgg ccgggaaatc
      181 aggataaagt ttaaggccga ccgcggcatc gatggccgcg cccagggcgg aggtctcaaa
      241 tgtatgcggt cgttccgccg ccatgccgaa aatgtccgcc gtaatctgca tcgccgcgtc
      301 gctttgcgaa ccgcctcccg acaccttgag cctggttacg gggactttgg ttttgcgctg
      361 ggtcagttct ccgccttccc ttaatccata gaccagccct tccagcagcg cccgatacac
      421 ataggcccgg ctgtgcacgt cgccgaagcc gataatcgat cccttcgcga aaggatcaat
      481 ctcccgtccc ggcgtccagt aaggctgaag catcagccct ttggaaccgg ccggcacatg
      541 ctgaatcaac gaatcaaaaa acttttccgg aggctctgcc gtcttctcgg caagcatgca
      601 ttcctgaagg ccgaactctt ccttgaacca gctcaccatc cagaaaccgc gaaaaatgga
      661 gacttccgta taataagcat ccggcacgga cgcaggaaac ggcggaatca tcggatacag
      721 ctcaatatat ttgggcgtgg ccacatcaaa cgtggcaatg gtcccgaaac tcagacatcc
      781 tgtttcgggc gtcagacacc cggaaccgag gatttcgcat cccttgtccg atccggccgc
      841 aaagacgggc agcccttccg ggatgcctgt ttccgcggaa gctttcctgg tgatccggcc
      901 cagcacttca gaaggtttga tgaggtcggg aagtttttct ttgccgatct tgaaaatcca
      961 ccatttgaaa tcgaatttcc ccgcccattg ataggtcttg ttgtcgaacg gaagatagcc
     1021 gatattattt gccaccgaat caataaactc tccggtaagg cggtgcgtaa agaagccgga
     1081 cagcaccaaa tacttatagg ttttctccca aatctccggc tgttgctggc ggatccagtt
     1141 cgcatcgcag tcctggatgg ccccttcgag cgtttcgagc agtttgacgg tctttatcat
     1201 gggccggagc gcgccgggca taatctgccg tgaatccgcc tttctctggt caagccagac
     1261 aatcgccggc cggagcggct tgccgttttt atcgacgttg atcatcgtgg cgcgctgtgt
     1321 cgtgagcgtc acgcccttga tatttttttt cagaggttct tctctttgca ggagttggcc
     1381 ggttgtcttg cagagtacat cccagtagta atcgggctgc tgttcggccc agcctggatt
     1441 ttccgagaaa tacggttgaa tgggcgtctt cacaatatcc aggatgtttc cttccatatc
     1501 gacaagcgcg gcacggatgg attgggtgcc tgcatcgatg gacaggatta attcctctgc
     1561 tctgattttt tgtggcatat gcaccgccct gtttttttaa tttattctaa tgatcgatca
     1621 tgccgcttcc cggtcgcttt cttcagtcca gccccagttg accgccggga ttcataatcc
     1681 cctgcgggtc aaaatgtttt tttaaagccc gcaggacatc catctgttcc tttccaaggt
     1741 gcctctccat ccaggggccg atcattcttc ccaccccgtg atgatggctc agagaaccgc
     1801 catgatgctg aatgctgtcg atgatccccc gctggaactt gcggaattcc ccgacatctt
     1861 tcatgtgaac gataaaaatg aaatagagat tcgttccctg cggataaaag tgcgaagcgt
     1921 gcgtcaggca catggtggcg ggacggttct tgacacaggc ccgcacgcct tgatgcaccc
     1981 ggtgaagatt gtcccaggtc acggcggctt ccagcgtgtc gattaaaatg tcatagtcgc
     2041 ccaggtcctc gcgcatgaag ggttccgtgt aacgggtgtg ctcccatttc cgcgccgcgt
     2101 aaccggtcag ggaaatcgca ccgtaccttc gggcaaccgc cttgattttc cgcgccacca
     2161 gttttgtgta atcccggtct ccttccacat tgcccagaca cagacaacgc tgcatgggtt
     2221 tgaagccgcg ccaattgagc aacgtgtccg ccaggcccgg catgccgtag agtttcaatc
     2281 cccggtcggt ttcctcggga tcggaaatgc ggtagatggc cggcttgccg aactccgact
     2341 gcatgatttc gcggctggcg tcaacggccg cgtcccaggt gggatacatg aagctgaaac
     2401 gttcgcgatt ttccggccga tagcggaaga ttttcatggt gacttcgacc agaatgccga
     2461 acgtcccttc ggatcccttc atgatgtcgt tgactttggg accggtggcg gtcgccggaa
     2521 aatccagcgt cttgaacgtt ccggcgggcg tgatgtactc ctgactgaag acgatatcgt
     2581 aagcgtcgcc gtaataggtc gaggcctgcc cggagcccag ggtgacgatc cagccgccga
     2641 cggtggaata ctcaaaggac tgcgggaaat gtccgcaggt gtagcgatgt tttgtctgga
     2701 aaagttcggg ggcctggttc aatgcgtttt cataagccgg cccgaacatt cccggttgaa
     2761 cccgcgccgt ctgattcagt tcgttcacat ccagcagttt gttcatgtgc gtgctgacca
     2821 ccagcgagat gccgccgcgg accggacgac agccgaagtt cacggaagag ccggcgctga
     2881 aaaccgttat cggaattttt tcccggttgc agtagtccac gattttttgc acatcctctt
     2941 tgtcgcgggg atgaaccacc acatccgcca cttcgcggat gatgccgtgg cgcatttcca
     3001 gcatttcctc cgtggtcttt ccggaggcgt atttgacgcg gctgtaatca tccagggcga
     3061 cgttttcccc gcccacgatc ttctgcagga attcaatctg atgagaagaa aggttcgggg
     3121 ggcgctgtaa agacaccggt tccagtcctt ccctgcttcg ggaggagaaa tcctggtcgc
     3181 ttaaaccaaa ctcctgtttg atcatgttgt accaggcatc gctgggatgt ttaaagcggt
     3241 ggggatcacc gtatttaaaa atggaccggt aggttcctgg gggcggcgct ttttccgtcc
     3301 actccggacg gtaaggactg gatttcatcg gcaggtctcc tttgttggtt ttctttgata
     3361 gatgaagcgc ttttcagtca accggtattt tgagcagatg gacgacggcc gcaatttcct
     3421 tttccgtttt ttctttatcc cagcgcagct ctctggcggc aacatcggca accttgcgca
     3481 atacctcttc gccgggattg ccgagtgtgc cgatgccggt gcgtcgcatg acgatatcgg
     3541 agagagtgcg cgccatttca ttgcgcacgg cataaaccac ctgcgcctgg atttcgccgt
     3601 cattgtttag ggtttcggac aaggactgat cctcgcgcgc cagtttggca atttctctgt
     3661 attcggttcc ataattgcgc gccagatatt ccagggtggc cgggctgagt ccattgtcct
     3721 gcgcccgcgc ttcattcatg aagaaattca aatcctttat ttcgcagccg gtcagaaact
     3781 ttttattcgt aacgctcttg ccgagattgc gccccagttt gacggcgacg atcttcaggc
     3841 agttttccgc caaccggcgg ctggtggtgt acttgccgcc ttcgaccgta atcagtccgt
     3901 ccagtccggc gtctttgttg tcgtagattt catatttgcg cgacgaggta tacgtttcgc
     3961 gggtttcttt ctcgaccaga ggacgcagac cgccataggt atgcttcacg tcttcataag
     4021 agagcttgcc gtcgccgaag gagcggttga catcgtcgat cagttccata atgctttccc
     4081 gggttacccg gtaatcgtca gggttgccgt tgaagggttt gtcggtcgtg ccgatcagcg
     4141 tgtgattgcg ccagggaatc aggaagaaat ggcggccgga cggcgtcatg gaaccgacga
     4201 tgtattggcc ggaaagaaga cgttttgcgg tgatgatgtg aattccttca gaccggcgca
     4261 gcatcggcgc gtcttttcca tccggtttgg ccatatccag aagaagatcc gcccacggtc
     4321 cgccgcagtt gaccgtaacg gttcccgcaa tatcatggac ttcgccggtc agcaggtctt
     4381 tgaccttcac cccggcaacc cggtttccgg catccataag gaaaccttcc actttggcat
     4441 aattcgccgc tttcgcgccg tgggccacgg cggatttgat aaaggccagc gtcaggcgtt
     4501 cgggaaaaat gctcatgcag tcgcagaaga ccgatgcgcc ggccagtcct tcggctcgaa
     4561 catgaggctc ctgccgcagg acctctctgc gggaaatcgt tttgtgcgcc ggaattcgtt
     4621 tgcagggatc ccaggtgcga tttttatcat aggacagggc gtcgtaaagc agcatgccca
     4681 ctttcacgac ccgtttattg ttcttcaacg gctttttatg atgcatcatc atgatcggct
     4741 gcggatagac gtaattggga gcgatatttt ccagcactct tcgctcgcgc agcgattccc
     4801 gcaccagcct cacttctcca ttgaccagat accgaagacc gccgtgaatc attttggacg
     4861 tggccgccga agtcgcccag gaaaaatcct ttttttccag cagcgccacc ttcaatcccc
     4921 gggtggcggc ttcataggca accgacgccc cgctgatccc cccgccgatg acgatcacgt
     4981 caaatttttc atgaggattc ttttctataa accgttccat gcccgccgct ccttttaaag
     5041 ctttttgtcc ttgatgcttg ccagaaccgc cttttccgcg taaacgagaa cctctctcat
     5101 gatttttcgg gccagctccg cctcctgatt tttgacggcc tcgtagattt cgttgtgaaa
     5161 tttccgggag cgctcgatgt ttttggggct gtcgaaatag agatatccgt aatcacggaa
     5221 tatttgattg aaaacgttca acccgatggt gcaaagcaga ttttgggtgg agcgggcgat
     5281 tacctggtga accttgatat ccttctccag catactcatc tcttgatctt caacgacgcg
     5341 tttcaggtct tccaggtctt ttttggtacg cctctgcgcc gccagatagg ccatctgcgg
     5401 caccagatag aggcgcgcct ccagaatgtt catcatcacc ttttggccgc cgtccatcgt
     5461 aaaagcgacc ttgatcaggt cgaagtttcc gctttccaga taatttttgg catacaaccc
     5521 gtcgccgtgg cgaatttcca gcaggtcgag attttcgagt ttgcgcagcg cctcgcgcac
     5581 ggtggcccga ttgacgtgaa aactttccga aagttccctt tccgtgggta atttgtcgcc
     5641 cgcctttatt tggccggaca taatctgttt ctggatcacg gtgacaatct cttcgtgaag
     5701 gcgcgaacgg gttaccggac ttgcaaaatt cttctggacc atcgggatca acgctccttt
     5761 tccctcccgc ctaattggcc gggtggttcg accaataata tattccatcc aaaaggtcaa
     5821 gaggtttgtt tttgggctga gaactttgca caattacact tttgtcatcg cgaggagcga
     5881 agcgacgtgg cgatctcttc aaatgatcaa atgctgatga gatggcttca cttcgtttgc
     5941 aatgacataa gagacattat gcaaaggttt ttgtgctgtg tcgcaattag aaaatcatac
     6001 cgaggggaat tacgtaggga tcggtccccg accgatccgc aagaccgcgt cacaatggat
     6061 catggttttt gactctgctt gcgctctccc cttctctttt ggaagggtcg ggggtagagg
     6121 caactccaag tgatggtaat cgagatctcc cctcctcttg tgggtgggag gggcaggggg
     6181 agggggaatc ttgagcatta atccaggatt cccgcctgca attcctccag cgtcgctggg
     6241 agctccctga tattgttcag gatttgaaag gcgcccggaa aatcgccgcc ccgggtgaat
     6301 cggcttggaa ccaccaagca ccggatcccg gcgtttacgg cggagacaag accgcgcggc
     6361 gaatcctcaa tggcgaggca ctcctccggc cggcagccgg aaagcgcgat cgctttcagg
     6421 tacggttcgg gatgcggctt gaaacgcgag cagtctttca tggtcagggc aaattgaaaa
     6481 taaggcagaa gccccgaccg ccggtgaatg atctcgaagt gctcatgtcc ggaactggtt
     6541 acaattccca tggcgtattt tcccgtaagc gcctccagca catcccggac gccttccatg
     6601 accagcggct cccgcgtcag caggtctgca taaagagcgt cccgcttgtc gcgcagttta
     6661 taaaacgcct ccgtccccgg cttttgtgcc agttgcagca cactcccgcc ccggacaagc
     6721 gaaatttctt caaactgctc ccatccaagc gaaatgccca aatccgccag cgtctcctga
     6781 ttggccctga aaaagatgcc ttccgtgtca accagaacgc cgtcattatc ccagaaaatt
     6841 gccttgatca tcgacctgac ctgatgctcc ttatccccaa ctccctggcc gccgctcgcc
     6901 ttttccgcta cggcctatta tcattggatg cgttgtagtg gaaaaccaat gatctcgtgt
     6961 caaggagtta acacaagata cagtaaatgg aaaaacaaat tgtttcaatg agttttcgct
     7021 tatggaatta ttgttggcaa tttggccgga tattttctat attcaaccat catcatactg
     7081 ggggcgttag acatgcagga tcgtcagttc ggcagggttc gtttgatctt cggtgaaaac
     7141 ggcggcaagt atcctttcaa ccattcgctc tatctgcagg gcgataagga gcgggtggtg
     7201 atcgatcccg cttcttcact ggaaaagctg acgcggttga aggaagaagg cgtcgatggg
     7261 gtctggcttt cgcattggca cgaagatcac attcgctaca tgaaccgctt tgatggttgt
     7321 tccgtttgga tctccgaacg cgattttccg ccgctggccg atctaaaaac gtttctggac
     7381 tggtacggca ttgaagagca gggcgcccga aactactgga agaaggaaat gctggaaacg
     7441 tttgattacc gcccccgcca aacggcgcgt tttctccggg atgaccagac gttggatctg
     7501 ggcgggcttt ccgtgcaggt cattcccacg cccggacata cgcccggaca tctgtcgttc
     7561 tttttccctg aagaggaact gctgtttctg ggggattacg atctgacgtc gttcgggccg
     7621 tggtatggcg atgtttattc cagcattgcc gataccgttg catcgatcca caagctcaaa
     7681 gcgataccgg ccaggcgctg ggtggcagcg cacaacacgg gcctgtacga ggaaaatccg
     7741 gggcctctct gggaggctta cgaaaatacg atttatgagc gggaagggaa gctgctggct
     7801 tttctggacc ggccaaaaac gctcgctgag atctgcgcgg cctggctgat ttacggcagg
     7861 ccccgcgagc ccagggagat gtatgaattc aacgaaaggg ttctgatcgg aaagcatctg
     7921 gacgatttgc tccacagggg taaaatcgtt tacgaagacg gctgctggcg gaaagtcgaa
     7981 tcccgttgat gcccgacgag cgttcatcct gcgcaacttg ctgcgatatc atgatgttcg
     8041 tttcatgcct tgacagcttg ccgcgaggtg gttgattccg agatttgact tgataaaaca
     8101 cgggcatcat tgtaaaaaag actcaaacgg caaagaaaat caggagaaga acatgattgc
     8161 ttatcagctt tgtattttcg cggaagataa accggggcaa cttgccaacg tgacgagcgt
     8221 tctggcaaaa gccgacatca gcatccgggc gaccaccatt tcgactgccg gcaagtccgg
     8281 ggtgatcaac ctgattgtgg atgatccgaa gcgcgcgaaa aaggctctcg acaacgctgg
     8341 gttaaccgtt cacctgaaaa acgttctggc cgtcctgatt cccgaccggc cgggcgggct
     8401 ggacaagttg atgcagcttt tgtacaggga aggcatcaat atcaacaacg cctgcggctt
     8461 tgttctggaa tgcgcggaaa aagccgtgtt tgtggtggac gtggatcaga ttgaaaaaac
     8521 ggaaaaactt gtggaaaaga acggattcaa gacgctggac accgaggcgc tgtcggcggt
     8581 ggagccgttc cattacatga aatactgatg ggggaaaacc atggcgcatg aggataaagg
     8641 caaatatttc aagaagcacc ccgcagggac aaccgtcagc gacaagctga aagaggcggt
     8701 tttgaagtcc acaaaggaaa acaacatttc ctgcaaggcg gcggaaaaga ttgcccatgc
     8761 tgccggtttt tcgccgggtg aagtcggcgt ggcgattgac atgctcaata tcaatattgc
     8821 gcagtgccag ctcggcctct tcggactgga cggcaagcaa aaagtgtcgc ctgctctatc
     8881 cgtcgctccg gaccttgaag ccgccatccg caaagcgctg gtcaacggcc ggttgtcctg
     8941 cgctgcggcc tgggaaattg cggaccggct gggcgtcaag cgcctggagg tcggcgccgc
     9001 ctgtgaaaac ctgaagatca aggtcaaacc ctgtcagctc ggcgcctttt agcggatttc
     9061 gggagcggtt cgattgcggc ggggatatta gaaacggctg atttcctccg tttgtctcat
     9121 cagggaaaat acccgatatg attctcaact ggcaggatac ctacaaggaa agactcaccg
     9181 acgcgcgaag cgcgctgtcc cgaatccggc gcggcgcgcg catcttcatt gcttccgcct
     9241 gcggcgagcc gcagcttctg gtcaaaacgc ttctggatat ggggcagaac tttgccgatg
     9301 tggaagtgat ccactttctg gatctggggc tcaccgacta cacgtcggaa atctacacgg
     9361 agcatttccg gcacaacgcg ctcttcatcg gcgcgaacgc ccgcgcggcc atcaaggaag
     9421 ggcacgccga ctacacgccc attttccttt ccgaagttcc cctgctgatg aagcggggat
     9481 ccatgcccat tgatgtcgcc ctcattacag tctctccgcc ggacatgaac ggctatgtca
     9541 gcctggggat ttccgtggac atcaccaaga cggcggcttc cgtggcccgg tatgtggtgg
     9601 cggaggtgaa tccgaacatg ccgcggacgc tgggcgacag ttttatccat gtgagcgaga
     9661 tttccgcgtt tgtggaaaac gacgcgccgc ttctggaatt cgaacagaag gcgccggatg
     9721 acatcgctca gtcgatcgga cggctgattg ccgatctgat cgacaatgaa tccaccattc
     9781 agacgggagt cggcaagatt cccaattccg ttttccccta tctggccaat aaaaaagacc
     9841 tcggcgtgca cacggaaacg tttaccgacg gcctcatcga tctgattgaa agcggcgtcg
     9901 tgacctgccg gaagaagtcg ctgcatccgg gaaagattgt cgcggccttc tgcatgggca
     9961 cccgacgcct gtatgactat gtgcacaaca atccgctctt tgaattccgg ccgtgtcagt
    10021 acgtgaacga tccgtatgtc atcgcccaaa acgaccggat ggtgtccatc aattccgcgc
    10081 tgaccgttga tctcaccggg caggtgtgtt cggattccct cggattcgag ttttacagcg
    10141 gcatcggcgg gcaggtggat tttgtccgcg gttcggccat gtccaatcgg ggcaaatcca
    10201 tcatggtgct gccttcgacc acggataacg gggccatctc ccgcatcgtg ccgtatccct
    10261 ccgcgggcag cggcgtggtg gtgacgcggg gggacatcca ttatgtcgtg acggaatacg
    10321 gcattgccta cgtgcacggg aaatccatcc gcgaccgggc catgatgctc atcaacatcg
    10381 cccatccgaa gttccgcgac gagctgctgg aagccgccaa gcgtcagggc tacatttacc
    10441 gggatcagac gctgccggtg gtgctgtatc cgaaggagta tgaaatcacc tgggtggaca
    10501 agaagggaac ctcgctgttt ttccgtccgg taaaagccac ggacgaacgc gccatccagg
    10561 aattgatcta cgatctgccg ccgcaggatg tctatacgcg gttctttcac aatttaaagt
    10621 ccttttccca caaagtggcc atgccgatgg cggccatcga ttatgacgac aagatggcca
    10681 tcgtggcggt catcggcaag gaggagccgg aggggaggga aaagatcgtg gccatcggaa
    10741 actacgccaa caatcccaac acccgctatg ccgaagtggc cttcaccacg catcaggact
    10801 ggcaggaccg gggcatcggc accttcctcc tgcagtattt gatccgcatt gcgaaggaga
    10861 agaacattga aggattcacg gcggatgttt tgtcgcgaaa ccgggcgatg atgcaggtat
    10921 tttccaagtg cggctgtccg atgaccacac acctggatac cggagtctat gaattgaaga
    10981 tcaacttcgg cgctgaagaa aagaaagaat aaccttcaag cacatcacaa tcgtcggatc
    11041 gaattctatg gaaaccagaa gagacttcat caaaaaaagc gttgtggtct gcgccgcctt
    11101 aagccttccg ctgccgggga ccgcaaacgg acagaccgtc ccccgggaat tcaaaacccg
    11161 cgatccgaaa cgcgcgttga ttttgtgtta cagccagacg ggatttactt cccgctacgg
    11221 gaaactgatc ggctgtgttc tgcaggaaaa agggcttgtg gtggatcttg cggacatgcg
    11281 cggttttgat ccgaaacgcc tgaccggcta cgatctgatc ctggtcggct cgccggtttt
    11341 ttattatgac acgccggcca atgtgagcga ttggcttgcc gcgattccga agattaccgg
    11401 aatttccgtg gcggcgtttg tgtcgttcgg cggaccggaa ggcaatcagc acaatgcttt
    11461 gtgccacatc cttcatctcc tggcggagaa agaaggcatt cctgtcggca tggaagcttt
    11521 ccgcagcatt ccatcctacc cggcaacctg ggacagcgcc aatcaacgct ccggagagca
    11581 tctgccggat gaggccacat acaaccgggt tcgggagttc gccggggaaa tcctggaagc
    11641 cgtgcggcag ggccgggttc tgacctattc ctccgagctg gcgcttcggg aaatcgtgcg
    11701 gatgcttccc ctggtgtggc tcaacaaaag aggaatcacc caacataccg tggacggagc
    11761 gaaatgcatt ttatgccgga cctgcgaaaa aaaatgtccg gtgaacgcca ttcacccgga
    11821 aaagcgttcg gtcgatcacg acaaatgcct ggcctgcttc ggctgcctga acaactgccc
    11881 ggcggacgcc gtcgtgatgg cgtatggcgg caaaaaactt tacggtttcc cggaatatct
    11941 gcggcgcagg aaactgaagg tcctggagcc gccggagttt cagcattgca ggctttgaag
    12001 a
//
LOCUS       DAOSRM010000068        11923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5342_length_11923_cov_106.229103, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000068 DAOSRM010000000
VERSION     DAOSRM010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5342_length_11923_cov_106.229103"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..3001)
                     /locus_tag="PLA58_07340"
     CDS             complement(23..3001)
                     /locus_tag="PLA58_07340"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOR62645.1"
                     /translation="MTPIHVLQPTLHSGDVVQGFIVLRVEVIESMRLTAYEMEHRKTG
                     AKVLHLHAMDRENLYAIGFRTPPADSTGLPHILEHCVLAGSEKYPLKDAFKELIRSSM
                     QTFINAFTYPDKTLYPVASQIKADFYNLARVYTDLVLHPRLLRETFLQEGHHLEFAKP
                     GDPESDLIISGIVYNEMKGAYSSPDSLMYKDIQQNLYPDTVYALDSGGDPDIIPTLTY
                     EQFRDFHRKYYSPTNARFFLYGDIPTADHLEFLAEMLAGFERVEVDSSITSQPFFSGP
                     RRVAGVYPIDPDEPSEKKTNVNLAWMLAENKDFETALTLSILSGLLVGSAASPLRRAL
                     VDSGLGEDLSPVSGIESDLKQMMFCAGLRNIKNADIPNVEKLILDTLQKIVAEGFDPE
                     LIEGILHQVEFHGKEISRGSYPYGITLMGGVFQTWLYDGDPLVGLDFSRVIEKIRLLL
                     KDDPQIFQKVAQQWLVDNPHRLLAVMEPDPDFNRKSEAELTKKMAALKAGLSKDQLRA
                     IDEQAARLKAFQSEEDSSKAQASLPKLKMEDLPRQVETVPTRHKTLAATPALAHDLFT
                     NGIAYVDLAFDTASVPESLQIYLPLFGKIVSGMGAAGLTYDEMAKRIALQMGGFGYDL
                     SSGLSVDAARVWQKMVFSFKALYRNVPQAVRTVRDILCFGDLNDESRMRDLIAERKNN
                     LIASVVPSGHVFAKRAAAAGLTLPAYRDEQWHGRTQLRFVQRQAEGFDASKKPLREKL
                     EQLREILFTRQNLTVNMTADADGLTLVETPIGELLESLPAGRRSEEPERPTLAPVRTG
                     IAVPTQVSYVASAMKAPAYTDPSSALIMLASRELSNTYLYKHIRVQGGAYGGMSSFDP
                     SQGIFAFLSYRDPHIVETMNVFEAAQKYYSKNEMTKDDLEKAVISALGALDKPLDPSG
                     RGYVALFRHFSGATDDLRQAFRDRLTAATPSQVRDALSDYFEKASPSGSVAVYSSQEK
                     LEEANRRLEKKLKLEKLSEA"
     gene            complement(3025..4188)
                     /locus_tag="PLA58_07345"
     CDS             complement(3025..4188)
                     /locus_tag="PLA58_07345"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HOR62646.1"
                     /translation="MKRSLLFPFFPLLAALLFFCAAVEAAVIPVTKFRSADLPGGAPP
                     GWALEKKTGAPSLKISKEGDNYYLHLRSQGNSSFGVRTSARVNVKDFPILSWRWKVEK
                     MPVGGDVRKKTADDQALQVYIAFKETGFPAALNTPVVGYIWDNEAPKGWSGRSTQIGG
                     DKMRYIVLRNKTDKIGQWYTERRNLYEDYKKLFGDINGGEPLGVTTGLQIHINTQRTR
                     TPAEGLIGEIYFSSETKDVVVTEAAKEKMVAATPVLSSPRGKALPVAGFDDDFNRPGC
                     VNISVAFDTNSTQVDAIPKSSVQTIMEYLVKHPENTLVITGHTDSVGSDAFNLELSRR
                     RAQSVKQYLVEQYAIDEHRLIILGAGESRPIADNDTPEGQAQNRRVTIQDCPR"
     gene            complement(4185..4787)
                     /gene="pyrE"
                     /locus_tag="PLA58_07350"
     CDS             complement(4185..4787)
                     /gene="pyrE"
                     /locus_tag="PLA58_07350"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOR62647.1"
                     /translation="MMSMDQSTAKKRLGKIILERSFKYSDNPPFTLASGRQSNFYFNC
                     KPTTLDPEGMNLIGEIVFDMLKDAPVTAAGGLTLGADPIANALAVISFQRGKPIKSFI
                     VRKDVKAHGTKSAIEGNVAAGERVAIIDDVITTGGSTITAIEQARRAGLVVDRVITLI
                     DREEGGRENILQRADCVESVFTRTQIMALREEILSGQQRT"
     gene            complement(4811..5527)
                     /locus_tag="PLA58_07355"
     CDS             complement(4811..5527)
                     /locus_tag="PLA58_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOR62648.1"
                     /translation="MTTETFNRFLSIGASVGLHALAAAVLIFGLSGPATVPEMTGRLD
                     VVWASIASGKDASSSPAPRVGQKKPSAAEGRSSATGEMQTAQNFQPEFSTAVSADEKA
                     AADAATTFFGTPKAVYAEAPGHGFASDAARAQAGPVAQPVVSNAYPRYRENPPPGYPE
                     TARQRGYEGVVLVAVEILTDGRVGKAVVRQSSGYAILDHTAVAAVKDWKFVPAKKSGV
                     PYKTWAELPIKFVIHENTRS"
     gene            complement(5533..7932)
                     /locus_tag="PLA58_07360"
     CDS             complement(5533..7932)
                     /locus_tag="PLA58_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOR62649.1"
                     /translation="MTKSRKIKSLIQKQLLILDGATGTELQKRGLPAGVCPEIWCLEN
                     PTVIQDIHASYAKAGAQVVYTCTFGANRFKLKQFGVKKDVCSVNRELARLARLACGKK
                     ALVAGDIGPTGLFIEPFGPLAFEEAVDAFKEQARGLIDGGCDLIVIETMIDIQEARAA
                     LLAVKELADIFTIASMTFENDGHTLGGTPPEAALITLQSLGADAVGCNCSTGPEQMAR
                     SIAAMKPYATVPLVAKPNAGLPRLEGLKTVFDMDAKTFAASAKKLIAAGANLLGGCCG
                     TTPAHIAALAGAVGKRKPAKPRRTSLAALSSARSHLPLDEDQPLLVVGERINPTGKKA
                     LQQELMEGKLSIIRQMAQEQEIQGASLLDVNIGVPGIDEVATIKKVIGLLAATTRLPL
                     VIDSSRVETIEAALRLYPGRMLINSISGEKEKLARLLPLAAKYGAMFILLPLTDGNIP
                     KTAPKRQAVIRSIYAKAQKAGMTKEDIIVDGLTMAVASDKDAARETLATVAWCKKTFK
                     SRTVLGLSNVSFGMPGRPWLNAAFLAMAQYCGLSMAIANPAGVEFMNVKKAGDALVAR
                     EKAPLLFIRHFSEQAPAAPQTASAQMSPEEKVTAAVLDGDRDRIVSFVEALLQAGLPA
                     SRIVDEVLIPAIVRVGNLYEKKIYFLPQLMAAAETMKKALALLDPYLKQKAAAEKGKV
                     LLATVRGDIHDIGKNIVALLLRNYGYCVIDLGKDVQDQAIIEAAQKERPDVIGLSALM
                     TTTMVHMKEIISLARAKGIRTDFLVGGAVVTQAYAESIGASFARDGVAAVKAVEQLIN
                     K"
     gene            complement(8032..9075)
                     /locus_tag="PLA58_07365"
     CDS             complement(8032..9075)
                     /locus_tag="PLA58_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62650.1"
                     /translation="MLKSSEALDIKPDKIPVVLLYNVDPDWTDAEKEEVIGQSTKLGL
                     ALIESGYPTVFASIADDDLEGCLGRFDPASHIVFNWCESLPGISHSEWLVAKRLEAMG
                     FTFTGAGSETLALAQDKAAVKALLDAAGIPTPAWRIFQAGEAVRWERFPAIVKPVNEH
                     CSAGISRDSVALDAVELAGRIPFILETYRQPALVEDFIDGREFHVSVWGNEILTVLPV
                     AEMDFAFFKDIRDRLCGHDAKFIPGSDAYEKIETRLPAPLDPEEKTALENVCCDAYRA
                     LGCRDYARMDVRLRDGVFYILDINPNADISHDASMACAAEAAGISYGQMGGAIVRMAA
                     RRHPVFRQLTITS"
     gene            9186..9542
                     /locus_tag="PLA58_07370"
     CDS             9186..9542
                     /locus_tag="PLA58_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOR62651.1"
                     /translation="MKNTKPMECLRCGRCCLADFTAYATEEDIRRWKAEGRDDILEVL
                     ERGQAFWEGDRLMSRESGTPLAGCRFFAFDGERYFCAIHETRPAVCRLYEPGSSEICP
                     QHSRLGKGNPRKRIRG"
     gene            complement(9586..10032)
                     /locus_tag="PLA58_07375"
     CDS             complement(9586..10032)
                     /locus_tag="PLA58_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62652.1"
                     /translation="MEAQKDFKELLELFNGHKIEYMIVGAYALAYHGAPRFTGDIDIL
                     IKPSTENAQRILCALAEFGFGALNLTIEDFQNPDSVVQLGVPPVRIDIMTSITGVPWE
                     KADKGKVKGLYGDIPVHFLGRKEYIANKRAMGRKKDLADLESLGEE"
     gene            complement(10742..11089)
                     /locus_tag="PLA58_07380"
     CDS             complement(10742..11089)
                     /locus_tag="PLA58_07380"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOR62653.1"
                     /translation="MSKNYLKYKKYVGSVQFDADDRIFHGHVLGINDAISFEGSSVEE
                     LEQDFKAAIENYFETCRKIGKEPEKPFKGAFNLRLDPSLHELLVAGALSEGKTLNAFV
                     KDVLKKAVSVTHA"
     gene            complement(11086..11406)
                     /locus_tag="PLA58_07385"
     CDS             complement(11086..11406)
                     /locus_tag="PLA58_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOR62654.1"
                     /translation="MICNRFLVFSLPFLFPSGTLSYVNKKQRKTLADIYEKPTRCDVS
                     WRAVESLFKAIGADIFEGRGSRVSVVFDQRVLDIHKPHPSGEMKKYAVEKIRDFLAVI
                     GVKP"
     gene            complement(11495..11570)
                     /locus_tag="PLA58_07390"
     tRNA            complement(11495..11570)
                     /locus_tag="PLA58_07390"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11535..11537),aa:Ala,seq:cgc)
     gene            complement(11626..>11923)
                     /locus_tag="PLA58_07395"
     CDS             complement(11626..>11923)
                     /locus_tag="PLA58_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62655.1"
                     /translation="RFLPSVEMTKMAFIQSSHFVLWFDGQNCSVKRLISRLYDASDTL
                     RHGCEYREKALVLSRKVWKTKRPAATGSGRHSADQQQRKVREGSAHQPTPPQRN"
BASE COUNT         2444 a         3326 c         3396 g         2757 t
ORIGIN      
        1 cttcagcctt cagccttttc ccctatgctt ccgacaattt ttccagtttc agcttctttt
       61 ccagacgccg gttggcttct tccagttttt cctgcgagga ataaaccgcg accgagccgg
      121 atggcgaggc tttttcaaaa tagtcggaca gggcgtcccg cacctgactg ggcgttgcgg
      181 cagtaagccg gtcccggaag gcctggcgca ggtcgtcggt cgcgccgctg aaatgccgga
      241 acagggcgac ataaccgcgc cccgaaggat cgaggggctt gtccagcgcg ccaagcgcgc
      301 tgatcacggc cttttccaaa tcgtcttttg tcatttcgtt tttggaataa tatttttgcg
      361 cggcctcaaa gacattcatc gtctccacga tgtgcggatc gcgataggac agaaatgcga
      421 atatgccctg cgacgggtcg aaagacgaca ttcccccgta ggcgccgccc tgcacgcgga
      481 tatgtttgta cagatacgtg ttggatagtt cccgcgatgc cagcatgatc agcgcggagg
      541 acggatcggt gtaggcgggt gctttcatcg ccgaggccac ataggaaacc tgggtgggaa
      601 ccgcgatgcc cgtgcggacg ggcgcaagcg tgggcctttc gggttcttcg ctccgacggc
      661 cggccggcag gctttccaga agttcgccga tgggtgtttc caccagcgtg aggccgtctg
      721 catcggcggt catgttgacc gtcaggtttt gcctcgtgaa gaggatttcc ctgagctgct
      781 ccagcttttc gcgcaggggc tttttggagg cgtcgaagcc ctccgcctgc cgctggacaa
      841 atcgcagctg cgtccggccg tgccattgct catcgcgata ggcgggcagc gtcagccctg
      901 ccgccgccgc ccgtttggcg aaaacgtgtc cggaaggaac gacggaggcg atcaggttgt
      961 ttttccgctc ggcgattaaa tcacgcattc tcgattcatc gttcaaatcc ccaaagcaga
     1021 gaatgtcgcg gacggtccgg acagcctgcg gaacattgcg gtagagcgct ttgaaggaaa
     1081 agaccatttt ctgccagacc cgggccgcgt ccacggaaag gccggaagac aggtcatagc
     1141 cgaatccgcc catctgcagg gcgatgcgtt tggccatctc gtcgtaggtc agacccgccg
     1201 cgcccatgcc gcttacgatt ttgccaaaaa gcggaaggta gatctgcagg ctttcgggaa
     1261 ccgaagcggt gtcaaacgcc agatcgacat aggctatgcc gttggtaaac aggtcatgcg
     1321 ccagcgcggg ggtcgcggcc agagttttgt gccgggtcgg aaccgtttcc acctgccggg
     1381 gaaggtcttc cattttcagt ttgggcaggc ttgcctgggc cttcgatgaa tcctcctcgg
     1441 attgaaaggc cttcagacgc gccgcctgct cgtcgattgc ccgcagctga tctttcgaca
     1501 aaccggcttt gagcgcggcc atcttcttcg ttaattcagc ttcgctcttc cggttgaaat
     1561 cgggatccgg ctccatcacc gccagcaggc gatgcggatt gtccaccagc cattgttggg
     1621 ccactttctg gaagatctgc ggatcgtctt tcaggaggag gcggatcttt tcaatcacgc
     1681 gcgagaaatc gaggccgacc aggggatcgc cgtcgtagag ccaggtctga aaaacgccgc
     1741 ccatcagcgt aatcccgtaa gggtaggagc cgcgggaaat ttccttgccg tgaaattcga
     1801 cctgatgcag gatgccctcg atgagttccg ggtcgaatcc ttcggcgaca atcttttgca
     1861 gggtgtcgag gatcagcttt tcaacattcg ggatgtcggc gtttttgatg ttgcgcagac
     1921 cggcgcagaa catcatctgc ttgagatcgg attcgatgcc ggacacgggc gacagatctt
     1981 ctcccagccc cgaatcgaca agcgcccggc gcaggggcga cgcggcgctg cccacgagca
     2041 ggccggaaag aatggacagc gtaagcgccg tttcaaagtc tttgttttcc gcgagcatcc
     2101 aggccagatt gacgttggtt ttcttctccg aaggctcatc cggatcgatg ggatagacgc
     2161 cggcaacccg ccggggaccg gaaaagaaag gctggcttgt gatggacgaa tccacctcca
     2221 cccgctcgaa gccggccagc atttcggcca gaaattccaa atggtccgcg gtggggatgt
     2281 ccccatagag gaaaaagcgc gcgttggtcg gcgaatagta tttgcggtgg aaatcccgaa
     2341 actgttcata ggtcagcgtc ggaatgatgt ccgggtcgcc cccggagtcc agggcataca
     2401 cggtgtccgg ataaagattt tgctgaatgt ccttatacat cagcgaatcg ggcgatgaat
     2461 aggcgccctt catttcattg taaacaatgc cggagatgat cagatcgctt tcgggatcgc
     2521 cgggctttgc aaattccaga tgatggcctt cctgcaggaa ggtttcgcga agcaggcggg
     2581 gatgcagaac aaggtccgtg taaacgcgcg ccagattata aaaatccgcc ttgatctgac
     2641 tggctaccgg gtaaagggtt ttgtccgggt acgtgaaggc gttgatgaag gtttgcatgc
     2701 tggaacgaat cagttctttg aaggcgtcct tcagcggata tttctccgag ccggccagca
     2761 cacaatgctc caggatgtgg ggcaggcccg tggaatccgc gggcggcgtc ctgaagccga
     2821 tggcgtaaag gttctcccgg tccatggcgt gcagatgcag cactttggcg ccggttttgc
     2881 ggtgctccat ttcataggcg gtcaagcgca tcgattcgat gacttcgacg cgcaacacga
     2941 taaacccctg gacgacatcg ccgctgtgca gggtcggctg caatacatgg atgggtgtca
     3001 cgggacgctc cttgaaaaaa atttttaccg cggacaatcc tgaatcgtca cgcggcggtt
     3061 ttgcgcctgg ccttccggcg tgtcgttgtc ggcgatcggg cgcgattcgc ccgcgcccag
     3121 gatgatcagg cgatgttcgt cgatggcgta ttgttcgacc agatattgtt tgacgctttg
     3181 ggcccgccgc ctcgacagct ccaggttgaa ggcgtcggaa ccgacgctgt ccgtatgacc
     3241 ggtgatgacg agcgtgtttt cgggatgctt caccagatat tccataatcg tttgcacgct
     3301 gcttttcggg atggcgtcca cctgcgtgga attggtgtcg aaggcaacgc tgatgttgac
     3361 gcagcccggg cggttgaaat cgtcgtcgaa gccggccacg ggcagcgctt tccctctggg
     3421 gctggagagc acgggggttg cggcaaccat tttctccttg gcggcttccg tcaccacgac
     3481 gtccttcgtc tcgctgctga agtagatctc gccgatcagg ccttcggcgg gcgttctggt
     3541 tcgctgggtg ttgatgtgga tttgcaggcc ggtggtgacg ccgagcggtt cgcccccgtt
     3601 gatgtcgccg aaaagttttt tgtagtcttc atacagattg cggcgctccg tgtaccactg
     3661 gccgattttg tccgttttgt tcctcaaaac aatgtagcgc attttgtcgc cgccgatctg
     3721 tgtgctgcgt ccgctccatc ctttgggtgc ttcattgtcc cagatatagc ctacgaccgg
     3781 cgtgttcagc gcggccggaa atccggtttc tttgaaagca atatagacct ggagcgcctg
     3841 atcgtcggct gtttttttgc ggacgtcgcc gccgacgggc attttttcga ctttccaacg
     3901 ccaggaaaga atcggaaagt ctttcacgtt gacgcgcgcc gacgtgcgca cgccgaagga
     3961 cgagttgccc tgcgaccgca gatgcagata atagttgtcg ccttccttgg agattttcag
     4021 ggacggcgcg ccggtttttt tctccagcgc ccatccgggg ggcgctccgc ccggcagatc
     4081 cgcggaacgg aatttggtga cgggaatgac ggccgcttca acggcggcac agaagaacag
     4141 cagcgcggcc aacagcggga aaaaggggaa caaaaggctt cttttcatgt ccgctgctgt
     4201 cccgacagga tttcctcgcg cagcgccatg atttgcgtcc gcgtaaacac ggactcaacg
     4261 cagtctgcgc gctgaaggat gttttcccgc ccgccttcct cgcggtcaat cagcgtgatc
     4321 acccggtcca ccaccagacc ggcgcggcgg gcctgttcga tggcggtgat cgtcgagccg
     4381 cccgtggtga tgacatcgtc gatgatcgca accctttcgc cggccgcgac gttcccctcg
     4441 atggcgcttt tcgtgccgtg ggccttgaca tctttgcgca cgatgaacga tttgatcggt
     4501 ttgcccctct ggaaggaaat cacggccagg gcgttggcga tcgggtcggc gcccagcgtc
     4561 agtcctcccg ccgcggtgac cggcgcgtcc ttcagcatgt caaaaacaat ctcgccgatc
     4621 agattcatgc cctccgggtc cagcgtggtc ggtttgcagt tgaaataaaa attactctga
     4681 cggcccgacg ccagcgtaaa aggcgggttg tcgctgtatt tgaaagagcg ctccaggatg
     4741 atttttccaa ggcgcttttt agcggtgctt tgatccatgg acatcatatt ttcctccggt
     4801 ccggatgata ttacgagcgg gtattctcgt ggatgacaaa tttgatgggc agttccgccc
     4861 atgttttata aggaacgccg gactttttag ccggcacgaa tttccagtct ttgaccgcgg
     4921 caaccgcggt gtggtccaga atggcatagc cggatgactg ccggacgacg gctttgccca
     4981 cgcgtccgtc cgtcaggatt tcaaccgcga ccagcaccac gccctcgtaa cccctttgcc
     5041 gggccgtttc cggataaccg ggcggcgggt tctcgcggta tcgggggtag gcgttggaaa
     5101 cgaccggctg cgcaacgggg cccgcctgcg ctcttgcggc atcggatgca aacccgtggc
     5161 cgggcgcttc cgcatagacc gcctttgggg ttccgaagaa cgtggttgcg gcatcggctg
     5221 cggctttttc atcggccgaa acggctgtgg agaactccgg ctggaaattc tgcgcggtct
     5281 gcatttcccc tgtggcggaa gaccggccct ccgcggcgga aggcttcttt tgcccgaccc
     5341 gtggggcagg cgacgaagaa gcgtccttac cggaagcgat ggatgcccag actacatcaa
     5401 gtctgcccgt catctccggg accgttgccg ggccggacag gccgaaaatc aggacggccg
     5461 ccgccagggc gtggagtccg accgatgcgc caatgctcaa aaacctgttg aatgtttccg
     5521 tcgtcataac cattatttgt tgatcagttg ctccaccgct ttgacggcgg ctacgccgtc
     5581 tctggcgaag gatgcgccga tggattcggc atacgcctgc gtcaccacgg ccccgccgac
     5641 gagaaagtcc gtccggattc ccttagcgcg ggcaagcgaa atgatctcct tcatgtggac
     5701 catcgtcgtg gtcatcagcg cggaaagtcc gatcacgtcc ggcctttcct tttgggccgc
     5761 ctcgatgatt gcctgatcct gaacgtcttt gcccagatca ataacgcaat atccgtaatt
     5821 tctcaaaagc agcgcgacga tattcttgcc gatgtcgtga atatcgcccc ggacggttgc
     5881 cagcagaact ttgccttttt ccgcggccgc cttttgcttc agatacgggt ccagcagggc
     5941 cagggctttt ttcatcgttt ccgccgcggc catcaactgc ggcaggaaat aaatcttctt
     6001 ttcgtacagg ttccccaccc gcacaatggc cggaatcagc acctcatcga cgatccggga
     6061 ggcgggaagt ccggcctgga ggagcgcttc gacaaaagag acgatgcggt cccgatcgcc
     6121 gtcgagaaca gccgcggtga ctttttcctc cggggacatt tgcgccgaag cggtttgcgg
     6181 cgccgcaggt gcctgttcgg aaaaatggcg gatgaataag aggggcgcct tttcccgggc
     6241 gacaagagcg tctcccgctt ttttcacgtt catgaattcc acgccggccg gattggcaat
     6301 ggccatcgaa agcccgcagt actgcgccat ggccaaaaac gcggcgttga gccaggggcg
     6361 ccccggcatt ccgaaggaaa cattggacag gccgagaacc gttctgcttt taaaagtctt
     6421 tttgcaccag gccaccgtgg ccagcgtttc ccgggccgcg tctttgtcgg aggcaaccgc
     6481 catggtcagc ccgtccacaa taatatcctc tttggtcatc ccggccttct gcgcttttgc
     6541 ataaatcgag cggatgaccg cctggcgctt tggggccgtc ttcgggatgt tgccgtcggt
     6601 gaggggcagc aaaatgaaca ttgcgccgta tttcgcggcc agcggcagaa gcctggcgag
     6661 tttttccttt tcgccggaaa tggaattgat cagcatccgg cccggataga ggcgaagcgc
     6721 cgcctcgatg gtttccaccc tcgacgaatc gatgaccagc ggcaggcggg tggtcgcggc
     6781 gaggagcccg atcacttttt taatcgttgc aacttcgtca atgcccggca ctccgatgtt
     6841 gacgtccaaa agagatgctc cctggatttc ctgctcctgc gccatctggc ggatgatgga
     6901 cagtttgcct tccatcaact cctgctgcag ggccttcttg ccggtgggat tgatcctttc
     6961 gccgacgacc agcaggggct gatcctcgtc cagcgggaga tggcttctgg cggaggaaag
     7021 cgccgcgagc gatgtccggc ggggctttgc cggcttcctt tttccgacag ccccggccag
     7081 cgccgcgatg tgggcgggcg tcgtgccgca gcagcctccc agcaggttgg cgcccgctgc
     7141 gatcagcttt ttggcggaag cggcaaaagt tttggcatcc atgtcgaaaa cggtttttaa
     7201 tccctcgagc cgcggcagac cggcgttggg tttggccacc agcggcaccg tcgcataggg
     7261 cttcatggcg gcgatggatc gggccatttg ttcgggaccc gtggagcaat tgcagcctac
     7321 ggcatcggcg cccaggctct gcagggtaat gagcgccgct tccggcggcg ttccgcccag
     7381 tgtgtgcccg tcgttttcaa atgtcatgga agcgatggtg aaaatgtccg caagctcctt
     7441 taccgccagc agggccgcgc gcgcctcctg aatatcgatc atcgtttcga tcacgatcag
     7501 atcgcagccg ccgtcgatca ggccgcgcgc ctgttcttta aacgcatcga ccgcctcttc
     7561 aaaggccagc ggaccgaaag gctcgatgaa caagccggtg ggtccgatat cgccggccac
     7621 cagcgctttt ttgccgcagg ccagccgcgc caggcgcgcc agttcccggt tgaccgaaca
     7681 aacgtctttc ttcacgccga attgcttcag cttgaaacgg ttcgcgccga acgtgcaggt
     7741 gtaaaccact tgggcgccgg ctttggcata ggaggcgtga atatcctgaa tcaccgttgg
     7801 attttccaga caccagattt cgggacagac cccggcgggc aatcctcttt tctgcagctc
     7861 ggtgcccgtc gctccgtcca ggatcaagag ctgtttctgt ataagacttt tgattttccg
     7921 gcttttcgtc acgtcaactc cctgcgggac tcttttttag tgagccctta tatcatcttt
     7981 gcccttccag gtgggatttt tttcaacagc gccagccatt tctattggaa atcaagatgt
     8041 tatagtcaat tgtcgaaaaa ccggatggcg gcgggcggcc atccggacaa tggcgccgcc
     8101 catctgtcca tagctgatgc cggccgcctc cgcggcgcag gccatgctgg cgtcgtggct
     8161 gatgtccgca ttggggttga tgtccaggat gtaaaaaaca ccgtcccgca gccggacatc
     8221 catgcgggcg taatcccggc agcccagcgc ccggtaggca tcgcagcata cgttttccag
     8281 cgccgtcttt tcttcggggt cgagaggcgc gggcagcctt gtttcgattt tttcgtacgc
     8341 atcggagccc ggaatgaatt tggcatcatg gccgcagagg cggtcgcgga tgtctttgaa
     8401 aaatgcaaaa tccatttccg caacaggcag caccgtgagg atttcgttgc cccagaccga
     8461 cacgtgaaat tcgcgcccgt cgatgaaatc ttccaccaga gccggctgcc ggtaggtctc
     8521 cagaataaat ggaatgcgcc ccgccagttc caccgcatcg agcgcgacgg aatcacggct
     8581 gatgcctgcg gagcaatgtt cgttgaccgg cttgacgatt gcggggaacc tttcccaccg
     8641 gacggcttcg ccggcctgaa agatgcgcca ggcgggcgtg gggatgccgg ccgcgtcgag
     8701 cagagctttg acggcggcct tgtcctgcgc aagcgccagg gtttctgagc ccgcgccggt
     8761 gaaggtgaag cccatcgcct cgagccgctt ggctaccagc cactcgctgt gggaaattcc
     8821 cggcagactt tcacaccagt taaaaacgat gtgcgaggcg ggatcaaagc ggcccagaca
     8881 gccttcgaga tcgtcgtcgg caattgaagc aaaaaccgtc ggatagccgg actcgatgag
     8941 cgccaggccg agcttcgtgg actgaccgat cacttcttct ttttcggcat ccgtccagtc
     9001 cggatcgacg ttgtagagca aaacaacagg aattttgtca ggttttatgt ccaacgcctc
     9061 ggaagatttg agcatttctt tttcctttca ggggtgctca ttaaacatat ttcttttgga
     9121 attccaagaa tgaatttaca aatgtccggg ccggtgatat tttccaagcc gggaaagcgg
     9181 aaaggatgaa aaacacaaaa cccatggaat gcctgcgctg cggccgctgt tgcctggccg
     9241 atttcacggc ctacgcgacg gaggaagaca tccggcgatg gaaagccgaa ggaagggacg
     9301 atatcctgga ggtcctggaa agggggcagg cgttctggga gggggaccgt ctgatgtcga
     9361 gggagtcggg aaccccgctt gccggatgcc gtttttttgc tttcgacggc gagcggtact
     9421 tctgcgccat ccatgaaaca agacctgcgg tgtgccgcct ttacgagccg ggctcctccg
     9481 aaatctgccc gcagcattca aggttgggta agggaaatcc gcggaaaaga attcgcggtt
     9541 gacgcgggca ggatagaatt gcttttgtgc gactcttatt aaagattatt cttcgccaag
     9601 agattcaaga tccgccagat ctttctttcg acccattgcc cgtttattgg ctatatattc
     9661 ttttctgccc aggaagtgaa caggaatatc tccgtataaa ccttttacct tccccttatc
     9721 agccttttcc catgggactc cggttattga agtcatgatg tcaatgcgga ccggaggaac
     9781 gccaagctgt acaacagaat caggattttg aaaatcttca atggtcagat tcagggcgcc
     9841 aaaaccaaac tcagccaaag cacataagat tctctgtgcg ttctcggtcg aaggtttaat
     9901 caaaatatca atgtcgccgg taaaacgagg cgctccatgg tatgcaaggg cataagcccc
     9961 gacaatcata tattcaattt tatgcccgtt gaataactcg agcaactctt tgaagtcttt
    10021 ctgtgcttcc ataatattgc tttctcagat aatcgacggt tgcaagacgt tcttccacag
    10081 gccggcttaa ccagtacgcg agattgatcc tgtctgactg gaaatcatga agactgcttt
    10141 tacgaacgac ttttgtaatc atgattatca attagcataa aatcattcat atttctacta
    10201 tcgcttgccg aacgagccgc atcaccggcg cgacagtccc cggtccgact tgagcggatt
    10261 gtcaggcaag tcatcacgtg atccgcaaag caggaatagt gcggtttcgg gaacaaacag
    10321 gtgtctcctt tggggctcaa cgaaaagctc gcccgcagca acgcctagcg ctgcgatcgg
    10381 gtggtgcgat gggttacgtg gtattattgc tccttcatta caatatgctt aacgattaat
    10441 tgtgtatgat ttggcttttt ccgatttgtg ccttccatcc taattcatct gcagcaacaa
    10501 gaaaagcgaa atatcctatt aaatccgtct tcttcaaaag cagcgtttgg catatcagaa
    10561 caataagcaa caaaaaaccc gctgtgcatg cgggtttttg catctctttg gatgatgttg
    10621 gataatgacc tggtgggatg aggaggatcg agctcaacac gtaagtcatc gtaaataaat
    10681 aatgttcacg gtaactattt tttgacatac ccccaaaagt gaccccaaaa taacgcctat
    10741 atcatgcatg tgtcacagaa acggccttct tcaggacgtc ttttacaaaa gcattaagtg
    10801 tcttcccttc gcttaaagcg ccggcaacga gcagttcatg aagggacgga tcaaggcgca
    10861 ggttgaaggc gcctttgaaa ggcttctccg gctcttttcc gattttccgg caggtttcga
    10921 aatagttttc aatggccgcc ttgaagtcct gttcaagctc ttccaccgac gaaccttcaa
    10981 aactgattgc atcattgatg cccaggacgt gcccgtgaaa tatccgatca tcggcgtcaa
    11041 actgcacgct cccgacatat ttcttgtatt ttaaataatt tttgctcatg gcttcacccc
    11101 tatgacggcc aggaaatccc ttattttttc gactgcgtat tttttcattt ccccgctcgg
    11161 atgcggcttg tggatgtcca gcacccgctg atcgaaaacc acagaaacac gcgatcccct
    11221 gccttcaaaa atatcggcac cgattgcctt gaatagtgat tcgaccgcgc gccaggaaac
    11281 gtcgcagcgt gtcggctttt cataaatatc ggcaagggtc ttccgctgtt ttttgttcac
    11341 atacgaaagc gtaccagaag ggaaaagaaa aggcaaagaa aaaaccagga aacggttaca
    11401 aatcattgct cccctctcca gggagatctg cacacaaaaa acccgctgta catgcgggtt
    11461 tttgcatttc tttggattac gttggatgag gtcttggtgg agatgagggg gatcgaaccc
    11521 ctggcctcgg cgttgcgaac gccgcgctct cccagctgag ctacatcccc ggcctggcaa
    11581 aaaagcgtgc ggagcatatc cataagtttg cgcaagtgtc aagaattagt tgcgttgagg
    11641 aggggtcggc tgatgcgccg acccttccct tactttgcgt tgctgctggt cggcgctgtg
    11701 gcgcccggac cccgtcgctg caggccgttt cgttttccaa acttttcttg acaaaaccaa
    11761 ggccttttcc ctatactcgc agccgtgccg gagagtatcg gaagcgtcgt acagacggga
    11821 aataagccgt ttgacagaac agttttgacc atcaaaccat aggacaaaat gagagctttg
    11881 aataaaggcc atttttgtca tttcgaccga agggagaaat ctt
//
LOCUS       DAOSRM010000069        11818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5400_length_11818_cov_113.241860, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000069 DAOSRM010000000
VERSION     DAOSRM010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11818
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5400_length_11818_cov_113.241860"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLA58_07400"
     CDS             complement(<1..557)
                     /locus_tag="PLA58_07400"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOR62656.1"
                     /translation="MDPFGIALILFVSALFSGGLAAYAWRRRGTPGTGIVFFLMLGTT
                     IAVFAYGMELLSTTLADKMIWVRIRYTGTALIYPMYLLFALWHTNKQKWLAAGRVALL
                     LVLPVFSVIGLFTNSLHHLHYTWVGLDTNGPFPMIVKTYGPLYGFYVITSFGYVLLAL
                     AILGSNYLLTPSFFRRQTAALMIGTG"
     gene            810..1109
                     /locus_tag="PLA58_07405"
     CDS             810..1109
                     /locus_tag="PLA58_07405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOR62657.1"
                     /translation="MITVIAKLPVKEGKMDEAMAAFSVLISKVASEEGTLLYSLNRDK
                     ANPDVLVVVEQYRDKAALEFHSSTPHFKEFFSASAAFIGGRPEMTVMKEVQRAKP"
     gene            complement(1161..1637)
                     /locus_tag="PLA58_07410"
     CDS             complement(1161..1637)
                     /locus_tag="PLA58_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HOR62658.1"
                     /translation="MKQITHQMAEEWIAAAVRSPRLRTNYNIHESLSDPIQRLFVAAG
                     RGSYFRPHLHLGKSEFAMVLRGCFDVFTFDAHGLVAGRVCIGPAEETFALEIPADTFH
                     TWIPLAEVSVFFEVKKGPYDPATSMVFAPWAPEEGSPDTAVFQSKLLAARAGDRIA"
     gene            complement(1667..2458)
                     /locus_tag="PLA58_07415"
     CDS             complement(1667..2458)
                     /locus_tag="PLA58_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR62659.1"
                     /translation="MSLTRFSRLAVNFCITLGLWAYFTLGYLLLMFVLFIPCYLRLKA
                     GDAVLQKLFHVHLKIFFVLVDFMSPGVRFAVSEDVRRLRSSIIVCNHLSYLDPILLVS
                     LFPRQTTIVKNTFFSVPIFGWFLRKAGYVPSSPTEMYGPAMINHLESIKRQLAAGGNL
                     FVFPEGTRGREGRLAPFNRGVFSIARYCNTGLKLVLIRNTDRLFRPGSFSFDTRGEFT
                     VSMEWIGSLTENDLKNSSISAAADQARTLFERKIRETQTGGDAQE"
     gene            complement(2465..3706)
                     /locus_tag="PLA58_07420"
     CDS             complement(2465..3706)
                     /locus_tag="PLA58_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOR62660.1"
                     /translation="MTERRVVITAASAITPIGHGQKPIVESLVNGKSGVATLRDDHFV
                     SRFIHSRVYGTVGYPIEYDFSRQHRKTMGPVSYYACQVAKEILEQAGLPRNFVTGGRL
                     GIAFGSIHGSPTVQREIYRIFFNAGDRESYQGIGAVDYLKSMVHTTAVNISKMFGITG
                     RIISSGTACTTSSQSIGYGYEAVKYGLQDAMLCGGADEYDTTTVAVFDNLLACSTAFN
                     DRPEQTPRPFDRARDGLVVAEGAGAVMLEEYEFARKRGAEILAEVIGFACNSNGGDLI
                     LPNLDGITKTLKLGLENARIHPSDVDLISAHATATKMGDIIEAQATGAVFGKTPAVVA
                     LKSYMGHTMAACGAIETVVTLYMMQEGFIAPTLHLEEIDDRCAMIRHVQKPEETPVKT
                     AAIQNFAFGGVNTSLLIRKLT"
     gene            complement(3707..3979)
                     /locus_tag="PLA58_07425"
     CDS             complement(3707..3979)
                     /locus_tag="PLA58_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOR62661.1"
                     /translation="MTRQEIEKEIRAIFLREFEVENPEPDVNLRDAYGFDSIDAIELL
                     MEIEKVLGTELTQAEKKKAMDIRTLNQIIDYVQILADCRQTESEKN"
     gene            complement(4051..5523)
                     /locus_tag="PLA58_07430"
     CDS             complement(4051..5523)
                     /locus_tag="PLA58_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR62662.1"
                     /translation="MQKPAEGKNTVVIGSGAGGLSAGILLALLGFRVTIVEKNEEPGG
                     LMRSYSRGGIDCPVGVHYVGALGASEPLGKMFRVLGIDPESLFSRMENRAVTDRYIFD
                     DFVFDLPEGLDAYEKNLREACPADRAALDALINGLRATAGVMAASPFFFDGGSPFSNM
                     DSFRPMGELLDCLGASPKLRAVLAAPCQLIGVALEDCPVIFHQMVTAGYLFSSWRPKS
                     GRRLADAFAARFEELGGRLLPGFTVRHVRTRQGRVTGVDLEEGDFLPADAVVAAIHPK
                     VLLGLLDQDALKASLRERISGLEETNGVLAVQAGVDAQAHPAVDYNLYRLRVNDRGEI
                     ENGFFYQVLRGDGRETNLLSIITRSHYRDWRRWENTATGRRGETYEREKKALADKLVA
                     ESSAVFGPLKNLRLLDVFTPLSLRDFVNCPEGSCYGVLRSSRQLLKIASLGKLPVGGL
                     YLAGQNALAPGLMGCLLGSFAVARKMAGHEHFGKELERLL"
     gene            complement(5541..6194)
                     /locus_tag="PLA58_07435"
     CDS             complement(5541..6194)
                     /locus_tag="PLA58_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62663.1"
                     /translation="MNVRFFLILSLLLASCSSLPEIRPSADAGSACPSPFVVQKTRFI
                     HTIEAAAAGETRSVMIGVTVADPASRTLSCALMSPEGIVFFEASRTPSGTNISRALPP
                     FDAAEVARGMLDDIELIFLKPTEEINRAGVGDSGRTVCRRRLRDGGWLDVGKDRDGRI
                     RIQRYSEGGRLRRSVALAAGVNPYAAIELQASEWTGYSLSMTLIEAETVPDEIRSGK"
     gene            complement(6191..6814)
                     /locus_tag="PLA58_07440"
     CDS             complement(6191..6814)
                     /locus_tag="PLA58_07440"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOR62664.1"
                     /translation="MRAKAAGPLSVLLLVLLMALPAAGADDFDRLRRDASRISSLSAD
                     FVQKKSMKILSKPLVSTGKFFYAAPDSIRWEYVKPIRSIVISEKGTSKRYIASGGKMI
                     EDKTGGVQAMKIVLDEVAGWMKGKFSANPSFAASVREGPETVIVLTPTGKNTAGMIEK
                     IEITVSRKDAVVKTVRIVEGAAAETRIDFLRTAVNPALPPGTFQDIP"
     gene            complement(6817..7551)
                     /gene="fabG"
                     /locus_tag="PLA58_07445"
     CDS             complement(6817..7551)
                     /gene="fabG"
                     /locus_tag="PLA58_07445"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOR62665.1"
                     /translation="MEERKIALVTGASRGIGRATAVELARTGYFVIINYNASEAAAAE
                     TLAQVQAAGGSGETRKFDVADAAGVKEALAAIAQEHKRIDALVNNAGVTADGLFMMMG
                     EEEWLRVINTTLNGFYFVTKPVLREMVRKKRGCIVSVSSISSMLPNRGQANYAAAKAG
                     LNAATRSLAKEVARFGIRVNAVAPGPIATDMLSSMPMDENIIKSVIPMARVGKPEEVA
                     RVIRFLCSEDASYITGQVISVNGGMF"
     gene            complement(7579..8001)
                     /locus_tag="PLA58_07450"
     CDS             complement(7579..8001)
                     /locus_tag="PLA58_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62666.1"
                     /translation="MIDIESVIPHREPIKIITQAVELQDDSGIAAAVVNERWPLFDGR
                     TVHSIALIEAVAQTAALVEGYKRKKKGQDAIKGWMVGIKSAEFHRDVLDLQTCITVSI
                     RRQCSFDDYAVINGTVTSGNETLLTAVLQAIRLNKDEG"
     gene            8157..9548
                     /locus_tag="PLA58_07455"
     CDS             8157..9548
                     /locus_tag="PLA58_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOR62667.1"
                     /translation="MSAEQKDPFVVAYRKLLSPSKSLCRKEFTCLGDTYGEVFSMAAG
                     LKKTLSGRGVETSVCLCTENKAVVAASILAALAGVCNLILPHSFSTHALLETYEATGF
                     DAAVADRPEEMPGVEMISPTAGDMADLNPGDLRDPDRSFLKLFTGGSTGKPRVWSKTP
                     RNLLAEAFYLRDRFGLTGDDVFLSTVPPYHIYGLLFSILLPFVSRARVLPEIYTFPQE
                     ILSTINRHRATVLVSVPISYRSLKVDNLSVPSLRLAFSSSGPLDRSDARYFLKKTGLG
                     IHEIYGSTETGGVASRSVSENTESWQPAGPVSWRMFGRRLAVRSDFVSSEMERDAEGF
                     CVTGDEVQPDRDGRFLLLGRADGIVKVAGKRVDLKEVQNKIQTLPTVRDAVVIALPAE
                     KGRESEIAALVACDLTEIQVKKMILEKLEPYAMPRRVHIVPSIRRTATGKIDYRRVEQ
                     IFLRAKEKSSQQK"
     gene            9949..11439
                     /locus_tag="PLA58_07460"
     CDS             9949..11439
                     /locus_tag="PLA58_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOR62668.1"
                     /translation="MTRFREYDKYDGLGLAELVGKKEVTPLELCEEAIGRIDELNPKL
                     NAVICRMDDAARAAAKGAIPQGPFSGVPFLCKDLVSAIAGVPMTKGSKSCRNYVPSFD
                     SEMARRFRAAGLIVLGKTNTPEFGLMGITEPELHGPTRNPWNPDHTPGGSSGGSAASV
                     AAGFTPLASGGDGGGSIRIPSSCCGLFGLKPTRGRNPLGPESGEVWQGAEVEGVLTRS
                     VRDAAAVLDATNGADPGAPYILPRPERPYLEDAGQDPPRLKVGFTVESPLKADVHPEC
                     VEAVKNAAKLLESLGHAVEEAGPDIDGEAVGKSYIMLYFGEVAADIRELTSVVGRPLT
                     HADTEEATWALNLLGNTFSAGDFVLAKRQWNTFARRMAKFHETYDLYLTPTIASLPPR
                     IGELQQKPAERAFLKIINSLNLGGLLKATGIADMLATKGLSKMPFTQLANLTGQPAMS
                     VPLHWSREGLPCGVQFIGRFGEESLLFQLAGELERAQPWFDRRPSL"
BASE COUNT         2415 a         3433 c         3524 g         2446 t
ORIGIN      
        1 cccgtcccga tcatcagcgc ggccgtctgc cgccgaaaaa aggagggggt gagtaaatag
       61 tttgatccca ggatggccag cgccagcagc acataaccaa acgaggtgat gacgtaaaac
      121 ccgtacaggg gtccgtacgt tttgacgatc atgggaaagg ggccattcgt atccagtccg
      181 acccaggtat agtgcaggtg atggagggaa ttggtaaaga gcccgatcac ggagaagacc
      241 ggcaggacca gcaacagggc gacgcggccg gctgcaagcc atttctgctt gttggtgtgc
      301 cacagcgcaa acagcagata catcggataa atcagggcgg ttcccgtgta gcggattctc
      361 acccagatca ttttgtcggc aagggtggtg gagagaagtt ccattccgta ggcgaatacc
      421 gcgatcgttg tgcccagcat taaaaaaaag acaatgccgg ttcccggtgt gccgcggcga
      481 cgccaggcat aggccgcaag gccgccggag aaaagggcgg aaacaaatag gatcaatgca
      541 attccaaagg gatccatgaa agcgaatttc cttacttcac tcccggtcgc cggtttttgc
      601 gaccggccgg cgcgttggta aaccgtatga ctattaaaac cgtttgggca aatgcaatcg
      661 cgatttgata gtattcattg catattttat ctgttttttc aatgatttcc tggttgttat
      721 cccgaagcgc ttgctacttg gggtgtgttt atgctatggg ggcacatcta gtcggaaaag
      781 cttcaaaaaa ccatccggga ggtgtttcga tgattaccgt tattgccaag ttgccggtga
      841 aagaggggaa gatggatgaa gccatggccg ccttctcggt gttgatctcc aaagtcgctt
      901 cggaagaggg gacgcttctg tattcgctca accgggataa agccaatccc gacgtgctgg
      961 tcgtggtcga acagtacagg gacaaagccg ccctggagtt tcattcctcc acgccgcact
     1021 tcaaggaatt tttctcggcc agcgccgctt ttatcggcgg ccggccggaa atgacggtga
     1081 tgaaggaagt ccagcgggcg aagccgtagg aaacggcctg tcacccgcag gccggtcgcg
     1141 accgtttcct tttcgatctt tcacgcgatg cggtcgccgg cgcgcgccgc cagaagtttt
     1201 gactggaaaa ctgccgtgtc cggagagcct tcttcgggcg cccagggcgc gaagaccatc
     1261 gaggttgccg gatcataagg gcccttcttg acttcgaaga agacggagac ttccgccagg
     1321 ggaatccagg tgtggaaggt gtcggccggg atttccagcg cgaacgtttc ttcggccggg
     1381 ccgatgcaga cccgcccggc aaccagacca tgagcgtcaa acgtaaagac gtcgaaacaa
     1441 ccgcggagca ccatagcgaa ctcgcttttt cccaagtgca gatgcgggcg aaaataggat
     1501 ccgcgccccg cggcgacaaa cagccgctgg atcggatcgg acagggattc gtggatgttg
     1561 tagttggtcc gaagccgcgg gctgcggacc gccgccgcga tccattcctc cgccatttgg
     1621 tgggtgattt gtttcatgct tcctccccga tgcccctaca aggggtttat tcctgcgcgt
     1681 cgccgccggt ttgtgtctct ctgatttttc gttcgaaaag cgttcgggcc tgatcggccg
     1741 ccgcactgat ggaagaattt ttcaggtcat tctccgtcag cgatccgatc cattccatcg
     1801 aaaccgtaaa ttcccccctt gtatcaaacg agaatgagcc ggggcgaaac agccggtcgg
     1861 tgttgcggat caacaccaat tttaaacccg tattgcagta cctggcgatg ctgaacacgc
     1921 cgcggttgaa gggcgcaagc ctgccttcgc ggccgcgtgt gccttccgga aagacaaaca
     1981 gattgcctcc cgccgccagt tggcgcttga tgctctccag gtggttgatc atcgccggtc
     2041 cgtacatttc ggtgggggag gagggcacat aacccgcctt gcgcaaaaac cagccgaaga
     2101 tggggacgct gaaaaaagta tttttgacaa tggtggtttg ccgcgggaag agggacacca
     2161 gcaggatggg gtccagatag gaaaggtggt tgcagacgat gatggaggag cggagcctcc
     2221 ggacgtcttc ggaaacggcg aaccggacgc cgggggacat gaagtccacc aggacgaaaa
     2281 aaatcttcag atggacgtgg aataatttct gcaaaacagc gtcgccggct ttcagcctca
     2341 gatagcaggg gatgaacaaa acgaacatca acaggagata acccagtgta aaataggccc
     2401 agagccccaa tgtgatgcag aaattgacgg cgagccggga aaatcgggtg agggacatct
     2461 ttcgtcaggt caactttcgg atcagcaggc tggtgttgac gccgccgaag gcaaaattct
     2521 gaatggccgc agtcttgacc ggagtttcct ccggcttttg aacatggcgg atcatcgcgc
     2581 agcggtcgtc gatttcttcc agatggagcg tcggggcgat gaagccctcc tgcatcatat
     2641 acagcgttac aaccgtttcg atcgcgccgc aggccgccat ggtgtgcccc atatagcttt
     2701 tgagggccac tacggccgga gtcttgccga agaccgcgcc cgtggcctgc gcctcgatga
     2761 tgtcgcccat tttggtggcc gtggcgtgcg cgctgatcag atcgacgtcg gacggatgaa
     2821 tccgggcatt ttcgagaccc agtttcaggg ttttggtgat gccgtccaga ttgggaagga
     2881 tcaggtcgcc gccgttggag ttgcaggcaa aaccgatgac ctccgccaga atctccgcgc
     2941 cccgctttcg ggcaaattca tactcctcca gcatcacggc gccggcgccc tcggcgacca
     3001 ccagaccgtc gcgcgcgcgg tcaaaggggc ggggcgtctg ttcgggccgg tcgttgaagg
     3061 cggtcgagca ggcgagcagg ttgtcgaaaa ccgcgacagt ggtggtgtcg tattcgtccg
     3121 cgccgccgca gagcatcgcg tcctgcaggc cgtatttgac ggcttcgtat ccgtagccga
     3181 tggactggct ggacgtagtg caggccgttc cggaggaaat gatccggccg gtgatgccga
     3241 acatcttgga aatgttgacg gcggttgtgt gcaccatgga tttgagatag tccaccgcgc
     3301 cgattccctg atagctttcg cggtcgccgg cgttgaagaa aatcctgtaa atttcccgct
     3361 gcacggtggg cgatccgtga atcgatccga aggcaatgcc cagccggccg cccgttacaa
     3421 aatttcgcgg caggccggcc tgctccagaa tttctttggc gacctgacac gcatagtagg
     3481 acacgggccc catggttttg cggtgctgcc ggctgaaatc gtattcgatc ggatagccga
     3541 cggttccgta aacgcgggaa tgaataaaac ggctgacgaa atggtcatcg cgcagggttg
     3601 ccactccgga tttcccgttt acaaggcttt cgacgatggg cttttggcca tgcccgatgg
     3661 gggtgatggc cgacgccgcc gtgatgacca ctcgacgctc ggtcatttag tttttctccg
     3721 attcggtttg ccggcaatcg gccagtattt gtacatagtc aataatctgg ttcagcgtcc
     3781 ggatgtccat ggctttcttt ttttccgcct gcgtcagctc cgtccccagg accttttcaa
     3841 tttccatgag cagctcgatg gcgtcgatgc tgtcaaaacc gtaggcatcc cgcagattca
     3901 cgtccggttc ggggttttcc acctcgaatt ccctcaagaa gatggcgcgg atttcctttt
     3961 caatttcctg gcgtgtcatg atctgcagat gtctccttta gcggcaaaag tgcccggaaa
     4021 gctaacataa ttcagcgaag tcgtccagca ttagagtaac cgctcgagtt ccttgccgaa
     4081 atgctcatgc cccgccatct tccgcgccac ggcgaaggac cccaggaggc accccatcag
     4141 ccccggggcc agagcgtttt gtccggccag ataaagcccg ccgacgggaa gtttgccaag
     4201 cgatgcgatt ttgagcaact gccgggaaga gcgcaggacg ccgtagcagg aaccttccgg
     4261 gcagttcacg aagtcgcgca gcgaaagcgg cgtgaagaca tccagaagac ggaggttttt
     4321 cagggggccg aagacggcgg acgattcggc aacgagtttg tccgccagcg cctttttttc
     4381 ccgttcgtag gtctcgccgc gccgccccgt cgcggtgttt tcccaccggc gccagtcgcg
     4441 ataatgcgag cgggtgatga tggacagcag atttgtttcc cgtccgtcgc cccggagcac
     4501 ctgataaaaa aagccgtttt caatttctcc gcggtcgttg acccggagcc ggtaaagatt
     4561 gtaatcgacg gcaggatggg cctgcgcatc cacgccggcc tgtacggcca ggacgccgtt
     4621 ggtttcctcc agaccggaaa ttctctcccg cagggaggcc ttcagagcgt cctggtccag
     4681 cagtccgagc aggaccttgg gatgaatggc cgccaccacc gcgtccgcgg gcaggaaatc
     4741 gccctcttcc agatcgaccc cggttacgcg tccctgccgg gtgcggacat gccggacggt
     4801 aaagcccggc agcaaccgtc cgcccagctc ctcaaaccgc gccgcgaagg cgtccgccag
     4861 acgccgcccg cttttcggac gccaggatga aaacaaatat ccggccgtga ccatctgatg
     4921 aaaaatgacc ggacaatcct cgagcgcgac gccgatcagc tggcagggcg ccgccagaac
     4981 cgccctcagt tttggggacg ctcccaggca gtcgagcagt tctcccatcg gacggaagga
     5041 atccatgttg gaaaaggggc tgccgccgtc aaagaagaaa ggcgatgccg ccatgacgcc
     5101 tgcggttgcg cgcagcccgt ttatcagcgc gtccagcgcg gcgcggtccg cgggacaggc
     5161 ttcccggaga tttttttcgt aagcgtccag gccctcgggc agatcaaaga caaaatcatc
     5221 aaaaatatag cggtcggtga cggccctgtt ttccatgcgg gaaaacaggc tttccgggtc
     5281 aatgcccagc acgcggaaca ttttgcccag gggttcggaa gcccccagcg cgccgacata
     5341 gtgcacaccc accggacagt cgattccccc gcgtgaatac gagcgcatca ggccgcccgg
     5401 ttcttcgttt ttttccacaa tcgtcacgcg aaagcccaga agcgccagca gaatgcccgc
     5461 ggaaaggccg ccggcgcccg agccgatgac aacggtattt ttcccttccg ccggtttttg
     5521 catataggtt ttcctgaaaa ttattttccc gaacgaattt cgtccggaac ggtttccgct
     5581 tcgatgagcg tcatggacag cgagtatccg gtccattccg acgcctgaag ctcaatggcg
     5641 gcgtagggat tgacgcctgc cgcgagcgcc acgctgcgcc ggagcctgcc gccttccgag
     5701 tagcgttgaa tccggattcg tccgtcgcgg tcctttccga catccagcca ccctccatcc
     5761 cgcaggcggc gccggcagac ggtccggccc gaatccccaa cgcccgcccg gttgatctct
     5821 tccgtcggct tcaaaaaaat cagttcgatg tcgtccagca tgccgcgggc cacctccgcc
     5881 gcgtcaaacg gcgggagcgc ccggctgatg tttgtgccgg agggggttcg cgaagcttca
     5941 aaaaaaacta tgccctccgg gctcatcaaa gcgcaggaaa gcgtcctcga tgccgggtcc
     6001 gccaccgtca cgccgatcat cacggaccgc gtttcgccgg ccgcggccgc ttcaatcgta
     6061 tggatgaagc gggttttttg gacgacgaaa ggggaagggc aggcgctccc ggcatcggcg
     6121 gacggccgga tttccggcag agacgaacag gatgccagca gcagggagag aatcagaaaa
     6181 aagcggacgt tcatggaatg tcctgaaaag tgccgggagg cagggccgga ttgacggccg
     6241 tgcgcagaaa gtcaatcctg gtttcggccg cggcgccctc cacgatccgc acggttttta
     6301 cgacggcgtc tttccgggag acggtgattt ctattttttc aatcatgccg gccgtgtttt
     6361 ttcccgtcgg tgtgaggaca atcaccgttt ccggcccttc ccgcacggag gcggcaaagg
     6421 acgggttggc ggaaaatttt cctttcatcc accccgccac ttcgtcgagc acgattttca
     6481 tcgcctggac gccgccggtc ttgtcctcga tcattttgcc gccggaggcg atgtagcgct
     6541 tcgaggttcc tttttccgag atcacgatgc tccggatcgg cttgacgtat tcccagcgga
     6601 tcgaatccgg ggcggcataa aagaatttcc ccgtcgagac cagaggtttg gacaggatct
     6661 tcatggattt cttctgcacg aaatcggcgg aaagagacga aatgcgcgat gcgtctctgc
     6721 gcagccggtc gaagtcatcg gccccggcgg cgggaagcgc catcagaagc acaaggagca
     6781 aaacggatag ggggccggcg gctttcgcgc gcatgatcag aacattcctc cgttgacgga
     6841 tatgacctgc ccggtgatgt aggaagcgtc ctcggagcac aaaaagcgga tcacgcgggc
     6901 gacttcttcc ggttttccca cccgggccat cggaatgacg cttttgatga tattttcgtc
     6961 catcggcatg ctgctgagca tgtcggtggc gatggggccg ggcgcgaccg cgttcacgcg
     7021 gatgccgaag cgcgcgactt ccttggcgag cgaacgggtt gcggcgttga ggccggcttt
     7081 cgccgcggcg tagttggcct gcccgcggtt ggggagcatg gaggaaatgg aagaaacgga
     7141 cacgatgcag cctcgttttt tccggaccat ttcacgcagg acgggttttg tgacgaagta
     7201 aaatccgttg agcgtcgtat tgatcacgcg aagccactcc tcttctccca tcatcatgaa
     7261 aaggccgtcc gcggtgacgc cggcgttgtt gacgagcgcg tcaatccgtt tgtgctcctg
     7321 cgcaatcgcg gccagggctt ctttgacccc ggcggcgtcg gccacgtcga atttccgcgt
     7381 ttcgccggag ccgcccgcgg cctgtacctg cgccagcgtt tcggccgccg cggcttcgct
     7441 ggcgttgtag ttgatgatca caaaataacc ggttcgggcc agttccacgg ccgtggcgcg
     7501 tccaatgccc cggctggcgc ccgtcaccag cgcgattttt ctttcttcca tgatggtatt
     7561 tttctccttt ggcgaatgtt atccctcatc cttgttgagg cggatggcct gaagaacggc
     7621 cgtgagcagc gtttcgtttc ccgaggttac cgtgccgttg atgacggcgt aatcgtcaaa
     7681 ggaacactgc ctcctgatcg aaaccgtgat gcaggtttgc aggtcaagaa cgtcccggtg
     7741 aaattccgcg gacttgatgc ccaccatcca gcctttgatg gcgtcctggc ctttcttctt
     7801 ccgtttgtag ccttccacca gcgcggcggt ctgggcgacg gcttcgatca gggcgatgga
     7861 atggacggtc cgtccgtcga aaaggggcca gcgctcgttg accacggcgg ccgcaatgcc
     7921 ggaatcatcc tgcaattcta cggcttgtgt gatgattttg atcggttcac gatgcggaat
     7981 cacggattcg atgtcgatca tcgccgctcc tccccttttt agtttaaatt aaagagaaaa
     8041 ccatccgaaa gtcaatcaaa aacattgcgt gccgccgccc tgtttcgcgc aaaacgctcg
     8101 ttgcttttca gccttttttc ttatagaaac ttcaaaaatg atttgtgcag gttcccatgt
     8161 ccgcagaaca gaaagatcct tttgtcgttg cctaccggaa gctcctctct ccgtcaaagt
     8221 cgctgtgccg gaaggaattc acctgtttag gcgacaccta cggcgaagtt ttttccatgg
     8281 cggccggttt aaagaaaacg ctttccggac gcggcgtgga gacgtccgtc tgtctgtgca
     8341 cggaaaacaa agccgtggtc gccgcctcga tcctggccgc tctggccggg gtctgcaatt
     8401 tgatcctgcc ccactccttt tcgacgcacg cgctcctgga aacgtacgag gcgacaggct
     8461 ttgacgcagc cgtcgcggat cgcccggaag aaatgccggg cgtggaaatg atttccccga
     8521 cggcgggcga catggccgat ctcaatcccg gggatctgcg cgatcccgac cggtcttttc
     8581 tgaagctttt tacaggcggc tccaccggca aacccagggt ctggtccaaa accccgcgca
     8641 atctgctggc cgaagctttt tatctgcgcg accggttcgg tctgaccggc gacgacgtgt
     8701 tcctgtccac cgttcccccc taccatattt acggtctttt gttttccatc ctgctgccgt
     8761 ttgtttcgcg cgcccgcgtg ctgccggaaa tctacacctt cccgcaggaa atcctctcga
     8821 cgatcaaccg tcacagggcc accgttctgg tcagcgtccc gatttcctac cggtcgttaa
     8881 aggtggacaa cctgtcggtt ccctcactga gactcgcttt ctcgtcctcc ggccctctgg
     8941 accggtccga cgcgcgctat ttcctgaaaa aaacaggcct gggcattcac gagatctacg
     9001 gttccaccga aacgggcggc gtcgcgtcgc gcagcgtgag cgaaaacacg gaaagctggc
     9061 agcccgccgg tccggtttcc tggcggatgt tcggccggcg gctggccgtg cgctccgatt
     9121 tcgtgtcgag cgagatggaa agggatgccg aggggttttg cgtgacgggc gatgaagtcc
     9181 agccggaccg ggacggccgg ttcctgctgt tggggcgggc cgacggcatc gtcaaggtcg
     9241 ccggaaagcg cgtggacctg aaggaggtcc agaacaaaat ccagacgctg ccgacggtgc
     9301 gggatgcggt ggtcatcgcg ctcccggccg aaaagggccg cgaaagcgaa attgccgcgc
     9361 tggtcgcctg tgatctgacg gaaatccagg tcaaaaagat gattctggaa aagctggagc
     9421 cgtacgccat gcccaggcgc gttcacattg ttccttctat tcggcggacc gccaccggca
     9481 agatcgatta tcgccgcgtc gagcagatct ttctccgggc aaaagaaaaa agctcacagc
     9541 agaagtagtg gatggtgaat agtgaatggt gaattgtaaa aagcgacgag tgtaaattgc
     9601 aaagaggaaa aactcctcgc tttcgactgg cccaaggtct cttgcttgtc gcccataact
     9661 cacattgccc attaccccgg atcccgtagg gaacggcctt gtgacttcgc gccacctcgc
     9721 gccaccttta ggtggttagg ttatcgtgac cgttccctac catctaccat tcactatcca
     9781 ctatccacca tcgtcacccc ctgcggaaaa gccttgactt caccggcgaa cggtcattat
     9841 acttcccgac aattcaggat tggggcattt acggcggcgt caggtcatca cttcaaccac
     9901 acgaacaacc ttttgttttt ttctgatcag tgaagaggga ggaaaaaaat gacgcgtttc
     9961 cgtgagtacg acaaatacga tggcctgggg ctggccgaac tggtcggaaa aaaagaagtg
    10021 actccgctgg agctgtgcga agaagccatc gggcgcatcg acgaactcaa cccgaagctc
    10081 aacgcggtca tttgccggat ggatgacgcg gcgcgcgccg cggccaaagg cgcgattccg
    10141 caaggccctt tttccggcgt gccgtttttg tgcaaagatc tggtttccgc catcgcgggc
    10201 gtacccatga ccaagggcag caaatcctgc cgcaattacg tgccttcgtt cgacagcgaa
    10261 atggcccggc gcttcagggc ggcggggctg atcgttctgg gaaaaaccaa cacgccggaa
    10321 ttcggcctga tgggaatcac cgaacccgag ctgcacggtc cgacgcgcaa cccctggaac
    10381 ccggatcaca cgcccggcgg ctccagcggc ggttcggcgg cgtcggtggc cgcgggcttc
    10441 accccgctgg cttcaggcgg cgacggaggc ggatcgatcc gcatcccctc ctcgtgttgc
    10501 ggcctcttcg gcctcaagcc cacccgcggc cgcaatccgc tggggcccga atcaggcgaa
    10561 gtctggcagg gcgcggaggt ggagggcgtg ctgacccgct ccgtgcgcga tgcggccgct
    10621 gttctggacg ccacaaacgg cgcggatccc ggcgccccct atatcctgcc ccggccggaa
    10681 aggccgtatc tggaagacgc cgggcaggat ccgccccggt tgaaagtcgg gttcacggtg
    10741 gaatcgccgc tgaaagccga cgttcatccc gaatgtgttg aggccgtgaa aaacgcagcc
    10801 aaacttctgg agagtctcgg ccatgcggtg gaagaagccg gaccggatat tgacggagag
    10861 gcggtgggca aaagttacat catgctgtat ttcggcgagg tcgcggcgga catccgggag
    10921 ctcacaagcg tcgtcggccg gccgctcacc catgccgaca cggaggaagc cacctgggct
    10981 ctgaacctgc tggggaatac tttttccgcg ggcgacttcg ttctggccaa aagacagtgg
    11041 aatacttttg cccggcggat ggccaaattt catgaaacct acgatctgta tctgaccccg
    11101 acgattgcct cgctgccccc gcgcatcggc gagctgcagc agaagccggc cgaacgggcc
    11161 tttctgaaaa tcatcaacag cttaaacctg ggaggcctgc tcaaagcgac gggcatcgcc
    11221 gatatgctgg cgaccaaagg cctgtccaaa atgcccttca cgcaactggc caacctcacc
    11281 ggacagccgg ccatgtcggt gccccttcac tggagccggg agggactgcc ctgcggtgtg
    11341 cagttcatcg gccgtttcgg tgaagagtcg cttttgtttc aactggcggg cgagctcgag
    11401 cgggcgcagc cctggtttga ccgccggccg tccctgtaac cgcagcgctc tttacccgga
    11461 ttgcctctcc gggggatgcg cgggaaagac gcctgtttga aagggaaggg aagccggccc
    11521 gggtccgttg atgcggcgat atcgacgttt ttgggatccg ccgcatcgtt ttgtgtcatg
    11581 attgacggac tcgtaaaagg tcgaaaaagc ggcaaatccg gacggcttcg taaaaactcc
    11641 gcgggcaagg cgcgcaaatc ttgaggaatg aggcgcgctt cagcgcacgc cgcaatgacg
    11701 aaggatgcgg cgcagttcag catccgggct ttttacgagg ccgtcgtgat tcgctcatta
    11761 ttctgagagc tttgaaaaat gtcatttcga ggagcgtgag cgacgagaaa tcttaagg
//
LOCUS       DAOSRM010000070        11710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5451_length_11710_cov_118.759588, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000070 DAOSRM010000000
VERSION     DAOSRM010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11710
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5451_length_11710_cov_118.759588"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1664)
                     /locus_tag="PLA58_07465"
     CDS             complement(111..1664)
                     /locus_tag="PLA58_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOR62669.1"
                     /translation="MDEKISRRNLIKRSIRVGLAAGGLAVVGAAGYKLFSGKSIDDLY
                     GPYPDNAKLKPLKLSNPSAPKPNVIIVYCDDLGYGDLGCYGNRVIRTPNIDGLARDGM
                     RFTDYYSCNAVCAPSRAGLLTGRYPFRTGIIGNPYPAENPLLRKIEREIAYQFTPMGS
                     FDQHEDYVAAGISSKETTIAEALKIAGYNTAMVGKWHLGDYSREPQFNPLKHGFDQYL
                     GVPHSNDMQPCPLFRNETQLEADIGTNQARLTGMYTREALAFIEKSKGNPFFLYFAHT
                     FPHQPLYASERFAGKSKAGKFGDAVEEIDWSVGEITKKLRELNIEQNTIIFFASDNGP
                     WYEGSPGVHRGRKGQSYEGGFRVPFIVKWPGHIRPGQVSSELAVNLDIFPTLLTLAGI
                     EHPSDRVIDGKSIEGLLSGRETVSPHDIVYFYHYDQLEGVRFGKWKYLRRLNRYVWPV
                     PLDAEYITSRMGKKALGDRWPLLYDLSLDPGENYNVIATYPDVAGKLEGMLAQWEKTA
                     EKNPRGFLE"
     gene            complement(1854..3383)
                     /gene="guaA"
                     /locus_tag="PLA58_07470"
     CDS             complement(1854..3383)
                     /gene="guaA"
                     /locus_tag="PLA58_07470"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOR62670.1"
                     /translation="MILIIDFGSQYNQLIARRVREQQVYCRIEPPDFPLSAIRAMAPE
                     GIILSGGPASIYAKGAPRADRGIFDLGIPVLGICYGLQFMVDALGGRVVGSAKREYGF
                     AELFIKKQKGLFAGVEKKTPCWMSHGDSIGELPRGFSVTASTANTKVAAAEDARRRFY
                     GLQFHPEVVHTREGGKMLANFLFGLCRCARAWSMKSFVSRAVEDIRMQVGQEKVILGL
                     SGGVDSSVAAVLLHRAIGRQLTCVFVDNGVLREGEAKRVIDMFKKHLHINLRFVRAGK
                     IFLRRLKGVTQPERKRKIIGRTFIEVFDAEARKIKGVKFLAQGTLYPDLIESRSAFGG
                     PSAVIKSHHNVGGLPKTMKLKLVEPLRHLFKDEVRLLGKELGLADDVVWRQPFPGPGL
                     AIRIIGEVTAPRLSVLKRADSILLEEIRGANLYGKLWQSFTILLPLKSVGIMGDQRTY
                     ENIVAIRAVTSDDAMTADWARLPHDLLARVSNRIINEVRGINRVVYDISSKPPGTIEW
                     E"
     gene            complement(3401..5572)
                     /locus_tag="PLA58_07475"
     CDS             complement(3401..5572)
                     /locus_tag="PLA58_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOR62671.1"
                     /translation="MKKMQPTLAAERHYVIEKEKFVPVSQYFGEDTFSHAVIKEKLPK
                     DVFKKLMQAINESKALDEETANVVAHAMKEWALEKGATHFAHWFQPMTGTTAEKHDAF
                     ADPCGPGAVIERFSGKQLVQGEPDASSFPSGGIRATFEARGYTAWDMSSPAFIKRNGI
                     STTLCIPTAFISYTGQVLDKKTPLLRSLRALNQSAVGMLKLLGAKSVKKVQVNLGIEQ
                     EYFLIDMDYYYKRQDLVLGGRTVVGAPPAKGQELEDQYFGSIRERISSFMHDVEEELY
                     KLGIPAKTRHNEVAPSQYEIAPVFEEANLAVDHNQMIMETLKDVAKKHRLAALLHEKP
                     FAKINGSGKHMNWSLSDDNGNQLLNPGKTPQDNIHFLVFLIATIRAVYRHADFLRATV
                     ASYGNDHRLGANEAPPAILSVFLGEQLTQILDNIEKGTVHKATNAEIIDLGISSLSLV
                     SKDYTDRNRTSPFAFTGNKFEFRAVGSSQSVACPAYALNTIVAESLDDLAEKIKARGS
                     KNINQAVFDVLKSEIAQVRPILFNGDNYSKEWASEAKKRGLPNEKTTPGALKALVTDR
                     ALRLFEKYQVLSNEELKARYLIHIERYLKDLEIEVNCLTNLCLTQVIPAAVAYQQKLA
                     RAILDAKEALGSAAVISSQAELLKKILDYSNDVYTACKEIAAGMQAAKALDSEQKKAE
                     MLCARVKPKMDELREYVDALENLVDDELWPLPKFWEMLFIC"
     gene            complement(5682..8285)
                     /locus_tag="PLA58_07480"
     CDS             complement(5682..8285)
                     /locus_tag="PLA58_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOR62672.1"
                     /translation="MTPYARVSTGINGLDKILNYLQMGDNVVLQVDNVEDYKKFVAPY
                     VQEALARNQRLVYMRFANHPALLEAGPGIKIYQLNANSGFESFSTEVHNIIRDEGRDV
                     FYVFDCLSDLLQAWATDLMIGNFFVITCPYLFELNTVAYFAILRSRHSFKTVARIRET
                     TQVLLDVYSHEGKICVHPLKAWKRYTPTMFLPHIMEEQACVPILNSADAASILSYLTD
                     KNATSGERNLDYWDRIFLKAGTLLGDPDAAPEKQDMVEILSRVMIGREKRILSLVREY
                     FELEDLVEIKKRLIGTGFIGGKSVGMLLARNILRKDPSFDWSAVMEIHDSFYVGSDIF
                     YSYMVQNGWWKLLMAQKTDEGYFGVARELKQKMLHGAFPDEIKEQFQLMLEYFGQAPI
                     IVRSSSLLEDAFGNAFAGKYESDFCVNQGTPEERYRHFEEAVRKIFASTMNEDALTYR
                     LQRGMAQQDEQMALLVQRVSGSHSDRYFFPGLAGVGLSYNTFVWQKGMDPKAGMLRIV
                     FGLGTRAVNRVENDYPRIVALDAPTVKPYTKPEDIKRFSQHEADVLNLRDNEFQTLQT
                     AELLREDLVAHLNLIVEEDKEAADYLRSVGRDAKQAWIITFEELLSATPFVKTMSKML
                     KTLEQVYRYPVDIEFTVNFTASGDPRINLLQCRPFQTKGHYSRVELPEKISRSRMLIR
                     QDANFMGGSVYQAISRVIYIDPRAYSGLPLTEKHEIARLTGKLNRRIGKREAMPTVLL
                     GPGRWGTTTPAMGVPVTFSEINNMTAIGEIAYQDGALIPDLSFGTHFFQDMVEMDIFY
                     MAIYPEKEGVVFNVQWIKKQPNILADLMPDDARFAGVVRVCDVRAKDVRLMSDIVTQK
                     MICFLGEAGRP"
     gene            8904..10136
                     /locus_tag="PLA58_07485"
     CDS             8904..10136
                     /locus_tag="PLA58_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOR62673.1"
                     /translation="MNSGDTAWVLTASALVLLMTIPGLAFFYGGLVRRKNVLSILMQC
                     FIIVCVISLQWVLFGYSLAFGPDFHGIIGTLEWAGLAGVGAAPNSNYAATIPHSAFMI
                     FQAMFAVITPALIIGAYAERVKFPAFLLFTVLWATFVYDPLAHWVWGAGGWLKSLGGL
                     DFAGGIVVHVSSGISALVLALLLGKRIGYNHKPIRPHNLPFTVLGAALLWFGWFGFNA
                     GSALAADGLAATAFVNTNTATAAAGLAWALIEWRHNGSPTILGAATGAVAGLVAITPA
                     CGFVNPMNAMLIGIIVAFVCYIAVAVMKGKLGYDDSLDAFGVHGVGGTVGTLLTGIFA
                     QKAINPAGADGLLFGNLHTFGIQCLMLVVTVVFAAIMTFIIFKIVDAAIGMRVEEKNE
                     IIGLDLTQQSEAAYTVIE"
     gene            10148..10489
                     /locus_tag="PLA58_07490"
     CDS             10148..10489
                     /locus_tag="PLA58_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOR62674.1"
                     /translation="MKLVIAIIQPHKLEAVKRELEAVEVNLMTVTNVLGQGRQKGVTE
                     IYRGAREAGGLLNKVRLDIAVNEDFVEPTIDAVTRGAHTGSVGDGKIFVVELAQCIRI
                     STGERGGVAIG"
     gene            10573..11472
                     /locus_tag="PLA58_07495"
     CDS             10573..11472
                     /locus_tag="PLA58_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62675.1"
                     /translation="MKTDTGKAWIMILLTILLTSFPLPAIAAEEPAPAAEARPAAEAT
                     TPTVDRITGEVTVAVLSAYIWRGQEMTRDSVVIEPSLTANYKGFTATLWGNLDTNPYS
                     AGVAKHSSNWTETDFILSYAREFGIVKAGAGYIYYGLAGMTPNGADLPDAQEFFVTLG
                     LDTLLAPTLTVYREIDHYHQWYATLGVSHTFSLHERVGLKLTATASYLKSEDETTYPK
                     FNSDSIATTDKFNNFHDGVVSVSLPVAVAGNLTITPTASYVFPLSDDARYEIRARGLK
                     GTANPSDRDSSYLYGGVTLSLTF"
     gene            complement(11482..>11710)
                     /locus_tag="PLA58_07500"
     CDS             complement(11482..>11710)
                     /locus_tag="PLA58_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62676.1"
                     /translation="EEREKLIAELQQAMSDVKVLSGMLPICASCKKIRDDKGYWNRIE
                     AYIGKHSNAQFSHGICPECARKLYPELYEKS"
BASE COUNT         2464 a         3300 c         3213 g         2733 t
ORIGIN      
        1 ccccttcagg gtcgcttgca caggctggga gtcggcttca gcagcaaaaa tttcgattgg
       61 agagccgtgc gtttttcatg aacaaatgat gtccatgttt ccgtttcccg ctactccagg
      121 aatccccgcg ggtttttttc agccgtcttt tcccattgag cgagcatgcc ttccagtttt
      181 ccggcgacat cgggataagt ggcgatgaca ttgtagtttt cgcccgggtc cagggaaaga
      241 tcgtaaagaa gcggccatct gtcccccagt gcctttttcc ccatcctgct ggtaatatat
      301 tccgcatcga ggggcaccgg ccagacgtac cggtttaatc ttctcaggta tttccatttt
      361 ccgaagcgta caccttccaa ctggtcataa tggtagaaat aaacgatgtc gtgcggcgag
      421 acggtctcac gtccggaaag aaggccttcg atgctcttcc cgtcgataac ccggtcggac
      481 ggatgttcga ttcccgccag tgtcaggaga gttggaaaaa tgtccaggtt gacggccagt
      541 tcagaagaca cctgacccgg acggatatgt cccggccatt tcacaatgaa gggcaccctg
      601 aacccgcctt cgtagctctg tcccttgcgg cctctgtgca cacctggact tccttcatac
      661 caggggccgt tatcgctggc aaagaagatg atcgtgtttt gttcaatatt cagctcccgc
      721 aattttttgg tgatttcgcc gacgctccag tcgatttctt cgacggcgtc gccgaatttt
      781 ccggcttttg attttccggc aaaccgctct gacgcgtaca gtggctggtg cggaaaagta
      841 tgggcaaaat agaggaaaaa cgggttgccc tttgactttt caatgaaggc caacgcttcc
      901 ctggtgtaca tgccggtcag cctggcctgg ttggttccga tgtccgcttc cagctgtgtt
      961 tcattgcgaa agagcggaca cggctgcatg tcgttgctgt gcgggacgcc aaggtactgg
     1021 tcaaagccat gcttgagcgg attgaattgg ggctcacggc tgtagtcgcc caggtgccat
     1081 ttccccacca tggcggtgtt gtaaccggca attttcaggg cttctgcgat ggtagtttcc
     1141 ttgctggaaa tccctgcggc gacatagtcc tcatgctgat caaaagatcc catcggtgta
     1201 aattgatagg cgatttccct ttcgatcttg cgcaaaagcg gattctccgc cggataagga
     1261 ttgccgatga tgcctgttct aaacggatag cggccggtca ggagtcctgc ccgcgatggc
     1321 gcgcacacgg cattgcatga gtaatagtcg gtaaacctca tcccgtcgcg ggcaagcccg
     1381 tcgatgttgg gggtgcgtat caccctgttg ccgtagcagc cgaggtcgcc atagcccaaa
     1441 tcgtcgcagt aaacaatgat gacattcggt ttcggggcgg aaggattgga gagcttcagt
     1501 ggcttgagct tggcgttgtc gggatatggc ccgtaaagat catcgatgga tttaccgctg
     1561 aagagtttgt aaccggcggc gccgaccacg gcaagaccgc cggcggcaag cccgacacga
     1621 atggaccttt taatcagatt acgtctggat attttttcat ccatgcctta tccctcccgt
     1681 gtcatcggac ggctaaacgt aaaatgctca agaccatgcc ggatggcgcc ccgtaataca
     1741 tttttttggc aagaactttc ttttcgtgcg gttgagtata ggaagcggcg catggtgtgt
     1801 caagaagatt ccatttccca agcagggcat cgatcttcca tgcgattttt cgcttattcc
     1861 cactcaatgg tgccgggagg cttggagctg atgtcgtaca ccacgcggtt gatcccgcgc
     1921 acctcgttga tgatgcggtt ggagacgcgc gcgagcaggt cgtgcggcag cctcgcccag
     1981 tcggcggtca tggcgtcatc gctggtgacg gcccggatcg ccacaatgtt ttcatacgtg
     2041 cgctgatcgc ccatgatgcc cacgcttttg agcggaagca aaatcgtaaa cgactgccac
     2101 aatttgccgt agagattcgc gccgcggatt tcctcaagca gaatcgaatc cgcccgcttc
     2161 agcacggaca ggcgcggagc ggtcacttcc ccgatgatgc gaatggccag acccgggccg
     2221 ggaaaaggct gccgccagac cacgtcgtcc gccagaccca actcttttcc caggagccgc
     2281 acttcgtcct tgaacaggtg ccggagcggc tccaccagct tcaacttcat ggttttgggc
     2341 agaccgccga cattgtgatg ggacttgatc accgcgctgg ggccgccgaa agccgaacgc
     2401 gattcgatca gatcggggta cagcgttccc tgcgccagaa acttcacgcc tttgattttt
     2461 cgcgcctccg cgtcgaacac ttcaataaac gtccggccga tgatcttgcg cttgcgttcg
     2521 ggttgggtca ctcctttgag cctccggaga aatatttttc ccgcccggac aaaccgcagg
     2581 ttgatgtgca ggtgtttttt gaacatgtcg atgacgcgtt ttgcctcgcc ctcccgcagg
     2641 acgccgttat cgacaaaaac acaggtcagc tgacggccga tcgcccggtg gagcagcacg
     2701 gccgcgaccg acgaatccac gccgccggaa agcccgagga tgaccttttc ctgtcccacc
     2761 tgcatgcgga tgtcttccac ggcccgcgag acgaaagatt tcatcgacca tgccctggcg
     2821 cagcggcaga gcccgaacag aaaattggcc agcattttgc cgccttcgcg ggtgtgaacc
     2881 acctcgggat ggaattgcag gccgtaaaag cgccgccggg cgtcctcggc cgccgcaacc
     2941 ttcgtattgg ctgtggaagc cgtaacggaa aatccccgcg gcagctcgcc gatggaatcg
     3001 ccgtggctca tccagcaggg cgttttcttc tccacgccgg caaagaggcc tttttgcttt
     3061 ttgatgaaaa gttcggcgaa gccgtattcg cgtttggccg atccgaccac ccgaccgccc
     3121 agcgcatcca ccatgaactg caaaccgtag cagatcccca gcaccggaat tcccagatca
     3181 aagatgccgc ggtccgcgcg cggggcgcct ttggcgtaaa tgctggccgg accgccggac
     3241 agaatgatgc cttccggcgc catcgcccgg atggcggaaa gcggaaaatc gggcggctca
     3301 atccggcagt acacctgctg ttcccgcacc cgccgcgcga tgagctgatt gtactgcgag
     3361 ccgaaatcaa taatcagaat catgaggggt tcctgtcttc ttaacagata aacaacattt
     3421 cccagaactt cggcagcggc cagagttcgt catccaccag attttccagc gcgtccacat
     3481 attctcgcag ttcgtccatt ttcggtttga ccctggcgca gagcatctcc gcctttttct
     3541 gctcgctgtc cagagctttg gcagcctgca ttccggcggc gatctcctta caggccgtat
     3601 aaacatcatt gctgtaatca agaattttct ttaacagctc cgcctgggag gagatcaccg
     3661 cggcggaccc gagggcctct ttggcatcaa gaatcgcccg cgccagcttt tgctgatagg
     3721 cgacggccgc cgggatcacc tgcgtcagac agaggttggt gaggcagttg acttcgattt
     3781 ccagatcctt caggtagcgc tcgatgtgga tcaggtagcg ggccttcagc tcctcattgg
     3841 aaagcacctg atatttttca aaaaggcgaa gcgcccggtc ggtcaccagg gcttttaacg
     3901 cgccgggcgt cgttttttca ttcggcagac cccgcttctt cgcttccgac gcccattctt
     3961 ttgaataatt gtccccgttg aacagaatcg gcctcacctg cgcgatttcg cttttcaaaa
     4021 catcaaagac cgcctgattg atgttcttcg atcccctggc cttgatcttt tccgccaggt
     4081 cgtccaggct ttccgccaca atcgtgttga gggcataagc ggggcaggca acggactgag
     4141 acgagcccac cgcgcggaat tcaaacttgt tgccggtaaa ggcaaagggc gacgtccggt
     4201 tgcggtccgt gtaatctttg ctgaccagag acagcgagga aatgcccaga tcgatgattt
     4261 ccgcgttcgt cgccttatgc acggtgcctt tttcgatatt gtccagaatt tgcgtcaact
     4321 gttcgcccaa aaacacggag aggatggcgg gcggcgcttc attggcgccc aggcgatgat
     4381 cattgccgta ggacgcgacc gtggcgcgca gaaaatccgc atgcctgtaa acggcgcgga
     4441 tcgtcgcaat caaaaatacg agaaaatgga tgttgtcctg cggcgttttg ccgggattga
     4501 gcagctggtt gccgttgtcg tcggaaagag accagttcat atgcttgccg gatccgttga
     4561 ttttggcaaa gggtttttca tgcagcaagg ctgccagacg atgcttcttg gcgacatcct
     4621 tcagcgtctc catgatcatc tggttgtggt caacggccag attggcttct tcgaacaccg
     4681 gcgcgatttc gtactggctg ggcgcgacct cgttgtgcct ggtcttggcc ggaatgccca
     4741 gcttgtaaag ctcttcctcc acgtcgtgca tgaaggacga aatcctctcc cgaatgctgc
     4801 cgaaatactg gtcttccagc tcctgacctt tggccggcgg cgcgccgacc acggtccggc
     4861 cgcccagcac caggtcctga cgcttatagt agtaatccat gtcgatcaga aaatactcct
     4921 gctcgattcc gaggttgacc tggacttttt tcacgctttt tgcccccagc agtttcagca
     4981 tccccaccgc gctttggttc aaggcgcgca gcgaccgcag aagcggcgtt ttcttgtcca
     5041 gcacctggcc cgtgtaggaa atgaaggcgg tcggaatgca aagggtcgtg gaaatcccgt
     5101 tgcgcttgat gaaggcgggc gaagacatgt cccaggccgt gtagccgcgc gcctcaaagg
     5161 tcgcccggat gccgccggag gggaaagaag aggcgtccgg ctccccctgc accagctgct
     5221 tgccggaaaa ccgctcgatc acggcgccgg ggccgcaggg atcggcaaag gcgtcgtgct
     5281 tttcggcggt ggtgcccgtc atgggctgaa accagtgcgc aaagtgggtc gcgccctttt
     5341 ccagcgccca ttccttcatg gcatgggcaa cgacattggc ggtctcttcg tccagcgctt
     5401 tgctttcatt gatcgcctgc atcaattttt taaaaacgtc tttgggaagc ttctctttaa
     5461 tgaccgcgtg gctgaaggtg tcctcgccga aatactgcga gacgggaacg aacttttcct
     5521 tttcgattac gtaatgtctt tccgccgcaa gcgtaggctg catctttttc atggctttgc
     5581 catccttttg caagagatgc tcctctttac tccaaaggcg gagtttgcgc aagcgtccgg
     5641 aaacgcctta tttgccatcc tctcccgacc ggcagggatt tttacggacg accggcttcc
     5701 cccagaaaac agatcatctt ctgggtgacg atgtccgaca tcaggcgcac gtccttcgcc
     5761 cggacgtcac agacgcggac aacgccggca aaccgcgcat cgtccggcat caggtccgcc
     5821 agaatattgg gctgtttttt gatccactgc acgttgaaga ccacgccttc cttttcggga
     5881 taaatggcca tgtaaaagat gtccatttcc accatgtcct ggaaaaagtg cgttccgaag
     5941 gacaaatcgg ggatcagggc gccgtcctgg taggcgatct cgccgatggc ggtcatgttg
     6001 ttgatttccg aaaacgtaac cggaacaccc atcgccggcg tggtcgttcc ccatcgcccc
     6061 ggccccagca gaaccgtcgg catggcctcg cgctttccaa tccgccggtt gagcttgccg
     6121 gtgagcctgg caatctcatg cttttccgta agcggcagcc cggaataggc cctgggatca
     6181 atgtagatca cgcgggagat cgcctggtaa acgctgccgc ccataaagtt ggcgtcctgc
     6241 cggatcagca ttctggagcg gctgattttt tccggcagct ccacccggct gtaatggcct
     6301 ttggtttgga aaggacggca ctgcagcagg ttgatccggg gatcgcccga ggcggtaaag
     6361 ttgacggtaa actcaatgtc caccggatag cggtaaacct gttccagcgt cttgagcatt
     6421 ttggacatgg ttttcacaaa gggcgtcgca gacagcagtt cctcaaacgt gatgatccag
     6481 gcctgcttcg cgtcccgtcc cacggagcgc agataatcgg ccgcttcctt atcttcctcc
     6541 acgatcaaat tcagatgcgc caccagatcc tcacggagca gttcggcggt ctggagggtt
     6601 tgaaattcat tgtcgcgcag attcaggaca tccgcttcgt gctgggaaaa ccgcttgata
     6661 tcttcgggtt tcgtgtaggg tttgacggtg ggcgcgtcca gggcgacgat ccgtggataa
     6721 tcattttcca cgcgattgac ggcgcgcgtg ccgagaccga acacgatccg cagcatgccg
     6781 gctttcgggt ccatgccctt ctgccagacg aaggtattgt aggacaggcc gacgcccgcc
     6841 agaccgggaa agaaataacg gtcgctgtgc gaaccggaaa cgcgttgcac cagaagcgcc
     6901 atctgttcat cctgctgcgc catcccgcgc tgcagccggt aggtgagcgc gtcctcgttc
     6961 atcgtggagg cgaagatttt gcggacggct tcctcgaaat gccggtagcg ttcctccggc
     7021 gttccctggt tgacgcagaa atcgctttcg tattttccgg caaaggcgtt gccgaaggcg
     7081 tcttccagaa gcgaactgga gcggacgatg atcggcgcct ggccgaaata ttccagcatg
     7141 agctggaact gctccttgat ctcgtcggga aacgcgccgt gcagcatctt ttgtttgagc
     7201 tcacgcgcga cgccaaaata tccttcatcg gttttttgcg ccatcagcag tttccaccag
     7261 ccgttctgca ccatgtacga atagaaaata tccgaaccga catagaagga atcatggatt
     7321 tccatcacgg cgctccagtc aaaagacgga tccttgcgca aaatgttgcg ggccaacagc
     7381 atgccgacgg atttgccgcc gatgaacccc gtgccgataa ggcgcttctt gatctccacc
     7441 aggtcttcca gctcgaagta ttccctgacc agcgagagga ttcttttttc cctgccgatc
     7501 atcacacggg aaaggatttc caccatgtcc tgcttttccg gggccgcgtc gggatctccc
     7561 agtagagtgc cggctttcag aaagattcgg tcccagtaat cgagattgcg ctcgccgctg
     7621 gtggcattct tgtccgtcag gtaggacagg atgctcgcgg catccgcgct gtttaaaatc
     7681 gggacgcagg cctgctcttc catgatgtgc ggcagaaaca tggtcggtgt gtaacgtttc
     7741 caggccttga gcggatggac gcagattttt ccttcatggc tgtaaacatc cagcaaaacc
     7801 tgcgtcgttt cgcggatccg ggccacggtt ttgaaagagt ggcggcttct caaaatggcg
     7861 aaatacgcga ccgtattgag ttcaaacaga taggggcagg tgatgacgaa gaagttgccg
     7921 atcatcaggt ccgtcgccca ggcctggagc agatcggaca ggcagtcaaa aacgtaaaag
     7981 acatcacggc cttcatcgcg gatgatgttg tggacctcgg tggaaaaaga ttcaaatccg
     8041 gaattggcgt tcagttgata gattttgatt ccgggacccg cctccagaag ggcggggtgg
     8101 ttggcaaaac gcatatagac cagacgctga ttccgggcca gggcttcctg aacatagggg
     8161 gcgacgaatt ttttgtaatc ctcaacattg tcaacctgca ggacgacatt gtcgcccatc
     8221 tgcaggtaat tgagaatttt atccaggccg ttgatgccgg tgctgactcg tgcataaggg
     8281 gtcataaaac tctgtttcct cttcttcgtt ttcaataaaa caaatttgtt cggcgcaaat
     8341 cggctctatg aaatggtccg ccgacgtccc caatggcgcc cctgttcaaa atgcaaagca
     8401 ttaacaagga cttgcgaaac atggctttgc ttcggtccag cgatccgaca tttcaccgcc
     8461 atgcttcaac tttctgccgt aaatgatttt tcaatttttg tgccatcggc gcttaaattg
     8521 cttttacgct tgttccattc cgctctttta cggttaatcc gctgaacatt tttgtgccta
     8581 tctcaaaatt gaatcctgct ttggaaaaat ttgccggcct ttgccgcgga gcaagaaagc
     8641 atcattaaaa gcacaacgtt ttcatacgga tgggagatga tttgaaacct gtcgcggaga
     8701 atcttccatt ttttaccaga caagtcattt agcccacatt ttagtttgtc atttcgtaaa
     8761 atgaaacaat attgtcgcat acagaaccgg cgctgattag ataaattctc aatgatgtca
     8821 ataactaaaa aggattggaa aatatggcac tctccttgcg ctatgcaaaa ccgaaaagaa
     8881 attttttgaa ggaggcgtcc attatgaatt ccggagatac agcgtgggtg ttaaccgcca
     8941 gcgccctggt tcttttaatg accataccgg ggctggcatt tttttatgga ggtctcgtcc
     9001 ggcgcaaaaa cgttctgtcc attttaatgc agtgcttcat tattgtctgc gtcatctccc
     9061 tgcagtgggt gctgttcggc tattccctgg cgttcggtcc ggatttccac gggatcatcg
     9121 gaacgctgga atgggcgggc cttgccggcg tgggcgccgc tcccaacagc aattacgccg
     9181 caaccattcc gcattcggct ttcatgatct ttcaggccat gttcgccgtc atcacgccgg
     9241 cgctcatcat cggcgcgtac gcggaacggg tgaagtttcc ggcgttcctg ctcttcaccg
     9301 tcctgtgggc aaccttcgtc tatgacccgc tggcgcactg ggtgtggggc gcgggcggct
     9361 ggctcaagtc tttgggaggt ttggatttcg caggcggcat cgtcgtgcac gtaagctccg
     9421 gcatttcggc cctggtcctg gcgctcctgc tgggaaagag aatcggctac aaccacaaac
     9481 ccattcgtcc gcacaacctg ccgtttaccg ttctgggcgc ggctctttta tggttcggct
     9541 ggttcggctt caacgccggc agcgcgcttg cggcggacgg cctggcggcc actgcctttg
     9601 tcaacacgaa taccgcaacc gccgcggccg gtttggcctg ggcattgatt gaatggcggc
     9661 acaacggcag cccgaccatt ctgggtgcgg ccaccggcgc ggtggcgggt ctggtcgcca
     9721 ttactcccgc ctgcggattt gtcaatccta tgaatgccat gttgatcggc atcatcgttg
     9781 ccttcgtctg ctatatcgcc gttgcggtta tgaaaggcaa actcggctac gacgactctc
     9841 tggacgcctt cggcgttcac ggcgtcggag gaaccgtcgg caccctcctg accggtatct
     9901 tcgcgcaaaa agcgattaac ccggctggcg cggacggcct gctcttcggc aacctccata
     9961 cgttcggaat tcaatgcctg atgctggtgg tgaccgtcgt ctttgccgcg atcatgacct
    10021 tcatcatctt caagatcgtg gacgcggcaa tcggcatgcg cgtcgaagag aaaaacgaaa
    10081 tcatcggtct ggacctgacc caacaaagcg aagcggctta caccgtcatc gaataggagg
    10141 aacaagcatg aaactggtca ttgccatcat tcagccccac aaactggagg ccgtcaaaag
    10201 agagctggaa gccgtggagg tcaacttaat gaccgtcacc aacgtgctgg gacagggacg
    10261 gcaaaagggc gtcacggaaa tttaccgcgg cgccagggaa gcgggcggcc tgctcaacaa
    10321 agtccgtctg gatatcgcgg taaacgaaga ttttgtcgag ccgaccatcg acgccgtcac
    10381 ccgcggcgct cataccgggt cggtgggcga cggcaagatt tttgtcgtgg agcttgccca
    10441 gtgcatccgc atcagcaccg gcgagcgggg cggcgttgcc atcgggtgaa atgcggtcat
    10501 ggatcacggg aaaatacgaa attgaccgca cacaatttga ttcagaataa acaaacagga
    10561 gattggggaa aaatgaaaac ggataccgga aaagcatgga tcatgatttt actgacaatt
    10621 ttgctgacat ccttccccct gccggccatt gccgcggaag agccggcccc ggccgctgaa
    10681 gccagacccg ccgccgaggc cacaacaccg acagtcgatc ggatcacggg cgaggtgact
    10741 gtcgccgttt tgagcgccta tatctggaga ggccaggaga tgacccggga cagcgtggtg
    10801 atcgaacctt cgttgaccgc aaactacaaa ggatttacgg ccaccctctg gggaaatctg
    10861 gatacgaatc cctattcggc cggtgtcgcg aaacattcct ccaactggac ggaaacggat
    10921 ttcatccttt cctatgcgcg tgaattcgga atcgtaaagg cgggcgcggg ctatatctat
    10981 tatggactcg ccggcatgac gccgaacggg gcggacctgc ccgatgcgca ggagttcttc
    11041 gtcacactgg gcctcgacac gcttctggct ccgacgctga ccgtttacag ggaaattgac
    11101 cactatcatc aatggtacgc gacgctgggc gtctctcata ccttcagcct tcatgaaaga
    11161 gtcggtttga aactgacggc aacggcaagc tatctgaaaa gcgaagatga aaccacctat
    11221 ccgaaattca acagcgactc catcgcgacg acggacaaat tcaacaattt tcacgacggc
    11281 gtggtttctg tttccctgcc ggttgccgtg gccgggaatc tgaccattac accgacggcc
    11341 tcctatgtct ttccgctttc ggacgatgcc agatacgaaa tacgggcgcg aggcctgaag
    11401 ggcacggcca acccgtccga ccgggacagc tcctatctgt atggaggcgt aaccctgagc
    11461 ctgacgttct aaggagggcc gttatgattt ttcgtaaagt tccggataca gttttcttgc
    11521 gcattccgga caaatgccat ggctgaactg ggcattggag tgctttccaa tgtacgcttc
    11581 aatccggttc caataccctt tgtcgtcgcg aatttttttg caggaggcgc atatcggaag
    11641 cataccgctt aacaccttga cgtccgacat ggcctgttga agttccgcaa tcagtttctc
    11701 acgctcttcc
//
LOCUS       DAOSRM010000071        11696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5462_length_11696_cov_97.365175, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000071 DAOSRM010000000
VERSION     DAOSRM010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5462_length_11696_cov_97.365175"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLA58_07505"
     CDS             complement(<1..860)
                     /locus_tag="PLA58_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR62677.1"
                     /translation="MHTFTLDKPDNLIDWWQESEAKFAERRVFGTKNKSGVYEWVTYR
                     EVGARIHNLRAGLAQLDISQGDVVGIIANNRVEWPIVAFAAWGRIARFVPMYEAELVQ
                     IWKYIIADSRIKVLFVSKPEIYEKIKDFPKDISTLKHIFVIDAEGDNSMAALEKKGAA
                     KPVAAQVPKPDDIAELIYTSGTTGNPKGVLLSHGNFTSNSLAGLKMYPELAENEGRDV
                     VTLAILPWAHSFGQTGELYAVIRLGGSIGLAESPATIVDDIAKVKPTFLVAVPRVFNR
                     IYDGLWAKMN"
     gene            1094..1744
                     /locus_tag="PLA58_07510"
     CDS             1094..1744
                     /locus_tag="PLA58_07510"
                     /inference="COORDINATES: protein motif:HMM:NF004981.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HOR62678.1"
                     /translation="MIDLHTHTLFSDGALVPAESARRACAAGYRTIAFTDHADYSNID
                     FIIPRIVRVCSKISEKGNIFAIPGVELTHVDPYDIVALTFEARKLGAKIVVVHGETLT
                     EPVSPGTNLAAIRAGVDILAHPGLITAQEAKLAAKKGVFLEITTRRGHSYTNGHVAAM
                     AGKCGARLVLNNDAHAPGDFVGRPLAMNIARGAGLSEKEIVIMLKNSQKIVKRVLA"
     gene            1741..2994
                     /locus_tag="PLA58_07515"
     CDS             1741..2994
                     /locus_tag="PLA58_07515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOR62679.1"
                     /translation="MKKKSSSGPLKICLLTYRGNPTCGGQGVYIRHLSKALADEGHQV
                     DVISGPPYPHLDDPVRLIQMPGLDLYHPDHLFWPEHFGDLVHPLNLYEFLSVCAGGFP
                     EPYTFGERVYDYLKKHRGDYDIVHDNQCLSYGIGRLAQNVLPTIPTIHHPITVDRQEE
                     YKAAKTLRQKFRVYRWYSFINMQLKVAKSFSHIITVSSFTRKDIAKEFALDENRFRVV
                     HNGINNEFFYPKQNGPRPENSLIVTNSADTPLKGLRYLLEAVDQIRRKRPIKLTVIGQ
                     PKKDGIIENLVASLGLSDVVHFTGRIANEEFADYYAHSTMAVVPSLYEGFGIPAAEAM
                     ACGVPLVSTSGGALPEVVGDAGIIVPPADAKALAQAIEHLLDAPEERKRYAQAGLARV
                     HSVFSWKKAARESADVYREAIHDYH"
     gene            2981..3682
                     /locus_tag="PLA58_07520"
     CDS             2981..3682
                     /locus_tag="PLA58_07520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR62680.1"
                     /translation="MITIDLHELKLKPGFAVLDAGCGTGRHLRALARLPGLKIHGIDR
                     NPADVAKAVRALREMPDALSREVSVTSADITQMPFADETFDCVICSEVLEHIPEHEAA
                     LKELVRVLKPRGTLVVSVPRYFSERICWLISPAYASDEGGHIRIYKKKDLRRMLAGQG
                     MKCWKINYKHALHAPYWWLKCLVGIKNDDNYFVKLYHQFLVWDMFCKPRAVRVLEEAL
                     NPVIGKSIVFYLRKG"
     gene            3687..4718
                     /locus_tag="PLA58_07525"
     CDS             3687..4718
                     /locus_tag="PLA58_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62681.1"
                     /translation="MELNISLSEELKPIDVEKVADFIASLQKGNGEIPWSQGGKTDPW
                     DHIESAMGLSVAGFIDEAERAYAWLVSTQLPDGSWWSEIRDGVVTNSTKETHFAAYIA
                     VGVYHHYLITHNEKFLRTMWPALSRGIQYALGLQAPEGEIYWARNREGAIDKMALLTG
                     CSSIYMSLKCALAIADVLDIKRPAWQKAKERLGETIRNRPDLFNMIKSRFSMDWYYPI
                     LCGAVRGSEAKARIDHLWDKFVVPDWGVRCVSDRPWVTMAETSEFILTLAAMEDLTRA
                     RAIFSWLGDKRYPDGSYWMGVTFPDTVIWPEEKTGWTAAAVLLAWDALNGMTPASKIF
                     AHNRWPEKP"
     gene            4805..5527
                     /locus_tag="PLA58_07530"
     CDS             4805..5527
                     /locus_tag="PLA58_07530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOR62682.1"
                     /translation="MLKDHRPYYIKRFYNKFQKMYARYFLAPQFEVFGRGLVFMKPWH
                     VEIFGGPVSLGDYSNVIATPDKKVRLTVWPAWKSSGRIEIGRCCLICPGTRIMAATSV
                     TMGDGCMTAQHVSISDSDWHDLYDRSQPVGVTQAVRIGNNVWLGENAVICKGVTIGDN
                     AVIGAGSIVVKDIPANAIAAGNPAAVIKYLDPDKPIRTRMEWLANPAKLAEEFEEIDR
                     DMMKGNTMIGWFRSLLFPRKGD"
     gene            5532..7100
                     /locus_tag="PLA58_07535"
     CDS             5532..7100
                     /locus_tag="PLA58_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR62683.1"
                     /translation="MRVAVIAAPYPLEEFPSPPLGIAYVAAAFEAAGCEVRVFDYIIS
                     HYSKEKLAAQLAEFQPDAVGAGSVTMNFYDGQRILRDVKSIDPGILTMMGGPHVSFTV
                     EETLRTYPEIDVIFIGEADDTIVEFAPLIRQKNQWPQVRGIAFREGNEIVNTGRRDFI
                     ADVDRIPLPARRLLPISRYRALGYPVSMITGRGCPHGCIFCLGRKMVGAKVRRRNPAL
                     VLDEIEQVLNLGFDRMNIADDLFASDTDRVREICRGIRERGLKFTWSAFARVDTVTQE
                     MFDEMASVGCDSVSFGVESGCPEMLKRVRKGITLQQAAEAVQMCKKAGMLAHASFMVG
                     LPGETKETLQRTDDFARSLDIMYGYHYLAPFPGTTLCEKVERYDLQILTRDWSKYDAN
                     DAIVKTSELVPQDIRDFVGRYEAEVSGDWQKVVDNYNKGINTPYDDLRVEGQKRTKIT
                     YAVLKEDLVENLGVIEPSLAGGKDAVENLLIERMKTSVSGDPLLIEKTLRDFMAKGYL
                     SADFSDKGCVWRWT"
     gene            complement(7108..7740)
                     /locus_tag="PLA58_07540"
     CDS             complement(7108..7740)
                     /locus_tag="PLA58_07540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR62684.1"
                     /translation="MVKIDPIKISKIKGFLDEAEAERLYELALEASRSSPCLEIGSYC
                     GKSAVYLGCACRENGAVLFSLDHHTGSEEQQPGQEYFDPDLLDKETGKIDTFRLFRKT
                     IEEFDLQDTVIPVIGRSDIIGRAWKTPLGLVFVDGSHAYESVLKDYQIWADHILPGGY
                     LVFHDIFPNPADGGQAPYLVYRQAVDSGLFDAAPLFKSLGILKKRLKAEG"
     gene            7919..9499
                     /locus_tag="PLA58_07545"
     CDS             7919..9499
                     /locus_tag="PLA58_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOR62685.1"
                     /translation="MQEKNRNQSSMDADHVRRLTQIGVALSAEKNIDRLLEKILSEAR
                     TITSADGGTLYLMSENERELQFAFIQNESMGVTMGGTGEKISWPPVPMTGADGLPNHA
                     NVSAHVAITRQAVNIEDVYDASGFNFDGPRAFDQKTGYRTKSMLVVPMLNHENEIIGV
                     LQMINARDKETGGVIPFSERARDLAWSLASQAAVALTNNLLILELQTLLEAFIQTIAL
                     AIDEKSPYTGGHVRRVAGLTMKIAEAVNDCRDGHYANTRLSEDEMRELRMAAWLHDVG
                     KITTPDHVVDKATKLEKVYDRIGEITTRFEVLKRDRLLAARDGRENPSGPEAGQTERE
                     LQELDEERRFLLQMNIGGEFTKDQMIERIRQIARRQWMSDGELRPLLSEDEVYNLSIR
                     RGTLNDEEREIINNHAALTYKMLSKLPFPKKLRRISEYAGAHHEKLDGSGYPLGLKGD
                     ELSLQSRIIALADIFEALTAKDRPYKKGKTLGEALKIMEMMVKDHHIDANLYDLFVRE
                     KIYSDYAASELAPQQMDV"
     gene            9804..10979
                     /gene="metK"
                     /locus_tag="PLA58_07550"
     CDS             9804..10979
                     /gene="metK"
                     /locus_tag="PLA58_07550"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOR62686.1"
                     /translation="MQISSESVKVGHPDLVADIIAANVIAAILDEEKKQKLTLANMPH
                     CGIEVFLGKGICMVGGEVRSRVYVDIDKIARDSVIELGYNHAAVGLNGHLMGVLNAII
                     PQSPDINQGTSCLLNKDREIGAGDQGIMYGFACDETPEMLPLPYVLVNKLMRAFEYCQ
                     DPIFAPDGKGQVSVDYDVKTGKPCRVAKVLMSNSIDYRFVKGDRLKIRDRAKKIAFDV
                     LKDCVDKKTEFLFNPTGEWNAINSCSAADSGVTGRKLVVQFYGGYPGAQLGGGAVVNK
                     TPEKVDCSAAFGARHVAKNIVAAGLATKCSVQLAYAIGIAEPFSIYVNTFGTGKIPDD
                     RLEKIIRDLFDLTPEGMIEKFNLLDGNIYRRIPRTFFMDDYRWEKTDMAKTLQKAVK"
     gene            11029..>11696
                     /locus_tag="PLA58_07555"
     CDS             11029..>11696
                     /locus_tag="PLA58_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOR62687.1"
                     /translation="MAAIWSQENKYAKWLDVEVAACEAMVKLGLAPAAAVDNIKAKAV
                     INAARIDEIEAVTRHDVIAFVSSLTEVIGEDGRFIHMGLTSSDVLDTALAVQLKEASD
                     LLLEDLDDLLAVLKRRALEHKKTLMIGRSHGIHAEPITFGLKLALWYAEMRRNRARLE
                     RAREAVSCGKISGAVGTFSFVDPFVEEYVCEKLGLAPAPVASQIVQRDRHAEFFSTLA
                     IVASS"
BASE COUNT         2678 a         3109 c         3460 g         2449 t
ORIGIN      
        1 ttgttcattt tggcccagag gccgtcgtag atgcggttga aaacgcgggg cacggcaacc
       61 agaaaggtgg gtttcacctt ggcaatgtca tcgacaatcg tcgcggggct ttccgccagc
      121 ccgatggagc cgcccaatcg gatgaccgcg tacagttcgc cggtctgacc gaaagaatgg
      181 gcccagggca ggatggcgag cgtcaccacg tcccggcctt cgttttccgc cagttcggga
      241 tacattttca ggcccgccag agagttgctg gtaaaattgc cgtgcgacaa aagaacacct
      301 ttgggattgc cggtagttcc cgacgtgtag atgagttcgg cgatgtcgtc cggttttggg
      361 acctgtgcgg cgaccggctt ggcggcgcct ttcttttcca gcgcggccat ggaattatcg
      421 ccttcggcgt cgatgacaaa aatatgcttc agcgtggaaa tatccttcgg gaagtccttt
      481 attttctcat agatttccgg tttggaaaca aacaggactt taatccggct gtcggcaatg
      541 atgtatttcc agatctgcac cagttccgct tcgtacatgg ggacaaagcg ggcgatgcgt
      601 ccccaggcgg cgaaagccac aatgggccat tccacgcggt tgttggcgat aatgccgacg
      661 acatccccct ggctgatgtc cagttgggcc agtcccgccc gaaggttatg gatgcgggcg
      721 cccacctccc tgtacgtgac ccattcataa acgccgcttt tgttttttgt gccgaaaact
      781 cttcgctcgg caaattttgc ttcgctctcc tgccaccagt caatcaggtt gtcaggctta
      841 tccagtgtaa acgtgtgcat aaacgatctc cttctagtct attgtcgcgg ggggtatttc
      901 cgtccctcga aatcatcgaa aaaatgcctg ctgcgtcatt tcgaggatgg tctaagtcat
      961 tttcacggaa aaaacaagca aaaccattga tttaattttc agaaaattca tcattttatt
     1021 gactgtgtgt ttcaaatttg ctaagtgacc actagttttt ggaagtgaaa cgaaagctta
     1081 tggacggctg tacgtgattg atctgcatac ccataccctt ttcagcgacg gcgcgcttgt
     1141 tcccgcggaa tcggcccgga gggcctgtgc cgcgggatac cggacgattg cgttcacgga
     1201 ccatgcggat tattccaata tcgatttcat tatcccgcga atagtcaggg tttgtagcaa
     1261 gatatcggaa aaagggaaca ttttcgcaat accgggcgtg gaactgacgc atgtggatcc
     1321 ctacgacatc gtcgcgctca ctttcgaggc ccgaaagctg ggggcgaaaa tagtggttgt
     1381 gcacggggaa accctcacgg agccggtttc ccccgggacc aatctggccg ccatccgcgc
     1441 gggagtggat attcttgctc atcccgggct gattacggcc caagaggcaa aactggccgc
     1501 gaaaaaaggt gtttttctgg agatcaccac ccgccggggg catagttaca cgaacggcca
     1561 tgtggcggcg atggccggaa aatgcggggc ccggcttgtg ctcaacaacg acgctcacgc
     1621 gccgggcgat tttgtgggcc gccctctggc catgaacatt gcccgcggcg ccggcttgtc
     1681 cgagaaagaa atagtcatca tgttaaaaaa ttcccaaaag attgtaaaaa gggtcctggc
     1741 gtgaaaaaaa aatcaagttc agggccgttg aagatttgtc tgctcacgta tcgtggcaac
     1801 cccacctgcg gagggcaggg cgtctatatc aggcatttga gcaaagcgct ggccgatgaa
     1861 ggccatcagg tggacgtcat ctccggaccg ccctatccgc atctcgacga tcctgtccgc
     1921 ctgattcaaa tgcccggctt ggatctctat catcccgacc atctgttctg gccggaacat
     1981 ttcggggatc tggtccatcc gctgaacctg tatgagtttt tgagcgtctg cgcgggcggt
     2041 tttcccgagc cctatacctt cggagagcgg gtctatgact atctgaagaa acaccgcggc
     2101 gattacgaca ttgttcatga caaccagtgc ctgtcttacg gcatcggccg gctggcgcaa
     2161 aacgttttgc cgacgatccc cacgattcac catccgatca cggtggaccg gcaggaggag
     2221 tacaaggcgg ccaaaacgct gcgccagaaa ttccgggtgt accgctggta ttcgttcatc
     2281 aatatgcagc tgaaggtcgc caaaagcttt tcgcacatca ttaccgtgtc ctccttcacc
     2341 cgaaaggaca ttgccaaaga gtttgccctg gatgaaaaca gattccgggt tgtgcacaac
     2401 ggcatcaaca atgagttctt ttatcccaag caaaacggac cccggccgga aaattccctg
     2461 atcgtgacca acagcgcgga tacgccgctc aagggactga ggtatcttct ggaggcggtg
     2521 gatcagatcc gccgcaagcg gccgatcaag ctgacggtca tcggccagcc caaaaaagac
     2581 ggcatcattg aaaacctggt ggccagtctc gggttgtccg atgtcgtgca ttttaccgga
     2641 cgaattgcca acgaggaatt tgccgattat tacgcccatt cgacgatggc cgtcgtgccg
     2701 tcactgtacg agggcttcgg cattcccgcg gctgaggcga tggcctgcgg cgtgccgctc
     2761 gtttccacct ccggcggggc gcttccggaa gtggtggggg atgccggcat tatcgttccg
     2821 cccgccgacg cgaaagcgct ggcgcaggcg atcgagcatc tgctggatgc tcccgaagaa
     2881 agaaagagat acgcgcaggc gggactggcg cgcgtgcatt ccgtgttcag ctggaagaaa
     2941 gccgcccggg aatcggccga cgtctatcgc gaggcgattc atgattacca ttgatttgca
     3001 tgaactcaag ctgaagccgg gattcgcggt tctggacgcc gggtgcggca ccggcaggca
     3061 tttgcgcgcg cttgcgagac tgccgggttt aaaaatccat gggatcgacc ggaatcccgc
     3121 cgatgtggcc aaggcggtgc gggcgcttcg ggagatgccg gatgcgcttt cccgggaggt
     3181 ttccgtgacg agcgccgaca ttacgcaaat gcccttcgcg gacgaaacct ttgactgtgt
     3241 gatctgctcc gaagtgctgg agcacatccc ggagcacgaa gcggccctga aggaactggt
     3301 ccgtgtgctg aagcccagag gaaccctggt ggtcagtgtt ccgcgctatt tttccgagcg
     3361 catctgctgg ctgatttctc cggcgtacgc aagcgatgaa ggcgggcaca tccgcattta
     3421 taagaagaaa gacctgcgca ggatgctggc ggggcagggg atgaagtgct ggaaaatcaa
     3481 ctacaaacac gcgctgcacg cgccctactg gtggttgaaa tgcctggtcg gcatcaagaa
     3541 cgatgataat tattttgtga agctttatca tcaatttctg gtttgggaca tgttttgcaa
     3601 gccaagggcg gtgcgggttc tggaagaagc gttgaatccg gtcatcggaa agagcattgt
     3661 gttttatctg agaaaaggct gaccgtatgg aattgaatat atcgctgagc gaagagctca
     3721 aacccattga cgtggaaaag gtggccgact tcattgcctc tctgcaaaaa ggaaacggcg
     3781 agatcccctg gtcgcagggc ggcaagacgg acccctggga tcatatcgaa agcgccatgg
     3841 gattgagtgt cgccggattt atcgacgagg cggagcgcgc ctatgcctgg ctggtttcca
     3901 ctcagttgcc ggatgggagc tggtggtcgg aaatccgcga cggcgtggtc accaattcga
     3961 cgaaggaaac tcattttgcc gcctacatcg ccgtgggcgt ttatcatcac tatctgatca
     4021 cccacaacga aaaatttctt cgaaccatgt ggcctgcgct gtcgcgcggc attcaatacg
     4081 cgctgggtct gcaggcgccg gagggagaaa tctactgggc gcgaaaccgc gaaggcgcca
     4141 tcgacaagat ggcgctcctc accggttgca gttccattta catgagcctc aaatgcgcgc
     4201 tggccatcgc ggacgtgctg gacatcaaaa gacccgcctg gcagaaggcc aaagagcggc
     4261 tgggagagac gattcgcaac cggcccgatc tgttcaacat gatcaagtcc cgtttttcga
     4321 tggactggta ctatcccatc ctgtgcgggg ccgtccgggg aagtgaggcc aaagcccgaa
     4381 tcgatcattt gtgggacaaa tttgttgtgc ccgactgggg ggtgcgctgc gtcagcgacc
     4441 gtccctgggt gaccatggcc gaaacatcgg aatttatcct gacgcttgcc gccatggaag
     4501 accttacccg cgcccgggcc atcttcagct ggcttggcga caaacgctat ccggatggct
     4561 cctactggat gggcgtgacg tttcccgaca ccgtgatctg gccggaggaa aaaaccggct
     4621 ggacggccgc ggcggttttg ctggcctggg atgccctcaa cggcatgaca cctgcctcaa
     4681 aaatattcgc ccacaaccgc tggccggaga aaccctgatg cattcctgaa aacggcgcca
     4741 gactcccggc ggcggggttt gcgaatttcg ttgtccgccg cgccattttg aaaagagtaa
     4801 gacattgctg aaagatcatc gaccctacta catcaagcgc ttttacaata agttccagaa
     4861 gatgtatgcc cggtattttc tggcgccgca gttcgaagtt ttcggccgcg ggctggtctt
     4921 tatgaagccc tggcacgtgg aaatattcgg cgggccggtt tccctggggg attattccaa
     4981 tgtcatcgcc accccggaca agaaagtgag gctgaccgtc tggccggcct ggaagagttc
     5041 cggaagaatt gaaatcggcc ggtgctgttt gatctgcccg gggacgagaa tcatggcggc
     5101 cacctcggtc acgatgggcg acggctgcat gacggcccag catgtcagca tcagcgattc
     5161 cgactggcat gatctgtacg atcgcagtca gccggtgggc gtcacccagg cggtccgcat
     5221 cggcaacaac gtctggctgg gcgagaacgc cgtgatctgc aagggggtga cgatcgggga
     5281 caacgccgtg atcggcgccg gttccattgt ggtcaaggac attccggcca acgcgattgc
     5341 cgccgggaat ccggcggcgg tgatcaaata tctggatccg gacaaaccga tccggacgcg
     5401 catggaatgg ctggccaatc ccgccaaact ggcagaagaa tttgaagaga ttgatcgcga
     5461 catgatgaaa ggcaacacga tgatcggctg gttccgatcg ctgcttttcc cgcggaaagg
     5521 agattgaccc catgcgcgtt gccgtgattg ccgcccctta cccgctggaa gaattcccct
     5581 cgccgcccct gggcatcgcc tatgtcgccg cggcctttga agcggcgggc tgcgaagtgc
     5641 gggttttcga ctatatcatc tcccattatt ccaaagaaaa actggccgct caactggccg
     5701 aatttcaacc ggatgccgtg ggcgccggtt cggtcacgat gaatttttac gatggacagc
     5761 ggattctgcg cgatgtgaaa agcatcgatc ccggcatcct cacgatgatg ggcggacccc
     5821 acgtatcctt taccgtggaa gagacgctgc gaacctatcc ggaaatcgac gtgattttta
     5881 tcggcgaggc ggatgatacc atcgtcgaat tcgcgccgct gatccggcaa aaaaatcaat
     5941 ggccgcaggt gcggggcatt gcgtttcgtg aaggcaatga gatcgtcaac accggccgga
     6001 gagatttcat tgctgatgtc gatcgcattc cgcttcccgc ccgccgtctg ctgcccatct
     6061 cccgctaccg ggcgctggga tatcccgtca gcatgattac cggccgcggg tgtccgcacg
     6121 gctgcatttt ttgtctggga agaaaaatgg tcggcgcgaa agtccgccgc cgcaatccgg
     6181 ctctggtgct cgatgagatc gagcaggttc tgaatttggg ctttgaccgg atgaacatcg
     6241 ccgacgacct gtttgcgtcg gacaccgacc gcgttcggga aatctgccgg ggcatccggg
     6301 agcgcggcct gaaattcacc tggagcgcct ttgcgcgcgt ggacaccgtt acgcaggaaa
     6361 tgtttgacga gatggcgtcc gtgggctgcg acagcgtcag cttcggcgtg gagtcgggct
     6421 gtccggaaat gctcaaaagg gtgcgcaagg gcattaccct tcaacaggcg gccgaggccg
     6481 tccaaatgtg caagaaggcg gggatgctgg cgcatgcctc ctttatggtc gggctgccgg
     6541 gcgaaacgaa ggagacgctt cagcggacgg atgactttgc ccgcagcctg gatatcatgt
     6601 acggctacca ctacctggcg cctttcccgg gcacgaccct gtgcgagaaa gtggagcgct
     6661 acgatctgca aattctgacc cgggactggt ccaaatatga cgccaacgac gcgatcgtga
     6721 aaacttcgga gcttgttccc caggacatcc gggattttgt cggccggtat gaagccgagg
     6781 tgagcggcga ctggcagaag gtggtggaca actacaataa gggcatcaac acgccctacg
     6841 acgatttgcg ggttgaaggc cagaagcgga cgaagattac ctatgccgtt ctcaaggaag
     6901 accttgtgga aaatctgggc gtcatcgagc cgtccctggc cggcggaaaa gacgcggtgg
     6961 aaaacctgct gatcgaacga atgaaaacct ctgtttccgg cgatccgctg ctgatcgaaa
     7021 aaaccctgcg ggattttatg gccaaaggat atctgagcgc cgatttcagc gacaagggct
     7081 gtgtctggcg ctggacataa caagccttca gccttcagcc ttcagccttt ttttcaaaat
     7141 cccgagcgat ttgaaaagcg gagcggcgtc gaagaggccc gagtccaccg cctgccgata
     7201 aaccagatag ggcgcctgac cgccgtcggc gggatttgga aaaatgtcgt gaaaaaccag
     7261 ataaccgccg ggtagaatgt ggtccgccca aatttgataa tccttcagaa cggattcgta
     7321 agcgtggctg ccgtccacaa agacaaggcc aagcggcgtc ttccaggcgc gtccgatgat
     7381 gtccgatcgg ccgatcaccg ggatgacggt gtcctgaaga tcgaattctt caatcgtttt
     7441 gcgaaacagg cggaacgtgt cgatttttcc cgtttcttta tccagcagat cgggatcgaa
     7501 gtactcctga cccggctgct gctcctctga gccggtgtgg tggtccagtg aaaaaagcac
     7561 agccccgttt tcccggcagg cgcatcccag atagacggcg gattttccgc agtagctgcc
     7621 gatttccaga cagggagacg accgggaggc ttccagggcc agttcataga gacgctcggc
     7681 ttccgcctca tccagaaatc ctttgatttt tgatattttg atggggtcaa tctttaccat
     7741 cggcgatcct taaaatagaa agtgcctttc attaaaccgc ccccggataa attgcaacac
     7801 aaagatggcg gaaaacgatg tcatcttttc cgcagggttt cgcaagttcg ctgttgacag
     7861 agatgcctgt tttagagata gtacaaaaaa atgatcgatc agggcgggcg gtgcattgat
     7921 gcaggaaaaa aacaggaacc agagttcgat ggatgcggac cacgtaagaa ggctgacgca
     7981 aatcggcgtg gcgctatccg cggaaaagaa tatcgaccgt cttctggaaa aaattctatc
     8041 ggaagcccgc accattacct ccgcggacgg aggcacactg tatctgatgt cggaaaacga
     8101 gagagagctt cagttcgcct tcatccagaa tgaatccatg ggtgtgacga tggggggaac
     8161 aggcgagaaa atttcctggc cgcccgtgcc catgaccggc gccgacgggc tgcccaatca
     8221 tgcaaacgtt tccgcccatg tggcgattac ccggcaggcg gtgaacatcg aggatgttta
     8281 cgacgcgtcc ggattcaatt ttgacggtcc ccgggcgttc gaccagaaga cgggctaccg
     8341 cacgaaatcc atgctggtcg ttccgatgct caatcacgag aatgaaatca tcggcgtttt
     8401 gcagatgatc aacgcgcgcg ataaggaaac gggcggtgtg atcccgtttt ccgaaagggc
     8461 ccgggatctg gcctggtctc tggcctcgca ggcggcggtg gcgctcacca ataacctgct
     8521 gattcttgaa ctgcaaaccc tgctggaagc tttcattcaa accatcgcgc tggccatcga
     8581 tgaaaaatcg ccttacaccg gcggccatgt ccggcgcgtg gccggcctga ccatgaagat
     8641 tgccgaagcc gtcaacgact gccgcgacgg gcattacgca aacacccgcc tgtccgagga
     8701 tgaaatgagg gagctgcgga tggcggcctg gctgcacgat gtcggaaaaa tcaccacgcc
     8761 ggaccatgtc gtggacaagg ccaccaagct ggagaaggtg tacgaccgca tcggtgaaat
     8821 cacaacgcgg ttcgaggtgc tgaaaaggga ccgtctgctc gcagcgcgcg acggacggga
     8881 aaacccgtcc ggtcccgaag cagggcagac ggaaagggaa ctgcaggagc tggacgaaga
     8941 gcgacgtttt ctgctgcaga tgaacatcgg aggcgaattc acgaaggatc agatgatcga
     9001 acgcattcgg cagatcgccc gaagacaatg gatgagcgac ggggagctcc ggccgctgct
     9061 ttcggaagac gaagtgtaca acctgagcat ccggcgcggg acgctgaatg acgaggaaag
     9121 ggagatcatc aacaaccacg cggccctgac ctataagatg ctctcgaagc tgcctttccc
     9181 caaaaagctg agaagaatct ccgaatacgc gggcgcgcat catgaaaagc tggacggcag
     9241 cggctatccg ctggggctca agggcgacga gctttccctt cagtcgagaa tcatcgcgct
     9301 tgccgacata tttgaagcgc tgaccgctaa ggaccggccg tacaaaaaag gcaagacgct
     9361 tggcgaagct ttgaaaatca tggaaatgat ggtcaaggat caccacatcg acgccaacct
     9421 ctacgacctg ttcgtccggg aaaaaattta cagcgactac gccgcaagcg agctcgcgcc
     9481 gcaacagatg gacgtataaa aaaaaggggc taggctgaag accgaagact gaagactgaa
     9541 gactgaaggg tgaaggctga aggctgaagg cgggaggcgg gaggcgggag gcgggaggtt
     9601 gtttagcagg aaatatttac ttgattttat gtcctgatgt ggtatgaagc ccctgcaagc
     9661 ggtgatttaa tggtaaattg ctccgctcta taaatgtgct aaagcactga aaatcaatga
     9721 aaaccattga acccgtgctt tggcgcgggt tttttattct acatgcaaaa cccgcacaaa
     9781 aaactgtaag gagaagatgt tttatgcaga tttcatcgga aagcgttaaa gtgggccatc
     9841 cggacctggt ggcggatatt attgcggcga atgtgattgc ggccattctg gatgaagaaa
     9901 agaaacaaaa gctgacactg gccaatatgc cgcactgcgg cattgaagta tttctgggca
     9961 agggaatatg catggtgggg ggggaggttc gctcgagagt gtatgtggac atcgacaaaa
    10021 tcgcccgcga ctccgtgatt gaactggggt acaaccatgc ggcggtgggc ctcaacggac
    10081 atttgatggg cgtgctcaac gccattattc cccagtcacc ggacatcaac cagggaacca
    10141 gttgtctttt aaacaaagac cgggagatcg gcgcggggga ccagggcatc atgtacggtt
    10201 ttgcctgcga tgaaacgccg gaaatgcttc cgctgcccta tgttctggtc aacaagctga
    10261 tgcgggcctt tgaatactgt caggatccga tctttgcgcc ggatggaaaa gggcaggtgt
    10321 ccgtcgatta cgacgtcaaa acgggcaagc cctgtcgtgt cgccaaggtg ctgatgtcca
    10381 attcgatcga ttaccgtttt gtcaaaggcg accgtctcaa aatccgcgac cgggccaaaa
    10441 agatcgcttt tgatgtcctg aaagactgtg tggataaaaa aacggaattt cttttcaatc
    10501 cgacggggga atggaacgcc atcaattcct gcagcgcggc ggattccggc gtcacgggcc
    10561 gcaagctggt ggtccagttt tacggcggat atccgggagc gcagctgggc ggcggcgcgg
    10621 tggtcaacaa aacgcccgaa aaggtggact gctccgcggc tttcggcgcg cgccatgtgg
    10681 ccaagaatat cgtggcggcg ggccttgcca ccaagtgctc cgttcagttg gcctatgcca
    10741 tcgggattgc cgagcctttt tctatctacg tcaatacctt cggcacgggc aaaattccgg
    10801 acgaccggct ggaaaaaatc atcagggatc tttttgatct gacgccggag ggcatgatcg
    10861 aaaaattcaa tctgctggac ggcaacatct accgcaggat tccccggacc ttcttcatgg
    10921 acgactaccg ctgggaaaaa acggatatgg ccaaaacgct gcaaaaggcc gtaaagtaaa
    10981 acaggcaacg aggagaaagt tgtgattccg cgatattcaa gagaaaaaat ggcggcgatc
    11041 tggagccagg aaaacaagta cgccaagtgg ctggatgtgg aagtggcggc ctgcgaggcg
    11101 atggtgaagc tcggcctcgc gccggccgcg gcggtggaca acatcaaagc caaagcggtg
    11161 atcaacgcag cccgcatcga tgaaatcgaa gcggtcaccc ggcatgacgt gatcgcgttt
    11221 gtttcgtcgc tgaccgaggt catcggcgaa gacggccgtt ttattcatat ggggcttacc
    11281 tcctcggatg ttctggacac ggcgctggcg gtgcagctca aagaggcgtc cgaccttctt
    11341 ctggaagatc tggatgacct gctggcggtt ctgaaaagac gggccctgga gcataaaaag
    11401 accctgatga tcggccggtc gcacggcatt cacgccgagc cgatcacctt cggcttgaaa
    11461 ctggccctgt ggtatgcgga gatgcggcgc aaccgcgcgc gcctggagcg cgcgcgcgag
    11521 gccgtctctt gcggcaaaat ctcgggcgcc gtgggcacgt tttcttttgt ggatccgttt
    11581 gtggaagagt atgtctgcga aaagctcggc ctcgcgcctg ctccggtcgc gtctcagatt
    11641 gttcagcggg accggcatgc ggaatttttt tccacgctgg cgattgtcgc gtcttc
//
LOCUS       DAOSRM010000072        11328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5652_length_11328_cov_111.653508, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000072 DAOSRM010000000
VERSION     DAOSRM010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11328
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5652_length_11328_cov_111.653508"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLA58_07560"
     CDS             complement(<1..424)
                     /locus_tag="PLA58_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOR62688.1"
                     /translation="MPYKRKPRLPNFDYLGRHRYFITICTDQKQPLLEDSERVNAAIA
                     VLAEKSHEHGFTVWGYCFMPDHLHLLLEGEREDADMQAFVRGFKQKTGYRYRSETTAA
                     RQLWQPGYYDRVLRRDEDLQEVLKYIFNNPVRKGLVGHY"
     gene            complement(552..3467)
                     /locus_tag="PLA58_07565"
     CDS             complement(552..3467)
                     /locus_tag="PLA58_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOR62689.1"
                     /translation="MMKRLFCTLVVGIVFSGIAGAAEPPKEPILKIETGMHTAFIRNI
                     SVDDANRYLVTGSHDKSVRVWELATGRLLSVLRPPIGEGNEGRIDAVAISPDGQTVAC
                     GGWTQFAGPEGGLADDGVSIYLFHRATGKLTRRITGLPNVIFHLSFSPDGLYLAATLG
                     KNNGIRVFRTADGALVGEDRDYGADSYYVVFDRRGRLAAASFDGYIRLYDRPGADGLK
                     LLAKQKAPGGKRSFFLSFSPDNKQPFSLSFSPDGEKLAVGFNDSTAVDVLSASDLSHL
                     YSPDTSGVDNGNLGRVSFSPDGRLYAGGTYYKNGQTLILSWEKEGRGQRRELPGALNT
                     IMHFMPLATGGVAYGSGDPAFGVLDKNGRRVLFQSAQIADYRGNREGFRLSSDGKKVS
                     FGYEYGGNSPATFDLSTLMLSPDGDTSDLYPPDTNSLSVTDWKHATSPKLNGRALALK
                     QYEISRSVAISPSRDAFLLGADWSLRYFDRSGNEKWQAPSPGVTWAVNISRDGRLAVA
                     AFGDGTIRWYRLSDGKELLAFFPHKDRKRWVLWTSSGYYDASPGAEELIGWHLNRGKD
                     TAADFFPASRFRAAYYRPDVLARVLETGDEQEALRLANEESGRRRTEQAVASILPPVV
                     TILSPADGTAVSQTEVTLSVSLRSPSDAPVTAIRTLVDGRPVSTERGLVIKPTGDTRQ
                     IRVSIPPRDAEVSVIAENRNAASEPATIRLYWRGKATQDEFVIKPKLYVLAVGVSAYE
                     EADLQLGFAAKDARDFARALTGQKGGLYREVTARILTDKQATRDDIMDGLDWLQKETT
                     SKDIAMVFLAGHGVNDPAGIYYYLPVNANPEKLKRTGVAFSDIKNTMASLAGKAILFV
                     DTCHSGNVMGARRGTADINAVVNELASAENGAVVFASSTGRQYSLEDKAWGNGAFTKA
                     LVEGIGGRADYHGKGKITINMLDLYLSERVKELTGGKQTPTTTKPQTIPDFPIAVKR"
     gene            3796..4758
                     /gene="trxB"
                     /locus_tag="PLA58_07570"
     CDS             3796..4758
                     /gene="trxB"
                     /locus_tag="PLA58_07570"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOR62690.1"
                     /translation="MDHANLPTTDYDMIIIGGGPAGYTAGIYAARAALKTLLVEGAGT
                     VSQITITDMIENYPGIPEGIGGFELMQLFKKQAQKFGLEILPKDVTGVRKVSDSPVVW
                     EVAAGDRAYKTLSIIAATGAMWRSLGVPGEAQFAGKGVSYCATCDGPFYRNRTVVVVG
                     GGDTAIQEALFLTHFASKVFVIHRRDRLRAAGMLQNRAFAEKKIEFVWNATVEEISGA
                     EFVTGVKVKDVRTGKISEIAADGAFIFVGRLPYTDMFKGLVAMDKTGFIETDENLRTD
                     APGIFAAGDCRAKLFRQVVTAAGDGANAVHSAELYLDEVKGQVY"
     gene            4836..5681
                     /gene="lgt"
                     /locus_tag="PLA58_07575"
     CDS             4836..5681
                     /gene="lgt"
                     /locus_tag="PLA58_07575"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOR62691.1"
                     /translation="MESFLALWQHLPSHISPVLFSIGSFQLRYYSLMYLVAFAVVYAL
                     FAYRIKRGEIAFTTEGMQDYMVWAMLGLMIGGRLGYALFYNFSYYLSHPLEIFLPFDF
                     SNGVRFVGLSGMSYHGGLIGVLLVTIYFCRRRNIFWWRFGDWLCAAAPLGYLFGRIGN
                     FLNGELWGRATTVPWGMFFPQDATGSLRHPSQLYEALFEGLFLFMVLWGVRKRNPFDG
                     FMVGLYIFGYGLVRFVIEFFREPDAHLGFVLGFNTMGQVLCILMMLAGIAIILWRRRV
                     SAATK"
     gene            5696..5827
                     /locus_tag="PLA58_07580"
     CDS             5696..5827
                     /locus_tag="PLA58_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62692.1"
                     /translation="MGRNLVIVGGGAGGGATAAEAKRTDPSLNVTMLEQGKFVAFAA"
     gene            5831..7027
                     /locus_tag="PLA58_07585"
     CDS             5831..7027
                     /locus_tag="PLA58_07585"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR62693.1"
                     /translation="MPYYIGDVIQDEKKLIARTPEKFRESGIDAQIHARVEGIDPAKG
                     EVALRDGRIFPYDVLVMATGTSAFVPDLPGLDLPGVVSLRNLEDAIAIKTRLKEKNAK
                     RVVAIGGGFIGLEMSEALSAAGIATTIIHKDPLPANRWDAALSKVMLEELKANGVEFV
                     GGASARAIEKGKSAPLRVLTDRGDYEGDLVLLAIGVRPDAGLARATGLKIGSTGAIAV
                     NFSQQTSIANIYAAGDCCESFHRVSRRWVNVPLGDIANKQGRVAGRNIGGKPMTFDGI
                     VGAQSFRLFSLQCAATGLSEKEAVDAGFSPVSNITWGSAMAPALGMRKIGLKLTADRS
                     TGKLLGAQAVGAAGAVGRINALSVALWAGMDLDQIGYLDLAYSPPYSPSWDIIHNAAQ
                     ALGRLL"
     gene            7046..7984
                     /locus_tag="PLA58_07590"
     CDS             7046..7984
                     /locus_tag="PLA58_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOR62694.1"
                     /translation="MTVWWIFFGVFILAMLALDLGVFNRKTHVIQMKEALLWTSFWVT
                     LALSFGAGIYFFYGHGKAIEYVTAYLIEYSLSIDNLFVFMLIFRYFDVPRAYEHKALF
                     WGILLALITRGVFIFAGVALISRFHWVMYIFGAFLIVTGIKMALNKQTEVHPDKNIAI
                     RLLRYVMPVSKNFSGAKFFVVEGGVRVATPMLAVLLALETTDILFAIDSIPAVLAISQ
                     DPFIVYTSNVFAILGLRSLFFSISGLMRLFHLLHYGLAAILSFVGVKMMIADFFHVPV
                     GISLLIIASILAASIISSVVWPEKEEGEVPPVTMKE"
     gene            7992..8798
                     /locus_tag="PLA58_07595"
     CDS             7992..8798
                     /locus_tag="PLA58_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOR62695.1"
                     /translation="MLSNIPRASQNQLKRWASLQNTKFRREEGLFLAEGVKVVEELLK
                     SDRQAEALLVLPEKISVWEKIFRETKSNVPTYQLTRPEWKKLSQDREPEGIIAVVKAG
                     KEPSASSFLQSSGSHILILHEINNPGNLGALIRSACWFGFSGILLSANSVDWTNPKAV
                     RASMGGVFFLRILTNIDIAALLPEIQREYILVGSDPHRGVAPRALTKKAALLLGSESH
                     GLPDHLLGEVHERWRIPGGGGAESLSLPQAAAIMMYKMVDGGWCPSTGSG"
     assembly_gap    8833..8932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8933..9283
                     /gene="cutA"
                     /locus_tag="PLA58_07600"
     CDS             <8933..9283
                     /gene="cutA"
                     /locus_tag="PLA58_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOR62696.1"
                     /translation="GERREAMGDGEMTGFIQVMVTTETKEQARGIAQSLVGEKLAACV
                     QIVDSVESTYRWKGKVESARECLCLIKTRKSLFPKVEAAVKALHSYETPEIMAVPVVD
                     GSEEYLRWLDESLA"
     gene            9351..>11328
                     /gene="tkt"
                     /locus_tag="PLA58_07605"
     CDS             9351..>11328
                     /gene="tkt"
                     /locus_tag="PLA58_07605"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOR62697.1"
                     /translation="MPDKNELEKKCIDTIRFLAADAVEKAKSGHPGMALGAAPMAFAL
                     WTKHLKHNPENPDWPDRDRFILSAGHASMLLYALLHLTGYDLSLDDIKNFRQWGSRTP
                     GHPEYRHVPGVEVTTGPLGQGLANGVGMAVAEAHLAARFNRAGQQIIDHYTYVMASDG
                     DLMEGVAAESCSLAGHLRLGKLIVLYDDNRVSLAGSTGLAFTEDIEMRFKAYGWQVQK
                     VKDGNDVAAIDRAIKKAKKEKEKPSLICVRTTIGYGAPNKQGLCDSHGSPLGEKELNG
                     AKEACSWPTEQAFYVSGEVAKFFRRAVSHGRKYERAWQDALADYRRANPALAAELERA
                     LSGELPEGWDAALAEFPAGSKDMATRKASEAVMQMIASQVPQLVGGSADLNPSTCTWL
                     KGMGDFQSPNLGREGLHGMVGGAWGYSGRNIHFGVREHAMGAISVGLSLHGGVIPYTA
                     TFLTFADYMRPPMRLAALMGLRVIFVFTHDSVGVGEDGPTHQPVEQIMNLRQIPNMTV
                     IRPADANETLEAWRLALANTTGPTSLIFSRQNLPLLDRSICAPASCAGRGGYVLWESS
                     VLEPNIILMATGSEVALALEAGRRLAQEGTRVRVVSLPSWEIFDRQPQEYRDSVLPPR
                     IRARLAVEAGIKLGWEHYVGLSGKIIGMETFGASA"
BASE COUNT         2254 a         3147 c         3382 g         2445 t
ORIGIN      
        1 cataatggcc gactaaccct ttccggacgg ggttgttgaa aatatacttc aacacctcct
       61 gaagatcctc atcccggcgc aaaacgcggt catagtaacc gggctgccac aattggcgcg
      121 ccgccgtcgt ttcgctgcgg tatcgatatc cggttttctg cttgaaccct ctgacgaaag
      181 cttgcatgtc tgcatcttct ctctcccctt ccaggagaag atgcagatga tccggcatga
      241 aacaatagcc ccaaacggtg aaaccatgct cgtgggactt ttcggcaagg acggcaatgg
      301 ccgcgttgac ccgttcggaa tcttcgagca ggggttgttt ctggtccgtg catatcgtta
      361 taaagtagcg atggcggcca aggtagtcga agttcggcaa tctgggtttg cgtttgtacg
      421 gcatagaccg gcgctccggt ttgttataga aaaaatgtag tgcgggtctt tagacccgcc
      481 aaaaccagcg cggctaaagc cacgcactac attttttagc ggacctgaag gtccgcacta
      541 catcataaat cctacctctt caccgcaatc ggaaaatcgg gaatggtctg cggtttggtg
      601 gtggtcggcg tctgtttgcc gcccgtcaat tccttcaccc gctcggacaa atacaaatcc
      661 agcatgttga tcgtgatttt tcctttgcca tgataatccg cccggccgcc gatgccttcc
      721 accagcgctt tggtgaacgc gccattgccc cacgccttgt cttccagcga atattgcctg
      781 cccgtggaag aggcaaaaac caccgcgccg ttttccgcgc tggccagctc attgaccact
      841 gcgttaatat ccgccgtccc gcgtcttgct cccatcacat tgccggaatg gcacgtatcc
      901 acaaacagaa tcgccttccc cgccagcgac gccatcgtgt ttttgatgtc ggaaaaagcc
      961 acgcccgttc ttttcagctt ttccggattg gcgttcaccg gcagataata gtaaatcccc
     1021 gccgggtcgt tgacgccgtg gccggccaga aacaccatcg caatatcctt gctggtcgtc
     1081 tccttttgca gccagtccag tccgtccatg atgtcatcgc gtgtggcctg tttatcggtt
     1141 aaaatccgcg cggtcacctc gcggtaaagg ccgcccttct gacctgtcag cgcccgggca
     1201 aagtcgcggg catccttggc ggcaaaacca agctgcaaat ccgcttcttc ataggcgctg
     1261 acccccacgg ccagaacgta aagtttcggc ttgatgacaa actcatcctg cgtcgccttg
     1321 cctcgccaat agaggcgaat tgtggccggt tcgctcgcgg cattgcggtt ttccgcgata
     1381 accgagactt ccgcatcccg gggcggaatt gaaaccctga tttgccttgt atctcccgtg
     1441 ggtttgataa ccagaccacg ctccgtggat accggccggc catccaccag tgtccggatg
     1501 gccgtcaccg gcgcatctga cgggctgcgc agggaaacgg aaagagtcac ctccgtttgc
     1561 gagacagccg tgccatcggc aggcgagagg attgtcacta ccgggggcag aatgctggca
     1621 acggcctgtt ctgtccttct gcggcctgat tcttcattgg ccagacgcag cgcttcctgt
     1681 tcatcacctg tttccagaac tctggcaagc acatcagggc ggtaataggc ggcgcgaaag
     1741 cgggaggcgg gaaagaaatc cgccgccgtg tccttgccgc gattgagatg ccagccaatc
     1801 agttcttcag cgccgggaga ggcgtcgtag tagccggatg atgtccagag tacccagcgc
     1861 ttcctgtctt tgtgggggaa gaaggccagc agctccttcc cgtcggagag gcgataccag
     1921 cggattgtac cgtcgccaaa ggcggccacg gccaggcggc cgtctcggga gatgttcacc
     1981 gcccaggtta cgccgggaga gggggcctgc catttttcat tgccgctcct gtcgaaatag
     2041 cgcagtgacc aatctgctcc cagcagaaag gcgtcccggg acggcgaaat ggcgacactg
     2101 cgggagattt cgtattgctt cagcgccagc gcccggccgt ttagtttggg ggaggttgca
     2161 tgtttccagt ccgtgacgga aagactattt gtatcagggg gatacaggtc ggatgtatcc
     2221 ccgtcgggag acagcatgag tgtggacagg tcaaacgtgg cgggggagtt gccaccgtat
     2281 tcatacccga agcttacctt tttgccgtcg gacgagaggc gaaaaccttc acgattaccc
     2341 cggtaatccg caatctgcgc gctttgaaaa aggacgcgcc ggccgttttt atccagaacg
     2401 ccaaaggcgg ggtctcccga accataggcc acgccgcccg tggccagcgg catgaagtgc
     2461 atgatggtat taagcgcacc gggcagctcc cggcgctggc cccggccctc tttttcccaa
     2521 gaaagaatga gggtttggcc gtttttgtaa tatgttcctc ccgcatacag gcgaccgtcg
     2581 ggggaaaagg agacgcgccc gagatttccg ttatccacac cggaggtgtc cggggaataa
     2641 aggtgggaga ggtctgaggc ggaaaggaca tccaccgccg tggaatcgtt aaagcccacg
     2701 gccagctttt ccccgtcggg ggaaaaggaa agcgaaaatg gctgcttgtt gtcgggagaa
     2761 aaggaaagga aaaatgaccg cttccccccc ggggcctttt gtttagccag aagcttcagg
     2821 ccatccgcgc ccggacggtc gtagaggcgg atataaccgt cgaaagaggc tgcagccagg
     2881 cgaccccggc ggtcaaaaac gacgtagtag gaatcagcgc cgtaatccct gtcctcgccc
     2941 accagcgccc cgtcggcggt gcggaaaacg cggatgccgt tatttttccc caacgtcgct
     3001 gccagataga gcccgtcggg agaaaaggaa agatgaaaga tgacgttggg gaggccggta
     3061 atccggcggg tcagtttccc cgtggcgcgg tggaaaaggt agatgctcac accatcatcg
     3121 gcaagtccgc cttccggtcc ggcaaattgt gtccaccccc cacaggccac ggtttgcccg
     3181 tcgggagaaa tcgccactgc atcaattctg ccttcatttc cctcgccaat tggcggccgc
     3241 aggacggaga gcagtcggcc ggtggccagt tcccacaccc ggacgctctt gtcgtgggag
     3301 ccggtcacca gataccggtt ggcgtcatcc acgctgatgt ttctgatgaa cgccgtgtgc
     3361 atgccagttt cgattttcag aatcggctct ttgggcggct cggctgcccc ggcaatcccc
     3421 gaaaaaacaa tccccacgac cagcgtgcaa aataatcgtt tcatcattgc ctccatttcc
     3481 cgtgtagtgc gggtctttag acccgccaat agcggacctg aaggtccgca ctacattttc
     3541 attcaaaaca gcgaggctaa agccacgcac tacatttaat attttagcgg acataaatgt
     3601 ccgcactaca ttgacgttat tcattactgg tatttatagg aaacgtgggt ttgtatgtca
     3661 agaaaatcat ttcggaaaag cctgtcgggc tttaatttcc gcgccgtgtt attctgtcgc
     3721 cacccgtcaa tttggccttt acaaaagcgc ctcttcctga ttaaagtcag tcatcaaatc
     3781 ttcaggagat ttttcatgga tcacgcaaac ctgccgacaa cggactacga tatgatcatt
     3841 atcggcggag gtcccgccgg ctacacggcc ggcatctacg ccgcgcgtgc cgctctgaaa
     3901 actctgctgg tcgaaggtgc gggaacggtc agccagatca cgatcacgga catgatcgaa
     3961 aattatccgg gcatccccga aggcatcggc ggcttcgagc tgatgcagct ctttaaaaag
     4021 caggcgcaga agttcggcct ggaaatcctg cccaaagacg tcaccggcgt gagaaaagtt
     4081 tccgattcgc cggtggtctg ggaagtggcg gcaggtgacc gggcttacaa aacattgagc
     4141 atcattgctg cgacgggtgc gatgtggcgg agcctgggcg ttccgggtga ggcgcaattc
     4201 gcggggaagg gcgtttcgta ctgcgccacc tgcgacgggc cgttttaccg caaccgcacg
     4261 gtggttgtgg tgggcggcgg cgataccgcc atccaggaag cgctgtttct gacgcatttt
     4321 gcctccaaag tcttcgtgat tcaccggcgc gatcgtctgc gcgcggcggg catgctgcag
     4381 aaccgggcct tcgccgaaaa gaagatcgag ttcgtctgga acgcgaccgt tgaggaaatt
     4441 tccggcgccg aattcgtcac gggcgtgaaa gtgaaagatg tccggacggg aaagatcagt
     4501 gaaatcgcag ccgacggcgc ttttattttt gtcggccgcc tgccctacac ggacatgttc
     4561 aaagggctgg tggcgatgga caaaacgggc ttcattgaga cggatgaaaa tctgcgcacc
     4621 gatgcccccg gcattttcgc cgcgggagat tgccgcgcca agctgttcag gcaggtggtc
     4681 accgccgcgg gcgacggcgc caacgccgtg cacagcgcgg agctgtacct ggatgaagtc
     4741 aagggacagg tgtactgacc ggcgttgtca gcctttctct gccgtcttac aacgcgtttc
     4801 ggccggattt atattctttt aaaaatggaa acctgatgga atcttttctc gctctctggc
     4861 agcatctgcc ctcgcacatc agccccgtgc ttttcagcat cggctccttt cagcttcgct
     4921 attacagcct catgtatctg gtggctttcg ccgtggttta cgctctgttt gcctaccgga
     4981 tcaagcgcgg ggaaattgcc ttcacgacgg aaggcatgca ggattacatg gtctgggcga
     5041 tgctcggcct gatgatcggc gggcggctgg gttatgcgct gttttataat ttctcctact
     5101 atctttccca tccgctggaa attttcctgc cgttcgactt ctccaacggg gtccgctttg
     5161 tgggattgag cggcatgtcc taccacggcg gcctcatcgg cgtcttgctg gtcacgattt
     5221 atttttgccg ccggcgcaac atcttctggt ggcgcttcgg cgactggctt tgcgcggcgg
     5281 cgccgctggg gtatctgttc ggccggatcg gcaattttct caacggcgaa ctctggggcc
     5341 gggcgacgac cgtgccgtgg ggcatgtttt ttccgcagga cgcgacgggg agcctgcggc
     5401 atccgtccca gctttacgaa gccctgttcg aggggctgtt tttattcatg gtcctgtggg
     5461 gcgtccgcaa gcgcaatccc tttgacggct ttatggtcgg cctgtatatt ttcggctacg
     5521 gcctggtgcg cttcgtcatc gagtttttcc gggagccgga cgcgcatctg ggatttgttc
     5581 tgggattcaa caccatgggg caggtgttgt gcattctgat gatgctggcg ggaatcgcga
     5641 ttattctctg gcgcaggcgg gtatccgccg cgacaaaata agccgggagg ttctgatggg
     5701 caggaatctg gtgattgttg gcggaggcgc cggcgggggg gccacggcgg cggaagcaaa
     5761 aaggaccgat ccgtcgctga acgtcacgat gctggagcag gggaagtttg tcgcctttgc
     5821 cgcctgaccc atgccctatt acatcgggga tgtaattcag gacgaaaaga aactgatcgc
     5881 gcgcacgccg gaaaaattcc gcgaaagcgg cattgacgcg cagatccatg cgcgcgtgga
     5941 aggcatcgat ccggcaaagg gcgaagtcgc gctccgggac gggcggattt ttccctatga
     6001 cgttctggtg atggccaccg ggacctccgc ttttgtgccg gatctgccgg ggcttgacct
     6061 gccgggcgtg gtttccctgc gcaatctgga ggacgccatc gccatcaaga cccggctcaa
     6121 agaaaaaaat gcgaagcggg tcgtcgcgat cggcggcggc ttcatcggtc tggaaatgag
     6181 cgaggcgctt tcggccgcgg gcattgccac aaccatcatc cacaaggatc cgcttccggc
     6241 caaccgctgg gacgcggcgc tttcgaaagt catgctggag gagcttaagg ctaacggcgt
     6301 ggaatttgtc ggaggcgcgt ccgcccgggc cattgaaaag gggaaaagcg cgccgctgcg
     6361 cgtcctgacg gatcggggag attatgaagg cgatctggtt ttgctggcca tcggtgtccg
     6421 gcccgatgcg ggcctggccc gggcgacggg tttgaaaata ggttcaaccg gagcgattgc
     6481 cgtcaatttt tcccagcaga cctccatcgc caacatttac gcggcgggcg actgctgcga
     6541 atcctttcat cgcgtcagcc gccgctgggt aaatgttccc ctgggcgaca tcgccaacaa
     6601 gcagggccgc gtggccgggc gcaatatcgg aggcaaaccg atgacgtttg acggcattgt
     6661 cggcgctcag tctttccggc tgttttcact ccaatgcgcc gcgacggggc tgtcggaaaa
     6721 ggaagccgtc gacgcgggct tttctccggt gagcaacatc acctggggca gcgccatggc
     6781 gcccgcgctg ggcatgagaa aaatcggcct caagctgacg gccgaccgtt cgacgggcaa
     6841 actgctgggc gcacaggcgg taggggccgc cggcgcggta ggcaggatca acgcgctttc
     6901 cgtcgcgctc tgggcgggga tggacctgga tcagatcggc tacctggacc tggcctactc
     6961 gccgccgtac agtccgtcct gggacattat tcataacgcg gcccaggcgc tcggccgttt
     7021 attgtaagca gaggaatcgg atgtcatgac cgtttggtgg atcttctttg gggtgttcat
     7081 tctggcgatg ctggcgctgg atctcggcgt cttcaaccgg aaaacccacg tcatccaaat
     7141 gaaggaagcg ctgctctgga cgtccttctg ggtgacgctg gctttgtcgt tcggggcggg
     7201 gatttatttc ttttacggac acggaaaagc catcgagtat gtgaccgctt acctgattga
     7261 atactcgctc agcatcgaca atctctttgt cttcatgctg atctttcgct acttcgacgt
     7321 tccccgcgct tacgaacaca aggcgctgtt ctgggggatt cttctggcgc tgatcacgcg
     7381 cggcgttttc atcttcgcgg gcgtggcgct gatcagccgc ttccactggg tcatgtatat
     7441 tttcggcgcc tttctgattg tcaccggcat caaaatggcg ctgaacaagc agacggaagt
     7501 ccaccccgac aaaaacatcg cgatccggct tttgcgctac gtcatgccgg tttcgaaaaa
     7561 cttctccgga gcgaaatttt ttgttgtcga agggggtgtg cgggttgcca cgccgatgct
     7621 ggccgttttg ctcgccctgg agacgaccga cattcttttc gcgatcgact cgattcccgc
     7681 cgtgctggcc atttcccagg atccctttat cgtttacacc tccaacgtct tcgcgattct
     7741 gggcctgcgg tcgctgtttt tttccatttc cggcctgatg cggctgttcc accttcttca
     7801 ttacggtctg gcggccatcc tgtcctttgt cggcgtcaag atgatgatcg cggatttttt
     7861 ccatgtgccg gtcgggattt cgcttttgat tattgcttcg attctggctg cttcgatcat
     7921 ttcatcggtt gtctggccgg aaaaagagga aggcgaggtt cccccggtaa ccatgaagga
     7981 gtgagggtga catgctgtcc aacattcccc gcgcgtctca aaatcagttg aaaagatggg
     8041 caagcctgca aaacacgaag tttcgccgcg aggaaggcct gtttctggcc gaaggagtga
     8101 aagttgttga agaactgctg aaaagcgacc ggcaggccga agccctgctc gttctgccgg
     8161 aaaaaatttc cgtctgggag aaaattttcc gtgagacaaa gagcaacgtt cccacttatc
     8221 agttgacacg cccggagtgg aaaaaactga gccaggacag ggagccggag ggaatcatcg
     8281 ctgtcgtcaa agccggaaag gagccttccg cttcttcttt tctccaatcg tccggcagcc
     8341 atattttgat ccttcatgaa atcaacaatc ccggcaactt gggcgcgctg atccgcagcg
     8401 cctgctggtt cggcttttcc ggtattctcc tcagcgccaa ctcggtggac tggaccaatc
     8461 ccaaggcggt ccgggcgtcg atgggcggcg tttttttcct gagaattttg acgaatatcg
     8521 acattgcggc cctgctgccg gaaatccagc gggagtacat tcttgtcggc agcgacccgc
     8581 accggggcgt cgcgccgcgc gcgctgacga aaaaagcggc gctgcttctg gggagcgaaa
     8641 gccacggcct gccggatcat ctgcttggcg aggtccatga gcgctggcga atccccggcg
     8701 ggggaggcgc cgaatcgctc agtcttccgc aggcggcagc gattatgatg tataaaatgg
     8761 tggatggggg atggtgcccc tcgacgggct cggggtgaca cagacagtga tgagtgaaat
     8821 gtagggaacg gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcgagag
     8941 gcgagaggca atgggcgatg gggagatgac gggatttatt caggtgatgg tgacaacgga
     9001 aacgaaggag caggccagag gcatcgcaca gagcctggtc ggggaaaaac tggccgcctg
     9061 cgtccagatt gtcgattccg tcgaaagcac gtaccgctgg aagggcaagg tggagagcgc
     9121 ccgggaatgc ctgtgcctga tcaaaacccg caaaagcctg tttccaaaag tcgaagccgc
     9181 cgttaaggcg ctgcattctt acgagacccc tgaaatcatg gcggttcctg ttgtcgacgg
     9241 cagcgaggaa tatctgcggt ggctcgatga atcgctggct taaattttac gtgcaataat
     9301 ttcaggtttg ttttaaggga agcaccgtga aaaaacatga aggagacttt atgccggaca
     9361 aaaacgaact ggaaaagaaa tgcattgata ccataaggtt tttggcggcg gacgcggttg
     9421 aaaaggccaa atccggccac ccgggcatgg cgcttggcgc ggcgccaatg gcctttgcgt
     9481 tgtggaccaa acatttaaag cataatcctg aaaatccaga ctggccggac cgggaccggt
     9541 ttattctgtc ggccggtcac gcctcgatgc tgttgtacgc gcttctgcac ctgacaggct
     9601 atgatttatc tctggatgac attaaaaatt tccgccagtg gggaagccgg acgccggggc
     9661 accccgagta caggcatgtg ccgggtgttg aagttaccac cggccccctg ggccagggac
     9721 tggccaacgg tgtcggcatg gccgttgccg aagcgcacct ggctgcccgt ttcaaccgcg
     9781 cgggtcagca gatcatcgat cattatacgt atgtcatggc cagcgacggt gatttgatgg
     9841 aaggtgtggc cgcggaatcc tgctctctgg ctggccatct gcgcctggga aagctgatcg
     9901 tcctgtatga cgacaaccgg gtctctctgg caggttccac gggtcttgcc tttaccgagg
     9961 acatcgagat gcgcttcaag gcttacggct ggcaggtgca gaaggtcaaa gacggaaacg
    10021 atgtggccgc tatcgaccgg gccattaaaa aagccaaaaa agaaaaggaa aaaccgtcgc
    10081 tcatttgcgt gcggaccacg atcggctacg gcgccccaaa caaacagggt ttgtgcgatt
    10141 cgcacggctc tcccctgggg gaaaaggagc tcaacggcgc caaagaagcc tgctcctggc
    10201 ccacggagca ggcattttat gtttccgggg aggtcgcgaa atttttccgc agggcggttt
    10261 cccacgggag aaagtacgag cgcgcctggc aggatgcgct ggccgactac cggcgggcga
    10321 atccggccct ggccgctgaa ctggaaagag ctctttcggg cgaactgccc gagggctggg
    10381 acgcggcgct tgccgaattc ccggcggggt ccaaggatat ggccacgcgc aaagcttcgg
    10441 aagccgtgat gcaaatgatc gcctcccagg taccccagct ggtcggaggg tcggcggacc
    10501 tgaatccctc gacctgcacc tggctcaagg gtatgggtga ttttcaaagt ccgaatctcg
    10561 gcagggaagg tctgcacggc atggtgggcg gcgcctgggg ttactcgggg cgcaatattc
    10621 atttcggcgt gcgcgaacat gcgatgggcg ccatctcggt ggggctgtcg ctgcacggag
    10681 gcgtgatccc ctacacggca acctttttga cattcgcgga ctacatgcgc ccgcccatgc
    10741 ggctggctgc tttaatgggt cttcgcgtca tttttgtctt tacgcatgac agcgtcggcg
    10801 tgggcgaaga cggtccgacg catcagccgg tcgagcagat catgaatctt cgtcagattc
    10861 ccaacatgac ggttatccgg ccggcggacg ccaacgaaac gctggaggcc tggcgtctgg
    10921 cgctggccaa caccaccggg ccgacgtcgc tgatcttcag ccgacagaac ctgccgcttc
    10981 tggatcgctc catctgtgcg cccgcctcct gcgcgggccg gggcggctat gtcctgtggg
    11041 agtcgtctgt cctcgagccg aacattatcc tgatggccac cggttcggaa gtggccctgg
    11101 cgctggaagc cggccgcagg ctggcgcagg aaggaacccg cgtccgtgtt gtgtcgctgc
    11161 ccagctggga aatttttgac cgccagccgc aggaataccg cgattcggtc ctgccgccga
    11221 gaattcgggc gcgcctggcc gtggaagcgg gaatcaagct gggatgggag cactatgtcg
    11281 ggctttcggg aaagatcatc ggcatggaga ccttcggcgc gtccgcgc
//
LOCUS       DAOSRM010000073        11291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5669_length_11291_cov_98.728729, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000073 DAOSRM010000000
VERSION     DAOSRM010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5669_length_11291_cov_98.728729"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /gene="rnpB"
                     /locus_tag="PLA58_07610"
     ncRNA           complement(<1..213)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLA58_07610"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(331..744)
                     /locus_tag="PLA58_07615"
     CDS             complement(331..744)
                     /locus_tag="PLA58_07615"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOR62698.1"
                     /translation="MEQKPVKLFSDGACRGNPGPGGGGAVITDASDRILWEGKEYFGR
                     CTNNIAEYKALILGLKGALAGGWRNVEIYMDSELLANQINGSYKVKNENLKILMQDVR
                     KLLSSLESARVRHVPRCRNATADRLANLAIDEQGK"
     gene            complement(751..1470)
                     /locus_tag="PLA58_07620"
     CDS             complement(751..1470)
                     /locus_tag="PLA58_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOR62699.1"
                     /translation="MKEQLLLLIGLQECDSQLVKLAAKKTRLPEEIQAMENDFQAYRE
                     SIEQNKQKQEDLKARRAEYEAAIKKIGDGMIKTKERLLEVKNNKEYQAMLKEIETAEK
                     TLGEMETRIIALMEDMDKLSVLVKQDDAVLAQKTRKHEQEKKIIEDDLSAVDADTACW
                     TEKRAELQKQIPADLLNRYERIKKRNNGVGVISVWKAVCSGCHMNIPPQLYNELQRSN
                     ELLSCPNCNRIMYFKNQEKAE"
     gene            complement(1710..1785)
                     /locus_tag="PLA58_07625"
     tRNA            complement(1710..1785)
                     /locus_tag="PLA58_07625"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1750..1752),aa:Ile,seq:cat)
     gene            complement(1866..3614)
                     /gene="rpoD"
                     /locus_tag="PLA58_07630"
     CDS             complement(1866..3614)
                     /gene="rpoD"
                     /locus_tag="PLA58_07630"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOR62700.1"
                     /translation="MAKEIQSDEFKKLLSLGEEKGFLTYDDVNDMLPPDLNSSEQIDD
                     IIMLFGEKNIDIIDMEQGEKLVVKKPVEDALPSKFPEALALVPGAGKTGDPVKMYLRE
                     MGLVSLLSREGEVEIAKKIEEGARETMWAIFSLTVSIDEVLSIGEKLESGEIRVKNVV
                     DNIEDEEGFMEEDDHKERVLRLIARIRLFNDRNKVLREKLKSKTMRAKQRETLTAELV
                     KNTKNIITLCRKIRFSKKQMHRFIARLKSFTDEIERSERVINHFKKESGLNISQMEKA
                     WSRMRKSKQDERQAAKEAKVSIDWLHRSHSAVTEAQKRLKHIAREVGIPTATLKTVVY
                     SIEDGEAKAEIAKRKLVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEY
                     QRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRYLVQELGREPT
                     PEEIANKMEYPLEKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKKIMSPSEATIS
                     MDLADQTRKILSTLTPREEKVLRMRFGISDRLGTTVLGEATLEETVVSEVEEPQVVEE
                     EPLKKAKHSPRKRTGK"
     gene            complement(3632..5404)
                     /gene="dnaG"
                     /locus_tag="PLA58_07635"
     CDS             complement(3632..5404)
                     /gene="dnaG"
                     /locus_tag="PLA58_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOR62701.1"
                     /translation="MRFDDSKIEEIKSRVDIVELASEYLTLKKAGRNYLGLCPFHQEK
                     TPSFTVNREKQIFYCFGCGEGGNVITLLMKIANKSFPEAVKYLAEKTGVLLPVRTFGS
                     DGREKESLRDGMFQLNARAARYFARQLSSPAGKAARDYLQKRAVTEETVRQFRLGYAP
                     DAWRSLADDVEGGGLSLKMAEQAGLVIAGKEGGFYDRFRGRLMFPIENPFGEIVAFGG
                     RIIGEGEPKYLNSPESPVYSKGKNLYGLNKAKETIRRNGFCLIVEGYFDVISLWNAGV
                     RNVVATLGTALTRDHLELLRRYTQDVVALFDPDAAGRKALDRSLELFLGANMSARALI
                     LPDGLDPDDFVKKYGPAKLEELIALAPAISDYYIDSVLGNGKTFEENRDLVKTAMEFV
                     GKINNEIEKNLFIKRIAEKSGIDQALLKREIHRKPASAPSGAGVKKPSGGMAVNPLEF
                     NLIRLMLEYPQKALLVDDENILECFLEPALKDLGTKIIQTYKLLGYVDVNILLPSDED
                     KPLREHLYKMSVEAPPTDDDRVERNFVDNMRRIREKWYKEQKRQVQHKLRQAQECGDP
                     DLIRELTFQKQHLIKEEHKYLNQS"
     gene            complement(5460..5906)
                     /locus_tag="PLA58_07640"
     CDS             complement(5460..5906)
                     /locus_tag="PLA58_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOR62702.1"
                     /translation="MDILAKLNEDMVTAAKAKDKIRLSALRLLKTALHNKEIDLMRPL
                     NETETMQILSGLVKQRKDSIEQFAKGGRQDLVEKEEAELKILQDFMPAQMSDEEVEAL
                     IKKAIADVGALSVKDMGKVMKALMPQITGKADGKAAGEKVKALLSQ"
     gene            complement(5923..6264)
                     /locus_tag="PLA58_07645"
     CDS             complement(5923..6264)
                     /locus_tag="PLA58_07645"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOR62703.1"
                     /translation="MAECIFCKIIKGDIPSKKVYEDDAVLAFDDISPMAPVHVIIIPK
                     KHIATLLDVDTNADIMGRWMAAAQQVAKMKQVADSGFRLAINCNADGGQVVFHLHMHL
                     LGGRKLEDPLG"
     gene            complement(6286..7017)
                     /gene="hisA"
                     /locus_tag="PLA58_07650"
     CDS             complement(6286..7017)
                     /gene="hisA"
                     /locus_tag="PLA58_07650"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOR62704.1"
                     /translation="MLIIPAVDVKNGQCVRLAQGDFNRVTVYAENPVDMARRWVQKGA
                     QRIHLVDLDGSVAGLPKNAPILVEIAKSVPVPVEVGGGIRSMETVNYYLENGVSSVIL
                     GTSAIADEAFVRTAARTHRGKIILGIDALAGEVAVRGWTQKTGQNAVELAERYVHDGI
                     QAIVYTDISRDGMEAGVNVEQTRALAKAVNIPVIASGGVATLADIDALLAVKDCSFYG
                     VIVGRALYTGAIALEDAIAKTQAAS"
     gene            complement(7093..7779)
                     /locus_tag="PLA58_07655"
     CDS             complement(7093..7779)
                     /locus_tag="PLA58_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62705.1"
                     /translation="MSHNRFSIKSIGCALWLLGFFLLSQGVGHAQEEPKAAEKKATRI
                     AVIPFQAVLPEEPSSTVQCPICGSVNSSGGISKGAERMVEEMVTDKLMDYKNVEIIPR
                     EKVAGVYRWVSTALRKQPLLQTFRETGSELGADVLVAGFVYRYRERLGYDYSVERPAA
                     VAFEIHLIAVGDGRILWRGIFDKTQKSLMEDVFQASSFFKGGAKWLTARQLTKLGVDD
                     IFKTFPGFER"
     gene            complement(7799..8386)
                     /gene="hisB"
                     /locus_tag="PLA58_07660"
     CDS             complement(7799..8386)
                     /gene="hisB"
                     /locus_tag="PLA58_07660"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOR62706.1"
                     /translation="MARKAKIVRKTTETDIALDIDLDKSAGSRIDTTIPFLNHMLELF
                     ARHGRFKLVVKSKGDTEIDDHHLVEDLGICLGEAVRQALGTKTGINRYGSASVPMDES
                     LCRVDMDICGRPYLIYKVRYERRKIGGFDPALVKEFFKAFTDHSGITLHITLEYGSNS
                     HHIIEAVFKAFARALRQAVNIHPEISGVLSTKGTL"
     gene            complement(8402..9274)
                     /gene="hisG"
                     /locus_tag="PLA58_07665"
     CDS             complement(8402..9274)
                     /gene="hisG"
                     /locus_tag="PLA58_07665"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOR62707.1"
                     /translation="MNVLKLGIPKGSLEKNTVDLFQKAGWRITYDSRNYFPDIDDPEI
                     SCTLVRAQEMSRYIEDGHLDLGLTGIDWILENNSDVQVVQDLIYSKVSTRQARWVLVV
                     RDDSEIRSIEDMEGRHISTELVNFTRRYFAERKIKVGVEFSWGATEAKVVDGLVDAVV
                     EVTETGSTIRANKLRIVHELMKTNTQLIANRAAWNDPWKRKKIEQIRTLLTGSLLAMG
                     KVGLKLNVAKENLDRVMKLLPSLKAPTISTLYSEEWFAIETVIDTGAVRDIIPRLLDA
                     GAQGIIEYPLNKVI"
     gene            complement(9271..9645)
                     /gene="hisI"
                     /locus_tag="PLA58_07670"
     CDS             complement(9271..9645)
                     /gene="hisI"
                     /locus_tag="PLA58_07670"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOR62708.1"
                     /translation="MIEPNFNKTDGLIPAIVQDAETKDVLMLAYMNRASWEATLQSGK
                     ATFWSRSRQKLWLKGESSGHVQIVKDIFIDCDEDTLLLQVEQLGGAACHTGHRTCFFR
                     RRKGGDFEVVGKKVFDPKEVYK"
     gene            9846..10142
                     /locus_tag="PLA58_07675"
     CDS             9846..10142
                     /locus_tag="PLA58_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62709.1"
                     /translation="MSLLLGGVVAAVIGLISLIFWWSDFLAILRGALPIFLFLGGALA
                     VYVGFDDIQDKIREERQRQNEELEKAREEVESAKAQAERYKDELDRLKDEKKQS"
     gene            10516..>11291
                     /locus_tag="PLA58_07680"
     CDS             10516..>11291
                     /locus_tag="PLA58_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOR62710.1"
                     /translation="MEKKGFFVTISLLCAIFFVLVFSSGVLAQTTLTYSIFFPATHAQ
                     AKAGDAWAKEIEKRTGGKVKINIFAGGSLTPADQCFDGVVKGISDIGMSCFAYTRGRL
                     PLLEVLDLPLGYPNGMVATLVANDLVKKMKPKELDDVKVLYLHAHGPGLLHTQKPVYK
                     MEHLKGMKVRSTGLSAKIVENLGGISVAMPQGGTYEALQKGVVQGTLAPMETLKGWKQ
                     AQVVKYTTDCTNIGYTTAMFVVMNKKKWESLPKDVQKVFD"
BASE COUNT         2361 a         3203 c         2761 g         2966 t
ORIGIN      
        1 tggggtttac catgcctttt ccgtcaccgg aaaagcggtg agctcttacc tcgccatttc
       61 acccttaccc tgaggctttc gcccgggcgg tatattttct gtggcacttt ccctggggtc
      121 gcccccggtc gccgttagcg accatcctgc cctgtggagc tcggactttc ctccggcaga
      181 ggaaactctg cccgcgatca tccgattttc tccggcctgg gaagtatcct acaattgatt
      241 tggattttgt tcaaggacaa aggacacatg taagaagata ttcgacgcct tcagtctcgc
      301 gccccttttt ttttgggggg ggggcagtcc ttacttcccc tgctcgtcaa tggccaggtt
      361 ggccagccgg tcggctgtcg cattgcggca gcgcggaaca tgccggaccc tggcggattc
      421 aagggaagac agcagcttcc ggacgtcctg catcaatatt ttcaggttct cgttttttac
      481 tttgtaggaa ccgttgatct gattggccag gagctccgaa tccatataaa tttctacgtt
      541 tctccagccg ccggccagag cgcctttcag ccccaggatc agcgccttat attccgcgat
      601 attattggtg cagcgcccga agtattcctt cccctcccaa agaatccggt ccgaggcatc
      661 ggtaatcacc gccccgccgc ctcccggccc gggatttccc cggcaggctc cgtcgctgaa
      721 caacttgacc ggtttttgtt ccatagaaaa ctattcggcc ttttcctgat ttttaaaata
      781 cataatccgg ttgcagttcg ggcagctcag cagttcattg gagcgctgca attcgttgta
      841 gagctgcggc ggaatattca tgtggcagcc gctgcaaacc gctttccaga cggaaatcac
      901 accaacgccg ttattgcgct tttttattct ttcataccgg ttcagaaggt ccgcggggat
      961 ttgcttttgc aattcggccc gtttttccgt ccagcatgcc gtatcggcgt ccacggcgct
     1021 caagtcatct tcgatgattt tcttttcctg ttcgtgtttg cgggttttct gcgccaggac
     1081 tgcatcatcc tgcttgacca gcacggaaag tttgtccatg tcttccatca gggcgatgat
     1141 ccgggtttcc atctcaccga gggttttctc cgccgtttca atctccttga gcatggcctg
     1201 atattctttg ttgtttttga cttcaaggag cctttctttg gttttaatca tcccgtcgcc
     1261 gatctttttg atggcggctt catattccgc gcgccgggct ttgaggtctt cctgtttttg
     1321 cttgttttgc tcgatgcttt cccgataggc ctgaaaatca ttttccattg cctggatttc
     1381 ttccggcagc ctggtctttt tggcggcaag cttgaccaat tgagaatcac actcctgcaa
     1441 tccgatcaac agcagcaatt gttctttcac cccgttctcc ttctttttta ttttttcatc
     1501 ccctgatgct tccccgacgc caaaaaaaat gcaccttttg aggggtgcat tttcagatca
     1561 acgtcggatg gggctggttt tgggcgccgc gatagaataa tcgaacattt ctgatcgcgg
     1621 cttttgcctc taccgttttg tcgcagcaac tccttgcttg tgaaaatcgc ttttttccgg
     1681 ccatccgtaa agcttccttt cataaaattt ggtgggccca cctgggatcg aaccagggac
     1741 ctaccggtta tgagccggtg gctctaccaa ttgagctata ggcccgccat gttcagccat
     1801 tgcgcgaatg tttcaaccgg catcaaggcg cgttgaaata tacccaatcc cggcgcttgt
     1861 caatattatt ttcccgtccg tttccggggg gaatgctttg cttttttcaa cggctcctct
     1921 tcaacgacct ggggctcttc aacctcggag accaccgtct cctccagcgt cgcttcgccc
     1981 agaaccgtcg ttcccagccg gtcgctgatt ccgaaacgca ttcgcagaac tttttcttca
     2041 cgcggcgtca gcgtggacag tattttgcgc gtctggtccg ccagatccat gctgatggtg
     2101 gcctccgatg gagacatgat ctttttgtct tcgataaagt ctcccagatg gctgtcctcc
     2161 tcctcgccga tcggtgtttc cagggaaatc ggctctttgg caattttcag caccttgcgc
     2221 actttttcca gcggatattc cattttgttg gcgatttctt ccggggtcgg ttcacgtccc
     2281 aattcctgca cgaggtatcg cgatgtccgg atcagtttgt tgatcgtttc aatcatatgg
     2341 acgggtatcc gaatcgtgcg ggcctggtcg gcaatcgcgc gcgtaatggc ctggcgaatc
     2401 caccaggttg cgtaggtgga aaacttatag ccccgctgat attcgaattt atcaaccgcc
     2461 ttcatgagcc caatattgcc ttcctgaatc aaatccagaa attgcaggcc gcgattggtg
     2521 tactttttgg caatgctgac gaccagcctt aagttggctt cgaccagttt ccgcttggcg
     2581 atttccgcct tggcctctcc gtcttcgatg gaataaacaa ccgtcttgag cgtggcggtt
     2641 ggaatcccca cctccctggc aatatgcttc aatcgttttt gggcttcggt cacggccgag
     2701 tgactccggt gcagccaatc gatggacact tttgcctctt tcgccgcctg ccgctcgtcc
     2761 tgtttggatt tcctcatcct ggaccaggcc ttttccattt gcgaaatatt caggcccgac
     2821 tcttttttga aatggttgat cacacgttcc gagcgttcaa tctcgtccgt gaaggatttc
     2881 aaccgggcga taaaacggtg catctgcttt ttgctgaaac ggattttccg gcaaagggtg
     2941 atgatatttt ttgtgttttt caccagctcc gcggtcaggg tctcccgctg cttggccctc
     3001 atggttttgg atttaagctt ctcacgcagc accttattgc ggtcattgaa gagccgaatc
     3061 cgggcgatca gccgaagcac tctttccttg tgatcgtcct cttccatgaa cccttcttcg
     3121 tcctcgatgt tatccacgac atttttgacg cggatttctc cggattcaag cttctctccg
     3181 atgctgagca cttcgtcgat ggaaacggtc agactgaaaa tcgcccacat ggtttcccgg
     3241 gcgccctctt cgattttctt ggcgatctcg acttcacctt cgcggctcag gagcgacacc
     3301 agccccattt cccgcaaata catcttgacc gggtctccgg ttttgcccgc gccgggaacc
     3361 agcgcgaggg cttcgggaaa tttggacggc agcgcatcct cgaccggttt tttcaccacc
     3421 agcttttccc cttgctccat gtcgatgatg tcgatgtttt tttctccaaa cagcatgata
     3481 atatcatcga tttgctccga ggaattcaaa tcaggcggca acatgtcatt cacgtcgtca
     3541 taggttaaaa accctttttc ttcccccagg gaaagcagct tcttaaattc gtcggattgt
     3601 atttctttcg ccatttggat gcccccgaat gttatgattg gttcaagtat ttatgttctt
     3661 cctttatcaa atgctgcttt tgaaaagtca gctcccggat gagatccgga tcgccgcatt
     3721 cctgcgcctg ccttaacttg tgctgcacct gccgcttttg ttctttgtac catttttcac
     3781 ggattctccg catgttgtcg acaaaattgc gctccaccct gtcatcatcc gtcggaggcg
     3841 cttccacgct cattttgtat agatgctccc gcagcggttt gtcttcatcc gacggcagca
     3901 ggatgttgac atccacgtac cccagaagtt tataggtttg aataattttt gttcccagat
     3961 cctttaacgc gggctccaga aaacactcca gaatattctc atcgtccacc aggagcgcct
     4021 tctgcggata ctccagcatc agccggatca gattgaactc cagaggattc acggccattc
     4081 cgccggatgg ctttttaaca cccgcgccgg atggcgcgga tgccggtttc cgatggattt
     4141 cccttttcag cagcgcctgg tcaatgcccg atttctccgc aatccgcttg atgaaaagat
     4201 tcttttcgat ttcgttgttg attttcccca cgaattccat cgcggttttc accagatcac
     4261 ggttttcttc aaatgttttg ccgtttccca gaacggaatc aatataataa tcgctgatcg
     4321 ccggcgcaag ggcgatcagt tcctccagct tcgccgggcc gtattttttc acaaaatcat
     4381 ccggatccaa gccgtcggga aggattaaag cccgtgcgct catattggcg cctaaaaaaa
     4441 gctccagcga ccggtccagc gccttgcgtc cggccgcatc cggatcaaac agcgcgacga
     4501 catcctgcgt gtaacgccgc aggagctcca gatgatcgcg ggtcagcgcc gtgcccagcg
     4561 tggccacgac attgcgcacc cctgcgttcc acagggagat gacgtcaaaa tagccttcca
     4621 cgatcaggca aaaaccgttc cggcggatcg tctctttggc cttgttgagt ccgtataaat
     4681 ttttcccctt gctataaacc ggcgattccg gcgagttgag atatttgggc tcgccctctc
     4741 cgataatccg gccgccaaag gcgacgattt cgccgaaagg attttcaata gggaacatca
     4801 gtcggccgcg gaagcggtcg tagaagccac cctctttccc ggcgatgacc aggccggcct
     4861 gttcggccat tttcagggac aaaccgccgc cttccacatc atccgccagg gagcgccagg
     4921 cgtccggcgc ataccccaac ctgaattgcc ggacggtttc ctcggtgacg gcccgcttct
     4981 gcaggtagtc gcgggccgct ttgccggcgg gagaggagag ctgacgggca aagtaccgcg
     5041 ccgcccttgc attcaactga aacatcccgt cgcgcaggga ttccttttcg cgtccgtcgc
     5101 tgccgaaggt ccgcaccggc agcaggacgc ccgttttttc cgccagatat ttaacggcct
     5161 cggggaaact cttgttggcg attttcatca gcagcgtgat gacattgccg ccttccccgc
     5221 agccgaagca gtagaaaatc tgtttttcgc ggttgaccgt aaacgaaggg gtcttttcct
     5281 gatgaaaggg acaaagcccc agataattcc ggcccgcctt tttcagcgtc agatattcgg
     5341 aggcaagctc gacaatgtcc accctgcttt taatttcttc aatcttgctg tcgtcaaagc
     5401 gcacgaagga tcgcctgccc tgttttgatg gtttgagtcc gtgtcgtgga taacgcgtct
     5461 tattgcgaca gaagcgcttt caccttttca ccggcggctt tcccgtccgc cttgccggta
     5521 atctgcggca tcagcgcctt catcactttg cccatatcct tgacggatag ggcgcccaca
     5581 tcggcaatcg cctttttaat gagcgcttcc acttcttcat ccgacatttg cgcgggcatg
     5641 aaatcctgca ggattttcag ttccgcttct tccttttcca ccagatcctg cctgcccccc
     5701 ttggcaaact gctcgatgga atctttccgc tgcttgacca atccggacag aatctgcatt
     5761 gtttccgtct cgttgagcgg ccgcatcagg tcgatttcct tgttgtggag cgctgttttc
     5821 agcagacgca gcgcggagag acggattttg tcttttgcct tcgccgccgt caccatgtct
     5881 tcgtttaatt tggccaaaat atccattttc ctttacctca cattatccta aaggatcttc
     5941 cagttttctg cctcccagca gatgcatgtg cagatgaaag accacctgcc ctccgtcggc
     6001 gttgcagttg atcgccagac ggaatccgct gtcagccacc tgtttcattt tagcaacctg
     6061 ttgcgcggcg gccatccatc ttcccatgat atccgcattc gtatcaacat ccagcagggt
     6121 cgctatatgt tttttgggga tgatgatgac atgcaccggc gccatggggc tgatatcgtc
     6181 aaaagccagg acagcgtcgt cttcgtaaac ttttttggaa ggaatgtctc ccttgatgat
     6241 tttacaaaat atgcactctg ccatctgctt ttcctccttc cgattttatg aagccgcttg
     6301 cgttttcgca atcgcgtctt ccagggcgat ggcgcccgtg tagagcgcgc ggccgacaat
     6361 cacgccgtaa aacgagcagt ctttgacggc gagcagggcg tcgatatcgg caagcgtcgc
     6421 cacaccgccc gacgcgatga ccggaatgtt gacggctttc gccagagccc gggtttgttc
     6481 cacattgacg ccggcctcca tgccgtcgcg tgaaatatcc gtataaacaa tggcctggat
     6541 accgtcatga acatagcgct cggccagttc gaccgcattt tggccggtct tctgtgtcca
     6601 gccgcggacc gccacctctc ccgcaagcgc gtcgatcccc aggatgattt ttccccgatg
     6661 ggttcgggca gccgtccgga caaacgcttc atccgcaatg gccgacgtcc ccagaatcac
     6721 actcgacacg ccgttttcca aatagtagtt gacggtttcc atgctgcgga tgccgccgcc
     6781 gacttcaacc ggcactggaa cggatttcgc aatttcgaca agaatcggcg cgtttttggg
     6841 caatccggcg acggatccgt ccagatccac aaggtgaatc ctttgcgccc ctttttgaac
     6901 ccagcgcctg gccatgtcca ccggattttc agcataaacc gtcacccggt taaaatcgcc
     6961 ctgcgcaagc cgcacgcatt ggccgttttt tacatcaacc gccggaataa tcaacacctt
     7021 gcctgactcc ttcacagaac cggtagatca aatatcgagc ggggcaccgc atgaatccga
     7081 agctttttgc cgtcagcgtt caaagccggg aaaggttttg aaaatgtcgt caactcccag
     7141 ctttgtcaac tggcgcgccg tcagccattt cgcgccgccc ttgaagaagg aagacgcctg
     7201 aaaaacatct tccatcagag atttttgtgt tttgtcaaaa ataccccgcc agaggatgcg
     7261 gccgtcgccg acggcaatca gatgaatttc aaaagcaacc gccgcggggc gttccacgga
     7321 ataatcgtac cccaatcttt cccgatagcg atagacaaac cccgccacca ggacatcggc
     7381 gcccagttcg cttccggtct cccggaaagt ctgcagtaaa ggctgtttgc ggagagccgt
     7441 cgagacccac cgatagacac ccgccacctt ttcccgggga atgatctcca cgttcttgta
     7501 gtccatcagc ttatcagtca ccatttcttc aaccattctt tctgcgcctt tggaaatgcc
     7561 gccgctggaa ttcacgctgc cgcaaatcgg gcattgaacg gtggaggacg gctcttccgg
     7621 cagcaccgcc tgaaaaggga tgaccgcgat ccgcgtcgcc tttttttcgg cggcctttgg
     7681 ctcttcctgc gcatgcccaa cgccctggga gagaaggaag aaccccaaaa gccacagggc
     7741 gcagccgatg ctttttatcg aaaatctgtt gtgcgacaca agacgcctga cctttctttc
     7801 aaagcgttcc cttggtggaa agaacgcctg aaatttccgg atggatattc accgcctgcc
     7861 gcaacgcccg ggcaaacgcc ttgaagacag cctcgatgat atgatgactg ttgctgccgt
     7921 attccagggt aatgtgcagg gtaatgccgc tgtgatccgt gaacgccttg aaaaactcct
     7981 tgaccagcgc tggatcaaaa ccgccgattt tcctgcgttc ataccggact ttgtaaatca
     8041 gatagggacg gccgcagatg tccatgtcca cgcggcacag actctcgtcc atcgggacgg
     8101 acgccgaacc gtagcggtta atgcccgtct ttgtgcccag cgcctgccgc accgcctctc
     8161 ccagacaaat ccccagatct tccaccaggt gatggtcatc gatttccgtg tcgcctttgc
     8221 tcttgacaac cagcttgaaa cgaccgtggc gggcaaacag ctccagcatg tgattcaaaa
     8281 agggaatggt tgtatcaatc ctgctgccgg cggatttatc caaatcaatg tccagcgcga
     8341 tatccgtttc cgttgtttta cggacgatct ttgcctttct ggccatgacg cctcccccag
     8401 atcaaatgac tttgttgagc ggatattcaa taatgccctg cgcgcccgcg tcgagaagcc
     8461 gtggaatgat atcgcgcacc gcacccgtat cgataaccgt ttcaatggca aaccactctt
     8521 ccgaatagag ggtggaaatg gtcggggctt tcagcgacgg caagagcttc atgacacgat
     8581 cgaggttttc tttggccaca ttgagcttga ggccgacttt gcccatcgcc aggagcgatc
     8641 cggtcaagag cgtccggatc tgctcgattt tttttctctt ccagggatcg ttccaggctg
     8701 cgcggttggc gatcagctgt gtgttggttt tcatcagttc atgcacgatc ctgagtttgt
     8761 tggcccgaat ggtggatccc gtttccgtga cttccacgac ggcgtccacc agaccgtcga
     8821 cgactttcgc ctcggtcgcg ccccaggaaa actccacgcc gaccttgatc tttctttcgg
     8881 caaaataccg cctggtaaag ttgaccagtt ccgtcgaaat gtgcctgcct tccatgtcct
     8941 cgatcgaacg gatctccgaa tcgtcccgca ccacaagcac ccagcgcgcc tgacgggtgg
     9001 aaactttgga atagatcaga tcctgcacga cctgcacatc ggagttgttt tccagaatcc
     9061 agtcgatgcc ggtcagcccc agatccagat gaccgtcttc gatgtagcgg gacatttcct
     9121 gcgcccggac cagcgtgcag gaaatttccg gatcgtcaat atccgggaaa tagttgcggc
     9181 tgtcgtaggt gatccgccag cccgcttttt gaaataaatc aaccgtattt ttttccagac
     9241 tcccttttgg gatgcccaat ttcaaaacat tcatttgtac acttccttgg gatcaaaaac
     9301 tttcttgccg acaacctcaa aatcgccgcc tttgcgcctg cggaaaaaac aggtgcgatg
     9361 ccccgtgtgg caggcggccc cgcccagttg ctccacctga agcagcagag tgtcctcgtc
     9421 gcaatcaata aaaatatctt tgacgatctg aacatggccg gagctctcgc ccttcagcca
     9481 cagtttttgc cgggaacgac tccagaacgt ggccttcccg gattgcagcg tcgcttccca
     9541 ggaggcccgg ttcatgtagg ccagcattaa aacgtctttt gtttcagcat cctgcacaat
     9601 ggccggaatc aatccgtccg ttttattgaa gtttggttcg atcataagca ccttcctgcg
     9661 aatgcagtaa tttgtctcct ttatgaaatt attttgttta atggatgagt accattaaat
     9721 caagtaatat tttagcttac gaatcatcta tttccggctt gcctaacctt tttattcatg
     9781 gtaaaaaaac gcgaaaattg atcggacgga acgatttgaa tttgtttgcc cggagggatt
     9841 gaatcatgtc tttgctgttg ggaggcgtcg ttgctgcggt catcggactg atcagtttga
     9901 ttttctggtg gtctgatttc ctggccattc tccggggcgc gctgcccatc ttcctgttcc
     9961 tggggggcgc tctggcggtc tatgtcggtt ttgacgatat tcaggacaaa atccgggaag
    10021 aacggcagcg gcaaaatgaa gagctggaga aagccaggga agaagttgaa tcggccaaag
    10081 cccaggccga acgctacaag gatgaactgg accggttaaa ggatgaaaaa aagcaaagct
    10141 gaattcagct tcgcacagca caaatacagc ttgacacagc cggttaaatc aaataatact
    10201 tcaccgtgtt acgagagctt tgaaaaatgt catttcgagg agcgtgagcg acgagaaatc
    10261 ttcatgatgg ctgtttgtta aagatttctc ccttcggtcg aattgtttcg gaaaccggta
    10321 tgttttcatt ttacacaata aaatcctcgc cttataatat ctacatgtat tgatgcctgc
    10381 ttgcccgggt tcttcatcaa ggtaagcagg cttttccgaa agcatcagcg caagttcaaa
    10441 atcagcatca acaatagccc ttggaacgtg tttctatgta tggaaaaaat aataaattgg
    10501 gaggaaacag gtcaaatgga aaagaaaggg ttttttgtaa cgatttcatt actctgtgcc
    10561 atattttttg ttctggtgtt cagttccggc gtgctggcgc agacaacgct gacgtatagc
    10621 atctttttcc cggccaccca cgcacaggcg aaagccggcg atgcttgggc gaaggaaatc
    10681 gaaaaacgca ccgggggcaa agtcaaaatc aacatcttcg ccggcggatc actgacaccg
    10741 gccgatcagt gttttgacgg cgtcgtcaag ggaatttccg atatcggcat gtcctgtttt
    10801 gcctatacac gcggccggct gccgcttctg gaagttctgg atctgccgct cgggtatccg
    10861 aacggcatgg tggcgacgct ggtggccaac gacttagtca aaaagatgaa gcccaaggag
    10921 ctggatgacg taaaggtact gtatctgcat gcccacggcc cgggcctcct gcacacccag
    10981 aagccggttt acaagatgga gcatctcaaa ggaatgaaag tccggtcgac gggtctcagt
    11041 gctaagatcg tggagaatct gggcggcatc tccgtcgcca tgccgcaggg cggaacgtat
    11101 gaagcgctgc aaaagggcgt tgtccagggc acactggccc ccatggaaac cttaaaaggc
    11161 tggaagcagg cccaggtcgt gaaatacacg accgactgca ccaacatagg ctacaccacc
    11221 gcgatgttcg ttgtcatgaa caaaaagaag tgggaatcac tgcccaagga tgtacagaaa
    11281 gtgtttgacg a
//
LOCUS       DAOSRM010000074        11153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5746_length_11153_cov_96.973148, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000074 DAOSRM010000000
VERSION     DAOSRM010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5746_length_11153_cov_96.973148"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLA58_07685"
     CDS             <1..277
                     /locus_tag="PLA58_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62711.1"
                     /translation="TRIFRPTLTAVSAQEIKKGAGVKNFLRAVVIKEKNRYLARLTGE
                     QGSGILKSMVEANAFIVLQEDVSHVKKGENVLVQLLEETLTGADVPA"
     gene            334..1050
                     /locus_tag="PLA58_07690"
     CDS             334..1050
                     /locus_tag="PLA58_07690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOR62712.1"
                     /translation="MRKLMIVVCVALIAVWGCSPEAPKTEDQKAFYALGVHLSKQLSV
                     FDLTTEELKYVQLGMSDAAGGKKLAAEPEANMQKLGELAQARMQKATEKQKELAKPFL
                     EKAAKEAGVKKTASGLIYKEIKAGTGAQARATDIVKVHYVGTLIDGKEFDSSVKRGQP
                     AELPLSQVFPCWTEGVSMMKVGGKSKLICPSEIAYGDRGRPPVIPGGATLVFEVELLD
                     VKDAKTAALQSSPSVKPKAK"
     gene            1174..2703
                     /locus_tag="PLA58_07695"
     CDS             1174..2703
                     /locus_tag="PLA58_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOR62713.1"
                     /translation="MKKQEWFRLLRRLPALAVIFLAAFLMLLPCPSWAEPATPAQEDD
                     NQPRLRSLALANEIWLGDFDQLLEKRVIRVLVPQSRTLYLIVRGKEQGLIGDMVREFE
                     RTLNKKYARKLKKRPLTVVIIPRTRDLLLGDVARGLGDIAAGDITVTEERLKTVDFAA
                     PPDLPGVSEILVAGPSSPAVGAIDDLAGKTVHVRKASSYYESLTLLNNRLIREHRKPV
                     NIILVPDALEDEDLMEMLQAKIFDFTVVDDWMARLWAEVLPGITVRDDIVLRSKGRIG
                     WAIRKNSPRLESEILDFYRIVLKKRGVIEGRIQQYHRNIEKIKDPTGTGEWKRFEQTL
                     SLFRKYGSRYGFDPLMLAAQGYQESRLDQSKRSPAGAIGIMQIMPKTGRSLKVGDIKI
                     TEPNIHAGAKYLDLLMTNYFSDACFSEQDRSLFAFASYNAGPGRISRMRTLAKERGLD
                     ANRWFNNVELVTAEKVGFETTAYVRNIYKYYVAYKLTINSMEEKQKILQQAVADPSRK
                     P"
     gene            complement(2714..4078)
                     /locus_tag="PLA58_07700"
     CDS             complement(2714..4078)
                     /locus_tag="PLA58_07700"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HOR62714.1"
                     /translation="MNVIATSVFDIFKIGPGPSSSHTLGPMKAAGRFRQACAGLKKAA
                     LEKATAVEVSLYGSLATTGKGHGTDRSVIAGLLGWDPETVDPDAFDPLGTDPKAVYTF
                     CAGKKEIPFRAGDIHFDTTPGNLPHPNTMVFRLKAGEGILLEQEYYSVGGGFILCRGE
                     AEHPRPIPPIPYASMHQLRAQMDRLNIGLVELLRRNEAVVSGLDAERTDKGLDAIIDA
                     MVSAVERGLATDAILPGPIGLARKAPVLLRRAQTIPDSPERFVILLNTYALAASEENA
                     AGHRVVTAPTSGSSGIIPGIIYFAAKHLGISRELIREGLLVAGAVGGITRHNASISGA
                     EVGCQGEVGVASAMAAAMIAHIRGCGMAVIENAAEIALEHHLGLTCDPIGGYVQIPCI
                     ERNAVGAVHAYNAYLLASAGDPARQKIDFDAVVEAMLETGRDMSERYKETARAGLAVC
                     SIRC"
     gene            complement(4075..6177)
                     /locus_tag="PLA58_07705"
     CDS             complement(4075..6177)
                     /locus_tag="PLA58_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOR62715.1"
                     /translation="MTNDETNRPAELKKDIGLVSALAIVVGMVLGAGAFMKPPAVMAA
                     AGDSTWALAAWVIGAVFSMAGGLTLCELGVLYPRTGGVYVYLEEIYGPKVAYLYGWML
                     TFIFGPATIGALAGYFSSVFCLLFGIPDHYLPVIGLAVMAFVLFVNSVGVKQAGYLQV
                     LATFCKLIPIVLLAVFGLWKGNGHVLNLSTGVAASATFSVAVIATLFAYDGWAQVASV
                     AGEMKNPGKILPRAVVGGLIFLSVVYIVINVALLKVLSPSEMVALGHDASAIAAQKLF
                     GLYGGNLISVGIMVSIVGGLNGYVMTLSRIVFTMAERNQLPGSGALSRIEPDSKTPVN
                     AGILLVVLSFIYLRMLDADRLTDMAMFAIWIFYMMTFAAVFIARKRFPDAVRSYKVPF
                     YPVTPLVAIGGALYIMYGMILSQPVNAMVSLVLTLAGIPVLYGMTHGLPRFEGFAVSK
                     KYLVLGGAVLILAALLITPKFIDTRPVLRVAVEISNPPIAFEDETGKPAGLDIDIIQS
                     VAGEMGYRIAFRATPLDHIFPALEKDLVDAAISELTVTPERSRQAAFTRSYHQGTLAM
                     LVHADSPATTLADLRGKAVGVKSRSTGEKYMNGRSEYRLWRMSVSSDLLEHFKNGALD
                     AVVFDKPIIDEWLAQGLVRGRSLPLGEQEEYAIAYRKEDTQLGRKIDHALDKLIKSGR
                     LEEIQKKWLISRSERPSS"
     gene            complement(6264..6854)
                     /locus_tag="PLA58_07710"
     CDS             complement(6264..6854)
                     /locus_tag="PLA58_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HOR62716.1"
                     /translation="MTGKLPLVFCALAALLTGCASVQPTRFYTMSPATAPAATAPAVY
                     SLSVGPVWVPAAVDRPQIVLQTGPNQVFIAEFDRWAEPLKEAIPRVVVENLSLMLGAR
                     DIAIFPQSGSADASYRITIDILRFESLPGKAAIVDARWTVRSTKDEKTGSGRAKITET
                     VRAEGYPELVAAHSRALEQVSGQIARTVRDMAGSKP"
     gene            complement(6851..8524)
                     /locus_tag="PLA58_07715"
     CDS             complement(6851..8524)
                     /locus_tag="PLA58_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOR62717.1"
                     /translation="MTDPADLNNLPRSTTVPKRRMRLSVVWIIPILAALVGVGIAVQK
                     ILHEGPTISIVFKTAEGIEAGKTFVRYKDVKIGLVQSVKLSKDFTKVVVTAKIDKSAE
                     GLIVEDAAFWIELPRVTMSGVSGISTLLSGNHIGLGAGKSRRPRHEFVALDMPPAVSI
                     DKPGRQFVLRADTLGSIGIGSPLYYRQLNVGQVIGYDLSKDGRSVEIRVFVNAPYDRQ
                     ITTQTRFWQASGIDVSLGSNGLSVQMQSLLSILIGGIAFEQPLTADADAQPVKEGAAF
                     TLYSNRTAAESRHEEIITPYVLYFKESVRGLSVGAPVTLAGLPIGEVTDVSFEYDSAK
                     EDVRSRVDIVMYPSRFTEYMKTSSGSEHAPLDEQGRRAFIQRMMNRGFRAQLRIGNLV
                     MGQLYIAIDRFPNAPKAKKIDWRKPPYELPVMSSSLEDLETRVSDIIARIDKIPLDAI
                     GADVRKTLETLHQTLKDADRVMNHIDRETVPEINATLQDLRKATATADRALAGADQTL
                     VGKDSPTQQELRAALQEVSRAARAITELVDYLERNPNALIRGKAQEVPK"
     gene            complement(8517..9827)
                     /locus_tag="PLA58_07720"
     CDS             complement(8517..9827)
                     /locus_tag="PLA58_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HOR62718.1"
                     /translation="MTLFPEHIAACPDCDLMQRIPALSSGETARCLRCGRSMAAGKTD
                     PLNRTFAFAVAAAIVLIIANVTPLMGLSAVGRHASTTILGGVLKMWQEGQGITAALVA
                     FCTVVAPTIYITFMLTILIALRKDPAPSWIGRLLRISDVSRTWSMVEVMMLGILVALV
                     KIADLATVIPGIGMFAVFALILLVAAMTVSFDPREVWLRIRWADEAAPPVGEERSSRP
                     GDDRAMPAEERSAARLGFVLCGACGLLSKYADPAEPGYCPRCGKALEVRRRQTIQRTW
                     ALLIAAAICYIPANLMPVMNTTMPAYAEKDTIMNGVILLYTSGSWPLALIVLIASVII
                     PLAKIVTLAYLLVTIQFRQPESQRERIRIYRFVEIIGRWSMLDVFVVTFVVALVQLQP
                     LMSVEPGAGVIFFAAVVVLTMFAAEQFDPRLIWDSPGEKENDHD"
     gene            complement(10051..10641)
                     /locus_tag="PLA58_07725"
     CDS             complement(10051..10641)
                     /locus_tag="PLA58_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62719.1"
                     /translation="MTGRRSLIFLKTGMMVMILICAMTTAVDGMGTKPQQSVPYRKMR
                     MADYQNFIRNWDEKKDPLLYALVSTPAEYRTLFHPAPTMPSRRPFAPDESLYEKEQIL
                     VAARVMPHPKNVEEVFEIEKIIEKRGSLSLYYKFNRPEPAAEWQGKIYLAVRIPRRFY
                     QKIFFFENGKPVGELNVAAGQWSVPALDPSTGKTGD"
     gene            10920..>11153
                     /locus_tag="PLA58_07730"
     CDS             10920..>11153
                     /locus_tag="PLA58_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemopexin repeat-containing protein"
                     /protein_id="HOR62720.1"
                     /translation="MKKNTLIVIAGLFFLMAWTTASAERYVPDQHPTGIVNWGNGKAY
                     FFSGGQYIRYDIASDKADPGYPKNINAETWPGVI"
BASE COUNT         2431 a         3279 c         3274 g         2169 t
ORIGIN      
        1 tacccggatt tttcgcccga cactgaccgc cgtcagcgcc caggaaatca aaaagggcgc
       61 gggcgtcaaa aattttctgc gcgcggtggt tataaaagaa aaaaatcgtt acctggcgcg
      121 cctgaccggc gaacaggggt ccggcatttt gaagtccatg gtggaagcca atgctttcat
      181 tgttttgcag gaagatgtct cccatgtgaa aaagggagag aacgtattgg ttcaactgct
      241 tgaagaaaca ctgaccgggg cggatgttcc cgcctgaatt tggcgcccga tcccagggca
      301 ggaataaaga aagggtaaaa gaaaggatgc tttatgcgta aattaatgat tgtggtttgt
      361 gtcgccctga tcgccgtctg gggatgttcg cccgaagcgc ctaaaacgga ggatcagaag
      421 gctttctacg cgctgggggt tcatctcagc aagcaactgt ccgtttttga tctgacgacc
      481 gaagagttga aatatgttca actgggaatg tccgacgcag ccggcggcaa aaagctggcg
      541 gcggaaccgg aagccaacat gcagaaactg ggtgaactgg cccaggcccg aatgcagaaa
      601 gccacggaaa agcaaaaaga gctggccaaa ccgtttctgg aaaaagccgc gaaggaagcc
      661 ggcgtgaaga aaaccgcctc cggattgatc tataaagaaa tcaaagcggg cacgggcgct
      721 caggccaggg ccacggacat cgtcaaggtc cactacgtgg gcacgctgat tgacggcaag
      781 gaatttgaca gctccgtcaa gcgcggccag cccgcggaac tgccgttgag ccaggtgttc
      841 ccctgctgga cggagggggt cagcatgatg aaggtgggcg gcaaatccaa actgatctgc
      901 ccttcggaga tcgcctacgg agaccggggc cgtccccccg tgattcccgg cggcgcaacg
      961 ctggtctttg aagtggagct tctggacgtg aaagacgcca aaacagccgc attgcagtct
     1021 tcgccctccg taaaaccgaa ggcaaaataa ccccggcccg caagcgcggc cgttgattca
     1081 gaagacccgg aagggggtgg caaagggcct ccccctttct ttaagtcttg cagtctgttt
     1141 cctcttcgat tgccgggatg aaaggggatg ctgatgaaaa aacaagaatg gtttcgtctt
     1201 ctgcgccgtc ttccggcact cgccgtgatt tttcttgccg ctttcctgat gctcctcccc
     1261 tgcccctctt gggccgaacc tgcaacaccg gcgcaggagg acgacaacca accccgcctg
     1321 cgcagcctgg ccctcgccaa tgagatctgg ctgggcgatt tcgatcagct cctggagaaa
     1381 cgcgtcatcc gcgtcctggt tccgcagagc cgcaccctgt atttgattgt caggggaaag
     1441 gaacaggggc tgatcggcga catggtcagg gaatttgagc gcaccctcaa taaaaaatac
     1501 gccagaaagc taaaaaaacg cccgttgacc gtcgtgatta ttccacggac gcgggacctc
     1561 ctccttggcg atgtggcaag agggctcggc gatattgccg ccggcgacat cacggtgacg
     1621 gaggagcgcc tgaagacggt ggactttgcc gcgccgccgg acctgcccgg cgtgtcggaa
     1681 atcctggtcg cgggtccgtc gtctcccgcc gtgggcgcca ttgatgatct ggccggaaag
     1741 accgtgcatg tgcgcaaagc ctccagctat tatgaaagcc tgaccctttt gaacaatcgg
     1801 ttgatccggg aacacaggaa acccgtcaac atcatcctcg tcccggatgc gctggaagac
     1861 gaagacctga tggaaatgct tcaggcgaaa attttcgatt ttaccgtcgt ggacgactgg
     1921 atggcccgcc tgtgggcgga agttctgccc ggcatcaccg tccgggacga catcgtgctg
     1981 cgcagcaaag gcaggatcgg ctgggccatc cgcaagaaca gcccgcggct ggaatcggaa
     2041 attctggatt tctaccgtat cgtcctgaaa aaacgcggcg tgatcgaagg ccgcattcag
     2101 cagtaccacc ggaacatcga aaagatcaaa gatccgaccg gcacggggga atggaagcgg
     2161 tttgaacaaa ccttgagcct gtttcggaaa tacggcagcc ggtacggatt tgatccgctg
     2221 atgctggccg cccagggtta tcaggaatcc cggctggatc agagcaaacg cagccccgcg
     2281 ggcgcgatcg gcatcatgca gatcatgccc aaaacgggcc ggtcgctgaa ggtgggagac
     2341 ataaagatca ccgaacccaa tattcacgcc ggcgccaagt atctggacct gctcatgacc
     2401 aactactttt cggatgcctg cttcagcgag caggaccgct cgctttttgc ctttgccagc
     2461 tacaacgctg gaccgggaag gatctcgaga atgcggacgc ttgcgaaaga gcgcggcctg
     2521 gacgcgaacc ggtggttcaa caacgtcgag ctggtcaccg ccgaaaaagt gggatttgag
     2581 accaccgcct acgtccggaa catttacaaa tattacgtgg cctacaagct gacaatcaac
     2641 agcatggagg aaaaacaaaa aatcctccag caggccgttg cggatccatc ccgcaaacca
     2701 tgacggaccc gcctcaacac cggatggaac aaacggccag tccggcccgg gccgtttcct
     2761 tgtacctctc cgacatatcc cgacccgttt ccagcatcgc ttccaccacg gcgtcgaagt
     2821 cgatcttctg gcgggccgga tcccccgcgg acgccagaag ataggcattg taggcgtgga
     2881 ccgctccgac ggcgttgcgt tcaatacagg gaatttgaac ataaccgccg atgggatcgc
     2941 aggtgagtcc cagatggtgc tccagggcaa tttcagcggc gttttcaatg acggccatcc
     3001 cgcagccccg aatgtgcgcg atcatggctg ccgccatggc tgaggcgacg ccgacttctc
     3061 cctggcagcc gacttcggct ccggagatgc tggcgttgtg gcgggtaatg ccgcccacag
     3121 ccccggcgac gagaagcccc tcccgaatca gctcccggga aattcccaga tgctttgccg
     3181 caaaataaat aattccggga ataattccgg atgagccgga cgtcggcgcc gtcacgaccc
     3241 ggtgacccgc ggcgttttct tccgaggcgg ccagcgcgta agtgttgagc aaaatgacga
     3301 atcgctccgg cgaatcgggt atcgtttgcg cccgacgcaa aagaaccggc gccttgcggg
     3361 ccagaccgat gggtcccgga agaatggcat ccgtagccag gccgcgttca acggcgctga
     3421 ccattgcgtc aatgatcgca tccagtccct tatccgttcg ctccgcgtcc agaccggaaa
     3481 ccaccgcctc attgcgccgc aggagttcga cgagaccgat gttcagccgg tccatctgag
     3541 cgcgaagctg gtgcatgctg gcgtagggaa tcggcggaat cggccgcgga tgttccgcct
     3601 ctcccctgca gagaatgaag ccccctccga cggagtagta ttcctgctcc aggagaatgc
     3661 cctctcccgc tttgaggcga aaaaccatgg tattggggtg cgggagattt ccgggggttg
     3721 tgtcgaaatg aatatcgccc gcgcggaaag ggatctcctt tttgccggcg caaaaggtgt
     3781 aaacggcttt tggatccgtg ccgagcggat caaaggcatc cggatcgacg gtttccgggt
     3841 cccatcccag caggccggca atcacggagc gatccgtccc gtgtcctttc ccggtggtgg
     3901 cgagcgaccc gtacagagag acctcgacag ccgtcgcctt ttccagagcg gcttttttca
     3961 gtccggcgca ggcctgccgg aagcggccgg ccgccttcat ggggcccagc gtgtgagaac
     4021 tggaaggccc cgggccgatt ttgaagatat cgaataccga tgtcgcaatc acgttcatga
     4081 tgaaggcctt tccgagcggc tgatcagcca cttcttctga atttcttcca gtcggccgct
     4141 tttgatgagt ttatccaacg catgatcgat tttccggccg agctgcgtat cttccttccg
     4201 gtaggcgatg gcatattctt cctgctcgcc gagcgggaga gaccggcccc tgacgagccc
     4261 ctgggccaac cactcgtcaa tgatcggctt gtcgaagacg acggcgtcca gggcgccgtt
     4321 cttgaagtgc tccaacaggt cggaagaaac gctcatccgc cacagacgat attcggaacg
     4381 cccgttcata tacttctcgc cggtcgaacg gctcttgacg ccgaccgcct ttccgcgcag
     4441 atccgcgagc gtagtcgccg gactgtccgc gtgcaccagc atcgccaggg ttccttgatg
     4501 atagcttcgg gtgaaggccg cctgccggct tcgttccggg gtcactgtca gttcgctgat
     4561 ggcagcatcc accagatcct tttccagggc cgggaaaata tggtccagcg gcgtggcacg
     4621 gaaggcgatc cgatacccca tctccccggc gaccgactga attatatcga tatccagccc
     4681 cgcgggcttg cccgtttcgt cctcaaaggc aatgggcgga ttggagattt caacggcgac
     4741 ccgcagcacc ggccgggtgt cgataaactt cggtgtaatc agaagggcgg cgaggatgag
     4801 aacggctccc cccaggacga gatatttttt gctgaccgca aaaccttcga agcggggcag
     4861 cccatgggtc atgccataga ggaccgggat tcccgccagc gtcaggacca gagagaccat
     4921 ggcgttcacg ggctggctca gaatcatgcc gtacatgatg tagagggcgc cgccgatggc
     4981 gacaagcggc gtcacgggat aaaagggaac cttgtagctg cggaccgcgt cgggaaaccg
     5041 cttccgggcg ataaacacgg cggcgaaggt catcatgtag aaaatccaga tcgcaaacat
     5101 ggccatgtcg gtcaggcggt cggcgtcgag catccggaga tagatgaaag acaggaccac
     5161 caggagaatg ccggcgttca cgggcgtctt gctgtccggt tcgatccggc tgagcgcccc
     5221 cgagccggga agctgattgc gttcggccat cgtgaacacg atgcgcgaga gggtcatcac
     5281 gtagccgttc agaccgccga caatcgacac catgatgccc acggaaatga ggttgccgcc
     5341 gtagagcccg aaaagcttct gagcggcaat cgcggaggcg tcatggccca gggcgaccat
     5401 ctcggaaggc gataaaacct tgagaagggc gacgttgatg acgatgtaca caacggacag
     5461 aaagatcagc ccgccgacga cggcccgcgg caggattttg ccgggattct tcatttcccc
     5521 cgccacggaa gccacctgcg cccagccgtc gtaggcaaag agggtggcaa tgacggcgac
     5581 ggagaaggtc gcggatgcgg cgaccccggt cgagaggttc agcacatggc cgtttccttt
     5641 ccagaggccg aaaacggcaa gcagaacgat ggggatcagc ttgcagaatg tcgcgagcac
     5701 ctgcaggtag cccgcctgct tgacgccgac gctgttgaca aacagaacaa acgccatcac
     5761 ggccaggccg atgacgggaa ggtagtggtc cgggattccg aacaggaggc agaaaacgga
     5821 gctgaaatag ccggccagcg cgccgatcgt cgccggcccg aagatgaagg tcagcatcca
     5881 gccgtagaga taggcgactt tggggccgta gatttcttcc aggtaaacat agacgccgcc
     5941 cgtgcgggga tacaggaccc ccagctcgca aagcgtgagc cctccggcca tgctgaacac
     6001 cgctccgatc acccaggcgg ccagcgccca ggtggaatcg cccgccgcgg ccatgaccgc
     6061 tggcggtttc atgaaagcgc cggcgcccag caccatcccg accacgatgg cgagagcgct
     6121 caccagtcca atatcttttt tcagttcggc cggcctgttc gtttcatcat tcgtcatggg
     6181 attctccccg ggggtcctcc atcggcgcgg atagccctgt atgatagtcc ctcgctttca
     6241 tcccggattc gcaggcagcg actttacggt ttgcttcccg ccatatcgcg aaccgtccgg
     6301 gcgatttggc cgctcacctg ctccagcgcg cggctgtgcg ccgcgaccag ttcagggtaa
     6361 ccttccgcgc gaaccgtctc ggtaatcttc gcgcggccgc tcccggtctt ttcatccttc
     6421 gtggaacgga cggtccaacg ggcatcgaca atggccgctt tgcccggcag ggattcaaag
     6481 cgaagaatgt cgatcgtgat ccggtaggat gcatccgccg accccgactg gggaaaaatc
     6541 gcaatgtccc gcgcgccgag catcagggaa agattttcga cgaccacgcg gggaatcgcc
     6601 tctttcagcg gttcggccca acggtcgaac tcggcgataa aaacctgatt cggaccggtc
     6661 tgcaaaacga tctgcggccg gtcaaccgca gccggcaccc agacgggacc caccgacagg
     6721 gaatagacgg ccggcgccgt cgcggccggc gccgtcgcgg ggctcatggt gtagaaccgc
     6781 gtcggctgaa cggaagcgca gccggttaaa agcgcggcca gcgcgcaaaa gacgaggggc
     6841 aattttcctg tcatttgggc acctcctgtg ctttgccgcg gatgagggca ttgggattgc
     6901 gctcaaggta gtccaccagt tcggtgatcg cgcgcgccgc gcgtgacact tcctgcaggg
     6961 ccgcccgcaa ctcctgctgc gtcggcgaat cttttccgac cagtgtttga tccgcgccgg
     7021 ccagggcacg atcggccgtc gcagtggcct tgcgcaggtc ttgcagggtc gcattgatct
     7081 ccggcacggt ttcccggtcg atatggttca tcacccggtc tgcgtctttc agcgtctggt
     7141 gaagggtttc cagcgttttc ctgacgtctg caccaatagc atccagcggt attttatcga
     7201 ttctggcaat aatgtcgctt acgcgggttt ccagatcctc cagcgaactg gacatgactg
     7261 gcaattcata cggcggcttg cgccagtcga tcttcttcgc tttgggggcg ttcggaaaac
     7321 ggtcaatggc gatgtaaagc tgtcccatga caagattgcc gatgcgcagc tgcgcgcgga
     7381 agccccggtt catcatccgc tggatgaaag cgcggcggcc ctgttcatcc aggggcgcgt
     7441 gttcggagcc ggaggatgtt ttcatatatt ccgtgaagcg ggacggatac atcacaatgt
     7501 ccacccgcga ccggacatct tcctttgccg aatcgtattc aaagctgaca tcggtgacct
     7561 cgccgatcgg cagaccggcg agggtcacgg gggcgccgac ggaaagacca cgcaccgatt
     7621 ccttgaaata gagaacgtag ggagtgatga tctcttcatg ccggctctcg gcagccgtcc
     7681 ggttgctgta cagcgtgaag gcggcgcctt ccttcaccgg ctgggcatcg gcatccgccg
     7741 tcagcggctg ttcgaaggcg atgccgccga tcaggatcga cagcagagac tgcatttgca
     7801 ccgacagccc gtttgacccc agcgaaacat cgatgccgct ggcctgccag aagcgcgtct
     7861 gggttgtgat ctggcggtca tagggagcgt ttacaaaaac tctgatttca acggagcggc
     7921 cgtctttgga cagatcatag ccgatgacct gtccgacatt caactgacgg taatacaggg
     7981 gggacccgat gccgatggaa cccagcgtat cggcgcgaag cacgaactgg cggccgggct
     8041 tatcgatgga gacggcgggc ggcatgtcca gtgcaacaaa ctcgtgcctc ggccgtctgg
     8101 attttccggc tcccagcccg atgtggttgc ccgacaaaag cgtgctgatg ccgctgacgc
     8161 cgctcatcgt aacgcgcggc aattcgatcc agaaggcggc atcctcgaca atcagtccct
     8221 cggcgctctt gtcgattttg gccgtcacca cgacttttgt gaaatctttt gaaagcttca
     8281 cgctctgcac caggccgatt ttaacgtcct tgtagcggac aaacgtcttt cccgcctcga
     8341 tgccctccgc cgttttgaag acgatgctga tggtcgggcc ctcatgaaga attttctgta
     8401 cggcgatgcc gacgccgacc agcgccgcca gaatgggaat aatccagact acggaaagcc
     8461 gcattcgcct cttcggcacg gtcgtggagc gcggtagatt gttcagatcc gccggatcag
     8521 tcatgatcat tctccttttc accaggggaa tcccatatca aacgggggtc aaactgctcg
     8581 gccgcaaaca tggtcagcac gacgacggcc gcgaaaaaga tgacgccggc gcccggttcc
     8641 acggacatga gcggctggag ctggaccagc gcgacgacga acgtcacgac aaacacgtcc
     8701 agcatcgacc agcggccgat gatttcaacg aaccggtaga tccggatgcg ttcgcgctga
     8761 ctctccggct gacgaaactg gatcgtgacc agcaggtagg ccagcgtgac aatcttggcc
     8821 agcggaatga tgacgctggc gatcagcaca atcagcgcaa gcggccagga gcctgacgtg
     8881 taaagcagaa tcaccccgtt catgatcgtg tctttctcgg cgtacgcggg catcgtcgtg
     8941 ttcatcacgg gcatcaggtt ggcgggaatg tagcagatgg ccgccgcgat cagcagggcc
     9001 cacgtgcgct gaatcgtttg gcggcggcga acctccagcg ccttgccgca gcgcggacag
     9061 taccccggtt cggccggatc ggcatatttt gacagcaaac cgcaggctcc gcacagaacg
     9121 aacccaagcc tggcggcgga ccgctcctcc gcgggcatgg cccggtcatc cccggggcgc
     9181 gatgatctct cctcgccgac cggcggcgct gcctcgtccg cccaccggat tcgcagccag
     9241 acctcgcgcg gatcgaagct gacggtcatc gccgcaacca aaaggatcag cgcaaagacg
     9301 gcaaacatgc cgatgcccgg aatcacggtt gccagatcgg cgatttttac cagcgccacc
     9361 aggatgccga gcatcatcac ttccaccatg gaccacgtcc tgctgacatc tgaaattctg
     9421 agcagtctac cgatccacga aggcgccggg tcttttcgaa gggcgatgag aatcgtcagc
     9481 atgaacgtga tataaatcgt cggagcgacc accgtgcaga aggccacaag cgcggccgtg
     9541 attccctgtc cctcctgcca cattttcaac acgccgccta agatggtcgt gctcgcgtga
     9601 cgcccgacgg cggaaaggcc catgaggggg gtcacgttgg cgatgatcag gacgatggcc
     9661 gcggcaaccg cgaaggcaaa tgtccggttt aagggatcgg ttttgcccgc ggccatggag
     9721 cggccgcagc gcaggcaacg cgccgtttcg ccgctggaga gcgcgggaat ccgctgcatc
     9781 aggtcgcagt cgggacaggc cgcgatatgt tcgggaaaaa gcgtcatgac gacctttctc
     9841 atccggcagt cgaacaattt tgtcggagtg gtttattcaa ccatggaatc cgccaaacgt
     9901 ttggagacaa atcgggaacc ttcttttgcg aaaggcacta taaaaaatgt ggtcctgcat
     9961 gtcaatgaaa tcttgcagtc aaggttgaaa gcctcccggc gcctgaaaca gtctttttgg
    10021 ggaaagcttc catctcgtcg ccggatttta ttaatcccca gtttttcccg tcgacggatc
    10081 cagggccgga accgaccact gccccgccgc aacattcaat tcccccaccg gttttccgtt
    10141 ttcaaagaaa aatatttttt ggtaaaaacg ccggggaatt ctgacggcca gataaatctt
    10201 tccctgccat tcggccgcag gctccggcct gttgaacttg taatacagtg acaggctgcc
    10261 gcgtttttcg ataattttct cgatttcaaa cacctcctcc acattcttcg ggtgcggcat
    10321 gacccgggca gccaccagaa tctgttcctt ttcatacaga gattcatccg gagcgaaggg
    10381 gcgcctcgac ggcatcgtgg gcgcgggatg gaagagggtc cggtactcgg cgggtgttga
    10441 aaccagggca tagagcaggg gatccttttt ctcatcccag ttcctgatga agttctggta
    10501 atccgccatg cgcatttttc tgtaaggcac ggactgctgc ggctttgtgc ccatgccgtc
    10561 cacagcagta gtcatcgcgc atatcagaat catcaccatc attcctgttt tgaggaaaat
    10621 caatgatcgt ctgccggtca tgtccagtcc tcccgatcca ttttctggcg tcgttctgcg
    10681 cggaacctcc tgtctgcgcg cccgaaaagc cgtccaacaa cgggtgcatt atatcatttt
    10741 ccggttttca tgcagcaatt tattgcacgg gcgggaagag gaaacggctg ttttttgtgt
    10801 tttttaaaaa aaacgaatag tgctatgatg cagccgttgc cgcaaaaaag cggcaagcgg
    10861 aaaaccatca ttcaaagcat aatggattgt cacaaatacc gtcttacagg gagtctcgca
    10921 tgaaaaagaa tacgctgatc gtcattgcag gcctgttttt tctgatggca tggacaacgg
    10981 caagcgccga aaggtacgtt cccgatcaac atcctacggg gatcgtgaac tggggaaacg
    11041 gcaaggccta cttcttcagc ggcggccagt atatccgata cgacatcgca tccgacaaag
    11101 ccgatcccgg ctatccgaaa aacatcaatg ccgaaacctg gcccggcgtg atc
//
LOCUS       DAOSRM010000075        10912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5895_length_10912_cov_106.396242, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000075 DAOSRM010000000
VERSION     DAOSRM010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5895_length_10912_cov_106.396242"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLA58_07735"
     CDS             <1..262
                     /locus_tag="PLA58_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62721.1"
                     /translation="RFLPSVEMTKMAVIQSSHAGFPGGNQVFPLPPDSRPRETIEEKP
                     AGVKRKRQRQNMRINITLSEAKHLDKHHSPRAHPVSRGEVLS"
     gene            329..916
                     /locus_tag="PLA58_07740"
     CDS             329..916
                     /locus_tag="PLA58_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62722.1"
                     /translation="MKNKRYWLIVAVLGMTLACPAFGGTAFGYEKIVDFILKVHPDVP
                     ASAIPDDFLKTGSDDGYGYAHEGYIDGFKIICRSKGGVVKSLHAQKQFNTGDLSAEHT
                     YYNILGNVLAKLIKKYNGTLVNEKGRGESTDKVPYDYGEYCYYTALKGAGKTIKTKDY
                     KLGFWYDLGVWKRCDNVSYNGENPGNVIIEFRPGN"
     gene            1026..2117
                     /locus_tag="PLA58_07745"
     CDS             1026..2117
                     /locus_tag="PLA58_07745"
                     /inference="COORDINATES: protein motif:HMM:NF027862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HOR62723.1"
                     /translation="MKKWTFFLVMLFLAVSVFSADAQKKITAKGMAANQRNMDIACSR
                     ALDEAQRNAVEQATGVMITSTTSVEDFQVKMDRILSESKGFINTYKVLSEKKSGTDCE
                     VTIEADVSVGKLRDRMTAVNLIMARKSKPRVMLVFKDSVAEAVMAKYLIQHNFKLVDA
                     DVSSKNREYDRLKNTAEAGDIRGLAQHYGAEVIIVGTVDADSKSSTIYNIEMNKNTVV
                     VTGKVINGDTGEIITTASETKSDNKMKGDYKALTEEVSTKLITKLVDEVLEKWSKELA
                     NTATVKLIVTGLDEDTLEDFKAELGEEIKGVKDVNERFYAGGKAEFDLEVEGNAQDVA
                     RDLRKITVNKRKIKIKSRSQNRVEAVLLP"
     gene            2158..4143
                     /locus_tag="PLA58_07750"
     CDS             2158..4143
                     /locus_tag="PLA58_07750"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOR62724.1"
                     /translation="MKSRKAYMAIACAVVLLMFAACVGTNESLKQGQEFASQNRWDEA
                     MIYFQKAVSEEPDNQEFKNELARAKQEVAKSRLARAKQSYAAAGQNFMALERLSKEAD
                     EILKLDPNNPESKSFSATVNGQVNNLKTSLKNLYQQAEADMQKEDWLAAIDKLKQVNA
                     IFPNYEDTGNRLRRSLSEGTRAMYQQGTKLAKQEDWKGAAESFKAVMDVNPKFLDAAQ
                     QYANAVKNDNPAYFTAEAAKAESAGKLDRAIAMYEKALEYPQADPKLNDSLAALKTKA
                     SQIFLNESIELLKQDRLYGSFKKLDRAGKYSTALRGDPAYGEHANRLCEALMKRANKF
                     SEKELWGNALVWLQLIDKINPDYEGLFQKMNEVKDPISRRVRKSIAVFDFGSPSSEKD
                     AGKIAANRLLAYLHQNASVDLRIIERENLQSILREMQLSQTGLVDIKTAQSVGKMRGI
                     DTFIMGDVLQFSAKYTDNPTTNQVKVLVNEEDVRNPNFDAWLLRNPKPSEEDLKLAPP
                     MTTKKKDYQFLPNKQGVARINAMLEISYKLVDTQSGEIIANNITGKLVKEDKYQEGLA
                     IAGIKADPLELPTEGEVLDQLAKEKIAEMGRNVLKHFQSLEVEYFNKGQDLQKRRNYE
                     AATEKYTDAIFDEKLKTISTPISQKAAELIELINEFN"
     gene            4158..6419
                     /locus_tag="PLA58_07755"
     CDS             4158..6419
                     /locus_tag="PLA58_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62725.1"
                     /translation="MLKKILAGAVLFLLMLLAETGFLHAQEIKLSVFNFGTANIEASG
                     LGTSVTNALISNLSRNPSISILDRKDLETFLNLNDLQQNDQLDNVVNIGSRLGLDFIV
                     VGSVAKRGSAITVKCGLVQIDKRKEVYSTQVRAQGESALSAEIAKLASLITSVLTKSS
                     TGPESTSAPGTGGGPGAGAPVNFKGVPGNKKIILRWQPVPGFAAAGYEVYRALNANGP
                     FAMLGQTDKTEFADQNVENDISYFYRIRAYDRLSRLSDYTPVVSARTDFAPNPPIILK
                     TEGRAKSMLIVWTPNPAKSVDPSPLVGYKIYRAAEEDGSYQEVNKLLISDLADSGDGK
                     IYYRATGLPDGQTFFYRLAAYNAKGIESELSHPVKGTTLPIVSSVNASGQLIREVKLT
                     WAGISSPFLTAYNIYRSTKSDGGFLKIKRISAAENTARYEYSDTEGLGDNTPYYYTVT
                     AEDDLKAETSPSAVVAATTRDIPPRPEKFTARGGLVKKVELTWQAAPQPEVEGYYIFW
                     STEKTGKYELLKKIQGRENNRYVDESRGFNPLEDGTAYYYMLTSYNKVDAQSKQAQAG
                     ATTKPRPKKPTGLKGASTRVKEAPLTWQANPEKDIQVYLIQRAEGDRDNFSSIGEAAA
                     TLYTDANLKDGTTYRYRIQAKDADNLLSDFSDVIVVTTKPRPQPPENLQGKYESGKAV
                     LSWTPNKEADISHYIIYEKKFIGTEKIAESNTASYADGSLSPGKSRNYVVTAVDRSGL
                     ESDVSAELKVIAK"
     gene            6534..6932
                     /locus_tag="PLA58_07760"
     CDS             6534..6932
                     /locus_tag="PLA58_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOR62726.1"
                     /translation="MSLLAYLSPSWRDEALQRLQTELTPEKMNNVTTSMSNIYKNCPG
                     GSEQFLFVECKDGKVTRVETGSGEPPQAEFRIIGDYETFARISRAELGAQKALMTGKL
                     KLKGNLARALRLAAVADRLNKVLSRIPTQY"
     gene            6947..8170
                     /locus_tag="PLA58_07765"
     CDS             6947..8170
                     /locus_tag="PLA58_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOR62727.1"
                     /translation="MTRILDPQDPYYIDNPGRVKAIQAEMARRGIDVYLGSRIRTMSF
                     VMDTFCPWRSYVIIPSDGQPVFFTFVVDALRIADETWIDPDRVRAYGPMGGMDQVSAI
                     TDFIKEELGIRKGRLGFEDGISTYTAEGNLSHWEYTHFKDALPDFEWINSHDIIDALS
                     LIKDKGTIERFRRASLIVDEGHKAAREALSNGGYKGMTETVIAGIAALAMRRAGSVSE
                     WNFAGLNEIASGVRTGLGACTPPTTKEIQAGEPLMLDFHAMFKLALGDHSHNYLIGPA
                     TRRQRWHADNFVAIIQKVIDNYRAGTTPGTLAAIMMDFAESRGCGDFIQPACEHGIGL
                     FGDEWRIGAVVNPDLPYWTDQDHVYQENEMVVCAMQYASPDEHIGFRYENDIVITRDG
                     CEVLSKYPLGIEEIS"
     gene            8192..9106
                     /locus_tag="PLA58_07770"
     CDS             8192..9106
                     /locus_tag="PLA58_07770"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR62728.1"
                     /translation="MKASRVPWPIDLAGFFWQRKILGRSIPLLASFKVTHRCNLTCRA
                     CPFHLRPHADISHMSRDTAVRALETLSSLGTRIVVFEGGEPLLWRDGKHRLHDLVDCA
                     RRHFLRVAVTTNGTLPLDAPADTLWVSLDGWKETHDDLRSNSFDRVLAHLEKTKHPRV
                     FVHCTINRRNWTDLERMARQIRKLPTVRGMTFQFFYPYGLGEDDLVLAPQERRAAVGT
                     LLGLKKKGYPVCQSSTRLRSMINNRWRCHDDLLVNVDPDGSIVQGCYVKNRGTVNCDA
                     CGFTPVAEASGALDLIPGSLWAGWKLFL"
     gene            9333..>10912
                     /locus_tag="PLA58_07775"
     CDS             9333..>10912
                     /locus_tag="PLA58_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOR62729.1"
                     /translation="MQKRNAGHVVAAVLFMILMVPGVSAAQSASRQNGAVSTRETCYQ
                     CHFQIKSLKEGSKHAALSCDICHSGAKEHLQSFRNKPATAIDQAACGKCHGNQLNSFM
                     RINYSAQARKEKGVPTGRSPLQEKLLAPHGFTKEHNEPRAHVFMVTDQFAVDRFIGGR
                     FQYKKGLAGIHQTGKAWDVLYDTGRELPETAKAGNATCIKCKTTDHILQWKYMGDKDP
                     KARWDRSSDIVAFSKATQNPMGCIHCHDAHGAAPRVVRDALIDAIDRDGAQTFARNGK
                     TDLKVVDFRGFRKIGVMSKTDSRMMCAQCHVEYACNTGFEFDSGKKVDYDDRRTNHYP
                     LKNANDILAHYKKLNYYDFKHAVTGARLVKLQHPEAETYWGSAHDRAGVQCHQCHMPK
                     AKDQGGKMYTNHGVIRPIQSIKDACLGCHPNSSEEEKHYQIETAQNYTRGKMRKAEYW
                     LAKLIDAYEAAQKAGVPEATLAQAREKHEEAHVLWEWWTAENSDGWHNPQLARESLTA
                     SIIASKKGVDILTRARAT"
BASE COUNT         2871 a         3110 c         2993 g         1938 t
ORIGIN      
        1 aagatttctc ccttcggtcg aaatgacaaa aatggccgtt attcaaagct ctcatgccgg
       61 gttccccggg ggaaaccagg tttttcccct gccaccggat tcccgaccgc gagagacaat
      121 agaagaaaaa ccggccggcg tcaagcggaa aaggcaaagg caaaacatgc ggataaacat
      181 cactctctct gaagcaaaac accttgacaa acaccattcg ccaagggcac atcccgtcag
      241 ccgcggcgaa gttttatctt gacttatgaa agcgccggtt ttacaaattc agacagaatc
      301 ctgaatatga agaaatgagg gaaaatccat gaaaaacaaa agatattggc tgattgtggc
      361 ggttctggga atgacgctcg cttgccctgc attcggcgga acggccttcg gctatgaaaa
      421 aattgttgat ttcattctca aagtgcaccc ggacgtaccg gcttccgcca ttcccgacga
      481 ttttctgaaa acgggcagcg acgacggcta cggctatgcg catgaagggt atatcgacgg
      541 tttcaagatc atctgccgct ccaaaggcgg tgtcgtcaaa tccctgcacg cgcaaaagca
      601 gttcaacacg ggtgacctga gcgccgaaca cacctactac aacatcctgg gaaatgttct
      661 ggccaaactg atcaagaaat acaacggcac attggtcaac gaaaaggggc ggggggaaag
      721 caccgacaag gtgccgtacg actacggaga atattgctac tatacggcgc tgaaaggagc
      781 gggaaagacg atcaaaacca aagactacaa actggggttc tggtacgatc tcggcgtctg
      841 gaagcggtgc gacaacgtca gctacaatgg ggagaacccg ggaaacgtca tcattgaatt
      901 ccggccgggc aactgacgct ctgaccggaa acttccgacc tgctttcaac ttgacaccca
      961 tggtcaaatt cagtatgtat aacgccaatg attgaaaaaa attacaaaat caaggaaaaa
     1021 ctttcatgaa aaaatggacc ttctttcttg taatgctgtt tttggctgtt tcggtttttt
     1081 ccgcagacgc ccagaaaaaa atcaccgcca aagggatggc cgccaaccag agaaacatgg
     1141 acatcgcctg cagcagagcc ctggacgaag cgcagcgcaa tgccgtggaa caggcgacgg
     1201 gcgtgatgat taccagcacc accagcgtgg aggattttca ggtgaagatg gaccgcattc
     1261 tttcggaatc caagggcttc atcaacacct acaaagtgct gtccgaaaaa aaatccggaa
     1321 cggactgcga ggtaaccatc gaggcggatg tcagcgtggg aaagctgcgc gacaggatga
     1381 cggccgtcaa tctgatcatg gcgcgcaaat caaagccgcg ggtcatgctg gtgttcaagg
     1441 attccgtagc cgaggccgtc atggccaaat acctgatcca gcacaacttc aaactggttg
     1501 acgcggatgt gtccagcaaa aacagggagt acgatcgtct gaagaacacg gcggaagccg
     1561 gagacatccg gggactggcg cagcactacg gggcggaggt cattatcgtc ggaacggttg
     1621 acgcggacag caagtcctcc acgatttaca acatcgagat gaacaaaaac acggtcgtgg
     1681 tcaccggcaa ggtgatcaac ggagacacgg gagaaatcat taccacggcc agcgaaacaa
     1741 aatccgacaa caaaatgaag ggcgactata aagccctgac ggaagaagtc tccaccaagc
     1801 tgatcacgaa gctcgtggat gaggtgctgg aaaagtggtc gaaagaactg gccaacaccg
     1861 ccaccgtcaa gctgattgtt acgggcctgg atgaagacac gctggaagat ttcaaagccg
     1921 aactgggcga agaaatcaag ggcgtcaagg acgtgaacga acgtttttac gcgggcggca
     1981 aggcggagtt tgatctggaa gtggaaggaa acgcccagga cgtggcccgc gatcttcgga
     2041 aaataaccgt caacaaacgt aaaataaaaa tcaaaagtag aagccaaaac agagttgagg
     2101 cggttttatt gccttaaaca tcggatattc ggaaactttt taagtgggga ggattgaatg
     2161 aaaagcagaa aagcatacat ggcgattgct tgtgcggtgg tcctgctgat gttcgcggcc
     2221 tgcgtgggga caaatgaaag cctgaagcag gggcaggagt ttgccagtca aaaccgctgg
     2281 gatgaagcga tgatttattt ccaaaaggcg gtcagcgagg aaccggacaa ccaggaattc
     2341 aaaaatgagc tggcccgggc caaacaggaa gtcgccaaaa gccgtctggc aagggccaaa
     2401 cagtcctatg cggcagccgg acaaaatttc atggcgctgg agcgactgtc caaagaagcc
     2461 gatgaaattt tgaaactgga cccgaacaat ccggaaagca agtctttttc cgcaacggtc
     2521 aacgggcagg tcaacaacct gaaaacctcc ctgaaaaatc tgtatcagca ggcggaagcc
     2581 gacatgcaga aggaagactg gctggcggcc atcgacaaac tgaagcaggt caacgccatt
     2641 ttcccgaatt atgaagacac gggcaaccgg ctcaggcgct ctctgtccga aggcaccagg
     2701 gccatgtacc agcagggaac caaactggca aaacaggaag actggaaagg cgcggcggaa
     2761 agctttaagg cggtcatgga cgtcaatcca aaattcctgg atgcggccca gcagtatgcg
     2821 aatgccgtga aaaatgacaa tcccgcttac ttcacggcgg aagcggccaa agccgagagc
     2881 gccggaaagc tggacagggc cattgccatg tacgagaaag ccctggagta tccgcaggcc
     2941 gatcccaaac tgaacgacag tctggcggcg ctgaaaacga aagcgagcca gattttctta
     3001 aacgaatcca tcgaactcct gaaacaggac cggctttacg gctcctttaa aaagctggac
     3061 agggccggaa aatattcgac ggccctgcgc ggcgatcccg cttacgggga acacgcgaac
     3121 cgactttgcg aagccctgat gaaacgggcc aataaatttt cggaaaagga attgtgggga
     3181 aacgcgctgg tctggctgca gttgatcgac aaaatcaacc cggattacga aggattgttc
     3241 cagaagatga atgaagtgaa ggaccccatc tcccggcgtg tccggaagtc gatcgccgtt
     3301 tttgacttcg gctcccccag ttcagagaaa gacgcgggga aaatagccgc caaccgtctc
     3361 ctggcctatc tgcaccagaa cgccagcgtc gatttgcgca tcatcgaacg ggaaaacctg
     3421 cagtccatcc tgcgggaaat gcagctcagc cagacgggtc tggtggacat caaaacggcg
     3481 cagagcgtcg gcaagatgcg cggcatcgac acattcatca tgggagacgt gctgcagttc
     3541 tccgcaaagt acacggacaa tccgaccacc aaccaggtca aggttctggt caatgaagaa
     3601 gacgtccgca atccgaattt tgacgcctgg ctgcttcgca accccaaacc gtcggaggaa
     3661 gatctgaagc ttgcgccgcc catgacgacc aagaaaaagg actaccagtt cctgcccaac
     3721 aagcagggcg tcgcccgaat caacgccatg ctggaaatct cctacaagct ggtggacacc
     3781 cagtcgggtg aaattatcgc caacaacatt accggaaagc tggtcaagga agacaaatac
     3841 caggaaggcc ttgccatcgc gggcatcaaa gcggacccgc tggagctgcc cacggaaggt
     3901 gaagtgctgg atcagctggc caaagaaaag atcgcggaaa tgggccgcaa tgttctgaag
     3961 catttccaga gcttggaagt ggaatatttc aataagggtc aggacctgca gaagcgccgc
     4021 aattacgagg cggcgacgga aaaatacacg gatgccattt ttgatgaaaa attgaagacc
     4081 atctccacgc cgatttctca gaaagcggcg gagctgatcg aactgatcaa cgaattcaat
     4141 taatacggag accgatcttg ctgaagaaaa ttcttgccgg cgctgtttta ttcttgctga
     4201 tgctccttgc ggaaacagga ttcctccacg cacaggaaat caagctgtcc gtctttaact
     4261 tcgggacggc caacattgaa gcgtcggggc tgggaaccag cgtcaccaac gcgctgatca
     4321 gcaatctgag cagaaacccc tccatttcca tcctcgaccg caaagacctg gagacgtttc
     4381 tgaacctgaa cgaccttcag caaaacgatc agctggacaa cgtcgtcaat atcggctcgc
     4441 ggctggggct cgattttatt gtcgtcggct ccgtcgccaa gagaggatcc gccattaccg
     4501 tcaaatgcgg actggtccag atcgacaaga gaaaagaagt gtacagcacc caggttcggg
     4561 cgcagggcga atcggcgctg tccgcggaaa tcgccaagct tgcctccctg attacctcgg
     4621 tgctgaccaa gagcagcacg ggacctgaaa gcacgtccgc acccggcaca ggcggcggac
     4681 cgggcgccgg cgctcccgtc aatttcaagg gcgtcccggg caacaaaaaa atcatcctgc
     4741 gctggcagcc cgttcccggc ttcgcggccg ccggatatga agtctatcga gccctgaatg
     4801 cgaacggtcc gtttgccatg ctgggccaga cggataaaac ggaattcgcc gatcaaaacg
     4861 tggaaaacga catttcctat ttttacagaa tccgggccta cgaccggctg agccggttga
     4921 gcgattacac ccccgtcgtc tccgcccgca cggacttcgc gcccaatccg ccgatcattc
     4981 tgaagacgga agggcgcgcc aagagcatgc tgattgtctg gacacccaat cccgccaagt
     5041 ccgtggaccc ttcaccgctt gtcgggtata aaatttaccg ggccgccgaa gaagacggat
     5101 cctatcagga agtcaacaag ctgctgatca gcgatcttgc cgacagcggc gacggaaaaa
     5161 tctattaccg cgcaacggga ttgccggacg gacagacgtt cttctaccga ctggccgcct
     5221 acaacgccaa aggcattgaa agtgaattga gccaccccgt caaaggaacg acgctcccca
     5281 tcgtgtcttc cgtcaacgcc tccggccagt tgattcgtga agttaaatta acgtgggcag
     5341 gcatttcttc gcctttcctt accgcctaca atatttaccg ctccaccaaa agcgacggcg
     5401 gcttcttgaa aatcaaaaga atcagcgccg ccgagaacac cgcccggtac gagtattccg
     5461 acacggaagg cctgggcgac aacacccctt attattacac cgtcacggcg gaagatgatt
     5521 taaaggcgga aaccagtccg tccgcggtcg tcgccgcgac caccagggac attccgcccc
     5581 ggccggaaaa gttcaccgcc agaggcggcc tggtcaaaaa agtcgagctg acctggcagg
     5641 cggcgccgca gccggaagtg gaaggctatt acattttctg gtcgacggaa aaaaccggga
     5701 aatacgaact cctcaaaaag atccaggggc gcgaaaacaa ccgctacgtg gacgaatccc
     5761 gcggcttcaa ccccctggaa gacggaaccg cctattatta catgctgacc tcctacaaca
     5821 aggtggacgc gcaaagcaag caggctcagg ccggcgccac gaccaaaccc cggccgaaaa
     5881 aaccgaccgg gctgaaaggc gcctctaccc gcgtcaagga agcgcctctg acctggcagg
     5941 ccaatcccga aaaagacatt caggtttacc tgatccaacg ggccgaggga gacagggaca
     6001 acttctccag catcggcgag gccgccgcaa ccctctatac ggacgccaat ttaaaggacg
     6061 gcacgaccta tcgctaccgg attcaggcca aagacgcgga caacctgctg tcggacttct
     6121 ccgatgtgat tgtcgtgacc acgaaaccga ggccgcagcc gcccgagaac ctgcagggga
     6181 aatacgaatc cggaaaggcg gtcctgtcct ggacgcccaa caaggaagcg gacatttccc
     6241 attacatcat ctacgaaaaa aagtttatcg ggacggaaaa aatcgcggaa tcgaacaccg
     6301 cttcttatgc ggacggatcg ttgtctccgg ggaaaagcag gaattacgtc gtcaccgccg
     6361 ttgaccggag cggtctggaa agcgatgtca gcgcggagtt gaaggtgatc gccaaatgac
     6421 ggggcccctc cggccgcatc tggctgcaat caaatatccc tctgaccgca acattcattc
     6481 ggccggggct gtctttgcgc cggccgccgg aaaacatcaa aggaggaatc cacatgtctt
     6541 tactggccta tttaagcccg tcctggcgcg acgaggcgct tcaaagactt caaacggagc
     6601 tcaccccgga aaaaatgaac aacgtcacca cctccatgtc caacatttat aaaaactgcc
     6661 ccggcggcag cgaacagttt ctttttgtcg aatgcaagga cgggaaggtc acccgcgtcg
     6721 aaacgggttc gggcgagccg ccgcaggcgg agttccgcat tatcggcgac tacgaaacct
     6781 tcgcccggat ttcccgcgcg gagctgggcg cgcagaaagc gctgatgacg ggaaaactca
     6841 aactcaaagg caatctggcc cgcgcgctca ggctggccgc cgtcgccgac cgactgaata
     6901 aagttttgtc ccgcattcca acccaatatt aaagggagac aaccccatga ccagaattct
     6961 cgatccccag gatccctatt acatcgacaa ccccggccgc gtgaaagcga tccaggccga
     7021 aatggcccgg cgcggcattg acgtatatct cggctcgcgc atccgcacca tgagctttgt
     7081 catggacacc ttttgcccct ggcgcagcta cgtgatcatt ccgtccgatg ggcagcccgt
     7141 cttttttacg tttgtcgtgg acgccctgcg gattgccgac gaaacctgga tcgatcccga
     7201 ccgcgtccgc gcctacggtc cgatgggcgg catggaccag gtgtcggcga tcacggactt
     7261 catcaaagaa gagctcggca tccggaaggg ccgcctgggc tttgaagacg gcatctccac
     7321 ctacaccgcc gaaggaaatc tctcgcactg ggaatacacg cattttaaag acgcgcttcc
     7381 cgattttgaa tggatcaact cacatgacat cattgacgcc ctgtcgctca tcaaggacaa
     7441 gggcacgatc gagcgcttcc gcagagcgtc gctgatcgtg gatgaaggcc acaaagctgc
     7501 gcgggaagcc ctttccaacg gaggctacaa gggcatgacg gaaaccgtga tcgccggcat
     7561 tgccgcgctg gccatgcgca gggcgggcag cgtctccgaa tggaactttg cggggctcaa
     7621 tgaaattgca agcggcgtcc gcacgggact gggggcctgc accccgccca cgaccaaaga
     7681 aattcaggca ggcgagccgt tgatgctgga ttttcacgcc atgttcaaac tggcgctggg
     7741 cgaccattcg cacaattacc tcatcggacc cgccacccgg cggcagcgct ggcacgcgga
     7801 taattttgtc gccattattc aaaaagtgat tgacaactac cgggccggga caacccccgg
     7861 aacactggcc gccatcatga tggactttgc cgagtcccgc ggctgcggag acttcatcca
     7921 gcccgcctgc gaacacggca tcggtctttt cggcgacgag tggcgcatcg gcgccgtcgt
     7981 caatcccgac cttccctact ggacggatca ggatcatgtc tatcaggaaa acgagatggt
     8041 cgtctgcgcg atgcagtacg cctcaccgga cgagcatatc ggattccgct atgaaaacga
     8101 catcgtcatc acccgtgacg gctgcgaggt cctgtcgaaa tacccgctgg ggatcgagga
     8161 gatctcgtaa atcgcgacag atgcgagaat catgaaagcc tcccgtgttc cctggccgat
     8221 cgatctggcg ggttttttct ggcagcggaa aatcctgggc cgcagcattc ccctgctggc
     8281 aagcttcaaa gtcacccatc ggtgcaatct gacctgccgg gcctgcccct ttcacctccg
     8341 gccccacgcc gatatcagcc atatgtcccg ggacacagcc gtccgcgccc tggaaaccct
     8401 gagcagtctc ggaacgcgca tcgtcgtttt tgaaggcggg gagccgctgc tgtggcgcga
     8461 cggcaagcac cgcctgcacg atctggtgga ctgcgcccgc aggcatttcc tgcgtgtggc
     8521 ggtaaccacc aacggcacgc tgccgctgga cgctcccgcc gacacgctct gggtcagcct
     8581 ggacggatgg aaggaaacgc atgacgatct gcgcagcaat tcttttgacc gggtcctggc
     8641 tcatcttgaa aaaacaaaac acccgcgcgt ttttgttcac tgcacgatca atcgccgcaa
     8701 ctggaccgat ctggagagga tggcccgaca gatccgcaaa ctcccgaccg tgcggggcat
     8761 gaccttccag tttttttatc cctacggcct gggcgaagac gaccttgttt tagcgcccca
     8821 ggagcgtcgc gccgcggtcg ggacgctcct ggggctcaag aaaaagggct atccggtttg
     8881 tcagtcatcc acccggcttc gatccatgat caacaaccgc tggcgctgcc atgacgacct
     8941 gctggtcaat gtggatccgg acggttcgat tgttcagggc tgctacgtca aaaaccgcgg
     9001 aaccgtaaac tgcgacgcct gcggcttcac gccggttgcg gaagcctccg gggcgctgga
     9061 cctgatcccc ggttcgctgt gggcaggctg gaaactgttc ttgtgaaaac aggagcgatg
     9121 ggcgaaaaga gtgtcccggt gacccctccg gcaaactcag gacaggccct gccgaatgct
     9181 gacagtgaaa agccataagc gaagagtgga aatctccttg acaatagcct gaaatttaat
     9241 aaaaatcttt tcacgattca cgggattttc gctcagtctt tgacaacaaa ccagccaatg
     9301 atttgccggg ggcaagctca aggaggtcgg gtatgcaaaa aaggaatgcc ggacatgtgg
     9361 tcgcagccgt cctgttcatg atcctgatgg ttcccggagt ttcggcggcg caaagcgcca
     9421 gcaggcaaaa cggggcggtc agcaccagag aaacctgcta ccaatgccat ttccagatca
     9481 agtccctgaa ggaaggttcc aaacatgccg cgctctcctg cgatatctgc cacagcggcg
     9541 ccaaagagca tctgcagagt ttccggaaca agccggcaac ggccatcgat caggccgcct
     9601 gcggcaaatg ccacggcaac cagctcaaca gcttcatgag aatcaactat tcggcgcagg
     9661 cccgcaaaga aaaaggcgtg ccgacgggcc gctcaccgct gcaggaaaaa ctgctggcgc
     9721 cgcacggatt caccaaggag cacaatgaac cccgggcgca tgttttcatg gtcaccgacc
     9781 agtttgccgt ggatcgcttc atcggcggac gcttccagta taaaaaaggt ttggccggca
     9841 tccatcagac cggcaaggca tgggatgtcc tctatgacac gggccgcgaa ctgcccgaaa
     9901 ccgcaaaagc aggcaacgcc acctgcatca agtgcaaaac caccgatcac attctgcaat
     9961 ggaaatacat gggcgacaag gacccgaagg ccaggtggga caggagctcc gacatcgtcg
    10021 ccttctccaa agcgacgcaa aatcccatgg gctgcatcca ctgccatgac gcccatggcg
    10081 ccgcgccccg ggtggtccgc gacgcgctca tcgatgccat cgatcgcgac ggcgcccaaa
    10141 ccttcgcccg gaacggcaaa acggatctga aggtcgtcga tttccgggga ttccggaaaa
    10201 tcggcgtcat gagcaagacc gattcccgga tgatgtgcgc ccagtgccat gtcgaatacg
    10261 cgtgcaatac aggcttcgaa ttcgacagcg gtaaaaaagt tgactatgac gaccggcgca
    10321 ccaaccatta ccccctgaaa aacgccaacg atattcttgc ccactacaag aagctgaatt
    10381 attacgattt caaacacgcc gtgacgggcg caaggctcgt caaactgcag catccggagg
    10441 ccgaaaccta ctggggcagc gcccacgacc gggccggcgt ccagtgtcat cagtgccaca
    10501 tgccgaaagc caaggaccag ggaggaaaga tgtacaccaa ccacggcgtc attcgcccca
    10561 tccaatcgat taaagatgcc tgtctgggct gccatcccaa ttcctccgag gaagagaagc
    10621 actaccagat tgagacggcc caaaactaca ccagaggcaa aatgcgcaag gcggaatact
    10681 ggctggcgaa attaatcgac gcctacgagg ccgcccaaaa agccggcgtt ccggaagcga
    10741 cgcttgcgca ggcccgcgag aagcacgagg aagcgcatgt tttgtgggaa tggtggacgg
    10801 cggaaaacag cgacggctgg cacaacccac aactcgcccg ggagagctta accgcatcga
    10861 tcatcgcatc gaaaaaaggc gtggacatcc tgacccgggc cagggcaacc aa
//
LOCUS       DAOSRM010000076        10890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_5911_length_10890_cov_91.173973, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000076 DAOSRM010000000
VERSION     DAOSRM010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5911_length_10890_cov_91.173973"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLA58_07780"
     CDS             complement(<1..368)
                     /locus_tag="PLA58_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62730.1"
                     /translation="MSQYYKDAEDLYRIYGYFLDRVLKDEKIGTKMSKAGIIIKFMYT
                     DPDAEITIDLKNPPSKPGYYGSFYLGPCDLKEDVWSKQPADHSHRFWHGLENPIAGIT
                     KGIIKQGGKVTAMLKLLPVI"
     gene            complement(458..763)
                     /locus_tag="PLA58_07785"
     CDS             complement(458..763)
                     /locus_tag="PLA58_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62731.1"
                     /translation="MDNLDVLPDGSIWAGCHTKRLSFVAHSKDASKLSPSEVVRVLPL
                     KNGGYDVARVYQNDGKELSGSSVAASCKNRLLVGAIYENFFLDGTLPPGKSLEDARR"
     gene            complement(860..1141)
                     /locus_tag="PLA58_07790"
     CDS             complement(860..1141)
                     /locus_tag="PLA58_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62732.1"
                     /translation="MEIFSYRDGKLRYLETIKSPELTTPNDILGVGPRQFYVTIDHGW
                     TSKGGKMLENYLQIPVFHVLYYDGGQFKKVAGGIGYANGIAQSPDGKAV"
     gene            complement(1075..1563)
                     /locus_tag="PLA58_07795"
     CDS             complement(1075..1563)
                     /locus_tag="PLA58_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62733.1"
                     /translation="MKKIIILVCAVVVLIVVFLVKTLWNAGELKRIEPFSLYSFVAVP
                     GFPGVEDIDIDRGAAVALGSFTDRRAAIAGQKRPTSGILAYDLAAPGAGPVLLKTDFP
                     GHLTPTESIYITGPTAANGSSSSTWVRPVIFLKPQAFAPWRFFPIATESCAIWKPSKA
                     RN"
     gene            complement(1603..2763)
                     /locus_tag="PLA58_07800"
     CDS             complement(1603..2763)
                     /locus_tag="PLA58_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR62734.1"
                     /translation="MDFTLNEEQKMLVDTIRTMGQREKFKELARHIDETGEFPYDLLK
                     KYADLGLLGMTISAEHGGGGQPALNAILCIEELAKFSPIIAGPVFESNVGPVRVIDIF
                     GTPEQKKAIIPGVCGGELSVSVCMTEPEAGSDLTALTTHADEDGDGYILNGRKCFISG
                     GGHASHYLVYTRFGGVKGYKGIGGLLVEKGSPGFTFGKQEKFMGLRGMPSCDLIFDNV
                     RVPKKNLVIAPGEFGKLMWTFDIERCGNASMCLGIAGGAFEEAKAYAMERKAFNRPIC
                     EFQAVQFMMADMAAKIDAARLLVYRAVSGAGQGLPSIYEASMAKAYANEMVVDVTSTA
                     MQVFGGYGYSMEFPVERMYRDAKAWGVAGGTIQMLKIGIAGMIFGRRFDQRK"
     gene            complement(2790..4157)
                     /locus_tag="PLA58_07805"
     CDS             complement(2790..4157)
                     /locus_tag="PLA58_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOR62735.1"
                     /translation="MEYTQQLIRFCSDLSFDTLPEQVWHKAKLCVLDYVANIYGSLEL
                     DAVRSSIEFVRSLGDSEAATALGCGFKTGLQSAAFINGLTGEAIEAQDGLRFGGNHSG
                     VSVIPAALAVAETLELTGKRLIEAVVAGYEVSNRVAAAMHPWHTLSGFLPTGTCGTLG
                     SAVAVSKLMGQAYPVMLNAIGIAGYILPVSMAEQLMGGYTVKIVQGGQAAGAGIMAAG
                     LAGKGITGSPFVLEGTELKGGITQITMKVDAKPERITERLGEHYSINDVYFKPYTACR
                     HTHGAAQATLALKKQKGFVPDDVESVEVFTYGVAALAVGKGVPEGGSFVSAQFSIPHV
                     VAACILDGTLGPAQLTERRIVDPAIIALRQKVTVKTDDELNKMYPDKTASRVEILLKS
                     GERLVKTVDIPKGDPRDPMEAGDIAQKVKFFAGSRDQNKVDRIVDTILNLEKVNNIKE
                     LASFI"
     gene            complement(4218..5018)
                     /locus_tag="PLA58_07810"
     CDS             complement(4218..5018)
                     /locus_tag="PLA58_07810"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOR62736.1"
                     /translation="MSNHPNDYIKPELMACCGAREIRDGDIVVVGTGFPAMSANIARY
                     THAPKATLMQESGVFDARPARPALSVGDPCLNPGAAMIGGLTDVMGMFLQGGWVDVGF
                     LAGSQIDKYGNINTTAIGDYEKPKSRLPGSGGANPIGALAKKVLIIALHNTRSLAEKV
                     DFITTPGYLDGPGARERWGLPTDTGPQVIITNKAVLRFDATTKEAYLESFHPGASVDE
                     VVKLTPWDLRVSDDVHETEPPRAEEIRVIREVIDPHRMITIYEKRGYV"
     gene            complement(5034..5969)
                     /locus_tag="PLA58_07815"
     CDS             complement(5034..5969)
                     /locus_tag="PLA58_07815"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOR62737.1"
                     /translation="MGKVMSTADAIQQFVHDGEFLFIGGYVCRPPFAAIHEIIRQKKK
                     DLTITRSNAADDFDMLIGAGCVKRFIATFISLGVYGLARCYRRSIEKGIPHKIELEEY
                     TNLSLPLMLMAGAMGMPFVPVKDMVGTDLMNIKSFMGDGKYKMIESPFDGSPVMLAPA
                     LHPDVAIIHVQQADEEGNAQMWGIGGDCKWGANAARKVIVSCERIVSRETIGKDPSRT
                     IVPAFKVVAVTEEPFGAHPGYTPGFYDMDLAYGYLYKEASNTVEGFQAFLDEWVFGVK
                     DRTEYVNHYIQKFGYAQYKKLQAKFDYGYPVSYTY"
     gene            complement(6025..8406)
                     /locus_tag="PLA58_07820"
     CDS             complement(6025..8406)
                     /locus_tag="PLA58_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HOR62738.1"
                     /translation="MSTQQALSQPAEPSRIQMLRWRIIDAPQEVCIERARCLTESMRL
                     HWDKPPLTRMSLALEHILKNISVIIRENEIIVGCRTSKLKGAPLFPENKSLWIEGDLE
                     NFDRRALQRALITQAEKDELATRILPFWKGKTVEEQMQRSMPEDILTDMDKYIFTMML
                     EITYGIGHFTMNHDKILEGGLSSVIGEAGQKLAGLSAEDQKGEKGLFYEAVIRSCRAV
                     IAFAHRYSERALAMAAAESDPGRKAELQEMARVCARVPEHPAETFHEAVQSLYFIHLV
                     SQIESGGNSISLGRIDQILYPYYRRDAEAGRITQAQARELTAMLFVKVNEIWNVLEEA
                     FIPGGEGTEGKTTQNVTVGGVGVDGKDAVNEMSYIALDAFADVRTVQPNFGVRVSPDC
                     PVELMRRAVDYTRDGVLMHFFNDEAIIRSLVHAGHTLEDARNYGMVGCLEPNAQGKTF
                     GSTFAVQFSGIKCLELALANGIDNIFGYPSGVETGDPAAFENFEDVWSAYDRQVRHFI
                     NQMVRGMEVLDRTIAENVPSPFASAMVDGCLEKGLDLTRGGAHYNSTGVQLMGFANVV
                     DSLYSVKKAVFEDKLFTMADLASWLSQDWQDAEDKRAYFLNRVPKYGNDNDDVDALGV
                     RVMDHYCDVLSGYRNFRGGFFWPGVFSVGFHITMGAFTGATPDGRFAGDVLGNGITPT
                     TGNAVSGPTAIMNSVVKLPVTRATNGVNLNMRFQGKKIRTEHLAALIQTYFRNGGTQV
                     QFNMVDSEVLRDAQQHPEKHRDLFVRVSGYSAEFVGLSEIAQDEIISRTEFEI"
     gene            complement(8426..9256)
                     /locus_tag="PLA58_07825"
     CDS             complement(8426..9256)
                     /locus_tag="PLA58_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR62739.1"
                     /translation="MGQMVVITGLADGMGRHVAKMLAKSGHSVAGFDMDAKGIASLAS
                     ELKDIGGDHLLETIDITDRPAILKFRDQVLARYGRVDTVLSNVGIGFFSPFEEVQLEK
                     ALKCLEINVIGAAAIFQAFLPSMRERRAGKLIAMSSLVGRIPFPFESIYSASKFAVTG
                     MMVSMKYEVEPFGIKVALIEPAQVSTTFAAKIHVLPPENSPYRDRARRFIERDNELIK
                     TAPTPPVAAEKIVKVIEAEKPALFNQIDFMSSFFLWLNQFLPKSVRDAILVNHMRIKV
                     "
     gene            complement(9290..10282)
                     /locus_tag="PLA58_07830"
     CDS             complement(9290..10282)
                     /locus_tag="PLA58_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOR62740.1"
                     /translation="MEFKGITLVTGAAGFMGSHLVEFLAAKGVRVRASARPRKDTSFF
                     DRLGVEYVPADLTKPETLPGLFEGEVDRIFHLGAICNFSTPYESLASTNVEGVKRITQ
                     LAMKKGVKRYVHVGSTSVYGYYQGTPFREDSPRNPQDSYGRSKRDGEDVVWGRIKEGF
                     PAVITRPCTVYGPRCNDGAGKAFSRPSSITAIPGSGRQLLSNVRAEDVAAAVYHLSLT
                     DAAVGQAYNLAEDTHPTLEEALKMASEAFGAKPPTTHLPLWLVKIVAKFDGMASARKG
                     RIPDLELDAVKYLYADYVVDNSKLKKTGYRMIYPDFRQSMKQIGEEYRVNLGGR"
     gene            complement(10321..>10890)
                     /locus_tag="PLA58_07835"
     CDS             complement(10321..>10890)
                     /locus_tag="PLA58_07835"
                     /inference="COORDINATES: protein motif:HMM:NF025908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOR62741.1"
                     /translation="AKRKRRMSIKYAILGLLQYSDMHGYRIKAHIEKNFGHMWSINFG
                     QIYPSLKQLQEEGLVNMLEIAPSENGGPRKKLYSITPKGVKELSKWLTEPTGKSILIR
                     DPFLLKFAFFGFGEDANALTIIDEQIEFFESQLKRRQTNRKKWERQGVYVRLLAELGV
                     TQNQMYLEWLHHAKKEVEKNMKRARREKA"
BASE COUNT         2190 a         3321 c         2933 g         2446 t
ORIGIN      
        1 ttgatgaccg gcagcagctt cagcatggcg gtgacctttc cgccctgctt gatgatgcct
       61 ttggtgatcc cggcaatcgg attttccagg ccgtgccaga accggtggga atggtcggcc
      121 ggctgcttgg accacacatc ctccttgaga tcgcaggggc ccagatagaa ggagccgtaa
      181 tagccgggct tcgacggcgg gtttttcagg tcaatcgtga tttccgcgtc cgggtccgtg
      241 tacatgaatt taatgatgat gcccgccttg ctcatcttcg tgccgatctt ctcgtctttc
      301 agcacccggt ccagaaaata tccgtaaatt ctgtagagat cctccgcatc cttgtaatac
      361 tggctcattg ccgcctcctt atatcactcg ttaaaagtcg aagagaagtc cgcgctgcaa
      421 ctctcccgga tcaaccaatc gaacgaaacg ggcaacgtca ccgcctcgca tcctccaggc
      481 tcttgccggg cggcagcgtg ccatcgagaa agaaattttc gtagatcgct ccgaccagca
      541 gccgattctt acaggatgcg gccacactgg aaccggacaa ttctttcccg tcgttctgat
      601 agacccgcgc tacgtcgtag ccgccgtttt tcaaaggcag cacgcggacc acctcggacg
      661 gggaaagctt ggacgcatcc ttcgagtggg ccacaaacga caaccgtttg gtatggcatc
      721 cggcccagat cgacccgtcg gggagaacgt ccaggttatc cacgccgctg ttgagttcga
      781 tgaccccctt ctcggccagg tcgcccgttt gaatattgcg tgcataaaca tgaatctttc
      841 gtccaacagt ggctccccct tacactgctt tgccgtcggg gctctgcgcg atgccgttgg
      901 cgtaaccgat gcctcccgcg acttttttga attgtccgcc gtcgtaataa agaacatgga
      961 aaaccggaat ctgaagataa ttttccagca tctttccccc cttcgaggtc cagccgtgat
     1021 cgatggtcac atagaactga cggggaccca ccccgagaat atcgttcggc gtcgtcaatt
     1081 ccgggctttt gatggtttcc agatagcgca gctttccgtc gcgataggaa aaaatctcca
     1141 cggtgcaaac gcctgtggtt tcaaaaaaat gacgggcctc acccatgttg atgacgaaga
     1201 gccgtttgcc gccgtcgggc ccgtgatata aatggattcc gtgggcgtca gatggcccgg
     1261 gaagtctgtt ttcaaaagga cgggcccggc gccgggcgct gcaagatcat aggccagaat
     1321 gccggaggtt ggacgctttt gaccggcgat ggccgcccgg cggtcggtga acgatcccag
     1381 cgccacggcc gcgccgcgat cgatatcgat atcctcgacg ccgggaaatc cagggacggc
     1441 gacaaaactg tagagggaaa agggttctat ccttttgagt tcgccggcgt tccagagcgt
     1501 cttcacgaga aacaccacaa tcagaacaac aacagcacaa acaagaataa tgattttctt
     1561 catgctttgt tcctttctgt tcgggcctcc ggaaaaggct ttttatttgc gctggtcgaa
     1621 gcgcctgccg aagatcatgc cggcaatccc gatcttcagc atctgaatcg tgccgcccgc
     1681 caccccccag gccttcgcgt cgcggtacat gcgctccacg ggaaattcca tgctgtagcc
     1741 gtatcccccg aacacctgca tggccgtgct cgtcacgtcc accaccattt cgttggcgta
     1801 agccttggcc atcgaggcct cataaatcga cggcagcccc tgccccgcgc cgctgacggc
     1861 ccgatacacc agaagcctgg ccgcgtcgat tttcgcggcc atatccgcca tcatgaactg
     1921 caccgcctgg aattcacaga tcggccggtt gaaggccttt ctttccatgg cgtaggcttt
     1981 tgcctcctca aacgcgccgc ccgcgatccc cagacacatc gaagcattgc cgcatcgctc
     2041 gatatcgaaa gtccacatca gcttgccgaa ctcgcccggc gcaatcacca ggttcttctt
     2101 cggaactcgc acgttgtcaa agatgagatc gcaggagggc atgccccgaa ggcccatgaa
     2161 tttttcctgc ttgccgaacg tgaagccggg cgatcccttc tccaccagca ggccgccgat
     2221 gcccttgtac cctttcacgc cgccgaagcg ggtatagacc aagtagtggc tcgcgtgtcc
     2281 gccgccggag atgaagcatt tgcggccgtt gaggatataa ccgtcgccgt cctcatcggc
     2341 gtgcgtcgtg agagcggtga ggtcggagcc cgcttccggc tcggtcatgc agacggacac
     2401 gctgagttca cccccgcaga cacccgggat gatcgctttt ttctgctccg gggttccgaa
     2461 aatatcaatg acccggaccg gcccgacgtt ggattcgaag accggccccg caatgatagg
     2521 gctgaatttg gccagctcct cgatgcacag aatcgcgttg agagccggct ggccccctcc
     2581 gccatgctcg gccgagatcg tcatgcccag gagcccgaga tccgcgtact ttttcaacag
     2641 gtcgtacggg aattcccccg tttcatcaat gtgccgggcc agttccttga atttttcccg
     2701 ctgtcccatc gtccgaatgg tatcgaccag catcttttgt tcttcattta atgtaaaatc
     2761 catgttcatt ccttagctaa agtattggtt tagataaaag aagctaattc ttttatatta
     2821 tttacctttt ccagattcaa aatcgtatcg acgattcgat ctaccttgtt ttgatcccga
     2881 ctgccagcga aaaacttcac tttttgcgcg atatctcctg cttccatcgg atcgcgcgga
     2941 tcccctttcg gaatgtccac cgttttcacc agacgctcgc cgcttttcag caggatttcc
     3001 acacggctcg cggttttgtc cggatacatt ttattcagtt catcatcggt tttcaccgtc
     3061 accttctgtc gcagcgcaat gatcgccgga tcgacaattc gccgttccgt gagctgcgcg
     3121 ggacccagcg tcccgtcgag gatgcaggcc gccacgacat gtggaatgga aaactgcgcg
     3181 gagacaaaac tgccgccttc cggaacgccc ttccccacgg ccagagcggc cacgccgtaa
     3241 gtgaacacct cgacggactc cacgtcatcc gggacaaacc ctttttgttt tttcagggcg
     3301 agcgttgcct gcgccgcgcc atgcgtgtgc cggcaggcgg tgtaaggctt gaaataaacg
     3361 tcgttgatgg aataatgctc tcccagcctt tccgtgatac gctccggttt ggcgtccact
     3421 ttcatggtaa tctgcgtgat gccgcccttg agctccgttc cttccaaaac aaacggactg
     3481 ccggtaatcc cctttcccgc gagtcccgcc gccatgatgc ccgcgccggc cgcctggccg
     3541 ccctggacaa tcttcacggt atagccgccc ataagctgct ccgccatgga aacgggcaga
     3601 atatagcccg caatgccgat ggcgttgagc atcacgggat aggcctgccc catcagtttg
     3661 gaaaccgcca cggccgaacc gagtgtgccg caggtgccgg tgggcaaaaa tcccgacagg
     3721 gtgtgccagg gatgcatggc tgccgctaca cggttggata cctcataacc cgccactacc
     3781 gcctcgatca gacgcttgcc ggtcagctcc agagtttccg caacggccag cgccgccgga
     3841 atgacggaca cgcccgaatg attgcctccg aagcgaagcc cgtcctgcgc ctcgatggcc
     3901 tcgccggtca gtccgttgat gaaagccgcg ctctgcaatc cggtcttgaa tccgcagccc
     3961 agcgccgtcg cggcttcgga gtcgcccagc gaccggacga attcaatcga cgagcgcacg
     4021 gcatccagct ccagcgatcc gtaaatattg gccacatagt ccagaacaca gagttttgcc
     4081 ttgtgccaca cctgttcggg cagggtgtca aaagacagat ccgaacaaaa tcggatcagt
     4141 tgttgcgtgt actccatatt tcatctcctc ctttaaaaaa cggaatgtcc cgggcgggaa
     4201 caaccggtta aacgctccta cacatatccg cgcttctcgt agatcgtgat catccggtgc
     4261 ggatcgatga cttcccggat aacgcggatt tcttcggcgc gcggcggctc ggtttcatgg
     4321 acgtcatcgc tcaccctgag gtcccacggg gtcagtttga ccacttcgtc caccgacgcc
     4381 ccggggtgaa agctttcgag ataggcctct ttggtcgtcg catcaaaacg aagaacggcc
     4441 ttgttggtga tgatgacctg cggaccggtg tccgtcggaa gcccccaccg ctcccgggcg
     4501 cccggaccgt ccagataacc gggcgttgtg atgaagtcca ctttttccgc caggctgcgc
     4561 gtgttgtgaa gcgcaataat gagaaccttt ttggccagcg ctccgatggg gttcgcgccg
     4621 ccggagccgg gaaggcggct tttcggcttt tcataatcgc cgatggcggt ggtgttgatg
     4681 tttccgtact tgtcgatctg gcttccggcc agaaatccca catccaccca tccgccctgc
     4741 aaaaacatcc ccatgacatc cgtgagcccg ccgatcatcg ccgctccggg gttcaaacag
     4801 ggatctccca cggaaagcgc gggccgcgcg ggccgcgcgt caaacacgcc cgattcctgc
     4861 atcagcgtag ctttgggcgc gtgcgtgtag cgggcgatgt tcgccgacat ggcgggaaaa
     4921 cccgttccca cgaccacaat gtccccgtcc cggatttccc gggcgccgca gcacgccatc
     4981 aattccggct tgatataatc attgggatga ttgctcatgc gtccacctcc tcttcagtac
     5041 gtgtaggata cggggtagcc gtagtcgaat ttcgcctgca gctttttgta ctgcgcgtat
     5101 ccgaattttt gaatgtagtg attgacgtac tctgtgcggt ccttcacccc aaacacccat
     5161 tcgtccagaa acgcctgaaa tccctcgacg gtattggaag cttccttgta cagatagccg
     5221 taggccagat ccatgtcata gaagccgggc gtgtatccgg gatgcgcgcc gaagggctcc
     5281 tcggtcaccg cgacaacctt gaacgccggc acgatggtgc gggacggatc cttgccgatc
     5341 gtttcccggc tgacgatccg ttcgcaactc acgatgacct tacgcgccgc gttggcgccc
     5401 cacttgcagt cgccgccgat gccccacatc tgggcatttc cctcctcgtc cgcctgctgc
     5461 acatgaatga tcgccacgtc cggatgcagc gccggcgcca gcatcacggg gctgccgtca
     5521 aagggagatt cgatcatctt gtacttgcca tctcccatga aagatttaat gttcatcaga
     5581 tcggtcccca ccatgtcctt gaccggcacg aagggcatgc ccatcgcgcc cgccatcagc
     5641 atgagcggaa gggaaagatt ggtgtattcc tccagctcga ttttgtgggg gatccccttc
     5701 tcgatcgatc gccggtagca acgggcaagg ccgtacaccc ccagcgaaat gaaggtggca
     5761 atgaaccgct tcacgcagcc cgcgccgatc agcatgtcga agtcgtccgc ggcgttgctc
     5821 cgcgtgatgg tcaggtcctt ctttttctga cggatgatct cgtggatggc cgcaaacggc
     5881 ggtctgcaga catagccgcc gataaacaga aactccccgt catggacaaa ttgttgaatg
     5941 gcgtccgcgg tggacatcac cttgcccata cccctcctcc tttgcgtgaa acggcggcgt
     6001 ttccgccttc aaccgacccc gctgtcagat ttcaaattct gttctgctga taatttcatc
     6061 ctgggcgatt tcgctgaggc ccacaaattc cgcggagtag ccgctgaccc ggacaaacag
     6121 gtcgcggtgc ttctccggat gctgctgcgc gtcccggaga acctccgaat ccaccatatt
     6181 gaactgaacc tgcgttccac cgtttcgaaa ataggtttgg atcagcgccg ccaggtgttc
     6241 cgtccggatt ttcttcccct gaaagcgcat gttgagattg acgccgttcg tggcccgggt
     6301 caccggaagt ttgacgacgg aattcatgat cgccgtcgga ccggacacgg cgttgcccgt
     6361 cgtgggcgta atgccgttgc ccaggacgtc tccggcaaac cggccgtccg gcgtggcgcc
     6421 cgtgaaagcg cccatggtaa tgtggaagcc gaccgaaaac acgcccggcc agaagaagcc
     6481 tccccggaaa ttccggtagc cggacaacac gtcgcagtag tggtccatca cgcggacgcc
     6541 cagcgcgtca acgtcgtcgt tgtcgttgcc gtacttggga acgcggttca ggaagtaggc
     6601 gcgtttgtcc tctgcgtcct gccaatcctg cgacagccac gaggcaaggt cggccatcgt
     6661 aaagagtttg tcttcaaaga ccgccttttt cactgagtag aggctgtcca ccacattggc
     6721 aaaccccatg agctgcacac ccgtggaatt gtagtgtgcg cctccccgcg tcaggtccag
     6781 acctttttcc agacagccgt ccaccatggc cgaggcgaag ggcgagggaa cattttcggc
     6841 aatcgtgcgg tcgagcactt ccatgccccg gaccatctgg ttgataaaat gacgcacctg
     6901 cctgtcatag gcgctccaga cgtcttcaaa attttcaaag gccgccgggt ctcccgtttc
     6961 gacgccgctc ggatacccga agatattgtc aatcccgttg gccagggcaa gctccagaca
     7021 tttgatgccg ctgaactgca ccgcaaaggt ggatccgaag gttttccctt gcgcgttcgg
     7081 ctccagacag ccgaccatcc cgtagttgcg ggcgtcttcg agggtgtggc ccgcatggac
     7141 cagcgaccgg ataatcgctt cgtcgttgaa aaaatgcatc agcacgccgt cgcgggtata
     7201 atcaaccgcc cggcgcatca gctccacggg acagtccggc gaaacgcgga cgccgaaatt
     7261 gggctgaacc gtccgcacgt cggcaaatgc gtccagggca atgtagctca tctcattgac
     7321 ggcgtctttc ccgtcaaccc ccacgccgcc cacggtaacg ttctgcgtcg ttttgccttc
     7381 cgtcccctcc ccgccgggga taaaagcctc ttccagcaca ttccagatct cgttcacttt
     7441 tacaaaaagc atggccgtca gttcccgggc ctgcgcttgt gtaatcctgc ccgcttccgc
     7501 gtctctccga taataggggt acagaatctg gtcgatccgg cccagggaaa tggaattccc
     7561 tcccgattcg atctgggaaa ccaggtgaat gaaatagagg ctctggaccg cttcatgaaa
     7621 cgtttcggcc gggtgttcgg gaacgcgggc gcagacgcgg gccatttcct gaagctccgc
     7681 tttccggccg ggatcggatt cggccgccgc catggcgagc gcccgttcgc tgtaacggtg
     7741 cgcgaaggcg atgaccgccc ggcagctccg gatcaccgcc tcatagaaga gacccttttc
     7801 acctttttgg tcttccgcgg aaagaccggc aagcttttgc ccggcctctc cgataacgga
     7861 tgacagcccg ccttccagga tcttgtcatg gttcatggta aaatgaccga tcccgtacgt
     7921 aatctcgagc atcatggtaa agatgtactt gtccatgtcc gtcagaatgt cttccggcat
     7981 gcttctctgc atctgctcct cgacggtttt ccccttccag aagggaagaa tccgggtcgc
     8041 cagctcatcc ttttccgcct gcgtaatcag cgcacgctgc agagcgcgcc ggtcgaaatt
     8101 ttccaggtct ccctcgatcc acagcgactt gttttccgga aacaggggtg cgcccttgag
     8161 cttggatgtg cggcacccga caatgatttc gttttcgcgg atgatgacgc tgatgttttt
     8221 cagaatatgc tccagggcga ggctcatcct cgtcagaggc ggcttgtccc aatgaagcct
     8281 catggattcg gtcaggcagc gggcgcgctc gatgcagact tcctgcggcg cgtcgatgat
     8341 cctccagcgg agcatttgga tgcgggaagg ttccgccggc tgcgacaggg cctgttgtgt
     8401 gctcataaaa cacctcctgc aaacgttaaa ccttgatgcg catatggttg accagaatcg
     8461 cgtcccggac cgacttcgga agaaactgat tgagccacag aaaaaagctg ctcatgaaat
     8521 cgatctgatt gaagagtgcc ggcttttccg cctcgatgac ctttacgatc ttttcagccg
     8581 caacgggcgg cgtgggcgcc gtcttgatca gttcattgtc ccgctcgata aaccgccggg
     8641 cgcgatcgcg gtaaggagaa ttctcgggcg gcagaacgtg gatcttggcc gcgaacgtcg
     8701 tggatacctg cgccggttcg atcagcgcca ctttgatgcc gaagggctcc acctcgtatt
     8761 tcatggagac catcattccg gtaacggcga atttgcttgc ggaatagatg gattcgaagg
     8821 gaaaaggaat gcgccccacc agcgacgaca tcgcgatgag tttgcctgcg cggcgctcgc
     8881 gcatggacgg aagaaacgcc tgaaaaatcg ccgccgcgcc gatgacgttg atttcgaggc
     8941 acttgagcgc tttctccagt tggacttcct cgaaggggct gaagaatccg atgcccacgt
     9001 tcgacagcac cgtatccaca cggccgtacc gggcgagcac ctgatcgcga aattttagaa
     9061 ttgccgggcg gtcggtgatg tcgatcgttt ccagcaggtg atcgccgccg atgtccttca
     9121 actctgacgc cagtgacgcg attcctttcg cgtccatatc aaaaccggcc accgaatgac
     9181 cggatttcgc gagcattttg gccacatgtc gtcccatccc gtccgccaag ccggtaatca
     9241 ccaccatctg tcccatgccc gcacctcccg ttgattcagt ctcttcgggt taccttcctc
     9301 cgaggtttac gcgatactct tctccaatct gcttcatgga ttgacgaaaa tccggataga
     9361 tcatccggta tcccgttttc ttcagtttgc tgttgtccac cacgtaatcc gcataaagat
     9421 actttaccgc gtccagctcc agatccggga tgcgcccctt gcgcgcagag gccattccat
     9481 cgaacttcgc gacgattttg accagccaga gcggcaaatg tgtcgtgggc ggtttggcgc
     9541 cgaaggcctc cgaagccatt ttcagggctt cctcgagcgt cggatgcgtg tcctcggcca
     9601 gattgtacgc ctgcccgacg gcagcatcgg tcagggatag atgataaacg gcagcagcca
     9661 cgtcttccgc gcgcacgttg gaaaggagct gccggccgct tcccggaatc gcggtgatgc
     9721 tggagggacg cgaaaaagcc tttcccgcgc cgtcattgca gcggggaccg tacacggtgc
     9781 agggccgcgt gatgaccgcc gggaagccct ccttgatgcg gccccagacg acgtcctcgc
     9841 cgtcgcgttt gctcctgccg taggaatcct gcggattccg ggggctgtct tcccggaagg
     9901 gagtcccctg gtaatagccg tacacgctgg tggaaccgac atgcacatag cgtttgacgc
     9961 ctttcttcat cgcaagttgt gtgattcttt tgacgccttc cacgttggtg gaagcaaggg
    10021 attcgtaggg agtggaaaaa ttgcagatcg ccccgagatg aaaaatccgg tccacctccc
    10081 cttcaaacag ccccggcagc gtttcgggtt tggtgaggtc ggcgggaaca tattcaaccc
    10141 cgagacggtc gaaaaatgat gtatccttgc ggggacgggc ggacgcccgg acccggactc
    10201 cttttgcggc cagaaattca acgaggtgac ttcccataaa tccggcggcg ccggtaacca
    10261 gggtaatgcc tttaaattcc atgatcccct cgactgaagt tgatcacaaa caaggtaatt
    10321 tcaggctttt tctcgacgag ctcgtttcat atttttttct acttcttttt tggcatggtg
    10381 gagccattcc aggtacatct gattctgcgt cacgccgagt tcagccagga ggcggacata
    10441 cacgccctgc cgctcccact ttttgcggtt ggtctgccgg cgtttcaatt gggactcgaa
    10501 aaattcgatt tgttcgtcga tgatggtgag ggcgtttgca tcttctccga atccgaagaa
    10561 agcaaattta agcagaaacg ggtctcggat gaggatggac tttccggtcg gttcggtgag
    10621 ccatttcgac agctccttca ctcccttggg cgtgatggaa taaagttttt tccgggggcc
    10681 tccgttttcc gacggcgcga tttccagcat attcaccaat ccctcttcct gcaactgttt
    10741 caggctggga taaatctgtc cgaaattgat cgaccacata tggccgaaat ttttttcaat
    10801 gtgcgccttg atccggtaac cgtgcatatc cgaatactgc aggagcccaa gaatggcgta
    10861 tttgatcgac atccgtcttt tcctctttgc
//
LOCUS       DAOSRM010000077        10640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_6071_length_10640_cov_92.063581, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000077 DAOSRM010000000
VERSION     DAOSRM010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10640
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6071_length_10640_cov_92.063581"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLA58_07840"
     CDS             complement(<1..745)
                     /locus_tag="PLA58_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR62742.1"
                     /translation="MASLWIDERDIKFQLHEVFNIGETLLGKGRYADHDVEMCNMVLD
                     QAQKFAEVEIAPTYPDEVHRKPVEAVFKDGKVFAPEAYHRLYKLYCEGGWMTTADSPE
                     VGGQAFPFIIGAACANMFLAANQAFLMYPGLTHGAARLVEDHFQHPLRDIIIEKMFSG
                     VWAGTMCLTEPGAGSDVGILKTSAKKNADGTYSITGTKCFISAGDHDLTENIIHPVLA
                     RIEGDPPGTKGISIFIVPKIRINEKGELGE"
     gene            complement(897..1283)
                     /locus_tag="PLA58_07845"
     CDS             complement(897..1283)
                     /locus_tag="PLA58_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOR62743.1"
                     /translation="MAVEKGQTFSGREKVDKYRPIYYAGASGDFNPIHIDPEFGKMVG
                     LGGAILQGLCTLAFASKTVTDWVGDPGKMKKLKCRFSAPVLMEDTISIQGTVTDVQSG
                     RARIDLKVVNQHGVEVLQKVEAEVEA"
     gene            complement(1294..1731)
                     /locus_tag="PLA58_07850"
     CDS             complement(1294..1731)
                     /locus_tag="PLA58_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOR62744.1"
                     /translation="MAIDTKFVGKTYPSYTYEVGKEKIKEYAKAIKNLDPHYLDDDFA
                     AKSKYGAIIAPPTFAVVFGAYLIEPVFNDKDLNLNMAMLVHGEQELEFLEVVKAGDAV
                     TTGAKIISIQNKEKLDVIAIEVQSRNQHGRDVCRGIYTFVVRK"
     gene            complement(1792..2964)
                     /locus_tag="PLA58_07855"
     CDS             complement(1792..2964)
                     /locus_tag="PLA58_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR62745.1"
                     /translation="MNYDFTPEQLSIKDNFTKFCAKEIEPRASLLDEAPHEEVPKLIK
                     ANIKKMAGIGYLGMGHEEAYGGTNLDLISQAIAGEAVAGACASTFLSCGASSGLFGMP
                     LRLFGSEEQKKKYLPGVIKGEIIGCFGLTEPEAGSDAASIKTTAVKDGDKWILNGTKT
                     FITNATIADVAAVFAYNDRAKGPGGGVTCFLVEAGTKGFRAGKPFDKMGFRGSPTAEL
                     ILEDCEVPDSAVLGAVGSGFIQAMMTLEYGRIGMATVCLGIASKCLELANKYSKERVV
                     FGKPINRFQEISFKLADMLIMSDTARLLIYQAAWAKETNHPESAVLASVAKLWASETG
                     KQISDLAVQIHGGYGYMKEYPVERLYRDARLGEIGEGASEIQRILIAKDLIRKYAA"
     gene            complement(3015..4166)
                     /locus_tag="PLA58_07860"
     CDS             complement(3015..4166)
                     /locus_tag="PLA58_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR62746.1"
                     /translation="MEFGFTEEQLMFKDSVYKFAKKEIVPLVEEADLKSEFSMEVWRK
                     LGEMGILGLPFPEELGGSGASVETCCLAGEALGHAGVDQGHLLALGAHTYLCADTIFK
                     HGSPEQLKKYIPKLAGGEWIGCMGLTEPGAGSDAASITTTAVKKDGKWILNGTKTFIT
                     NAPVCNVCVVYATVDKALKHKGITAFIVERGFPGFATGAPFHKMGVRASATSEVILDN
                     CEVPEENLLGEVGRGFEYTHETLSWDRSSLLAPFVGALQFAIETCAKYSQDRVQFGRP
                     INAFQAIQHKLADIKMIKEAAKMVVYRVAHDKDSGKPLNHLHTSVAKSIVGDYGIKAA
                     SDAVQIFGGYGFIHEYPIERFLRDVKLAQIGGGTSEMQKFIISRILSFM"
     gene            complement(4187..4795)
                     /locus_tag="PLA58_07865"
     CDS             complement(4187..4795)
                     /locus_tag="PLA58_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR62747.1"
                     /translation="MNERNKNDLLNMRRAQLTAAAYKVVAQKGYNDFTIKDIAEEAGL
                     STGLVHYYFKNKEDLLFKLLKEMNANLRDSLKKALEELSEPRDKLVAFCDEAFALVEK
                     EKAYFYVLIDFWAQINHDARIRQANVKLFQSYRAEIAAILQEGVAKGVFTVEDVKLTS
                     VIIIALIQGTIIQYAIDSEGFNYREIKEQIKEQILSKIVNGK"
     gene            complement(4842..5744)
                     /locus_tag="PLA58_07870"
     CDS             complement(4842..5744)
                     /locus_tag="PLA58_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62748.1"
                     /translation="MAAKESPTVEINPFKRILKMPGALCGGISTGSDKIRSGYGNGDC
                     LFFDFEHLVFAVADGTERFPWASRDLLQRLAERLSRSGSPETARDWKDMMNNEIYAGQ
                     KYQHKTTFSAVSLRREKEAVTLIIANGGDSVVTVMDGLTAKIRRQTGRNMEFAGRSRE
                     IVEVMEHRVSDQNVRVLLSTDGFDDVWRFCLRRSLVGSAREVLERVGLDGISEEIFGI
                     LEGQRGRFEYDDVGFILLDPNVVKRVKGKALIMGGTRPFEEECYRQQYTPQVYDRWIP
                     DAQWDEQEEMLAGAGIRVLKAGPC"
     gene            complement(5744..6208)
                     /locus_tag="PLA58_07875"
     CDS             complement(5744..6208)
                     /locus_tag="PLA58_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOR62749.1"
                     /translation="MVEKEHIIIESGDAVQPFAYSVGMHGSRFFAEIRDNKRFLGVKC
                     PRCGKVYIPPRGVCGDCFVEMKEWVEVGPKGVIGTFTILRYAFIDPETGAQKPVPYGY
                     GFIRFDGADTLFQHYINIEDETKIRVGARVEPVFARERKGSIRDIEYFKVID"
     gene            complement(6229..7386)
                     /locus_tag="PLA58_07880"
     CDS             complement(6229..7386)
                     /locus_tag="PLA58_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62750.1"
                     /translation="MAKRVAIVGTGQTFHKSHRPDVNGQELINEAVLRALQDADLKMK
                     DIDAIVIGNMDHFEGINYVDCWSVDGSGGTMKPIIKLTTGGTTGCTVAIGGYHLVGSG
                     MFDRVLIIGWEKNSESDTTGAITTAFDPIWDRLVFAGAISGLAAEAQAYMARYGASDR
                     DGARVSVRDRKHAMNNPHAQLRKEVSLEEVLASPMLADPIHLLDVCPRTDGACAVIMA
                     SEDVAEKICPKPDWIWGTAARHSYSYLGDADYGRLTSMREGSQELWKKAGIKEPRKEL
                     DVVEMYQPYSFGGLIWIEDMGLVGPGEAPQYIWDGNTDMSGELPINPSGGVISCNPIG
                     ATGLIRCAEAAMQVMGKAEGRQVPDVNFAFSSGFGGCWWTDMILHGKNKPK"
     gene            complement(7398..8543)
                     /locus_tag="PLA58_07885"
     CDS             complement(7398..8543)
                     /locus_tag="PLA58_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOR62751.1"
                     /translation="MDRAAIIGVGMTKIEKRKISQTFADMAWEAVNKALTDAGMTIDD
                     IDNVVTTSSDFWDGRTISCMAVGDASGAAHKNISCVEGDGAYGAFYGMMRVLSGSYKT
                     TLVTGHTKGSESVSSLITNAAFDPIYERSLGMDMITACAMQARSYMHRTKTTAQQMAL
                     VSVKNHGNAFKNPLSQLPLKIAVQDVLQSEMIADPLHKLDCSPVSDGCAAVIIAHESV
                     AEKFKQQPVWIKGVSFCADSFFFGDRDLSHARSLAEAAKKAYAMAGIRDPKKEIDVAE
                     LYDAFTYQELLWLEEMGLADDATAGKLLEKGEFNIDGRLPVNASGGLLSGHPVIAAGL
                     YSMGAVVRQIRGDAGGFQVKKAKTGLVHGVNGLGGQSHCVFILDKDK"
     gene            complement(8560..9027)
                     /locus_tag="PLA58_07890"
     CDS             complement(8560..9027)
                     /locus_tag="PLA58_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOR62752.1"
                     /translation="MSDQFQGIEPMVYQSQINVPYSWWAGDTATRFFVTLRDEKKITA
                     TKCGDCGKVLLPPRKICPRCFTENMEWVKVSDEGTVLSFAVARRPFAAIPADKKVPVI
                     WGLIQLDGADTALLHYLDGLRPEDVSIGMRVTAVYSEARKGTIRDISHFKPVK"
     gene            complement(9088..9858)
                     /locus_tag="PLA58_07895"
     CDS             complement(9088..9858)
                     /locus_tag="PLA58_07895"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOR62753.1"
                     /translation="MADYNAMELMICVAARNLENGATVVVGTGAPCAAAMLSQKTHSP
                     NLTIMFEAGGVAPILPSMPISVGDSRTFHKAILASSMPDIMETCQRGMVDYTFLGGAQ
                     IDMYGNINSTMIGANHAKPKVRFPGSGGANDLASLCWRTMVMTPQDSKRFTDKIDFIT
                     SPGYLSGGNSRYEAGLPKGTGPYRIITNMAIMGFDDTSKRMKVLNINPGYNRTDIQDN
                     CGFELLWADRITETEPPHEEELRILREEVDPHRYIIGR"
     gene            complement(9870..>10640)
                     /locus_tag="PLA58_07900"
     CDS             complement(9870..>10640)
                     /locus_tag="PLA58_07900"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOR62754.1"
                     /translation="QGKKNLAFAGHTATHDMQILVGGECIDRLDIAYVIGLEARGLSP
                     NARRAVESGKIKLTEWTNATLSWRLKAAAMGLSYLPARNIMGTDTFKYSAAKEVLCPF
                     TNQKFAAFPALYPDFAAIHVHECDVYGNAHVFGSTVSDQDLAKASKRVVITTERIISN
                     DEIRRQPTATFIPFWCVDAVVEVPYGSYPGNMPYEYFSDEEHIRQWLKIEKDPEEFRK
                     FFKKQILDTKNFYEYLELNGGIARMLKLRAIEHLTHNR"
BASE COUNT         2087 a         3213 c         2835 g         2505 t
ORIGIN      
        1 gttcgcccag ctcgcccttc tcgttgatgc ggatcttggg aacgatgaaa atggaaatgc
       61 ccttggtgcc gggaggatcg ccttcgatgc gggcgagaac cggatggatg atgttttcgg
      121 tcagatcatg gtcgccggcg gaaatgaagc acttggtccc ggtgatggag taagtgccgt
      181 cggcgttctt tttggccgag gttttcagga tgcccacgtc ggagccggcg cccggctcgg
      241 tcagacacat cgtgccggcc cagacaccgg agaacatctt ttcgatgatg atatcccgca
      301 ggggatgctg gaagtggtct tccaccaggc gcgccgcgcc gtgggtcaga ccggggtaca
      361 tgagaaaagc ctggttggcg gccaaaaaca tattggcgca ggccgccccg atgataaacg
      421 gaaaagcctg gccgccgact tcgggcgaat ccgccgtcgt catccagccg ccttcacaat
      481 acagcttgta gagacggtga taggcttccg gcgcgaaaac cttgccgtcc ttgaacaccg
      541 cttcaacggg cttgcgatga acctcgtccg gataggtggg tgcgatctca acttcggcga
      601 atttctgcgc ctggtccagc accatgttgc acatctccac atcatgatcg gcataacggc
      661 ctttgcccag gagcgtttcc ccgatgttga aaacttcatg cagctgaaac ttgatgtccc
      721 tttcgtcaat ccacaaactt gccataccaa cctccttgtt ttcttttatt tatgaacgag
      781 tattcgttcc gagttctcct gaagatcgtg cggtaaggtt cttcgctgaa gttcatatgg
      841 caattgcggg taattgtcaa gcattcggaa acatttattc cgcaaaggaa cgaattttat
      901 gcttcaacct cggcttcgac cttctgaagc acttccaccc cgtgctggtt gaccaccttc
      961 agatcaatcc tggcgcgccc agactgcaca tccgtcacgg tcccctgaat cgaaatcgta
     1021 tcttccatca gcaccggagc ggaaaaccgg catttcagtt ttttcatttt gccgggatcg
     1081 cccacccagt cggtgacggt tttcgaggca aaggcaaggg tgcataatcc ctgaagaatc
     1141 gcccctccca gcccgaccat tttcccaaac tcgggatcga tgtgaatcgg gttaaaatca
     1201 ccggatgcgc cggcatagta aatgggcctg tatttatcga ctttctctct tccgctaaag
     1261 gtctgtcctt tttcaacggc catggcgcgc ctcctacttt ctgaccacaa aggtataaat
     1321 tccacggcag acgtcccggc cgtgctgatt tctggactgc acttcaatgg cgatgacgtc
     1381 cagtttctcc ttgttctgga tgctgatgat ttttgcgccg gtggtgacgg cgtctcccgc
     1441 cttcaccact tccaaaaact ccagttcctg ctcgccgtgg accagcatcg ccatgttcag
     1501 gttcagatct ttgtcgttga acaccggctc gatcagatag gcgccgaaga ccacggcaaa
     1561 agtgggcggc gcgatgatcg cgccgtactt cgattttgcg gcgaaatcgt catccagata
     1621 atgcggatcc aaatttttga tcgctttcgc atattctttg attttttcct tgcccacctc
     1681 ataggtatag gatgggtagg tcttgccgac aaatttcgtg tcgatggcca tgtgcatttc
     1741 tccttgaaat agggtcatgc ccccgaatgg aggactgctt tgcctgagcg tttatgccgc
     1801 gtacttccgg atcaggtctt tggcaatcag gatgcgctga atctccgaag cgccctcgcc
     1861 gatttcaccc agcctcgcgt ctcggtagag gcgctccacg gggtattcct tcatatagcc
     1921 gtagccccca tggatttgaa cggccagatc ggagatctgc ttgccggtct cggaagccca
     1981 gagtttggcc acggacgcca gcaccgcgga ttcgggatga ttcgtctctt tggcccatgc
     2041 agcctggtaa atgaggagtc tcgccgtatc ggacatgatc agcatatcgg ccagtttgaa
     2101 ggaaatctcc tgaaaacggt tgatcggctt gccgaaaacg acccgctcct tggaatactt
     2161 gttggccagt tccaggcatt tggacgcgat gcccagacaa acggtcgcca tgccgatccg
     2221 tccgtattcc agcgtcatca tcgcctggat gaagccgctg cccaccgcgc ccagaacggc
     2281 gctgtccggc acttcgcagt cctcgaggat cagttcggcg gtgggggagc cgcggaaccc
     2341 cattttgtca aagggtttac ccgcccggaa tcctttggtg cccgcctcca ccaggaagca
     2401 ggtcacgccg ccccccggcc cctttgcccg gtcattgtag gcaaaaaccg ccgccacatc
     2461 ggcgatggtg gcgttggtga tgaatgtttt ggtgccgttg agaatccact tgtccccgtc
     2521 tttcacggcg gtcgtcttga tggacgccgc gtcggagccg gcctccggct cggtcaggcc
     2581 gaagcagccg atgatttccc ctttgataac gccgggaaga tattttttct tctgctcttc
     2641 gctgccaaac aggcggagcg gcatgccgaa aagcccgctg gaggcgccgc aggataaaaa
     2701 ggtggaggcg caggcgccgg ccaccgcctc tccggcgatg gcctgactga tcaggtcgag
     2761 attcgtgccg ccgtaagctt cctcatgccc catgccgaga tagccgatgc cggccatctt
     2821 cttgatgttc gctttaatca gtttgggaac ttcttcatgc ggcgcctcat ccagcagcga
     2881 ggcgcgcggt tcgatttcct tcgcgcaaaa cttcgtgaaa ttgtccttga tcgacaactg
     2941 ttccggtgtg aaatcatagt tcatggcata ttctccttga atggatgtct ggttattctg
     3001 cttccgcccc ccggctacat aaagctcaaa atgcgggaga tgatgaattt ctgcatttcc
     3061 gaggttcctc cgccgatctg cgccagtttc acatctctca ggaaccgctc gatcggatat
     3121 tcatggatga acccgtagcc gccgaagatc tgcacggcgt cgctcgcggc tttgatgcca
     3181 tagtcgccga caatggattt cgccacggac gtatgcagat gattcaaagg cttgccggaa
     3241 tccttgtcgt gcgccacgcg gtaaacgacc attttggcgg cctccttgat catcttgata
     3301 tccgccagtt tgtgctggat cgcctggaaa gcgttgatcg gacggccgaa ctgcacccgg
     3361 tcctgggagt atttggcgca ggtctcgatg gcgaactgca gggcgccgac aaaaggcgcc
     3421 agaagggagc tgcggtccca ggacagggtc tcatgggtgt attcaaatcc ccggccgacc
     3481 tcgcccagca ggttttcttc cggaacttcg cagttgtcca gaatgacttc ggaggtcgcg
     3541 gatgcccgca cgcccatttt gtggaagggc gcgccggttg caaatccggg gaagcccctc
     3601 tcgacgatga aggcggtgat gcccttgtgt ttgagcgctt tgtccaccgt ggcatacacc
     3661 acacagacgt tgcagaccgg cgcgttggta atgaaagtct tcgtcccgtt gagaatccat
     3721 ttgccgtctt ttttgaccgc cgtggtcgtg atggacgcgg cgtccgaacc ggcgcccggc
     3781 tccgtcagtc ccatgcaccc gatccattcg ccgccggcca gcttcggaat gtattttttc
     3841 aactgttcgg gcgaaccgtg tttgaagatc gtgtccgcgc acaggtaggt gtgggcgccc
     3901 agggcgagca ggtggccctg atcgacgccg gcgtgcccca gagcctcgcc ggccaggcag
     3961 caggtctcca cgctggcgcc cgatccgccc agttcctccg gaaacggaag gcccaggatg
     4021 cccatctctc ccagcttccg ccacacctcc atggagaact cgcttttgag atccgcctcc
     4081 tcgaccagcg gcacaatttc tttttttgcg aacttataga ccgaatcttt gaacatcaac
     4141 tgttcttccg taaagccgaa ttccatgagt gcctccttat aatgccttat tttccgttga
     4201 cgattttgga gaggatctgc tccttgatct gctccttgat ctctctgtaa ttgaaccctt
     4261 cgctgtcaat cgcatactgg atgatggttc cctggataag ggcaatgatg atcacggacg
     4321 tcagtttcac gtcctctacc gtaaaaacgc ctttggccac gccttcctga aggattgccg
     4381 cgatctcggc gcggtagctc tggaaaagct tgacattcgc ctggcgaatc ctggcgtcat
     4441 gattgatctg cgcccagaaa tcgatgagta cgtaaaaata agccttttcc ttttccacca
     4501 gagcgaaggc ttcgtcgcag aaagccacca gtttgtccct gggctcggaa agctcctcga
     4561 gcgccttctt cagactgtcc cgaagattgg cgttcatctc cttgagcagc ttgaaaagca
     4621 ggtcttcctt gtttttgaag tagtagtgga ccagccccgt ggacaggccg gcttcctcgg
     4681 caatatcctt gatggtgaag tcgttgtacc ccttctgcgc aaccaccttg taagccgcag
     4741 ccgttaactg ggcgcgcctc atattcaaca aatcattttt attgcgttca ttcatctttt
     4801 cagcaacatt ttcctggcag cttgctgcgc tttgcagccg gtcaacacgg ccccgccttc
     4861 agcacccgga ttcccgctcc ggcaagcatt tcttcctgtt catcccactg tgcatcagga
     4921 atccaccggt cgtacacctg gggtgtatac tgctgccggt agcactcttc ctcaaaaggc
     4981 ctcgttcccc ccatgatcag agcttttccc ttcacccgct tcactacgtt cggatccaga
     5041 agaataaagc cgacatcgtc atactcaaaa cggccccttt gcccttccag aatcccgaaa
     5101 atttcttcac tgatcccgtc cagcccaacc cgttcgagga cttcgcgcgc ggagcctacg
     5161 agcgatcggc gcaggcagaa gcgccacaca tcatcgaatc cgtccgtgga aagaagcacc
     5221 cggacgtttt ggtccgacac ccgatgctcc atcacctcca caatctctct gcttcttccc
     5281 gcaaattcca tgtttcgtcc cgtctggcgg cgaatctttg ccgtgaggcc gtccatcacc
     5341 gttacaacac tgtctcctcc gttcgcgatg atcagggtta cagcctcttt ttcgcggcga
     5401 agggagacgg cgctgaaggt ggtcttatgc tggtactttt gaccggcata aatttcgttg
     5461 ttcatcatgt ccttccaatc ccgggccgtt tccggggagc cggaccgcga aagcctctcc
     5521 gccaaccgct gtaaaagatc gcgggacgcc caggggaagc gctccgtgcc gtccgccacg
     5581 gcgaagacca ggtgttcaaa atcaaagaaa aggcagtccc cgttgccgta gccgctgcgg
     5641 attttatcgc tgccggtgct gattcccccg cacagcgccc ccggcatctt caggatccgc
     5701 ttgaatggat tgatctcaac cgtcggcgac tcctttgcgg ccatcagtcg atgactttaa
     5761 aatactcaat gtcccgaatg ctgcccttgc gctccctggc gaataccggc tccacccggg
     5821 cgcccacccg gatcttcgtt tcatcctcaa tgttgatgta gtgctgaaac agggtatcgg
     5881 cgccgtcaaa cctgataaac ccgtagccgt agggaaccgg cttctgcgcg cccgtttccg
     5941 ggtcgataaa tgcgtagcga agaatcgtaa aggtgccgat gactcctttc ggacccactt
     6001 cgacccattc tttcatctcg acaaagcagt ctccgcagac gccgcgcggc ggaatataca
     6061 ctttcccgca tcgggggcac ttgaccccca gaaaccgttt attgtcgcga atctccgcaa
     6121 aaaacctgct tccgtgcatg cccacggaat atgcgaaggg ctgcaccgca tcgcctgatt
     6181 caatgatgat atgttctttc tcaaccataa tccctactcc tctttagatc acttcggttt
     6241 gttcttgccg tgcagaatca tatccgtcca ccagcaaccg ccgaaaccgc tgctaaaggc
     6301 aaagttgacg tccggaacct gccgcccttc cgctttaccc attacctgca tggccgcttc
     6361 cgcgcaccgg atcaggccgg tcgccccgat gggattgcag gagatgacgc cgcccgacgg
     6421 attgatcggc agttcgccgc tcatgtcggt gttgccgtcc cagatgtatt ggggagcctc
     6481 accgggaccc acaaggccca tatcctcaat ccaaatcagg ccgccgaaag aatagggctg
     6541 gtacatctcg acgacatcca gttctttgcg cggctccttg atgcctgctt ttttccagag
     6601 ttcctgcgaa ccttcccgca tgctggtcaa gcggccgtaa tcggcatcgc ccaggtagct
     6661 gtaggaatgc cgcgccgccg tgccccagat ccagtccggt ttggggcaga ttttttccgc
     6721 cacgtcctcg ctggccatga tgaccgcgca tgcgccgtcg gtgcgggggc atacgtccag
     6781 aagatggatc ggatcggcca gcatcggaga agccagcact tcttcaagac tgacctcctt
     6841 gcgaagctgc gcgtgcgggt tgttcatcgc gtgcttcctg tcgcgcacgc tcacccgggc
     6901 gccgtcgcgg tccgatgcgc catagcgggc catataggcc tgcgcctccg ccgccagtcc
     6961 cgatatggcg ccggcaaata ccaggcggtc ccagatggga tcgaaggctg tggtgatggc
     7021 gccggtggtg tccgactcgg aattcttttc ccagccgatg atcagaactc tgtcgaacat
     7081 tcctgagccg accagatggt atccgccgat agcgacggtg catcctgtgg ttccgccggt
     7141 ggtcagcttg ataatgggtt tcatcgtccc gccggaaccg tccacgctcc agcagtccac
     7201 gtagttgatc ccttcgaaat ggtccatgtt cccgatgacg atggcatcga tgtccttcat
     7261 cttcaggtcg gcgtcctgga gcgctctcag gacggcctcg ttaataagct cctgtccgtt
     7321 cacgtccggg cgatggctct tatggaaggt ctgtccggtt cctacaattg caactcgttt
     7381 agccatggtt tctcctccta cttatcctta tcaagaataa acacgcagtg ggactggcca
     7441 cccaggccgt tcacgccgtg caccaagccg gttttcgcct ttttcacctg aaaaccgccc
     7501 gcgtctcccc gaatctgccg gactaccgcg cccatgctgt agaggccggc ggcgatgacg
     7561 ggatgcccgg aaagcagtcc gccggaggcg ttcaccggca gccttccgtc gatgttaaac
     7621 tctccttttt ctaagagttt ccctgccgtt gcatcatcgg caagccccat ttcttcaagc
     7681 cacaaaagtt cctgataagt aaaagcgtca tatagttccg ctacatcaat ttcttttttc
     7741 gggtcccgga tgccggccat cgcataggcc ttcttcgccg cttcggcaag cgatcgggcg
     7801 tgagatagat cccggtcacc gaagaagaag ctgtccgcgc aaaacgaaac gcctttgatc
     7861 caaaccggct gctgcttgaa tttctccgca accgactcat gggcaatgat caccgccgcg
     7921 caaccgtcgg ataccggaga acagtccagt ttgtggagcg ggtccgcaat catctctgat
     7981 tgcagaacgt cctgaacggc gatcttcagg ggaagctgag acagcgggtt tttgaaagca
     8041 ttgccgtggt tcttcacgga taccagggcc atctgttggg cggtcgtctt cgtacggtgc
     8101 atgtaggagc gggcctgcat cgcgcaggcg gtgatcatgt ccatccccag gctccgctcg
     8161 tagatgggat caaaggctgc attggtaatg aggctcgaca cgctttcgga acctttcgta
     8221 tgccccgtca ccagcgtggt cttgtaagaa ccggaaagga ctctcatcat gccgtaaaaa
     8281 gcgccgtagg cgccgtcgcc ctcgacacag gagatgttct tgtgggccgc gccgctggcg
     8341 tctcccacgg ccatgcagga aatcgtccgg ccgtcccaga agtcgctgga ggtcgtcacc
     8401 acgttgtcga tgtcgtcgat ggtcatcccc gcgtcagtaa gggccttgtt cacagcctcc
     8461 cacgccatgt cggcgaaggt ctggctgatc tttctttttt caattttcgt cattccgacg
     8521 ccgatgatcg ctgctctgtc catagctgtg gctcctcccc tatttaaccg gtttgaaatg
     8581 cgaaatgtcg cggatggttc ctttccgcgc ctcggagtac acggccgtga cgcgcatgcc
     8641 gatgctcacg tcctcgggcc tgaggccgtc cagataatgc agcagggcgg tatcggcgcc
     8701 gtcgagttga atcagccccc agatcaccgg aaccttctta tccgcaggaa tggcggcgaa
     8761 gggccgcctc gccacggcaa aggaaaggac ggtcccttca tcggacacct tcacccattc
     8821 catattttcg gtgaagcagc gcggacaaat cttgcgcgga ggcagcaata cctttccgca
     8881 gtcgccgcat tttgtcgcgg tgattttctt ttcatcgcga agcgtcacaa aaaaccgggt
     8941 cgccgtgtcg cctgcccacc aggaataggg aacattgatc tggctttggt aaaccatcgg
     9001 ttcaatgcct tgaaattgat cggacatgat ttttctcctt aagggttgcg cccacccccg
     9061 cagacaccgc gagaggagca gattggccta tcttcctata atataccggt gcggatccac
     9121 ctcctcgcga agaatccgca gctcctcttc atggggcggc tccgtctccg tgatccggtc
     9181 ggcccagagc agttcaaagc cgcagttgtc ctggatatcc gtccggttgt agccggggtt
     9241 gatgttcagc accttcatcc gcttggacgt gtcgtcaaag cccatgatcg ccatattggt
     9301 gatgatccgg taggggccgg tccccttggg aagccccgcc tcatagcgcg aattcccgcc
     9361 gctcaaatac ccgggggacg taatgaaatc gatcttgtcc gtgaagcgtt tggaatcctg
     9421 cggcgtcatc accatggtcc gccagcacag ggacgccaga tcgttggcgc cgccggaccc
     9481 cggaaaccgc actttcggct tcgcgtgatt ggcgccgatc atggtggaat tgatgttccc
     9541 atacatgtcg atctgcgcgc cgcccagaaa ggtgtagtcc accatgccgc gctgacaggt
     9601 ttccatgata tccggcatgg agctggccag aatggccttg tgaaacgtcc gcgagtcccc
     9661 caccgagatc ggcatggacg gcagaatcgg cgcaacgccg ccggcttcaa acatgatggt
     9721 gagattgggc gaatgcgtct tttgcgacag cattgccgcg gcgcagggcg cccccgttcc
     9781 cacgacaacg gtggcgccgt tctccaggtt tcgcgcggcc acgcagatca tcagttccat
     9841 tgcattataa tccgccatag ttctcctcct tatcggttat gcgtcagatg ctcaatggcg
     9901 cgcagcttca gcattctcgc gatacccccg ttgagctcca ggtattcgta gaaattcttc
     9961 gtgtccagta tctgtttttt gaaaaatttt ctgaactctt cgggatcttt ctcgattttg
    10021 agccactgcc ggatatgctc ctcgtcggaa aaatattcgt aaggcatatt tccgggatag
    10081 ctgccgtagg gcacttccac aaccgcatcc acgcaccaga acggaatgaa ggtggcggtc
    10141 ggctgacgcc ggatctcatc gttggagatg atcctctccg tcgtgatcac cacgcgtttg
    10201 gacgccttgg ccagatcctg gtccgacacg gtcgatccaa agacgtgcgc gttcccgtaa
    10261 acatcgcatt cgtggacatg aatggccgcg aaatccggat acagggccgg aaaggcggca
    10321 aatttctgat tcgtgaaggg gcagaggact tccttggccg cgctgtactt gaacgtgtcg
    10381 gttcccatga tgttgcgcgc cggaagatag gaaagcccca tggccgcggc tttgagcctc
    10441 caggaaaggg tcgcgttcgt ccactccgtg agtttgattt tcccgctttc caccgcgcgt
    10501 cgcgcgttgg gggaaaggcc gcgcgcttcc aaaccgataa cgtaggcgat atccagccgg
    10561 tcgatgcatt ccccgccgac cagaatctgc atgtcgtgcg tggcggtatg gccggcaaac
    10621 gccagatttt tcttcccctg
//
LOCUS       DAOSRM010000078        10439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_6218_length_10439_cov_103.005297, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000078 DAOSRM010000000
VERSION     DAOSRM010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10439
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6218_length_10439_cov_103.005297"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1501
                     /gene="ffh"
                     /locus_tag="PLA58_07905"
     CDS             173..1501
                     /gene="ffh"
                     /locus_tag="PLA58_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOR62755.1"
                     /translation="MFESLSEKLEGIFKKLKGRGVLNEESVNAALREIRMALLEADVN
                     FKVVKDFIEEVRLRAVGKEVLESLTPGQQVVKIVHDRLVELMGGTSANIKFGSRIPAP
                     IMLVGLQGCGKTTTAAKLARLLSAEKRVYLVSADVYRPAAMEQLAVLGRQIKAGVFEA
                     GALRDPVKICTEALEQARREGYEVVLIDTAGRLHIDELLMQELRRIKESVNPAEILYV
                     ADAMTGQDAVNVGAKFNERIGIDGVIMTKMDGDARGGAALSLRAVVGKPLKFVGVGEK
                     IDALEVFHPERMANRILGMGDILSLVEKAQAAIDEKTARQMQESLRKSDFTLEDFRNQ
                     LKQIKKMGPLKDMIGMIPGMGKIKALKGMEPDDGELVKIAAIIDSMTKKERNNYLIID
                     GRRRKRIARGSGTTVQDVNRLLKNYIEIRKMMKKINSRGGVKALMRNFPF"
     gene            1565..1819
                     /gene="rpsP"
                     /locus_tag="PLA58_07910"
     CDS             1565..1819
                     /gene="rpsP"
                     /locus_tag="PLA58_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOR62756.1"
                     /translation="MAVKIRLTRMGAKGKPFYRIVAADKEYPRDGRFLEILGNYDPKK
                     NPAEITLKEDRVRDWLSKGAKPTLTVSHLLKTKGIAAAVK"
     gene            1948..2178
                     /locus_tag="PLA58_07915"
     CDS             1948..2178
                     /locus_tag="PLA58_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOR62757.1"
                     /translation="MKDLIKYISQALVDNPDQVEVTEVIGEQTSVIELRVAKEDLGKV
                     IGKQGRTAKAIRTILSATSAKVHKRAVLEIIE"
     gene            2181..2687
                     /gene="rimM"
                     /locus_tag="PLA58_07920"
     CDS             2181..2687
                     /gene="rimM"
                     /locus_tag="PLA58_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOR62758.1"
                     /translation="MDLFVFGEIVKTRGLHGCLKALSFLESSELLEGLTFAYLESPSG
                     RKSRHEIKKINSSGKFLFLEIKDIADVDAARAFVGCKLLLPREMLEPLPADEYYWHDI
                     IGLDVYTPAGRHLGRIESIFPTGAHDVYVCRGDAGEILIPATAGVVKQVDVAGKKMII
                     NLLEGLLP"
     gene            2684..3421
                     /gene="trmD"
                     /locus_tag="PLA58_07925"
     CDS             2684..3421
                     /gene="trmD"
                     /locus_tag="PLA58_07925"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOR62759.1"
                     /translation="MIRFDVLSIFPEMLQSPLEFSLLKKAREKGLIEVGLHNIRDWAQ
                     DKHRMTDDAPYGGGCGMVMKVEPVARAVAAVKQAGEASLVVLLTPQGEIFNQTIATEL
                     ARQKQIILICGRYEGFDERIREHLADREISLGDYILTGGELSALVVIDATARLIPGVL
                     GNDASASGESFSQGLLEYPQYTRPAEYSGWPVPEVLLSGNHARIERWQRIEALRRTWR
                     RRPELLEKTQLGEEDGELLEKIKQGRI"
     gene            3513..3860
                     /gene="rplS"
                     /locus_tag="PLA58_07930"
     CDS             3513..3860
                     /gene="rplS"
                     /locus_tag="PLA58_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009378341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOR62760.1"
                     /translation="MNVLEMIEKEQMRGDIPAFKTGDTVKVYVRIIEGQKQRIQAFEG
                     VVIRKRRGNSRANFTVRKVSYGVGVERTFPMHSPVIDRVEVVSRGVVRRSRLYYLRSL
                     RGKKARIKEAANN"
     gene            3889..4512
                     /locus_tag="PLA58_07935"
     CDS             3889..4512
                     /locus_tag="PLA58_07935"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOR62761.1"
                     /translation="MNIFERLAQVEGYQYVAGVDEAGRGPLAGPVVAAAVIFPPEYSN
                     SEINDSKKLTACKRDELYRIITEEAAAVGVGVVEADVIDRINILQASLQAMREAVLDL
                     LVKPDYLLIDGLHPIDFDVPQKTIVKGDSLSVSIAAASIIAKVSRDRIMEMYHRQFPQ
                     YNFLQNKGYGTAGHRKILREIGMCKIHRRSFHLKNKNLPQTALDWSE"
     gene            4509..4895
                     /locus_tag="PLA58_07940"
     CDS             4509..4895
                     /locus_tag="PLA58_07940"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOR62762.1"
                     /translation="MNELKDRRKIVTGKSGEGIAAGFLTQSGYRILDVNYRCPIGEID
                     IVAREKGELVFVEVKTRKSSALGYPEQAVGTAKQKKMSQLALWYLRDKKVGDAPVRFD
                     VVAILMLPSGNDIRLIRNAFDFIPFG"
     gene            complement(4941..6317)
                     /locus_tag="PLA58_07945"
     CDS             complement(4941..6317)
                     /locus_tag="PLA58_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOR62763.1"
                     /translation="MKFKKKILGIGAGYVGAPSMVMIASKCPDYKVTVVDINPERIAA
                     WNSDRLPVYEPGLDAIVRKTRGRNLFFSTDVEGQIQENDIIFVCVNTPTKTFGAGAGM
                     AADLQYWEKTARQILENSKTPKIVIEKSTLPIKTAQAMERILSSGKKNVTFDVLSNPE
                     FMAEGTAVSDLENPDRVLIGSRETRSGLAARAALVEIYTRWLPREKIITSNIWSAELS
                     KLVANAFLAQRVSSINSISALCEKADADISEVAHAVGTDSRIGAKFLKAGIGFGGSCF
                     KKDILNLVYLCRFYGLPEVAAYWESVVKINEYQQDRFIANILRSMFNTLAGKKICVLG
                     FAFKADTGDTRESPAIHITRKLLAEHAEVVISDPRALSNAAADLAHEKGVIRYVEDPY
                     KAATGCHALAVMTDWEAYRKLDYRKIYKSMVKPAFLFDGRNIVDHRKCFEIGFNVFPI
                     GKPALTRF"
     gene            complement(6343..7284)
                     /locus_tag="PLA58_07950"
     CDS             complement(6343..7284)
                     /locus_tag="PLA58_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR62764.1"
                     /translation="MKRILITGGAGFLGSHLCERLLSEGHDVICLDNFFTGHKNNIAH
                     LMGQAHFELIRHDIINPIYLEVDEIYNLACPASPVHYQFNPIKTIKTNVMGAINVLGI
                     AKRTRAKVLQASTSEVYGDPDVHPQQESYWGRVNPIGIRSCYDEGKRAAECLMMDYRR
                     QNAVNTKIARIFNTYGPRMAVNDGRVVSNFILQALQNRDITVYGDGSHTRSFCYAGDL
                     IDGLIRLMNTPDDFFGPVNIGNPGEFTILQLARLVIELTGSTSQIVFQPLPSDDPAQR
                     KPDIALAREKLGWQPTISLEKGLIGTIAYFKEILKTA"
     gene            7387..8241
                     /gene="rsmI"
                     /locus_tag="PLA58_07955"
     CDS             7387..8241
                     /gene="rsmI"
                     /locus_tag="PLA58_07955"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOR62765.1"
                     /translation="MDQETQGVLYMVATPIGNLEDITLRALRVLREVDLIAAEDTRRS
                     GILLSAYEIKTPMISLHEHNEKERSRVVLSRLRDGLSVAYVSDAGTPCVSDPGHHLVC
                     VALANGIRVVPVPGASALPAALSVCGFDAEPFLFCGFLPPRQNRRRRFLEAVSDEEYT
                     IIFYESPARIRETLEDALDILGDRQAVVARELTKVFEEIRQGRISDLLARASTAKSKG
                     EFTVMVRAAVRKPAPLSEEEIQATLLNIRSRKPVSLRDAVAEAARQTGMPRKKIYDAA
                     VKIRREFS"
     gene            8321..8920
                     /locus_tag="PLA58_07960"
     CDS             8321..8920
                     /locus_tag="PLA58_07960"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HOR62766.1"
                     /translation="MKVRLVFFALIMMLCAGFSACSSRSPETLKNDADQKAVKVVALL
                     PVENQTGDVRASKMLRLKISDELRFKGYPQVAIEVIDEGLAALAGGKEARKNDAVSPA
                     FIRETLGADAAMYCCLMESKAAKKIFYTPVTVSVRCELRSTATGETLWNAQGKSTGRS
                     FDLRGKTLDMKTAGDLEAVLEEVVGQVMESLPYGPKLRG"
     gene            8987..9754
                     /locus_tag="PLA58_07965"
     CDS             8987..9754
                     /locus_tag="PLA58_07965"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOR62767.1"
                     /translation="MSKGTKIIAAVLPVLLLLGFAEAQTPVTVKMTKGQAQVTSLTGK
                     VSMVCAGQTEAVDLKTYDFIKPGCEVSTGADGRLEMVLPDRSVVRFAQKTKFKLVQAD
                     IGARGSRSVGISMTLGKIWTNVRKSLVGGGDRFEISCQNAVAGVRGTIYRMDVERDQS
                     AVVKVYDGEVSVAGVSRTQQQAPAALGPPQPVAGPTVIEGPKPVSMEQWVYIVRSMQQ
                     IQITSDGRALKPRDFTEEEDMDDWVKWNKQRDKQFEQ"
     gene            9767..10324
                     /locus_tag="PLA58_07970"
     CDS             9767..10324
                     /locus_tag="PLA58_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOR62768.1"
                     /translation="MNIPNFLSLLRIILVPVFVIFVIQAEYDRALITFVIAGLTDALD
                     GTLARLLKCQTVLGAYLDPIADKLLLVTSFVMLAIFGLIPAWLAVVVISRDFMILLGI
                     AIFSLISIPYEIKPAIVGKVTTALQVATIFFALLYKAVTHDFGYYWITGLFWSTAFFT
                     FISGAVYILRGIRILNKSESEEAKK"
BASE COUNT         2399 a         2618 c         3140 g         2282 t
ORIGIN      
        1 tgaatgcctt ccttgccggg aaggccgcag gccttcaatt atgaacaaaa cgctcccaat
       61 ggccgctcaa tgggataaca tcgggcaaca gcccgttgag agaataaaaa gataccggtc
      121 tccgacagaa accgcgcttt ggagattcag acggcggata aaggtttgaa aaatgtttga
      181 aagtctttcg gaaaaactgg aaggtatttt taagaaactc aaaggccgcg gcgtcctcaa
      241 tgaggaaagc gtcaatgccg cgcttcggga aatccgcatg gcccttctgg aagcggacgt
      301 gaacttcaag gtcgtcaagg attttattga agaggtccgg ttgcgcgccg tcggcaagga
      361 agtgctggag agcctcacgc ccggacagca ggtggtcaag attgtgcacg accgcctggt
      421 ggaactgatg ggcggcacca gcgccaacat caaatttggc tcgcggattc ccgcgccgat
      481 tatgctggtc ggcctgcagg ggtgcggaaa aacgaccacg gccgccaagc tggctcggct
      541 gctgtccgcg gaaaaaaggg tttacctggt gtccgccgac gtttaccggc ccgccgcgat
      601 ggaacagctg gccgttctcg gccggcagat caaggccggt gtttttgaag ccggcgccct
      661 tcgcgatccc gtaaaaatct gcacggaggc tctcgaacag gccagaaggg aagggtacga
      721 ggttgtcctg atcgacaccg ccggccggct gcatatcgac gagttgctga tgcaggagct
      781 ccgccggatc aaggagagcg tcaacccggc cgaaattctg tatgtggcgg acgcaatgac
      841 cggtcaggat gcggtcaatg tcggggcgaa attcaacgaa cggatcggca ttgacggcgt
      901 catcatgacg aagatggacg gcgacgcgcg cggcggagcg gccctgtccc tgagggccgt
      961 tgtgggcaag ccgctcaaat ttgtcggcgt cggcgaaaaa atagacgcgc tggaggtttt
     1021 tcatccggag aggatggcca accggattct cggcatgggg gatattttgt cgctggtgga
     1081 aaaggcgcag gccgccattg atgaaaaaac cgcccggcag atgcaggaaa gcctgcgcaa
     1141 aagcgatttt acgctggagg atttccgcaa tcagcttaaa cagatcaaga aaatggggcc
     1201 cctgaaggac atgatcggga tgattccggg catgggcaaa atcaaagcgc tcaagggcat
     1261 ggagccggat gacggggagc tggtgaagat cgcggcgatt atcgactcca tgacgaagaa
     1321 agaacggaac aattacctga tcattgacgg ccgcaggcgc aaacggatcg ctcggggaag
     1381 cggaacaacg gttcaggatg tcaaccggct gttgaaaaat tacatcgaga tccggaagat
     1441 gatgaaaaaa ataaactccc ggggcggcgt gaaggcgttg atgaggaatt tcccctttta
     1501 aaatcaaatg aattatgata gaaaacaaag caattttttt gaaggaggtt tggagtaaaa
     1561 ctacatggca gtcaaaattc gattaacccg aatgggtgcg aagggcaaac cgttttatcg
     1621 tatcgtcgcc gcggacaagg aatatccccg cgacggaaga tttctggaga ttttaggaaa
     1681 ttatgatccc aagaaaaatc ccgcggaaat aacgttaaag gaagaccggg ttcgcgactg
     1741 gttgtccaaa ggcgcaaaac cgacgctgac ggtttctcat ttattgaaga caaaaggcat
     1801 cgccgccgcc gtcaaatagc cgggtggctg ccgatcgccg attaaacaat acaggattag
     1861 aacctcacat cgttgaactg aaaaagccgt aggaatgtgg aagcgaattt tcagatgcat
     1921 taaccaaaac cagtggaggg gctgacgatg aaagatctga ttaagtatat ttctcaagct
     1981 ttggttgata atcctgatca ggtggaagta acggaagtta tcggcgagca aacgtccgtc
     2041 attgaactgc gcgtggccaa ggaagatttg ggaaaagtca tcggcaagca gggaaggacg
     2101 gccaaagcca tccgcaccat tttgagcgcc acctccgcga aagtccacaa gcgcgctgtg
     2161 ctggaaatca ttgaataaaa atggatctgt ttgttttcgg ggagattgtc aaaacccgcg
     2221 gtctccacgg ctgtctgaaa gccctgtctt ttttggaatc atcggagctt ctggaagggc
     2281 tgacgtttgc ttacctggaa tcgccgtccg gccggaaaag ccggcatgaa ataaagaaaa
     2341 taaattcttc cggtaaattt ctctttctgg aaatcaagga tattgccgac gtggacgcgg
     2401 cccgggcgtt cgtcgggtgt aaactgcttc tgccgcggga aatgctggag cctctcccgg
     2461 cggatgagta ttactggcac gacatcatcg gattggacgt ctatacgccg gcaggccgtc
     2521 atctggggcg aatagaatcc atctttccca cgggcgccca tgatgtttat gtctgccggg
     2581 gagacgcggg agaaattctg atccccgcga cggccggcgt tgtaaagcag gttgacgttg
     2641 ccggaaaaaa aatgatcatc aatcttctgg aaggccttct gccgtgatcc gatttgacgt
     2701 tttgtccatt ttcccggaaa tgctgcaatc gcccctggag ttcagccttt tgaaaaaggc
     2761 ccgggaaaag gggctgatcg aggtcggcct gcataacatc cgcgattggg cccaggacaa
     2821 acaccgcatg acggacgacg ctccctacgg cggcggctgc ggaatggtca tgaaagtcga
     2881 gccggtggcg cgggcggtgg ctgcggtaaa acaggccggg gaggcctcgc tggtggttct
     2941 gctgacgccc cagggggaaa tcttcaacca gaccatagcg accgagcttg cccggcagaa
     3001 acagatcatt ctgatttgcg gacgatatga aggatttgac gagcggattc gggaacacct
     3061 ggcggaccgg gagatatcac tgggcgatta tattctgacg ggaggagagc tttccgcgct
     3121 ggtggtgatc gatgcgacgg ccaggctgat tcccggcgtg ctggggaatg acgcctccgc
     3181 gtccggggaa tctttttcgc aggggcttct ggaatatccg cagtacaccc gtccggccga
     3241 gtattccggc tggcctgttc cggaggtgct gctttccggc aaccatgccc ggatcgagcg
     3301 ctggcagcgg atcgaggcgc ttcgccggac ctggcgcaga aggccagaac tgcttgaaaa
     3361 aacacaactg ggcgaagaag acggggagct tttggaaaaa attaaacagg gccggatata
     3421 aaggcttgat tttcagacgg gtttggggta atgaaaccca cttttaaagg cagagtcatt
     3481 cgtatcttga aggttgcagg aggaacagga acatgaatgt attggaaatg attgagaaag
     3541 aacagatgag aggcgatatt cccgccttta aaacgggcga caccgttaaa gtatatgtgc
     3601 ggatcattga aggacagaag cagagaattc aggcttttga aggcgtggtc atccgcaagc
     3661 gccgcggcaa cagccgggcg aatttcacgg tccgaaaggt gtcctacggc gtcggcgtgg
     3721 aaagaacgtt ccccatgcat tcgccggtga ttgaccgcgt tgaagtggtc tcccgcggag
     3781 tggtccgccg ctcgcgtctg tattatctga gaagcctcag agggaaaaag gcaagaatca
     3841 aggaagccgc caacaactga tgccgtaaag acgggttagt ttttttcgat gaatatattc
     3901 gaaaggcttg cgcaggttga aggctatcaa tatgtcgcgg gtgttgacga agcaggccgg
     3961 gggccgctgg cgggcccggt ggtcgcggca gccgtgattt ttccccccga atattccaat
     4021 tctgaaatca acgattccaa gaaactgacc gcctgcaaaa gagacgaact ctaccggatc
     4081 atcaccgaag aggcggcggc cgtaggcgtc ggcgttgtgg aggcggacgt catcgaccgg
     4141 atcaacatcc ttcaggcgtc gcttcaggcg atgcgcgaag cggtgctgga ccttttggtg
     4201 aaaccggatt atctcctgat tgacgggctt cacccgatcg actttgacgt accgcaaaag
     4261 acgatcgtca aaggggattc actcagtgtg tccatcgccg ccgcgtccat catcgccaag
     4321 gtttcgcgcg accggatcat ggagatgtat caccgccagt ttccccagta caactttctg
     4381 cagaataaag gctacggcac ggccgggcat cgaaaaatcc tccgggaaat cggcatgtgt
     4441 aaaattcatc gccgttcctt tcatttaaaa aacaaaaatc ttccgcaaac cgcgctggac
     4501 tggtctgaat gaatgagctc aaagacaggc gaaaaatcgt caccggcaaa agcggagaag
     4561 ggattgccgc gggttttttg acgcaaagcg gttatcggat tctcgatgtc aattaccgct
     4621 gtcccatcgg tgaaatcgac atcgttgccc gcgaaaaggg cgaactcgtg tttgtggaag
     4681 tcaagacgcg aaaatcaagc gcgctgggct acccggagca ggcggtgggc acggccaaac
     4741 agaaaaaaat gtcgcaactg gcgctgtggt atctgcggga caaaaaggtc ggggacgccc
     4801 ccgtccgctt tgacgtggtg gccattctga tgcttccgtc cggaaacgac atccgcctga
     4861 tccgcaacgc tttcgacttt attccctttg gatagcaatc ctcatttcgc gcttttttgc
     4921 gtaagcggcg gcgtctgatc ttaaaagcgg gtcagggccg gtttgccgat cggaaacacg
     4981 ttgaagccga tctcaaagca cttccggtga tcgacgatat tgcggccgtc gaacaggaag
     5041 gcgggtttga ccatggattt gtagattttc cggtaatcca gcttccggta ggcctcccaa
     5101 tccgtcatga cggccagcgc gtggcaccct gttgcggcct tgtacgggtc ttcgacatag
     5161 cggatcactc ctttttcatg cgccagatcg gctgcggcat tggaaagcgc ccggggatcg
     5221 gaaatgacca cttcggcatg ctccgccagc agttttcgcg taatatggat ggccgggctt
     5281 tcgcgcgtgt cgccggtgtc ggctttgaag gcaaacccca ggacgcagat ttttttcccg
     5341 gccagcgtgt tgaacatgga tcgcaggata ttggcaatga atcggtcctg ctggtattca
     5401 ttgattttga cgacgctttc ccagtaggcg gccacttccg gcagaccgta aaaacggcac
     5461 aggtacacca ggttgaggat gtctttcttg aagcaggatc cgccgaagcc gatgcccgcc
     5521 ttgagaaatt tggcgccgat gcggctgtcc gtgcccacgg cgtgcgcgac ttcggagatg
     5581 tcggcgtccg ccttttcaca aagcgccgat atggaattga tggaggatac ccgctgcgcc
     5641 agaaaggcgt tggccaccag tttggaaagt tcggcgctcc agatgtttga ggtgatgatc
     5701 ttttctcgcg gcagccagcg ggtgtaaatt tccacaagcg cggcgcgggc ggccaggccg
     5761 cttctggttt cgcgcgaacc gatcaacacc cggtcgggat tttccagatc gctgacggcg
     5821 gttccctccg ccatgaattc cggattggaa aggacgtcaa acgtcacgtt tttctttccc
     5881 gacgacagaa tccgttccat ggcctgcgcc gttttgatcg gcagcgtgct tttttcaatg
     5941 acgattttcg gggtttttga attttcgaga atctgccggg cggttttttc ccagtactgc
     6001 aggtcggcgg ccatgccggc gcccgcgccg aaggttttgg tcggtgtgtt gacgcagacg
     6061 aaaatgatgt cgttttcctg aatctgccct tcgacgtccg tggaaaagaa aagattccgg
     6121 ccgcgcgttt tccggacgat ggcgtccagg ccgggttcat aaacgggaag ccggtcggag
     6181 ttccaggcgg cgatgcgctc gggattgatg tccacgacgg tcactttgta gtccgggcat
     6241 ttggaggcga tcatcaccat cgagggggct ccgacatagc ctgcgccaat gccgagaatt
     6301 tttttcttga atttcatgct gttccgtttc ctcccgcctt ggtcaggctg ttttcagaat
     6361 ttctttgaaa taggcaatcg tgccgatcag gcccttttcc agcgaaatcg tcggctgcca
     6421 tcccagtttt tcccgcgcca gggcaatgtc gggtttgcgc tgcgccgggt catcggacgg
     6481 caagggctga aagacgatct gcgatgtgct gcccgtcagt tctatcacca ggcgcgccag
     6541 ctgcaggatg gtgaattcgc cgggattgcc gatattcacc ggtccgaaga aatcgtccgg
     6601 cgtgttcatc agccggatca gtccgtcaat cagatcgcct gcgtagcaga aggagcgcgt
     6661 gtggctgccg tcgccgtaaa cggtgatgtc ccggttctgc agggcctgaa ggatgaagtt
     6721 gctgacgacg cgtccgtcat tgacggccat gcgcggtccg taggtattga agatgcgcgc
     6781 gattttcgtg ttgacggcat tttgccgccg gtagtccatc atcaggcatt ccgctgccct
     6841 cttgccttcg tcatagcagc tgcggatgcc gatgggattg acgcggcccc agtaggattc
     6901 ctgctgggga tggacgtccg gatcgccgta aacttccgaa gtagaagctt gaaggacttt
     6961 tgcccgggtc cgtttggcga tgcccaggac gttgatggcc cccatgacat tggttttgat
     7021 cgtcttgatg ggattgaact gatagtgcac gggcgaggcc gggcaggcaa ggttgtaaat
     7081 ttcgtccacc tccagataga tgggattgat gatgtcgtgg cggatcagtt cgaaatgcgc
     7141 ctgtcccatc aggtgggcga tgttgttttt atggccggta aaaaaattgt ccaggcagat
     7201 cacatcatgg ccttcgctca aaaggcgttc acacagatgg gagcctaaaa aacctgctcc
     7261 gccggtaatt aagattcgtt tcatgttgga tttgctgttc ttcctggtag tattttgttt
     7321 ttgtctctgc taataaagcc gaaggacaaa gatgtcaacc ataaaaagag tctgaaaggc
     7381 ggtgcaatgg accaagaaac acagggcgtt ctttatatgg tggccacgcc gatcggcaat
     7441 ctggaggaca ttaccctccg ggcgctgcgc gtgctcaggg aggttgacct gattgccgcg
     7501 gaagacaccc gccgttccgg aatcctgctt tccgcgtatg agatcaaaac gcccatgatc
     7561 agcctgcacg agcacaacga aaaggaacgc agccgggtgg tgctttccag gctgcgggac
     7621 ggactgagcg tcgcgtacgt ctccgacgcc gggacgccct gcgtctccga tccgggccat
     7681 catctcgttt gtgttgcgct ggccaacggc atccgggtcg tgcccgttcc gggagcttcc
     7741 gcgctgcctg cggctctgtc ggtctgcggc tttgacgccg agccgttttt gttctgcggt
     7801 tttctgcctc cccggcaaaa ccggcgccgc cgctttctgg aggccgtcag cgatgaagaa
     7861 tatacgatca ttttctacga gtctccggct cgaatccgcg aaaccctgga agatgcgctg
     7921 gacattctgg gagaccggca ggccgttgtg gcgcgtgaat tgaccaaggt gtttgaagag
     7981 attcggcagg ggcggatttc agacttgttg gcccgggcgt ccaccgcaaa aagcaaaggg
     8041 gaatttacgg taatggtgcg ggcggccgtc cgaaagcccg cgccgctttc ggaggaggag
     8101 attcaggcca cgcttttgaa cattcgttcg aggaaacccg tttcgctgcg ggatgcggtt
     8161 gcggaggctg caaggcagac cggaatgccc agaaaaaaaa tatacgatgc ggccgtcaaa
     8221 atccgccggg aattttcata aaagaaacgg gccgccgcga tttacatatt gcttgaagag
     8281 ttgtcatcac tatgctaatt ccacttcaaa aggagtgacc atgaaagtcc gtctggtttt
     8341 ttttgcactg attatgatgc tttgcgcggg ttttagcgcc tgttcgtcgc gctcgccgga
     8401 gacgctcaaa aacgacgccg atcagaaagc ggtgaaggtg gtggccctgc tgccggtgga
     8461 aaaccaaacc ggggacgtca gggcgtcgaa aatgcttcgc ctcaaaatat cggacgagct
     8521 tcgcttcaaa ggttatccgc aggtggcgat cgaagtcatc gacgagggcc tggcggctct
     8581 ggcgggcggc aaggaagcgc ggaaaaatga cgccgtttct ccggcgttca tccgggaaac
     8641 gctgggcgcg gacgccgcca tgtactgttg tctgatggaa agcaaggcgg caaaaaaaat
     8701 attttatacg ccggtcaccg tgtcggtccg gtgcgaactg cgcagcacgg caacgggcga
     8761 aaccctgtgg aatgcgcagg gcaaatccac cggcagaagt tttgacctcc ggggcaagac
     8821 gctggacatg aagacggccg gagatttgga agcggttctg gaagaggtgg tgggccaggt
     8881 catggaatcg ttgccgtacg ggccgaaatt aagaggatag cgcatcgcgg catttcagaa
     8941 aacaaaatca aaatctgttt ggggaaagac atcaaaggag aataccatga gcaagggaac
     9001 caaaatcatc gcggccgtcc ttccggtatt gctgctgctt ggtttcgccg aggcccagac
     9061 gccggttacc gttaaaatga caaaaggaca agcgcaggtt acgtctctga cggggaaggt
     9121 ttccatggtc tgcgcgggac aaacggaggc ggttgatctg aaaacctatg atttcatcaa
     9181 gcccggatgc gaagtgtcca cgggcgcgga cgggcggctg gaaatggttc tgccggacag
     9241 aagcgtcgtt cgttttgccc agaagacaaa attcaaactg gttcaggcgg atatcggcgc
     9301 ccgggggagc cgctccgtcg ggatttccat gacgctgggg aaaatctgga caaacgtgcg
     9361 caagtccctg gtggggggtg gcgatcggtt tgaaatttcc tgccagaatg ccgtggccgg
     9421 agtgcgcggc accatctacc gcatggacgt cgagcgggac cagtccgctg tggtcaaggt
     9481 gtatgacggc gaagtcagcg tggcgggtgt ctcccgaaca cagcaacagg cgccggccgc
     9541 cttggggcct ccccagccgg ttgccggccc gacggtcatt gaagggccga agcccgtctc
     9601 catggagcaa tgggtgtata ttgtccggtc catgcagcag attcagatca cgtccgacgg
     9661 ccgggccctg aaaccgaggg attttacgga agaagaggat atggatgact gggtgaaatg
     9721 gaacaagcag agagacaagc aatttgagca atagccggac gtgtaaatga atatccccaa
     9781 ttttctatct cttttgcgga tcatcctggt tccggtgttt gtcatctttg tgatccaggc
     9841 ggaatatgac agggcattga tcacttttgt cattgccggt ttgaccgacg cactggacgg
     9901 aacgctggcg agacttttaa agtgccagac cgttctgggc gcttatctgg atcccatcgc
     9961 cgataaactg ctgctggtca ccagttttgt catgctggcc attttcggcc tgattcccgc
    10021 ctggcttgcg gtcgtggtga tcagccgtga cttcatgatc ctgctgggaa tcgcaatatt
    10081 ctccctgatt tcgatccctt atgaaatcaa accggcgatt gtcggcaagg tcaccacggc
    10141 cctgcaggtg gccaccattt tttttgctct gctgtacaag gcggtgacgc atgacttcgg
    10201 atattactgg atcaccgggc ttttctggag cacggctttt ttcactttta tttccggcgc
    10261 cgtttacatt ttgcggggga taaggatact gaataaatcc gaatccgaag aggcgaaaaa
    10321 gtaagatatt tgcttgacac aaaagcgttt tactgctagg tcaccaagcg ctaaagttga
    10381 ggacaggcaa gtagctcagg gggagagcgc catcctgacg cggtggatgt ccagggttc
//
LOCUS       DAOSRM010000079        10421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_6229_length_10421_cov_99.901891, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000079 DAOSRM010000000
VERSION     DAOSRM010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10421
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6229_length_10421_cov_99.901891"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..422
                     /locus_tag="PLA58_07975"
     CDS             84..422
                     /locus_tag="PLA58_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOR62769.1"
                     /translation="MDGIIPEKAYFRIGEVSKILNVEPYVIRYWETEFKTVKPVRTKT
                     AQRLYRKKDVEELLTIRNLLYRERFTISGAKKQLLKIRGGREEPPEACSQDKLVLIKR
                     ELQQIKKMMS"
     gene            complement(467..745)
                     /locus_tag="PLA58_07980"
     CDS             complement(467..745)
                     /locus_tag="PLA58_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HOR62770.1"
                     /translation="MLCDTVKKGVECGFMTKKGCGFFGNSCHPVIEQCGDCNKIFEYE
                     IGRYCKVYPDPKSKWMTGNCPTASHVKIEIKEAQKTNPLKASKRASKK"
     gene            1008..1772
                     /locus_tag="PLA58_07985"
     CDS             1008..1772
                     /locus_tag="PLA58_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOR62771.1"
                     /translation="MKRGSLLKIFWRTFFIHATLNFRRMQNLGFAYAMIPLLQRRKIP
                     DKEVEGVLVRQLQMFNTNPYLAHPLVGSILKMEEDRREGEDPAPIMAVKQSLMGPYAA
                     IGDTFFWGALRPCAGICSAGLVWLGCAFAPLAFLLMYLPAHILVRWRGFFEGYSSGKH
                     GIECIRRMDLPGISGRVRWLALAALCAGSLWFLRVGYRGVAAEAPGSAAAAAMLAVIL
                     LCWWLVKRGVSQVYLLYGAAALAMIYSTRGLWLCWK"
     gene            1754..2029
                     /locus_tag="PLA58_07990"
     CDS             1754..2029
                     /locus_tag="PLA58_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOR62772.1"
                     /translation="MALLEMKTFTLKNKLGMHARAAALFVRIAQKYRSEIVIERNGQT
                     VNGKSILGILTLACPMGGQITVSARGSDADQALAELETLIENKFGEE"
     gene            2039..3823
                     /gene="ptsP"
                     /locus_tag="PLA58_07995"
     CDS             2039..3823
                     /gene="ptsP"
                     /locus_tag="PLA58_07995"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOR62773.1"
                     /translation="MMSDQGKKTFVLKGIGVSPGVVIGKVYRFDPLDAQISFYKLHSA
                     DMVPREIERFKNALKESSRQLLEIQENLRKTKVTEPLYIIDVHVLLLSDKKFINRTIK
                     YIRRLGVNAEWALRMTLDHYKQVFEGLEDVYISGRISDVQYVIQRILRNLSGEKREMV
                     WDVGQEGVVIVSHDLSPADTAQMKMDKIVGFATDSGGRTSHTAIVARSMELPAVVGLD
                     NVTRFVRTGDDIIVDGTSGLVVVNPYPDMLKRYEEKKRHYDEAKDEYLKYARQPAVTL
                     DRYAVKIGSNIEFVEEISSAVSHGAEHVGLYRTEFLYIYRDDLPTEEDHFNNYRQVVE
                     EKNFSWSTIRTFDLGGDKFTKYQKHAKEMNPQMGLRAIRFCLREVDIFKTQLRAIWRV
                     SALGKVKILFPMISCIEEIREAKRLLEEARRELVTQGVPVAERMEIGAMIEVPAAAVI
                     ADQLAKEVDYFSIGTNDLIQYSLAIDRSNERVTYLYEPLHPAVLRLIKRIVDAAHEAG
                     IRVAMCGEMAGDPLCCPILLGMELDELSMNHLAIPRIKRILQQSSLEESRKLLAQALS
                     YKSAAEVRAYVQNYMIHHFPDEFQKRDL"
     gene            3871..6492
                     /locus_tag="PLA58_08000"
     CDS             3871..6492
                     /locus_tag="PLA58_08000"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOR62774.1"
                     /translation="MSEKTPTEAGRQEEEYYSGCIYDEQDLWRFRPVKAYLSRVSIAD
                     ENVHALKELAGRGAVVYAIKQRSKLNSLILAEITARNGLPMPVYSHGVNMSFWQPVSK
                     MMKFLWSSFLRRFRQTALARQGKLDFLAAKVAARKSVIIHLGESEFIQSRAAEDAIAS
                     LMKLQAGLSFPVYIVPVLVAYGRRREKEDESLFNILFGQTEQTGTLRRLITFMRYSKQ
                     AFILPAEPVHLAEYLSANSHLTHNELVMDLRGELIDRIEEEKTSVVGPELKSREAVIG
                     MVMHDPKLNRFLDDYAQKSKKDRTALARDARRYLHEIASDYRETFVELWDKVLTWLWN
                     TVYDGVSIDSEGLANVRNLSKKMPFVIISCHRSHIDYLLLSYVFYKNNIQLPFIAAGN
                     NLSFFPMGYIFRRSGAFFLRRSFRGNEVYGEVFSKYMATLLKEGLPLEFFIEGGRSRT
                     GKMVMPKYGLLSMIIQAYQDKYCENLAAVPVYIGYDRVIEEKSYLQELSGAPKVQENT
                     VEIIKSTKILRKRYGRVYVNIGQPIILKDYLAAQEKSMDEMTVEERQSLYRKIGYEVV
                     LEINKVSVVTPFALVASGLLSHDRRGISHEELRGILNEFYEYLSTRQVKFADTFAHRD
                     RAITDALNIFDETDIISKIEAEEEEEEMQEVVYSLTDARRLNLEYYKNNILHFFIPLC
                     FVATSMVKNPEDSMSLAKIMGDYRFLKGLLWNEFIFDENREDADDVTEALTYLHSKSM
                     ILTSRRDGEAWVEIKSKGEVRLKPFAGLIHNYLESYWIVIRSLVYLKKKPLSQKEWLA
                     KIRSLGDRMFRKGEVLRAEALSQANYQNAILFLESAEMIVSQSREEKGGKREILYSLA
                     NNRAQLEVLRRRLFKFL"
     gene            complement(6426..7028)
                     /locus_tag="PLA58_08005"
     CDS             complement(6426..7028)
                     /locus_tag="PLA58_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62775.1"
                     /translation="MHAREILDSRGNPTVEAEVTTIAGITARAVVPSAASTGEHKMLE
                     LRDDNKKRYNGKGVEKAVRNVLHKIAPEIIGMDCRNQRDIDFAMIELDGAANKGKLGA
                     VATNCGQIKSGSLSRTDRLAKYNQLMRIEEELGDAACYRGMGAFYNLKKKRPAAKRNS
                     AENPKAIFPRWAYGLFSQAHRNLNRRRLSTSSCARLFARL"
     gene            7247..8113
                     /locus_tag="PLA58_08010"
     CDS             7247..8113
                     /locus_tag="PLA58_08010"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOR62776.1"
                     /translation="MTKRTKMIIAFVLILQMVLPYPLYAATVGKFTSVVGNVVLTRDG
                     KDYRPVVNSAVNEKDLIVTGDGASAVMVFTDDSTITLSPKTRMQVREFGMEGKARKGI
                     FSLSIGKLVANVTKFIGGNNKFEVHSPTAVCGVRGTLFGLEVAMVGTVMTTTATCTTG
                     ALSVSALSATGAIVATSGIVAGQTAVITSTGITVGAAGAAGAAGAGAAGSAGTTGAAG
                     AGAAGTTGAAAGTAGAGAAGAAGGAAAAAGVGAGTIATAAAVAAAAVAAAVAATGGTS
                     DTTPTHTTPAHH"
     gene            8208..8957
                     /locus_tag="PLA58_08015"
     CDS             8208..8957
                     /locus_tag="PLA58_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR62777.1"
                     /translation="MANKDSKLFLHLKKWLEKAVLDHRMIEEGDRVLVGVSGGADSYA
                     LLDLLDSPMIFVPRFSFIAVYIDMGFDQEFTAYQSLGKYLQTHQYPYVMEKTDIGTLA
                     HSDFNRKNPCFLCSRLRRKRIFEIADEKGCNKIAFAHHRDDIIETLLINLFYGREIST
                     MMPNQSIFGGKLHIIRPLAYLSEVLLKKYARERQFPHVENACPTSGNSKRSDIKKLLD
                     QLEMDNPEIRHNIYTALSHVKPDYLMIKPKN"
     gene            8972..9448
                     /gene="smpB"
                     /locus_tag="PLA58_08020"
     CDS             8972..9448
                     /gene="smpB"
                     /locus_tag="PLA58_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOR62778.1"
                     /translation="MTKENTAHKLIASNKFARVNYDIDDIYEAGIVLSGTEVKALRAG
                     KANLKDSYAVVKDDEVFLREMHISHYDHGNRFNHEPLRPRKLLLHKREIRKLYGKTRE
                     KGLALVPLRLYFKNGMVKVEIGVGKGKKMHDRREDIKQREERRSIARDFKSKRIKV"
     gene            9502..9990
                     /locus_tag="PLA58_08025"
     CDS             9502..9990
                     /locus_tag="PLA58_08025"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOR62779.1"
                     /translation="MTGKKVSESRVIMGQGMNPENANPAGNVHGGDIMKLIDTAGGVA
                     ATRHARAHVVTASIDRMDFHHPVYVGDFLILKASVNFVGKTSMEVGVRVEAENPITGV
                     KRHTGSAYLTFVALDANKKPMQLPPLILETEEERLRNAEAAERRQMRLAEKKKEKKKH
                     EK"
     gene            10062..10413
                     /gene="ssrA"
                     /locus_tag="PLA58_08030"
     tmRNA           10062..10413
                     /gene="ssrA"
                     /locus_tag="PLA58_08030"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2559 a         2626 c         2920 g         2316 t
ORIGIN      
        1 gatttctccc ttcggtcgaa atgacaaaaa tggccgttat tcaaagttct ccatgtattt
       61 ctccgcaaga gctcaggaat cgtatggacg gcatcattcc cgaaaaagct tacttccgca
      121 ttggagaagt gagcaaaatc cttaatgttg agccgtatgt catccgctac tgggaaacgg
      181 agttcaaaac cgtaaagccg gtgcggacga aaaccgccca gcggctgtac cgcaaaaaag
      241 acgtcgaaga attgctgacc attcgcaatc tgctctaccg agagcgattc acgatcagcg
      301 gggccaaaaa acagttgttg aaaatccgcg gcggccggga agaaccgccc gaagcctgtt
      361 ctcaggataa attagtcctc attaaaagag aattgcagca gattaaaaag atgatgagct
      421 gaaaaagccg gtttttgccg gctttttttc cgcgggaagg caaagattat ttcttgctgg
      481 ctcttttgga ggctttcagc gggttggtct tctgtgcttc cttgatttct atctttacat
      541 gcgatgcggt cggacagttg ccggtcatcc atttgctttt ggggtccgga tagaccttgc
      601 aatacctgcc gatttcgtat tcaaaaattt tattgcaatc cccgcattgt tcgatgaccg
      661 gatggcagct gtttccgaaa aaaccgcatc cttttttcgt catgaagccg cattccactc
      721 cctttttgac cgtatcgcaa agcatcttac acaaccccct tatatagaaa tcccttgaaa
      781 ttttgaacgc tgcggaaagc gacggtccat ccggcggtct ccgcaggggt tccggtaata
      841 aaaaccggcg tgtccttaac agcaaaaaaa ccgactgtca agaaaaatta aagaaaattg
      901 gagctaccgg cacccctgat taaattgaca caacccggca tttgtgttag ttccctcagt
      961 gcataaaaac ggggaaataa tcaagagatg cgcgtttgag ggagtcaatg aaaaggggtt
     1021 ccttactgaa gattttctgg cgaacttttt tcatccatgc gacgctgaac ttccgaagaa
     1081 tgcagaatct cggtttcgct tatgcgatga ttcctctgct ccagaggcgg aaaataccgg
     1141 acaaggaagt tgaaggcgtt ctggtcaggc agctgcagat gttcaacacg aacccctatc
     1201 tggctcaccc gctcgtcggt tcgatcctca aaatggagga agaccggcgc gaaggagagg
     1261 accccgcgcc cattatggcc gtgaagcaga gcctgatggg gccgtatgcc gcgatcggcg
     1321 acactttttt ctggggagcg cttcgaccct gcgcgggaat ctgctccgcc ggtctggttt
     1381 ggctgggctg cgcttttgcg ccgctcgcgt ttcttctgat gtatttaccg gcgcatatcc
     1441 tggtgagatg gcggggattt ttcgaaggct attcgagcgg aaagcatggg attgaatgca
     1501 ttcgcaggat ggatcttccg ggcatttccg gccgtgttcg atggctggcc ctggcggcgc
     1561 tctgcgccgg cagcctctgg tttttgcggg tcggataccg gggcgttgcc gccgaagctc
     1621 ccggatctgc cgcagcggcg gcaatgctgg ccgtcatcct gctttgctgg tggctggtga
     1681 aaagaggagt gtcgcaggta tatctgctgt atggagccgc ggcgctggcg atgatttatt
     1741 caacgagagg tttgtggctt tgctggaaat gaaaaccttt acgttaaaaa ataaactggg
     1801 catgcatgcg cgggcggccg cgctgtttgt gcgaattgcc cagaagtacc gctcggagat
     1861 tgtcattgaa cgcaacggcc agacggtcaa cggaaagagc attctgggaa tcctgaccct
     1921 ggcctgtccg atgggcggac agatcaccgt aagcgccaga ggcagcgacg ccgaccaggc
     1981 gcttgccgaa ctggagacct tgattgaaaa taaatttgga gaagaataaa gtcagcacat
     2041 gatgtcggat caggggaaaa aaacttttgt tctcaaaggc attggcgtct ctccgggcgt
     2101 ggtgatcggc aaggtttacc gctttgatcc gctcgatgcg caaatatcat tttataaact
     2161 tcacagcgcc gacatggttc cgcgcgaaat tgagcgtttc aagaacgcgc tcaaggaatc
     2221 ttcgcggcag ctgctggaaa ttcaggaaaa cctcaggaaa acaaaggtga cggagccgct
     2281 gtacatcatt gatgttcacg ttctgcttct gtcggacaag aaattcatca accgcacgat
     2341 caagtatatt cgccgtctgg gcgtcaacgc ggaatgggcg ctgcgcatga cgctggacca
     2401 ctataaacag gtcttcgaag gcctcgagga tgtttacatc agcggccgca tcagcgacgt
     2461 gcagtacgtg atccagcgga ttcttcgcaa tctttccggc gaaaaacggg aaatggtctg
     2521 ggacgtcggc caggaaggcg tcgtgattgt ctcccacgac ctgtcccccg ccgacacggc
     2581 gcagatgaag atggataaaa tcgtcggttt tgccaccgac agcggcggcc ggacgtcgca
     2641 cacggcgatc gtggcccggt ccatggaact gcccgccgtg gtcggactgg ataatgtcac
     2701 ccgctttgtc cgcaccggcg acgacatcat cgtggacggc acgtcggggc ttgtggtggt
     2761 caatccctat cccgacatgc tcaaacggta tgaggaaaag aaacgccact acgacgaggc
     2821 caaagacgaa tatctgaaat acgcccggca gccggccgtg acactggacc gctatgccgt
     2881 gaaaatcggc tccaatattg aatttgtcga agagatttcc tcggcggtgt cccacggggc
     2941 ggagcacgta ggcctttacc ggacggaatt tctttatatc taccgggatg atttgccgac
     3001 ggaagaggat catttcaaca attaccgcca ggtggttgag gaaaagaatt tttcctggtc
     3061 caccatccgt acgttcgatc tgggcggcga caaatttacc aagtatcaga agcatgccaa
     3121 agaaatgaat ccacagatgg ggctgcgggc gatccgtttc tgcctcaggg aagtggacat
     3181 tttcaagacg cagctgcgcg ccatctggcg ggtgagcgct ctgggcaagg tcaagattct
     3241 gtttcccatg atttcctgca tcgaggaaat ccgggaagcc aaacgcctgc tggaagaggc
     3301 gcgccgggag ctggtgaccc agggagttcc ggtagccgaa cgcatggaga tcggcgccat
     3361 gatcgaagtg ccggccgccg ccgtgattgc cgaccagctg gcgaaagaag tcgattattt
     3421 cagcatcggg acgaacgacc tgatccagta ttcgctggcg atcgaccgat ccaatgaaag
     3481 agtcacctac ctgtacgaac ctctgcatcc ggcggttttg aggctgatca agcgcattgt
     3541 ggatgcggcg cacgaggccg gcattcgcgt ggccatgtgc ggagaaatgg ccggcgatcc
     3601 tctttgctgc ccgattctcc tgggaatgga gctggatgaa ctcagcatga accatcttgc
     3661 gattccgcgc atcaagagaa tccttcagca gtccagcctg gaggagtccc gcaaacttct
     3721 ggcgcaggcc ctgtcctaca aaagcgccgc cgaagttcgg gcctatgtcc agaactacat
     3781 gattcatcac tttcccgacg agtttcagaa gagggatctg taggctccgg atgtgaatcc
     3841 gggtccgtca aacgaaggga ctggagaggt atgagcgaaa aaacgcccac ggaagccggc
     3901 cggcaggaag aagaatatta ctccggctgt atttacgacg agcaggacct gtggcgcttt
     3961 cgtccagtga aagcctatct gtcgcgggtt tccattgccg acgagaacgt ccatgccctg
     4021 aaagaattgg ccggccgcgg cgctgtggtt tatgccatca agcaaagaag caaactcaac
     4081 agcctgatcc tggccgagat caccgcgcgc aatggtctgc ccatgccggt ttacagccac
     4141 ggcgtcaata tgtctttctg gcagccggtg tccaagatga tgaagtttct ctggtcgtct
     4201 ttcctgcgcc gcttcagaca gactgcgctc gcccgccagg ggaaactgga cttccttgcc
     4261 gcaaaagttg ccgcccggaa aagcgtcatt attcatctgg gagagtcgga gttcatccaa
     4321 agccgggccg ccgaggacgc catcgccagc ctgatgaagc tgcaggccgg tctttccttt
     4381 cccgtctata tcgtgccggt gctggtggcc tacggccggc gcagggagaa ggaagacgaa
     4441 agcctgttca acatcctgtt cggtcagacg gaacaaacgg gaacgctccg tcgtctcatc
     4501 actttcatgc gctattccaa gcaggccttt attctccccg ccgagccggt tcatctggcg
     4561 gagtatctgt cggcgaacag tcatttgacc cacaacgaac tcgtcatgga tttgcgcggt
     4621 gaactgattg accggattga agaggaaaag acctccgtgg tcgggccgga actgaaatcg
     4681 cgtgaagccg tgatcggaat ggtcatgcac gacccgaagc tgaaccggtt tctggatgac
     4741 tacgcccaaa aatctaaaaa agaccggacc gctctggccc gggatgcaag gcgctatctg
     4801 catgagattg cgtcggatta ccgggaaacg tttgttgagc tgtgggacaa ggttctgacc
     4861 tggctgtgga acacggtgta tgacggcgta tccatcgact ccgaagggct ggccaacgtc
     4921 cgcaatttgt ccaaaaaaat gccttttgtc atcatttcct gccatcgcag ccacattgat
     4981 tatcttctgc tgtcgtatgt tttttacaaa aataacattc agctgccgtt tattgccgcg
     5041 gggaacaatc tttctttttt cccgatgggg tatattttcc gaaggtccgg agcgtttttt
     5101 ctgcggagga gctttcgcgg gaacgaagtt tatggagagg tcttttccaa atatatggcc
     5161 acactgctca aggaaggcct cccgctcgag ttcttcatcg aaggcggtcg aagccggacc
     5221 ggaaaaatgg tgatgcccaa atacgggctc ttgtccatga ttatccaagc ctatcaggac
     5281 aagtattgcg agaatctggc cgcggtgccc gtgtacatcg gctacgaccg cgtgatcgag
     5341 gaaaagtcct atctgcagga gctctccggc gcgccgaagg tccaggaaaa taccgttgaa
     5401 atcatcaaat ccacgaaaat cctgcgcaaa cggtacggcc gcgtttatgt caacatcggc
     5461 caaccgatca ttttgaagga ctatctggcc gctcaggaaa aatccatgga cgagatgacc
     5521 gtggaagagc gccagagcct ttaccggaaa atcggttacg aggtggtgct ggagatcaat
     5581 aaagtctccg tggtcacgcc gtttgcgctg gtggcctccg gcctcttgag ccatgaccgg
     5641 cgcggcatat cccatgagga gttgcggggc atcttaaacg aattttacga atatctgtcc
     5701 acccggcagg tgaaatttgc agataccttt gctcaccggg accgggcgat tacggacgcc
     5761 ttgaatattt tcgacgagac ggacatcatt tccaagatag aagccgagga agaggaagag
     5821 gaaatgcagg aagtggtcta ttccctcacc gacgccagaa gactgaatct ggagtattat
     5881 aaaaacaaca ttcttcattt tttcattcct ctgtgttttg tggcgacttc catggtcaaa
     5941 aatcctgaag actccatgtc gctggccaaa attatggggg attaccggtt tttgaaggga
     6001 cttttgtgga atgagtttat ttttgacgaa aaccgggagg atgccgatga cgtgacggag
     6061 gctttgacct acctgcactc gaaatccatg attctgacct cgaggcgcga cggcgaagcc
     6121 tgggtggaaa tcaaaagcaa gggggaggtc aggctcaaac cgtttgccgg tctgattcat
     6181 aactacctcg aatcttactg gatcgttatc cgcagcctgg tatatctcaa gaaaaaaccg
     6241 ctttcgcaaa aagaatggct ggccaagatc cgttcactgg gagaccggat gttccgcaag
     6301 ggggaagtcc tgcgcgccga agccctgtcg caagccaact atcagaatgc cattctgttt
     6361 ttggagagtg cggaaatgat cgtttcccag tcccgggaag aaaaaggcgg caaaagggaa
     6421 atcctttaca gcctggcaaa caaccgggcg caactggaag tgctgaggcg acgcctgttt
     6481 aaattcctgt gagcctgtga aaaaaggcca tatgcccatc gcgggaatat ggcctttggg
     6541 ttttccgcgc tatttctttt tgccgccggc cgtttttttt tcaggttgta aaaggcgccc
     6601 atgccgcggt agcaggccgc gtcgccaagt tcttcttcga tgcgcatcaa ctggttgtac
     6661 ttggccagac ggtccgtgcg ggacagtgaa ccgctcttga tctggccgca attggtggcg
     6721 acggcgccga gttttccctt attggcggcg ccgtcgagtt cgatcatggc gaaatcgatg
     6781 tcgcgctgat tgcggcagtc catgccgatg atttccggag caatcttgtg caaaacattc
     6841 ctgacggctt tctccacgcc ttttccgttg tatcttttct tgttgtcgtc gcgcaattcc
     6901 agcattttat gctctccggt ggaagcggcg gaaggaacga cagctctggc ggtgatgcct
     6961 gcgatcgttg tgacttcggc ttcgacggtt ggattgccgc gggaatccag gatttccctg
     7021 gcatgaatac taataatttc aggtatatgg aaacatccct tctgtttttt ccataacaga
     7081 gatgccccgc gacagcaaga tgaagttcct cggatgagaa atgcgaaagg ggagacaaag
     7141 ctctgccatc gggaaaaatg aagatagtcg ttgacaaaaa gggggaaaag taggaaaaaa
     7201 gatcaccttt atcaatcgga ttcacctgct tttcagggag gatgatatga caaagcgcac
     7261 aaaaatgatt atagcgtttg tgttgattct gcaaatggtc cttccgtatc cgttgtatgc
     7321 cgccacggta ggcaagttta cctcggtggt gggaaatgtc gtcctgacaa gggacggcaa
     7381 agattaccgg ccggtcgtca attcagccgt caacgaaaag gatttaatcg ttacgggtga
     7441 tggcgcttcc gcggtcatgg tttttaccga cgacagcacc attaccctgt ccccaaagac
     7501 gcggatgcag gtcagagaat tcggcatgga ggggaaggcc agaaagggaa tcttttcact
     7561 gtccattgga aagctggttg ccaatgtaac caaattcatc ggcggcaaca acaaatttga
     7621 ggttcattcg ccgacagccg tctgcggtgt tcgcggaacc ttgttcgggc tggaagtggc
     7681 catggtcgga accgtcatga cgacgactgc gacctgtacg acgggcgccc tgtccgtgtc
     7741 cgctttgtcg gccaccggcg ccatcgtggc gacgtccggc attgttgcag gtcagacggc
     7801 cgttattaca tccacgggaa ttaccgtcgg cgcggccggt gcagcgggtg ctgcaggagc
     7861 aggtgcggcc ggttcggcag gtacaacggg agcggcaggc gcaggcgcgg ccggcacgac
     7921 cggagcggcg gcaggcacgg caggggcagg cgcggccgga gcggccggtg gagcagcggc
     7981 ggcagccggt gtcggcgccg ggacaattgc taccgcagct gcagtggcag ccgcagcggt
     8041 cgcggcagcg gtagcggcga caggcgggac ttcagataca acccccacgc acacaacgcc
     8101 cgcgcaccat tagaaaaagg ttcatttttc agaaaaacaa caaccccttt cattagcaat
     8161 aaacggttag tgaaagggga ttttttattc ccggaaagta ttgcaggatg gcgaacaaag
     8221 attcaaaatt gtttctgcat ttgaaaaagt ggctggaaaa agcggttctg gaccatcgca
     8281 tgattgaaga aggagaccgt gtgctggtcg gtgtttcagg aggagcggac agctacgcgc
     8341 ttttggatct gctcgactca ccgatgattt ttgttccgcg attttccttt atcgctgtat
     8401 atattgacat gggttttgat caggaattca cggcttatca atcgctggga aaatacctgc
     8461 aaacgcatca atacccgtat gtaatggaaa aaacagacat tgggacgctt gcccattccg
     8521 atttcaaccg gaaaaatccc tgcttcctct gttcccgcct gcgccgcaaa agaatttttg
     8581 aaattgcgga tgaaaaaggc tgcaacaaaa tcgcgtttgc ccaccatcgg gacgacatca
     8641 ttgaaacgct tttgatcaat ctgttttacg gccgggaaat cagcaccatg atgccgaacc
     8701 aatcgatctt tggcggaaag cttcacatta tccgtcccct ggcgtatttg agcgaggtcc
     8761 ttctgaaaaa gtatgcgagg gagcgtcagt ttccccatgt ggaaaacgcc tgcccgacca
     8821 gcggaaattc aaagcgcagc gatatcaaaa aattgctgga tcagcttgaa atggacaatc
     8881 cggagattcg tcataatatt tataccgccc tgagccacgt gaagccggat tatctgatga
     8941 taaaaccgaa aaattaagat agaatcatgc catgacgaaa gaaaacacag ctcataaact
     9001 catcgcgtcg aacaagttcg cgagggtcaa ttacgacatc gacgacattt acgaagcagg
     9061 catcgtactg tccggaacgg aggtcaaggc gctgcgcgcc ggcaaggcca atctcaagga
     9121 cagctacgcc gtcgtcaagg atgacgaggt gtttctgcgc gagatgcaca tcagccatta
     9181 cgaccacggc aaccgcttca accatgagcc gctgcggccg cgcaaactgc tcctgcacaa
     9241 gcgcgaaatc cgcaagctgt acggaaaaac tcgcgaaaag ggactggcgc tggtgccgct
     9301 gagactttat ttcaaaaacg gcatggtgaa ggtggaaatc ggcgtcggca aggggaaaaa
     9361 aatgcacgac cgccgtgagg acatcaaaca aagagaggaa cggcgctcga ttgcgcgcga
     9421 ctttaaatcc aagcggatca aagtgtaaga ccattgcgcc ccctctcttt gcgggagagg
     9481 tcggaggaag ggagccggga catgacaggc aagaaagtca gtgaaagtcg agtgatcatg
     9541 gggcagggga tgaacccgga aaacgccaat cctgccggta atgtccatgg cggagacatc
     9601 atgaagctga ttgatacggc aggcggcgtc gcggccacca gacatgcccg ggcgcacgtt
     9661 gtgacggcat ccattgaccg gatggatttc catcatccgg tttacgtcgg ggatttttta
     9721 atcctcaaag cctccgtcaa ttttgtcggc aagacttcca tggaggtcgg cgtccgcgtg
     9781 gaggcggaaa atcccattac aggggtaaaa cgccataccg gatccgccta tttgaccttt
     9841 gtggcgctgg acgcaaataa aaaaccgatg cagcttcctc ccctgatcct ggaaacggaa
     9901 gaagagcggc ttcgcaatgc ggaagcggca gagaggagac aaatgaggtt ggcggaaaag
     9961 aaaaaagaaa agaaaaaaca cgaaaagtga gatgagcggc tgacgccgct tttctttgac
    10021 aaaccgccgt ttgaggatta tagtatagac aaataaaaaa agggggcgta acggtttcga
    10081 cggggacatt tgaagttgta gaagcgtgcc gaggttccta caggcctcgt taaacaggta
    10141 ggaaacatat aatcgcagac aactacgatt acgctttagc cgcttagacg gctaacgtcg
    10201 aacccggttt tcctgccggt cgggcaagac gtcattcaga caggatagcc aaacttcttt
    10261 cctgggggag ggcggcgaat ccttacagga tagcaaagct ttgatcccgc ctttgggagc
    10321 aagccgcgcg aaggttctaa aacgaaggct acgcacgtag aagctccagc ggaaggtttt
    10381 cggacgcggg ttcaattccc gccgcctcca ccatttagaa a
//
LOCUS       DAOSRM010000080        10104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_6467_length_10104_cov_112.742362, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000080 DAOSRM010000000
VERSION     DAOSRM010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6467_length_10104_cov_112.742362"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLA58_08035"
     CDS             <1..712
                     /locus_tag="PLA58_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOR62780.1"
                     /translation="TLTDLQEEWEINLRILNVRTGQALSAVAMKTRLFKQMELRDSGP
                     WIEDFESADPDPDPAWVLRAETAPVKKNKKANFSVHFDSGQGAEDSSRSLRIDFDFSG
                     DKDFFAFANNYKKRDLTLYNGIEFFVKGTGKFNGQFYILTSHPDDPNRIDQWTAMMVV
                     DKTWKKIRIPFNKMFISRGWIKKGAQKTGAVPGDQVLRLNRVEGIQFGIGSNKNDAVS
                     GSIWIDKIRLYGGSEMPG"
     gene            928..1203
                     /locus_tag="PLA58_08040"
     CDS             928..1203
                     /locus_tag="PLA58_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62781.1"
                     /translation="MKKTLGTIVAAAAVVLLTATFGFAEYAAAGAAQFPYFQLGCLIV
                     GGLIMVSLKRKYNKMYTAEVVGAFALYTLLMALFTNPVIDAVKNIVT"
     gene            complement(1565..2092)
                     /locus_tag="PLA58_08045"
     CDS             complement(1565..2092)
                     /locus_tag="PLA58_08045"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOR62782.1"
                     /translation="MIFACPFAGKPFKKVFLLIPVVLLFLAGNHGFAAEQNNVCTKEI
                     LHTVIHASGLSLGELLKSVADETGKKDMIRTFVKAVRFFPDDSGYIYVYTLDGVVVGH
                     GGMPDLEGKNLIDHRDARGFYDVRMAIATAKKGGGFFEHYWPRPGGTGEFKKLTYVEA
                     IAGTNYFIGAGAYVP"
     gene            complement(2124..2894)
                     /locus_tag="PLA58_08050"
     CDS             complement(2124..2894)
                     /locus_tag="PLA58_08050"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOR62783.1"
                     /translation="MSRFIDLSVAIESGLPSDPPMMIPKIMYVDHALGAESMKAFYPG
                     LTASDLPQGQGWALEVMEVSTHAGTHMDAPWHYHPTQDGGKPALTIDRFPLEWGVGSG
                     VKLDFSAFPDGYNITVEDVQSKLSEIRYALKPGDVFLAQSGAAPYWGTAEYLVKGCGF
                     SRAATLWILDQGVHVVGTDGWSWDRPLPFQAKEFAQTKDASLIWEGHFAGIEKGYFQI
                     EKLTNLDRLPSTGFRFYCFPVKIKGASAGWIRAVAEIL"
     gene            complement(2956..3861)
                     /locus_tag="PLA58_08055"
     CDS             complement(2956..3861)
                     /locus_tag="PLA58_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR62784.1"
                     /translation="MRRQAKGQIHERITAIPNAWYPIYLVRGAEKNLVIDAGVNLLGP
                     RYRNDIREILGDDQPLHDLFLTHSHYDHIGAAGYLKEHFPGLQIGAHERLANLMQKPS
                     VLGLMNGLSANHVELLKYNAAGVDLTLRPFDIDIALKQGDRFDLGGLTCCVHETPGHT
                     KDSLSFYFPEIQALFPGESAGVLQGRTGSEIQVEFLSSYQDYIDSLQWMISLRPEIIC
                     LGHGWVVSGKDAEDFLRRSLAATYHYRELIENYLSSAGGNAQRATEEMAHAEYDVKGG
                     IFQEKISYVTNLNAQVRHIAGLSGL"
     gene            complement(3899..5950)
                     /locus_tag="PLA58_08060"
     CDS             complement(3899..5950)
                     /locus_tag="PLA58_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR62785.1"
                     /translation="METLNRGIACKQDEAAPLVSEEMYRAVVESSPDAMLILDDQFRI
                     IFVNETVCRVSGFSREDYLQRNFMDFLSEENKALVVERYQKRQRGENPPSKYDISYLM
                     KNGESRHAELKTSIFRNSEGKVWTICQMSDVTDLRRAQKALRKSEERFRRITDNMEDL
                     VLQVNLEGTIEFISPSVKKVLDYNIADLIGHSVTSFIHEDDLARAMLVLKKGGKEGEN
                     KTEIRIRHRDGRFFWFEVIGAAIRNEKGKIAGAVVGGRDVTQRKEIEDHLRQSEETFR
                     ALTESTGVAIFVVQGEKFRYINKAFSDLSGYTMEDLRDLRFWDLIAPEMRDFVREQGL
                     ARQRGEEVVSNYEIKYLTKTGEKRIGDFAATFIRYEGRPAMIASISDTTERKKKEEEL
                     RASEERYRTIIENIEDGYFEVNLKGDLIDVSDPCLKITGTDRDGIIGMNFREFSPREN
                     WPKIYQAFYKVFETGEPLRGLAWETVRPDGLRQFIEVSVTLKKNEAGQPVGFRGIVRD
                     VTERKQREETIQWMAYHDILTGLPNRALFYDRAAMVLAQAKRKQWRFGLMMLDLDRFK
                     DINDTYGHDAGDKLLVGVARCLEQAVREQDTVARIGGDEFVIILPEVSGREDVKMIGE
                     RIAEGFRKPISIGQKELTAAVSIGMALYPDDSGDIEGLMRRADEAMYETKVEGGGLHC
                     A"
     gene            complement(6187..7779)
                     /locus_tag="PLA58_08065"
     CDS             complement(6187..7779)
                     /locus_tag="PLA58_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOR62786.1"
                     /translation="MTWKKLNQTWHYVEKWAHETPQGDALVFGDERLNWREFAARMNA
                     VALAYLEMGIKKGDRIALLSMARNEFLTTYMAAGKVGAIWLGLSPKYTLEELRYQLND
                     CRPKVLITLREYMGADLAGTVSALKRECPFLQNVLVIGEPFEGAENFAAFVGKDRRHL
                     ADALAQRAAEVRETDDALLLYTSGSTGKPKGVVHTHRSIVENIKVEVGKFYFDETSRG
                     LLHFPINHVAADVEIGFGAIMSGGCIVCMDKFDPAQTLKVIEQEKITHIGQVPVMFLL
                     EFKQPEFWTCDFSHVRAFVWAGAAAPKVMIDLLGPICQKTGAKLITGYGSTEVCGFIT
                     YTEKDDDVETLLKTAGKIAKPFELKIVDGQRRALPAGEVGEIAVRGPFLMKGYYGRPE
                     ETARVVDREGWYYTSDLAFQDDRGYIHITGRLSEMFKSGGENVYPREVEEVIESHAAV
                     LFAAVIGVPDDIYQEIGWAFVMPTPGQNVTEEDLRELCKNSLANFKVPKKFFIRPMLP
                     LLASGKVNKMALKEEIAEMTKK"
     gene            complement(7798..8673)
                     /locus_tag="PLA58_08070"
     CDS             complement(7798..8673)
                     /locus_tag="PLA58_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOR62787.1"
                     /translation="MKLTSEFVGAPLKDYTCTVEARRTMNYAAAIGDAHPAYFDDEDP
                     GGIIAPPLFPVALTWPIIENITDYLRADAFPREALLTQVHFSEHLRIHQPVIPGMRLT
                     ICGVIAAILPHRAGTYVVIRFDASDDGGRQVFTEHLGAMMRGVECADAGKGGESLPSV
                     PSRTNETILRESSVFIDPLAPFIYDGCTNIYFPIHTSVQFAHQVGLPGIIHQGTATLA
                     VSVHHLLNSEANGDSRRLTTVFCRFSGMVIPGSSIRVCLTGREETDQATDLFFTVFNA
                     EGKKAIRDGYLRLKK"
     gene            complement(9071..9883)
                     /locus_tag="PLA58_08075"
     CDS             complement(9071..9883)
                     /locus_tag="PLA58_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62788.1"
                     /translation="METNAGDINELNRRMELASSLWNLSISRQKNEQREYSHWMGKVK
                     AGVKKVLDLDGAERDRYIEKMIERQVYLFPEEIQPAKPSLFMHMRKEVSYLIPPFDNG
                     RIRFRVEAAIPPDEEDLRLIEKIEALDDHIRRGGDYDDYEELALAVEDESKDRFRNWL
                     IAKGFEDNPEEYVYCPELYLTFLYRYMHEDIVVLKSVSSQYLREFFEDFLLRKMICNK
                     PVEYLYWPPALKLFYQFLNEKGYLSANETDRFLGELEEMGKRFQEIVQERYR"
BASE COUNT         2268 a         2893 c         2518 g         2425 t
ORIGIN      
        1 cacgctcact gatcttcagg aggaatggga aatcaacctg cgaatcctga atgtcaggac
       61 cggccaggct ctgtcagccg tcgccatgaa aacgaggctc ttcaaacaaa tggaactgcg
      121 ggattcaggc ccgtggattg aagattttga aagcgccgat cccgatcccg atcccgcctg
      181 ggtcctccgc gccgaaacgg ccccggtgaa aaaaaataaa aaagccaatt tcagcgtgca
      241 tttcgactcc ggccagggcg ccgaagattc ttcccggtca ttgcgcatcg attttgattt
      301 ctcgggcgac aaggattttt ttgccttcgc caacaactac aaaaaaagag acctgacgct
      361 ctacaacggc atcgaattct tcgtgaaggg aacaggcaaa ttcaacgggc aattttacat
      421 tctgacatcg cacccggatg atcccaacag gatcgaccag tggacggcaa tgatggtcgt
      481 tgacaaaacc tggaaaaaga tccgcatacc cttcaataaa atgttcattt cccgtgggtg
      541 gatcaagaaa ggcgcacaaa agacaggggc cgttcccggc gatcaggtgc tgcgcctcaa
      601 ccgtgtggaa ggcattcagt tcggcatcgg cagcaataaa aatgacgctg tatccggttc
      661 catctggatc gataaaatcc gcctctatgg cggtagtgaa atgcctggct gaaccggctc
      721 cttttcgaca aatcgttaag cttctttaaa atttctttca atcaacaggt cttttctgcc
      781 cgattccgcc agaagcaatt cccagctatt ctcttgtaat atcctgatgt tccaataatc
      841 gttatcctgg gctgcgcttt tggcacgggt ttttcattag gaataaggcg aaagaaacaa
      901 aaaaaccctt gaaggaggaa acagatcatg aagaagacct taggaacaat cgtagcggcg
      961 gcggcagtgg ttcttttgac ggcaaccttt ggatttgcag agtatgcggc ggcaggggcg
     1021 gcccaattcc cgtattttca gctgggctgc ctgattgtgg gcggactgat tatggtcagc
     1081 ctgaagagga agtataacaa gatgtacacg gcggaagtcg tcggcgcctt cgctctttac
     1141 actcttctga tggcgctctt caccaatccg gtgattgacg cagtgaaaaa cattgtgaca
     1201 taatcaaaag cagcagcagg tgaagtaacc gccgtccgga acacaacttt catgatctgt
     1261 catatagaac gccccctcag gttcccacaa ctgagggggt tttttatcgt tgcccaaata
     1321 tccttttcgc ttgcctgaga tcgttttgta taaatctaag gctcgcgaaa ggctttgaca
     1381 aaactcgcct tcggctcaaa cagttgtcat tgctgatctt tcgcttcgcc aagattgtat
     1441 tacaaaacgc tcccaatggc ctctcaaagt gatatctggg caacaagcaa caggtccgtg
     1501 gcgcgggctt tgcttgcgtt tgtaagcgtt tcccgggaat gtcggcattc cgttccctgt
     1561 gtgactatgg aacgtaggcg ccggcgccga taaaatagtt cgtgccggca atggcttcca
     1621 cataagtcag ttttttgaat tcacctgtcc cacccggcct tggccagtaa tgttcaaaaa
     1681 aaccgccgcc ttttttcgcg gtggcgatcg ccatgcgcac gtcatagaag cctctggcat
     1741 cgcggtgatc gatcagattc ttcccctcca gatcaggcat gccgccgtgt ccaaccacca
     1801 cgccatccag tgtatagacg tagatatatc cgctgtcgtc cgggaaaaaa cgaaccgctt
     1861 tgacaaacgt tcggatcatg tctttctttc cggtttcatc ggcgacactt ttgagcaatt
     1921 caccgagcga tagccctgag gcatgaatca ccgtatgcag gatctctttt gtgcagacgt
     1981 tgttctgctc ggccgcaaat ccatgatttc cggctaaaaa caataagacg acaggaatca
     2041 gcagaaaaac ttttttaaac ggttttccgg caaatggaca tgcgaagatc atggctctta
     2101 cctcctcccg acatcaatat cccttataaa atctccgcta ccgccctgat ccagcccgcg
     2161 ctcgccccct tgattttcac cgggaagcag taaaacctga aaccggttga cggaagcctg
     2221 tccagatttg tcagcttttc aatctgaaaa tagccctttt cgatgccggc aaaatgcccc
     2281 tcccaaatga gagacgcgtc tttggtctgt gcaaactctt tggcctgaaa aggcagaggc
     2341 ctgtcccagc tccagccgtc cgtcccgacc acatggacac cctgatccag aatccagagc
     2401 gttgccgccc ggctgaatcc gcagcccttc acaaggtact cggcagtccc ccagtaaggc
     2461 gctgcgccgc tttgcgccag aaagacatct cccggcttaa gtgcatacct tatttcactg
     2521 agtttggatt gaacatcttc aacggtgatg ttatagccgt cgggaaaggc cgaaaagtcg
     2581 agcttcacgc cgctgcccac tccccattcc agaggaaacc ggtcgatggt cagggccggt
     2641 ttgcctccgt cctgtgtggg atgataatgc cagggcgcgt ccatgtgcgt gcccgcgtgc
     2701 gtggaaacct ccatgacctc gagcgcccat ccctgtccct gcggcaggtc ggaagccgtc
     2761 agtccgggat agaaggcctt catgctctcg gcccccaacg catgatccac gtacatgatt
     2821 ttgggaatca tcatcggcgg atcgctgggc aacccgctct caatggccac ggaaaggtca
     2881 atgaaccggc tcatatcagc aactcctttc ttgtaaaagg ataacgcacg attacggcgg
     2941 caggaaaagg aaaagttata gtccgctcag cccggcaatg tgtctcacct gtgcgttcag
     3001 attcgtcacg taggaaattt tctcctgaaa aatgccgcct ttgacgtcgt actcggcatg
     3061 ggccatttcc tccgtggccc tctgtgcgtt gccgcccgct gacgacagat aattctcaat
     3121 caattcgcgg taatggtagg ttgccgccag ggaacgccgt agaaaatcct ccgcatcttt
     3181 gccgctcacc acccagccgt gccccagaca gatgatttcc ggccgaaggg atatcatcca
     3241 ctgaagcgaa tcgatgtagt cctgatagga ggaaaggaat tcaacctgta tttcactgcc
     3301 tgttctcccc tgcagcaccc ccgccgactc gcccggaaac agggcttgaa tttccggaaa
     3361 ataaaaagac agggaatcct ttgtgtggcc gggagtttca tggacgcagc aggtcagccc
     3421 tcccagatca aaccggtcac cctgcttcag cgcaatgtcg atgtcaaagg ggcgcagcgt
     3481 cagatcaacg cctgcggcgt tgtatttcag gagttcaaca tgatttgcgc tgagaccgtt
     3541 catcagacca agcacggacg gcttttgcat caggttcgcc agcctttcat gcgccccgat
     3601 ttgcaggccc ggaaagtgtt ccttcagata gccggcggcg ccgatgtgat cataatggga
     3661 atgcgtcaaa aagagatcgt ggagtggttg gtcatcgccg aggatttccc ggatgtcgtt
     3721 gcggtagcgg ggccccagaa gattcacccc cgcgtctatc accaggttct tttccgcccc
     3781 ccgcaccagg taaatgggat accaggcatt cggaatggcg gtgatccgtt catggatttg
     3841 acctttggcc tgccgtctca taaagaatcg caccgggcgg acggcaatct ctgaaacatc
     3901 atgcgcaatg cagaccgcct ccctcgactt tggtctcgta catcgcctca tccgcgcggc
     3961 gcatcaagcc ttcaatatcg ccggaatcat cgggataaag ggccatgccg atgctgacag
     4021 ccgcagtcaa ttccttttgg ccgatcgaaa tcggttttcg gaagccttcg gcgatcctct
     4081 ctccgatcat cttcacgtcc tcgcggccgg aaacttccgg aagaatgatg acgaactcat
     4141 ccccgccgat gcgggccacc gtatcctgct cccgcaccgc ctgttccagg catcgggcta
     4201 ctccgaccag aagtttgtct ccggcatcgt gtccgtacgt gtcgttgata tccttgaaac
     4261 gatcgagatc cagcatcatg aggccgaacc gccactgttt ccgcttggcc tgggccagca
     4321 ccatggcggc gcggtcgtaa aaaagagcgc ggttgggaag gcccgtcaga atgtcatggt
     4381 aagccatcca ctgaatcgtt tcttctctct gcttgcgctc cgtcacgtcc cggacgatcc
     4441 cccggaaacc gaccggctgt cccgcttcgt tcttttttaa cgtcacggaa acctcgatga
     4501 actgacggag cccgtccggc cgaactgttt cccaggccag acctctcaac ggttcaccgg
     4561 tttcgaaaac cttataaaac gcctggtata ttttaggcca gttctcccgc ggtgaaaatt
     4621 cgcgaaaatt catgccgatg atgccgtccc ggtcggtgcc cgtaatcttc agacagggat
     4681 cgctcacgtc gatcaaatcg cctttgagat tcacctcaaa atagccgtct tcgatattct
     4741 cgataatcgt gcggtagcgc tcttcgctgg cccgcaattc ttcttccttc tttttccgct
     4801 ccgtagtatc ggaaatcgaa gcgatcatgg caggccggcc ctcataacgg ataaaggtcg
     4861 ccgcaaagtc gccgattctc ttttcgccgg tttttgtcag atatttgatt tcataatttg
     4921 atacaacttc ttccccgcgc tggcgggcca gcccctgttc ccggacaaag tctctcatct
     4981 ccggggcgat gagatcccag aagcgaagat cccgcagatc ctccatcgtg taaccggaaa
     5041 gatccgaaaa agctttgttg atgtaacgaa atttctctcc ctgaacgacg aaaatcgcca
     5101 cgcccgtgct ttccgtcagc gcccggaacg tctcctcgct ctggcgcagg tggtcttcga
     5161 tttcttttct ttgcgtaaca tcgcgcccgc ctacgacggc gccggcaatt tttccctttt
     5221 cgttgcggat ggcggcgccg atcacttcaa accagaaaaa acggccgtcc cgatgccgga
     5281 tgcggatttc agtcttgttt tccccttcct tccctccttt tttcaaaacg agcatggccc
     5341 gggcaagatc gtcttcatgg ataaaggaag tgaccgaatg cccgatcaga tcggcaatat
     5401 tgtaatccag aacctttttg accgatgggg aaataaattc gatggtcccc tccagattga
     5461 cctggagaac caggtcctcc atattgtccg taatccggcg gaacctctct tcgcttttcc
     5521 ggagggcttt ctgcgcccgg cgcaaatcgg tgacgtcgct catctggcag atcgtccaca
     5581 cttttccctc gctgttgcgg aaaatggagg ttttcagttc cgcgtggcgg gactctccgt
     5641 tcttcatcag ataggagata tcgtatttgg agggcgggtt ctcaccccgc tgacgttttt
     5701 ggtagcgttc aacaacaaga gccttgtttt cttcggaaag aaaatccatg aaattgcgtt
     5761 gcagataatc ttccctggaa aatccgctga cgcggcagac cgtttcattg acaaagataa
     5821 tgcggaattg atcgtcaaga atcagcatgg cgtccggaga gctttcgacg accgcgcgat
     5881 acatttcttc gctgactaga ggggcggctt catcctgctt gcaggctatt ccacggttca
     5941 atgtttccat tgttcgcctt gccatctacc caacagttga atccaaaaca tacaggcatg
     6001 caaatgccgg aaaagcgatt ttatcttttc aagtcgggaa attctacgtg cgtgctccct
     6061 atataaaata atcatggatg tcaaggcaaa atatgcgcaa acgaccccgc cccgcgaaaa
     6121 cggcccgaaa ctccacatct gacaagcgtc tgtttttatt gaagattcaa aagaaagatc
     6181 tctcggttac tttttcgtca tttctgcgat ttcctctttc agggccatct tgttcacttt
     6241 gccgctcgcc aggaggggca gcatcggccg gataaagaac tttttcggaa ccttgaagtt
     6301 ggccagggag tttttgcaca attcgcgcag atcctcttcc gtgacgtttt gccccggcgt
     6361 cggcatgaca aaggcccatc cgatttcctg atagatgtca tccggaacgc cgattacggc
     6421 ggcaaaaagg acagcggcat gggattcgat cacctcttcc acttcgcgcg gatagacatt
     6481 ttcgccgccg ctcttgaaca tttcggaaag ccttccggta atgtgaatgt atcctctgtc
     6541 atcctggaac gccagatccg acgtgtaata ccagccctcc cggtccacga cccgcgccgt
     6601 ctcttcgggc cggccatagt atcccttcat cagaaaaggc ccgcgtacgg caatttcacc
     6661 gacctcaccc gcgggaagcg cccggcgctg cccgtcaaca atcttcagtt caaaaggctt
     6721 cgcgatttta cccgccgttt tgagcagcgt ttccacgtcg tcatcctttt ccgtataggt
     6781 aatgaagccg cacacttccg tgcttccgta gccggtaatg agcttcgccc ccgtcttttg
     6841 acagatcggt ccgagcagat cgatcatgac tttgggcgcg gccgcgcccg cccacacaaa
     6901 ggcccggaca tggctgaaat cgcatgtcca gaattccggc tgcttgaatt ccagcagaaa
     6961 catcaccggc acctgcccga tgtgggtgat tttctcctgc tcgatgactt tcagcgtctg
     7021 ggccggatca aacttgtcca tgcaaacgat gcagccgccg gacatgatgg ccccgaatcc
     7081 gatttcgaca tccgccgcca catggttgat gggaaagtgg agaagccccc gcgaggtttc
     7141 atcaaaatag aattttccga cttccacctt gatgttttcc acaatgctgc gatgggtatg
     7201 gacaacccct ttgggtttgc cggtggagcc agacgtgtac aagagcaggg catcgtccgt
     7261 ttcccggact tccgccgctc tttgcgcaag cgcgtccgcc aggtgtctcc ggtcttttcc
     7321 aacgaaggcc gcaaaatttt cggcgccttc aaagggttct ccgatcacca ggacattttg
     7381 caggaaagga cattctcttt taagcgcgct gaccgtcccg gcaagatcgg cgcccatgta
     7441 ttcgcgcagg gttatgagca ctttggggcg gcagtcgttg agctgatagc ggagttcctc
     7501 cagggtatat ttcggggaaa gccccagcca gatcgcgccg acttttcccg cagccatgta
     7561 ggtcgtgaga aattcattgc gcgccatgga caaaagcgca atccgatcgc ctttttttat
     7621 ccccatttcc agataggcaa gggcaaccgc attcatccga gccgcgaatt cgcgccagtt
     7681 caggcgctcg tcgccaaaaa ccagcgcatc gccctggggc gtctcatggg cccatttttc
     7741 aacatagtgc cacgtttgat tcagcttttt ccaggtcatg atgcttcctc ccatatttta
     7801 ttttttcagt cgcagatacc catcacgaat cgcttttttc ccttcggcgt taaaaacggt
     7861 gaaaaagaga tccgtcgctt gatctgtttc ctccctgccg gtcagacaga cccggatgga
     7921 tgaaccggga atcaccatcc cggaaaaccg gcaaaagacc gtcgtcaggc gccgggaatc
     7981 gccgttcgct tccgaattca gaagatgatg aacggacaca gccagcgtgg ccgttccctg
     8041 gtgaataatt ccgggcagcc ccacctggtg cgcgaattga acggaggtat gaatggggaa
     8101 ataaatattg gtgcatccgt catagataaa gggagccagc ggatcgatga aaacactgga
     8161 ttcccgcagg atcgtctcgt ttgtccgcga aggaacagaa ggaagacttt cgccgccctt
     8221 tccggcgtcc gcacactcca cgcctctcat catcgcgccg agatgctccg taaatacctg
     8281 ccggcctccg tcatcgctcg catcaaaccg gatcaccacg taggttcccg cccgatgcgg
     8341 caggatggcg gcaatgacgc cgcaaatggt caaccgcatg ccggggatca ccggctggtg
     8401 aatacggagg tgttcgctga agtggacctg agtcaggagc gcctcgcggg gaaacgcatc
     8461 cgcccgcaga taatccgtaa tattctcgat gatcggccag gtcagcgcca cggggaaaag
     8521 gggcggagcg ataatcccac cggggtcttc atcgtcaaag taagccggat gggcgtcgcc
     8581 gatcgccgcg gcataattca tggtccggcg cgcttctact gtgcaggtat agtctttcag
     8641 aggcgcgccg acaaactcgg aggtcaattt catggaagtt ctcctaaccg ggcaaagcca
     8701 aaaccaaaac attttgccgg cgcgtcaata ttttggcttg gatactttca ccctccccac
     8761 cccatcccat caaggaaggg aagaaagctc ctgttcctcc ccccccccct tgtgggttgc
     8821 cgcttacctc tcacgcgcgg tttttccctc tcctcttgtg ggaaagggtc agggcgaggg
     8881 gattcaagtg aatttttcgc gaaaaaacga tgggcgcgcg ggacgcccgc cgtttccggc
     8941 cggcgggaga agaccggcaa gacaaagcgt tctcgacgtg ttcaaatcgc ataattcgta
     9001 tccttcgtca atgaaggatt ctttccgggc gccggacggg tcggctcgac ggggaaaggc
     9061 ggcatgaata tcagcgatac ctttcctgaa cgatttcctg gaaacgtttt cccatctctt
     9121 ccagctctcc gaggaagcgg tctgtctcgt tcgcggacag ataccccttt tcgttcagaa
     9181 actgatagaa gagcttcagg gccggcggcc aatagaggta ttccacgggt ttgttgcaga
     9241 tcatctttct caagagaaaa tcttcaaaga actctctcaa gtattgactg gaaacggatt
     9301 tcagcacgac gatatcttcg tgcatatagc gatagagaaa ggtgaggtac aattccgggc
     9361 aatagacgta ctcctcggga ttatcctcga atcccttggc gatcagccaa ttccgaaagc
     9421 gatctttgct ttcatcttcg acggcgagcg ccagctcctc gtagtcgtcg tagtccccgc
     9481 ccctccggat gtgatcgtca agggcctcga tcttttcgat cagacggagg tcttcttcat
     9541 ccggcggaat ggccgcctcc acccggaagc gaatccggcc gttatcgaag ggcggaatca
     9601 aatagctgac ttccttccgc atgtgcataa acagagaggg ctttgccggc tggatttctt
     9661 cggggaaaag ataaacctgc cgctcaatca tcttctcaat ataacgatcc cgctccgcac
     9721 cgtccaggtc cagaaccttc tttacgccgg ctttcacctt ccccatccaa tgggaatatt
     9781 cccgctgttc gttcttttgc cggctgatgc tcaggttcca cagagacgac gccagctcca
     9841 ttcggcggtt caactcgttg atgtctccgg cgtttgtttc cataaggggt tcggcatatt
     9901 tgatcattgc ctgcgatgaa gaaacggcca tgaatccttg aggcgccgct gcttgcgaaa
     9961 tcggcggccg ttccatgaat gccggcatcg gtctcacctt gcgaacctgc cgtttttgtc
    10021 ttgccttttc tctggccttt ttctgccgct taacaatttc tttcctgttc ttcttcatag
    10081 aacggtttct tttcttgatc aggg
//
LOCUS       DAOSRM010000081        10103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_6470_length_10103_cov_115.418392, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000081 DAOSRM010000000
VERSION     DAOSRM010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6470_length_10103_cov_115.418392"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..1490
                     /locus_tag="PLA58_08080"
     CDS             378..1490
                     /locus_tag="PLA58_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HOR62789.1"
                     /translation="MSDWQIRQAGDAIRLYYFHYRGFKPINIPAEKSEDFTPELLTEA
                     KRLIRLKHYSYSTEKTYLQWIKRFLDYAKQTGRQGPFAELGTDDCKNFLSHLALQERV
                     SASTQNQAFNAILFLFRHVLCKDIGNMAETVRAKRGQKLPVVFSVEEIRKLLACLTGR
                     DLLVAGLLYGSGLRLMELARLRVKDVDMDLNTLTVRSGKGDKDRTTILPATVKELLKN
                     HLINVKKLHESDLANGHGDVHLPDALGKKYPNAGTEWAWQYVFPANNLSVDPRSGKVR
                     RHHISDTAIQDVIKKAIRKADIPKFATVHTLRHSFATHLLMNGVNIREVQELLGHKNV
                     ETTMIYTHVLRDMSHAPKSPLDVLLAGKEERKSKEE"
     gene            complement(1683..3050)
                     /locus_tag="PLA58_08085"
     CDS             complement(1683..3050)
                     /locus_tag="PLA58_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOR62790.1"
                     /translation="MNETILVVDDEVSICQSLKAILEDEGYQVLVAGSGEEALRVVAD
                     EMPQLVLLDIWLPGMDGLDTLKAIRAAHPNMLIIMMSGHGTIETAVKATKLGAFDFIE
                     KPLSLDKVMILVNNALNLVRLEEENILLKQKVSHQYELTGTSPVITELKEMIGIVAPT
                     NAWILIMGENGTGKELVARSIHHLSRRSHKPIVEVNCAAIPEELIESELFGHEKGAFT
                     GATEKKRGKFDLAHEGTIFLDEVADMSLKAQAKILRILQEKKFERVGGNKLIDMDVRV
                     LAATNKDLEKEMEAGRFRQDLYYRLHVIPLRVPPLRDRKEDIIHLTERFLLDFAAKEG
                     QQPKTLSTGALEKLMAHDWPGNVRELKNIIERLIILTPSNEIRAEDIPELSIKTEPDA
                     SFQEAPAAAGDSLRDARLDFERQFILKKLEEYDGNISRTAEAIGLERSNLHKKLKSLK
                     VATKE"
     gene            complement(3081..5303)
                     /locus_tag="PLA58_08090"
     CDS             complement(3081..5303)
                     /locus_tag="PLA58_08090"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR62791.1"
                     /translation="MSLKRDARKSYLDEGELRKRRRERIIMLVVGCLAIVFTILASQL
                     SDRQELPISANILVYGLTSINIILILLLLFLIVRNVFKLFSERRKGLIGSKLRTKLVA
                     AFVGLSLVPTILLFLFAINFLSYSIEFWFNIKIGDALARSLEVAQVYYTQAEDLAKFN
                     ARQISAEITRNRLYEVDRADYLNNFLSQRQKNYKVARVEAYFDFNNKSIVFADGEEAA
                     STAIQLSPKMLEDIYSGKEISTVAATQGGEAIVGVAPVFSYAVATEVIGRVCVSNSVP
                     QGLVDKLRSIANASEQYGQIKLLKNPIKFNYIVTLSIVTLAIIFLATWFGLSLAKSIT
                     NPIQDLVTATNRITRGDLTSRIDIEADDEVGLLVKSFNSMTDDLHKSKMGLIEANTSL
                     EERRKYMAAILRNVSAGIISIDRNGTITTINRAAESMFGIDASPYLFRNYRELLHEDH
                     LALVDSFLQEMKETRMRVLEKHLELTLRDKVLTILLTLTTIEDEEGKDSGIVVVFEDL
                     TKLQMAERAAAWREVARRMAHEIKNPLTPVQLSAQRLQRKYGDKLGEEGSVFKECTQT
                     IIDQVDVLKNLVNEFSRYARMPVTTLAPNDLNAVVSDAVALFVDAHKDIHFEFRPAEG
                     LPRFHLDAEQINRVMVNLLDNAVASLNKQTGHVGVLIRYDEAHRKAVVAVADDGVGVP
                     ADYRRKVFDPYFSTKKSGTGLGLAIVSSIISDHKGTVTVSDNHPTGTIVSFQLPVNEV
                     "
     gene            complement(5344..5928)
                     /locus_tag="PLA58_08095"
     CDS             complement(5344..5928)
                     /locus_tag="PLA58_08095"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOR62792.1"
                     /translation="MKKHKNDFWAAVFVGLCMMTMTVAAGEARAQMDPRMTDILVTGG
                     TGEMLLYARLVDGFKKEMEEAILAGVPAVFTIELDVYQERPYFWDRHILHREIRRTIK
                     YDNLKKSFLIATNGLGQPAILTNFESAQKAMAELNGIPVVAFSNLSKGERYYVQVRVK
                     IDRVRLPFKMEYVLFFVSLWDFETPLYKIRFAYE"
     gene            complement(6024..6953)
                     /gene="lpxC"
                     /locus_tag="PLA58_08100"
     CDS             complement(6024..6953)
                     /gene="lpxC"
                     /locus_tag="PLA58_08100"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOR62793.1"
                     /translation="MHLQRTLKKEIFCSSIGLHTGRKVNMQINPASADTGIVFVRKDL
                     PNARPIPADFKSVCDTTLATTIGDNGNTVSTIEHLMSAFCGMGLDNAVVEIDSFEVPI
                     MDGSARPFVNMLKKVGTRPQGKFKKMLVIKKPVSVKEGEGSAMLLPSNEFKITYDIDF
                     KNKIIGRQSYSFLFSDKNYEDNICCARTFGFLKEVEFLQARGLGLGGSLENAIILDEE
                     KIINKEGLRMPDEFVKHKILDAVGDLFLLGMPIIGHFVAHRSGHRLNNLLLRELMREQ
                     DSWEIISEDHPQQSSQFTACNLPSFTILDAVQI"
     gene            complement(7181..8149)
                     /gene="ruvB"
                     /locus_tag="PLA58_08105"
     CDS             complement(7181..8149)
                     /gene="ruvB"
                     /locus_tag="PLA58_08105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOR62794.1"
                     /translation="MENSLRPPKLADYVGQDKVKSNLAIFIEAARKRRESLDHVLLYG
                     PPGLGKTTLAYIIARELGVDIKVTSGPVIERPGDLAAILTNLHEHEVLFIDEIHRLSH
                     VVEEILYPAMEDFHIDILIGQGPSARSMKLDIPKFTLVGATTRAGSLTSPLRDRFGMH
                     FRLEFYSPQELAVILARSASILNVKLTVDGARELAHRARGTPRIANRLLKRVRDYAEV
                     KGDGTIDGDLACRALDAFEVDQKGFDQMDRKLLLTLIEKFNGGPVGVESLAAAIGEER
                     VTIEDVYEPYLIQEGYLQRTARGRVACSKAYEHFGLKASGAQKELF"
     gene            complement(8237..8845)
                     /gene="ruvA"
                     /locus_tag="PLA58_08110"
     CDS             complement(8237..8845)
                     /gene="ruvA"
                     /locus_tag="PLA58_08110"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOR62795.1"
                     /translation="MIAMLSGKIAYKGISHVVVDAQGVGYRVFIPLTTFYALPEADQA
                     ITLHIHTSVKEDAIHLFGFYTREERELFQLMISVSGIGPKVALNILSGISSAELLEAI
                     SAGNLTRLMAIPGIGRKMAERLILELKEKVMKKMAADAIPAADAKQKQAEMMREDALS
                     ALMNLGYKAGAARDALEKAARDLKEEPAMDQLLKKALKILAG"
     gene            complement(8846..9325)
                     /gene="ruvC"
                     /locus_tag="PLA58_08115"
     CDS             complement(8846..9325)
                     /gene="ruvC"
                     /locus_tag="PLA58_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOR62796.1"
                     /translation="MRILGIDPGSHVTGYGVIDKSGNYLRHVAHGEIKARRNSLLSEV
                     LMDTFRELGAVIADNNPQAVSLENIFYGKNVRSLIRQAQVRGVVICACADRGLPVFEY
                     SPLEVKKAVVGYGRAEKRQVQIMVKAILKLDGLPPADAADALAAAICQANFMKEQPI"
     gene            complement(9360..>10103)
                     /locus_tag="PLA58_08120"
     CDS             complement(9360..>10103)
                     /locus_tag="PLA58_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOR62797.1"
                     /translation="GHSKWSTIKRKKGAIDAKRGKIFTKIIKEITLAARLGGGDIEGN
                     ARLRQAVMAAKEENMPKDNIERAIKKGTGGGDGANAYEEFTYEGYGPGGAAVIVEIMT
                     DNKNRTVAEIRHIFSKHGGNLGENGCVAWMFAKKGSIVIDRKAIGEDDLMELALDAGA
                     EDVKTEDSEYEVITEPSAFEAVKKALDAKGIKHLEARISMIPSNTVKLEAGKAESMLK
                     LMEKLEDNDDVQNVYSNFDIDEDVMEKLS"
BASE COUNT         2231 a         2835 c         2575 g         2462 t
ORIGIN      
        1 ccaacaatta atatgccaac tttgtttttt ccgatatgat gagtagtttt actctttttt
       61 gaaaagaatg caaagaaaaa agtgtttata aatcaattaa aatgaacttt ttacacttgg
      121 caatttgtag taatataccg tttgagccaa gttggtatat tatggaaaat ttgtccgaca
      181 gcaggaaaaa cgacgttctt ccagattttc aagcctttct tttggataag aaactggcgc
      241 aggaaaagaa tgtctttttt tatgctttgt gggccagcaa atttttaaat tatgcaaaca
      301 gaaagcaaat accgcccgat caatatcagg aaaacgccgt catagaattt atcgaatctt
      361 tgaaatcaaa ttccgccatg tccgactggc aaatccggca ggcaggcgac gccattcgtt
      421 tatattattt tcattaccgc ggcttcaaac cgataaatat accggcggag aaatccgaag
      481 actttacacc ggaactgctc acagaggcaa aacgtctgat ccggcttaaa cactactctt
      541 acagcacgga aaaaacatat ctacaatgga tcaaacggtt cctggattat gccaaacaaa
      601 ccggccgaca gggacccttt gctgaacttg gaacggatga ttgcaaaaat ttcctcagtc
      661 atctcgcgct tcaggaaaga gtgtccgctt caacgcagaa ccaggcattc aatgccattc
      721 tatttctttt ccgtcatgtg ctttgcaagg atataggtaa tatggcggaa accgttcgcg
      781 caaaacgcgg gcagaaattg ccggttgtct tctcggttga agagatcagg aaattgttgg
      841 cctgtctgac cggcagggat ttactggttg ccggtttact ctatggttcg ggactgcgat
      901 tgatggagct tgcgcgcctg agagtgaagg atgtcgatat ggatttgaat acgttgaccg
      961 ttcgtagcgg aaagggggat aaagacagga cgactattct gccggcaacc gtgaaagagc
     1021 tattaaaaaa tcatttaata aacgtgaaaa agctgcatga aagtgatctt gccaacggcc
     1081 atggcgacgt ccatcttccc gacgcccttg gcaaaaaata tcccaacgct ggcacggagt
     1141 gggcatggca gtacgttttc ccggcgaaca acctttctgt tgatccgcgt tccgggaaag
     1201 tccggcgcca tcatatcagc gatacagcaa tacaggatgt tataaaaaaa gcgatccgca
     1261 aggccgatat tcccaaattc gccactgttc atacacttcg tcacagtttc gcgacgcatt
     1321 tgctgatgaa tggtgtcaat atccgcgagg tgcaagagct gctcggccac aagaatgtgg
     1381 aaacgacgat gatttacacg cacgtgctgc gggatatgtc ccatgcgccg aaaagcccac
     1441 tggatgtgct attggctggc aaagaagaga gaaaatcaaa agaggaatga agaaagagat
     1501 aaagcttttc aataaagagg atcgccatag aaatgttcgt tattaatttc ggactgaaag
     1561 gttggatgcc cgtcaaaatt gcacgatgct ccctttcagg aagggcgcat caaccggaag
     1621 atccctattt tttattccag atgaagtgcg gggcacatcc atgtaacctt cagcctttat
     1681 ccctattcct tcgtcgccac cttcaagctc ttgagttttt tgtgcagatt gctgcgctcc
     1741 aggccgatgg cttcggcggt cctggagata ttgccgtcgt attcttccag ctttttcaaa
     1801 ataaactgcc gttcaaaatc cagtcttgcg tccctgagcg aatctccggc cgccgcgggc
     1861 gcttcctgaa aagacgcatc cggctcggtc ttgatgctca gctcgggaat gtcctccgcc
     1921 cgtatttcat tggacggcgt cagaataatc agcctttcaa tgatgttttt cagctcacgg
     1981 acgttgcccg gccagtcatg cgccattaac ttttccaggg cgccggtgct cagggtcttc
     2041 ggctgctgcc cctccttggc ggcaaagtcc agcagaaacc gctccgtcag gtgaatgatg
     2101 tcctctttcc ggtcgcgcag aggcgggaca cgaagaggaa tgacatgcag gcggtagtaa
     2161 agatcctgcc ggaaccggcc cgcctccatt tccttctcca ggtccttgtt ggtcgcggcg
     2221 agaaccctga cgtccatgtc gatcagcttg ttgccgccga ccctctcaaa tttcttttcc
     2281 tggagaatcc gcagaatctt ggcctgggcc ttgaggctca tgtccgccac ttcatccaga
     2341 aagatcgttc cctcgtgcgc caggtcgaac ttgccgcgct tcttttcggt cgcccccgtg
     2401 aaggcgccct tttcatgccc gaacagctcg ctttcgatca gctcttcggg aatggccgcg
     2461 cagttgactt ccacgatggg cttgtgggag cgtcggctca ggtggtggat ggagcgcgcc
     2521 accagctcct ttcccgtgcc gttctcgccc atgatcagaa tccacgcgtt ggtcggcgcg
     2581 acaatgccga tcatttcctt gagttcggtg atgaccgggc tggttccggt caattcgtac
     2641 tggtgcgata ccttctgttt gagcagaata ttttcttctt ccaggcgaac cagattcagc
     2701 gcgttgttca ccaggatcat cactttgtcc agcgacagcg gtttttcaat aaagtcaaaa
     2761 gcgcccagct tggtggcttt cacggccgtc tcgatggtgc cgtggccgga catcatgatg
     2821 atcagcatgt tcggatgggc ggcgcggatg gccttgaggg tatccagacc gtccatgccg
     2881 ggcagccaga tgtccagcag aacgagctgg ggcatttcat cggccacaac ccgaagcgcc
     2941 tcttcgccgc tgccggccac gagcacctga tagccttcgt cttccagaat ggccttcagc
     3001 gattgacaga tgctgacttc gtcatctacg accagaatgg tttcgttcat gatgttcacc
     3061 catgccgcgt cttctataaa ttaaacttcg ttcacgggca actgaaaaga tacaatcgtt
     3121 cccgtggggt ggttgtcgct gacggtaacg gttcccttgt ggtccgagat aatggagctg
     3181 acgatggcca gccccaatcc cgttcccgac ttcttggttg aaaaatacgg gtcaaacact
     3241 tttcgcctgt agtccgccgg aaccccgacc ccgtcgtcgg ctacggcgac caccgctttt
     3301 ctgtgcgcct catcataccg gatcagaacg ccgacatgcc ccgtctgctt gttcagagac
     3361 gccacggcgt tgtccagcag attcaccatg acccggttga tctgttcggc atccagatgg
     3421 aacctcggca gcccctccgc cggacggaat tcgaagtgta tgtctttgtg cgcgtccaca
     3481 aaaagcgcca cggcatcgga caccaccgcg ttaagatcat tgggggccag cgtcgtcacc
     3541 ggcatgcgcg catagcggga gaattcattc accagattct tcagcacgtc cacctgatcg
     3601 atgatggtct gtgtgcattc cttaaaaacc gaaccttcct cccccagctt atccccgtat
     3661 ttccgctgca gcctctgggc ggagagctgc acgggcgtga gcggattctt gatttcatgc
     3721 gccatgcgcc gggccacttc ccgccaggcc gcggcgcgct ccgccatctg cagcttcgtc
     3781 aaatcttcaa acacaacgac aatgccggag tccttgcctt cttcgtcctc aatggtcgtc
     3841 agggtcagca gaatcgtcaa caccttgtcc cgcagcgtca gttccagatg cttttccaga
     3901 acccgcatgc gggtttcctt catttcctgc aaaaaggaat cgaccagggc gagatgatcc
     3961 tcgtgcaaaa gctcccggta attccggaac agatacggag aagcgtcaat gccgaacatg
     4021 gattccgcgg cgcggttgat ggtcgtgatg gttccgttcc ggtcgatgga gatgatgccc
     4081 gccgagacat tgcgcagaat ggcggccatg tactttcgcc gctcctccag cgacgtattg
     4141 gcctcgatca ggcccatctt gcttttgtgc aaatcatccg tcatggaatt gaaggacttg
     4201 accaacaggc cgacctcgtc gtcggcttca atgtcaatgc ggctggtcag gtccccccgc
     4261 gtgatgcggt tggtcgccgt tacaaggtcc tggatggggt tggtaatgct cttggccaac
     4321 gacaggccga accacgtcgc gagaaaaata atcgccagcg tcacgatgga cagggtcacg
     4381 atgtaattga acttgatcgg attcttcagg agcttgatct gtccgtactg ctccgacgcg
     4441 ttggcgatgc tcctgagctt gtccaccagc ccctggggaa cgctgttgct gacgcatacc
     4501 cgcccgatga cttccgtcgc cacggcataa gaaaaaaccg gcgcgacgcc gacaatggcc
     4561 tccccgccct gggtggcggc caccgtggag atttcctttc cggagtaaat gtcttccagc
     4621 atcttcggcg acaactggat ggcggtggac gccgcttctt cgccgtcggc aaagacgatg
     4681 cttttgttgt tgaaatcaaa atacgcttcc acgcgcgcca ccttgtagtt cttttggcgc
     4741 tgggaaagaa aattgttcag ataatcggcc cgatccacct cgtagaggcg gttgcgggtg
     4801 atttccgcgc tgatttgacg ggcgttgaat ttggccaggt cctcggcctg cgtgtaatac
     4861 acctgtgcga cttccagcga ccgcgcaagg gcgtcgccga ttttgatgtt gaaccagaac
     4921 tcgatcgaat aggaaagaaa gttgatggca aacaaaaaaa gcaaaatggt gggaaccagg
     4981 gaaaggccca cgaacgccgc aaccagcttg gtgcgcagct tcgagccgat caatcccttg
     5041 cgacgctcgg aaaacagctt gaaaacattg cggacaatca gaaaaagcag aagcagaatc
     5101 aggatgatgt tgatgctggt caggccgtac accagaatgt tggccgaaat gggcagttct
     5161 tgccggtcgg acaactgact ggccagaatc gtaaatacaa tcgccagaca gccgaccacc
     5221 agcatgatga tgcgctcacg ccggcgtttt ctcagctccc cttcgtcaag atagcttttt
     5281 ctggcgtctc tcttcaaact catgcgctcg cggcatcacc ctttcccgcc cccgcgccgg
     5341 cgatcactcg taggcaaacc ggatcttgta caggggggtt tcaaagtccc acagagaaac
     5401 aaagaacaac acatattcca tcttgaacgg caggcgcacg cggtcgattt tgacccggac
     5461 ctgaacatag taccgttcgc ctttggacag attggaaaag gccaccacgg gaattccgtt
     5521 caactccgcc atggcttttt gcgcgctttc aaaattcgtc agaatcgccg gctgccccag
     5581 gccgtttgtg gcaatcagaa aactcttttt caaattgtcg tatttaatgg tgcggcggat
     5641 ttccctgtgg aggatatgcc gatcccaaaa gtagggcctt tcctgataga cgtccagttc
     5701 tatcgtaaaa acggcgggga cccccgccag gatggcctcc tccatttcct tcttgaaacc
     5761 gtccaccagc ctcgcgtaca ggagcatttc ccccgtgccg ccggtcacca gaatatccgt
     5821 catccgggga tccatctggg ctctggcctc cccggcggca accgtcatgg tcatcatgca
     5881 taacccaaca aagacggccg cccagaaatc atttttatgc tttttcatac ctgcccgttt
     5941 tctttccgaa agaatacggg tatcctaggt aacctatcga taaaaatcaa ggttaatttg
     6001 gtaaggtcaa aacggggaca aaatcatatt tgaacggcgt ccagaatcgt gaaggacgga
     6061 aggttgcagg ctgtgaattg agacgattgc tgcggatgat cttcgctgat aatttcccaa
     6121 ctgtcctgct cgcgcatgag ttcccgaagc agaaggttgt tgaggcggtg gccggaccgg
     6181 tgcgccacaa aatggccgat gatcggcatg cccaacaaaa acaaatcgcc cacggcatcc
     6241 aggattttgt gcttgacgaa ttcatcgggc atgcggaggc cttccttgtt gatgattttt
     6301 tcctcatcga ggataatcgc attctccagc gatccgccca gccccaggcc tctggcctgc
     6361 aaaaattcga cttctttcag aaaaccgaag gttctcgcgc agcagatgtt gtcctcgtag
     6421 tttttatcgg aaaacaggaa gctgtaagac tgccggccga tgattttatt tttgaaatcg
     6481 atgtcatagg taattttaaa ttcattcgag gggagcagca tggcgcttcc ctcaccttct
     6541 tttacggaaa ccggtttttt gatgaccagc atttttttga attttccctg cggccgcgtg
     6601 ccgacttttt tgagcatatt gacaaacggg cgggcgctgc cgtccatgat gggcacttca
     6661 aaggaatcga tttccacaac ggcattgtcc agccccatgc cgcaaaacgc cgacatcaga
     6721 tgctcgatgg tcgaaaccgt attgccgtta tcgccaatgg ttgtggccag ggtcgtgtcg
     6781 caaaccgact tgaagtccgc cggaatcggc ctggcattcg gcagatcctt ccggacgaaa
     6841 acaatgccgg tgtccgcgga agcgggattg atctgcatat tgaccttgcg gcccgtgtgc
     6901 agaccgatgc tggagcagaa tatttctttt tttaaagtac gctgtaaatg catgttactc
     6961 tcttttttaa gttgaatttt acgtttagca ataaacatac cagcgcaaag agcatggggc
     7021 ctgtattgca tattattgta tttattgact ttatacagga gtcactccgg agggaatgat
     7081 cgactggttc gctattttgt ggttaaaaca acacaccggc ccgtatcgag attgtttatt
     7141 gtaaggctat gatttgttta taaaatactt tatttacccg ctaaaacagc tccttttggg
     7201 cccccgacgc tttcaggccg aagtgctcgt atgctttgga acaggcgaca cgcccccgcg
     7261 ctgtccgctg cagataccct tcctgaatca aatagggttc gtaaacatct tcgatcgtga
     7321 ctcgctcctc gccgatggcc gccgcaaggc tttccacgcc gaccgggccg ccgttgaatt
     7381 tttcaatcag cgtcaaaagc agtttgcggt ccatctgatc aaaacccttt tgatcgacct
     7441 cgaacgcgtc cagcgcccgg caggccagat cgccgtcgat ggttccgtcg cctttcactt
     7501 ccgcgtaatc gcgcacgcgc ttgagcaggc ggttggcgat ccgcggcgtg ccgcgcgccc
     7561 gatgggccag ttcccgcgcc ccgtccaccg tcagcttcac attcagaatg gaggcggagc
     7621 gcgccagaat cacggccagc tcctgcggcg aatagaattc cagtcggaaa tgcatgccga
     7681 agcggtcgcg cagcggcgag gtgagtgatc cggctcgcgt ggtcgcgccc accagcgtga
     7741 atttcggaat gtcgagtttc atcgaccggg ccgacggccc ctgaccgatc agaatatcga
     7801 tgtggaaatc ctccatggcc gggtagagaa tttcttcgac gacgtgcgaa agccggtgaa
     7861 tctcgtcgat aaacagcacc tcgtgctcgt gcagattggt cagaatcgcc gccagatcgc
     7921 cgggacgctc gatcaccgga ccggacgtga ccttgatgtc cacgcccagc tcccgggcaa
     7981 tgatataggc cagcgtggtt tttcccaggc ccggcggtcc gtatagaaga acatgatcca
     8041 acgactcgcg ccgtttgcgg gccgcctcga tgaaaatcgc cagattgctc tttaccttgt
     8101 cctggcccac ataatcagcc agcttcggcg gacgcagcga attttccagc gctccttcgt
     8161 cttcggctca ggccggatcg atcagcggat ttttcaaatg agcatccatg cgtttctacc
     8221 ttcaaaccgg cgggcctcaa cccgccagaa ttttcagcgc tttcttcagg agctggtcca
     8281 tcgccggttc ttccttcaga tcgcgcgcgg ctttctccag agcatcccgg gccgctcctg
     8341 ccttgtatcc cagattcatc agggcggaaa gcgcgtcttc gcgcatcatc tccgcctgtt
     8401 tttgtttcgc gtccgcggcc gggattgcat ccgcggccat ttttttcatg accttttctt
     8461 tgagctccag aatcagccgc tcggccattt tccggccgat gccgggaatg gccatgagcc
     8521 tggtcaggtt gcccgcggaa atcgcctcga gcagctccgc ggaggaaatt cccgaaagaa
     8581 tattcagcgc gactttcggc ccgatgccgc tgacggaaat catgagctga aaaagctccc
     8641 gttcttcacg cgtgtaaaaa ccgaacagat gaatcgcgtc ctctttgacc gaggtgtgga
     8701 tatgcagggt gatggcctga tccgcttccg gaagcgcata aaaagttgtg agcggaataa
     8761 aaacacgata gccgacgccc tgcgcgtcca ccacaacgtg ggaaattccc ttataggcga
     8821 ttttaccgga aagcatggca atcattcaga tcggctgctc cttcatgaag ttggcctggc
     8881 agatcgccgc ggccagcgcg tccgccgcgt ccgccggcgg caaaccgtca agtttcaaaa
     8941 tcgccttgac catgatctgc acctgccgtt tttcggcccg gccgtagccg accaccgcct
     9001 ttttcacttc cagcggcgaa tattcaaaaa ccggaagccc ccggtccgcg caggcacaga
     9061 tcacgactcc gcgcacctga gcctgccgaa tcaggctgcg gacatttttc ccgtaaaaga
     9121 tgttttccag gctgaccgcc tgcggattat tatcagcgat cacggcgccg agctcgcgga
     9181 aggtatccat cagcacttcg gaaagaaggg aattcctgcg ggccttgatt tcaccgtggg
     9241 ccacatgacg caggtagttg ccgcttttat caatcacgcc gtagccggtc acatgagaac
     9301 cgggatcgat gcctaagatt cgcaaattac cctctttcgc ttccggccgt ttgggcggac
     9361 taactcaact tctccatgac gtcttcgtca atgtcgaaat tggaatagac gttctggacg
     9421 tcgtcgttgt cctcgagctt ttccatcagc ttcagcatgg actccgcctt gcccgcttcc
     9481 agcttgacgg tgttggaggg aatcatgctg atgcgcgcct ccagatgctt gatgcccttc
     9541 gcgtcgaggg ctttcttcac tgcctcaaag gctgacggct ccgtgatcac ttcatattcc
     9601 gaatcttccg ttttgacgtc ttccgctccc gcgtcgaggg ccagttccat cagatcgtct
     9661 tcaccgatcg cttttctgtc gatgacgatg ctgcctttct tggcaaacat ccaggccacg
     9721 cagccgttct cgcccagatt tcctccgtgc ttggaaaaga tatgccggat ctccgccacc
     9781 gtccggtttt tattgtccgt catgatttcc acgatgaccg ccgcgccgcc cggtccgtag
     9841 ccttcgtagg taaattcttc atacgcgttt gcgccgtcac cgccgcccgt gcctttcttg
     9901 atcgcccttt cgatattgtc cttgggcata ttttcttcct tggccgccat gaccgcctgc
     9961 ctcaagcggg cattgccttc tatgtcgccg ccgcccaaac gcgccgccag cgtgatttcc
    10021 ttgatgattt tggtaaagat cttgccgcgt ttggcgtcaa tcgccccttt ttttcttttg
    10081 atggtgctcc atttggaatg gcc
//
LOCUS       DAOSRM010000082         9789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_6697_length_9789_cov_118.976885, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000082 DAOSRM010000000
VERSION     DAOSRM010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9789
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6697_length_9789_cov_118.976885"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1245)
                     /gene="gcvPB"
                     /locus_tag="PLA58_08125"
     CDS             complement(<1..>1245)
                     /gene="gcvPB"
                     /locus_tag="PLA58_08125"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOR62798.1"
                     /translation="IGAKYLRGDLDLPEVAQIDLVRHYTNLSRRNFGVDLGFYPLGSC
                     TMKYNPKINENIAALPGFTDLHPLAPEAFAQGSLELMYDMQTLLSALFGMTDFTLQPA
                     AGAHGELTGVMMIKKYFEKRGEKRRQILIPDAAHGTNPASGALCGFETVTLRSNAEGG
                     VDLAHLESLMTEDVAALMLTNPNTLGLFERNIEKVAAIVHGRGGLLYGDGANANAYLG
                     KTRPGDLGFDVIQLNLHKTFSTPHGGGGPGSGPVGVGKKLVDFLPVPRVVKNGAAYSL
                     SEDFPDTIGRVRAFYGNFNVIVKACAYLRSLGAEGLRRATEMAVLNANYIQKRLKEHF
                     DLPYDRICMHECVFSGSRRAAETGVHTTDVAKRLIDFGYHPPTIYFPLIVPEAIMIEP
                     TETESKETLDAFCDAMIAIAKEA"
     assembly_gap    1246..1345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1346..2421)
                     /gene="gcvPA"
                     /locus_tag="PLA58_08130"
     CDS             complement(<1346..2421)
                     /gene="gcvPA"
                     /locus_tag="PLA58_08130"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOR62799.1"
                     /translation="MDYCPHTPEDIAQMQAAIGIGGIEELFADIPQKFRLREIPGMPA
                     ALSEQEIWERMKAQSEKNRAPRATLTGAGAYHHFIPAVVNSIVGRAEFYTAYTPYQAE
                     ISQGILQAIYEYQTMIARLTGTEIANASMYDGASAMAEAAVLCIRMSNRSKIAVARSV
                     HPQYRRVLQTYLWANGCTLTEIPWLPAGQIDPPALSAALDKETAAVIVQSPNFFGCIE
                     DIGPLADAAHQQGAMLIAGFTDATSLGLLKPAGESGADFVVGEGQSFGNPLNYGGPYL
                     GIFAAREKYLRRIPGRLAGATVDQNGRRGFVLTLQTREQHIRREKATSNICSNEALCA
                     LAAGVYLAALGKNLGKLAALNIHKT"
     gene            complement(2426..2824)
                     /gene="gcvH"
                     /locus_tag="PLA58_08135"
     CDS             complement(2426..2824)
                     /gene="gcvH"
                     /locus_tag="PLA58_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOR62800.1"
                     /translation="MSKPNPTDRLYSKDHEWILDRGDGTALVGITDYAQEMLTDIVFV
                     ELPPIGKKVAAGEPLAVVESVKSVSDVYSPAGGEVIAVNTALENAPELVNRDAFGEGW
                     IARLKLADARELKTLLDAAAYDALILEEKH"
     gene            complement(2845..3936)
                     /gene="gcvT"
                     /locus_tag="PLA58_08140"
     CDS             complement(2845..3936)
                     /gene="gcvT"
                     /locus_tag="PLA58_08140"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOR62801.1"
                     /translation="MKKTPLYDRHAARNAKMIDFGGWAMPVQYTSVIEEHRTTRARAS
                     VFDICHMGEIEVKGPQALDLLQFALTRDLAGQKTGQVKLSALLNDRGGIIDDLTVYKM
                     DRDFYMLVTNAATRESDFQRVRSILEEKKFDASLTDVSDRTGKLDLQGPLSEAILQTL
                     TPDDLKTLRFYWFIPSQVAGIPAVISRSGYTGEDGFEIYAAADKIGAIWDSLMDAGVK
                     QGMKPAGLGARDTLRLESGMMLNGQDITGETCPLEVPYAWLVDWNKDFAGKPALAAIR
                     ERGIRKKLAGFEMTGRGIARHGYPVSKDGREIGYVTSGTFSPTLNKAIGLAFIDAENA
                     EPDSEIAIGIRDAASGAKIVQLPFYKRQK"
     gene            complement(3965..4522)
                     /locus_tag="PLA58_08145"
     CDS             complement(3965..4522)
                     /locus_tag="PLA58_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOR62802.1"
                     /translation="MAPKRVLTLWNDNGVNEADTSVLRKKSQDMKTPLDREEKAEIKA
                     LIDSFTCRDDASGLAGPQIGVNKRIIIFRNKTGNDRKKKMTPDDYDVLVNPRITQARG
                     EKVKAAEGCLSCPDIQIDVERFPEIKVRALNEKGEKISKRYLDWTARVVQHEIDHLDG
                     KLIVDYEGTLYYPKSKQALIDKLFK"
     gene            4665..5939
                     /locus_tag="PLA58_08150"
     CDS             4665..5939
                     /locus_tag="PLA58_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HOR62803.1"
                     /translation="MISSIVAHTSKKQFARALNFQDGPTPELPACADDQPRLLYIHIP
                     FCEELCPYCSFHRIRLNEPLARSYHAALRREMSLYRLRGYRFSGLYVGGGTPTVLMDE
                     LAETLQIARELFPVESVSVETNPNHLTDANIAALAQAGVNRLSVGVQTFDDGLLRKIA
                     RYDKYGSGEDIASRLRAAQGAFDTLNADMIFNFPEQTAPMLEADLAILLKLGMQQITF
                     YPLMVSTLTRRLMQKTLGDVNFRREKSFFNRIRRRLEPQYDSSSAWCFSRKSPASSLI
                     DEYVVDFDEYAGLGSGAIGYLQGVCYANTFDIERYIRDLTQDRLPLQASRQFDLPDRM
                     RYDFLMKLFSTRMNVEELEKKYGGRFFKTLWKEMAAFILAGSFRFFSPHLYLTPRGRY
                     LWVVMMREFFIAVNNFRDHCRREAGLEERAVE"
     gene            6029..7447
                     /gene="selA"
                     /locus_tag="PLA58_08155"
     CDS             6029..7447
                     /gene="selA"
                     /locus_tag="PLA58_08155"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOR62804.1"
                     /translation="MDDKRKVLLKGLPKIDDVIGLLEKNGIYAKASKDIVREACRQVV
                     QNLRDQILSAADKTLPSVSPDAASAARSVEEIVAGLHRFKLKRLVNATGVILHTNLGR
                     APLCSEAVDRIVEVGRGYSNLEFDLIRGERGLRYDHVGGLICALTGAEDALIVNNNAA
                     AVLLTLNTLAEHKEAIVSRGELIEIGGEFRIPDVMTKSNSILREVGTTNRTRLADYEK
                     AIGPNTGVILKVHTSNYRIVGFTEEAELVPLVALGKHHGIPVFDDLGSGCLVDLAAFG
                     LQHEPTVREVLGSGVDVVTFSGDKLLGGPQAGIIVGKKDIVARIKKNPLNRALRIDKF
                     TLAALEATLMRYLAPGDAPRSLRSLKALTEPQGDVRKRARKLMGKLKRAGLSGLTYEL
                     KDSMAAAGGGSLPTEDIPSAVVAVKSAKMPASRMEARLRRAAVPVIVRVDENEILIDL
                     RTVAEDEFGFVVDALRNLAASP"
     gene            7523..8548
                     /locus_tag="PLA58_08160"
     CDS             7523..8548
                     /locus_tag="PLA58_08160"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOR62805.1"
                     /translation="MSIENQESRNVSFTVGPGQAGLRLDVFLSQADPAFSRSQIKYAV
                     EEGDVLVNGREPKASQHLKAGDRVDLHWEPVMEAAAVPQDIPLNIVYEDSSVIVVNKP
                     AGMVVHPAPGNPDQTLVNALLFHCKDLSGIGGVLRPGIVHRLDKETSGLIVAAKTDEA
                     HRKLSAQFEKHHVHKKYIALVWGDVRGESGEIVLPVGRHPKDRKKMSTKSRHGRDALT
                     LWKVRERYTVATLLEVEIKTGRTHQIRVHLSERGYPVIGDAVYGTSSKGQTVKNAELK
                     AGIKALARQALHAAELSFLHPQSGERMVFTAPLPQDLESLLHLLRAVSPGSAKQTGLP
                     AWKDQLR"
     gene            8693..9547
                     /gene="pgeF"
                     /locus_tag="PLA58_08165"
     CDS             8693..9547
                     /gene="pgeF"
                     /locus_tag="PLA58_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOR62806.1"
                     /translation="MFSWLNKNSIPYLQSSGLAACDFLAHAFCTREGGVSEDDYQSLN
                     MSFREGDEEFHVLQNWNKLAGAFGIPMENFLVLNQVHRDGILVIPPRGEYFTSREGLD
                     YDAIVTNRTNLAICIKTADCAPIFVVDPENKVIAAVHAGWRGTAASISAKVVRLMQNQ
                     YGSRPSALLAAIGPSIGRCCYEVDEPAAAAFCSHKNAKAFLLPGKRKDRWMLDLAQAN
                     RGQLAESGIPDAGIDASGLCTVCRQDAFFSHRGSGGVTGRQINFLMLKGDPFCKALFV
                     NEDFRQVH"
     gene            9578..>9789
                     /locus_tag="PLA58_08170"
     CDS             9578..>9789
                     /locus_tag="PLA58_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR62807.1"
                     /translation="MNLKPLFEPKTMAVIGISAGNERHPANVIYAKNNHRYPVRVYAV
                     NPRGGTLHNETIYPRVSEIPARIDLAV"
BASE COUNT         1911 a         2761 c         2858 g         2159 t
ORIGIN      
        1 gcctcttttg caatggcgat catggcgtcg cagaaggcgt cgagcgtttc tttgctttct
       61 gtttccgtcg gttcgatcat gatggcttcc ggcacgatga gcgggaaata gatggtgggc
      121 gggtggtagc cgaaatcgat cagccgcttg gcaacatcgg tcgtgtgcac gccggtttcg
      181 gctgcccggc gactgccgga aaagacgcac tcgtgcatgc agatccggtc ataggggagg
      241 tcgaaatgct ctttgagtct tttctggata tagttcgcgt tgagcacggc catctccgtc
      301 gcgcgccgga ggccttccgc gcccagcgag cggaggtagg cgcaggcttt gacgatgacg
      361 ttgaagtttc cgtaaaaggc gcggacgcgg ccgatggtat cgggaaaatc ttccgacaga
      421 ctgtatgcag cgccgttttt caccacgcgg ggcacgggca ggaaatcaac cagctttttc
      481 cccacgccca ccgggccgct gcccgggccg ccgccgccgt gcggcgtgga gaaggttttg
      541 tgcaggttca actggatgac gtcaaagccc agatcgccgg gcctcgtttt gcccagatac
      601 gcgttggcgt tcgccccgtc gccgtaaagc aggccgcccc tgccgtggac gatggctgcc
      661 actttttcga tgttgcgttc aaacaggccc agcgtgttgg ggttggtgag catcagcgcg
      721 gcgacgtctt cggtcatcag ggattccaga tgcgcaaggt caacgccgcc ttccgcgttg
      781 gagcgcagcg tgaccgtctc gaacccgcac agcgcgccgg aggcgggatt ggttccatgc
      841 gccgcgtcgg gaatcaggat ctgacggcgc ttttctcccc gtttctcgaa atattttttg
      901 atcatcatca cgccggtcag ttcgccgtgc gcgccggccg ccggctgcag cgtgaaatcc
      961 gtcatgccga aaagcgcgct caacagtgtt tgcatgtcgt acatcagctc caggcttccc
     1021 tgggcgaatg cctccggcgc gagcgggtgc aggtcggtaa agccgggcag ggcagcgata
     1081 ttttcgttga ttttgggatt gtatttcatc gtgcaggaac ccagcggata aaaaccgaga
     1141 tcgacgccga agttccgccg cgataaattg gtgtagtgcc ggaccagatc gatttgggcc
     1201 acttccggca ggtccaggtc gccgcgaagg tattttgcgc cgatgnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnngtttt gtggatgttg agcgcggcca gtttgcccag
     1381 gtttttgccc agcgcggcca gataaacccc tgccgccagc gcgcagagcg cttcgttgga
     1441 gcagatgttg gaggtggcct tttcccggcg gatgtgctgt tcacgggttt gcagcgtcag
     1501 gacgaatccg cgccggccgt tttgatccac tgttgcgccg gcgagccggc cgggaatccg
     1561 ccgcagatat ttttcgcgcg ccgcgaaaat ccccagatac gggccgccgt agttaagcgg
     1621 gttgccgaag ctctggccct cgcccacgac gaaatccgct ccgctttcgc ccgcgggctt
     1681 gagaaggccc aggcttgtgg cgtcggtaaa tccggcaatg agcatcgcgc cctgttggtg
     1741 ggccgcgtcg gccaacggcc cgatatcttc gatgcagccg aagaaattcg ggctttgcac
     1801 gatgaccgcc gccgtttcct tgtccagcgc ggctgaaagc gccggcggat cgatttggcc
     1861 cgcgggcagc cagggaattt cggtcagcgt gcagccgttg gcccacaggt aggtttgcaa
     1921 aacccgccgg tactggggat gaacggaacg cgccacggcg attttcgatc ggttggacat
     1981 ccggatgcac agcacggccg cttccgccat ggccgaagcg ccgtcataca tggaggcatt
     2041 ggcgatttcc gtcccggtga gacgggcgat catggtctgg tattcgtaaa tagcctgcag
     2101 gatgccctgg ctgatttccg cctggtaagg cgtgtaagcc gtataaaatt cggcgcgtcc
     2161 gacgatggaa ttcaccaccg ccggaatgaa gtgatgataa gccccggcgc cggtaagcgt
     2221 tgcgcgcggc gcccggtttt tttcgctctg cgccttcatc cgctcccaga tttcctgctc
     2281 ggagagcgcg gccggcatgc cgggaatttc ccgcaggcga aatttttgcg gaatatcggc
     2341 aaacagctct tcgatgccgc cgatgccgat ggccgcctgc atctgcgcga tgtcttcggg
     2401 agtgtggggg cagtaatcca tggatttagt gcttctcctc caaaatcaaa gcgtcgtagg
     2461 cggccgcgtc cagaagggtt ttcagctccc gcgcgtcggc gagtttcagc cgggcgatcc
     2521 atccctcgcc gaaagcatcc cggttgacaa gctccggcgc gttttcgagg gctgtgttga
     2581 cggcgatcac ctcgccgcct gccggcgagt aaacgtcgga tacggatttg acggattcca
     2641 ccacggccag cggttctccg gcggcgactt ttttgccgat cggaggaagt tccacaaaca
     2701 cgatgtccgt cagcatttcc tgggcgtaat cggtgatgcc gaccagggcc gttccgtcgc
     2761 cccggtccag gatccattca tgatctttcg aatacaatct gtcggtggga tttggtttgg
     2821 acatgccgga tctccttaaa ggatttattt ttgtcttttg tagaagggaa gctgaacgat
     2881 ttttgctcca ctggcggcgt cgcggatgcc gatggcgatt tccgagtccg gctcggcgtt
     2941 ttcggcgtca ataaacgcga ggccgattgc cttgttgagc gtcggggaaa acgtcccgga
     3001 ggtgacataa ccgatttctc tgccgtcttt cgaaaccggg tagccgtgcc gtgcgatgcc
     3061 gcgcccggtc atctcgaagc cggccagttt tttgcggatg cctctttccc ggatggcggc
     3121 cagcgccggc tttccggcaa aatccttgtt ccagtccacc agccaggcgt agggaacctc
     3181 cagcgggcag gtctccccgg taatatcctg gccgttgagc atcatgccgg attccaggcg
     3241 cagcgtgtcg cgcgctccca gtccggccgg tttcatgccc tgtttgacgc ctgcgtccat
     3301 caggctgtcc cagatggccc cgattttgtc ggcggcggcg taaatttcga agccgtcctc
     3361 gccggtgtag ccgctgcggg aaatgacggc cggaataccc gccacctgcg aggggataaa
     3421 ccaataaaaa cgaagggttt tcaaatcgtc cggcgtcagg gtctgcaaaa ttgcttccga
     3481 gagcggcccc tgcaaatcca gtttgccggt cctgtcgctt acatcggtca gcgaggcgtc
     3541 aaactttttt tcttccagga tggatcggac ccgttgaaaa tcggattcac gcgtagcggc
     3601 gttggtcacc agcatataaa aatcccggtc catcttgtaa acagtcaggt cgtcaatgat
     3661 gccgccccgg tcattgagca gggcggagag tttgacctga ccggtttttt gccccgccag
     3721 gtcgcgcgtc agggcaaact gaagcagatc cagcgcctgc ggccctttca cttcgatttc
     3781 tcccatgtgg cagatgtcaa acacggaagc cctggcgcgg gttgtgcggt gttcttcgat
     3841 gacgcttgtg tattgcaccg gcatggccca gccgccgaaa tcgatcattt tggcgtttcg
     3901 ggcggcatga cggtcgtaca aaggcgtttt tttcatgaaa agcgctcctt cgttgagtct
     3961 gctgctattt gaaaagcttg tcgatcaggg cctgtttgct tttcgggtag tagagggttc
     4021 cctcataatc cactatgagt ttgccgtcca gatgatcgat ttcatgctga accacgcggg
     4081 cggtccagtc cagatagcgc ttgctgattt tttcgccttt ttcgtttaag gcgcgaacct
     4141 tgatttcggg aaaccgttcc acgtcgattt gaatgtccgg gcaggaaagg cacccttcgg
     4201 ccgcctttac tttttctccg cgcgcctgcg taatgcgcgg attgaccaga acatcgtaat
     4261 cgtccggcgt catttttttc ttccggtcgt tgcccgtttt gttgcggaaa atgatgattc
     4321 ttttattgac gccgatctgc ggaccggcca gccccgaggc gtcgtcccgg caggtaaagg
     4381 aatcgattaa cgccttgatt tcggcttttt cttccctgtc cagcggtgtt ttcatgtcct
     4441 gtgacttttt ccgcaggacg gaggtgtccg cctcgttgac accgttgtca ttccaaaggg
     4501 ttaagactct tttgggggcc attttttttg caccggttcg ctgaattttc atgacctata
     4561 acaccgatca atcgttattt caagtaaacg cagcttgcaa atttgctcca tcttttgtta
     4621 aacagacaaa ccattaattt taatgcagcg aaagaatttg cgttgtgatt tcttccatcg
     4681 tcgcccatac atcgaaaaaa cagtttgccc gcgccctgaa ttttcaggac ggtccgacac
     4741 ccgagttgcc tgcctgcgcc gacgaccagc cgcggctgct ttatatccat attccttttt
     4801 gcgaagaact ttgtccctac tgctcctttc accgcattcg gctcaatgaa cctcttgccc
     4861 ggagctatca tgcggccctg cgccgggaga tgtcccttta ccgccttcgg ggctaccggt
     4921 tttccggcct ttacgtaggc ggcggaacgc ccacggttct gatggacgaa ctggcggaaa
     4981 cgctgcagat tgcccgggag ttgtttcccg tcgaatccgt ttcggtggaa accaatccga
     5041 accacctgac ggacgccaac attgccgccc tcgcgcaggc aggggtcaac cggctgtccg
     5101 tgggcgtgca gaccttcgat gacggcctgc tcaggaaaat tgcgcggtat gacaaatacg
     5161 gatcgggaga agacatcgcc tcgcgtctgc gcgccgcgca gggggcgttt gacacgctca
     5221 acgccgacat gatcttcaat tttcccgaac agaccgcccc catgctggag gccgatctgg
     5281 cgattttgct gaagctggga atgcagcaga tcacttttta ccctttaatg gtttccacgc
     5341 ttacccggcg tctgatgcaa aaaacactgg gagacgtcaa tttccggcgt gaaaaatcgt
     5401 ttttcaatcg gatccgtcgg cgtctggaac cgcagtatga ttcttcatcg gcctggtgtt
     5461 tttcgcgcaa gagtcccgct tcatcgctca tcgatgagta cgtcgtcgat tttgacgaat
     5521 atgcgggatt ggggagcggc gccatcgggt acctgcaggg cgtgtgctac gctaacacgt
     5581 ttgatattga gcgctacatc agggatctga cgcaggaccg cctgccgctt caggcctcgc
     5641 gccaattcga tctgccagac cggatgcgct acgatttcct gatgaagctc ttcagcaccc
     5701 ggatgaatgt cgaggagctg gagaaaaaat acggcgggcg gttttttaaa accttgtgga
     5761 aggaaatggc cgcttttatt ctagccggat cgtttcgctt cttttcgccc catttgtatc
     5821 tgacgccccg cggacgctat ttatgggtgg tcatgatgcg ggaattcttc atcgcggtga
     5881 acaatttccg cgaccactgc cgccgggagg cgggcctgga ggaaagagcg gtggagtagc
     5941 ccgcgcagct tcgcgaggct cctccggcgg cgggagaatc aggacaggga agccgtttcc
     6001 cgcaaaagcg gcatcaggag aggtttttat ggacgacaaa agaaaagtct tgctcaaggg
     6061 ccttccgaaa attgacgacg tgatcggact tctggaaaaa aacggaattt atgcaaaggc
     6121 gtcgaaggac attgttcggg aagcctgccg tcaggtcgtt cagaacctgc gcgatcaaat
     6181 tctgtccgca gcggacaaaa ctctgccgtc agtctccccc gacgcggcgt cggccgcccg
     6241 cagcgtggag gagatcgtcg ccggtttgca tcgcttcaaa ctgaagcgcc tggtcaacgc
     6301 caccggtgtc atcctgcata ccaatctggg gcgcgcgcct ctttgttccg aagccgtgga
     6361 ccgcattgtg gaagtcggac gcggctactc caacctggaa tttgatttga ttcgcggcga
     6421 acgcggcctg cgctacgatc atgtcggggg cctgatctgc gcgctcaccg gcgcagagga
     6481 cgctctgatc gtcaacaaca acgcggccgc ggttttgctc acgctcaata cgctggccga
     6541 gcataaggaa gcgattgtct cgcgcgggga gcttatcgaa atcggcgggg agttccgcat
     6601 cccggacgtc atgaccaaaa gcaattccat attgcgcgag gtgggaacca ccaaccgcac
     6661 gcggcttgcc gattatgaaa aagccatcgg accgaacacc ggcgtgattc tgaaagtgca
     6721 caccagcaat taccggattg tcggatttac cgaggaggcg gaacttgtgc ccctggtggc
     6781 gctgggcaag caccatggga ttcccgtttt tgacgacctc ggcagcggtt gcctggtgga
     6841 ccttgcggca ttcgggctgc agcacgaacc gaccgtgcgc gaagttctgg gctcgggtgt
     6901 cgatgtggtg accttcagcg gcgacaagct cctgggcgga ccgcaggcgg gcatcattgt
     6961 cggcaaaaaa gacatcgtgg cgcgcatcaa aaaaaatccg ctgaaccgtg cgctgcgcat
     7021 cgacaaattc acgctggcgg ccctcgaggc gaccctgatg cgctatctcg cgcccgggga
     7081 cgctccccgg agcctgcgct ccctcaaagc gctgaccgag ccgcagggcg atgtccgaaa
     7141 gcgcgcccgc aaactgatgg gaaaactcaa gcgcgccggt ctttccggcc taacgtatga
     7201 gctgaaagat tccatggcgg cggcgggagg cggatcgctc ccaaccgagg atattccctc
     7261 ggccgtcgtt gcggtgaaat ccgccaaaat gcccgccagc cggatggaag ccaggctgcg
     7321 ccgggcggcg gtgcccgtta ttgtccgcgt agatgaaaac gaaatcctga tcgatctgcg
     7381 cacggtggcc gaagacgagt ttggctttgt ggtggatgcc ctgcgaaacc ttgcggcctc
     7441 cccatgacgc cataggtatg gaggaggcag ggggatttcg gatttttgta tctttcattt
     7501 gtttatttat cggagcagtg ttttgtccat cgaaaatcag gaatcccgaa atgtatcttt
     7561 taccgtcggc cccgggcagg ccgggcttcg tctggacgtg tttctctccc aggccgatcc
     7621 ggctttttca cgctcgcaga tcaagtatgc cgttgaggaa ggcgacgtgc tggtcaatgg
     7681 aagggagccg aaggcgagtc agcatctcaa ggcaggcgac cgggtggatc tgcattggga
     7741 gccggtgatg gaggccgccg ccgtaccgca ggacattccc ctgaacattg tgtacgaaga
     7801 cagctccgtc atcgtcgtca acaaaccggc gggcatggtg gtgcaccccg ctccgggcaa
     7861 tcccgatcaa accctcgtca atgcgcttct ttttcactgc aaagaccttt ccggcatcgg
     7921 cggcgtcctg cggccgggga ttgtccaccg cctggacaag gaaacctccg gtctgatcgt
     7981 cgccgccaaa accgacgaag cgcatcgtaa actgtccgcg cagtttgaaa agcatcacgt
     8041 gcacaagaaa tatatcgcgc tggtctgggg agacgtccgg ggcgaaagcg gcgagattgt
     8101 tctgccggtg ggacgccatc cgaaagaccg caaaaaaatg tccacaaaaa gccggcatgg
     8161 ccgggacgcc ctcacgctgt ggaaagtgcg ggaacgctac accgtcgcga ccctgctgga
     8221 ggttgaaatc aagaccggac ggacgcatca gattcgtgtg catctttccg aacgaggcta
     8281 tcccgtgatc ggcgatgcgg tttacggaac gtcgtccaaa gggcaaaccg tcaaaaacgc
     8341 ggaactgaaa gcgggcatca aagccctggc ccggcaggcg ctgcatgccg cggagctttc
     8401 cttcctgcac ccgcaaagcg gcgaacgaat ggtttttacc gcgccgctgc cgcaggatct
     8461 ggaaagcctg cttcatcttt tgcgcgccgt ttcaccgggc tcggcaaagc aaaccggttt
     8521 gccggcctgg aaggatcaat tacgataaac ggcaaaaaaa ggtatccgac tattgcgggg
     8581 aagcgcaacg ggtgttaaat agtcatgggg ctttttcagt ggtttcggct gtatcctatt
     8641 ttccccaacc cctctttgaa gaaagagggc acatgacgca gggcaaaagt ccatgttttc
     8701 atggttgaac aaaaacagca tcccctatct gcaatcgtct ggtctcgcgg cctgtgattt
     8761 tctggcgcac gctttctgca cccgtgaggg aggcgtttcg gaggatgact accaaagcct
     8821 caatatgagc ttccgggaag gcgacgaaga atttcatgtg ctgcaaaact ggaataaact
     8881 ggccggcgcg ttcggcatcc cgatggaaaa tttcctggtg ctcaaccagg tgcaccggga
     8941 cggcattttg gttatcccgc cgcggggtga gtattttact tcgcgcgaag ggctggatta
     9001 tgacgccatc gtgaccaacc gcaccaatct ggccatctgc atcaaaacgg cggattgcgc
     9061 gccgatattt gtggtggatc ccgaaaacaa agtgatcgcg gccgtgcatg cgggatggcg
     9121 cgggaccgcc gccagcattt cggccaaagt cgtccgcctg atgcaaaatc aatacggctc
     9181 ccgtccttcc gctctgctgg cggccatcgg gccgtccatc ggccgctgct gctatgaagt
     9241 ggacgagccc gccgccgcgg cgttttgttc ccacaaaaat gcaaaagcct ttttattacc
     9301 gggcaagcga aaggaccgct ggatgctgga tctggcgcag gccaatcgcg gacagcttgc
     9361 cgagagcggc attcccgacg ccggcattga cgcctccggt ttgtgcaccg tctgccgtca
     9421 ggacgctttt ttttcacacc gcggttcggg cggcgtcaca ggaagacaaa tcaattttct
     9481 catgctgaag ggagatccgt tctgcaaggc tctgttcgtc aacgaggatt ttcgtcaggt
     9541 ccattgatga aaaccgtaac ctgaaaagga aggtctcatg aatctgaagc ctttgtttga
     9601 gcccaaaacc atggcggtca tcggcatttc cgcaggcaac gaacgccatc ccgccaacgt
     9661 gatctacgcc aaaaacaacc atcgctatcc cgtcagggtt tacgcggtca atcccagagg
     9721 cggcacgctg cacaacgaaa cgatttatcc ccgcgtgtcg gagattccgg ccaggatcga
     9781 tctggccgt
//
LOCUS       DAOSRM010000083         9766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_6719_length_9766_cov_120.455566, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000083 DAOSRM010000000
VERSION     DAOSRM010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6719_length_9766_cov_120.455566"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PLA58_08175"
     CDS             complement(<1..781)
                     /locus_tag="PLA58_08175"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOR62808.1"
                     /translation="MKEVVVVGGVRTAIGDYMGSLSGLTPVQLGVTVLKAAIAKAGID
                     RNLVQEVVAGQCNQAGAPGNSARHIALGAELPVESFAFTVHQQCPSSMRASELLSQEI
                     MLGKIDIGAVVGIESMSNAPYLLFGARKGYRLNDGEKIQDSLMIGGLVDALLGYHMGV
                     TADNIADLYKISRQEQDEWALMSHQRACAAIQNGWFKDEIVPVEVKTKKGTVLFDTDE
                     HPRADASMESLSKLKPAFKKDGTVTAGNASGLNDAGSCMIMM"
     gene            1913..2788
                     /locus_tag="PLA58_08180"
     CDS             1913..2788
                     /locus_tag="PLA58_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR62809.1"
                     /translation="MATYIENVLVYTFVMGSIYLLLALGFSLICGVLRIFHLGYAYIF
                     PLTIYGTWMFMSALGWPLIPSILGMIVVQFIVAIAIYKGMIKRYMTTEDVLLTALLLI
                     ALIVEQAANYFYPIQAGVYLETTLAEGTYQFGDAVVPKQLIIGSLIALAMTGAFVFFF
                     VKTKTGMAIRALSQDIYSSKIVGINVETLYMFTMMLVLLPVIIGTLIVAPVWALDPGM
                     GWMYMVTAILVAVLGGLGNIKGTILASFLIGFAHALVCFVLGEPRFMNLSALILVMAI
                     LIIRPQGLARSESLW"
     gene            2782..3822
                     /locus_tag="PLA58_08185"
     CDS             2782..3822
                     /locus_tag="PLA58_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR62810.1"
                     /translation="MVETELKRDRIVVRLGTKKWEWTLEPRYWRNTLIWAVVLFIFPT
                     VSYFISPGLINTMISANIYAAIAMPLALMTIGTGRMNFGPQFYIGVGGYTAALLSIAY
                     GWGPLATFPFAVLMSMISALIFSPLVIMARGLYYVLLTLLLPLVFLEVTFIYPDIFKG
                     DTGLFGIVPLLDTGNAKNNFLLIGYISTAIMFAYLWIINKVLKSRYGLLMAAINDDED
                     VAHSIGININKIKIIAFVSASTMIGIIGWFYAHYFSSFAGITYLPLTFMLKVLLVLMI
                     GGRAQVFGCVVGGYFVAFLEMLMIRYMGPIQPVLFPIILLALLFILPEGLFGIYRKRR
                     YREYLPTIHVRR"
     gene            3829..4575
                     /locus_tag="PLA58_08190"
     CDS             3829..4575
                     /locus_tag="PLA58_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR62811.1"
                     /translation="MTPLLKVSNLVKRFGGIVAVNNVSFEIYEGETLGFIGPNGAGKS
                     TVVNCLTGYLPATSGKIEMNGVDISRMKPHERAHMGLARTYQIPRPFPELTALTSVLA
                     SSVSGKKRVNQSFEDAMYDATHYLEFVGLFSKRNILARDLTFYELRMLELARALATTP
                     QLLFIDEVMAGLNPGEAKNAIRMIRKAKEQFGITVFWIEHVMAVLMEAADRIVAINYG
                     AKLAEGTPDQVVNNDEVIEAYLGRGWREYA"
     gene            4568..5272
                     /locus_tag="PLA58_08195"
     CDS             4568..5272
                     /locus_tag="PLA58_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR62812.1"
                     /translation="MLEVSDLSASYGVIPALHGVSFNVRQSEIVTILGSNGTGKSTIL
                     NVIQGMMTGTSGSVIFRDEAIQGLPSHKIVDLGIVQVPEGAKSFPFMTVKENLTLGAY
                     RKTSWPKRKQTIQYVYSLFPILKERSNMSARLLSGGEQQMLKIGRGLMANPHLLMVDE
                     PSIGLAPLIVDEVYETLGKLRKTGLTILLTEQNALRALQLADRGYIIQEGRIVLEGRS
                     AELMKNNLVKKAYLGR"
     gene            5275..6630
                     /locus_tag="PLA58_08200"
     CDS             5275..6630
                     /locus_tag="PLA58_08200"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOR62813.1"
                     /translation="MEDYFSYTAKLFNPDEADRKPEALKGIRVLDFSHVIFGPIAARL
                     LADYGAEVIKLELPFYGDLWRPATYWGKYWKHSNPIWHNITKNKYFLSVNLQLKESKE
                     LIYRMVKDADVVIENFAPGTADAWDIGYEKISKINSSIIFLSCSTYGQFGPMRYFPGW
                     DLLAQGASGVLTLNGYPDTDIFYKLPDYVGDFMPGNFAALSVLMALYHRKKTGKGQYI
                     DMAQTEGLMRTLFHYTYYSVTQKAIGRTGNVDPTMVPAAIFKTADDKFLALACATSEQ
                     FENFTRAIGRPELSSDPRFSDTLERLKPENAKALHAIAVDWIRSQSCADVIRLADDKN
                     IPAAEVADDYMIANEEWRRKRGSVVLFKDDVYGNLVIAGPSAMLSGTPSRTKWLARPL
                     GYHNRFVLKKFLGLSEAEIEKLEKKKVIGTFDDRPGLKPPVYYDLSKDPIYNYGKEVG
                     K"
     gene            6627..7949
                     /locus_tag="PLA58_08205"
     CDS             6627..7949
                     /locus_tag="PLA58_08205"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOR62814.1"
                     /translation="MSPREEVYTRHGGKGGDRGKILAEMMNAEGKPEVLDDILVLDVS
                     SANFAGIVAASFFAEFGAEVIKIEPPEGDPARLITPFGVTVQGVGVPFMFEGRNKRYM
                     TLDVKNNPEDRKVFARLSKKAAVVIESFAPGEMDGWGIGYRQLVAENPGLIYIALSAY
                     GQYTDKARESAKMPDTDITSQAGSGLPAQMGAPPNEPEPYTWPLRGGMWAGWYISGLS
                     AALGGAVALLHRQNTGQGQMVDVAGMDAYSSMVGMPATIGFTWEKARPRIGVLDFILY
                     PYGHWKCKDGYVAIAAPRDHDFRALLKVLGLWEQEDDWRYSSDRIPDILEQAIHLHKI
                     IEEKTLQFTADELVKMALNYSKKSARSKWRGGGVPIVMKLMTPEEAMENKQWHIRKAF
                     QEVEDKKLGKYTISSNFVKMSESPPRVKWVGSEIGGDNEYIRKKYLND"
     gene            8022..9383
                     /locus_tag="PLA58_08210"
     CDS             8022..9383
                     /locus_tag="PLA58_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR62815.1"
                     /translation="MMKRASRVKMVAGAALLLLVAIAISVPSVAVAQPKGEPFVIGYI
                     GMVGSPGTRPCMDIQKMAVEEINAAGGILGRPVKYVVMDNKGDTSLSVEAFRKLVIED
                     KAKFISVEGRTEVCLAVQEASGMLFKEYPHILIFNGPMGAELTARIIDEAPKYNHCFR
                     DWDPEPAHYAQTKYFFCKTMPQYQKTKRIAFLWEDLAWTTEWRKGISYINLPTWEKLA
                     EDCGLEVVYSKAVKPRGTMYLPILQDIKKANADVIFFISSWFTDTESFAKQWSDSAAR
                     DIQVNLYGGVAQTQDYWKMTGGKALGVISSYTDLDDIPLTPKTIPLVKKAHARKIPMQ
                     IHVHIAYADIYHFKAAIERAKGTGNIKNLIKAMEDVETVYSLGKMKYETRKIKPFYHS
                     RVRVDAANPWKTYPGYYLQLIVQFQKDGKVAFLHESCEENEKLVAKWVDHKRYRTPAE
                     LRK"
BASE COUNT         2220 a         2559 c         2724 g         2263 t
ORIGIN      
        1 ccatcatgat catgcaggaa ccggcgtcgt tcaatccgga agcgttgccg gcggtaacgg
       61 tgccgtcttt cttgaaggcg ggcttgagtt tgctcagcga ttccatgctg gcatcggccc
      121 ggggatgttc gtccgtatca aagaggaccg tccccttttt tgtcttgact tcgacgggca
      181 cgatttcatc cttgaaccac ccgttttgaa tggcggcgca ggcccgctga tgactcatga
      241 gcgcccattc atcctgctcc tgacgggaga ttttatacag atcggcaata ttatcggccg
      301 tgacgcccat gtgatagccc agaagcgcgt ccaccaggcc gccgatcatc aaactgtcct
      361 gaatcttctc gccgtcgttc aaccggtatc ctttgcgggc gccaaacagc agatagggag
      421 cgttgctcat gctttcgatg cccacgaccg cgccaatgtc gatcttgccc agcatgattt
      481 cctgggaaag cagttcggag gcgcgcatgg aagaggggca ctgctgatgg accgtaaacg
      541 caaaggattc caccggcagt tccgcgccca gcgcaatgtg ccgggcgctg ttgcccggcg
      601 cgccggcctg gttgcactga ccggccacaa cttcctgaac cagatttctg tcaatgcccg
      661 ctttggcaat ggcggctttt aaaaccgtca cgcccaactg cacaggagtc aagcccgaaa
      721 gcgaccccat gtaatctccg atagccgttc ggacgccgcc gacaacaacc acttctttca
      781 taatgctacc tctctgatct ttttattttg agttttaaga atgaaaatat tgacgacctc
      841 gtaaaaagtc catttgtaga gctttgaata aaggccattt ttgtcatttc gaccgaaggg
      901 agaaatcttc aacaaacggc catccttaag atttctcgtc gctcacgctc ctcgaaccga
      961 catttttcaa agctctcatt tcggtccctt ttcgtcatac cggcgaaggc cggtatccag
     1021 tcattacaat aagttctgga ctccgacttt cgccggagtg acggtttgaa ttccttttta
     1081 cgggttcatc aatatcgacg ggcaatcggc cgcttctttc cattgcggcc cgctgcctga
     1141 aaatttctca aacactcttt tttgctttat cgcgctttta tcaagaattg ttttcttagg
     1201 caagaaatta ttgagcgaac ttttcgcaaa atggaattca cgctgttaat gaaaacatcc
     1261 ggaatctcct tccctactcc ggcttccccg ttttttccag ccggattttt tgacgcctcc
     1321 gtgccgtttt ccggacggga gacggcgccg cttttgtgaa aatatcgcca tgagaatttt
     1381 tttgagagaa ttttttgttg acaaagccgt taacttattt taaaaagtaa aaaaattttt
     1441 tcactataag tgccgatgtt tcggggtgcc ataacaaagc agctgaaaag cgcatgactg
     1501 ttgggagccg aatcctggaa cgcatgtcca agcggttttc ctgaaaagcg aaaagataaa
     1561 acttctgaaa gctttggagg ccttgtggca gcaatcgttt ttgcgttaaa acggattgcc
     1621 cggattcaga tcaacctgcc caattcccaa aaggatgaca caccgcccca cggccattca
     1681 ctaaccgtca gtcattttcc atttttcaaa aatctaccgt ccgcaatttt tccaacagcc
     1741 gccgggccgg aggtgctgaa ggtctgccgc cgttgcgttt ttgcgcggcg cgccggaggt
     1801 tttccgatcc ggacctgatc caggcgaagg aagtggttcc cgggagaggg ggaatcttct
     1861 cattatcgat ccattgaaaa tatttcgatt catccaatga gggtaaaaag gcatggcgac
     1921 gtacatcgag aatgttcttg tttacacgtt tgtaatgggg tccatttatc ttctgcttgc
     1981 ccttgggttt tccctgatct gcggggtgct gcggattttc cacctcggat acgcctatat
     2041 ctttccgctc acgatttacg ggacctggat gttcatgtcg gcgctggggt ggcccctcat
     2101 tccctccatt ctcgggatga tcgtcgttca gttcattgtt gccatcgcga tttacaaggg
     2161 gatgatcaag cgatacatga cgacggagga tgttttgctc accgcgctgc tgctgattgc
     2221 gctgattgtg gagcaggccg caaattactt ttatccgatt caggcggggg tttatctgga
     2281 aaccacgctg gccgaaggca cttatcagtt cggagacgcc gtggtgccca agcagctgat
     2341 catcggctcc ctgatcgcgc tggcgatgac cggcgccttt gttttcttct tcgtgaaaac
     2401 aaaaaccggc atggccatcc gcgctttgtc ccaggatatt tattcttcca agattgtcgg
     2461 catcaacgtc gagacgcttt atatgttcac catgatgctg gtgcttttgc cggtgattat
     2521 cggcacgctc attgttgcgc cggtctgggc gctggatccg gggatggggt ggatgtacat
     2581 ggtgacggca atcctggttg cggtgttggg cgggctgggc aacatcaaag gcaccattct
     2641 ggccagcttt ttgatcggat ttgcccacgc ccttgtctgt tttgtcctcg gagagccgcg
     2701 tttcatgaat ttgtcggcct tgattcttgt catggccatt ttgattattc gaccccaggg
     2761 gttagcaagg agtgaatcgc tatggtagaa acggaattaa aacgggaccg catcgtcgtc
     2821 cggctgggaa ccaaaaaatg ggaatggacc ctggaacctc gttactggcg caacacgctg
     2881 atctgggccg tcgtcctttt tatctttccg acggtatcct acttcatcag ccccggcctg
     2941 atcaacacca tgatttcggc caatatctat gccgccatcg ccatgccgct ggcgctcatg
     3001 accatcggca cggggcgaat gaattttggc ccccaatttt acatcggcgt aggagggtac
     3061 acggcggcgc ttctgagcat cgcctacgga tggggcccgc tggccacttt tcccttcgcc
     3121 gtcctgatgt ccatgatttc ggcgctgatt ttcagcccgc tggtcatcat ggcgcgcggt
     3181 ctttactatg tgctcctgac gctgctgctc ccgctggttt tcctggaagt gacctttatc
     3241 tatccggata ttttcaaagg cgataccggc ctcttcggca ttgtgccgct gttggacacc
     3301 ggaaacgcca aaaacaattt tttgctgatc ggctatattt ccaccgccat catgttcgct
     3361 tatttgtgga tcatcaacaa ggtcctgaaa tcccgctacg gcctcttgat ggcggcgatc
     3421 aatgacgatg aggacgtcgc ccattccatc ggcatcaaca tcaacaaaat caaaattatc
     3481 gcgtttgtct ccgcctccac gatgatcggc atcatcggat ggttttacgc gcactacttc
     3541 agttcatttg ccggcatcac gtatttaccg ctgacgttta tgctgaaagt cctgctggtg
     3601 ctgatgatcg gcggccgcgc ccaggttttc ggctgcgtcg tcggcggcta ttttgtcgct
     3661 ttcctggaga tgctgatgat ccggtacatg ggcccgatcc agccggtgct gtttcccatt
     3721 attcttctgg cgctgctgtt tattcttccg gaagggctgt tcggcattta tcgaaagcgc
     3781 cgttatcgcg aatatctgcc aaccattcac gtgcggaggt agaaagccat gactccattg
     3841 ttgaaagtga gcaatctggt caagcgtttc ggaggaatcg tggcggtcaa caacgtatcc
     3901 tttgaaattt acgaggggga aacactgggt tttatcggcc ccaacggcgc cgggaaatcc
     3961 accgtggtca actgcctgac gggttatctt cccgcgacgt ccggtaagat tgagatgaac
     4021 ggcgtggata tttcgcgcat gaagccgcat gagcgggcgc acatggggct tgcccgaacc
     4081 tatcagattc cgcgcccttt cccggaactg acggccctga ccagcgttct ggccagttcc
     4141 gtttcaggca agaaaagggt caaccagagt tttgaggatg caatgtacga tgcgacgcat
     4201 tacctggaat ttgtggggct gttcagcaag cggaatattc tggcgcgcga cctgaccttt
     4261 tatgaactga gaatgctgga attggcgcgt gccctggcca ccaccccgca gttgcttttt
     4321 atcgacgaag tcatggccgg cctgaatccc ggagaagcca aaaacgccat ccgaatgatc
     4381 cggaaagcca aggagcaatt cggcattacg gtcttctgga tcgagcatgt catggcggtc
     4441 ctgatggagg cggccgaccg gattgtcgcg atcaattacg gggccaaact ggcggaaggc
     4501 acaccggatc aggtggtgaa caatgatgag gtaatcgagg cgtatctggg cagaggttgg
     4561 agggaatatg cttgaagtca gtgatttgag cgcgtcttac ggcgtcattc cggcgctgca
     4621 cggcgtcagt ttcaatgttc ggcagtcgga aattgtaacg atcctggggt ccaacggaac
     4681 gggaaaaagc acgattctca acgtgatcca gggcatgatg accggcacca gcggcagcgt
     4741 gatctttcgc gacgaagcca ttcagggcct gccgtcccac aaaatcgttg acctgggcat
     4801 cgtgcaggtt ccggaaggcg cgaagagctt cccgtttatg acggtgaagg aaaatctgac
     4861 gctgggcgca taccggaaaa cgtcctggcc caaaagaaaa cagacgattc agtacgtgta
     4921 cagtcttttt ccgattttga aggagcgctc caacatgtcc gcccgcctcc tttccggcgg
     4981 cgagcagcag atgctgaaaa tcggccgggg gctgatggcc aatccgcatc ttctgatggt
     5041 ggacgaacct tccatcggtc tggccccgct cattgtggac gaagtctatg aaacactggg
     5101 gaagcttcgg aaaacgggac tgaccatttt attgaccgaa caaaacgccc tgcgcgcgct
     5161 gcaactggcc gaccgcggtt atatcattca ggagggaaga attgttctgg agggaagatc
     5221 ggcggagctg atgaaaaaca atctggtcaa aaaggcatat ttggggagat agcgatggaa
     5281 gattattttt cttatacggc aaaacttttc aatcccgatg aggcggaccg gaagccggaa
     5341 gcgctcaaag gcatccgggt gctggacttt tcccacgtca tcttcgggcc gatcgcggcg
     5401 aggctcctgg ccgattacgg cgcggaagtg atcaagctgg agctgccttt ttacggcgac
     5461 ctgtggcgtc cggcgacgta ctggggcaaa tactggaagc actcgaatcc catctggcac
     5521 aacatcacca aaaacaagta ttttctctcg gtgaacctgc agctcaagga atccaaagaa
     5581 ctgatctatc gaatggtcaa agacgcggac gtggtcattg aaaactttgc gcccgggacg
     5641 gcggatgcct gggacatcgg ctatgagaaa atttccaaga tcaattccag catcattttc
     5701 ctgagctgct ccacctatgg ccagttcggc ccgatgcggt atttccccgg gtgggacctt
     5761 ctggcccagg gcgcgagcgg cgtgctgacg ctgaacggct atccggacac ggacattttt
     5821 tacaagctgc cggactacgt gggcgacttc atgccgggga attttgccgc tttatcggtg
     5881 ttgatggcgc tgtaccaccg gaagaaaacc ggaaaagggc agtatatcga catggcgcag
     5941 acggaaggac tgatgaggac cctgtttcat tacacctatt attccgtgac tcaaaaagcg
     6001 atcggccgca ccggaaatgt cgatcccacc atggtgccgg cggccatttt taaaacggcc
     6061 gacgacaagt ttctggcgct ggcctgcgcg acgtccgagc agtttgaaaa ttttacccgg
     6121 gccatcggca ggccggagct gtcttcggat ccgcgttttt ccgatacgct ggagcggctg
     6181 aagccggaaa acgccaaagc gctccatgca attgcggtgg actggattcg gtcgcagtcc
     6241 tgcgccgatg tcatccgcct ggccgacgat aaaaatattc ctgcggcaga ggtggcggac
     6301 gattacatga tcgccaacga agaatggcgc cgcaagcgcg gaagcgtggt gcttttcaag
     6361 gatgatgtat atggcaactt ggtgattgcc gggccttccg cgatgctttc cggaacgcct
     6421 tcgcgaacca agtggctggc gcggccgctg gggtatcaca accggtttgt gctcaagaaa
     6481 tttctggggt tgagcgaagc ggaaattgaa aagctggaaa agaaaaaagt catcgggaca
     6541 tttgacgatc ggccgggact gaaaccgcct gtctattacg atctgagcaa ggatcccatc
     6601 tataactacg gaaaagaggt tggaaaatga gccccaggga agaagtttac actcggcacg
     6661 gcggcaaggg cggtgatcgg ggaaaaattc tcgccgaaat gatgaacgcc gaaggaaaac
     6721 cggaagtgct cgacgacatt ctggtgctgg atgtcagctc ggccaatttt gcgggcattg
     6781 tcgccgccag tttctttgcg gaattcggcg cggaggtcat caaaatcgag cctccggaag
     6841 gcgaccctgc ccgcctgatc acgccgttcg gcgtgacggt tcagggggtg ggcgttccct
     6901 tcatgttcga gggccgcaac aaacggtaca tgacgctcga cgttaaaaac aatcccgaag
     6961 acaggaaggt ttttgcccgg ctgtccaaaa aggcggcggt cgtgattgaa agttttgcgc
     7021 ccggcgagat ggacggctgg gggatcggct accgccagct tgtcgcggag aatccggggt
     7081 tgatttacat cgcgctttcc gcctacggac agtacacgga caaggccagg gaatccgcga
     7141 aaatgccgga tacggacatt acgtcccagg cgggttcggg gcttcccgcg cagatgggcg
     7201 cgccgcccaa tgagccggag ccctatacgt ggcctctgcg cggcggcatg tgggcggggt
     7261 ggtatatctc cgggctttcc gctgccctgg gcggcgcggt cgccctgctc caccggcaaa
     7321 acaccggcca ggggcagatg gtggatgtcg ccggaatgga cgcgtattcc tccatggtcg
     7381 gcatgcccgc caccatcggt tttacctggg aaaaagcccg cccgcgcatc ggcgttctgg
     7441 acttcattct ctatccctac ggccactgga agtgcaagga cggctacgtg gcgatcgccg
     7501 cgccgcggga ccacgatttc cgggcgctct tgaaggttct gggtctgtgg gaacaggagg
     7561 acgactggcg ctattcgtcg gatcgaatcc ccgatattct ggagcaggcc atccatctgc
     7621 ataagatcat cgaagagaaa acactgcagt ttacagccga cgagctggtg aagatggcgc
     7681 tcaattacag caaaaaatcc gcccgctcca agtggcgggg cggcggcgtg cccattgtca
     7741 tgaagctgat gacgccggaa gaagcgatgg agaataaaca gtggcacatc cgcaaggcgt
     7801 ttcaggaggt ggaagacaaa aaactgggca aatacacgat cagctccaat tttgtaaaaa
     7861 tgtccgaatc tcccccccgg gtcaaatggg tgggaagcga aatcggcggg gacaatgaat
     7921 atatccggaa aaaatattta aacgattaga aggatcaaat gttcggcgga tatttgatcg
     7981 tacattttta aagggttggt ttgacaaaca ggaggggaaa catgatgaag agagcaagca
     8041 gagtcaaaat ggtcgctggg gcggcgctgc tactgctggt tgcgattgct atcagcgtac
     8101 catccgttgc ggtggcgcag ccgaagggag agccgtttgt catcggctac atcggcatgg
     8161 tcggatcgcc tggcacccgt ccgtgcatgg acatccagaa aatggcggtg gaggaaatca
     8221 acgccgccgg cggcatcctg ggacggccgg tcaagtatgt cgtcatggac aacaagggcg
     8281 acacgtcgct gtccgtggag gcgtttcgca aactggtcat cgaggacaag gccaaattca
     8341 tctccgtcga aggccggacg gaagtctgtc tggccgttca ggaagcttcc ggaatgctgt
     8401 tcaaggaata cccccacatc ctcattttca acgggccgat gggcgcggaa ctgacggcgc
     8461 gcatcatcga cgaagcgccg aaatacaatc actgtttccg cgactgggat ccggagccgg
     8521 cgcattatgc ccagacgaag tatttcttct gcaagaccat gccccagtat cagaaaacga
     8581 aaagaatcgc cttcctctgg gaagacctcg cctggaccac ggaatggcgc aaaggcatca
     8641 gttatatcaa cctgcccacc tgggaaaaac tggccgagga ctgcggtttg gaagtggtgt
     8701 acagcaaggc ggtcaagccc cgcggcacca tgtacctgcc gattcttcag gacatcaaga
     8761 aagccaacgc cgatgtgatt ttcttcatca gctcctggtt tacggacacg gaatcctttg
     8821 ccaaacagtg gtcggattcc gcggctcgcg acatccaggt caacttatac ggcggtgttg
     8881 ctcagaccca ggattactgg aagatgacgg gaggtaaagc gttgggtgtt atcagttcct
     8941 atacggacct ggatgatatt cccctgacgc ccaaaaccat tccgctggtt aaaaaagcgc
     9001 atgcgcggaa gatccccatg cagatccatg tccacattgc ctatgccgat atttatcatt
     9061 tcaaggcggc catcgaaagg gcgaagggaa ccggcaacat taaaaacctg atcaaagcca
     9121 tggaagacgt ggaaaccgtc tattcgctgg gcaagatgaa atatgaaacc cggaaaatca
     9181 aacccttcta ccattcccgt gtgagggttg acgcggccaa tccgtggaaa acctatcccg
     9241 gttactacct gcagttgatt gttcagttcc agaaggacgg aaaggttgcc ttcctgcacg
     9301 agtcctgcga ggaaaacgag aaactggtgg ccaaatgggt ggatcataaa cgctatagaa
     9361 cacccgcgga gttgaggaag taatggacgg cagggaaccg tcgcgacggt tccctgcagg
     9421 ctgaagaccg aagtctgaag gctgaaagga aagggacggc attcttgtga aggccgcccc
     9481 tttttctttc ccgccacggg gctgccgttt ccggcccgcc gcgcccctga aatgccgctt
     9541 gacacccccg ctggttattt catatacttc acgccgcaaa aacaaaggga gagctttgaa
     9601 aaatgtcatt tcgaggagcg tgagcgacga gaaatcttaa ggatggccgt ttgttcaaga
     9661 tttctccctt cggtcgaaat gacaaaaatg gccgttattc aaagctctcg gaggctgcgg
     9721 tggcgctggt tttgcaggcg gccgatcacg ggttcgaagg aggaaa
//
LOCUS       DAOSRM010000084         9752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_6730_length_9752_cov_95.996184, whole genome shotgun sequence.
ACCESSION   DAOSRM010000084 DAOSRM010000000
VERSION     DAOSRM010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6730_length_9752_cov_95.996184"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..3109
                     /locus_tag="PLA58_08215"
     CDS             101..3109
                     /locus_tag="PLA58_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HOR62816.1"
                     /translation="MIGKTQGVFQHLIIIFLLLAAVWVSAAERASFGMIVKPVSDEYA
                     RFIGLDGPEGAYVVSTPASGGLRPGDVILKVDDAPIKTPADLQEVIRVHQPGDQVQLM
                     IARGKERVQISYALKSAASSGAGFRPGQAARMPSGLGSFPTIESRDFEKEVLKSERPV
                     LAYFYANWCVPCKTYLPIMQEIGQQHADVKIVGIDVDKSRDIVNRYGISGILPAVILF
                     NGGREIDKVMRVSKKALVDGLLTKIRKDGDEIEVFPSIGKGHWITDVAFIKDSGWLTS
                     RDSDQTIFIWDYRQGALVRTYRAVKSALSPNGAYAALADKNRTAIAVVDIAKQKQSLI
                     QTKLFIEKLAISPFGNAIAGFGAEQNGRFSVNIWTPDNRLIRSIPVDPENNPLMVQFA
                     FSPDGASFALSTLNKLEFFATENWERKTVVTMKGAARVLQFTSDSRAILAPGVDENLV
                     DLQGRKTRGIGGRTVVGRPDARLLIVDNGDNSFRLMSDKSGGTGLRFAGHRAPVNAGA
                     VGDAVRWLASGSSDGTVRLWDPATGDEMVQLLAFGNGEWIVITPEGYYNASARGHEFL
                     NIRRGSRVYGMDQFYDVFYRPDIVSAKLKGDPIAGLVTLTVEEAIKNPPPAVQISFLP
                     DRTGDAKVKVCYRVQSTGGGIGEVRLFQNGKLIKSDGFYREVASQDMMATLKLTQLDG
                     GTVYRDMRSLTIREKQSPGAVVLRAKGDVFEECLEMETIAGENEIGLAAFNASNTVQS
                     AMSTARFLSTRASDEPHLYILAVGIDRYRDASINLKYAAKDARDFLAQLADKAKTIYK
                     PDNIHLTRLIDERAGKSDILKAVEKLASEVKHGDSFIFFNASHGLLLQSQYYIVTGSF
                     DGSLNRSESLISSNEIVEMSKKIKSLSQLYIFDTCHAGGVDAIVSGLYDARMSVLAKK
                     MGLHIYASAGSVETALDGYQGNGLFTHALLEGLKNGAAVDKDKSGAVTVKSLGIYARE
                     TTREISNTLGHPQTPLMINFGKDSRLFEVR"
     gene            3162..4022
                     /locus_tag="PLA58_08220"
     CDS             3162..4022
                     /locus_tag="PLA58_08220"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOR62817.1"
                     /translation="MRKILFVLALLILTAAPVYAAQSTIKIAEGSACMGDDKSRKQTE
                     TAALAEAKRNAVESVKTYVSSATEVKDFELQKDLVSAYSNATVEVLETLEKSWYKDPG
                     QGDCYRIKLKAEVIPDEKAMKKAGAKASDAALDDPSLPLTVKVWTDKTSYAAGEKIRI
                     YLKGNKPFFARVLYRDAAKNTLQLLPNPYRQDHYFQGGVIYEIPSGKDRFELEVNPPF
                     GEENIIVYASVSELGDLKLKDEGSVFAVQTRSKDIGVKTRSVKIKAASDGAGQAAEFS
                     EVKAVVKTRK"
     gene            4038..5831
                     /locus_tag="PLA58_08225"
     CDS             4038..5831
                     /locus_tag="PLA58_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOR62818.1"
                     /translation="MKKVSILFLSFLMLAGFLAVVQDGKAQSPEPLKAVLIFDMGGRG
                     DGGFNDSAYSGMEKAVAELGVQAVYIEHKRNLDLDHAVNEAAASDAQVIIGVGFAFSG
                     KFDHLAVRHPDKKFVVVDYSVKFDERGNLSAPPPNLAGLTFKEEEGSCLVGAIAALKS
                     QTGKIGFIGGMDSPIIRKFQAGYEAGAKAVRPGIRIHSQYAGITGQAFRDPQKGYRIA
                     ARMYKNGADVIYHAAGETGAGLFRAAREMNRLAIGVDIDQSAQAPGRVLTSMLKNIDG
                     AVFDVVQSCVRGNFSGGLKTLGLKEHGVGFVYNDQNKKLIPESIHQQVQALQAKIVSG
                     EMTVPVASGHRTVLSRQELRDLLTRLQSEIQTALNKLDNDLRHSAKVLAGRDLQGDFA
                     RGILKGLYRANPYIIDCETVSDAGIMVAVEPPAHRSSEGADISRQAHMVRLFKTHKPV
                     LSGSFLSVEGPRSVVIHHPVFSTDREFSGSVSALFAPEYLLSSMIGPIAANIPVSIFL
                     MQTDGLFIYDVDTKQIGLNIFTDPLYQPFPELIKLCRRMAGAREGSGVYHFYRKVGEA
                     PITKVVEWRTVELHGTPWRLAVAFAKDGIGR"
     gene            complement(5874..7706)
                     /locus_tag="PLA58_08230"
     CDS             complement(5874..7706)
                     /locus_tag="PLA58_08230"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HOR62819.1"
                     /translation="MSTQPVKIPTTLSASEFLLKIIEIAPRLPSMIFNLIKGLKSIGS
                     NTNNSWGLELERIAAQYPQNAAVKSPDGTLTYQELNERANRFAHYLISKGVRRGDVVT
                     LCFENRPELLAAYCGCSKVGAIASLINTNQRGDSLVHSFNLNRGKVVLIGEECCEFFI
                     EARSGIDLSQSTLGMVRDAGQFKTKDVPDITDELARQNGANPSTTKSVTLKDTMAYVY
                     TSGTTGGLPKAAVIINKRTLSAMFWFGKFVMPVKPTDTIYIPLPFFHTNAITVGWPPA
                     LYNGAAVALRRKFSASKFLDDVRRFQATHFVYVGEVCRYLMATPAKPDDHQTTLKYIC
                     GNGLRPDIWMPFKKRFGIDKIYEFYGAAEGVGVFTNFLNIDYSVGTSVTPFAIVAYDT
                     ENDRPVKDEKGFLKKVGKGETGLLIMEISEKTPFAGYSDKKKTEEKIFRDVFRKGDLW
                     FNTGDMLRNMGFRHAQFADRLGDTFRWKGENVATQEVEKAIDTFPGVKQSAVYGISMP
                     GSDGKAGMAAVIRDENAAIDLSRLVAHLKTGLPKYAVPIFIRFVPDFEWTATHKIKKT
                     NLKNEGYDPARVREELYVLLPGSETYVPVSEEIYRNITAGTYKF"
     gene            complement(7727..8176)
                     /locus_tag="PLA58_08235"
     CDS             complement(7727..8176)
                     /locus_tag="PLA58_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62820.1"
                     /translation="MRARTILFFAFLFAVMPAWLSAEKMTGNWRLTGYTKYRDAVFVD
                     RDRISQPSHDLTAAWIKIAPSGKSRHLRLVAEYMKSVQKWNPQFKSVEILCEINCSRD
                     FIRFKEFVYLDKDRNVLHKAHETGTQWFLINPGSTWHFVKKESCAIQ"
     gene            8394..8663
                     /locus_tag="PLA58_08240"
     CDS             8394..8663
                     /locus_tag="PLA58_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR62821.1"
                     /translation="MNRAELVEEVAKVTCTKKEADVAISAALAAIQKALKKGDSVTLV
                     GFGTFSVSKRKARKGRNPQTGEAIKIAAKKVPVFKAGKGLKDAVK"
     gene            complement(8749..>9752)
                     /locus_tag="PLA58_08245"
     CDS             complement(8749..>9752)
                     /locus_tag="PLA58_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR62822.1"
                     /translation="VPRVFNRIYDGLWAKMNKEGGLAKKLFVMGVEAGKKKRELAARG
                     QSDFLTELKFRIADKIVFSKIREKLGGRVKGAMTGSAAMNPEIGHFFFDIGLPVYDCY
                     GLTETSPAATMNASYAYRLGSVGRAIEKVRIVIDSSVVEEGATDGEIIVYGPNVMKGY
                     HNRPEDTAAAMTPDGGFRTGDRGRLDKDGYLFITGRIKEQYKLENGKFCFPVSIEEEI
                     CLLPLVQQAVIYGYNRPFNICIVVPDFDVLKNTAKEKGLPTDINELVERQDIVDMYGA
                     AITAHLKNKFGGYEIPKKFLLIPEPFTLENGTLTQTMKLKRKVVLDKFQDRIEALYR"
BASE COUNT         2193 a         2478 c         2855 g         2226 t
ORIGIN      
        1 ggtcgtttcc atcaacggcc aggcgtttcc cgtgggaaaa gtaaaatgag caatctttct
       61 tcaaacgagc agaaaccgtt ttttgtcaac gagaggattc atgatcggta aaacgcaagg
      121 agtgtttcaa cacctcatca tcattttcct gctcctggca gccgtgtggg tgtcggctgc
      181 cgagcgggcg tcgttcggca tgatcgtcaa gccggtatcg gacgaatatg cccggttcat
      241 cggtctggat gggcctgaag gcgcttacgt cgtttcaacg cctgcctccg gcggtctgcg
      301 tccgggggat gtcatcctga aggttgacga cgcgcccatt aagacgcctg ccgatcttca
      361 ggaggtcatc agggttcacc agccgggtga tcaggtgcag ttgatgattg cgcggggtaa
      421 agagcgcgtt cagatcagct atgccctgaa gagcgccgcc tcttcaggcg caggttttcg
      481 ccccggccag gccgctcgaa tgccttcggg attgggctct ttccccacca tcgaaagcag
      541 ggactttgaa aaggaagttt tgaagtccga acggccggtg ctggcgtatt tctacgccaa
      601 ctggtgcgtt ccctgtaaaa cgtatctgcc catcatgcag gaaatcggcc agcagcatgc
      661 agacgtaaaa attgtcggca tcgatgtcga taaaagccgc gatatcgtca atcgctacgg
      721 catttcagga attctgccgg cggtgattct gttcaacggc ggcagggaaa ttgacaaggt
      781 gatgcgcgtg tcgaagaagg cgctggtcga tggactgctg acgaaaatac gcaaagacgg
      841 ggatgagatt gaagtgtttc cctctatcgg caaaggacac tggatcaccg atgtcgcttt
      901 tatcaaggat agtggatggt tgacttccag ggacagtgat cagacgattt ttatctggga
      961 ttatcggcag ggagccctgg tgagaaccta tcgggcggtc aaatccgctt tgtcgcccaa
     1021 cggagcgtac gcggcgcttg cggataaaaa tcgaaccgca attgctgtgg tggatattgc
     1081 aaagcagaag caaagcttga ttcagacaaa actatttatt gaaaaattgg cgatatcccc
     1141 gtttggcaat gcgattgccg gtttcggcgc tgagcaaaac ggccggttca gcgtcaatat
     1201 ctggaccccg gacaacaggc tcattcggtc cattccggtt gatcctgaaa acaatccgct
     1261 gatggtgcag tttgcatttt caccggacgg cgcctccttt gcgctttcaa cgctgaacaa
     1321 actcgaattt ttcgccacgg agaactggga gcgcaaaacg gttgtgacga tgaaaggcgc
     1381 ggcgcgggtt ttacagttta cttccgacag ccgggcgatt ctggcgccgg gggtggacga
     1441 aaatctcgtt gatctgcagg gtcggaagac ccgtggaatc ggaggacgca cggtcgtcgg
     1501 caggcccgat gctcgtctgc tgatcgtgga taacggcgac aactcctttc ggctgatgag
     1561 cgacaagtcc ggcggcacgg ggcttcgttt tgccggccat cgcgctccgg tcaatgcggg
     1621 agccgtcggc gatgcggtgc ggtggctggc cagcggaagc tcggatggga ccgtcaggct
     1681 gtgggacccg gcaaccggcg acgaaatggt gcaacttctg gcctttggca atggggaatg
     1741 gatcgtcatt acgcctgaag gatattataa tgcttctgcc cgggggcatg aattcctcaa
     1801 tatccgcaga ggcagcaggg tttacggtat ggatcagttc tatgacgtat tttaccgtcc
     1861 cgacatcgtc tcggcaaagc tgaaaggaga tcccattgca ggtctggtta ctctgacggt
     1921 ggaagaagcg atcaagaacc ctcctccagc tgtacaaatt tcttttcttc ccgatcggac
     1981 cggcgatgcg aaagtgaagg tttgttatcg ggtgcaaagc acgggcggcg gcatcggtga
     2041 ggtgcgtttg ttccaaaacg gtaaattgat caagtccgac ggattctacc gtgaagtcgc
     2101 atcgcaggac atgatggcaa ccctgaaact gacccagttg gacggcggga ctgtgtatcg
     2161 ggatatgcga tctctcacca tcagggaaaa gcagagcccc ggcgccgtcg ttctgcgggc
     2221 aaagggtgat gtattcgaag aatgtttgga gatggaaacg attgccggtg agaacgagat
     2281 cgggcttgcc gccttcaatg cttccaacac agtgcagagt gcgatgagca cagcccgttt
     2341 tctgtccacc cgcgcgtccg atgagcctca tctttatatt ctggccgtcg gcattgaccg
     2401 gtatcgcgac gcctccatca acctgaaata tgcggcaaaa gatgcccggg acttccttgc
     2461 ccagctggcg gataaggcca aaaccatcta caagccggac aacattcacc tgacccgcct
     2521 gatcgatgag cgtgccggca aatccgacat tttaaaagcc gttgaaaaac tggcttcgga
     2581 agtaaaacac ggcgatagct ttattttttt caatgcgtct cacggcctgc ttttgcaaag
     2641 ccagtactac attgtcaccg gcagtttcga cggaagtctg aacaggagcg aaagcctgat
     2701 cagctccaac gaaattgttg agatgtccaa aaagatcaaa tctctgtcac agctttatat
     2761 ttttgatacc tgccacgcgg gaggcgtgga tgccattgtc agcggtctgt atgatgcccg
     2821 gatgtccgtg ctggcgaaga agatgggcct gcacatctat gcctcggcgg gctcggtgga
     2881 aacggccctg gacggttatc agggcaacgg attgtttact catgctcttt tggagggatt
     2941 gaaaaacggc gcagccgtgg acaaggacaa gagcggcgcc gttaccgtta aaagcctggg
     3001 aatttacgcg agagagacga ccagggaaat ttccaataca cttggccatc cgcaaacgcc
     3061 attgatgatc aacttcggca aagacagccg gttgtttgaa gtgcgatgaa gagaacgtga
     3121 ggcggattgc aattacgctt agccaattga aggaggtatt catgcggaag atattatttg
     3181 ttttggcttt gctgattttg acggcggcgc cggtttacgc cgcgcaatct accattaaga
     3241 ttgcggaggg ttccgcctgc atgggggacg acaagtcgcg caagcagacg gagaccgcgg
     3301 cgctggctga agcaaagcga aacgccgtcg aatcggtcaa gacctatgtc tcgtccgcga
     3361 cggaggtgaa ggacttcgaa ctgcagaaag acctggtgtc cgcctactcc aatgccacgg
     3421 tcgaagttct tgagacgctg gaaaagagct ggtacaaaga cccggggcag ggcgactgct
     3481 accggatcaa gctcaaagca gaggtcattc cggatgaaaa ggcgatgaaa aaagccggcg
     3541 caaaggcttc ggatgcggcg ctggatgatc cgtctctgcc tttgaccgtg aaggtttgga
     3601 cggacaaaac gtcttatgcc gcgggagaga agatcaggat ttatctcaaa ggcaacaaac
     3661 ctttcttcgc gcgcgtgctt taccgggacg cggcgaaaaa taccctgcag ctcctgccca
     3721 atccctaccg gcaggatcac tattttcagg gcggcgtgat ttacgagatc ccttccggca
     3781 aggatcggtt tgaactcgaa gtgaatcctc ctttcggcga ggaaaacatc attgtctatg
     3841 ccagtgtctc cgagctgggc gatttgaagc tgaaggacga gggaagcgtt ttcgccgttc
     3901 aaaccagatc gaaagacatc ggcgtcaaaa cacggagcgt caagatcaag gccgcgtcgg
     3961 acggcgcagg acaggcagcg gaattttccg aagtcaaagc ggtggtgaag acgagaaagt
     4021 aagagggaga gagaaaattg aaaaaagtct cgatattgtt tttgagtttt ctgatgctgg
     4081 cgggctttct ggcggtcgtt caggacggga aggcgcagag cccggagccg ctgaaggcgg
     4141 ttctgatctt tgatatgggc ggccgaggag acggcgggtt taacgattcc gcgtacagcg
     4201 gcatggaaaa agcggttgcc gagctgggtg tgcaggctgt ctatatcgaa cataaacgaa
     4261 acctggatct ggatcatgcc gtaaacgagg ccgcggcttc cgacgcgcaa gtgatcatcg
     4321 gcgtcggctt tgccttttcg gggaagttcg accatctggc cgtccggcat ccggataaaa
     4381 aatttgtcgt ggtggactac agcgtgaaat tcgatgagag gggaaacctc agcgcgccgc
     4441 cgccgaatct tgccggactg acgtttaagg aagaggaagg gtcctgtctg gtcggggcga
     4501 tcgcggccct gaaaagccag acggggaaaa tcggttttat cggcgggatg gacagcccga
     4561 tcatccgcaa atttcaggca gggtatgagg cgggcgcaaa agccgtccgg ccggggatcc
     4621 gcatccattc acaatatgcc ggcatcaccg gacaggcctt ccgggatccg cagaaaggtt
     4681 accggatcgc ggcgcgcatg tataagaacg gagcggacgt catttatcat gccgccggcg
     4741 aaacgggagc gggcctcttc cgggcggcga gggagatgaa ccgtctggcg atcggcgtgg
     4801 acatcgatca gagcgcgcag gcgcccggcc gcgtcctgac gagcatgctg aaaaacatcg
     4861 acggggcggt attcgacgtt gttcaatcct gcgtccgcgg aaatttttcg ggaggcctca
     4921 agacactggg gctgaaggaa cacggcgtcg gatttgtgta taatgaccag aacaagaagc
     4981 tgattcctga aagcatccat cagcaggttc aggcgctgca ggcaaaaatc gtgtcgggcg
     5041 agatgacggt tcccgtcgcg agcggacacc ggacggtgct gtcccgtcag gaattgcgcg
     5101 acctgttgac ccgtctgcaa tccgagattc agacggcttt aaacaaactg gacaatgatc
     5161 tgcggcacag tgccaaggtg ctggccggca gggatcttca gggagatttt gcgcgtggca
     5221 ttctcaaggg cctttacagg gcaaacccct atatcatcga ctgcgagacc gtgagcgatg
     5281 caggaatcat ggtggcggtg gagccgccgg cgcaccggtc gtccgaaggc gccgacatta
     5341 gcaggcaggc gcacatggtc cggctgttca aaacccacaa gccggtattg agcggctcat
     5401 tcctttccgt cgaaggcccc cggtccgttg tcattcatca tccggtattc tcgacggatc
     5461 gagaattttc gggatcggtt tcagccctgt ttgcgccgga atacctgctt tcgagcatga
     5521 ttggaccgat cgccgccaac ataccggttt ccatttttct gatgcaaacc gacggtcttt
     5581 ttatatatga tgtagatacg aaacagatcg gtctcaacat cttcaccgat ccattgtacc
     5641 agccgtttcc ggagctgatt aaactttgcc gcaggatggc cggcgcccgc gaaggatcgg
     5701 gtgtttacca tttttaccga aaagtcggcg aggcgccgat aaccaaagtc gtggaatggc
     5761 gcaccgttga actgcacggc acgccctggc gccttgccgt tgccttcgcc aaagacggca
     5821 tcggccgtta aaaattgatg aacccgcaat aagccgggct ttttacgaag ccgttaaaat
     5881 ttgtacgttc cggccgttat attgcgatat atttcctcac tgacgggcac ataggtttcg
     5941 ctccccggca gaagcacata cagttcctcc ctgacccgcg ccggatcgta gccttcattc
     6001 ttgagattgg tcttcttgat tttgtgcgtc gcggtccatt caaaatccgg aacgaaacgg
     6061 atgaaaatcg gcacggcgta tttgggcagg ccggtcttca ggtgggccac cagccgggac
     6121 agatcgatcg ccgcattttc atcccgaatg acggcggcca ttccggcctt tccgtcgctg
     6181 ccgggcatgg agatgccgta aaccgcagac tgcttgacgc cggggaaggt gtcgatcgct
     6241 ttttcgactt cctgcgtggc cacgttttcg cctttccagc ggaacgtgtc gcccagacgg
     6301 tcggcgaact gcgcgtgacg gaaacccatg tttcgaagca tgtcgccggt gttgaaccac
     6361 aggtcgcctt tgcggaaaac gtctcggaat attttttctt ccgtcttttt cttgtccgaa
     6421 tacccggcga agggcgtttt ttcggagatt tccatgatca gaaggccggt ttcgcccttg
     6481 ccgacttttt tcaaaaagcc cttctcgtct ttgacgggcc ggtcgttttc ggtgtcgtag
     6541 gccacgatgg cgaacggcgt cacgctggtg cccacgctgt agtcgatgtt caggaagttc
     6601 gtgaaaacac ccaccccttc ggccgcgccg taaaactcgt atattttatc aatgccgaac
     6661 cgttttttga acggcatcca gatatccggc cgaagcccgt tgccgcagat gtacttcagc
     6721 gtcgtctggt ggtcgtcggg ctttgcgggc gtggccatca gatagcggca gacttctccc
     6781 acgtaaacga aatgggtcgc ctgaaaccgc cggacgtcgt ccaggaattt gctggcgctg
     6841 aattttctgc gcagggctac ggccgcgccg ttataaagcg ccggcggcca tccgacggtg
     6901 atggcgttgg tgtggaaaaa gggaagcgga atataaatcg tatccgtggg tttgacgggc
     6961 atgacgaatt tcccgaacca gaacatggcc gacagggtcc gcttgttgat gatgacggcc
     7021 gccttcggca gtcccccggt ggttccggag gtgtaaacat acgccatggt gtctttcagt
     7081 gtgacgcttt tggtggtgga gggattcgcg ccgttttgcc gcgccagctc atccgtaatg
     7141 tcggggacgt ctttggtctt gaattgcccc gcgtcccgga ccattcccag cgtcgattga
     7201 gaaagatcga ttccggagcg cgcctcgatg aaaaactcgc agcattcctc gccgatcagg
     7261 acaactttgc cccggttgag gttgaagctg tgcaccagag agtctccccg ctgattggtg
     7321 ttgatcagcg aggcgatggc tccgaccttt gagcaaccgc agtaggcggc cagcagctcg
     7381 ggacggtttt caaagcaaag cgtcaccaca tcgccgcggc ggacgccttt ggagatcaga
     7441 tagtgggcaa aacggttggc ccgctcgttg agctcctggt aggtgagggt gccgtcagga
     7501 gacttgaccg cggcgttttg cgggtattgg gcggcaatcc tttccagttc cagcccccag
     7561 gagttgttgg tattgctgcc gatgcttttc aatcctttga tcaggttgaa gatcatcgac
     7621 ggaagccgtg gtgcaatttc aatgattttc agaaggaatt cggacgcgct tagggttgtg
     7681 gggatcttta ccggttgagt gctcatagtt tcttctccct gtttttttat tggatggcgc
     7741 aagattcttt ttttacgaaa tgccaggtgc tgccggggtt gatcaggaac cattgcgttc
     7801 ccgtttcatg ggccttgtga agaacgtttc tgtctttgtc cagatagaca aattccttaa
     7861 aacggatgaa atctctggaa caattgattt cacaaaggat ttcgacggat ttgaattgcg
     7921 gattccattt ttggacggat ttcatgtatt cggcaacgag gcgcaggtgc ctgctttttc
     7981 cggacggggc gatcttgatc caggccgccg taaggtcatg agagggttgt gaaatccggt
     8041 cgcggtccac aaagacggca tcacggtatt tcgtgtagcc ggtcaaccgc cagttgccgg
     8101 tcattttttc cgcggacagc caggccggca tgacggcaaa taaaaaggcg aaaaataaga
     8161 tagtcctggc gcgcatgatc cttcccttca gattcatttt ccctttacag caaaacatgg
     8221 gaaatttgaa gagcggatat gcgcttcggg cggaaaaacg gccggaaagc gcttcaggcc
     8281 tcaaaataca cgaaaataat cggatgaatt ttacaaaaaa cagtagattt ttttttgaat
     8341 taaggttaat gagtcaccat cgttttacaa aataaaattt aaaggggtga gcaatgaaca
     8401 gagcagaatt ggtcgaagaa gtagcaaaag tgacgtgcac gaagaaagag gctgacgtgg
     8461 ccatcagcgc tgcgttggcg gcaattcaga aggcgctcaa gaagggcgac agcgtgacgc
     8521 tggtgggatt cggcaccttc tctgtcagca agagaaaggc ccgcaaaggc agaaaccccc
     8581 agacaggcga agccatcaag atcgccgcca agaaggttcc ggtattcaag gccggaaagg
     8641 gtttgaagga cgcggttaaa taactttcat ttgtcatcat tgaaaaaaag ccggctcgcg
     8701 taaaaaggag ccggcttttt ttattgttcc tgcgggacac ggatcctgct accggtacag
     8761 cgcttcaatc cgatcctgaa atttatccag cactactttg cgtttgagct tcatggtctg
     8821 ggtcagcgtg ccgttttcca gcgtgaacgg ttcgggaatc agcaaaaact ttttggggat
     8881 ctcgtagccg ccgaacttgt ttttgagatg agccgtgatc gcggcgccgt acatatccac
     8941 gatatcctgt ctttcgacca gctcgttgat gtccgtcggc agtccttttt cttttgcggt
     9001 gtttttcaga acgtcaaagt cgggaacgac aatgcaaatg ttgaaggggc ggttgtatcc
     9061 gtaaatgacc gcctgctgaa ccagcggcaa caggcagatt tcttcttcga tggatacggg
     9121 gaagcagaac ttcccgtttt ccagtttgta ctgctccttg atgcgtcccg tgataaagag
     9181 gtagccgtcc ttgtccaggc ggccgcggtc gccggtgcgg aagccgccgt ccggcgtcat
     9241 ggccgcggcc gtatcttccg gccggttatg gtagcctttc atgacattgg ggccgtaaac
     9301 gataatttcg ccgtcggtgg cgccttcctc caccaccgaa gaatcgatca cgatgcggac
     9361 cttctcgatg gcccggccga cgctccccag gcggtaggca taggaggcgt tcatggttgc
     9421 agccggcgac gtttccgtga gcccgtagca gtcgtaaacc ggcaggccga tgtcaaagaa
     9481 gaaatgaccg atttcaggat tcatcgcggc gctgccggtc atggcgccct tcacccggcc
     9541 gcccagcttt tcgcggattt tcgaaaagac gattttatcc gcgatcctga atttgagttc
     9601 ggtcagaaaa tcggattgac cccgcgcagc cagctcgcgt tttttcttcc ccgcctcaac
     9661 gcccatgacg aaaagttttt tggccagacc gccttccttg ttcattttgg cccagaggcc
     9721 gtcgtagatg cggttgaaaa cgcggggcac gg
//
LOCUS       DAOSRM010000085         9692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_6776_length_9692_cov_89.005915, whole genome shotgun sequence.
ACCESSION   DAOSRM010000085 DAOSRM010000000
VERSION     DAOSRM010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6776_length_9692_cov_89.005915"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..717
                     /locus_tag="PLA58_08250"
     CDS             142..717
                     /locus_tag="PLA58_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOR62823.1"
                     /translation="MKAFIEPPESIPFYLKIGLWISKKATGRDLLPGKLLAWYPRTAI
                     SSGVMEALVAHQDKNLNKRMLKLVRLRTSFAVACPFCIDMNSYDYDQFGISPEEFSAL
                     QGRIAIATVPTFSPRERIAMEYAFLISQSPLLFPQIFIDQLKTNFTEREMVILAGTAA
                     QVNYWARLLQALGVPPAGFSDHCEMKTQPSS"
     gene            complement(729..1772)
                     /locus_tag="PLA58_08255"
     CDS             complement(729..1772)
                     /locus_tag="PLA58_08255"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOR62824.1"
                     /translation="MAEKKEAPFPLSLEAPVPEIYMPRLQLFVRLLLGALVALYINYS
                     PVPPLVLSVNQVNLSLVFYYAFHLFWWWYYRKYGANIIMFRLGGWIDILGAFIGALSD
                     PFMIPPMILLFLIAALGNGIQHGLYFFVESMIGSLILAGAVLVIHCRILGDWPPYHLY
                     FYVFLIVVGVSYAYLLVRRIEMMRGEAVRISEHDSLTGMRNRRSFLKAAQYLLLLNER
                     SNISLVLMFADLDNFKTVNDQFGHEMGDKVLRHFSDMARSSFRKSDILARYGGDEFVM
                     LLTNTSPEAAQSAARRLQGEFGAWAKENGLQTGVSFGLAAISKGKNNLDDLLRQADAA
                     LYEAKPKEGLKGF"
     gene            complement(1765..2682)
                     /locus_tag="PLA58_08260"
     CDS             complement(1765..2682)
                     /locus_tag="PLA58_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR62825.1"
                     /translation="MEDFSVDPVTPADSTYTTASVCVQKGVHLRVMIWRPKQPAIANP
                     IIFVAGWVSDVSGWAPLLEVMAVGRPVYYIETREKRSALFEQSKLKPEDFSIGRMAED
                     LIVACRQLGIASPASTVIGSSLGATALLEALKGGRLIAGEAFLIAPNSEFKAPWYLQW
                     MLCLPAFLYHGIKYFLLWYLRAFMVDAKKEPEQMMRYTETLKAAHPQRIKLSARAAIE
                     GRYQVWPNLDSVRVPVALAYAPTDTLHSSANIRLMAERLPRSTVVACPSNRHMHGAEV
                     MQDIEAFILREKSAAMMQAPEDNRRCKDG"
     gene            complement(2717..3226)
                     /locus_tag="PLA58_08265"
     CDS             complement(2717..3226)
                     /locus_tag="PLA58_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HOR62826.1"
                     /translation="MMKKLALVTGAAILLLILLLALWLISRPDRGTTGAVSTQFRWIG
                     PNDKIVVDGFDDPKVQGVACHIARAQTGGVKGALSVAEDASDASIACRQIGPIKFLKE
                     FKDGEQVFDEQRSLLFKSLQVVRFYDRKRNVLVYLSYSDRVLTGSPKNSISTVPVMPW
                     PPPETGAVK"
     gene            complement(3320..5476)
                     /locus_tag="PLA58_08270"
     CDS             complement(3320..5476)
                     /locus_tag="PLA58_08270"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOR62827.1"
                     /translation="MAIHENIYRDEHGIPHVQCRSKEDLFRGQGYVHATDRGMQMILM
                     RTLGQGRAAELLDPGDEMVGIDRFFRKMNWYGEIEEELEKLPGDIKQYLGAYCDGANA
                     AFSNKFPWELKLLGMKFEPWKIEDIIQISRMIGYLTLSQSQAEMERLFVEMVQAGVSR
                     EKLEELFPGILGGLDIELVKKVVLNERIVPNSRLWNIALPRMMASNNWVLSGKKTVSG
                     KAVLSNDPHLEVNRLPNVWCEMFFEMEDRFLYGGTMPGVPGIFVGRSNDLSWGATYTF
                     MDAEDSWIEKCKDEKFYREEAGWMPFGKRKEIIKRKNRQPVEVVFYENGHGVLDGDPS
                     VEGLYLTTKWATSRSGADTITNVLKLLDATTVEEGMNCLGKVESSWNFVLADSKGNIG
                     YQMSGLMPKRRDGVSGFVPLPGWIKENDWKGFVSFDQLPRVINPEGGFFVTANHDLNE
                     YGKAKPINMPMGPYRAERIAQILSRDKKFSVEDIFSMHMDVFSVQAEAFMKILKPLLP
                     ADGNGRILTDWDLRYDPGSEGAYLFEEFYKELYREVFGENNLGTHVVDFLKKETGAFV
                     DFYLNFDRILLMEKSLWFNGRKREDLYKKSLGKVLSMKPKKWGDVQKVMLRHILFGGK
                     LPAFLGFDRGPVTIIGGRATIHQGQIYRSANRETTFAPSFRFVTDFAREEFFSSIAGG
                     PSDRRFSKWYASGLEDWTQGKYKTLKVNPERKSKFI"
     gene            5638..8418
                     /locus_tag="PLA58_08275"
     CDS             5638..8418
                     /locus_tag="PLA58_08275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR62828.1"
                     /translation="MSIPTLTLTPSGQVIFLPGGGDSPALPDAAAARIAAAFHEDAAA
                     GLLHLAAEELTTPLPPDFAFWRDFSRRCLTQLCRIAAVESEGPDFAAPPPSPEDLLRI
                     VETSPPLQGLEYLSPAVLENLWTALQDRTRSEAGKAGGSVQDYLKNRNPLWNMVGRVV
                     FHLAENRKNEERPFGFLITYTTRLSGLAKPQFLPLGKAFREFASAQDRGGLLALLTPL
                     QRAAEASALIRELVDSGQIFHPLAWTVRETCRFLDDIPRFESSGILVRIPEAWRDRGR
                     PPRPQVSVTVGARQSGGLGLDALLDFSVGLTLDGEELTGEERRRILSETSGLVLLRGK
                     WVEIDREKIAALLSHWEKRKEAEAGEGISFTEGMRLLAGAELADRDALPDAEASREWL
                     NADAGEWLRSILAGMRSPEGMIPADPGPALKTQLRPYQMTGVAWLRFMHDLGLGSCLA
                     DDMGLGKTIQILALLLILRQRKASESPSSSGPAILIVPASLVANWKAEIGRFAPSLVA
                     FYAHPSETPAADLAVMARAEGRSATLSEADLIVTTYAMVHRMAWLRETVWGLIILDEA
                     QAVKNPGARQTRAVKSLKGKRRIILTGTPLENRLGDLWSLFDFLSPGLLGTEKVFSRY
                     VKGTPDRKDNAYAALRALVRPYILRRLKTDRRIIADLPDKTEVRVFCPLTKTQAALYE
                     ESVREMAGKLKEARGIQRRGLVLAYLMRFKQICNHPSQWLGDQAYGTGASGKFQRLRV
                     LCEEIASRQEKLLVFTQFREMTEPLAALLAETFLRPGLVLHGGTGVGKRREIVEAFQR
                     EEGPPFFVLSLKAGGVGLNLTAASHVIHFDRWWNPAVENQATDRAFRIGQKKNVLVHK
                     FVCRGTVEEKIDALIEDKKELVESILGGDREKLVTEMPDEELIRFVSLDLSRALDEEN
                     NGKKKMDRRNSG"
     gene            8415..>9692
                     /locus_tag="PLA58_08280"
     CDS             8415..>9692
                     /locus_tag="PLA58_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62829.1"
                     /translation="MSWGRRRYDWGRWPRYVPVAERREKARKQAEKLRKKGGSLSPVI
                     LAGRTIAATFWGKAWCQNLERYRDYENRIPRGRAYVRNGSVLDLKIGAARVDALVSGT
                     HLYSVTVEIRPLKAQVWKEVKKACTGRIGSLLELLQGKLSREVMEVVTRPQTGLFPEP
                     AEISFRCSCPDWASMCKHVAAVFYGIGARLDREPDLLFRLRDVDHRELIAAPDAADLA
                     ASPGMGVKTIAEEDLAGIFDIEIDEGQTAGQRVTRTTPKPGKSVKIRAVSIEAVAPKE
                     DHAPGSAITGGTQAQTRNQRTFKSKEQEAATPSEPLKRKLGRPRLVQEDVSLPKPRRG
                     RPPLVRKGPTTTQQARKSFAISEKQATGRKPSRKSRPMPPTQSPEPAKPRRSRPPKAA
                     AAAVSPSAPPKRKRGRPLGGGNARHETKERDGQP"
BASE COUNT         2114 a         2873 c         2594 g         2111 t
ORIGIN      
        1 ttaagtattt cagttgatgc cgatatgctg gttttttcgc tgcaatcgat tgaagttccg
       61 gccactttga gataggtgat actttaagaa tatcccttat gtccatgatt ttcataatat
      121 ttcagtgtta aggagagaat catgaaagcc ttcatcgaac cgccggagtc tatccccttt
      181 tatctgaaaa tcggcctctg gatttctaaa aaagcgaccg gtcgtgatct tttgccgggg
      241 aaactgctgg catggtatcc cagaacagcc atcagttccg gcgtcatgga agccctggtt
      301 gcccaccagg ataaaaattt gaataagcga atgctcaagc tggttcgact gcggacatct
      361 ttcgcggttg cctgcccttt ctgcatcgat atgaattcct atgactatga tcaatttgga
      421 atctcaccgg aggagttttc cgcattacag ggaaggatcg ccattgcaac cgttcctact
      481 ttttccccgc gcgaaagaat tgcgatggaa tacgctttcc tgatttcaca atcccctcta
      541 ttgttcccgc aaatttttat tgatcaactg aagacgaatt ttaccgaacg ggaaatggtc
      601 attctggccg gcacggccgc gcaagttaat tactgggccc gcctgcttca ggcgctgggc
      661 gtccccccgg cgggcttctc ggatcactgc gagatgaaaa cgcaaccttc atcgtgacag
      721 gccatcgatt aaaacccctt caagccttcc tttggttttg cctcatataa cgcggcatcc
      781 gcctggcgca ggagatcatc aagattattt ttgcctttgg atatcgcagc aaggccgaaa
      841 ctgacgccgg tctgcaatcc gttctctttt gcccaggcgc cgaactcgcc ctgaagcctt
      901 cgagccgccg attgcgcggc ttcaggcgag gtgtttgtga gaagcatgac aaactcgtcg
      961 ccgccgtatc tcgcaaggat atcggatttc ctgaagcttg accgcgccat gtcggaaaaa
     1021 tgccggagaa ccttgtcgcc catttcatgg ccaaactggt cgttgacggt tttgaaattg
     1081 tcaagatcgg caaacatcaa gaccagggaa atattggatc gttcgttgag gagcagcaga
     1141 tattgagcgg ctttgagaaa tgaccgcctg ttgcgcatcc ccgtgagact gtcgtgttcg
     1201 ctgattctga cggcctcacc cctcatcatc tcgattcgtc gcaccagaag ataagcatag
     1261 cttacgccga caacgatcag aaagacataa aaatacagat ggtagggagg ccaatcacca
     1321 agaatacggc aatgaatgac cagaacggcg ccagcgagaa taagcgaccc gatcatcgat
     1381 tcaacgaaaa aatacaaccc gtgctggatt ccattgccaa gcgccgcaat caggaaaagc
     1441 agaatcatgg gagggatcat gaagggatcg ctcagggccc ctatgaatgc ccccaggatg
     1501 tctatccatc cgcccagacg aaacatgata atgttcgccc catacttcct gtaataccac
     1561 caccagaaga gatggaaagc ataatagaac actaacgaga gattgacctg gttgacagat
     1621 aacacgagcg gtgggacggg gctgtaattg atgtataacg caaccagagc tccaagcagc
     1681 aggcggacaa ataactgaag gcgcggcatg tatatttctg gaacgggcgc ctcaagggac
     1741 aggggaaacg gagcttcttt cttttcagcc atccttgcac ctccggttat cttccggggc
     1801 ctgcatcatt gcggcgctct tttcccggag gatgaacgct tcaatgtcct gcatcacctc
     1861 cgcaccgtgc atatgtctgt tggacggaca ggcaacgacc gtggaccgcg gcagtctttc
     1921 cgccatgagg cgaatatttg cggaagagtg cagcgtatcc gtgggagcgt aagccaacgc
     1981 cacagggact cgaacgctgt ccaggttggg ccagacctgg tagcgcccct caatggcggc
     2041 tctggccgaa agcttgatcc tctggggatg ggccgctttc agcgtttcgg tataacgcat
     2101 catctgttcg ggttcctttt tggcgtcgac catgaaggcc ctcaggtacc agagaagaaa
     2161 atatttgatt ccatgataca ggaacgccgg aaggcacagc atccactgta aataccatgg
     2221 cgccttgaat tcactgttgg gggcgattaa aaacgcctct cccgcgatca gccgcccccc
     2281 tttcagagcc tccagcaggg ccgtcgctcc cagggagctg ccgataacgg ttgaagccgg
     2341 cgaggcgatg cccagttggc ggcaagcgac gatcaggtct tcagccatcc ttccaatgct
     2401 gaaatcctcc ggcttcagtt tgctttgctc aaagagggcg gatctctttt cccgtgtttc
     2461 aatataataa accggccttc cgacggccat gacctccagc aagggcgccc atccggatac
     2521 gtcggatacc catccggcta caaagatgat cgggttcgcg atggcgggct gtttcgggcg
     2581 ccatatcatg acccgcaagt ggacaccctt ctgtacgcaa accgacgctg ttgtgtaagt
     2641 tgaatccgcg ggtgtaaccg gatccacgga gaaatcttcc aaatcggcct cctttgcgga
     2701 acggatacaa tatttttcac ttcactgcgc ctgtttccgg gggcggccag ggcatgacgg
     2761 gcacggtgct gatgctgttt ttcggactgc cggtcagcac acgatcgctg taagacaaat
     2821 agaccagaac attgcgtttg cggtcataga agcgcacgac ctgaagcgac ttgaaaagaa
     2881 ggctgcgctg ttcgtcaaaa acctgttcgc cgtctttaaa ttcttttaga aacttgatcg
     2941 gtccgatctg gcggcaggcg atgctcgcgt cgctggcgtc ctccgccacg cttaaagcgc
     3001 ccttgacacc cccggtttgg gcccgggcga tatggcaggc cacgccctgc accttgggat
     3061 catcaaagcc gtccacgacg attttatcgt tggggccgat ccagcgaaac tgggtgctga
     3121 cggccccggt tgttccgcga tcgggccggc tgatcagcca taaggctaaa aggagaatca
     3181 gcaataaaat cgctgcgccg gtaaccaacg cgagcttttt catcatcggt cgtcctttct
     3241 tccgaaagtt cttttgttat gcctcacaaa cacatcgctt gggcgatgaa atgtcaagaa
     3301 taaatatccc tatttacact cagatgaatt tactttttct ttcaggattg actttcagtg
     3361 ttttatattt cccttgcgtc cagtcctcaa gaccggatgc ataccatttt gaaaaccttc
     3421 tgtcgctggg accgcctgca atcgacgaga aaaattcctc tcttgcaaaa tccgtaacaa
     3481 atctgaaaga cggtgcaaac gtggtttccc tgttcgcgct cctgtaaatc tgcccctgat
     3541 ggatggtggc ccgcccgcca ataatcgtga cagggcccct gtcaaacccg aggaatgcag
     3601 ggagttttcc gccaaataaa atatgcctca acatcacctt ctggacatcc ccccatttct
     3661 taggcttcat cgacaatact ttccccagtg atttcttata gaggtcttcc cttttcctgc
     3721 cgttaaacca taaggatttt tccatcagca agatcctgtc aaaattcagg tagaaatcaa
     3781 caaatgcgcc cgtctccttt ttcaggaaat ccaccacgtg cgtccccaga ttattttcac
     3841 cgaacacctc ccggtaaagt tctttgtaga attcttcaaa aagatacgca ccctcggacc
     3901 cgggatcgta tctcagatcc cagtcagtca atatcctgcc gtttccgtca gcaggcagaa
     3961 ggggcttcaa tattttcatg aaagcttcag cctgaacgga gaatacatcc atgtgcatgc
     4021 tgaaaatatc ctccacggaa aactttttat ccctgctcaa tatctgcgcg atccgctcgg
     4081 ccctgtacgg ccccatgggc atattgatgg gttttgcttt tccgtattca ttgaggtcgt
     4141 ggtttgcagt gacaaaaaat ccgccttcag gatttatgac ccggggaagt tggtcaaagc
     4201 tgacgaatcc tttccagtca ttctccttga tccatccggg cagcggcaca aacccgctga
     4261 ccccgtcacg ccttttcggc atcaatccgc tcatctgata cccgatattg cctttgctgt
     4321 cggctaatac aaaattccac gaactctcca ctttacccag gcagttcatc ccttcctcaa
     4381 ccgttgtggc atccagcagt ttcagtacgt tggtgatcgt atccgcccct gatctgctcg
     4441 tagcccattt tgtcgtcaga tagaggcctt cgaccgacgg gtccccgtcc agaacgccgt
     4501 gaccgttttc ataaaaaacc acttcaaccg gctgccggtt tttccttttt ataatttcct
     4561 ttcttttccc aaaaggcatc cagcctgcct cttccctgta aaacttttca tccttgcatt
     4621 tctctatcca ggaatcctcg gcatccatga aagtgtacgt cgcaccccag gataaatcgt
     4681 tagaccttcc cacaaaaatt cccggaactc ccggcatggt gccgccatag agaaacctgt
     4741 cttccatctc gaaaaacatt tcgcaccaga cattgggaag tctgttcact tccaggtggg
     4801 gatcattgga cagaacggct tttcccgaaa ccgttttctt gcccgaaagc acccagttgt
     4861 tcgaagccat catccgggga agggctatat tccataaccg ggaattcgga acgatccttt
     4921 cgttcaagac aaccttcttc accagttcaa tgtccagtcc gcccagtatc cccggaaaca
     4981 actcttcaag cttttcccgg ctgacgcccg cctgaaccat ttcaacgaac aatctctcca
     5041 tttccgcctg ggattgagaa agcgtcaggt agccgatcat ccttgaaatc tggatgatgt
     5101 cctctatctt ccatggctcg aatttcatgc ccagcagctt cagttcccag ggaaatttgt
     5161 ttgagaaggc ggcatttgcc ccatcacaat atgctcctaa atattgcttg atatcccccg
     5221 gcaatttttc taattcttcc tcgatctccc cgtaccagtt catctttctg aaaaacctgt
     5281 caatcccgac catttcgtcg ccgggatcca ggagttcggc ggccctgccc tgtccgagcg
     5341 ttctcatcag gatcatctgc atgcccctgt cggtcgcgtg gacgtagccc tgtccccgga
     5401 ataaatcctc ttttgatctg cattgcacat ggggtatgcc gtgttcatcc cggtagatat
     5461 tttcatgaat agccattgga aagcctccgg atgtattttg tgccgcaaga ataccacaaa
     5521 caatacccct gttcaatccg gcataaacac aataaaccat gtactccctt gacttgttgc
     5581 cggctcttct ctatactcgc gccgttttgc aaatcccgaa agttcggatc gacgcccatg
     5641 tccattccaa cattgacact caccccctcg gggcaggtca ttttcctgcc cggcggcggg
     5701 gacagccccg cgcttcccga cgcggccgcc gcacgcatcg cggctgcctt tcacgaggac
     5761 gctgccgccg gcctcctcca ccttgccgcc gaggagctga ctacgcccct cccgcctgac
     5821 ttcgctttct ggcgcgactt ttcccgccgc tgcctgacgc agctctgccg gatcgcggcc
     5881 gtggaatcgg aagggcccga ctttgccgcc ccgcccccct ccccggagga tctcctcagg
     5941 atcgtcgaaa cctctcctcc gctgcagggg ctggaatacc tgagccctgc ggtcctggaa
     6001 aatctctgga cggcgcttca ggaccgcacc cggagcgagg ccggaaaggc gggaggaagc
     6061 gtccaggact atctgaaaaa ccgcaacccc ctctggaaca tggtaggccg cgtcgttttc
     6121 catctggcgg aaaaccggaa gaacgaggag cgtcccttcg gcttcctcat cacctatacg
     6181 acccgccttt ccggccttgc gaaaccgcag ttcctcccgc ttggcaaggc ctttcgggaa
     6241 ttcgcctccg cacaggaccg cgggggcctg ctcgctctgc tgacaccgct tcagcgggcg
     6301 gccgaagcga gcgcgctgat ccgggaattg gtcgattcgg gccaaatctt ccaccccctt
     6361 gcctggacgg tccgggagac ctgccgtttt ctcgatgata ttccgcgctt cgaatcatcg
     6421 ggcatcctgg tccgcattcc ggaagcgtgg cgggaccggg gccgtccccc tcgtccgcag
     6481 gtgtccgtga cggtgggcgc cagacagtcc ggcggcctcg gcctggacgc ccttctcgat
     6541 ttctccgtcg gcctgacgct ggacggtgag gaactgacag gagaagagcg acggcggatt
     6601 ctgagcgaga cctcgggtct cgtccttctc cgggggaaat gggtggagat cgaccgggag
     6661 aaaatcgccg cgcttttgtc gcactgggaa aaacgtaagg aagcggaggc aggagagggg
     6721 atctccttta ccgagggaat gcgcctcctt gccggggccg aattggcgga cagagatgcg
     6781 ctgcctgacg cagaggcatc ccgggagtgg ctgaacgccg acgcggggga atggcttcgg
     6841 tcgatccttg cggggatgcg ttctcccgaa gggatgatcc cggccgatcc cggaccggcc
     6901 ctgaaaacgc agctccgtcc ctaccagatg acgggggtcg cctggctccg tttcatgcat
     6961 gacctggggc tgggatcctg cctggccgac gatatgggtc tcggcaagac cattcagatc
     7021 ctggcgcttc tgctcatcct ccgtcaacgg aaagcctcgg agtccccttc ttcttccggg
     7081 ccggccatcc tgatcgttcc cgcctccctt gtggctaact ggaaggcaga aatcggccgc
     7141 ttcgccccgt cgctcgtggc tttctatgcc catccatccg aaacgcccgc ggccgatctg
     7201 gctgtgatgg cgcgcgcaga aggccgcagc gcaacgctct cggaggcgga tctgatcgtc
     7261 accacctatg cgatggtcca ccgcatggcg tggcttcggg aaactgtctg gggactcatc
     7321 attctggatg aggcgcaggc ggtcaaaaac ccgggggcaa gacagacgcg ggccgtcaag
     7381 tcgctcaagg gaaaacggcg gatcatcctt accggaaccc cgctggagaa tcgtctcggg
     7441 gatctgtggt cccttttcga ttttctttct cccggcctcc tggggactga aaaggttttc
     7501 agccgctatg taaaagggac gcccgaccgt aaggacaacg cctatgccgc cctgcgcgcc
     7561 ctggttcgcc cttacatcct caggcgtctg aagacggacc ggcggatcat cgctgacctg
     7621 ccggataaaa cggaggtccg ggtcttctgc ccgttgacga aaacccaggc cgccctctac
     7681 gaagagtccg tccgggagat ggccggcaag ctgaaggaag cccgcggcat tcagcggcgg
     7741 ggacttgtcc tggcctatct gatgcgcttc aagcagatct gcaaccatcc ctcccagtgg
     7801 ctgggggatc aggcttacgg aaccggggcg agcggcaagt tccagcgcct ccgcgttctc
     7861 tgcgaagaga tcgcctcccg gcaggagaag ctcctcgtct tcacccagtt ccgcgaaatg
     7921 acggagccgc tcgcggcgct cctggcggag acgtttctgc ggccgggtct cgtcctgcac
     7981 gggggcacgg gggtcggaaa gcggcgggag atcgtcgagg cctttcagcg ggaggaggga
     8041 ccgccttttt ttgttctttc cctgaaggcc ggcggggtcg ggctgaacct gacggcggcc
     8101 tctcacgtga ttcattttga ccgctggtgg aaccctgcgg tggaaaacca ggctacggac
     8161 cgggcgtttc ggatcggtca aaaaaagaac gtcctcgtcc acaagttcgt ctgtcgcgga
     8221 accgtggagg aaaagatcga cgccctgatc gaggacaaga aggagctggt cgagagcatc
     8281 ctcggcggcg acagggaaaa gctcgttacc gagatgccgg atgaagaact gatccggttc
     8341 gtctctctcg atctttcccg cgccctggac gaagagaata acggcaaaaa gaaaatggac
     8401 aggaggaaca gcggatgagt tggggaagaa gacgctacga ctggggaaga tggccgcgct
     8461 atgttccggt ggcggagcgg cgcgaaaagg cccgcaaaca ggcggaaaag ctgcgaaaga
     8521 agggaggttc gctttctccg gtgatcctcg ccgggcggac catcgccgcc actttctggg
     8581 gcaaggcctg gtgccagaat ctggaacgat accgcgatta cgagaaccgg attccgcgag
     8641 gccgcgctta cgtgcgcaac ggttcagtcc tcgacctgaa aatcggcgcc gcccgtgtgg
     8701 acgccctggt cagcggcacc cacctctatt cagtgaccgt cgagatccgc cctctgaagg
     8761 cgcaggtctg gaaggaggtc aaaaaagcct gtacggggag aatcggatcc ctcttggaac
     8821 tgcttcaggg aaagctgtct cgggaggtca tggaggtcgt cacccgcccg cagacaggac
     8881 tcttccccga gcccgccgag atatccttcc gttgttcctg cccggactgg gcatccatgt
     8941 gcaagcacgt cgccgcggtt ttctacggca taggcgcccg cctcgatcgg gaaccggatc
     9001 tgctcttccg cttgcgcgac gtggaccatc gggagctgat cgccgcgccc gatgcggcgg
     9061 atcttgcggc ctcgcctggg atgggggtca agacgatcgc ggaggaggat ctggccggga
     9121 tcttcgacat cgagatcgac gagggacaga cggcgggcca gcgggtcaca cggactacac
     9181 ccaaaccggg caagagcgtg aaaatccgcg ccgtatcaat tgaagccgtt gcgccaaaag
     9241 aggatcacgc tcccgggtcg gcaatcaccg gagggacaca ggcgcagacc aggaaccagc
     9301 ggacgttcaa atcgaaggag caagaggccg cgacgccctc cgaacctctg aagcgcaaac
     9361 tgggcaggcc ccggctggtg caggaggacg tctctttgcc caaaccccgc cgggggagac
     9421 cgcctctggt ccgcaaaggg ccgacgacga cgcaacaggc gcgaaaatca ttcgccattt
     9481 cggagaagca ggcgaccggc cgcaaaccaa gtcgaaaatc ccgtccgatg cccccgacgc
     9541 aatcgcccga accggcaaag ccccggcgct ctcgcccgcc gaaagcggcc gctgccgccg
     9601 tctccccctc cgcaccgcca aaacggaaac ggggccgccc gctgggggga gggaacgcaa
     9661 ggcatgaaac gaaagagagg gatggacaac ca
//
LOCUS       DAOSRM010000086         9185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_7215_length_9185_cov_105.347536, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000086 DAOSRM010000000
VERSION     DAOSRM010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7215_length_9185_cov_105.347536"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLA58_08285"
     CDS             <1..244
                     /locus_tag="PLA58_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62830.1"
                     /translation="SDAEKGSYLKTIPGTVPGLYNLPVGCRFADRCALAEDRCRASEP
                     ALAELEAGHFVSCFVAMQEKARHCEQTLGLRGNLNA"
     gene            345..1313
                     /locus_tag="PLA58_08290"
     CDS             345..1313
                     /locus_tag="PLA58_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR62831.1"
                     /translation="MASVLMDIQRLKKAYVLGGGFLRQKPRVIRAVNGVDLQIFEGET
                     LGLVGESGCGKSTLARLLMKLEEPDEGSACFRGQNIASFSPDDLKTYRRRVQMIFQDP
                     YSSLNPRKSARSIISEPLTIFRTSNRAGRKEKTLQLMKKVGLSEEQAGRYPHEFSGGQ
                     RQRIGIARALALQPELIIADEPVSALDASIQAQILNLLRDLKKDFGLTYLFISHDLNV
                     IHHVSDRIAVMYLGRIVELADKNELYREPLHPYTRMLMAAMPGCVPQRKKREMTVLGE
                     AGDAGETGCSFKSRCIYKIDVCEQHAPDLRPCSGSRLCACHRAEEI"
     gene            1425..2045
                     /locus_tag="PLA58_08295"
     CDS             1425..2045
                     /locus_tag="PLA58_08295"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOR62832.1"
                     /translation="MAKEEKVFWPNGSLTGEGLWHQAAGDAGAVICHPHPLMGGSMHN
                     NVVETVRDEMIAAGHSTLRFNFRGVGKSTGHYDEGRGEKQDLLSACAFAKSRGVEKIF
                     LVGYSFGAWICCRLLKDHPSVASFVVLISPPLKYFEFDWTGLENAVELMICGDQDMFC
                     DVADLRKKAELICAKLTILRGVDHFYFGQEARLAVHLRRYICEKKA"
     gene            2268..2468
                     /locus_tag="PLA58_08300"
     CDS             2268..2468
                     /locus_tag="PLA58_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOR62833.1"
                     /translation="MAEGKVKWFNEKKGFGFIENDEGGDVFVHYSAIQGTGFKTLYEG
                     QRIRFDIEQGNKGPSAANVQPL"
     gene            2533..3444
                     /gene="miaA"
                     /locus_tag="PLA58_08305"
     CDS             2533..3444
                     /gene="miaA"
                     /locus_tag="PLA58_08305"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOR62834.1"
                     /translation="MKKNLIVILGPTASGKTAMAARLAAEFGGEIISADSRQVYRGMD
                     IGTGKDLEEFRIDGRVIPCHLIDILDPDQEFNVFEFQKRFYEIFSALREKKVLPLLVG
                     GTGLYLESVLTRYVLPEAEPDERIREELAGLTMGELQEMLLAMKPQLHNQTDLHDRER
                     LVRKIEIEKARQNPSKSQTRFPQIQAGIFGIFWERAELRKRISARLHIRLAEGMIEEV
                     SRLHDEGVSWERLESFGLEYRFIARYLQKTMTRDEMAAGLQTAIGQFAKRQMTWFRRM
                     EKKGVAIEWIPGGDYPALASRVMKMLS"
     gene            3504..4859
                     /locus_tag="PLA58_08310"
     CDS             3504..4859
                     /locus_tag="PLA58_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62835.1"
                     /translation="MKKDSLFENYLNRYAAGLPWRLQAADSRACEQIVVIPAYAERDS
                     LFCTLASVAANPEAMLVKTMVLCVVNNRAASPAEDKENNAQTLAMLNALVRRQSFDIP
                     KADGKLREAWQAVAASPLRLGFIDATSPGLEIPDRAGGVGMARKIGMDAALRLLATSE
                     QKPRLILSLDADTLVRPDYLSGVRSAFASGRAQAGILDYEHQMPPDLAGQTAICRYEI
                     FLRYWVLGLQFACSPYAFHSIGSTMATTDEGYLSVRGMNRREAGEDFYFLNKLAKAGP
                     LRRIRETRVYPSGRASARVPFGTGAAVAKMVSGARSDFLLYDPRIFIVLRSWIALMKE
                     SFEDTADEILGKAKEIHPGLSEFLAARGFLTIQPRIRANVKDRRTYERQMHGWFDGFE
                     TLKLINGLTRDFYPKILLPAAVTQILEMQNLECPRRFSGESAPGSEDCMEMLSYLRGL
                     T"
     gene            5267..5611
                     /locus_tag="PLA58_08315"
                     /pseudo
     CDS             5267..5611
                     /locus_tag="PLA58_08315"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
     gene            5654..6184
                     /locus_tag="PLA58_08320"
     CDS             5654..6184
                     /locus_tag="PLA58_08320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR62836.1"
                     /translation="METGGKIKPTDKEIGKFYTDNKNKFTTEESVHVRHILVAMDAKD
                     DEKVKAEKKAKIEGLRKQIMGGADFAEVAKNNSDCPSKENGGDLGEIKKGQTVKPFED
                     AAFSQEIKAVGPVVSTEFGHHIIQVLGRTPAHTVSLGEVKEQISLYLEQQKQAEAFSK
                     MMARLKKNAVIQYGNP"
     gene            6466..7356
                     /locus_tag="PLA58_08325"
     CDS             6466..7356
                     /locus_tag="PLA58_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR62837.1"
                     /translation="MRIRQAGRIDDGLWLLGTQESCVYLLEGTKTSVLISAGMPYILP
                     DLFRQMASRGMTPEKIEQVVILHAHFDHVGVVPYLKRNRPDMKIYASARGWEVLTNPR
                     AVSVINEFSEKVRRRMKGSEDSLAGLDWAWRDDVKGETLAQGDRLDLGGRQLQFFETP
                     GHSSCSISAWVPGLNILFPSDAVAIPYRDEYIIAVGSSFEKYLASLDKLAGLDVRKIC
                     ADHYGCVTEEEARGYIAQTRTATLQMMDRMREALAREKSVEEAARQLIRLHYELRPDY
                     FVHPDILLRTYTQMLKQLTG"
     gene            complement(7362..8180)
                     /gene="panB"
                     /locus_tag="PLA58_08330"
     CDS             complement(7362..8180)
                     /gene="panB"
                     /locus_tag="PLA58_08330"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOR62838.1"
                     /translation="MSTQTKINRKTILDIKKMKTQGEKIAMLTAYDFGMASILDESDI
                     DILLVGDSLGNVVLGYDTTLPVTMEDMIHHTRAVARGSQKALIVADMPFLSYQVSQEA
                     AMANAGRLLKEGNAQAVKLEGGGEHAEIVRKMTLSGIPVMAHLGLTPQSIHQLGGYKV
                     QGKKEDAAEKMMRDALILEEAGAFSVVLECVPEKLAAEITSTLTIPTIGIGAGVSCDG
                     QVLVVNDMLGMNDRMTPKFVKKYAHLSQEIKNAAKRYASEVKTGAFPDADHSFK"
     gene            complement(8177..9067)
                     /locus_tag="PLA58_08335"
     CDS             complement(8177..9067)
                     /locus_tag="PLA58_08335"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOR62839.1"
                     /translation="MKRKKPPLTFAVVGAGMVGTAIGFLLGKAGRHVAAVADCSAAHR
                     KRAASYLRAPGFPHPAQAAALADCVLITTPDDRIASVCAEIAARTGLKGKNVFHMSGA
                     GGLDLLAPAARAGALTASIHPLQSFSSIDGAIASIPGSYFGVTADAGVKRLAADIVRD
                     LQGIPIPISAAQKPLYHAAACIASNYLVSLLSVVESIYMSIGFSEKDARRAYLPLVYG
                     SLKNIENQGCPNALTGPIARGDSGTVQKHIDAMARHLPDFASMYAHMGAVAVKLAREK
                     GTLSAAQAKEILGMLKGAKS"
BASE COUNT         2125 a         2348 c         2705 g         2007 t
ORIGIN      
        1 aagcgatgcg gaaaaggggt cctatttaaa aaccattccg ggaacggttc ccggcttgta
       61 taatctcccc gtcggttgcc gctttgccga ccgctgcgcg cttgccgaag accgctgccg
      121 ggcgtcggag cccgcgcttg cggaattgga agcggggcat tttgtgtcct gctttgttgc
      181 tatgcaagaa aaagcccgtc attgcgagca gaccctcggt ctgcgtggca atctaaatgc
      241 ataagattac cacgacgcct gagaggcgtc tcgtaatgac caataagaat taagatatag
      301 tctcccacta aaggagaaca ttaaaaagat tattcaaggt gtccatggct tccgttttga
      361 tggatattca gcgactgaaa aaagcctacg ttttaggcgg cgggtttctg cggcagaagc
      421 ccagggtcat tcgggcggtg aacggcgtcg atcttcagat cttcgaggga gaaacgctgg
      481 ggctcgtcgg cgagtcgggg tgcggcaaat ccacgctggc gcgcctgctc atgaagctgg
      541 aggagccgga tgaggggagc gcttgtttca gaggccaaaa cattgcatcc ttttcgccgg
      601 acgatttgaa aacgtaccgg cggcgcgtgc agatgatttt tcaggatcct tattcttccc
      661 tgaaccctcg aaaatccgcc cggagcatta tttccgaacc gctgacgatt ttccggacgt
      721 ccaaccgcgc cgggagaaag gaaaagacgc ttcaactgat gaaaaaagtc ggcttgagcg
      781 aagagcaggc cggccgctat ccccacgagt tcagcggcgg acagaggcag cgcatcggca
      841 tcgcccgggc gctggcgctg cagccggagc tgatcatcgc ggatgagccg gtttccgcgc
      901 tcgacgcttc catccaggcg cagattctga atctccttcg ggatctgaaa aaagatttcg
      961 gcctgaccta tctgttcatc tctcatgatc tcaacgtcat tcatcatgtg tccgaccgga
     1021 ttgccgtgat gtatctgggg cgcatcgtag agctggcgga taaaaatgaa ctttaccggg
     1081 aacctttgca tccctacacc cgaatgctga tggcggccat gccgggctgc gttccgcaaa
     1141 gaaaaaaaag ggagatgacg gttcttggag aggcgggcga cgcgggggag acggggtgca
     1201 gttttaaaag ccgctgcatt tacaagattg atgtttgtga gcaacacgcg ccggatttac
     1261 gaccctgttc gggaagcagg ctttgcgcct gccaccgcgc ggaagagatt tgatcaatgt
     1321 agggaacggt cgcgaccatt ccctgcgggt catgggagat gggtgatagg caatgggcga
     1381 tgggtgtaag gaacaatgta gtgcggaatg atggagggtt ctccatggcg aaggaagaaa
     1441 aagtattctg gcccaacggc agcctcaccg gcgaaggatt gtggcatcag gcggcaggcg
     1501 acgcgggcgc ggtgatctgt cacccgcatc ctttgatggg cgggtccatg cacaacaatg
     1561 tcgtggaaac cgtccgcgat gaaatgatcg ccgccggcca ctccacgctc cgttttaatt
     1621 ttcgcggcgt cgggaaaagc accggacatt atgacgaggg caggggagag aagcaggatc
     1681 ttctgtccgc ctgcgcgttt gccaaaagcc gcggcgttga aaaaatattt ctcgtcggtt
     1741 attcgttcgg cgcgtggatc tgctgccgcc ttctgaaaga tcacccgtcc gttgcttctt
     1801 tcgttgtgtt gatttctcct ccgctgaaat acttcgagtt cgactggacg ggtctggaaa
     1861 atgctgtgga attaatgatc tgcggggatc aggatatgtt ctgcgacgtc gctgatctca
     1921 gaaaaaaagc agaattgatt tgtgcaaaat tgacgatcct tcggggtgtc gaccattttt
     1981 atttcgggca ggaggcccgt ctggctgtgc atttgcggcg gtatatttgt gaaaaaaaag
     2041 cttgatttta aaaaaaatgg tgttatgttc gagtccaacg caccggtttt atagccggtt
     2101 ttgcaatggt tgcagggttt gggttggtaa atacgtagtg ttgaaatcga tagacaaaag
     2161 aaaataaccg caaagagttc ttcttgaaaa taagaattct tgcggttttt tagtttctga
     2221 cgggatcacc cggaagaagc aaaattttga aaggggaggg tttagcagtg gcagaaggca
     2281 aagtgaagtg gtttaatgag aagaagggtt ttggcttcat tgaaaatgat gaaggtgggg
     2341 atgtttttgt acattacagc gccattcagg gaacgggctt caagactctc tacgagggac
     2401 agaggattcg tttcgacatc gagcagggga acaagggccc cagcgcggca aacgttcagc
     2461 cactttagtc gctgaaaaag atgaacaaaa aaatgccccg tgatgcgggg catttttttt
     2521 gatccgtaca tcatgaaaaa aaatctgatc gtcatcctgg gaccgacggc atcgggaaaa
     2581 acggcgatgg ccgcaagact ggccgccgag tttggaggtg aaatcatttc cgcggattcg
     2641 cggcaggttt accggggcat ggacatcggc accggcaagg atctggaaga attccggatc
     2701 gacggccgcg ttattccctg ccatctgatc gacattttgg acccggatca ggagtttaat
     2761 gtttttgagt ttcaaaagcg gttttatgaa attttttccg cgctcagaga aaaaaaggtt
     2821 cttcctcttc tggtcggggg cacaggactg tacctcgaat ccgtcctgac ccgctacgtt
     2881 ctgccggagg ccgaaccgga cgagcggatt cgggaagagc tggcggggct gacgatggga
     2941 gagcttcagg agatgctgct ggccatgaag ccccaactgc acaatcaaac ggatcttcac
     3001 gaccgggaaa ggctggtccg aaaaattgaa atcgagaaag ccagacaaaa cccgtcaaag
     3061 agtcaaaccc ggtttccgca gattcaagcc ggcatcttcg gcattttctg ggagcgggcc
     3121 gaactgagga agagaatatc ggccaggctt cacataaggc tggcggaggg gatgatcgaa
     3181 gaagtgtccc gcctgcacga tgaaggtgtg tcctgggaga ggctggagag ctttggtctg
     3241 gaataccggt tcattgcccg gtatctgcag aagacgatga cccgggacga gatggcggcc
     3301 gggctgcaga ccgccatcgg acagtttgcc aagcgtcaga tgacgtggtt tcggcggatg
     3361 gaaaaaaagg gcgtcgcgat tgagtggatt cccggcggcg attatccggc gctcgcgagc
     3421 cgcgttatga aaatgttgtc ctgaagcgca tacagccccg gcctttacgc aatgatccca
     3481 tcaaaccgga aagtcgtttt cccgtgaaaa aagattcctt gttcgaaaat tatctgaacc
     3541 ggtacgcggc agggttgccc tggcgtctgc aggcggcgga ttcacgcgct tgtgaacaga
     3601 ttgtcgtgat tccggcgtat gcggaaaggg actcgctttt ttgcacgctg gcgtccgttg
     3661 ccgccaatcc tgaggcgatg ctggtaaaaa cgatggttct gtgcgtggtc aacaacaggg
     3721 ctgcctcgcc cgctgaagat aaggaaaaca acgcgcagac gctggcgatg ttaaatgcgc
     3781 tggtcaggcg gcagtctttt gacattccga aagcggacgg aaaactccgg gaagcatggc
     3841 aggccgttgc cgcaagtccg ctcaggctgg ggtttatcga tgcgacttcc cccggactgg
     3901 aaattcccga ccgcgcagga ggcgtaggga tggcccggaa aatcggcatg gacgcggcgc
     3961 tgcgcctgct ggcaacgtcc gaacagaaac cccgtctgat tttgagcctc gacgcggaca
     4021 cgctcgttcg gccggattat ctgtcgggtg tccgaagcgc gtttgcttcc ggccgggcgc
     4081 aggccggcat actggactac gaacatcaaa tgcccccgga cctggccggg cagacggcca
     4141 tttgccgcta cgaaatcttc ctgcgctact gggttctggg gcttcaattt gcctgctccc
     4201 cctatgcgtt tcattccatc ggctccacga tggcaaccac ggacgaggga tacctctccg
     4261 tgaggggcat gaatcgccgc gaggcgggcg aggattttta ttttctgaac aaactggcca
     4321 aagccggccc cctgcgcaga attcgtgaaa cgcgcgtcta tccttccggg cgggcttccg
     4381 cgcgggtccc cttcgggacg ggcgccgcgg tggccaaaat ggtttccggc gcgcggagcg
     4441 atttcctcct gtatgatccg cgaatattta ttgtgctgag atcatggatc gcgctgatga
     4501 aagaatcctt tgaagacacg gcggatgaaa tactcggaaa ggcgaaagag attcatccgg
     4561 ggctttcgga atttctggcc gcgcggggat ttctgacgat ccaacccaga atacgcgcca
     4621 acgtaaagga ccgcaggacg tatgaaaggc agatgcacgg ctggtttgac ggttttgaaa
     4681 ccttgaagct gatcaacggc ctgaccaggg atttctatcc aaaaattctc ctgcctgccg
     4741 cggtgacgca gattctggag atgcaaaacc tggaatgtcc acgccggttc tccggtgaaa
     4801 gcgcgcccgg ctccgaagac tgcatggaga tgctgtctta ccttcgaggc ctgacctgaa
     4861 tgcggaaaat caacaacgtc ggaatctcct tgactttgca aaagaacttc gcgtataggc
     4921 catccaatga tgaactcgta aaaagtcatt caagttgtca ccccggcgaa ggccggggtc
     4981 cgtaactcat tgcaattact ggataccggc cttcgccggt atgacgtaaa gggtgcgaaa
     5041 atgacttttt acgagaccgt aaaacaataa tttctgaaaa gggttgtgga ggaaattcat
     5101 gaataaattc tttttcaaaa gcgctttggc gattttcatg ctggcgctgg ttgtctcggc
     5161 gtttgcctgc aacaaaaaag aggaggccgc aagtaagagc gagcctgccg cttcgcaaag
     5221 caccgcttcc gaatcgatcc tgataccgga caaaccggcc gcggaaatgc cggcgcctgc
     5281 tattgctccc gctcagccgc ttccgtccga cgcggcagtc agtgtggacg gtgttgccct
     5341 gaaaaaggtc gcactggaaa aaagagtgca ggcgcagatg aagctttatc aggacaagat
     5401 tccggccgac cggaaaaatg aagtccgggc cggtttgaaa aaacagttga cggaagattt
     5461 tgtgatgcgg acgcttctgg ccaatgaagt caagcgcaaa aacatttcgg ccacggacaa
     5521 ggaaatctcg gcggccatgg aacagatcaa ggccagtatt ccgcccgata aaaaactcaa
     5581 cgaatttttg aaggaaaaca acgtatcccg cgatgacatt gcgctgggcg tccgcatcca
     5641 aaagctggtc gccatggaaa ccggcggaaa aatcaagccg acggacaagg aaatcggcaa
     5701 gttttacacg gacaataaaa ataagtttac caccgaggaa agcgttcatg tccgtcatat
     5761 tctggtcgcc atggatgcca aggacgacga gaaggtcaag gctgaaaaaa aggcgaagat
     5821 agaaggtttg cgcaaacaga ttatgggcgg agctgatttt gccgaggtgg ccaaaaacaa
     5881 ttccgactgc cccagcaagg aaaacggcgg ggatctgggc gaaatcaaga aaggccagac
     5941 cgtcaaaccc tttgaggacg cggcgttttc acaggagatc aaagcggtcg gaccggtggt
     6001 gagcacggaa ttcggccatc acattattca ggtgctgggg cgcacgccgg cgcataccgt
     6061 cagcctgggc gaagtgaaag agcagatttc cctgtatctg gagcagcaga aacaggcgga
     6121 agccttttcc aaaatgatgg cgcggctgaa aaagaacgcg gttattcagt acggcaaccc
     6181 ttaacactgt cgtaaaaagt caatttcggc gccttttcgt cataccggcg aaagccggta
     6241 tccagtaagt acagtgggtt atggaccccg gccttcgccg gggtgacggc tcgaattact
     6301 ttttacgggt tcatcaatct tgatgaaccc gtaaaaaatc accactgtca ccctgagccc
     6361 gtcgaagggt gaacgtaagt actctatatc acgtcatgct tcgacaggct cagcatgaca
     6421 aaagagggac aagatgactt tttacgaact tgtcaacctt gaatcatgag aatcaggcag
     6481 gcgggtcgaa tcgatgacgg tctctggctt ctgggcacgc aggaatcctg cgtgtatctg
     6541 ctggaaggaa ccaaaacctc ggtcctgatc agcgccggca tgccttacat tcttccggat
     6601 cttttccgtc agatggcttc gcggggaatg acgccggaga aaatcgaaca ggttgtcatt
     6661 ctgcacgctc attttgatca tgtcggcgtg gttccttatc tgaaaagaaa ccggccggac
     6721 atgaagattt atgcgtccgc gcgcggttgg gaggtgctga caaatcccag agccgtttcc
     6781 gtcatcaacg aattttcgga aaaagtccgc cgaaggatga agggaagcga agattctctg
     6841 gcggggctgg actgggcctg gcgtgatgac gtgaaaggag aaacgctggc gcagggagac
     6901 cgcctggacc ttggcggccg gcagttgcag tttttcgaaa cgcccgggca ttcttcctgc
     6961 tcgatttccg cctgggttcc cgggctgaac attctttttc cctcggatgc cgtggccatt
     7021 ccctaccggg acgaatatat catcgccgtc ggctcgagct ttgaaaagta cctggccagc
     7081 ctggataagc ttgccggtct ggacgtcagg aaaatttgcg cggaccatta cggatgcgtt
     7141 acggaagaag aggcgagggg atacattgcg caaaccagga ccgcgacgct tcagatgatg
     7201 gaccggatgc gcgaggcgct ggcgcgggag aaaagcgtcg aggaggcggc ccggcaactg
     7261 atccggcttc actatgaact ccggccggac tactttgtcc acccggacat tctgcttcga
     7321 acctacacgc agatgctcaa gcaactcacc ggctaaggtt tttacttgaa gctgtggtct
     7381 gcgtccggga aggcgccggt tttcacctct gaggcgtagc gcttggcggc gtttttgatt
     7441 tcctgactca aatgagcgta ttttttgaca aatttcggcg tcatccggtc gttcatgccc
     7501 agcatgtcgt tcaccaccag aacctgtccg tcgcaggaaa ccccggcccc gatgccgatg
     7561 gtcggaatgg tcagggtgct cgtaatttcc gcggccagtt tttccggcac acattccagc
     7621 accacggaaa aggcgcctgc ctcttcgagg atcaaggcgt ccctcatcat tttttccgcc
     7681 gcatcctctt tcttgccctg cactttgtag ccgccgagct ggtgaatgga ttgcggcgtc
     7741 agccccagat gcgccatgac cggaatgccg gagagcgtca ttttgcggac aatttccgcg
     7801 tgctccccgc ctccttcaag cttgacggcc tgagcgttgc cttcttttaa cagtcgtcct
     7861 gcgttggcca tcgccgcttc ctgggacacc tggtaggaaa gaaagggcat gtccgccaca
     7921 atcagcgctt tctgcgaccc ccgggccacc gcccgcgtgt ggtgaatcat gtcttccatc
     7981 gtcacgggga gcgtggtgtc gtagcccaga acgacattgc ccagagaatc gccgacgaga
     8041 aggatgtcga tgtcgctttc gtccaggatg gacgccatgc cgaagtcgta ggccgtcagc
     8101 atggcgattt tttcaccctg cgttttcatc ttcttgatgt ccagaatggt ttttcggttg
     8161 atctttgtct gcgtgctcat gattttgctc ctttcagcat ccccaggatt tctttggcct
     8221 gagcggcgct caaggttcct ttttcccggg ccagtttcac cgcgacggcg cccatgtgcg
     8281 catacatcga agcgaagtcg ggaagatggc gggccatggc gtcgatatgt ttttgaaccg
     8341 ttcccgaatc accgcgcgcg atcggaccgg tgagggcatt cgggcagccc tgattttcaa
     8401 tatttttcaa actgccgtac accagcggca gataagcccg gcgggcgtct ttttctgaaa
     8461 agccgatgga catgtaaatg gattcgacaa cgctcaagag cgaaaccagg taattcgagg
     8521 cgatgcaggc ggccgcgtgg tacaggggtt tttgcgcggc ggagatggga atggggatgc
     8581 cctgaaggtc gcgtacgatg tccgcggcga gtcttttcac gcccgcatcg gccgtgacgc
     8641 cgaagtagct gcccggaatg ctggcaatgg ctccgtcaat ggaggaaaag ctttgcagcg
     8701 gatggatgct ggcggtcagt gcgccggcgc gtgccgcggg ggccagcagg tccagaccgc
     8761 ccgcgccgct catgtgaaaa acgttcttcc cttttaaacc cgtccgggcg gcaatctccg
     8821 cgcatacgga agcgatgcgg tcgtccggcg tcgtaatcag cacacagtcg gcgagggcgg
     8881 ccgcttgtgc cggatgggga aaaccaggcg cccgaaggta ggaggcagcc cgttttcgat
     8941 gggcggcgga gcaatcggca acggccgcaa catggcggcc ggccttgccc agcagaaatc
     9001 cgatggccgt tccgaccatg ccggccccga caacggcgaa ggtgagcggc ggctttttcc
     9061 tcttcatttt cacgtcccaa aaaaaagggc cttccgcagc gtaaggaaga cccgtatttg
     9121 acagttttgt attgactccc gtctcagtct gcaacaagtg atccaagcgg ttggacgctt
     9181 cctaa
//
LOCUS       DAOSRM010000087         9108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_7267_length_9108_cov_108.389153, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000087 DAOSRM010000000
VERSION     DAOSRM010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7267_length_9108_cov_108.389153"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLA58_08340"
     CDS             <1..244
                     /locus_tag="PLA58_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR62840.1"
                     /translation="AMFMGGWCCNPWPLLEKINCPALVIEGEQSPNVAFVDLRRAVSL
                     LPRGEFGSVAGAGHLIPMEKPGEITAIIRDFIAKLP"
     gene            296..1063
                     /locus_tag="PLA58_08345"
     CDS             296..1063
                     /locus_tag="PLA58_08345"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOR62841.1"
                     /translation="MAGPDIIAFCNQKGGVGKTTCALNIAVGLTLLRKKVLVIDFDPQ
                     AHLTYSLGLDAHHLTGTVYSVMKGTAPISQAAMPIRGMSVLPATLELARLETEMAGLP
                     EKESLLKKRLEDVSDAQYVIIDCPPASGLLTVNALVACREVFIPLQMEFLALKGMSRL
                     LTLIEDVKKRYNHNAPTYRVIPTRYDARKRLNNAIMDKVRERFGDRVFKAVIRENIAV
                     AEAPSFGLSIFEYAPKSHGAEDYLDLCREILRKRPAG"
     gene            1140..1889
                     /gene="fabG"
                     /locus_tag="PLA58_08350"
     CDS             1140..1889
                     /gene="fabG"
                     /locus_tag="PLA58_08350"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF004198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOR62842.1"
                     /translation="MRLKDKVAIVTGSGRGIGEGIVMRFAEEGAKIIVNDVNEADAKA
                     VVEKIRAKGGQALAVLGSVADRAVVQKMVDTAVAEFGTLDIIVNNAGITRDVMLHKMT
                     DDQWKAVIDVNLTGVYYGIQCAGRVMREKKYGKIINISSTSALGNAGQLNYSASKAGV
                     IGMTKTAAKELGPKNVNVNAIAPGMIWTDMMKGMPPDVLQQMDMMLPFIVPLNRKGSP
                     QDIANLALFLASDESAFITGQIIFCDGGMKM"
     gene            1975..2736
                     /locus_tag="PLA58_08355"
     CDS             1975..2736
                     /locus_tag="PLA58_08355"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOR62843.1"
                     /translation="MKPVFNFSGKTAIVTGAGRGIGRVIALGLAQAGANVVLCSRTKA
                     EIDAVAGEISGNGGKALAVATDLTVHGQLENLVDAAIKEFGRIDILVNNAARSFLRGL
                     LDLREDGWDKCFNTNVKAVWLLSRLVARKMMEQKSGKIINITTVGAEKAEVGMAAYGC
                     SKAALKHLTRCMAREWAQFGINVNAVAPGFTRTDFSRPIWSNPDVEKLICQAIPKGKI
                     GEPEDVVGAVLFLASGAADYITGETIYVDGGTMTA"
     gene            2758..4731
                     /locus_tag="PLA58_08360"
     CDS             2758..4731
                     /locus_tag="PLA58_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HOR62844.1"
                     /translation="MSNQGVVSPVVADIRSGMKRGKVVSLEDAMRVIRDGDTIAVNGF
                     VGYSPEELLQGLEDRFLKTGEPKNLTIIVAAGIGDGKEKGVNRLAHDGLVRRVIAGHW
                     GLIPKMQKLALAGKIEAYNFPQGVITHMYRDIAGHLPRTITHVGLGTFVDPRLSAGKV
                     NAITTEDLVERITFDGKEYLAYKTMPVSVVFLRGTTADMDGNITMEKEPLTLEMLPMA
                     MAAKNSGGFVIVQVERIADRHALNPRHVKVPGILVDCVVVAKPENHRQTYSTYYNPAY
                     SGEFKVPTATVEPLPMDERKLVARRAAFELRPNMVVNLGIGMPEGVANVANEEGIFDY
                     LTLTAEAGSIGGIPSGGGDFGTATNMDFLNDMHAQFDYYDGGGLDLACLGLAQMDSHG
                     NVNVSKFGPKLAGCGGFINITQNAKKIVFAGTFTAGGTQLAVENGKLKIVQEGSLKKI
                     VREVEQITFSGKTAQQGEQQVFYVTERCVFRLTREGVELIEIAPGIDLEKDLLAQMEF
                     KPIMKNVRPMDERIFKLPPMGLKDDLLSIPIPDRLTYDPATNIFYVNFEGLNVRSSAD
                     IEAIRSRVTKVCAPLGKRVKTIVNYDNFSIAPDLEDEYVKMVKFVVSEYYSDVTRYTT
                     SAFLRMKLGDELKKRNLAPHIFQSKEEAREALE"
     gene            4881..5591
                     /locus_tag="PLA58_08365"
     CDS             4881..5591
                     /locus_tag="PLA58_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOR62845.1"
                     /translation="MSIDVKLEGHVAVVTLNEGENRLNQDFLQKFIDTLDQVEKETQA
                     TVLVVRGADAKIFSNGIDLEWLMPIMQKNDTATAKKFIETMMALFRKIALYPLTTIAA
                     MTGHAFAGGAIMCCYFDFRFMRSDRGFMCFPEVDLGIPFLPGMMMAMKKAIPRYKLDE
                     MVMTGKRCAAQECEEHHIITKACHIDQLMDEVMKFANLQNKRRPVVELIKAEMNKAIV
                     YAIDHEDPPIIASGRFYV"
     gene            complement(5655..6953)
                     /gene="hisD"
                     /locus_tag="PLA58_08370"
     CDS             complement(5655..6953)
                     /gene="hisD"
                     /locus_tag="PLA58_08370"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOR62846.1"
                     /translation="MKILRADAKAFHHFFAALRTRGGAFSPKLLSDVARIVREVSVRG
                     DEALFSYTRKFDGHDLTPATVEATDAEKEEALAGVNPQDREVIRLAAARIEKYHRRQI
                     AQGWSMKDEDGAELGQRILPLARVGVYAPGGKAFYPSTILMAAIPARLAGVKEVLLAS
                     PVKDGRLSPLIAAAAEAARVDRIFKIGGAQAIAALAYGTESVPRVDKIVGPGNAYVAA
                     AKKLVFGQVAIDMIAGPSEVAIIADSTADPVFAAADMLAQAEHDEKAAAVLFTPDPDL
                     AREVAAEIRRQIKPLPRKKIIQKSLSSFGAIIITADIDEAAALTNLFAPEHLELMVEN
                     PTNALRHIRSAGSVFLGSYTPEALGDYIAGANHILPTEGTARFSSPLGVYDFYKRMSV
                     LSFSRAAFENLSEATRHFARMEGLCAHANSVQVRCKSGKN"
     gene            complement(6955..8202)
                     /gene="murA"
                     /locus_tag="PLA58_08375"
     CDS             complement(6955..8202)
                     /gene="murA"
                     /locus_tag="PLA58_08375"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOR62847.1"
                     /translation="MDKIVISGGRPLHGDVQISGAKNAALPVLTAALLTEEPCTFSNI
                     PDLVDIKTAGKLLRNMGVEIEGAGTVKISAEKLTNSVAPYDLVKTMRASILVLGPLVA
                     RTGKARVSLPGGCAIGARPVNLHIKALQDMGATVDLQGGYIEARAGRLKGADIYFDLP
                     TVTGTENIMMAATLAEGTTVLNNAAREPEIVNLADVLRGMGAKINGAGTDVITITGVS
                     RLSGTEAAIIPDRIEAGTFMIAAGITGGEINVLGCVPQHLEALINKLRDTGMKIQPIE
                     GGLRVAAGNKIASVDVKTLPHPGFPTDLQAQIMAYLCVGSGLSVITETVFENRFMHVS
                     ELMRMGADITIQGGSAVVRGVPALNGAQTMATDLRASASLILAALVARGETEISRVYH
                     IDRGYENIEKKFSALGADIKRVK"
     gene            complement(8203..9054)
                     /gene="prmC"
                     /locus_tag="PLA58_08380"
     CDS             complement(8203..9054)
                     /gene="prmC"
                     /locus_tag="PLA58_08380"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOR62848.1"
                     /translation="MTIRQIIFEATQKLEKAGIPSARLDAEVLLAFLLRCDRLAFFKN
                     PENPISKEQRAAFDRLIERRTGGEPVAYMTGRKAFWSFALEVNPAVLIPRPDTEVIVE
                     EALAVLRQESWRRPRILDIGTGSGAIALALACEVPGASITATDISEAALQTAQKNAAA
                     LNVEPIVFLKGDLFEPVEGRFDLIVSNPPYIGADEYQSLPPGVKNFEPEEALRAGQTG
                     LEFYEKLIYQAHGYLAENGWLLLEIGARQKQEVRAIYEAHQDFYERIDVREDYAGLPR
                     VIKGRRR"
BASE COUNT         1981 a         2599 c         2714 g         1814 t
ORIGIN      
        1 ggccatgttc atgggcggct ggtgctgcaa tccctggccg cttctggaaa agatcaactg
       61 tcccgcgctg gtgatcgagg gggagcaaag ccccaatgtc gctttcgtgg atctgcgccg
      121 ggccgtctcg ctcctgccgc gaggggagtt cggatccgtg gcgggtgccg gtcatctgat
      181 ccccatggaa aaacccggcg agatcaccgc catcatcagg gactttatcg cgaagctgcc
      241 ctgaacgtcg cgcgtacggt cgcctgctgc cggaaaaaca ctggagacac aaaacatggc
      301 cggtcccgac atcatcgctt tttgcaacca gaagggaggc gtgggaaaga caacctgcgc
      361 cctcaacatc gccgtggggc tcacgctgct tcggaaaaaa gtgctcgtta tcgatttcga
      421 tccgcaggcg cacctgacgt actcgctggg cctcgacgcg caccatctca caggcacggt
      481 ttattccgtc atgaagggga cggcgccgat ttcccaggcg gccatgccca tcaggggcat
      541 gagcgttttg ccggctaccc ttgagctggc gcgcctggaa acggaaatgg ccggacttcc
      601 cgaaaaggaa agccttctga aaaaacgcct cgaggatgtg tccgatgcgc agtacgtcat
      661 catcgactgc cccccggcct ccggcctgct gacggtcaac gccctggtgg cctgtcggga
      721 ggtttttatc ccgctccaga tggaatttct ggcgctcaag ggaatgagca ggcttctgac
      781 gctgatcgaa gacgttaaaa agcgatacaa ccacaatgcg ccgacgtacc gggttattcc
      841 gacccgatat gacgcgagaa agaggctcaa caacgccatc atggacaagg tgcgcgagcg
      901 cttcggcgac cgcgttttca aggccgtcat ccgcgaaaac atcgctgtgg cggaagcgcc
      961 cagtttcggc ctgtccattt ttgaatacgc tcccaaaagc catggcgcgg aggactatct
     1021 ggacctttgc cgggaaattc tccgcaagcg gccggccggc tgagaggatc gtgatgcgaa
     1081 gcgacggaaa aaagccgata aacggttgac ccccgaaaaa aaattgaaaa ggagatcgta
     1141 tgagactgaa agacaaagta gccattgtga cgggatcggg acgcggcatc ggagaaggca
     1201 ttgtgatgcg ctttgccgaa gaaggcgcga aaattatcgt aaatgacgtc aacgaagcgg
     1261 atgccaaggc cgtggtggaa aagatcaggg ccaaaggcgg gcaggctctc gccgttctgg
     1321 gcagcgttgc cgaccgggcg gtggtccaga aaatggtcga tacggccgtg gccgaattcg
     1381 gcacgctcga cattatcgtc aacaatgccg gcatcacccg cgacgtgatg ctgcataaaa
     1441 tgacggacga ccaatggaaa gccgtcatcg acgtgaatct gacgggcgtg tactacggca
     1501 ttcagtgcgc ggggcgcgtc atgcgcgaga agaaatacgg aaagatcatc aacatttcct
     1561 cgaccagcgc gctgggcaac gcaggccagc tcaactactc ggcctcgaag gcgggcgtca
     1621 tcggcatgac caaaaccgcg gccaaggagc tggggccgaa aaacgtaaac gtcaacgcca
     1681 tcgcgccggg catgatctgg acggacatga tgaaaggcat gcccccggac gtccttcagc
     1741 agatggacat gatgctgccg ttcatcgttc cgctgaaccg gaagggatcg ccgcaggata
     1801 tcgccaatct ggcgctgttc ctggcatccg atgaaagcgc gtttatcacc gggcagatca
     1861 ttttctgcga cggcggaatg aaaatgtaaa aaaaggcgat cggacgcgag gcgctggttt
     1921 tcaagtcgtc cgcggcggtt ttccgtatga cataaatgta aggaggagaa acgcatgaaa
     1981 cctgttttca acttttccgg aaagacggcg attgtgaccg gagcgggccg cggcattggc
     2041 cgcgtgattg ccctgggcct ggctcaggcc ggcgcaaatg tggtgctgtg cagcagaacg
     2101 aaagcggaga ttgacgccgt cgccggtgaa atctccggca acggcggcaa agctctggcg
     2161 gtggcaaccg atctgacggt tcatggacaa ctggagaatc tggtcgatgc ggcgattaaa
     2221 gaattcggcc ggatcgacat tctggtgaac aacgccgccc gcagttttct gcgcggcctg
     2281 ctcgatcttc gcgaggacgg ctgggacaag tgtttcaaca ccaatgtgaa ggccgtctgg
     2341 cttttaagcc gcctggtcgc gcgaaaaatg atggagcaaa aaagcgggaa aatcatcaac
     2401 attaccacgg tcggcgcgga aaaagccgag gtgggcatgg cggcttacgg ctgcagcaag
     2461 gcggcgctca agcatctgac ccgctgcatg gcgcgggagt gggcgcagtt cggcatcaat
     2521 gtgaacgccg tcgcccccgg atttacccgt acggatttca gccggccgat ctggtccaat
     2581 cccgatgttg aaaagttgat ttgccaggcc attcccaagg ggaagatcgg cgaaccggaa
     2641 gacgttgtcg gcgcggtgct ttttctggct tccggcgccg cggattatat caccggagag
     2701 accatctacg tggatggggg aaccatgacc gcgtagcaat cacacgggag gtgttccatg
     2761 agcaaccagg gcgtcgtcag tccggtcgtt gcggatatcc gctccggcat gaagagaggc
     2821 aaagtggtgt ccctggagga tgccatgcgg gtgattcggg acggagacac catagccgtc
     2881 aacggctttg tcggttactc ccccgaagag ctgctgcagg gtctggaaga ccgctttctg
     2941 aaaacgggcg aaccgaaaaa ccttacgatt atcgtcgcgg ccggcatcgg cgacggcaag
     3001 gaaaagggcg tcaaccggct ggcccatgac gggctggtcc ggcgcgtcat tgccgggcac
     3061 tggggtttga tccccaaaat gcagaagctc gccctggcgg ggaaaattga agcctacaac
     3121 tttccccagg gcgtgatcac gcatatgtac cgcgatatcg cgggacacct tccccggacg
     3181 atcacccacg tgggattggg aactttcgtt gatccccggc tgtccgccgg caaggtcaac
     3241 gccatcacca cggaagacct ggttgaacgg atcaccttcg acggcaagga gtatctggcg
     3301 tacaagacga tgccggtttc ggtcgtgttt ctgcgcggca ccacggcgga catggacggc
     3361 aacattacga tggaaaaaga gccgctgacg ctggaaatgc tgccgatggc catggcggcc
     3421 aagaactcgg gcggattcgt catcgtgcag gtcgagagaa tcgccgaccg ccacgcgctc
     3481 aatccccgcc acgtcaaggt gcccgggatt ctggtggact gcgttgtcgt ggcgaagccg
     3541 gaaaatcacc ggcagaccta ttccacatac tataatccgg cctacagcgg cgaattcaag
     3601 gttcccacgg ccaccgtgga gccgctgccc atggacgaac gaaagctcgt cgcgcgccgc
     3661 gccgcctttg agcttcggcc gaacatggtg gtgaatctgg gcatcggcat gcccgaaggc
     3721 gtagccaatg tcgccaacga ggaaggcatt ttcgattatc tgacgctcac ggcggaagcg
     3781 ggctcgatcg gcggcattcc ctccggcggc ggggatttcg gcaccgccac caacatggat
     3841 tttctcaacg acatgcatgc ccagtttgat tattatgacg gcggcggact ggatctggcc
     3901 tgtctggggc tggcccagat ggattcccac ggcaacgtga acgtcagcaa attcggcccc
     3961 aagctggccg gctgcggcgg cttcatcaac atcacgcaaa acgccaaaaa aatcgttttc
     4021 gccggaacgt ttaccgccgg cgggacccaa ctggccgtgg aaaacggcaa gctgaaaatc
     4081 gttcaggagg ggagcctgaa aaagattgtc cgggaagtgg agcagatcac cttcagcggc
     4141 aaaaccgccc agcagggcga acagcaggtg ttttacgtca ccgaaaggtg cgtcttccgg
     4201 ctgacgcgcg aaggcgtgga gctcatcgaa atcgcgcccg gcatcgacct ggaaaaagac
     4261 cttctggcgc agatggaatt caaacccatt atgaagaatg ttcgcccgat ggacgagcgg
     4321 atcttcaaac ttccgccgat ggggctcaag gatgatctcc tgagcattcc gatccccgat
     4381 cgcctcacgt acgatccggc gaccaacatc ttttatgtta attttgaggg cctcaatgtc
     4441 cgcagctcgg ccgatatcga ggcgatccgc tcccgcgtga cgaaagtgtg cgccccgctg
     4501 gggaagcgcg tcaaaacgat cgtcaactac gacaactttt ccatcgcgcc ggatctggaa
     4561 gacgaatacg tcaagatggt caagtttgtg gtgtccgagt actacagcga cgtcacccgc
     4621 tataccacga gcgcgtttct gcgcatgaaa ctgggcgacg agctgaaaaa acggaatctg
     4681 gcgccgcata ttttccaatc gaaggaagag gcccgggagg cgctggaatg aaaaaggctg
     4741 cggggctgag ggtaaagcaa gaacggaaat gggggttgaa aatatttccg caaaaagata
     4801 agagtcatcg ggcgtaaaga gaaaaggccg tacaaacggt caggaaccgt taaaaaaata
     4861 ttgaggaaag aagaagaatt atgtcgattg atgtcaagct ggaaggccat gtggccgttg
     4921 tgacgctgaa cgaaggggaa aaccggttga atcaggattt cctgcagaaa tttattgaca
     4981 ccctggatca ggtggaaaaa gagacccagg ctaccgtgct ggtggttcgg ggagcggatg
     5041 ccaaaatctt cagcaacggc atcgacctgg aatggctgat gccgatcatg cagaaaaacg
     5101 acacggccac cgccaagaaa tttatcgaaa cgatgatggc gctgttccgg aagatcgccc
     5161 tctatccgct gaccaccatc gccgcgatga ccgggcacgc ctttgccggc ggcgccatca
     5221 tgtgctgcta ttttgatttt cgcttcatgc ggtcggatcg gggcttcatg tgctttcccg
     5281 aagtcgattt gggcattccg tttttgccgg gcatgatgat ggcgatgaaa aaagccattc
     5341 cccgctacaa gctcgacgag atggtcatga ccggaaagcg ctgcgccgcg caggaatgcg
     5401 aggagcacca catcatcacc aaggcctgcc acatcgatca gttaatggac gaagtcatga
     5461 aatttgccaa ccttcagaac aaaagacgtc cggtcgttga actgatcaag gcggaaatga
     5521 acaaggctat cgtttacgcg atcgatcatg aggacccgcc gatcatcgcg tccggtcgct
     5581 tctacgtttg atcagatagg cagtttgtta tcaaccattt tcagggcagg gaacggtcgc
     5641 gaccgttccc tgtattaatt tttaccgctt ttacatcgga cctgcaccga attggcgtgc
     5701 gcgcagaggc cttccattcg cgcgaaatgc ctcgtcgcct ccgacagatt ttcaaacgcc
     5761 gcccgggaaa aagacaggac gctcatccgc ttgtagaaat cataaacccc cagaggcgaa
     5821 gagaagcgcg ccgtgccttc cgtgggtaga atgtggttgg cgcccgcaat gtaatcgccg
     5881 agggcttccg gcgtataaga ccccagaaaa acggaacccg cgctgcgaat gtgcctcagg
     5941 gcatttgtcg gattttccac catcaattcc agatgctccg gcgcgaagag attggtcagc
     6001 gccgccgctt catcgatatc ggccgtgatg atgatcgcgc cgaaggaaga aagggatttt
     6061 tgaatgattt ttttccgggg caggggtttt atctgccgcc ggatttctgc ggcgacctcc
     6121 cgcgccagat cgggatcggg cgtgaaaaga acagccgctg ccttttcgtc gtgttcggcc
     6181 tgcgccagca tgtccgccgc cgcgaaaacg gggtcggcgg ttgaatcggc gatgatcgcc
     6241 acctcgctcg ggccggcgat catgtcgatg gccacctgtc cgaacaccag ctttttggcc
     6301 gcggcgacat aggcgttgcc cggccctacg attttgtcca cgcgcggaac gctttcagtt
     6361 ccgtatgcca gagcggcaat ggcctgcgcg ccgccgatct tgaaaatgcg gtccacccgg
     6421 gcggcttcgg ccgccgcggc gatgagcggg gaaagccggc cgtccttcac ggggctcgcc
     6481 agcagaactt ccttgacccc cgcgagacga gcggggatcg ccgccatcag gatggtggaa
     6541 ggataaaagg cctttccgcc cggcgcgtaa acgcccaccc gcgccagcgg cagaatccgc
     6601 tggcccagtt cggcgccgtc ttcgtctttc atggaccatc cctgcgcgat ctgccggcgg
     6661 tgatactttt caatccgcgc cgccgcaagc cggatgactt cccggtcctg cggatttacg
     6721 ccggccagcg cctcttcttt ttccgcgtcc gttgcttcga cggttgcggg cgtcaggtcg
     6781 tggccgtcga atttccgggt gtaggaaaac agggcctcgt ccccccgaac ggacacttcc
     6841 cgaacgatcc gggccacgtc cgacagcagc ttcggtgaaa atgccccgcc cctggttctc
     6901 agggcggcaa aaaaatggtg gaaggctttt gcgtcggctc gaagaatttt catcttattt
     6961 tacccttttg atgtccgcgc ccagcgcgga aaatttcttc tcgatattct cgtaaccccg
     7021 gtcaatgtga tagaccctcg aaatctcggt ttcaccccgg gcgaccagcg ccgccagaat
     7081 cagcgaggcg gaggcccgca gatccgtggc catcgtctgc gcgccgttca aagcgggaac
     7141 gccgcgcacc acggcgctgc cgccctgaat cgtgatgtcc gcgcccatgc gcatcagttc
     7201 gctcacgtgc atgaagcggt tttcaaagac cgtttccgtg atgacggaaa ggccgctgcc
     7261 gacgcacagg taggccatga tctgcgcctg cagatccgtc gggaagccgg ggtggggaag
     7321 ggttttgacg tccacactgg caattttatt tccggcggcc acgcgcagac ccccttcgat
     7381 gggctggatt ttcatgccgg tgtcccgcag tttgttgatc agcgcctcca ggtgctgggg
     7441 aacgcatccc agcacgttga tttccccgcc ggtgattccg gcggcgatca tgaaggtgcc
     7501 ggcttcaatc cggtcgggaa tgatagccgc ctcggttccg gacagtctgg acacgccggt
     7561 gatggtgatc acgtccgttc ccgcgccgtt gatttttgcg cccatgcccc ggagcacgtc
     7621 cgccagattg acgatttccg gctcccgcgc ggcgttgttg agcacggtgg tgccctccgc
     7681 cagggttgcc gccatcatga tattttccgt gccggtcacc gtgggcagat caaaataaat
     7741 atcggcgccc ttcagtctgc cggccctggc ttcgatatag cctccctgca gatccacggt
     7801 cgcgcccatg tcctgaagcg ccttgatatg cagattgacg gggcgcgctc cgatggcgca
     7861 gccgccggga agagagacgc gggctttgcc cgtgcgggcc accagcggcc cgagaaccag
     7921 gatggaagcg cgcatcgtct tgaccagatc atagggggcg acgctgttgg tcaatttttc
     7981 agcgctgatt ttgaccgtgc ccgcgccttc gatttccacg cccatatttc ttaagagctt
     8041 gccggcggtt ttaatatcga ccagatcggg aatattggaa aacgtgcagg gctcttcggt
     8101 cagaagcgcg gccgtcagca ccggcagggc ggcgttcttc gcgccgctga tttgaacgtc
     8161 tccgtgaagc ggcctgccgc cggaaataac aatcttgtcc acttaccttc tccttccttt
     8221 gatgacgcgc ggcaatcccg cgtagtcttc acgcacatcg atgcgttcgt aaaaatcctg
     8281 atgggcttca tagatggcgc gaacttcctg tttttgtcta gcgccgattt cgagcaaaag
     8341 ccagccattt tctgcaaggt atccgtgtgc ctgatatatc aacttttcgt aaaattccag
     8401 acctgtttga cccgcgcgca gcgcttcctc cggctcgaaa ttttttacgc ccgggggcag
     8461 ggattggtat tcatccgccc cgatgtaggg ggggttggac acaatcaggt caaaccggcc
     8521 ttcaaccggc tcgaagagat cgcccttgag aaacacgatt ggttccacgt tcagtgccgc
     8581 ggcatttttt tgcgctgttt ggagggccgc ctccgagata tccgtggcgg tgatggacgc
     8641 gccgggaact tcgcaggcca gcgccagcgc gattgccccg cttcccgtgc cgatatccag
     8701 aatgcgcggg cggcgccagg actcctgccg caagacggcc agagcctctt cgacaatcac
     8761 ttccgtgtcg gggcggggaa tcagcacggc gggattgacc tccagggcaa aggaccagaa
     8821 cgctttccgg cccgtcatgt aggccaccgg ctctccaccc gttcttcgct cgatcagccg
     8881 gtcaaaggcg gcccgctgct ctttgctgat gggattttcc gggtttttga aaaaagccag
     8941 acggtcgcat cgcagaagaa atgccagcag aacttccgcg tcaaggcggg ccgaaggaat
     9001 ccctgccttt tcaagctttt gcgttgcttc aaaaatgatt tgccggatcg tcatttttcc
     9061 cctttgtaac gaggagttcc gtatagccac tccgaaaatt gattaatc
//
LOCUS       DAOSRM010000088         8837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_7552_length_8837_cov_109.964814, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000088 DAOSRM010000000
VERSION     DAOSRM010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7552_length_8837_cov_109.964814"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /gene="carB"
                     /locus_tag="PLA58_08385"
     CDS             <1..659
                     /gene="carB"
                     /locus_tag="PLA58_08385"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418947.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOR62849.1"
                     /translation="AIGVPLAKLATRVMLGKTLGELSFTETIIPKHVCVKEAVFPFNR
                     FPGVDIILGPEMKSTGEVMGIDHSFGLAFAKAQMATGFKMPTAGSVFISVHDCHKAGI
                     VDVARTFADLGFRILATAGTAGYLNRQGIIAAAVNKVSEGRPHVVDLIKNGDIQMVIN
                     TSAGRKSTQDAYLIHHGALSYNVIYTTTLAGARALCAAARALKEEGWGVCPLQEYYKM
                     "
     gene            732..2159
                     /gene="purF"
                     /locus_tag="PLA58_08390"
     CDS             732..2159
                     /gene="purF"
                     /locus_tag="PLA58_08390"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOR62850.1"
                     /translation="MLPIAEDEQNDPGRPREECGVFAIYGNEDAARVTYFGLFALQHR
                     GQESAGIVASDGCSVSEHKGMGLASGVFNEDILQKLPGSLAIGHVRYSTTGSSTLSNA
                     QPFLVHVGEEYYALGHNGNIVNAQSLRTELEGRGSIFQSTMDSEVIIHLMAPHLQRGL
                     EKALSIALQQVEGAYSLVMLTRNKIIAARDPRGFRPLALGKLNGGWVVASETCAFDLV
                     GAEYIRDVEPGEILMIDDAGCRSIKPFPVMKRCHCIFELIYFARPDSQIFGRNVYLCR
                     KQLGHELAREYRPEVDLVMPFPDSGTYAAIGYAEKSGIPFEMGMIRNHYVGRTFIQPT
                     QPMRDFGVRVKLNPVRPLLAGRKVMIVEDSVIRGTTSRNRVKNLREIGVKELHMVISC
                     PPTLFPCAYGIDFSSKGELIAAKKETEQAIAEFISLDSLHYLTVEGMVRATGLPKENF
                     CLACYTGDYPIAPPEKIDKFCMELK"
     gene            2234..2806
                     /locus_tag="PLA58_08395"
     CDS             2234..2806
                     /locus_tag="PLA58_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOR62851.1"
                     /translation="METFLSIAVGIGLAAACGFRVFVPLLILNLASLAGALTLPSGFA
                     WMGSPAATVAFATATAAEIIGYYVPWFDHALDTIATPAAIAAGTLTTAAFLTDVSPFL
                     KWTTALIAGGGTAGLIQASTVVLRAKSSLATGGAANPLFATMEIAGALVTGILAVLLP
                     VVCLVLVLIFCLWAVRKVIRFFSEKNTGSP"
     gene            complement(2866..3231)
                     /locus_tag="PLA58_08400"
     CDS             complement(2866..3231)
                     /locus_tag="PLA58_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR62852.1"
                     /translation="MTFAGFDFSQGDRTSYDRFTDAFPLDPASPQQAFLAFDGGKPVA
                     ASLLFLAGDAAGLYFITTLGDYRRRGIGREMVLATLHYARAAGARFASLQSSPDGLSV
                     YEKAGFKECCRVDIYGRKA"
     assembly_gap    3552..3651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3652..3998)
                     /locus_tag="PLA58_08405"
     CDS             complement(<3652..3998)
                     /locus_tag="PLA58_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62853.1"
                     /translation="MDETGPYRLVTPMSLVRIIEDTFFSVCRYWGGLQNAFDCFGPVR
                     AMQTGIGSADLNMVWSEGPLTREDAPWVRQVETYYDQANLPYWWWIFPRSKTAETIRL
                     LKDTGHVYLLSMP"
     gene            complement(3968..4609)
                     /gene="pdxT"
                     /locus_tag="PLA58_08410"
     CDS             complement(3968..4609)
                     /gene="pdxT"
                     /locus_tag="PLA58_08410"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOR62854.1"
                     /translation="MGVLDLQGGVHEHLEHLERLGLPGRRVKQAVDFENLAGLILPGG
                     ESTCLARLLSVFGLADVIRRHVGRGMKIWGTCAGAILLATEVSGEKPCLGLIEMKIVR
                     NGFGSQLDSFHSEALIEAVSPEPVPLTFIRAPKILAVGKDVRVLLRMDDYIAAAENDR
                     VLVTVFHPELTPSLALHRYFALKCGLTVAAETSAPAGSGWENESWMKLARIAS"
     gene            complement(4655..5536)
                     /gene="pdxS"
                     /locus_tag="PLA58_08415"
     CDS             complement(4655..5536)
                     /gene="pdxS"
                     /locus_tag="PLA58_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOR62855.1"
                     /translation="MDAKRYELNVQLAQMLKGGVIMDVTNVEQAKIAEEAGAVAVMAL
                     ERVPADIRKDGGVARMSDPAMIAEIKKAVTIPVMAKARIGHFVEAQLLEALRIDYIDE
                     SEVLTPADEECHIDKTKFSIPFVCGARNLGEACRRIAEGAAMIRTKGEAGTGNVVEAV
                     RHMRTMNREIRQLAQMPVEEVTGFAKTNGIPYELALKVRTLGRLPVVNFAAGGIATPA
                     DAALMMQLGCDGVFVGSGIFKSADPARRARAIVKATAYFNDPEKVLEASMDLGQAMPG
                     LEIGQIPPEQILAKRGW"
     gene            complement(5657..6436)
                     /gene="hisF"
                     /locus_tag="PLA58_08420"
     CDS             complement(5657..6436)
                     /gene="hisF"
                     /locus_tag="PLA58_08420"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOR62856.1"
                     /translation="MISKRIIPCLDVRDGKLTKGIKFKGNIDIGDPVEAAREYYEQGA
                     DEIVFYDITASSDKRDIMIDVVRRVAETIFIPFSVGGGIRNLDDMRRVIGAGAEKVSV
                     NSAAVMEPAIISQGARAFGSQCVVLGMDVKKTAVSNQTPSGYEIVIEGGRTFTGIDAL
                     WWAGEAQRLGAGEICLNSIDADGVQNGYELDLTALISENVRIPVIASGGAGKPAHLAD
                     VLTKGRADAALIASMVHYRTYTIRQIKQYLSDRNIATRMFW"
     gene            complement(6433..7059)
                     /gene="hisH"
                     /locus_tag="PLA58_08425"
     CDS             complement(6433..7059)
                     /gene="hisH"
                     /locus_tag="PLA58_08425"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOR62857.1"
                     /translation="MIAIIEYNAGNITSVARALHAIGQEFIITNDTKMLDAASCVIFP
                     GVGAAGAAMDYLKEKKLDVWLKNCFRSGKPILGICLGTQIVLDTSEENDTTCIGLVGG
                     TTRRFPENLAADGQALKIPHMGWNSVTFRRPHPVFAGVPAQAEFYFVHSFYPSPADPS
                     AVLGTTEYGMTFCSVLAVNNLVAVQFHPEKSGRPGLQILKNFCAWEGK"
     gene            complement(7173..7547)
                     /locus_tag="PLA58_08430"
     CDS             complement(7173..7547)
                     /locus_tag="PLA58_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOR62858.1"
                     /translation="MNVVDSSAWLAYFADEPNAKHFMTPLSHPATLIVPAITIYEVFK
                     VILRESSENEALQAVVAMQKGKVIDLNTSLALAAAKLSLEHQLPMADSIILATAQEFK
                     ATIWTQDVDFKGIGKVKYFPKQ"
     gene            complement(7544..7750)
                     /locus_tag="PLA58_08435"
     CDS             complement(7544..7750)
                     /locus_tag="PLA58_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOR62859.1"
                     /translation="MQSVTVSPKYQVVIPKNVRESLKLQPGQKMQIVEYAGRIELIPE
                     RDIKELRGFLKGINTEFKREEDRL"
     gene            complement(7981..8454)
                     /locus_tag="PLA58_08440"
     CDS             complement(7981..8454)
                     /locus_tag="PLA58_08440"
                     /inference="COORDINATES: protein motif:HMM:NF040101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6429 family protein"
                     /protein_id="HOR62860.1"
                     /translation="MNYDEDKIDEYTLALLYLVTHDRQEGFGARAWKGFDWDTMNRLY
                     EKGYISNPIGKAKSIAMTEEGFLKSEELFERHFLKKIQPIPFPKMTPPAKKRWDEIPE
                     QMRKKILENVWCSKCLTMVKLQLREGRMSGRSLVLKGVCNTCGGEAARVIEPVEE"
     gene            complement(8564..>8837)
                     /locus_tag="PLA58_08445"
     CDS             complement(8564..>8837)
                     /locus_tag="PLA58_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62861.1"
                     /translation="YYGKLNHCRFPDNNVEHFFHLPIDSQIRDHLVTKCSFTDPTPLP
                     WSRWARSHYVSFQAQVRKRISSEYKPLEIDYILWNTQGASLGGVMR"
BASE COUNT         1895 a         2529 c         2321 g         1992 t
ORIGIN      
        1 aggccatcgg cgtgccgctg gcgaagcttg ccacccgggt catgctgggc aaaacattag
       61 gcgagctgtc ttttacagaa accattattc ccaaacacgt ctgtgtgaaa gaagcagtct
      121 ttccgttcaa tcgcttcccc ggcgtggaca tcattctggg gccggagatg aagtccaccg
      181 gcgaggtgat gggcatcgat cattccttcg gtcttgcctt tgccaaagcg cagatggcca
      241 ccggctttaa aatgccgacg gccggcagcg tcttcatcag cgttcacgat tgccacaagg
      301 caggcatcgt ggacgtggcc aggacattcg ccgatctcgg tttcaggatt ctggcaacgg
      361 ccggaacggc cggatacctg aacaggcagg gcatcattgc agccgcggtc aacaaggtca
      421 gtgagggccg tccgcatgtg gtcgatttga tcaagaacgg cgatattcaa atggtgatca
      481 acaccagcgc cggacgcaag tccacgcaag acgcctacct gatccatcac ggcgctttat
      541 cgtacaatgt catttacacc acgacactgg ccggcgcgcg ggctctctgc gcagcggcca
      601 gggcgctgaa agaggagggc tggggggtat gccccctgca ggagtattat aaaatgtagg
      661 gaacggtttt aaaccgttcc ctacgctggc cccctgcaca taccgggtga ggggaaaatt
      721 ggaaaagtga aatgttaccc attgctgaag acgaacaaaa tgatcccggc cggccccgcg
      781 aggagtgcgg cgttttcgct atttacggaa atgaggatgc cgcccgtgta acgtacttcg
      841 gcctttttgc cctgcagcac cgcggccagg aatcagcggg catcgttgcc tccgacggat
      901 gcagcgtttc cgaacacaag gggatggggc ttgcctccgg cgtcttcaat gaagatattc
      961 tgcaaaaact ccccggctcg ctggccatcg gccacgtgcg ctactccaca accggttcct
     1021 ccaccctctc caacgcccag ccctttctgg ttcatgtcgg cgaggaatac tatgcgctgg
     1081 gacacaacgg caatatcgtg aacgcccagt ccctgcggac cgaactggaa ggccggggat
     1141 ccattttcca gtccacgatg gacagcgagg tcattattca tctgatggcc ccgcatctgc
     1201 aaagaggtct ggaaaaggcg ctttccatcg cgcttcagca ggttgaaggc gcctattccc
     1261 tggtaatgct gacccgaaac aaaatcattg cagcccgcga tccgcgcggg tttcgccccc
     1321 tggcactggg aaaactgaac ggcggctggg tggtcgcctc ggaaacctgc gccttcgatc
     1381 tggtgggcgc cgaatacatc cgcgacgtcg aacccgggga aattctcatg attgacgacg
     1441 ccggctgccg gagcatcaaa ccttttcccg tcatgaagcg ctgccactgc atcttcgagt
     1501 tgatctactt tgcccgcccc gacagccaga tcttcggccg gaacgtgtat ctgtgccgca
     1561 agcaactcgg ccatgaactg gccagagaat accggccgga ggttgacctg gtgatgcctt
     1621 ttccggattc cggaacctat gccgcgatcg gctatgcgga aaaaagcggt attccctttg
     1681 aaatgggcat gatccgcaac cactacgtgg gccgcacgtt cattcagccg acgcagccca
     1741 tgcgtgactt cggtgttcgc gtcaaactca atccggtcag gccgctcctg gccggccgga
     1801 aagtcatgat cgtcgaggat tccgttatcc gcggcaccac cagccgcaat cgcgtgaaga
     1861 acctgcggga aatcggcgtg aaggaactgc acatggtcat cagttgtccg ccgacgcttt
     1921 tcccctgcgc ctacgggatt gacttttcct ccaagggcga actcatcgcc gccaaaaagg
     1981 aaaccgagca ggcgattgcc gaattcatca gcctggattc gctgcactat ctgacggtgg
     2041 aaggtatggt ccgggccacc ggcctgccca aagagaattt ctgtctggcc tgctacaccg
     2101 gcgactatcc catcgcgccg ccggaaaaaa tcgacaagtt ctgtatggaa ttgaagtgat
     2161 tttatttcag ccatgccgag gacactccga attcggcttc ctttttttct ccacggagtg
     2221 taaaaaccgg agcatggaaa cttttctgtc catcgcggta ggaatcggcc tggccgcggc
     2281 ctgcggcttc cgggttttcg ttcctctgct tatcctcaac ctggcgtcgc tcgccggagc
     2341 gttgaccctg ccttccggct ttgcctggat gggcagtcct gccgcgaccg tcgcctttgc
     2401 cacggcaacc gcagccgaaa tcatcggcta ttatgttccc tggttcgatc atgcgctgga
     2461 cacgattgca acgcccgcgg ccattgcggc gggaaccctg accacggccg cattcctgac
     2521 ggatgtttcc ccgtttctca aatggacgac cgctctgatc gcgggcggcg gcacggcggg
     2581 cctgatccag gcatcgacgg ttgttctgcg cgcaaaatct tccctggcca ccggcggggc
     2641 cgccaatccc ctgttcgcca cgatggaaat agcgggcgcg ttggtcacgg gaatcctggc
     2701 cgtcctgctg ccggtggttt gtcttgttct cgttttgatt ttctgtctct gggccgtaag
     2761 aaaagtgatc cggttttttt cggaaaaaaa cacgggaagc ccctgagtca aaattttgtc
     2821 cgaacgccgc gggaggcagt gccagaaaaa aaacgctttt gccccttacg ccttcctacc
     2881 gtaaatgtcc acgcggcagc attctttaaa tcccgctttt tcatagacac tcaggccgtc
     2941 cggcgacgat tgaagcgagg caaagcgggc gcccgccgcg cgggcgtaat gaagcgtcgc
     3001 cagcaccatt tcccggccga tgccccgacg gcgatagtcg cccagtgtcg tgatgaagta
     3061 gaggccggcg gcatcgcccg ccagaaaaag cagggaggcg gcgaccggct ttccgccgtc
     3121 aaacgccaga aaagcctgct ggggagaggc gggatcaaga gggaaggcgt cggtgaagcg
     3181 atcataggaa gttctgtcgc cttgcgaaaa atcaaacccg gcaaaggtca cttctttcca
     3241 cggcgacaga tcttgctgat ttccgacccg gcggatgatg accgcaggag atctttgagt
     3301 aactccttga ttcgtcatac cgtcgaaggc cggtatccag gaaccccttg aaaaaactgg
     3361 attcccgcac tcgtgccctc gcgccacctt caggtggttg gggtattcgc gggaatgacg
     3421 attgttgaat ttgtgtggtt gcgcaaagtt ctccgcaggc gcgatggcgg acaagtcgtc
     3481 gagatcggcc agcagacacg gcatactcag cagataaacg tggcctgtat ctttaagcag
     3541 ccggattgtt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacggcata
     3661 ctcagcagat aaacgtggcc tgtatcttta agcagccgga ttgtttccgc ggttttagag
     3721 cggggaaata tccaccacca gtagggcagg ttcgcctgat catagtatgt ttcaacctgc
     3781 cggacccagg gcgcgtcttc ccgcgtcagg ggcccttcac tccagaccat gttcagatcg
     3841 gccgaaccga tccccgtctg catcgcccgg accggaccaa aacagtcaaa agcgttttga
     3901 aggccgcccc agtagcggca gacggaaaaa aacgtatctt caatgatgcg gacaagagac
     3961 atgggggtta cgaggcgata cgggccagtt tcatccaact ttcgttttcc cagcctgaac
     4021 cggcgggggc agacgtctcc gctgcgacgg tcaagccgca tttcagggca aaataccggt
     4081 gcagcgccag gcttggggtc aattccggat gaaagacagt gaccagaacg cggtcatttt
     4141 ccgccgctgc gatataatcg tccatgcgga gcaaaaccct gacgtctttt ccgacggcaa
     4201 gtattttagg cgcgcggata aaggtgaggg gaaccggttc gggtgagacg gcttcgatga
     4261 gcgcttcgct gtgaaagctg tcgagctggc tgccgaagcc gttgcgcacg attttcatct
     4321 cgatcaggcc caggcacggt ttttcaccgg acacctccgt cgccagcaga atggcgcctg
     4381 cgcaggtgcc ccagattttc atgccccggc caacgtgacg ccggatgacg tccgcaaggc
     4441 cgaaaacgct cagcagcctg gccaggcacg tgctctctcc gccgggaaga atcaggcccg
     4501 ccagattttc aaaatccacg gcctgcttga ccctccggcc gggaagccca agccgctcca
     4561 ggtgctccag gtgttcgtga acaccgccct ggagatcgag aacgcccatg atttccggtt
     4621 gaggtctgat caaatgcacc gacataaaat tttgttacca gccccgcttg gccagaatct
     4681 gctcgggcgg aatctggccg atttccaggc ccggcatggc ctgacccaga tccatcgacg
     4741 cttccaggac tttttccgga tcgttgaaat aggcggtcgc cttgacgatg gcgcgcgccc
     4801 ggcgggccgg atcggcggat ttgaaaatgc cggagccgac aaaaaccccg tcgcagccca
     4861 gttgcatcat cagcgccgca tcggcgggcg ttgcgatgcc gcccgcggca aagttgacca
     4921 ccggcaggcg ccccagcgtt ctgactttca gggcaagctc gtagggaatg ccgttggttt
     4981 tggcaaatcc ggtcacttcc tcaaccggca tttgcgccag ctgacggatc tcgcggttca
     5041 tggttctcat gtggcggacg gcttccacga cattgcccgt gcccgcttca cccttggtgc
     5101 ggatcatggc cgcgccttcg gcgatgcggc ggcaggcttc acccaggttg cgggcgccgc
     5161 agacaaaggg aatcgagaat ttcgttttgt cgatgtggca ttcttcatcc gccggtgtca
     5221 ggacttcgct ttcatcaatg tagtcgatgc gcagagcttc cagaagctgc gcctccacga
     5281 agtggccgat tcttgccttg gccatgaccg gaatggtaac ggcctttttg atttcggcaa
     5341 tcatggccgg atcggacatg cgggccaccc cgccgtcctt gcgaatgtcc gccggcacgc
     5401 gctccagcgc catcacggca accgcacccg cctcttccgc gattttggcc tgctccacat
     5461 tggtgacgtc catgatgacg ccgcccttga gcatttgcgc cagctgaaca tttaattcat
     5521 acctttttgc atccactttt tatacgcctc ctgactttgt aaaatcgtct cttcgtgatt
     5581 tcacgaaaat atgatgtaat ataataacgg aatattaaga atacaaacgg attttcgtca
     5641 agcagaatca ttggcgctac cagaacatcc gcgtcgcaat gttccggtcg ctcagatatt
     5701 gcttgatctg ccggatcgta tacgtccggt aatgaaccat cgaggcaatg agcgccgcgt
     5761 cggcccggcc cttcgtcagg acatccgcca gatgcgcagg cttgcccgcg ccgcccgaag
     5821 caatgaccgg aatccgcaca ttctccgaaa taagcgccgt caaatcgagc tcgtagccgt
     5881 tctgaacgcc gtcggcgtca atggaattga ggcagatttc ccccgcgcca agacgctggg
     5941 cctctccggc ccaccaaagc gcgtcgattc ccgtgaaggt tctgccgcct tcaatgacga
     6001 tttcatagcc ggagggggtc tggtttgaga cggccgtttt tttgacgtcc atccccagca
     6061 ccacgcactg actgccgaac gcccgggcgc cctgcgatat gatggcgggc tccatcaccg
     6121 ccgcagagtt cacgctgact ttttccgccc cggcgccgat gacgcggcgc atgtcgtcaa
     6181 gattccggat gccgccgccg accgaaaagg gaatgaaaat cgtttccgcc acgcgtcgca
     6241 cgacgtcgat catgatgtcg cgcttgtccg aggacgccgt gatgtcgtag aaaacgattt
     6301 cgtcggcccc ctgctcgtag tattcgcgag ccgcttccac cgggtcgccg atgtcgatat
     6361 tgcctttaaa tttaatgcct ttggtaagtt tcccgtcgcg gacatccagg cagggaatga
     6421 ttcttttgga gatcattttc cctcccatgc gcagaaattc ttcagtatct gcaggcccgg
     6481 ccggccgctt ttttccgggt gaaactgcac ggcaaccaga ttgttcaccg ccagcaccga
     6541 acaaaacgtc atgccgtatt cggtcgtacc cagcaccgca gacggatcgg caggcgacgg
     6601 gtagaaggaa tgaacgaagt aaaattccgc ttgggccgga acaccggcaa acaccggatg
     6661 cggacgccga aaggttacgc tgttccagcc catgtggggg attttcaggg cctggccgtc
     6721 cgcggccagg ttttccggaa aacgccgggt ggtcccgccg accaggccga tgcacgtcgt
     6781 gtcattttct tcggaggtat ccagaacaat ctgcgtgccc aggcagatgc ccaggatcgg
     6841 ctttccggat cgaaaacaat ttttaagcca cacatccagc tttttttcct tgaggtaatc
     6901 catcgccgcg cccgctgctc cgacccccgg gaaaataaca caggaagcgg catcgagcat
     6961 ttttgtatca tttgtaatga taaactcctg gccgatggca tgcagcgccc tcgccacgct
     7021 ggtaatgttt cccgcattat attcgataat tgcgatcatt ttgctcaaat tccctgaaaa
     7081 ataatcttac gctctccaaa tcctatcagc aaattcaata cccaaaaatc acccggaaca
     7141 cggcaatcgg gtggattgac ttggttgtgc tcttattgct ttggaaaata tttgaccttc
     7201 ccgatacctt taaagtccac atcctgcgtc catattgtcg ctttgaattc ctgggcagtt
     7261 gcaagaataa tactgtctgc catgggaagc tgatgctcca gactcaactt tgccgctgcc
     7321 agcgccaatg acgtgtttaa atcgataact tttccttttt gcatggccac aacagcctgc
     7381 aatgcctcgt tctcactgga ttcccggaga atgaccttga atacttcgta tatcgttatt
     7441 gccggaacga tgagcgtggc cggatgacta agtggtgtca taaaatgctt tgcatttggc
     7501 tcgtcagcaa aataggcaag ccaggcggaa gaatctacaa cgttcatagc ctgtcttcct
     7561 ctcgcttaaa ttccgtgttg attcctttga gaaatccacg aagctcctta atgtcacgtt
     7621 cgggaatcag ttcaatcctt ccggcatatt ccacaatctg cattttttga cccggctgaa
     7681 gctttaatga ttctctgacg tttttgggta tcaccacctg atatttgggc gatacagtaa
     7741 cagattgcat accaacacct ccatcgatac attgcggtat tacaatatca caatcgatgc
     7801 gattttgtcc attgttccat gcacaacagc gaaatcaccc ggagcacagc gatcgggtga
     7861 attgacttgt tgggcgatca tcttatgcac aaagaggggc ggatgttcac ccgcccctct
     7921 ggcgaaagaa aatcttaccg ggagagggcc gaaggctcgc agtccgatgg agcaattgcc
     7981 tcattcttct acgggttcta tcactctcgc ggcttcacct ccgcacgtgt tacacacacc
     8041 tttaagaacc aaagaccgtc ccgacattcg tccctctctc aattggagtt ttaccatagt
     8101 cagacatttt gaacaccaga cgttttccag aatcttcttt cgcatttgtt ccggtatctc
     8161 atcccagcgt ttctttgcgg ggggtgtcat tttgggaaaa gggatgggtt gtattttctt
     8221 caaaaaatga cgttcaaaga gctcttccga ttttagaaaa ccttcctcgg tcattgcgat
     8281 ggactttgct ttgcctatcg gatttgatat gtaacctttc tcgtaaagcc tgttcatcgt
     8341 gtcccagtcg aaacccttcc atgcccgcgc tccgaatcct tcttgcctat catgggtaac
     8401 gagataaaga agggccagcg tgtattcatc tattttgtct tcatcgtaat tcatttatta
     8461 cccttgcgtt caacacacgg ctcagcggga agagccacac ttggctgcga gccgctgaca
     8521 ggagccgatc aatagacgat ttctttgagc catttgatat cccctacctc ataaccccac
     8581 ctaaagatgc tccctgcgta ttccaaagaa tatagtcaat ttctaaaggt ttgtactcgc
     8641 tactgatacg ctttcgtacc tgagcctgaa agctaacata atgagaacga gcccatcgtg
     8701 accacggcag cggcgtcgga tcggtaaagc tgcatttcgt caccaagtgg tctcgaatct
     8761 ggctgtctat aggaaggtga aaaaagtgct cgacgttatt gtctggaaat cggcaatgat
     8821 tgagtttgcc gtaataa
//
LOCUS       DAOSRM010000089         8782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_7611_length_8782_cov_104.259196, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000089 DAOSRM010000000
VERSION     DAOSRM010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7611_length_8782_cov_104.259196"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLA58_08450"
     CDS             complement(<1..653)
                     /locus_tag="PLA58_08450"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR62862.1"
                     /translation="MIPFRKPLLKTLKAATACFMVLVPSQESVVLEGPGSIRKLPDLM
                     KKQNIRKPLVITDRVLMEKHLPAGLFEAFKAAGMPCAVYDGVQPNPSIKNVEEAYGLY
                     IKNGCDAVVGFGGGSSMDCAKVVAGRAVRPRRTADQLGGYFKIMLPVPKKLPRIVAVP
                     TTAGTGAETTSAAVITDVGRNTKYTVNDFLIHPHVIVLDPELTLGLPPFFTAITAMDA
                     "
     gene            938..1546
                     /locus_tag="PLA58_08455"
     CDS             938..1546
                     /locus_tag="PLA58_08455"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR62863.1"
                     /translation="MKERNRNDLIHLRRVQLTRAAYKVVGEKGYSDFTIKDIAREAGL
                     STGLVHYYFKNKEDLLFKLLKGMNANLKNDLGNALSSLEDPREKLLAFCNEAFDLVHK
                     EKAYFSVLVDFLAQINRNKRLRQAMVKLFQSYRDEIEAILQEGAVKGVFTVNDLRFTS
                     VTIVSLIQGNIFQHLIDQEAFDYSASTEKTKRQIFALVMKEL"
     gene            1777..2055
                     /locus_tag="PLA58_08460"
     CDS             1777..2055
                     /locus_tag="PLA58_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62864.1"
                     /translation="MSREYKSGTYCIEVKCPKHKDLEGLTGDAYFKKKKVHCKTCLAW
                     KFFNWAKDNKWRIVHTWPEVSNKQLAAMIKGIDPVRVEDLTEEEIMCL"
     gene            2046..2891
                     /locus_tag="PLA58_08465"
     CDS             2046..2891
                     /locus_tag="PLA58_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR62865.1"
                     /translation="MSLNLQYGPGRNFVRQAVRLAWPIMQAGKRLSNLPFLKWLIYPF
                     FMRPYNEVSSIPLNIEVQRPENVAMPRRVVKRLLANVDELFIFDECICRRHNKVSSPP
                     QDIGCMALGPAIRRIHPSHGRMAAKEEAFRHVDRAAEAGLIANVAHVWIDPVAFQTRF
                     HDLMFICYCDDTNCLYRTNLKNRGPSLRGACQKLPGLSVRVDGDACLGCGACVEQCFL
                     ADIVLQNGKAVIGDSCAGCGRCVERCPHQALSLEIENEDALYDDLVRWIRGVSNLPIQ
                     CAEKG"
     gene            2896..3285
                     /locus_tag="PLA58_08470"
     CDS             2896..3285
                     /locus_tag="PLA58_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62866.1"
                     /translation="MKKFVFLSGLLNVLLGILFTMPSVIREAGVEPPDHPFWLLFPAV
                     FLFSLGIILMVSSRDLENRSTIIFWDGMSRVAAFAGCGWFGLYAGMGLPLVLAALGDL
                     AIALTYFIGLPRVLDRSFLNILLDRRC"
     gene            3314..4201
                     /locus_tag="PLA58_08475"
     CDS             3314..4201
                     /locus_tag="PLA58_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR62867.1"
                     /translation="MGTRPQWYLTFLAKIWKLAYIKPSVPVFGRFMYKIRVNATTPDQ
                     LNISYLPVNAALSVDSLSLPRVILEGMIRRSEHRIITHRCTCRDAWKCKNYDRNIGCI
                     HIGLPTAEEDAAVAHHASVEEAIAHLHRALAAGLVPFIGHVSADNTIWNVSKDRPFTT
                     VCFCCRCCCTNFDYYRHLPPEAQACIHRLHGVTVGVDRDRCIGCGKCAAVCWAGAAVL
                     GGGKSRIDPDRCKTCGVCAQACPQQAICVSVENVEATVNDLLGRINRDVGGLPVASYR
                     ELKAQGEEAALSNPHKDRG"
     gene            4206..4556
                     /gene="hypA"
                     /locus_tag="PLA58_08480"
     CDS             4206..4556
                     /gene="hypA"
                     /locus_tag="PLA58_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOR62868.1"
                     /translation="MHELPVMKSILDIVLQQARIHNVRSVRAVHLEVGRLSDLEEEWM
                     QRYFDYLSKDTIAQEAKIKIQWNPIVFRCEACVKSFDVERENLRDLVCPFCGRKSLLL
                     VSGREYRIKNLEVV"
     gene            complement(4580..5293)
                     /locus_tag="PLA58_08485"
     CDS             complement(4580..5293)
                     /locus_tag="PLA58_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOR62869.1"
                     /translation="MPQEMILLLGTAAFLGFFHTLLGPDHYLPFIVMGKARKWSMLKT
                     SWITLLCGVGHVASSVGLGLIGIALGITVIRLAAIESFRGNLAAWALIAFGLVYFVWG
                     MRRAWKFRPHEHIHVHADGRAHIHPHVHTEEHAHLHEKSGAGHLTPWVLFTIFVLGPC
                     EPLIPLLMYPAAKENLWGLVLVTTVFAASTLLTMLAIVLASSWGLNRLPLGRMERFTH
                     ALAGGSICLCGAAIQFLGL"
     gene            complement(5278..5982)
                     /gene="hypB"
                     /locus_tag="PLA58_08490"
     CDS             complement(5278..5982)
                     /gene="hypB"
                     /locus_tag="PLA58_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOR62870.1"
                     /translation="MNDVRMIEVKEDILADNTRIAEGVRLRLRDTKTLLLNLMSSPGS
                     GKTSLILRTIEGLQGAVKLGVIEADIDSKVDAEKVAAAGIPAVQLRTGGFCHLDATMV
                     TQGIDALPAQRLDLIIIENVGNLVCPAEFDTGAHKNVMILSVPEGDDKPLKYPLMFTV
                     CDVLLINKIDYMAQSDFDVAAARERALALKPGIKIFEVSCKTGAGLSEWINWLKKETE
                     DFLAGFMEKNPPCLRK"
     gene            complement(6010..7128)
                     /locus_tag="PLA58_08495"
     CDS             complement(6010..7128)
                     /locus_tag="PLA58_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR62871.1"
                     /translation="MPVNKETLQRPQMDIYEQLREILDAHPSTAPKGEAIHEILRILF
                     TPEEAAIAVSMSFKPKSPAGLGRSAGVDENKAALLLESMADKGVIFSKNKDGKKFYGL
                     VPLIPGVFEFPFMKGGGSPMHERLARLWEAYHTESLGASFAGNPTPMMRVVAVEKSVT
                     PEHQVRPYDEVRHLIENAGYIALTKCACRVSVAKCDKPKEVCLIFDGVGEFLVERGFA
                     RRISKEEGIRVLDAAEEAGLVHTSNNSADRASVICNCCRCCCTILRARTQLNHPHAFE
                     PSRFAAVVKTEDCIGCGLCAEERCPMQAVEILGDVSFVHEEKCIGCGLCVSTCPSGAM
                     SLMERKQVPAVPATTQDMAIQVLQEKGRLEAFMKVMQS"
     gene            complement(7119..7397)
                     /locus_tag="PLA58_08500"
     CDS             complement(7119..7397)
                     /locus_tag="PLA58_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62872.1"
                     /translation="MEKEYQIGTFCKDIKCRKHKALEGLSGNAYLRKKNVHCADCEAW
                     KFFNWSKEKKFRIVHSLPQMSSKELAARLRGLDIVRVEDLTEDEIMCL"
     gene            7757..8662
                     /locus_tag="PLA58_08505"
     CDS             7757..8662
                     /locus_tag="PLA58_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR62873.1"
                     /translation="MKKDHNPTGKRGRPRPESNTSLRPDSARGTAAIPKDVDPRFCGL
                     RDAVKRGWYRLEAGELFAGFQVSRKDVVLDVGCGEGNAALFCARQGAHVVCSDADAGK
                     IKALAKKMKEAGARKFEAFISDSLPLPLPDAYATKILCTEMLEHTPDPGKIMAELVRV
                     GKPNARYLITVPDARSERLQMHVADPLYFSAPNHIQIFDRERFLRLVEDAGLAVLKYD
                     TWGFYWTVFMSLFWIAKRQEHLQGAILDSIKPPYPPVLQSWANTWAGLMELNGAEKLM
                     ESFDQYLPKTQVIIAEKRKFIGSVI"
BASE COUNT         1893 a         2380 c         2518 g         1991 t
ORIGIN      
        1 gcgtccatgg cggtgatggc cgtgaagaac ggcggcaggc caagcgtaag ctccggatcg
       61 agcacgatga cgtgaggatg gatcagaaag tcgttcaccg tgtacttggt gtttctcccg
      121 acgtcggtaa tgaccgcggc gctggtggtt tccgcgcctg ttccggcggt ggtcggcacg
      181 gccactatgc gggggagctt cttcgggacc ggcagcatga tcttgaaata gcctcccagt
      241 tgatcggcgg tccgtcgggg ccggaccgct cttccggcaa cgaccttcgc acagtccatg
      301 gacgagccgc cgccgaaacc gactacggcg tcgcacccgt tttttatgta cagcccgtag
      361 gcttcctcaa cattttttat ggaagggttc ggctgcacac cgtcatagac cgcgcagggc
      421 atgccggcgg ctttgaatgc ctcaaacagg ccggcgggca gatgtttctc catcagcaca
      481 cgatctgtaa tcacaagcgg tttccggatg ttctgttttt tcatcagatc ggggagcttc
      541 cggatgcttc ccggtccctc aagcaccaca ctttcctgtg aggggacaag aaccatgaag
      601 catgccgtgg cggctttcag ggttttgagc agcggttttc gaaaaggaat catttccgaa
      661 tttctccttg tgcgaaaggc atcgtgcctt tgaggtgacg tcactataag aggaatgaac
      721 aggcctgtca acgaaaagac gcgatccgtc ttcatgttgc agtaatattt cattgacatc
      781 aaagtgaagt tttcctatgc tccctttccc gtaaagacgg acgcctcttc aggaggcgct
      841 gccccacgat gcgccggaaa attccggtcc ttcgcaaggc ggatcaaaaa tccgggcgtg
      901 atgaaaaaaa agatttgttg ctccctgcag gaatgtgatg aaagaacgaa acagaaatga
      961 tttaatccat ctgcgacggg tccagttgac ccgggcggca tacaaagtgg tcggtgaaaa
     1021 gggctattcg gatttcacga tcaaggacat cgcgagggaa gccggcctct ccacgggtct
     1081 ggtccactat tacttcaaga acaaagagga tctcctgttt aaactgttga aggggatgaa
     1141 cgccaatctc aaaaatgatc tgggcaacgc gctgtcatca ctggaagatc cgcgggagaa
     1201 gcttttggct ttctgcaacg aggcgttcga tctggtgcat aaggaaaaag catatttcag
     1261 cgtgctggtc gattttctgg cgcagattaa ccgcaacaaa cggctgcgtc aggcgatggt
     1321 gaagcttttt cagagctacc gcgatgagat tgaggccatt cttcaggaag gcgcggtcaa
     1381 gggcgtgttt acggtcaacg atttgcgctt tacatcggtc accatcgtgt ccctgatcca
     1441 ggggaatatt ttccagcacc ttattgatca ggaggctttc gattattccg cctctaccga
     1501 gaaaacgaaa agacagattt ttgccctggt catgaaggaa ttataacgcg ttcaatgaag
     1561 gccttccggg acggggcggc aaagaatgca ggggatcgat gccggacctg ccggccgctt
     1621 ccgcagacgt caaattcctt gccaaactat cgcttgacaa cccaaaacgg acaccctata
     1681 gttttacgcc aaaatagggc attcgttgaa ttcgacaggc ctgttaaccg tttcgtctcc
     1741 ggcgtcggcc ggcagcggag aaaagaggga gtatccatga gcagagaata caaatcgggt
     1801 acatactgca ttgaggtaaa atgccccaag cacaaggatc ttgagggcct taccggggac
     1861 gcctacttca aaaaaaagaa ggtgcactgc aagacgtgtc tggcctggaa atttttcaac
     1921 tgggcaaaag acaacaaatg gcgcattgtc catacctggc ccgaagtttc caacaaacag
     1981 cttgccgcca tgatcaaggg gattgatccg gttcgggttg aggatttgac cgaagaagag
     2041 atcatgtgtc tttaaacttg caatacggcc ccggcaggaa tttcgtcagg caggccgtcc
     2101 ggcttgcgtg gcctatcatg caggcgggca aaaggctcag caatctcccc ttcctgaaat
     2161 ggttgattta tccttttttc atgcggccat ataatgaagt gagctccatc cccctcaata
     2221 ttgaggtcca gcgccccgaa aacgtcgcga tgccccgccg cgtcgtcaaa agacttctgg
     2281 cgaatgtgga cgagctgttc atcttcgacg aatgcatctg ccgaaggcac aacaaagttt
     2341 cgtctccgcc gcaggatatc gggtgcatgg cgctgggtcc ggccattcgc cggattcatc
     2401 ccagccacgg aagaatggcc gccaaagagg aagccttccg ccatgtggat cgcgccgccg
     2461 aagcgggcct gatcgccaac gtcgcccatg tgtggattga tccggtcgct tttcagacga
     2521 gattccatga tctcatgttc atctgttact gcgacgacac caactgcctg tatcgcacga
     2581 atctgaagaa tcggggcccg tccctgcggg gcgcctgcca gaagcttccc ggcctgtcgg
     2641 tccgggtgga cggcgacgcc tgcctgggct gcggcgcctg tgtggagcag tgtttccttg
     2701 cggatattgt tttgcaaaat ggaaaggcgg tgatcggaga ttcctgcgcc ggctgcggcc
     2761 gctgcgtgga aagatgcccg catcaggccc tctcgctcga gatcgaaaac gaagacgccc
     2821 tgtatgacga cctggttcgc tggattcgag gggtttccaa cctgccgatc caatgcgcgg
     2881 agaaaggatg atgacgtgaa aaaatttgta ttcctgagcg gccttttaaa tgtcctgctg
     2941 ggcatccttt tcacgatgcc ctctgtgatt cgcgaggcgg gcgtggagcc gcccgaccat
     3001 cccttctggc tcctgtttcc cgccgttttc cttttttccc tcggcatcat tctgatggtt
     3061 tcctcgagag atctggaaaa tcgctccacc attatttttt gggacggcat gtcgcgcgtc
     3121 gcggcctttg ccggctgtgg ttggttcggg ctgtatgccg ggatggggct tcctctcgtg
     3181 cttgccgcgc tgggcgatct ggccatcgcg ctcacgtatt ttatcggact gccccgcgtt
     3241 ctcgatcgaa gcttcctgaa cattcttctg gaccggcgct gttgaggatg ccggataagg
     3301 aaagggattt tccgtgggaa ctagacccca gtggtacctt acctttctgg cgaaaatctg
     3361 gaagctcgcc tacatcaaac cgtccgtgcc ggttttcggc cggttcatgt acaaaatccg
     3421 ggtgaacgcg acaacgccgg atcagttgaa tatttcctat ctgcccgtca acgcggcgct
     3481 cagcgtggac agcctttccc tgcccagggt cattctggaa ggcatgatcc gccgctcgga
     3541 gcaccgtatt attacgcatc ggtgcacgtg ccgggacgcc tggaaatgca aaaactacga
     3601 ccggaacatc ggctgcatcc acattggact ccccacggcc gaagaagacg ccgccgtggc
     3661 gcaccatgcg tcggtggaag aggcgattgc gcatctgcac agggcccttg ctgcggggct
     3721 ggtccctttc atcggacatg tgtcggcgga caatacgatc tggaatgttt ccaaagaccg
     3781 gccgttcacc acggtttgtt tctgctgccg gtgctgctgc acgaatttcg actattaccg
     3841 ccatcttccg cccgaggctc aagcctgcat tcatcgcctt cacggggtga cggtcggcgt
     3901 ggatcgggac cgatgcatcg gctgcggcaa atgcgccgcg gtatgctggg ccggcgccgc
     3961 cgttctcggc ggcggaaaat cacgaatcga ccccgatcgg tgcaagacgt gcggcgtctg
     4021 tgcgcaggcg tgcccgcagc aggcgatttg cgtttccgtg gagaatgtcg aggccaccgt
     4081 caacgacctt ctggggcgga tcaaccgcga tgttgggggg ctgccggtcg catcctatcg
     4141 ggagctgaag gcgcagggag aagaggccgc tttgtctaat ccgcacaaag atcggggatg
     4201 aacgaatgca cgaattgccg gtgatgaaga gtattctgga tattgtgctg cagcaggcca
     4261 ggattcacaa cgtccggagc gtccgcgccg tccacctgga ggtcggccgg ttgagcgacc
     4321 tggaagaaga gtggatgcag cgctattttg attatctcag caaagacacg attgcccaag
     4381 aggcgaaaat caaaatccag tggaatccca ttgtgttccg gtgcgaagcc tgcgtcaaat
     4441 cctttgacgt tgaacgggaa aatctgcggg atttggtctg tccattctgc ggccggaaga
     4501 gtctgttgct ggtttccggc agggaatacc gcatcaaaaa tctggaggtg gtgtaaatca
     4561 acccctcaga aagaaatcct tataatccca gaaactgaat tgccgcgccg caaagacaga
     4621 tcgatccgcc ggccaacgcg tgggtgaatc gctccattct tccgagcgga aggcggttga
     4681 gcccccagga agaagcaagg acaatggcga gcatggtcaa aagggttgag gcggcgaata
     4741 cggtcgtgac gagaaccagc ccccacagat tttcctttgc cgccggatac atgaggagcg
     4801 gaatcagtgg ctcgcagggt ccgagcacga aaatggtaaa aagcacccag ggcgtgagat
     4861 ggcctgcgcc gcttttttca tgaaggtggg cgtgttcttc cgtatgcaca tgcgggtgaa
     4921 tatgcgcgcg gccgtcggcg tggacatgga tatgttcatg aggcctgaac ttccaggctc
     4981 ttcgcatgcc ccagacaaaa tacacgagcc cgaaggcaat caacgcccac gcggccagat
     5041 tgccgcgaaa ggattcgatt gcggccaatc ggattaccgt gatccccagc gcgatgccga
     5101 tcaaccccag tccgacggag ctcgccacat gccctacacc gcacagcaag gtaatccagg
     5161 aggtcttcag catcgaccat ttccgggcct ttcccatgac aataaaaggg agataatggt
     5221 cgggacccag cagggtgtgg aagaagccga gaaacgcggc ggttccgagc agaaggatca
     5281 tttcctgagg catggcgggt ttttctccat gaagccggcc agaaagtctt ccgtctcttt
     5341 tttcagccag ttgatccatt ccgaaaggcc cgcgccggtc ttgcaggaaa cttcaaaaat
     5401 ttttatgccg ggcttcagcg ccagcgccct ttcgcgcgcc gcggctacat caaaatcact
     5461 ctgcgccata taatcgatct tgttgatcag caggacatcg cagaccgtga acatgagcgg
     5521 gtatttgagc ggcttgtcgt cgccttccgg aacgctgagg atcattacgt ttttgtgggc
     5581 gccggtatcg aattcggcgg gacaaacgag gttgccgacg ttttcaatga tgatcagatc
     5641 cagacgctgc gcgggcaggg catcaatgcc ctgcgtcacc atggtcgcgt ccagatggca
     5701 gaatccgcct gttcgcagtt gaacggcggg aattccagcg gccgccactt tttccgcatc
     5761 gactttggaa tcgatgtcgg cttcgatgac gccgagtttc acggcgcctt gcaggccttc
     5821 gatcgtgcgg aggatgaggc tggtcttgcc cgagccgggc gaggacatca ggttcagcag
     5881 cagcgttttc gtgtcgcgga ggcgcagacg aacgccctcg gcaatccggg tattgtcggc
     5941 caggatgtct tccttgacct cgatcattct cacgtcattc acgagcgcaa cctctccgtt
     6001 tttcgtatgt cagctttgca tgactttcat aaaggcttcc agtcttccct tttcctgaag
     6061 aacctgaatc gccatatcct gcgtcgttgc tggaaccgcc ggaacctgtt tcctctccat
     6121 cagactcatg gcgccggagg gacaggtgga aacgcacaga ccgcacccga tgcatttctc
     6181 ttcatgaacg aaggagacat ccccgaggat ctcgacggcc tgcatcggac agcgctcttc
     6241 ggcgcacaga ccacacccga tgcaatcctc tgttttaacg acggctgcaa atcggctggg
     6301 ctcaaaagcg tggggatgat tcaactgcgt tctcgcgcgc aggatcgtgc aacagcaccg
     6361 acaacagttg cagatcacgc tggcccggtc cgcgctgttg ttgctggtgt ggaccaaccc
     6421 ggcttcttcc gccgcatcca gaacccggat gccctcttcc ttgctgatgc ggcgggcgaa
     6481 cccgcgctcc acgagaaatt cacccacgcc gtcaaagatc agacacacct ctttcggttt
     6541 gtcgcatttg gcgacgctta cccggcaggc gcattttgtc agagcaatat agccggcgtt
     6601 ttcgatcagg tgtctgactt cgtcgtaggg gcggacctga tgctccggcg taacggattt
     6661 ttccacggcg accactctca tcatcggtgt cggattgccg gcaaaggaag cgccgaggct
     6721 ttccgtatgg taggcttccc agagccgggc caggcgctcg tgcatcgggg agccgccgcc
     6781 cttcatgaaa ggaaactcga agacgcccgg aatgagaggg accaacccgt agaatttttt
     6841 gccgtccttg ttttttgaaa aaataacgcc tttatcggcc atggattcca gtaaaagggc
     6901 ggccttgttt tcatcaacgc cggcggaccg gccaagaccg gccgggcttt tcggcttgaa
     6961 actcatgctg acggcaatgg ccgcctcctc cggtgtaaag aggatgcgca gaatttcatg
     7021 aatggcctct cccttgggcg cggtcgaggg gtgcgcatcc agaatttccc gaagttgctc
     7081 gtaaatgtcc atctgcggtc tctgaagcgt ttccttattt acaggcacat gatttcgtct
     7141 tccgtcaggt cttcaacccg aacgatgtcc aggcctctga gcctggcggc gagttctttg
     7201 ctcgacattt ggggcaggga atgaacaatg cggaatttct tttccttcga ccagttgaag
     7261 aatttccagg cttcacaatc cgcgcagtgc acgttcttct ttcggagata ggcatttccg
     7321 ctgagtcctt cgagcgcttt gtgttttcga cacttgatgt ctttgcaaaa ggttccgatc
     7381 tggtattctt tctccatgtc caactcctct tttctccgga gccggcggtt gcctgcccgg
     7441 caggttgagg ggccgtgttt ttgccggcgc cctttttgtg ttgaatttta taggcggtat
     7501 gattttgctt gtcaagcgtt agttcaaata cccaaaatcc tgttttgccc tgcggcgttt
     7561 ttgtgctaca cgacacccaa cccgagtctc ccggattcat cacgcggaag cggcggccgg
     7621 caggctttgg atgaggggga ggatatgaca gcccgttgag gcgtcttctt ttcttgaaat
     7681 gtgagtcaac ccgttttcat gacggtgaag gctaatgaaa ttgcgcggag taggtttcgc
     7741 ggggaggatg ccgcctgtga aaaaggacca taacccaacg ggaaaacgag gccggcctcg
     7801 gcctgaatcc aacacaagcc tcaggcccga cagcgcccgc ggaaccgcgg cgattccaaa
     7861 ggacgtggat ccccgcttct gcggcctgcg ggatgccgtg aaacgcggct ggtaccggct
     7921 ggaggccgga gagctgttcg cgggctttca ggtttcccgg aaggatgtgg tgctggacgt
     7981 ggggtgcggg gaagggaacg ccgcgctgtt ttgcgccagg cagggggcgc atgttgtttg
     8041 ctccgatgcg gatgccggaa aaatcaaagc gcttgcgaag aaaatgaagg aagcgggcgc
     8101 gagaaagttt gaggcgttca tcagcgattc gctgccgctt ccgctgccgg acgcatacgc
     8161 gacgaaaatt ctctgcacgg aaatgctgga gcacacgccg gaccccggta aaattatggc
     8221 ggagctggtc cgggtgggca agccgaacgc ccggtatctg atcaccgtgc cggatgcccg
     8281 gtcggaaaga ctgcaaatgc atgttgcgga tccgctctac ttttcagcgc ccaaccatat
     8341 tcagatcttt gatcgggaaa gatttctccg actggtggaa gatgcgggac tggcggttct
     8401 gaagtacgat acctggggat tttactggac ggtcttcatg tctctgtttt ggatcgccaa
     8461 gcggcaggaa cacctgcagg gcgccattct ggacagcatc aagcctccct atcctccggt
     8521 tttgcagagc tgggccaata cctgggcggg attgatggag ttgaacggag ctgaaaaact
     8581 catggaatcc ttcgatcaat atttacccaa aacccaggtc attattgccg aaaaaagaaa
     8641 attcatcgga tccgtcatct aaatccgacg gtgctcttgc cgtaatggtt ccatctttca
     8701 atgacgtaaa aatgtatttt tttgacatct ctgaagtttt tgacgggcag gttgggacac
     8761 agattaagga aggcaagata aa
//
LOCUS       DAOSRM010000090         8661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_7744_length_8661_cov_109.052405, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000090 DAOSRM010000000
VERSION     DAOSRM010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7744_length_8661_cov_109.052405"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1353)
                     /locus_tag="PLA58_08510"
     CDS             complement(103..1353)
                     /locus_tag="PLA58_08510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR62874.1"
                     /translation="MFDYLLSKEQLKIRDEAREFVKSIPRQTILDMDADNIKFPKEFL
                     SEAGKRNLLGCRYPKKWGGREMDWVTTCMLMEEIGTLGYEMACVFGVGAELVCDAIIL
                     HGTDEQKEKYVKPLLRGDLFAAECLTEPRGGSDFFGATTKAEDMGDYFLINGQKRFIV
                     GGEGADFFLLYAKTDFDPKTKPQKSITAFIVDRGPGVETKYLYGLMGCRGGGTARLVF
                     KDAKVPKANVVGKVNGAYDVFNTMMIPERLGTAAMTIGAARPALEVATNYTTRRKAFG
                     QVIAQFEGVSFQVAEAIMLLDAARSMAYVTARAVDGGVNMNRIRRMVSESKKFITESC
                     QKVVHNSMQVCGGIGYTNILPIERIHRDVRLASIWTGTSEVMSMITAHEWYREYLMAK
                     AAGLTRDCELDAAEAEAADEIIYE"
     gene            complement(1383..2891)
                     /locus_tag="PLA58_08515"
     CDS             complement(1383..2891)
                     /locus_tag="PLA58_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR62875.1"
                     /translation="MNLVELLDSTARKNPNRDCIRYMGKGYTYASILNAAEKAAGLFQ
                     QWGLKKDDKVAIMSLNTPSFVIAFYGVLKAGGVVVPVNHKLMSPEVQYILQHSEAKIF
                     LFDGALAAVASKLTGGSVRCLSLDSPAEGFDQFDPLLANAPSFHAVPISENDFAEILY
                     TSGTTGKPKGCLHTHRSVIMSGITGALTMKFDEDDRLLMAMPIWHSSPLNNWFMAAQY
                     VGCTTVMIREYHPLHFLQAIQNEKCTIYFGAPISYVMPVQMIPNFDTFDLSSMRAWIY
                     GGGPIAADTVGMLMSKYKSSLFYQVYGMTESGPTGTTLLPKDQVRKAGSIGHGGLPGC
                     DVMVMKSDTEAAKPGETGEIWLKADSMMKGYFKNPEATAEAFSGPWYRTGDVARLDED
                     GYLFIVDRTKDMIVTGGENVYSKEVEDAIAGHPDVMESAVIAVPHPEWGETVAAIIVP
                     AKGKTLTAESLSAFLADKLAKFKIPRTFRFVESLPHTPSGKIMKYKLREEHQ"
     gene            complement(3156..4298)
                     /locus_tag="PLA58_08520"
     CDS             complement(3156..4298)
                     /locus_tag="PLA58_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR62876.1"
                     /translation="MISLEPSEKQIALQEHFRRLAVKRLRPRSILIDAAKPAGVDPDY
                     RALIAEENLNAFLIPEEYGGKSLDWMSLAILFEELGAGCAGFAAVYAQTFHSIAALLI
                     GGSEEQKKRFFPLLLPPGGHMASFCVTEEKSGSNTSSFSTVFSRKGRSFLLNGAKEPI
                     INAGQSRFYVVWANEEGARNRAGISAFLIPAGTEGLDFGPYHDKPGFRAAPTAAVHFR
                     NVRIPEGNMIGPAGSGYLLLMQILDWGRALGAAIAVGLARTAIEEAVRFAKQRVILKR
                     PIIRNQSIAFMLADLVTELEAARALVWRACRLIDLGKDHTVASSMAKLYAGELAVRAA
                     TEGLLILGQSGFKRPSLMDKLHRDAQAFRIAEGTSHVQKLIISGQL"
     gene            complement(4295..4660)
                     /locus_tag="PLA58_08525"
     CDS             complement(4295..4660)
                     /locus_tag="PLA58_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR62877.1"
                     /translation="MKAYEQIGRRLQQAREEAGMSQEELANVLGYTQSSLSNYELGKR
                     RLYFTDLQRIGQILGKPITYFLDDPNEETVELSDPGTILRGPYLKDILHASRDLKPAQ
                     RKAVLDYILWQKSKMGGDK"
     gene            complement(4819..5619)
                     /locus_tag="PLA58_08530"
     CDS             complement(4819..5619)
                     /locus_tag="PLA58_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOR62878.1"
                     /translation="MTYKHIDLKIENYVATVTMARVEALNALNLEFASEIASVFRELG
                     ANDDVRAIIFCSKARIFCAGLDLKEAAAGASNQAKVVLDVIRKSQPLFDCCNFIEECP
                     KPVIAAVHGKCIGGGLDMISACDIRLATQDAVFCLKEAAIGLVADMGVLQRLPKIVGQ
                     GFAREMAYTAANYTARDVEKKGLLNGIYPDQESLMAAAKKLAAQIAANAPLGIQYTKE
                     VLNFSRYVNVYEGMALAVQKNAILLMSDDLKEAVISFLEKRPADFKGK"
     gene            complement(5704..6849)
                     /locus_tag="PLA58_08535"
     CDS             complement(5704..6849)
                     /locus_tag="PLA58_08535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOR62879.1"
                     /translation="MISKRASGFTPFIVMDIMAKAEEMERKGEHIIHMEVGEPDFPTP
                     KAVIDAAIVALKNNKTRYTHALGLLELRQAICDFYLREYGVSISPDQVLISTGTSPVL
                     LFAMLALLDHGDEVIVSNPRYPCYQNFILAAGGRLREIQTYPEEGFQYRHQDIAKKLS
                     RRTKGIMINSPCNPTGIVMSREQLQDIARFDAPYIISDEIYHGLVYRDRAHSILEFTD
                     RAFVINGFSKLYAMTGWRLGYLIFPKKFSAVMQRIHQNFMISAGGFIQWAGIAALAKT
                     KTAVRTMIRTYDERRRYMLARLTDMGFVIHVEPTGAFYVFADGRRFFTDSYQEAFRIT
                     EKAKVGVTPGIDFGSGGEGFLRFSYATSLENIRKGLDRLERFLKKKK"
     gene            7216..7473
                     /locus_tag="PLA58_08540"
     CDS             7216..7473
                     /locus_tag="PLA58_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62880.1"
                     /translation="MKRLVLLVVAFVFALSMTAVAADKAAPAAAPADKAVAADKATPD
                     ADKKADVKKPAKKAKKAKKAKKAEKKAEEAAPAAPAAPAAK"
     gene            complement(7562..>8661)
                     /locus_tag="PLA58_08545"
     CDS             complement(7562..>8661)
                     /locus_tag="PLA58_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR62881.1"
                     /translation="DTGIGIPADKIHNIFERFTQADSSTTRKYGGTGLGLTISKSIVD
                     LMGGTISVESKEGRGSIFSFTAPFAMQTEMTPAEDACAPVDLTGRRALVIDDNANNRL
                     ILREMLTQWEIVVSSANSGPAGLTALREAQEAGKPYDFVILDYQMPGMDGLMTAVEIR
                     KEPKWSSLAIIMLSSGYPQEDIAAAKKVGIERFLYKPVKRNDMQEAICGELGKKEAAQ
                     PQAETAPAASGAQKSLNILLVEDNEDNRLLISTYLKNTPHRLQMAENGAIAVEAFKSA
                     KYDIVLMDMQMPVMDGYAATREIRKWEKSQGKTAVPIIALTAYALKEDEKKSLDAGCT
                     GHMTKPIKKVKLLEALAEYAAAETGAASKAS"
BASE COUNT         1807 a         2388 c         2385 g         2081 t
ORIGIN      
        1 cgaaattttt gctgctgaag ccgactccca gcctgtgcaa gcgaccctga aggggaatca
       61 gggcgacacg catcgcgttt caccctgatt cccccgttgt cgttattcgt atatgatttc
      121 atccgcggct tcggcttccg cggcatcgag ttcacagtca cgggtcaggc ccgccgcttt
      181 tgccatcaga tattccctgt accattcatg ggcggtgatc atggacatga cctcgctcgt
      241 tccggtccag atggaggcca gccgcacgtc acggtggatg cgttcgatgg gcagaatgtt
      301 cgtgtagccg attccgccgc agacctgcat ggaattgtga acgaccttct ggcaggactc
      361 ggtgatgaat ttcttggatt ccgacaccat gcgccggatg cggttcatgt tgacgccgcc
      421 gtccaccgcg cgcgccgtta cgtaggccat cgaacgggcg gcatccaaaa gcatgatcgc
      481 ctcggccacc tgaaaactga cgccctcgaa ctgggcgatg acctggccga aggcttttct
      541 ccgggtggtg tagtttgtgg ccacttccag ggcggggcgc gccgcgccga tggtcatggc
      601 cgccgtgccg agccgctccg gaatcatcat ggtgttgaag acgtcatagg cgccgttgac
      661 cttgccgacg acattggctt tcggcacttt ggcgtccttg aagaccagac gcgccgttcc
      721 tccgccccgg cagcccatca ggccgtaaag gtatttcgtt tccacgcccg gaccgcggtc
      781 cacgataaac gcggtaatcg atttttgcgg cttggttttg gggtcgaagt ccgtcttggc
      841 gtaaagcaga aagaaatccg ctccctcgcc gccgacgata aagcgcttct gcccgttgat
      901 gaggaagtaa tctcccatgt cttcggcttt ggtggttgct ccgaaaaagt cggagccgcc
      961 ccggggttcc gtcaggcact ccgcggcaaa aagatcgccc ctcaggagcg gtttgacata
     1021 tttctccttc tgttcatccg tgccgtgaag gataatcgca tcgcagacca gctccgcccc
     1081 cacgccgaag acgcaggcca tttcgtagcc gagagttccg atttcctcca tgagcatgca
     1141 ggtggtgacc cagtccatct cccggccgcc ccattttttc ggataacggc atcccagcag
     1201 attccttttt ccggcttcgc tcaagaattc cttgggaaac ttgatgttat ccgcgtccat
     1261 gtcgagaatc gtctgacggg ggatcgattt gacgaattcc ctcgcctcgt cgcggatttt
     1321 gagctgttct ttggacaaaa gataatcaaa cataatcggt ggttcctttc tattcgtgat
     1381 ttttattgat gctcttccct gagcttgtat ttcatgattt tccccgaagg ggtatgcggc
     1441 agcgactcca caaaacggaa cgtacgggga atcttgaatt tggccagttt gtcggccaga
     1501 aacgccgaga gagattcggc cgtaagggtt ttccccttgg ccggaacgat gatggcggca
     1561 acggtctctc cccattccgg atggggcacg gcgatcacgg cggattccat tacgtccgga
     1621 tggccggcga tggcgtcttc cacttctttg gagtaaacgt tttcaccgcc cgtgacgatc
     1681 atgtcttttg tccggtcaac gataaagagg taaccgtcct catccagacg ggccacatcg
     1741 ccggttctgt accaggggcc gctaaacgct tccgccgtgg cctcgggatt tttgaaatac
     1801 cctttcatca tgctgtcggc cttcagccag atttcgccgg tttcaccggg ctttgcagcc
     1861 tccgtgtccg atttcatgac catcacgtcg catccgggaa gtccgccgtg gccgatggag
     1921 ccggctttgc gcacctgatc tttcggcaga agcgtcgtgc cggtcggtcc cgattccgtc
     1981 atgccgtaaa cctggtagaa gagactggac ttgtactttg acatcagcat gccgacggta
     2041 tccgcggcga tggggccgcc gccatagatc caggcccgca tggaggaaag atcaaacgtg
     2101 tcgaaattgg ggatcatctg aacgggcatg acataggaga tcggagcgcc gaaataaatc
     2161 gtgcatttct cgttctgaat ggcctgcaga aaatgaaggg gatgatattc ccggatcatc
     2221 acggttgtgc aacccacata ctgggccgcc atgaaccagt tgttcagcgg cgaggaatgc
     2281 cagatgggca tcgccatcag cagccggtcg tcctcgtcga atttcatcgt cagcgcgccg
     2341 gtgatgccgc tcatgatgac gctgcgatgg gtatggaggc aacccttcgg ttttccggtc
     2401 gttcccgacg tgtacagaat ttccgcaaaa tcattttcgg aaatcgggac ggcatgaaag
     2461 gaaggggcat tggccagaag gggatcgaat tggtcaaatc cttccgcggg actgtccagg
     2521 gaaagacagc gaaccgaccc tcccgtgagt tttgaggcca cggcggccag ggcgccgtcg
     2581 aaaagaaaga ttttcgcctc actgtgctgc agaatgtact gaacttccgg actcatcagc
     2641 ttgtggttga cggggacgac aacgccgccc gccttgagaa cgccatagaa ggcgatgaca
     2701 aaacttggcg tattgagact catgatggcg actttgtcgt cttttttcaa tccccattgc
     2761 tgaaataatc cagccgcctt ctccgccgca ttgagaatgg aagcataggt gtaacccttt
     2821 cccatgtagc ggatacaatc acggttcgga ttttttcttg ccgtactgtc caacaattca
     2881 accagattca taacgatcct cctgaaatgg tttcactggt caaaccctct ttgatcggga
     2941 tcaaaaaggg caggcattta gccttgttcc ggacgaactt catccgcttc cttgccgcca
     3001 tgatgctgtc ataataaatt atgacgtatt gttatgatag agagcaaaaa aagatcgcca
     3061 caggggcgtc atccattttt tcacaacggt ttgcgatccc taaataccac aggatgtcat
     3121 tttatcaagg attatttacg ccactgtctt tgcaactaca actgtccgga aataatgagt
     3181 ttctggacat ggctggttcc ttcggcgatc cggaaggcct gggcgtcccg atgcagtttg
     3241 tccatcaggg agggtcgttt gaagccgctc tgaccgagaa tcaacaggcc ttccgtggcg
     3301 gcgcgcaccg ccagttcgcc ggcatagagt ttcgccatcg aggaagcaac ggtatgatct
     3361 tttcctaaat caatgagccg gcaggcccgc cagacaaggg cgcgtgccgc ctccaattcc
     3421 gtgacgaggt cggccagcat aaaggcgatg ctctgattcc ggatgatagg ccttttcaga
     3481 atgacgcgct gcttggcgaa acgaacggct tcttctatgg ccgtgcgggc aagaccaacg
     3541 gcaatggcgg cgccgagagc gcgtccccaa tccagaatct gcatgagaag aaggtatccg
     3601 cttcctgcgg ggccgatcat gtttccttca ggaattcgaa cgtttctgaa gtggacggcg
     3661 gcggtcggcg ccgcgcggaa accgggtttg tcgtggtagg gtccgaaatc gaggccttcg
     3721 gtcccggcgg ggatcagaaa cgcgctgatt ccggcgcggt ttctcgcgcc ttcttcattg
     3781 gcccacacca cataaaagcg gctctgtcct gcattgatga tgggctcttt agcgccgttc
     3841 agaaggaagg atcttccctt tcggctgaag actgtagaga aggacgaggt gttggagccg
     3901 ctcttttctt ccgtgacgca gaagctggcc atgtggcctc ccggcggcag caggagcggg
     3961 aaaaaacgtt ttttttgttc ttcggaaccg ccgatgagca gggcggcgat gctgtgaaag
     4021 gtctgggcat agacggcggc gaaaccggcg catcccgcgc ccagctcctc aaaaagaatg
     4081 gccagactca tccagtccag agattttccc ccgtattctt ccggaatcag aaaggcattg
     4141 aggttttcct cggcgatcag agccctataa tccggatcaa caccggccgg tttggcggca
     4201 tcgatgagga tggatcgcgg ccgcaggcgt ttgacggcca ggcgtcgaaa atgttcttgc
     4261 agggctatct gcttctctga cggttccagg gaaatcattt gtcgcctccc atcttgcttt
     4321 tttgccaaag gatgtagtcc agaacagctt tgcgctgtgc gggtttcaaa tcacgggatg
     4381 cgtggagaat gtccttcaga taaggtcccc gcaggatcgt tcccggatcg gagagttcca
     4441 cggtttcttc gtttgggtca tccagaaaat acgtaatggg ttttcccagg atctgaccga
     4501 tccgctgaag atccgtgaaa tacaggcgcc ttttgcccag ttcgtaattg gacagcgagg
     4561 attgggtata ccccaggaca ttggccaatt cctcctggct cattcctgct tcctcacggg
     4621 cctgctggag ccggcgtccg atttgttcgt aggctttcat aacggatatg ccttacggaa
     4681 atttttcagt gtgcccggcg gtcgttttgc caaaacgatg gaaccggttc ggcagaatgt
     4741 cttttcaacg ggaaagcgcg cgacctgcag ggccggtcca ttgaaagatc gaccccgcgg
     4801 gaccgaatgg acaagcaatc atttaccctt gaagtcggcc gggcgttttt ccaggaagga
     4861 aatcaccgct tctttcaagt cgtccgacat gagcaggatg gcatttttct ggaccgccag
     4921 cgccatgcct tcgtaaacat tcacataacg gctgaagttc aggacttctt tggtgtattg
     4981 gatgcccagc ggtgcgttgg ctgcaatctg cgcggccagt ttctttgcgg cggccatcag
     5041 gctttcctga tccggataaa tgccgttcaa taaccctttt ttctcgacgt cccgcgccgt
     5101 gtagttggcc gcggtgtaag ccatttcgcg ggcaaagccc tgaccgacga ttttcggcag
     5161 tctctgcagc acgcccatgt ccgcgaccag gccgatcgcg gcttctttga ggcagaagac
     5221 cgcgtcttgt gtagccaggc ggatgtcgca ggcggaaatc atatccagtc cgcccccgat
     5281 gcattttccg tgaacggcgg caatgaccgg tttgggacat tcttcaataa aattacagca
     5341 gtcaaacaaa ggctgagatt ttcgaattac gtccaggaca accttggcct ggttgcttgc
     5401 gccggcggcc gcttccttta aatccagccc ggcgcagaaa atgcgcgcct tgctgcagaa
     5461 gatgatcgcc cgaacgtcgt cattggcgcc cagttcacgg aagaccgagg cgatttccga
     5521 ggcaaattcc agattcagag cattgagcgc ttcgacacgc gccatggtaa ccgtggccac
     5581 ataattttcg attttcaggt caatgtgctt gtaggtcata tctttttcta tcctccttgg
     5641 ggatgatggt tgcattcgtt gcgatttcat acgctaaaag aacggatttg tctatccgca
     5701 gttctacttt ttctttttga gaaagcgctc cagacggtcc agtcctttcc tgatattttc
     5761 cagagacgtg gcgtaggaga agcgcagaaa accctcgccc ccgctgccga aatcgatgcc
     5821 gggcgtcacg ccgacttttg ccttttccgt gatccggaaa gcctcctgat aagagtcggt
     5881 aaaaaatctg cgcccgtcgg caaacacgta aaacgcgccg gtgggttcaa catggatcac
     5941 aaaacccata tccgtcagac gcgccagcat gtagcggcgt cgttcgtcgt aggtccggat
     6001 catggtccgg acggccgtct ttgtctttgc aagcgccgcg attcccgccc actgaatgaa
     6061 gccgcccgcc gaaatcatga aattctgatg aatgcgctgc atgaccgcgc tgaatttttt
     6121 gggaaagatc aggtagccca gacgccagcc ggtcatcgcg tagagcttgg aaaagccgtt
     6181 gatgacaaaa gcccggtcgg taaactccag aatggagtgc gcccggtccc gatagacgag
     6241 cccgtggtaa atttcatcgg aaataatata gggcgcgtca aaccgggcga tgtcctgcag
     6301 ctgttccctg ctcatgacaa tgccggtcgg attgcacgga gaattgatca tgatgccttt
     6361 cgtccgacgg gagagttttt tggcgatgtc ctgatgccgg tattgaaatc cttcttccgg
     6421 gtaggtttga atttccctga gcctgccgcc cgccgccaga atgaaattct gatagcaggg
     6481 gtagcgcggg ttggaaacaa tgacttcatc gccgtgatcc agcagcgcga gcatggcaaa
     6541 cagcaggacc ggcgatgttc cggtggagat gaggacctgg tcgggcgaaa tggatacgcc
     6601 gtactcccgc agatagaaat cgcagatcgc ctgccgcagt tcaagaagcc ccagggcgtg
     6661 tgtgtaacgg gttttgttgt ttttgagcgc aacgatggcc gcgtcgatga ctgctttcgg
     6721 cgtcggaaaa tccggctcgc ccacttccat gtggatgatg tgttcgcctt ttcgttccat
     6781 ctcttcggct ttggccatga tatccatgac gataaaaggc gtgaacccgg atgctcgttt
     6841 tgaaatcata caatcctttt tcttcagtat tcgcagggaa ccgtcgcgac ggttttctcc
     6901 ctctctatgg tgattgtctg aatttttcaa ctgccttttt gcggcgggcg ctggaaaccg
     6961 ttgttccgga aggatgggtt tccgcctttt gccgtggccg ccggtgatgc attattctcc
     7021 acccggtggg gagatatgcc cctttgcgcc tccgccagga aaaaggattt tggcgcctgg
     7081 aaaaaacctt ccattatttc gccccgatag ccgtcgcgcc ttttttctta aaatggcatg
     7141 ttcgttgctg tatcgcgggc aacaaagagg ctctgaaaaa gggcttacta aaaagattac
     7201 aaggaggaat ttcagatgaa gcgtttggta cttttagttg ttgcgtttgt gtttgcattg
     7261 agcatgaccg cggtagccgc cgacaaggcc gcccccgcag ccgcccccgc cgacaaagcg
     7321 gtagccgccg acaaggcgac tccggatgcc gacaagaagg ctgatgtgaa gaagcctgca
     7381 aagaaagcaa aaaaagcaaa aaaagcaaag aaggccgaaa agaaggctga agaagccgcc
     7441 cccgcggcac cggccgctcc cgcagcgaag taaactttcc agcggaagga acacgaaaaa
     7501 gggacgggtg ttcacccgcc cctttttttt gttccaacag attttatttt gcgttcagcc
     7561 gtcaagacgc tttgctcgcg gcacccgttt cagcggcggc atattccgcc agcgcttcca
     7621 gcagctttac ctttttgatc ggcttggtca tatgtccggt gcatcccgcg tcgagacttt
     7681 tcttttcgtc ctctttcagc gcgtacgcgg ttaaggcaat aatgggaacg gctgtctttc
     7741 cctgggattt ttcccatttc cggatctcgc gggtggccgc atagccgtcc atcacgggca
     7801 tttgcatatc cattaaaaca atgtcatatt tcgcggactt gaaggcttcg acggcgatgg
     7861 cgccgttttc ggccatctga agccggtgcg gcgtattttt caggtaggtc gaaatcagga
     7921 gccggttgtc ttcgttgtct tccacgagaa gaatgttcaa ggatttttgc gcacccgaag
     7981 cagcaggcgc tgtttcggct tgtggctgcg ccgcctcttt ctttcccagc tctccgcaga
     8041 tggcttcctg catatcattg cgcttgacgg gtttatagag aaaccgctcg atgcccactt
     8101 ttttcgccgc cgcgatgtcc tcctgcggat agccggagga tagcatgatg atggccagcg
     8161 acgaccactt cggctccttg cggatttcca ccgccgtcat cagtccgtcc attccgggca
     8221 tctgataatc gaggatgaca aaatcatacg gcttgccggc ttcctgcgct tcccgaagcg
     8281 cggtcaggcc ggccggcccg ctgttcgcgg aagagacgac gatttcccat tgcgtcagca
     8341 tctcccggag aatcagacga ttgttggcgt tgtcatcgat tacgagggcg cggcgccccg
     8401 tcaaatcgac cggcgcgcag gcgtcttccg cgggcgtcat ctccgtctgc atcgcaaaag
     8461 gcgcggtaaa ggaaaaaatg ctcccccttc cctctttgct ttccactgaa atcgttccgc
     8521 ccatcagatc tacgatggat ttggaaatgg tcaggcccag ccccgttccg ccgtatttgc
     8581 gagtggtgga agaatcggcc tgcgtaaacc gctcaaagat gttgtggatt ttatccgctg
     8641 gaatcccgat gcccgtatcc t
//
LOCUS       DAOSRM010000091         8287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_8167_length_8287_cov_107.062439, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000091 DAOSRM010000000
VERSION     DAOSRM010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8167_length_8287_cov_107.062439"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1391
                     /locus_tag="PLA58_08550"
     CDS             183..1391
                     /locus_tag="PLA58_08550"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOR62882.1"
                     /translation="MAKKIKKVVLAYSGGLDTSVIVRWLIETYGCEVICFAADVGQKE
                     ELTGLKQKAIRTGASKIYIEDLREEFARDFVFPALRANAIYEGTYLLGTSLARPLIAK
                     RQIEIAQKEKADSVCHGATGKGNDQVRFELTYIALNPKINIIAAWKDDHWTFDSRESM
                     FDYAEKHNIPLPLTKSKPYSSDRNLLHISHEGAILEDTWAEPPEDIFVLTRSPEAAPD
                     KPAYVEIDFVKGNPVAIDGKKMSPAKLMDRINLLAGANGVGRVDMVENRFVGMKSRGV
                     YETPGGTVLWAAHRAVESITMDREVMLMRDSLIPKYAQLAYNGFWYAPEMQTLQAYID
                     ATQENVTGTARMKLYKGNCIVVGRKSPYSLYSEAFATFEKDEVYNQKDAIGFIRLNGL
                     RLRIKKMMKK"
     gene            1415..2803
                     /gene="argH"
                     /locus_tag="PLA58_08555"
     CDS             1415..2803
                     /gene="argH"
                     /locus_tag="PLA58_08555"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOR62883.1"
                     /translation="MADAKKPWAGRFQEATAPSVERFTSSLHVDRRLYRHDIEGSIAH
                     ATMLADSGIISRADAKAIIRGLKSIRADMEKGRFVFDPADEDIHMAIEKELIRRAGDA
                     GAKLHTARSRNDQIALDVRLYLRDEADGLIRLLNALQKQLVGMAKKEIATIMPGYTHL
                     QKAQPVLLSHYLLAFAEMFARDGERLEDCRKRLNVLPLGAAALAGTGLPIDRTRTARL
                     LRFPEVSRNSMDTVADRDYIAEFIFAASLAMAHLSRFCEDLVLWSSEEFGFAEISDAY
                     TTGSSIMPQKKNPDVAELIRGKTARVYGDLTAILTLVKGLPMTYNRDLQEDKEPLFDA
                     VDTLHDCLAVFLEMLAHTRFNAARMHLAARGGFSTATDVAEYLVEKGVPFRSAHEIAG
                     GIVAYCIKTKRTLEDLTLGEYQSFYGGFEASILSRIRLENAVNARRHFGGTAQKAVRE
                     RIREFEKILDKK"
     gene            2778..3212
                     /locus_tag="PLA58_08560"
     CDS             2778..3212
                     /locus_tag="PLA58_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62884.1"
                     /translation="MKKFLIKNSIGPAALCALLLLGCGLKANPVAPASGAARGGTVQK
                     PSVAAEGGAAVLSWRLSSSDRVSYTIIERSTLGSAGNICRDCPRTFEPIDRLPADNAG
                     EKSREYRFVDSSVGKGKTYSYRLQLCEETGECRESQTVEIDF"
     gene            3240..4115
                     /gene="dapA"
                     /locus_tag="PLA58_08565"
     CDS             3240..4115
                     /gene="dapA"
                     /locus_tag="PLA58_08565"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOR62885.1"
                     /translation="MFRGAITALVTPFKNEKVDEAALRDLIEFQIANGIDGLVPCGTT
                     GESPTLSHDEHDRVIEITIDAAKKRVPVIAGTGSNSTAEALRLTRHAYEAGADGALIA
                     CPYYNKPTQEGLYRHFSQIAKSVPFPIVPYNIPGRTGVNMSPELIARLAKIDNIVGIK
                     EASGSLKQMNDVLDLCGPEFDVLSGDDGFTLPLMAIGGKGIISVASNIVPADMAAMVD
                     AAEAGDLAAARALHKKMSPLFDVLFIEVNPIPVKAALALMGRIAGEYRLPLCPMAPAN
                     LEKLRKALADYGLIS"
     gene            4171..4983
                     /gene="dapB"
                     /locus_tag="PLA58_08570"
     CDS             4171..4983
                     /gene="dapB"
                     /locus_tag="PLA58_08570"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOR62886.1"
                     /translation="MVKVIVTGAGGKMGSRIIGMISAMEELRVVGAIEVKGHPIIGRD
                     LGHSLGAGRTGALVSDSLADCIDQADVVIDFTDHEASLTYLKIASEKNRAIVIGSTGF
                     TAEEMATARRLAEKTRCVLSPNMSVGVNVMLKVLEYCAGILYEDYDVEIVEAHHHLKK
                     DAPSGTAMQMARVIAGRLGRDLDKEAVYERRGMIGERTKREIGIQTIRAGDIVGEHTV
                     IFGGIGERLEFIHRAQSRDNFAKGAIRAAQWIVKQKNGLYDMQDVLGLRDSR"
     gene            4993..5517
                     /gene="folK"
                     /locus_tag="PLA58_08575"
     CDS             4993..5517
                     /gene="folK"
                     /locus_tag="PLA58_08575"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOR62887.1"
                     /translation="MLEGTLAYIGIGANLDDPPAQCRQAVERLSGVSGITLERVSSFY
                     RTEPVIPESAGRKQRGELEKQNWFVNAAAEIRTVLSPRDLLKELQHIENSMGRVRTIK
                     GGPRIIDLDLLLYGQEIHEEEGLTVPHPEMHRRLFVLEPLCEIASYLIHPAFGVSMRG
                     LKDRLDDNKTVERI"
     gene            5535..5795
                     /locus_tag="PLA58_08580"
     CDS             5535..5795
                     /locus_tag="PLA58_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62888.1"
                     /translation="MNIIKVLAILVLIYVGYRIVTMFRRMKSQEARAYRVDDAPKGED
                     LVQDPFCKTYVPKSQAYVKEIDGKPHYFCGKECFEKYLTEKK"
     gene            5820..6467
                     /gene="fsa"
                     /locus_tag="PLA58_08585"
     CDS             5820..6467
                     /gene="fsa"
                     /locus_tag="PLA58_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOR62889.1"
                     /translation="MKFFIDTANIAEIREGLSLGMVDGVTTNPSLIAKEKRGFDAVVK
                     EILEIVEGPVSLEVVSPEAKGMVAEGKKLARLGDQVVIKVPMTTEGLKATKLLAADGI
                     DVNQTLVFSPLQALMAAKAGAAYVSPFVGRLDDIAHDGMELAQQILDIYDNYEYDTEV
                     IVASIRHPKHVLDAALMGADIATIPFKVIAQLAQHPLTDKGIALFLEDWKKVPEK"
     gene            6532..7104
                     /gene="pgsA"
                     /locus_tag="PLA58_08590"
     CDS             6532..7104
                     /gene="pgsA"
                     /locus_tag="PLA58_08590"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOR62890.1"
                     /translation="MNKRREIWNLPNSITLARISVVPFLFFLLASPGPFWSLTLAALF
                     VLAALTDFLDGYIARKYNLITTMGKFLDPLADKLIVNTAMILMIPIGRIDAWIVVVII
                     IRDLIVDGIRSISSSEGIYIQASVLGKQKTVAQIIAVTALMIHYPFLGLDAHLVGTAI
                     LYAALLLTVYSGLDYLFQFYRGTYMRPENH"
     gene            7237..7311
                     /locus_tag="PLA58_08595"
     tRNA            7237..7311
                     /locus_tag="PLA58_08595"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7269..7271,aa:Gly,seq:gcc)
     gene            7320..7395
                     /locus_tag="PLA58_08600"
     tRNA            7320..7395
                     /locus_tag="PLA58_08600"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7353..7355,aa:Cys,seq:gca)
BASE COUNT         1948 a         2104 c         2472 g         1763 t
ORIGIN      
        1 ttttttgtca tttcgaccga agggagaaat ccatgtcccg aatgcaatga gggatcttta
       61 gatttctcgt cgctcacgct cctcgaaatg acatttttca aagctctcga aaaaagggat
      121 aaaacatttt gaaccggaag acccggcaaa tgaaagaaaa gatgttttga ggaggaaaaa
      181 tcgtggcaaa aaaaattaaa aaagtggtgt tggcatattc cggcggtctg gacacgtcgg
      241 tgatcgtgag atggttgatt gaaacctacg gctgcgaggt catttgcttt gcggccgacg
      301 tggggcagaa ggaagagctc acagggctta agcagaaagc catccggaca ggcgccagca
      361 aaatctacat cgaagacctg cgggaagaat ttgcgcggga tttcgtgttt ccggcgctgc
      421 gcgccaacgc gatttacgag ggaacctatc tgctcggaac atcgctggcc cgtcccctga
      481 tcgccaaacg gcagatcgaa atcgcccaaa aggaaaaggc ggactcggtt tgccacggcg
      541 cgaccggaaa gggcaacgat caggtgcgct ttgagctcac ctacatcgcg ctcaacccca
      601 aaatcaatat cattgcggcc tggaaggacg accactggac gttcgattcc cgggagtcca
      661 tgtttgatta cgcggagaaa cacaacatcc cgcttccgct gaccaagtcc aagccctaca
      721 gctccgaccg caatctgctg cacatttccc atgaaggcgc cattctggaa gacacatggg
      781 cggagccgcc ggaagacatt ttcgtgctga cccggtcgcc cgaagcagcg ccggacaagc
      841 ccgcctatgt ggaaattgat tttgtcaaag gcaaccccgt ggcgatcgac ggcaaaaaga
      901 tgtcgcccgc gaagctgatg gaccgtatca atttgctggc cggagccaac ggcgtcggcc
      961 gggtggacat ggtggagaac cggtttgtcg gcatgaaatc ccgcggtgtg tatgaaacgc
     1021 ccggcggcac ggttctgtgg gccgcgcacc gggcggtgga gtccattacg atggaccggg
     1081 aggttatgct gatgcgcgat tcgctcattc cgaagtatgc ccagctcgcc tacaacggat
     1141 tctggtatgc gccggaaatg cagacgcttc aggcctacat cgacgcgacg caggaaaacg
     1201 tcaccggcac ggccaggatg aaactctaca aaggcaactg catcgtggtc ggccgcaaat
     1261 ctccctattc gctctacagc gaggcgtttg ccacgtttga gaaagacgaa gtctataacc
     1321 agaaggacgc catcggcttc atccgcctca acggcctgcg gctcaggatc aagaaaatga
     1381 tgaaaaaata aaccatctca aaagagggcg catcatggct gacgcaaaaa aaccctgggc
     1441 gggacgattt caggaagcca cggctcccag cgtggagcgc tttaccagct cccttcatgt
     1501 tgaccgccgc ctgtatcgtc atgacattga aggttcgatt gcccatgcga cgatgctggc
     1561 tgattcgggc atcatttccc gcgcagacgc gaaggcgatc atccgcgggc tgaaaagcat
     1621 ccgggccgat atggaaaagg gtcgttttgt tttcgacccc gccgacgaag acattcacat
     1681 ggccattgaa aaagagctga tccggcgcgc cggagacgcg ggcgcaaaac tgcataccgc
     1741 gcgcagccgc aacgatcaga tcgcgctgga tgttcggctg tacctgcgcg acgaggcgga
     1801 cgggttgatc cgcctgctca atgcgctcca gaagcaactg gtcggcatgg ccaaaaagga
     1861 aatcgccacc atcatgcccg gctacacgca tctgcaaaag gcgcagccgg ttcttttgtc
     1921 tcactatctg ctggcgtttg ccgaaatgtt tgcccgcgac ggagagaggc tggaagactg
     1981 ccgaaagcgc ctgaacgttt tgccgctggg ggcggcggcc ctggccggga cggggctgcc
     2041 gatcgaccgg acccgcacgg cccggcttct tcgctttccg gaagtcagcc gcaacagcat
     2101 ggatacggtg gccgaccggg actacatcgc ggagtttatt tttgcggcgt cgctggccat
     2161 ggcgcacctg agccgtttct gcgaggatct ggtgttgtgg tcttccgaag agttcggctt
     2221 tgccgagatt tccgatgcct acaccaccgg cagcagcatc atgccgcaga agaaaaatcc
     2281 ggacgtcgcg gagctgatcc ggggcaaaac ggcaagggtt tacggcgatt tgacggcgat
     2341 tctgacgctc gtcaaagggc ttcccatgac gtataaccgg gatcttcagg aggacaagga
     2401 gccgttgttt gacgcggtgg acacgcttca cgattgcctc gcggttttcc tggaaatgct
     2461 ggcacacacc cggtttaacg ccgccagaat gcatttggcg gcccgcggag gtttttcaac
     2521 cgccaccgat gtggcggaat atctcgttga aaagggagtc cccttccgca gcgcccatga
     2581 aatcgcgggc ggaatcgtcg cctactgcat caaaaccaaa aggacgctgg aggacctgac
     2641 gctgggagag tatcagtctt tttacggcgg ctttgaggcg tcgatcctct ccaggatacg
     2701 gctggaaaac gccgtcaacg cccgtcgtca ttttggcggc acggcgcaaa aggcggttcg
     2761 ggaaaggatt cgggaatttg aaaaaattct tgataaaaaa tagcatcgga ccggctgcgc
     2821 tttgcgctct tttgcttctg ggctgcggcc tgaaggccaa tcccgttgcc ccggcgtccg
     2881 gcgctgcccg gggcggaacg gttcagaaac cgtcagttgc agccgaagga ggcgctgcgg
     2941 ttttgagctg gcggctttca tcttcagacc gggtcagtta cacgatcatc gagagaagca
     3001 cgctgggaag cgcggggaac atttgccggg attgtccgcg cacgttcgaa ccgatcgacc
     3061 ggctgccggc ggacaacgcc ggggaaaaga gccgggagta tcggtttgtc gattcatcgg
     3121 tcgggaaagg aaagacatac agctaccgct tgcagttgtg cgaggaaacc ggcgaatgcc
     3181 gggaatcgca aaccgtggaa atcgattttt aaataagaaa tccggaggag gcctgtttta
     3241 tgttcagagg agccattacc gcgctggtga cgccttttaa aaatgaaaag gtggatgagg
     3301 cggcgttgag ggatctgata gagttccaga tcgcgaacgg catcgatggt ctggtgccct
     3361 gcgggacgac cggcgaatcg ccgaccctgt cccacgatga gcacgaccgg gtgattgaaa
     3421 tcaccattga cgcggcgaaa aaacgggtgc cggtcattgc cgggacggga tccaacagta
     3481 cggcggaagc gctgcgcctg acccggcatg cgtatgaggc gggcgccgac ggcgcgctca
     3541 tcgcctgtcc ctactacaac aagccgacgc aggaagggct ctaccggcat ttttcacaga
     3601 tcgcgaagag cgtgcctttc ccgatcgttc catacaatat tcccgggagg accggcgtaa
     3661 acatgtcgcc ggaattgatt gcccgtctgg ccaaaatcga caacatcgtc ggaatcaagg
     3721 aagcgtccgg atctctcaag cagatgaatg acgtgctgga tctgtgcggg ccggagttcg
     3781 acgtattgtc cggcgacgac gggtttacgc ttcccctgat ggcgatcggc gggaagggga
     3841 tcatttccgt cgcctccaat atcgttcccg ccgatatggc cgcgatggtc gatgccgccg
     3901 aagccgggga tctcgctgcg gcgcgggcgc tgcacaaaaa aatgagtcct ctgtttgatg
     3961 ttctgttcat tgaggtgaac ccgattcccg tcaaagccgc tctggcgttg atgggcagga
     4021 ttgccggtga ataccggctg ccgctttgcc cgatggcgcc ggcgaattta gagaaactca
     4081 gaaaggcgct ggccgattac ggtctgattt cctagggcga cagggaaggt tttattcgaa
     4141 gttacaggaa tgaaagaagg agcaagcagt atggtaaagg taattgtaac aggcgccggc
     4201 gggaaaatgg gaagccggat catcggcatg atttcggcca tggaagaact tcgcgttgtc
     4261 ggggccatcg aggtcaaggg ccatccgatt atcggaagag atttggggca ttctctgggg
     4321 gcggggcgaa ccggcgcgct ggtcagcgac agtctggccg actgcatcga tcaggccgac
     4381 gtcgtgatcg actttacgga ccatgaagcc tcgctgacct atctgaagat cgccagcgaa
     4441 aaaaaccgcg ccatcgtgat cggctccacg ggcttcaccg ccgaagaaat ggcaaccgcg
     4501 agaaggcttg ccgaaaaaac acgctgcgta ttgtcgccga acatgagcgt gggcgtcaat
     4561 gtgatgctga aggtgctgga atactgcgcc ggcattcttt atgaagatta cgatgtcgaa
     4621 atcgtcgagg cgcatcatca tctgaaaaag gacgcgccca gcggaacggc catgcagatg
     4681 gcgcgggtga ttgccggcag gctggggcgt gatctggata aagaagcggt gtatgagcgc
     4741 cgcggcatga tcggagagag gaccaaaaga gagatcggca ttcagacgat ccgggccggg
     4801 gacattgtgg gcgagcacac ggtgattttc gggggaatcg gggagagact ggaatttatc
     4861 caccgcgcgc agagccggga caattttgcc aagggagcga tccgcgcggc gcaatggatt
     4921 gtcaaacaaa aaaacggcct gtatgacatg caggacgttc tggggctgcg ggactcccgc
     4981 tagggaggcg ttatgctgga gggaaccctc gcctatatcg gcatcggcgc caatctcgat
     5041 gaccctccgg cgcagtgtcg tcaggccgtc gaaagacttt ccggcgtttc ggggattacg
     5101 ctggaaaggg tctcatcctt ttacaggaca gagccggtga ttccggaatc ggccggccgg
     5161 aaacaacggg gggagctgga gaagcagaac tggtttgtca acgcggcggc ggaaatccgg
     5221 accgtccttt cgccccggga ccttttgaag gaactccagc atattgaaaa ttccatgggg
     5281 cgggtccgga cgattaaggg aggacccaga attatcgacc tggacctgtt gctgtacggc
     5341 caggagattc atgaggaaga ggggctgacg gtgccgcatc cggaaatgca ccggaggctt
     5401 tttgtgctgg aacccctgtg cgagattgct tcctatttga ttcacccggc attcggcgtt
     5461 tcgatgcggg gactgaaaga ccggctggac gacaacaaaa ccgttgaacg catttgaagg
     5521 aaaagaaggt tgacatgaat atcatcaaag tgctggctat cctggtattg atttacgtgg
     5581 gttacaggat tgtaaccatg ttccgaagga tgaaatcgca ggaggccaga gcctaccgcg
     5641 tggacgacgc gccgaaaggg gaggacctgg tgcaggatcc gttttgcaaa acctacgttc
     5701 ccaaaagcca ggcgtacgtg aaggaaatcg acggcaagcc gcactacttt tgcggcaaag
     5761 aatgctttga gaagtacctc acagagaaaa aataacgcat agaaacttca ggggggatta
     5821 tgaaattttt tatcgatacg gcgaacattg cggaaattcg ggaaggactg tcgctgggaa
     5881 tggtggacgg cgtgacaacg aatccgtctc tgattgccaa ggaaaaaagg ggctttgatg
     5941 ccgtggtgaa ggaaatcctg gaaatcgtgg aggggccggt cagcctggag gtggtgagtc
     6001 cggaagcgaa agggatggtt gccgaaggca aaaaactggc ccggctgggc gatcaggtgg
     6061 tcatcaaggt gccgatgacg accgaaggcc tcaaagcgac caagctgtta gccgcggacg
     6121 gcatcgacgt caatcagacc ctggtttttt ccccgctgca agccctgatg gcggccaaag
     6181 ccggcgcggc ctatgtcagc ccgtttgtcg gacgtctgga cgatatcgcg catgacggca
     6241 tggaactggc gcagcagatc ctggacatct acgacaacta tgagtatgac accgaggtca
     6301 ttgtcgccag tatccgtcat ccgaaacatg tgctggacgc cgcgctgatg ggcgcggaca
     6361 ttgccacgat tccttttaaa gtcatcgcgc agcttgccca gcatcccctg acggacaagg
     6421 ggattgcgct gtttctggaa gactggaaaa aagtgccgga aaaatagtcg gatcgctgcg
     6481 ggaaatgaga cggtgaatcg gcccgaatgg gtaaaacagg cgaaagggag tatgaacaaa
     6541 agacgagaaa tatggaatct tcccaacagc attacgctgg cccgcatcag cgttgtgccg
     6601 tttttgttct ttctcctggc gtcccccggc cccttctggt cgcttacact ggccgccctg
     6661 tttgttctgg ccgcgctgac ggattttctg gacgggtata tcgcccgcaa atataatctg
     6721 atcaccacaa tgggcaagtt tctggatccg ctggccgaca agctgattgt caacacggcc
     6781 atgattctta tgattccgat cgggcgaatt gacgcctgga ttgtcgtcgt catcatcatc
     6841 cgggacctga tcgtggacgg catccgcagc atctcgtctt ccgagggaat ttatatccag
     6901 gccagcgtcc tgggaaagca aaaaaccgtc gcccagatca ttgcggtgac cgcgctgatg
     6961 atccattacc cctttttagg gctggatgcg catctggtcg gcacggccat tctttacgcg
     7021 gctttgctgc tgacggttta ttcggggctg gattatctgt ttcaattcta tcggggaacc
     7081 tacatgcggc cggagaacca ttgatattat tggattccgg tttcggcgtc atggtccggc
     7141 agaaagcagg gcggcgtttg aagaggcgca gggaacgttc atttttctat tgacaagctg
     7201 aaacgatttg ttatattcac ccgcgttctt aagtgagcgg gcgtaactca gtggtagagt
     7261 gctaccttgc caaggtagac gtcgacggtt caaatccgtt cgcccgctcc attttttttg
     7321 gcggcatagc caagtggcta aggcagcggt ctgcaaaacc gttattcatg agttcgaatc
     7381 tcattgccgc ctccatgaaa atcaatagct gcgggattga tccccgcagc tattttcgtt
     7441 ttctccggga ttatgacaca gtctattttt catcattccc gcaaagatac tatgttgcaa
     7501 ttcattttca agtcgtttcg ctcttctctg gaaagagtga gtcccatgta gtgcgtagct
     7561 ttagctgcgc tttaacgggt ctgaagaccc gtgctatatt catttagaat gaaaaagggg
     7621 atgttattgc atgctctccc gggtgatctt ccggtttcca ttcagtttgc tcaattccag
     7681 attttgacgg cttcgtaaaa agtcaatttc gcactctttt cgtcataccg gcaaaggccg
     7741 gtatccagta attacaatga gttatggacc ccggctttcg ccggggtgac ggtgtgggtt
     7801 gctttttacg aggtcgtcaa agtttccgca tttttttgtc cacggcaacc acgtatccgg
     7861 aagagatgga gacgatggaa tcggggattt tttcagcagc gtggaaccgg ctcgttggaa
     7921 aagcggcgga aataaaaagc atcagaaggg acagggcaag aaggggggcg cattcaatct
     7981 ggagaacata tgtttgtaga gagctttgaa aaatgtcatt tcgaggagcg tgagcgacga
     8041 gaaatctaaa gatccctcat tgcattcggg acatggattt ctcccttcgg tcgaaatgac
     8101 aaaaacggcc gttaatgaac aaccccgccg caagcagcgg ggtatcaaaa gctttctatt
     8161 ttgtatgggc cattcgccgc aagcggcggg gaatggaacc ctaagagatt caaagctctc
     8221 ttgtcttatg ctgtatttgt gaggcgttgc tttttaacaa aatccgatag gcgtttcaag
     8281 ttctttc
//
LOCUS       DAOSRM010000092         7961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_8566_length_7961_cov_111.937895, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000092 DAOSRM010000000
VERSION     DAOSRM010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7961
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8566_length_7961_cov_111.937895"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLA58_08605"
     CDS             complement(<1..750)
                     /locus_tag="PLA58_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR62891.1"
                     /translation="MTPEKKLRIGVVGIGHLGGYHLQKYARIGSCVIAAVSDSQIEKA
                     QKAADLYGCPAFADHRDMLDRVDAVSIAVPTGDHFAVARDFLAAGIDALIEKPLCASL
                     EEADELLALARKNRLILQVGFVERFNPAVMALEQVITRPAFIEVHRLHPFFERGTDVD
                     VILDLMIHDLDLILKFVDAPVTRVEAVGVPILSDKIDISNVRLSFATGCIANITASRI
                     SAKTMQKIRFFGPEGYHSVDCRKREVLSFSKI"
     gene            complement(761..1534)
                     /gene="lpxA"
                     /locus_tag="PLA58_08610"
     CDS             complement(761..1534)
                     /gene="lpxA"
                     /locus_tag="PLA58_08610"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOR62892.1"
                     /translation="MKIHPTAIVAPDAQLAEGVEIGPYSIIGSDVVIGKNTVIGPHTV
                     IDDYTRIGEGCRIFQFCSIGAPPQDLKFGGEKTRVVIGNFNTIREFVTIHRSTSADIG
                     VTILGDHNLIMAYCHVAHNCKLGNHIIMSNVATLAGHIHVEDYAIISGLSGVHQFTRI
                     GAHSFIGGASGVARDVPPYTLANGNHAKLFGLNLIGLQRRNFSEQTIKALKDAYKIVF
                     RSDLLLEAALDKAQKEVEDLPEVRHFLAFIKNSERGVCR"
     gene            complement(1531..2580)
                     /gene="lpxD"
                     /locus_tag="PLA58_08615"
     CDS             complement(1531..2580)
                     /gene="lpxD"
                     /locus_tag="PLA58_08615"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOR62893.1"
                     /translation="MKKSIAEIAALVGGAVAGDGQKLIGDIRPIEEAGADDLTFVSNP
                     KYFKWLKTTLAGAILVPPGTVAPDKNLIAVADPYAAFGKLLAVFYPVDHGPAGISPDA
                     YIEEGASVSPEATVMPRAFISRGARVDKGAVLYPGVFIGRDASVGEDSVLYANVSVYA
                     RSVIGRRVILHSGVVIGGDGFGFSAPGSQNKKIPQVGVVQIDDDVELGANTTVDRATL
                     GKTWIGRNVKIDNLVQIAHNVVIGENSAIAAQTGISGSTKIGKSVIIGGQVGIVGHIT
                     IGDSVMIGASSGIHKSIPAGMVGGGTPFLPFREWLKVEVSKAKLPEIRAKMEKLLRRV
                     EELEAVIHKNREGEP"
     gene            complement(2596..3126)
                     /locus_tag="PLA58_08620"
     CDS             complement(2596..3126)
                     /locus_tag="PLA58_08620"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOR62894.1"
                     /translation="MKKNMLLIAGLTLLLFAWTNTALAADKIGFINMQTIIQNSNAGK
                     KAAEDFKKLFARKQENIKSMENEVKKLKDELDKQGAVMTASARGDKETAYQRKMRDYQ
                     ILVDDTNKELQKRDQEYSQQLIPEILKVVRAIAEKEKYTVIMDISTMPLPYFDKAGDI
                     SKKVIDDFNKTSGAKK"
     assembly_gap    3187..3286
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3291..5936)
                     /gene="bamA"
                     /locus_tag="PLA58_08625"
     CDS             complement(3291..5936)
                     /gene="bamA"
                     /locus_tag="PLA58_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOR62895.1"
                     /translation="MIFNFKTIRLFIAVLLLLFAAEARADGPKKICVLPFEVYALSGS
                     AELQASVYNSLLTEFGREKKVEVKTAGGFEPGSPVPNRQEADAAGKALGADYVVTGSI
                     MQFGEALNIDAQIIDVAEGKILPAVSVQGKGDAGARALAAALKREILDRAGLLDKIAR
                     VEIAGNRKIGADAIRQKIKSRPGNPLDEEDISDDIKTIFKMGYFLDVSADVATEAAGK
                     VLTFNVVEKGLISEIRLIGNKKVDKDDILEAMSVKVRQSVNPENIKQDLQKIKALYDT
                     KGYYNAEITDRLEKDGEKDFVLILDIKENDRVYIRSITFEGNESFTTKELVGMMATNE
                     RTLLGFITDTGILQSQQLKQDVQRLTAFYFNNGFVNAQIGEPVITHDDKGIYIKIVVR
                     EGKRFKIGKVEISGDYLEKTRADLYAQLKTKSGSHYSREAIMKDMEMITTTANDEGYA
                     NADVNPQVTTHEKEQLVDLNFLLKKGELVHIARIGISGNTTTRDKVIRRSLFIVEGDL
                     YSSTKLKKSYENLNQLRYFEEVDIQTEKAPDNQMDINIRVKEKNTGMFMIGAGYSAVD
                     QAVIMAQIVQQNFLGYGQTMSLKASLGSKTNNYELSFTEPFLFDLPLWFKADVWKYKK
                     EYDSYTLDTMGAGVTLGHPLFWKIVGYVGYKYSIDNIQDINYATASYYIKVQEGERKT
                     SAVTFTMSYDTTNDVMFPSKGLKSTASVQYAGMPLGGNTKFVKYNASTIGFVPLFWDM
                     VFSLKGQIGYLQNNDDSDDKIPIYERYVLGGINSLRGLRYVGPTNPGTSDVVGGTTML
                     TGTAEVVFPLVKDAGIKGVVFYDTGNTWNGGYYLDDLRSTCGAGIRWYSPIGPLRLEY
                     GYVLDNKGLSDDSKGRWEFTIGMFM"
     gene            complement(5926..6594)
                     /locus_tag="PLA58_08630"
     CDS             complement(5926..6594)
                     /locus_tag="PLA58_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR62896.1"
                     /translation="MIKLENLSKTFVKDGNKIEVLKGLNLEIARGDSLAVVGVSGAGK
                     STLIHILGTLDHPTGGSLSIAGQTVFNWNEKKIAAFRNRTIGFVFQFNNLLPEFTALE
                     NAMMPAFIGGLERKKALDRAAHLLTEVGLEHRLRHKPGELSGGEQQRVAIARALAMEP
                     EILLADEPTGNLDTETGKKIEDLLIALNAEKKITLVVVTHNRLLAERMSGRIGLRDGK
                     IYDF"
     gene            complement(6616..>7961)
                     /locus_tag="PLA58_08635"
     CDS             complement(6616..>7961)
                     /locus_tag="PLA58_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HOR62897.1"
                     /translation="AKKTFYEAVKINGFPTGQCLMPYEFFISRRYMRAKRKQVFVSII
                     TFISVFGIFLGVAALIIVLAVMNGFEEDLRSKILGIRSHIEVTADMSPMKNYPDVRRE
                     IDRIPGVVASTPFIYTEAMIRSQNGVTGVIIRGMDTESAPRVINLGKIRQGSVGHLDR
                     IPEAVRRKLPAADRELAGILIGREMARNLGIFLHDPITIISPAGGIATPMGMVPRMKK
                     FVVVGIYESGFYEYDSKLAFLSLTSCQDFLEMGDAVSGIDIVVRDIYKADVIARQIQN
                     RLGFPFYAQNWMQMNKNLFSALKLEKRVMFIILSLIVLVAAFNIISALIMIVMEKNKD
                     IAILKSMGATSANIMKIFIYQGLIVGVVGTFFGCIAGLAVALNLQKISLFVERVFNFK
                     ILPGDVYYLSELPSKVNYGDVAVIVAGTLVICFLSTIYPSWRASRLDPAEALRYE"
BASE COUNT         1493 a         2210 c         2254 g         1904 t
ORIGIN      
        1 gattttggaa aacgacagaa cctctctttt ccggcagtcc acggagtgat agccttccgg
       61 gccgaaaaac cggatcttct gcatggtctt ggcgctgatg cggctggccg tgatgttggc
      121 gatgcagccg gtggcaaacg acaggcggac gttggagatg tcgatcttgt ccgaaagaat
      181 gggaacgccg accgcctcca cgcgggtgac cggcgcatcc acgaatttca ggatcaggtc
      241 caggtcatga atcatcagat ccagaatgac gtccacgtcg gtgccgcgct cgaaaaaggg
      301 atgcaggcga tgcacttcaa taaaggcggg ccgggtgatc acctgctcga gggccatcac
      361 ggcgggattg aaccgttcga cgaatccgac ctgcagaatc aggcggtttt tccgcgccaa
      421 tgccagaagc tcgtcggcct cctcaagcga cgcgcacagc ggcttttcga tgagcgcgtc
      481 aatgccggcg gccaggaaat cccgggcgac ggcaaaatga tctcccgtcg gcacggcgat
      541 gctgaccgcg tccacgcgat ccagcatgtc ccggtggtcc gcaaaggccg gacacccgta
      601 aagatcggct gccttctgcg ccttttcgat ttggctgtcg gacacggcgg caatgacgca
      661 ggatccgatc cgggcgtatt tctgaagatg ataaccgccc agatggccga tcccgaccac
      721 gccgatgcgt aatttctttt ctggggtcat gttttatctc tcagcggcag acgcctcttt
      781 ccgaattttt gatgaaggcg agaaaatgcc ggacctccgg gagatcctcc acctcttttt
      841 gcgctttatc cagggcggcc tccagaagca gatcggagcg gaaaacaatc ttgtaggcgt
      901 ccttgagcgc tttgatggtt tgttcagaaa aattgcgccg ctgcaggccg atcagattga
      961 ggccgaacag tttggcgtgg ttgccgttgg ccagcgtgta ggggggaaca tcccgggcca
     1021 cgccggaagc gccgccgatg aacgaatgcg ccccgatgcg cgtgaactga tggacgcccg
     1081 acaggccgct gatgatggcg tagtcttcca cgtgaatgtg gccggccagc gtggcgacgt
     1141 tggacatgat gatatggttt cccagcttgc agttgtgcgc gacatggcag tacgccatga
     1201 tcagattgtg gtcgccgagg atggtgacgc cgatgtcggc ggacgtcgag cggtggatgg
     1261 tgacgaattc gcggatggtg ttgaagttgc cgatgaccac ccgcgtcttt tcaccgccga
     1321 atttcaaatc ctgcgggggc gcgccgatcg agcagaattg aaagatgcgg cagccttcgc
     1381 cgatgcgggt gtaatcgtca atgacggtgt gcggaccgat cacggtgttt ttgccgatga
     1441 ccacgtcgct gccgatgatg ctgtaagggc cgatttccac gccttctgcc agttgggcgt
     1501 cgggcgcgac gatggcggtg ggatgaattt tcatggttca ccttccctgt ttttatgaat
     1561 gacggcttcc agctcttcca cgcgccggag cagtttttcc attttggcgc ggatttccgg
     1621 aagtttggcc ttgctgactt cgaccttcag ccattcccgg aacggaagaa acggcgtgcc
     1681 tccgccgacc atgcccgcgg gaatgctctt gtgaatgccc gaggaagcgc cgatcatcac
     1741 ggagtctccg atggtgatat ggccgacgat gccgacctgg ccgccgatga tgacgctttt
     1801 gccgattttg gtcgagccgg agatgccggt ctgcgcggcg atggccgaat tttcgccgat
     1861 gacgacgttg tgcgcgatct gcacgaggtt gtcgatcttc acgttgcgcc caatccaggt
     1921 tttgcccagc gtggcgcggt ccacggtggt gttggcgccc agctccacgt cgtcgtcgat
     1981 ttgaacgacg ccgacctggg ggattttttt gttctgcgac cccggcgcgg aaaacccgaa
     2041 gccgtcgccg ccgatgacca ccccggaatg caggatgacc cgccggccga tgaccgagcg
     2101 ggcgtaaacg gagacgttgg cgtacaggac ggaatcttct ccgaccgaag cgtcgcggcc
     2161 gataaatacg ccgggataaa gaaccgcgcc tttatccacg cgggcgccgc ggctgatgaa
     2221 cgcccggggc atgacggtcg cctccgggga gaccgaagcc ccttcttcga tatatgcgtc
     2281 cgggctgatg cccgcgggac cgtgatcgac cggataaaaa accgccagca gcttgccgaa
     2341 cgccgcgtag ggatcggcca cggcaatcag atttttatcc ggcgcgacgg ttcccggagg
     2401 gacgaggatc gcccccgcga gcgtggtttt gagccacttg aaatacttcg gattggagac
     2461 aaacgtcaga tcgtccgcgc ccgcctcctc gatcggacgg atatcgccga tcagcttctg
     2521 gccgtctccc gcaaccgcgc cgcccaccag ggcggctatt tccgcaatgc tttttttcat
     2581 ggtcttcagc gaggcttact tttttgcgcc cgacgttttg ttgaaatcat cgatgacctt
     2641 tttgctgatg tcgccggcct tgtcgaagta gggcagcggc atggtgctga tgtccatgat
     2701 cacggtgtat ttttcctttt cggcaatggc ccggacgact ttcaggattt cggggatcag
     2761 ttgctgcgaa tattcctgat cgcgtttctg cagctccttg ttggtgtcgt ccacgagaat
     2821 ctggtagtcg cgcatttttc gctggtaggc ggtttccttg tcgccgcgcg cgctggccgt
     2881 catcacggcg ccctgtttgt ccagttcgtc cttgagtttt ttcacctcgt tttccatgga
     2941 tttgatgttt tcctgtttcc gggcaaacag ctttttaaag tcttcggccg ctttcttgcc
     3001 cgcattggaa ttttgaatga tcgtctgcat attgatgaag ccgatcttgt cggcggccag
     3061 cgccgtgttc gtccaggcaa aaagaagcag cgtcagtccc gcgatcaaaa gcatattctt
     3121 tttcatctta aaatttcctc cttcagtcgg ttaaattact gttcagtaat gaaaatttct
     3181 ttcattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcca tcacataaac
     3301 atgccgatgg tgaattccca gcggcccttg ctgtcgtcgc tcagtccctt gttgtccagc
     3361 acgtagccgt actccaggcg aagcgggccg atgggagaat accagcggat gccggcgccg
     3421 caggtgcttc tcaggtcgtc gagataatag ccgccgttcc aggtgttgcc cgtgtcgtaa
     3481 aatacgacgc cctttattcc tgcgtctttg acgagcggaa aaaccacctc cgcggtcccg
     3541 gtgagcatgg tggtgccgcc gaccacgtcg ctggttcccg gattggtcgg ccccacgtag
     3601 cgcagaccgc gcagggagtt gatgccgccc agcacgtacc gttcatagat cggaatcttg
     3661 tcgtcgctgt cgtcattgtt ctgcagatac ccgatctggc ctttcaggga gaagaccatg
     3721 tcccagaaga gcggaacaaa gccgatcgtg ctggcgttgt atttgacgaa cttggtgttc
     3781 ccgcccagcg gcatgccggc atactggacg gatgcggtgg acttgaggcc tttgctgggg
     3841 aacatgacgt cgttggtggt gtcgtaggac atggtgaagg tgacggcgct cgtctttcgc
     3901 tcgccctcct gcaccttgat gtaatacgac gcggtggcat aattgatgtc ctggatgttg
     3961 tcgatgctgt acttgtagcc gacgtagccg acgattttcc agaacagcgg gtgccccagg
     4021 gtgacccccg cgcccatggt gtccagcgtg taggagtcgt attccttttt gtatttccag
     4081 acgtcggcct tgaaccacaa cggcagatcg aacaggaagg gttcggtgaa ggacagttcg
     4141 tagttgttgg ttttggagcc gagcgaggcc ttcaggctca tggtctgccc gtagcccagg
     4201 aagttctgct gaacgatctg cgccatgatc accgcctggt ccacggcgct gtaccccgcg
     4261 ccgatcatga acatgccggt gtttttttcc ttgacccgga tgttgatgtc catttggttg
     4321 tccggcgcct tttccgtctg gatgtccact tcttcgaaat agcgaagctg gttgaggttt
     4381 tcgtagcttt ttttcaactt ggtggaggaa tacaaatccc cctccacgat gaacagcgaa
     4441 cggcggatca ctttgtcgcg ggtggtggtg ttgccggaaa tgccgatgcg ggcgatgtgg
     4501 accagttcgc ctttcttcag gaggaagttc aaatccacca gctgctcctt ttcatgggtg
     4561 gtgacctggg gattgacgtc ggcattggcg tacccttcgt cgttggccgt tgtcgtgatc
     4621 atctccatgt ccttcatgat cgcttcccgg ctgtagtggc tgccgctttt ggtctttaac
     4681 tgcgcgtaca ggtcggcccg ggttttctcc agataatctc cggagatttc cactttgccg
     4741 attttgaagc gcttgccttc gcggacgaca atcttgatgt agatgccctt gtcgtcatgc
     4801 gtgatgaccg gctcgccgat ctgggcgttc acgaagccgt tgttgaaata aaaagcggtc
     4861 agccgctgaa catcctgctt gagctgctgg gattgcagga tgcccgtgtc cgtaatgaag
     4921 cccagcaggg ttcgctcgtt cgtggccatc atgccgacca gttctttggt cgtgaaggat
     4981 tcatttcctt caaacgtaat ggagcggata taaacccggt cgttttcctt gatgtccaga
     5041 atcaggacga aatccttctc gccgtctttt tccagacggt cggtaatttc ggcgttgtag
     5101 tagcctttgg tgtcgtaaag cgccttgatt ttttgcaggt cctgcttgat gttttccgga
     5161 ttgacgcttt gccgcacctt gacgctcatg gcttccagaa tgtcgtcctt gtccaccttc
     5221 ttgttgccga tcaggcggat ttccgaaatg aggccttttt cgaccacgtt gaaggtcagc
     5281 accttgcctg cggcctccgt cgcgacgtcg gcgctgacgt ccagaaaata ccccatcttg
     5341 aagatggttt tgatgtcgtc gctgatgtct tcctcatcca gcggatttcc gggcctgctt
     5401 ttgattttct gccggatggc gtccgcgccg atcttgcggt tgcccgcgat ttccactcgc
     5461 gcgattttat ccaggaggcc cgcccggtcc agaatttccc gcttcagcgc cgccgccagc
     5521 gctcgggcgc ccgcgtcgcc ctttccctga accgaaacgg ccggcaggat tttcccttcg
     5581 gcaacgtcga tgatttgcgc gtcgatgttc agggcttcgc cgaactgcat aatgctgccg
     5641 gtgacgacgt aatcggcgcc cagcgctttt cccgccgcgt ccgcttcctg tcggttgggc
     5701 acggggctgc ccggttcgaa gccccccgcg gtttttactt ccactttctt ttcccgcccg
     5761 aattccgtaa gcaggctgtt gtaaaccgac gcctgcagtt cggcgcttcc ggaaagcgcg
     5821 taaacctcaa aggggagaac gcatattttt ttggggccgt ccgcccgcgc ctccgccgca
     5881 aacaaaagaa gaagaaccgc gatgaagagc cggatcgttt tgaagttaaa aatcataaat
     5941 tttcccgtcg cgcagaccga ttctgccgga catcctttcc gccagcagtc tgttgtgcgt
     6001 gactacaacc agcgttattt ttttttcggc gttcaaggca atcagaaggt cttctatctt
     6061 ttttcctgtt tccgtgtcaa ggtttcccgt cggttcatcc gccagcagaa tttccggttc
     6121 catcgccagg gcgcgggcga tggccacgcg ctgctgctct ccgccggaga gctcccccgg
     6181 tttgtgccgg aggcgatgct ccaggccgac ctccgtcaaa agatgcgctg cccggtccag
     6241 ggcttttttt cgctcaagcc ctccgatgaa cgccggcatc atggcgtttt ccagcgccgt
     6301 aaactccggc agaaggttgt tgaactgaaa cacgaagccg atggttcggt tgcggaacgc
     6361 cgcgattttc ttttcattcc agttgaaaac cgtctggccg gcaatggaaa gcgagcctcc
     6421 ggtcggatgg tccagcgtgc cgagaatatg aatcagcgtg ctttttcccg cgccggacac
     6481 gccgacgacg gccagggagt cgcctctggc gatttccagg ttcaatccct tcaggacttc
     6541 aattttattg ccgtctttga cgaaggtttt ggataaattt tccagtttaa tcatatacgc
     6601 cgcaagaccc tgccgttatt cgtatctcag cgcttccgcc ggatccagcc gggaagccct
     6661 ccaggatgga tagatcgtgg acagaaaaca gatcacgagc gtgcccgcga caatcacggc
     6721 cacgtcgccg taattcactt ttgacggaag ctcgcttaaa tagtacacgt cgcccggcag
     6781 aattttgaaa ttaaaaactc tttccacaaa cagggaaatt ttctgaaggt tgagcgcgac
     6841 cgcaagcccc gcaatacagc cgaaaaaggt tcccaccacc ccgacgatca gcccctggta
     6901 gatgaatatt ttcatgatgt tggcggaggt ggcccccatg gatttcaaaa tggcgatgtc
     6961 cttgttcttt tccatgacga tcatgatgag cgcgctgatg atgttgaagg cggccaccag
     7021 cacaatcagg ctcagaatga tgaacatgac gcgcttctcc agcttgagcg ccgaaaacag
     7081 atttttgttc atctgcatcc agttctgggc gtaaaagggg aagcccagcc ggttctggat
     7141 ctggcgggcg atgacgtccg ccttgtagat gtcccgcacg acaatgtcga tgcccgaaac
     7201 ggcgtcgccc atttccagaa aatcctggca gctcgtcagc gaaaggaatg ccagtttgga
     7261 atcgtattcg taaaatcccg attcgtaaat gccgaccacg acgaattttt tcatgcgggg
     7321 gaccatgccc atcggcgtgg cgattccacc cgccggagaa atgatggtga tcgggtcgtg
     7381 cagaaaaatc cccaggtttc tggccatttc cctgccgatc agaatgccgg ccagctcccg
     7441 gtcggccgcg ggcagcttcc ggcggacggc ttcgggaatc cggtcgagat gcccgacgct
     7501 gccctggcgg attttgccca ggttgatgac ccggggcgcg ctttcggtgt ccatgccgcg
     7561 gatgatcacg ccggtgacgc cgttttggct tcggatcatg gcttccgtgt aaatgaaggg
     7621 cgtggaggcg acaacgcccg ggatccggtc gatttcccgg cggacgtccg ggtaattttt
     7681 catcgggctc atgtccgccg tcacttcaat gtgtgagcgg atgcccagaa ttttgctgcg
     7741 caaatcctcc tcgaagccgt tcatgacggc gagcacgatg atgagcgcgg ccacgcccag
     7801 aaaaatgccg aaaaccgata tgaaggtaat gatcgaaacg aagacctgtt tgcgtttcgc
     7861 tctcatgtac cgtctgctga taaaaaattc gtaaggcatc aggcattgtc ctgtcgggaa
     7921 gccgttaatc ttgacggctt cgtaaaaagt ctttttcgca c
//
LOCUS       DAOSRM010000093         7901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_8644_length_7901_cov_107.379684, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000093 DAOSRM010000000
VERSION     DAOSRM010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8644_length_7901_cov_107.379684"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..880
                     /locus_tag="PLA58_08640"
     CDS             236..880
                     /locus_tag="PLA58_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR62898.1"
                     /translation="MQTADLKMSEKEKKILDAAIGVISEKGFSAASTSEIARQAGVAE
                     GTIFRYFKTKKDILRGILMHLGNVIAELLVVYGLKKILFHEGHEDLRSGIKGVLKDRM
                     AMIDAVFPMLRIVITEAMYHEDIRQILYDKIISRAVEVFAEYHREMVDKGLMKGEIDA
                     VVAGRSILANLVLFFVYRNLFGPMIELDDYDRELDQVIDAIMHGIAMPENCDKK"
     gene            908..1951
                     /locus_tag="PLA58_08645"
     CDS             908..1951
                     /locus_tag="PLA58_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOR62899.1"
                     /translation="MKIKMSFFFLLCPVLFLMFGCGDKDAAPPEAAPVFVIAQKIEKK
                     TVARVIAVSGNIEGSKTARLGFMVSGKINYIAAREGAMIEKGQLLASLDPENYRIAKE
                     IADANAAQIQDEYDRLNLMHERGSLSESDYSKISNGLKMARAQQKLHTKNLAETRLYS
                     PFRGVLLKRGVEVGEIIGAGLPLFGVSDIRLVHVNASVPETELRFIKMDVKAKIYVAS
                     IDSAFSGRVIEVGSLAEPTTRAFSVRMEVPNPDGILRPGMTAEIQIPSGREEEVIRVP
                     GEAVFRDMDNTAYVFVVDDVKRRAFRRKISLGKIDENGIVVTAGLAPGELVVVGGQHK
                     LHNGSLIALKQAL"
     gene            1948..6459
                     /locus_tag="PLA58_08650"
     CDS             1948..6459
                     /locus_tag="PLA58_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOR62900.1"
                     /translation="MNFVKKSLKYPQVTLSILALTFLVGVYSLMEMPRREDAKVKVRV
                     GQVIALYPGANALQVEEQVTRKLEQYLFQYAEVRKEKTTSVTSDGMVVITVWLNNDVE
                     NFDPFWSKLNHQLNVLKAVSLPAGVYGPIVNSEFGDTEALIIAVEMDEPRYAELRDYA
                     QKLEDTLRTMKEVSKIRRLGEQKEQIVVFTDSAKMALYGVRLDQVMKILQSQNTIGPA
                     GDLSTSDARVPLYTQGYYKTEEEIGHQIVGFAKTGEVVRLRDVARIERRFEEASSKII
                     VNGRSSILLAVQAHEGYNIVAFGKAVDRKLHEASKWLPAGVKLNTIVNQPRIVERNVS
                     HFLFEFVLAIAAVILVIILLLPFRVAAVAATAIPMTVAMTFALLHFFGIELHQVSLAA
                     LIVVLGMVVDDAVVVADNYVELLDNGVRPQTAAWKSATELVVPILTATITIIAAFLPM
                     VMLSGMVKDFIIALPLTVSIALSASFVVAMVLTPVLCLTFIRKGLRNPAGEASPRPPR
                     RSVLNLMQSGYDRAIVWSMAHARLTILVCLGAMVMAVVLYQIIPQKFFPEAERNQLIV
                     DLWMPTGTKLKKTEIAIGGIQELIQRDPAVVSCATFVGSSIPRFYYNFMQGNPAANFA
                     QILVNTRRDDDAKRLRRELNRRADEVVPEGRVQARLLQQGVPSDASVEIRIIGEDLAT
                     MKSIGNKVEDILRRTQAVAFLRLDSREDYYGVAVRLKDGAERLGFTTESVAKSIYAGF
                     TGAPVSTLYEGNVAVDIVLRLDEKSRRHFGDVENLYLTSPVTGAGVPLRQIASLEPQW
                     QTGRINHLNGLRTLTVQCEPADGYLASQVLKMAGPRIAGLSLPTGYKIEYGGEYENQK
                     ETFSEMLTALVVSLFMIFLVLLFQFRNLKETFMVMLTIPLSMFGAMLGLLMTGNPFGF
                     TAFVGLISLSGIVVRNAIILLDHADELHRGGASIATAAIESGKRRLRPIFLTASAAAI
                     GVIPMILSGSPLWSPLASVIAVGIMLSMVISLFLVPVLYACLMKPAGEDRESPVSADP
                     AKNAVPGGKLLVLVMAAVCLLSASAGAQDLPEALNLQKVIDLAVQNNRLLAVKKLQVQ
                     EKQQKVNEDRVKFLPSVMVGGAYQYHGNIGELSLKQGTLGQATIGAISIPLPSADTTL
                     DLGHADTYTAGVLMYQPISQIPKIMAGVNVSKTDLEIAKNDQAKATLQVKQIAEKLYF
                     GLLIQEKRKEEARIKRDLARKKLYDVESAVLAGKTIPSSLTGLKANAADEEQNLLKIN
                     VQMEDYFDDLRRLTGLKSASFSLAPVLEDDADLNTAPAGVPGGQENQDLKIAALYQTK
                     AQHAVDAGRYSYLPDFGILGGYVYQDGAAGFTGDIRYPFLGSSLGLDPQWRKRDAFIG
                     VVLNWNLKDLVANTYITRQRLFLKKQAEENLANTREQIHADTEKARRKLTQAAELIGV
                     AGKVVEYRREDFKIQTDRFSAGLNLEADWLTAKAALAKAEADWLAARLNYRMAWTELQ
                     ILAGRYGGG"
     gene            complement(6444..7148)
                     /locus_tag="PLA58_08655"
     CDS             complement(6444..7148)
                     /locus_tag="PLA58_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOR62901.1"
                     /translation="MSQEIEANIQKIRERIAAAAARANRDPAGIRLMAVSKTVEPRRI
                     LKALEAGILLLGENYVQEAREKIPAVGRGAEWHMIGHLQTNKVKYVVHLFDWIHSVDR
                     LDLARELDKRAGQHNRRLNVLIEVNVSGEASKNGIEPSGVTDLVREISTLPNLVVRGL
                     MTMPPYSDNPEISRPHFQALRRLRDEINAAAIPNVRMDELSMGMTDDFEVAIEEGATI
                     IRVGRAIFGERRYPPP"
     gene            complement(7145..>7901)
                     /locus_tag="PLA58_08660"
     CDS             complement(7145..>7901)
                     /locus_tag="PLA58_08660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOR62902.1"
                     /translation="RERFETVPYIQPSTSRHPLFHCLKKCNGNFELTGHDYSGIKQPD
                     FEKKESIMVLSLSSPLILASASPRRKELLSAVGLTFKVRPADVDETGLPDENPRAHVR
                     RLSAEKAAVIARRYPKALVLGADTIVVIDGQILGKPRDQKQARAMMRQLSNRKHTVFT
                     GFTIACKQTGAAKTGVVQSAVYFKEISPDEMDWYVNSREPYDKAGGYAAQGMGASFIQ
                     AIRGSYTNVIGLPLCEVMEALKSMDAIHFREPA"
BASE COUNT         1717 a         2162 c         2309 g         1713 t
ORIGIN      
        1 tttttcccct ttgtaacgag gagttccgta tagccactcc gaaaattgat taatctatat
       61 ctcattgcgg ccaaatatcc aaaaggtcat ttttcttcat ggcgacggcg tcgcttaaaa
      121 aaggtattga caaaaaccaa aaaatgagtg attactcact caacaaggcg aaacggagga
      181 atatttttat aaatacatga aatcactttg ctttacaaaa tgaaaggggt tgatcatgca
      241 aactgctgat ttaaaaatgt cggaaaaaga aaaaaagatc ctcgatgcgg ccattggcgt
      301 catctcggaa aaaggcttca gtgcggccag tacgagcgaa atcgcgcgcc aggccggtgt
      361 cgcggaagga accattttcc gctactttaa aacaaagaaa gacattctgc gcggcattct
      421 gatgcatctg ggaaatgtca ttgcggagct tctggtcgtg tacggactca aaaaaattct
      481 tttccatgaa ggtcacgaag atcttcgctc cggcatcaag ggggtattga aagatcgaat
      541 ggccatgatc gatgccgttt ttccgatgct gcgcattgtc attacggaag ccatgtatca
      601 tgaggacatc cggcagatcc tctacgacaa aatcatttcc cgggcggtcg aggtgtttgc
      661 ggaatatcac agggaaatgg tcgacaaagg gctgatgaaa ggcgagatcg acgcggtcgt
      721 cgccggccgc agtattctgg ccaacctggt gctttttttc gtttaccgca acctgttcgg
      781 cccgatgatc gagctcgacg attacgaccg ggagctcgac caggtcattg acgcaatcat
      841 gcatggaatc gcaatgcccg agaactgtga caaaaagtag ggaaggcgtt tttttgtgaa
      901 ggtttttatg aaaattaaaa tgtcgttttt tttcttatta tgccccgtgc tttttctgat
      961 gttcggttgc ggcgataaag acgcagcccc gccggaggcc gcgcccgttt tcgtaattgc
     1021 ccaaaagatc gaaaagaaga cggtggccag ggtcattgcc gtcagcggaa acatagaagg
     1081 ttcgaaaacc gcccggctgg gcttcatggt gtccgggaag atcaactata tcgcggccag
     1141 agaaggggcc atgattgaaa aggggcaact gctggcgagc ctggatcctg aaaattaccg
     1201 gatcgccaag gaaatcgccg atgccaacgc ggcccagatc caggatgaat acgaccggct
     1261 gaaccttatg cacgaacgcg gaagcctgtc cgaaagcgac tattccaaaa tctccaacgg
     1321 gctgaaaatg gccagagccc agcagaagct gcatacgaaa aatctggccg agaccagatt
     1381 gtattctccc tttcgggggg tgctgctcaa gcgcggcgtg gaggtggggg aaatcatcgg
     1441 cgccggtttg ccgctcttcg gcgtttcgga tattcgcctg gttcatgtga acgcttccgt
     1501 gccggaaacc gagctgcgct tcattaaaat ggatgtgaaa gcgaaaattt atgttgcgtc
     1561 cattgattcg gcattttcgg gcagagtgat tgaagtcggg tccctcgcgg aaccgaccac
     1621 gcgggctttt tccgtcagga tggaagtgcc caatcccgac gggatcctgc ggccgggcat
     1681 gacggccgag attcaaatcc cgtccggtcg cgaggaggaa gtgatccgtg ttcccggaga
     1741 ggcggttttt cgtgacatgg acaacacggc ctatgtcttc gttgtggacg acgtcaaacg
     1801 gcgggccttc cggaggaaaa tctcgctggg gaagatcgac gaaaacggca tcgtggtgac
     1861 ggcgggtctt gcgcccggcg aactggtcgt ggtcggcggc cagcataaac tgcacaacgg
     1921 atctttgatc gcgctcaagc aggcattatg aattttgtca agaaatcgct caaatatcca
     1981 caggtcaccc tgtccattct cgccctgacg tttctggtcg gtgtgtattc cctgatggaa
     2041 atgccgcgcc gcgaagacgc caaagtcaag gttcgcgtgg ggcaggtgat cgccctttat
     2101 cccggggcca acgccctgca ggtggaagag caggttaccc ggaaactgga acagtatcta
     2161 ttccagtacg cggaggtgcg caaggaaaaa acgacctccg tgacctcgga cggcatggtg
     2221 gtgatcaccg tctggctgaa caatgacgtc gaaaatttcg atcccttctg gagcaaactg
     2281 aatcatcaac tgaacgtcct gaaggccgtc agcctgcctg cgggcgtgta cggcccgatc
     2341 gtgaattcgg agttcggcga cacggaagct ctaattattg ccgtcgaaat ggacgagccg
     2401 cggtacgccg agcttcggga ctacgcgcaa aaactggaag acacgctccg aaccatgaaa
     2461 gaggtgtcga aaatcaggcg cctcggcgag caaaaggagc agattgtcgt cttcaccgac
     2521 agcgcgaaga tggcgctcta tggagtccgg ctggatcagg tgatgaaaat cctccagtcg
     2581 caaaacacga taggcccggc cggcgatctc tcgacatccg atgcccgtgt ccctctttat
     2641 acgcagggat actacaaaac ggaagaggag atcggccatc agattgtcgg tttcgcgaaa
     2701 accggcgaag tcgtccgcct ccgggatgtg gcccgcatcg agcggcggtt cgaggaggcg
     2761 tcctccaaaa tcatcgtcaa cggccggagc agcatcctgc ttgccgttca ggcgcatgaa
     2821 ggatacaaca ttgttgcctt tggaaaggcg gtggaccgga aactccatga agcgtccaaa
     2881 tggcttccgg ccggcgtcaa actgaacacg atcgtcaacc agcccagaat cgttgagcgg
     2941 aatgtcagcc attttttgtt tgagtttgta ctggccattg ccgcggtgat cctggtcatc
     3001 attctcctgc ttcctttccg tgtcgccgcc gtcgccgcca cggcgattcc catgacggtc
     3061 gccatgacct tcgcgctgct gcattttttc ggcattgaac tgcaccaggt ctcgcttgca
     3121 gccctgatcg tagtactggg catggtggtg gacgacgcgg tcgtggtcgc cgacaattat
     3181 gtcgagctcc tggacaacgg cgtgcggccg caaaccgccg cctggaaaag cgcgaccgag
     3241 ctggtggtgc ccattctgac ggccaccatc accatcatcg cggccttttt gccgatggtc
     3301 atgctgtcgg gcatggtgaa agatttcatt atcgctttgc cgctgaccgt ctccatcgcg
     3361 ctttcggctt cctttgtcgt cgccatggtg ctgaccccgg tactgtgcct gaccttcatt
     3421 cgcaaaggcc tgcgcaatcc cgccggggaa gcctccccgc ggccgccccg cagatccgtt
     3481 ctgaacctca tgcaatccgg ctatgaccgc gccatcgtct ggagcatggc gcatgcccga
     3541 ctgaccattc tggtctgcct cggggccatg gtcatggcgg ttgtcctcta ccagatcatc
     3601 ccgcagaaat tttttccgga agctgaacgc aaccagctga tcgtggattt atggatgccc
     3661 accggcacca agcttaagaa aacggagatc gcgatcggcg gaattcagga attgattcag
     3721 cgcgacccgg ccgttgtgtc ctgtgcgacg tttgtcggtt ccagtattcc gcgcttttac
     3781 tacaatttca tgcaggggaa tccagcggcc aatttcgcgc agatcctcgt caatacgcgc
     3841 cgggatgacg acgccaaacg gctgcgtcgc gagttgaacc ggcgggccga cgaagtggtc
     3901 ccggaaggaa gggtccaggc ccggctgctg caacaggggg ttccctcgga cgcctccgtc
     3961 gaaatccgga ttatcggcga agacctcgcg acgatgaagt ccatagggaa caaagttgag
     4021 gacattcttc ggcggacaca agccgtcgcc tttctccgtt tggacagccg ggaggattat
     4081 tacggcgttg ccgtccggct caaagacggc gccgagcgcc tggggttcac caccgaaagc
     4141 gtggcgaaat ccatctatgc gggattcacc ggcgctcccg tatccacgct gtacgaaggg
     4201 aatgtggctg tggacatcgt gcttcgcctg gatgaaaaaa gtcggcggca tttcggcgac
     4261 gtggaaaacc tgtatctgac gtctcccgtg accggcgcgg gcgtgccgct tcgccagatt
     4321 gcctccctgg aaccacagtg gcagaccggc cggatcaacc atctcaacgg gctgcgtacg
     4381 ctgaccgtgc agtgcgagcc ggcggacggc tatctggcct cgcaggtgtt gaaaatggcc
     4441 ggtccgcgga tcgcgggcct ttccctgcct acgggttaca aaatcgaata cgggggagaa
     4501 tacgaaaacc agaaagaaac cttttccgaa atgctgacgg ctctggtcgt cagtctgttt
     4561 atgatttttc tggtgctgct tttccagttc cgcaacctga aggaaacgtt tatggtcatg
     4621 ctgactattc cgctcagcat gttcggggcc atgctgggcc tgttgatgac cggcaacccg
     4681 tttggattta ccgcgtttgt cggcctgatc agcctctccg gcattgtggt cagaaacgcc
     4741 atcatcctgc tggaccatgc cgacgaactg caccgcggcg gggcgtccat cgcaacagcc
     4801 gcgatcgagt ccggaaaaag gcgattgcgc cccatctttt tgaccgccag cgccgcggcg
     4861 atcggtgtga ttcccatgat cctttccggc tctccgctgt ggagcccgct ggccagcgtg
     4921 attgcggtag gaatcatgct ttccatggtt atttccctgt tcctggttcc ggtgctgtac
     4981 gcctgcctga tgaagcctgc cggtgaagac cgggaatcgc cggtcagcgc tgatccggcg
     5041 aaaaatgcgg tcccgggggg aaagttgttg gtccttgtca tggccgccgt gtgtctgctg
     5101 tccgcctctg ccggcgcgca ggaccttccc gaagctttga acctgcaaaa ggtgattgac
     5161 ctggctgtgc aaaacaacag gttgcttgcc gtcaagaaac ttcaggtgca ggaaaagcag
     5221 cagaaggtaa acgaggaccg ggtgaaattc ttgccgtcgg tgatggtggg cggcgcctat
     5281 caataccacg gcaatatcgg tgagctgtca ctgaagcagg ggactctcgg ccaggcgacc
     5341 atcggagcga tcagtattcc gcttccgtcg gccgacacaa cgctggatct gggacacgcg
     5401 gacacctaca cggcgggagt gctgatgtat cagcccattt cgcagattcc gaagatcatg
     5461 gccggggtca acgtgtccaa aacagatctg gaaatcgcca aaaacgatca ggccaaagcg
     5521 accctgcagg tgaagcagat cgccgagaaa ctctatttcg gcctgctgat ccaggagaag
     5581 cggaaagaag aggcccggat caaacgggat ctggcccgaa agaagcttta cgacgtggaa
     5641 agcgccgtgc tggccggcaa aacaatcccg tccagcctga ccggactgaa ggcgaatgcc
     5701 gccgatgaag agcagaatct tctcaagatc aatgttcaga tggaggatta ctttgacgat
     5761 ttaaggcggc ttaccggttt gaaatcggca tccttttcac ttgcgcccgt gctcgaggac
     5821 gacgcggacc tcaacaccgc tccggccggt gttcccgggg gacaggagaa tcaggatttg
     5881 aaaattgccg ccctgtatca aacgaaagcc cagcacgcgg tggatgccgg acggtacagc
     5941 tacctgccgg atttcggcat tttgggaggg tatgtgtatc aggacggcgc cgctggcttt
     6001 accggcgata tccggtatcc gtttctcggc tccagcctcg gactggatcc ccagtggcgc
     6061 aagcgggacg cttttatcgg cgtggttttg aactggaatc tgaaggatct ggtcgccaac
     6121 acctacatta cacggcagcg cctctttctg aaaaagcagg ccgaggagaa cctggccaat
     6181 acccgggaac agatccatgc cgatacggaa aaagcgcgcc gcaaattgac gcaggccgcc
     6241 gaactcatcg gcgttgccgg aaaagtcgtt gaataccgcc gcgaagattt caaaattcaa
     6301 accgaccggt tttccgccgg tctgaatctg gaagcggact ggcttacggc aaaagccgct
     6361 ctggcgaagg cggaagcgga ttggttggcc gcgcgattga actaccgcat ggcctggacg
     6421 gagcttcaaa tccttgccgg ccgctatgga gggggatagc gtcgttcccc gaagatggcg
     6481 cggccgacgc ggatgatggt tgccccttct tcgatggcca cttcaaaatc gtccgtcatc
     6541 cccatggaaa gttcgtccat ccggacgttc gggatggccg cggcgttgat ttcatcccgc
     6601 agtctgcgca gggcctggaa gtgcggacgg gaaatttccg ggttgtcgga atagggcggc
     6661 atcgtcatca acccccggac gaccaggttg ggaagcgtag aaatctcccg gaccagatcc
     6721 gtgacgccgg acggttcaat gccgtttttc gaggcttcgc cgctgacatt gacttcgatg
     6781 agcacattca agcggcggtt gtgctggccc gcccttttgt ccagctcccg cgccagatcc
     6841 aggcggtcca cggaatgaat ccagtcgaag agatggacga catatttaac cttgttggtc
     6901 tgcaggtggc cgatcatgtg ccattcggcg ccgcggccga cggcggggat tttctcgcgg
     6961 gcttcctgga cgtaattttc gcccagcagc aaaattcccg cctccagcgc cttcaggatg
     7021 cgccggggct ccacggtttt gctcaccgcc atcagccgga tgcctgccgg gtcgcgattc
     7081 gcgcgggccg cggccgcggc gattctttcg cggatcttct gaatgttggc ttcgatttcc
     7141 tggctcatgc cggttccctg aaatgaatag cgtccatgga tttcagcgct tccatgacct
     7201 cgcaaagcgg aagtccgatg acattggtgt aggagcctcg aatggcctga atgaaagaag
     7261 cccccatgcc ctgggcggca tagcctccgg ctttgtcgta cggctcccgg ctgttgacgt
     7321 accagtccat ttcgtcggga ctgatctcct tgaaatacac ggcggactgc acgacgcccg
     7381 ttttggcggc tcccgtttgc ttgcaggcaa tcgtaaagcc cgtaaagacc gtgtgtttgc
     7441 ggttgctcaa ttgccgcatc atcgcgcgag cctgcttttg atcgcggggc ttgcccagaa
     7501 tttggccgtc gatcacgaca atggtgtccg cgcccaggac cagggccttc gggtatcggc
     7561 gggcgatgac ggccgccttt tcggcggaca gccgccggac gtgcgcacgc gggttttcat
     7621 ccggaaggcc ggtttcgtcc acgtcggccg gccggacttt aaacgtcaaa ccgacggcgc
     7681 tgagcagttc tttccgacgc ggcgaggcgg aggccagaat caggggagat gacagggaca
     7741 gaaccataat gctttccttc ttttcaaagt cgggttgttt tattccagaa taatcatggc
     7801 ctgtcaattc gaaattgccg ttgcattttt tgaggcagtg gaatagggga tggcgactgg
     7861 tggatggttg aatgtaggga acggtttcaa accgttccct a
//
LOCUS       DAOSRM010000094         7633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_9014_length_7633_cov_97.224861, whole genome shotgun sequence.
ACCESSION   DAOSRM010000094 DAOSRM010000000
VERSION     DAOSRM010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9014_length_7633_cov_97.224861"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /gene="rnpB"
                     /locus_tag="PLA58_08665"
     ncRNA           <1..221
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLA58_08665"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(270..479)
                     /locus_tag="PLA58_08670"
     CDS             complement(270..479)
                     /locus_tag="PLA58_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62903.1"
                     /translation="MDTFLKLFPFLHLVVLAAGAILLLVVKRKYPKVRNSELITVFIL
                     FLILVAVFTEQGLDWIKRLMSLLQA"
     gene            complement(595..1908)
                     /locus_tag="PLA58_08675"
     CDS             complement(595..1908)
                     /locus_tag="PLA58_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOR62904.1"
                     /translation="MSQTKDKKSLPVAGVFFLLIVIPLSLMAFLIANGMFKLGVTIKE
                     RAVNVLDVKSQEDIKARAINTANQVANLLMESKKDLQVATIIPSTEAVYKQFVAENKA
                     PLWVKKDGKVQQVLTPLYKEIALIDKNGYERIKIVGGQAAPAEKLVDVSKPANTTYKN
                     EDYFAKTKNLNRGEVYVSPVTGWYVNKAAFDKGERFNGVVRFATPVFNQDGFSGMIVL
                     SLDYRHLGQITDQLIPTQAERVFETDASSGNYAYMFDYRGNVIAHPNDFHIVGLNPDG
                     TPVPTITQQNVKELTDKGADALNIIQAGFLDPNLTALAKEAAEGKTGFKTYKLFGMTK
                     FVAYAPIKFYASNIPEPAGFGGVCLGVEVEKYNEAALKVSQNIEKESKAWTATVILVL
                     IVAMVILFFIMLILVRGISRSIQSEVPEGSEGDLAGLNDDDDDDK"
     gene            complement(1960..2337)
                     /gene="atpE"
                     /locus_tag="PLA58_08680"
     CDS             complement(1960..2337)
                     /gene="atpE"
                     /locus_tag="PLA58_08680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOR62905.1"
                     /translation="MSHKHGKTILTVFTSVAAMLLSSQFAFAADAIPVGGESYIKVIF
                     AVGAMIGAGLAIGLGAIGAGAGIGNAANGACQAVGRNPGVQGKIMMVMLVGMAMAESI
                     AIYALVIALILLYANPFKPFFLG"
     gene            2845..3018
                     /locus_tag="PLA58_08685"
     CDS             2845..3018
                     /locus_tag="PLA58_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62906.1"
                     /translation="MQELLTVVTYPVVWLICSYLLKMESGLAKLLISLTAAVLVYVLY
                     GIWQSQKEKHKEN"
     gene            3085..5589
                     /locus_tag="PLA58_08690"
     CDS             3085..5589
                     /locus_tag="PLA58_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR62907.1"
                     /translation="MTSLVVILLILLLALTVNNAREKEMADLFSRQQLASAQNASARM
                     TEILSQVERNVALFSYFDPNGKILSPQDHRKIEILYSVWGKTIDAVLVFDAQERVRRV
                     LSQDALPRVNLIPHFQALKSQKRQYLALSGAGSGGAPTQKQDWYLIWGYPIWREGHIF
                     DGAWMVSFSLAALVHAGERQIQENRLGDLWLINDRGQIIMHPHLPTIGKNTGDLIHDR
                     RGNAIAFSPEGGRHIDAKILQRSGSPQRSVIAYYPFQVAGNHWFVMVSAPYAQVVAPV
                     RTTFLYTMFSAFVLMLILVIVGIFYAFREGRRLRMREVQERLKERETWQEKLLREKKT
                     IEGIIEGSPIPSFVINKEHQVILWNRACVELTGQSAEDTLGTDGHYRAFYPVKRPMVA
                     DLIIDADIDALNKYYSAKQVKKSEKVLGAYEAYDYYENLGGRSRYMYFLAAPIYDEDG
                     NIIAAIETLQDISREQELTRSLQEYAETLQNELAENIELRKEIEGLLNYMQSIVRSLP
                     DKIYELDQNGIITFISWGLKKPKSELRGKHFLEFVAPENADFVVSRWEDAKQGIYTPY
                     EIETVAKDGHKVNLMITTSPVIGTDHYILVQRDITEFKNLEKKLYSSQKLAALGQLSA
                     GIAHEIRNPLSSIKMSLQILEKRIKPEGNDLKRFKIAQKEVEHLEELVNNVLVFAKPM
                     EPKIESVDLTKVLEQAVALSEQIIHDKKIEVTLETRDAPAVNVDVAMITDAFVNVIRN
                     AVEAMEEGGHIHIYARSIGSRPPSVLVVVEDDGCGIDDADMPHLYNPFFTKKKYGTGL
                     GLSQVLKIVEVHQGKLEIISEKGKGTKVCVTLPCNK"
     gene            5741..7192
                     /locus_tag="PLA58_08695"
     CDS             5741..7192
                     /locus_tag="PLA58_08695"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOR62908.1"
                     /translation="MARSLGVREKARAVAQTEKVKIMASILVIDDDASIAETLDLYLT
                     EEGYSVRTALTGTDGLNKYVQEPSDVVILDIRLPDVDGFAVLEDLKEENENVKVIMIT
                     AFHDMETTINAMKGGAFDYIHKPVNVDELDLAIKKALKSLEMEKKIDGLLMEPSRSFR
                     VGDIIGTGNEMREIFKTIGTVSQSRTTVLIHGESGTGKELIAKVIHNNTSPNEPFIAV
                     NCSAIVETLLESELFGHEKGSFTGAVARKLGKFELARFGSVFLDEISEMSVNLQAKLL
                     RVLQEMEFERVGGKDRVKVNARIMAATNKDLRAMVKEGKFRDDLFYRLNIVSIHLPPL
                     RSRRQDIPPLIDYLLSKINLDLHKKIIGVSGEMMEKFMTYSWPGNVRELENLLVRACV
                     IAKGQVLGVGDFPELGKDYEALPDKKDTTEEVFTPEEPGKFLTLDQVEERYIRKIIKE
                     TNKNKGEICETLGISRPTFERKLEKYGITFERD"
     gene            complement(7286..>7633)
                     /locus_tag="PLA58_08700"
     CDS             complement(7286..>7633)
                     /locus_tag="PLA58_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62909.1"
                     /translation="GIILIGEPYWRQLPPTEDVAKGCLAHSISDFLLLPELLASFGRL
                     GYDVVEMVLADQNGWDRYEAAKWLTMRRWLEANPGDELANEVRAQLTSEPERHAAYTR
                     EYMGWGVFALMTR"
BASE COUNT         1876 a         1930 c         2096 g         1731 t
ORIGIN      
        1 ggcgaggtaa gagctcaccg cttttccggt gacggaaaag gcatggtaaa ccccatccgg
       61 agcaagacca aataggagaa cgtttgaggg cggcccgcct gatgttctcg ggtagtgtcg
      121 ctagaggcgg caggcaactg ccgtcccaga tgaatgatca tcgccgcggc gagggtaacc
      181 gaatcgcgga acagaactcg gcttatgatt ttctccgcgc ctgcttgtta tgacgagccg
      241 cggcccctgg atgaaggggc atccgtttgt caggcctgaa gaaggctcat caaccgtttg
      301 atccagtcca gcccctgctc cgtaaagaca gccacgagga tcaggaagag aatgaagacc
      361 gtgatcagct cggaattgcg gactttgggg tattttcttt ttacaaccaa aaggagaatg
      421 gcgcccgccg ccagcaccac caggtgcaga aaggggaaaa gcttcagaaa agtatccatt
      481 cggatctcct tgggagcagg attgctcaac aaccttcaga aaaagccgtg agggcccaat
      541 caataaaaaa ggccgaatcg aaattcggcc tttttgttct tgagtgaaag ctacttattt
      601 gtcgtcatcg tcgtcgtcat tcagtcccgc cagatctcct tccgagcctt ccggaacttc
      661 cgactgaatg gagcgggaaa ttccgcgcac caggatcagc atgatgaaga acaggatcac
      721 catggccacg atgagcacca gaatgacggt ggccgtccac gccttggatt ccttctcgat
      781 gttctgagag accttcaggg ccgcttcgtt gtacttctcg acttccacac ccagacagac
      841 ccctccgaaa ccggcgggct caggaatgtt tgacgcgtaa aatttgatcg gcgcataggc
      901 gacgaacttg gtcattccaa ataatttata cgtcttgaat cctgttttcc cctcggccgc
      961 ctctttcgcc agagccgtta aattgggatc caggaaaccg gcctggatga tgttcagggc
     1021 atcggcgccc ttgtccgtca gttctttcac gttttgctgc gtgatggttg gaaccggcgt
     1081 tccgtccgga ttcagcccca cgatatgaaa atcgttcggg tgagcaatca cgttgccgcg
     1141 gtaatcaaac atgtaggcgt aattaccgga tgaagcatcc gtttcaaaca cccgttccgc
     1201 ctgcgtgggg atcaactgat cggtgatctg gcccaggtgg cgatagtcca gggacagcac
     1261 gatcatcccg gaaaaaccat cctgattgaa caccggcgtt gcaaaacgga cgacgccgtt
     1321 gaaccgttct cccttgtcaa atgccgcttt gttcacgtac cagccggtga ccggagatac
     1381 atagacttcg ccccggttca gattttttgt ttttgcaaaa taatcttcgt ttttgtaagt
     1441 ggtattggcc ggtttgctta cgtcaaccag tttttccgcc ggcgccgcct gaccgcctac
     1501 gattttaatt ctttcgtagc cgtttttgtc aatcagggcg atttccttgt acagcggtgt
     1561 cagcacctgc tgaactttgc cgtctttctt gacccagagc ggcgctttgt tttcggcaac
     1621 aaactgcttg tagactgctt ctgtcgaggg aataatggtt gcaacctgca aatctttctt
     1681 gctctccatg aggagattgg caacctgatt tgccgtgttg atggctcttg ctttaatatc
     1741 ttcctgagac ttgacatcaa gaacgttaac ggccctttct tttatggtaa cccccagttt
     1801 aaacatgccg ttagcgatta agaacgccat cagtgataag gggattacaa tcagcaggaa
     1861 gaatacgcct gcaacaggta atgacttctt atctttagtt tgtgacatta tgatttcccc
     1921 ctaacctagg ttaattttgt agttaacttc cctgaaacat taacccaaaa agaaaggctt
     1981 gaaggggttg gcatacaata agatcaaggc aataaccagc gcgtaaatgg cgatcgattc
     2041 agccatggcc atgccgacga gcatcaccat catgattttg ccctgtacgc cggggtttct
     2101 tcccaccgcc tggcaggcgc cgtttgccgc attaccaatg cccgcgcccg cgccgatggc
     2161 gcccaggccg atggccagac ccgcgccgat catggcgccg accgcaaaaa tcaccttaat
     2221 gtaggattcg ccgccgaccg gaatcgcatc cgccgcaaag gcaaattggc tcgaaagcag
     2281 catcgccgct accgatgtga agaccgttaa aattgttttt ccatgtttgt gtgacattag
     2341 ttgactcctt tcatgacatg ttgtttattg tattcctttt ttgcgctcca tagcacatta
     2401 tccagggctc gcaaagaaga attaacatca atcggctcga cgctaatgca tgagatatgc
     2461 catggtttta acgaagaggg tttgtttttc ggcatcgaat cgacgccgcc tgcaaattcg
     2521 ggaagacggc tcaatttatt gaacttcctg ttccttcgct ttcggcgccg ggtgtcaaaa
     2581 aaagcgcagg gaaaatgctt tttcattttc tgtatgacgc cgcggaaggg aaaatgaaac
     2641 gccgcgttta atgttttgtt caatagcctg ccataaaaca ttaaatggtc gcaaacggag
     2701 agggaacgtc ctatgcgcag atccgcgtga aaaacctttt tgatatttca gttgaaattt
     2761 tgaagcaatg tcgctacgat gcccgcactt gtgccaaatc atgggacatc agagagtcat
     2821 tgtcatggat catagagggg cattatgcag gagctgttaa cggttgtcac gtatccggtt
     2881 gtctggctga tttgttctta tcttttaaaa atggaaagcg gtctggcaaa actgctgatt
     2941 tcactcacgg cggcggttct ggtgtatgtt ttatacggca tttggcagtc acaaaaagaa
     3001 aagcataagg aaaactaaaa ttcagtggcc ttgctgagaa actcataccg attcctgcgt
     3061 caagcgttca gtctgcgcat ctggttgacc agtctcgttg ttatcctcct gattcttctg
     3121 ttggctttga ccgtcaacaa cgcgcgggaa aaggagatgg cggatttgtt cagccgccag
     3181 cagttggcgt ccgctcaaaa cgcctccgcc cggatgacgg aaattttgtc gcaggtcgaa
     3241 agaaacgttg ccctgttttc ctattttgat cccaacggga aaatcctgtc gccccaggac
     3301 catcgaaaaa tcgaaatcct ttattccgtt tggggaaaga cgattgatgc cgtgctggtg
     3361 tttgacgccc aggagcgcgt ccggcgcgtc ctgtcgcagg atgcgctgcc ccgcgtcaac
     3421 ctgatccccc atttccaggc gttgaaaagt cagaaaaggc agtatttggc tttgtccggg
     3481 gcgggctccg gcggcgcccc gacccaaaag caggactggt atttgatatg ggggtatccc
     3541 atctggcgcg aggggcatat ttttgacggc gcatggatgg tgtccttttc tctggccgcg
     3601 ctggtccatg ccggagaaag gcaaattcag gaaaaccggc tgggcgatct gtggctgatc
     3661 aacgaccggg ggcagatcat catgcatcct catttgccca ccatcggcaa aaacaccggt
     3721 gatctcatcc acgaccgccg gggaaacgca attgccttct cgccggaagg cggcaggcat
     3781 atcgatgcga agattttgca gcgcagcggc tcgccgcaaa gaagcgtcat tgcctactat
     3841 ccgtttcagg ttgccggaaa ccactggttc gtgatggtga gcgctcctta cgcccaagtc
     3901 gtcgcgcccg tgcgcacgac atttctttac accatgttca gcgcgtttgt gctgatgttg
     3961 atcctggtga tcgtcggcat tttctatgcg ttccgcgagg gaaggcgatt gcggatgcgg
     4021 gaagtgcagg agcggctgaa ggagcgtgaa acctggcagg aaaagctgct gcgggaaaag
     4081 aaaacgatcg agggcattat tgaaggatcg cccattccca gttttgtgat caacaaagag
     4141 catcaggtga ttctgtggaa cagggcctgt gtggagctga cggggcaatc ggcggaagat
     4201 acactgggga cggacggtca ctaccgggcg ttttatcccg tcaaaaggcc catggttgcc
     4261 gacttgatca tcgacgccga cattgacgcg ctcaacaagt actacagcgc caagcaagtc
     4321 aagaaatcgg agaaggtgct tggtgcctat gaagcctatg attattatga aaacctcggc
     4381 ggccgcagcc ggtacatgta tttcctcgcc gcgccgattt atgacgagga cgggaacatc
     4441 atcgcggcga tcgaaaccct tcaggatatc tcgcgcgagc aggagctgac gcgaagcctg
     4501 caggaatatg ccgagacgct gcaaaacgag ctggcggaaa atatcgaatt gagaaaagag
     4561 atcgaaggtc tcttgaatta tatgcagtcc atcgtcagaa gcctgccgga taagatttac
     4621 gagctggacc aaaacggcat catcaccttc atcagctggg ggctgaaaaa gcccaaaagc
     4681 gagctgcgcg gaaagcattt cctggaattt gtcgcgccgg aaaacgccga ctttgtcgtt
     4741 tccaggtggg aggacgccaa acagggaatc tacacgccct acgaaattga aaccgtggcc
     4801 aaggacggac acaaggtcaa tttaatgatc acgacgtccc cggtcatcgg aaccgaccat
     4861 tacatcctgg tgcaaagaga catcaccgag tttaagaacc tggagaagaa actatacagc
     4921 agccagaagc ttgcggcgct gggtcagctg tccgccggga ttgcccatga aatccgcaat
     4981 ccgctttcat ccatcaagat gagcctgcag atcctggaaa agcgcatcaa gccggaaggc
     5041 aatgatctga agcggtttaa aatcgcgcaa aaggaagtcg agcacctgga agagctggtg
     5101 aacaatgttt tggtgttcgc caagccgatg gagcccaaaa tcgagtcggt tgatctgacg
     5161 aaagtcctgg aacaggcggt ggctctttcc gagcagatta ttcatgataa aaaaatcgaa
     5221 gttaccctgg aaacccggga cgctcccgcg gtgaacgtgg atgtggcgat gattacggac
     5281 gcttttgtca atgtgatccg caacgcggtg gaagcgatgg aggaaggcgg tcacattcac
     5341 atttacgccc gcagcatcgg gtcccggccg ccttccgtgc tggtggtcgt ggaggacgac
     5401 ggctgcggca tcgatgacgc ggacatgccc catctgtata acccgttttt tacaaagaag
     5461 aaatacggga caggcctggg cctgtcgcag gttttgaaga ttgtggaagt gcatcagggg
     5521 aagctggaga ttatcagcga gaaaggcaag ggcacgaaag tttgcgtcac attaccctgc
     5581 aataaatgac gggaggccgt tgcataatgt ctctgtgtca aaagcgaacg aagtgaagca
     5641 atctcatcag tatttgatcg tttgaagaga tcgccacgtc gcttcgctcc tcgcgatgac
     5701 aaaacggtaa tgatgcaaaa ttctcgacgg atcgttttga atggcgcgaa gcctgggcgt
     5761 tcgggagaaa gcgcgggccg tcgcccaaac agaaaaggtt aaaattatgg cgagcattct
     5821 ggtgattgat gacgatgcat ccattgcgga gacgctggat ttgtatctaa ccgaagaggg
     5881 atacagcgtt cggacggcgc tgaccggtac cgacggtctg aataaatacg tgcaggagcc
     5941 ttcggacgtg gtcatcctgg acatccgttt gccggatgtg gacgggtttg ccgttctgga
     6001 agatctcaag gaagaaaatg aaaacgtcaa agtgatcatg atcaccgctt tccacgacat
     6061 ggaaacgacc atcaacgcca tgaagggcgg cgcctttgac tacattcaca agccggtcaa
     6121 cgtggatgag ctggatctgg cgatcaagaa agccctgaag tccctggaga tggagaaaaa
     6181 aattgacggg ctgctgatgg agccgtcgcg cagtttccgg gtgggagaca tcatcggcac
     6241 cggcaacgag atgcgggaaa ttttcaaaac gatcggcacc gtatcccaaa gccgaacgac
     6301 ggtgctgatt catggagaaa gcggcaccgg caaggaattg atcgccaaag tgattcacaa
     6361 caacacctct ccgaacgaac cgtttattgc cgtgaactgc tccgccatcg tggagacgct
     6421 tttggaatcg gaattgttcg gccacgaaaa ggggtccttt accggcgccg tcgcccgaaa
     6481 gctgggcaaa ttcgagctgg cccggttcgg ttcggtgttc ctcgacgaaa tcagcgaaat
     6541 gtcggtgaac ctgcaggcga agcttttgcg cgttttgcag gagatggaat tcgagcgggt
     6601 cgggggcaag gacagggtga aggtcaatgc ccgcatcatg gctgccacga ataaagacct
     6661 gcgggcgatg gtcaaggagg ggaaattccg ggatgacctg ttttaccgtt tgaacattgt
     6721 ttccatccat ttgccgccgc tgcgcagccg gcgtcaggac atcccgcctt tgattgatta
     6781 cctgctttcc aaaatcaatc tggatctgca taaaaaaatc atcggcgttt ccggcgaaat
     6841 gatggagaag tttatgacgt attcctggcc gggcaatgtc cgtgagctgg agaacctgct
     6901 ggtccgggcg tgcgtcatcg ccaaaggcca ggtgctgggc gtcggggatt tccccgaact
     6961 gggtaaggat tatgaggcgc tcccggataa gaaagacaca acggaagaag tgttcacgcc
     7021 ggaagaaccg ggcaaattcc tgacgctgga tcaggtggaa gaacgctaca ttcgcaagat
     7081 catcaaggaa accaataaga ataagggtga aatctgcgag acgctcggca tttcccgccc
     7141 cacgttcgag cgcaagctgg aaaaatacgg catcacgttt gaacgggact aacccgcgcc
     7201 ccgtaattca agcatgtttc aagggcaacg atatagatca gcgggtgaac attcactacc
     7261 cggctacggc ccggtcatcg ccgtattacc gcgtcatcag cgcgaacacg ccccagccca
     7321 tgtattcacg cgtgtaagcg gcgtggcgct cgggttccga ggtcagttgg gctcgaacct
     7381 cgttggctaa ctcgtcgccg ggattggctt caagccatcg gcgcatggtg agccatttgg
     7441 cagcttcgta tctgtcccag ccgttctggt ccgccaaaac catttccacg acgtcgtagc
     7501 caagacggcc gaaagacgcg agaagttccg gaagcagaag aaagtcggag atggagtggg
     7561 caagacaccc tttggcaaca tcttccgtcg gcggcaactg ccgccagtag ggctcgccga
     7621 tgaggatgat ccc
//
LOCUS       DAOSRM010000095         7626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_9023_length_7626_cov_107.530841, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000095 DAOSRM010000000
VERSION     DAOSRM010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9023_length_7626_cov_107.530841"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1286
                     /locus_tag="PLA58_08705"
     CDS             93..1286
                     /locus_tag="PLA58_08705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOR62910.1"
                     /translation="MIKNQTPEIFLKSGRQAALLRGHPWVFSGAVAKIRGNPSDGEIV
                     LARDTGGQALALGFFNSRTDISFRVVSRDTGSPVDESFWNRRVLDALELRRQIIREGT
                     NCYRLINAEGDGFPGLIVDVYNDTLVLSVTTAGMERQKQTILDALLFHLKPARIYEQS
                     AGRSRGLEGLQERRGFLHGSDQEGAARVTENGHRFDVDFISGQKTGFFLDQRVNRETI
                     GALSRGRMVLNCFSYTGAFSVYAAAGKAKKVVSLDISKSACDAAAEHLRINGCKPEDH
                     PVIEADVFDYLRNLRECFDLIVLDPPAFAKIKRDVAKASRGYKDINLQAIRHLAPGGL
                     LATFSCSNFMEEDLFGKIVQGAARDAQADLQLLARLEAGPDHPLAAGHPEGRYLKGLL
                     LRKPA"
     gene            complement(1332..1727)
                     /locus_tag="PLA58_08710"
     CDS             complement(1332..1727)
                     /locus_tag="PLA58_08710"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HOR62911.1"
                     /translation="MKTKMHILIVSVFLLFGCDATMVMGGKVMGVSSGRFIYQDGYLS
                     TQYKADIDQVWQASEKTVTELKGSKIEKNRKIASGSIKAVIADEKVTILVEYVEKDVT
                     HVSVMCGVGGSNIASRMIQDKIAANIVMP"
     gene            complement(1785..2630)
                     /locus_tag="PLA58_08715"
     CDS             complement(1785..2630)
                     /locus_tag="PLA58_08715"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOR62912.1"
                     /translation="MSAQRNSILCPNCRKLISLDEPACPYCGLVRPGLHETAGKLQNI
                     FFAFEPVQTILYVNIAFFVLALLMDLRGIFTGGSPLAFLSPSNQSLLALGASGAIPVM
                     YYHNFWSVVSASFLHGGILHIVFNMMALYQLGPFVLREFGLHRFVSIYILTGIIGFAV
                     SVLAGVQFTIGASASICGLIGAIIYFGKSRGGSYGEAIFKQSLGWVVGLIIFGFLFSG
                     INNWAHGGGLLSGLLLSYAMGYNDQKSESAWSKILAYACVLITVAVLLWAAGSSVYYK
                     FLTRV"
     gene            2758..3915
                     /locus_tag="PLA58_08720"
     CDS             2758..3915
                     /locus_tag="PLA58_08720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOR62913.1"
                     /translation="MSIKESCRLNITSVAFGGAGVGRADGLVIFVPFTAAGDVADVEI
                     VERRKKFARGRLLGILEPSPQRTEPLCRYFGRCGGCSYQHIAYRHQLNIKQAQVKEAF
                     LKIGKVPEPPVEKVIASPCAYAYRGKAELHTAKTRKGFHLGFMDTSGGRLVDIERCEI
                     MEESINQQISEARSQERFEQSGSDLTFWSNPQGRAGDAVVRTVKSREFLVPRSGFFQA
                     NLYLTDAMVDEVCRLAGLEKRASILDACCGSGLFSIFLAQSAERLTGVEIHEKSVQYA
                     RENAERNGIQNAKFICADIGDVLSGMAERQDAVDLILLDPPRAGLDPKTLTAICKVKC
                     PDLIYVSCNPATQARDVKYLNECGYVLASLQPLDMFSQTEHIETVGWLRRK"
     gene            4064..4429
                     /locus_tag="PLA58_08725"
     CDS             4064..4429
                     /locus_tag="PLA58_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62914.1"
                     /translation="MEQRRVLEKKLELRLQKLEALGAAKEKDPMVKNLRSQIRETDIR
                     IAAFDKNIAKIEALKQAKAKKLAEKDAPKEAAAAPEKEAKPKKKAAAGEKEPKKKAAP
                     AEGEAPKKPKKKKEDAPAQ"
     gene            complement(4611..>5579)
                     /locus_tag="PLA58_08730"
     CDS             complement(4611..>5579)
                     /locus_tag="PLA58_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62915.1"
                     /translation="AMISQSGGNAAHCIQEGAARGVYFSKVISMGNGADLNESDFLEY
                     LTRDEETKIITAYLEGVRQGPRFFRALQKAAQSKPVVVLKVGTSESGAEAAVSHTTAL
                     AGSDQIWDGLLKQAGVIRAGSIEEMMDVALAFQQMPEPAGNRMVIVGIGGGASVILAD
                     EFSHAGLVLTRLGDDIRQKLINVFSTEAGRIFKNPIDLNNFETPDKFFGTMKILDACE
                     EADMLILHVAFDHFGLVSKTEKEFWVESYLKLIGRLKKELTKPLAVILHSYSSASMKP
                     LAQKAETELAAAGITVFPSIGRAALALSRFTRYHAKRAMKNPAGAR"
     assembly_gap    5580..5679
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5680..5927)
                     /locus_tag="PLA58_08735"
     CDS             complement(<5680..5927)
                     /locus_tag="PLA58_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR62916.1"
                     /translation="MNQSASLDFLFHPRSIAIAGVSENANKFNFGKRYLQGLIEFGFP
                     GKIYPLNPAGGEAEGIPLYKSLREIPGDVDYIISAIPAP"
     gene            6132..6872
                     /locus_tag="PLA58_08740"
     CDS             6132..6872
                     /locus_tag="PLA58_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62917.1"
                     /translation="MTNDLKEVIVSHLGNTVSAVGFAPVERFAQAPEQHHPAKICKNA
                     QTVIVLGLQIPRGILTSPDYNLYGLHRSYHTAYRRLDEIALELCNFIEAQGKYAAVMI
                     PSYAPMVFHNFEPWGILSLKHAAVRAGLGAFGRSGQVYHPVYGSRLRLAAVVTDAELP
                     GDPVNDRLPCPEKCRACLKICPAGAFTDEGDFRKMICLGHAIKHAIYPLALKDAAGLK
                     HIERVINTAGHDYWIDCDECMKVCPLNR"
     gene            6917..7348
                     /locus_tag="PLA58_08745"
     CDS             6917..7348
                     /locus_tag="PLA58_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62918.1"
                     /translation="MKRKMFFYAFLVLLVMAPVQLPAADFDWMPNFNVQAQADPSGFH
                     ARLATRFHIGDAQVNAVLSNFPKPADAYVALRLGEMSGKPIDVVTEQYKTKKGQGWGA
                     LAKSLGIKPGSKEFHALKRGDDLYGPQGKGKSKGKKDKKNK"
     gene            complement(7379..>7626)
                     /locus_tag="PLA58_08750"
     CDS             complement(7379..>7626)
                     /locus_tag="PLA58_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62919.1"
                     /translation="AASTKDQFAAEVKDLGHGVFTYTLLEGLKGKAAGGSDTVTVRKL
                     MGYIEEQLPEITKRYKQEAPFPVVDSRGMDFPLVIVR"
BASE COUNT         1747 a         2053 c         2148 g         1578 t
ORIGIN      
        1 aagctctcta ctcgcacacg gggccgctct ttcgcaggct gtataataca aaccggcttg
       61 caaattcaag cctgaaacgc taatacaccc gcatgatcaa aaaccagacc cctgaaatct
      121 ttttgaaaag cggcaggcag gccgcgcttt tgcgcgggca tccctgggtt ttttcgggtg
      181 cggtcgccaa aatacgcgga aacccgtccg acggcgagat cgttctggcc cgtgacacag
      241 gcggacaggc gctggccctg ggatttttca acagccgcac ggatatttcc ttccgtgtgg
      301 tcagccgcga tacgggcagc cccgtcgatg aatccttctg gaaccggaga gttctggacg
      361 cgctggaatt gcgcaggcaa atcatcaggg aggggaccaa ctgctatcgc ctgatcaatg
      421 ccgaaggcga cggtttcccc ggcctgatcg ttgatgttta caacgatacg ctggttttat
      481 ccgttacgac ggccgggatg gagcgtcaga agcagaccat tctggatgcc ctgcttttcc
      541 acctgaagcc ggcgcgcatc tacgagcaaa gcgccgggcg ctcgcgtggg ctggaaggcc
      601 tgcaggaacg caggggcttc ctccacggat ctgatcagga aggcgctgcg cgcgtgacgg
      661 aaaacggcca tcggtttgac gtggatttta tttccggcca gaaaaccggc tttttcctcg
      721 accagcgcgt caaccgggag acgattggtg ctctgtcccg gggcaggatg gtgctgaact
      781 gctttagcta caccggcgcg ttttccgttt acgctgccgc gggcaaagcc aaaaaagtgg
      841 tttctctgga tatttccaaa tccgcctgcg acgcggctgc ggagcatctg cgcatcaacg
      901 gctgcaaacc tgaagaccat ccggtcatcg aggcggatgt gtttgactat ctgcgaaatc
      961 tgcgggaatg tttcgatctg attgttctcg atccgcccgc gtttgccaaa ataaaaagag
     1021 acgtcgccaa agcctcccgc ggctacaagg atatcaacct gcaggccatc cggcatctgg
     1081 cgccgggtgg cctgctggca acgttttcct gctccaattt tatggaagaa gatctgttcg
     1141 gcaaaattgt tcagggcgcg gcgcgcgacg cgcaggccga cctgcaactg ctggcccgtc
     1201 tggaagcggg gccggatcat ccgctggcgg cgggccatcc cgagggccgg tatctgaaag
     1261 gactgctgct caggaaaccg gcatgacggg cgggaaaccg ggccgcgccg tccaaaagcc
     1321 ttttcgcgcc gtcagggcat gacgatattg gcggcgatct tgtcctgaat catccgcgaa
     1381 gcaatgttgc tgccgccgac gccgcacatc acggaaacat gggtcacatc cttttcaaca
     1441 tattcgacca ggatggtcac tttttcgtcc gcgatgacgg ccttgattga tccgctggcg
     1501 attttgcggt tcttttcgat cttgctgccc ttcagttccg tcacggtttt ttcactggcc
     1561 tgccatacct gatcgatatc ggccttgtac tgcgtggaca gatagccgtc ctggtagatg
     1621 aatcttccgg agctgacgcc catgactttt ccacccatga ccatggtggc gtcgcagccg
     1681 aacagcagaa aaaccgaaac gatcaggata tgcatctttg tcttcatgaa tttccctcct
     1741 gttaatcgga tatgtccgca tcataaagca aaggacatgg cgcgtcaaac gcgcgttaag
     1801 aatttgtaat aaaccgaaga tccggccgcc cagagcaaaa ccgcaacggt aatcagcacg
     1861 caggcgtacg caagaatttt gctccaggcg ctctccgact tctggtcgtt gtagcccatg
     1921 gcgtaagaca gcagcagtcc cgaaagaagt ccgccgccgt gcgcccagtt attgatgccg
     1981 ctgaagagaa atccgaagat gatcagtccc acgacccagc ccagcgactg cttgaaaatg
     2041 gcctctccgt aagagcctcc gcggcttttc ccgaaataaa tgatcgcgcc gatcagcccg
     2101 cagatggaag cggaagcgcc gatggtgaac tggacgcccg ccaggactga aacggcaaaa
     2161 ccgatgatgc cggtcaggat gtaaatgctc acgaaacggt ggagcccgaa ttcacgcagc
     2221 acgaaagggc cgagctggta gagcgccatc atgttgaaaa caatgtgcag gatgcccccg
     2281 tgcagaaacg aggcggaaac cacgctccag aagttgtggt aatacatgac gggaatggcg
     2341 ccgcttgccc cgagcgcgag caggctttga ttggaagggg agagaaacgc cagagggctg
     2401 cctcccgtga aaatgccccg cagatccatg agcagcgcca ggacgaaaaa tgcgatgttc
     2461 acgtacagaa tcgtctgcac cggctcaaac gcgaaaaaaa tattttgcag ttttccggcc
     2521 gtttcatgca ggccgggacg caccagtccg cagtaagggc aggccggctc atcgagactg
     2581 atcagcttgc ggcagttcgg gcagagaatg gaatttcttt gagcggacat tgggcattcc
     2641 ttaatgtttt cgtagaggga acccttaaca acaaaaaaag gtgttgtaaa gaaaaatgcg
     2701 ccggcgcggt tgactcccgc accgcaatat aggatagtgc cggacgtcag gaggatcatg
     2761 agcatcaaag aaagctgccg tttgaacatt acatccgttg cctttggcgg cgcaggcgtc
     2821 gggcgggccg acggcctggt gatttttgtg ccgttcaccg cagcggggga tgttgccgac
     2881 gttgaaatcg tcgagcgcag gaaaaaattc gcgcggggtc gcctgttggg catcctagag
     2941 ccttccccgc agagaacgga gccgctctgc cgttacttcg gccggtgcgg cggctgttcc
     3001 tatcagcaca tcgcttaccg tcatcagttg aacatcaagc aggcccaggt aaaagaggct
     3061 tttttgaaaa tcgggaaagt gccggagccg ccggttgaaa aagttatcgc gtcgccgtgt
     3121 gcctatgcct accggggcaa ggccgaactg cacacggcca aaacgcgcaa ggggtttcat
     3181 ctgggattta tggacacctc cggcggcaga cttgtggata ttgaacgctg cgagatcatg
     3241 gaggaatcga tcaatcagca gattagcgag gcaagatcgc aggagcgctt tgaacaaagc
     3301 ggcagcgatc tgaccttttg gtcgaatccg caaggccgcg cgggcgatgc cgttgtgaga
     3361 actgtgaaaa gccgggaatt tcttgtcccg cgctcgggat tttttcaggc caatttatat
     3421 ctgacggacg ccatggtgga cgaagtgtgc cgcctggccg gtttggaaaa aagagcgtcc
     3481 atcctggatg cctgctgcgg atcgggcctt ttttccattt ttctggcgca aagcgccgaa
     3541 cggctgaccg gcgttgaaat tcatgaaaag tccgttcagt acgcgcggga aaatgccgaa
     3601 cgaaacggga tacaaaatgc gaagtttatt tgcgccgaca tcggggatgt cttaagcggc
     3661 atggccgaaa ggcaggatgc ggttgacctt atccttctgg accccccgcg cgccggtctt
     3721 gatccgaaga cgctcaccgc catctgcaag gtgaaatgcc ccgatctcat ttatgtttcc
     3781 tgcaatccgg caacacaggc gcgcgatgta aaatatttaa acgagtgcgg atatgttctt
     3841 gcgagcctgc aaccccttga tatgttttcg caaaccgagc atatcgaaac ggtcggctgg
     3901 ctgcggcgga aatgaccggg atggatcgta gggaacggtc gcggccgttc cttaaaaaat
     3961 ggtggctttc tgatgaaaat attgctattt tccccggcaa ttcaagatat tttgtcaatt
     4021 ttcgagggag gatgagatgg catcaaaaag cagggaagtc agaatggaac aacgccgggt
     4081 actggaaaag aaactggaac tccggctgca gaaactggaa gcgcttgggg ctgccaaaga
     4141 aaaagacccc atggtgaaaa atctgagatc gcaaattcgt gaaacggaca tccgcattgc
     4201 cgcttttgat aaaaacattg caaaaattga agcattgaaa caggccaaag ccaaaaaact
     4261 ggcggaaaag gatgcgccca aggaagccgc cgccgcgccg gaaaaagaag ccaaaccaaa
     4321 gaagaaggcg gcagcgggtg aaaaggaacc caagaagaag gctgctccgg ctgaaggaga
     4381 ggccccgaag aagccgaaaa aaaagaaaga agacgccccg gcccaatagc gcctccgaaa
     4441 aaacgcaaac ggaatttttc ccgtgaagaa cgggacagag agttacggtt tattgccgta
     4501 actctttttt ttgaagcact gtgggcatac cgctaaatta taaattttcc tcagctgtgg
     4561 aaacgaatcg agggacaggt ttacaaaaag ttttttgctg cttcatccgg tcagcgggcg
     4621 ccggcaggat ttttcattgc ccgttttgcg tgatagcggg tgaaacggct cagggccagc
     4681 gccgcccgac cgatggaggg aaagacggtg attccggcag cggccagttc cgtttccgct
     4741 ttctgcgcca gcggcttcat gctcgcggag ctgtaactgt gcagaatgac cgccagcggt
     4801 tttgtgagct cctttttgag ccggccgatc aacttgagat acgactccac ccagaattcc
     4861 ttttccgtct tggaaacaag tccaaaatga tcaaacgcca cgtggaggat cagcatgtcg
     4921 gcctcctcgc aggcgtccag aattttcatg gttccgaaga atttgtcggg cgtttcaaaa
     4981 ttgttgaggt cgatcggatt tttgaaaatc cgcccggctt ccgtagaaaa cacattgatc
     5041 agcttttgcc ggatgtcgtc gccgagccgc gtcaatacga gtcccgcgtg agagaattcg
     5101 tccgcaagga tcacgcttgc cccgccccca atgccgacga tcaccatccg gtttcccgcg
     5161 ggctccggca tctgctgaaa cgccagcgca acgtccatca tctcttcaat gctgccggcg
     5221 cgaatgacgc ccgcctgttt caggaggccg tcccagatct ggtcggaacc ggccagggcc
     5281 gtcgtgtgcg aaaccgccgc ctcggcgccg gattcggacg ttccaacttt gagaaccacg
     5341 acgggcttgc tttgcgcggc tttttgcagg gccctgaaaa accgcggccc ctggcggacg
     5401 ccttccaggt aggccgtgat gatttttgtc tcttcatccc gggtcagata ttccaggaag
     5461 tcggactcgt tgaggtccgc gccgttgccc atgctgatca ctttgctgaa gtatacgccg
     5521 cgcgcggcgc cttcctggat gcagtgcgcg gcgttgcctc cgctttggga aatcatggcn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gggcgggaat ggccgaaatg
     5701 atgtaatcga cgtcgccggg aatctcccgg aggcttttgt agagggggat gccttccgct
     5761 tcgcccccgg cgggattcag cggatagatt tttcccggaa agccgaactc tataaggccc
     5821 tgcagatagc gcttgccgaa attgaacttg ttggcgtttt ccgacacgcc ggcgatcgcg
     5881 atggagcgcg gatgaaacag aaaatcgaga ctggcggatt gattcacgac gatcctcctg
     5941 aaatataggt gttgacgcca taacaccttt gctataattt gtacaccaat tatcagcctg
     6001 caagataccg ggatgatgcc cgtgactatc aaagtcgaaa acagcaatat tttctgccgg
     6061 cccgcgcccg gccgtcgggc gtgcgtttcc ggacgcagcc gcttcaaaac cggaaggaaa
     6121 aggaaaacga catgacaaac gacctgaaag aagttatcgt cagtcatctg ggaaacacgg
     6181 tgtcggccgt cggattcgcg ccggtggagc gtttcgctca agcgcccgaa cagcaccatc
     6241 cggcaaagat ctgcaaaaac gcacagacgg tgatcgtgct tggccttcag attccccggg
     6301 gaattctgac atcacccgat tacaatctgt acggcctgca ccgttcgtat cacacggcct
     6361 accggcggct cgatgaaatc gcgctggagt tgtgcaattt catcgaagcg cagggaaagt
     6421 acgccgccgt gatgattccc agctacgcgc cgatggtttt tcacaacttt gaaccctggg
     6481 gcatcctgtc gctcaaacat gccgccgtcc gggccggcct gggcgccttc ggccgcagcg
     6541 gccaggtgta ccatccggtc tatggctccc gcctgcgtct cgcggccgtg gttactgacg
     6601 ccgagctgcc cggtgatccg gtgaacgacc ggcttccctg tcccgaaaaa tgccgcgcct
     6661 gcctcaaaat ctgtccggcc ggcgctttta ccgacgaagg cgatttccgc aagatgatct
     6721 gcctgggtca tgccatcaag catgcgattt atccgctggc cctcaaggat gccgcgggtc
     6781 tcaagcatat cgagcgcgtc atcaacaccg ccggccacga ttactggatc gactgcgatg
     6841 aatgtatgaa agtctgcccc ctgaaccggt agggatgcgg gctgtgcttt tgacattttg
     6901 attcaaggag gatgatatga aaaggaaaat gtttttctat gcgttcttag tgttgctggt
     6961 gatggcgccc gttcagcttc ctgcggcgga tttcgactgg atgccgaatt tcaatgttca
     7021 ggcgcaggcg gacccgtccg gattccacgc ccggcttgcc acgcgttttc atatcggtga
     7081 tgcgcaggtg aacgccgttc tcagcaattt ccccaaaccg gcggatgcct atgtcgctct
     7141 gcgcctggga gaaatgtcgg gaaagccgat cgatgttgtc accgagcagt acaaaaccaa
     7201 gaaggggcag ggatgggggg cgctggccaa aagcctgggc atcaaacccg gctcaaagga
     7261 attccacgcc ctcaaacgcg gagatgatct ttacggtccg cagggcaagg gaaaaagcaa
     7321 agggaaaaaa gacaaaaaga ataaatagaa ttccgggcgg cagaagggaa gagcgcttct
     7381 atctcacgat caccagcggg aaatccattc cacgggagtc cacgacggga aacggggctt
     7441 cctgcttata ccttttggtt atttccggaa gctgttcttc gatatagccc atcagtttac
     7501 gcaccgtgac ggtatcactg ccgcccgcgg cttttccctt caagccttcc agcaaagtat
     7561 aggtgaacac cccgtgcccc aggtccttca cctcggcggc gaactggtct ttggtggatg
     7621 ccgcga
//
LOCUS       DAOSRM010000096         7440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_9287_length_7440_cov_107.143940, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000096 DAOSRM010000000
VERSION     DAOSRM010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9287_length_7440_cov_107.143940"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLA58_08755"
     CDS             <1..299
                     /locus_tag="PLA58_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR62920.1"
                     /translation="GNIRELENIIERGVAMETSNIILPENLALAADADTPGGPTELPL
                     PDTGIDLNAEMNRIEKRAIEKALRKTKGIKTKAAELLGVTGDSLKYRIEKLGIE"
     gene            319..864
                     /locus_tag="PLA58_08760"
     CDS             319..864
                     /locus_tag="PLA58_08760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HOR62921.1"
                     /translation="MIARLIEVRETKNRGRGVFAKEFIPKGTIICFECHQCRVLTADE
                     LGFYQMSEKEKMALLDWAYRKEDGTFVAPCDETRYLNHSCNANILGTDYGFDIVVENI
                     RKGEEATYDYRDFYDEVKMPCYCGEKNCCKVVDFKHPISEELRHFWAVKVNAALKLVN
                     QVSQPLKEELKKKSIFLPLHP"
     gene            924..1061
                     /locus_tag="PLA58_08765"
     CDS             924..1061
                     /locus_tag="PLA58_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR62922.1"
                     /translation="MKRGSPVNVATKGIKTKAAELLGVTGDSLKYRIEQLGMEQKKSA
                     F"
     gene            complement(1238..2842)
                     /locus_tag="PLA58_08770"
     CDS             complement(1238..2842)
                     /locus_tag="PLA58_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOR62923.1"
                     /translation="MSFADRNNPYSFNDFLAWRQGVDYYADDPFIQKVVRHFTGDLRQ
                     TVDAEARAVSRKASFRWRDMSERIARPEERPYMLHYDGHHNRIDRIVRPHDTEIMERE
                     IFSEAFFADKTSPWVKLIKMFIIYQNGEACIGCPVTCTEGLVELLAKYADSPETKEIL
                     RHCKEGIDGYFAIGAQYLSEIQGGSDVPSNVLEAVNENGIWKLYGTKFFCSATHADYA
                     VVTAKPVGSEKVGLFVVPSWLPGNKEKEIRNGCTIDRIKWKMGTSELTTAELTFQGAI
                     AYPVGPLDKGVANVVGIVLTYSRLTVGLSAAAYMTRAVREAEKYVSFRDAFGLRLNQF
                     PLVTVQLDRIRQTTRRTTAGAFKLYRDFLKLEGGLKGSQGGDEPLHARRNRFNVRELI
                     MLQKIAASWDCTDVIREAMSLFGGHGVMEDFSSLPRLYRDSAINELWEGPRNVLLTQI
                     HRDLQKAAGWYKPEEFVAEILQGLDPAQVREMAEEIQALVAHPHLFGMDEATIDICRR
                     WDAFCHKFTHAYQELAVKEMDLPVSE"
     gene            complement(2994..3269)
                     /locus_tag="PLA58_08775"
     CDS             complement(2994..3269)
                     /locus_tag="PLA58_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOR62924.1"
                     /translation="MKRAHVLISGRVQGVFFRAETLRTALSLGLAGWVSNREDGRVEA
                     LFEGEDESVDRMLAWCRQGPPHARVDRVDLAEEPWRGDLNGFRIKDF"
     gene            complement(3313..5373)
                     /gene="ligA"
                     /locus_tag="PLA58_08780"
     CDS             complement(3313..5373)
                     /gene="ligA"
                     /locus_tag="PLA58_08780"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOR62925.1"
                     /translation="MAGREDSPSKIMDKEAAHQRISELRKTIEYHSARYYQQDAPEIS
                     DAEYDRLMKELQNLEACYPDDELASSPTQRVGAAPLSKFAPFTHPQPMLSLANAFGAG
                     EIRDFDSRIRRLAKVERVDYVAEPKLDGLAVNLIYENGQFRRGATRGDGAVGEDVSQN
                     LKTIASLPLKMKTCASHTIPEFIEVRGEVYMEKEPFEKLNRRRVEEGEAPFANPRNAA
                     AGSLRQLDPKITARRPLNIFLYGVGSAGNAAFGSHWEIIQTLAAWGFPVNPLIRQASD
                     IDACIAYFEHIASIRSSLPYDIDGVVLKVNRLELQALLGSVSRSPRWALACKFPAEQA
                     ATVIQDIIVQVGRTGTLTPVAVMEPVNVGGVLVSRATLHNEDEILKKDIRVGDAVLIQ
                     RAGDVIPEVVSVILSKRTGREKEFRMPRACPECGSEIVRQAGEAAHRCVNLSCPAQLK
                     EHIRHFASRGAMDIEGLGEKLSAQLFDAKLINDPADLYFLTKEQLLGLDRQAEKSAEN
                     LLDAIERSRTPALDKFIFALGIRHVGERTAKLLAHHFGSLEALMSAGREELTAIHEIG
                     PEIAQSILEFFAEAKNRIVLEKFQRAGLSPQAPVPPAQTGLQGKSFVFTGSLESMGRN
                     EAKAVVESLGGVVHSSVTGKTSYVVAGREPGSKLEKARKQNIPVISEEEFLTMAGRPI
                     RRPA"
     gene            5492..5986
                     /locus_tag="PLA58_08785"
     CDS             5492..5986
                     /locus_tag="PLA58_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOR62926.1"
                     /translation="MKENKVRIGVLSDTHVYGFDRHLKQIVDEHFSDVDLIFHAGDLV
                     DLSVLELFGEKDVKAVFGNMDGRRVREQLPEQLTMDFGGFRVALIHGWGSPAGMERKL
                     ADRLGKVDCIVFGHTHYPVNRKIDGIYFFNPGSALDQRFAEHRSVGILEVGTEINGRI
                     IHLK"
     gene            6017..6502
                     /locus_tag="PLA58_08790"
     CDS             6017..6502
                     /locus_tag="PLA58_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOR62927.1"
                     /translation="METMCAPWRMEYLMCEKPEGCVFCKRSLRCEDYVLFDGKTCFVM
                     LNRYPYVNGHLMIIPIRHLGDIAGLTVGEREEIFSLVDISVKVLKEAMNPQGFNIGMN
                     LGKAAGAGIAEHVHVHVIPRWEGDTNFMSVVGNVRVIPQDLATTAATLIPLFEKYCPE
                     V"
     gene            6509..6826
                     /locus_tag="PLA58_08795"
     CDS             6509..6826
                     /locus_tag="PLA58_08795"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOR62928.1"
                     /translation="MKIFYLILTIVFAFFIVTFSQFNAAPVTIKYYYLQDLVVPAYML
                     IFVALLVGVVITGFLGVVERFRLNRTISKQNKVIRDLRKELREHEAPPMIEERKKTPA
                     PPA"
     gene            6823..7299
                     /locus_tag="PLA58_08800"
     CDS             6823..7299
                     /locus_tag="PLA58_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOR62929.1"
                     /translation="MTTETKFFADATLAGLARWLRLLGFDTVCFQGKAGRPLMRQAAA
                     EGRILLTRRRDLLERQFSGRLILLPGTTAVKQLSFALGKLSLEIQAQNLYTLCLSCNQ
                     KLISLERESVRDLVPEYVFEHCSVYTRCPQCRKIFWPGTHRRNALQFLKDNGFDVT"
BASE COUNT         1609 a         2006 c         2160 g         1665 t
ORIGIN      
        1 ccggaaatat ccgcgaactg gaaaacatca tcgaacgggg cgtggcgatg gagacttcca
       61 acatcattct gcctgaaaat ctggcgctgg cggcggacgc ggacacgccg ggagggccga
      121 ccgagctgcc ccttcccgat acgggcatcg atctgaacgc ggagatgaac cgcatcgaga
      181 aaagagccat cgaaaaggcg ctgcggaaga ccaagggcat caaaaccaaa gcggcggagc
      241 tgctgggcgt taccggcgat tccctgaaat accggatcga aaagctgggc attgagtagc
      301 ccgggaaggt gaaaaaacat gatagcgaga ttaattgaag tgcgagaaac aaaaaacaga
      361 ggcagaggag tttttgcgaa agaattcatc cccaaaggca cgattatctg ttttgaatgc
      421 catcagtgcc gtgtgctgac cgccgatgaa ctgggctttt atcagatgag cgaaaaagag
      481 aagatggctc ttttggactg ggcgtaccgg aaggaagacg ggacgtttgt cgccccttgc
      541 gatgagacga ggtacctcaa ccattcgtgt aatgccaata tcctcggtac cgactacggg
      601 tttgatatcg tggttgaaaa tatccggaaa ggcgaagagg cgacttacga ttaccgcgat
      661 ttttacgatg aggtaaagat gccctgctat tgcggcgaga aaaattgctg taaggtggtt
      721 gatttcaagc acccgatttc agaggagctg agacatttct gggcggtgaa agttaatgcc
      781 gcattgaaat tggtgaatca agtcagccag ccgcttaaag aggagctgaa gaaaaaatct
      841 atcttcctgc cgcttcatcc gtgagccggc agcaacagtt tctaagtgtg ggacagaggg
      901 cagagtggct gtaagccgaa aggttgaaga gaggaagccc cgtcaacgtt gcgaccaagg
      961 gcatcaaaac caaagcggcg gagctgctgg gtgttaccgg agattccctg aaataccgga
     1021 tcgaacagct gggcatggag cagaaaaaga gcgctttttg atcaaccgct gtttttccgg
     1081 cggttgagct ccgcggaata agcggcgatc aggtcgtcgt ggcgaagcac gcccaacaca
     1141 atattgccgt cgtcggggcg agaccggcat caaaggggcg atcagtccgc agccctgcgt
     1201 catctccagg cacatgagca tcaggccgat gccgagctta ctcactgacc gggaggtcca
     1261 tttccttcac ggccagctcc tggtatgcgt gggtgaattt gtggcagaag gcgtcccagc
     1321 gacggcagat atcgatcgtg gcctcatcca tcccaaacag gtgcggatgc gcaaccagcg
     1381 cttgaatttc ttccgccatc tcccgaacct gcgccgggtc cagtccctgc agaatttcag
     1441 cgacgaattc ctccggcttg taccagccgg ccgccttttg caggtcgcgg tggatctgcg
     1501 ttaaaagaac attgcgggga ccttcccaca gttcattgat ggccgaatcg cggtacaggc
     1561 ggggcagcga ggagaaatcc tccatcacac cgtggccgcc gaaaagcgac atggcttcgc
     1621 ggatgacgtc ggtgcagtcc caggaggcgg caatcttctg cagcatgatc agttcgcgaa
     1681 cattgaagcg gtttcgtcgg gcatgcagcg gttcgtcgcc gccctggctg cccttcaggc
     1741 cgccttccag tttcaggaaa tctcggtaga gtttgaaagc gccggcggtg gtccggcggg
     1801 tggtttgccg gattctgtcg agctggacgg tgaccagagg aaattgattc agccgaaggc
     1861 cgaacgcgtc gcggaatgaa acatattttt ccgcttcccg cacggcccgc gtcatgtacg
     1921 ccgcggcgga aagccccacc gtcagacgcg aataggtgag cacaatgccg accacgttgg
     1981 ccacgccctt gtccaggggg cccaccggat aggcgatcgc gccctggaag gtgagttcgg
     2041 ccgtcgtcag ttcgctggtg cccatcttcc acttgatccg gtcgatggtg cagccgttgc
     2101 ggatttcctt ttccttgttt cccggcagcc aggacggcac gacaaacagc cccacctttt
     2161 cggaaccgac aggcttggcc gtcacgacgg cgtaatccgc gtgcgtggcc gagcagaaaa
     2221 attttgtccc gtagagtttc cagataccgt tttcgttgac ggcttccaga acattggacg
     2281 gcacgtccga tccgccctgg atttcggaga ggtactgggc gccgatggca aaatagccgt
     2341 cgatgccttc cttgcagtgg cggaggattt ccttcgtttc cggcgaatcg gcgtatttgg
     2401 cgagcagttc gaccaggcct tccgtgcagg tgacggggca accgatgcag gcttcgccgt
     2461 tctggtaaat gatgaacatc ttgatcagct tgacccaggg agaggttttg tccgcaaaaa
     2521 atgcctccga aaaaatctct ctttccatga tttccgtgtc atggggccga acaatgcggt
     2581 caatgcggtt gtggtgcccg tcgtaatgga gcatataggg gcgctcctcc ggacgggcga
     2641 tccgctcgga catgtcccgc cagcgaaacg atgctttccg ggagacggcg cgtgcttcgg
     2701 catccaccgt ctgccggagg tcgccggtaa aatggcgcac gactttctga atgaacggat
     2761 catcggcgta gtaatccacc ccctggcgcc aggccagaaa gtcattgaag ctgtaggggt
     2821 tgtttcgatc tgcaaatgac atgtgtaaac ctcctcctgt tgtcttccgt aaaccggttt
     2881 gcccgttaaa gggttgtcag ggccgcgccc tgcgctgcta tatagaaccg gagtttgctt
     2941 cgtcaagttt tttccaattc gttcaggagg gcttttcggc agcgggttgc ggattaaaaa
     3001 tctttaatcc ggaagccgtt gagatctccc ctccagggtt cttccgccag gtccacgcgg
     3061 tccacgcgcg cgtgcggcgg tccctggcgg caccacgcca gcatccggtc cacgctttcg
     3121 tcttcacctt caaaaagggc ttcgacgcgg ccgtcttccc tgttgctcac ccatccggca
     3181 aggccgagac tcagcgcggt tcgcagcgtt tccgcgcgaa agaaaacccc ctgaacacga
     3241 cctgaaatca aaacatgcgc tcttttcatg acaaaccctt taaggggcgg ccaggcaaga
     3301 atggtccgtc tttcacgccg gccggcggat cggcctgccg gccatggtta aaaattcttc
     3361 ttcgctgatg acggggatgt tttgtttcct ggctttttcc agttttgacc ccggttctcg
     3421 gcccgcgacc acgtacgagg ttttgcccgt cacggaggag tggacgacgc cgcccagaga
     3481 ctcaacgacg gccttggcct cgtttctccc catgctttcc agggagccgg tgaaaacaaa
     3541 cgatttgccc tgcaggcccg tctgggccgg ggggaccggt gcttgggggg acaggccggc
     3601 ccgctgaaac ttttccagaa cgatcctgtt tttggcctcc gcaaaaaatt ccagaatgct
     3661 ctgggcgatt tccggtccga tttcatggat cgccgtcaat tcttcacgac cggcgctcat
     3721 cagggcttcc aggctgccga aatggtgggc cagtaacttg gcggtccgtt cgccgacgtg
     3781 gcggatgccg agcgcgaaga taaacttgtc cagcgcgggg gtcctggagc gttcaatggc
     3841 gtcaagaaga ttctccgcgg atttttccgc ctgccgatcc agccccagca gctgttcttt
     3901 cgttaagaaa tacaagtccg ccggatcatt gatgagcttc gcgtcaaaaa gctgagcgga
     3961 taatttttct cccaggcctt caatatccat cgcgccgcgc gaggcgaaat gccggatatg
     4021 ttctttcaac tgcgcgggac aggacagatt gacgcagcgg tgggcggctt caccggcctg
     4081 acggacgatt tccgaaccgc actccggaca ggcgcgcggc atgcggaatt ccttttcccg
     4141 ccccgttctt ttggacagaa tgacgctgac gacttccggg atcacgtctc ccgcgcgctg
     4201 aatcagaacc gcgtcgccca cgcggatgtc ctttttcagg atttcatcct cattgtgcag
     4261 cgtggcccgg ctgaccagaa caccgccgac attgaccggt tccatcacgg cgacgggcgt
     4321 cagcgttccc gttcttccca cctgaacgat aatgtcctgg atcaccgtgg ctgcctgttc
     4381 ggccggaaat ttgcaggcca gcgcccagcg gggagagcgc gatacgctgc ccagaagggc
     4441 ttggagctcc aggcggttga ccttcagcac gacgccgtcg atgtcgtagg ggagactcga
     4501 gcggatcgaa gcaatatgtt caaaataggc gatgcacgcg tcaatgtccg acgcctgccg
     4561 gatgagcgga ttgaccggaa atccccaggc ggcaagcgtt tggatgattt cccaatggct
     4621 gccgaaggcc gcattgccgg ccgaaccgac gccgtagaga aaaatattca aaggacggcg
     4681 ggcggtgatt tttggatcca gctggcgaag agatccggcg gcggcgttgc gcggattggc
     4741 aaaaggagct tcgccttcct ccacccggcg acggttgagc ttttcaaacg gttccttttc
     4801 catgtaaact tccccgcgga cctcgatgaa ttccgggatg gtatgagagg cgcaggtctt
     4861 cattttcagg ggcagggaag caatggtttt gaggttttgc gaaacgtctt cgcctacggc
     4921 gccgtcgccg cgcgtcgcgc cgcggcggaa ctgtccgttt tcgtaaatca gatttacggc
     4981 caggccgtcc agtttgggct ccgccacgta atccacgcgc tcaaccttcg ccagccggcg
     5041 gatgcgcgaa tcgaagtccc tgatttctcc ggcgccgaat gcgttggcca ggctgagcat
     5101 gggttgaggg tgcgtgaagg gggcaaattt ggaaagcggg gcggcgccga cgcgctgcgt
     5161 cggagacgag gccagctcat cgtccggata gcaggcttcc aggttctgca gctccttcat
     5221 caggcggtcg tactcggcgt cggatatttc cggcgcgtcc tgctggtagt agcgcgcgct
     5281 gtgatattcg atggttttgc gcagttcgga aatgcgctgg tgagcggctt ccttgtccat
     5341 gattttactt ggagaatctt cccgtccggc catgattcga attctagccc gccttttgat
     5401 caaagtcaat tcaaaagcac gcgggcggcc gttctttgat aaataacact tgtaattttt
     5461 tcgtctaccc gctaagaaat ccagcaagat catgaaggaa aataaagtca ggataggcgt
     5521 gttgtccgac acgcatgttt atggttttga tcggcacctg aagcaaattg tcgacgagca
     5581 tttttctgat gttgacctga tttttcatgc cggcgatctg gtggatttat ccgttctgga
     5641 gcttttcggc gaaaaggacg tgaaggcggt tttcggcaat atggacggcc ggcgggtcag
     5701 agaacagctg cccgaacagc tgacgatgga ttttggcggg tttcgcgtcg cgctgattca
     5761 cggatggggg tcgcccgcgg gcatggaaag aaaactggcc gacaggctgg ggaaggtgga
     5821 ttgcatcgtt ttcggccata cgcattaccc ggtcaacagg aaaatcgacg gcatttattt
     5881 tttcaatccg ggttccgcgc tggatcagcg gtttgccgaa caccgcagcg tcggaatcct
     5941 ggaagtgggg actgaaatca acggcagaat tatccatctc aaatagcggg attcaaagag
     6001 atcggggatt gaaacaatgg aaacgatgtg cgcgccgtgg cgaatggagt atctcatgtg
     6061 tgaaaaaccg gaaggatgcg tgttttgtaa aaggtctctt cgctgcgaag attatgtttt
     6121 gtttgacgga aagacctgct ttgtcatgct gaaccgttac ccgtatgtca acggccattt
     6181 gatgattatt ccgattcgcc atctgggcga cattgccgga ttgaccgtgg gggagaggga
     6241 agaaattttc agcctggtgg acatctccgt caaagttctg aaggaggcga tgaatcccca
     6301 gggctttaac atcggcatga atctgggcaa agcagccggc gcggggattg ccgaacatgt
     6361 ccatgttcac gtcattcccc gctgggaagg cgataccaat ttcatgagcg tcgtcggcaa
     6421 tgtccgcgtc attcctcagg acctggccac caccgccgcg acattgattc ccctgtttga
     6481 aaaatattgc ccggaggttt aaaacgcgat gaagatattc tatttaatcc tcaccattgt
     6541 ctttgctttt tttatcgtga ccttttcaca attcaacgcg gcgccggtga ccataaaata
     6601 ttattacctt caggatctgg tcgttccggc ctacatgctc atttttgtcg ccctgctggt
     6661 gggcgttgtc attaccggtt ttttgggcgt tgtggaaaga tttcggctca accgcaccat
     6721 ttccaaacaa aacaaggtga ttcgcgattt gcgcaaagag ctgagggaac acgaagcccc
     6781 tccgatgatt gaggaacgca agaaaacacc cgctcctccg gcatgaccac agaaacaaaa
     6841 ttttttgccg acgcaacgct tgccggtctg gcccgatggc tcagactgct gggctttgat
     6901 acggtctgct ttcagggaaa ggcgggccgg ccgttgatgc ggcaggcggc ggccgaaggc
     6961 cggattttgc tcacccgaag aagagacctc ctggagcggc agttttccgg gcgtctgatc
     7021 ctgctgccgg gaaccaccgc ggtgaagcag ctttcttttg cgctcgggaa gctgtcgctg
     7081 gaaatccagg ctcaaaacct ttacacgctt tgtctttcct gcaatcaaaa actgatttcc
     7141 ctggagcggg aaagcgttcg ggatctggtg ccggaatatg tttttgaaca ttgttcggta
     7201 tatacccggt gcccccaatg ccggaaaatc ttctggccgg gaacacaccg gcgaaacgcc
     7261 ctgcaatttt tgaaagacaa cggctttgat gtgacctaaa ccggaaaagc caaaaccaaa
     7321 acatccagtc ggcgcatcga tattctggat tagagactcc tctcaccctc ccccggcccc
     7381 tcccatcgag agctttgaat aacggccgtt tttgtcattt cgaccgaagg gagaaatctt
//
LOCUS       DAOSRM010000097         7393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_9358_length_7393_cov_114.011038, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000097 DAOSRM010000000
VERSION     DAOSRM010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9358_length_7393_cov_114.011038"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="PLA58_08805"
     CDS             complement(<1..1296)
                     /locus_tag="PLA58_08805"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOR62930.1"
                     /translation="MEQAQAKTTAVKGTDTAREVRALVKNIYKSAHEAKDQGKKVAYI
                     MVASQYDEILRAMDIVPLPTENYAGLCAAKRDMDRFLLKAEADGYSQVLCSYARIGLG
                     FDSLRKELGRIPEGSPDGGMPVPDMMLGSSAVCDPRFKWYQATSRYLEVPTFGIDVVA
                     PPPQADLNAVRDYYIQYQRDQLKGLIGFLEAQTGTKMDPDRLWECIRLGDRAWQLWYD
                     IDRLRVAIPCPMPSQDHFSIMVAGHYYTGTQIAVDFYRKLYDEVKDKVDRKIGVIPDE
                     KHRILYGGGLPPWHTLWIFNYFESFGAVFVIENVYRGFDPVEVPSFVKDPVDFLAWRA
                     FLRFTRYHEKARARSGNPIVEKLLDMIEDYKIDGVVFHACRSCRATTIGQTHIKSELG
                     KYSDLPMMQLVSDMVDLRDYSEAQWKAQIGAFIEALQGRG"
     gene            complement(1344..1898)
                     /locus_tag="PLA58_08810"
     CDS             complement(1344..1898)
                     /locus_tag="PLA58_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62931.1"
                     /translation="MKDRKKQRLPGFNYSTDGAYFVTVCVQNMQYCFGNVENETMALN
                     QTGKIVDHQWNWIFEQYAYVHRDEFVVMPNHVHGILHIAENGLATRTGGRNLDVGNGR
                     DRSLQAQKIKTISELIGAFKTTSSKMIHLAGQTDFKWQKSYYDRVLRNERELHLTREY
                     IVNNPKAWELDKNNPDINGNKLFL"
     gene            complement(1959..3098)
                     /locus_tag="PLA58_08815"
     CDS             complement(1959..3098)
                     /locus_tag="PLA58_08815"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOR62932.1"
                     /translation="MTVTKPIEELYHQRGSRARALHDAGKKVIGYFCCFVPDEILTAF
                     DLVPYRIQGSQSEPIDEADALLEPMACPFARSCFNLALKGRYDFLDGFVAPHSCDTIE
                     RMYHIWHSNKPSPFNHLINVPHMMGPSSDAFYRKELEYFIARLEEFTGRKLDLEKLRS
                     AIRLYNRRRTLLRELYDLRRENPPRVSGTEITKVLVAGMGIPAAEHIELVSAFIDEVK
                     KRPAPAPDGLPRIFLWGNEIDDIAFVSLVEECGAHVVMDDLCTGSRFFWQDVPETDDP
                     VDALARRYLSTHCPRSLKPQAGNRSEDLENRFGYIREFISKWKADGAIFYIVRYCDTC
                     ELEGPDLREYLTGLKLPVLMIEDDYSTSTIGQLRTRIQAFLEMIM"
     gene            3244..4062
                     /locus_tag="PLA58_08820"
     CDS             3244..4062
                     /locus_tag="PLA58_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62933.1"
                     /translation="MQKTIIQIYEVQKPGEAEALIELGVDHIGSVLADPAKWKSASIR
                     KTVQTVKRRGAKSVLIPLMKDPAAIFAALDYYEPDCVHLCDVLSPYPDEQQTVVRDFD
                     ALLSLHIDIRDRFPRLDIMRSLSVPRAGLPDASVIEKNILDFAARFAPVTDFFLIDTL
                     LSDADQPVAGFVGITGKPCDWTIAKAVIEQSPISVILAGGLAADNVYDAVVGLRPAGV
                     DSCTRTNAADRSGRPVRFKKDLKKVRGFIEEVRRADRFLEGESRDAKLIHRERI"
     gene            4028..4363
                     /locus_tag="PLA58_08825"
     CDS             4028..4363
                     /locus_tag="PLA58_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOR62934.1"
                     /translation="MTRNSSIANEYKTLSAMMALYCRERHAAGGKELCPSCAELLTYA
                     KSRLDKCPFAADKPACSACPRHCYSPSRRRQIQEVMRCAGPRMLRSHPLLAIRHLLKK
                     FKKPGGRKS"
     gene            4360..5475
                     /locus_tag="PLA58_08830"
     CDS             4360..5475
                     /locus_tag="PLA58_08830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR62935.1"
                     /translation="MKMAIIGAGPGGLYAALAAVRKNIKVDLFEKRKVGEGIVCGECI
                     FDSLGIMARPGRGLLHPVEGLVLKGSGEYRFPLGRHKPLWMLDRRVWQRDLSRQAQEL
                     GVRLHENARVTVSDLARMKKSYQWILDASGAPSVASRLYGFTREYIQECLAAHQVRLA
                     GDFSPLVPCIRVVFFPDLPAENQPGYAWVFPRDAESANVGVVCTVRKEPGHGRPDMKK
                     MLTDFLRREGLEGSPVLERGGGIAPVRMLSRLVYDNILLVGDAAGLTSALHGGGIDMA
                     CLSGVLAVQAVCEGQAGAARYAGALRRYSREKNALEKVTIGKMRALNFDRFDRLLFGV
                     TSPSLRVRFWTALRNPVMFWTTIRWFTAKKKIPDWPV"
     gene            complement(5502..>7393)
                     /locus_tag="PLA58_08835"
     CDS             complement(5502..>7393)
                     /locus_tag="PLA58_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOR62936.1"
                     /translation="GTGVPLAAALLYIAGVKPLGGLNILPFAYILSSLTAAWIILGQK
                     LFGLRPVAHGTIFENMTDGFVVLNKELKIVDANPPALRLLNLSPEALGQFGNRAFVRH
                     PSLIRLIHSEPHGHVQIEQDGRIFDAFRASLTDQQKNLAGHLISLRDVTSIKRAENAL
                     RQSELRFRQISHLTSDILYSCTTDQDGNFAIDWISGNTAAVTGYSIEEIKAQSCWRFL
                     VTEEDLSLFEENVTGLDPGQSRTADLRLRHKNGKTVWVVSHAECVREPQDPETIRLYG
                     GLVDITSRKRAEEELLKNESQYRFLTENMNDVLWMMDLNLRTVYVTPSIEAVLGFTQE
                     ERLRQSVEEQLTPESLRIARETLARERVFEGQPGFRTRKATLTLEFYHRDGSTRRLET
                     IVSGLFDDQGRLTGLYGVSRDVTERKETETRILQYSEALRERNKELHCLYSVSELVRK
                     EEISQEDILSASASLIAQSYFYPEIAGCRIAWEDREYKTENFAPTSWMQTSAIRVRGK
                     EVGKIDVCYLELRPEKDEGPFLKEERKFLDGIAGLLGKSYERRLAVGEREKLIGELQN
                     ALQEVKTLTGMLPICASCKKIRNDQGYWEKIERYIGERSSVRFSHGICPECAQKLYPD
                     LNLKK"
BASE COUNT         1442 a         2101 c         2198 g         1652 t
ORIGIN      
        1 ccctctcccc tggagggctt cgataaacgc gccgatctgc gccttccact gcgcctccga
       61 gtaatcccgc aaatccacca tgtcggagac gagctgcatc atcggcagat cggaatactt
      121 gcccagttcg cttttgatgt gcgtctggcc gatggtggtg gcccggcagg accggcaggc
      181 gtgaaagacc acgccgtcga ttttgtagtc ttcgatcatg tccagcagct tttccacgat
      241 gggattgccg ctgcgcgcgc gggccttttc gtggtagcgc gtgaagcgca gaaatgcccg
      301 ccaggcgagg aaatccaccg gatccttgac gaaagagggc acttccaccg gatcgaagcc
      361 gcggtaaacg ttttcgatca cgaacaccgc gccgaagctt tcaaagtagt tgaaaatcca
      421 cagcgtgtgc cagggcggga gcccgccgcc gtaaaggatg cggtgttttt cgtccggaat
      481 gacgccgatt ttccgatcca ccttgtcttt cacttcgtcg tagagcttcc ggtagaagtc
      541 cacggcgatc tgcgttcccg tgtagtaatg gccggcgacc atgatgctga agtgatcctg
      601 cgacggcatc gggcagggga tcgccacacg cagccggtcg atgtcgtacc agagctgcca
      661 ggcgcggtct cccaggcgaa tgcactccca gaggcggtcg gggtccattt ttgtgccggt
      721 ctgcgcctcc agaaatccga tcaggccttt gagctggtcg cgctgatatt gaatgtagta
      781 gtcgcgcacg gcgttcaggt ccgcctgggg cggcggggcg accacgtcga tgccgaaggt
      841 gggaacttcc agatagcggc tggttgcctg ataccatttg aagcgggggt cgcacaccgc
      901 ggagctgccc agcatcatgt cgggcacggg catgccgccg tcggggcttc cttcggggat
      961 gcgccccagc tccttgcgca ggctgtcgaa gcccaggccg atgcgggcgt agctgcagag
     1021 aacctgcgag tagccgtcgg cttcggcttt cagaagaaag cggtccatgt cgcgcttggc
     1081 ggcgcacagg cccgcgtaat tttccgtggg cagcggcacg atgtccatgg cgcgcagaat
     1141 ttcatcgtac tgcgaggcca ccatgatgta ggccaccttt ttgccctggt ctttggcctc
     1201 atgggcgctt ttgtaaatgt ttttcacgag cgcgcgcact tcccgcgccg tgtccgtccc
     1261 cttcaccgcg gtcgtcttgg cctgtgcctg ttccataaaa gttaccccta gatttgtctg
     1321 tagggaacgg tcgcgaccgt tccctacaaa aacaatttgt ttccgttaat atccggattg
     1381 tttttgtcca actcccacgc ttttggatta ttgacgatgt attctcttgt taaatgcagc
     1441 tccctttcat tgcggagtac tctgtcatag taagactttt gccatttgaa atccgtttgg
     1501 cccgccaggt ggatcatttt tgacgaggtt gttttaaatg cccctattaa ttcagagatg
     1561 gttttaatct tctgcgcttg tagggaacgg tcgcgaccgt tccctacatc caagttgcgt
     1621 ccacccgtgc gggtggcaag accgttttct gcgatgtgaa gaatgccgtg aacatgattg
     1681 ggcatgacaa caaactcatc gcgatgaaca tacgcatatt gttcaaaaat ccagttccac
     1741 tgatggtcca cgattttgcc cgtttgattt aaagccatgg tctcattctc gacgttgccg
     1801 aaacagtact gcatattctg cacgcagacg gtgacaaaat atgcgccgtc tgttgaataa
     1861 ttgaatcccg gcagacgttg tttcttcctg tctttcataa cgcctcaaag gcatggaacg
     1921 gtcgcgaccg ctccctgcat ccaagtcgcg acatgcccct acatgatcat ttccaggaac
     1981 gcctggatgc gggttctcaa ctgcccgatg gtcgaggtcg agtagtcatc ctcgatcatc
     2041 agcaccggca gcttcaggcc ggtcaggtat tcccggagat ccgggccttc cagctcgcag
     2101 gtgtcgcagt agcggacaat gtaaaagatc gccccgtcgg ctttccactt gctgatgaat
     2161 tcccggatgt agccaaagcg gttttccaga tcctcactgc ggttgccggc ctgcggcttc
     2221 aggctgcggg ggcagtgcgt cgagaggtag cgccgggcga gcgcatccac cggatcgtcc
     2281 gtttccggga catcttgcca gaaaaaccgc gacccggtgc acaaatcgtc catcacgacg
     2341 tgcgcgccgc attcctcaac caggctgaca aaggcaatgt cgtcgatttc gttgccccac
     2401 aaaaagatgc gcggcaggcc gtccggcgcg ggcgccgggc gctttttcac ctcgtcgatg
     2461 aatgccgaaa ccagttcgat gtgctccgcg gcggggatgc ccatgccggc caccaggact
     2521 ttggtgattt ccgtgccgga aacgcgcggc gggttttcac gtcgcagatc atagagctcc
     2581 cgaagcaggg tgcggcgccg gttgtagagc cggatcgcac tcctgagttt ttccaaatcc
     2641 agtttccggc cggtgaattc ctccagacgg gcaataaagt attccagctc tttgcggtaa
     2701 aaggcgtcgg aagacgggcc catcatgtgc ggcacgttga tcagatggtt gaaaggggag
     2761 ggtttgttgc tgtgccagat gtggtacatg cgttcgatgg tgtcgcagct atgcggggcg
     2821 acaaacccgt cgagaaaatc ataccggccc ttgagcgcca ggttgaagca gctgcgggca
     2881 aacgggcagg ccatcggttc gagcagcgcg tccgcctcgt cgatgggttc gctttgcgag
     2941 ccctggatgc gatagggcac caggtcgaag gccgtgagaa tttcatccgg cacaaagcag
     3001 cagaagtagc cgatgacttt tttccccgcg tcgtgcagcg cgcgcgcgcg ggagcctctt
     3061 tggtgataca gctcctcgat gggtttggta accgtcataa acgcccccct ttgtcctctc
     3121 tggatggccc agattattca gtgaattgcg gataaaagca agttgctttt ttgaaaatat
     3181 cagggcgggc gcacccccgt ctgtgatctt gatcgaaggc gggggtcgtg ataaggcgtt
     3241 ggcatgcaaa aaacgatcat tcagatttat gaagtgcaaa agcccgggga agccgaagcg
     3301 ttgatcgaac tcggcgtgga tcatatcggc agcgtgcttg ccgatccggc aaaatggaaa
     3361 agcgcatcca tccggaaaac cgtgcagacg gtcaagcgcc gcggcgcgaa aagcgttttg
     3421 attccgctca tgaaagaccc ggcggccatc tttgcggcgc tggactatta tgagccggat
     3481 tgcgttcacc tgtgcgatgt gctttcgcct tatcccgatg aacagcagac ggtggtgcgc
     3541 gacttcgacg cgcttttgag cctgcacatc gacatccggg accggtttcc gcgcctcgac
     3601 atcatgcggt ctctgtccgt gccccgggcc ggattgcccg acgcctccgt catcgaaaaa
     3661 aacatcctgg attttgccgc gcgttttgcg ccggtgacgg atttctttct gatcgacacc
     3721 ctgctttcgg acgccgacca gccggtggcg ggctttgtcg ggatcacggg gaaaccctgc
     3781 gactggacga tcgccaaagc cgttatcgaa caaagtccca tttcggtgat tctggcggga
     3841 gggcttgccg ccgacaacgt ctatgacgcg gtggttggcc ttcggccggc gggggtggac
     3901 agctgcacaa ggaccaatgc ggccgaccgg agcggacggc ccgtgcgctt caaaaaagat
     3961 ctgaaaaaag tccgggggtt cattgaagaa gtgcgccgcg ccgaccgttt tcttgaagga
     4021 gagtcccgtg acgcgaaact catccatcgc gaacgaatat aaaacgcttt cggcgatgat
     4081 ggccctgtac tgccgggagc gccatgcggc aggcggcaag gaattgtgcc cgtcctgtgc
     4141 agagcttctg acctacgcaa aatcgcgtct ggacaagtgt ccgttcgccg cggacaagcc
     4201 ggcctgttcg gcctgtccgc ggcactgcta cagcccatcc cgccggcggc agattcagga
     4261 agtgatgcgc tgcgccggtc cgcgcatgct gcgctcccat ccgctgctgg cgattcgcca
     4321 ccttctgaaa aagtttaaaa agcccggagg acgcaagtca tgaagatggc gatcatcggc
     4381 gccggtcccg gcggtctcta cgctgcgttg gcggcggtcc gcaaaaacat caaagtggac
     4441 ctgtttgaaa aaagaaaagt cggggagggc atcgtctgcg gcgaatgcat ttttgattcg
     4501 ctgggaatca tggccagacc ggggcgggga ttgctgcatc ccgtggaagg gcttgttttg
     4561 aaaggcagcg gcgagtaccg ctttcccctg ggcaggcata agcccctgtg gatgctcgat
     4621 cggcgggtct ggcagcggga cctttcccgt caggcgcagg agctcggcgt gcgcctgcat
     4681 gaaaatgcgc gggtaaccgt gtcggatttg gcgcggatga agaaatcgta ccaatggatt
     4741 ctggacgcca gcggcgctcc gtctgtggcg tcccggcttt acggatttac acgcgagtat
     4801 atccaggagt gcctggcggc gcatcaggtg aggctggccg gcgatttcag tccgcttgtg
     4861 ccctgcattc gggttgtgtt ttttccggat cttccggccg aaaatcagcc cggctacgcc
     4921 tgggtttttc ccagggatgc cgaaagcgcc aacgtcggcg tggtctgtac ggtgcgcaaa
     4981 gaacccggac acggacgacc ggatatgaaa aaaatgctga cggatttttt gcgccgcgaa
     5041 ggacttgaag gcagccccgt tctggaaaga ggcggcggca ttgcccctgt gcggatgctt
     5101 tcccgtctgg tttacgacaa tattcttctg gtgggcgacg cggcggggct cacctccgcg
     5161 ctgcacggcg gcggcattga catggcctgc ttaagcgggg tgctggccgt gcaggccgta
     5221 tgcgaagggc aggccggcgc agcccgctat gcgggagcgc tcagacgcta cagtcgggag
     5281 aaaaacgccc tggagaaagt gaccatcgga aaaatgcgcg cgctgaactt cgatcggttc
     5341 gatcgactgc tcttcggcgt gacctcccca agcctgcgcg tccgcttttg gaccgctttg
     5401 cgcaatccgg tgatgttctg gacgacgatc cgctggttta cagcgaaaaa gaaaatcccc
     5461 gactggccgg tttgaacctc cgttcccggc gactcgggcg cttatttttt aaggttcagg
     5521 tccggataca atttctgcgc gcactccgga caaattccgt ggctgaaccg gaccgacgag
     5581 cgttctccga tgtaacgctc gattttttcc cagtatccct ggtcgtttcg tatttttttg
     5641 cacgacgcgc agatcggaag catgcccgtc agggtcttga cctcctgcag ggcattttga
     5701 agttccccga tgagcttttc ccgctctccg acggccagcc gccgttcgta cgattttccc
     5761 agcagcccgg cgatgccgtc aaggaacttg cgctcttctt ttaaaaaagg gccctcgtct
     5821 ttttccggcc gcagctcaag gtagcagacg tcgattttcc caacctcttt gccgcgcacc
     5881 cggatggcgc tcgtctgcat ccaggaggtc ggggcgaagt tttccgtctt gtattcccgg
     5941 tcttcccacg caatccggca gcctgcgatc tccggataaa aataggactg cgcgataagg
     6001 gaggcggacg ctgaaagaat gtcttcctga gagatttctt ccttccggac cagctccgac
     6061 acggaataga gacaatgaag ctccttgttt ctttctctga gcgcctccga atactgcagg
     6121 attctggttt ccgtttcctt gcgttcggta acgtccctcg agacgccgta gagaccggtc
     6181 agtctgccct gatcgtcaaa gaggccgctg acaatcgtct ccagccggcg ggtggatccg
     6241 tcccggtggt agaattccag tgtcaacgtc gctttccttg tgcgaaagcc tggctgtcct
     6301 tcaaagactc tttcccgggc cagcgtctcc cgggcgatcc tgagcgattc gggcgtcagt
     6361 tgttcctcaa cgctctgacg aaggcgttcc tcctgagtga agccgagaac ggcttcgatg
     6421 gagggggtga cataaacggt ccgcagattc agatccatca tccacaggac gtcgttcatg
     6481 ttttcggtca gaaaccggta ttggctttcg ttttttagaa gttcctcctc cgcccgcttg
     6541 cgggaggtga tgtccaccag tccgccgtag aggcggatcg tttccgggtc ttgaggctcc
     6601 ctcacacact cggcgtgaga aaccacccag accgttttgc cgttcttgtg gcggaggcga
     6661 agatcggcgg tccgcgactg gcccgggtca agcccggtca cattttcctc gaaaagcgaa
     6721 agatcttcct ccgtcaccag gaagcgccag caggactgcg ccttgatttc ctcgatggaa
     6781 tagcccgtga cggccgcggt gttgccggat atccagtcga ttgcgaagtt tccgtcctgg
     6841 tcggttgtac aggagtagag gatgtccgag gtcaggtggg aaatctggcg gaaccgcagt
     6901 tcgctttgcc gcagagcatt ctctgcccgc ttgatggagg tgacgtcgcg cagactgatc
     6961 aaatggccgg ccagattttt ttgctggtcg gtgagagagg ctctgaacgc gtcgaagatc
     7021 cgtccgtcct gttcaatttg cacgtggcca tgaggttccg aatgaatcag ccggatcagc
     7081 gacgggtgac ggacaaacgc cctgttcccg aattgaccca gcgcctccgg cgaaaggttc
     7141 agcaggcgca gagcaggggg attggcgtcc acaattttaa gttccttgtt cagcacaaca
     7201 aatccgtcgg tcatgttttc gaagatcgtt ccgtgtgcga cgggccgcag accgaaaagc
     7261 ttttgaccca ggatgatcca cgcggccgtc agactggaaa gaatgtaggc aaaggggagg
     7321 atgttcaatc ctcccagagg tttcacgccg gcgatataaa gcagtgcggc cgccagcgga
     7381 acacccgtcc cga
//
LOCUS       DAOSRM010000098         7244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_9595_length_7244_cov_116.679232, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000098 DAOSRM010000000
VERSION     DAOSRM010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9595_length_7244_cov_116.679232"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..612
                     /gene="rimP"
                     /locus_tag="PLA58_08840"
     CDS             127..612
                     /gene="rimP"
                     /locus_tag="PLA58_08840"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOR62937.1"
                     /translation="MYDELKEKIWQLAEPVVLSEGMELIHVECVKMHSRWVFRFFLDK
                     EGGVTLDDCTNVSNQLGDILDVHDLSRGPYTLEVSSPGLDRPISRDQDFEKFLGAKVK
                     IRTHGKIEGIRNFQGTLADYTEEDGRKVVRIDMADKRYSIPKDEIAKANLVDEDTAIA
                     E"
     gene            675..1937
                     /gene="nusA"
                     /locus_tag="PLA58_08845"
     CDS             675..1937
                     /gene="nusA"
                     /locus_tag="PLA58_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOR62938.1"
                     /translation="MLQDLKRLIEQMGKDRGIDKQIITEALEAAMLTAARKKLGQDVD
                     IEAHYNDEAGEIEVFQFKTVVDKVLDPETQIAHEEARRTLDEGAEPGDSLGMKIETSS
                     FGRIAVQMAKQIIIQRVKDAERDNIYDEYKDRKGELINGFVQRFEGGNIIVNLGRAEG
                     VVPPMEQIHRETYKRGERIRSYLFEVKKNTKGAQIILSRTHPAFLKALFEVEVPEISE
                     GLIEIVSVAREPGKRGKIAVRAKDKDIDPVGACVGMRGTRVQSVVQELRGEKIDIIPY
                     TDDAAKFVSSALSPAKVNRVLIDEENRAMTVIVPDDQLSLAIGKNGQNVRLAVKLTGW
                     KIDVKSETMAAEKDEDAYASLTRVPGIGPAMAEKLFAQGIKSAAMLAAMEPETLSAIP
                     GVGEKTATEWIAEAIRLLDEETSDRQQG"
     gene            1982..4576
                     /gene="infB"
                     /locus_tag="PLA58_08850"
     CDS             1982..4576
                     /gene="infB"
                     /locus_tag="PLA58_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOR62939.1"
                     /translation="MSKKRVHELAKELGLENKDLIAHLERLGITVKSHASTLEDHEIE
                     RIREDLQATSPRQVVEERIKTTVIRRRTVRTPVEETPEEKEEKPEAEKAEREAAGAKK
                     AKLEEQPAAAAVEGERKPAPAEAAKPTVPESPVLPEKEPAVEAKPEQPTPAAPPVKPK
                     APQVILPVRPPMMSRHKIVRAEEKKEIPAKAPARPGEALAPPAPEKIVKKEFEKPKKK
                     GKGTVEVLIGDEKEVPRRKILERKIDKKLKRVDDDREVVFSKWREDRKSAPVKMKKTE
                     ITVPKAIKRRLRVGDTITTGELAKRMGVKAGEVINKLMGMGVMATINQSIDFDIASLV
                     ASEFSFQVERAEMEFEDSMLKPQASGENLKPRAPIVTIMGHVDHGKTSLLDAIRQTHV
                     IDGEAGGITQAIGAYHVHINDRDIVFLDTPGHEAFTAMRARGAQVTDIVVLVVAADDG
                     VMDQTVEAINHSRVAGVPIIVAINKIDKPGANPDQIKQALAEHGLLSEQWGGDTIFTE
                     VSAKKKIGIEELLEMILLQADVLELKADPDQAARGIIIEAKLDRGRGPVATVLIQQGT
                     LREGDAFVSKTESGRVRAMINDQGRRVKEAGPAMPVEVIGFSSVPQTGAEFISVEDEK
                     KARNIADYWVRKTREKELSASSKITLEQLYQKIKEGVKDLNVIVKADVQGSIEAISDA
                     FKKLSTEDVQIKMIHSSTGAISETDIMLASASNAIVIGFNVRPDSRVSDLAGQEGVEI
                     KLYDIIYNVIADMKAAMEGLLEPEYREVVQGRAEVRELFRVPKVGIIAGCHVTDGKIT
                     RTAGIKLVRDSVAVFDGKILSLKRFKDDAREVLAGFDCGIGIESYNDIHVGDVIESYI
                     TETLERKL"
     gene            4589..4927
                     /locus_tag="PLA58_08855"
     CDS             4589..4927
                     /locus_tag="PLA58_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOR62940.1"
                     /translation="MIIGYGWIVLRIPASGSLKAKRSVLNKVIKRVQNEFNVSIAQVG
                     QLDDHQFARIGFAMTGNESRFISGKVDHLLRFIEELNVADVIDTKVEMMAVSDFTEAA
                     SWVKGKYDEF"
     gene            4917..5276
                     /gene="rbfA"
                     /locus_tag="PLA58_08860"
     CDS             4917..5276
                     /gene="rbfA"
                     /locus_tag="PLA58_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOR62941.1"
                     /translation="MNFKRADRVGELIMAEMSDILLRSVKDPRLHAVTITAVKVTDDL
                     RNAKIYFVEMGKDELNEEMMAGLTRARGFVRRELGHRLQLRFVPEILFVHDKSFGYGN
                     RIERLLAQIAQQEETHD"
     gene            5269..6219
                     /locus_tag="PLA58_08865"
     CDS             5269..6219
                     /locus_tag="PLA58_08865"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOR62942.1"
                     /translation="MIKDIIAAVASGKRFLITAHVRLDGDALGSELALCRLLSDLGKE
                     AVVCNQDATPGHYRFLPGAERIVHDPVRPELYDVGFVLDCSELERVGEIAADVARIPT
                     LINIDHHVSNGGFCALRLLDPKASSTGELLYRLMREMRAVMTGDICTNLYAAILTDTG
                     GFRYSSTRQETLRAAGDLVKGGADPQWISENIYESDPPAKLQLLARVLETLEMDLDLG
                     IASMTVTEEHLRQTGASMEHTDGFIDIPRTVKGINIAMLYTQTGAKQFKLSLRSKGRV
                     NVEKIARRFGGGGHINAAACRIEGDLETIKSRVLQAVKET"
     gene            6226..7164
                     /gene="truB"
                     /locus_tag="PLA58_08870"
     CDS             6226..7164
                     /gene="truB"
                     /locus_tag="PLA58_08870"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOR62943.1"
                     /translation="MNGIVVIDKPAGLTSHDVVSRVKKILGAKKAGHTGTLDPLATGV
                     LPVCVGEATKLAPFLTADRKRYRATLLLGVETDTQDIQGRETGRSDLVPSEEEIRTAL
                     QSLTGRIQQVPPAFSALKHKGRPLYKYARAGEFPDVAAREVEIFALDVMSIAHPHVTF
                     EISCSKGTYIRTVCADAGRALGCGACLSGLRRLQSGCFTEDMAAPLEEDAAAARPALL
                     EKMLPMAQALPFAAAVEVSEPAAGRLRAGFQPDAEMMRGNVLPFLAAGDMIKFITPEG
                     ELVALAEMQMPSGDIAKLNGKTRIARMLRVFNRLHP"
BASE COUNT         1763 a         1866 c         2269 g         1346 t
ORIGIN      
        1 gaaaaccatc tgttaaatag tgcttgcatt ggctgttcgg gtgtggtaga ggccattcaa
       61 ccatcactga aagttgtttg cagaagtggg cgcccgccca cttttttatt ttcagggaag
      121 acggatatgt acgatgaatt aaaagagaaa atatggcaat tggccgagcc ggtcgtcctt
      181 tcggaaggca tggagctgat tcacgtggaa tgcgtcaaaa tgcattcgcg ctgggttttc
      241 cggtttttcc tggacaagga aggcggcgtc acgctggacg actgcaccaa tgtcagcaac
      301 cagctgggcg atattctgga cgtgcatgat ctgtcccgcg ggccttatac gctggaagtt
      361 tcatcgccgg gcctggatcg tccgatctcg cgcgatcagg attttgagaa attcctgggc
      421 gccaaagtca aaatcagaac tcatggtaaa atagaaggca tcaggaattt tcagggaacg
      481 ctcgccgatt acacggaaga agacggccgg aaggttgttc gaatcgatat ggcggacaag
      541 cgctacagca tcccgaaaga cgaaatcgcc aaagccaatc tggtggatga ggatacagcc
      601 attgcggagt gaaaaaaacg ccgacaagct gaacagaacc gcagccgcga gagaaataag
      661 ggaggattgg atcaatgttg caagacctta aacgtctgat cgagcagatg gggaaggaca
      721 gggggatcga caagcagatt atcacggaag ccctggaggc ggccatgttg acggcggcgc
      781 gcaaaaaact ggggcaggat gtggatattg aagcccatta taacgacgag gccggagaaa
      841 tagaggtttt ccagttcaag acggtcgtgg acaaggtgct tgaccccgaa acgcagattg
      901 ctcatgagga agcccggcga accctggatg aaggcgcgga accgggagac tccctgggga
      961 tgaaaataga aaccagttca ttcggccgca tcgccgtgca gatggccaag cagattatca
     1021 ttcagcgggt caaggatgcg gaacgggaca atatttacga tgaatacaag gaccgcaagg
     1081 gcgaattgat caacgggttt gttcagcggt ttgaaggcgg caatattatc gtcaatctgg
     1141 ggcgcgccga aggcgtggtg ccgcccatgg aacagattca ccgcgagacc tacaagcgcg
     1201 gagagcgcat ccgctcgtat cttttcgagg tcaagaaaaa tacgaagggc gcgcagatca
     1261 tcctgtcgcg cacgcacccc gcttttctga aagccctgtt cgaagtggaa gtgccggaga
     1321 tttccgaagg gctcattgaa atcgtcagcg tggcccgcga gccgggcaag cgcggcaaga
     1381 tcgccgtgag ggccaaggat aaggacattg atccggtggg cgcgtgcgtg ggaatgcgcg
     1441 ggacgcgcgt gcaaagcgtt gttcaggagc tgcgcggcga aaagatcgat atcattccct
     1501 acaccgacga cgcggcaaag ttcgtctcca gcgccctgtc tcccgccaaa gtcaaccggg
     1561 tgctgatcga tgaggaaaac cgcgcgatga cggtgatcgt tcccgacgac cagttgtcgc
     1621 tggccatcgg caaaaacggc cagaatgttc gcctggccgt caagctgacc ggatggaaga
     1681 ttgacgtcaa gagtgaaacc atggccgccg aaaaggacga ggatgcctat gcgtcgttga
     1741 cccgggtgcc cggcatcgga ccggccatgg cggaaaaact ttttgcccag ggcattaaaa
     1801 gcgccgccat gctggcggcg atggagccgg aaacgctttc cgcgattccc ggcgtcggcg
     1861 aaaagaccgc cacggaatgg attgccgaag ccattcggct tttggatgag gaaacaagcg
     1921 atcgtcaaca gggataaatt tttttcaggg ccccaccatt ttatttgagg gagtttcggg
     1981 aatgtcgaaa aagcgagttc acgagttggc caaggaactt ggtctggaaa ataaggatct
     2041 gattgcccac ctggaaaggt taggcattac ggtcaagtcc cacgccagca ccctggaaga
     2101 tcatgaaatc gaaaggatca gggaagacct gcaggccacc tcccccaggc aggttgtgga
     2161 agaaagaatc aaaaccacgg taatccgccg ccgcacggtc cggacgccgg ttgaagaaac
     2221 gccggaggaa aaagaagaaa aaccggaagc cgaaaaggcc gaacgggaag ccgccggagc
     2281 aaaaaaagca aagctcgagg agcagcctgc ggccgcagcc gtcgaggggg aacggaaacc
     2341 ggcgccggcg gaggccgcca aaccgacggt gcccgaaagt ccggttctgc cggagaaaga
     2401 accggctgtg gaagcaaagc ccgagcagcc gacgccggct gcgcccccgg tgaagcccaa
     2461 ggcgccccag gtgattcttc cggtccggcc gccgatgatg tcccgccaca aaatcgttcg
     2521 ggcggaggag aaaaaagaga tcccggccaa ggcgcccgcc aggcccggcg aagcgctcgc
     2581 gcccccggcg ccggagaaaa tcgtcaaaaa agaattcgag aagcccaaga aaaagggcaa
     2641 agggacggtg gaagtcctga tcggcgatga aaaagaagtt ccgcgccgca aaattcttga
     2701 acggaaaatt gataagaaat taaagcgggt cgacgacgac cgggaggttg tgttcagcaa
     2761 atggcgcgaa gacaggaaat ccgcgcccgt caagatgaaa aaaaccgaaa ttacggtgcc
     2821 caaggccatc aaaaggcgcc ttcgcgtggg agacacgatt acaaccggcg aactggccaa
     2881 gagaatgggc gtcaaggccg gcgaggtgat caacaaactg atgggaatgg gcgtcatggc
     2941 caccatcaac cagtcgatcg attttgatat tgcctcgctc gtggcttccg aattcagctt
     3001 ccaggtggaa cgggcggaga tggaatttga agattcgatg ctgaagccgc aggcgtccgg
     3061 agaaaatctc aaaccgcggg ccccgattgt caccatcatg ggccatgtgg atcacggcaa
     3121 aacgtcgctt ttggacgcca tccgtcagac ccatgtgatc gacggcgaag cgggcggcat
     3181 tacgcaggcc atcggcgcct atcacgtcca tatcaatgac cgcgacatcg tctttctgga
     3241 tacccccggt catgaagcct ttacggccat gcgcgcgcgc ggcgcccagg tgacggatat
     3301 cgtcgtgctg gtggtggcgg cggacgacgg agtcatggac cagacggtgg aagccatcaa
     3361 ccactcccgg gtggcgggcg tgcccatcat cgtggccatc aataaaatcg acaaaccggg
     3421 cgccaatccg gatcagatca aacaggcgct ggccgaacac ggtcttttgt ccgaacagtg
     3481 gggcggcgat acgattttca ccgaagtttc ggccaaaaag aaaatcggca tcgaggagct
     3541 tctggagatg attcttctgc aggcggacgt gctggaactc aaagccgatc cggatcaggc
     3601 cgcgcgcggc atcattattg aagccaagct ggaccgcggc cgcggcccgg tggccacggt
     3661 gctgattcag cagggaacgc tccgggaggg cgatgccttt gtttccaaaa cggaatccgg
     3721 ccgcgttcgg gccatgatca acgatcaggg gcgccgcgta aaagaggccg gaccggccat
     3781 gccggtggaa gtcatcggat tttccagcgt tccccagacc ggcgcggaat tcatcagcgt
     3841 ggaggatgaa aagaaagccc ggaacatcgc cgattactgg gttcgcaaga cgcgtgaaaa
     3901 ggagctttcc gcttcctcca agattacgct ggagcagctg tatcagaaga tcaaggaggg
     3961 cgtgaaagac ctcaatgtca ttgtgaaagc ggacgtgcag ggctccatcg aagcaatctc
     4021 cgacgcgttc aagaagcttt cgaccgaaga cgtacagatc aagatgattc acagttcaac
     4081 gggcgcgatc agcgaaacgg atattatgct ggcttccgcc tccaatgcca ttgtgatcgg
     4141 cttcaatgtt cgtccggaca gccgcgtgag cgatctggcg gggcaggaag gcgtggaaat
     4201 caagctctac gatattattt acaatgtgat tgccgacatg aaggcggcga tggagggcct
     4261 tctggagccg gaataccgcg aagtggtcca gggccgcgcc gaagtccgtg aacttttccg
     4321 cgtgcccaag gtgggcatca ttgccggctg ccatgtgacg gacggcaaaa tcacccgcac
     4381 ggccggcatc aaactggttc gcgacagcgt ggcggttttt gacggaaaaa tcctttcctt
     4441 gaagcgattc aaggacgatg cccgggaggt gctggccggc ttcgattgcg gcatcggcat
     4501 tgaaagctat aacgacatcc atgtggggga tgtgatcgaa tcgtatatca cggagaccct
     4561 ggaaagaaaa ttgtagcaaa ggtcggcgat gattatcggc tacggatgga ttgttctgag
     4621 gattccggca tccggttctt taaaggcgaa acggagcgtt ttgaataaag taatcaaacg
     4681 cgtccagaat gaatttaacg tttccattgc ccaggtgggg caactggatg atcatcagtt
     4741 tgcccggatc gggtttgcga tgaccggcaa tgaatcgcgt ttcatcagcg gcaaggtcga
     4801 tcacctgctg cgctttatcg aagagttgaa cgtggcggat gtgatcgaca caaaggtgga
     4861 aatgatggcg gtttccgatt tcacagaggc ggcctcctgg gtaaaaggca agtacgatga
     4921 attttaaaag ggcggatcgc gtcggcgaat tgatcatggc ggaaatgtcc gacattctgc
     4981 ttcggtctgt gaaagatccc cgtctgcatg ccgtgaccat tacggcggtc aaggtgacgg
     5041 atgatctgcg caacgccaag atttactttg tcgaaatggg caaggatgaa ttaaacgaag
     5101 agatgatggc gggattgacc agggccaggg gatttgtcag acgggaactg ggccatcgcc
     5161 tgcagcttcg ttttgtgccg gagattctgt ttgtgcacga taaatccttc ggctacggca
     5221 accgcatcga acggctgctg gcgcaaatcg cccaacagga agaaacccat gattaaggat
     5281 attattgccg ccgttgcctc gggaaagcgg tttctcatca cggcccatgt ccggctggac
     5341 ggggacgctc tgggatcgga gctggccctg tgccgcctgc tttcggatct gggcaaggaa
     5401 gcggtcgtct gcaaccagga cgccacgccc ggtcactacc gttttctgcc gggggcggag
     5461 cgcatcgttc acgatccggt ccgcccggag ctgtatgatg tcggttttgt tctggattgt
     5521 tcggaactgg aacgcgtggg ggaaattgcg gcggacgtgg cccgcattcc aaccctgatc
     5581 aatatcgacc atcatgtttc caacggcggg ttttgcgcgc ttcggctgct ggacccgaag
     5641 gccagctcca cgggggagct gctctaccgg ctgatgcgcg aaatgcgcgc ggtgatgacc
     5701 ggggatatct gcacgaacct ctatgcggcg attttgacgg acacgggcgg tttccgctat
     5761 tccagcaccc gccaggagac gctcagggcg gcgggcgacc tggtgaaagg cggcgccgat
     5821 ccgcagtgga tttccgaaaa catttacgag agcgatcccc cggccaaact gcagcttttg
     5881 gcccgcgttc tggaaacgct ggagatggat ctggatctcg ggattgcgtc catgaccgtg
     5941 acggaagagc atttgcggca aacgggcgcg tcgatggagc acacggacgg tttcatcgat
     6001 attccccgca cggtgaaggg aatcaacatc gcgatgctct acacgcaaac aggcgccaaa
     6061 cagttcaagc tgagcctgcg gtccaagggc cgggtcaacg tggaaaaaat cgcccgccgc
     6121 tttggaggcg gagggcacat caacgcggcg gcctgccgga tcgaaggcga cctggaaacg
     6181 atcaagtccc gggtgctcca agcggttaaa gaaacataag cgtttatgaa cggaatcgtg
     6241 gtgattgaca aaccggccgg gctgacgtcc cacgatgtcg tgagccgggt caaaaagatc
     6301 ctgggcgcga aaaaggccgg gcataccggt acgttggatc ccctggccac cggtgttctg
     6361 ccggtctgcg ttggggaggc gaccaaactg gcgccgtttc tgacggcgga ccgcaaacgg
     6421 taccgggcga ccctgctgct gggcgtcgag acggatacac aggacatcca ggggagggaa
     6481 accggaagat cggaccttgt cccctccgag gaggagatcc ggacggcgct tcagagcctg
     6541 acgggcagga ttcaacaggt tccgccggcc ttttcggcgc tgaagcacaa aggcaggccc
     6601 ctgtacaaat atgcgcgggc cggagaattt ccggacgttg cggcgcgcga ggtggaaatc
     6661 ttcgctctcg acgtaatgag catcgcccat ccgcacgtga cctttgaaat ttcctgctcg
     6721 aaaggcacat acatccggac ggtctgcgcc gatgccggcc gcgcgctggg ctgcggcgcg
     6781 tgtttatccg gattgcgcag gcttcaaagc ggttgtttca ccgaagacat ggccgcgcct
     6841 ctggaagagg atgcggcggc ggcccggccg gcgctgctgg agaaaatgct gccgatggcg
     6901 caggcgctgc cttttgccgc ggccgttgaa gtcagcgaac ctgcggccgg ccggctgcgg
     6961 gccggatttc agccggacgc ggagatgatg cggggcaatg ttcttccttt tcttgcggcg
     7021 ggagatatga taaagttcat cactcccgaa ggcgagctgg tggcgctggc cgaaatgcag
     7081 atgccgtcgg gcgatatcgc gaaattaaac gggaagaccc ggattgccag gatgctcaga
     7141 gtattcaacc gtttgcatcc atgagtgcca aacataaaaa aggacatact tttgataggg
     7201 aggaattgac gtgttaactt tggaaaagag gaaagcatta atcg
//
LOCUS       DAOSRM010000099         6968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_10040_length_6968_cov_102.967887, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000099 DAOSRM010000000
VERSION     DAOSRM010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10040_length_6968_cov_102.967887"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..978)
                     /locus_tag="PLA58_08875"
     CDS             complement(73..978)
                     /locus_tag="PLA58_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62944.1"
                     /translation="MRRIHPRLPFIFCFTFAMCFLLSEGTAFADGIPQVKTDKDVYTE
                     GEKIRVNFFNAPGYSRDWICITASGSADTDAGDYQYMPHGQSQGVLTFEAPVPGKYEV
                     RAYYNYSRNGYVVSARHSFTVAAKDSPAAVVSPPAKPVAQPPVAQPSEKLMESIPTED
                     FSVAGGPANVAIFHFTPLSMDASHYGITVTNVLINSPGMQQAFLVLPKKDLELFLSAN
                     NLQQNDEVDNMVDIGTRLGMNFVIAGTITKRGSTISTSYKIASVAGRGVIHKGQFTSS
                     GEADLIHHIEKMSEDVIAVIRRSVR"
     gene            complement(1134..1469)
                     /locus_tag="PLA58_08880"
     CDS             complement(1134..1469)
                     /locus_tag="PLA58_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOR62945.1"
                     /translation="MYPELFPSAGPPDRRVHGGNGCRHDDRQSHGQSGFFFSTAKDNT
                     VSRIVPHLDADAIVTTPRRDPHYLITEYGAVDLKGKCKRDRALAIIGIAHPDFRDPPM
                     REAEKMRLF"
     gene            complement(1509..2117)
                     /locus_tag="PLA58_08885"
     CDS             complement(1509..2117)
                     /locus_tag="PLA58_08885"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOR62946.1"
                     /translation="MNSPATDITGIILSGGRNTRMGVNKAFLEIGSSRMMDQTLDIFR
                     RLFSEILIVTNDPLSYLEFDDAAVVTDIYKGKGPLGGIYTGLFYAKNPLAFACPCDMP
                     CLNEDFIAYMMKQAGKHDVIVPELPEGYQPLHAIYSKNCLPSIKRLLLMDKLKITGFY
                     RDMRVLTVGEEQIRPFNPDGRLFFNLNTPEDVETLAPERFDL"
     gene            complement(2233..6543)
                     /locus_tag="PLA58_08890"
     CDS             complement(2233..6543)
                     /locus_tag="PLA58_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOR62947.1"
                     /translation="MYSLGINIGSSNVKAALLEGPRVVWSAVEPHEGNFPDTLTKLLS
                     GCDLPPGVQTLTTGTEGRHLLNIHSVIEPLCIEAALQNRKDSIDALVSLGGEDLVVYT
                     IDSQNKIITSFSGNKCASGTGEFFKQQLARMNMTLDDIHRIPEDCRVLKLSSRCSVFM
                     KSDCTHRLNKGEARTGDIVLSLSDVMAVKVADFLKRARIEKGKVLLTGGVTQNPYILR
                     FIRERMPQIEFIVPEQAPWFEAYGAALMAASAGSPLPAPEKLFKQSSVQFKRFKNLKS
                     AEGRVTYLSSQKGKVVAGREYILGVDGGSTTTKASLIDFETSEVTASFYGRTHGDPVR
                     ALKSCLKEIQRQIREDIGDGQIHITLASTTGSSREILGVFLETPAVYNEIIAHSVGTS
                     YYRDGIDTIFEIGGQDAKYVSLKNKVPIDYAMNEACSAGTGSFLEESAQGDLNIPEAA
                     MIGDIALAADAPLKFGEHCSAFINSDIRNAIQQGASREDITAGIVTSIVSNYLNRVVG
                     NRTIGQNIVLQGGVAKNKAVPLAFAMLLDKDILVPPDPELMGCFGVGLLARQKFEEGI
                     LTKQAYDIDAILATDIVYEKEFPCKACDNFCPIRVLVVNGHKYMFGGRCNKYANLRKK
                     KAFDEASVADYIEKRNDILFRECAPDPAALTRKKDVIVGIPRCFSIYTLWPLYSWFFH
                     TMGVETLLSREIPPEGAARVESSYCFPAEIAHGAVQDIYNRQVDYIFLPHFRDMDSYE
                     KTAPANFCPITQSLPYYIRKAFPEIPEEKYLTPVVSFMYGLEKAAEPFVAMAADLGFG
                     EGEAKNAFAVAHEKQMECFAKFAELGRQALEEARRAKRPVIAVLGRPYNSFTTDANMG
                     IPRKYTSRGYSVIPFDILPFADQVIYPNMYWYYGQQDMKAGTLLKNEDNIYITYISNF
                     SCAPDSFMLHYLKWIMGVKPFLILELDSHTADAGVDTRIEAFLDIIEGYRSKIDDIRE
                     ERYDNGLRFINRPGEEIHILNSATGEKIPIKQNPRVKLLLSNMGRLSAELVGATIRGL
                     GINAQTMPVPDVYTLQMARNHASGKECLPSHLVLGSALKYFSSDRYRKDEIYILFVPT
                     TTGPCRTGQYFVFYENLFRDLRLENVVVFTLEADNSYNELGPEFTKYAWWAAVIGDYM
                     KDVETSLRTCAADPAAAMKIYDQIWQKMLAVAEQDITRVLPVLKEIAQTISQIPLARK
                     MEDCAKVLIVGEIYVRRDDFAVDELIGQFSRHGIVGKVSNVAEWFYYCDHVRRYELRK
                     RLRLLPWYRRVFAREFRDIIDWRIEHLYKKRVEKNIRETLGATGLVPRTPHDMQAIMN
                     NTEKHFVSRELHSEIAISSGVAATAMMDGYSGIVNISPFACLIGRVIEGLYTPWARER
                     KYPTISIEIDGNLLPPNVLSKLEIFMLNVKRFRGKGDASAMVERENAQTPVLDRKIIR
                     "
     gene            complement(6751..>6968)
                     /locus_tag="PLA58_08895"
     CDS             complement(6751..>6968)
                     /locus_tag="PLA58_08895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOR62948.1"
                     /translation="ISHLSRPLSLTFRLFGNIAGEDIVLLVVLLLVPLLVPLPIMFLM
                     IFTSVVQTLVFMLLAMMYISGAMEEGH"
BASE COUNT         1441 a         1836 c         2082 g         1609 t
ORIGIN      
        1 gccaaagatg cggagtgttt cattttatcc cttctctgtg taagccggca gccagatggc
       61 gcgtcgggcc ctctagcgca ccgagcgccg gatcactgcg atgacgtctt cgctcatttt
      121 ctcgatatga tggatcagat cggcttcccc ggaagacgtg aactggcctt tgtggatcac
      181 gccgcgcccg gccacgctgg caattttgta gctggtcgaa atcgtcgaac cccttttcgt
      241 gatggttccc gcaatcacaa aattcatccc cagccttgtc ccaatatcga ccatgttgtc
      301 cacctcgtcg ttttgctgga gattattggc ggaaagaaac agttccagat cctttttggg
      361 aaggacgaga aacgcctgct gcataccggg cgagttgatc aggacattgg ttacggtaat
      421 cccgtaatgg gacgcgtcca tgctcaacgg cgtaaaatga aaaatggcca cattggcggg
      481 gccgcctgcc acggagaaat cctctgtcgg aatgctttcc attagttttt ccgacggctg
      541 ggccacgggc ggctgggcca cgggtttggc cggaggtgaa acgactgccg caggggaatc
      601 tttggctgct acggtgaagc tgtggcgggc cgagacgaca tagccgttcc ggctgtagtt
      661 gtaataggcc ctcacttcgt attttcccgg gacgggcgct tcaaaggtca aaacgccctg
      721 gctctgtccg tggggcatgt actgataatc tcctgcgtca gtgtcggcgg aaccggatgc
      781 cgtgatgcag atccagtctc tcgagtatcc gggggcattg aaaaaattca cccggatctt
      841 ttccccttcg gtgtaaacat ccttgtcggt tttcacctgc gggatcccgt cggcaaaggc
      901 cgttccctcc gaaagcaaaa agcacatcgc gaaggtaaaa caaaaaatga atggaagccg
      961 gggatgaatt cgtcgcatcc gctttctcct tttttttgac gccggacaga gcgttttgcc
     1021 catgacgttg gtgtgacgtt tgctgttcgt tcttcagacc gacatcccat ccgccctgca
     1081 gggaatcgcc ccccggccga gaaagtctag cacaacgcga tcaaaaatag aaatcaaaag
     1141 agcctcattt tttccgcttc ccgcatcggc gggtcgcgaa aatccggatg ggcaatgccg
     1201 ataatggcca gagcgcggtc cctcttgcac ttccctttca aatcgacggc gccgtattcc
     1261 gtgatcagat agtggggatc cctgcgcggc gtcgtgacaa tggcgtcggc atccagatgg
     1321 ggaacgatcc tggagacggt gttgtccttt gccgtggaaa agaaaaaccc gctttgtcca
     1381 tgggactgac ggtcgtcatg gcgacatcca tttcctccat gaactcttcg atcaggcggc
     1441 ccggccgatg gaaatagctc aggataaatc gtgaaagtct tctgagaacg cgcccggtcg
     1501 ggaagcggtc agaggtcaaa tctttccggg gcgagcgttt ccacatcctc cggcgtgttg
     1561 agattgaaaa aaagacgtcc gtcggggttg aacggccgga tctgttcttc gccgacggtc
     1621 agcacccgca tatcgcggta aaaaccggtg atcttgagtt tgtccatgag cagcaggcgt
     1681 ttgatggagg gcaggcagtt ttttgaataa atcgcgtgca ggggttgata cccttcggga
     1741 agctcgggaa cgatgacgtc gtgctttccc gcctgtttca tcatgtacgc aataaagtct
     1801 tcattcaagc aaggcatgtc gcacgggcag gcgaaggcca gcgggttttt ggcgtaaaaa
     1861 aggccggtat aaattccgcc cagcggcccc ttgcccttgt aaatgtcggt cacgacggct
     1921 gcgtcgtcaa actccagata ggagaggggg tcgttggtga cgatgaggat ttcggaaaac
     1981 agccggcgaa agatgtcgag cgtctgatcc atcatgcggg acgagccgat ttccagaaag
     2041 gccttgttga cgcccatccg ggtgttcctg ccgccggaaa gaatgatgcc ggtgatgtcg
     2101 gttgcggggc tgttcaatga atccggttcc ttgtcttgag ctttttgtga atggcatatc
     2161 ataaaccgca ccggaaaatt aagtgaaaaa aagcgtcctt cagcgtgaaa gaagtcgcga
     2221 agccggaacg ttttaccgaa tgattttccg gtcaaggacc ggcgtctgcg cgttttcgcg
     2281 ctccaccatg gcagaagcat ctcccttgcc ccggaatctt ttcacgttga gcatgaaaat
     2341 ttccagcttg gacagcacgt tgggcggcag caggttcccg tcgatttcaa tggagatggt
     2401 cggatacttc ctttccctgg cccagggggt gtagagtccc tcgatgacgc ggccgatcag
     2461 gcaggcgaag ggagagatgt tgacgatgcc ggagtagccg tccatcatgg ccgtggccgc
     2521 gacgccgctg gaaatagcga tttccgaatg cagctcccgg ctgacgaaat gcttttccgt
     2581 gttgttcatg atggcctgca tgtcatgcgg tgtgcgcggg accagaccgg ttgcccccag
     2641 cgtttcgcgg atatttttct ccacgcgttt tttgtagagg tgttcgatgc gccagtcgat
     2701 gatgtcccgg aactcccggg caaagacgcg ccggtaccag ggcagcagcc gaagacgctt
     2761 ccggagttcg tagcggcgga catggtcgca gtagtagaac cattcggcga cgttcgacac
     2821 cttgccgaca atgccgtggc ggctgaactg cccgatcagt tcgtccacgg cgaagtcgtc
     2881 tcgacggacg tagatttctc cgacgatcag cactttggcg cagtcctcca tttttctggc
     2941 cagcggtatt tgcgagatgg tttgcgcgat ctccttcaga accggcaaaa cccgcgtaat
     3001 atcctgttcg gccacggcca gcatcttctg ccagatttga tcgtaaatct tcatggctgc
     3061 cgccggatcg gcggcgcacg tcctgaggga cgtttctaca tctttcatgt agtcgccgat
     3121 cacggccgcc caccaggcgt atttggtgaa ttccggtccg agctcgttgt aggaattgtc
     3181 cgcttccagc gtgaagacga cgacgttttc caggcgcagg tcgcgaaaga ggttttcata
     3241 gaacacgaag tactggccgg tccggcaggg acccgtggtc gtcggcacga agagaatgta
     3301 gatttcatcc ttgcggtacc ggtcggaaga aaaatatttc agcgcgctgc ccaacaccag
     3361 gtgcgagggc aggcattcct tgccggaggc gtgattgcgc gccatttgca gcgtgtaaac
     3421 gtccggcacg ggcatggtct gggcgttgat gcccaggccg cggatggtcg cgccgacgag
     3481 ctccgcggag agccggccca tgttggataa aagaagtttg acccgcgggt tttgtttgat
     3541 cgggattttt tcgccggtgg cgctgttgag aatgtggatt tcctcgccgg ggcggttgat
     3601 gaagcgcagg ccgttgtcgt agcgctcctc gcggatgtcg tcgattttgg agcggtagcc
     3661 ttcgatgatg tccagaaacg cctcgatgcg cgtgtccacg ccggcgtcgg ccgtgtggga
     3721 atccagctcc agaatcagaa acggctttac gcccataatc cacttcagat aatgcagcat
     3781 gaaggagtcg ggcgcgcagg agaaattgga aatgtaggta atgtaaatgt tgtcttcgtt
     3841 tttcaggagc gtgccggcct tcatgtcctg ctggccgtag taccagtaca tgttggggta
     3901 gatgacctgg tcggcaaacg gcagaatgtc aaaggggatg accgagtagc cgcgggaagt
     3961 gtatttccgg gggatgccca tgttggcgtc ggtggtaaag gagttgtagg ggcggcccag
     4021 aaccgcgatg acgggtcttt tcgcccggcg cgcctcttcg agcgcctgac gccccagttc
     4081 ggcgaatttg gcaaagcatt ccatttgctt ttcatgggcg acggcaaagg cgtttttcgc
     4141 ttcgccttcg ccgaacccca gatccgccgc catggcgaca aacggttctg cggctttttc
     4201 caacccgtac atgaagctga cgaccggcgt caggtatttt tcttccggaa tctccggaaa
     4261 agctttccgg atgtagtagg gcaggctctg cgtgatgggg cagaaattgg cgggcgccgt
     4321 tttttcatag ctgtccatgt cccgaaaatg cggaagaaaa atatagtcca cctgccggtt
     4381 gtagatgtcc tgcacggcgc cgtgcgcgat ttccgcagga aagcagtagc tgctttccac
     4441 gcgggccgcg ccctccggcg ggatttcccg ggacaggagg gtctctacgc ccatcgtgtg
     4501 gaaaaaccag gaataaagcg gccagagcgt gtaaatggag aagcagcgtg gaatgccgac
     4561 gatcacgtct tttttgcggg taagcgcggc cggatcgggg gcgcattccc gaaatagaat
     4621 gtcgtttcgt ttttcgatgt aatccgccac ggaggcttcg tcgaacgctt ttttctttcg
     4681 aaggttcgcg tatttgttgc agcgtccgcc gaacatgtat ttgtggccgt tgacgacgag
     4741 cacgcggatc gggcagaaat tgtcgcaggc cttgcacgga aattcctttt catagacgat
     4801 gtccgtggcc agaatcgcgt cgatgtcata ggcttgcttg gtcagaatcc cttcttcaaa
     4861 tttctgccgg gccagcaggc cgacgccgaa gcaccccatc agttcggggt cgggcggcac
     4921 caggatatcc ttgtccagga gcattgcaaa cgccaggggc acggccttgt ttttggcgac
     4981 gcctccctgg agaacaatgt tttggccgat cgtccggttg cccaccaccc ggttgaggta
     5041 gttcgacacg atggaggtga caatgcccgc cgtgatgtcc tcgcgggaag cgccctgctg
     5101 aatcgcgttg cggatgtcgg aattgatgaa cgcggagcag tgctcgccga atttcagcgg
     5161 cgcgtcggcc gccagcgcga tgtcgccgat catggccgct tcgggaatgt tcaaatcgcc
     5221 ctgcgccgat tcttccagaa aggacccggt gcccgcggag caggcttcgt tcatcgcgta
     5281 gtcgatcggc accttgtttt tcagcgacac atacttggcg tcctggccgc cgatttcaaa
     5341 gatggtgtcg atgccgtcgc ggtagtagga ggtgccgacg gaatgggcga tgatttcgtt
     5401 gtacacggcg ggcgtttcca gaaaaacgcc gagaatttcg cgcgaggacc cggtcgtgga
     5461 cgcgagcgtg atatgaattt gtccgtcgcc gatgtcttcc cggatttgtc gttgaatctc
     5521 tttgaggcag cttttgaggg cgcgcaccgg gtcgccgtgc gtccgtccgt agaaggatgc
     5581 ggtgacctcg ctggtttcaa agtcgatgag gctggctttc gtggtggtgg agccgccgtc
     5641 cacgccgagg atatattcgc gtccggcgac gacttttcct ttttgcgacg acaggtaggt
     5701 gactctgccc tcagcgcttt tcaggttttt gaaccgtttg aactgaacag agctttgttt
     5761 gaacagcttc tccggggccg gaagcgggct gcctgcggaa gcggccatca gggccgcgcc
     5821 gtatgcctcg aaccagggcg cctgttcggg gacgataaat tcgatttgcg gcatgcgctc
     5881 ccgaatgaag cgcagaatat acgggttttg ggtgacgccg cccgtcagca gaactttccc
     5941 tttttcaatc cgggcccgct tcagaaaatc ggcgaccttg acggccataa cgtcggagag
     6001 ggaaagaaca atatcccccg ttctggcttc gcccttgttc aggcggtgcg tgcagtcgct
     6061 tttcatgaag acggaacagc gcgaggaaag tttcaggaca cggcaatcct ccggaatgcg
     6121 gtggatgtcg tcgagcgtca tgttcattcg ggcgagctgc tgcttgaaga attcgccggt
     6181 gcccgacgcg catttgttgc cggaaaagct ggtaatgatt ttgttttgtg aatcgatcgt
     6241 atagaccacc agatcttcgc cgcccaaaga gacaagcgcg tcgatggaat ccttgcggtt
     6301 ttgcagcgcc gcctcgatac agagcggttc gatgacgctg tgaatgttta aaagatggcg
     6361 tccctcggtg ccggtggtca gggtttgaac gccgggaggc aaatcacagc cggaaagcag
     6421 ctttgtgagc gtgtcgggaa agttgccttc atgaggttcg acggcgctcc acacgacacg
     6481 agggccttcc agcagggcgg ctttgacgtt tgaggagccg atattgattc ccagactgta
     6541 cataaacact cccgtaaaaa tcctgcggat ggaagtatta cgattgtgca aaatcgtcaa
     6601 gcattccagg agattttttt taaatccctt cgaaaattaa ggaattagat ttttgtccgg
     6661 caaaaaaaaa gcagctgaac gcgtctgccg tcagctgctt tttgtttttc gaacattcat
     6721 ttacgggtga atcatccgcc cgtttcagga tcagtgtcct tcctccatcg cgccgctgat
     6781 gtacatcatg gccaggagca tgaaaaccag cgtctggacg accgatgtaa agatcatcaa
     6841 aaacatgatg ggcagcggaa ccagcagggg aaccaggagc agcacgacca gcagaacgat
     6901 gtcttcgccc gcgatattgc cgaagagccg gaacgtcagc gacagaggcc gcgacagatg
     6961 gctgatga
//
LOCUS       DAOSRM010000100         6823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_10320_length_6823_cov_99.318706, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000100 DAOSRM010000000
VERSION     DAOSRM010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10320_length_6823_cov_99.318706"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLA58_08900"
     CDS             <1..249
                     /locus_tag="PLA58_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62949.1"
                     /translation="QIAKQMVDFNKKAFDASFEAMAAMQEQTEKMVSTMMQQSQFFPD
                     EGKKLITDWLKTYKKGREEFKAAADENFKKVDAFFSAK"
     gene            complement(323..754)
                     /locus_tag="PLA58_08905"
     CDS             complement(323..754)
                     /locus_tag="PLA58_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOR62950.1"
                     /translation="MVTGKRIDQMQVGDAAEFAKTVTETDIYLYAGITGDFNPAHVNE
                     AYAKNTFFKTRIAHGMLTAGFVSAIIANQLPGPGTIYMKQELSFLAPVRIGDTITARV
                     EVLELLAEKNRVRLKTTCSNQDGVTVLTGEGLVSPPKASKT"
     gene            complement(775..1830)
                     /locus_tag="PLA58_08910"
     CDS             complement(775..1830)
                     /locus_tag="PLA58_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOR62951.1"
                     /translation="MATEKSYFRALSDLSKEMGTTEDPSRMLHLIVSAAVKTLKVKAA
                     VIFRRDDDTEDAINNAAVAQVGLSKQYSHAGPAHAVKISPQLLKDGYMYFRDATTDRR
                     LANRDKKKKEGIGSILSVPVMDKNRMLGILSVYTKEIRSFTRDEIDFLSILAEQGGSA
                     IEIARLVRRLRNQTQIFLKLAASVSESLDIKDILQALTQDLVQSLNLKAASVRLLGED
                     KRTLKSVASFGLSDKYLRKGPILADKNIAEALKGKTIAIEDVFKDNGIQYKKEKKEEG
                     IVSMLYVPIKALEDVIGVLIVYSGHKRKFTQDEIELVTALAYQGGLAINNACRYMMMQ
                     DDIRDLKENIWSHKSWF"
     gene            complement(1845..3134)
                     /gene="phaC"
                     /locus_tag="PLA58_08915"
     CDS             complement(1845..3134)
                     /gene="phaC"
                     /locus_tag="PLA58_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HOR62952.1"
                     /translation="MAETKIPLDLILERMAEDAEKTRQRVSRASDVLLGELHYEIGST
                     PYEIVYEEDRVRLKHYTRNEEAENKLNVPLLVVYALINRETMLDLQPDRSVVKTFLQE
                     GIDLYMVDWGYPTSKDRFLTIDDHVNGYMDNIVDFIRRKHGVPKINLMGICMGGTFCV
                     IYSALHSEKIKNLITTVTPTNFDTDKGLLHVWMKDVDTDRMVRTFGNIPGDLMNFGFL
                     LLNPARLMIDKYVGFMENMDNRDFVENFVRMERWIFDSPDVPGETFRQFVEDGYKNNL
                     LIQNKMFVGGKKVDLKKITMPLLNFFGEYDHLVPPEACNQLTGAVGSRDAQDVCLETG
                     HIGIYVSSRFQRQFAPRIVQWLKERDREDRVETAPDREMPDSLREAPLKKPPVRKKPA
                     VRKTEATRKAPAGTNISPPARVRKSKQSLIQKIAEAI"
     gene            complement(3194..4105)
                     /locus_tag="PLA58_08920"
     CDS             complement(3194..4105)
                     /locus_tag="PLA58_08920"
                     /inference="COORDINATES: protein motif:HMM:NF021246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HOR62953.1"
                     /translation="MADEGKQFPFDQSSVFPWLKTAADMWLNLAKTLPPNSDTTLKTQ
                     TAVQNRFTQQLETSLNLFKSFSRMMNEPESASAAANTVNALPEIFLKMAGSGFEAAMQ
                     IQNHLMEKAARIGKRTEAYNFDNLDQEVFRALTEVYEKELRQYLKIPPLGLTRFYQER
                     FNEMLDRHNLFETTLAEFLSILYLPMEKSFNVLQEKLKQRTEEGNIPLDVKESYAMWL
                     KILEGHYMNLFKSKEYTDALHRTLNKLEDFLIAKDEAIRDFLQLLPVVTQQDMDEMYK
                     EFHLLKKRVKALEKKAGISPNTLTVVK"
     gene            complement(4200..4700)
                     /locus_tag="PLA58_08925"
     CDS             complement(4200..4700)
                     /locus_tag="PLA58_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR62954.1"
                     /translation="MNNTPVFIHGLESTSQGNKAQYFRKMFPEMIIGDYTGGFAARMQ
                     KLRSVLAGKTNLILVGSSYGGLMAARFALDNEALIKKLILLAPALTIEGFEQAVRRKL
                     QIPTVLYHGTRDEVVPPQAVKAIAEKTFARLEHHFVEDDHPLNAVFPTLPWPVLLEQR
                     PAAEID"
     gene            4803..6029
                     /locus_tag="PLA58_08930"
     CDS             4803..6029
                     /locus_tag="PLA58_08930"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOR62955.1"
                     /translation="MALVLPFKAVRPQVKYAPQVAALPYDVMTREEGRMAVAGRPLSF
                     MHVEKSEIDVPDGTRPDNPVIYETAKRNFARMREEGILIQDPSPCYYIYRQKMGSHVQ
                     TGIVGVMSAAEYDEGKIKKHELTRQDKEDDRIRHVDQVNAQTGPVFISYRTRPGLNEI
                     VAKITAGRPEYEFTAEDGVVHTAWVVADQARMDAIRSEFDQVDALYIADGHHRAAAGA
                     TIARKRRKPSQAQESDGVLAVFFPHDQLKVMDYNRAVKDLRGLSAEQFLEKISSSFIV
                     TKGFAAKSPQRLHDFGMYLGGAWYCLTIKEGAYDAKDPVASLDAAILQDHLLAPVLGI
                     ADPRTDDRIKFIGGIRGMAELEKLVDQDGFAVAFSLHPTTMEQIMNVADAGAIMPPKS
                     TWFEPKLRSGLFVHCL"
     gene            complement(6222..>6823)
                     /locus_tag="PLA58_08935"
     CDS             complement(6222..>6823)
                     /locus_tag="PLA58_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62956.1"
                     /translation="VVHPSLSFHALRSLPPAGLGQRDVLTHKPGLAPFALQRFGGRHG
                     RIQEHAAELPADIDIALLSLAYHHMPWDDKATHLREMSGKVNHLLLMEMDGDNDTPQL
                     GSPELAVSVYQSYGPLIDAIFAHDAPITVTQSCVDNFVMAEVISFLTQPRGRRNDYHM
                     LRGQWTRIFSENLDGHHPRGEATVLTSDGCEFFACHWSR"
BASE COUNT         1341 a         1859 c         1975 g         1648 t
ORIGIN      
        1 cagatcgcaa aacagatggt ggattttaac aagaaggctt ttgacgcctc gtttgaggca
       61 atggccgcca tgcaggaaca gacggagaag atggtctcca cgatgatgca gcagtcgcag
      121 ttttttccgg atgaaggcaa gaagctgatc acggactggc tgaaaaccta caagaagggc
      181 cgtgaggaat tcaaggcggc ggccgacgaa aattttaaaa aggtcgacgc gtttttttca
      241 gccaagtaac gaatgtctca aaatgaaaaa gccgccgcag ggagtcgcgg cggttttttc
      301 atttaaaatc ggcaaaacgg cgtcaggttt ttgacgcctt cggcggactg accagtcctt
      361 cacctgtgag cacggtcacg ccgtcctgat tgctgcacgt cgttttcagg cggacccggt
      421 tcttttcggc gagaagctcc agaacttcaa cgcgcgccgt aatggtgtcg ccgatgcgca
      481 ccggcgcgag aaaactcaac tcctgcttca tgtaaatggt cccgggtccc ggcagttgat
      541 tggcgataat ggcggagacg aagccggccg tgagcatgcc gtgcgcgatg cgggttttga
      601 aaaacgtgtt tttggcgtac gcctcgttga cgtgcgccgg attgaaatcg ccggtaatcc
      661 ccgcatacag gtaaatatcg gtttccgtca cggtcttggc gaattccgcc gcgtccccca
      721 cctgcatctg atcaatcctt tttcctgtga ccatcccttt tccccctctt tgcgttaaaa
      781 ccaggactta tggctccata tattctcctt caggtccctg atgtcatcct gcatcatcat
      841 gtaacggcag gcattgttga tggccaggcc tccctgatac gccagggcgg tcacgagctc
      901 aatctcgtcc tgggtgaatt ttcttttgtg cccgctgtat acgataagaa cgccgatgac
      961 gtcttccagg gctttaatcg gcacgtacag catggagacg atgccttcct ccttcttttc
     1021 cttcttgtac tgaatgccgt tgtccttgaa aacgtcctcg atcgcgatgg tttttccctt
     1081 gagcgcctcg gcgatgtttt tgtccgccag aatcgggcct ttccgaaggt atttatcgct
     1141 gaggccgaag ctggccaccg atttcagcgt gcgcttgtct tcgcccagca gccgcacgga
     1201 cgccgccttg agattgagag attgcaccag atcctgcgtc agggcttgca gaatgtcttt
     1261 gatgtcgagg ctttctgaga cgctggcggc cagtttcagg aaaatctggg tctggttgcg
     1321 cagcctgcgg acgaggcggg cgatttcaat cgcggatccc ccctgctcgg ccagaataga
     1381 cagaaaatcg atttcgtccc tggtgaacga gcggatttct ttggtgtaaa cgctcaggat
     1441 gcccagcatc cggtttttgt ccatgaccgg aacggacaga atggagccga tcccctcttt
     1501 tttcttcttg tcgcggttgg ccagccgccg gtccgtcgtg gcgtcccgga aatacatgta
     1561 gccgtctttc agcagttgcg ggctgatttt gacggcgtgc gccggcccgg catgcgagta
     1621 ttgtttggac agcccgacct gggccacggc cgcattgttg atggcgtcct ccgtgtcgtc
     1681 atctctccgg aaaatcacgg ctgccttaac tttcagcgtt ttgaccgccg cggacacaat
     1741 cagatggagc attctcgacg gatcttcggt ggtgcccatt tccttgctga gatcgctcaa
     1801 cgctctgaaa taactttttt ccgttgccat actttttctt cctttcagat cgcttccgca
     1861 attttttgga tgagggactg cttgcttttc cggacccgcg ccggggggct gatgttcgtc
     1921 ccggcgggcg cctttcgcgt tgcttcggtt ttccggacgg ccggtttttt ccggaccggc
     1981 ggctttttca acggcgcttc acgcagactg tcgggcattt cgcgatccgg ggccgtctct
     2041 accctgtcct cccggtcccg ctccttgagc cattgcacga tgcggggcgc gaactgccgc
     2101 tggaacctgg agctgacata aatgccgata tgcccggttt ccaggcagac gtcctgcgca
     2161 tccctgctcc ccacggctcc ggtcagttga ttgcaggctt ccgggggaac gagatggtca
     2221 tactcgccga aaaagttcaa aagcggcatg gtgatttttt tcaaatccac tttttttccg
     2281 cccacaaaca ttttgttttg aatcagcaga ttgtttttgt agccgtcttc cacaaactgg
     2341 cggaaggttt ccccggggac gtcggggctg tcgaagatcc agcgctccat gcgcacgaag
     2401 ttttccacga aatcccgatt gtccatgttt tccatgaagc cgacgtactt gtcgatcatc
     2461 agacgggcgg ggttgagcag caggaagccg aaattcatca gatcgccggg aatattgccg
     2521 aaggtgcgga ccatccgatc cgtgtccacg tccttcatcc agacgtgcag caggcctttg
     2581 tccgtgtcga agttggtcgg cgtgacggtg gtgatcaggt ttttgatttt ttccgaatgc
     2641 agcgcggagt aaatgacgca aaaggttccg cccatgcaga tgcccatcag attgattttg
     2701 gggacgccgt gtttccgccg gatgaaatcc acaatattgt ccatgtagcc gttgacatga
     2761 tcgtcaatgg tcaggaaacg atccttgctg gtgggatagc cccagtccac catgtacagg
     2821 tcgatgcctt cctgcaaaaa cgttttcacg acgcttcggt ccggctgcag gtccagcatc
     2881 gtttcgcggt tgatcagcgc gtacaccacc agcagcggga cgttcagctt gttttccgcc
     2941 tcttcgttgc gggtgtagtg cttgaggcgg acgcggtctt cttcgtacac gatttcataa
     3001 ggcgtcgatc cgatttcgta atggagttca cccagcagga cgtcggaggc gcgggaaacc
     3061 cgctgccggg tcttttcagc gtcttcggcc attcgttcca gaatcagatc caggggaatt
     3121 tttgtttcgg ccatgagaac gccatccttc cgaaaacttt tcttcccgtc cggggcaggg
     3181 gcggaaagag cctttatttg acaaccgtca gggtgttggg ggatatgccg gcttttttct
     3241 ccagcgcttt gaccctcttt ttgagcaggt gaaactcctt gtacatctcg tccatatctt
     3301 gttgcgtgac cacgggcagc agttggagaa agtcgcgaat ggcttcatcc ttggcgatga
     3361 gaaaatcctc cagcttgttg agcgtacggt gcagcgcgtc cgtgtattct ttggacttga
     3421 acaggttcat gtagtggcct tccaggattt tcagccacat ggcgtagctt tccttcacgt
     3481 cgagggggat gtttccctct tccgtccgct gcttgagctt ttcctgaagc acgttgaagg
     3541 acttttccat cggaagatac aggatggaga gaaattccgc cagcgtggtt tcaaagaggt
     3601 tatgcctgtc cagcatctcg ttgaagcgct cctggtaaaa ccgcgtcagt cccagcggag
     3661 gaatcttcag gtattggcga agctcttttt catagacttc ggtgagcgcc cggaagactt
     3721 cctgatccag attgtcgaag ttgtacgctt ccgttcgctt gccgattcgg gcggcttttt
     3781 ccatcagatg gttctggatc tgcatggccg cctcaaagcc ggatccggcc atcttcagaa
     3841 aaatctcggg cagcgcgttc accgtattgg cggcggccga tgccgattcc ggctcgttca
     3901 tcatgcggga aaaggatttg aacagattca ggctggtttc gagctgctgc gtgaaacggt
     3961 tttgcacggc ggtttgcgtt ttcagcgtcg tgtccgaatt gggcggaagg gtttttgcca
     4021 ggttgagcca catatcggcg gcggttttca gccacgggaa aaccgatgat tgatcgaatg
     4081 ggaattgctt gccttcgtca gccatgatga cctctttttt tgaagccgcg atccggaaaa
     4141 catcgttgag atcacttaca gtaaacgcgg ttgattttta atccatccgc cggtgtttgt
     4201 caatcgattt cggccgccgg cctctgttcc agcagcacgg gccatggaag cgtcggaaaa
     4261 acggcgttga gcggatggtc gtcctccacg aagtgatgct ccagtcgggc gaaggttttt
     4321 tcggcgatgg ctttgacggc ctgcgggggg acgacctcgt cgcgtgttcc gtgatacagg
     4381 accgtcggaa tttgaagttt ccgccggacg gcttgctcaa atccttcgat ggtcagggcg
     4441 ggggccagca ggatcagctt tttgatcagc gcttcgttgt ccagcgcgaa gcgggccgcc
     4501 atcaggccac cgtagctgga tcccaccaga atcagattgg ttttgcctgc aaggacgctt
     4561 cgaagcttct gcattcgggc cgcgaaaccg cccgtgtaat ccccgatgat catttccgga
     4621 aacatttttc gaaagtactg ggccttgttc ccctgggaag tgctttccag accgtgaata
     4681 aataccggcg tgttgttcat agacgtcctt tcctgcggag aatcgaattt tattttgacg
     4741 ttgcccggat tatgccattg tgttaaatcg tgcgcaactt tattttgaaa aaggagaagg
     4801 gtatggctct ggtattgcct tttaaagcgg ttcgaccgca ggtgaaatat gcgccgcagg
     4861 tggcggcgct tccttatgac gtcatgacga gggaagaagg tcggatggcg gttgcgggcc
     4921 gtccgctcag ctttatgcac gtggaaaaat cggagattga cgttccggac ggcacgcgtc
     4981 cggataatcc cgtgatctat gagacggcga aacgcaactt tgcccggatg cgggaagagg
     5041 ggattctgat tcaggacccg tccccctgct attacatcta tcgccagaag atggggtccc
     5101 atgtgcagac gggcattgtc ggcgtcatga gcgccgcgga atacgacgag ggaaaaatca
     5161 agaagcacga actgacccgg caggacaagg aggacgaccg catccgccac gtcgatcagg
     5221 tcaacgcgca gaccggtccc gtattcatca gctaccgcac gcgccccggc ttgaacgaaa
     5281 tcgtggcaaa gatcaccgcc ggccggccgg aatatgagtt caccgccgaa gacggcgtgg
     5341 tccacaccgc gtgggtggtg gcggatcagg cccggatgga cgccatccgg tcggagttcg
     5401 atcaggtgga cgccctgtac attgccgacg gacatcaccg cgccgcggcg ggcgccacca
     5461 tcgcccgcaa acgccgcaag ccctcgcagg cgcaggagtc ggacggcgtg ctggcggtgt
     5521 tttttcccca cgatcagctc aaagtcatgg attacaaccg ggccgtgaag gacctccgcg
     5581 gcctgtcggc cgaacagttt ctggaaaaaa tttcctcatc gtttatcgtg acgaaaggtt
     5641 ttgccgcaaa atcgccgcag cgtctgcatg atttcgggat gtatctgggc ggcgcatggt
     5701 attgcctcac gattaaagaa ggcgcgtacg acgcgaaaga tccggtggcc agcctcgatg
     5761 cggccatttt gcaggatcat ctgctcgcgc cggttttggg tattgccgat ccgcgaaccg
     5821 acgaccggat caaatttatc ggcggtatcc gggggatggc ggaactcgaa aaactggtgg
     5881 atcaggacgg atttgccgtg gccttttcac ttcatcccac aacgatggag cagatcatga
     5941 acgtggcgga tgcgggcgcg atcatgcccc ccaagtccac ctggtttgag cccaagctgc
     6001 gcagcggctt gtttgtccat tgtctgtagg gcgcgttttc caatcgcgcc ggttttcttg
     6061 cgttttcccc aaacgcgcgg gcatcgtgca ttctgcggcg caatcgggga ttgcgccctg
     6121 caggcttttg ccgtttgccg tatggagttg ggccgcgtat cggcgggcat gggcggcgaa
     6181 tgcgaaaagc aggcaatccc ggctgccccg ggccggaagt gctacctgct ccagtggcag
     6241 gcaaagaact cgcatccgtc cgacgtcagc accgttgctt ctccccgggg atgatgtcca
     6301 tcgagattct cgctgaaaat ccgcgtccac tggccgcgca gcatgtggta gtcattgcgc
     6361 cggccgcggg gctgcgtcag aaacgaaatc acctccgcca tcacaaaatt gtccacgcag
     6421 ctctgtgtca cggtgatggg ggcgtcatgg gcaaagatgg cgtcgatcag cgggccgtag
     6481 gactggtaaa ccgacacggc cagctccgga gaaccaagct gtggggtgtc gttgtctccg
     6541 tccatttcca tcagcagcag atgattcact ttgccgctca tttcacgaag gtgcgtcgct
     6601 ttatcgtccc agggcatgtg gtggtaggcc agggacagga gcgcgatatc aatgtctgcg
     6661 ggcagttctg cggcatgctc ctggatccgg ccgtggcgcc ctccgaaacg ctgaagcgca
     6721 aacggggcaa gccccggctt gtgcgtcagg acgtctcttt gcccaagccc cgctgggggg
     6781 agggaacgca aggcatgaaa cgaaagagag ggatggacaa cca
//
LOCUS       DAOSRM010000101         6775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_10401_length_6775_cov_77.933482, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000101 DAOSRM010000000
VERSION     DAOSRM010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10401_length_6775_cov_77.933482"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1767)
                     /locus_tag="PLA58_08940"
     CDS             complement(163..1767)
                     /locus_tag="PLA58_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR62957.1"
                     /translation="MDYFNILHFKKEPFSNSPEPEFLFAAPQYDICLQMLEMSVRLRR
                     GLSVVIGDVGTGKTTLCRKLIQNLSAPTPSESPEIDTYLLLDPAVESSIVFVKTVAGI
                     LGIRDITPEDSEWQLKEKIKNFLFEKGVDEKRIIVLIIDEGQKIPDECLEILREFLNY
                     ETNDAKLLQTIIFAQPELEKSLAARPNLMDRVDYIHHFKPLNFWQMKAMIEYRVSVAR
                     QETVKYPLFTLSGLLAIYFATGGYPRKVVSLCHQALLKMIIRGKRQAGWFLVRSCSSR
                     TKAQAQTGRRVVWRAASLLVMATVLFLTALYLIGPENPQNGASMSQALTGIFHKKESS
                     LPSAPILPAPSVPAVPPTAAVSPLPEQPGKNIAPEKTPEPLLGSIPAKGKFTLWKIMD
                     NIYGDAGEEIMRQFILANPQIKDSRNIAENAVIQVPLIMDKARPIPPGTILVSLQSGK
                     DLAPIYYSFIERKDRSGAPGRMFLSFWSKREGRKFAVALNKRFESLQAAQDAVNRLPL
                     EVAASAKILSAWDGDTVCFNRKLAGI"
     gene            complement(1783..3429)
                     /gene="pilQ"
                     /locus_tag="PLA58_08945"
     CDS             complement(1783..3429)
                     /gene="pilQ"
                     /locus_tag="PLA58_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HOR62958.1"
                     /translation="MTLRKRHTQAYLVATGILLLSLLVGCAGDLKSKKPDPFFEKWET
                     MAEASRGSSPADRQKTITLPTPSDQKAQLSALIGDKSQKLPTKPINLTMRQADLKAVL
                     RAMAKAVNTNILIKNDLKGDVSVDFRSVPWDQAFTGLLKTHGLSYVWEGSIIRVMSIE
                     DIDHELKQKVQMREIDWVEPLMDPVIIKIDYADPKKLEKTLQNFLTKDKEGKTRGSVT
                     LDEHSNSMIISASARDLERMVPIIEKLDKSTPQILIKANIVETSKSVARDLGIKWGGY
                     NSGNLSGNQLVVTGGRGVPSGGRTYTTDPTDKQISLGSGTMGVDFPAGSAGSQLLDAA
                     TGPLGTLGLMFGVVGGNLLEMELQALQSDSKINIVSSPSITTLDNQKAFTENGKKVPF
                     VTLQPSTTPGVLPTQTVTFQDAVMRLEITPHVIDGKNLKMQIKVSKDELDFSRAADMY
                     GNPIINKKLTETSLIVQDGETIVISGLTKETNTAGDAGWPWLKDIPVLGWAFKADSRS
                     EDMEEVLIFITPHILKVAGINERLSSGIQEKPASGQKGIN"
     gene            complement(3651..4145)
                     /locus_tag="PLA58_08950"
     CDS             complement(3651..4145)
                     /locus_tag="PLA58_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62959.1"
                     /translation="MKKLNTRQMIILAVTAVAVLYAGYELLIAGPSAKKAGKATAPVE
                     EKAALAALTSDIMSNKATAADVYVAQRAEAEWSKNPFWDKASYRAFAQKEEMKGVKEG
                     PKIVYSGYVETGAKKMAIINGWEYEAGQSLDVEGYLLKRVTPSRVLIINRTTGGETYV
                     PIQE"
     gene            complement(4161..4700)
                     /locus_tag="PLA58_08955"
     CDS             complement(4161..4700)
                     /locus_tag="PLA58_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62960.1"
                     /translation="MIKKIMEKIPSKNVGYMIICGGIILLIILLGILPLHRYNVKRSE
                     AVAGIQAQIDEQKELRQIYQLISQASLKKDDRILPNPMKTKLSRQNMDEFQDSFRAEA
                     AKAGINILSLIPDVKTMAGGSQNLLYTAMLKGEFADFRKLMIGLGSLPYIDRIEGINM
                     EQNSDSMDFELKIWVALAG"
     gene            complement(4703..6373)
                     /gene="pilM"
                     /locus_tag="PLA58_08960"
     CDS             complement(4703..6373)
                     /gene="pilM"
                     /locus_tag="PLA58_08960"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOR62961.1"
                     /translation="MDIGANAVTMVKMRKTSDGKSLLVDQKVVKLGEGISKKSPEFNT
                     LLKSSLTAFAGSLDDCDIWAMMSAAEVNVFRLKIPIVPANQMENVIYWAAKKENPIDE
                     KEAFFDYEVQGKINDQGIPKYAVMVYSAPRSEPQKVKDLFSSIGVSLTGVTIAPFAIQ
                     NYFRTEWIKAGEGAFAGIFIGHDFSRIDIYRKNDLVMTRVIKTGISSMMEAIDDAIAE
                     ASPGAREEKERAKALLNEIAADPEKWMKEDGGVNWAETGILEMTTPALERLLRQIERT
                     MEYFATSVGFEKVEKVYLSAAISVFYYPLLHYLREQIGSISEYFDPFAGKPFPVKSGL
                     SSLAERASLIPAIGLALSDSKHTPNAIFTYAQKKQELIRKRINRGVFSTFAAALAVCL
                     VIMALQVSEARQLGVQKGKLEKELLLLGPKLSKDKIAEMAKEMKIRNQHNQRYSQKYK
                     GLALISELSFLTPDDIRLTQVRMSIPEPGAGGQTTGAAGKEAAPKENLVLQGVVLGSR
                     GAMDAQLAQYVMKLENSPMLQGVVLQKSSVAKFRNSEILQFVINAKIG"
     gene            6447..6641
                     /locus_tag="PLA58_08965"
     CDS             6447..6641
                     /locus_tag="PLA58_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62962.1"
                     /translation="MKEIFGPLPGVAGKGGFAPRITFNNFSVELISFAFAKELTQKTT
                     FFAPNRHPNISNKCCFYPKP"
BASE COUNT         1495 a         1817 c         1641 g         1822 t
ORIGIN      
        1 tttgtcattt cgaccctctt ctttgtcatt tcgaccgaag ggagaaatcc ttgtcccgaa
       61 tacaatgaga gatcgttaga tttctcgtcg cttcgctcct cgaaatgaca tcaggcggtg
      121 cattttggtc ctcgatatga caaaattcaa aagtttcact cttcagatgc cggcaagctt
      181 gcggttaaag caaaccgtgt cgccgtccca tgccgacagt atttttgcgc ttgccgcaac
      241 ttccagcggc agtcgattga cggcatcctg tgccgcctgt aagctctcaa agcgcttgtt
      301 cagcgccacc gcgaactttc tgccttcgcg cttgctccag aaagaaagaa acatccgtcc
      361 gggcgcgccg gaccggtctt ttctttcgat aaacgaatag taaataggcg ccagatcttt
      421 gccgctttgc agggaaacca gaatcgtacc cggcggaatc ggccgcgctt tgtccatgat
      481 cagcggcacc tgaatgacgg cgttttccgc aatattgcgg ctgtctttga tttgcgggtt
      541 ggccagaata aattgccgca tgatttcttc gcctgcatct ccataaatat tatccatgat
      601 cttccacaga gtaaacttgc cctttgcggg gatggagcct aaaagcggct ctggtgtttt
      661 ttccggagcg atgttttttc ccggttgttc cggcaacgga gaaacggcgg ctgtcggcgg
      721 aacggcggga acagaaggcg ccgggagtat cggcgcggaa gggagtgaag attctttttt
      781 atggaatatg ccggtcagcg cctgactcat ggatgcgccg ttttgtgggt tctccggtcc
      841 gatcagatac agcgcagtta aaaaaagcac ggtggccatc accaaaagac ttgccgccct
      901 ccataccacc cgcctgccgg tctgcgcctg cgcttttgtc ctgctcgaac agcttcgaac
      961 cagaaaccag cctgcttgtc ttttgccccg aatgatcatc ttgagcagcg cctggtggca
     1021 cagcgaaaca accttacggg gataaccgcc ggtggcgaaa taaatggcca gcagtccgct
     1081 gagagtaaag aggggatatt ttaccgtttc ctgacgggcg acggagaccc ggtattcaat
     1141 catcgctttc atctgccaga agttcaacgg cttgaaatga tgaatataat caacacggtc
     1201 catcaagttg ggccgggcgg ccagactctt ctccagctcc ggctgcgcga aaatgatcgt
     1261 ctggagcagt ttggcatcgt tggtttcata gtttaaaaat tcccgcaata tttccaggca
     1321 ctcgtcaggg atcttttgcc cctcgtcaat aatcaggaca atgattcttt tttcatccac
     1381 gcctttttca aacaggaagt tcttgatttt ttccttgagc tgccattcgc tgtcttctgg
     1441 ggttatgtcg cgaatcccca ggatcccggc aacggttttg acaaagacga ttgagctttc
     1501 caccgcgggg tccagcaaaa gataggtatc gatctccggc gactccgaag gtgtgggtgc
     1561 agataaattc tggatcagct tgcggcagag ggttgttttc ccggtgccca catcgccgat
     1621 caccacgctg agtccgcgcc tcagacgaac ggacatctcg agcatctgaa gacagatgtc
     1681 gtactgcggc gccgcgaaaa gaaactccgg ttccggagaa ttggaaaaag gttccttttt
     1741 gaaatgaaga atattgaaat aatccatttc cggcgcctcc ctttaattaa tgcccttctg
     1801 cccggaagcc ggtttttcct gaatccccga ggaaagccgc tcattgatgc cggcgacctt
     1861 caggatatgc ggcgtaataa aaatcagaac ctcttccatg tcttcggacc ggctgtcggc
     1921 tttaaaggcc catcccaaaa ccggaatgtc tttcagccac ggccagcccg catcgccggc
     1981 cgtattggtt tctttggtca accctgaaat cacgatggtt tccccgtctt ggacaataag
     2041 cgaggtttcg gtgagcttct tgttgatgat gggattgccg tacatatcgg cggcgcggct
     2101 gaaatccagt tcatctttcg aaaccttgat ctgcatcttc aggtttttgc cgtcaatcac
     2161 atggggcgta atttccagcc tcatcacggc gtcctgaaaa gtcacggttt gtgtggggag
     2221 aacgccaggc gttgtgctgg gctgcaatgt aacaaaagga actttctttc cgttctcggt
     2281 aaaggctttc tggttgtcca gcgttgtgat cgagggactc gaaacaatat tgatcttact
     2341 gtcgctctgc agagcctgca attccatttc cagtaaattt ccaccgacga cgccgaacat
     2401 caggcccaac gtgccgaggg gaccggttgc ggcatccagt aattgcgatc ccgcgctgcc
     2461 ggccgggaaa tcaaccccca ttgttcccga ccccagggat atttgcttgt cggtaggatc
     2521 ggtagtgtac gttcttccgc cggatggcac ccctctgccg ccggtaacca caagctggtt
     2581 gccgctcaaa ttaccgctgt tgtatccgcc ccacttgatc cccagatcgc gcgcgacgct
     2641 ttttgacgtt tcgacaatat tggccttgat caggatctgc ggcgtcgatt tgtccagctt
     2701 ttcaataatg ggaaccatgc gctccagatc gcgcgcgctt gcggagatga tcatggaatt
     2761 gctgtgttca tcgagcgtta cagagcctct tgtcttgcct tctttgtctt tcgttagaaa
     2821 gttctgcagc gtcttctcca gttttttcgg atcggcatag tcgattttga tgattacagg
     2881 gtccatcagg ggttccaccc agtcaatttc acgcatctgc actttctgct tcaattcatg
     2941 gtcaatgtct tcaatggaca tgacgcggat gatgctgcct tcccacacat aactcaaccc
     3001 gtgcgtcttt aacagaccgg taaaggcctg atcccagggg accgagcgga aatcgacgct
     3061 gacgtcgcct ttcaaatcat ttttaattaa gatattggta ttgacggctt tggccatggc
     3121 ccgcagcacg gccttgagat ccgcctgccg catggtcaga ttgatcggct tggtcggaag
     3181 tttttgggat ttatccccta tcagagcgga aagttgcgct ttttggtcgg aaggcgtcgg
     3241 aagtgttatg gtcttttgcc tgtctgccgg agaagatccg cgggaagcct ccgccatcgt
     3301 ttcccatttt tcaaaaaacg ggtccggctt tttgcttttg aggtcccctg cgcatccgac
     3361 gaggagcgaa agcaaaagaa tgcctgttgc aactaaatat gcttgtgtgt gtctttttcg
     3421 taaggtcaag gcctcaacct cctgtttttt cgcttttcca aaaacccgcg gggctttcta
     3481 ttcgagagct tttcacaacc cctctagccg tcataccggc gaaggccggt atccagacgt
     3541 cgtccccgcg gaggcgggga accagttcct ttaatactgg attcccgccc cccgacttct
     3601 cgagggcagg cttcgcggga acgacaggaa accagtttgt gcaaaggttt ctattcctga
     3661 atcggcacat aggtttcgcc gcccgtggtg cgattgataa tcagaacgcg cgatggcgtc
     3721 actcttttca aaaggtatcc ttcgacgtca agggactgac cggcttcgta ttcccaaccg
     3781 ttgataatcg ccattttctt agccccggtt tcgacataac cggaataaac gattttgggc
     3841 ccctctttga cgcctttcat ttcctccttc tgggcaaagg cgcggtagga agccttgtcc
     3901 caaaaaggat ttttgctcca ctcggcctcc gctctttgag ccacataaac atcggctgcg
     3961 gttgccttgt tcgacatgat atcactggtc aatgcggcaa gggcagcttt ttcttcaaca
     4021 ggcgcggttg ctttgccggc ctttttggcg gaaggaccgg caatcagcaa ttcatagccg
     4081 gcatacagga ccgcaacagc ggtaaccgcc agaatgatca tttgtctagt gttgagcttc
     4141 ttcattcttt ccgcatccgt ctatccggcc agggccaccc atatcttcaa ttcaaaatcc
     4201 atggagtcgc tgttttgctc catgttgatc ccttcgatcc ggtcgatgta cggcagcgac
     4261 cccagcccga tcatcaattt ccggaaatcg gcaaactctc ctttcagcat ggctgtgtaa
     4321 agaaggtttt gcgaaccgcc ggccatggtt tttacatccg gaatgaggga gagaatgttg
     4381 atgccggcct tggccgcttc cgcccggaaa gaatcctgaa attcatccat gttctgccgg
     4441 gacagtttcg ttttcatcgg gttcggcaga attcggtcgt cttttttcaa cgaagcctgg
     4501 ctgatcaact gataaatctg ccgcagttcc ttctgctcgt cgatttgcgc ctgaatgccc
     4561 gcgacagcct ccgaacgctt gacgttgtat cgatgcaggg gaagaatccc cagcaaaatg
     4621 atcagaagaa taatgccacc gcagatgatc atgtatccca cgtttttgga cggaattttc
     4681 tccattattt tctttatcat cgctacccta ttttcgcgtt gatcacaaac tgcagaattt
     4741 cactgttcct gaattttgcg acgctgcttt tctgcagaac cacaccctgc agcatgggtg
     4801 aattttccag tttcatgaca tattgcgcca gctgcgcgtc catcgcgccg cgggaaccca
     4861 ggacgacgcc ctggagaaca agattttctt ttggcgccgc ttcctttccg gctgccccgg
     4921 ttgtttgccc tccggcgccc ggctccggaa tggacatccg cacctgggtc aggcgaatat
     4981 catccggcgt caggaaagac aattcgctga tcagcgccag acctttgtat ttctgtgaat
     5041 accgctggtt gtgctgattg cgaattttca tttctttggc catttcagca attttatctt
     5101 tagacaactt cgggcccaag agcaagagtt ctttttcgag ttttcctttt tgaacaccaa
     5161 gctgccttgc ctcgctgacc tgaagagcca taatgacgag gcagacggcc aaagcggccg
     5221 cgaacgtcga aaaaacgccg cggttgatcc tcttgcggat cagctcctgt tttttctgcg
     5281 cgtaggtgaa gatggcattc ggcgtgtgtt tgctgtccga cagggccagc ccgatggccg
     5341 gaatcagcga ggctctttcc gccagagacg aaagcccgct tttgaccgga aatggtttgc
     5401 ctgcaaacgg atcaaaatat tcactgatgg aaccgatttg ttctctgagg taatgcagca
     5461 gggggtaata aaatacgctg atcgcagccg aaagataaac tttctccacc ttctcaaaac
     5521 cgacggaggt ggcgaagtat tccatcgttc tttcgatctg acgcagcagg cgctccagag
     5581 cgggagtcgt catctccaga attccggttt cggcccagtt gacgccgcca tcctctttca
     5641 tccatttttc cggatcggcg gcaatttcat tcaataaggc tttggctctc tccttttctt
     5701 ccctggcgcc cggggacgct tccgcgatgg catcatcgat agcctccatc atgctgctga
     5761 tgcctgtctt gattacccgt gtcatcacca ggtcattttt ccgatagatg tcgattctcg
     5821 aaaaatcatg tccgataaaa atgccggcaa aggccccttc cccagccttg atccattccg
     5881 tccggaaata attttgaatg gcaaaaggcg cgatggtgac gcctgtcagg ctcacgccga
     5941 tggaagaaaa caaatctttt actttctgcg gctcggaccg gggtgcgctg tacaccatga
     6001 cggcgtactt tggaatgccc tggtcgttga ttttgccctg aacttcgtaa tcaaaaaaag
     6061 cttccttttc atcgatggga ttttcttttt tggccgccca gtagatgacg ttttccattt
     6121 gatttgcggg aacaatcggt atcttcaaac ggaatacatt cacttccgcg gcggacatca
     6181 tcgcccagat atcgcaatca tccaacgatc cggcaaatgc cgtaagcgat gatttgagca
     6241 aggtattgaa ttcaggggat ttttttgaaa tcccctcgcc gagtttcaca accttttgat
     6301 cgaccagcag ggatttgccg tcagaagttt tcctcatttt gaccatcgtg acggcattgg
     6361 ccccgatatc cacgcctacc tgaaaaggcc gcttcccatt gaaaaaacgg aataagttgg
     6421 cggttatctt caaggaaggc tttttcttga aagaaatatt cggaccgctg cccggcgtcg
     6481 ccggaaaagg cggtttcgcg ccgcgaataa catttaacaa cttttccgtt gagttaattt
     6541 cttttgcctt tgccaaggag ctcacccaaa aaacaacctt ttttgcacca aaccgccacc
     6601 caaatatctc caataaatgt tgcttctacc ccaaaccata gtttactttt tataaatgct
     6661 ttttttcata caatacttcg ataattatta caaatgttta tatttggctt tgcatacgac
     6721 gaaagaactt gaaacgccta tcggattttg ttaaaaagca acgcctcaca aatac
//
LOCUS       DAOSRM010000102         6656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_10621_length_6656_cov_101.316316, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000102 DAOSRM010000000
VERSION     DAOSRM010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10621_length_6656_cov_101.316316"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLA58_08970"
     CDS             complement(<1..554)
                     /locus_tag="PLA58_08970"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOR62963.1"
                     /translation="MPKIFFEAVAGGKKPYVASLRKLLTRFTSPFEAGDRVGIKLHWG
                     ERGNKGYLPPDYAREIVVWLRAGKIKPFIFDTTVLYSGGRRNAADSLKTAADHGYTPD
                     FLGCPVIIADGPDGRNVVSLPAGYRHFKTVQVGSVFEQAKGFVIFSHFKGHMVSLFGG
                     AIKNISMGFASRAQKQRMHADYHPV"
     gene            complement(615..1358)
                     /locus_tag="PLA58_08975"
     CDS             complement(615..1358)
                     /locus_tag="PLA58_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR62964.1"
                     /translation="MRSLFNIIRVLWLYLWAIIATLVLAVPIIAAGLLSRTGNLAFSL
                     SKIWAWTMLAVSFVRAEIKNRERIRPATSYIIISNHQSLYDILALVTTLGIQYRWFIK
                     KEILKVPLFGYGLYASRNIFIDRSNTARAIESINRGIDRLPPGVSIMVFAEGTRSPDG
                     RIHEFKKGGFVTAVRRNIPILPVTVNGSRRILPKGSLVVRPGKIQVVVGEPIDAGNYT
                     QESVDALIDETRRAVIANFDPGYAGHFLD"
     gene            1580..2503
                     /locus_tag="PLA58_08980"
     CDS             1580..2503
                     /locus_tag="PLA58_08980"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF006143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOR62965.1"
                     /translation="MELKDIKKVLILGAGAMGQQIGFVCAMHGYRVTYYDISDEVLKT
                     SMKRVEKLGSAFFVPVGRITTEELKEIMSRISATSDPAQAACDADLISESVPEDPQIK
                     ASVFAQFNKLCPERTIFTTNTSLLMPSKFAAETGRPEKFLALHFHDLRANNVADVMPH
                     PGTDPAVTELVRQFAVSIGQVAIMLRKESNGYVFNAMLSSICTAAMTLVTSGVASIED
                     VDRAWMGVMRMPLGPFGMMDQMGLKTVWHVNDYWANHAQEIPANQIKVNADFLKAYVE
                     KGELGAVTKKGFYTYPNPAYARPDFLAGEKK"
     gene            complement(2811..>3507)
                     /locus_tag="PLA58_08985"
     CDS             complement(2811..>3507)
                     /locus_tag="PLA58_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOR62966.1"
                     /translation="KELKQPMSLYNPYQKEVLEASLWLSEHGYFGSLRGTGGNVSARI
                     TGSDCMAITPSSVKYSDLAPSDICIVDFAGAVVEGKYAPSIEAGMHAAVYRERPDVNA
                     VVHTHQLYGSVFAALNQPIPVLFDEVAFALGEKVEVAPYALSGSTELAANVAGLLANH
                     ANAYIIQNHGILALGKNMDKALLAAELLEKIAQIYCLALSTGKPVTLLPDATREMARG
                     LRDYDVEAARKKS"
     assembly_gap    3508..3607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3608..6273)
                     /locus_tag="PLA58_08990"
     CDS             complement(<3608..6273)
                     /locus_tag="PLA58_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR62967.1"
                     /translation="MAKNYLMAIDVGGGSGRCLLLDPETGKTVSAKRNWTHPTAPGTS
                     GLGNLIDTNGLLAKIAEASREVLLKAGAEPNRIAGIAVGGMRNTIVALDRQNNVLLAT
                     PNRDARALGEGMMLGAERGSEVHAVSGHWPTPLFLGTRLLWMKNNAPDLFRQTAAALS
                     VTDWLAFVLSGKMAAERSQAGETLLFDLQKRSWAFDLIDSLGFDRSLFPEPVDSGAPI
                     GSLTRQAADLLGLVPGIPVAAGGADTQCGLLGAGAVKDGDLAVIAGTTMPLQLVTGQL
                     ILDPTGRLWSGLHVVPGRYVLESNGLVTGDVIDWFAHLLYPDSPDGTLSLFAEAEKSV
                     PGAMNIYSTFGTALFDGRVVGFPVGNLTFSHMITGDASRSRRHIARALVEGIAFSVRI
                     NLEQLALVSGREIRELRVAGGMSQSPLFSRIVANVTNAPVSVPPTAEVTSVGAAVLAG
                     VGAGIFADPAAGAEKVASALTRYQPDAAGEKYRNFYTGWRQAFDKRADTDAHVGNLLI
                     NAMFEPAAAAGKAADPSFSPNILVTASMDETAIRELEAIGKVTYAEWREKQKLYDGGA
                     ELAGALAGTQIFITEMDVVDFPAIREARSLRAIVTCRGNAVNVDLAAATAYGIPVINT
                     PGRNADAVADLTIALMVMLSRKMPGSFDFLKHGQVQSGDMAKMGEAYLKFQGKELWRK
                     TVGLVGMGNVGACVAARLARFGAKVVFADPMVAAGEGALLGARKVSFDELLAVSDIVS
                     LHAPAIEATRGMMNREAFGRMKPGAFFVNTARASLVDEAALLEALTSGRIAGAALDVF
                     PTEPPGTDDPIVSHPNVIATPHLGGNTTEIAAHQGAIAVDQIQKLLAGEKPDYLLNPA
                     VLDIFSWTAPRPEPDEKKLEELARNKRPSMTS"
BASE COUNT         1333 a         1910 c         1981 g         1332 t
ORIGIN      
        1 acgggatggt aatcggcatg catccgctgt ttctgggcgc gggaggcaaa tcccatggag
       61 atgtttttga tggcgccgcc aaacagagaa accatgtgcc ccttgaaatg ggaaaaaatc
      121 acgaagcctt ttgcctgctc gaagacggat ccgacctgaa cggttttaaa gtgtcggtac
      181 ccggcgggaa gagagacgac atttcttcca tccgggccgt cggcgatgat gaccggacat
      241 ccgagaaagt ccggcgtgta gccgtgatcc gcggcggttt tcagggaatc cgcggcgttt
      301 cgtcgcccgc cggagtagag aaccgtggtg tcgaaaataa acggtttgat tttccccgcg
      361 cgaagccaga cgacgatttc ccttgcgtag tcgggaggca ggtagccttt gttgccccgc
      421 tcgccccagt gaagcttgat tccgacgcga tcgcctgctt cgaacggcga cgtgaaccgg
      481 gtcagcagtt tccgcagaga agcgacgtaa ggtttttttc cgccggccac ggcttcaaaa
      541 aatattttcg gcatgatcag ggtctctgtc cttcgcaggc aatgccggcg tgaaacagcg
      601 gacgggattg tccgctagtc gaggaaatgc ccggcatagc cgggatcaaa attcgcgatg
      661 acggcccggc gcgtttcgtc aatcagcgcg tcaacgctct cctgggtata gtttccggca
      721 tcaatgggtt cgccgacgac gacctgaatt ttccccggcc ggacgaccag gctgcccttc
      781 gggagaatgc gccggctgcc gttgacggtc accggcagga tgggaatatt tcgccgcacg
      841 gcggtgacaa atcctccctt tttgaattca tgaatccgcc cgtcaggcga gcgggttcct
      901 tcggcaaaca ccatgatgct cacgcccggg ggcaggcggt cgatgccgcg gttgatgctt
      961 tcgatcgccc gggctgtgtt ggagcggtcg atgaaaatat tgcgcgacgc gtaaagcccg
     1021 tatccgaaaa gcggcacctt gagaatttct tttttgatga accaccggta ctgaatgccc
     1081 agcgtggtga ccaaagcaag gatgtcgtaa agcgactgat gattggaaat gatgatgtag
     1141 gaggtggcgg gccggattct ttcccggttt ttgatttccg cgcggacaaa agaaacggcc
     1201 agcatggtcc aggcccagat cttcgaaagg gaaaaggcga gattgccggt tcggctcaaa
     1261 agccccgcgg caatgatggg gaccgccagg acgagcgtgg cgatgattgc ccacagataa
     1321 agccacaaaa cacggatgat gttgaaaagg gagcgcatcg ggtcaatcct tactctgatt
     1381 tcggaactgc ggccgccgtt ttgattgatt tctgatcctt ttgtgtgcgg attttagaaa
     1441 acgcgccccg ggatgtcaag aaaatcctta aaccggaaac aagaggcgcg aaacggtcat
     1501 gaaaagggct gaactccggc tgaacctgtg ctataggatc ccggcacagg aaaattagcc
     1561 tcaaggaaag ggaaaattta tggaactcaa agacattaaa aaagtgctga ttctgggcgc
     1621 gggcgccatg ggccagcaga tcggcttcgt atgcgccatg cacggctacc gcgtgaccta
     1681 ctatgacatc tcggacgagg ttttgaagac atccatgaag cgcgtggaaa aactcggctc
     1741 cgcctttttt gttcccgtgg gcaggatcac gacggaagag ctcaaggaaa tcatgagccg
     1801 catatcggcg acatccgacc cggctcaagc ggcatgcgac gcggacctga tcagcgaatc
     1861 cgtgcccgaa gacccgcaga tcaaggcgtc ggtcttcgcc caattcaaca aactgtgccc
     1921 ggagcggacg atctttacca ccaacacgtc gctcctcatg ccctcgaaat tcgccgcgga
     1981 aaccggccgg ccggaaaaat ttctcgccct gcattttcac gatctgagag ccaacaacgt
     2041 ggcggacgtc atgccgcatc cgggaacgga tccggcggtg acggagctgg tgcgacagtt
     2101 tgccgtgagc atcggccagg ttgccatcat gctccgcaag gagagcaacg gctacgtgtt
     2161 caacgccatg ctctccagca tctgcacggc cgccatgacg ctcgtgacgt ccggtgtggc
     2221 cagcatcgag gacgtggacc gggcctggat gggggtgatg cgcatgccgc tggggccttt
     2281 cggcatgatg gatcagatgg gtctgaaaac ggtctggcac gtcaacgact actgggcgaa
     2341 ccatgcccag gaaattccgg ccaaccagat taaagtgaac gctgatttcc tcaaagcgta
     2401 tgtggaaaag ggtgaactcg gggccgtgac gaagaaggga ttttatacct atccgaatcc
     2461 cgcttacgcc cggccggatt ttctcgcggg agagaaaaaa taacgaagcc cttcgccgcc
     2521 gtgaaagctt tgcaaaactc ctttttcgtc ataccgtcga aagagactgt gccgcaatag
     2581 gggaaattac caaagagttg tcataccggc aaaagccggt atccagacgt cgtccccgcg
     2641 gagcctgtcc tcgagaagtc ggggggcgaa aaaccagttc ctttattcct ggattccgga
     2701 tcgcgctgcg cttgcccgga atgacggctt tgacagttct gcaatgctct tgctctgtgc
     2761 agaaagagat gaaaaatctt ttttcgtctt tctttcgggc acggaaagga ttacgatttt
     2821 ttccgggccg cctccacgtc gtagtccctg aggccccgcg ccatttcccg tgtggcgtcg
     2881 ggaaggagag tgaccggctt tcccgtggac agcgccaggc agtagatttg cgcgattttt
     2941 tccagaagtt ccgcggccag cagcgccttg tccatgtttt tccccagtgc gagaataccg
     3001 tggttctgga tgatgtaagc gttggcatgg ttcgcgagca ggcccgccac attggcggcc
     3061 agctccgtgc tgccggagag ggcgtagggg gccacttcca ccttctcgcc gagagcgaag
     3121 gcgacttcgt cgaacaggac gggaatgggc tgattgagcg cggcgaagac gctgccgtaa
     3181 agctgatgcg tgtgaaccac cgcgttgacg tccggccgtt cgcgataaac ggccgcgtgc
     3241 attccggcct cgatcgaagg ggcgtatttt ccttcaacga cggcgcctgc aaaatcgacg
     3301 atgcagatat cggacggcgc gaggtccgaa tatttcacgg aggacggtgt gatggccatg
     3361 cagtcgctgc cggtgattct cgccgacaca ttgccgccgg tgccccgcag agagccgaaa
     3421 tagccgtgct ccgaaagcca gaggctcgcc tccagcactt ccttttgata tggattatat
     3481 aagctcatcg gttgttttaa ctcctttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnntac gacgtcatgg agggccgctt gtttctggcc agttcctcca gcttcttttc
     3661 gtccggttcg gggcgcggcg ccgtccagga aaaaatatcc aggacggccg gattgagcag
     3721 ataatccggt ttttcccccg ccagaagctt ttgaatctga tcgacggcaa tggcgccctg
     3781 atgcgccgca atttctgtgg tgttgccgcc cagatgcggc gtggcaatca cgttggggtg
     3841 tgagacgatc gggtcgtccg ttcccggcgg ctcggtcggg aaaacgtcca gcgcggcccc
     3901 ggcgatcctg cccgatgtca gcgcctctaa aagagctgcc tcgtccacca gcgacgcgcg
     3961 ggcggtattg acgaagaagg cgcccggctt catccgcccg aaggcttccc ggttcatcat
     4021 tccccgcgtc gcttcgatgg ccggcgcgtg aagactgacg atatcgctta cggccagcag
     4081 ttcatcgaag gacaccttgc gcgcgcccag cagggcgccc tccccggcgg cgaccatcgg
     4141 gtcggcaaaa acgaccttcg ctccgaagcg cgccagcctt gccgcgacgc aggccccgac
     4201 attgcccatc cccaccagtc cgacggtttt cctccagagt tccttcccct ggaatttcaa
     4261 ataggcttct cccattttcg ccatgtcgcc cgattggacc tgcccgtgtt tcaggaagtc
     4321 aaacgatccg ggcatcttgc gcgacagcat caccatcagg gcgatggtca gatccgcgac
     4381 ggcatcggcg tttcttcccg gcgtgttgat gacggggatg ccgtaggcgg tggcagccgc
     4441 gagatcgacg ttgacggcgt ttccgcggca ggtgacgatg gcccgcaggc tgcgcgcctc
     4501 gcggatggcc gggaaatcca caacgtccat ttcggtgatg aaaatctgtg tgccggccag
     4561 cgctccggcc agctccgcgc ctccgtcgta gagcttctgc ttttcccgcc attccgcgta
     4621 cgttaccttc ccgatggctt cgagttcgcg gatcgcggtt tcgtccatcg aggcggtgac
     4681 aaggatgttg ggcgaaaaag acggatcggc cgccttgccc gcggccgcag cgggttcgaa
     4741 catggcgttg atcagcaggt tcccgacgtg ggcgtcggta tcggcgcgct tatcgaaagc
     4801 ctgccgccag cccgtgtaaa agttccggta tttttccccg gccgcgtccg gctgatagcg
     4861 ggtcagggcg gaggctacct tttcggcccc ggctgccgga tcggcaaaga ttcccgctcc
     4921 gacgcccgcc agcacagccg cgcctacaga cgtcacttcc gcggtgggcg ggacggaaac
     4981 gggtgcattc gtcacgtttg cgacaatccg cgaaaacagc gggctttggg acatgcctcc
     5041 ggcgaccctc agctcgcgga tttccctgcc ggagaccaag gcgagctgtt cgaggttgat
     5101 gcggacggaa aaggcgatcc cctccaccag ggcgcgggcg atgtgcctgc ggctgcggga
     5161 agcgtcgcct gtgatcatgt gggaaaaggt gagattgccc acggggaagc cgacgaccct
     5221 tccgtcgaac agggcggttc cgaaagtgga ataaatgttc atggcgcccg gaacggattt
     5281 ttccgcctcc gcaaagaggc tgagcgtccc gtcgggagaa tccggataga gaagatgggc
     5341 aaaccagtcg atgacgtcgc ccgtgaccag gccgttgctc tccagaacat accggcccgg
     5401 cacgacgtgc agaccgctcc agaggcggcc ggttggatcc agaatcagct ggccggtcac
     5461 cagttgcagg ggcatggtgg tcccggcgat gacggccagg tcgccgtctt tcaccgcgcc
     5521 cgcgccgagc agaccgcact gggtgtcggc cccgcccgcc gccacgggga tgccgggcac
     5581 aagacccagc agatcggccg cctgccgggt gagactgccg atgggcgcgc cggaatccac
     5641 gggctccgga aagagcgacc ggtcaaatcc cagcgaatcg atgagatcga aagcccaact
     5701 gcgcttttgc agatcgaaaa gaagcgtttc ccccgcctgg gagcgttcgg cggccatctt
     5761 tcccgacagg acgaaggcca gccagtccgt gacggacagg gccgcggcgg tttgccggaa
     5821 caggtcgggc gcgttgtttt tcatccacag caggcgcgtg ccgagaaaca gcggcgtcgg
     5881 ccagtgcccg gagacggcgt ggacttcact tcccctctcg gcccccagca tcatgccttc
     5941 gcccagggcc cgggcgtccc ggttgggggt tgccagaaga acattgtttt gccgatccag
     6001 cgccacgatg gtgttgcgca tgccgccgac ggcgatgccc gcgatccggt tcggttccgc
     6061 cccggccttg agaaggactt cgcgggaggc ttcggcaatc ttcgcaagca gaccattcgt
     6121 gtcgatcagg ttgccgagtc cgcttgtgcc gggcgcggtc ggatgcgtcc agtttcgctt
     6181 tgccgatacg gttttgcccg tttccggatc cagaaggagg cagcggccgg agcctccgcc
     6241 cacatcgatg gccatcagat agttttttgc catgtttgac gctcccagat ttgatgcgcg
     6301 aagtatagaa agccggtccg gtcaagtcaa gcctttttgc cttctccgta acgtagtgcg
     6361 gggttttaac cccgccatgt cttctgggtt atgtagtgcg ggtctttaga cccgccaaaa
     6421 ccgcgcagct aaagctacgc actacatcat attcgtaaag tagtgcgaat ttttaaatcc
     6481 gctatgtcct ccagattatg tagtgcgggg ttttaacccc gctaattccg ttcccaccat
     6541 caaaatcaaa acgtcacggt tttaacatcc agcgccagcg atcccaggta ccggtaatcg
     6601 ccataatggc cgactaaccc tttccggacg gggttgttga aaatatactt caacac
//
LOCUS       DAOSRM010000103         6524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_10887_length_6524_cov_105.021796, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000103 DAOSRM010000000
VERSION     DAOSRM010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10887_length_6524_cov_105.021796"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1192)
                     /locus_tag="PLA58_08995"
     CDS             complement(224..1192)
                     /locus_tag="PLA58_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOR62968.1"
                     /translation="MRWTGKKVGLALGGGGVRGLAHIGVIQVLEKEGVEIDLIAGTSA
                     GALIGGAYATGMTTREITAKVDAYLKSSEFEESAIKSMGLSFSTGPKSFFQKAQTFAR
                     NQYYLVKALFSSSILPAEDFQSLINYLLPDVDIRATRIPFYAVSTDLITGKQVVLSEG
                     SLRQAVLASASVPGAVEPTRLGEWLLADGGVTSLVPVHAARAAGADIVIAVVVDRDLP
                     QETDIETAKDVLYRAGEITANTLEAAELQSADVIIRPAVGDLHWADFSRSHTLIRIGE
                     EAARESLEKIHASLPLPRRFARFARHLLDRAQNRGPRHIGSGPAPL"
     gene            complement(1337..2698)
                     /gene="mgtE"
                     /locus_tag="PLA58_09000"
     CDS             complement(1337..2698)
                     /gene="mgtE"
                     /locus_tag="PLA58_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOR62969.1"
                     /translation="MQEVKKKEQTPLATLKDLISRNREFDIIDLFSDMHPADIADLID
                     ELDEEDRLHLFTLLDVDKASDVILELSDNSREQLIEDLSNEQLTDIIEEMESDDKADI
                     IAELSDDQAKAVLDAIEPEESQEVKELLRHPEDTAGGIMQSELVSVGKQATINDAFQA
                     VVDAKDDMENIHNIFVVDDENKLVGTVPLQRLITMKRFTPIVDVIDGNIPSVRADMDQ
                     EEVARLFKKYDLVSAGVVDADNHLLGRITIDDVVDAIQEETSEDIYRIAGLGYNDTIF
                     NKTIDSVKKRLPWLYLNLMTALTSVLVIGFFEDTIKIMVALVFFMPVIAGLGGNAGGQ
                     TLALIIRGIALGEITFENAKRVLARQIAVGATNGVAVGLVIALIAYFWKGIPILGLVI
                     GLAMIISVFAGTMVGVLIPLALKRLNFDPALGSNILLTAFTDAFGFFSYLTLATIFLK
                     LLM"
     gene            complement(2925..3701)
                     /gene="truA"
                     /locus_tag="PLA58_09005"
     CDS             complement(2925..3701)
                     /gene="truA"
                     /locus_tag="PLA58_09005"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOR62970.1"
                     /translation="MNKYKIVLEYDGTNYSGWQAQKNARSIQETLIGAARKFLGAPVE
                     LQGAGRTDAGVHALGQVAHLECANKVNGDALRMGLNDLLPAGINILSVESAHPRFHAR
                     HSAVSRSYLYLIATRRTAFGKKYVWWIKDSLHIGKMRQALKVFEGFHDFSSFADKRME
                     KGASTTVQLESAQLMEFENIIAVRLVASHFLWKMARRIVGMTVEVGRGRQTAEDLAGM
                     LRSFSDVPARHTAPPSGLFLEKVLYEGDRLPDIQPPVHLL"
     gene            complement(3698..5050)
                     /locus_tag="PLA58_09010"
     CDS             complement(3698..5050)
                     /locus_tag="PLA58_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family N-terminal domain-containing
                     protein"
                     /protein_id="HOR62971.1"
                     /translation="MGRIYRLAVIFFFILSGCVSAPDTIRDVRELRQDHAAYLTDISG
                     KADPLPAALQARMDEDYNVIYFSVWHQTRPFHAWEERVRHDFRRYALRPGYGENKKPH
                     PSSWLKKLQRNADLEGYPNAPARGITTRNTNLRDLPTNSPHFTSANGDSSAWPFDNLQ
                     RSSVPAGTPVFVCHVSEDKAWALVETSFTYGWMPAEDFARVNEDFAKTWESGRYAVIL
                     RDSTSVLDRNGRFLVKASVGHLFPFSAAQDGKIQIFVAVADQNRQAVIRQGFVPAEAA
                     AAKPLRFTPANAAKIANEMIGEPYGWGGLYGRRDCSAMTRDFFTVFGIWLPRHSEDQV
                     KEAGAYINLRGLSPEEKERIIMEKGVPYLSLLWRKGHVMLYIGAPQGRALIFHNIWGI
                     RTKDLQGREGRKIIGQAVITGLTPGRELSDMDTAAGSLLDNIAAMNILSADVREAPAP
                     "
     gene            5243..5791
                     /locus_tag="PLA58_09015"
     CDS             5243..5791
                     /locus_tag="PLA58_09015"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOR62972.1"
                     /translation="MQDFVPKKIFFTKGVGTHKDELHSFERALRNAGIEKCNLVQVSS
                     IFPPGCKMIPKSQGLKSLTPGAITYCVMSRCCSNEPKRLLAASVGCAIPADKTSYGYI
                     SEHHAFGMTEKQTGDHAEDLAAAMLASTLGIDFNVDESWDEKKEIFKISGKIVRTLNV
                     TQSKICMDNHYTTVVAAAVFVF"
     gene            5853..>6524
                     /locus_tag="PLA58_09020"
     CDS             5853..>6524
                     /locus_tag="PLA58_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR62973.1"
                     /translation="MKRDIPDLKQQDVGGAELPYLYYPGGEKQMLFCHATGFLPWLWH
                     PIIEEFSREYTIWAPYICNYRSCDPHQGGLKWNIIAEDLGAFCRAQNIENHVQVGHSM
                     GATVSTIAASLFGVRPRGMVLIEPIFLPEEFYSLTPSVEGHPLASKSIKRLNYWQSEE
                     DAHAYLKSRSFFTTWDEEILRLYKRFGMEKMPEGDLRLACAPESEAAMFMGGWCCNPW
                     PLLEKI"
BASE COUNT         1349 a         1867 c         1907 g         1401 t
ORIGIN      
        1 agtggctcaa ttcctgacga ccatttttgg ctcaatttat cacgaccgga aacagaggtg
       61 tggcttttaa aaaaataccg ggtttgcagg gaaaacgacc acggccgtgt atcggcttcc
      121 ttgtcatgaa tcccggcggc tgacgccggg ccggacatcg gtgaaaaaag caaagcgaag
      181 gcgagaagcg ccaggaggca ggaatggatt ggaccgggta atttcataaa ggagccgggc
      241 ctgaaccgat atgccggggg ccgcgattct gcgcccggtc cagcaagtgc cgggcaaagc
      301 gggcaaagcg ccggggcagc ggaagggagg cgtgaatctt ttccagcgat tcgcgggcgg
      361 cttcttctcc gatccggatg agggtgtgcg accggctgaa atcggcccag tgcaggtccc
      421 ccacggcggg gcggatgatg acatccgcgc tttgaagttc ggcggcctcc agcgtgttgg
      481 cggtgatctc gccggcccgg tacaaaacgt ctttggccgt ttcgatgtcc gtttcctgcg
      541 gcagatcgcg gtccaccacc acggcgatga caatatccgc tccggccgcg cgcgccgcgt
      601 ggacggggac caggctggtc acgccgccgt ccgccagaag ccactcgccc agccgggtgg
      661 gttcaaccgc tccgggaacg gaagcgctgg ccagcaccgc ctgacgcagg gacccttcac
      721 tcaagacgac ctgtttgccg gtgatcaaat ccgtggagac ggcataaaag gggatgcggg
      781 tggcgcggat gtccacatcc gggagcaggt aattgatgag ggactgaaaa tcctcggcgg
      841 gcaggatgga agagctgaac agggccttga ccagataata ttgattccgg gcaaaggttt
      901 gcgctttctg aaaaaaactc ttcggccccg tggaaaacga caggcccatc gatttaatgg
      961 cggattcctc gaattccgaa ctcttcagat aggcgtccac ttttgccgtg atctcgcggg
     1021 tggtcatacc cgttgcgtag gcgcccccga tcagcgcgcc tgcgctggtc ccggcgatca
     1081 gatcgatttc aacgccttcc ttttccagaa cctggatcac gccgatgtgc gccaggccgc
     1141 ggacaccgcc gccgcccagg gcaagtccaa ctttttttcc ggtccatctc atggtgtgtt
     1201 ccgctccatt cccacgacgg atacgctgat ggctgtattg taagcaatgc cgcttgcgga
     1261 tgtcaactga ataccggcca ttgccgcttt ccggtcgggc agactttgat catggatcaa
     1321 acggaacagc cgggcctcac atcaggagtt tgagaaaaat ggttgccagc gtcagatacg
     1381 agaaaaatcc aaaagcgtcc gtaaaggccg tcagcagaat attggaaccc agcgccgggt
     1441 caaaattcaa acgcttgagg gccagcggaa tcaggacgcc caccatcgtg ccggcgaaca
     1501 cgctgatgat catggccaga ccgatcacca gtccgaggat gggaatgcct ttccagaaat
     1561 aggcgatcag cgcgatcacc agtcccacgg cgacgccgtt ggtcgctccc acggcaatct
     1621 gccgggccaa tacgcgtttg gcgttttcaa aggtgatttc ccccagagcg atgccgcgga
     1681 tgatcaacgc cagcgtctgg cctcctgcgt tgccgccgag tccggcgata acgggcataa
     1741 aaaagaccag ggcgaccatg atcttgatgg tgtcttcaaa aaagccaatg accaggaccg
     1801 acgtcagcgc cgtcatcaga ttgaggtaaa gccagggcag gcgtttttta acggaatcga
     1861 tcgttttgtt gaaaatggtg tcgttatatc ccaaaccggc gatgcggtag atgtcctccg
     1921 aggtctcctc ttggatggca tccacgacgt cgtcgatggt gatgcggccc agcaggtgat
     1981 tgtcggcgtc caccacgccc gcggagacca ggtcgtattt cttgaacagg cgcgccacct
     2041 cttcctgatc catgtccgcc ctgacgctgg gaatattgcc gtcgatgacg tccacgatcg
     2101 gcgtaaagcg cttcatggtg atcagtctct gcaggggcac cgtcccgacc agcttgtttt
     2161 catcgtccac gacaaagata ttgtggatgt tttccatgtc gtccttggcg tccacgacgg
     2221 cctggaaggc gtcattgatg gtggcctgtt tgccgacgga gaccagctcc gactgcatga
     2281 tgccgccggc cgtgtcctct ggatgccgga gaagctcctt gacctcctgg gattcttccg
     2341 gctcgatggc gtcgagaacg gccttggcct gatcgtccga cagttcggcg atgatgtccg
     2401 ccttgtcgtc cgactccatt tcctcgatga tgtcggtgag ctgttcgttg gacaggtcct
     2461 cgatgagctg ttctctcgag ttgtccgaca gttccagaat aacgtccgag gctttgtcca
     2521 catccagcag ggtgaagaga tgcaggcggt cctcttcatc gagttcatcg atcagatcgg
     2581 cgatgtcggc cggatgcatg tcggagaaaa gatcgatgat gtcgaactcg cggttgcgac
     2641 tgatcaggtc tttgagggtg gccagcggag tctgctcttt tttcttaacc tcttgcatag
     2701 ggctgcctga cgtatcttgg gttgaagggt gtctggtatt gattcgattc agcaggaaag
     2761 gcaacaatcc tgatgtccgt ccgctgggcc agggactctt tttaaaagcg tatgacgcaa
     2821 tcgaaagcgt cttcccggcg acgggaatat aggaaagttc cctcagtatg tcaatgtttt
     2881 tctttgaaaa tatggattca ggaccgtttg ggcgggaccg gggtttacag cagatgaacc
     2941 ggcggctgaa tatccggcag ccggtcgcct tcgtataaaa ctttttccag aaaaagcccg
     3001 gagggagggg ccgtgtggcg ggcaggaacg tcggaaaaag aacgaagcat cccggcaagg
     3061 tcttccgccg tctgacggcc gcgtccgacc tcaacggtca tgccgacgat gcgccgcgcc
     3121 attttccaca gaaaatggga ggcgaccagg cgcaccgcga tgatgttttc gaattccata
     3181 agctgagcgg attccagttg caccgtggtg gaagcgcctt tttccattcg tttgtcggca
     3241 aaggaggaaa agtcatgaaa gccttcaaaa accttcagcg cctgccgcat tttaccgatg
     3301 tggaggctgt ctttgatcca ccagacatat tttttgccga aggcggtgcg gcgggtcgcg
     3361 atcagataca ggtagctgcg gctcacggcc gaatggcggg cgtgaaaccg cgggtgggcg
     3421 ctttccacgc tcagaatatt gatgcctgcc ggcagcagat cgttcagtcc catccgcagg
     3481 gcgtcgccgt tcaccttgtt ggcgcattcc agatgggcga cctgccccag cgcgtggacg
     3541 ccggcgtcgg tgcgccccgc tccctgcaat tccaccggag cgcccagaaa tttccgggct
     3601 gctccgatca gcgtttcctg gatgctcctg gcgttcttct gcgcctgcca gccgctgtag
     3661 ttggtgccgt catactcgag aacgattttg tatttgttca tggcgccggc gcctcccgga
     3721 cgtccgcgga caggatgttc atggcggcga tgttgtccag caaagagccg gctgccgtgt
     3781 ccatgtccga aagctcccgt cccggcgtca gtccggtaat caccgcctgc ccgatgattt
     3841 ttcgcccctc acgcccctgc agatcctttg tccgaatgcc ccagatgttg tggaagatga
     3901 gcgcccggcc ttgcggagcg ccgatgtaca gcatcacgtg cccctttcgc cacagcaggc
     3961 tcaggtacgg gacgcctttt tccatgataa ttctttcctt ttcttccggc gacaggcccc
     4021 gcaggttgat atacgcgccc gcctccttca cctgatcttc cgagtgccgc ggcagccaga
     4081 tcccgaagac ggtgaaaaaa tcgcgggtca tggccgagca atcgcgcctg ccgtaaaggc
     4141 cgccccagcc gtagggttct ccgatcattt cgttggcgat ttttgccgcg ttggccgggg
     4201 taaatcgcag cggcttggcc gcggccgctt ccgccgggac aaacccctgc cggatcaccg
     4261 cctgccggtt ttgatccgcc accgcgacga agatttgaat ctttccatcc tgcgctgcgc
     4321 tgaaagggaa gagatggccc acggacgcct tcacgaggaa ccgaccgttc cggtccagaa
     4381 cggacgtgga atcacgcaga atgaccgcgt agcgtcccga ctcccaggtt tttgcaaaat
     4441 cttcgttgac ccgggcaaag tcttcagccg gcatccagcc ataggtaaag gaggtttcca
     4501 ccagcgccca ggctttgtcc tcactgacat ggcagacaaa aaccggcgtg ccggcgggca
     4561 cggacgaccg ctgcagattg tcgaaaggcc aggcggaact gtccccgttt gcgctcgtga
     4621 agtgggggga attcgtgggc agatcccgca aatttgtgtt ccgggttgtg atcccgcggg
     4681 ccggcgcgtt ggggtagccc tccaggtcgg cgttgcgctg cagttttttc agccacgagg
     4741 agggatgcgg ttttttattt tcaccgtagc cgggcctgag agcgtatctt ctgaaatcat
     4801 ggcggactct ttcctcccag gcgtgaaaag gacgggtctg gtgccacacg gagaagtaaa
     4861 tgacgttgta atcttcgtcc atccgcgctt gcagggcagc ggggagcgga tcggccttcc
     4921 cggaaatatc cgtgaggtag gcagcgtggt cctgacgcag ctcccggaca tcccggatgg
     4981 tgtcgggcgc ggaaacgcaa ccggataaaa tgaaaaagaa aatcactgcc agtctgtata
     5041 ttctgcccat gaggacatcc tttacggaag atgctgttcc ctatagtctt tttttgcgtt
     5101 tcccgcaaga taaacgcctt tcgggtgtcc ccatcatttt cttttgcatt cttgaaaatt
     5161 ttgtgtaaat tacggccatg attttcatgg gtgtttgatc cacgtcccgg aggcaccaca
     5221 tcccattcca aaggagagat cgatgcagga tttcgttccc aaaaaaattt ttttcaccaa
     5281 gggtgtcggc acgcacaagg atgagctgca ttcctttgaa cgcgccctgc gcaatgccgg
     5341 cattgaaaaa tgcaatctcg ttcaggtgtc cagcattttt cctcccggct gcaaaatgat
     5401 tcccaagtcg caggggctca agtcgctgac gccgggcgcc atcacctatt gcgtgatgag
     5461 tcgctgctgc tcgaacgagc ccaagaggct gctggccgcg tcggtcggct gcgccatccc
     5521 cgccgacaaa acctcctatg ggtacatcag tgaacaccat gccttcggca tgacggaaaa
     5581 acaaaccggc gaccacgccg aggacctggc cgcggccatg ctggcttcca cgctggggat
     5641 cgatttcaac gtggatgaga gctgggatga aaaaaaggaa atcttcaaaa tcagcggcaa
     5701 gatcgtccgg acgctgaacg tgacccagtc gaaaatctgc atggataacc attacacgac
     5761 ggtggttgcc gccgccgtgt ttgtcttctg atcaacggat cccggtcgcg ccccgcgagc
     5821 ggaagattca ccgggaaagg attggattac cgatgaaaag agacattccc gacttgaagc
     5881 aacaggacgt gggcggcgcc gaactgccct acctgtatta tccgggaggc gaaaagcaga
     5941 tgctcttctg ccacgccacc ggcttcctgc cctggctgtg gcacccgatt attgaagaat
     6001 tcagccgcga atatacgatc tgggcgcctt acatctgcaa ttaccggtcg tgcgacccgc
     6061 atcagggcgg cctgaagtgg aatatcatcg ccgaggacct gggcgcgttc tgccgggcgc
     6121 aaaacattga aaatcacgtg caggtcggtc attccatggg cgccacggta tcgacgatcg
     6181 ccgcttctct tttcggcgtt cgtccgcgcg gcatggtcct gatcgagccg atttttctgc
     6241 cggaggaatt ctacagcctg actcccagcg tcgaaggcca ccccctggcg tccaagtcga
     6301 tcaaacgcct caactactgg caaagcgagg aagacgcaca cgcttatttg aaatcccgct
     6361 ccttttttac cacctgggac gaagagattc tgcgtcttta caagcgcttc ggcatggaaa
     6421 aaatgccgga aggcgacctg cggctggcct gcgcgccgga gagcgaagcg gccatgttca
     6481 tgggcggctg gtgctgcaat ccctggccgc ttctggaaaa gatc
//
LOCUS       DAOSRM010000104         6499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_10942_length_6499_cov_109.679236, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000104 DAOSRM010000000
VERSION     DAOSRM010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10942_length_6499_cov_109.679236"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /gene="lpxB"
                     /locus_tag="PLA58_09025"
     CDS             <1..871
                     /gene="lpxB"
                     /locus_tag="PLA58_09025"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOR62974.1"
                     /translation="LVILIDFPDFNLNIAARAARKRGIRIFYYISPQVWAWREGRVKQ
                     IKKLVDQMAVILPFEVDFYAARGFDVHYVGHPLRDIVKTPFTARQARAHFGLAEDKTT
                     IGLLPGSRNSEIQKLLPEMVEAARIISRKIPGTQYILPLADTLEEKTVAGFLSDSGLK
                     VKIVAGHTYDVLACCDLAVVTSGTATLETGLMGVPMVIVYKVSLFSELIGRMIIRQQH
                     IGLVNILAGKTVAPELIQRDARGPRIASEALAILQNREKRRQIIRDLGNIRAKLGEPG
                     AARRAAQIACNMI"
     gene            880..2637
                     /gene="msbA"
                     /locus_tag="PLA58_09030"
     CDS             880..2637
                     /gene="msbA"
                     /locus_tag="PLA58_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HOR62975.1"
                     /translation="METFQRLFMMAKQYYMRFLFAAICMVIAGGLQSALPLIAKPAID
                     EIFMSKNIDALKWIPLAIIAIFLFKGLANYGQTILMSSIGLRIVTDLRNKLYEQIQKQ
                     SLSFFAEHPTGLLMSRITNDVQAVQTASSEAITALVKDTFMLIALVGVIFYMDWKLAL
                     IAMVVFPLTIYPISRFGKKMRKVTTSSQITMGTLNSLLQETISGTRIVKAFCMEKYES
                     ERFAAENERLFKYSMKAVSVNAISSPLMDFLGGLGIAAVITYGGYNVVQGHSTPGTFF
                     SFIAALLMLYEPIKRLTNVNNTINQGVAGAERIFSVIDRTPDIEDRPGAVALPKISRG
                     IDIENVTFCYETTPVLKNINLSIKAGEVIAFVGMSGGGKTSLVNLIPRFYDVTEGRIL
                     IDGHDIRDVSLQSLRGQIAIVTQQTILFNDTVRNNIAYGDIRKTDEDIYNAARAANAH
                     DFILRLPNGYDSNIGELGTKLSGGEKQRISIARALLKDAPILILDEATSSLDTEAEIE
                     VQEALDNLMRGRTTLVIAHRLSTIRNADRIIALVNGEIVEEGNHDALMEKKGEYYRLY
                     NLQFKDEGNGNDRLAKGLG"
     gene            2609..3712
                     /gene="lpxK"
                     /locus_tag="PLA58_09035"
     CDS             2609..3712
                     /gene="lpxK"
                     /locus_tag="PLA58_09035"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOR62976.1"
                     /translation="MIDWQRVWDENDSVRRISPTRSILHLGSLIYRLIVFSRNRLYDR
                     QMLKSVRLACPVISVGNLTVGGTGKTPCVIGLAKMLTRHGYRPAVLSRGYGGQHSGPV
                     RIVSDGANVEQSAAETGDEPLLIARSLPGVPVITGAKRTLTGQAAIERFNADALICDD
                     AFQHRQIFRDINIVLLDAERPLGNGHLLPRGELREPVESLRRADCIVLTRADKDGPTP
                     ARIARIAEDRGIPVFRAVHRFREIIGPDGGVLPPEHLRGKKVCAFCGIAKPASFKKIL
                     IEAEAEVVSFIDFPDHYVYNRDDLEALGWHFSAQNADCRLTTEKDAMRLEAFPDFLKM
                     LFVARVEMDIQPAKRFEDFIINRLAGASTSGKT"
     gene            3709..4596
                     /locus_tag="PLA58_09040"
     CDS             3709..4596
                     /locus_tag="PLA58_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR62977.1"
                     /translation="MKTKPSAKISITIAAAKMIPLAVRRWIANRIGGLGYCLSLKHRL
                     IALHNLMRSFPEKPLDEIVAIARASYKSFALVFAEFPEILCLDRNNIRKWVSVRGLEH
                     YEAALREGKGVVLITAHFGNWEMGMAAMALLSRPPTFVARVLDSAILEEGSVYIRSTV
                     GIGIIHKENAMRPMLRLLKQGEAVKMLIDQNVAAYEGVFINFFGRPACTTSGVALIAM
                     RSGAAVLPVFTNRMPDGTYLTEIGPKVETVHTGDRRRDVATNTQNYATAIENHIRKYP
                     EQWLWLHQRWKTKTCQAKE"
     assembly_gap    4664..4763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4868..5947
                     /gene="waaF"
                     /locus_tag="PLA58_09045"
     CDS             4868..5947
                     /gene="waaF"
                     /locus_tag="PLA58_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOR62978.1"
                     /translation="MLEAKNKLPLEGLNKILIRGTNWIGDAVMTLPAVASVRAAYPGA
                     HLSVLAKPPVSDIYKMFSAADEILPYPRKFDNAPGVLRLAYSLRPKKFDAAILLQNAI
                     EAAIIAYVAGIGVRAGYSTDGRGFLLTHPVRRSADILRVHQIDYYLEMVKALGCADVD
                     RSLRLETSLSTATTREILRKYLPENNRPLIGIAPGAMYGPAKRWLPERFAQAGESLAK
                     KLDAQVLLFGGGDDWETAELVRGQAQTDMMNLAGRTSLEDSVYLISQCRLFLSNDSGL
                     MHVAGGLNIPTVAIFGSTNPVTTSPAGERTTLVRKETPCSPCLKKTCPTDFRCMTAVT
                     VEDVVEAALTLINGTTGPRPDHRKS"
     gene            5954..>6499
                     /locus_tag="PLA58_09050"
     CDS             5954..>6499
                     /locus_tag="PLA58_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOR62979.1"
                     /translation="MKKNAAIFLDRDGTINEEVGYLDSPDKLVIFPSAVEAVRLINQS
                     GFKAIVVTNQAGVARGLFGEDLVEKIHAALRDALRQRGALLDAFYYCPHHPTEGLPPY
                     RQSCDCRKPSPGLFLQAARELNLDLSASWMIGDRYNDIEAAHRAGAKGILVRTGYGAD
                     ELSGEGPDAETPAGKPEFIADN"
BASE COUNT         1422 a         1935 c         1812 g         1230 t
ORIGIN      
        1 cctggtcatc ctgatcgatt ttcccgattt caacctcaac atcgcagccc gcgccgccag
       61 aaaaagaggc atccggatct tttactacat cagcccgcag gtgtgggcct ggcgcgaggg
      121 acgcgtcaaa cagatcaaaa aactggtgga ccagatggcc gtgattctgc cgtttgaagt
      181 ggatttttac gccgcgcgcg gattcgacgt ccattacgtc ggacatcccc tgcgcgacat
      241 cgtgaaaacg ccgttcacgg cgcggcaggc ccgggctcac ttcggccttg cggaagacaa
      301 aacgaccatc ggcctgctgc ccgggagccg gaattcggaa atccaaaagc ttctgccgga
      361 aatggtcgag gcggcgcgaa tcatttcccg aaagatcccc gggacgcagt acattctgcc
      421 cctggcggat actcttgagg agaaaaccgt cgccggcttt ctgagcgatt ccggcctgaa
      481 ggtcaaaatc gtcgccgggc acacttacga cgtcctggcc tgctgcgacc tggccgtcgt
      541 gacctccgga acggcgacgc tggagaccgg cctgatgggc gtgccgatgg tcatcgttta
      601 caaggtctcc ctcttttcgg agctgatcgg ccggatgatc atccggcagc agcacatcgg
      661 actggtcaac attctggccg gcaaaaccgt cgctccggaa ttgattcagc gagacgcccg
      721 cggcccgcgc atcgcgtccg aggctctggc cattttgcaa aaccgggaaa aacgacgaca
      781 gatcatccgg gatctgggca acatccgcgc caaactcggc gaacccggcg ccgcgagaag
      841 ggctgcgcaa atcgcctgca atatgatata ggacgaccca tggaaacgtt tcaaagatta
      901 tttatgatgg ccaagcagta ttacatgcgc tttctttttg ccgcgatctg catggtcatt
      961 gccggcggtc tgcaatccgc gctgccgctg atcgccaaac ccgccattga cgaaattttc
     1021 atgagcaaaa acatcgacgc gctcaaatgg atcccccttg ccatcatcgc gatctttttg
     1081 ttcaaggggc tggccaacta cggacagacg attctgatga gctccatcgg cctgcgcatc
     1141 gtcacggatc tgcgcaacaa actgtacgag cagatccaga agcagtctct gtcgtttttc
     1201 gccgagcatc ccacggggct gctcatgtcg cggatcacca acgatgtgca ggccgttcaa
     1261 accgcgtcgt cggaggcgat cacggcgctg gtcaaggaca cctttatgct gattgcgctg
     1321 gtcggcgtca ttttctacat ggactggaag ctcgcgctga tcgccatggt ggttttcccg
     1381 ctgacgattt atccgatttc ccgtttcgga aaaaaaatga gaaaggtgac cacctcttcg
     1441 cagatcacca tgggaacgct gaactcgctc ttgcaggaaa cgatttccgg aacgcgcatc
     1501 gtcaaggcct tctgcatgga aaaatacgaa agcgagcgat ttgccgcgga aaacgaaagg
     1561 ctcttcaaat acagcatgaa ggccgtttcc gtcaacgcca tttccagccc gctgatggat
     1621 tttctgggcg gcctgggcat cgccgccgtc attacctacg gcggctacaa cgtcgtgcag
     1681 ggccattcca cccccggcac ttttttctcc ttcatcgcgg cgcttttgat gctctatgag
     1741 ccgatcaagc gcctcaccaa cgtcaacaac accatcaatc agggcgtcgc cggcgccgaa
     1801 cgaatcttca gcgtcattga ccggacgccg gacatcgaag acagacccgg cgccgtcgct
     1861 ctgccgaaga tttcacgggg catcgatatt gaaaacgtca cgttttgtta tgaaacgacc
     1921 ccggtgctga aaaacatcaa tctatcgatt aaagccgggg aagtcatcgc gtttgtcggc
     1981 atgagcggcg gcggcaaaac gtcgctggtg aatctgattc cccgttttta cgacgttacc
     2041 gagggccgca tcctgatcga cggccatgac atccgcgacg tcagcctgca gtcgctgcgc
     2101 ggccagatcg ccatcgtgac ccagcagacc atcctcttca atgacaccgt ccggaacaac
     2161 atcgcctacg gcgacatccg gaaaacggat gaagacatct ataatgcggc tcgcgcggcc
     2221 aacgcccatg atttcatcct ccggctgccc aacggctacg acagcaacat cggcgagctg
     2281 ggaaccaaac tctccggcgg agagaagcag cgcatctcca tcgcgcgggc gctgctcaag
     2341 gacgccccga ttctcatcct ggacgaagcg acctcctcgc tggacacgga agcggaaatc
     2401 gaagtccagg aggcgctgga caacctcatg agggggcgca ccacgctggt catcgcgcac
     2461 cgcctttcca cgatccgcaa cgccgaccgg atcatcgcgc tggtcaacgg agaaatcgtg
     2521 gaggaaggca accacgacgc gctgatggag aaaaaaggcg aatactaccg gctgtacaat
     2581 ctgcagttta aagatgaagg gaacggcaat gatcgactgg caaagggtct gggatgagaa
     2641 tgactcggtt cgccgcatca gtccgacccg gtcaatcctc catctgggct ccctgattta
     2701 ccggctgatc gtcttctccc gcaaccgtct ttacgaccgg caaatgctga agtccgtccg
     2761 gctcgcctgt cctgtgatca gcgtcggaaa tctgaccgtc ggcggaaccg gcaaaacccc
     2821 ctgcgtaatc ggactggcaa aaatgttgac gcggcacgga taccggccgg ccgtcctcag
     2881 ccgcggatac ggcggccaac attccgggcc tgtccggatc gtatccgacg gagcaaacgt
     2941 agaacaaagc gccgcggaga cgggcgacga accgctgctg atcgcccggt cgctgcccgg
     3001 cgttccggtg atcaccggcg cgaagcggac gctgaccgga caggcagcca ttgagcgatt
     3061 caacgccgac gcgctgatct gcgacgacgc attccagcac cggcagattt tccgggacat
     3121 caacatcgtt ttgctggacg cggaaagacc gctcggcaac gggcatcttt tgccgcgcgg
     3181 cgagctgcgc gaaccggtcg aaagccttcg ccgggccgac tgtattgtgc tgacgcgcgc
     3241 cgacaaagac ggcccgacgc ccgcccggat cgcgcggatc gcggaagacc gcggcattcc
     3301 cgtcttcaga gccgttcacc gcttcaggga aataatcgga cccgacggcg gcgtcctgcc
     3361 gcccgaacat ctccggggca aaaaagtctg cgccttctgc ggcatcgcga agcccgcatc
     3421 ctttaaaaaa atcctgatcg aagcggaagc ggaggtcgtg tcctttattg actttcccga
     3481 tcattacgtt tacaaccgcg acgacctgga agcgctgggc tggcattttt cggcgcaaaa
     3541 cgccgattgc cggctgacca cggaaaaaga cgccatgcgc ctggaggcct ttccggattt
     3601 cttaaaaatg cttttcgtcg cgcgcgtgga aatggacatt cagccggcaa aacgatttga
     3661 agatttcatc atcaaccgcc tggccggagc ctcaacttca ggaaagacat gaaaaccaaa
     3721 ccgtccgcta aaatcagcat cacgattgcc gccgcgaaaa tgatcccgct tgcggtcagg
     3781 agatggatcg caaaccgcat cggcggcctc gggtattgcc tgtcgctgaa gcacaggctc
     3841 attgccctgc ataatctcat gcgctctttt ccggaaaagc ccctcgatga aatcgtcgcc
     3901 atcgccaggg cctcttacaa aagctttgcc ctggtttttg cggaatttcc ggaaattctt
     3961 tgtcttgaca gaaacaacat tagaaaatgg gtttccgtcc gcggactgga gcattacgaa
     4021 gcggctcttc gcgaaggaaa gggcgttgtg ctaattacgg cgcacttcgg caactgggag
     4081 atgggcatgg ccgccatggc ccttctgtcc cgcccgccga ccttcgtagc gcgtgtgctc
     4141 gacagcgcga tccttgaaga gggaagcgtt tatatccgct cgacggtggg aatcggcatc
     4201 attcacaagg aaaacgccat gcggcccatg cttcgtctgc tgaagcaggg cgaagccgtc
     4261 aagatgttga tcgaccagaa cgttgccgct tatgagggcg ttttcatcaa cttcttcggg
     4321 cggccggcct gtacaacttc aggagttgcg ttaatagcga tgcgctcagg ggctgccgtt
     4381 cttccggtct ttacaaaccg catgcccgac ggcacatatc tgaccgaaat cggtccgaaa
     4441 gtggaaaccg tccacacagg ggacagacgc cgcgatgtgg cgaccaatac gcaaaattac
     4501 gcgaccgcaa tcgagaatca catccgcaaa tatccagaac agtggctctg gctgcatcag
     4561 cgctggaaga ccaagacttg ccaggcgaag gaatagtggg tggcgtccct cgacaggctc
     4621 gggatgacaa ggttgtttgt catggtgagc tcgtcgaacc atgnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnaccgttc cgtacgggag ctggattccc gccccccgac
     4801 ttctcgaggg caggcttcgc gggaatgaag agaaaataga ttgtgcaaag tcaggtgatg
     4861 gggaaagatg ctggaagcaa aaaataaatt gccgctggag ggactgaaca aaatcctgat
     4921 ccgcggcacc aactggatcg gcgacgccgt gatgacgctg cccgccgtcg catccgtgcg
     4981 cgcggcctat cccggagcgc atctgtccgt tttagccaag ccgcccgtct ccgacatcta
     5041 caaaatgttt tccgccgccg acgaaattct tccctatccg cggaaattcg acaatgcgcc
     5101 gggcgtcctt cgcctggcgt attcgctccg gccgaaaaag ttcgatgcgg ccattttgct
     5161 gcaaaacgcc atcgaagccg ccatcatcgc ttatgtggcc ggcatcggcg tgcgggccgg
     5221 ctacagcacc gacggccgcg gctttctgct gacgcatccc gtccggcgaa gcgcggacat
     5281 cctccgcgtc catcagatcg actattatct ggaaatggtc aaggcgctgg gctgcgcgga
     5341 tgtagatcgc tcgctgcgcc tggaaacgag cctttcaacc gcaaccaccc gggaaattct
     5401 gcgcaaatac ctgccggaaa acaacaggcc cctcatcggc atcgctcccg gcgccatgta
     5461 cggcccggca aagcggtggc ttccggaaag attcgcgcag gcgggagaga gcctggcaaa
     5521 aaaattggac gctcaggttc ttcttttcgg aggcggggac gattgggaaa cggctgagct
     5581 ggtgcgcggg caggcgcaaa cggatatgat gaatctggcg ggccgcacat cgcttgagga
     5641 ctcggtgtat ctcatatcgc aatgccggct gtttctcagc aacgattcgg gcctcatgca
     5701 cgtggcggga ggcttgaata ttccgacggt cgcgattttc ggctccacca atccggttac
     5761 cacgtcgccc gcgggagaaa gaacgacgct ggtgcgcaaa gaaacgccct gcagcccctg
     5821 tttgaaaaaa acctgtccga cggacttccg ctgcatgacc gcggtcaccg tcgaagacgt
     5881 cgtcgaggcg gcgctgaccc tgatcaacgg aacaaccggt ccgcgccccg atcaccgaaa
     5941 gagttgacgc cggatgaaaa aaaacgcagc catctttctg gaccgggacg gaaccatcaa
     6001 cgaagaagtc ggctacctcg actctccgga caaactggta attttcccgt ccgccgttga
     6061 agcggtccgt ctgatcaacc aaagcggttt caaggcgatt gtcgtcacca accaggcggg
     6121 cgtcgcccgg ggcctgttcg gcgaagacct tgtggaaaaa attcacgccg ccctgcggga
     6181 cgctctgcgt caaaggggtg ctctcctcga cgcgttttac tactgtcccc atcatccaac
     6241 cgagggattg cctccctacc ggcaaagctg cgactgccgc aagccgtcgc ccggtctgtt
     6301 tttacaggcg gcgcgggagc tgaatcttga cttatccgca tcctggatga tcggtgaccg
     6361 atacaatgat atcgaggcgg cccaccgggc cggcgcgaaa ggcatcctcg tcaggacggg
     6421 ctacggggcg gacgagcttt ccggcgaggg gcccgacgcc gagacgccgg cgggaaagcc
     6481 ggagttcatc gcggacaac
//
LOCUS       DAOSRM010000105         6469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_11016_length_6469_cov_88.143748, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000105 DAOSRM010000000
VERSION     DAOSRM010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11016_length_6469_cov_88.143748"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..1400
                     /locus_tag="PLA58_09055"
     CDS             402..1400
                     /locus_tag="PLA58_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HOR62980.1"
                     /translation="MAGITYKGLMVREIAENQFARAVEELSTDQLPAGDVLVKVLFSS
                     LNYKDMLSADGNRGVTKKYPHTPGIDAAGIVEESSSPDFKAGDEVIVTSYDLGMNTSG
                     GLGQYIRVPSAWVVRKPASLSFRESMIFGTAGFTAALSILRLVEFGMDPADEILVTGA
                     SGGVGSIAVSILSHAGYNVTALNSLQDESAFLKKIGAKQVIEPAELVDASGRPLLKER
                     WTKCVDTLGGDVLSSAVRSIKGYGAITCCGNVISPDLKLTVFPFILRGVTLYGIDSQN
                     CPMPRRQKAWDMIADRWKFPWLATLSSEVPLGGVTGVMDEIKAGRHRGRTVVNLWE"
     gene            complement(1491..2930)
                     /locus_tag="PLA58_09060"
     CDS             complement(1491..2930)
                     /locus_tag="PLA58_09060"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HOR62981.1"
                     /translation="MIKTRQDYIDSLKRQHLNVYYNGERVEDITAHPGFIPHINAAAK
                     TYELALLPESEDLLTAISHLTGKKISRFTHIHQSVDDLIKKVQMLRLISHETGSCYQR
                     CVGFDALNATYMTTYDIDAVYGTNYFSRFCDFLRYVQEENLMVVGGMTDPKGDRSKRP
                     SDQSDPDLFTHVTEKRSDGIVIRGAKAHMTGGVNSHEILIMPTQNMTKGDEAYAVCAA
                     IPLKSPGITLIFGRQSNEERKFETGIDAGNKDYGVVGGEALIVFEDVFVPWERVFMCG
                     EIDFTGLLVERFATMHRQNYGGCKGGVSDVIVGATALAAQYQGTSGASHVKDKITEMM
                     HLAESVYSGSVACSAMGSRTPSGAYYPDPLLANCTKHNITRHIYEISRLAHDVAGGIM
                     ATMPFDRDLRSPEIGKYVEKYLAGVNGVPAETRMKVLRLIENMTGGTCLVESMHGAGP
                     PQSQRVMYQRLGNLPQKIKMAKKLAKIDE"
     gene            complement(2927..3244)
                     /locus_tag="PLA58_09065"
     CDS             complement(2927..3244)
                     /locus_tag="PLA58_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62982.1"
                     /translation="MGCYLIAWNMKTEGKTVIVKFCGGCNPKYDRVAYWEEIKTRAGD
                     AVTWVAADFPRPDGMLLICGCHSVCPLKHYNQADYGFFILVDSDRKSPGSVAGSIIDK
                     EGT"
     gene            complement(3257..3526)
                     /locus_tag="PLA58_09070"
     CDS             complement(3257..3526)
                     /locus_tag="PLA58_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62983.1"
                     /translation="MGLIARALEEKGIPTVSLSSAYDLTTLVKPPRTFFVNYPLGHTS
                     GKPFDRENQTAILKDALGGAREIATPGAIVSLPYEWGDPFWLAGA"
     gene            complement(3545..4021)
                     /locus_tag="PLA58_09075"
     CDS             complement(3545..4021)
                     /locus_tag="PLA58_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HOR62984.1"
                     /translation="MKYLKYVDVVQSMFPDQPPYQWTVNTSVPWTPIVLPLAESTVSM
                     ISSSGVYRKDQTPFKPDKNDLSFREIPADTDAGDLKISHDYYDHRDAEKDVNCVFPIE
                     RLRELAAEGFIGGLSPFHLTFMGRVFRKTQFLDEMIPKMLARIEAMKTDLVLLVPA"
     assembly_gap    4152..4251
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4312..5013
                     /locus_tag="PLA58_09080"
     CDS             4312..5013
                     /locus_tag="PLA58_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOR62985.1"
                     /translation="MPRTARIAPKEFVYHTLTRGNNKQDIFKDEEDFKQYIDILLKYK
                     SKYNYKLYHYVLMTNHVHLVIEPTENGGELSQIMKGINLSYAQYFKKKFKHTGHFWQD
                     RFKSIIISKDEYLLACGSYVELNPVRAGMVKDPKDYPWSSYKANAYGEKNDLLDQHIF
                     YDKLATEETVRKEYRSFVQQMLKKNESMRGEMNRRVIYGGGDFTQRVNKRYKVDAVIK
                     KRGRPKKESADKPIK"
     gene            5059..5961
                     /locus_tag="PLA58_09085"
     CDS             5059..5961
                     /locus_tag="PLA58_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR62986.1"
                     /translation="MTQKKKALCITWLGTAGFLIESGHDGILIDPYVSRAGMLRVFLG
                     LPLRPDQPAIDKWLVRLETKIHAVIVSHSHFDHCLDAPYFALKTNALLMGSKSTLNVG
                     RGVGLDEKYLKEAEIGRDIHIGPFTLKFIESAHGPAFLGKVPYPGKIDQPLTGPHPAR
                     DYKLGETYSILIRHPSGAILHHGSAGFINGMYEGITSDMALLGIGGRENTQTYLENVA
                     LKLNAKIVVPIHYDNFFVSLDKKMKYLPGVNFREFLATAGKYRNVFELKVLSIGEKWS
                     CNSLISDELVKSNPNRHPGESRGP"
     gene            complement(6054..>6469)
                     /locus_tag="PLA58_09090"
     CDS             complement(6054..>6469)
                     /locus_tag="PLA58_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR62987.1"
                     /translation="SEAEGLVVHVLNSRQVEAMCCCCSCCCGMLISLKLFPAPALAVK
                     SNYVCRFDEALCSQCGLCAGRCTVDALGMKDEKIVFDAGRCIGCGLCVTVCPAGALHL
                     DKKPEDSLYQPPETVFDAFAAMSKEKAAKKGDGSI"
BASE COUNT         1540 a         1727 c         1703 g         1399 t
ORIGIN      
        1 taaaatatga ggttatatag atttatggcg ggcgcaatcg aagaacgatt gagtcaacgg
       61 cacggtagta gaatcaaaca ggtattcaat ctctcgtcca tcctcgaatt ttcggttgaa
      121 gaaaagctta aagagtggaa taatcgcagc cgttacggag agtttaaact ttgtcatttc
      181 gaggagcgtt ggcgacgaga aatcttaacg cttgtgaaga gggatagatt tctcccttcg
      241 gtcgaaatga ccaaaacggc agcgcttcaa agctctcgtc atggtcaggc ggttttaccg
      301 ggccggaccg atgagtttta tttccttaaa tcgaagcatg agatacatat ccaggttcat
      361 ggatatgaac gaccctttca atccattccg aggaggttag tatggcaggc ataacgtaca
      421 aagggctgat ggtcagagag atcgccgaaa accagtttgc aagagccgtt gaagaattat
      481 ccaccgacca actcccggcg ggagacgttc tggtcaaggt gctgttttca tcgctgaatt
      541 acaaggatat gctttccgcc gacggcaacc ggggcgtgac gaaaaaatat ccccacaccc
      601 ccggcatcga tgcggcgggc atcgtcgaag aaagcagttc gcccgacttc aaagccggcg
      661 acgaagtcat cgtcaccagc tacgacctgg gcatgaacac atccggcggt ctgggccagt
      721 acatccgggt tccgtcggcc tgggtggtca gaaagccggc aagcctgtcg tttcgcgaaa
      781 gcatgatttt cgggacggcg ggattcacgg ccgcgctttc gattctgcgc ctggtggaat
      841 tcgggatgga cccggccgat gagattctgg tcacgggcgc aagcggcggc gtgggcagca
      901 ttgcggtttc cattctttct catgccggtt acaacgtgac ggcgctcaac agccttcagg
      961 acgaaagcgc gtttctgaag aaaatcggcg ccaagcaggt catcgagccc gccgagctgg
     1021 tcgatgccag cggacggccg ctgctgaaag agcgctggac caaatgcgtc gatacactgg
     1081 gcggcgatgt cctttcgtcg gctgtccggt ccatcaaagg atacggcgcc atcacctgct
     1141 gcggcaacgt catttcgccg gatctcaagc tgacggtctt tccgttcatt ctgcgcggcg
     1201 tgacgctcta cggcatcgac tcgcagaact gccccatgcc ccggcgtcag aaggcctggg
     1261 acatgatcgc cgaccggtgg aaattcccct ggctcgcaac gctgtcttcg gaagtccccc
     1321 tgggcggcgt aaccggcgtc atggatgaaa tcaaagccgg acgccatcgc ggcagaaccg
     1381 tggtcaatct gtgggagtga ctggataaaa aataccctgc tgataatact gaacaccggg
     1441 aggaatatgg ggggcatgaa tggccgcccc cctgcctgaa agatttctgc ctattcatca
     1501 attttggcca gttttttggc cattttgatt ttctggggaa gattccccaa ccgctggtac
     1561 atcacccgct ggctttgcgg cgggccggcg ccgtgcatgc tctccacgag acaggttcca
     1621 cccgtcatgt tttcaatgag acgcaaaacc ttcatccttg tctcggccgg aacgccgttc
     1681 acgccggcca ggtatttctc cacatacttg ccgatctccg gactccgcag gtcccggtca
     1741 aaaggcatgg ttgccatgat gcccccggcc acatcatgcg ccaggcggga aatctcgtaa
     1801 atgtgacgcg tgatgttgtg cttcgtacag ttggccagaa gaggatcagg ataataggcg
     1861 ccgctggggg ttctggatcc catggccgaa caggccacgg aacccgaata caccgattcg
     1921 gccagatgca tcatctccgt gatcttgtcc tttacatggg aggcgccgga cgtcccctgg
     1981 tactgggccg ccagagccgt agccccgacg atgacatccg ataccccgcc cttgcaaccc
     2041 ccgtagttct ggcgatgcat cgtggcgaac ctctccacca atagacccgt aaaatcaatc
     2101 tctccgcaca tgaatacccg ctcccacgga acgaagacat cctcgaaaac aatgagggcc
     2161 tctccaccga ccacaccgta atccttgttc cctgcgtcga tccccgtctc gaacttccgc
     2221 tcctcgttcg attggcggcc gaaaatcaga gtgatccccg gcgatttcag gggaatggcc
     2281 gcacacaccg catacgcctc gtctcctttg gtcatgttct gcgtgggcat gatgagaatc
     2341 tcgtggctgt ttactcctcc cgtcatatgc gccttggctc cccgaatcac aatcccgtcg
     2401 gaccgcttct ccgtcacatg cgtgaagaga tccggatcac tctgatcgct gggacgcttg
     2461 gaccggtccc ctttgggatc cgtcatcccc cccaccacca tcaggttctc ctcctgaacg
     2521 tatcgaagaa aatcgcaaaa acgggaaaag taattcgtcc cataaaccgc atcgatgtcg
     2581 tacgtggtca tgtacgtcgc attcagggcg tcgaatccca cgcaccgctg gtagcagctt
     2641 cccgtctcat gggaaatgag acgcagcatc tggactttct tgatgagatc gtccacggac
     2701 tgatggatgt gcgtgaaacg gctgatcttc ttgcccgtca aatgacttat ggccgtcaaa
     2761 agatcctccg attccggcag aagggccaac tcatatgtct tggccgcggc gttgatgtga
     2821 ggaatgaaac ccgggtgtgc cgtgatatcc tccacacgct ccccattgta atacacgttc
     2881 aggtgctggc gcttcaaact gtcgatgtaa tcctgcctgg tcttgatcat gttccctcct
     2941 tgtcgatgat actaccggca acagacccgg gactcttccg gtcgctgtcc accaggataa
     3001 aaaaaccata atcggcctgg ttgtaatgtt tcaaggggca gacgctgtga cagccgcaaa
     3061 tgaggagcat cccgtccggt cgggggaaat cggcagctac ccaggttacg gcgtcacccg
     3121 cccgagtctt gatttcctcc cagtaggcca cgcggtcgta tttggggtta cagcctccac
     3181 agaacttgac gatcacggtc ttcccttcag tcttcatatt ccatgcaatg agatagcagc
     3241 ccatggccgc ttcctgtcac gccccggcaa gccagaacgg atcgccccac tcgtagggaa
     3301 gcgagacgat ggcgccgggc gtggcgatct cgcgggcgcc gcccagagca tccttcagga
     3361 tcgctgtctg gttctcccgg tcaaacggct tgcccgaggt atgaccgagc gggtagttga
     3421 cgaagaaggt ccgcggcggc ttgacgagcg tcgtcaggtc gtaggccgag gacagagata
     3481 cggtcgggat gcctttctct tcaagggccc gggcaatcaa tcccacggat tgatggcaga
     3541 gcggtcaggc aggcaccagc aagacgagat cggtcttcat cgcctcgata cgggcgagca
     3601 ttttcgggat catctcgtcg aggaactggg ttttccggaa aacgcggccc atgaaggtca
     3661 ggtggaacgg cgaaagcccg ccgatgaacc cctcggcggc gagctcccgc agccgctcga
     3721 tcgggaagac gcagttgacg tccttctccg cgtcccggtg gtcgtagtag tcgtggctga
     3781 tcttgaggtc gcccgcatcc gtgtccgcgg gaatctcacg gaaagacagg tcgttcttgt
     3841 cgggtttgaa cggggtctga tctttgcggt acacgccgct ggaactgatc atcgagacgg
     3901 tggactccgc caggggcagg acgatgggcg tccaggggac ggaggtgttg accgtccact
     3961 ggtaaggggg ttgatccgga aacatggact gtacgacgtc aacgtacttg aggtacttca
     4021 tcgctctctc cttccggtcg gtatgggcat gtgtgcagaa tctagccgat atcccgggaa
     4081 aatgtcaata agaagtcagg gagcgagaaa aggggacggt tctatttatt gacagcgatt
     4141 tttggttttg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgagaaaag
     4261 gggacggttc tatttattga cagcgatttt tggttttgat atagaaacac catgccaaga
     4321 acagcaagaa tagccccgaa agaattcgtt tatcatactc tgactcgcgg aaataataag
     4381 caggatattt tcaaagacga agaagatttt aaacaataca ttgatattct cctgaagtac
     4441 aaatcaaaat ataactacaa gctataccac tatgttttga tgaccaacca tgtccatttg
     4501 gtcattgagc ccacggaaaa cggcggcgaa ctttcccaga tcatgaaggg gatcaatctg
     4561 tcctatgctc aatattttaa gaagaagttc aaacacacgg gccacttctg gcaggaccga
     4621 tttaaaagta tcatcatttc caaggacgaa tatctgctgg cctgtggaag ctatgtggaa
     4681 ttaaatcccg tccgggctgg aatggtgaaa gacccaaaag actacccttg gagcagttat
     4741 aaggccaatg cttacgggga gaaaaacgat ctgctcgatc agcatatttt ctatgacaag
     4801 ctcgcgactg aagagaccgt aagaaaggaa taccgttcat ttgttcagca gatgctgaag
     4861 aagaatgaat cgatgcgggg cgagatgaat agaagagtaa tctatggagg gggcgatttt
     4921 acacaacggg tcaacaagcg atacaaggtt gatgccgtga tcaagaaacg gggacggccg
     4981 aagaaagaat cagccgacaa accaattaaa tagaaccgtc ccctttttcc acccttttca
     5041 ggtcagaaag gacaaagcgt gacgcagaaa aaaaaagcct tgtgcattac ctggctgggc
     5101 acggcaggtt ttctgattga gtctggtcat gacggtattt tgattgaccc ctacgtcagc
     5161 cgtgcaggga tgttgagagt gtttctgggc cttccgctga gacctgatca gccagccatc
     5221 gacaaatggc tggtccgtct ggaaacaaaa attcacgccg tcattgtcag tcattcccac
     5281 ttcgaccact gccttgacgc tccttatttt gccctcaaaa caaacgccct gctcatgggt
     5341 tcaaaaagca cgctgaatgt gggacgcggc gtcgggctgg atgaaaagta cctgaaagag
     5401 gcagaaatcg gccgggatat ccacatcgga cccttcacgc tcaaatttat tgaaagcgct
     5461 cacggaccgg cttttctcgg aaaagtcccc taccccggaa aaatcgacca gccgctgacc
     5521 ggcccgcatc ccgcaagaga ctacaaactg ggagaaacgt attccattct gatcagacat
     5581 ccttccggcg ccattctgca tcacggcagc gccggattta tcaacggaat gtacgaaggc
     5641 atcacatctg atatggcgct gctgggcatc ggcggcaggg aaaatacgca aacctatctt
     5701 gaaaacgtag cgctgaaact aaacgcaaaa atcgttgtcc cgatccacta cgataatttt
     5761 tttgtttccc tggataaaaa aatgaaatat cttcccgggg taaacttccg ggagtttctg
     5821 gccacggccg gtaaataccg aaatgttttc gagctgaagg tcctgtccat cggtgaaaaa
     5881 tggtcatgta attccttgat atctgatgag ctcgtaaaaa gtaacccaaa ccgtcacccc
     5941 ggcgaaagcc ggggtccata actaattgta attactggat accggtcttc gccggtaaga
     6001 cgaaaagagt gcgaaattga ctttttacga gaccgtcata tcaagcccgc cgattaaata
     6061 gaaccgtccc cttttttagc ggctttttct ttgctcatgg cggcaaaggc atcaaaaacg
     6121 gtctccggcg gttgatacag actgtcctcc ggtttcttgt ccagatgcag cgcgccggcg
     6181 ggacaaaccg taacgcaaag cccgcagccg atgcagcggc cggcatcaaa aacgattttt
     6241 tcatctttca ttccgagagc atccacggtg cagcggcccg cgcaaagacc gcactgcgag
     6301 cagagggctt cgtcaaaacg gcacacgtag ttgcttttca cggccagggc gggcgccggg
     6361 aaaagtttga gcgaaatcag catgccgcag cagcaggaac agcagcagca catcgcttcc
     6421 acctgccggg aattcagaac gtggaccacc aacccttccg cctcgctct
//
LOCUS       DAOSRM010000106         6399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_11153_length_6399_cov_99.526639, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000106 DAOSRM010000000
VERSION     DAOSRM010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11153_length_6399_cov_99.526639"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLA58_09095"
     CDS             complement(<1..224)
                     /locus_tag="PLA58_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62988.1"
                     /translation="MLSRAHADIHVNLVEEAQPLADRSETAVLLEEGVRQFEDLFCAR
                     PAAASVDLVNSLFDELVKSNPHRHPGESRGP"
     gene            920..1657
                     /locus_tag="PLA58_09100"
     CDS             920..1657
                     /locus_tag="PLA58_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOR62989.1"
                     /translation="MKDILKKIDSIKTKIDAHRPLDSHMVRELREYFRIGMTYSSNAL
                     EGNSLTETETKIVIEDGITIGGKPVKDHLEALGHSEAYDLLFRLAKHQDISEANIKEL
                     HRLFYYRIDEDQAGKYRKQKVIITGTTFIPPAPEQIPDLMKSFVARIPDDRSKHHPVE
                     YAAILHRKLVTVHPFIDGNGRAARLLMNLALLQAGYPVAIIPPVLRRDYLDTLNKTHK
                     GDDKAFNKFIAGVCYESSKEYLRLLEG"
     gene            complement(1995..2071)
                     /locus_tag="PLA58_09105"
     tRNA            complement(1995..2071)
                     /locus_tag="PLA58_09105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2035..2037),aa:Met,seq:cat)
     gene            complement(2276..3133)
                     /gene="speB"
                     /locus_tag="PLA58_09110"
     CDS             complement(2276..3133)
                     /gene="speB"
                     /locus_tag="PLA58_09110"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOR62990.1"
                     /translation="MNFGGIYPECSLGNARFVVVPVPYDLTSTYQTGSRRGPAAIIEA
                     STNMELYDEELKKETYLAGIHTAEPVAVDARGPKTMIAAVRKKISRIVAQDKIPVMLG
                     GEHSITLGAVQAVAEKYPKLTVLQFDAHADLRDSYQGTPYNHACVARQIAGFCDLVQV
                     GIRSMSREEGDFLHTSKVKSYPADDVFDHANWAEKVAGQIKGDVYITIDLDVFDPSVM
                     PATGTPEPGGLFWRDVLSLLRLVSRKCIVRGFDVVELAPLPGIVAPDFMAAKLIYRLM
                     GYITESRKK"
     gene            complement(3217..3999)
                     /locus_tag="PLA58_09115"
     CDS             complement(3217..3999)
                     /locus_tag="PLA58_09115"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOR62991.1"
                     /translation="MKVVFLSDAHLKRSTDERYGLLVRFLTELGEGRIRSLAGDEKTV
                     RVNAAIDDLYILGDFFDFWFCRPDRIYPEFQPVIRRLVQLRQSGVRIHLFEGNHDFFM
                     REYFHDVLGMNVFEESASIDLDSSRVFVAHGDTLDRTNVFYLTLRKVLRSRIFYHFQR
                     LMPASALWAIAALSSATSKTVTVEDGDVLVEKLYACAEEKLFREYDTVILGHCHKPVL
                     RSSDREGRRKTFVALGDWIVHKSFLYYEDGKFFMEYYPAEPG"
     gene            4163..5170
                     /locus_tag="PLA58_09120"
     CDS             4163..5170
                     /locus_tag="PLA58_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOR62992.1"
                     /translation="MQNKNMSSSRTAVSRAREVLKIEAQSILSLVHKINGNFSRAVDL
                     IFQCKGRVIITGIGKSGLIGRKIVATLTSTGTQALFLHPVEGIHGDLGIVTKKDILLA
                     ISNSGETRELLPIISSVRKIGAPIISFTGVLKSTLAQNSDIVIDVSVEKEACPFGLAP
                     TSSSTAALAMGDALAIALIDKRKFREKDFYKFHPGGSLGARLRATVRDAMITGDRIPR
                     VITGTPARQAIEVIDRMNVGFVLVTDKKNHLIGILTDGDVRRMVSRGSSFDGLTIDRV
                     MTANPKTIDEKASLAETVEFMQKKEITSLAVVNEKKALKGYVHLHDIFGRGGSVNISL
                     A"
     gene            5400..5933
                     /locus_tag="PLA58_09125"
     CDS             5400..5933
                     /locus_tag="PLA58_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOR62993.1"
                     /translation="MALYEFEGKKPRVPSDSFVHPTAVLIGDVRLGHECLIAPGVSIR
                     ADFGPVIIGDRSSVQDNSVIHVYPGSESVIGNDVTVAHGVILHDVHIHDRCMIGIGAI
                     ILFNVVCEEDVFVSVGSMVPRGMRVPAGKIVAGNPAKITRDVTDKDKAAAKDGIEAYR
                     YLCRQYLATMKTISESL"
BASE COUNT         1705 a         1677 c         1582 g         1435 t
ORIGIN      
        1 ggaccccggc tttcgccggg gtgacggtgt gggttgcttt ttacgagttc atcaaataag
       61 gagttgacca gatcaaccga agcggcggca ggcctcgcgc aaaaaaggtc ttcaaactga
      121 cgtacgcctt cttccagcag aacagcggtt tccgacctat ccgcgagggg ctgcgcctcc
      181 tccaccagat taacgtgaat gtccgcgtgc gcccgggaca gcatctgttg ggtttttgct
      241 cagccgcccg gacggaaaat accataccga ccagaatcag aaccacaaca aaccactgcg
      301 acgcttgtat cggatattgc ttttgccgcg caaaccataa aaaactcggt ttaggatgtc
      361 aagaaaatcc tgagcgcggc taagcaaggg atacagcgca aagttggcat ccgatattct
      421 tgcggaaaga atacgcgcaa gaaagaaatg accggcgcgg ggttgtcccc gaagactatt
      481 acccattgct tgtcgttaat tcgggccatg tataactttt gggcaagccg gggaccgtac
      541 aacggggaaa accccattaa gaaaaagagc ggtcaaccga gccggaatga aattgagcaa
      601 tcttgaccct tcccatattt tggtataggt agaaacatga attggatggc aacactgaac
      661 cggaaggaaa gacaaattga ggtgatttat gttggggccc acgaaggcgc gccataccaa
      721 aaacattaaa cttcatctga taggccccgc gcatagaaaa gacaaggcga tggaaatcct
      781 taaaggcatt taagcgaaat ggaacacggc aagcgcacca ttggcaagaa aaacgcaaag
      841 ctctttgccg aattattgaa cacggattac cgagtttttc tctaaccgga tgcggcatgc
      901 ttttgaggaa ggaaacggga tgaaagacat actcaagaaa attgattcga tcaaaacaaa
      961 gattgacgca cacaggccgt tggattcgca catggtcaga gagctacggg aatatttccg
     1021 aatcggcatg acctattcaa gcaatgctct ggaaggaaac tctttgaccg aaacggaaac
     1081 gaaaatcgtc attgaagacg gaatcaccat tggcggtaag ccggtcaaag atcatctgga
     1141 agctttggga cattcagaag cctatgattt gcttttccgg ctggctaaac atcaagacat
     1201 ttcagaagcg aatatcaaag aactgcatcg gctgttttat tatcggattg atgaagatca
     1261 ggccggaaaa taccggaaac agaaagttat cattaccggc acaaccttca ttccccccgc
     1321 tcctgaacag ataccggatt tgatgaagtc atttgttgcc aggatacccg atgaccggtc
     1381 aaagcatcat cccgttgaat acgcggcaat cctgcatcga aagcttgtaa ccgttcatcc
     1441 gttcattgac ggaaacggaa gggcggcgcg cctcttgatg aaccttgcgc tattgcaggc
     1501 aggctatcct gtggcaatta ttcccccggt cctgcggcgc gactatctgg acactttgaa
     1561 caaaacccat aagggcgatg acaaggcgtt caacaaattt attgccggcg tctgctacga
     1621 atcatcaaaa gaatatttaa ggcttctgga aggataagtt ttcataatcg ggaataggca
     1681 ggccagtcga caagcgggag aaccccttcc cgcttagcat aggggatctt gtgagaggga
     1741 aagcatggag caaggcagtc atgacttgat gaaacaaagc gtcaggttaa ggctggataa
     1801 tataactcag ttaggtgtaa tcaataaacc aacaaaaata aatcggaaat ccaaaataaa
     1861 accttttacc gaatggactg aacaagaacg tcgaacgcgg gtttatgtga tgagatactt
     1921 taaaaagtgg tagcatcgtg gtagcgcaga aaaaaagagg gtttagaaaa actctctaaa
     1981 cccttgattt tatttggtag cgggggatgg atttgaacca tcgacctttg ggttatgagc
     2041 ccaacgagct accaagctgc tccaccccgc gataagtcct ttgtaagttg gtggctttaa
     2101 aacattatct cctgtgtttg tcaagcggtt atttggccac aaaggccgcg tgccgcttgc
     2161 ataaagactc cggctgcaat gaacgccatc ccccggttga ttcagaacgc cgcctttcca
     2221 tccattgtcc ctccgatacc aaacggatca atccccgcag cgcgtcgaag caaaattatt
     2281 tttttcggct ttccgtaata taccccatca accgatagat cagctttgcc gccatgaaat
     2341 cgggcgcgac gatgccgggc agcggagcca gttccaccac gtcaaacccg cgcacgatgc
     2401 atttgcggga caccagccga agcagactga gcacatcgcg ccagaaaagg ccgcccggct
     2461 cgggtgttcc ggtagcgggc atgaccgaag gatcaaaaac atccagatcg attgtaatat
     2521 aaacgtcacc cttgatttgc ccggcaactt tttcggccca gtttgcatga tcgaaaacat
     2581 catccgccgg ataggatttg accttgctcg tgtgaaggaa gtccccttct tccctgctca
     2641 tgctgcggat gccgacctga accagatcgc aaaatccggc aatctgcctg gccacgcagg
     2701 catgattata aggcgtgccc tgatagctgt cgcgcaaatc cgcatgtgcg tcaaattgca
     2761 gaaccgtcaa cttcggatat ttctccgcga ctgcctgcac agctcccagc gtaatgctgt
     2821 gctcgccgcc cagcataacc ggaatcttgt cctgcgcgac gatccggctg atttttttgc
     2881 gcacggcggc aatcatcgtt ttcggtccgc gggcgtcaac ggcaaccggt tcggcggtat
     2941 ggattccggc cagatacgtt tctttcttca gttcctcatc gtagagctcc atattggttg
     3001 acgcttcaat gatggctgcc ggtccgcggc ggctgccggt ctgataggtc gaggtcagat
     3061 catagggaac gggaacgacg acaaaccgag cgttgccgag tgaacattcg ggatatattc
     3121 cgccaaaatt catgatgttg tccctctttt tctgaaaaag tgaggcttcc tagcacgaag
     3181 aagcggagat gtccagtttt tcaccgaaca ccgccttcac cccggttcag ccgggtaata
     3241 ctccatgaag aatttgccgt cttcgtagta caaaaatgat ttgtggacga tccagtcgcc
     3301 aagcgccaca aaggttttcc ttcggccttc cctgtccgaa gatcgcagca cgggcttgtg
     3361 gcaatgccct aggatcaccg tatcatattc ccggaacagc ttttcttcag cgcaggcata
     3421 caatttttca accaggacat ccccgtcttc aacggttacg gttttgctgg tcgctgaact
     3481 taaggccgca atcgcccata aggcggatgc gggcatcaag cgctgaaaat gataaaaaat
     3541 ccggcttcgc aaaactttcc gcagcgtgag ataaaacaca ttggtccggt ccagcgtgtc
     3601 accgtgggcc acaaaaacac gactggaatc cagatcaatc gatgccgatt cctcaaagac
     3661 gttcattccc agcacatcgt gaaaatattc ccgcatgaaa aaatcatggt tcccctcaaa
     3721 caaatgaatg cggacgccgc tttgtcgcaa ttgaaccagt cggcggatga ccggctggaa
     3781 ttccggataa atgcggtcgg gacggcaaaa ccaaaaatca aaaaaatcgc caagaatata
     3841 caaatcatcg atggccgcgt ttacacgaac cgttttttcg tcgccggcca gtgacctgat
     3901 cctgccttcc ccaagctccg tcaggaaacg gaccagcagg ccgtatcgct cgtctgtcga
     3961 acgcttcaaa tgggcatcgg atagaaaaac aactttcatt gtcaaattct caaaaaatct
     4021 ccaatcggcc ttcttgtgcg caaccatgat ggattttaca gcgtctgtca aataaaattc
     4081 gtgagtaatt gattcttgtt ttgtccggtg ttttttgcta actttcccaa aacgtggatt
     4141 tacgggccta aaaggaaata ccgtgcagaa caaaaacatg agcagcagcc gaaccgccgt
     4201 ttcacgcgcc agagaagttc tgaaaatcga agcgcagagc attttgagtc tggttcataa
     4261 gattaacggg aacttttccc gcgccgtcga tttgatcttt cagtgcaagg ggcgcgtcat
     4321 catcacgggc atcggcaaat ccggcctgat cgggcggaaa atcgtcgcca cgctgaccag
     4381 cacgggcaca caggcgcttt tcctccatcc ggtggaaggc attcacggcg acctgggcat
     4441 cgtcaccaaa aaagacattc tgctggccat ttccaacagc ggcgaaacgc gggagctgct
     4501 gccgatcatc agctctgtca gaaaaatcgg cgcgccgatc atctccttca ccggcgttct
     4561 caaatccacc ctggcccaaa acagcgacat cgtcattgac gtgtccgtgg aaaaggaagc
     4621 ctgccctttc ggcctggcgc cgacctccag ctccaccgcc gcgctggcca tgggagacgc
     4681 gctggccatc gcgctcatcg acaaaaggaa attcagggaa aaggattttt acaagttcca
     4741 ccccggcggt tccctgggcg caagacttcg ggcaacggtc cgggacgcca tgatcacggg
     4801 ggacagaatc ccgcgtgtga tcaccggaac accggcccgg caggcgatcg aagtgatcga
     4861 ccggatgaat gtcggattcg tgctggtgac ggataaaaag aaccacctga tcggaattct
     4921 gaccgacggc gacgtacgcc ggatggtcag ccggggttcg tcctttgacg ggcttacgat
     4981 tgaccgggtc atgacggcaa atcccaaaac catcgatgaa aaagcgtccc tggcggaaac
     5041 cgtcgagttc atgcagaaaa aagaaatcac ctctctggcc gtggtcaatg aaaagaaagc
     5101 attaaaaggc tacgtccacc tgcacgatat tttcggccgg ggcggctcgg tcaatatatc
     5161 cctggcctga acggttttaa gtaaagcatg ccggccggtc ccacaaaaac agccggccgg
     5221 tttatttaag ggagaacttt gcatcattac acttttgtca tcccgaggag cgaagcgacg
     5281 tggcgatctc ttccaatatc aaacgcttat gagatcgcct cactttgttc gcaatgactt
     5341 aagagacatt atgcaaaggg ttctaacgga ttgctgcttt ttgatgagga tgtcttttta
     5401 tggccctgta cgaatttgaa gggaaaaaac cacgggttcc gtccgattct tttgttcacc
     5461 ccacggccgt tttaatcggc gacgtccgcc tcgggcacga atgtctgatc gcgccgggcg
     5521 tttccatccg tgcggacttc ggcccggtca tcatcggcga ccgatccagc gtgcaggaca
     5581 attccgtcat tcatgtctat cccggttccg agtccgtcat cggcaatgac gtcaccgtgg
     5641 cgcacggcgt catcctgcat gacgtccaca tccacgatcg atgcatgatc ggcatcggcg
     5701 ccattatcct cttcaatgtt gtttgcgagg aagacgtttt cgtctccgtg ggatcgatgg
     5761 ttccacgggg catgcgcgtt cccgccggca aaatcgtcgc cggcaatccc gccaaaatca
     5821 cgcgggatgt gacggacaaa gacaaagcgg ccgcgaaaga cggcatcgaa gcctaccgtt
     5881 acctctgccg gcaatacctg gccaccatga aaaccatctc ggaatcgcta taagagattc
     5941 tttttcctgg ccttgatcag caaaacggtt gacaacaagc ggggcatcag cctcgcatcc
     6001 agccaggcca aaaccgcgta gaaccggttc attacctgag agctttgaat aacggccatt
     6061 tttgtcattt cgaccgaagg gagaaatctt caacaaacgg ccatccttaa gatttctcgt
     6121 ccctcacgct cctcgaaatg acatttttca aagctctcta cctgaaagag acgaatcaga
     6181 aactccggag aagcacacat cggttttttg ttttttgtct caataaccga cggtctcgta
     6241 aaaagtaatt caaaccgtca ctccggcgaa agccggagtc cagaacttat tgtaattact
     6301 ggataccggc ctttgccggt atgacgaaaa gggtgcgaaa ttggcttttt acgagttcat
     6361 caataaccag ttgtctaaga aaaaaggttc gaacgaaac
//
LOCUS       DAOSRM010000107         6331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_11327_length_6331_cov_83.299873, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000107 DAOSRM010000000
VERSION     DAOSRM010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11327_length_6331_cov_83.299873"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLA58_09130"
     CDS             <1..242
                     /locus_tag="PLA58_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOR62994.1"
                     /translation="TVKCQRERSQSLNRHLARRLLLDKIERQQKGFAEEEKAKLAKLR
                     RQKRKRSRRAKEKILAAKHQQAEKKGLRGKVIIDE"
     gene            complement(370..750)
                     /locus_tag="PLA58_09135"
     CDS             complement(370..750)
                     /locus_tag="PLA58_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOR62995.1"
                     /translation="MKKSLVYSVLAIVLVIVSAPFVLAAPEAAAAAPAGPVDYTKAIV
                     VGCSLLAAGIAIAFGTIGTGNGMGQGLNGATNAVGRNPEAQGKILLTMMVGLAMIESL
                     AIYALVIALIVLYANPLVKVVIGG"
     gene            969..1433
                     /locus_tag="PLA58_09140"
     CDS             969..1433
                     /locus_tag="PLA58_09140"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR62996.1"
                     /translation="MNRFSTRQLKAAKILQSDIPLSPRPFADIARASEMTSEELIAWI
                     RNLSREGIIRKVTTILRHQKAGYGKNVLVLWSVTPEAVEDAGRKLAGLPYISHCYERK
                     PFAGRYNLFTMLHAKAGDISHLLEEMSRLISCPDFLLLESLEEYKKTSPEYF"
     gene            1433..>1953
                     /locus_tag="PLA58_09145"
     CDS             1433..>1953
                     /locus_tag="PLA58_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR62997.1"
                     /translation="MSKNNIHPFTEAARVIAGGVNSPVRAWKSVGGDPLFVQRAAGSK
                     IYDLDGKEYIDYVLSWGPMILGHAHPDVIAAVCDAARRGTSFGAPTTAETEMALLIRE
                     AIPSMERVRMTSSGTEAAMTAIRLARGCTGRNMIIKFDGCYHGHADSLLIKAGSGVTT
                     LGIPGSPGIPEAVA"
     assembly_gap    1954..2053
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2113..3360
                     /locus_tag="PLA58_09150"
     CDS             2113..3360
                     /locus_tag="PLA58_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOR62998.1"
                     /translation="MGLPLAAHLSRHFKVIGFDISEANVAELKKGHDRTMELSDAELK
                     KARISFTSRPNDLASCRLFIVAVPTPVDHFHVPDLSAMENASALIGKHMSAGSCVVYE
                     STVYPGVTEDVCVPVLERASGLKFGRDFTVGYSPERINPGDKIHTVSNTIKIISASDS
                     ATLDLLEGIYSAVVKVRLHRVSSIKVAEAAKVIENTQRDINIALMNELAIIFNKMGID
                     TLEVLEAAGTKWNFLNFRPGLVGGHCIGVDPYYLTYKAESIGYRPEVILSGRRINDNM
                     GKYIAQKTVKMLIAADLQVKKARVAVLGLSFKENVPDLRNTRVVDIIRELADFGVKVL
                     AHDPLALPEEAREHCGVELAQAKDLKDVDAVILAVAHREYMKWGLEKIASLCRKNRAI
                     LLDVKSVFDPARAQSLGMAYWRL"
     gene            3373..4404
                     /gene="gmd"
                     /locus_tag="PLA58_09155"
     CDS             3373..4404
                     /gene="gmd"
                     /locus_tag="PLA58_09155"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOR62999.1"
                     /translation="MTKALITGITGQDGSYLAEFLLGKGYEVHGLIRRSSTFNTDRID
                     HLYRDFHDPNAKLFLHFGDLSVSGQIMDLLASIRPDEIYHLGAQSHVRVSFDMPEYTG
                     DITGLGTLRILESIRKTGIKTKFYQASSSEMFGAAPPPQSEKTLFQPRSPYAAAKVYA
                     YHIVQNYRDAYGIFATNGILFNHESPRRGETFVTRKITRAATQIKLGLKDKLYLGNLE
                     AKRDWGFAGDFVEAMWLMLQQDKPDDYVIATGETHSVREFAEKVFSKLGLDYAKYVAV
                     DARYFRPTEVDVLLGDASKARKALGWQPRVSFDELVDMMIASDLESAKKEKTLVDAGF
                     SCANTERIS"
     gene            4424..5368
                     /locus_tag="PLA58_09160"
     CDS             4424..5368
                     /locus_tag="PLA58_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOR63000.1"
                     /translation="MLKNKRITVTGGKGFLGRHLINQLSRYGCKSIDIADLPEYNLTR
                     LEDIGRLFDATRPDVIIHLAAKVGGIGFNQEKPAELFYDNLLMGAQLMHEAWVRKIEK
                     FVALGTICAYPKFTPVPFKEDDVWNGYPEETNAPYGLAKKMMLVQSQAYRRQYGFNSI
                     FLLPVNLYGPGDNFDPRSSHVIPALIKKCFDALDRNSDTLEVWGTGSATREFFYVEDA
                     AEAICLAADSYDKSEPVNIGAGFEISIRELTELVAGLSGFSGKIVWDSSKPDGQPRRM
                     LDTSKALKEFGFKAKTDLATGLKKTIAWYRDHRHLLNK"
     gene            5673..6068
                     /locus_tag="PLA58_09165"
     CDS             5673..6068
                     /locus_tag="PLA58_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOR63001.1"
                     /translation="MNLIAKDPLQKKLEMANWVILAVLFIPSVIFAPAKFYLGVLLGG
                     FISILNFHGMALGLQRLFKNLSGNVKRPTMIQYFIRLALTAVVLFLLLTSGTVDAVGL
                     VIGLSVVVINMVFTVITTLSKKNFIEEVG"
     gene            6075..>6331
                     /locus_tag="PLA58_09170"
     CDS             6075..>6331
                     /locus_tag="PLA58_09170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOR63002.1"
                     /translation="MEPLIFFHTSLFKDVGVNMTIVFNTLFIVALLSILSFMASRNLQ
                     IYPGRLQNVMEVVIDGLHSLLVDTMGEHGKKFFPLIATLGL"
BASE COUNT         1502 a         1750 c         1645 g         1334 t
ORIGIN      
        1 ttaccgtgaa atgccagagg gaaagatccc agagtctgaa ccgccatctg gcgcgacgcc
       61 tcctgctgga caaaattgaa agacagcaaa aagggtttgc cgaggaagaa aaggcaaagc
      121 ttgcgaagct ccgccgacag aaaagaaaac gaagccggcg ggcaaaagaa aaaatactcg
      181 ccgctaaaca tcagcaggcc gaaaaaaaag ggctgcgcgg taaagtcatt attgacgaat
      241 gaaatagaaa aatattgaag gtgtcattaa tcccgcggcg cttcgcttcg ggaatttgag
      301 gtgaactaaa aaagggagag aaagtcgatg tacgctcccc ctccctgttt atgatatcaa
      361 gaaaattttt tagccgccaa taacaacctt gaccagcgga ttggcgtaaa ggacgatcag
      421 cgcgataacc agcgcgtaaa tcgccagaga ttcaatcatg gccagaccga ccatcatagt
      481 gagtagaatt ttgccctgag cttcaggatt tctaccgacg gcattggttg cgccgtttaa
      541 tccctggccc atgccattgc cggttccgat ggttccaaaa gcaatcgcga tgcccgccgc
      601 aagcaaagaa cagccaacaa caatggcttt tgtgtaatca acaggtcctg caggagcagc
      661 cgcagccgct tccggagccg ccagaacaaa aggagcggaa acaataacca acacaatcgc
      721 cagcacggaa taaactaaac tctttttcat gaaaaacctc ctcctgtttt taacgaattg
      781 ttttgtccgg attcgtcaag tatgctattt ttttaacctc cttcccttga attttgacga
      841 ctaccggtca tttgagagct gtgttagcga tcaataaacc gattgtcaag aaatattttc
      901 aatcttttct cagacattgt ccgattaccc ggcttgattt tgcgccgcaa gcaggataaa
      961 ggaaaaaaat gaaccgtttc agcacgagac aattaaaagc ggcaaaaatt cttcaatcgg
     1021 acattcctct ctcccccaga ccgtttgcgg acattgcccg ggcatctgaa atgaccagcg
     1081 aagagctgat cgcctggatc cgcaacctct cccgtgaggg catcattcga aaggtcacca
     1141 ccatcctgag acaccagaag gcggggtacg ggaaaaacgt tctggtcctg tggtcggtga
     1201 cgccggaagc cgtcgaagac gcggggcgga aactggccgg actgccgtac atttcccact
     1261 gctacgaaag gaagcccttt gccggccgct acaacctctt caccatgctt cacgcgaagg
     1321 ccggcgacat ttctcacctg cttgaagaga tgtcccggct gatttcatgc cctgattttc
     1381 tgcttctgga aagcctggaa gaatacaaaa aaacgtctcc ggagtatttc tgatgagcaa
     1441 gaataacatc catccgttta ccgaggccgc ccgggtcatt gccggcggcg ttaattcgcc
     1501 ggtgcgcgcc tggaaatcgg tcggcggcga ccctttgttt gtccaacgcg ccgcaggcag
     1561 caaaatctat gacctggacg gaaaagaata catcgactac gttctttcct gggggccgat
     1621 gattctcgga cacgcccacc cggacgtgat tgcggccgtc tgcgatgccg cccggagggg
     1681 aaccagcttc ggcgcgccca cgacggcgga aacggaaatg gccctgctca tccgcgaggc
     1741 catcccctcg atggagcggg tgagaatgac cagttccggg acggaagccg ccatgacggc
     1801 gattcgcctg gcccgcggct gcacgggcag aaacatgatc atcaaatttg acggctgcta
     1861 ccacggccat gccgattccc tgctcatcaa ggccggctcc ggcgtcacga cactgggcat
     1921 tccgggatcg ccgggaattc cggaagcggt ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnntgaccgc ccagtctttt tacgcgggga aaaataaaat cgccattgtc
     2101 ggcctgggct atgtgggcct gccgctggcc gcccacctct ccaggcactt caaggtgatc
     2161 ggctttgaca tcagcgaagc aaacgtcgcg gaactgaaga agggccacga ccgaacgatg
     2221 gagctttccg acgccgaact gaagaaggcc aggatttctt ttacctcccg gccaaacgat
     2281 ctcgcctcct gcaggctgtt catcgtcgcc gttcccacgc cggtggatca ttttcatgtc
     2341 cccgacttaa gcgcgatgga aaacgcgtcg gcgctcatcg ggaaacacat gtccgccggc
     2401 tcctgcgtgg tgtatgaatc gacggtgtat ccgggcgtga cggaagatgt gtgcgttccc
     2461 gttctggagc gggcctccgg gctgaaattc ggcagggatt tcacggtcgg ctattccccg
     2521 gagcgcatca atcccggcga caaaattcac accgtaagca acacgatcaa aatcatctcc
     2581 gcttccgact ccgccacgct ggacctgctg gaaggcatct acagcgcggt cgtcaaggtc
     2641 cgtctgcacc gggtttcctc gattaaagtc gcggaggccg ccaaggtcat cgaaaacacg
     2701 cagcgcgaca tcaatatcgc gctgatgaac gaactggcca tcatcttcaa taaaatgggc
     2761 atcgatacgc tggaagtgct ggaggcggcg ggaaccaagt ggaacttcct caacttccgc
     2821 cccggcctcg taggcgggca ctgcatcggc gtcgacccct actacctgac ctacaaggcg
     2881 gaaagcatcg gctaccgtcc ggaagtgatt ctttcggggc ggcggatcaa cgacaatatg
     2941 ggaaaataca tcgctcaaaa aaccgtcaag atgctcatcg ccgcggacct tcaggtcaaa
     3001 aaagcccgcg tcgcggttct gggcctgtcg tttaaggaaa acgtccccga tctgcgcaat
     3061 acgcgcgtcg tggacattat ccgtgagctt gccgacttcg gcgtgaaggt tctggcgcat
     3121 gatccgctgg cccttcccga agaggcgcgg gagcactgcg gcgtcgagct tgctcaggcc
     3181 aaagacctga aagacgtgga cgccgtcatt ctggcggtgg cccaccggga atatatgaaa
     3241 tggggcctcg aaaagatcgc atcgctgtgc cgcaaaaacc gcgcgattct gctcgacgtc
     3301 aaatcggtct ttgacccggc ccgggcgcag tctctgggga tggcctactg gcgtttataa
     3361 aaaggagaag ctatgacaaa agcattaatc accggcatca ccggacagga cggatcgtac
     3421 ctggccgagt ttctgttggg caagggttac gaggttcacg ggttgatccg ccgctccagc
     3481 acgttcaaca cggaccgcat cgatcatctg taccgggatt ttcatgaccc gaatgcgaag
     3541 ctgtttttgc acttcgggga tctctccgtt tccggtcaga tcatggacct gcttgcctcc
     3601 atccgtccgg acgaaatcta tcatctgggc gcgcaaagcc acgtccgcgt gagcttcgac
     3661 atgcccgagt acaccggaga catcaccggt ctgggcacgc tgagaattct ggaatccatc
     3721 cgcaagaccg gcatcaaaac aaaattctac caggcgtcgt cgagtgaaat gttcggcgcc
     3781 gcgcctccgc cgcagagcga aaaaacgctg tttcaacccc gaagccccta cgcggccgca
     3841 aaggtttacg cgtatcacat cgtgcagaac taccgcgacg cctacggaat tttcgcgacc
     3901 aacggcattc tcttcaacca tgaatcgccc cgccgcgggg aaacgttcgt cacgcgcaaa
     3961 atcacccgcg cggcgacgca aatcaagctg ggcctcaagg acaaactgta tctgggcaat
     4021 ctggaagcca agcgcgactg gggatttgcc ggcgacttcg tggaggccat gtggctcatg
     4081 cttcagcagg acaaaccgga cgactacgtg atcgccaccg gggaaacgca ctcggtccgc
     4141 gaattcgcgg aaaaggtctt cagcaaactg gggctggact acgccaaata cgtggcggtg
     4201 gacgcccgtt atttccgccc gacggaggtg gacgttctgc tgggggatgc gtccaaagcc
     4261 cgcaaggcgc tgggctggca gccgcgggtt tcctttgacg aactcgtcga tatgatgatc
     4321 gcctccgatc tggagtcggc caaaaaggaa aaaacgctcg tggacgcggg attttcctgc
     4381 gccaataccg aacgaatcag ttgatttttg ggagacggcg gtcatgctga aaaacaaacg
     4441 aattacggtg acgggcggca aaggatttct gggtcggcac ctgatcaacc aactgtcccg
     4501 atacggctgc aaaagcatcg acattgccga tctgccggaa tacaatttga cccggctgga
     4561 agatattggc cggcttttcg acgccacccg gccggacgtg atcatccacc tggcggccaa
     4621 agtgggcggc atcggattca accaggaaaa gcccgccgaa ctgttttacg acaatctttt
     4681 gatgggcgcg cagctgatgc acgaagcctg ggtgcgcaaa atcgaaaaat tcgtcgcgct
     4741 gggcaccatc tgcgcctatc cgaaatttac gccggtccct tttaaagaag acgacgtctg
     4801 gaacggctac ccggaggaaa ccaacgcccc ctacggactg gccaagaaaa tgatgctggt
     4861 gcagtcccag gcctaccgcc gccaatacgg ttttaattcc atttttctgc tgcccgtcaa
     4921 cctctacggc cccggcgaca atttcgatcc ccggtcctcc cacgtcattc cggcgctgat
     4981 caaaaaatgc tttgatgccc tggacaggaa ctcggatacg ctggaggtct ggggaacagg
     5041 ttcggcaaca cgggaattct tttacgtcga agacgcggcg gaggccattt gcctggccgc
     5101 cgattcctac gataaaagcg agcccgtaaa catcggagcc ggctttgaaa tttccatccg
     5161 ggagctgacg gaactggttg ccgggttgtc cgggttttcc ggaaaaatcg tctgggattc
     5221 ctccaagccg gacggccagc cccggcgtat gttggataca tccaaagcat tgaaagaatt
     5281 tgggtttaag gcaaagacgg acctcgccac cgggcttaaa aaaaccatcg cctggtacag
     5341 ggatcatcgc caccttttaa ataaatgaac ggcggtcatg tttttgattg actttgactg
     5401 ccccccgtgt taaggaccca ctcaccaaat ggatagcgag accaaaaaat taggcatcca
     5461 gatggcttat gccagcagca tcggcatcgc aatggttctg gcgatttttg gctgcctcct
     5521 tttaggaagc tatctggacc ggaaatttga ttccggccac gcgttcaccg tcgttttcct
     5581 gctcattgga atttcggccg ggtttcgcaa catctatgtt ttgataaaaa gaaatttcaa
     5641 tgatgaagaa atcatcatta agagcttgaa aagtgaacct catcgccaaa gacccgctcc
     5701 aaaaaaaact tgaaatggcc aactgggtca tcctggcggt tctttttatt ccttccgtca
     5761 tttttgctcc ggctaaattt tatctgggcg tcctgctcgg cggcttcatc agcattctca
     5821 actttcacgg catggcgctc ggcctgcaaa gattgttcaa aaatctctcc ggcaacgtca
     5881 agcgcccgac catgattcaa tatttcattc ggctggccct caccgccgtc gtgctgtttc
     5941 tgcttcttac cagcggcacg gtggacgcgg tcggcctggt catcggactt tccgtggttg
     6001 tgattaacat ggtttttacc gtaatcacca ccctgtcaaa aaaaaacttt attgaggagg
     6061 ttggttagac acgaatggag cccctaattt tcttccacac atctttgttc aaggatgtcg
     6121 gcgttaacat gaccattgtt ttcaatacgt tatttattgt cgcacttctg tccatccttt
     6181 cctttatggc ctcgcgcaac ctccagattt atccgggccg gctgcaaaat gtgatggaag
     6241 tcgtcatcga cggccttcac agtcttctgg tggacaccat gggagaacac ggcaaaaagt
     6301 tcttccccct gatcgccacg ctggggcttt t
//
LOCUS       DAOSRM010000108         6297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_11417_length_6297_cov_100.378244, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000108 DAOSRM010000000
VERSION     DAOSRM010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11417_length_6297_cov_100.378244"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="PLA58_09175"
     CDS             <1..1279
                     /locus_tag="PLA58_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOR63003.1"
                     /translation="FFPAFDWAISPNHPEREERLLYTQDQVFEEGLLDFPNIVEYKPD
                     LATYADINRCHICVPNPQYLTTNSHLISAGGAITAAKKVLSGDVDNAFALVRPPGHHS
                     MLVAHGARGFCNINIEAVMIEYIRHQFGVKRIAVVDTDCHHGDGTQDIYWNDPDVLCI
                     SIHQDGRTLYPGTGFTDDFGGPNALGATINIPLPPRTSDEGFLFVMDNVILPILDEFK
                     PEIIINSAGQDNHYSDPITNMSFSAQGYALLNEKLAPDIAVLEGGYSIEKALPYVNVG
                     IILAMAGLDFSQVREPDYDALRIRQSPEITRLIEDEARNVMTLWKKKKNLKKQITGSA
                     AYIRREKNIYYDTDNIMEKQTETVRVCDKCGGVVMIDSAADTGKRIYAIILPNSCCAE
                     CRESGENFFSRMNSSQYNHVYFQDRQKDVFIVK"
     gene            1295..1690
                     /locus_tag="PLA58_09180"
     CDS             1295..1690
                     /locus_tag="PLA58_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOR63004.1"
                     /translation="MEDQKIKEKTQETAEKLFSGGAKLDRPYSLWRAFDRGLANDFSR
                     FITGNLYSREVLSLQERQMAACAMLAALKAREELKVHANAALNVGCDPKKLVEIFFQT
                     ATYAGMPAVNEALAAFREVLQARGEWPLP"
     gene            1697..2488
                     /gene="mazG"
                     /locus_tag="PLA58_09185"
     CDS             1697..2488
                     /gene="mazG"
                     /locus_tag="PLA58_09185"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOR63005.1"
                     /translation="MNQTQTQLQELMALIQRLRAPDGCPWDRKQTRQDIGKYLLDESY
                     ETLDALTENDPGHIQEELGDLLFQILFIAEISAEAGEFTLSDVMKGIREKMIRRHPHV
                     FGDVKVESVQDVRDNWQKIKKREREGSQQTQDPFGNIPRSLPALRRAQKITAAASRCG
                     FDWPDANGVLDKLKEELDELEEARTEGDAAKIEEEMGDLFFTLVNLSRFLNVDAEKAT
                     DRAVNKFLGRWLYITRKLAARGRSPEDATPAELDLIWNEAREKGL"
     gene            2492..2677
                     /locus_tag="PLA58_09190"
     CDS             2492..2677
                     /locus_tag="PLA58_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HOR63006.1"
                     /translation="MDYFLCVIGMVFIIEGLPYLVFPEKLKIYLIKISTLPETVLRVM
                     GIGAIVLGLILLYFGRR"
     gene            2746..3786
                     /gene="queA"
                     /locus_tag="PLA58_09195"
     CDS             2746..3786
                     /gene="queA"
                     /locus_tag="PLA58_09195"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOR63007.1"
                     /translation="MQLKDFSYFLPEELIAQHPRPQRDASRMMRLHRGAKTIEDDLFA
                     ALPKYLERGDVLVINDSRVIPARIYGKKPTGAILELLLLTRREKNDGEELWEVLARPA
                     KRLKEQDIIDLGHSCEGRVVARLTDKKWLMAFRAKNGFENYLERFGRAPLPPYIRRKK
                     SVDPDQTDDRRRYQTIYAKTPGSVAAPTAGLHFSSNVLNDLLARGVQVASVTLHVGPG
                     TFMPIETEQVEDHVMQPEFFEISAETSQKINAAKRVIAVGTTSTRTLESVADKDGHIS
                     PGSGETSLYIYPGYTFKRVNGLLTNFHLPCSSLFLLVSAFAGTDFIRQAYAHAVKNRY
                     LFYSYGDCMLIV"
     gene            3797..4903
                     /gene="tgt"
                     /locus_tag="PLA58_09200"
     CDS             3797..4903
                     /gene="tgt"
                     /locus_tag="PLA58_09200"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOR63008.1"
                     /translation="MPFAFELTGKDPQTRARLGKISTAHGTVDTPAFMPVGTQGTVKS
                     LRPEDLLQCGAQMILGNTYHLYLRPGHETIRQLGGLHAFMNWPGPILTDSGGFQVYSL
                     AALRKIGPEGVMFRSHIDGSKHFLSPRKAVEIQEALGSDIMMCLDECTPYPATFRQTQ
                     DSLALTLQWAKACRGAKTSTHQALFGIVQGGTYPDLRRQAAEEMVPLGFDGYAVGGVS
                     VGEPKVMMYAITDEITPLLPEEKPRYLMGVGMPEDIVYGVSRGIDLFDCVVPTRSARH
                     GLLFTNSEKIVIKNARWREDHSPLDETCDCYTCKHYSRAYLRHLYVAGEILAMILNTI
                     HNIRHYMRLLENIRAAVRENRFGEWEKDFLNKQR"
     gene            4956..5306
                     /gene="yajC"
                     /locus_tag="PLA58_09205"
     CDS             4956..5306
                     /gene="yajC"
                     /locus_tag="PLA58_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOR63009.1"
                     /translation="MISTAFAMGGNQGAPAAQGGFGDWSFIIMMVVIFGIFYFLMIRP
                     QQKKQKELKTMLDNLAYGDMVMTTGGIHGKVTGLADAVVTLEIADKVRIKVARSAIGA
                     VLQKSGQPAPAAKA"
     gene            5325..>6297
                     /locus_tag="PLA58_09210"
     CDS             5325..>6297
                     /locus_tag="PLA58_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOR63010.1"
                     /translation="MFKSLKIRLAITVVVCLVALYFLLPTLVAQIPSPWNNYFPKDKI
                     HLGLDLQGGMHLILEVDADKALESMMERTAGDLKESLMENRVRFRNVEKAKGATITME
                     LSEASGKPALEKLLNEQFSDLEITSSTPREGGQMITLGVKNKRAADLKKLTVEHSVET
                     IRNRVDQFGVAEPEIVPEGENRIMVQLPGIKDPERAKNLIGKTALLEFKIVDEENSLD
                     EALRGNVPEGSIIAYGAREDKASGQRGQVPYLLKNKSLLTGASLETAKVQISDRFGEP
                     HVSMKFNSQGAADFDRITNENVRKRLAIVLDGVVHSAPVIQERISGGQ"
BASE COUNT         1514 a         1742 c         1761 g         1280 t
ORIGIN      
        1 ctttttcccg gcctttgact gggccatttc ccccaaccat ccggagcgtg aagaaaggct
       61 gctctacacg caggatcagg tgtttgaaga aggcctgctg gattttccca atattgtgga
      121 atacaagccg gatctcgcca cctacgccga catcaaccgc tgccatatct gtgttccgaa
      181 cccgcagtat ttaacaacga attctcacct gatttccgcg ggcggcgcga tcaccgccgc
      241 gaaaaaagta ttatcgggcg acgtggacaa cgcctttgcc ctggtccggc cgcccggcca
      301 tcattccatg ctggtcgccc acggcgcgag gggattctgc aacatcaata ttgaagccgt
      361 gatgattgaa tacatccgcc atcaattcgg agttaaaaga attgccgtgg tcgatacgga
      421 ctgtcatcac ggcgacggca cgcaggatat ttactggaac gatccggatg tcctgtgcat
      481 ttccatccat caggacggaa gaacgctcta tcccggaacg ggcttcaccg acgatttcgg
      541 cggccccaac gcgctgggcg cgacgatcaa cattccgctg cccccgcgca catcggatga
      601 aggcttcctt tttgtgatgg acaacgtcat ccttccgatt ctggacgaat tcaagccgga
      661 gatcatcatc aactccgcgg gtcaggacaa ccattacagc gacccgatca ccaacatgag
      721 cttcagcgcc caggggtacg cgctgctcaa tgaaaaactc gcgcccgaca ttgccgtgct
      781 ggagggcggc tactccatcg aaaaagccct gccctacgtc aacgtcggca tcattctggc
      841 gatggccggg ctggacttca gccaggttcg cgagccggat tacgacgctt tgcgcatccg
      901 ccagtcgccg gaaattaccc ggctgatcga agacgaagcc aggaatgtga tgacgctgtg
      961 gaagaaaaag aaaaacctga agaagcagat caccgggtcc gccgcctaca ttcgccgcga
     1021 aaagaacatc tattacgaca cggacaatat catggagaag caaaccgaaa ccgtccgggt
     1081 atgcgacaaa tgcggagggg tggtcatgat tgactcggcc gccgacacgg gcaagagaat
     1141 ctacgcgatc attctgccca atagctgctg tgccgaatgc cgcgaaagcg gagagaattt
     1201 tttcagtcgc atgaacagtt cgcaatacaa ccatgtttat tttcaggacc ggcagaaaga
     1261 tgtctttatt gtaaagtaac ccccgggagc cgccatggaa gatcagaaaa taaaagagaa
     1321 aacgcaggaa acggccgaaa aacttttttc cggcggcgcc aaactggacc gcccttactc
     1381 gctgtggcgc gcctttgacc gcgggctggc caatgatttt tcgcgcttca tcaccggcaa
     1441 cctttattcg cgcgaggtcc tgtcgcttca ggagcgccag atggcggcct gcgcgatgct
     1501 ggcggccctc aaggcccggg aggagctgaa ggtgcacgcc aacgccgctc tgaacgtggg
     1561 ctgcgatccg aagaaactgg ttgaaatatt tttccagacg gcaacctacg ccgggatgcc
     1621 ggccgtcaat gaagcgctgg ccgccttcag ggaagtcctt caggcgcgcg gcgagtggcc
     1681 ccttccttaa ggcatcatga accagaccca aacccaactt caggagctga tggcgctgat
     1741 tcaaaggctg cgcgcgccgg acggctgccc ctgggaccgg aaacagaccc ggcaggatat
     1801 cggaaaatat ctgctggacg aatcctatga gacgctggac gcgctcaccg aaaacgatcc
     1861 ggggcatatt caggaagagc tgggcgacct gctgttccag attttgttta tcgccgagat
     1921 ttcggccgaa gcgggcgagt ttaccctgtc ggatgtcatg aaaggcatcc gggaaaaaat
     1981 gatccggcgg cacccgcatg ttttcggcga cgtcaaggtt gaatccgtcc aggatgtcag
     2041 ggacaactgg cagaaaatca aaaagcggga aagggaaggc tcacagcaaa ctcaagatcc
     2101 cttcggcaac attccccgtt ctctcccggc tttgcgaagg gcgcaaaaaa ttacggcggc
     2161 ggcctcccgc tgcggtttcg actggccgga cgcgaatggg gtgctggata aattgaaaga
     2221 ggagctggat gagctggaag aagcgcgaac agagggcgat gccgcgaaga ttgaagaaga
     2281 gatgggcgac ctctttttta cgctggtgaa cctcagccgt tttctgaacg tggacgccga
     2341 aaaggcgacc gaccgggccg tgaataaatt tctggggcgc tggttgtaca tcacccggaa
     2401 gcttgccgca cgaggccggt cgccggagga cgcgacgccc gcggagctgg atttgatctg
     2461 gaacgaagcc agggagaagg gactatagac attggactat tttctgtgcg tcatcggcat
     2521 ggtcttcatt attgaaggcc ttccctatct ggtttttccg gaaaagctga aaatctatct
     2581 gatcaaaatc tcaacgctgc cggagaccgt gctgcgcgtc atgggcattg gcgccattgt
     2641 cctggggctg attcttcttt atttcgggcg gcgctgacgg ttttcattga cagtcccggc
     2701 ggattctgat tatttgcccc aactttttga cgaaaccagg aaacgatgca gttaaaggat
     2761 ttttcatact ttctgccgga agaactcatc gcccagcatc cgcgtccgca aagggatgcc
     2821 tcgcgcatga tgaggcttca tcgcggcgcc aaaaccattg aggacgacct gtttgccgcc
     2881 ctgccaaaat atctggaacg gggcgacgtt ctcgtcatca acgattcccg cgtgattccg
     2941 gcaagaattt acggaaaaaa accgaccggc gcgatcttgg aactattgct tctgacccgc
     3001 agggaaaaaa acgacggaga ggaattgtgg gaagttctgg cgcgcccggc aaaaaggctg
     3061 aaagaacagg acatcatcga cctcggacat tcctgcgagg gaagggtcgt cgcccggctt
     3121 acggacaaga aatggctgat ggccttccgt gcaaaaaacg gttttgaaaa ctatctggaa
     3181 cgcttcgggc gggctcccct gcccccttac atacggagaa aaaagagcgt agatccggat
     3241 caaacggatg accgccgccg ctaccagacc atttacgcga aaacgcccgg ctccgtcgcc
     3301 gcgccgaccg ccggcctgca tttttcatcg aatgttttga atgatctgct cgccagaggc
     3361 gttcaggtag cgtccgtgac gctgcatgtc ggtccgggga cgtttatgcc gattgaaacc
     3421 gagcaggtgg aagaccatgt catgcagccg gaattttttg aaatcagcgc ggagacctct
     3481 caaaaaatca acgccgccaa acgggtgatc gccgtgggca caacctccac ccggacgctg
     3541 gagagcgtgg cggacaaaga cggccatatt tcgccagggt cgggcgaaac gagcctgtat
     3601 atttaccccg gctacacctt taaacgagtg aacgggcttt tgaccaattt tcatctgccg
     3661 tgctcttccc tgtttctgct ggtttcggcg tttgccggca cggattttat ccggcaggcc
     3721 tatgcccatg ccgttaaaaa ccgttatttg ttctatagtt atggcgactg catgctgatt
     3781 gtataggatg actttcatgc cttttgcttt tgaattaacc gggaaggatc cgcaaacccg
     3841 cgcgcggctg ggaaaaatat ccaccgcgca cggcacggtg gatactcccg cgttcatgcc
     3901 cgtcggcacg cagggaacgg taaagtctct ccgccccgaa gatcttctgc aatgcggcgc
     3961 tcaaatgatc ctgggcaata cgtaccatct ctatctgcgc cccgggcatg aaaccatccg
     4021 tcaactgggc ggtcttcatg cgttcatgaa ctggccgggg ccgatcctca cggacagcgg
     4081 ggggtttcag gtttactctc tggctgcgct gcgcaaaatc gggccggaag gcgtgatgtt
     4141 ccggtcccat atcgacgggt caaaacattt cctgagcccg cgaaaagccg ttgaaattca
     4201 ggaagcgctg ggctcggaca tcatgatgtg cctcgacgag tgcacgccct accccgccac
     4261 gttcaggcag acgcaggatt cgctggccct gacgctccaa tgggccaaag cctgccgcgg
     4321 ggccaaaacc agcacccatc aggcgctgtt cggcattgtc cagggcggaa cctatccgga
     4381 tttgcgcagg caggccgcgg aagagatggt ccccctcggc ttcgacgggt atgcggtagg
     4441 cggcgtcagc gttggcgaac ccaaagtcat gatgtacgcc atcacggacg aaatcacccc
     4501 cctgctgccc gaagaaaagc cccgctatct gatgggcgtc ggcatgccgg aagacatcgt
     4561 ttacggcgta tcccgcggca tcgatctgtt tgactgcgtt gttcccacac gctcggcccg
     4621 gcatggatta ttgtttacaa attccgaaaa gattgttata aaaaacgccc gctggcggga
     4681 agatcattcc ccgcttgacg aaacatgcga ttgctacacc tgtaaacact actcccgggc
     4741 gtatttaagg cacctttatg ttgccggtga gatactggct atgatattga ataccatcca
     4801 taacatccgg cattatatgc gccttctgga aaacatacgg gcggccgtca gggagaatcg
     4861 ctttggggaa tgggaaaagg attttctgaa caagcaaaga tgaaaccata aaaaagggct
     4921 gattcaaagc aacttgattg aaggagggac aaacattgat atctacagca ttcgcgatgg
     4981 gcggcaatca gggcgcgccg gcggcgcagg gaggcttcgg cgactggagc ttcatcatca
     5041 tgatggtggt gatcttcggc attttctact tcctgatgat ccgcccgcag caaaaaaagc
     5101 aaaaggaact caaaaccatg ctcgataacc tcgcatacgg cgacatggtc atgaccaccg
     5161 gcggcatcca cggcaaggtt acgggactgg ccgatgcggt ggtcacgctg gaaatcgccg
     5221 acaaagtcag gatcaaagtc gccagaagcg ccatcggcgc tgttttgcag aagtcaggcc
     5281 agccggctcc ggcggcaaag gcctagcata gaaaggggcg tttcatgttc aaatctttaa
     5341 agatcaggtt ggccatcacg gtggttgttt gccttgtcgc tctgtatttt cttctgccga
     5401 cgctcgtcgc gcagattcct tccccctgga acaattattt tcccaaggat aaaattcatc
     5461 tcggacttga tttgcagggc ggcatgcatc tgattctgga ggtggacgca gacaaagctc
     5521 tggaatccat gatggaaaga acggccggcg acctgaagga atcgttaatg gaaaaccggg
     5581 tccgcttccg caacgtggaa aaggcaaaag gcgctacgat caccatggaa ctttcggaag
     5641 cttccggaaa acccgccctg gagaaacttc tcaatgaaca gttttcagac ctggaaatta
     5701 cctcttccac gcctcgcgaa ggcggacaga tgatcaccct gggcgtcaaa aacaaaaggg
     5761 ccgcggatct gaaaaaactg accgtggagc acagcgtcga aaccatccgc aaccgtgtgg
     5821 accagttcgg cgttgccgag ccggaaattg ttccggaagg cgaaaaccgc attatggtcc
     5881 agttgccggg catcaaggat ccggaacggg ctaaaaacct catcggaaaa acggcgcttc
     5941 tggaattcaa gatcgttgac gaggaaaatt ctctggatga agccctgcgc ggcaatgttc
     6001 cggaaggctc catcatcgcc tacggcgcgc gggaagacaa agccagcggc cagaggggcc
     6061 aggttcctta cctgctgaaa aacaaaagcc tgctcaccgg cgcgtcgctg gaaaccgcca
     6121 aggtccagat ttccgaccgg ttcggcgagc cgcacgtctc catgaagttc aactcgcagg
     6181 gcgcggccga tttcgaccgc atcaccaatg aaaacgtccg caagcgtctg gccatcgtgc
     6241 tggacggcgt ggtccattcg gcgccggtca ttcaggaaag aatctccggc ggccagg
//
LOCUS       DAOSRM010000109         6283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_11456_length_6283_cov_97.882627, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000109 DAOSRM010000000
VERSION     DAOSRM010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11456_length_6283_cov_97.882627"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(383..601)
                     /locus_tag="PLA58_09215"
     CDS             complement(383..601)
                     /locus_tag="PLA58_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOR63011.1"
                     /translation="MSKVLNVRLTDDLSSRLDFLAEKTKRPKSFYIKEILSSYLPEFE
                     DAYLALDRLNDRNAKYYSTEDVEKVLDL"
     gene            complement(1237..2649)
                     /locus_tag="PLA58_09220"
     CDS             complement(1237..2649)
                     /locus_tag="PLA58_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOR63012.1"
                     /translation="MTGQEKDLFTKELSRRKVLKIAGTAALAGMMGPLAARRLWAGEK
                     KPNILFILTDDHRYDAFSCMGHPVVKTPNLDRIANEGAHFRNTFVTTSLCSPSRASFL
                     TGQYAHTHGVVTNHTMWNDRNVTFMELIKKAGYDTAFIGKWHMPGDRLPQLRGVDEFI
                     TFTKEGGQGVYYDCPLIIKGVETPRPGKYITNDLTDFALRFIEKKRKNPFCLYLSHKA
                     AHFGFRPPEHLKTLYAGQDLHLPPESDTWVTLTNNNIFVGAPMPMNMLYRNYLRVVAS
                     VDEQVGRLLESLERTGELDNTVIVYAGDNGHLFGEHGLYDKRNAYEESMRIPFFIRYP
                     KLVRQTGRRDQMVLNIDLAPTLLDIIGAEIPAFMQGRSMVPFLRNPSLEGRDAFLYEH
                     FPVFPIPIPGITAVRTKDFKYINYQKGTWRDQLFDLRKDPRELLNIIGTKAGKEALPG
                     LQKRLEQLKEETRYRFFTHG"
     gene            complement(2646..3224)
                     /locus_tag="PLA58_09225"
     CDS             complement(2646..3224)
                     /locus_tag="PLA58_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63013.1"
                     /translation="MEKKPVFQRVVFPAAVVLALMIVSVNLYNASRWWEPPILQAFFI
                     HLSALGMFASIWLGAMIANTLAFFRGATFGERLLVCLVTPMVWDAKVLFDFIGIFSGA
                     EVLYACFHAVIMGPIFVALLCMGISEIWCRIIYRRRTGDRFVKIMDFKSGLVLIVGLV
                     MAFLLLYNGGHSFYYLYMDVYSRIFLSPGGMQ"
     gene            complement(3369..4511)
                     /locus_tag="PLA58_09230"
     CDS             complement(3369..4511)
                     /locus_tag="PLA58_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOR63014.1"
                     /translation="MNGRLAPESISLLLKPASSLCNLACDYCFYRRTEELYPGHATKM
                     TRKILETVIRKTLSVASPFNSFCWQGGEPLLMGIDFFRDVAAFQKKYARPGQIIENSL
                     QTNGLLLDAPWCEFLRKENFLVGVSLDGPAEIHNFYRKDYAGHGSFDGVMRGIGLMQE
                     HEVSFNILCLLTDQNIQSPLKLYDFFLRNGFKFLQFIHCFETNPQTGELMPFSVRAEE
                     TGEFYIKLFDEWLEKNFYDVSIRFFEDLLLYLVDGVKASCCYQKTCSSYLVVEHNADC
                     YPCDFFVSGEWKLGNLLESRLPTLMNSARRCSFARLKSDRPEECKNCPIVSFCMGDCT
                     RFRFRRDSGHQNVSEYCAAIKMVYRHMEPHLPEIKNRVAAFRGGRM"
     gene            4893..5690
                     /locus_tag="PLA58_09235"
     CDS             4893..5690
                     /locus_tag="PLA58_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR63015.1"
                     /translation="MIGIDMTGRVTLITGAGGGIGGGIADVFAKAGARVYVADLEMEN
                     AKAKAGQLRAEGLQAEAVGLDVTKKEEIDALVDRMVKENGKIDHLINCAGISYNKPYM
                     ESTDDEFRSILEVNLISVHNCCQAVLRQMIPRKEGKIVNILSASSRMGSDFFAHYSSS
                     KFGLLGLTQSIGLAAARHNINVNGICPGLIETGLGEKQGGGLIQQMSRYSGKSQDVLR
                     ENLRKSIPLRRFQSGEDIGYMALFLCSDLAKNITCQAINVCGGMRMN"
     gene            complement(5966..>6283)
                     /locus_tag="PLA58_09240"
     CDS             complement(5966..>6283)
                     /locus_tag="PLA58_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63016.1"
                     /translation="AYTLKECFHNKGVTASGEPVRKGIVAVSRDLERKGLTLGTKIQI
                     GNMGTFVIKDRMNRRKKGNIDIYMTSYTDALKFGKQKYTLVRKQRASALPENQKQYVW
                     ALN"
BASE COUNT         1542 a         1530 c         1614 g         1597 t
ORIGIN      
        1 gggcgctaga accgtcccag gtgggttcga ttcccatgca cttccgccaa ttttaattga
       61 ttttattatg gttttggctt tcataaaccc gcccggctta aaatcatcac aggactaatt
      121 ttttttaaca ggatcatcgt aaacgttttt tctgtctgct attctcaata tcagaataat
      181 tacggaatct ccgtctatgg cgtaaatgat gcggtatgat ccgtagcgat aacgataaag
      241 acccttaaag attccctgga gcggcttccc tagacttaaa ggatcctttg aaagataatc
      301 ttttatcctg gcgatgatct tcttttgtgt ttgccggtca atttttttaa gatcacttat
      361 tgcttcttca tgccaccgga tattataagt caagaacctt ctccacatct tctgttgagt
      421 aatatttggc atttctgtca ttgagccgat caagggcaag ataggcgtct tcgaactcag
      481 gaagatacga gctaagaatt tcttttatat agaagctttt tggccttttt gttttttcgg
      541 ctaaaaaatc aagccgggat gataggtcat ctgttagacg gacgtttaat actttggaca
      601 tggtttaacc tctctatgtg tcatatatga attacttgtg ttacacacta acacattgca
      661 gcacataaga tcaagctata ttgttacagt tcaatcggcc aatattttta tgccgctttc
      721 ctctttgatt ttctcaaata ttacaatttc agactctccc ggaacccgcc cgggttgaaa
      781 attgcccctg attttatcaa taatatttaa tgcttataaa ttggtgtaag cattcgattc
      841 ccatgcactt ccgccaattt attagcaaaa ctcgattttt atcgaataaa attctttaac
      901 cttacggtgt tagtatctca ctaagctttc taaatgaaaa aaggcggatc acttatgacc
      961 cgcctttcca atggtggttt caaattatgg ttaacctttg taaccgctcc aactggcgct
     1021 tgtaacgcaa ttacaaatgt ttctttatgt ctcttcgatg accttacaac tatctcgcca
     1081 ttagaagcgg ttctccggcg acaatcatct tctttatttc aacttgaacg tgatgatacc
     1141 atataataat aaaaaatcaa ggaaataatc aatatatagt ggttgtcatg aataataaca
     1201 caataagtag tgttcacggc catttccgta atgcacctag ccgtgcgtga aaaatcggta
     1261 acgggtttct tctttgagct gttccagcct cttttgcagg ccgggcagcg cctctttgcc
     1321 ggctttcgtg ccgatgatat tgagaagctc ccgcggatcc tttcgcagat caaagagctg
     1381 atcgcgccag gttccttttt gataattgat gtatttgaag tcttttgtcc ggacggccgt
     1441 aatgccggga atcgggatcg gaaagaccgg aaaatgttca taaaggaagg cgtcccgtcc
     1501 ttcaagagaa ggattccgta aaaagggcac catgctccgg ccctgcatga aagccggaat
     1561 ctccgcgccg atgatatcca gaagcgtcgg cgccaggtcg atgttcagaa ccatctgatc
     1621 tcttctgccg gtctgtctca ccagtttcgg atagcggatg aagaaaggaa ttcgcatcga
     1681 ctcctcgtag gcgttgcgtt tgtcgtaaag tccgtgctca ccgaagaggt gtccgttgtc
     1741 gcctgcataa acgatgacgg tgttatccag ctcgcccgtc cgctccagcg actcaagcag
     1801 acgacccacc tgttcgtcaa cggaggccac gacccgaaga taattgcggt agagcatgtt
     1861 catgggcatc ggcgcgccga caaaaatatt attgttcgtg agcgtcaccc aggtgtccga
     1921 ttcggggggc aagtgaagat cctgccccgc atagagcgtt ttcagatgct ccggcgggcg
     1981 gaagccgaaa tgcgcggcct tgtgcgacag gtacaggcaa aagggattct ttcttttctt
     2041 ttcgatgaac cgaagggcaa aatccgtcag gtcgttggtg atgtacttgc ccggccgcgg
     2101 cgtttcgacg cctttgatga tcagcggaca gtcgtaataa acgccctggc cgccttcctt
     2161 ggtgaaggtg ataaactcat caacgcctct taattgcggc aggcggtcgc ccggcatgtg
     2221 ccatttgccg atgaaggccg tgtcgtagcc ggcctttttg atgagctcca tgaaggtaac
     2281 atttcggtca ttccacatgg tgtgattcgt gaccacgccg tgcgtgtgcg catactgacc
     2341 cgtgagaaaa ctcgcgcgcg acgggctgca aagcgatgtc gttacaaagg tgtttcggaa
     2401 atgggcgcct tcattggcaa tgcggtccag gttgggggtt ttcaccacgg gatggcccat
     2461 gcaactgaag gcatcgtaac gatgatcgtc ggtcaggata aaaaggatgt tgggcttttt
     2521 ttcgcccgcc cagagccgcc ttgccgcaag cggtcccatc attcccgcaa gcgccgcggt
     2581 tcccgcgatc ttcaggacct ttcgccgcga cagctctttt gtaaacaaat ctttttcctg
     2641 gccggtcatt gcatgcctcc cggacttaag aaaattctgg agtaaacgtc catgtaaagg
     2701 tagtagaaac tgtgcccgcc gttgtaaagc agaaggaagg ccatgacaag gccgacgatc
     2761 agcaccagcc ctgatttgaa atccatgatt ttgacaaaac ggtcgccggt gcgccttcgg
     2821 taaatgattc tgcaccagat ttcggaaatt cccatgcaca aaagcgcgac gaaaatcggg
     2881 cccataatga cggcatggaa gcaggcataa agaacttccg cgccggaaaa gatgccgatg
     2941 aaatcaaaaa ggactttggc atcccagacc atcggggtca ccaggcagac cagcagccgc
     3001 tcgccgaagg ttgcgccccg gaagaaggcc agggtattgg cgatcatggc gcccagccag
     3061 atgcttgcaa acatgcccag cgctgaaaga tgaatgaaga acgcttgaag aatcggaggc
     3121 tcccaccagc ggcttgcatt gtaaagattc accgagacga tcatcagggc aagaacaacc
     3181 gctgcgggaa aaacgactcg ctggaatacc ggctttttct ccatggaacg gtcctcaaaa
     3241 atccacggtc aaaggtggag atcattttct cccgccgaac gccgcgaatc gcggcggttc
     3301 ggtattctta aaagcgtgag gaagtataaa agcaaccccc ttttgtgtca accggttttc
     3361 ggccacactt acattcgtcc gccgcggaaa gcggcaacgc ggtttttgat ttcgggcaga
     3421 tgcggctcca tgtgacgata aaccatttta atggcggcgc agtactcact gacattttga
     3481 tggcccgaat cccggcggaa cctgaacctt gtgcagtccc ccatacaaaa agaaacaatg
     3541 gggcagtttt tgcactcctc cggccggtcg gacttgagtc tggcaaagga gcatcgccgg
     3601 gcgctgttca tcagcgtcgg aagcctgctc tccagcaggt tgccgagttt ccactccccg
     3661 gatacgaaaa agtcgcaggg atagcagtcc gcgttgtgct cgacgacgag ataactggag
     3721 caggttttct gataacagca cgaggccttg accccgtcga caagatacaa aagcaggtct
     3781 tcgaaaaacc ggatggaaac atcatagaag tttttttcga gccattcgtc gaacagcttg
     3841 atatagaatt caccggtttc ttccgcccga acggaaaagg gcatcagctc ccctgtctgc
     3901 gggttcgttt caaaacaatg gatgaattga aggaatttaa aaccgttgcg taggaaaaaa
     3961 tcatagagct tcagcggcga ttggatgttt tggtcggtga gcaggcacag gatattgaaa
     4021 gagacttcgt gctcttgcat cagcccgatg ccccgcatga cgccgtcaaa gctgccgtgt
     4081 ccggcgtaat ctttccggta aaagttgtgg atctccgccg gaccgtcgag gctgacgccg
     4141 accagaaagt tttctttgcg gagaaactcg caccacgggg catccagcag caggccgttg
     4201 gtttgcaggg aattttcaat gatctgtccc ggccgggcgt attttttttg aaaggccgca
     4261 acatcccgga aaaaatcgat tcccatcagg agcggttccc ctccctgcca gcagaagctg
     4321 ttgaaagggg aagcgacgga cagggttttt cgaatgaccg tctccagaat tttgcgggtc
     4381 attttggttg cgtgtccggg ataaagctcc tccgtccgcc ggtagaagca atagtcgcag
     4441 gcaagattgc acaggctgga ggcgggcttt aacagcagcg atatcgattc gggagctaaa
     4501 cgaccgttca tgaaatttta caatcctttc gttgcatact tccatgtttt cagagagaat
     4561 ccggcgaaag agtcctgaaa cctttcaaaa cctgctcaag cgtttgccgc tgtctttgct
     4621 cattcatttt ttagcgtaga atgattcaat tgtccacttc ttgtcttata tttgcgcttt
     4681 gagcgcgtca cacaatgccg acagggcaat gatgataagg atattttcgc aaagggtttc
     4741 attcattctt cttgcaatgt attcggttgt atgatttgcc cggcatcatc agagaaatac
     4801 aaatcttatg aggcgtggaa agattggatc gcggggcttg gttgtaagcc cattgaaacc
     4861 gtaaatccta acgtgtcggg gaggcaagga cgatgatcgg aatagacatg accggccggg
     4921 tgacgctgat cacgggcgcc ggcgggggaa tcggcggggg catcgcggat gtgttcgcga
     4981 aagccggcgc cagggtttat gtggcggatc tggaaatgga aaacgcaaaa gcaaaagccg
     5041 gacagttgcg ggcggaaggt ttgcaggcgg aggctgtcgg actggacgtg accaaaaaag
     5101 aagagattga tgcgctggtt gatcggatgg tcaaggagaa cggcaaaatc gaccatctca
     5161 tcaactgcgc cggaatatcc tacaacaagc cgtacatgga atcgacggat gatgaatttc
     5221 gaagcattct ggaagtcaac ctgatcagcg tgcacaactg ctgccaggcg gttctccggc
     5281 agatgattcc gcgcaaagaa ggcaaaatcg tgaatatcct ctcggcttcc tcccggatgg
     5341 gcagcgactt ttttgctcat tattcgtcgt cgaaattcgg actgctcggc ctgacccaga
     5401 gcatcggcct ggcggccgcg cggcacaaca tcaatgtcaa cgggatctgc cccggcctca
     5461 ttgagacggg gctgggggaa aaacagggcg gtggcctgat tcagcagatg tcccggtact
     5521 ccggaaaatc gcaggatgtg ttaagagaga atctgcggaa atccatcccg ttgcggcgat
     5581 ttcagtccgg cgaggatatc ggctacatgg cgctgtttct gtgctccgat ctggcgaaaa
     5641 acatcacctg ccaggcgatc aacgtctgcg gcggaatgag aatgaactga gtgtcggcgg
     5701 cgcctgcgat ttgggatgtg gcgaacacaa gacttgcggc aatgaaggca aacacaaaga
     5761 ttttacgcat atggatgacc ccccgaaaac agtgatggcg gggcattcta tgagtttcca
     5821 atgggccagt ctatgaaaaa cagaaccgcc cttaatttgg tctttcaggg gagcgtgttg
     5881 aagatgcgag tgattcaggg tttttgccat ccgggggtga ggagctcata cccccggatg
     5941 gccttgtgct gcggttgacc cgccgttaat tcagagccca gacatactgc ttttgattct
     6001 ccgggagagc ggacgctctc tgctttcgaa ccaacgtgta tttctgtttt ccaaatttta
     6061 gtgcgtctgt gtatgatgtc atgtaaatgt cgatgtttcc ctttttcctg cgattcatcc
     6121 ggtccttgat gacgaaagtc cccatattgc cgatttgaat tttggttcct aatgtcaatc
     6181 cttttcgttc cagatcccgg gagacggcca cgatgccttt tctgacgggt tctccggaag
     6241 cggttacgcc tttgttgtgg aaacactcct tcaacgtgta cgc
//
LOCUS       DAOSRM010000110         6197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_11638_length_6197_cov_115.303159, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000110 DAOSRM010000000
VERSION     DAOSRM010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11638_length_6197_cov_115.303159"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1799
                     /gene="trpE"
                     /locus_tag="PLA58_09245"
     CDS             297..1799
                     /gene="trpE"
                     /locus_tag="PLA58_09245"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOR63017.1"
                     /translation="MYSPKFADFEILAQRGNLIPVYREILADLETPVSVLKKLQNKNH
                     VYLLESVEGGEKWGRYSFIGTDAGVVFKIRGPNVILEEKGKISEIGHAGDPLGRMRDL
                     LGRYRPAVPPGLPRFFGGAVGYLGYDMVRYFEKLPDAPPDDLNLDESVFVISDSLIIF
                     DNARHTIKVVACACVEDADSSEDAYTAACRKIDDLIALLQRPSALPQPSEKTSGVIFE
                     SNMTPDRYKAIVGKAKDYIAAGDAIQVVLSQRFSTVCPSDPVDLYRALRYVNPSPYLF
                     FLKLDDLTLIGSSPEVMVRLEQNDVELRPIAGTRPRGKTEQEDRALADELLSDEKERA
                     EHVMLVDLGRNDLGRIAQAGSVQVNQFMVVEKYSHVMHLVSNVRAQLAKGKDAFDVLT
                     AAFPAGTLSGAPKIRAMQIIDELEPARRGVYGGAVGYIGFSGNMDLCITIRTMVLRNG
                     RIFIQAGAGIVHDSMPESEHRETLQKARGMQKAVELACGGFVLENGRSAV"
     gene            1796..2380
                     /locus_tag="PLA58_09250"
     CDS             1796..2380
                     /locus_tag="PLA58_09250"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOR63018.1"
                     /translation="MILMIDNYDSFTFNIVQYLGQMGEDVRVCRNDRTTPGEIRRQRP
                     EAIFLSPGPRTPREAGITVDVIREFHACVPLLGICLGHQSIGYAFGGEVVRAERIMHG
                     KVSPVEHDGKTIFAGLPNPFPAGRYHSLLVRPESLPSCLEVSARTAEGEIMGLRHRDY
                     PVEGIQFHPESVLTPQGKRIFRNFLKHIRRKEHG"
     gene            2377..3390
                     /gene="trpD"
                     /locus_tag="PLA58_09255"
     CDS             2377..3390
                     /gene="trpD"
                     /locus_tag="PLA58_09255"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOR63019.1"
                     /translation="MMIREALDKVVQKIDLPEGEMMAVMEEIMEGKATPAQIGSLATA
                     LRMKGETVEEVTGAARIMRRKAARVNACATTIVDTCGTGGDKLGTFNISTTTAFVVAA
                     AGIVVAKHGNRAVSSGCGSADVLEALGVNISVDQEIVEECLQQIGIGFLFAPKLHGAM
                     KYAVGPRREIGIRTIFNMLGPLTNPAGATAQLLGVYDAKLTEMFAGVLKNMGTKRAFV
                     VHGADGLDEVTITGETRVAELKDGVIRAYNISPKEFVGATYPLEAIRGGDPSVNARIL
                     TDVLSGTPGACRDIVLMNAAPAIVAGEKAADIREGVEIAADCIDSGRAVKKLQALIEM
                     SNS"
     gene            3532..4317
                     /gene="trpC"
                     /locus_tag="PLA58_09260"
     CDS             3532..4317
                     /gene="trpC"
                     /locus_tag="PLA58_09260"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOR63020.1"
                     /translation="MILDKIIETKKEEVVRLKKETSLERLRDAIGGLPPCRDFRQAIS
                     GGSCSVIAEVKCASPSRGRMVKHFDPAAIAGLYEQNGAAAISVLTDETYFSGQKNDLM
                     RIGRNVRLPLLRKDFIIDPLQIYETRAIGADAILLIVRVLGPGLSEFVALSEELGLNP
                     LVEVHTEAELDAALGAKAGMIGINNRNLDTFVTDIRTSCELFKRIPEGPVVVAESGLK
                     NRADIEFLMNAGISAFLIGEALVTAPDIGRKLRSFLGRELSCR"
     gene            4308..>4504
                     /locus_tag="PLA58_09265"
     CDS             4308..>4504
                     /locus_tag="PLA58_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63021.1"
                     /translation="MQVKICGITSLEDALLTAENGAAAVGFVFYPPSPRFIEPQKAAG
                     ISKALPARLVRVGVFVNAKES"
     assembly_gap    4505..4604
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4605..4814
                     /locus_tag="PLA58_09270"
     CDS             <4605..4814
                     /locus_tag="PLA58_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63022.1"
                     /translation="PLILSGGLNDSNILEALQKVRPAALDINSGVEERPGKKEAAKIK
                     NIMQLIKHATDGNPSPPIFVQREEK"
     gene            4811..5998
                     /gene="trpB"
                     /locus_tag="PLA58_09275"
     CDS             4811..5998
                     /gene="trpB"
                     /locus_tag="PLA58_09275"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOR63023.1"
                     /translation="MKTLPDQNGHFDVFGGRYAAETLMPALLELQEAYAKAQKDKTFA
                     EEFDRCRREYAGRPTPLYYAERMTRMLGGAKIYLKREDLNHTGSHKINNTLGQALLAR
                     RMGRKKVIAETGAGQHGVATATVAALFGMECKIFMGEEDIRRQAPNVFRMKILGAEVV
                     PVKSGTATLKDAMNEAMRYWVSSVRDTFYIIGTTAGPHPYPMMVRNFQSVIGREVKKQ
                     TVKAEGRNPDVLVACVGGGSNAMGLFYPFRHEKKVEMFGVEAAGRGMNTNEHSASING
                     GKIGVLHGNKTYLLQDENGQVRDAYSIAAGLDYPGVGPEHAYFAQSGRAAYVTVTDEE
                     AIGAFLLLSRQEGIIPALESSHAIAHAMKIAPKMKKSQILVVCLSGRGDKDARTIMET
                     LQG"
BASE COUNT         1361 a         1690 c         1858 g         1188 t
ORIGIN      
        1 ggtgtgggtt gctttttacg aggtcgtcaa aaatgggttg acagaaattg gtcccgctgt
       61 tatagaacgc gcaaattgat tgcgggaaag tgtgcaatga aaaaaatcgt cggaatgaat
      121 tcagcaaata actggtggtg gtggaacctc ccggagagga gcatccgccg ctgatcttta
      181 ttagataaaa gtcaaagccg tggagccctc tcaggactcc acggcttttt ttatgcacca
      241 accggaaagg ccgtgacgca acgcacggcc ttttttattt tccacaagga gaaaatatgt
      301 attcaccgaa attcgcggat tttgaaattc tcgcccaacg gggaaacctg attcccgttt
      361 accgggaaat cctggcggac cttgaaacgc ctgtttccgt cttaaaaaaa ctgcagaaca
      421 agaaccatgt ttatctgctg gaaagcgttg aaggcggcga aaagtggggg cggtattcct
      481 ttatcgggac ggatgcgggt gtcgtgttta aaatccgcgg tccgaacgtg atcctggaag
      541 aaaagggaaa aatctccgag atcgggcatg caggcgaccc gctgggcagg atgcgggatc
      601 tgctgggccg ctaccggccg gcggtccctc ccggactgcc gcgttttttc ggcggcgccg
      661 tgggctacct gggctacgat atggtgcgct atttcgaaaa gcttcccgat gcgcctcccg
      721 atgacctgaa tctggatgaa tccgtttttg tcatcagcga ttctctgatc atctttgaca
      781 atgcgcgtca cacgatcaag gtggtggcct gcgcctgcgt tgaagacgcg gattcctccg
      841 aagacgccta cacggccgcc tgccgaaaga tcgacgatct gattgcgctg ctgcagaggc
      901 cttcggccct gccgcaacca tcggagaaaa ccagcggcgt gatctttgaa tccaatatga
      961 cgcccgatcg gtacaaggcc atcgtgggaa aggcgaagga ctacatcgcc gcgggagacg
     1021 ccattcaggt ggtgctctcc cagcgctttt ccacggtttg tccttccgat ccggtcgatc
     1081 tttaccgggc gcttcgctac gtgaacccat ccccctatct gtttttcctc aagctggacg
     1141 atctgaccct gatcggctca tcccctgaag tcatggtgcg cctggagcaa aacgacgtgg
     1201 agctgaggcc gattgccggc acaaggccgc gcggcaagac cgaacaggag gaccgcgcgc
     1261 tggccgatga gctcctctcc gacgagaagg agcgggccga acatgtcatg ctggttgacc
     1321 tcggcagaaa tgacctgggc cggatcgcgc aggccggctc ggtgcaggtc aaccagttca
     1381 tggttgtgga aaaatattcg cacgtcatgc acctggtctc caatgtgcgg gcgcagttgg
     1441 ccaaaggaaa ggacgccttc gatgtgctga ccgccgcgtt tccggcggga accctctccg
     1501 gcgcgcccaa aattcgcgcc atgcagatca tcgacgaact cgaacccgcg cggcggggcg
     1561 tttatggcgg cgcggtgggc tacatcggct tcagcggcaa catggatctg tgcatcacca
     1621 tccgcaccat ggtcctgcgc aacgggcgga tttttattca ggccggcgcg ggcattgtcc
     1681 acgattccat gcccgaatcc gagcaccggg aaacgctcca gaaagcgcgg ggcatgcaga
     1741 aggccgtgga gctggcctgc ggcggattcg ttctggaaaa cgggaggagc gctgtatgat
     1801 tctgatgatc gacaattacg attcctttac gttcaacatc gttcagtacc tgggacagat
     1861 gggggaagac gtccgggtct gtcgcaatga ccggacaacc cccggggaaa tcaggcggca
     1921 aaggcccgag gcgatttttt tatcgcccgg tccgcgcacc ccccgggaag cgggcatcac
     1981 cgttgacgtc attcgggagt ttcatgcctg tgtgccgctt ttgggcattt gcctggggca
     2041 tcagtccatc gggtatgcgt tcggcggaga agtcgttcgc gccgaacgca tcatgcacgg
     2101 aaaagtatcg cccgtggagc acgacggcaa aacgatcttt gcggggctgc ccaatccttt
     2161 ccccgccggg cggtatcatt ccctgctggt gcggccggaa tctctgccgt cctgccttga
     2221 agtgagcgcc cggaccgccg agggggaaat catgggactg cggcacaggg actatcccgt
     2281 ggaaggaatt cagtttcacc cggaatccgt gcttaccccg caggggaaaa gaattttcag
     2341 aaacttttta aaacatatcc gacgaaagga gcatggatga tgatcaggga agccctcgac
     2401 aaagttgtgc aaaaaatcga tctgccggag ggagaaatga tggccgtcat ggaagagatc
     2461 atggaaggca aagccactcc cgcgcagatc ggctcgctgg ccaccgcgct gcgcatgaag
     2521 ggcgaaacgg tggaggaagt gaccggcgcg gcgcgcatca tgcgccggaa ggccgcgcgc
     2581 gtgaacgcct gcgcgacgac catcgtcgat acctgcggca ccggaggcga caaactgggc
     2641 acgttcaata tttccaccac gacggctttc gtcgtggcgg cggcgggcat tgtggtggcc
     2701 aaacacggca accgggccgt ctccagcggg tgcggcagcg ccgatgtgct ggaagccctc
     2761 ggcgtcaaca tcagcgtcga tcaggaaatc gtcgaagagt gcctccagca gatcggcatc
     2821 ggcttcctgt tcgcgccgaa gctgcacggc gcgatgaagt atgccgtcgg cccgcgccgt
     2881 gaaatcggca tccgcacgat cttcaacatg ctgggaccgc tcaccaatcc tgccggcgcg
     2941 accgcgcagc tgctgggcgt ttacgacgcg aagctcaccg aaatgttcgc cggtgttctg
     3001 aaaaacatgg gaacgaagcg ggcctttgtg gtgcacggcg ccgacggcct cgatgaagtc
     3061 acgatcaccg gggaaacccg cgtggcggag ctcaaagacg gcgtcatccg cgcgtacaac
     3121 atttccccga aggagttcgt cggggccaca tatccgcttg aggcgatccg cggaggcgac
     3181 ccgtcggtca acgcccggat tctgacggat gtgctctccg gaacaccggg cgcctgccgg
     3241 gatatcgtgt tgatgaatgc ggcgccggcg atcgtggccg gtgagaaagc ggcggatatc
     3301 cgggaggggg tggagattgc cgccgactgc atcgacagcg gccgggcggt aaaaaaacta
     3361 caggcgctca tcgagatgag caacagttaa ttcattttat cgtcattgcg agcagcccct
     3421 tgggctgcgc ggcaatccga cgtagtcgtt gcgaggacgc agtcagcggc aatctcggga
     3481 gacggcttcg tcgctttgct cctcgccatg acacgcgcaa ttggatgaag aatgattctc
     3541 gacaaaatta ttgaaaccaa aaaagaggaa gtcgtcaggc tcaaaaagga gacatcgctt
     3601 gaacgtcttc gcgatgcgat tggcgggctt cctccctgcc gggacttcag gcaggcaatc
     3661 agcggcggga gctgctccgt catcgccgaa gtgaaatgcg cctcgccttc ccgcgggcgt
     3721 atggtgaagc atttcgatcc ggcggcgatc gccgggctct atgagcaaaa cggcgcggcg
     3781 gcgatttccg ttctgaccga cgagacgtat ttttctggcc agaaaaatga tctgatgcgc
     3841 attgggcgca acgttcggct tcccctgctg cgcaaggatt ttatcatcga cccgctgcag
     3901 atttacgaaa cgcgcgccat cggcgcggac gcgattctgc tgattgtccg cgtgctgggg
     3961 cccgggcttt ccgaatttgt cgccctttcc gaagaactgg gattaaaccc gctggtcgag
     4021 gttcataccg aggcggagct cgatgcggcg cttggggcaa aagccgggat gatcggcatc
     4081 aacaaccgca atctcgatac gttcgtgacg gacatccgca ccagttgcga acttttcaaa
     4141 cggattccgg agggtcctgt ggtcgtcgcg gagagcggcc ttaaaaaccg cgccgacatc
     4201 gaatttttga tgaacgccgg tatttcggcc tttcttatcg gcgaagcgct ggtgaccgcg
     4261 ccggacatcg gcagaaagct gcgatcgttt ctggggaggg agttgtcatg caggtgaaga
     4321 tttgcggcat caccagcctt gaagacgcgc ttttgacggc cgaaaacggc gctgcggccg
     4381 tggggtttgt tttctatccg ccttccccgc gcttcatcga accgcaaaaa gcagccggga
     4441 tttcaaaggc gctgccggcc cgactggtca gggtcggcgt cttcgtcaat gcaaaagaat
     4501 cctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgctg attttatcgg
     4621 gcggtttgaa cgactccaac attcttgagg cgctgcaaaa ggtccgtccg gctgcgctgg
     4681 acatcaacag cggcgtggag gaaaggccgg gtaaaaaaga ggcggcaaaa atcaaaaaca
     4741 ttatgcaact cattaagcac gcgacggacg gcaacccgtc gccgccaata tttgttcaaa
     4801 gggaagaaaa atgaaaacac tgccggatca aaacggacac tttgatgttt ttggaggacg
     4861 gtatgcggct gaaacgctga tgcccgcgct gctggagctc caagaggctt atgcgaaggc
     4921 tcaaaaagac aaaacctttg cggaggaatt cgaccggtgc cggcgcgaat acgccggacg
     4981 accgacaccg ctttattacg ccgaacgaat gaccaggatg ctcggcggcg ccaaaatcta
     5041 tctcaagcgc gaagacctca atcacaccgg ctcgcacaag atcaacaaca ccctggggca
     5101 ggcgcttctg gcgcggcgca tgggcaggaa aaaagtcatt gccgagaccg gcgcgggaca
     5161 gcacggcgtg gcgaccgcaa ccgtggcggc gcttttcgga atggaatgca aaatcttcat
     5221 gggcgaggaa gacatccgcc gccaggcgcc caacgtcttc cgcatgaaga ttctgggcgc
     5281 ggaggtagtg ccggttaaaa gcggcacggc cacgctcaag gacgcgatga acgaggccat
     5341 gcgctactgg gtgtcgtccg tgcgggacac cttttacatc atcggcacaa ccgccggacc
     5401 gcatccctac cccatgatgg tgcgcaattt tcagtcggtc atcgggcgcg aagtcaaaaa
     5461 gcagacggta aaagcggaag gccggaatcc cgatgttctg gtggcctgcg tgggcggcgg
     5521 atccaatgcc atgggcctgt tttatccgtt ccgccatgaa aagaaggtgg agatgttcgg
     5581 cgtggaagca gccggccgcg gcatgaacac gaacgagcat tcggcaagca tcaacggcgg
     5641 aaaaatcggg gtcctgcacg gcaacaaaac ctatctgctg caggatgaaa acggccaggt
     5701 gcgcgacgcc tattccatcg cggcgggact ggattacccc ggcgtgggcc cggagcacgc
     5761 ctatttcgcc caatccggcc gggccgccta tgtcaccgtg acggatgagg aagcgatcgg
     5821 ggcgttttta ttgttgtcgc ggcaggaagg gatcattccg gcgctggaaa gttcgcacgc
     5881 gatcgcccac gccatgaaaa tcgctccgaa aatgaagaag agtcagattc tggtcgtctg
     5941 tctttccggc cggggagaca aagacgcccg gacgattatg gaaacgctgc agggataaaa
     6001 tcctccccgg ccctccttac agaaaaggag ggcgtgatga tggactcgta aaaagtcctc
     6061 aatgccgtca ccccggcgaa aaccggggtc cggaaataat tgactatact ggattccgtt
     6121 tttcaccgga atgactactt tacttgcttt tcgacttttt acgggaccct ccgttgtaaa
     6181 tgaaaaagaa acgagga
//
LOCUS       DAOSRM010000111         5965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_12175_length_5965_cov_97.325381, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000111 DAOSRM010000000
VERSION     DAOSRM010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12175_length_5965_cov_97.325381"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLA58_09280"
     CDS             <1..423
                     /locus_tag="PLA58_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR63024.1"
                     /translation="HIGRFRAEERRITVQALDLLAVFHLDASANEKAKNLPYGLQRRL
                     EMARALATEPKLLLLDEPAAGMNPNEILQLMDFIRWIRKTFSVTILLIEHQMRLVMGI
                     CERLVVLDFGATIAEGLPAEIQANPKVLEAYLGEEVAS"
     gene            420..1139
                     /locus_tag="PLA58_09285"
     CDS             420..1139
                     /locus_tag="PLA58_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR63025.1"
                     /translation="MNRADLLLCVDGLSVHYDSIKAVDGISFEVARREIVTLIGANGA
                     GKSSILRAISGLVPYTGSVTFAGKDLRNLAADQVVAAGIAHVPEGRGIFGNLTVLENL
                     QLACWQRRNKPKIADDLSHVFELFPRLSERRKQLGGMLSGGEQQMLAVGRALMSDAQM
                     LLLDEPSMGLSPVLVREIFRIIQEINEAGKTILLVEQNANMSLHIASRAYVLETGHIV
                     LSGAGKELTGNPRVKEAYLGG"
     gene            complement(1451..3289)
                     /locus_tag="PLA58_09290"
     CDS             complement(1451..3289)
                     /locus_tag="PLA58_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOR63026.1"
                     /translation="MNTDQLFLRGSSLKAFIVFIAFQLVLIGAVFSAHRAQTDNGAVE
                     VSNVYFVNEDGNRIRGKLFRPLSATETVKHPGVLFIHGYQNNRESGDAYAIELGRRGF
                     VTLSIDAVGRGNSDPPHDMNDPRFDKTFGARTSAKYLASLPFVDSGRVGVMGHSMGAE
                     YSYWIALADPSIRAVAIIGNAYDKRATRDRPQNMIMIIGEFDEFRDRFTGTTNLMEQW
                     MKTEATLNAFPVANPQFNTTYGDFADGTARRVFVTRISHVQESHSRACIAEALDWMRL
                     ALHPPEQLWKDKNDQRWPIKEAMTLLAMLAGFLSLLPLGLIALRTRFFRPIRQGQTFG
                     YACPDGQWWKFAAINGALMWLYLPLIMTLFAIHKFVVRVDKVFPMLLVDAIVWWFVII
                     NIIGLILLRRWYRRESVAGGLSWYDLGISHFRERWGLDPGRTGRTVLLALLLFAFLYL
                     CEFLGEKLALVDYRFIYPLLSDLTPYRMLMFALYFPFILFGFLVLTSFVHGQLRRAEK
                     SSWWRTYVSWTGRNILALISPLFLFLLVQYVPLLTTGFIPFEGPGGVFVVFIIELFFI
                     MLTLTIVMIQSTWFYQISGTIYLSALMNALVVTWLFASSQVIAPIP"
     gene            complement(3455..3838)
                     /locus_tag="PLA58_09295"
     CDS             complement(3455..3838)
                     /locus_tag="PLA58_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63027.1"
                     /translation="MTAIHHNGRIDETGIMFPAADGFPLAGAFRTWRRWLHKGDYLMG
                     ELADRLKPNCCNDIRAPIAFCVFSADPLTTAESAQKLLTGMPNARTEFHVLRPSDLGV
                     RALGHQNLFRRKNEAAWPKIWDAAI"
     gene            4018..5217
                     /locus_tag="PLA58_09300"
     CDS             4018..5217
                     /locus_tag="PLA58_09300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR63028.1"
                     /translation="MNEKRLWTKDFVTIAVINAVVFLTHFLLIVTIASYAVDEFHAST
                     DMAGLVAGIFIIGALIGRLGTGRVIEGIGSKKVLIISTMLFIVTSALYFAAVSLSLLI
                     IIRLLHGIVFGTASTAAGTIVAQIIPPSRRGEGIGFYSIGAILAVAVGPFIGIFLIHH
                     ANFRMIFALTSFLAAASFVLSFAVNKPVYEKPAPESDAADGRFRLSDFLEFKAIPISL
                     IILIIGFSYSGLLTLISLYTKEIHLERAASFYFLVYSITVLITRPLSGRLMDIRGANF
                     VVYPSLLLYAVGMLIFSQATAGIALLAAAVFLAMGYGNFISSAQAISIRGVPPHRMGL
                     AIATFFIFLDLGFGIGPYLLGALVGFTGYRALYVIMTFVILASIPLYYVLLGRKENRL
                     RAHGQDS"
     gene            5271..5897
                     /locus_tag="PLA58_09305"
     CDS             5271..5897
                     /locus_tag="PLA58_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOR63029.1"
                     /translation="MENASPLVITISRQLGSGGGYIGQQLAKRLDIFYADREIISLAA
                     QHFSMLEKDLASRDEKKLSFWQSFIRTHAAAPDAYVKPQTLPPSDHELFKAESGIILR
                     IARERPAVIIGRCGAHVLRSHPNCVSIFLHADRAFRIDRVMKLYNTTEEAAGKIIDRS
                     DKERALYHRAVTGQEWADARRHHLSLATDKIGVDRCVECILSYLILRG"
BASE COUNT         1332 a         1717 c         1675 g         1241 t
ORIGIN      
        1 cacatcggcc gcttccgggc cgaagaaagg cgcatcaccg tgcaggcgct ggatctgctc
       61 gccgtgttcc atctggacgc atccgccaat gaaaaggcca aaaacctgcc ctatgggctg
      121 cagagacgtc tggagatggc ccgcgcgctc gccaccgaac ccaagctgct gcttctggac
      181 gaaccggccg ccgggatgaa ccccaacgaa atccttcagc tcatggactt tattcgctgg
      241 attcgcaaga cgttttcagt aaccatcctc ctgattgaac accagatgcg tctggtcatg
      301 ggaatttgcg aacgcctggt ggtcctggac ttcggcgcca ccatcgccga aggtctgcct
      361 gccgaaattc aggcgaatcc caaagtgctg gaagcctatc ttggcgagga ggtcgcttca
      421 tgaaccgcgc ggatcttctc ctttgcgtgg acggattgag cgtccactac gacagcatca
      481 aggccgttga cggtatttct tttgaggtgg cccgcagaga aatcgtcacg ctgatcggcg
      541 caaacggcgc gggcaagtcc tccatcctgc gcgcgatctc cggtctggtc ccctacaccg
      601 gaagtgtcac cttcgccggc aaagatttga gaaaccttgc cgccgaccag gttgtcgcag
      661 ccggcatcgc ccatgtgccg gaaggccgcg gcattttcgg caacctcacc gtactggaga
      721 atcttcagct tgcctgctgg cagcgcagga acaaacccaa aatcgccgac gatctgagcc
      781 atgtttttga actgttcccg cgtttgagcg aacgacgcaa acaactgggc ggcatgctgt
      841 ccggcggaga gcagcaaatg ctggccgtgg gccgcgcgct catgagcgac gcgcagatgc
      901 tgctgctcga tgaaccctcc atgggcctct cgccggtttt ggtgagagaa attttcagaa
      961 tcattcagga aatcaacgag gcgggaaaga cgattctact ggtcgaacaa aacgccaaca
     1021 tgtctttgca catcgcctcg cgcgcctacg ttctggaaac cggccacatc gtgctttccg
     1081 gcgccggcaa agaactgacc ggcaacccga gggtaaaaga agcctatctg ggcgggtaat
     1141 ttcgtgagag ctttgaataa cggccatttt tgtcatttcg accgaagaga gaaatcttga
     1201 acaaacggcc attatgaaga tttctcgtcg ctcacgctcc tcgaaatgac atttttcaaa
     1261 gctctcttcg tgtcgcgtca tgacgaaaag ctcagccagg gcggcatcgc ggatacctgg
     1321 aacagttgag ggatttgcac aacctgcttc cccgtcattc ctcgtgcccc aacctgtcat
     1381 tcccgcgcag gcggaaatcc aggttgatca accttttcca gactcccgcc ttcgagactg
     1441 tatcgcaatg tcacggaatc ggcgcgatca cctgtgacga tgcgaacagc caggtgacga
     1501 ccagcgcgtt catcagcgcg ctcaaataaa tcgttcccga aatctggtag aaccaggtgg
     1561 actggatcat cacaatcgtc agcgtcagca tgatgaaaaa aagctcgatg ataaacacca
     1621 cgaaaacgcc gcccggcccc tcgaacggaa tgaagccggt cgtcagcagc gggacgtact
     1681 gcaccagcag aaaaaggaag agcgggctga tcagcgccag aatgtttctc cccgtccacg
     1741 agacgtacgt tcgccaccag ctcgattttt cggcgcgtcg gagctgcccg tgcacaaacg
     1801 acgtcagcac caggaaaccg aaaaggataa acgggaaata gagcgcgaac atgagcatgc
     1861 gatagggagt cagatcgctc agcagcggat agataaaccg gtaatcgacg agcgccagct
     1921 tctcaccgag aaactcacag agatacagaa aggcaaaaag cagcagcgcc agcaggacgg
     1981 tacggccggt ccgccccgga tcgaggcccc atcgctccct gaaatgcgag ataccgaggt
     2041 cataccagct aagcccgccg gcgacggact ccctgcgata ccaccgccgc agcaggatga
     2101 ggccgatgat attgatgatg acgaaccacc agacgatcgc gtccacgagc agcatgggaa
     2161 aaaccttatc cacgcggaca acgaacttgt ggatcgcgaa gagcgtcatg atgagcggga
     2221 gatacagcca catgagcgcc ccgttgatcg cggcgaactt ccaccattgt ccgtcggggc
     2281 aggcataacc gaaggtctgg ccctgacgga tcggcctgaa gaaacgggtc cggagcgcga
     2341 tcagcccaag gggaaggagc gaaaggaaac cggccagcat agccagcagc gtcatcgcct
     2401 ctttgatggg ccaacgctgg tcgttcttgt ctttccacag ctgttccggc ggatgcaggg
     2461 ccaggcgcat ccagtcgagc gcctcggcga tacaagccct gctgtgcgac tcctgcacgt
     2521 gagagattcg cgtaacgaag acgcggcggg ccgtcccatc ggcaaaatcg ccgtaggtgg
     2581 tgttgaattg agggtttgcg accggaaacg cattgagcgt ggcctccgtt ttcatccact
     2641 gttccatgag attcgtggtg ccggtaaaac ggtcgcggaa ctcgtcgaac tcgccgatga
     2701 tcatgatcat gttctgcggc cggtcgcgcg tggcgcgttt gtcataggcg ttcccgatga
     2761 tggcgaccgc ccggatggag ggatccgcaa gcgcgatcca gtaggaatac tcggccccca
     2821 tgctgtgccc catcaccccg acgcggcctg aatccacaaa gggaagcgac gcaagatact
     2881 tcgccgaggt gcgggccccg aacgttttgt cgaaccgcgg atcgttcatg tcatgcggcg
     2941 ggtccgaatt gcccctgccg acggcgtcga tgctgagcgt tacaaagcct cttcgaccga
     3001 gctcgatggc gtaggcgtcg ccgctttcac ggttgttctg gtatccgtgg atgaagagta
     3061 cgccggggtg cttgaccgtt tcggtcgcgg agaggggcct gaagagcttg ccgcgaatcc
     3121 gattgccgtc ttcattcacg aaataaacat tggatacctc cacagccccg ttatccgtct
     3181 gcgccctgtg ggccgaaaat acggcgccga tgagcacaag ctggaaagcg atgaagacga
     3241 tgaaggcctt gagactgctc ccccgaagaa ataactgatc ggtattcatc ctgacctcct
     3301 tctcctggaa tcttgatctc agcaaaaaaa tttatccaaa attttaacgg ctaaaatcat
     3361 cgacatcggc cggaacctca ttttaccaaa ggcggcaagg gcaaagaaaa cagatcaagt
     3421 ctccgagcaa aagcctgaac gaagatgatc ctcgtcagat ggccgcgtcc cagattttcg
     3481 gccaggcggc ttcgtttttg cgccggaaca ggttctggtg gccgagcgcc ctgacgccga
     3541 ggtcggaggg ccgcagaaca tgaaactccg tccgggcgtt gggcatgccc gtcaggagtt
     3601 tttgcgcgct ttcggcggtg gtcagcggat cggcgctaaa aacgcaaaag gcgatcggcg
     3661 ctctgatgtc attgcagcag ttcggtttca gccggtcggc aagctcgccc ataagatagt
     3721 cgcccttgtg caaccaccgg cgccaggtgc gaaacgcgcc ggcaagcgga aatccatcgg
     3781 cggcgggaaa cattatcccc gtttcgtcga tcctcccgtt gtgatggatg gcggtcattg
     3841 aaacggattc tccaaatcgt caaaaggact tattttataa attgtacgcc atcttacaac
     3901 tttgaaaaaa caatgcaaga acaaaagcga ttgatccatt gttgttgaca ggcggaaatt
     3961 atgctcctat agtcccggac aaacacaaaa cgaaggcggt cggccgtgga acaggccatg
     4021 aacgaaaaaa gactgtggac aaaggatttt gtcacgatag ccgtgatcaa tgccgttgtc
     4081 tttctgacgc attttctgct gatcgtcacg attgcatcct atgccgtgga tgaattccac
     4141 gcatctacag acatggcggg gctggtcgcg ggcatcttca ttatcggcgc gctgatcgga
     4201 cggctgggaa ccgggcgcgt cattgagggc atcggcagca aaaaagtttt gatcatcagc
     4261 acgatgcttt tcattgtcac ttcggcgctg tacttcgcag ccgtcagcct gtctctgttg
     4321 atcatcatcc ggctcttgca cgggattgtc ttcgggacag ccagcacggc cgcgggaacc
     4381 attgtcgccc agattattcc cccaagccgc cgcggagaag gcatcgggtt ttacagcatc
     4441 ggcgcgatcc tggcggttgc ggtgggccct tttatcggca tttttctgat tcatcacgcg
     4501 aatttcagga tgatcttcgc gctgacatcg tttctggcgg cggccagttt tgtcctgtct
     4561 ttcgcggtca acaagccggt gtatgaaaag cccgccccgg aatccgacgc cgcggacggc
     4621 cgattccggc tttccgattt tctggaattt aaagcgatcc cgatttccct gatcatcctg
     4681 atcatcggct tcagctattc cggtctgctg acgctcatct ctctgtacac caaggaaatc
     4741 catctggaaa gggccgccag cttctatttt cttgtttatt ccatcacggt tctcatcaca
     4801 cggccgttgt ccggacgcct gatggacatc agaggcgcca attttgtcgt ctacccctcc
     4861 ctgctgcttt acgccgtcgg catgctgatc ttcagtcagg ccacggcggg aatagcgctg
     4921 ctcgcggcgg cggtctttct ggcgatgggc tacggaaact ttatttccag cgctcaggcc
     4981 atttcgatca ggggggttcc cccccatcga atggggcttg cgatagccac tttcttcatt
     5041 tttctggatc tgggatttgg aatcggccct tatttactgg gcgcgctggt tggcttcacg
     5101 ggataccggg ccttgtatgt catcatgact ttcgtcatcc tggcatccat tcccctgtac
     5161 tatgttctgc tgggaaggaa ggaaaaccgt ttgcgcgctc acgggcagga cagctgattc
     5221 cggacgggac cggcgcatcg ccgaagggtg aaagagacag gagaagccgc atggagaacg
     5281 catcgccttt ggtcattacc atcagtcgac agctcggctc cggtggcggt tatatcgggc
     5341 agcaactggc aaaacgcctg gacatttttt acgcggaccg cgaaatcatc agcctggcgg
     5401 cacaacattt ttcgatgctg gaaaaggatc tggcctcgcg cgatgaaaaa aagctctcct
     5461 tctggcagtc ctttatccgg acgcacgccg ccgccccgga cgcctacgtc aagccgcaga
     5521 cgctgccgcc ttcggaccat gagctgttca aggcggaaag cgggatcatc ctgcgcatcg
     5581 cccgggaacg tccggccgtg attatcggtc gctgtggcgc gcacgttctg cgaagccatc
     5641 caaattgcgt gagcatcttc ctgcatgccg accgggcgtt tcgcatagat cgagtgatga
     5701 aactgtacaa cacaacggaa gaagcggctg gaaagatcat cgaccggagc gataaagaac
     5761 gggctctcta ccatcgcgcc gtgaccggtc aggaatgggc cgatgcaaga cgccaccacc
     5821 tctctcttgc gacagacaaa atcggcgttg accggtgcgt ggaatgcatc ctgagctacc
     5881 tcatcttgag ggggtgatcg cgcgccgccg tagcggctct ttcccgagac tcaggcggca
     5941 gtcttgcggc aaacgtccgg ttttc
//
LOCUS       DAOSRM010000112         5922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_12295_length_5922_cov_133.415374, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000112 DAOSRM010000000
VERSION     DAOSRM010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12295_length_5922_cov_133.415374"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1848
                     /locus_tag="PLA58_09310"
     CDS             154..1848
                     /locus_tag="PLA58_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63030.1"
                     /translation="MQTAGISFLLGPYQNYVNNALDKMSRGNIAARIWANDFHVWKPS
                     PQEIANRLGWLHAPVETSAHVSEIRASVEPFTNGSIDDVVLLGMGGSSLSAEVFSNIF
                     GSAPGFPRLQILDTTDPVVIRRVARGLNLDKTLFIVSSKSGKTLEIRSLFHYFYNLAD
                     QTPGVSANEHFMFITDEGSPMLTQAWQLQLPYVFSNNPNIGGRYAALSLVGIIPAALI
                     GVSIEKLLQNAMAVANREKADFFSGRGGSTGFLLGAALGTLAQYGRDKLTLMLPPPWR
                     PFGDWLEQLLAESLGKEGKGVLPVLNEPPLDVDLYGNDRVFVVFYHDEDEWDSRISQL
                     AGAGHPVLKIRINGFYDLGGQMFLWEMATAVAAHIMGINPFDQPDVEATKTHTNRMIE
                     EFRENKGWPAPRPALSLTACEVYGDVSARTLPAALTKFLAQAGEGAYGGLQVYLSPSP
                     AIDDALQKLRLAISRRYKMAVTIGYGPRYLHSVGQLHKGDAGRGLFIQMTGPNKSDIG
                     IPDELGKPDSFLTFGQLKAAQAEADRRALLDKGRKIIRIHWKKDAAGGIRSLAKSL"
     gene            complement(1859..2434)
                     /locus_tag="PLA58_09315"
     CDS             complement(1859..2434)
                     /locus_tag="PLA58_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR63031.1"
                     /translation="MNYAELLQKRRAIRDFLDQEVPLPLVEEILRDATLAPSASNNQP
                     CRFVVVQCRKAIKNLSDESKACLLRDHNDGKIALNPTYVSLLADENFNVFYNAPSLIF
                     VAGPKSVGSLDVDAALAAGYIMFAAAARGLGTCWVALGANIRDPKLRGELGLSENLRI
                     VAPILLGYPTAIPAAPERHAPEILRVIAQAR"
     gene            complement(2458..3090)
                     /locus_tag="PLA58_09320"
     CDS             complement(2458..3090)
                     /locus_tag="PLA58_09320"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOR63032.1"
                     /translation="MGLIRLLISDPAAFVMLAVLLLYSVIFHELAHGWVACRMGDETA
                     KWLGRLTLNPLKHLDPIGSLMLLVIGFGWAKPVPINLENIPAPQRRKGLVLVSAAGVT
                     ANMLIAFFALLLWRLFSPEPGGMMYSVLYTLATINILLAAFNLIPIPPLDGSKILMGF
                     TPESVNRVLCQIEPFGFFIVLGLLLLGALNPVIAAFQYGIISFISLFLPG"
     gene            complement(3216..4382)
                     /gene="rpsA"
                     /locus_tag="PLA58_09325"
     CDS             complement(3216..4382)
                     /gene="rpsA"
                     /locus_tag="PLA58_09325"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOR63033.1"
                     /translation="MEDTKTFAEMFEESYAAPKRFAVGEKVEATIVKISPEWIFIDLG
                     AKSEGYIDKKEFLDEEGNLTAREGDSVTAYFLSSRHSERLFTTKLLSRKSVDEFLANA
                     YAHAIPLEATVEKEVKGGFSVKISASASGFCPFSQMELRRIGNAAEYIGKKFDFLVIE
                     YGENGRNIILSRRPLLEKIEQEKKSALKDSLKKGMTVKGVVASVRDFGAFVDIGGIQA
                     LLPVSEMTWGRVEDAKSLYKAGDTIEAVIINLDWENDRITLSFKDTLPDPWDDVVRNY
                     AEGSIHRGRVSRLTDFGAFVTLEAGVDGLLHISKLGRGKKIKHAQDVLAKDRDIDVKI
                     EKIDREHKKISLGLADNEDEKSGSREESGDDYKGYLPRSPKTMGTLGDLMKKKK"
     gene            4559..5860
                     /locus_tag="PLA58_09330"
     CDS             4559..5860
                     /locus_tag="PLA58_09330"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOR63034.1"
                     /translation="MRFEISRGGSGLTYEIRNIVMIAKKMQEYGLKIIWENIGDPVQK
                     GESIPDWMKEVLIDILRDDMSYAYSPTKGVDATRAFLAERTNARGKVQITSDDIIFFN
                     GLGDAIARAYSSIQVDARMIMPEPTYSTHLMAEVLHASFPPNTYRMNPYNNWAPDLRE
                     LEHKVRSHRAIVGILIINPDNPTGFVYPVEALENVVRIARENDLFVIADETYINIVYN
                     GRKTVPIGDVIGDVPGIAMKGISKELPWPGARCGWMEVYNADKDPIFARFINTILHQK
                     MSEVCSTTLPQMAIPRIMTHPEYKSYLRQRTQHYEKLSAIAYGVLKDVPFVIANKSNG
                     AFYMTVLFNESVLNERQHLHVEQPELLSYIETLTKGVEYDKRFVYYLLAATGICVVPL
                     TSFFTPLLGFRMTLLDKNVDEFEFVVKTLAEKITEYIQSSK"
BASE COUNT         1258 a         1705 c         1698 g         1261 t
ORIGIN      
        1 tatgtcgggc tttcgggaaa gatcatcggc atggagacct tcggcgcgtc cgcgcccgga
       61 ccggtgctgt atgagaagtt cggtttcacg tccgacggca tttttgcggc ggccagggaa
      121 ttactgacca ttcgctgaag aaggagcaaa ggggtgcaga ccgccgggat ttctttttta
      181 ctggggccct accagaacta cgtcaataac gcgctggata aaatgagccg cggcaatatt
      241 gccgcgcgca tctgggccaa cgactttcat gtctggaagc cgtcgccgca ggagattgcc
      301 aaccggctgg gatggctgca cgcgcccgtg gagacgtccg cccacgtcag cgagatccgc
      361 gcctccgtgg agcccttcac caacggcagc atcgacgacg ttgtccttct gggcatgggc
      421 gggtcgagcc tgtcggcgga agtgttcagc aacatcttcg gcagcgcgcc gggcttcccg
      481 aggctgcaga ttctggacac caccgatccg gtggtgatcc ggcgcgtggc acgcgggctc
      541 aacctggaca aaaccctttt catcgtttcc tccaagtcgg gcaagacgct ggagatccgt
      601 tcgctttttc attattttta caacctggcc gaccaaacgc cgggggtttc ggccaacgag
      661 catttcatgt tcattaccga cgaaggcagc ccgatgctga cccaggcctg gcagctacag
      721 cttccgtacg ttttttccaa caaccccaac atcggcggac gctacgcggc tctgtcgctg
      781 gtgggcatca ttcccgcggc gctgatcggc gtttccattg aaaaactgct ccagaacgcg
      841 atggccgtcg ccaaccggga gaaggcggat ttcttttccg gccgcggcgg ctccaccggg
      901 tttttgctgg gcgccgccct gggcacgctg gcgcagtacg gccgcgacaa actcaccctg
      961 atgctcccgc cgccctggag gcctttcggc gactggctgg agcagcttct tgcggaaagc
     1021 ctcggcaagg aaggcaaggg agtcctgccg gttttgaatg aaccgccgct ggatgtggac
     1081 ctgtatggga acgaccgcgt tttcgtcgtg ttttatcatg acgaggatga gtgggattca
     1141 cggatttcgc aattggcggg cgccgggcat cccgtcctca aaatccggat caacggcttt
     1201 tacgatctgg gcggccagat gtttttgtgg gaaatggcga cggccgtcgc cgcccacatc
     1261 atgggaatca atcccttcga ccagccggat gtcgaagcga ccaaaacgca cacgaaccgg
     1321 atgattgaag agttcaggga aaacaaagga tggccggcgc ccaggcctgc tctgtcgctc
     1381 accgcctgtg aggtttacgg cgatgtttcc gcccggacgc ttccggctgc gcttacgaaa
     1441 tttctcgcgc aggccgggga gggcgcgtac gggggattgc aggtttactt aagcccgtca
     1501 cccgccatcg acgatgcgct gcagaaactg cgcctggcga tttcccgccg gtacaaaatg
     1561 gccgtcacga tcggctacgg cccccgctac ctgcattcgg taggccagct gcacaaaggc
     1621 gacgccggcc gaggcctgtt tatccaaatg accggcccga acaaatcgga catcggcatc
     1681 cccgacgagt tgggcaagcc cgactccttc ctgaccttcg ggcagctcaa ggccgcgcag
     1741 gcagaagcgg accgccgggc gcttctggac aaagggagga aaatcatccg catccactgg
     1801 aaaaaagacg cggcgggcgg gatcaggtct ctggccaaaa gtctttagtc tttgtcgctc
     1861 accgggcttg tgcgatcacg cggagaattt ccggcgcgtg tcgttcgggc gcggcgggaa
     1921 tcgccgtagg atagcccaga agaatcggcg cgacaatccg caggttttcg gaaagcccca
     1981 gctcgccccg gagcttcgga tcacggatat tcgcccccag ggcgacccag caggtgccca
     2041 gcccccgggc ggccgccgcg aacatgatat agccggccgc gagcgccgcg tccacgtcca
     2101 gagagcccac cgatttcggc cccgcgacaa aaatcaggga gggcgcgttg tagaacacat
     2161 tgaaattttc atccgccagc aggctcacgt acgtgggatt caaggcaatc ttcccgtcgt
     2221 tgtgatcccg caggaggcag gccttgctct catcggaaag attcttgatg gccttgcggc
     2281 attggaccac gacaaagcgg cagggttgat tgttgctggc ggacggcgcc agtgtcgcat
     2341 cccgaaggat ttcctcgaca agaggcaggg gaacctcctg atccaggaag tcccggatgg
     2401 cccgcctctt ttgtaaaagc tcggcataat tcatcagggt gttcctcctt tctttgccta
     2461 tcccggcaga aacagcgata tgaacgaaat gatgccgtat tgaaacgcgg caatcaccgg
     2521 attcagcgct cccagaagca gcagacccag cacgataaaa aatccgaagg gctcgatttg
     2581 gcacagcacg cggttcaccg attccggggt gaatcccatc agaattttag agccgtccag
     2641 gggcgggatc ggaatcagat tgaacgcggc gagcaggatg ttgatagtcg ccagcgtata
     2701 gaggaccgaa tacatcatgc cgcccggttc gggggaaaaa agccgccaca gcaaaagcgc
     2761 gaaaaaagcg atgagcatgt tggccgtcac gccggcggcc gacaccagaa caagtccctt
     2821 gcgcctttgc ggcgcgggaa tgttttccag attgatcggg accggtttgg cccagccgaa
     2881 gccgatgacg agcagcatga gcgacccgat gggatccaga tgcttgaggg ggttgagcgt
     2941 cagtctcccc agccatttgg ccgtttcatc gcccatccgg caggcgaccc agccgtgcgc
     3001 cagctcgtgg aagatcacag agtacaaaag aagaacggcc agcatgacga aggcggccgg
     3061 atcgctgatc agcaggcgga ttaagcccat ccaacgctct ccatttctga cggcttctaa
     3121 aaagcccgga tgccgcgttg cgcagcatct tttcccggcc ttcgccgcgg ggcaggctta
     3181 gcgaaaagtc gagagaggga aaccttgtcc cttgtttact tctttttctt catcagatcg
     3241 cccagggtcc ccatggtttt gggcgaccgg ggcagatagc ctttgtaatc gtcgcccgat
     3301 tcttcccgcg agccgctttt ctcatcttcg ttgtctgcca ggccgagcga gattttcttg
     3361 tgttctcggt caatcttttc aatcttcacg tcgatgtcgc ggtctttggc gagcacgtcc
     3421 tgggcgtgtt tgatcttttt gccccggccc agtttggaaa tgtgcaaaag cccgtccacg
     3481 ccggcttcca gcgtgacgaa tgcgccgaaa tccgtcagcc gggaaacccg gcccctgtgg
     3541 atgcttcctt cggcgtagtt gcggacaacg tcgtcccacg gatcggggag cgtatccttg
     3601 aacgacagcg tgatgcggtc gttttcccag tccaggttga tgatgaccgc ctcaatcgtg
     3661 tcgcccgcct tgtacaggga tttcgcgtcc tccactcttc cccaggtcat ttccgaaacc
     3721 ggcagaagcg cctggatgcc gccgatgtca acaaatgcgc cgaaatcgcg cacggaggcg
     3781 accaccccct tcacggtcat gccttttttc agggagtcct tcagcgcgga tttcttttcc
     3841 tgctcgattt tttccagaag cggccgccgc gacaggatga tgttgcggcc gttttcgccg
     3901 tattcgatca cgaggaagtc gaattttttg ccgatgtact cggcggcgtt gccgattctt
     3961 ctgagttcca tctgcgaaaa ggggcagaag ccgctcgcgc tcgcgctgat ttttaccgaa
     4021 aatcctccct tgacctcttt ttcaacggtg gcttccagcg gaatcgcatg ggcgtaggcg
     4081 ttggccagaa actcatctac gctctttcgg gaaagcagct tggtcgtgaa aagtctttca
     4141 ctgtggcgcg aagaaaggaa ataggcggtc acgctgtcgc cttcccgggc ggtcagattg
     4201 ccctcctcgt cgaggaattc ctttttgtcg atatagccct cgcttttggc gcccagatcg
     4261 atgaagatcc attccgggga aattttgacg atggtcgcct ccactttttc acccacggca
     4321 aatcgcttgg gggcggcgta ggattcctcg aacatttcag caaacgtttt tgtatcttcc
     4381 atggaaaaag tcctttttgt gttttgatgt gcgggcctca atatcatgaa acgtttcgtt
     4441 tgtccttcta aaattgccgt ctgttccgca aaacagcacg aaaactaaaa aaatctggat
     4501 aaatccaaaa atatgtatta tgcattgttc tacaaatatc taaggagtta agcaacttat
     4561 gcgctttgaa atttcccgcg gcgggagcgg attgacgtat gagatccgca atatcgtgat
     4621 gatcgcgaaa aaaatgcagg aatacgggct gaaaatcatc tgggaaaaca tcggcgaccc
     4681 ggtccaaaag ggggaaagca ttcccgactg gatgaaagaa gtcctcatcg acatcctcag
     4741 ggatgatatg tcctacgcgt attcgccgac caaaggcgtg gacgccacgc gcgcttttct
     4801 ggcggagcgc accaacgcgc ggggaaaggt tcagattact tccgatgaca tcatcttctt
     4861 caacggcctg ggcgacgcga ttgcccgcgc ctacagttcc attcaggtgg acgcccgcat
     4921 gatcatgccg gagccgacct actccacgca cctgatggcc gaagtgctgc acgcgtcgtt
     4981 tccgcccaac acgtaccgga tgaatcccta caacaactgg gcgccggact tgcgcgaact
     5041 ggagcacaag gtgcgcagcc accgggcgat cgtcggcatc ctgatcatca atcccgacaa
     5101 cccgaccggc tttgtgtatc ccgtcgaggc gctggaaaac gtcgtccgga ttgcccgcga
     5161 aaacgatctt ttcgtgattg ccgatgagac ctatatcaat attgtttaca acggcaggaa
     5221 aaccgtgccg atcggcgacg tgatcggcga cgtgccgggc atcgccatga agggcatttc
     5281 caaggagctg ccctggccgg gcgcgcgctg cggctggatg gaggtgtata acgcggacaa
     5341 ggacccgatc ttcgcgcgct tcatcaatac gattctgcac cagaagatgt cggaggtctg
     5401 ctcgacgacg ctgccgcaga tggcgattcc ccgcatcatg acgcacccgg aatacaaaag
     5461 ctatctgcga cagcgaacgc agcattatga aaagctctcc gccatcgcct acggcgtttt
     5521 gaaggacgtc ccgtttgtca ttgccaacaa aagcaacggc gcgttttaca tgacggttct
     5581 tttcaacgag tccgtgctca atgaacgcca gcatcttcat gtcgagcagc cggaactgct
     5641 gagctatatc gagacgctga cgaaaggcgt cgagtacgac aagcggtttg tgtactatct
     5701 gctcgcggcc accggcatct gcgtggttcc gctgacgtcg ttcttcacgc cgctgctcgg
     5761 gttccgcatg acgcttctgg acaagaatgt ggacgaattt gaatttgtcg tcaaaacgct
     5821 ggcggaaaaa atcaccgagt atattcagtc gagcaagtag tggatggtgg atagtgaata
     5881 gcggatggta aaatgtaggg aacggtttca aaccgttccc ta
//
LOCUS       DAOSRM010000113         5760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_12713_length_5760_cov_120.365819, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000113 DAOSRM010000000
VERSION     DAOSRM010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12713_length_5760_cov_120.365819"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLA58_09335"
     CDS             complement(<1..649)
                     /locus_tag="PLA58_09335"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOR63035.1"
                     /translation="MPQEKILHYPNGAVKERKRLSDGKLEGEYTTYFPSGQIMAQLIF
                     RQGKRSGPAVSYYSNGQIREKATFINDKMDGLYVSYFESGRVREEEPYVNGKLHGAYR
                     RYFKTGELREQELFQNGKYEGEYVCYYKSGQIKEKGFFKNDKRHGDYVAYFKNGNVRE
                     KAVYKNGRLVGRFQTFREDGSPADEEAAEEEGARAWDEKDVAGLLHDLSHFDKKKK"
     gene            853..2046
                     /locus_tag="PLA58_09340"
     CDS             853..2046
                     /locus_tag="PLA58_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63036.1"
                     /translation="MIAADLDTTRVAIIGPGRLGTSFAYKLARDNKRVAVYYHNSDVC
                     KAINRDHLNPIHLTEDLALRMGGMDQVPRLGPKVYATNDLEHIVENNDFILLCVTMSR
                     LPELLNYLKPMIDRKAGDTCLISPIKGLIADEITSELITPSQLIHNHLYNLKHKCQVV
                     CIGGPFFDVDIALGKPVCLTVAGKRRTANMIRDHLLMFNRKELNAYYNFDMIGVEACG
                     ALKNIVANIKGLTDSLDLGDSIPGTLFARSGVEIRSLCRLLGGGFQAFQSQAGVGDMY
                     VTISSLASKNYRYGKYFYELHTGNPIETNLKVLGRIDGTPEGPNTIRNVYKYLEKKNM
                     YSPLFSCAYTIFNKGGNKNAIRDMIIHACQFDKRKNEYIGSFSRLMYRLIPDYWYRRD
                     KEFFE"
     gene            2059..3894
                     /locus_tag="PLA58_09345"
     CDS             2059..3894
                     /locus_tag="PLA58_09345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR63037.1"
                     /translation="MWKQKIVAPADVLEKIDPGMSIFLGTGMAEPRTLVRNLMESKQY
                     NLQDLELIQLVSLGDAVAIDERYSRKFRLKTFYSGWLASDAISQGRVDLIPSRFSRVA
                     WLFKTGAIHIDVAFIQVTPPDENGYAYLVGASVERQAMEMAQIVVGEINSQIPRTMGD
                     TLVNMADFHYLVEATEPPYYLARWPLQDVYLKIATNIASMIPDGSCISYGIGPLYEAL
                     AKSLTAKKNLGVHSPFFTDALMDLVKSGAVTNRYKNIFRGKSCASYVLGTRELMSWLD
                     LNPLVEFQPQDIVMDPRTIGLNDNMIAIFPARKVDLTGGVALHTGKGNVTAGPGNVAE
                     LFVGAALSRNGRSIFALPSRNRKGKPNILLSLDQYPFQFTNRESMDIVVTEYGVALLM
                     GKTLRERAQALIEIAHPDDRAELVRQAKEEKMIYADQIYFPESGYLYPEKVACSHRFR
                     DSLTVRFRAIKPSDEEEMRRLFYRFSDQAVYYRYFSPIKTMPHRKMQEYVNVDYRNTM
                     SIVAIVDESGVEKIIGEARYVKTQGEPFADTAFIVDEQYQGMGISTYLFNLLIRAARE
                     EGIAGFKADVLENNRAMLKVYEKALYPVQTVLSGGVYKITIPFNE"
     gene            complement(3934..4893)
                     /locus_tag="PLA58_09350"
     CDS             complement(3934..4893)
                     /locus_tag="PLA58_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOR63038.1"
                     /translation="MGISVFVTKDFAQLSEVAAAIAVRKTIGILKNKNEAVLGLATGN
                     SPTGLYKHFARAANEGKLDAGRIRSFNLDEYVGLPGENAQQRLLHPESYAYFMIQELF
                     GRLQKKFIETRVPAGSLIDQKILIRELRENKNDWEFRGTDAGKSIVIKPRPKSAYLAW
                     IKKDILDGYERKIKAAGGIDLQIIGVGGRGHVAFHESGIPFAGSRVLLVKLDDNTIAN
                     AVADGHFKTKAQSPQYAVSMGAELVYRAKTVLLLANGARKTESVARSLLGEVTPDIPI
                     SYGQTYAAQGGELIYVLDRVAAEGLAADRGALKKRGVILKNLV"
     gene            complement(4915..>5760)
                     /locus_tag="PLA58_09355"
     CDS             complement(4915..>5760)
                     /locus_tag="PLA58_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOR63039.1"
                     /translation="PRNLSMEGMKIVLDTANGATYKVAPDTFHELGANIEVIHKTPNG
                     TNINDGCGSQHTEILRKKVVENGAALGLAFDGDGDRLIAVDEKGREITGDQIMVICAN
                     ILKREGKLKNDLLVTTVMSNLGLTVACKKYGFRHHASKVGDRYVLEDMKRLDGIIGGE
                     EAGHMIFLDHHTTGDGIIAALQLVAAMIKEGKPLSELARMMDIFPQKLINVDVKRKPD
                     IEDVPKLVEAIRQAESELGGEGRVLVRYSGTQNMCRVMVEGPSDAVTEKYCRQIADVV
                     KSELG"
BASE COUNT         1165 a         1649 c         1603 g         1343 t
ORIGIN      
        1 attttttctt tttgtcgaaa tgactcagat cgtgcagcaa gcctgcaaca tccttttcgt
       61 cccaggcgcg cgccccttcc tcttccgccg cctcctcatc cgcgggagag ccgtcctcgc
      121 gaaaggtctg aaaccggccg accagacggc cgtttttgta aacggccttc tccctgacgt
      181 ttccgttttt gaagtaggca acgtagtcgc cgtgacgctt gtcgttttta aaaaacccct
      241 tttccttgat ttgtccgctt ttgtaatagc agacatattc cccttcgtac tttccgtttt
      301 gaaaaagttc ctgctctctc agctcgccgg ttttgaaata gcgccggtag gcgccgtgca
      361 gtttgccgtt tacgtaaggc tcttcttccc gcacgcggcc gctttcgaaa taggagacgt
      421 acaggccgtc catcttgtcg ttgataaacg tcgctttttc ccggatctgc ccgttggaat
      481 aataactcac ggcggggccg cttctcttgc cctggcgaaa aataagctgc gccatgatct
      541 ggccgctcgg gaaataggtc gtatattcgc cttccagttt tccgtccgaa agccgcttcc
      601 tttccttgac ggcgccgttg gggtaatgaa gtattttttc ctggggcata aagttgctca
      661 tgtcagccgt gttcggccgt tttctttctg ttttccggtt tttaaacaat cctccgggat
      721 aatgcaacta aaaacagccg cgctgggggc agattttgtt ttactttttt ttaaaagatg
      781 attagggtgt ggcgccagga cggtttgttt tatgtttgaa gtccatgatt taccattaaa
      841 gagcggacac caatgatcgc agccgatttg gataccacca gagtcgccat tatcgggccc
      901 ggacgcctgg gcacgtcctt tgcctacaag ctggcgcgcg acaacaagcg cgttgccgta
      961 tattaccaca attcggatgt ctgcaaggcc atcaaccggg atcacctcaa tcccattcat
     1021 ctgaccgagg atctggcctt gcggatgggc ggtatggacc aggttccccg tctgggaccg
     1081 aaagtttacg cgaccaacga cctggagcac atcgtcgaga acaatgattt catcctgctg
     1141 tgcgtgacga tgagccgcct gccggagctg ctcaattatc tcaagccgat gatcgaccgg
     1201 aaagcgggcg atacctgtct gatctcaccg atcaagggat tgatcgccga tgaaatcacg
     1261 tcggagctga tcacgccgtc gcagctgatt cataatcatc tgtacaacct gaagcataaa
     1321 tgccaggtgg tctgcatcgg cggtcctttt tttgacgtgg acatcgcgct gggcaagccg
     1381 gtttgcctga ccgtcgcggg gaagcggcgg acggccaaca tgatccgcga ccacctgctg
     1441 atgttcaacc gcaaggagct caatgcctat tacaacttcg acatgatcgg cgtggaagcc
     1501 tgcggcgcgc tgaaaaacat cgtggccaac atcaagggcc tgacggattc gctggatctg
     1561 ggggactcga ttccggggac cctcttcgcc cgatcaggcg tggaaatccg gtcgctctgc
     1621 cgcctgctgg gcggcggttt tcaggccttt cagtcccagg cgggcgtcgg cgatatgtat
     1681 gtcaccattt cctccctggc cagcaaaaac taccgctacg ggaaatattt ctatgagctg
     1741 cataccggca accccattga aaccaatttg aaagtgctgg gccggatcga cggcacgccg
     1801 gaaggcccca atacgattcg caacgtctat aaatatctgg aaaagaaaaa catgtacagc
     1861 cctctgtttt cctgcgccta tacgattttc aacaagggcg gcaacaagaa cgcgatccgg
     1921 gacatgatca ttcatgcctg ccagtttgac aaaagaaaaa atgaatatat cggatccttt
     1981 tcccgcctga tgtaccgtct gatcccggac tactggtacc ggcgggacaa agaatttttt
     2041 gaatgagggt aaacgagtat gtggaaacaa aaaatcgtcg cccccgcgga tgtgctggag
     2101 aaaattgatc cgggaatgag cattttcctg ggaacgggaa tggccgaacc ccgcacgctg
     2161 gtcaggaatc tgatggaatc caaacagtat aacctgcagg acctggagct gattcaactg
     2221 gtcagtctgg gcgacgcggt ggcgatcgac gagcgctact cgcggaaatt ccgcctcaaa
     2281 acgttttatt ccggctggct tgcctcggac gccatcagcc agggtcgggt ggatctgatt
     2341 cccagccgct tctcccgggt ggcctggctg ttcaaaaccg gcgccattca tattgacgtg
     2401 gcgttcattc aggtgacgcc ccccgatgaa aacggctacg cctatctggt gggcgcgtcc
     2461 gtggagcggc aggccatgga aatggcccag atcgtcgtcg gggagatcaa ttcgcagatt
     2521 ccccggacga tgggggacac gctggtgaac atggcggatt tccattacct ggtcgaggcg
     2581 accgaaccgc cctattacct cgcccgctgg ccgctgcagg acgtgtatct caaaatcgca
     2641 accaacattg cctccatgat tcccgacgga agctgcatct cttacggcat cgggccgttg
     2701 tatgaggcgc tggccaagag cctgaccgcg aaaaaaaatc tgggcgtgca ttcgcccttc
     2761 tttaccgacg cgctgatgga cctggtgaaa agcggcgccg tgaccaaccg ctacaaaaac
     2821 atcttccgcg gcaagtcctg cgcctcctat gtactgggca ccagggagct gatgtcctgg
     2881 ctggatttga atccgctcgt ggaatttcag ccgcaggaca ttgtgatgga cccgcgcacc
     2941 atcggtctca acgacaatat gattgcgatt ttcccggcga ggaaagtgga cctgaccggc
     3001 ggcgtcgcgc tgcacaccgg caagggcaat gtgaccgctg gccccggcaa cgtcgcggag
     3061 ctgtttgtgg gcgcggcgct ttccagaaac ggccggagca tctttgctct gcccagtcgc
     3121 aaccgaaagg gaaaacccaa tatcctgctt tcgctggatc agtatccctt ccagtttacg
     3181 aaccgcgaat cgatggacat cgtcgtcacg gaatacggcg tggcgctgct catgggaaaa
     3241 accctgcgtg aacgggcgca ggcgctcatc gagattgccc atccggacga ccgcgccgaa
     3301 ctggtccgcc aggccaagga agaaaagatg atctacgccg accagattta ttttcccgaa
     3361 tccggctatc tgtatcccga aaaggtcgcc tgctcacacc gcttcaggga ttcgctgacc
     3421 gtccgcttcc gggcgatcaa gccgtccgat gaagaggaaa tgcggcgcct gttctaccgg
     3481 ttttccgatc aggcggtgta ttaccggtat ttcagcccca tcaaaaccat gccgcacagg
     3541 aagatgcagg aatacgtgaa tgtcgattac cggaacacga tgtccatcgt ggcgattgtg
     3601 gatgaatccg gcgtggaaaa gattatcggg gaggcccgct acgtcaagac gcagggcgag
     3661 ccttttgccg acacggcgtt cattgtggat gagcagtacc aggggatggg catttcaacg
     3721 tatctgttca acctgttgat ccgcgcggcc cgcgaagagg gcatcgccgg attcaaggcc
     3781 gatgttctgg aaaacaaccg ggccatgctg aaagtttacg aaaaggcgct gtatccggtg
     3841 cagaccgttt tgtcgggcgg cgtctataaa attacgatcc ccttcaacga atagcgaaag
     3901 gacgccttca ggcgaaggct ttccaccggt ccgctacaca aggtttttca aaatcacgcc
     3961 ccgttttttg agcgctcccc ggtcggccgc aaggccttcc gccgcgacgc ggtcgagcac
     4021 ataaatcagt tctccgccct gggcggcgta cgtctgcccg taggagatgg gaatgtccgg
     4081 ggtgacttcg cccaggagcg accgggctac ggactccgtt ttgcgcgcgc cgttggccaa
     4141 aagcagaacg gtttttgccc ggtacaccag ctcggccccc atgctgacgg cgtactgcgg
     4201 ggactgagct ttggttttga aatgcccgtc ggcgacggca ttggcgatgg tgttgtcgtc
     4261 gagcttgacc agcagaaccc ggctgcccgc aaagggaatg cccgattcgt ggaaagcgac
     4321 gtggccgcgg ccgcccacgc cgatgatctg caggtcgatg ccgcccgccg ctttgatttt
     4381 ccgctcatag ccgtccagaa tatctttttt gatccaggcg aggtaggccg acttcggacg
     4441 gggtttgatg acaatggatt tccccgcgtc cgttcccctg aattcccagt cgtttttatt
     4501 ttcccgaagt tcccggatca ggattttctg gtcaatcaaa gaacctgccg gaacgcgggt
     4561 ttcgatgaat ttcttctgca ggcggccgaa gagctcctgg atcatgaaat acgcgtagct
     4621 ttccgggtga aggagccgct gctgggcgtt ctcacccggc aggccgacgt attcgtccag
     4681 gttgaagctg cggatgcggc cggcgtccag ctttccttca ttggccgccc gggcgaagtg
     4741 cttgtagagc cccgtggggg agtttcccgt ggccaggccc aacaccgcct cgtttttgtt
     4801 cttcaggatg ccgatggttt tccggacggc aatggccgcc gccacctcgc tcaactgcgc
     4861 aaagtctttg gttacgaaga cgctgatgcc catgatgagt tttccccttt cgtcctaccc
     4921 cagttcgctt ttgaccacat cggcaatctg acggcagtat ttttccgtca ccgcgtcgga
     4981 cggtccctcc accatgacgc ggcacatatt ctgcgttccc gaatagcgga ccagcacgcg
     5041 cccttcgccg cccagttcgc tttccgcctg ccggatggct tcgacgagct tcggcacgtc
     5101 ttcgatgtcc ggtttgcgct tcacgtccac attgatcagt ttctgcggaa agatatccat
     5161 catccgcgcg agttcggaaa gaggctttcc ttccttgatc atcgccgcca ccagttgcag
     5221 ggcggcaatg atgccgtctc ccgtcgtgtg atggtccagg aaaatcatgt gacccgcctc
     5281 ttcccccccg atgatgccgt cgagcctttt catgtcttcc agaacgtagc ggtcgccgac
     5341 tttggaggca tggtggcgga agccgtattt tttgcaggcc acggtcaggc cgaggttgct
     5401 catgacggtg gtgaccagca ggtcgttttt cagtttgcct tcccgcttca ggatattggc
     5461 gcagatgacc atgatctggt cgcccgtgat ttcccggccc ttctcatcca cggcaatgag
     5521 acggtcgccg tctccgtcaa aggccaggcc cagggccgcg ccgttctcga ccactttttt
     5581 ccggaggatt tccgtatgct gcgagccgca gccgtcattg atgttggttc cgttgggcgt
     5641 cttgtgaatg acttcgatgt tggcgcccag ttcatggaag gtgtcggggg cgaccttgta
     5701 cgtggcgccg ttggcggtgt ccagaacgat tttcatgcct tccatggaaa gattgcgggg
//
LOCUS       DAOSRM010000114         5712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_12847_length_5712_cov_100.071946, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000114 DAOSRM010000000
VERSION     DAOSRM010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12847_length_5712_cov_100.071946"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PLA58_09360"
     CDS             complement(<1..1226)
                     /locus_tag="PLA58_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOR63040.1"
                     /translation="MGKIFKTGCVLCAQNCGLEIEVENNRIVKVRGDKSNAKSEGYIC
                     RKGLNVAYHQHNADRLKYPLKKVRDKFERISWDQAIDEIATKLKGIIDEYGPRSFAYM
                     GGGGQGCHFEAAFGVRLMRSLGSQYQYSALAQEFSGAFWVCGRTHGKQYLHDQPDVDN
                     TDVLLIFGWNGMQSHQIPQAPRKLQRLSKDKDKMLIVVDPRKTETAKLADIHLPIRPG
                     TDALLLKAMISLILKEGWENKAYLADHTSGFDSVKSCFENFDARAAVKTCSLDFEQVR
                     EVTRLYATRKSSLRYDLGLFMGRHSALNSYLIVILQAICGRLCSPGGNVINGHLMPIG
                     PHTDERDSKIWRTMATNSFPVCGSFPPNVMPEEILSDHPERLRAVIVTQSNPLRSYAD
                     TTAYEKAFQRLDLLVTG"
     gene            complement(1270..1695)
                     /locus_tag="PLA58_09365"
     CDS             complement(1270..1695)
                     /locus_tag="PLA58_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOR63041.1"
                     /translation="MKKNDCIFFQFAKTYQLSSRFLAQKVSELNLTSVQAMVLGFLTE
                     EDQITSRELGKRTELDSATLTGILDRLEAAEFLERKSNPDDRRSIHIHLTPKGKAMGR
                     EASRVIVEANAEFLQVLTEEQKRDLHSIISTLRLNVPRP"
     gene            1800..2018
                     /locus_tag="PLA58_09370"
     CDS             1800..2018
                     /locus_tag="PLA58_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63042.1"
                     /translation="MGEGVADDLRIFDESDDTHDALTFWTDQGIDLVYFLNQPRPAIS
                     GRKRLFERRVFSPCNNSSEDLAFRHEQK"
     assembly_gap    2147..2246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2247..2473
                     /locus_tag="PLA58_09375"
     CDS             <2247..2473
                     /locus_tag="PLA58_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOR63043.1"
                     /translation="WDKVEDLPWVPQVLVERLKHYFSTYKLIPGQEKSIWIENLYGRD
                     RALEVVNAAMEDYLDLLGKSVGQPGHKEPL"
     gene            2470..3522
                     /locus_tag="PLA58_09380"
     CDS             2470..3522
                     /locus_tag="PLA58_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR63044.1"
                     /translation="MKTALQFIVLSFTALVFLSGSGVQSAHASEDPIRVAALLDLSGH
                     YSQFGLETKQAMELGLSETKKIDVRFFDTKGDLKTAEQKLNEVIQQGGYTVVVTLASW
                     ISNGLAAKIKSNHLLQMAVGSAVFDHADQKNCVRFTGDVGREIQYLTNYLQSYNKVAV
                     MYFNNDYGISWNNALKSTLGSKLVKSIAYTDTDVDFTHPLNEVKAVHPDALALISTRE
                     AVQIAKQASKIRIQSRMFGTRPTLTAPLLAEPTARGLMFSYPDLNETLPIFASFKRKY
                     GYRMSAFGAEGYDLIKSLDRFCRHGKKNKVLFANYQNRTYDGALGRITFNENAQADYD
                     YTICVLQNGTWGKMKR"
     gene            complement(3575..5113)
                     /locus_tag="PLA58_09385"
     CDS             complement(3575..5113)
                     /locus_tag="PLA58_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOR63045.1"
                     /translation="MQTFFISSMHSLKYFLPAALLVTLLTSCAPQYFSPVSGDAFTRR
                     DLQALSGRELWHGFVFNGEKVGFARLKIEPEAGGQSYTLFSEASLRIRFLGMGKSVSI
                     RGEDRVAPDLSLLSFRYEQTIDEKTLALEGGIRNGLLSVVQRSGNEVKTSETKISSPL
                     YPSSGINLYPVLRGMKTGADYAYPVYDPQTQSIVDVAQSVVAFEESRRLGIEPSFKLE
                     TRMSGHVAESWINLQGETVFERGMGGVLITFKESERGAKSFLAEASMGKKDLIYDFSL
                     IRTDTPITCPRKADFLEATVEVPEKTLSPLQSPHQHISPQQQDKETAWIYRIHKIAPD
                     VPDAPRQALNAKDRFLFLAPFHHIESDHPEILEAARKAVAGAQTTRGKVERLTSWVAN
                     EIKDEPVDSFSALEVLHNRKGECQAHTLLYTAMARALGIPTRLAGGIVYMEGMGFLYH
                     AWAESFVDDWLPVDATFNQVPVDATHIKLVEGPDWISLLPMGSVIGHIRIRNIRFHCG
                     ENGN"
     gene            complement(5176..>5712)
                     /locus_tag="PLA58_09390"
     CDS             complement(5176..>5712)
                     /locus_tag="PLA58_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR63046.1"
                     /translation="LTKFSLSGGLTLCCPEGGRAVLETFVNAPFTVALEALPYPANVR
                     ELPEEAASLPLPVEALPLVHSGLTLGYRLEIGAKVIAYCPDTGYCQNAVTLARGADLL
                     ITECAYKEGQSSESWPHLNPETAGRIAREAGALRLAMTHFDARIYRSLPERMEAQTAA
                     RKEFPAAFAACDDMILEC"
BASE COUNT         1253 a         1469 c         1591 g         1299 t
ORIGIN      
        1 ccggtcacca gaaggtccag gcgctgaaat gccttttcgt acgccgtcgt gtccgcatac
       61 gagcgcagcg ggttggattg ggtaacgatc acggctctca gccgttcggg atgatccgac
      121 agaatttctt ccggcatgac attgggcgga aaggaaccgc agaccggaaa ggaattggtg
      181 gccatggtgc gccagatttt ggaatctctt tcatccgtat gcggcccgat gggcatcagg
      241 tggccgttga tcacattgcc cccgggagaa cagagcctgc cgcagatcgc ctggagaatc
      301 acgatcaggt aggaattcag agcgctgtgg cgtcccataa aaaggcccag atcgtagcgc
      361 agcgacgatt tgcgcgtcgc gtaaaggcgg gtgacttcac ggacctgctc gaagtccaga
      421 gagcaggtct ttacggcggc gcgagcgtcg aagttttcga aacaggattt caccgaatcg
      481 aagcccgacg tatggtcggc caggtacgcc ttgttttccc agccttcctt cagaatcagg
      541 gaaatcatgg ctttgagcag cagggcgtcc gtgccggggc ggatgggcag atggatgtcg
      601 gcgagttttg ccgtttcggt tttacgcgga tcgaccacga tcagcatttt gtctttgtct
      661 ttggacagac gctgaagctt gcgcggggcc tgcggaatct gatggctctg catgccgttc
      721 cagccgaata tcaaaagaac atcggtgtta tcgacatccg gctgatcatg caggtactgc
      781 ttgccgtgcg ttcgcccgca aacccagaag gcgccggaaa attcctgcgc cagcgcgctg
      841 tactggtatt gagagcccag gctccgcatc agacgcacgc cgaaagccgc ttcaaaatga
      901 cagccctgtc cgccgccgcc catgtaggca aacgagcggg ggccgtattc gtcaatgatg
      961 cccttgagct tcgttgcgat ttcatcgatg gcctggtccc aggagatccg ctcgaatttg
     1021 tccctgactt ttttgagcgg atatttcagg cggtcggcgt tgtgctggtg ataggcgacg
     1081 ttcagaccct tgcggcagat atacccttcg cttttggcgt tggatttgtc gcctcgaact
     1141 ttgacgatgc ggttgttttc cacttcgatt tccagaccgc agttctgcgc gcagaggaca
     1201 cagccggttt taaatatttt tcccatgttt gatcctgttc tccggcggat tcaccggggc
     1261 ttgcgcgtct taggggcgcg ggacgttaag acgcagggtt gaaataatac tgtgaagatc
     1321 acgcttttgt tcttccgtca ggacctgcag gaactcggca ttggcttcca caatcacccg
     1381 gctcgcttcc cggcccatgg ccttgccttt cggggtcaga tggatatgga tggaccggcg
     1441 gtcgtctgga ttgcttttgc gttcgagaaa ttccgcggct tccagccgat ccagaatgcc
     1501 cgtgagcgtg gcgctgtcga gctccgttcg tttgcccagt tccctggagg tgatctggtc
     1561 ttcttccgtc agaaatccca aaaccatggc ctgaacggac gtgaggttga gttccgaaac
     1621 cttctgggcg agaaaccggc tggagagctg ataggtcttg gcgaattgaa agaagatgca
     1681 atcgtttttc ttcataggat gcaaaatccg tctcgcaggc aatttacttg tatgcaagca
     1741 taatcgatca caaaaattct gtcaaatcat tttatgcggg ggcttgacag gagtcccaga
     1801 tgggtgaggg tgtcgcggat gacctgcgca tcttcgatga atctgatgat acgcatgatg
     1861 ccttgacatt ttggacagac caggggatcg acctcgtata ttttctgaat cagccgcgcc
     1921 cggccatttc agggcggaaa agactttttg agaggcgtgt tttttcccct tgcaataatt
     1981 ccagtgagga tttagccttc cgccatgaac aaaaatgatt ctttttcccg ctggcggccc
     2041 catccatggc atgggcttga ggtgggcccc catccgccga acattgtaca tgcttacatc
     2101 gagatcaccc cgtttgacac ggtcaagtac gaggtggaca aactctnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnntctg ggacaaggtg gaagacttgc cctgggtgcc
     2281 ccaagttctt gtggaacgcc tgaagcacta cttcagcacc tacaaattaa ttccgggaca
     2341 ggaaaagtcc atttggattg aaaacctcta cggcagagac cgcgcgctgg aagtcgtgaa
     2401 tgccgccatg gaggattatc tggatctgct ggggaaaagc gtcggacaac ccggccataa
     2461 ggagccgtta tgaaaacagc cctccaattt atcgtgttga gttttactgc cctggtcttt
     2521 ctgtcgggca gcggtgtgca gtcagcccac gcatcagaag atcccataag agttgcggcc
     2581 ttattggatt tatcgggaca ttacagccaa tttggtctgg aaaccaaaca ggcgatggag
     2641 ctgggcttat cggaaaccaa aaagatcgat gttcggtttt ttgatacgaa aggtgattta
     2701 aaaacagcag aacaaaaatt gaacgaagtc atccagcagg gtggttatac cgtggtggtt
     2761 acgctggcaa gctggatcag caacggcctt gccgcaaaga tcaaatcaaa ccatctgttg
     2821 caaatggcgg tgggcagcgc cgtctttgat catgccgatc agaaaaactg tgtgaggttc
     2881 accggtgacg tcggcagaga aattcaatac ctgaccaatt acttgcaaag ctacaacaaa
     2941 gttgccgtca tgtatttcaa taacgattac ggcatttcct ggaacaatgc cttaaagtcg
     3001 acattgggaa gcaaactcgt caagtctatc gcttacaccg ataccgatgt ggattttacg
     3061 catccgttga atgaagtaaa ggcggttcat ccggatgcgc ttgctttaat cagcacaagg
     3121 gaagccgttc aaattgccaa acaggcatcc aaaatccgga ttcagtcccg gatgtttggg
     3181 acaaggccga cattaaccgc tccattgctt gctgagccaa cggcaagggg gttaatgttt
     3241 tcctatccgg accttaatga aactctcccg attttcgcgt cttttaagag gaaatacgga
     3301 tacagaatgt ctgcttttgg cgctgaagga tacgatttga tcaagtccct ggatcggttt
     3361 tgccgtcatg ggaagaagaa caaagtgctt ttcgcgaatt atcaaaacag aacgtatgat
     3421 ggcgctttgg ggcgtattac atttaatgaa aatgctcagg cagattacga ctataccatc
     3481 tgtgtcctcc aaaacggaac ctggggaaag atgaagcgat aacctcttcg acgttcagat
     3541 ctcttcgcct tcacacgggg aaggacagcg tttgtcaatt cccgttttca ccacaatgga
     3601 accggatgtt tctgattcgg atgtgtccga tgacgctgcc catgggcagg agggatatcc
     3661 agtcggggcc ctccaccagc ttgatatggg ttgcgtccac gggaacctgg ttgaaggtcg
     3721 catccacggg aagccagtcg tccacaaagc tttccgccca cgcgtgatac aaaaacccca
     3781 tgccttccat atagacaatg ccgccggcca gccgcgtggg aattcccagc gcccgcgcca
     3841 tggccgtgta aagcagggtg tgggcctggc attctccctt ccggttgtga aggacctcca
     3901 gcgccgagaa agaatccacc ggctcatcct tgatttcatt cgccacccag gaggttaacc
     3961 gttcgacttt cccgcgggtc gtttgcgcgc ctgcgaccgc tttccgggcc gcctcgagaa
     4021 tttccggatg atcgctttcg atgtggtgga aaggggcgag gaaaaggaag cgatcttttg
     4081 cgttgagcgc ttgacggggc gcatccggaa catcgggggc tattttgtgg atgcggtaga
     4141 tccaagccgt ctctttgtcc tgctgctgcg gggaaatatg ctgatggggg ctttgcagag
     4201 gagagagggt tttttccggg acctcaacgg tggcctccag aaaatccgct tttcggggac
     4261 aggtgatggg ggtgtccgtc cggatgaggc tgaagtcata aatcaaatcc tttttgccca
     4321 tgcttgcttc ggccagaaag ctcttcgcgc ctctttcgct ttccttgaac gtgatcagta
     4381 cgcctcccat gccgcgctcg aagaccgtct ctccttgcag gttgatccag ctttccgcga
     4441 cgtgccccga cattcgcgtt tccagtttga aggagggctc aatcccgagc cgacggcttt
     4501 cttcaaaggc aacgaccgac tgggctacat ccacgatcga ctgcgtctgc ggatcataga
     4561 ccggataggc ataatcggcg ccggtcttca ttccccggag aaccggataa aggttgattc
     4621 cgctcgaggg atacagtgga gatgaaatct tcgtttcgga ggtcttgacc tcgttcccgg
     4681 accgttgaac aaccgaaagg agaccgttgc ggatgccgcc ttcgagggcc agcgtttttt
     4741 cgtcaatcgt ctgttcatat cggaaagaga gcagagaaag atcgggggcc acccggtcct
     4801 cgccccgaat gctgacgctc tttcccattc cgagaaaacg gatgcgcaga ctggcttcgg
     4861 aaaacagcgt ataagactgg ccgccggctt ccggttctat cttcaggcgg gcaaacccca
     4921 ctttctcgcc gttgaaaaca aagccgtgcc agagctctcg gccggacagc gcctgcagat
     4981 ccctgcgcgt gaaagcatcg ccggagacgg gtgaaaaata ttgcggggcg caggacgtca
     5041 gaagcgtcac aagcagagcg gccggcaaaa aatatttcag cgagtgcatg gaggaaatga
     5101 agaaagtttg cacgaggcga agacattttt ccggagctga tttgatcaaa ggatgaagat
     5161 ccatcgcgga caccatcagc attcaagaat catatcgtca caggcggcaa aggcggcggg
     5221 gaattccttc cgggccgctg tttgcgcctc cattctttcc ggaaggctcc ggtagatgcg
     5281 ggcgtcaaag tgggtcatcg ccagacgcag agctccggct tcccgcgcga tccggccggc
     5341 cgtttcggga ttgaggtgcg gccagctttc ggaggactgc ccctctttgt aggcgcattc
     5401 cgtgatcagc aggtccgcgc cccgcgccag cgtcacggcg ttttggcagt accccgtatc
     5461 cgggcagtag gcgatgactt ttgcgccgat ctccagacga taaccgagcg tcagccccga
     5521 atggacgagc ggcagggcct caacgggcaa ggggagtgac gccgcttctt cgggcagttc
     5581 acgcacgttt gccggatagg gcagggcctc cagcgcgacg gtgaaaggcg cgtttacgaa
     5641 tgtttcaaga acagcccggc cgccttccgg acagcagagc gtcaatccgc cggaaagcga
     5701 aaatttggtc ag
//
LOCUS       DAOSRM010000115         5633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_13085_length_5633_cov_93.533345, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000115 DAOSRM010000000
VERSION     DAOSRM010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13085_length_5633_cov_93.533345"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(461..1168)
                     /locus_tag="PLA58_09395"
     CDS             complement(461..1168)
                     /locus_tag="PLA58_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOR63047.1"
                     /translation="MDRLRYTKCTSLFDGQPSAVDRQQRLEQPLKRINRNPKSSPTSF
                     EESSPGREEIRFKVFQKRIAQTAEKTGGIVDEKYIAIVAAALERMDETVQRLAKMRDV
                     RKVVDILAVAQPLQQAMRMVAHAWMHLWSMTAAIPKLKALTGDLALEAVVKDNTEAAF
                     YCGKILSDQCYIGTELKHLHGYLDHIMTGEPAVVNGCSEEIFTGALPQKTGGGHTAGP
                     LWFPVHGNAPVSGCAQG"
     gene            1167..2108
                     /locus_tag="PLA58_09400"
     CDS             1167..2108
                     /locus_tag="PLA58_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63048.1"
                     /translation="MILFQHPPRRWRALFLLTFALVIVSGVDSAAFDFPAAGWHRGDA
                     AFAQENSRAALQKALASANPNIELDIIDFVDARGRRVGLVSHDYLMKRATGLDGTFGE
                     KYKDPSQLPQNAANPLLPPQPFMTVAELFDLIRETKARGVTPIVSLDMKEEGKNAEAF
                     ARWVGERIREYGFQDHIFASSFFLNNIKPLKAACPECLAGGLVFNDHWGLRFLSPHYA
                     SVDITPLSKVTFFLGFLGKKEYPLDFILLQDDIFFENPDLVNYWKNKRKVKFVGVFVY
                     NKDRPYTEDEWALLKKADWLELDPPQMNQYLQRKSGK"
     gene            2240..2377
                     /gene="rpmH"
                     /locus_tag="PLA58_09405"
     CDS             2240..2377
                     /gene="rpmH"
                     /locus_tag="PLA58_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOR63049.1"
                     /translation="MKKNLTNKSNTKRKRTHGFLVRMATKTGRQVLSRRRAKGRKRLT
                     V"
     gene            2773..3000
                     /gene="yidD"
                     /locus_tag="PLA58_09410"
     CDS             2773..3000
                     /gene="yidD"
                     /locus_tag="PLA58_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOR63050.1"
                     /translation="MFRKTLVNFFVFLIRLYQACISPFFFAPCCRFSPSCSQFAIDAI
                     RLHGPAKGIFLGAKRILRCHPLHPGGYDPVK"
     gene            3024..4712
                     /gene="yidC"
                     /locus_tag="PLA58_09415"
     CDS             3024..4712
                     /gene="yidC"
                     /locus_tag="PLA58_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOR63051.1"
                     /translation="MDKRTILAIVLSLAVLLVYQIFFVKPPVPSKGTVPQASTGQVRQ
                     DADAPSTPAPAATTASARPALKSAVGPQAPPRDITVETAHYTAVFSTRGAALKSLKLK
                     DYYRDCHECTDDIWPKIKGLVTGTKAPLKPKTPEFVELVDVREGMPYPLAVTFPESGA
                     DVAPDEVFEAAQSKLDLTGSREKQQLVFSKSFHNVKIEKIFSFDPSGYTIGMDVKVYN
                     LTGTPITQIPQLNWYQYVDPQKIDDSYGHEGPIVSVGGSVELQEVKKLQGQSIQGPNV
                     LWGAFESKYFIASFIPENPSLTNAVMTRDEADMVAVGLKGRKEIIPGGQNSVFSYSLY
                     LGPKQYDLLKHLGVGLENAIDFGWFKWLAIPALYILNFLYGFVLNYGIAIIILTTIIK
                     IIFWPLGNMSYKSMNEMKKLQPKIEALKQKYKNDQTKIGQETMALYREHKVNPFSGCL
                     PMLIQIPVFFGLYKALMYSIELRHSPFFFWIQDLSAKDPYYITPIIMGATQFITQKMT
                     PAMGDPMQQKIMLLMPVIFTFFFLNFPSGLVIYWLWNNILQIGQQYYINKKLAT"
     gene            4733..>5633
                     /gene="jag"
                     /locus_tag="PLA58_09420"
     CDS             4733..>5633
                     /gene="jag"
                     /locus_tag="PLA58_09420"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOR63052.1"
                     /translation="MSAKTLDIEEKTIDVAIERACREFGVGREKLNIEIISEGSNGFL
                     GIGAKKAKIRASLFSFDMDFSLPEAARTTRQAAPPEEKSDGKTERKADRPPDKKPEAR
                     HEQRQERQREAKPEMRHKSEHRAQRPASAESGASAPRPAPTRVKPAEPQEKSPSPAAA
                     AVEAAPATTPAALKARDLLTGMLGRMTFECQINATETDETIILSIAGDDSGLLIGRRG
                     QNLDALQYILNKSINKSETERKMIVVDSEEYRKRREESLLDMAERIREKVKKTQKPLS
                     LSHMNAHDRRIIHLALQEDDELIT"
BASE COUNT         1433 a         1571 c         1364 g         1265 t
ORIGIN      
        1 tgtgttaggc acgccgccag cgttagttct gagccaggat caaactctcc agttttaatc
       61 ctgtaaagga agataaatct tccttatgaa ctcgcttgat acatcaatca attaatagga
      121 cccacaaatt tcttttccca ctattcaatt tccaaagagc catcttgaaa cgagctgtga
      181 cacataaatc aatttgattt tcctgtcaag cttaaatttc aattttgttc tgtcaggaaa
      241 caggaatcat ccgacaacga agattgacac catcggtaaa catgttgaaa agatcaactc
      301 accttcaagc acatctctga cgaacagaag tggacttcta ctccctataa acaaaactgt
      361 caagaagaaa ttgcattatt ttccgattat ttttccattt cctgttagct tcatatttac
      421 agtcacttac gactttaccc ttcggccgaa ccttaaaaaa ctacccctgc gcgcatccgc
      481 ttacaggggc atttccatgc accgggaacc acaaaggccc tgcggtgtga ccgccgccgg
      541 tcttctgggg cagcgccccg gtaaagatct cttccgaaca gccgttcacg acagccggct
      601 ctcccgtcat gatgtggtcc agatagccat gaaggtgctt taactccgtc ccgatgtagc
      661 actgatccga aaggattttc ccacagtaga aggccgcttc ggtattgtct tttacgacgg
      721 cttccagcgc gagatcgcct gtcagcgcct tgagtttggg aatggccgcg gtcatggacc
      781 acaggtgcat ccaggcatgg gcgaccatcc gcatggcctg ctgcagcggc tgggccaccg
      841 ccagaatgtc gacgaccttc cggacatccc gcattttggc gagcctctga accgtttcgt
      901 ccattctttc cagagcggcc gctacgatgg cgatgtattt ttcatcaacg attccgccgg
      961 ttttctcggc cgtttgcgca atgcgctttt ggaaaacctt gaatcgtatt tcttcccttc
     1021 caggagaaga ttcttcgaag cttgtcgggg aggacttcgg atttctattg attcttttta
     1081 aaggctgttc taagcgttgt tgccgatcca cagcagacgg ctgcccgtca aataacgacg
     1141 tacattttgt ataacggagg cgatccatga tcctttttca acatccgccc cgccgatggc
     1201 gcgccctttt ccttctgacc tttgctcttg tcattgtttc gggcgtcgac agcgccgcat
     1261 tcgacttccc cgcagcgggg tggcaccggg gcgatgcggc ctttgcacag gaaaactcgc
     1321 gtgcagcgct gcagaaagcg ctggcttccg ccaatcccaa catcgagctg gacattattg
     1381 actttgtcga tgcccggggc agacgggtcg gactggtttc tcacgattac ctcatgaagc
     1441 gggccaccgg ccttgacggc acattcggcg aaaaatacaa ggacccttcc cagctgccgc
     1501 aaaatgcggc gaatcccctc cttccgcctc agccgtttat gacggttgcg gagctgtttg
     1561 atctcatccg cgaaacgaaa gcccggggcg tcacaccgat cgtctctctg gacatgaagg
     1621 aagaggggaa aaacgctgaa gcattcgccc gatgggtggg agaaagaatc cgggagtacg
     1681 gttttcagga ccacattttc gcatcgtcat tctttctcaa caatatcaag cctctcaaag
     1741 ccgcctgccc cgaatgcctg gcgggaggcc tggtcttcaa tgaccactgg ggacttcggt
     1801 ttctcagccc tcattacgcc tctgtggaca ttaccccttt gagcaaagtc actttctttc
     1861 tgggattttt gggaaagaag gaatatcccc tcgatttcat cctccttcag gacgacattt
     1921 tctttgagaa tcccgatctg gtcaattact ggaaaaacaa gcgcaaagtg aaatttgtgg
     1981 gtgttttcgt gtacaacaaa gaccgtccct acactgagga cgagtgggcg cttctgaaaa
     2041 aagccgattg gctggagctg gatccgccgc agatgaatca gtatctgcaa agaaaatccg
     2101 gaaaataact tcctgtcgcg ggctgcgcaa caccgggcgg ccaacgagcg gaaaagccgc
     2161 gggaacaggt ccactttgac cttgacaaat gcaggtgact gtggtttagc gaatgcccta
     2221 ttacttttag gggtttaaca tgaaaaagaa tttaacgaat aaatccaaca ccaagagaaa
     2281 aaggacccac ggatttctgg tccgcatggc aaccaaaacc ggccggcagg tgctgagccg
     2341 cagaagagcc aaaggcagaa agagattaac cgtttaattc cgctattgaa cgtctcgaag
     2401 ggatgttggg tttcttcagt aatgaaaaca caatctttcc gaagagcgga aaaggtgacc
     2461 agtaaaagca gttatcggac catttacgaa cagggggtca gaagaagttc gcgattcttt
     2521 actctcataa cctgcggcaa cccgaacgga gccagacgcc tgggaattac cgtcacgaag
     2581 aaaacgggca acgccgtttt tcgcaaccga atgaaaagac ttcttcggga attcttccgc
     2641 cgcaacaaag acttgtttcc agcgggacat gacgtcgtcg tcatggtcag aaaaaatata
     2701 ccgcctctca cctattcgga aacagccagg gaattgaccg aactcttctt gcggaaagac
     2761 ggcaaataaa gcatgttcag gaaaaccttg gtcaatttct ttgtttttct cattcgtctg
     2821 taccaagcct gtatttctcc attctttttt gcgccctgct gccgcttttc cccgtcctgt
     2881 tcgcaattcg caattgacgc gatcagactg cacggaccgg cgaaaggaat ctttctggga
     2941 gcgaagagaa ttctgcgctg ccatcccctt catcccggcg gttacgatcc cgtcaagtaa
     3001 acaagaaatt gggaggtatg ttcatggaca aaaggactat tctggccatc gtcctttcac
     3061 tggccgttct tttggtgtat caaatctttt ttgtcaagcc ccccgtgccg tcaaaaggca
     3121 ctgtgccgca ggcatccacc gggcaggttc ggcaggatgc ggacgctcct tcgactcccg
     3181 caccggcggc gaccacggct tcggcccggc ccgcgctgaa atcggcagtt gggccacagg
     3241 cgcctccccg cgacatcacg gtggaaaccg ctcattatac ggctgttttc tccacacgcg
     3301 gcgcggctct gaaatccctg aagctcaaag actactatcg ggattgccat gaatgcaccg
     3361 atgatatctg gccgaaaatc aaagggctgg tgaccggtac aaaagccccc ctcaagccca
     3421 aaacgccgga gtttgtggag ctggtcgatg ttcgcgaagg catgccctat ccgctggcgg
     3481 tgaccttccc cgaatccggc gccgatgtcg ctccggatga agtttttgaa gccgcccaaa
     3541 gcaaattgga tttgaccggg tcccgggaaa agcagcagct tgtgttttcc aaatccttcc
     3601 acaacgtcaa aatcgaaaag attttttcgt ttgatccttc aggctacacc atcggtatgg
     3661 atgtcaaggt ttacaatctc accggaacgc ccatcacgca gattccgcag ctgaactggt
     3721 accagtatgt tgatccccag aaaatcgatg acagctacgg acatgaaggc cccattgtct
     3781 cggtcggcgg cagcgtcgaa ctgcaggaag tcaaaaagct ccagggccag tccattcagg
     3841 gcccgaacgt gttgtgggga gcgtttgaaa gcaaatattt catcgcgtct ttcatcccgg
     3901 aaaatccgtc tctgaccaac gccgtcatga cgcgcgacga ggccgacatg gtcgccgtcg
     3961 gcctgaaggg ccgcaaggaa atcattcccg gcgggcaaaa cagcgttttt tcctactcgc
     4021 tgtatctcgg ccccaaacag tatgacctgt tgaagcatct cggcgtcgga ctcgaaaacg
     4081 ccattgattt cggatggttt aaatggctgg ccatcccggc tctttacatt cttaactttc
     4141 tgtacggatt tgtcctcaat tacggcattg ccatcatcat cctgaccacg atcatcaaaa
     4201 tcatcttctg gccgctgggc aatatgagct acaaatccat gaacgagatg aaaaaactcc
     4261 agccgaaaat cgaggccctg aagcaaaaat acaaaaatga tcagaccaaa atcggccagg
     4321 aaacgatggc gctttaccgg gaacacaagg tcaatccctt ttccggctgt ctgcccatgc
     4381 tgatccagat ccccgttttc ttcggcctgt ataaagcgct gatgtattcc atagaactgc
     4441 gccactcgcc gtttttcttc tggattcagg atttgtcggc aaaagaccct tactacatca
     4501 cgccgatcat catgggcgcc acccagttca tcacccaaaa aatgacaccc gccatgggcg
     4561 atccgatgca gcagaaaatc atgctgctca tgcccgtcat tttcaccttc ttctttctga
     4621 atttcccgtc gggactcgtg atctactggc tgtggaacaa tattttacaa atcggtcaac
     4681 agtactacat caataaaaag ctggccacgt gacaggatct acggagttca acatgagcgc
     4741 aaagacactg gatatagaag aaaaaaccat tgatgttgcc attgagcggg cctgccggga
     4801 attcggcgtc gggcgcgaaa aactcaacat cgaaattatt tccgaagggt ccaatggatt
     4861 tctgggaatc ggcgcgaaaa aggcaaaaat ccgcgcctca ctcttttcct tcgacatgga
     4921 tttctctctc ccggaggccg cccggaccac ccggcaagcc gcaccgcccg aagaaaaatc
     4981 cgacggcaaa acggaaagga aagccgaccg tccaccggat aaaaaaccgg aagccaggca
     5041 cgaacagcga caggaaagac agcgcgaggc caaaccggaa atgcggcaca aatccgaaca
     5101 tcgggctcaa aggcccgcgt ccgcggagtc cggagcatcc gcaccccgcc cggcgccgac
     5161 ccgggtaaaa ccggcagaac ctcaggaaaa atcaccctcg cccgccgcgg ccgcggtcga
     5221 agcggcgccc gccacaacgc ccgcagcgct caaagccaga gaccttttaa ccggcatgct
     5281 cggcagaatg acttttgaat gccaaataaa cgccaccgaa acggatgaaa ccatcatcct
     5341 cagcatcgct ggagatgaca gcggtcttct gatcggccgc cgggggcaga atctggacgc
     5401 gcttcagtac atcctgaaca aatcgatcaa caaatcagaa acggaacgca agatgatcgt
     5461 ggtggattcc gaggagtatc gcaaacgaag agaggaatcc ctgctggaca tggccgaacg
     5521 gattcgggag aaagtgaaga aaacccagaa acctctttcg ctttcccaca tgaacgctca
     5581 cgaccggcgg atcatccatc tggcgctgca ggaagacgac gaactcatca cca
//
LOCUS       DAOSRM010000116         5597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_13205_length_5597_cov_95.019668, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000116 DAOSRM010000000
VERSION     DAOSRM010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13205_length_5597_cov_95.019668"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLA58_09425"
     CDS             <1..605
                     /locus_tag="PLA58_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOR63053.1"
                     /translation="YKNAVRAGFTSGPGLGEELFQQILDHPEGIWAGKIDPENNFAEV
                     KTEDGRIHLFIPELIDELNTVEAAREEAALRLPADFPLILMAGRHTDKNANTLMRDPA
                     WNEGKRACTLAMHPEDAAALNLKDTEQVRVITEAGTEEIELEVTDTAHKGHVVIPHGF
                     GMIYNGVKYGANVNRLTKNTHRDQFGTPIHRYVPCRVEAI"
     gene            622..1425
                     /locus_tag="PLA58_09430"
     CDS             622..1425
                     /locus_tag="PLA58_09430"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOR63054.1"
                     /translation="MGELVKISIADHIADVRFNRPEKYNALSFDMIDAMADAINRLAK
                     EPGVRVVVISGEGKSFCAGLDVENFAALLSGEKRFPHLTERYQNRITNIYQHVGYGWK
                     ELPVPVIAAIHGVALGGGFQIAMGADIRFSTPDAKFSLMEMRWGLIPDMSVTQTIRDF
                     VPMDVAKELIFTGKVIKGEEAARLNLVTHICEKPLEEAMALAREIASKSPDAVRAAKK
                     LINEVWHGDSARGLMMESELMMTLMGSPNQLEAVNANFTKQSPKFIDPV"
     gene            1442..2140
                     /locus_tag="PLA58_09435"
     CDS             1442..2140
                     /locus_tag="PLA58_09435"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOR63055.1"
                     /translation="MDLKKVNETLNTYIRPQTFPIAVKLFTAQEALPSRVKVPVRDLG
                     YPIALCQAWALSRRHGMVVAVGKEDQCCIGGHVAMGFMDNPTAGDASNRHEPGTYAYM
                     LSAPIDRTDFEPALIALYVNSAQAMRLAQAATNGGGGIGPTFATGMGSCGDMVARTVK
                     TDLSQFILPSGGNRVFASTQDDELIFTIPKSKIDLMMKALADTHAIGFRYPVLADVRH
                     RPNLPPFLEIPKNA"
     gene            2172..3200
                     /locus_tag="PLA58_09440"
     CDS             2172..3200
                     /locus_tag="PLA58_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOR63056.1"
                     /translation="MWNASKCDLCGDCLVRCRYVDYDRDRAAREIRLLMEGQAADILG
                     KCITCNACVQYCPTGADPADLIYALQEKTGASPIVAGFKPFIDAVIQGFEEGGPDRII
                     EGEAGKPVLSMDSFGIKQFPEGTLESRLFKGMTVVRGKRFASLVGMCHMGGASLAGRY
                     ARQVIEGLAALGKDIVYLHNEGYVLAHVQARELGIDVPYRYLHLFEYLRDYLRNHAKD
                     ITALNRKIAYQPNCAVRWIPHQDAWLDEIFDLIGVKRVSRKYEGVDALCCGGPALAVN
                     RDLATKIQNDNIRDAMDHQAEALVTICPMCNVVLGAPTAKAGLPKIFITDLCRMALGE
                     APWPATAN"
     gene            complement(3187..5214)
                     /locus_tag="PLA58_09445"
     CDS             complement(3187..5214)
                     /locus_tag="PLA58_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOR63057.1"
                     /translation="MTQESSSAKRRLLRDINLMGAEYYEKFYRQTYLEWKKFVSGKRN
                     IDRSIIPQQVFDLWINCAELGLDPLAYPENEVLTGDALVALLAEHREFIDVSRPFMKN
                     LYRFLEGSGFMVSLLNSKGYVLEIFGDPEEDKLVQTARGYAGACWDLKNTGNNAASAV
                     VFYKNPVQVFGAQHYVRMYHGATGCGSPVFSPEGQLLGCLALFGRYYRANPHTLGMTV
                     AAANAIENELRTRKALSETRVAYSYQQTVISSIQEALIAVDSAGHVTLMNDNARKVFG
                     FPEDADFEGRRLRDIFKRGNERFFDLIENQETVTDTEVRIFFKHLSGDFTLTCNPILS
                     SGKNSIGKVIILNEIQRAKSMVTRMMGATANFHFEDIQGKNPRFLTTVDQAKMVSRSR
                     SNVLLLGKSGTGKDIFAQAIHNESDRRNGPYVAINCGAIPRDLIASELFGHEEGAFTG
                     SRRGGNQGKFELADGGTLFLDEIAETPLELQTALLRVIEDKSVLRIGGTRVRPVNVRI
                     IFSTNKDLREEVRKGNFREDLYYRANVFAIEMIPLKDRLDDIPLLADCFIRRYAVAMK
                     KNISRVDEKVMEAFMRYTWPGNVRELQNAIERMVNFLQTPVLTADLIPDHIRRPDGAD
                     EFGADAEARQEADRETLAGLLNSKMPKNRIAQKMNISRATLYRKMKQFNLQ"
BASE COUNT         1301 a         1596 c         1529 g         1171 t
ORIGIN      
        1 tgtacaagaa tgccgttcgc gcgggcttca catccggtcc cggcctgggc gaagaattgt
       61 ttcagcagat tctcgatcat cccgaaggca tctgggcggg aaagatcgat ccggaaaaca
      121 actttgccga ggtcaaaacc gaagacggca gaatccatct gttcattccc gagctgatcg
      181 atgaactcaa caccgtcgaa gcggcgcggg aagaggcggc gctcaggctg cctgccgatt
      241 ttccgctcat cctgatggcg ggccgccaca ccgacaaaaa cgccaacaca ctcatgcgcg
      301 accccgcctg gaatgaaggc aaacgcgcct gcacgctggc catgcatccg gaagacgcgg
      361 ccgccttgaa tctgaaagac acagagcagg tgcgggtcat cacggaggcc ggcacggaag
      421 agatcgaact ggaagtcacg gataccgcgc acaaaggtca tgtggtgatt cctcacggat
      481 tcggaatgat ttacaatggc gtgaaatacg gcgccaacgt gaaccgcctg accaaaaaca
      541 cgcaccgcga ccagttcggc acgccgattc accggtatgt accgtgccgg gtggaggcaa
      601 tataaactga aaggagccaa tatgggtgaa ctggtaaaaa tttccatcgc agaccacatt
      661 gcggatgtcc gtttcaaccg gcccgaaaaa tacaacgcgc tcagctttga catgatcgac
      721 gccatggccg atgccatcaa ccggctggcc aaggagcccg gcgtccgggt cgtggtgatt
      781 tccggcgaag gcaagagctt ctgcgccggg ctggatgtcg aaaacttcgc ggctctcctg
      841 tccggagaaa aaaggtttcc ccatctgacg gagcgctatc agaatcggat cacgaacatc
      901 tatcagcatg tcggctacgg gtggaaagag cttcccgtgc cggtaatcgc agccattcac
      961 ggcgtagcgc tcggcggcgg gttccagatt gccatggggg cggatattcg cttcagcacg
     1021 cccgatgcca agttctcact gatggaaatg agatggggac tgattccgga tatgagcgtt
     1081 acccagacca tccgcgattt cgtgccgatg gatgtggcca aggaactgat ctttacaggc
     1141 aaggtgatca aaggcgaaga agcggcccgg ttgaatctgg tgacgcacat ctgcgagaag
     1201 ccccttgaag aggccatggc tctggcccgg gagattgcat ccaaaagtcc cgatgccgtg
     1261 cgcgcggcga aaaaactcat caatgaagtc tggcacggag attccgccag ggggctcatg
     1321 atggaatccg aactgatgat gacgcttatg ggaagtccca accagttgga ggcggtgaac
     1381 gcaaacttca ccaagcagtc gccaaaattc atagacccgg tttgaagaaa aggaggaagg
     1441 catggacctg aaaaaagtca atgaaacgtt gaacacgtac atccggccac agacttttcc
     1501 catcgccgtt aaacttttta cggctcagga agcgctcccg tccagggtta aggtccccgt
     1561 cagggatctg ggctacccca ttgcgctttg ccaggcctgg gctctctccc ggcgacacgg
     1621 catggttgtg gccgtcggca aggaagatca gtgctgcatc gggggccatg tcgccatggg
     1681 atttatggac aatcccacgg cgggagatgc cagcaacagg catgagccgg gaacatacgc
     1741 ctacatgctg tccgcgccca tcgaccggac cgatttcgag ccggcgttga ttgctcttta
     1801 tgtcaattcc gcccaggcca tgcgtctggc gcaggcggca acaaacggag gcggcggcat
     1861 cggaccgacg tttgcgaccg gcatgggcag ctgcggcgac atggtggcgc gcacggtcaa
     1921 aacggatctg agccagttta ttctcccctc gggcggaaac agagttttcg cgtccacaca
     1981 ggacgacgaa ctcattttca cgattccgaa aagcaaaatc gacctgatga tgaaggcgct
     2041 ggccgacaca cacgccatcg gattccgcta tcccgtcctg gcggatgtac ggcaccgtcc
     2101 gaacctgccg cccttcctcg aaattccgaa gaatgcgtga ccccgggaaa gaaaatcaat
     2161 gaggtgaaac catgtggaat gcttcaaaat gtgatttatg cggcgattgt ctggtcaggt
     2221 gccgctacgt cgattacgac agggacaggg ccgcgcgcga aatcaggctt ctcatggaag
     2281 gccaggcggc ggacattctg gggaaatgca ttacctgcaa cgcctgcgtt caatactgcc
     2341 cgaccggcgc cgatccggcc gatctcatct atgccctgca ggagaagacc ggcgccagcc
     2401 cgattgtcgc agggttcaag ccttttatag atgccgtcat tcaggggttt gaggaaggcg
     2461 gcccggaccg gatcatcgaa ggagaggcgg gcaaacccgt tttgtccatg gactccttcg
     2521 gcatcaaaca attcccggag ggaacgctgg aaagccggct gttcaaaggc atgaccgtag
     2581 tccggggaaa aagattcgct tccctggtcg gcatgtgcca catgggcggc gcgagccttg
     2641 ccggacggta tgcccgacaa gtgattgaag ggctggccgc gctgggcaaa gacatcgttt
     2701 accttcacaa cgaaggatac gttctggccc acgtccaggc aagggagctg ggcattgacg
     2761 tcccctaccg gtatctgcac cttttcgagt acctgcggga ctatctgaga aaccatgcaa
     2821 aagacatcac cgcgctgaac cggaaaatcg cctaccagcc caactgcgcc gtccggtgga
     2881 ttccccatca ggacgcctgg cttgatgaaa ttttcgatct catcggcgtg aagcgcgttt
     2941 cgagaaaata cgaaggagtg gacgccctct gctgcggcgg accggcgctg gccgtcaacc
     3001 gggatctggc gacaaagatc cagaacgaca acatccggga cgccatggac caccaggccg
     3061 aggccctggt caccatctgt cccatgtgca acgtcgtgct cggcgcaccg accgcaaaag
     3121 ccggtctgcc gaaaatattc atcaccgacc tgtgccgcat ggccctgggc gaggcgccct
     3181 ggcccgctac tgcaaattga actgcttcat tttccggtaa agggtcgcgc gggaaatgtt
     3241 catcttctga gcgatgcggt ttttgggcat tttcgaattc agcagtccgg ccagcgtctc
     3301 ccggtcggct tcctggcgcg cttcggcgtc cgcgccgaat tcatccgcgc cgtctggacg
     3361 gcggatatgg tccggaatga gatcggccgt cagcaccggc gtctggagaa aattaaccat
     3421 tcgttcgatg gcgttctgca actcccgcac gtttcccggc caggtgtagc gcataaacgc
     3481 ctccatcacc ttttcgtcca cgcgggaaat gttttttttc atcgcaacgg catagcgccg
     3541 gataaagcag tccgccagca gcggaatgtc gtcgagccgg tccttaagcg gaatcatctc
     3601 gatggcgaaa acgttggcgc gatagtagag atcctcgcga aaatttccct tgcggacctc
     3661 ttcccgcagg tccttgttgg ttgaaaaaat aatgcgcacg ttcaccggcc ggacgcgcgt
     3721 gccgccgatg cgcagcacgg atttgtcctc gatgacccgc aaaagcgcgg tttgcagctc
     3781 caacggggtt tcggcaattt catcgagaaa cagcgtgccg ccgtcggcca gctcgaattt
     3841 gccctgattg ccgcctcgcc gcgaccccgt gaaggcccct tcttcatgcc cgaacagttc
     3901 actggcaatc aaatcccgcg gaatggcgcc gcagttgatg gcgacgtagg ggccgtttct
     3961 ccggtcgctc tcgttgtgaa tggcctgggc aaaaatgtcc ttgcccgttc cgctctttcc
     4021 caagagcagg acattggagc ggctccggga aaccattttg gcctggtcca ccgtcgtcag
     4081 aaagcggggg tttttcccct gaatatcttc aaagtgaaaa ttggcggtcg ctcccatcat
     4141 ccgggtcacc atggacttgg cccgctgaat ttcattgaga atgatgacct tgccgatgct
     4201 gtttttgccg gacgacagaa tcgggttaca ggtcagcgta aaatcgccgg aaagatgttt
     4261 gaaaaaaatg cggacttccg tatcggtgac cgtttcctgg ttttcgatca gatcgaaaaa
     4321 ccgttcgttt ccccgtttaa aaatatcccg gagccgccgc ccttcaaagt cggcatcctc
     4381 cgggaagccg aacaccttcc gtgcattgtc gttcatcagc gtcacatggc ccgcggaatc
     4441 caccgcgatg agcgcctcct gaatcgatga aatcaccgtc tgctgatagc tgtaggccac
     4501 acgggtttca gacaaggctt ttcgggtgcg cagctcattt tcaatggcat ttgccgccgc
     4561 caccgtcatg cccagcgtgt gagggttggc gcggtaatac cggccgaaca gcgcgagaca
     4621 gcccagcagc tgtccttccg ggctgaaaac aggcgaaccg catcccgtcg cgccgtgata
     4681 catccggacg taatgctgcg cgccgaacac ctgcacggga tttttataaa aaacaacggc
     4741 gcttgcggca ttgttgcccg tgtttttcag gtcccagcag gccccggcat agcccctggc
     4801 cgtctgcacc agtttgtcct cttcgggatc gccgaaaatc tccagcacat accccttgct
     4861 gttcagaagg ctgaccatga atcccgatcc ctccagaaag cggtacaggt ttttcatgaa
     4921 cggacggctg acatcaatga attctctgtg ctccgcaagc agggccacca gcgcatcccc
     4981 ggtcaatact tcattctccg gataggctaa cggatcaagg ccaagttcag cgcagtttat
     5041 ccacaagtca aacacctgct gagggataat ggagcggtca atgttgcgtt tgcccgacac
     5101 aaattttttc cattccagat aggtctgccg gtaaaatttc tcatagtact cggcgcccat
     5161 cagattaata tcccgaagca gtcttcgttt tgccgaactg gactcttgcg tcattcctca
     5221 ctccttcctt tatcaaccgg actctgtctc acgaatgtct caagaatgtc tcaagaatgt
     5281 ctcaatgaga cacttgtaaa gcattttttg atacaaaaat ccagagaaaa ttaaaaaatt
     5341 taaagcaaaa caacatgata aaattgttta agaattggca tactgaatgc tagtcatttt
     5401 ttaaataatg attggatgaa ccaaatacaa tcaatgcggt taatttcact aaccgctaaa
     5461 atcccccttt tttaatccta ccgaaggggg agagggtttc ggggagtgaa caggtatctc
     5521 caacatgcct tccctggacc ctctccctca ccttccttga acgttcagac ttatttttaa
     5581 ggatcagccc atcgcct
//
LOCUS       DAOSRM010000117         5525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_13445_length_5525_cov_101.995612, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000117 DAOSRM010000000
VERSION     DAOSRM010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13445_length_5525_cov_101.995612"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..182
                     /locus_tag="PLA58_09450"
     CDS             24..182
                     /locus_tag="PLA58_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63058.1"
                     /translation="MTKMAVIQSSLFSFFPAIPVDKLPGLSYTAREKQKTNKGMAHGK
                     NHYKSLRG"
     gene            148..630
                     /locus_tag="PLA58_09455"
     CDS             148..630
                     /locus_tag="PLA58_09455"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOR63059.1"
                     /translation="MEKTITKAYVDDKGFVLLVCSNCNTIQQQEAKSYGGAKGPISIT
                     CGNCGNVYDVEIEFRKFYRKGIRVDGLYFGVSNPKIWGKMVLKNVSMQGCGFETMGAN
                     PFQPGDLIKIEFQLNDAKRSVIKKQAAVRSTFKKYVGCQFQDLPNAMDADLGFYLRTP
                     "
     gene            684..1394
                     /locus_tag="PLA58_09460"
     CDS             684..1394
                     /locus_tag="PLA58_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOR63060.1"
                     /translation="MNDVLAAIGLAPVLIAQALYVRRVTPRLPEPSGERSGVHGSGPL
                     LKLLIVGDSAAAGVGVPLQNQALSGQLARALGEDFRVSWKLIAQTGHRAKDVLAVLEE
                     TDPEKFDVVVTSIGVNDVTHGTGRQTWIARQEKIIRLLLSKFHSPFIVLSGIPPMHLF
                     PALPQPLRWYLGSRASQFNRALQKAVESCSRCRFVRIDFPLEASYMADDGFHPGARAY
                     AFWADHLADVIRRHKNQA"
     gene            complement(1416..2147)
                     /locus_tag="PLA58_09465"
     CDS             complement(1416..2147)
                     /locus_tag="PLA58_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOR63061.1"
                     /translation="MKYYKALFLLVFFLAGCATWAEKPLQVLTYDQPGGCRQGLVVFL
                     RGMGSGAESFEEEGFVDEVRARGLAYDMAAPDAHLGYYIDRSMIARLKEDVIEPAKMR
                     GCRKIWLIGVSMGGLGAILYLKEHPQDVAGIYLMAPFLGKKFILNEIEAAGGVRLWNP
                     GVYQDEDDWERMLWHWIKTAVGDHPDKMIYLGYGTDDFYRQGSQLLAEVLPPDRVYAI
                     PGGHDYPTFKALWKLFLSGSAPLKE"
     gene            complement(2144..2569)
                     /locus_tag="PLA58_09470"
     CDS             complement(2144..2569)
                     /locus_tag="PLA58_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_6728 family protein"
                     /protein_id="HOR63062.1"
                     /translation="MQTFLPYPDFAQSLRVLDSRRLGKQRLEAMQLLRAISADGSGWS
                     RHPAAAMWRGYTNALKQYHNLAMDEWVRRGYLNRMPKQVIRGKITYPLWLGDESFHAS
                     HRSNLLRKDREYYGAFGWLEPDDLPYVWPVEPVRKSRKT"
     gene            complement(2640..3836)
                     /gene="dinB"
                     /locus_tag="PLA58_09475"
     CDS             complement(2640..3836)
                     /gene="dinB"
                     /locus_tag="PLA58_09475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOR63063.1"
                     /translation="MKKVIFLVDMNAFFISCEMTRNPSLVGIPAAVAGDPKRRTGIIL
                     AANYEARAQGVKTAMVNHEALKRCPELTLVSPDHHFYGRKSEAVMHLLSNYTPDIEQN
                     SIDEAWLDMTGSEGLFGKPLNAAQRIMDEIKDRLGLWCSIGIAENKFLAKMAAEMKKP
                     LGITELWEKDIPERLWPLSVKEMYGVGAKTAEKLNRIGIKTIGELAKSDVSVIHQALG
                     KVGDEIYWHANGIDHSIILARTEDDVKSIGREKTLPEDLTDIEKAKMILLELADDVGM
                     TVRKQGKEGRTVHIRLKYSDFRSVTRQMTVAATSVTREIYQAGCSLLEQNWSAGRPVR
                     LIGISVSGFHEHTFSEQLSLFDQRQVNIRSDKNKQIDKAMDKIRDKYGSEKITLATLA
                     EAEKKR"
     gene            complement(3872..4225)
                     /locus_tag="PLA58_09480"
     CDS             complement(3872..4225)
                     /locus_tag="PLA58_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOR63064.1"
                     /translation="MNHRISGDNMNPVENIFADMPDSRKDELIEAILEAPGLRIERIV
                     SHGQSSPDGFWYDQEDNEWVIVLRGNAGLRFEGREDVLALKPGDHIRIDRRRRHRVEW
                     TDPDQETVWLAVHYK"
     gene            complement(4267..4728)
                     /locus_tag="PLA58_09485"
     CDS             complement(4267..4728)
                     /locus_tag="PLA58_09485"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HOR63065.1"
                     /translation="MDQSDKGSPGAERAAWLYLLLDEAEKRGMPTEEFAKAAILRCGC
                     FQGDRLCAGAGTKSLKGLKKILFTLPTRMVFEVKILDCTDDRLDIDFHYCPLVAAWQS
                     QGAGDEQIARLCDIAMQGDRGIAKSFGCTLEPGETIANGHDKCEIRFKRLS"
     gene            complement(4941..5372)
                     /locus_tag="PLA58_09490"
     CDS             complement(4941..5372)
                     /locus_tag="PLA58_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63066.1"
                     /translation="MTNKARTNILSASQDLIAEVKRRFGNATSEIFGIEPESLTNPEA
                     NYILSFGSADAIRNRAAQERNERSRRLLSEGVRGEKTEVSVEQPTREKSNKPSEPMLS
                     VAEDVWEKGVSAAAQKGSRAIDLLTDRLSPELRDTLRFLKC"
BASE COUNT         1210 a         1573 c         1338 g         1404 t
ORIGIN      
        1 gaaatgacaa agaagaagtc gaaatgacaa aaatggccgt tattcaaagc tctctcttca
       61 gcttctttcc ggcgattccg gttgacaagc tgccgggcct ttcttatact gcccgcgaaa
      121 agcaaaagac caacaagggg atggcgcatg gaaaaaacca ttacaaaagc ttacgtggat
      181 gacaaaggat ttgttctgct ggtctgttca aactgcaaca cgattcaaca gcaggaagca
      241 aaatcctatg gaggcgccaa aggcccgatt tcaattacgt gcggcaattg cggcaatgtc
      301 tatgacgttg agatcgaatt tcggaaattc taccgcaaag ggatccgcgt ggacggcttg
      361 tacttcggcg tcagcaaccc caaaatctgg gggaaaatgg ttctgaaaaa cgtctcgatg
      421 cagggatgcg gctttgaaac gatgggcgcc aacccgtttc agccggggga tctgatcaaa
      481 atcgaatttc aactcaatga tgcaaagcgt tccgtcatta aaaaacaggc cgccgtccgc
      541 tccactttca agaaatatgt cggctgccag ttccaggatc ttcccaacgc catggatgca
      601 gacctgggat tttatctgag gactccgtaa ttcctgatgc tttcactaac gatccatttg
      661 cgcacgatcc gggcgggaga gccatgaacg atgtgctcgc ggccatcggc cttgcgccgg
      721 tcctcatcgc tcaggcgctc tatgtccggc gcgtgactcc caggcttccc gagccttccg
      781 gggaacgatc cggcgttcac ggctccggcc cgctgctcaa actgctcatt gtcggcgatt
      841 ccgcggcggc gggcgtcggc gttcctcttc agaaccaggc gctttccgga caactggctc
      901 gcgcgctggg cgaggacttc cgcgtatcct ggaaattgat tgcccaaacc ggtcatcggg
      961 caaaagatgt gctggccgtt ctggaagaaa cggacccgga gaagtttgat gtggtggtca
     1021 cctccatcgg cgtcaacgat gtcacccacg gtaccggcag gcaaacctgg attgcaaggc
     1081 aggaaaaaat catccgtctg cttctttcca aatttcattc tccgtttatc gttttgtccg
     1141 gcattccccc catgcacctc ttccccgccc ttccccagcc cctgcgctgg tatctgggat
     1201 ccagagcgag ccaattcaac agagcattgc agaaagccgt tgaaagttgc agccgttgcc
     1261 gttttgtgcg gattgatttt cctctggagg cgtcctacat ggccgatgac ggctttcacc
     1321 ccggcgcccg cgcctacgcc ttctgggccg accatctggc cgatgtcatc cggcggcaca
     1381 aaaatcaggc ctgattccga aatcttacga gtccattact ctttcaaggg agcgcttccg
     1441 gacagaaaca gtttccacag ggctttgaac gtcggatagt catgcccgcc cgggatggca
     1501 tagacgcggt ccggcggcaa tacctccgcc agcaactgag atccctgccg gtagaaatca
     1561 tccgtgccgt agcccagata aatcattttg tccggatggt cgccgacggc cgtcttgatc
     1621 cagtgccaca acattctctc ccaatcgtct tcatcctgat agacgcccgg attccaaagg
     1681 cgcacgccgc ccgcggcctc gatttcattc aggatgaatt ttttgccgag aaaaggcgcc
     1741 atcagataga tccccgcaac gtcctgcgga tgctccttca gatacaggat cgcgcccagg
     1801 cctcccatcg aaacaccgat gagccagatt ttccggcagc ctcgcatctt cgccggttcg
     1861 atgacatctt ctttcaacct cgcgatcata ctgcggtcga tgtagtagcc gagatgagcg
     1921 tcgggagcgg ccatgtcata agccagtccc cttgcccgga cttcatccac aaatccctct
     1981 tcttcaaaac tttcagcgcc gcttcccatg ccgcgcagaa agaccaccag accctgtcgg
     2041 cagccgccgg gctgatcgta ggtcagcacc tgcagcggct tttcggccca ggtggcgcag
     2101 cccgctaaaa aaaacacaag caaaaataag gccttgtaat atttcatgtt ttcctgcttt
     2161 tccgaaccgg ctcaacgggc cagacataag gcagatcgtc cggttcgagc catccgaacg
     2221 cgccatagta ttccctgtcc ttccgaagca gattggatcg gtgcgaagca tgaaaagatt
     2281 cgtcgcccag ccagagagga tacgtgattt tcccgcggat gacctgtttc ggcattcggt
     2341 tgagatagcc ccgccggacc cactcgtcca tcgccagatt atgatattgc ttcagcgcgt
     2401 tcgtatatcc gcgccacatg gccgcggcgg gatggcggct ccagcccgaa ccatccgcgg
     2461 aaatcgcccg gagaagctgc atcgcctcca ggcgctgttt gcccaagcgc cttgaatcga
     2521 gaacccgcag ggattgggca aagtcgggat agggcaaaaa cgtctgcatg acaaatttct
     2581 ccactacctg gaaaacgtgt tcacaagctt tctccaattc catcggtaaa cggctgaatc
     2641 taacgcttct tttctgcttc agccaatgtg gcgagggtta ttttttcgga gccatacttg
     2701 tctcttatct tgtccatggc cttgtcgatt tgcttatttt tgtcggatcg aatatttacc
     2761 tgccgctgat caaagagcga caactgttca gaaaatgtgt gctcatgaaa accggagacg
     2821 ctgattccaa ttaatcgcac cggacggccc gcactccaat tttgctcgag cagagagcaa
     2881 ccggcctggt atatttccct ggtgacggat gttgcagcaa cggtcatttg ccttgtgaca
     2941 gaacggaagt cggaatattt aagtctgata tgcacggtcc ggccttcttt cccctgtttt
     3001 ctcacagtca tgccgacatc atcggcaagt tccagcagaa tcattttcgc tttttcaata
     3061 tccgtcagat cttccggcaa tgttttttca cgcccgatgg acttaacatc atcctctgtg
     3121 cgcgcaagaa ttattgaatg gtcaattcca ttggcatgcc aataaatctc atccccgact
     3181 tttcccaaag cctgatggat gacactgaca tcggattttg ccagctcgcc tattgtcttt
     3241 atgcctatac ggttcaattt ctcggcggtt ttggcgccta cgccatacat ttcttttacg
     3301 gaaagcggcc agagcctttc cggaatgtcc ttttcccaca gttcggtgat gccaagaggc
     3361 ttcttcattt cagcggccat ttttgccaga aatttgtttt ccgcaatgcc tatcgagcac
     3421 caaagcccaa gacggtcttt gatttcatcc atgatgcgct gtgcggcatt cagtggtttg
     3481 ccaaacagtc cttcactccc tgtcatgtca agccaggcct catcgatgct gttttgttcg
     3541 atgtcaggag tataattcga cagcaaatgc atcactgctt ctgatttccg cccatagaaa
     3601 tgatgatccg gggaaaccaa agtcaattca gggcaacgtt tcaaagcctc gtgattcacc
     3661 atagccgttt tgacgccctg tgcccttgct tcataatttg cagcaagaat gattccggtg
     3721 cgcctcttcg gatcgccggc gacagcggcc gggattccga caagagaagg gttccttgtc
     3781 atttcacagc tgataaaaaa agcattcata tcgacaagaa atataacttt cttcatggct
     3841 gtccctttta tcgagaacgc tttgctcttt attatttata atggacggca agccataccg
     3901 tctcctgatc cgggtctgtc cattccaccc gatgtctccg gcgccggtca atgcggatgt
     3961 gatcgccggg tttcagcgcc aggacgtctt cccgcccctc gaagcgcaga ccggcgtttc
     4021 ccctgagcac aatcacccat tcgttgtcct cctgatcata ccagaatcca tcaggcgaag
     4081 attggccgtg ggagacgatc ctttcaatgc ggaggccggg cgcctcgaga atcgcctcta
     4141 tcagctcgtc tttccggctg tccggcatat cggcaaagat gttttcgacc gggttcatgt
     4201 tgtccccgct tattctgtgg ttcatgcggc ctccctgatc aggttcacgt catcttagct
     4261 atcggattac gataatcttt taaatctgat ttcgcacttg tcgtggccgt tggcgatcgt
     4321 ttcccccggt tccagcgtgc agccgaacga ttttgcgatc cccctgtccc cctgcatcgc
     4381 aatgtcgcac agccgggcaa tctgctcatc gccggccccc tgcgactgcc aggccgccac
     4441 aagcgggcaa taatgaaaat cgatgtccag ccggtcatcg gtgcagtcca ggatcttcac
     4501 ttcaaacacc attctggtcg gcagcgtaaa aaggattttc ttcagccctt tgaggctttt
     4561 ggtgcccgcg cctgcacaca gtcggtcccc ctggaaacac ccgcatctca gaatggccgc
     4621 ttttgcgaat tcttcggttg gcatgcccct cttctctgcc tcatccagaa ggagataaag
     4681 ccaggcagct ctctctgctc caggagaccc cttatcgctt tgatcaatgg attgttgcgc
     4741 ttcgggatat ttttaattct ggtcatggca ccatcctttc acagcagcaa caaaaaattc
     4801 cgttcgattt ggttgagaga ctaacatatt ttacgaattt tcaatatgac tggactgacg
     4861 atcggaattc aatttctttt cccctaaaaa tcttccccgc tcctggcggc ggggattaag
     4921 gggcggggca atctttttta tcagcactta agaaaccgca gcgtatcgcg cagttcggga
     4981 gaaagccggt ccgtcagcaa atcgatggcc cgcgatcctt tctgcgccgc agcagatacg
     5041 cctttttccc agacgtcctc ggccaccgac aacatcggct cggagggttt attggatttt
     5101 tcacgggtgg gttgttctac agaaacttcg gttttttcgc cgcgaacgcc ttctgaaagt
     5161 agtcgtctgc ttctctcatt tctttcctga gctgctcgat ttcggatggc gtcagcagat
     5221 ccgaaggata ggatgtagtt tgcttcgggg ttggtaaggg attccggttc gattccaaag
     5281 atttcgctgg ttgcatttcc aaatctcctt ttcacctcgg caattaagtc ttgactggcc
     5341 gacagtatat tggttcgtgc tttgtttgtc aaactaataa ctctctttta tctacctctg
     5401 tagttacatt ttatttgagg caattcgtgc cgacgactgg tggaaagacg cactgctgct
     5461 gtccatgctt caatggtgcc acgcctttta aggcatgtaa atgacgcgcc cacgcggggc
     5521 gcgac
//
LOCUS       DAOSRM010000118         5373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_13921_length_5373_cov_99.134825, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000118 DAOSRM010000000
VERSION     DAOSRM010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13921_length_5373_cov_99.134825"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /gene="glgP"
                     /locus_tag="PLA58_09495"
     CDS             <1..1608
                     /gene="glgP"
                     /locus_tag="PLA58_09495"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HOR63067.1"
                     /translation="RLRQYYFLASATMQCIVREHKKRFGDVRTLPEKVVLQINDLHPA
                     LAIPELIRILIDEEGLGWDDAYAMAGRVFNYTNHTVMQEALAAWPEHVLRSMLPRVYA
                     IVAEISERFNGRLWQTFSQDRDKIDQLTIMAYDEVRMANLCVAVCHRVNGVSQLHGRI
                     LKTRTFRDFYVMFPAKFTAITNGITQRRWLAAANPDLCKLIADHIGDKFLRDYNELER
                     LKPLADKPRFLADFAEVKRRNKLRLAEQVAKQQGIVLDPDSVFDVQAKRLHEYKRQLL
                     KVLHILFLYNGFTGDAGFALPRPVTFLFAAKASPAYWRAKSIIRLINAVAALVESHPR
                     TRDAIRVAFLENYNVSLAERLIPAADISEQISTAGYEASGTGNMKFMLNGAVTLGTMD
                     GANVEIFERVGQDNIFIFGAGAAEIDRMETENSYRPKDLFDRNEELRGAVSRLVDGTL
                     PGAAADQFQDLYHSLLFGDYDRADRYFLLYDFDAYRAAFENVLAAYTDEPAWTKKGVL
                     NTAGAGFFSADRTIEEYNRQIWGLRPV"
     gene            1631..3631
                     /locus_tag="PLA58_09500"
     CDS             1631..3631
                     /locus_tag="PLA58_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOR63068.1"
                     /translation="MLINYKKEMMHDSTMPEYRDPAGAVVAGAPVVLRFRIKLENVES
                     VYLCLIGDGFHEDHLMRREGDAWQTEITAPSIPDVYWYYFMVRVGGRLSYYGAAAGRT
                     CGIGAVYSAPPPAYQLTVYDKGFEVPRWFQKSVMYQIFPDRFRPGRDSTARQGIAYHL
                     AKGRRAYLHDSWDEEPLFTPLPGETAYNPCDYFGGTLKGIEASLDYLKSLGVNVLYLN
                     PIFEAASNHRYNTADYFRVDPVLGTEEDFRSLCRAAEGRGMHILLDGVFSHTGSDSIY
                     FNREKAYDTPGAANSPDSPYYTWYTFERYPDQYRCWWGFTSLPEINKQDPGWQRYVIT
                     GADSVVKHWLRAGAAGYRIDVADELPDDVLKKIYEAAKQTKSEAVMIGEVWEDATTKH
                     SYGVKRRYALGGILDSVTNYPLRSAMIAFLKGQSDAFDLKHFLVDQAANYPAPLYYAL
                     TNLLSSHDVERVRTALSARIDPHQLTREQQASFVVSDSQNKKGARRQRLAAVLQFCLP
                     GVPCVYYGDEKGMTGFLDPFNRAPFTEKAPYDLTDEYRRLAGLRASADTLQTGHVVYF
                     APDADCLGVLRYIIGGKDALGRPAGDGVYFMAVNRSDAHRLVVFDFAGKHSLFAEADQ
                     QSLLPLFNGTFTCLLTQKSFTFIDALLEMSLDPLGAVWLKVA"
     gene            3681..>5373
                     /locus_tag="PLA58_09505"
     CDS             3681..>5373
                     /locus_tag="PLA58_09505"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOR63069.1"
                     /translation="MTYQETYLRWRACVTEPDLLAELESIRDDDAQIQDRFLRDLPFG
                     TAGLRGVIGAGIARMNRYVVGRATQGLADYLTGVAGAGASAVIAYDSRRFSFEFARET
                     ACVLAANGIRVYLFKQLTSVPELSFAVRHLKASGGVVITASHNPGQYNGYKVYAAYGG
                     QLGPQESLAVMARIQKLDPFLDVKRIGYEEGTAKGQILEIGEEIDRMYYDRMIRLCGA
                     ESAGDLKVVYTPLHGSGLRTVENVFPLAGIRNWYVVPEQRMPDGSFPTVASPNPEDPK
                     AMEMAIALAGKMEADLAIGTDPDADRMGAAVRRPDGTYTMLTGNQIGCLLIHYLLEKR
                     RQSGELRASDYIVKSFVSTRMADAIAAHYGIHCHTVLTGFRFISELIVQKEPTGAEFI
                     FGFEESFGFLAGTFALDKDGALAALLLCKAAQFFRASGQSLTDALEALYREHGYYLES
                     VKNIAFEGLDGMEKMKGIMERLRARPIDAIGGLPVRFTEDYRSRTRTGQDGIVSALEL
                     PSTDAIRLLLDSGAWLCIRPSGTEPKIKIYYAVSASSREAAEGLLKRISTGFEQMI"
BASE COUNT         1083 a         1548 c         1640 g         1102 t
ORIGIN      
        1 cgcctgcggc agtattattt tctggcttcc gccacgatgc agtgcatcgt ccgcgaacac
       61 aagaagcgat tcggcgacgt gcgcacgctg cccgaaaaag tcgtcctgca gatcaacgac
      121 ctgcatccgg cgctggcgat tccggaactg atccgcatcc tcatcgacga agaaggcctg
      181 ggatgggacg atgcctacgc catggccggc cgcgtgttca actacaccaa tcacaccgtc
      241 atgcaggagg ctctggcggc ctggcctgaa catgtgctcc ggtcgatgct gccgcgcgtt
      301 tacgcaatcg tcgccgaaat ttccgaacga ttcaacgggc gcctctggca gacgttttct
      361 caggatcgcg ataaaatcga tcagttgacc attatggcct acgatgaagt ccgcatggcc
      421 aatctctgcg tggcggtatg ccatagggtc aacggcgttt cgcagcttca cggccggatt
      481 ctgaagacgc ggaccttccg ggatttttat gtcatgttcc cggcgaaatt cacggccatc
      541 accaacggca tcacccagcg gcgctggctg gccgcggcga atcccgacct gtgtaagctg
      601 atcgccgacc atatcggcga taaatttctc cgggactaca acgaactgga gcggctcaag
      661 ccgctggcgg ataaaccgcg gtttttggct gatttcgcgg aggtcaagcg ccgcaacaaa
      721 ctgcgtctgg ccgaacaggt ggccaaacag cagggcattg tcctggatcc cgactcggtt
      781 tttgacgtgc aggctaaaag gcttcacgaa tacaaacgcc agcttttgaa agtgctgcac
      841 attctgtttt tgtacaatgg ttttaccggc gatgcgggct ttgctctgcc ccggccggtg
      901 acgtttctgt ttgccgccaa ggcctcgccg gcctactggc gggccaagag catcatccgg
      961 ctcatcaatg ccgtggccgc cctcgtggag tcgcatcccc ggacccggga tgctatccgc
     1021 gtcgctttcc tggaaaatta caacgtctcc ctggccgaac gcctgattcc cgcggcggac
     1081 atcagcgaac agatctccac ggcggggtat gaagcttccg gaacgggcaa catgaagttc
     1141 atgctcaacg gcgcggtgac gctcggaacc atggacggcg cgaatgtgga gatcttcgaa
     1201 agggtggggc aggacaacat ttttatcttt ggcgcgggcg ccgcggagat cgaccggatg
     1261 gaaaccgaaa acagctatcg tccgaaagat ctctttgacc ggaacgagga actccggggg
     1321 gccgtttccc ggctggtgga cggaacgctg cccggcgccg cagccgacca gtttcaggac
     1381 ctttatcatt ctctgctgtt tggcgattac gatcgggccg accgttactt tctgctctat
     1441 gattttgacg cctaccgcgc cgcgtttgaa aatgtgcttg ccgcctacac ggatgaaccg
     1501 gcctggacaa agaagggcgt gctcaacacg gccggcgcgg gattcttcag cgcggaccgg
     1561 accatcgaag agtacaatcg tcagatctgg ggtctccggc ccgtctgatt tgcaagacag
     1621 gacgccagcg atgctgatca actacaaaaa agaaatgatg cacgactcga cgatgccgga
     1681 atatcgcgat ccggcagggg ccgtcgttgc cggcgcgccg gtggtcctgc gcttccggat
     1741 aaaactggaa aacgtggaaa gcgtctatct gtgtctgatc ggggacggtt ttcacgagga
     1801 tcatctgatg cggcgcgagg gcgatgcctg gcagacggaa atcaccgcgc cttcgattcc
     1861 cgatgtgtac tggtattatt tcatggtccg cgtgggagga agactcagtt attacggcgc
     1921 ggcggcagga agaacctgcg gaatcggcgc cgtttacagc gcgccgccgc ccgcatacca
     1981 gctaaccgtt tacgataagg ggtttgaggt tccccggtgg tttcagaaaa gcgtaatgta
     2041 tcagattttc cccgaccgtt tccggcccgg ccgcgattcg acggcccggc aggggatcgc
     2101 gtatcacctt gccaaaggcc gccgcgccta tctgcacgac tcatgggacg aagagccgct
     2161 cttcaccccg ctgccggggg aaacggccta caatccctgt gactatttcg ggggcaccct
     2221 caaaggcatc gaggcgtctc tggattatct gaaaagcctg ggcgtgaacg tattgtattt
     2281 gaatccgatt ttcgaggcgg cctccaacca ccggtacaat accgccgact acttccgcgt
     2341 cgatccggtg ctgggcacgg aggaggattt tcggtccctg tgccgtgcgg ccgagggaag
     2401 ggggatgcac attcttctgg acggcgtgtt ttcccatacc ggctccgaca gcatttattt
     2461 taaccgggaa aaagcctatg acacgccggg cgccgccaac agtccggatt ctccctacta
     2521 cacctggtac acgtttgagc ggtatcccga tcagtaccgc tgctggtggg ggtttacgtc
     2581 attgcccgaa atcaacaagc aggatccggg ctggcagcga tatgtcatca ccggcgccga
     2641 cagcgtcgtg aagcactggc ttcgggcggg tgcggcaggc taccgcatcg atgtggccga
     2701 cgagctgccc gacgacgtgc tgaaaaaaat ctacgaagcc gcgaaacaga cgaaatcgga
     2761 ggcggtgatg atcggcgagg tctgggaaga cgcgaccaca aagcacagct acggcgtaaa
     2821 aagacgctac gcgctcggag ggatcctgga ttcggtcacg aattatccgc tgcgcagcgc
     2881 catgatcgct tttctgaaag gccagtccga cgcctttgac ctgaagcatt ttctggtgga
     2941 tcaggcggcc aattatcccg cgcccctgta ctacgccctg accaatcttc tttcctccca
     3001 cgatgtggag cgcgtgcgca ccgcgctcag cgcgcgcatc gatccgcatc agttgacccg
     3061 agagcagcag gcttcttttg ttgtcagcga cagccagaat aaaaaaggcg ccaggcgcca
     3121 gcgcctggcc gcggtgctgc agttctgcct ccccggtgtt ccctgcgtct attacggcga
     3181 tgagaaaggc atgaccggtt ttctcgatcc cttcaaccgt gcgcctttca ctgaaaaagc
     3241 gccgtacgac ctcacggacg aataccgaag gctggccggg ctgcgcgcct cggcggacac
     3301 gctgcaaacg ggccatgtcg tgtatttcgc ccctgatgcc gactgtctcg gcgttttgcg
     3361 ctacattatc gggggaaagg acgctctggg tcggccggcc ggggacggcg tctatttcat
     3421 ggcggtcaac cgttccgatg cgcaccggct ggtggtcttc gattttgcgg gaaagcattc
     3481 gctctttgcc gaagcggatc agcaaagcct gctcccgctg ttcaacggga cgttcacctg
     3541 cctcttgacc caaaagtcct ttacgttcat cgacgcgctt ctggaaatgt cactggatcc
     3601 cctcggggcc gtgtggctga aggtcgcctg aagagcgcgg ggagcccgga aggggacaat
     3661 cccagaaaag gaaatgcaaa atgacgtacc aggaaactta tttgcgctgg agagcatgcg
     3721 ttacggaacc ggatctgctc gccgaactgg agtctattcg ggacgatgac gcgcagattc
     3781 aggaccgttt cctgagggac ctgccctttg ggacagcggg actgcgcggt gtgatcggtg
     3841 cgggtattgc ccgaatgaac cgctatgtcg tcggccgggc gacgcaggga ctggcggatt
     3901 atctgaccgg tgtcgccggc gccggggcgt ccgcagtgat tgcgtacgat tcccgaagat
     3961 tttcttttga atttgcaaga gaaacggcct gtgtgctcgc ggccaacggc atcagggtgt
     4021 atctgttcaa acagttgacg agcgttccgg agctgtcctt tgccgtgcgc cacctgaaag
     4081 cgagcggcgg cgtcgtgatc acggcaagcc ataatcccgg acagtacaac ggctacaagg
     4141 tttatgccgc ctatggggga cagctgggtc cgcaggagag cctcgccgtg atggcccgca
     4201 tccaaaagct ggaccctttt ttggacgtga aaaggatcgg ctatgaggaa gggaccgcaa
     4261 aaggacagat tctcgagatc ggcgaagaga tcgaccggat gtattacgat cgcatgatcc
     4321 ggttgtgcgg ggccgagagt gcgggcgacc tgaaagtggt ttatacgccg ctgcacggat
     4381 cagggctgcg cacggtggaa aatgtatttc ccctggcggg gatccgcaac tggtatgtcg
     4441 ttcccgagca gcgaatgccg gacggttcgt tcccgacggt tgcgtctccc aatcccgaag
     4501 acccgaaagc gatggagatg gccattgccc tggctggaaa gatggaggcg gacctggcca
     4561 tcggcacgga tccggacgct gaccggatgg gggccgccgt tcgccggccg gacggcactt
     4621 acacgatgct gacgggcaac cagatcggct gcctgctgat tcattatctc ctcgaaaagc
     4681 gccgacagtc tggagaactt cgagcctccg actacatcgt caaatcgttt gtctccaccc
     4741 gcatggcgga cgcgatcgcc gctcattacg gaattcactg ccacaccgtg ctgacgggat
     4801 ttcgctttat ctccgaactc attgtgcaaa aggagcccac gggcgcggag tttattttcg
     4861 ggtttgaaga aagcttcggg tttctggccg gcacctttgc gctggacaag gacggggcgc
     4921 ttgccgcgct cctcttgtgc aaggcggccc agttcttccg ggccagcggc cagagtctga
     4981 ccgacgcgct ggaggccctg taccgggagc acggctatta cctggaaagc gtcaagaata
     5041 ttgcttttga aggtctggac ggcatggaga agatgaaggg catcatggag cggttgaggg
     5101 ccaggcccat tgacgcgatc ggcggcctgc ccgtccggtt cacggaggac taccgttcgc
     5161 gtacccgcac cggtcaggac ggcatcgtgt cggcgctgga gctgccgtcc acggacgcga
     5221 tccggctgtt gctggattca ggcgcgtggc tctgcatccg tccgtccggg acggagccga
     5281 agatcaagat ctactacgcg gtttccgctt cttcccggga agcggcggaa ggcctgttaa
     5341 agcgtatttc cactggtttt gagcagatga tct
//
LOCUS       DAOSRM010000119         5347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_14002_length_5347_cov_101.417989, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000119 DAOSRM010000000
VERSION     DAOSRM010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14002_length_5347_cov_101.417989"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..925)
                     /locus_tag="PLA58_09510"
     CDS             complement(86..925)
                     /locus_tag="PLA58_09510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR63070.1"
                     /translation="MRITVRNMTPKEVSLAIEWAAAEGWNPGLHDAECFYAADPQGFF
                     LAEADGQPAGCLSAVAYDEHFGFAGFYIVLPQFRNLGIGHELVRTASAYAGSRSMGND
                     AVVAQQETYRQLGFRLAYRNIRYRGKASSKAGQAPEIIDLRKIPFGDLVVYDRAMFPA
                     ERPVFLQHWINQPEATALGCEDNGKLAGYGVIRKCRRGYKIGPLFADREEIAETLFLA
                     LTGRIPGEEFFLDIPEPNAAAGSLVRRHNMQSVFETARMYSGAVPSLPLERIFGVTSF
                     ELG"
     gene            1154..2107
                     /locus_tag="PLA58_09515"
     CDS             1154..2107
                     /locus_tag="PLA58_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR63071.1"
                     /translation="MKKETGHTRFGSVLVIPGEDECRFPFCTSLLIDDDVRVLVDPGA
                     GLSTLVSLQKKAPVDLVINTHYHFDHIAYNYLFDRARILLNEQEARCFREKKYLASLL
                     GMEEVYGPEWVDGWIARIADTETRQSPFSPQNRHEWWLSASRVDEEYRWGDVLDFGKT
                     KMQVIGAPGHSKGFSCLNFPDLGVMYVADIDLSSFGPWYAGSDGDIDLFLSSCEAVRA
                     VDCDHFITGHERGVLNRKDFLDGLDRFIAVIDERDRKILSVLQKPLSLTEIAERGLIY
                     GRKYHVDAWIYMWEFIMIRKHVQRMVGQGRILDLGDQLIAA"
     gene            2133..3467
                     /locus_tag="PLA58_09520"
     CDS             2133..3467
                     /locus_tag="PLA58_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOR63072.1"
                     /translation="MLEFARPQETLSVVDAAAIVVGMVLGVGIFKTPSIVAASAESEG
                     MLMLLWLAGGLASVVGALCYAELMSSCPHAGGDYHYLARAFGFPPAFLYAWARLAVIQ
                     TGAIAMVAFLAGDYASEIFRLGPLSSSLYAALFIVFLTAVNIAGIRQGKVTQMLLAAG
                     ILFGLLVVSAAGILADGPRAPVSSGGIVPGRQALGTAMIFVLLTYGGWNEASFLSAEV
                     RQGPHNPLKVLLFSIGTVTAVYLLVNVALLNSLGLSGMVSSKAVMADMMRGLLGASAG
                     AVVSLLILLAAVSTMNASIMTGARTAYALGRDFPVLGILGRWQGRRQTPVNALLVQGG
                     VALALVALGTGAHDGFVMMVEYSAPVFWLFFLMVGVSVFLLRRRYPDLPRPFRVPLYP
                     LTPLVFIAICAFMLYASLAYTGRGAWVGVCVLLSGIPLWVWQKQRRTADKYS"
     gene            3502..4293
                     /locus_tag="PLA58_09525"
     CDS             3502..4293
                     /locus_tag="PLA58_09525"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOR63073.1"
                     /translation="MQRSLDKGGSGTPVDSGFRVSCLPGKPVLVFILVFCCLFLPVWS
                     PAADTTEAEYAAQRENMVARQIEDRGISDSGVLRAMRAVKRHEFVPESLRGGAYADHP
                     LPIGEGQTISQPYIVALMTEAIRPKPHMKVLEIGTGSGYQAAVLAELCKSVYSIEIVE
                     SLGRRAAKLLSGRYRNVHVRIGDGYQGWREEAPFDAILVTCAPTKVPRPLEEQLREGG
                     RMVIPVGESRVQELVVLTKRNGRLERQEIIPVRFVPMVDAKGRKY"
     gene            complement(4312..4587)
                     /locus_tag="PLA58_09530"
     CDS             complement(4312..4587)
                     /locus_tag="PLA58_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63074.1"
                     /translation="MPDYRAKISETTDGDPEIHHYLVTAKDEAEARKYIMKFMERFID
                     DDNDPEIIENGYTFYNKAVVVRLESIKETTKDKFKEFLLKLHTINMR"
     gene            complement(4769..>5347)
                     /locus_tag="PLA58_09535"
     CDS             complement(4769..>5347)
                     /locus_tag="PLA58_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOR63075.1"
                     /translation="QDLKDYAVSGKAHSYRITERAVVDFDKPLVAAAKGVAVGGGATI
                     LFHTDVLYVGESLRMRLPFNSLGMAPEFASSYMLQMRIGPQRAAELLFTTEWIDADKA
                     LATGIASRKFTDDELLPGAMAKAAEIAQLPLSSLMETKRCLKTAHRTGIEAALEVERE
                     AMDRLAGGPASIEAMMAFMEKRKPNFRNLKTK"
BASE COUNT         1005 a         1470 c         1593 g         1279 t
ORIGIN      
        1 gctataaaaa tcttatccgc aatcctgcgc taagtttttt aacacatcag tttatgttta
       61 atattttact cctggccttt gatcactatc ccagttcaaa gctggtcacg ccgaagattc
      121 tttccagcgg cagggaaggg accgcgccgg aatacatgcg ggcggtttcg aaaacactct
      181 gcatgttgtg gcggcgaacc agcgaaccgg cggccgcatt gggttcgggg atatccagga
      241 aaaattcttc gccggggatc cggccggtca gggccaggaa taaggtttcg gcaatctctt
      301 cccggtcggc aaacagcggg ccgattttat agccccggcg gcacttgcga attacgccgt
      361 atccggccag ttttccattg tcctcgcagc ccagcgccgt cgcttccggc tggttgatcc
      421 agtgttgcag aaaaacgggg cgttcggccg gaaacatggc gcggtcgtaa acgaccaggt
      481 cgccgaaagg gattttccgc aggtcgatga tttcaggcgc ttggccagct ttggaggacg
      541 cctttccgcg atagcggata ttgcggtaag ccagacggaa acccagttgt ctgtaggttt
      601 cctgctgagc gacaacggcg tcgttgccca tgctgcggct tccggcatag gcggatgctg
      661 tccggaccag ttcatgcccg attcccaggt tgcggaattg gggcaggaca atataaaacc
      721 ccgcaaagcc gaaatgttcg tcataggcca ccgcggaaag gcatccggcc ggctgtccgt
      781 ccgcttcggc caggaaaaac ccctggggat cggccgcgta aaaacattcc gcgtcatgca
      841 ggccgggatt ccagccctct gcggcggccc attcaattgc cagggaaacc tccttcgggg
      901 tcatattgcg aaccgttatg cgcatggatt tacctccgga tgaactgcgg ctgcggcagc
      961 cttctttgac gttgttttct agcacttccg tcaggcgaca tcaattggat agttgatctt
     1021 tagggtgaat tctgttaccg gattttcaaa cagacccggc ggaagaagcg cgtcgttgag
     1081 cgagctacat tacttcctgc gacttcaggg tggacggcat cctgatgccc cggccatttt
     1141 tgaagatatt tttatgaaga aagaaaccgg acatacgcga tttggctccg tcctcgtcat
     1201 tccgggagaa gacgaatgca gatttccctt ctgcacgtcc ttgttgatcg atgacgatgt
     1261 cagggttctg gttgatccgg gcgcgggcct ttcgacgctt gtttcgctgc aaaagaaagc
     1321 gcccgtcgat ttggtgatca atacccatta tcacttcgac cacattgcgt ataattacct
     1381 ttttgaccgg gcgcggattc tgctcaatga gcaggaagcc cggtgcttcc gggagaaaaa
     1441 atatctggcg tccctgctgg gcatggaaga ggtttacggc ccggaatggg tggacggatg
     1501 gatcgccagg atcgccgaca cggaaacccg acagagtcct ttttctcccc agaacaggca
     1561 cgaatggtgg ctgtccgcgt cccgtgtgga cgaagagtac cggtggggcg atgtgctgga
     1621 tttcggcaaa accaaaatgc aggtcatcgg cgcccccggc cattcaaaag gcttcagctg
     1681 cctgaatttt cccgatctcg gcgtgatgta tgtggccgac atcgaccttt cgtctttcgg
     1741 tccgtggtac gccggctccg acggcgacat cgatctcttt ctctcctcgt gtgaagccgt
     1801 ccgggcggtg gattgcgacc atttcatcac tggtcatgaa agaggggttt taaacagaaa
     1861 agactttctg gacggactgg acagatttat cgccgtcatt gacgagcgcg acaggaagat
     1921 tctctccgtc ctgcaaaagc ctttgtcgct cacggagatt gccgaacggg gactcattta
     1981 cggccgcaaa taccacgtcg atgcctggat ctacatgtgg gaattcatca tgatcagaaa
     2041 gcatgttcag agaatggtcg gacagggccg gattttggat ttaggtgatc aattgatcgc
     2101 ggcctgacct ttccggagaa aggaatgtcc cgatgcttga attcgccaga ccccaagaga
     2161 cgctttccgt ggtggatgcg gcggccattg tcgtgggcat ggtgctgggc gtgggcattt
     2221 tcaagacccc gtccattgtg gccgccagcg ccgaaagcga agggatgctg atgctcttgt
     2281 ggcttgcggg cgggctggct tcggtggtcg gcgccctgtg ctacgcggag ctgatgtcct
     2341 cctgtccgca cgccggggga gattaccact atctcgccag agcgttcggt tttcctcccg
     2401 cttttctcta cgcctgggcg cgcctggccg tcatccagac cggcgctatc gcgatggtgg
     2461 cgtttctcgc cggcgactat gcctcggaaa ttttccgtct gggtcctctg tcctcctcgc
     2521 tgtacgccgc gctgttcatt gtttttctca cggctgtcaa tatcgcgggc atccggcagg
     2581 gcaaggtgac gcagatgctg cttgccgcgg gaatcctctt cggattgctg gtcgtgtcgg
     2641 ccgcgggaat tctggctgac ggaccgcgag cgcctgtttc ctccggcgga atcgttccgg
     2701 gacggcaggc gctgggcacg gccatgatct tcgtgctgct gacttacggc ggctggaacg
     2761 aggcgtcgtt tctttccgcc gaagtgcggc agggcccgca caatccgctg aaggtcctgc
     2821 ttttcagcat tggaacggtt acggccgttt atctgctcgt caacgtcgct cttttaaaca
     2881 gcctgggcct gtccggcatg gtttcctcca aagcggtgat ggcggacatg atgcgaggcc
     2941 tgctgggcgc gtccgccggc gctgtcgtca gcctgctgat tctgctggcg gccgtcagca
     3001 cgatgaatgc gtccatcatg accggcgcga gaaccgctta cgcgctgggt cgggattttc
     3061 cggtgctggg gattctgggg cgatggcagg gaaggaggca aacaccggtc aacgccctcc
     3121 tcgttcaggg gggcgttgcg ttggctcttg tcgcgctggg aacgggcgcg catgacgggt
     3181 tcgtcatgat ggtggaatac tcggcccccg tgttctggct gtttttcctg atggtcggtg
     3241 tttccgtctt cttgctgagg cgcagatatc cggatttgcc ccggcctttt cgcgtgccgc
     3301 tgtatccgtt gacgcccctg gtgtttatcg ccatatgcgc cttcatgctg tacgcaagtc
     3361 tggcctatac cggccgcggc gcctgggtgg gcgtttgcgt gctgctttcg ggaatcccac
     3421 tttgggtttg gcagaagcaa agacggacgg cggacaaata ttcataaaac caaaatgatc
     3481 aaaccattga ggggaagaaa aatgcaaaga agtcttgata aaggcggctc cggaaccccg
     3541 gtggattcgg gcttccgcgt ttcgtgcctg ccgggaaaac cggttttggt ttttatcctg
     3601 gtcttctgct gcctctttct tcccgtatgg agtccggcgg cggatacgac ggaagcagag
     3661 tacgccgctc aaagagagaa catggttgcc cggcaaatcg aggacagggg gatttccgat
     3721 tccggagtcc tgcgggccat gcgcgctgtc aagcggcatg agtttgttcc ggaaagcctg
     3781 cgcggtggag cctacgcaga ccaccccctg ccgattgggg aggggcagac catttctcag
     3841 ccctacattg ttgctttgat gacggaagcg attcggccga agccccacat gaaagtgctg
     3901 gaaatcggga caggttccgg ctaccaggcc gcggttctgg ccgagctctg caagagtgtt
     3961 tattccattg agattgtcga gtcgctgggc agaagagccg caaagctcct ctccggtcgc
     4021 tacaggaatg ttcatgtccg gatcggtgac gggtaccagg gatggcgtga ggaggcgccc
     4081 tttgatgcga tcctcgtcac ctgcgccccg acaaaggttc cccgcccgct ggaagaacag
     4141 ctccgggagg gcggccggat ggtgattccc gtcggagaat cccgggtgca ggaactggtc
     4201 gtgctgacca aaaggaacgg ccgccttgaa cgacaggaga ttattccggt ccgctttgtg
     4261 cccatggtgg acgcaaaggg ccggaaatat tgatccaatc cgcctgccgg ttcaacgcat
     4321 attgatggtg tggagcttca ggagaaattc cttgaatttg tctttcgtcg tctctttgat
     4381 ggattcgagc cggacgacca cggccttgtt gtaaaacgtg tatccgtttt cgatgatttc
     4441 gggatcgttg tcgtcgtcga tgaaacgctc catgaacttc atgatgtatt ttctcgcctc
     4501 tgcttcatct ttggcggtca ccagataatg atgaatctcg ggatctccat ccgtcgtttc
     4561 gctgattttt gccctgtagt caggcatatc atccttctcc ttttttttat gaatgctgcc
     4621 ggccggtata tactccattc atttgccgaa ggcaacggtt ccgttgggca gcctgtaaaa
     4681 atttttccac atcgggaata cccgtgattg caattcggct ccggcgagga acgtggatgc
     4741 ccgccggggc atcgaaaaac gattccggtt atttcgtctt caaattgcgg aaattcggtt
     4801 tgcgcttttc catgaaggcc atcatggctt cgatgctggc cggaccgccg gcgagccggt
     4861 ccatcgcttc ccgctcgact tctaaggccg cctcgatgcc cgtccgatgg gcggttttga
     4921 ggcatctttt tgtttccatg agggaactca gaggcagttg cgcaatctcc gcggcttttg
     4981 ccatcgcgcc gggaagaagc tcgtcatccg tgaacttgcg ggaagcgatt cctgttgcca
     5041 gggccttgtc ggcgtcaatc cattccgtgg taaacagcag ctccgccgcg cgctgaggtc
     5101 caatccgcat ctgcagcatg tagctgctgg cgaactccgg cgccattccc aggctgttga
     5161 agggcaggcg catccgcagg ctttcaccga cgtagaggac atcggtgtgg aacagtatcg
     5221 tcgcgccgcc cccgacggca acccctttgg ccgcggcgac cagcggtttg tcaaagtcca
     5281 cgaccgcgcg ttccgtgatg cggtagctgt gcgccttgcc ggaaacggcg taatccttca
     5341 gatcctg
//
LOCUS       DAOSRM010000120         5331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_14062_length_5331_cov_103.960576, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000120 DAOSRM010000000
VERSION     DAOSRM010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14062_length_5331_cov_103.960576"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..360)
                     /locus_tag="PLA58_09540"
     CDS             complement(145..360)
                     /locus_tag="PLA58_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOR63076.1"
                     /translation="MSMEITAPMPGKIASIVVNVGTQVQEEEEVIIMDAMKMEIPVYA
                     PGAGTIKEIKVKVGDSVNEGQVLAILE"
     gene            complement(654..1883)
                     /locus_tag="PLA58_09545"
     CDS             complement(654..1883)
                     /locus_tag="PLA58_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR63077.1"
                     /translation="MMNMPSTVKISLRALRVNKMRSALTMLGIIIGVAAVITMLAVGR
                     GASNKISEQIASVGSNLIIVLPGSTTASGIRLGSGATQNLTRSDAEAIKQECSAVATT
                     APILNGSAQIIYGNSNWPSIVFGTDENMLEVREWTLASGRNFYDQEIRSAAKICLLGQ
                     TVVDNLFGNDDPLHKTVRIKKVPFVVIGVLAPKGQSMLGQDQDDIVYVPVSSAQRNLF
                     GRWFPGTVRSIMVKARSTEDLDRAEQQIKELLRQRHRIGPDQEDDFTVRNLTQMMQMA
                     EQASRVMALLLAAIAGVSLLVGGIGIMNIMLVSVTERTREIGIRMAVGAKTWDIRLQF
                     IIEAVILSLIGGLIGVLLGVLFAQLLSFFAGWTILISTYSILLSFGFSGLVGIFFGFY
                     PAYKASLMNPIDALRHE"
     gene            complement(1880..2569)
                     /locus_tag="PLA58_09550"
     CDS             complement(1880..2569)
                     /locus_tag="PLA58_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR63078.1"
                     /translation="MALIETDHLEKRYEVGDSIVHALDKVSLQIHRGEFVAVMGHSGS
                     GKSTFMNVIGCLDEPTAGTYRLDGIDVSSLSRDALAEIRNQKIGFVFQGFNLLARTTA
                     LENVEVPLLYNKDGSKNRRERALEALRTLGLEDRRHHHPNQLSGGQQQRVAIARALVN
                     NAPLILADEPTGNLDTKTSIEIMDLFVRLNEEKKITIVLVTHEPDISTYSKRIIHFLD
                     GKLVSDEANRR"
     gene            complement(2575..3741)
                     /locus_tag="PLA58_09555"
     CDS             complement(2575..3741)
                     /locus_tag="PLA58_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOR63079.1"
                     /translation="MKKILVASVILILILIALFIFLSRREPAPQYITQTADRGDIRAT
                     VSATGTVNAVTTVLVGTQVSGTIKALFVDYNSTVRQGQLLAQIDPASFEAQVAQAAAN
                     LSLARANLEKSKVALRDTVSTFERNKILFGKNFISKHDLDTSETAYLSALAQIKASEA
                     QVEQTRAALNLAQTNLRYTRIISPVNGTVISRSVDVGQTVAASFQTPTLFTIAQDLTK
                     MQIVANIDEADIGRLLVGQPVTFTVDAYPDLTFRGNVSEIRNSPITVQNVVTYDVIVK
                     VDNADLKLKPGMTANVSIVIDDKKGILRVPNAALRVKIQDKSSSAPAPKGPGVWVLEN
                     NKPKRIPLSVGISDNRFTEVVSGKISEGAAIIVEVKSNDQKNAATQTQRPGPRF"
     gene            complement(3738..5018)
                     /locus_tag="PLA58_09560"
     CDS             complement(3738..5018)
                     /locus_tag="PLA58_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR63080.1"
                     /translation="MRKIIFFLFLLFFIPAQSLFATDDIKNGDVLSLERCIAIALKNH
                     PALGAAAGAIHQSESKIGKARAGYYPQLSIQSGYSRIGPPSSSLSNSSYDYYSNALNL
                     NQTLFDFGKTSTAVDIQKLGKQSAQADFQDTASSLTYSVQDAYFSLLKAKMSETVAVE
                     TVGQFQQHFEMARTFFETGKSSKIDVTSAEVNLSNARIQLISAQNALRLARVNLNKAM
                     GVITAPEYEIQENFNMQASDITFEAALARAYENRPDIQSIALKKDALEKTVDLNKKDY
                     LPILSGNAAYGYAGQDSSLNQSWNVGVAVTFPFFSGFSTKYAVEEARAGLDIAKANEE
                     TLRQKVFQEVKSAWLNRHEAFERIEAGKITVRQAEESLELAQGRYATGVGNSIEITDA
                     MIKLNNAKMTYIAALSDFNIAEASLEKAMGAKTP"
BASE COUNT         1104 a         1421 c         1544 g         1262 t
ORIGIN      
        1 cttaagattt ctcgtcgctc acgctcctcg aaatgacatt tttcaaagct ctcgaagaat
       61 ccaccttcaa caggaaacaa aaacccgttt tgcgatgatg aaaatcacaa aacgggttga
      121 ttgttcaaat tgtaaaaaat ctacttattc aaggatggcc agcacctggc cttcattgac
      181 ggaatcgccc actttgacct tgatttcctt gatggttccc gctcccggag catatacggg
      241 gatctccatc ttcatggcgt ccatgatgat cacttcctct tcttcctgca cctgcgtgcc
      301 cacgttcacc acgatgctgg cgattttccc cggcatgggg gccgtaattt ccatactcat
      361 aattcttttc cgcctccctt tatcgatatt ttgttgacgt ccaaaaccga cgatctcgta
      421 aaaagtctag aaatcgcggt tttcgtcata ccggcgaaag ccggtatcca ttaattccaa
      481 caagttctgg gccccggcgt ccgccggggt gacggcttga atgacttttt acgcgttcat
      541 caaaaatgaa tttcaaaacc ggcctctgta gcgccgcttt gaaattgtcg aagcttttac
      601 cgtaaatgcg ggttcaggtc aatacaatta ttcggcggcg ggctcatcgc cgctcattca
      661 tgtcgcaggg cgtcgatggg gttcatcagc gaggccttgt aggccggata gaagccgaaa
      721 aagatgccga ccaggccgga gaaaccgaaa gagagaagga tggagtaggt tgaaatcagg
      781 attgtccagc cggcgaagaa agacaaaagc tgcgcgaaca aaacacccag cagaacgccg
      841 atcagcccgc cgatgagcga gagaatcacc gcctcgatga taaattgcag gcgaatgtcc
      901 caggttttgg cgccgacggc catccggatg ccgatttccc tggttcgctc cgtgaccgaa
      961 accagcatga tgttcatgat gccgatgccg cccaccagaa gcgaaacgcc tgcgatagcg
     1021 gcgagcagga gcgccatcac gcgggaagcc tgctccgcca tctgcatcat ttgcgtcaga
     1081 ttgcggacgg tgaagtcgtc ttcctgatcc ggaccgatgc ggtgccgttg ccgcaggagc
     1141 tccttgattt gctgctccgc ccggtccaga tcctccgtgc ttctggcttt gaccataatc
     1201 gagcgcaccg ttcccggaaa ccatcgtccg aagaggttcc gctgcgcgga ggagaccggc
     1261 acatagacga tgtcgtcctg gtcctgcccc agcatcgact ggcctttcgg ggccagaacg
     1321 ccgatcacga caaacgggac ttttttgatg cgaaccgtct tgtggagcgg gtcgtcgttg
     1381 ccgaaaagat tgtccacgac ggtttgtccc agcaggcaga ttttggccgc gctgcggatt
     1441 tcctgatcgt aaaagttccg gccggaagcc agtgtccatt cccggacttc cagcatgttt
     1501 tcatccgttc cgaagacgat ggagggccag ttggaattgc cgtaaataat ctgggcgctg
     1561 ccgttgagga tcggggcggt cgtggcgact gcggagcact cctgtttgat ggcctcggca
     1621 tccgagcggg tcagattctg tgtggcgccg cttcccaaac ggatgccgct ggcggtggtc
     1681 gaacccggaa ggacgatgat caggttgctg cccacggagg cgatctgctc ggatatctta
     1741 ttgctggcgc cccgaccgac ggccagcatg gtgatcacgg cggccacgcc gatgatgatg
     1801 cccagcatgg tcagcgccga acgcatcttg ttgacgcgca gggcgcgcaa tgaaattttc
     1861 acggttgaag gcatgttcat catcggcggt ttgcctcgtc gctgaccagt ttgccgtcca
     1921 gaaaatgaat aatgcgtttg ctgtaggtcg aaatatcggg ttcatgcgtg accagaacaa
     1981 tggtgatttt tttctcttcg ttgaggcgga caaaaaggtc catgatttcg atgctcgttt
     2041 tcgtatccag gttgccggtc ggttcgtcgg ccaggatcag cggcgcgttg ttgaccagcg
     2101 cccgggcaat cgccacccgt tgctgctgtc cgccggaaag ctgattggga tgatggtggc
     2161 gcctgtcttc caggcccagc gtccggagag cctccagcgc ccgctccctg cggtttttgc
     2221 ttccgtcttt gttgtagagc agggggactt caacgttttc cagcgccgtc gtgcgcgcga
     2281 gcaggttgaa tccctgaaag acaaacccga ttttttggtt gcggatttca gcgagcgcgt
     2341 cgcggctgag agaacttacg tcaatcccgt cgaggcgata cgtgccggcg gtcggctcat
     2401 ccaggcagcc gatgacattc ataaaggtgg acttgccgga gcccgagtgc cccatcaccg
     2461 ccacgaattc cccccggtgg atctgaaggg aaactttgtc cagcgcgtgg acgatgctgt
     2521 cgcccacttc gtatcttttt tccagatggt ccgtctcgat aagcgccatg gccgctagaa
     2581 cctcggtccg ggccgctggg tttgcgtcgc cgcgtttttc tggtcgttgc ttttgacttc
     2641 cacaatgatc gcggcgccct ccgaaatctt tccggagacg acttccgtaa agcggttgtc
     2701 gctgatgccg accgaaagcg gaatgcgttt gggcttgttg ttctccagca cccagacgcc
     2761 ggggcctttc ggcgcaggcg cggaggagga cttgtcctgg attttgaccc gcagcgcggc
     2821 gttgggaacc cgcaggattc cttttttgtc gtcgatgacg atcgagacat tggccgtcat
     2881 gcccggcttg agtttcagat ccgcgttgtc caccttgaca atcacgtcat aggtgacgac
     2941 attttgcacg gtgatcggcg agttgcggat ttccgaaaca tttccccgaa acgtcaggtc
     3001 gggataggcg tccacggtaa aggtgacggg ttggcccacg agcaggcggc cgatgtccgc
     3061 ttcgtcgata ttggccacaa tctgcatttt ggtcagatcc tgggcgatgg taaacagcgt
     3121 cggcgtctga aaactggcgg cgacggtctg cccgacgtcc acgctgcgcg agatgaccgt
     3181 tccgttaacc ggcgaaataa tccgcgtgta gcggagattg gtttgcgcga ggttcagcgc
     3241 cgcccgggtc tgctccacct gcgcttcgga ggccttgatc tgagccaggg cggaaaggta
     3301 agcggtttcg gacgtatcca gatcgtgttt ggaaatgaaa tttttcccga aaaggatttt
     3361 gtttcgttca aaggtggaaa cggtgtcgcg cagggcgact ttggattttt ccagattggc
     3421 gcgggccagc gaaagattgg cggcggcctg ggccacctgc gcctcaaacg aagcgggatc
     3481 gatctgggcc aaaagctggc cttgtctgac cgtggaattg taatcgacaa acagcgcctt
     3541 gatcgttccg gacacctgcg tgccgaccag aacggtcgtg acggcattga cggttcccgt
     3601 ggcggaaacc gtcgcgcgga tgtcgccccg gtccgccgtc tgggtgatat actgcggcgc
     3661 cggttcgcgg cggctgagaa agataaacag ggcgatcaga atcaggatca ggatgacgct
     3721 tgcgacaaga attttcttca tggcgttttt gctcccatgg ctttctccag actggcttcg
     3781 gcgatgttga aatcggacaa ggcggcaatg taggtcattt tggcgttgtt gagcttgatc
     3841 atggcgtcgg tgatttcaat cgaattgcct acgcccgtcg catagcggcc ctgggcgagc
     3901 tccaacgatt cttcggcctg gcgcacggtg attttgccag cctcgatccg ctcaaaagcc
     3961 tcgtggcggt tgagccacgc ggacttgact tcctgaaaga ctttctggcg aagcgtttcc
     4021 tcattggctt tggcaatatc cagaccggct cttgcctctt cgacggcgta tttggtggag
     4081 aagcccgaaa aaaaagggaa agtgacggcg acgccgacat tccaggactg attcagggag
     4141 gaatcctgtc ctgcgtagcc gtaggcggca tttccggaaa ggatgggcag atagtccttt
     4201 ttattgagat cgaccgtttt ttccagcgcg tcttttttca gggcgattga ctggatgtcc
     4261 ggccggtttt cataggcccg ggcaagagcg gcttcaaacg taatatccga cgcttgcata
     4321 ttgaaatttt cctgaatttc gtattcgggg gcggtaatca cgcccatggc cttgttgaga
     4381 ttcacccgcg cgagacgcag ggcgttctgg gcggaaatca actggatgcg ggcgttgctg
     4441 agattgactt ccgcgctggt tacgtcaatc ttggagcttt tccccgtttc aaaaaatgtc
     4501 cgggccattt caaagtgctg ctgaaactgg ccgacggtct ccacggcgac ggtttcgctc
     4561 atcttggctt tgagaagcga gaagtaggcg tcctgaacgc tgtacgtgag cgaagaggcg
     4621 gtgtcctgaa aatccgcttg ggccgactgc ttgcccagtt tctgaatatc cacggcggtg
     4681 gaggttttgc cgaagtcaaa cagcgtctga ttgaggttca gcgcgttgga ataatagtcg
     4741 taggagctgt tgctcaggga cgaagacggc gggccgatgc gagaatagcc ggactgtatg
     4801 gaaagctggg gataataacc ggcgcgggcc ttgccgattt tgctctccga ctgatggatg
     4861 gcgccggcgg ccgcgcccag agcgggatga tttttcaggg caatggcaat acaccgctcc
     4921 agcgagagga cgtctccgtt tttaatgtca tccgtcgcga aaagactctg ggccgggatg
     4981 aaaaacagca aaaagaggaa gaaaattatt ttgcgcatag aagttgtcga caaccctttc
     5041 aaatcgtaaa ccgcactttt acggactcca tatcacaaat caaatctcgt tcacacctat
     5101 atcaaatttt gtgccattgt tgtgtaggga gtttcaaacc cttccctacg ggtccatagg
     5161 caatgggcaa tgggccatag gaagacgagt gatgagtgaa aagcagcgct gggctcttgt
     5221 gacctggcgc ctgaaggcac gcacctctgg ctattgaatg gtaaattgcc gggatcggta
     5281 gcggcggttc cctgcatttt cttttactat ccaccatcca ctattcacta g
//
LOCUS       DAOSRM010000121         5310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_14134_length_5310_cov_118.401713, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000121 DAOSRM010000000
VERSION     DAOSRM010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14134_length_5310_cov_118.401713"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..569)
                     /locus_tag="PLA58_09565"
     CDS             complement(378..569)
                     /locus_tag="PLA58_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63081.1"
                     /translation="MAETPITPPENTWKCSNCGNTVVQAAPPQTCPVCRQKCEFLNVT
                     CYQPDCGFTGMDNRLKPAK"
     gene            706..1125
                     /gene="queF"
                     /locus_tag="PLA58_09570"
     CDS             706..1125
                     /gene="queF"
                     /locus_tag="PLA58_09570"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOR63082.1"
                     /translation="MEKLSKKSDLAGLSLLGSGEKAKPVRKLETFPNRSKRSYTVTLS
                     TEEFTCLCPMTGQPDFARITIAYIPDKKIVESKSLKLYFWSFRNEGVFHEHLTNIILD
                     DLVAALSPRWCKVTAQFAVRGGIAISVEAEYPGNKNK"
     gene            1136..4336
                     /gene="carB"
                     /locus_tag="PLA58_09575"
     CDS             1136..4336
                     /gene="carB"
                     /locus_tag="PLA58_09575"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOR63083.1"
                     /translation="MAKREDIKKVLIIGSGPIVIGQACEFDYSGTQACKALSKLGYKI
                     ILVNSNPATIMTDPGMADVTYIEPLNAQTLTEIIENEKPDAILPNLGGQTGLNLTSEL
                     HRRGILDKHGVKVIGVQVDAIERGEDRIAFKEAMNKLGIEMPKSKPATSVEEAEKIAA
                     EMGYPVVIRPAYTMGGTGGGIVYNLEELRLIASRGISASLIGQILIEESVIGWEELEL
                     EVVRDAKNHMITVCFIENVDAMGVHTGDSYCTAPMLTISEELQKRLQKYSYDIVEAIQ
                     VIGGTNVQFAHDPKTDRVVVIEINPRTSRSSALASKATGFPIALISSILASGITLDEI
                     PYWREGTLDKYSPSGDYVVVKFARWDFEKFPGAVDKLGTQMRAVGEVMSIGKNYKEAL
                     QKAIRSLEKKRYGLGFVKDFHARPLDELMELLSEPSSERQFIMYEALRKGASIDALHA
                     KTHIKKWFIEQMKELVALEEKILACKGKKLPDALLVEAKKDGFSDRYLAQILEKKEEE
                     IRQQRLAAGLAESWDAVPVSGVENAAYYYSTYNAPDKVGVSKNRKIMVLGGGPNRIGQ
                     GIEFDYCCVHAAFALRDEGIESIMVNCNPETVSTDYDTSNKLYFEPLTVEDVLSIYEK
                     EKPDGVIVQFGGQTPLNIAADLAKAGVKIIGTSPETIDLAEDRDRFRAMMDKLGIPMP
                     KSGMASNLQEALSVANGIGYPLMVRPSYVLGGRGMEVVHDEQMLKRYVEAAVDVTPER
                     PILIDKFLENAIEAEADAISDGTDAFVPAVMEHIELAGIHSGDSACVIPPISIPARHI
                     DTINDYTRKIAVEFGVVGLMNIQYAIANDVVYILEANPRASRTVPLVSKVCNISMARL
                     ATQIMLGKKLKDLNLKRHAFRHFGVKEAVFPFNMYQEVDPLLGPEMRSTGEVLGLADS
                     FGLAYYKSQEATGQSLPAEGTVLITVEEKDKGGILEVARAFAKIGFRIKASEGTYKFF
                     AANGIAAERILKMHEGRPNIVDAIKNGEIQLVINTPAGRLSKYDDSYIRKAAIKYKIP
                     YITTVAAAVAAVKGIEACRQGHGRAKSLQSYHAEIK"
     gene            complement(4505..4756)
                     /locus_tag="PLA58_09580"
     CDS             complement(4505..4756)
                     /locus_tag="PLA58_09580"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOR63084.1"
                     /translation="METDKKDSGDGSVPREEMVEVFSTYNPGDIAFVKSLLDGEGIHY
                     YFQGENTAMLVAAGAYARLLVRADQADRIRDILREAGLL"
     gene            4787..5086
                     /gene="yhbY"
                     /locus_tag="PLA58_09585"
     CDS             4787..5086
                     /gene="yhbY"
                     /locus_tag="PLA58_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HOR63085.1"
                     /translation="MEPLKGSQRKVLRAQAHHLKPLVMVGAKGVTDGLIGSVDLALAD
                     HELIKVKFVEFKEAKKEIAAEIAQATKSELVGLIGNIAILYRPNPDPAKRKIQVS"
     gene            5237..5310
                     /locus_tag="PLA58_09590"
     tRNA            5237..5310
                     /locus_tag="PLA58_09590"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5269..5271,aa:Gln,seq:ctg)
BASE COUNT         1237 a         1516 c         1514 g         1043 t
ORIGIN      
        1 cttcattgct atatcccctt tcttgaaata ataatttccg gccatgtatc catgacccgg
       61 ctttcaaatt cccgtacgtt ccaggcgccc atggtaaaat tttcttttgt atccccatcc
      121 ggacctgaag gaaaaccgga tcgccgggga tacgggcgat ccggggcggg agagcgtcca
      181 ggcaagcccc gataacaccg aagttcaatg ccaccctttc ccgcaacttc agggtttgaa
      241 acgggcagga ccccttgctt acaacgagcg ttggttgtac ttattcaggc gttgccgttt
      301 tgtcaagcaa tattttagcg ttgtcgggaa gccggacggg gtttttcagg ggtcgttgag
      361 gcgttggccg gcgaagctta ttttgcgggt ttcagccggt tatccatccc ggtgaagccg
      421 caatccggct ggtagcaggt gacattcaga aattcgcatt tctgtcggca gacagggcag
      481 gtttgcggcg gcgcagcctg cacgacggtg ttcccgcagt tgctgcactt ccaggtattt
      541 tcgggcgggg tgatcggtgt ttcggccatg agcgttgcct ccttgaggtt ttttggtggc
      601 tggtttatct ctaccacatc caaaaacgct ttggcaaccg aataaacttg aaaaccgaat
      661 gaatttccgg taaacaggag agccctcgcg gcgcaggaga aatgcatgga aaaactctcg
      721 aaaaaaagcg atctggccgg actttccctt ctgggatcgg gtgaaaaagc gaagcccgtc
      781 cgcaaactgg aaacctttcc caatcgctcc aaacgcagct ataccgtcac tctctccacg
      841 gaagagttta cctgcctgtg ccccatgacc ggccagccgg acttcgccag gatcaccatc
      901 gcatatatcc cggataagaa aatcgtcgaa tcgaaatcgc ttaaactcta cttctggtcc
      961 ttccgcaacg aaggcgtttt tcatgaacat ctcaccaaca tcattctgga cgacctggtg
     1021 gccgcgcttt cgccccgctg gtgcaaggtg acggcccaat tcgccgttcg cggcggcatc
     1081 gccatttccg tggaggcgga gtatcccgga aataaaaata agtaaaggat ccatcatggc
     1141 aaaacgagaa gacattaaaa aagtgctgat tatcggctcg gggcccattg tgatcggcca
     1201 ggcctgcgaa tttgactatt cgggaaccca ggcgtgcaag gcgctctcca agcttggcta
     1261 caaaatcatt ctggtcaatt ccaaccccgc cacgattatg accgaccccg gcatggccga
     1321 cgtgacctat atcgagccgc tcaacgccca gacgctgacg gagatcattg aaaacgaaaa
     1381 gcccgacgcg attctgccca atctgggcgg tcaaaccggc ctcaacctga cctccgagct
     1441 tcaccgccgg ggcattctgg acaaacacgg cgtcaaggtc atcggcgttc aggtggacgc
     1501 gatcgaacgc ggtgaagacc gcatcgcgtt caaggaggcg atgaacaagc tggggattga
     1561 aatgccgaaa agcaagcccg ccacctccgt ggaggaagcc gaaaagatcg ccgccgaaat
     1621 gggctacccg gtggtgatcc gcccggccta caccatgggc ggcacgggcg gcggcatcgt
     1681 ttacaatctg gaagaactgc ggctgatcgc gagccgcggc atttccgcca gcctcatcgg
     1741 ccagatcctg atcgaagaat ccgtcatcgg ctgggaagag ctggagctgg aagtcgtccg
     1801 cgacgcgaag aatcacatga tcaccgtctg cttcatcgaa aacgtcgatg cgatgggcgt
     1861 tcacacgggc gattcctact gcaccgcgcc gatgctgacg atttccgaag agctgcagaa
     1921 gcgccttcag aaatattcct acgacatcgt cgaggccatc caggtcatcg gcggaaccaa
     1981 cgtgcagttc gcgcacgacc cgaagacgga ccgcgtggtc gtcatcgaaa tcaatccgcg
     2041 cacctcgcgc tcgtcggcgc tggcctccaa ggccaccggc tttccgattg cgctcatctc
     2101 ctccattctg gcctccggca tcacgctgga tgaaattccc tactggcgcg aaggcacgct
     2161 ggataaatat tcgccgtcgg gcgactacgt cgtggtgaag ttcgcccgct gggattttga
     2221 aaaattcccc ggcgcggtgg acaagctcgg aacgcagatg cgcgccgtgg gtgaagtcat
     2281 gagcatcgga aagaactata aggaagcgct gcagaaggcc atccgctctc tggaaaagaa
     2341 gcgctacggc ctgggatttg taaaagactt tcacgcccgg ccgctcgatg agctgatgga
     2401 gctgctcagc gagccttcca gcgagcggca gttcatcatg tatgaagccc tgcgtaaagg
     2461 cgcgtcgatt gacgctttgc acgccaagac ccacatcaaa aaatggttca ttgagcagat
     2521 gaaggagctg gtggcgctgg aagaaaaaat cctggcctgc aaaggcaaaa aactgcccga
     2581 cgccctgctg gtggaggcca agaaagacgg attttccgac cgctatctgg cccagattct
     2641 ggagaagaag gaagaagaga tccgccagca gcgcctggcg gcgggactgg ccgagtcctg
     2701 ggacgccgtg ccggtgagcg gcgtcgaaaa cgcggcctat tactactcca cgtacaacgc
     2761 cccggacaaa gtaggcgtca gcaaaaaccg caagatcatg gtgctgggcg gcggccccaa
     2821 ccgcatcggc caggggattg agtttgacta ctgctgcgtg cacgcggctt ttgccctgcg
     2881 cgacgaaggc atcgaatcga tcatggtcaa ctgcaacccc gaaaccgttt ccaccgacta
     2941 cgacacgtcc aacaagctgt acttcgaacc gctcaccgtc gaagacgttt tgagcattta
     3001 tgagaaggaa aaaccggacg gcgtgatcgt ccagttcggc gggcagacgc cgctgaacat
     3061 cgcggcggac ctggcgaaag cgggtgtgaa aatcatcggc acgtcaccgg agacgatcga
     3121 tctggccgaa gaccgggacc gcttccgcgc catgatggac aaactgggca tccccatgcc
     3181 gaaatccggg atggccagca atcttcaaga ggccttgagt gtggcgaacg gcatcggcta
     3241 tccgctgatg gttcgcccct cgtatgttct gggcggccgc ggcatggaag tggttcacga
     3301 cgagcagatg ctcaagcgct atgttgaagc cgccgtggac gtcactcccg agcggcccat
     3361 tctgatcgac aagtttctgg aaaacgcgat cgaggccgaa gcggacgcca tttccgacgg
     3421 cacggacgct tttgttccag ccgtaatgga gcatatcgaa ctggccggca tccactcggg
     3481 cgattccgcc tgcgtgatcc cgcccatcag catcccggcg cgccacatcg acacgatcaa
     3541 cgactacacc cggaagatcg ccgtggagtt cggcgtggtg ggcctgatga acatccagta
     3601 tgccattgcc aatgatgtgg tgtatattct ggaggccaat ccgcgcgcgt cgcgcaccgt
     3661 accgctggtt tccaaggtct gcaacatctc tatggcgcgg ctcgccacgc agatcatgct
     3721 gggcaaaaaa ctgaaggatt tgaacctgaa gcgccatgcc ttccgccatt tcggcgtcaa
     3781 ggaagcggtt ttcccgttca atatgtatca ggaagtcgat ccgctcctgg ggccggagat
     3841 gcgctccacc ggcgaagtgc tgggactggc cgattccttc ggactggcgt attacaaatc
     3901 ccaggaagcc accggccaga gcctgcccgc ggaaggcacc gtgctcatta ccgtggaaga
     3961 gaaggacaag gggggcattc tggaagtcgc ccgcgcgttt gccaaaatcg gcttccggat
     4021 caaagcctcc gaaggcacgt ataaattttt tgccgcgaac ggcattgccg ccgaacgtat
     4081 tttgaaaatg cacgaaggcc gtccgaatat cgtggatgcc atcaaaaacg gagagatcca
     4141 actggtcatc aacacaccgg ccgggcgcct cagcaaatac gacgactcct acatccgtaa
     4201 ggcggcgatc aagtacaaga ttccctacat caccaccgtt gccgcagcgg tggccgcggt
     4261 taaagggatt gaggcctgcc gccagggcca cggccgggcg aaatccctgc aaagctatca
     4321 cgcggagatt aaataaggct gaagactgaa gattgaaggc tgaaggttga gaagtaggga
     4381 acggtcgcgg ccgttccctg caccctttgt catccccgac ctgatcgggg atccagtagt
     4441 gcgtggcgct cgtgaccttt aggttattag ccgcgcttta acgggtctaa agcccgaact
     4501 gcttctacag aaggcccgct tcccgcagga tgtcgcggat acggtcggcc tgatcggccc
     4561 ggacgagcag gcgcgcgtat gcgcccgcgg cgaccagcat cgcggtgttt tccccctgaa
     4621 aataataatg gatgccttcg ccgtccagca gggatttgac aaacgcgatg tcgccgggat
     4681 tgtaggtgga aaaaacctcg accatttcct ctcggggaac gcttccgtcc ccggaatctt
     4741 ttttatcggt ttccatggcg agcatcatac acgggaaagg atttttatgg aacccctcaa
     4801 aggatcgcag cgaaaagttc tgcgcgcgca ggcgcatcac ttgaagccgc tggtcatggt
     4861 cggcgcgaaa ggcgtgacgg acggcctgat cggctccgtg gatctggcgc tcgcagatca
     4921 cgagctgatc aaggtgaagt tcgtcgagtt taaagaagcc aaaaaagaaa tagcagccga
     4981 aatcgcgcag gcgacaaaaa gcgagctggt cggcctcatc ggcaatatcg ccatccttta
     5041 ccggcccaat cctgatccgg caaagagaaa aattcaagtg tcgtaaggga tttgcgccgg
     5101 ccggatcagg cggatggccg gccgccggca aggcgaagaa aatgggttga cagataaaat
     5161 caatcgcatt atatacagcg cgcctgacgg aagggcagca ccgctccaca ccgtccgggc
     5221 actgttcata tcttgatggg gaatcgttca atggtaggac gccggactct gactccgtta
     5281 atcaaggttc gaatccttgt tccccagcca
//
LOCUS       DAOSRM010000122         5295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_14199_length_5295_cov_109.422519, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000122 DAOSRM010000000
VERSION     DAOSRM010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14199_length_5295_cov_109.422519"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PLA58_09595"
     CDS             complement(<1..1015)
                     /locus_tag="PLA58_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOR63086.1"
                     /translation="MTNHRKEPFWSGVVRSMGIVFGDIGTSPIYTLTVVFALTPRTEA
                     SILGILSLVVWTLLILVTGEYAWLAMSLSYKGQGGEIMLREILRRALKPGRKLAFAGF
                     LAFVGVSLLLGDGVITPAITLMSAVEGILLVPGLENVRWEILILIAVLIAIGLFSLQS
                     RGVDKVAGIFGPVMVVWFSCLAITGLVSITAMPGIFKAVNPYYAFVFLSENGLAGFFV
                     LSEVILCATGGEALYADMGHLGRKPILHAWYFVFGALLLNYMGQGVFAVTHPDVKYLL
                     FGMVRHQAAFLYIPFLLLTFLATIIASQSIISGTFSIVYQGITTRILPLMKVDYTSSR
                     IKSQ"
     gene            1223..2758
                     /locus_tag="PLA58_09600"
     CDS             1223..2758
                     /locus_tag="PLA58_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HOR63087.1"
                     /translation="MTAELCIAKSGEKEILLLPAMANRHGLITGATGTGKTVTLQKMA
                     ESFASIGVPVFLADVKGDLSGIGAAGVASEKLMKRLEGIGVTEFHPRANTAVFWDVFG
                     EKGHPVRATVSDMGPMLLSRLLNLNDTQTGVLTLVFKIADDNGLLLLDLKDLRAMIQF
                     VGDNAKQFTTEYGNISAASVGAIQRGLLTLDEQGGDKFFGEPMLNIADWMQTDTEGRG
                     VVNVLAADKLYNNPKLYSTFLLWMLAELFEQLPEAGDVEKPKLVFFFDEAHLLFNDAP
                     AALLEKIEQVVRLIRSKGVGVYFVTQNPLDIPESVLGQLGNRVQHALRAFTPRDQKAV
                     KTAAETMRPNPAFNTADVIMELGVGEALISFLDEKGRPAQVERSFVIAPGSRLGVLTD
                     AERQAAIQRSTVFGRYEKALDRESAYEKLRASVAEEKAPAKPQAPAGKVPAGKPEPEA
                     QGGGILGGALGDLLFGRTGPRGGKTDGLVQASAKSLARTIGSTVGRQIVRGVLGSLLG
                     GKK"
     gene            complement(2787..3053)
                     /locus_tag="PLA58_09605"
     CDS             complement(2787..3053)
                     /locus_tag="PLA58_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63088.1"
                     /translation="MSKVKPGDVLNCEVCGLSVIVDEECGCAFADLICCEELMVNKGP
                     RLSKKAAAAPKKKTAVKKEKPAAKKPAVKKAPPKPAAKKKPAKK"
     gene            3294..>5295
                     /locus_tag="PLA58_09610"
     CDS             3294..>5295
                     /locus_tag="PLA58_09610"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOR63089.1"
                     /translation="MNQKMKNILQITPLKITLFVILIALALFLMDFQFLRLVELKTLD
                     LRIASRGDLKPGGETVIAVIDEKSLSELGRWPWPRTTIAQMVRKLKQNGAKAVGFDIV
                     FSEPDINANLKTIDALWAEMKKSGISQPGVIDLLRRKRADADTDAILAASIKEAGNVT
                     LGYFFHFARKGSDQDLAHLTAKRIAENARCIENSRYPMVNSSSGKPSDAHMPHAFAPE
                     ANIPVLSAAGRNSGYFNALPDSDGSNRWSPLVIAFENNYYSSLAVSLVWAYLDFPNLS
                     LNLEPFGVNSVTIDDIVIPTNESGQLLINYMGPPQTFPHYSIADILADRLPKDAFRNK
                     IVLVGATAIGIYDLRVTPFSSTFPGVEIHANVIDNILHRNFLIHSSVTRFIDVCSIIL
                     FGLILGILIPRLRPITGMIAAFLMIAAFVVINFFVFFSFNTWLNLVYPLITMATIYLG
                     ITIYHYFKEEREKKKIRGAFQYYLTSSVINEMLKDPGKLKLGGDKKDLTVLFSDIRGF
                     TTISEKMTPEELVALLNEYLTTMTNQVFHYDGLLDKYMGDAIMAVFGAPLDQPDHAER
                     ACRTALAMMKELRKLQDKWKAEGRPVFDIGVGLNSGDMVVGNMGSEMRFDYTVMGDMV
                     NLGSRLEGTNKEYGTNIIISEFTYEKVKDAMCCRELDGVRVKG"
BASE COUNT         1159 a         1486 c         1522 g         1128 t
ORIGIN      
        1 tctgcgattt gatccggctc gacgtgtagt ccaccttcat cagcggcaga atccgtgtgg
       61 tgatgccctg gtaaacgatg gaaaaggttc cgctgataat cgactgggat gcgatgatgg
      121 tggccagaaa ggtcaacagg agaaacggga tatagagaaa cgccgcctga tgccgcacca
      181 tgccgaacag caggtacttg acgtccggat gggtgacggc aaagacgccc tgccccatgt
      241 agttaaggag aagcgcgcca aacacaaagt accaggcgtg caggatgggc tttctcccca
      301 gatggcccat gtcggcatac agggcttcgc cgcccgtggc gcacaggatg acctcggaaa
      361 ggacgaaaaa tccggccagc ccgttttccg aaagaaaaac aaaagcgtag tagggattga
      421 cggccttgaa aatgcccggc atggcggtga tggaaaccag ccccgtgatg gccaggcagg
      481 aaaaccagac gaccatgacc ggaccgaaaa ttccggcgac tttgtccact ccccgggatt
      541 gaagagaaaa aagcccgatc gcgataagaa cggcgatcag tatcagaatt tcccagcgca
      601 cattctccag gccgggaacc agcaggatgc cttccacggc ggacatgaga gtaatggcag
      661 gggtgatgac gccgtctccc agcaagagcg aaaccccgac gaacgccaga aatcctgcaa
      721 aagccagctt gcggccgggt ttgagcgctc tgcgcaggat ttcacgcagc atgatctctc
      781 ctccctgccc cttgtaggag aggctcatgg ccagccaggc gtattcgccc gtgacgagaa
      841 tcagaagggt ccagaccacc agggaaagaa ttcccagaat gctggcctcg gttctcggcg
      901 tcagcgcaaa gacgacggtc agggtataaa tggggcttgt gccgatgtcg ccgaacacaa
      961 tgcccatgga acggaccacg ccgctccaga aaggttcctt cctgtggttt gtcacatacc
     1021 ccccctcaat ttgagcctgc cttaccactg aaaaaccggg cggtcaagcg cggtgaaggg
     1081 ccccggcgcc gggctttttt gctggttttt ccggatggtc ccgctcttcg aatggtcatt
     1141 gacagacggc cggattcttt ctatagttga gcctgcaaga ttgaaccgat tcatccgtaa
     1201 aaatttattt cagggggctg tcatgaccgc ggagctttgc atcgcaaaat ccggagaaaa
     1261 ggaaattctt ctgctgcccg ccatggccaa ccgccacggg ctcattaccg gcgcgaccgg
     1321 caccgggaaa accgtcacgc tgcaaaagat ggccgagtcc ttcgcgtcta tcggcgtgcc
     1381 ggtctttctg gccgacgtca agggagacct ctccggcatc ggggcggcgg gtgttgcttc
     1441 cgagaaattg atgaagcggc tggaggggat cggcgtcacg gaattccacc cgcgcgccaa
     1501 caccgccgtg ttctgggatg tgttcgggga aaaagggcac ccggtgcgcg ccacggtctc
     1561 cgacatgggg ccgatgctgc tttcgaggct gctcaatctc aatgacacac agaccggcgt
     1621 gctgacgctg gttttcaaga tcgccgacga caacggcctg ctgcttttgg atctgaagga
     1681 cctgcgggcc atgattcagt ttgtgggcga caacgccaaa cagttcacca cggaatacgg
     1741 gaacatctcg gcggcgtccg tcggcgcgat ccagcggggg cttttgacgc tggacgagca
     1801 gggcggcgac aagtttttcg gcgaaccgat gctgaacatt gccgattgga tgcagaccga
     1861 cacggagggg cggggcgtgg tcaacgtgct ggccgccgac aagctgtaca acaatcccaa
     1921 actttactcc acttttctcc tgtggatgct ggccgaactt tttgagcagc tgccggaagc
     1981 gggggatgtc gaaaagccaa aactggtgtt cttttttgac gaagcccacc tgctgtttaa
     2041 cgacgcgccg gctgcgcttc tggaaaagat cgagcaggtg gtgcggctga tccgctccaa
     2101 gggggtgggc gtgtatttcg tgacgcaaaa tccgctggac attccggaaa gcgtcctggg
     2161 acagctggga aaccgcgtcc agcacgccct gcgcgccttc acgccgcgcg atcagaaagc
     2221 ggtcaagacg gcggcggaga ccatgcggcc caaccccgct ttcaatacgg cggatgtgat
     2281 tatggagctg ggcgtcgggg aggcgctgat ttctttcctg gatgaaaaag gacgaccggc
     2341 gcaggtggaa agatcttttg tcatcgcgcc cggttcccgc ctcggcgtgc ttaccgacgc
     2401 cgaacggcag gcggccattc agcggtccac ggttttcggg cgctatgaaa aagcgctgga
     2461 ccgggaatcc gcctacgaaa agctgcgcgc ctccgttgcg gaagagaagg ctccggcaaa
     2521 gccgcaggct ccagccggaa aagttccggc cggcaagccc gaaccggagg cgcagggcgg
     2581 cggcattctg ggcggcgcct tgggagactt gctgttcgga cgcaccggac cgcgcggtgg
     2641 aaaaacagac ggcctggttc aggcctcggc caagagtctg gcgcggacga tcggatcaac
     2701 cgtcggacgc cagattgtcc gcggggtcct ggggtctctg ctgggcggaa aaaaataacc
     2761 gcgcgccgca gcatgatccg gtcgggttat ttttttgccg gtttcttttt ggcagccggc
     2821 ttcggagggg ctttttttac ggccggcttt ttggcagccg gtttttcttt cttcaccgcg
     2881 gtttttttct tgggtgcggc ggctgccttt ttcgatagcc gggggccttt attaaccatg
     2941 agttcttcgc agcagatcag atcggcaaaa gcgcacccgc attcctcatc cacaatgacc
     3001 gaaagtccgc atacctcaca atttaaaaca tcaccgggct ttacctttga catttgcagt
     3061 tcctcctcgt tgtttatgag ttttcccttc cgattctgac gtatccgatg ggtttatttg
     3121 tagtgcaaaa ccggttttct ttcaagcgtt gaatgcacgg ggacgctttt ttatgagcgt
     3181 ccgcgcttcc ctggccgacg gatcggggaa cggtaaagcg cgccaaatca aaggacatct
     3241 tgactttaca ggcctctctg gtatgatttt cgcccgaaga aggagaggca ggcatgaatc
     3301 agaaaatgaa gaacattttg cagattacgc cgctgaagat tacgcttttt gtcattttga
     3361 tcgcgctggc gctgtttttg atggactttc agtttctgcg cctggtggaa ctcaaaacgc
     3421 tggatctgcg catcgcgtca cgcggcgacc tgaagccagg cggtgaaacc gtcattgccg
     3481 tcatcgatga aaaaagcctt tccgaactgg gccggtggcc ctggccccgg acaacgatcg
     3541 cgcaaatggt gcgaaagttg aagcagaacg gcgccaaggc ggtcggcttt gacattgttt
     3601 tttccgaacc ggacatcaat gccaatctga aaaccattga cgcgctttgg gccgaaatga
     3661 aaaaaagcgg tatttcccag cccggcgtca ttgatctgct gcgcaggaaa agagccgacg
     3721 cggacacgga cgcgatactt gccgcctcca tcaaggaggc cggcaacgtc accctcggat
     3781 atttctttca ttttgcccga aaaggatccg atcaggatct ggcgcatctc acggcaaagc
     3841 gcatcgcgga aaacgcgcgg tgcatagaaa actcccgcta tccaatggtc aattccagct
     3901 cgggcaagcc cagcgatgcc catatgccgc atgccttcgc gccggaagcc aatattcccg
     3961 ttctgtctgc ggcgggccgc aacagcggct atttcaacgc ccttcccgac agcgacgggt
     4021 cgaaccgctg gtcgccgctg gtgatcgctt ttgaaaacaa ttactattca tccctggccg
     4081 tttccctggt ttgggcctat ctcgattttc ccaatctctc gctgaatctc gaacctttcg
     4141 gcgtaaatag cgtgaccatt gacgatatcg tgattccgac gaatgaatcg gggcagttgc
     4201 tgatcaatta tatggggccg ccgcagacgt ttccccatta ctccattgcc gacattctgg
     4261 ccgaccgtct tccgaaggac gcctttcgca acaagatcgt tctggtcggc gcgacggcca
     4321 tcggcattta tgatctgcgc gtgacgcctt tttcctccac gtttcccggc gtcgaaattc
     4381 acgccaacgt catcgacaat attctgcacc ggaactttct gatccactct tccgtgaccc
     4441 gtttcatcga tgtttgctcg attattcttt tcgggttgat cctcgggatt ttgattcccc
     4501 gtctgcggcc gatcaccggc atgattgccg cttttttgat gatcgccgcc tttgtcgtga
     4561 tcaatttctt cgttttcttc agcttcaaca cctggctgaa ccttgtctat ccgctgatca
     4621 ccatggcgac gatttatctg ggcatcacga tttatcatta cttcaaggaa gagcgggaaa
     4681 agaagaaaat ccgcggcgcg ttccagtact acctgacctc ctcggttatc aatgaaatgc
     4741 tgaaggaccc gggcaagctc aagctgggcg gcgataagaa agacctgacc gtgctgtttt
     4801 cggacatccg cggcttcacc acgatttccg aaaaaatgac gccggaagag ctggtcgccc
     4861 tgctcaatga atacctgacg accatgacga atcaggtgtt ccattacgac ggactgctgg
     4921 acaagtacat gggcgacgcc atcatggcgg tcttcggcgc gccgctggat cagcccgatc
     4981 acgccgagcg cgcctgccgg accgcgctgg ccatgatgaa agagttgcgc aagctgcagg
     5041 ataaatggaa agcggaaggg cgccccgtgt tcgacatcgg cgtcggactc aattccggcg
     5101 acatggtcgt gggcaacatg ggatcggaga tgcgctttga ctacacggtc atgggcgaca
     5161 tggtcaacct gggctcgcgg ctggaaggga ccaacaagga atacggcacc aatatcatca
     5221 tcagcgagtt cacctatgaa aaagtgaaag acgccatgtg ctgccgcgaa ctcgacggcg
     5281 tccgcgtcaa aggca
//
LOCUS       DAOSRM010000123         5284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_14246_length_5284_cov_108.410404, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000123 DAOSRM010000000
VERSION     DAOSRM010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14246_length_5284_cov_108.410404"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..515)
                     /locus_tag="PLA58_09615"
     CDS             complement(303..515)
                     /locus_tag="PLA58_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63090.1"
                     /translation="MHKEYKAIVKQDGDWWIGWIEEVPGVNCQERTHDELISSLRIAL
                     KEALEFNRQDALAAVGTGYQEEKIAI"
     gene            complement(811..2673)
                     /locus_tag="PLA58_09620"
     CDS             complement(811..2673)
                     /locus_tag="PLA58_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOR63091.1"
                     /translation="MGNLLVNTRDQQFLLFEQFGIEKLFESEAYGGFSKDDLLMILSE
                     AEKLAVSVIFPTLKEGDEEGCHFKNGVVTVPKCFREPFKKYSEAGWINPMDSPDVGGQ
                     GIPHTAGYGVMELFCAANYAFCMYPGLTHGAANLINVYGNEEQKKKYMEKCFSGEWTG
                     TMCLTEPGAGSDVGALKTTAKRLPDGKFLITGTKCFISAGDHDLTPNIIHPVLARIEG
                     DPPGTKGISIFIVPKIRVNDDGTLGEPNDVKCGGIEHKMGIKASATATLNFGEDGNCI
                     GELLGEERAGIKIMFQMMNEARLGVGMQGVAIGSAAFQHAVAYAKERIQGTPVWEMKN
                     PAAKAVTIINHPDVRKKLMWMKSHVEGIRALAYFGAYCMDMSKVSKTEAEKANWEGYL
                     ELLTPIIKSYSTDKGLLICSTAMDVYGGYGYCSEYPVEQLMRDEKIACIYEGTNGIQA
                     LDFVGRKLGQRKGANVMNMAGLIQKTIAKYKNDGQLGKAAATLEEASNAVFEVAMFFA
                     AAGKGGDFIAPIYNAGKYLDIFGDMVVGYLLLEGAGIAQEKLNAMYEEKGLVTIGKKI
                     GLQRENQEAAFYAGKIASAKFFINEAVTTVKARCEAIKANDKSAMEMVDLGFTV"
     gene            complement(3023..5203)
                     /locus_tag="PLA58_09625"
     CDS             complement(3023..5203)
                     /locus_tag="PLA58_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOR63092.1"
                     /translation="MTPSNADILIRRVRAEGRRSMTETESKKLLASYGVPVARDRMAT
                     NEREAVLQAQKTGYPVVVKGHGAKLQHKTERGLVKTNLKSAKEVREAFRAIRASAGAD
                     WEGCIVSPLIAGSREFVAGLIRDVQFGPAVMFGLGGIFTEALADTAFRIAPLDRQEAK
                     RMIGEISSRKLLGNFRGEAPVDSERLADVLLGLSRLGLERPDIREVDVNPLIATPKGN
                     IVAVDALVVLDEAPSASGAAAGGKGKQKRQAEIRAALDEMANAKSVAVVGATRPEANG
                     FSGMFGCMRNFGYRGRLYPVNPRLQDIDGIRAYPNLSSLPEIADLVIISVPAPRVADI
                     LRECAATGHKNIHIFSSGFKETGEEEGIRLQKEIETVAAEGGLRVIGPNCMGFYVPKN
                     RMLTWENASTKTGPVSLISQSGGNAQDFSNYLTDVYDIGFAKSISYGNALTLDSTDFL
                     DYLARDRETGIITMYLEGVKDGRLLLEQVKKINRLKPVIIYKSGLTESGARAVSSHTG
                     SLAGGEKIWKAFFRQTGAVRVESLEEMADVAQAFHRLGKVRGKRVAVLGFGGGNGVSV
                     ADDCAKAGLSLPVLSQGLIRKLRKRIPPAGSMIRNPIDAAIAFVHLPLMGEVLDLVAR
                     SGETDSFIVSVPFDWLFKQAKDGAHIETLASYLATEAKKYTRGKPMLVAWRQYEASPK
                     IRRWVPVFKDILMKAGIPVYEGLPKAVRALSKLAEYYEYQGLAK"
BASE COUNT         1083 a         1480 c         1419 g         1302 t
ORIGIN      
        1 ctttcgccgg ggtgacggtg tgggttgctt tttacgaggt cgtcaatgca ttacttttta
       61 agaaaaaacc cggtttctgc cggcatgtct acgttcaacg aatttgacgt attgggatta
      121 cttcaatgat tttacaccaa ggtctttgaa tatttttcgg acaagatgat cattgatttc
      181 attatgccga ggcacagatg agcgcttatt aagagacgga ttccaccacc acgaatgact
      241 gcctccttct ctgagcaatt cacatccttg tgatcgcaaa tgcttcagca ggtcatttcg
      301 tttcatatgg cgattttttc ttcctgatat cctgtcccta ctgctgcaag cgcatcctga
      361 cggttaaatt caagagcctc ttttaaggca atccgaaggc ttgatattaa ttcatcatga
      421 gttcgttcct gacagttaac gccaggcact tcctcaatcc agccaatcca ccaatcccca
      481 tcctgcttta cgatcgcttt atattcctta tgcatggcta acctctttat gtgttttttg
      541 tcattatacc ttatcatcag ataattttgg tattgttttc ttgtggaaaa gaagaatgac
      601 ggtctcgtaa aaagtctgtt ttgcaacttt gttgtcattc ccgcgaaggc gggaatctat
      661 taattcaaaa tagttatgga ctcccgcctc cgcgggagtg acaggaataa tgacttttta
      721 ccagactgtc aagatgagcc cgaaatgctg gaaaaagagg gggtgcggca ttcgcacccc
      781 ctcttgttgg ttcatgatga aacggaaagg ctagacggtg aatccgaggt caaccatttc
      841 cattgcggat ttgtcgttgg ccttgatggc ttcgcagcgc gccttgaccg tggtcacggc
      901 ttcattgatg aagaatttgg ccgaggcgat cttgcccgcg tagaaggcgg cttcctgatt
      961 ttcccgctgg aggccgatct tcttgccgat cgtgacgagg cctttttcct cgtacatggc
     1021 gttcagtttt tcctgagcga tgccggcccc ttccagaagc aggtatccga ccaccatgtc
     1081 gccgaagatg tccaggtatt ttccggcgtt gtaaatcggg gcgatgaaat cgccgccttt
     1141 gccggccgcg gcaaagaaca tggccacttc aaatacggcg ttggaagctt cttccagcgt
     1201 ggcggcggcc ttgcccaact gcccgtcgtt tttgtacttg gcgatggtct tctgaatcag
     1261 gccggccatg ttcatcacgt tggcgccttt gcgctgaccc agcttgcggc cgacaaagtc
     1321 gagcgcctgg atgccgttgg tgccctcgta aatgcaggcg atcttttcat cgcgcatcaa
     1381 ctgttccacc gggtattccg agcagtagcc gtatccgccg taaacatcca tggcggtgga
     1441 gcagatgagc aggcctttat cggtcgagta ggatttgatg atcggggtca aaagctcaag
     1501 atagccttcc cagttggctt tttccgcttc cgttttggag accttgctca tgtccatgca
     1561 atacgcgccg aaataggcga gcgcgcgaat gccttcgaca tgggatttca tccacatgag
     1621 tttcttgcgg acatcggggt ggttgatgat ggtcacggct ttggcggcgg gatttttcat
     1681 ttcccagacc ggcgttccct gaatgcgttc cttggcgtac gccacggcgt gctggaaagc
     1741 ggccgatccg atggcgacac cctgcatgcc gacgcccaga cgcgcttcat tcatcatctg
     1801 gaacatgatc ttgatgccgg cgcgctcttc gcccagcagc tcgccgatgc agtttccgtc
     1861 ttcgccgaaa ttcagggtgg cggttgcgga ggctttgatg cccatcttgt gttcgatgcc
     1921 gccgcatttc acgtcgttgg gttcgccgag cgtgccgtcg tcattgacgc ggattttggg
     1981 aacgatgaaa atggagatgc ctttggtgcc gggaggatcg ccttcgatgc gggccagaac
     2041 ggggtgaatg atgttcggcg tcagatcgtg gtcgcccgcc gaaatgaagc acttggtccc
     2101 ggtgatcagg aatttgccgt cgggaaggcg ctttgccgtt gtcttcagcg cgccgacatc
     2161 cgagcccgcg cccggttcgg tcagacacat cgtgccggtc cattcgccgg agaagcattt
     2221 ttccatgtat tttttcttct gttcctcatt gccgtaaaca ttgatcaggt tggcggcgcc
     2281 gtgggtcaga ccgggataca tacagaaggc atagttggcc gcgcagaaaa gctccatcac
     2341 gccgtagccc gcggtgtggg gaatgccctg cccgccgacg tcgggagaat ccatcggatt
     2401 gatccagccg gcttcgctgt attttttaaa cggttcacga aaacacttgg gcacggtgac
     2461 cacgccgttc ttgaaatggc agccttcttc gtccccttct ttgagcgtcg gaaagataac
     2521 gctgacggcc aacttttcgg cctcgctcaa aatcatcagc agatcatcct tggaaaatcc
     2581 tccgtaagct tccgactcaa agagtttttc aatcccgaac tgctcaaaaa gaagaaactg
     2641 ctggtcgcgt gtgttgacta ataaatttcc cataactgac tcccttctta aaagtggttt
     2701 attttttcaa cgttcattga cgtccgttta cgaagagtgg tttcggaatt tttcacgatc
     2761 actgcggaag acaaatcgat tttgaaacat cccgcctgag gttttcggac cattcgtccc
     2821 gtttcaggat catgactata agtacaaacg tgaccgaaag tcaatcaaaa taaactaccc
     2881 cgcagcagcg gggtattgta gaaggaaacc ctttaataat ttcgccccaa ggagcgggga
     2941 atttacccgt agagattaaa aaaagaggcg ggcggaggcg gtttttcacc gcaggggttc
     3001 accggtccgc agccgggcga gcttattttg caaggccttg atattcatag tattctgcca
     3061 gtttggacaa agcccggacc gccttgggga gaccttcgta aaccgggatt ccggctttca
     3121 tgagaatgtc tttgaaaacc gggacccatc gcctgatttt cggcgaagcc tcgtactggc
     3181 gccaggccac cagcatgggt ttgccccggg tgtatttctt cgcttccgtc gccagataag
     3241 aggcaagcgt ttcgatgtgg gcgccgtcct tcgcctgctt gaacagccaa tcgaacggaa
     3301 cggaaacgat gaagctgtcc gtttcgccgc ttcgcgcaac cagatccagc acctcgccca
     3361 tcaggggcag atgaacaaaa gcgatcgccg cgtctatggg attgcggatc atcgaaccgg
     3421 ccggcggaat gcgcttgcga agtttccgga tcaggccttg cgacaaaacg ggaagcgaaa
     3481 ggcccgcttt cgcgcagtca tccgccacgg acacgccgtt gcctccgccg aatccgagaa
     3541 cggccactct ttttcccctg accttgccca gccggtgaaa cgcctgcgcc acatcggcca
     3601 tctcttccag cgattccaca cggaccgctc cggtctgacg gaaaaaggcc ttccagatct
     3661 tttccccgcc ggccagcgag cccgtgtgcg aagacacggc ccttgctccc gattccgtaa
     3721 ggccgctttt gtaaatgatg accggcttga ggcggttgat tttcttcacc tgctcgagca
     3781 gaagccggcc gtccttcacg ccctcgagat acatggtgat gattccggtt tcccggtcgc
     3841 gggccagata atccagaaaa tccgtgctgt cgagcgtcag ggcgttgccg tagctgatgg
     3901 acttggcaaa gccgatgtcg taaacatccg tcaggtaatt ggaaaaatcc tgggcgttgc
     3961 cgccgctctg gctgatcagg gaaacggggc cggtttttgt ggaagcgttc tcccaggtca
     4021 gcatccggtt tttgggaacg tagaagccca tgcaattcgg cccgatgacg cgcaggcccc
     4081 cttccgcggc gacggtttca atctccttct gcagccggat gccctcttct tcgccggttt
     4141 ccttgaagcc ggagctgaag atatggatat ttttgtgccc cgtggcggcg cattccctga
     4201 gtatgtccgc gacacgaggg gcgggaacgg agatgatgac caggtccgca atttccggaa
     4261 gcgacgacag attcggatag gccctgatcc cgtcgatgtc ctgaagcctg gggttgacgg
     4321 ggtaaagcct tccccggtag ccgaaatttc tcatgcagcc gaacatcccc gaaaatccgt
     4381 tcgcttccgg acgggtggcg ccgaccacgg caacggattt ggcgttcgcc atctcgtcga
     4441 gcgcggcgcg gatttccgcc tgcctttttt gcttcccctt gccgcctgcc gccgcgcccg
     4501 aagccgaggg ggcttcatcc aaaaccacca gcgcgtccac ggccacgatg tttcccttcg
     4561 gggtcgcgat cagcggattg acatccacct cccggatatc cggccgctcc aggccaagcc
     4621 tcgagaggcc cagcagaaca tcggccagtc gctcggaatc aacgggcgct tcgccgcgaa
     4681 aattcccgag cagcttccgg gatgatattt ccccgatcat ccgtttggcc tcttgccggt
     4741 ccagtggcgc aatacgaaaa gccgtgtcgg ccagcgcttc ggtaaaaatg ccgccgaggc
     4801 cgaacatcac cgccggcccg aactggacgt cccggattaa accggcgaca aattcacggc
     4861 tgccggcgat caacggcgaa acgatgcagc cttcccagtc ggcgcccgcg gatgcccgga
     4921 tggcgcgaaa cgcttcgcgg acttcttttg cggatttcag gtttgttttg accagaccgc
     4981 gttcggtttt atgctgtagc tttgcgccgt gccccttgac aaccaccgga tagccggttt
     5041 tttgcgcctg caaaaccgct tctctctcat ttgtggccat ccggtcgcgc gccaccggaa
     5101 cgccatagga cgcaagcagt tttttggatt cggtttccgt catggaacgg cgtccttcgg
     5161 ctctcacacg gcgaatcaga atgtccgcgt tggatggggt catgtttttc cctcaaaatt
     5221 ttttattgta aacagggcat gaaccgttaa cacaaggccg gacgatagtc aacccggacg
     5281 ccgt
//
LOCUS       DAOSRM010000124         5118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_14848_length_5118_cov_91.490026, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000124 DAOSRM010000000
VERSION     DAOSRM010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14848_length_5118_cov_91.490026"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLA58_09630"
     CDS             <1..281
                     /locus_tag="PLA58_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63093.1"
                     /translation="ILALNITLPHLLTTVFLFSAVQKTGKESSSSSVSASPDHANRSL
                     FKPVKKVKMPLVRIMFFVLWTQVIFFDDARKYPMIQVFSKHHEKYFRP"
     gene            306..1598
                     /locus_tag="PLA58_09635"
     CDS             306..1598
                     /locus_tag="PLA58_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOR63094.1"
                     /translation="MASENLETKRWGIAAAAVVIQLCLGTVYAWSVFKIPLMNMHGWD
                     GKTVQYTFMLLMLIIGLSAAFGGTLVDKKGPRFVATIGGILFGIGTLVAGYADQVGSI
                     ALLYLGFGMIAALGNGFGYVTPIATLIRWFPDKRGLVTGLAVMGFGAGAFFMGKIAPA
                     MIAAFKEVDAAGKVISSGVAMTWYIWGVIFLILVTGAAQLFKDPPKGWLPAGFKPAAT
                     SVSAADSFMFGEAVKRPQWWMLWAMLCLNVSAGLGLISQHSPLAQDVYRKTMGLLGDL
                     TPEQIAVVAAAGGTVVAVAAIFNGLGRLFWAKISDNIGRRMVFMIMFATQAVIYLLVA
                     MGYISNYYLFIVVSCYLLACYGGGFATMPAFAADAFGPAYIGKVYGFMLTAWSAAGVI
                     GPFVFEAFKPQALYIAAILLAVGFFIALVYKPPKGKNA"
     gene            1789..2031
                     /locus_tag="PLA58_09640"
     CDS             1789..2031
                     /locus_tag="PLA58_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63095.1"
                     /translation="MARWKYKTSIIDLDRLIPPEAISCNDTGTCVVDGIPGSQDYFKG
                     ILDKEGESEWELIQCQPHGQKLLCIWKKKVAEAFAS"
     gene            2204..3085
                     /locus_tag="PLA58_09645"
     CDS             2204..3085
                     /locus_tag="PLA58_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOR63096.1"
                     /translation="MAFNPPAKMVALVGNAGVGKAKLSVFNLLLRGFMAGAYIAIGAA
                     LCTVCSTGAAPIFGPGAAKLISGAVFPVGLIAIVLTGMELFTGDCMIVPMAAMMKKVT
                     WGDVMRNWVWVYIGNFVGSLAYAYVMVIGPFVTGQPDGSMAVNAFGENAVGIAVAKIL
                     EYKAVGGAGLWSCFIKAIGCNFLVNIAILLAITADDIIGKFFGIWFPIMAFVATGFEH
                     CVANMYFLPAGKFLCDWYPAVIAKKGGLLLANGGLSWGDIWFWNLIPATIGNIFGGFL
                     FIGVAYFLMFKNDLPKD"
     gene            3095..3565
                     /locus_tag="PLA58_09650"
     CDS             3095..3565
                     /locus_tag="PLA58_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOR63097.1"
                     /translation="MSVLTWIVFVICSAAVALHYASTGNAQFLWFAIPGLILLLVIPM
                     TLAWMSRRSFAQAHQQSETKARACKIGKLTPLMIGEVVRISGEVQKISFQWLNRPHFH
                     IQDATGQIRVIMFTAPANKVVVGDKVEAVGVVMKNPLKKTQLAISAISVKRTEK"
     gene            3576..3719
                     /locus_tag="PLA58_09655"
     CDS             3576..3719
                     /locus_tag="PLA58_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63098.1"
                     /translation="MDSREGRVILAALLFGVAPILVSAAGPCAAAIAFTVGLLIGTTA
                     AFA"
     gene            complement(4053..4850)
                     /locus_tag="PLA58_09660"
     CDS             complement(4053..4850)
                     /locus_tag="PLA58_09660"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOR63099.1"
                     /translation="MDMVQTVVLGFVQGLGEFLPISSSAHLVLVPWLMNWAYFGLTFD
                     IALHFGTLIAVVIYFRKDWLKLLHHGFTKPREREGKLFWYLVLATIPGALIGFMLEDA
                     AETIFRQPVLIAGMLMVLGALLYAADRRGKKQIDVENISLRTSFLIGLSQALAIIPGV
                     SRSGVTMTTALALGMTREGAARFSFLLSAPIILGAALVKVPELAANPSVIDAGFLTGM
                     LVACASGLAAIGFLLRYVQTRTFLPFVMYRFAVGALVLGVSFYHGAS"
BASE COUNT         1160 a         1415 c         1391 g         1152 t
ORIGIN      
        1 atatcctggc gcttaatata acacttccgc accttttgac aaccgtcttt ctcttttcag
       61 ccgtgcaaaa gacaggaaag gaaagttcct catcgtccgt ctccgcgtcg cctgatcatg
      121 cgaatcggag cctctttaaa cctgtgaaaa aagttaagat gccccttgtc aggatcatgt
      181 tttttgtgct atggacgcaa gtcatttttt ttgatgatgc gagaaaatat ccgatgattc
      241 aagtattttc aaagcaccac gaaaaatatt tcaggcctta gtaaaggaaa gggaggatgt
      301 cttgtatggc tagtgagaat ctggaaacaa aacgatgggg gatcgcagca gccgcagtgg
      361 tcattcagct ctgcctgggc accgtgtacg cgtggtccgt tttcaaaata cctttgatga
      421 acatgcacgg ctgggacgga aaaacagtgc agtacacctt tatgcttctg atgctgatta
      481 tcggtctgtc ggcggctttt ggcggcacac tggtggataa aaaaggcccc cgcttcgtcg
      541 cgacgatcgg cggcattctt ttcgggatcg gcacgctggt tgccggttac gccgatcagg
      601 tgggaagcat cgctctgctt tatcttggct tcggcatgat cgccgcgctg ggcaatggtt
      661 tcggctatgt gacccccatt gccaccctca ttcgctggtt tccggacaaa cggggcctgg
      721 taacgggtct ggccgttatg ggcttcggcg cgggcgcgtt tttcatgggg aaaattgcgc
      781 cggccatgat cgccgccttc aaggaagttg acgcagccgg caaagtgatt tcttccggcg
      841 ttgccatgac ctggtacatc tggggcgtta ttttcctgat tctcgtcacc ggcgccgcac
      901 agttattcaa agatccgccc aagggatggc ttccggccgg gttcaaaccc gcagccacct
      961 ccgtcagcgc ggcggattcg ttcatgtttg gcgaagcagt gaaaagaccc cagtggtgga
     1021 tgctgtgggc tatgctgtgc ctcaacgttt ccgccggtct gggcttgatt tcccagcatt
     1081 ctccgctggc ccaggatgtt tacagaaaga ccatggggct tctcggcgat ctgacgcctg
     1141 aacagatcgc ggtggtcgcc gccgcaggcg gcacggtcgt tgccgttgcg gctatcttca
     1201 acggattggg aagacttttc tgggcgaaaa tttccgacaa catcggacgc cggatggttt
     1261 tcatgatcat gttcgcaacg caggcagtga tttatctcct ggtggccatg ggctacatct
     1321 ccaactacta tctgttcatc gtggtgtcct gctatctgct ggcctgctac ggcggcggat
     1381 tcgccacgat gcccgccttt gcggcggacg ccttcggccc ggcctatatc ggcaaggttt
     1441 acgggttcat gctgaccgcc tggagcgcgg ccggcgttat cggccccttc gtgttcgaag
     1501 cgttcaaacc tcaggccctg tacattgcag ccatcctgct ggcggtcggt ttcttcatcg
     1561 ccctggtgta caaaccgcca aagggtaaaa acgcctgatc ctgaaatttt cctgacaaaa
     1621 gcccccgatt agtttaatgc tatcgggggc tttttaatgg ccgccccgaa tttaccgacc
     1681 ggcggtcaag attttcttga catcaaagac ccccttccct atactccgcg tggccataaa
     1741 gacttttgct ctcaaaagtc caaatcataa ttctacggag gtatcaccat ggcaagatgg
     1801 aagtacaaaa caagcatcat cgatctggac cgcctgatcc cgcccgaggc catttcctgc
     1861 aacgatacgg gaacctgcgt cgttgacggc ataccgggca gccaggacta tttcaaggga
     1921 attctcgaca aggaaggcga aagcgagtgg gaattgattc aatgccagcc ccacggccag
     1981 aagcttctgt gcatctggaa gaaaaaagtc gcggaagcct tcgccagtta aggagcaaac
     2041 tccgaatcct tcgcatccac gccgattttc cattcaaccc ggcctttcag gcccggcccc
     2101 agcgcgccgg tgcgttgatt tcagcccaac atcatcatgc gaagcagccg tgaaggggat
     2161 ggccccttcg catgaaaatc aagaatacag gaggtaatgt tttatggctt tcaatccccc
     2221 cgcaaaaatg gtcgctctcg tcggaaacgc gggagtcggc aaagctaaac tttccgtttt
     2281 taatctgctg cttcgaggat tcatggcggg cgcctacatc gccatcgggg cggcgctttg
     2341 caccgtctgt tcaaccggag cggcgcccat cttcggcccc ggcgcggcca aactcatcag
     2401 cggcgcggtt ttccccgtcg gcctgatcgc cattgtcctg accgggatgg aactctttac
     2461 cggcgactgc atgatcgtcc ccatggccgc catgatgaaa aaagtcacct ggggcgacgt
     2521 gatgagaaac tgggtctggg tttacatcgg caactttgtc ggctcgctgg cctacgccta
     2581 cgtcatggtc atcggaccct ttgtgacggg acagcccgac ggctccatgg ccgtaaatgc
     2641 cttcggggaa aacgccgtcg gaatcgccgt ggcgaaaatt ctcgaataca aagccgtggg
     2701 cggcgcgggc ctctggtcct gcttcatcaa ggccatcggc tgtaacttcc tcgtcaacat
     2761 cgccattctg ctggccatta cggcggacga catcatcgga aaatttttcg gcatctggtt
     2821 tccgatcatg gcctttgtgg ctaccggctt cgagcactgc gtggccaata tgtattttct
     2881 gccggccgga aaatttcttt gcgactggta tccggccgtc atcgcgaaga agggcggact
     2941 gctgctcgcc aatggaggcc tttcgtgggg cgatatctgg ttctggaacc tgatccccgc
     3001 aacgatcggg aacatcttcg gcggtttcct ctttatcggg gtcgcgtatt ttctgatgtt
     3061 caaaaacgac ctccccaagg attaggatca acgaatgtcc gtcctgacct ggatcgtatt
     3121 tgttatttgt tcagcggcgg ttgccttgca ttacgcctca acggggaacg cgcaattttt
     3181 gtggtttgcc attcccggcc tgattcttct tctggtcatc ccgatgaccc tggcctggat
     3241 gagccgcaga agctttgcgc aggcgcacca gcagtcggaa acgaaagccc gggcctgcaa
     3301 aataggcaaa ctgacgcctt tgatgatcgg agaggttgtt cggatatcgg gtgaggtcca
     3361 aaagatcagt ttccaatggc tcaaccggcc tcactttcac attcaagacg cgacgggaca
     3421 gatacgggtc atcatgttca ccgcacccgc caacaaagtg gttgtcgggg acaaggtgga
     3481 ggctgtcggc gtggtaatga aaaacccgct gaaaaaaaca cagctcgcca tatccgccat
     3541 cagcgtcaaa cgaactgaaa aataaggaga aaaacatgga ttcaagagaa ggaagagtaa
     3601 ttttggcggc ccttttattc ggggtagctc ccattctggt ttcggcggcc ggcccctgcg
     3661 cggcggccat tgcttttacc gtcgggctgc tgatcggaac caccgcggcc tttgcataac
     3721 agcgactacg gcaaacctgt tcgaaaagac tctcccggga caatctcggg ggagtctttt
     3781 tgcattgcat tcaattttaa tgaggtcgta aaaagtcgaa aaatgacaaa tagacggctt
     3841 cgaaaaaagc tccgcaggaa aggcgcgcaa atcctgagga atgatgcgta cttcagtgta
     3901 cgtcgcaata agcccgcccc ggacccggga gcgaaggggc ccccgcacta gtgagtcgat
     3961 gtgtgatgcc aagcctgccc cgaccccggg ggcgaagggc ggggaaggat gcagcgcaac
     4021 gaagcatcag gactttttgg aggccgtcag acttaagacg ccccgtggta aaaagagacg
     4081 ccgagaacca gcgcacccac agcgaagcga tacatcacaa acggcaggaa ggttctggtc
     4141 tgtacgtagc gaagcagaaa gccgatggcg gccaggccgg aggcgcacgc cacaagcatg
     4201 ccggtaagga accccgcgtc tatgacggac ggattggcgg caagctccgg aaccttcacc
     4261 agcgccgctc ccagaatgat cggggccgaa agcaggaagg aaaagcgcgc cgcgccttcg
     4321 cgggtcatcc ccaaagccag cgccgtcgtc atggtcacgc cggagcgcga aacgccggga
     4381 atgatggcca gcgcctgcga caagccgatc agaaagctgg ttcgaagcga aatattttcc
     4441 acatcgattt gtttctttcc ccttcgatcc gccgcgtaaa gaagcgcgcc cagaaccatc
     4501 agcataccgg caattaaaac aggctggcgg aaaatcgttt cggcggcatc ctcgagcata
     4561 aagccgatca gcgcgcctgg aatcgtcgcc agcaccagat accagaaaag cttcccttcc
     4621 ctctctctgg gctttgtaaa cccatgatgc agcagtttga gccagtcctt ccggaaatag
     4681 atgaccacgg cgatcaacgt gccgaaatgc agggcaatgt caaacgtaag gccaaagtat
     4741 gcccagttca tcaaccaggg caccagcacc aggtgcgcgg aactggaaat gggaagaaat
     4801 tctcccagcc cctgaacaaa acccaaaacg actgtttgta ccatatccat gacggccccg
     4861 ttcgatgttc atatttgctg cgttgcctta actgataaat tcgattttgt ccacccctac
     4921 cgggcaggaa acagcggaaa gaagtcctcg cgtccttttg tgataaaaca ggatcctgac
     4981 gggtcaagct tccatccttt tgcttttttt gatccggaag gcttcaaagc gggcggaccg
     5041 agccgcctga tcatttcttt caatctgttg aacatttcat cgaaagaaaa acgccggaaa
     5101 ggttggttta cacggtgt
//
LOCUS       DAOSRM010000125         4826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_16006_length_4826_cov_114.284636, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000125 DAOSRM010000000
VERSION     DAOSRM010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16006_length_4826_cov_114.284636"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..701)
                     /locus_tag="PLA58_09665"
     CDS             complement(114..701)
                     /locus_tag="PLA58_09665"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOR63100.1"
                     /translation="MINAFFVCLTAYLAGSVNFAILFFKIAGRADPRLSFSGNAGTTN
                     VYRLAGMPWAVLVFALDIGRAMAVAALAGYFLDGGWLIWAGFFLVLGNSFPCFHGFRG
                     GKGVANYLGFTVLTVPVAALSAAWLWVLVYWIKRVPFIASFFMVAALSAGHVVSSSGD
                     RLAAAGAVATFALILFNHRKNIREYRRETPPSLPE"
     gene            complement(698..1561)
                     /locus_tag="PLA58_09670"
     CDS             complement(698..1561)
                     /locus_tag="PLA58_09670"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HOR63101.1"
                     /translation="MADLSRSGMKQRFSLEIPIRPYLRDHCVEGKTVFPAVESLAALA
                     GEVRNHCPGAPLNWLKDANFPRMLVLPSDVDAQPAQIEIEEKADGIQASLQTTVTAKN
                     SSIRRTLEHARVIFVRKNALPETAVPFPEAWKLRGAPLFVSADSIYRELVPFGLSYRN
                     IAGNLTVAGDGALAEISGGSGEAEDTLLGSPFVSDAMMHAACVWGQRFAGIVAFPIGF
                     DRRVIHRATKTGEIYFARVTPTDVGSGILRFDAWIFDRQERLCESVCGLRMSDIFRGR
                     LQPPHWIREGI"
     gene            complement(1534..2019)
                     /locus_tag="PLA58_09675"
     CDS             complement(1534..2019)
                     /locus_tag="PLA58_09675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOR63102.1"
                     /translation="MKPKPFLPDAYKADERFVRDRTTGLVWHRTPYRVLYADTDRSQV
                     VYHSNYLRYFEVGRTSLMRDVAYPYKAIEESGYVYPIYDLGLSFYRPLAYDDVMHIHT
                     RPVNLERVRLQFDYVITLAEKGDLVCTGFTKHCAANAAGRPVAVDPKTVRLWQTFPDP
                     V"
     gene            complement(2021..3286)
                     /locus_tag="PLA58_09680"
     CDS             complement(2021..3286)
                     /locus_tag="PLA58_09680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOR63103.1"
                     /translation="MKPPQNRRVFVVGYGAATPLGATFAETWRRAVRGEAGFRKVTRC
                     KVESACDVVGEIPDWFPLELDFTDAKEVYNWNAAFVILTMAVCKEALENAGVTMEASI
                     APRTACLIGSALNGSDAYRAAMHDLEHRGPLRVSPYLLPNLCANLPSGKAGMLLKFTG
                     PIFSPEGACASGNHAIGIGARMIRDGDCDFVLAGGADAPILPELIHGFANMNATIKVH
                     AGDRAAGDPAQASRPFSIDRRGFVLSEGAGVVVLAAEEVIKAHGLEPRAEVLGVGWTS
                     DAHHYTSPNPATIIRAIRETIEDAGLTASDIQYVNAHGTSTAKGDRTEVKCLREIFGR
                     SLEKIPVSSNKSQLGHTLGATAAIEAALAIEGMRQGILLPTINHLPDPEFADIDVVPN
                     KARKQKHEIVLSNAFGFGGTNCCVIFRGV"
     gene            complement(3304..4551)
                     /locus_tag="PLA58_09685"
     CDS             complement(3304..4551)
                     /locus_tag="PLA58_09685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOR63104.1"
                     /translation="MSKKTLILGYDAVSSLGTDMENQWRRAAAGESGIGPLTRFPYGD
                     DFPVRVAGQVEDVDVRPYPFLQPREMAHWTSPIFKYALLVVHRALGKSRIDITPDIAP
                     RVAVTFSSAVGGQDSVLKADRQMIAENKLPHPFANPNSCINMVGGKVSILTGATGPIC
                     ATITACATGVTSMIIGDMMIQQHLADVVIAGAVDFPLVEPILAGFATMNGAYRPREGQ
                     PHEAPEKISRPFSANRRGFVVSEGAGAVILASPDFVRAHGLAASIEMAGFGMTSDASH
                     FVAPNLETVRRCIAVAIADAGLAPADVDAVNAHATSTRIGDKVEADALAQIFGAQVPP
                     TSANKSQLGHAMGASSAIETILAIEGMLRDTVLPTINYEPDPALRIDCVPGTAREVRQ
                     EFVLKNAFGFGGCNACLLLRRIS"
     gene            4612..4758
                     /locus_tag="PLA58_09690"
     CDS             4612..4758
                     /locus_tag="PLA58_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63105.1"
                     /translation="MQELYKNINPDFPLENIFVQVSHHGGPDALAHKPQNQARFFLGT
                     DNSK"
BASE COUNT         1046 a         1506 c         1373 g          901 t
ORIGIN      
        1 cgaaggcgag ttttgtcatt gcctttcgcg agccttagat ttatacaaaa cgctcgcagg
       61 caagcgaaaa gggtatttgg gcaacaggct gtttaggcct gcattgcttc cggttattcg
      121 ggcaaagaag gaggcgtttc ccgacggtac tcccggatat tcttccggtg attgaaaaga
      181 atcagagcaa aggtcgcaac ggcgccggcc gcggcaagcc ggtctccgct ggaagaaacg
      241 acgtgccccg ccgaaagcgc ggccaccatg aaaaaagaag cgataaacgg cacccgcttt
      301 atccagtaaa ccagaaccca gagccacgca gccgaaagcg cggcgacggg tacggtcaga
      361 accgtaaatc ccagatagtt ggccacgcct ttgccgcccc ggaatccatg gaagcaggga
      421 aagctgttgc ccagaaccag aaaaaaaccg gcccagatca gccacccgcc atcaaggaaa
      481 tacccggcca gggccgccac ggccatcgcc cggccgatgt cgagggcaaa aaccagcacc
      541 gcccagggca tgcccgccag acgatagaca tttgtcgttc cggcattgcc gctgaaactc
      601 aggcgcggat cggcgcgtcc tgcaattttg aaaaacagaa tcgcaaaatt gacggacccg
      661 gccagatacg ctgtcagaca aacaaagaaa gcgttgatca tatcccttcc ctgatccagt
      721 gcggcggctg caggcgcccc cggaaaatat cgctcattct caaaccgcaa acgctttcac
      781 acagcctttc ctgacggtca aagatccacg catcaaacct taaaatcccg ctgcctacat
      841 ccgtcggggt gaccctggca aaatagattt ctcccgtctt cgtggcccgg tgaatgactc
      901 tgcgatcaaa accgatcgga aaggccacga tgccggcaaa ccgctgtccc cagacacagg
      961 cggcgtgcat cattgcatcc gacacaaaag gcgaccccag cagcgtatcc tccgcctcgc
     1021 cgcttccgcc cgaaatctcc gccagcgccc cgtccccggc aaccgtcaga tttccggcaa
     1081 tgttccggta ggacaaaccg aaaggaacca attccctgta aatcgaatct gcggaaacga
     1141 aaaggggagc acctcttaat ttccatgcct ctggaaaagg cacagccgtt tccggcaggg
     1201 cattcttcct aacaaagatc acccgcgcgt gctccagcgt cctcctgatg gagctgtttt
     1261 tggcggtcac cgtcgtctgc aacgaagcct ggatgccgtc ggccttctct tcgatttcaa
     1321 tttgcgcggg ctgcgcatcc acatcggacg gcagcaccag cattctgggg aaattcgcgt
     1381 ccttcagcca gttcagcggc gcgccggggc aatggttgcg gacttcaccc gccagcgccg
     1441 cgagggactc cacggcggga aaaacggtct tcccttccac gcaatgatcg cgcaggtacg
     1501 gccggatcgg aatttcgagg gaaaagcgct gcttcatacc ggatcgggaa aggtctgcca
     1561 taagcgaacc gttttcgggt ccaccgccac ggggcgaccg gcggcgttgg ccgcgcagtg
     1621 tttggtgaat cccgtacaga cgagatcccc cttttccgcg agggtaatga cataatcgaa
     1681 ctgaagcctc acgcgctcca ggttgaccgg ccgggtgtgg atgtgcatga cgtcgtcgta
     1741 ggccaggggc cgataaaaag acagtcccag atcgtaaatc ggatacacat agccgctttc
     1801 ctcaatggcc ttgtaggggt atgccacgtc ccgcatcagc gaggtgcgcc cgacctcaaa
     1861 atagcggaga tagttggagt ggtaaacgac ctgcgaacga tccgtatccg cgtacagaac
     1921 gcggtaaggg gtccggtgcc agaccagccc ggtggtccgg tcgcggacaa atcgctcatc
     1981 ggccttgtac gcgtcaggca aaaatggctt gggtttcata tcacactccc cgaaagatca
     2041 cgcagcagtt tgtcccgccg aatccaaacg cgttggacag gacgatttca tgtttttgtt
     2101 tgcgggcctt gttcggcaca acatcgatat cggcaaactc cgggtccggc aggtgattga
     2161 tggtcggcag cagaatcccc tgccgcatgc cttcaatcgc cagcgccgct tcaatggcgg
     2221 ccgtagcccc cagcgtgtgg ccgagctggg atttgttgga ggaaacggga attttctcaa
     2281 gggaacggcc gaagatttcc cgcaggcatt tcacttccgt ccggtcgccc ttcgcggtgg
     2341 acgtgccgtg cgcattgacg tactggatgt cggaggcggt gagccccgcg tcctcaatgg
     2401 tttcccggat cgcccgaata atcgtcgccg gattggggct ggtgtaatgg tgggcgtcgg
     2461 acgtccagcc cacccccagc acttccgccc gcggttcaag cccgtgcgct ttgatcacct
     2521 cttccgcggc cagcacaacg acaccggcgc cttccgaaag gacaaatccc cggcggtcga
     2581 tgctgaacgg acgggaggcc tgcgcgggat cccccgctgc tcggtcgccg gcgtgcacct
     2641 tgatcgtggc attcatgttg gcgaagccgt gaatcagctc gggcagaatc ggcgcgtccg
     2701 ccccgcccgc cagaacaaag tcgcagtctc cgtcgcggat catgcgcgct ccgatgccga
     2761 tcgcgtgatt gcccgacgcg caggcgccct caggcgaaaa aatgggcccc gtaaatttca
     2821 gcagcatgcc cgccttgccc gacggaagat tggcgcacag attcggcaga agataggggc
     2881 tcacgcgcag cggtccccgg tgctccagat cgtgcatggc cgcgcggtac gcgtccgacc
     2941 cgttgagcgc cgatccgatc aggcaggccg tcctcggggc aatggaagcc tccatggtca
     3001 cgcccgcgtt ttcgagcgct tccttgcaga ccgccatcgt taaaatcaca aaagccgcgt
     3061 tccagttata gacttctttg gcgtcggtaa aatccagctc caggggaaac cagtccggga
     3121 tctcccccac gacatcgcag gccgactcca ccttgcagcg ggtgaccttg cgaaaacccg
     3181 cctcgccccg aacggcgcgc cgccaggttt cggcaaacgt cgccccgagc ggcgtggccg
     3241 ccccgtagcc gacgacaaag actcttcggt tctgcggagg tttcatgact tgccttccct
     3301 atcttacgag attcggcgca gcaagagaca cgcattacag ccgccgaatc caaatgcgtt
     3361 cttgagcaca aactcctgcc ggacttccct ggcggtcccg ggcacgcaat cgatccgtag
     3421 ggccgggtcc ggttcgtaat tgatggtggg cagaacggtg tcgcgcagca tgccttcgat
     3481 ggccagaatc gtttcaatgg cggaagacgc gcccatggcg tggccgagct gcgacttgtt
     3541 ggccgaagtg ggcggcactt gtgcgccgaa gatttgcgcg agcgcgtccg cttccacctt
     3601 gtcgccgatt ctggtcgatg tggcatgcgc attgacggca tccacatccg caggcgccag
     3661 ccccgcgtcc gcaatcgcaa cggcgataca gcgtcggacc gtctccagat tgggggcgac
     3721 aaaatggctg gcatcggacg tcatgccgaa tccggccatt tcgatgctcg ccgcaagacc
     3781 gtgcgccctg acaaaatccg gcgacgccag aatgaccgct cccgcgccct cggaaaccac
     3841 aaagccccgg cggttggccg aaaacggccg gctgattttt tccggcgcct cgtggggctg
     3901 gccctccctg ggacgataag cgccgttcat ggtcgcaaat ccggccagaa tcggctcgac
     3961 cagcggaaaa tcgacggcgc cggcaatcac cacgtccgcc agatgctgtt gaatcatcat
     4021 atcgccgatg atcatggagg tgacgccggt ggcgcacgcc gtaatcgtgg cgcagatcgg
     4081 acccgtcgcg cccgtcagaa tggaaacctt gccgcccacc atattgatgc aggaattggg
     4141 attggcaaag ggatgcggca gcttgttttc ggcgatcatc tggcggtccg ccttcagcac
     4201 cgaatcctgt ccgccgacgg ccgagctgaa ggtgacggcc acgcgcggcg cgatgtcggg
     4261 cgtaatgtcg atgcggctct tccccagcgc ccggtgaacg accaggagcg cgtatttgaa
     4321 gatgggcgag gtccagtgcg ccatttcgcg cggctgcaaa aaaggatagg gccgaacatc
     4381 cacgtcttcg acctggcccg caacgcgaac gggaaaatcg tcgccgtagg gaaaccgcgt
     4441 cagagggccg attccgcttt cgccggcggc ggcccgccgc cactggtttt ccatatccgt
     4501 ccccaaagac gaaacggcat cataccccaa gatgagcgtt ttcttggaca tcactcaaat
     4561 cgtatcctaa tccttagacc cttccacagg cattttttac gagcggtcat catgcaagag
     4621 ctttataaaa atatcaaccc agattttcca ttagaaaata tttttgttca agtttcgcat
     4681 catggcggac ctgatgcatt ggcccacaag ccgcaaaacc aggcccgctt ctttctcggg
     4741 acagacaaca gcaagtgact cttgccaggc gcctccagat gagcaagcgt agattcaaga
     4801 aattgtaaaa cgttgttcga actgta
//
LOCUS       DAOSRM010000126         4794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_16136_length_4794_cov_103.813463, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000126 DAOSRM010000000
VERSION     DAOSRM010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16136_length_4794_cov_103.813463"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLA58_09695"
     CDS             complement(<1..425)
                     /locus_tag="PLA58_09695"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63106.1"
                     /translation="MDKPGSLQNGPEPVDFRRKLTVVMILICAALSVLTIRLWYLQII
                     KGDELKQRSENNAVRFRKIQPLRGLIMDHNGVVIVDNRPSFDVLYLPARGVDHEDLIL
                     KLREIYKMKSVDFYPAQPFPRTLKSYLPVRLEKNVGMEKV"
     gene            complement(418..906)
                     /locus_tag="PLA58_09700"
     CDS             complement(418..906)
                     /locus_tag="PLA58_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63107.1"
                     /translation="MMYYVLLPFMAVVLVVLQSKLAEILFSGLLTLEMSLIVVIYAGF
                     RLDLWKGAALSLVVGLAMDCLSGTVLGLFSLLYVCIFLFAWFVSSQIDSERLYLIAGF
                     SLICAMVESLALVLIYQFVFEFDMVGKILPVLVPQTLLVSVLSVCFFYAMRRVERVLK
                     YG"
     gene            complement(903..1748)
                     /gene="mreC"
                     /locus_tag="PLA58_09705"
     CDS             complement(903..1748)
                     /gene="mreC"
                     /locus_tag="PLA58_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOR63108.1"
                     /translation="MFFKNYRAVIFVGAVILAAVFLFSYGLKYDAGQSLLQRLALGAA
                     APVQKLLTLCVESVDNAWTRYIRLVGLEEENRKLKGAIGELQMQLTLYRQGYLEARRL
                     QTLLSLEEREPYRFVAARIIGKEQGALAKTLWIDKGEAHGLKPGMPVMALPGLIGRLT
                     NVSRHSAKVQLLIDEGSNVDVLVERTRQQGMLRGAGSRGCVVRYISKIQDVREGDVIL
                     TSGLSNVFPKGLLVGKVVHTDRSDAGMFLNIRVSPFADFTSLEEVLVLAGERKTPSGE
                     RKAAK"
     gene            complement(1799..2836)
                     /locus_tag="PLA58_09710"
     CDS             complement(1799..2836)
                     /locus_tag="PLA58_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOR63109.1"
                     /translation="MLFDYILGKFSNDLAIDLGTANTLVYVKGKGIVLNEPSVVAVHK
                     NHHGVKKVLAVGNEAKSMLGRTPGNIVAIRPMRDGVIADFDITEAMLKHFILCVHDRK
                     SLVRPRIIVSVPSGITQVERRAVRETVESAGAREVYLIEEPMAAAIGSGLPIAEPTSS
                     MVVDIGGGTTEVAVISLSGIVYAKSVRMAGDKIDEEIVQYMKRKYSLLIGERTAEVIK
                     TEVGCAYPMTEVKTIDVKGRDLISGIPKTIETSSEEIREAIIEPVTLIVDTIKDALEN
                     APPELAGDIVDRGIVLTGGGALLKNLDILVREETGLPVTVADDPLSAVARGAGMALDQ
                     LDVLKEVTIQV"
     gene            3016..4614
                     /locus_tag="PLA58_09715"
     CDS             3016..4614
                     /locus_tag="PLA58_09715"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOR63110.1"
                     /translation="MLKFFRKHARGWFMLVFMGIIIFVFVLYFGTDRSMQTANALAVV
                     DGYVVTESEYYNEYSKMTDMVKARYGGALTADMLKQMDLKKMAYDNLINRQVIIARAK
                     DMKMQVSEDELKQMIMAMPALQTDGKFDNDKYKQLLRYNRMTAEDFEAGQKVSLAAGK
                     IEAIILEGIKASDQEVLDLYTLQNQKINLSFIVVAGSDVAGKIKPTTGDLENYLKKNG
                     NAFRVAEQAKIQYLYFAGNAFAPAEISAADIRDYYNRHKDSYKGKDGKPLAPELAAPM
                     IARDLKQSRGMQEAYLEAKKAHDTIYQEENFEDYAAKHKLKIQTVDFFPINKPPQALA
                     SVKDLAQELASLQRKDISKVLSTDHGYFVIRLDDKKAAYTPQLKNIEADVRQAYLKSE
                     QERLAAQEAESLLEKLRKGESLEKLAAAKGLRIQETGFFQPGNVIPKLGANPDAVNVL
                     MSLSMNRPYPEKPMSFGNSQVILKLKGQSALDMKDFEAKKEIYRKIAMNLKREEAMKS
                     WLEGNKEALIKEKRLKIHKEVKDL"
BASE COUNT         1220 a         1417 c         1225 g          932 t
ORIGIN      
        1 actttttcca tgccgacatt tttctccagc cggacgggaa gataggactt cagcgtcctg
       61 ggaaaaggct gggccgggta aaaatcaacc gatttcatct tgtaaatttc ccggagcttc
      121 aggatcagat cctcatgatc gactccccgg gcaggcaggt acagcacatc aaaggagggg
      181 cggttgtcca cgatcacaac accgttgtgg tccatgatca ggccgcgcag aggctgaatt
      241 ttacgaaaac ggacggcgtt gttttcagag cgctgcttga gttcatcccc tttgatgatc
      301 tgcagatacc acaggcggat cgtcaggacc gacagggccg cgcaaatcag aatcatcacc
      361 acggtaagct tgcgccgaaa gtccaccggc tccggcccgt tttgaaggct gcccggttta
      421 tccatacttc aaaactcttt ccaccctgcg catcgcgtaa aaaaagcaca cagaaagaac
      481 gctgaccagc agggtctgcg gcacaaggac gggaaggatt ttaccgacca tgtcaaattc
      541 aaacacaaac tggtagatca aaaccagcgc cagcgattcc accatggcac agatcaggct
      601 gaagccggcg atcaggtaga gcctttccga atcaatctga gaggaaacaa accaggcaaa
      661 caggaaaatg cagacgtaca acagcgaaaa caaccccagg accgtcccgg acaggcaatc
      721 catcgccagc cccacgacaa gggaaagggc tgctcccttc cacaggtcca ggcgaaaacc
      781 ggcgtagatc accacgatca gcgacatctc cagcgtaagt aggccggaaa aaaggatttc
      841 ggccagcttg gactgcagga ccaccagcac aacggccatg aaaggcaaca gaacataata
      901 catcattttg cggccttcct ttccccggac ggcgtctttc tctcccccgc cagaaccaga
      961 acttcctcca gcgaagtaaa gtcggcaaaa ggagacaccc ggatattcaa aaacatgccg
     1021 gcgtccgaac ggtccgtatg aaccaccttt ccgaccagca gtcccttcgg gaaaacattg
     1081 ctcaggccgg acgtcagaat cacgtcccct tcccgaacat cctgaatctt ggagatgtac
     1141 cggacgacgc agccgcgtga tcccgctccg cgaagcattc cctgctgacg cgtcctttca
     1201 accagaacat ccacgttgga accctcgtcg atcagaagct gaactttggc gctgtgccgg
     1261 gaaacattcg tcagccgtcc gatgaggccg ggaagcgcca tcaccggcat gcccggcttg
     1321 agaccgtgcg cttccccctt gtcgatccag agcgtcttgg ccagagcgcc ctgttccttg
     1381 ccgatgatgc gggccgcgac gaagcggtag ggctcccgct cctcgagcga cagaagcgtc
     1441 tgcagcctcc gggcttccag atacccctgc cggtaaagcg tcaactgcat ctgaagctca
     1501 ccgatggcgc ccttcagttt ccggttctcc tcttccaggc ccaccagacg gatatagcgc
     1561 gtccaggcat tgtcaacgct ttcaacgcac agcgtcagca acttttgaac cggcgccgcc
     1621 gcccccagcg caagcctctg cagcaggctc tggccggcgt cgtatttcag gccgtacgaa
     1681 aaaaggaaaa cggcggcgag gatcaccgct ccgacaaaga tgacggctcg ataattttta
     1741 aaaaacatgc tctacccaaa ctatccgccg ccagtgaaga ccgaacggcc aggcggcatc
     1801 agacctgaat cgtcacctct ttgagcacat cgagctgatc cagcgccatg cccgcgccgc
     1861 gcgccacggc ggagagcgga tcatcggcca ccgtaacggg aagaccggtt tcctcccgca
     1921 ccagaatatc gagattcttc agcagcgctc cgccgccggt cagcacgatg ccgcggtcca
     1981 caatgtctcc ggccagctcc ggcggcgcgt tttccagggc gtccttgatg gtatccacga
     2041 tcagcgtcac cggctcgatg atcgcttccc ggatttcctc cgaactggtt tcgatggtct
     2101 tgggaatgcc ggagatgaga tcgcgacctt tcacgtcaat cgttttcacc tcggtcatcg
     2161 gataggcgca gccgacctcc gtcttgatca cttccgccgt ccgttcgccg atcagcaggc
     2221 tgtatttgcg cttcatgtac tggacaattt cctcgtcgat cttgtcgccg gccatgcgca
     2281 ccgacttggc gtacacgatg ccggacagcg agatgaccgc cacttccgtg gtgccgccgc
     2341 cgatgtcgac caccatggag ctggtgggct cggcaatggg cagccccgac ccgatcgccg
     2401 ccgccatcgg ttcttcgatc agatagactt cgcgggcgcc cgcggattca accgtctcgc
     2461 gcaccgcgcg gcgctccacc tgcgtaatcc ccgacggcac ggaaacaatg atgcgggggc
     2521 gcaccaggga cttgcggtcg tgaacgcaca gaatgaaatg cttgagcatg gcttccgtaa
     2581 tgtcgaaatc cgcgatgacg ccgtcgcgca tgggacggat cgccacaata ttgcccggag
     2641 tgcggcccag catgctcttg gcctcattgc cgaccgccag caccttcttg acgccatgat
     2701 gattcttatg cacggcaacc acggagggtt cgttcaacac gatccccttg cccttgacat
     2761 aaacgagtgt attggcggtt ccgagatcaa tcgccagatc attggaaaac tttcccagaa
     2821 tataatcgaa taacatgagt cctcccgaat tctccttgaa tatatcgtac ttacctacag
     2881 tttggcgtct atatcgtatt tcaacggcct aagcaacgcc tttttaaaat gtatttgcaa
     2941 tttgcccttc tctgtaatac taacggcccc aatgaccccg gttatgagtt ccattaccac
     3001 tttcgaggag acgctatgct gaaatttttc cgcaaacatg cgcgaggctg gttcatgctg
     3061 gtctttatgg gcatcatcat ttttgttttt gtcctgtatt tcggcacgga ccgaagcatg
     3121 cagaccgcca acgctctggc cgtcgttgac ggctacgtgg tcaccgaatc cgaatactac
     3181 aatgaatact ccaaaatgac ggatatggtc aaagcgcgct acggcggcgc gctgacggcg
     3241 gacatgctca agcaaatgga cctgaaaaaa atggcttacg acaacctgat caaccgtcag
     3301 gtcatcatcg ccagggccaa agacatgaag atgcaggtat ccgaagacga actgaagcag
     3361 atgatcatgg ccatgcccgc cctgcagacg gacgggaaat tcgacaacga caaatataag
     3421 cagcttctgc gctacaaccg gatgaccgcc gaagattttg aggccggcca gaaagtcagc
     3481 ctggcggccg gcaaaatcga agccattatt ctcgagggaa tcaaggcctc cgaccaggaa
     3541 gtgctggatc tgtacaccct gcagaaccag aagatcaatc tcagcttcat agttgtcgca
     3601 ggctccgatg tcgcgggaaa aataaaaccg accaccggcg atctggaaaa ttatctcaag
     3661 aaaaacggca atgcgtttcg tgttgccgaa caggccaaaa ttcaatatct gtattttgcg
     3721 ggcaatgcct ttgccccggc cgaaatcagc gccgccgaca tccgggatta ttacaaccgg
     3781 cacaaagaca gttacaaagg caaagacggc aagcccctgg cgccggagct ggccgcgccg
     3841 atgattgccc gggatctgaa gcagagccgc ggcatgcagg aagcctatct ggaagccaaa
     3901 aaagcgcacg acaccatcta ccaggaggaa aacttcgaag actacgcggc aaaacacaaa
     3961 ctgaaaattc agacggttga ttttttcccg atcaacaaac cgccgcaggc gctcgcgtcc
     4021 gtcaaggatc tggcgcagga gctggcctct ctgcagcgaa aagacatcag caaagtcctg
     4081 agcaccgacc atggttactt cgtcatccgc ctggacgaca aaaaagccgc ctacacgccg
     4141 caactcaaga acattgaagc cgacgtccgg caggcctatc tgaaaagcga gcaggaaaga
     4201 ttggctgccc aggaagctga gagcctcctg gaaaaactgc gcaagggcga gagcctggaa
     4261 aagctggctg cggcaaaagg cctgagaatc caggaaaccg gcttcttcca gcccggcaac
     4321 gtcattccca aactgggcgc aaacccggac gccgtcaatg tgctgatgtc cctgtccatg
     4381 aaccgtcctt acccggaaaa gcccatgtct ttcggcaact cgcaggtcat cctcaagctc
     4441 aaaggccaaa gcgcgctgga catgaaggat ttcgaagcca aaaaggaaat ctaccggaaa
     4501 atcgccatga acctgaagcg ggaggaagcg atgaaatcat ggctggaggg aaacaaagag
     4561 gcgctgatca aagaaaaacg attgaaaatt cacaaagaag taaaggattt atagaaggct
     4621 gaatttcggt ttactgcgtt cctgaaaaga agaaaggcgt cattaatccc tactcgcttt
     4681 gctcgcggga taattcgact aacaagcttc agttcactgc gtttctgaaa cttgacaagt
     4741 tcgtaaaaag tcatcttgtc cctcttttgt catgctgagc ctgtcgaagc atga
//
LOCUS       DAOSRM010000127         4771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_16222_length_4771_cov_110.972222, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000127 DAOSRM010000000
VERSION     DAOSRM010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16222_length_4771_cov_110.972222"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PLA58_09720"
     CDS             <1..1016
                     /locus_tag="PLA58_09720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR63111.1"
                     /translation="TVIAQDMPYPTDQYIRIGYTLRMWEYEKEGLDLQQIDILDGDTR
                     ATLFAIGKNELPKIYKDPLPVNPYYPPDKLDHYYVSLQLPVPLGRTIPKIVTHRFHFR
                     NSRTNRDLSLEGATFLPDQAMTPIVIASPVRGKNWLFINQSTMGYHFNAMFFALGKRG
                     TGERFAFDNLQFDDNLKEYFHGNPAKNESYFNYGDPLYAVADGTVVAMRDGLPENSGN
                     DHSVTFKDAIDLAGNYIILDIGKGRYAFYAHCRTHSIRVKAGDRVKEGDEIALLGNSG
                     NSDAPHLHFQICDAPDFFFSRGLPFVLKKYTKIAQGGQDVLPPPKAYENAMMEQFTVI
                     SFE"
     gene            complement(1023..3017)
                     /gene="uvrB"
                     /locus_tag="PLA58_09725"
     CDS             complement(1023..3017)
                     /gene="uvrB"
                     /locus_tag="PLA58_09725"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOR63112.1"
                     /translation="MDTFSLITDFIPSGDQPQAIDRLAEGLARGKEHQVLLGVTGSGK
                     TFTVANVIARAGRPALVIAHNKTLAAQLYEEFKGLFPDNAVEYFVSYYDYYQPEAYLP
                     ATDTYIEKDSAINEEIDKLRHAATHALLTRRDVIIVASVSCIYGLGSPEAYLGMIVQL
                     EVGREIPREDILKRLVEIQYERNDIDFHRGTFRVRGDIVEIFPPYEDEQALRVEFFGD
                     TVEAIHVIDPLRGKKIGSLEQIAVYPGSHYVTTQQNLQRATADIRAELAETLARYKEQ
                     NLLLEAQRLEQRTHFDLEMMEEMGYCQGIENYSRHLTGRKPGEPPPTLMEYLPKDALV
                     IIDESHATLPQLAGMYRGDRSRKETLVKYGFRLPSALDNRPLTFEEYEALPNQRIYIS
                     ATPAQYEITKAQGVRVEQIIRPTGLMDPEIELKPAAAQVDDLLGEIRIRADRGERVLV
                     TTLTKRMAENLTDYYSGLGVRVRYLHSDIHTLERVGIIRDLRLGEFDVLVGVNLLREG
                     LDIPEVSLVAILDADKEGFLRSERSLIQTSGRAARNVGGKVIMYADKITRSIQACLTE
                     TRRRRAIQQKYNEENNITPETIKKSISNILGSIYEADYVTVAVEKKPGTLPVKEEDLP
                     ALIRKLTAEMKQAAKNMEFEKAAELRDEIKELNQILLEMG"
     gene            complement(3019..4233)
                     /locus_tag="PLA58_09730"
     CDS             complement(3019..4233)
                     /locus_tag="PLA58_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63113.1"
                     /translation="MLKKISVPFLGILLFSLPVMAAETHAAEFQNGRFQSLMIQAVGR
                     SFDLDEDGADVLLKKAIALEPENPMGYALEAMLHIFAYEMCFTLEQRRKEKEAIFFYA
                     GEALLRGEKRISVNPRDSQAWLAMALAKVARVHWAIQEKRYFVMARETLNIWNYLEAA
                     KSADPNNYDVDYLTGLLHYHIDHYRGMTGFLSSLLITEGNRQKGLQEIHLAAQKGWIL
                     RDMAQVQLASIYLTYEKQPSHALPIIMELAKKYPDNYSFHFMHALILAEMRRFAEAET
                     LAAQIQKNIASGVPPYARQLEPRYDHLMGRIHFKRGEYGRAESCFQRAIQDKSFYNIR
                     TKARSLLYIGMIHDIRLERKYAEDYYQRVLKVEGGEGSARIEAGQYLKAPYRADGNGS
                     RRESGEKPQGKG"
     gene            complement(4393..4680)
                     /locus_tag="PLA58_09735"
     CDS             complement(4393..4680)
                     /locus_tag="PLA58_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOR63114.1"
                     /translation="MNKQFYVYIMTNKNNTVLYTGVTNDLNRRVYEYKKKLMDGFTKK
                     YNVDKLVFYEVYDDINNAIAREKQIKSGSRVKKIKLIEEMNSGWKDLFNDL"
BASE COUNT         1019 a         1346 c         1207 g         1199 t
ORIGIN      
        1 tcaccgtcat tgctcaggac atgccctacc ccacggatca atatatccgg atcggctata
       61 cattgagaat gtgggaatat gagaaagaag gtctggattt gcagcagatc gacatccttg
      121 acggcgacac ccgggcgacg ctgtttgcca tcggcaaaaa tgaacttccc aaaatctaca
      181 aagacccgct gcccgtcaat ccctattacc cgcccgacaa actagatcat tattacgtca
      241 gtttgcagct tcctgttcct ttggggcgaa ccattccaaa gatcgtgacg caccggtttc
      301 atttccggaa ttcccggacg aatcgggacc tgtcgctgga aggagcaacc ttcctgccgg
      361 atcaggcgat gacgcccatc gtcattgctt cgccggtccg cggcaaaaac tggctgttta
      421 tcaaccagtc caccatgggc tatcatttca acgccatgtt ttttgcactt gggaaaaggg
      481 gaaccggcga gcgcttcgcg tttgacaatt tgcagttcga cgacaatctg aaggaatatt
      541 ttcacgggaa tcccgccaaa aacgaatcct acttcaacta tggggatcct ctgtacgccg
      601 tggcggacgg aacggtcgtg gccatgcgcg acggcctccc ggaaaattcc ggcaatgacc
      661 acagcgtaac cttcaaagat gccattgatc tggccggcaa ttacatcatc ctggatatcg
      721 ggaaaggccg ctatgccttt tacgcgcact gccgcaccca ttcgatccga gtaaaagcag
      781 gagatcgggt aaaagaagga gacgaaatcg ccctgctggg gaattccggc aactccgacg
      841 cgccccacct gcactttcag atctgcgacg cgccggattt ctttttctcc cgcgggctgc
      901 cgttcgttct gaaaaaatac acgaaaattg cgcaaggcgg acaggatgtc ctgccgccgc
      961 ccaaagcgta tgaaaacgcc atgatggagc agtttacggt catcagcttc gagtaggaac
     1021 cgctagccca tctccagcag gatttgattc agctctttga tttcatcgcg cagttcggcg
     1081 gctttctcaa attccatgtt tttggccgcc tgtttcattt ccgccgtgag tttcctgatt
     1141 aacgccggca ggtcttcttc cttcacgggc agcgtccccg gctttttctc cacggcaacc
     1201 gtaacatagt ccgcttcgta aatggatccc agaatgttgc tgatcgactt tttaatggtt
     1261 tcgggcgtga tgttgttttc ttcgttgtac ttttgctgaa tggcccggcg gcggcgggtc
     1321 tcggtaaggc aggcctgaat ggatcgcgtg atcttgtcgg cgtacatgat caccttgccg
     1381 ccgacattgc gcgcggcccg gccgctggtc tggatgagcg accgttcgga gcgcaggaac
     1441 ccttccttgt cggcatccag aatcgccacc agagaaactt cggggatgtc cagcccctcg
     1501 cgcagcaggt tcacgcccac cagcacatcg aattctccca gccggagatc ccggataatg
     1561 ccgacgcgct ccagcgtatg aatgtcggaa tggaggtagc gcacgcggac gcccaggccg
     1621 ctgtaatagt ccgtcagatt ttccgccatg cgcttggtca gcgtcgtcac cagaaccctt
     1681 tcgcctctgt cggcgcggat gcggatttcc ccgagcagat cgtccacctg cgcggccgcg
     1741 ggcttcagtt cgatttccgg gtccattaag cctgtcggac gaatgatctg ctccacgcgg
     1801 acgccctgcg ctttggtgat ttcatactga gccggcgtcg ccgaaatata aatccgctgg
     1861 ttgggcagcg cctcatattc ttcaaacgtg agcggccggt tgtcgagcgc agagggcagg
     1921 cggaagccgt atttcaccag cgtttccttg cgggaccggt ctccccggta catgcccgcg
     1981 agctgcggca gcgtggcgtg gctttcgtca ataatcacca gcgcgtcttt cggcagatac
     2041 tccatcaagg tcggcggcgg ctccccgggc ttgcggccgg tgagatggcg ggaataattt
     2101 tcaatgccct ggcagtagcc catctcttcc atcatctcca gatcaaaatg ggttcgctgc
     2161 tccaggcgct gcgcttccag cagcaggttc tgttccttat atcgggccag tgtttcggca
     2221 agttccgcgc ggatgtcggc cgtcgcccgt tggagattct gctgcgtcgt tacatagtgg
     2281 ctgccgggat agaccgcaat ttgctccagc gaaccgattt tcttcccgcg cagcgggtca
     2341 atgacgtgaa tcgcctcgac cgtatcgccg aagaactcca cgcgcagcgc ctgctcatct
     2401 tcatagggag gaaagatctc cacgatatcg ccccggacgc ggaaggtgcc gcggtgaaaa
     2461 tcgatgtcgt tgcgctcata ctgaatttcc accagacgct tcaaaatatc ctcccgggga
     2521 atttcccggc ccacttcgag ctgaacgatc atccccagat aggcctccgg cgaacccaga
     2581 ccgtaaatgc acgaaacgct cgccacgatg atgacatctc gcctcgtcaa aagcgcgtga
     2641 gtggccgcgt ggcgaagttt gtcgatttcc tcgttgatgg cggaatcctt ttcgatatag
     2701 gtgtccgtcg ccggcaggta ggcttccggc tgatagtaat cgtaatagct gacaaaatat
     2761 tcgacggcat tgtcggggaa aaggcccttg aattcctcgt acaactgcgc ggccagggtt
     2821 ttgttgtgtg caatgacgag cgccggacgg cccgcgcgcg caatcacgtt ggcaacggta
     2881 aaggttttgc cggagccggt caccccgagc agcacctgat gctctttccc gcgagccagc
     2941 ccttcggcca gtctgtcgat cgcctgcggc tgatcgccgg agggaataaa atccgtgatt
     3001 aatgaaaagg tatccacttt accccttccc ttgaggtttc tcgcccgatt cccggcgtga
     3061 tccatttccg tcagcccggt acggcgcttt gaggtattga ccggcttcga tgcgcgccga
     3121 cccttcgccc ccttcaacct tcaaaacccg ctgatagtaa tcttccgcgt atttgcgttc
     3181 cagccggata tcgtgaatca tgccgatgta cagaagcgat ctggcttttg tccggatatt
     3241 gtaaaaggac ttgtcctgaa tcgccctttg aaaacaggat tccgcccttc cgtattcacc
     3301 gcgtttaaaa tggattcgtc ccatcagatg atcgtagcgg ggttccagtt ggcgcgcata
     3361 gggcggcact ccggaagcga tatttttctg gatttgagcg gcaagggttt ccgcttccgc
     3421 gaatcggcgc atctccgcca ggatcagagc gtgcatgaaa tgaaagctgt agttatccgg
     3481 atactttttt gccagttcca tgataatggg caaagcgtgg gacggctgct tttcataggt
     3541 caaatagatt gaggcaagct ggacctgggc catgtcccgc agaatccatc ctttttgcgc
     3601 cgccaggtga atttcctgaa ggcctttctg gcggtttcct tccgtaatca ataacgatga
     3661 aaggaaaccg gtcatcccgc ggtaatgatc aatatggtaa tgcaaaagtc ccgtcaggta
     3721 atccacgtca taattattcg gatcagccga tttggcggct tccagatagt tccagatatt
     3781 cagcgtctcg cgagccatca cgaagtagcg tttttcctga atcgcccagt gaaccctggc
     3841 cactttggcc agcgccatgg ccagccaggc ctgtgagtct cttggattga cgctgattct
     3901 cttttctccc ctcagcagcg cctccccggc atagaaaaag atggcttctt tttctttccg
     3961 gcgctgttca agcgtaaagc acatctcgta ggcaaatatg tgcagcatgg cttccagagc
     4021 gtatcccatg ggattttccg gctccagcgc gatggctttt ttcagcagaa catcggcgcc
     4081 atcctcatcg agatcaaagg accgaccgac ggcctgaatc atcagactct ggaatctgcc
     4141 gttctgaaac tccgcggcat gcgtctccgc agccatgacc ggcagagaga acagcaggat
     4201 acctaaaaaa ggaacggata tttttttcag catatggctt tgatttcctg atgttccttt
     4261 ccgacaatta cgacaacaac gtgaagcaat ctcatggact tttagatcgc cgcgtcatcc
     4321 gcctgcggcg gattcctcgc gatgacgcct tcattagtca ttgcgagccg acccttggtc
     4381 ggcgtggcaa tcttatagat cattgaacag gtctttccaa ccactgttca tttcttcaat
     4441 taattttatc ttctttaccc ttgagccgct tttaatctgt ttttctctgg caatggcatt
     4501 gttaatatca tcataaactt cgtaaaatac taatttatcc acattgtatt ttttcgtaaa
     4561 gccgtccatc aattttttct tgtactcata aactcttctg tttaaatcat tggtaacacc
     4621 ggtatagaga actgtgttgt tcttgttcgt catgatgtaa acgtagaatt gtttattcat
     4681 acaatgtctg ttcacaaatc aaatgcactg agattgcttc gctgcactcg caatgacgat
     4741 ttgaatatct tttccttccg tcttccttct g
//
LOCUS       DAOSRM010000128         4732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_16400_length_4732_cov_110.753902, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000128 DAOSRM010000000
VERSION     DAOSRM010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16400_length_4732_cov_110.753902"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1226)
                     /locus_tag="PLA58_09740"
     CDS             complement(93..1226)
                     /locus_tag="PLA58_09740"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOR63115.1"
                     /translation="MKPAGKNQRRSKLTIGVLYGGRSGEHDVSLCSAASVVAALDKTK
                     YDVVAIGIDRDGRWYVQDRPEIIADKDFGRILSLKKKGAWLVNHFEDRNRLHLYDMDS
                     RGKKVVVDLVFPVLHGTYGEDGTMQGLLELARVPYVGVDVAGSAIGMDKDVAKRLLAQ
                     EGVPVVPWITVSRSDWLARPQKILRGALKRLEMPVFVKPLRTGSSVGIKKVKVKKDLA
                     PAVDYAFGFDTRIMIEKGIDCREIECAVLGNEDPRASVLGEILPTHEYYSYEAKYLDP
                     EGAKMNIPAKIPARLAAAIRKCAVLGFRALNCNSMARVDFFLDKKTGAFYLNEINTLP
                     GFTGISMYPKLWEATGLSYSALLDRLVELALARHREKSAVITG"
     gene            complement(1237..1926)
                     /locus_tag="PLA58_09745"
     CDS             complement(1237..1926)
                     /locus_tag="PLA58_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63116.1"
                     /translation="MSKKTGGANTAIGYRYAEDTLKGDTDRHVVRQRQIYSQAAYGAS
                     GRWEIYGRVGVSDLQIADAFRSSSALTAVSRDSFEENWKFFGTLGAKAYYPVNSVFGV
                     GAFVQGSCNFSNFTDETGGTSGGAPFAADMKVKYLWDVNFGIGVQASVPYGIQLYAGP
                     FVYYTEADAYLSGSVPGIQSGAEKTSLKNASAVGGFFGVAVGLGRGFGLQIEGQYTDR
                     FSAGSAVTYTY"
     gene            complement(2043..3062)
                     /locus_tag="PLA58_09750"
     CDS             complement(2043..3062)
                     /locus_tag="PLA58_09750"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HOR63117.1"
                     /translation="MKVNLFILVLVIFFFAAVGFPGCPAPDAVCARLPEMGNGQVTGD
                     GQWGRGDRLWAMGDGKSQDPVHAESPSAGEDVPARKDMKPSGRSQDAFQDEYKDEGQD
                     YDDEPFADERVTIADPIEPFNRLMHRFNDRLYFWLLKPVAEGYKEAVPEPARISVKNF
                     YYHLLFPIRFVNCVLQADLTGAGSEAGRFAINTVWGVGGLLDPSSNQGIELPKQNADF
                     GQTLGLYGVGHGFYIVWPFLGPSSPRDSVDIAGEYVLDPLSYFTPWYTSLGKRPLKMI
                     NNASLSLGDYEAVIEAAIDPYIAIRNGYIQYRMKEVQARRDRSLFFRNRKNAAPEAAD
                     PPPAN"
     gene            complement(3073..3681)
                     /locus_tag="PLA58_09755"
     CDS             complement(3073..3681)
                     /locus_tag="PLA58_09755"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR63118.1"
                     /translation="MQNRILKKRLIGCSLFLMLLLSIPAYAGAPLSAVENTVNRVLDV
                     LRDPKLKGPTAKPAKTEKLRVIYKDMFDEMEFSRRTLTRNWNKFTPEQRREFVDLFEQ
                     VLEKTYLDRILEYSNEKIVFYKETMIGEDKAEIQSKVIASSKEVPIYYRMILKNGKWK
                     VYDVVVENVSLVQNYRTQFNEILAGGTPEQLLETLRKKVKTH"
     gene            complement(3682..4173)
                     /gene="mlaD"
                     /locus_tag="PLA58_09760"
     CDS             complement(3682..4173)
                     /gene="mlaD"
                     /locus_tag="PLA58_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HOR63119.1"
                     /translation="MKKYKMETMVGIFVVCGLICVGYMTVKLGKLNWFSGDTYPLIAK
                     FTTVTGLKAGNPVNILGIEVGRVERISMDQEDLKAVVEMRIKKDIKIYDDAIASIKTE
                     GLIGDKYLSIDPGGGGDLLGPNGVITDTQAALDIESLISKYAFGEVKKDQSKDQNKEK
                     EKK"
     gene            complement(4178..>4732)
                     /locus_tag="PLA58_09765"
     CDS             complement(4178..>4732)
                     /locus_tag="PLA58_09765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOR63120.1"
                     /translation="LENFNHPFMDEVINSLEGLQKELTGLYSKRQFELLNHLQLRRKG
                     GETFCLVIFSLRELDAIVSQIGHDAAQEILRNMGLFIDKHFGAIGGISTRRATNEILT
                     VLPYSNLAETERIMEDFIADFNRQAVPSLEEGACKFALGGDVELTILAGAAEGQSRTD
                     IDALLEQARSKQKEIGRLQCAPGE"
BASE COUNT          982 a         1388 c         1271 g         1091 t
ORIGIN      
        1 cgagagttcg ctcatgcctt tagactttct cctttacctt tatgaaattc ccttcaaaac
       61 gtctcgatcg aatcctgcgc cctgccaggg tttcaaccgg ttatcacagc cgatttttcc
      121 cggtggcgcg ccagcgcaag ctccaccaga cgatccaaga gagcgctgta ggaaaggccg
      181 gtggcctccc agagcttggg atacatgctg atgccggtaa atccgggcag cgtgttgatt
      241 tcgttgagat aaaacgcgcc ggtcttttta tccaggaaga aatccacgcg cgccatggaa
      301 ttgcagttca gcgcgcggaa gccgaggacg gcgcatttgc ggatggcggc cgccaagcgc
      361 gccgggattt tcgccggaat gttcatcttc gcgccctcgg ggtccagata tttggcctcg
      421 taggaataat actcatgcgt gggcagaatt tcacccagca ccgacgcccg cgggtcttca
      481 ttgcccaaaa cggcgcattc gatctcccgg cagtcgatgc ctttctcgat catgatccgc
      541 gtgtcgaacc caaaggcata atccacggcg ggggcaaggt cttttttgac tttcaccttt
      601 ttgattccga cggaagagcc ggtgcgcagc ggtttgacaa agacgggcat ttcaaggcgc
      661 ttcagcgccc cgcgcagaat tttttgcggc cgggccagcc agtccgaacg gcttaccgtg
      721 atccagggca cgacgggaac cccctcctgc gcgagcagac gcttggcgac gtccttgtcc
      781 atgccgatgg cggaaccggc gacatccacc cccacatagg gaacccgggc cagctccagg
      841 agcccctgca tggttccatc ttctccgtac gtgccgtgga ggacgggaaa gaccagatcc
      901 acgacaacct tctttccgcg ggaatccata tcgtacaggt gcagccggtt tcggtcttca
      961 aaatgattga ccagccaggc gccctttttt ttcaaagaaa gaattctgcc gaagtctttg
     1021 tcggcgatga tttccggccg gtcctgaaca taccagcgtc cgtcgcggtc aatgccgatc
     1081 gcaaccacat cgtatttggt cttgtccagc gccgcaacaa cggaagccgc cgaacaaagc
     1141 gatacgtcat gctcgcccga tcgtccgccg tacaaaacgc cgatggtcaa tttggaccgc
     1201 cgttgattct ttccagcagg cttcatgtgt ttccctctaa tacgtgtagg taacggcgga
     1261 cccggcggag aaccggtccg tgtactggcc ctcgatctgg aggccaaatc ccctgcccag
     1321 cccgacggca acgccgaaaa acccgccgac cgcggatgca ttcttcaaag acgtcttttc
     1381 ggcgccggac tggattcccg gaacggagcc ggataaataa gcgtccgctt ccgtataata
     1441 tacaaaaggc ccggcataca attgaattcc ataagggact gacgcctgaa cgccgatgcc
     1501 gaaattgacg tcccagagat acttcacctt catatcggcc gcaaaagggg ccccgccgct
     1561 ggttccgcca gtttcatcgg tgaaattgct gaagttgcag cttccctgca caaaggcgcc
     1621 gacgccgaag acggaattga ccggataata cgctttggcg cccagtgtcc cgaaaaattt
     1681 ccagttttct tcaaaagagt cccgggagac cgcggtcagg gccgaagaag aacgaaacgc
     1741 gtccgctatc tgcaaatcgg aaacgccgac gcgcccgtaa atctcccagc ggccggaggc
     1801 gccgtacgcc gcctgggaat agatctggcg ctgacgaacc acatggcggt ccgtgtctcc
     1861 cttcaatgta tcttcggcat accggtagcc gatagcagtg ttggcgccgc cggttttttt
     1921 ggacatcgtc tgcggcggcc cgaacatccc gcccgcgagc gcagaagagc aaaagggaag
     1981 ccagatcagg gctatcgtga agagaaaaat ttttttcgta gggtttctcc ttttatgcgg
     2041 tctcagttgg ccggcggcgg atccgccgct tccggcgcag cgtttttccg gttccggaaa
     2101 aagagcgatc tgtccctgcg cgcctggact tccttcatgc gatactggat gtaaccgttg
     2161 cgaatggcga tgtaaggatc gatggccgcc tcgatcaccg cttcgtaatc cccgaggctc
     2221 agggaggcgt tgttgatcat cttgaggggc cgtttgccca ggctggtgta ccagggggtg
     2281 aaataggaaa gcggatccag gacatactcg cccgcgatgt caacactgtc acgcggactc
     2341 gacgggccga gaaacggcca gacaatgtag aagccgtggc ccacgccgta caggcccagc
     2401 gtctgcccga aatcggcgtt ctgcttgggc agttcgatgc cctgattgga ggacggatcc
     2461 aggagccctc ccactcccca caccgtgttg atcgcaaaac ggcccgcttc cgagccggcg
     2521 ccggtcagat cggcctgcag cacacaattg acgaagcgga tgggaaacag caggtggtaa
     2581 taaaaatttt tgacgctgat ccgcgccggt tccggcaccg cctctttgta cccctcggca
     2641 accggcttca gcagccagaa atacaggcgg tcattgaacc ggtgcataag ccggttgaaa
     2701 ggttcgatgg gatcggcgat ggtgacgcgt tcatccgcga aaggctcgtc gtcgtagtcc
     2761 tggccttcat ctttgtattc gtcctgaaag gcatcctgcg accgacccga cggcttcatg
     2821 tccttgcggg caggcacatc ctccccggcc gacggagact ccgcgtgaac gggatcctgg
     2881 cttttcccat cgcccatcgc ccatagccta tcgcctcttc cccattgccc atcgcctgtc
     2941 acctgcccat tgcccatctc cggcaaccgg gcgcagacgg catccggggc cgggcagcca
     3001 gggaagccga cggccgcgaa aaagaaaatg accaggacaa gaatgaacag gttgactttc
     3061 atgggccgaa tgttaatggg ttttcacctt tttgcgcaag gtttcgagga gttgctccgg
     3121 cgtgccgccg gccagaattt cattgaactg ggtgcggtag ttctgcacca ggctcacatt
     3181 ttccacgact acatcgtaaa ctttccactt gccgtttttt aaaatcatcc ggtaatagat
     3241 gggaacttcc ttggaggagg cgatgacctt ggactgaatt tccgccttgt cttccccgat
     3301 catcgtttcc ttgtagaaaa cgattttctc gttggaatat tcgaggattc tatccagata
     3361 ggtcttctcc agcacctgtt caaaaagatc gacaaattct ctgcgctgct ccggggtaaa
     3421 cttgttccag ttgcgggtga gcgtgcgccg ggaaaattcc atctcgtcaa acatgtcttt
     3481 atagatgaca cggagttttt ccgtcttggc cggcttggcc gtagggcctt tcagttttgg
     3541 atcacgcagc acatccaaaa cacggttgac cgtgttttcc acggcgctca gcggcgctcc
     3601 ggcatacgcc ggaatggaca aaagaagcat caggaacaga ctgcacccta tcaacctttt
     3661 tttcaggatt cggttttgca tctatttctt ttccttttct ttattttgat ctttgctctg
     3721 gtcctttttt acttcgccaa aggcgtattt gctgatcagc gattcaatgt ccaaagcggc
     3781 ctgcgtgtcc gtgatgaccc cgttgggccc gagcaggtcc ccgccgccgc ccggatcgat
     3841 gctcagatac ttgtcgccga tgaggccttc cgtcttgatg gaagcaatgg cgtcatcgta
     3901 gattttgatg tcttttttga tccgcatttc tacgacggcc ttgaggtctt cctgatccat
     3961 cgaaatgcgc tccacgcgcc cgacttcaat gcccaggatg ttgaccggat tgcccgcctt
     4021 cagaccggtg acggttgtga atttggcgat gagcggatat gtatctccgc tgaaccagtt
     4081 cagtttcccg agtttgacgg tcatgtatcc cacgcagatc agtccgcaaa caacaaagat
     4141 accgaccatg gtttccatct tgtacttttt catcggttca ttcccccggg gcgcattgca
     4201 atcgcccgat ttctttttgt ttcgatctcg cctgctcaag cagggcgtcg atatcggtcc
     4261 tggattggcc ttcggccgcg ccggccagga tcgtcagctc cacgtcgccc cccagcgcaa
     4321 atttgcaggc cccctcttcc agggagggaa cggcctgccg gttaaaatcc gcgatgaaat
     4381 cttccatgat tctttccgtt tccgcaaggt tggaataggg aagcacggtg agaatctcgt
     4441 tggtggcgcg ccttgtggaa atccctccga tggcgccgaa atgcttgtcg ataaacaacc
     4501 ccatattccg cagaatctcc tgcgccgcgt catggccgat ttgtgagacg atcgcgtcga
     4561 gctcccggag gctgaaaatc accaggcaga aggtttctcc gccctttctt ctgagctgca
     4621 gatggttcag cagttcaaac tgccgcttgg agtaaaggcc ggtcaattcc ttctgcagcc
     4681 cttccagcga gttgatgact tcgtccatga acgggtggtt gaaattttcc ag
//
LOCUS       DAOSRM010000129         4724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_16430_length_4724_cov_74.966160, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000129 DAOSRM010000000
VERSION     DAOSRM010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16430_length_4724_cov_74.966160"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..520
                     /locus_tag="PLA58_09770"
     CDS             110..520
                     /locus_tag="PLA58_09770"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HOR63121.1"
                     /translation="MNKPIYFLVFAAFCLAMLSIPSGSLAFRCGEEVVARGASTAEVL
                     YKCGEPEYITGKKKEVKGTFASGTEFKRSTTYTTGGYQQEEIKTEIWHYNCGDNDFVY
                     ALTFEDGIFVKEETPTRGYGKSKCISAREKSSKR"
     gene            complement(552..1247)
                     /locus_tag="PLA58_09775"
     CDS             complement(552..1247)
                     /locus_tag="PLA58_09775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOR63122.1"
                     /translation="MNDQKGARFMTGSIDREFLNMSTGLYQAARKIARFQNDLKELNL
                     TLEKKVEERTEQIKILSMTDSLTGCFNRAYFMERLPQEIKRAIRYGNAFSIVICDLDH
                     FKKVNDAYGHPGGDQALKEFVLCVKGAFRQDMDWVARYGGEEFVIVLPETNIEGACAL
                     AERIRMAIANRVIPYGGREIRITSSFGITGFDAGASAEKISEEDIIRKADQCLYRAKE
                     GGRNRVVAEGIIR"
     gene            complement(1290..2060)
                     /locus_tag="PLA58_09780"
     CDS             complement(1290..2060)
                     /locus_tag="PLA58_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63123.1"
                     /translation="MMQRLFDRIAKNVCVSFAVAFVSVFGAAAAAPGILLVWATVPYE
                     NPSLFHKEVVVSLILVAIAALIHWNIYGLGRLWGWRCEWKPLRVLNDHLRGGGIPADI
                     PTPTLSEILSLLERLPGMTSRMAAILSAPVVAVMVAVNYVHLGSPLYALYVLRGALVA
                     MITYIMFTYLITEILTYHLRREARFVLAERDALQEKSYSSSLLIKFVFIIILMVASIL
                     ITHGLGRSSITHSALASFAIFAVMNVTVGVFMCVLIFM"
     gene            complement(2172..2582)
                     /locus_tag="PLA58_09785"
     CDS             complement(2172..2582)
                     /locus_tag="PLA58_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63124.1"
                     /translation="MKSLYPRTVARWRLKLIYLALAILLIFNAPAVFAGEELYRSLSA
                     AGKNAESSLFMELYRNGQNQNWPAAKEVQFEGNLRFLDRDAAAGDKIPGIFLVRSLEG
                     ELYIVSIPSDDELKRTGAEAYKKIPPLDTDSNKQ"
     gene            2953..3111
                     /locus_tag="PLA58_09790"
     CDS             2953..3111
                     /locus_tag="PLA58_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63125.1"
                     /translation="MEKIKYWLQEHWDAIMAWYEGLEPLYQYGVLFLLIIAGILIFSF
                     LSLRKVTR"
     gene            complement(3153..>4724)
                     /locus_tag="PLA58_09795"
     CDS             complement(3153..>4724)
                     /locus_tag="PLA58_09795"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOR63126.1"
                     /translation="AVGHRVVHGGAQFPNSVKVDAEVLSSIRKLIPLAPLHNPFNLKG
                     IERMQELVPGAPQVAVFDTSFHANMPEQAYTYALPFAVSEEEQIRRYGFHGTSHKFVT
                     LSTATYLKRPVGELKMISCHLGSGASVCAIDHGRSVDTSMGMTPLEGLIMGTRPGDLD
                     PGVLLHLMRHRSMNAEDLDRMLNKKSGLLGISGTSNDMRTLLKAAESGDFRCEKAINA
                     FCYRVRKYIGAYWAAMGGLDALIFTGGIGENAAEIRARICRGMEALGVVVYDDVNAKM
                     RSRRGQITDISEPGSRIRILVIPADEEKMIARETIHALGRTRSREDLKKLRSSPIAIS
                     VSARHVHLTQEHFERLFGAGRTMTLRSDLSQPGQFAAAEAVNLIGPKGRIDRVRILGP
                     ARKESQVEIARTDQFKLGIEVPIRDSGDTEGTPGLTLEGDAGTVDLEKGVICAKRHIH
                     ISPEEALNLGLRDRDVVRVIVKGIRELIFGDVLVRVNPNYRLDMHLDTDEANAAQITT
                     GTVGYIESIQHRNYM"
BASE COUNT         1079 a         1274 c         1267 g         1104 t
ORIGIN      
        1 ttcacaacaa tcttgacatt ctgtagcagt ttggcgcata aagaagataa cgcactcgat
       61 aacctcaaat aatttttatt cagaaggtaa tattatttgg aggatcacca tgaataaacc
      121 catttatttt ctagttttcg cggcgttttg tcttgcaatg ctatcaatac cctctggcag
      181 cctggccttt cgctgtggcg aagaagttgt tgcccgcggc gcaagcacgg ccgaggtgct
      241 gtataaatgc ggcgaaccgg aatatatcac cgggaaaaag aaagaggtga agggaacatt
      301 cgcctctgga actgaattca agcgatcaac tacctataca accggcggct accagcaaga
      361 ggaaatcaag actgaaattt ggcattataa ttgtggggat aatgactttg tctatgcgct
      421 tacatttgag gatggcatct ttgtcaaaga agaaacgccg acgagaggat atggaaagag
      481 caagtgcata tccgcaaggg agaaatcctc aaaacggtaa gtgagaaaac cggcggctac
      541 cgtaagtgct gtcagcgaat gatgccttcg gcaaccaccc ggttgcgacc tccttctttt
      601 gcccggtaga gacactggtc cgccttgcgg atgatgtcct cctcggaaat tttttccgcg
      661 gacgcgcccg catcgaatcc cgtgatgcca aagcttgacg tgatgcggat ctcccgcccg
      721 ccataaggga tgacccggtt cgcgatggcc atccggattc tttccgccag agcgcaggcg
      781 ccttcaatgt tcgtttcagg caggacgatg acaaattcct cgccgccgta gcgggcaacc
      841 cagtccatat cctgccggaa ggctcctttc acgcacaaaa caaactcttt aagcgcctgg
      901 tcaccccccg ggtggccgta tgcgtcattg acctttttga aatgatcgag atcgcagatc
      961 acaatggaaa atgcattccc ataccggatg gctctcttga tttcctgggg cagacgttcc
     1021 atgaaatagg cgcgattgaa acatcccgtc agagaatcgg tcatcgacag aatcttgatc
     1081 tgttcggttc tctcctcgac tttcttttcc agggtcagat tgagttcttt cagatcgttc
     1141 tgaaatctgg cgatttttcg cgccgcctga taaaggccgg tggacatgtt cagaaattcg
     1201 cggtcgatgc tgccggtcat gaaacgggcg cccttctggt cattcatgtg cagcgccgcc
     1261 gattcgattt ctctcagtgt aatcaagatc tacataaaga tcaggacgca catgaacacg
     1321 cccacggtga cgttcatgac ggcaaaaatc gcgaaagacg ccagagcgga atgagtgatg
     1381 ctgctgcggc ccagtccgtg cgtgatcagg atgctcgcca ccatcagaat gatgataaag
     1441 acaaacttga tcagcaggct cgaggaataa ctcttctcct gaagggcgtc gcgttcggcc
     1501 aggacgaacc gcgcttcccg cctcaaatga taggtaagta tttccgtaat gagatacgtg
     1561 aacataatgt atgtgatcat ggccacaagg gcgccccgca ggacatacag ggcgtataac
     1621 gggctcccca ggtgaacgta gttgacggca accatgacgg cgaccaccgg agcggacaga
     1681 atcgccgcca tccgggaagt catgccggga agcctttcca gaagcgacaa tatttccgaa
     1741 agagtcggcg tcggaatgtc cgccggaatg ccgcctcccc ggagatgatc gttcaggacg
     1801 cgcaggggct tccattcaca gcgccagccc cacagccggc caaggccata gatgttccag
     1861 tgaatcagcg cggcgatggc aaccagaata aggctgacga ccacttcctt gtgaaacaaa
     1921 gagggatttt catacgggac ggtcgcccag accaaaagaa tgcccggcgc ggctgccgca
     1981 gccccgaaga cggaaacaaa agccaccgcg aagctgacgc atacgttttt tgcaattctg
     2041 tcaaacagac gttgcatcat gtcgagtaag cgctttctct cgctccaaag ggccgaattt
     2101 tgccagtgaa gaggggaaag tggaggggag gcgccctcat gtattgacgg atcgcttccc
     2161 ctgcatttca attactgctt gttcgaatcg gtgtctaggg gcggaatttt cttataggct
     2221 tcagcgccgg tccttttgag ttcatcatcg gaagggatcg agacgatata aagttcgcct
     2281 tcgaggcttc tcaccaaaaa aataccgggg atcttgtctc cggcggcggc atccctgtca
     2341 agaaaacgga ggttcccttc aaactgaacc tccttggccg ctggccagtt ctgattttgt
     2401 ccgttgcggt acagctccat gaaaaggctt gattctgcgt tttttccggc ggcggaaaga
     2461 ctgcggtaaa gttcttctcc ggcaaaaacc gccggtgcgt tgaaaatcaa aaggatcgcc
     2521 agcgccaaat aaatcagttt taatcgccat cgggcaaccg ttcgggggta cagactcttc
     2581 atggcaaatc tcctttttca atgagcgctt ccaagcccag cttaacgaga gacgtttttc
     2641 ttaaattccg gcaatattta ccgcttcgtc aatcaagcta ttatctatgt tcaggatggt
     2701 cctctttgat gtctttatat gcattttcca ggcatgcatg tcaaggaatt gcttgattat
     2761 tccaattgct ttctataact tacattatcc atatttttct tgcgtgtcat aaaatcgaac
     2821 ttctcaacgg gtacaaaatt ttaattgcgc ggcaaggggg atttcggtag gaagaaaaag
     2881 ggagaagaca ggattgaaaa gccgctttta aaaaccgcgt ctgctttgca gaaccgttga
     2941 aaggatgggg caatggagaa aataaaatat tggcttcagg aacattggga tgcgatcatg
     3001 gcctggtacg aaggcctgga accgctgtat cagtatggtg ttttatttct gctcatcatt
     3061 gccggtattt tgattttttc gtttttatcg ttaagaaaag tgacccggta gcggctgctc
     3121 aagccttgcg gcagccgttt tcgaggaagg gcctacatgt agttccggtg ctggatggat
     3181 tctatatagc cgacggtgcc ggttgtaatc tgggcggcgt tggcctcatc cgtgtccaga
     3241 tgcatgtcca gccggtagtt tggattgacg cggaccagaa catcgccgaa aatcagctcc
     3301 cgaatgccct tgacgattac cctcaccaca tccctgtcgc gcaggcccag gttcagcgct
     3361 tcttccgggg aaatgtggat atggcgcttc gcgcagatca ctcccttttc cagatcaacg
     3421 gttcccgcgt ctccttccag cgtcaggccc ggcgtccctt ccgtatcgcc ggaatcccgg
     3481 atcggtactt caatgcccag cttgaactga tccgtccggg caatttccac ctgagactcc
     3541 ttgcgcgcgg gccccaggat ccgcacacgg tcgatccttc cttttggccc gatcaggttg
     3601 accgcttccg cagccgcgaa ctgaccgggc tggctcagat cggatcggag cgtcatggtc
     3661 cgtcccgcgc cgaaaagccg ttcaaaatgt tcctgggtca ggtggacatg acgggccgaa
     3721 acggaaatcg caatcgggct ggatcgcagc tttttgaggt cttctctgga gcgtgtccgc
     3781 ccgagggcat ggatcgtttc ccgggcgatc attttttcct catccgccgg aatgacaagg
     3841 atccggattc tggacccggg ctcggaaatg tcggtgatct ggccccggcg ggagcgcatt
     3901 ttggcgttca catcgtcgta aacaacaacg cccagggctt ccatgccccg gcagatgcgg
     3961 gcccggatct ccgcggcgtt ttctccgatg ccgccggtaa agatcagggc gtccagaccg
     4021 cccatggccg cccagtaggc gccgatgtat tttcggacgc ggtagcagaa agcgttgatc
     4081 gctttttcac accggaaatc ccccgactcc gccgccttga gaagcgtcct catgtcgttg
     4141 ctcgttccgg aaatccccag aaggccgctt tttttgttga gcatccggtc caaatcctcg
     4201 gcgttcatgc tccggtggcg catcagatgc aggaggacgc cggggtcgag gtctcccggg
     4261 cgcgtgccca taatcagccc ttccagcgga gtcattccca tgctggtgtc cacgctgcgg
     4321 ccgtggtcaa tcgcacacac cgaagcgccg cttcccagat ggcaggagat cattttcagc
     4381 tcgcctaccg ggcgcttcag ataggttgcc gtgctgagcg tgacgaattt gtggctggtg
     4441 ccgtgaaaac catagcggcg gatctgctct tcctcgctta ccgcgaaagg cagcgcgtac
     4501 gtgtaggcct gctccggcat gttcgcgtga aaggacgtat cgaaaacggc cacctggggc
     4561 gcgccgggaa ccagctcttg cattctctcg attcccttca gattgaaggg attgtgcagc
     4621 ggcgccagcg gaatcagctt ccggatggag gaaaggactt ccgcgtccac cttgacggag
     4681 ttgggaaact gcgctccgcc gtggacgacg cgatgcccca ccgc
//
LOCUS       DAOSRM010000130         4695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_16559_length_4695_cov_112.555172, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000130 DAOSRM010000000
VERSION     DAOSRM010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16559_length_4695_cov_112.555172"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLA58_09800"
     CDS             complement(<1..689)
                     /locus_tag="PLA58_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOR63127.1"
                     /translation="MERLGVLSGTFMMERSDWIRKGQKRRIHNAYGEATAIETDCAVF
                     ILRHGDDPAHYILPHRVNHAANLQALKDLGVTEAIGINSTGSLKTNLRPGMIVIPDDF
                     ISFTPTPTIYENKPIHVTPSFNESVRRKLIAAARKIDLNAVESGIYWQTPGPRLETRA
                     EIRMMSAFADIVGMTLAGEAVIALEMDLPYASACSIDNFGNGLVEKPLSLEEIVAGTR
                     KNADMMIRLVQ"
     gene            1203..1532
                     /gene="trxA"
                     /locus_tag="PLA58_09805"
     CDS             1203..1532
                     /gene="trxA"
                     /locus_tag="PLA58_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOR63128.1"
                     /translation="MSGQFLGVATDANFEQEVLKSDKPVLIDFWAPWCGPCKAIGPLV
                     EEIAGEYANRVKVMKLNVDESQKSAVAFGVRSIPTLILFKDGKVLDTLVGMTPKEKLE
                     EFVKKSL"
     gene            1592..2374
                     /gene="bamD"
                     /locus_tag="PLA58_09810"
     CDS             1592..2374
                     /gene="bamD"
                     /locus_tag="PLA58_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOR63129.1"
                     /translation="MKLKHAGVILAVLLICTGCSFNMAAIKSFFGKSDPVRSTPEGLY
                     SRGTTEYQNGSYKKAREYYTRLKEEYPLHNLAIMAELGIADSFYSDKEYGEAELAYRD
                     FTSLYPTDENVPYALYQTGMCHYVQIGAIDRDQTETIKARREFERLMARYPQSKFSIL
                     AEKMVRECKLKLAEHEFYVGNFYFKNKKYNAALKRFEGIARDYAGVGLDLKVEAYIAE
                     TKERLAKEEQEQKLKEEKEKAKAKKKEEKEKNKVKEKEPANS"
     gene            complement(2512..4104)
                     /locus_tag="PLA58_09815"
     CDS             complement(2512..4104)
                     /locus_tag="PLA58_09815"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR63130.1"
                     /translation="MVHSGIVEQMSRPDFYSHRPQKVEVVQTHISYVFIAGEHVYKVK
                     KPVNFGFLDFTTLEKRKFYCEEELRLNKRLAPAIYLDVVPIGYDVAGRLTLGGTSDIV
                     EYAVFMKKLPLDRMLKILLARGQADAAVMDAVAEKIARFHQEAQTGGRIDEMGAASVI
                     RHNHEENFAQTEKYIDVTLSAFQHAFLKAYAEQFIVSKADLLDKRVAEHKIRDCHGDL
                     HLEHICVADEIIVFDCIEFNERFRFADVAAEVAFLTMDLDYNGYFSQSVNFANAYLKY
                     SRDEDLRALLNFYRCYYAFVRGKVTSFRLDQKEMPQAERAEIRRVASKYFDLACAYAS
                     RLEKPALILTAGLIGSGKSYQARSLAERLGAEVIRTDVLRKELLRIAPNEKHHESFGQ
                     GIYAGDISRKTYEKAVELAAEKIGRGIPVILDASFRCRADRKMAVDMARRLGVPFYVV
                     ECACPDDVVKIRLEKRMRDPDNPSDGRWEILQEQKKQYEAVTEIPESRLFQIDTSDNP
                     ETVRRQIVSSIKMAELRGMIPK"
     gene            complement(4140..4403)
                     /locus_tag="PLA58_09820"
     CDS             complement(4140..4403)
                     /locus_tag="PLA58_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63131.1"
                     /translation="MDPKQIAKQMIVFNKTAFDNNFRAMQALQEQTERLVGRFWEKSP
                     MFPEEGKKAISDWMSAYKKGCDDFKSLVDDNFKKVEDFFKETK"
     gene            complement(4425..>4695)
                     /locus_tag="PLA58_09825"
     CDS             complement(4425..>4695)
                     /locus_tag="PLA58_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63132.1"
                     /translation="QMMEFNKKAFDSSFSAMSELQDQTEKLLMNFLEKADWVPEEGKK
                     AWQDWIVNCKKSRHDFKAAADDGYRKVAEFFEQAEKTQTPAKKKK"
BASE COUNT         1091 a         1201 c         1279 g         1124 t
ORIGIN      
        1 tcctgcacga gacggatcat catatccgcg ttcttgcgcg ttccggcgac gatttcttcc
       61 aggctcaatg gcttttccac cagaccgttt ccgaaattgt cgatggagca ggcggaggcg
      121 taaggcaaat ccatttccag ggcgataacc gcctcgccgg ccagggtcat cccgacgatg
      181 tcggcaaagg cggacatcat ccggatttcc gcccgcgtct ccagacgggg gcccggcgtc
      241 tgccagtaga tgcctgattc cacagcgttg agatcgattt ttcgggccgc cgcaatcagt
      301 ttccggcgga cggattcgtt gaaagaaggg gtgacatgaa tcggcttgtt ctcgtaaata
      361 gtgggagtcg gcgtgaagga gataaaatcg tcggggatga cgatcatgcc cggacgcagg
      421 tttgttttca gggagccggt cgaattgata ccgattgcct cggtgacgcc gagatccttc
      481 agcgcctgaa gattggcggc gtggttgacc cggtggggta aaatgtaatg ggccggatcg
      541 tcgccgtgcc ggaggataaa gactgcacag tccgtttcga tcgccgttgc ctcgccgtaa
      601 gcgttgtgga tccggcgctt ttgacctttc ctgatccagt cgcttcgctc catcatgaat
      661 gttcccgata agacgcccag gcgctccata caaaacctcc cgatgtatgc tttttcatag
      721 cagataaacc gaccgtcgtc aaaaaaatcc gccgccgtgg cagggaacgg ataagtccgg
      781 attacgcaat agcgatatcg taatggtttg aatttaattt tttgtcattt cgaccgaagg
      841 gagaaatctt caacaaacaa ggatgatgaa gatttctctt cgtcccgatt catcggggct
      901 cgtcgaaagg acagctttga ttttttatat cgctattgcg gaatctggga taaaagtaca
      961 tgtagggaac ggtttcaaac cgttttctgg atccccgatc aggtcgggga tgacaaagga
     1021 gatgtagtgc ggggcttcag ccccgctgat agcggacata aatgtccgca ctacataacc
     1081 aaagactgca gggaactgtc gcgaccgttc cctacgtctg gaagcggcga tcaaatagca
     1141 tttgactttt ggaaacccgc tgtgacatat aaccgtgaaa tcaaaaatgg aggttgtaaa
     1201 tcatgagcgg ccaatttctg ggtgtggcga cggatgcaaa ttttgagcag gaagtcctga
     1261 aaagcgacaa acccgtcctc attgatttct gggcgccctg gtgcggaccg tgcaaggcca
     1321 tcggtcctct cgtggaagaa atcgccggcg aatatgcaaa ccgggtcaag gtcatgaagc
     1381 tgaatgtgga cgaaagccag aagtcggccg tcgccttcgg cgttcgcagc attccgaccc
     1441 tgatcctgtt caaggatggg aaggtgctgg acacgctggt cggtatgacg ccgaaggaaa
     1501 aactagaaga gtttgtgaag aaaagtctgt agaggctttc ggcaaagacc gcaaaggcaa
     1561 aatattcaat tccgatagaa cgggtatcct agtgaaacta aaacatgcag gcgtcattct
     1621 cgctgtgctc ttgatctgta ccggctgctc ttttaacatg gcggccatta agtcgttctt
     1681 tgggaagagc gatccggtcc ggtcaacccc ggaagggctc tattcgcggg gcacgaccga
     1741 atatcagaac ggatcttaca aaaaagccag ggaatactat acccgactca aagaggaata
     1801 tcccctgcac aatcttgcga tcatggccga actgggcatc gcggattctt tttacagcga
     1861 caaggaatac ggcgaggcgg agctggccta ccgtgatttc acgtctctgt atcccaccga
     1921 tgaaaatgtt ccctacgcgc tgtaccagac cgggatgtgc cactacgtgc agatcggcgc
     1981 gattgaccgc gaccagacgg aaaccatcaa ggcccgccgg gagttcgagc ggctgatggc
     2041 ccgctatccg cagagcaaat tttccatcct ggccgaaaaa atggtccggg agtgcaaatt
     2101 gaagctggct gaacacgagt tttacgtcgg caacttttat ttcaagaaca agaagtacaa
     2161 tgccgcgctg aagcgttttg aagggatcgc ccgcgattac gccggtgtgg ggctggatct
     2221 gaaagtggag gcctacatcg cggaaaccaa agaacgcctc gccaaagaag agcaggagca
     2281 gaagttgaag gaagaaaaag agaaggccaa ggccaagaaa aaagaagaaa aagaaaaaaa
     2341 caaagtcaaa gaaaaagaac ccgcaaactc ctgacccctc ctattttgac gctgtcgtaa
     2401 aaaattcttc aggccgtcac gtcggcaaaa gacgctctgc cgcaattgcc ttttttattg
     2461 taataatgac caaagaacct gtttgccgga acgcaaaggc aggcgaaaag gctatttggg
     2521 tatcatgccg cgcagttccg ccattttgat ggaggaaaca atttggcggc gcaccgtttc
     2581 cgggttgtcc gatgtgtcga tttgaaagag gcggctttcg ggaatttccg tgacggcttc
     2641 gtattgtttt ttctgctcct gcagaatttc ccagcggccg tcggaagggt tgtccggatc
     2701 ccgcatcctt ttttccagac ggatttttac cacgtcatcg gggcaggcgc attccaccac
     2761 ataaaacgga accccgagcc ttcgcgccat atccacggcc attttccggt cggcgcggca
     2821 tcggaaggag gcgtcaagaa tcaccgggat gccccgcccg attttttcgg cggccagctc
     2881 aacggctttt tcgtaagtct ttcgggaaat gtccccggca tagatgccct gaccgaagga
     2941 ttcatgatgt ttttcgttgg gggcaatccg cagaagctcc ttgcgcagaa cgtccgtgcg
     3001 gatcacttcc gcgccgagtc gctcggccag gctgcgcgcc tgatagctct tgcccgaacc
     3061 gatcagtccc gcggtcagaa tcaaagccgg tttttccagt cgcgacgcgt aggcgcaggc
     3121 gaggtcgaag tattttgagg cgacgcggcg gatctccgcg cgttcggctt gcggcatctc
     3181 tttctgatcc aggcggaagc tcgtgacttt gccgcggacg aaagcgtagt aacagcggta
     3241 aaaattcaaa agcgctcgca ggtcttcgtc gcgggagtat ttcaggtaag cgttcgcgaa
     3301 gttcacggac tggctgaaat agccgttgta atccaggtcc atggtcagga aggcgacctc
     3361 ggccgccaca tccgcgaacc ggaagcgttc gttgaattcg atgcagtcaa aaacaataat
     3421 ttcgtcggcc acgcagatgt gctccagatg caaatcgccg tggcagtctc ggattttatg
     3481 ctccgcgaca cgtttgtcca gcagatcggc tttggagacg ataaattgct ccgcgtacgc
     3541 tttgagaaag gcgtgttgaa aggcggaaag ggtgacgtcg atgtactttt cggtttgcgc
     3601 gaagttttct tcgtggttgt ggcggatcac tgaggcggcg cccatctcgt cgatgcggcc
     3661 gccggtctgg gcctcctgat ggaaccgggc gattttttcg gccacggcgt ccatgacggc
     3721 ggcgtcggcc tggccccggg caagcaaaat tttgagcatt cggtccaggg gcagtttttt
     3781 catgaacacg gcgtattcca cgatgtccga tgttccgccg agtgtcagcc ttcctgcaac
     3841 gtcgtacccg atgggcacga cgtccaggta aatggcgggc gccaggcgtt tgttcagccg
     3901 cagttcttct tcgcagtaaa attttcgctt ttcgagcgtt gtgaaatcaa ggaacccgaa
     3961 attgacaggt tttttgacct tatacacgtg ctcgccggcg atgaagacgt acgaaatgtg
     4021 cgtctgcaca acttcaactt tttggggccg gtgcgaataa aaatccggac gggacatctg
     4081 ttcgacaatg ccggaatgaa ccatgaatac ctcaagaaaa cagacccgcc gaaggtggac
     4141 tatttggttt ccttaaaaaa gtcttccact tttttaaagt tgtcgtccac gaggcttttg
     4201 aagtcgtcgc agcctttttt ataggcggac atccagtcgg agatcgcctt tttcccttct
     4261 tccggaaaca tcggtgattt ttcccagaat cggccgacca gcctttccgt ctgttcctgc
     4321 aaggcctgca tggcgcggaa attgttgtca aaggccgttt tattgaaaac aatcatctgc
     4381 ttggcgattt gtttcggatc catggcaacc tccttgttca aagtttattt tttctttttt
     4441 gcgggcgtct gtgttttttc cgcctgttcg aaaaattcgg cgaccttccg gtagccgtca
     4501 tccgcggcgg ctttaaagtc atgccggctt tttttgcagt ttacaatcca gtcctgccag
     4561 gcctttttgc cttcttccgg cacccaatcc gccttctcca aaaaattcat caacagtttt
     4621 tccgtctggt cctgcagctc cgacatcgcg ctgaaactgc tgtcaaacgc ttttttgttg
     4681 aactccatca tttgt
//
LOCUS       DAOSRM010000131         4595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_17024_length_4595_cov_93.518943, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000131 DAOSRM010000000
VERSION     DAOSRM010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17024_length_4595_cov_93.518943"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..513)
                     /locus_tag="PLA58_09830"
     CDS             complement(349..513)
                     /locus_tag="PLA58_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63133.1"
                     /translation="MTQKSAVSVAGPHTRPVTVMTIPSAKAATNVPLMLPSPPTRTTT
                     TLISRTIDPV"
     gene            complement(510..1643)
                     /locus_tag="PLA58_09835"
     CDS             complement(510..1643)
                     /locus_tag="PLA58_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOR63134.1"
                     /translation="MKRDIAIDYLRSGVTLLVVAHHASLAYNTVSFYDADDYLKSSTP
                     VVDAVRWMPLDFFVGWNDMFFMCLMFLISGLFIIPSLNRKGAGRFMTDRAKRLGIPFI
                     VSVFLLAPLAYYASWLLGSAAGEGGYLSGFFTNNGWASGPAWFIWVLLAYGAVVALVY
                     RLAPGLMKKLSWTASSPRNLVMIFLLASLASTIPLRLFVAPGYWAHIAGPLYFHAWRF
                     LLYFVWFLLGVALGSAEPDRSLSRENLKPWPLWLLLGGITYLFHGVFETQGAGRAIIP
                     GWLSAMILTTLYGLCCTFTGLAALGLARRFFHTARPAADSLTANAYGIYVFHYVFVIW
                     MQFLLLGQPFPAWIKFLIVFITALAASWLLTALLRKTPAGQIL"
     gene            2202..3689
                     /locus_tag="PLA58_09840"
     CDS             2202..3689
                     /locus_tag="PLA58_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOR63135.1"
                     /translation="MRLNSGRLLNERIKTHPRKIAVVAKSEQLDFLTLNQRANRLAHV
                     LAGQGVKCNDKVGILLPNSPEFVVAYLAVQKAGGVTVPLDMKLPPKDVDAILRFADAR
                     LLVTLPSAESIADIKQPMVTVEKEQIKCKGEILHPPDDDWGVDRSAGDEATYLYTSGS
                     TGQPKLAVLTHENLSCFPRVVQEIYATSSEEVYGLLLPMSHVSGPIAIQELIEHGTTL
                     VFLDLMTERRGALQSIQENKVTLIWGVVPLYRLLIHEAKIRKFDTSRLRILAVMGMET
                     PVDFMREMSQAFPHTAVVQGYGLTETSGVVIGTPPRDAVRKMQSIGRPVHFMDVRIVD
                     AKGNPLPPKRHGEIVVKGAAVMKGYYRNEAATKERIKDGWLHTGDVGCLDEEGYLYLL
                     GRTDDMIITGGLNVFPGEVEDVIRRHPKVKDVAVVGLPDPGRGTIVKAVIVPSSEITK
                     EEILKFCRLNLSPFKCPRMVEFRDDLPKTSTGKISRGALRRMEKS"
     gene            3686..4567
                     /locus_tag="PLA58_09845"
     CDS             3686..4567
                     /locus_tag="PLA58_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63136.1"
                     /translation="MNGQTFVKIAKWIFRITPFPVICGLGDGIGRLLFYLMKERTAIG
                     LSNLKRAFPEKPEQERRRILKTFWRNFCKDMLEAIKYFATPSAIMSKRVTLVGQEHLD
                     RLVAQKKGIVLLSAHLGNFPILCMKLGMEGYPIAVLYSEMHNRLFNPVIPLMMRSTGI
                     EPISDRPRHACVGKSMSWLKKGGLLFLQIDQSPPTDAGLPVDFFGWETPTSRGPVSMA
                     MRTDANILPVFIVREKNNHHRIIIDKPFPLKREGDSNRDVLLNLAALSKITEDFIRQY
                     PAHWWWFHRRFKKVINE"
BASE COUNT         1140 a         1140 c         1311 g         1004 t
ORIGIN      
        1 gactcaatcg ttcttcgatt gcgcccgcca taaatctata taacctcata ttttaatggt
       61 tgacggcctc gtaaaaagtc cttttcctct cttttgtcat gcggagcctg tcgaagcatg
      121 acgcgatatc gggcgcttac gctcgccctt cgacgggctc acggtgacag cggtggcttt
      181 ttacgaagcc gtcaagattt gagcgccttg cctgcgcagc tttttacgag gccgtcaatt
      241 gttgcgactg aaaagcggcg tcttactctt gcaaacatca aggaaagatt gagcagccgg
      301 atttatgcca tcgtcaaaga aatgatcgcc gatcacccct gtgcatccct acacgggatc
      361 gatggttctg ctgatcagcg ttgtcgttgt tctcgtcggc gggctgggca acatcaaggg
      421 cacgttcgtg gcggcctttg cgctggggat ggtcatgacg gtcaccggac gcgtatgggg
      481 tccggcgacg gagacggcgg atttttgcgt cacaggattt ggccggccgg ggtctttcgc
      541 aaaagagccg tcaataacca gcttgccgcc agagccgtta taaaaacgat caaaaattta
      601 atccatgccg ggaacggctg ccccagcagc agaaattgca tccagatcac aaagacataa
      661 tgaaagacat aaatgccgta ggcgttggcg gtcaaactgt ccgccgcggg acgggcggta
      721 tggaagaatc gccgggcaag gcccagggcg gcaagccccg tgaatgtgca gcacagacca
      781 tagagggtgg tgagaatcat ggccgaaagc catcccggaa tgatcgcccg gccggcccct
      841 tgagtttcga acacgccatg gaacagatag gttatgccgc ccaggagcag ccacagcggc
      901 cacggcttga gattttcccg cgaaagggac cggtccggct ctgcgcttcc cagagcgacg
      961 cccagcagaa accagacgaa gtacaaaagg aatcgccagg catgaaaata cagggggccg
     1021 gcgatatgcg cccagtagcc gggtgcgaca aacagtcgca gcggaatcgt cgacgcgagg
     1081 ctcgccagaa gaaaaatcat gaccagattc cggggggagg aggccgtcca ggacaacttt
     1141 ttcatgagcc ccggggcgag ccgataaacc aatgcgacga cggcgccata ggccagcagg
     1201 acccagataa accaggcagg cccgctcgcc cagccgttgt tcgtgaaaaa accggacaga
     1261 tagccgccct caccggcggc gctgcctaaa agccatgacg catagtaggc cagcggggcc
     1321 agcaggaaaa cggagacgat aaaagggatg cccaagcgct tggcccggtc ggtcatgaag
     1381 cggcccgcgc ctttccggtt cagggacggg ataatgaaga gcccggagat caggaacatc
     1441 aagcacatga agaacatgtc gttccagccc acgaagaaat ccagcggcat ccagcgaacg
     1501 gcatcgacta cgggggtcga agatttcagg taatcgtcgg cgtcatagaa actgaccgtg
     1561 ttgtaagcca gagaggcgtg atgggcgacg accagcaggg tgacgccgct gcgcaggtag
     1621 tcgatggcaa tgtcgcgttt caaatattcc tccgggcggc cgtcgggttt ggcgaagacc
     1681 ggcggtcgct cgggccgtcg ttgtcgcaat cggtccggcg tgcgccggtt tgaacgcgcc
     1741 gcaatataga taattcgccg ccgcctgtca atgatggaaa cctggaatcg tgatttcaag
     1801 ccgcgacaga gattgttgga atggatggat aatgatcgat attgacaaga gcttcctttg
     1861 gacgggctga acataggagc ggggggttgt cgaggatccg gtccatggtt ttgccgttgt
     1921 gtttttgttg aaaacggagg cgaggggcaa aacgcattta cagcgcttcc cggcttaagg
     1981 gaatgcgctg taactgttgt ggcgatttgt tcatcatcgg aatggatgct ccgcgaagcc
     2041 gggccgagac agggtctgag caggcggatg atttaatttt gggcttgctg aaatggcgaa
     2101 gatgggttat agcgctgccg aaaaaaatca tgtttgtatt tctctttatt caaaaatttt
     2161 gatgatatac aacacaaacg aaaaatggaa ggaactcaaa gatgaggttg aattctggcc
     2221 gattgttgaa tgaaagaata aaaactcatc cccgtaagat cgctgtcgtt gccaagagtg
     2281 agcaattgga ttttctgacg ctgaatcagc gggcaaaccg cctggcccat gttcttgccg
     2341 gacagggtgt aaaatgtaac gataaagttg gcattctgct tcccaattcg cctgaatttg
     2401 tggtcgcgta ccttgccgtt caaaaggcgg gaggggtaac cgttcctctc gatatgaaac
     2461 ttccgccaaa agatgtcgat gcaatcctcc gctttgccga tgcccgcctg cttgttacct
     2521 tgccgtcggc cgaaagcatt gcggatatca agcagccgat ggttacggtg gaaaaggaac
     2581 agattaaatg caaaggggaa attcttcatc caccggatga tgattgggga gtggatcgat
     2641 cagccggtga cgaagcaacc tatctttaca catcgggaag caccggacag cccaaattgg
     2701 ccgttctgac ccatgaaaat ctgagttgtt ttccgcgtgt cgtgcaggag atttatgcca
     2761 cttcttctga agaagtgtac ggactgctcc tgcccatgtc gcacgtgtcg ggaccgatag
     2821 ccattcaaga attgattgaa cacggaacaa cactggtctt ccttgatctg atgacggaaa
     2881 gaaggggcgc cttgcagtcc attcaggaaa ataaggttac cctcatttgg ggggttgtcc
     2941 ccctctatcg gcttcttatc cacgaagcaa aaatcaggaa attcgatacg agccgtttac
     3001 gtattctggc cgtcatggga atggaaaccc ccgtggattt tatgcgtgag atgtcacagg
     3061 catttccgca tacggcggtt gttcaggggt atggtttgac ggaaacttca ggcgttgtca
     3121 tcggaacacc accccgggac gccgtgcgga aaatgcaaag catcggtcgc cccgtccatt
     3181 ttatggatgt tcggatcgtc gatgccaaag gaaatcctct gccgccgaag agacatggtg
     3241 aaatcgtcgt gaagggggca gccgtcatga aaggctatta ccgcaacgaa gcagcaacga
     3301 aagagcggat aaaagacggt tggctccata ccggcgacgt gggctgcctg gatgaagaag
     3361 gttatcttta tctcctgggg cgcacggatg atatgatcat cacgggcggt ctgaatgttt
     3421 tccccggtga ggtggaggac gtgatccggc gacatccgaa ggtcaaggat gtggccgtgg
     3481 tcggcttgcc agaccccggg cggggaacga ttgttaaagc cgtcatcgtt ccttcttccg
     3541 aaatcacaaa agaagaaatt cttaaattct gccgattgaa tctgtctccc tttaaatgtc
     3601 cgcgaatggt cgaattcagg gatgatttgc ccaaaacatc aaccggtaaa atatcccgcg
     3661 gcgccctgcg ccggatggaa aaatcatgaa cggtcaaacc ttcgtcaaaa tcgccaagtg
     3721 gattttcagg atcactccct ttccggtaat ttgcggtttg ggggatggaa tcgggcggct
     3781 tcttttttac ctgatgaagg aaaggacggc tatcggtctt tccaacttaa aacgggcgtt
     3841 tcctgaaaag ccggaacaag agcggagaag aattctaaaa acattttggc ggaatttctg
     3901 taaggacatg ctggaagcaa taaaatattt tgcaacgccg tcggccatta tgagtaaacg
     3961 ggtgacgctt gtcggccaag aacatctcga tcgccttgtc gctcagaaaa aagggattgt
     4021 tcttctgagc gctcacctgg ggaattttcc cattctttgc atgaaactgg ggatggaggg
     4081 ctaccccatt gccgttctct atagcgagat gcacaaccgg ctgttcaatc ccgtcatccc
     4141 gctgatgatg agaagcacgg ggatcgagcc gatttccgac cggccgcggc atgcctgcgt
     4201 gggcaaatcc atgtcctggc ttaaaaaagg gggactgttg tttcttcaga tcgaccaaag
     4261 ccccccgacg gacgcaggac ttccggtgga ttttttcggg tgggaaacac cgacttcccg
     4321 cgggccggtc agcatggcga tgagaacaga cgcaaatatt cttcccgttt tcatcgtgcg
     4381 ggagaagaat aatcatcacc ggatcatcat tgacaaacct ttccctctga aacgcgaggg
     4441 cgacagcaac cgggatgtcc ttctgaatct ggccgccttg agcaagatta cggaggactt
     4501 catccggcaa taccccgccc actggtggtg gtttcatcgt cgatttaaga aagtgatcaa
     4561 cgaataagga aaacacatcc tttccggagg tgttt
//
LOCUS       DAOSRM010000132         4562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_17197_length_4562_cov_111.396716, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000132 DAOSRM010000000
VERSION     DAOSRM010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17197_length_4562_cov_111.396716"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1053
                     /locus_tag="PLA58_09850"
     CDS             205..1053
                     /locus_tag="PLA58_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOR63137.1"
                     /translation="MEYKQVQYKPGKVTQIIFNRPEYLNAQSYQMLAEADQAFTAAAS
                     DQNCGAIVLSGAGRAFSAGHDLGTEEEIQYCREHGLTVPTDGDLPKKVADMHEWFVKK
                     TLDWRNCPKPTVAMVHGYCIYAGWMLAAATDVIFAAEDALFLPGMVEYFSIPWDMGPR
                     KAKEILLEHRFITAAEALAYGFVNRIYPAGQLEKETLAYAERVADNYLMAPAWTGTIK
                     ASINHMQDTMGFSAEMETAYVSFCLMLGLGAYSGAKPDEGGFARTSIARRNFELAKPW
                     LSRRGL"
     gene            1365..2336
                     /gene="rfaD"
                     /locus_tag="PLA58_09855"
     CDS             1365..2336
                     /gene="rfaD"
                     /locus_tag="PLA58_09855"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOR63138.1"
                     /translation="MIIVTGGAGFIGSAFVWKLNCEGIEDVIVVDHLGTSDKWKNLVP
                     LRFEDYLRKDDFFEMVCDDDVPFEVSAVVHMGACSSTTERDADYLWENNFAYTRTLAE
                     WALDHDIRFIYASSAATYGDGTQGFSDEHAKIPELRPINMYGYSKQVFDLWVLRHKLE
                     NQMAGIKFFNVFGPNEYHKGDMTSVIFKAFHQIRQTGKVRLFKSYLPAYPDGGQMRDF
                     VYVKNCVDVLWWLLKHPKTNGIFNLGTGRARTWNDLINAVYAAMNLAPDIEYIEMPEG
                     LRNQYQYFTEADMGKLKKAGAPVDFPALEDSVRDYVQNYLQKPDPHL"
     gene            2407..2646
                     /locus_tag="PLA58_09860"
     CDS             2407..2646
                     /locus_tag="PLA58_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63139.1"
                     /translation="MAQNSRKLNFMIDNDVASELEKLVPAGQRSKVVTQAIVHELALH
                     RRKNITDRLLNLRSQTPKASGKKLLSELAADRQRN"
     gene            2648..3076
                     /locus_tag="PLA58_09865"
     CDS             2648..3076
                     /locus_tag="PLA58_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOR63140.1"
                     /translation="MTSSRLYVPDASVILKWIFDSPKEADREAALALLEDWTKGQCDL
                     LLPSLWIYEVGNIVGRNIPEKAFDFLEMLIEYRIEESPVTAKIAERTLAIMDQCGVTF
                     YDAVYHAVALERRGTLLTADASYLKKAGKLGSVLLLADLY"
     gene            3092..3859
                     /gene="kdsB"
                     /locus_tag="PLA58_09870"
     CDS             3092..3859
                     /gene="kdsB"
                     /locus_tag="PLA58_09870"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOR63141.1"
                     /translation="MKRDIVCIIPSRYESSRFPGKPLADLCGKPMIQHVYERVARAAA
                     VPYVAVATDDQRIFDAVRKFGGNAVMTAATHRSGTDRIAEAVRSLHLSADAIVVNIQG
                     DQPIFEPVQVDEVIAPLVADPAATMSTLIYKIVRPEEISHPHAVKVVFDAECNALYFS
                     RSTIPFVRDKKLVADYYKHHGIYAYRLDFLDVFTRLPEGRLEKLEALEQLRALEYGYK
                     IKVVISPHDSVEVDNEQELDRVRRILQGMESTGSISQ"
     gene            complement(3846..>4562)
                     /locus_tag="PLA58_09875"
     CDS             complement(3846..>4562)
                     /locus_tag="PLA58_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63142.1"
                     /translation="HVSILCSPEMLRHFQAVLAATEEHKDKTYVRSRGYLQVEHAFDP
                     PGTKIPSQMSLVLAPSGTDRKEKQLKIDPFQSRQETGAIAPGRYDVSLCAFGFSTHPD
                     HLTLEVKAGQITDAKFILKPQGMIANLIYAKTALDDRFWGFYRELPGKAKIRSIRLTG
                     QGISRSLTPDDKMSDREVLATFLASRDYAHKNSFAFFDLPAGTYDLTIEADGCETWSE
                     KVKAQPGEFVPPAPIRLIVK"
BASE COUNT         1090 a         1173 c         1265 g         1034 t
ORIGIN      
        1 aatagcctgc cggttttcta taccatatga aaaaactcgg ttgacggata aagttacaga
       61 tcttatccga caccggcgac tgctggacat tcgagatgga gcccgggggg gataaaccgt
      121 ggcgtgtaaa tatcggacag ccggaaagac gaatggtcaa atcacagggg tgaagaacct
      181 gaaaatttaa gaggggggag tctcatggaa tacaaacagg tgcagtataa gcccggcaag
      241 gtaacgcaga tcatctttaa ccggcccgaa tatctgaacg cccagagtta tcaaatgctt
      301 gccgaagccg accaggcctt cacggcagcc gcctccgacc agaattgcgg agcaattgtc
      361 ctgtccggtg cggggcgggc cttttccgca ggccacgatc tggggacgga ggaagaaatc
      421 caatattgcc gggaacacgg cctgacggtg cccactgatg gagacctgcc gaagaaagtg
      481 gcggacatgc acgaatggtt tgtgaaaaaa acattagact ggcgcaactg tccaaaaccg
      541 accgtggcca tggtgcatgg ttattgtatt tatgccggat ggatgctggc cgcggcgacg
      601 gatgtcattt ttgccgccga ggatgctctg tttcttcccg gcatggtcga atacttctcc
      661 atcccctggg acatggggcc gcgcaaggcc aaagaaatcc tcctggagca tcggttcatt
      721 acggcggctg aggcccttgc atatgggttc gtcaatcgga tctaccctgc gggccagctg
      781 gagaaagaga ccctcgccta tgccgaacgc gtggctgaca attacctgat ggctcccgca
      841 tggacaggaa cgatcaaagc atccatcaat cacatgcagg acactatggg attcagcgct
      901 gaaatggaga ccgcctatgt aagcttctgt ctcatgctcg gactcggcgc ctattccgga
      961 gcgaaacctg atgagggtgg ttttgcccgc acaagcattg cccgcaggaa cttcgagttg
     1021 gccaaaccct ggctttccag acgaggactc tgagttgaaa ccattcggat aagatctcgc
     1081 cacaatggcc acaagcactg caaaccaaaa taatggatct gcatcctgct gtttgttgtg
     1141 gcggcgatct tcgccgtcgc atgggatttg aaccatctaa acaaagattt caacaatctg
     1201 tttgcgttgt taagagctgc gcgctcggat gcattctaca aggatatgac catcattgta
     1261 agattcaatg acaaaacggt caccagccgc attttcatca ttgacagccc gccttttcgg
     1321 tattactaca aaatcgtaat ctttgtgtta aggagaaaga aaaaatgatc attgtcaccg
     1381 gcggagccgg atttatcggc agcgcctttg tctggaagct caattgcgaa gggatcgaag
     1441 acgtcatcgt tgtggaccat cttggaacat cggacaagtg gaagaatctg gtccctttac
     1501 ggtttgaaga ctacctccgt aaggacgatt tcttcgagat ggtttgcgat gacgacgtgc
     1561 cgtttgaggt cagcgccgtt gttcacatgg gcgcctgctc ttcgacgacc gagcgcgacg
     1621 cggactacct gtgggagaat aattttgcct acacgcggac gctcgccgaa tgggcgctgg
     1681 atcacgatat ccgcttcatc tacgccagtt ccgccgcgac gtacggcgac ggaacgcagg
     1741 gattttccga cgagcacgca aaaattccgg aactcagacc gatcaacatg tacggctact
     1801 ccaagcaggt tttcgatttg tgggttctgc ggcacaagct ggaaaatcaa atggcgggca
     1861 tcaagttttt caacgtgttc ggccccaatg aatatcacaa gggcgacatg accagcgtca
     1921 ttttcaaagc ctttcaccag atccggcaaa cgggcaaagt ccgccttttc aaatcctatc
     1981 tgccggcgta tcccgacggt ggtcagatgc gggatttcgt gtatgtgaaa aactgcgtgg
     2041 atgtcctgtg gtggctttta aagcatccga agacgaacgg cattttcaat ctgggcacgg
     2101 gcagggccag aacctggaac gacctgatca acgcggttta cgcggcgatg aacctggcgc
     2161 ccgacatcga atacatcgaa atgccggaag ggctgcgcaa tcagtaccag tattttaccg
     2221 aagccgacat gggcaagctc aaaaaagccg gtgctccggt ggactttccc gcgctggaag
     2281 attccgtccg ggattacgtg cagaattacc tgcaaaagcc cgatccgcat ttataaaaag
     2341 agatttgact gcattgtcgc tataatatat acttacaaac gtaacctact tgatggaggt
     2401 atatttatgg cgcagaattc ccggaaactg aatttcatga ttgataacga cgtggcaagc
     2461 gaattggaaa agcttgtgcc cgccgggcaa agaagcaaag tcgtgactca ggcgattgtt
     2521 catgaactgg ccctgcaccg ccgaaaaaac attacggatc gccttcttaa ccttcgatca
     2581 cagacgccta aggcatcagg caaaaaactg ctttctgagt tagccgcaga caggcaaagg
     2641 aattgaaatg acatcttcga gactctatgt tccggatgca tccgtgattc tcaaatggat
     2701 ttttgatagc cccaaagaag cagaccgcga ggcagcgctc gcgctgctcg aggactggac
     2761 gaaggggcaa tgcgaccttt tgcttccgtc cctctggatt tatgaggtcg gtaatattgt
     2821 tgggagaaat attcccgaaa aggcttttga ttttttagag atgcttatcg agtatcggat
     2881 tgaagagtcg cccgtcactg caaagattgc agagcgcact ctggcgatca tggaccaatg
     2941 cggggtgacg ttctatgatg ccgtctatca tgcggttgct ctggaacgaa gaggaacgct
     3001 tttaaccgca gacgcatcgt acctgaaaaa ggccggaaag ctgggaagtg ttcttttgct
     3061 ggctgatctt tattaaccct atggagatag aatgaaaaga gatatcgtct gcatcatccc
     3121 ctcgcgctac gaatccagcc gttttcccgg caagcctctg gccgatcttt gcggcaagcc
     3181 gatgattcag catgtttacg agcgcgtggc ccgggccgcg gccgtgccgt acgtggcggt
     3241 ggccaccgat gatcagcgta tttttgacgc ggtcaggaaa ttcggcggca acgccgtcat
     3301 gacggccgcg acgcaccgct ccggcacgga caggattgcc gaagccgtcc ggtcgcttca
     3361 tctttccgcc gacgccatcg tggtcaacat ccagggcgat cagccgatct ttgaaccggt
     3421 ccaggtggac gaagtgatcg cgccgctggt tgccgatccc gcggcgacca tgtccacgct
     3481 gatttacaaa attgtccggc cggaggaaat atcgcatccg catgcggtca aggtggtgtt
     3541 tgacgctgaa tgcaacgcgc tgtatttttc gcgctcgacg attcccttcg tgcgcgacaa
     3601 aaagcttgtg gcggattact acaagcatca cggaatttat gcatatcgcc ttgattttct
     3661 tgacgtcttt acccgtctgc cggaaggcag actggaaaag ctggaagccc tggagcagct
     3721 tcgggcgctg gaatacggct acaaaatcaa ggtcgtgatt tcgccgcatg attccgtgga
     3781 agtggacaac gagcaggagc ttgaccgcgt tcgccggatc ctgcagggga tggaatcaac
     3841 cggttctatt tcacaataag ccggatgggc gcggggggaa caaactctcc gggctgcgct
     3901 ttcacttttt ccgaccaggt ttcgcagccg tcggcttcga tggtcagatc atacgttccc
     3961 gcaggcaaat caaaaaaagc aaaagagttt ttatgggcgt agtcccggga ggccagaaag
     4021 gtggccagca cttcccggtc gctcatcttg tcgtcgggcg tcagcgaccg ggagatgccc
     4081 tgaccggtta accggatgga gcggattttc gcttttcccg gcaattccct gtaaaatccc
     4141 cagaagcggt cgtccagcgc ggttttggcg taaatgaggt tggcgatcat tccctgcggc
     4201 ttcaggataa atttggcatc cgtaatctgt ccggccttga cctccagcgt caggtgatcc
     4261 ggatgtgtgc tgaacccaaa cgcgcagagg ctgacatcgt aacggccggg cgcgatggcg
     4321 cccgtttcct gccgggattg aaacggatcg attttcaact gtttttcttt ccggtccgtt
     4381 ccggaaggcg ccagcaccag cgacatctgg gaaggaatct ttgtgccggg cggatcgaag
     4441 gcatgctcga cctgcaggta tcccctggag cggacgtacg ttttgtcctt gtgttcttcc
     4501 gtggcggcca ggaccgcctg aaagtgccgg agcatttccg ggctgcacag aatgctgaca
     4561 tg
//
LOCUS       DAOSRM010000133         4509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_17444_length_4509_cov_85.699596, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000133 DAOSRM010000000
VERSION     DAOSRM010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17444_length_4509_cov_85.699596"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /gene="glyA"
                     /locus_tag="PLA58_09880"
     CDS             complement(<1..274)
                     /gene="glyA"
                     /locus_tag="PLA58_09880"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416100.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOR63143.1"
                     /translation="MSFLNQVDPEVAQAIQLETKRQAGKLELIASENFVSEAVLEAQG
                     SVMTNKYAEGYPDKRYYGGCEYVDIPEKLAIERCKKLFGCDEVNVQP"
     gene            complement(299..1540)
                     /gene="fabF"
                     /locus_tag="PLA58_09885"
     CDS             complement(299..1540)
                     /gene="fabF"
                     /locus_tag="PLA58_09885"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOR63144.1"
                     /translation="MKRRVVITGLGAVTPLGNTAAESWANAVAGKSGIGPITRFDASA
                     YASRVAGEIRNFDPLQYVDKKEVRRQDKFAVYALAASQMAMDDAALTLGPDIAERVGV
                     LIGSAIGGAGTFEEEAVVLHASGPRKISPFSVPAILANLASGHVSMRFGAKGPINCAV
                     TACASGTSALGDAYKIIAYGDADAMIAGGVEAAITPLSVAGFCAMRALSLRNDEPEKA
                     SRPFDKGRDGFVIAEGSGVVILEELSFALNRGAKIYAELVGYGCTSDAYHLAAPPPGH
                     EGAGRCMKVAIQDAGLQPADIDYINAHGTSTPLNDLYETQAIKALFGEHAKRLLISST
                     KSMTGHMLGGTGAVEAIFTVKALQEGRLPPTVNLDDPDEECDLDFVPNVARRQDISTA
                     LSNSFGFGGVNAVLVFKKYAP"
     gene            complement(1562..1798)
                     /gene="acpP"
                     /locus_tag="PLA58_09890"
     CDS             complement(1562..1798)
                     /gene="acpP"
                     /locus_tag="PLA58_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOR63145.1"
                     /translation="MSLEEKVIKLVTEQLDVTPEQCKLEASFIDDLGADSLDLVELIM
                     EMEEAFGVEIADEELEKIRTVQDVVDFLRKKGVN"
     gene            complement(1899..2084)
                     /gene="rpmF"
                     /locus_tag="PLA58_09895"
     CDS             complement(1899..2084)
                     /gene="rpmF"
                     /locus_tag="PLA58_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOR63146.1"
                     /translation="MPNPVKRHSKTRRNMRRAHDFLKQPSVSVCPQCGEPKMPHRVCP
                     NCGAYKGREVISAEKMD"
     gene            complement(2105..2647)
                     /locus_tag="PLA58_09900"
     CDS             complement(2105..2647)
                     /locus_tag="PLA58_09900"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOR63147.1"
                     /translation="MSDSLKINLAILPEEGLRLTFSEGSVWFQGCFGGGALPEFSLVS
                     ADVDCLITRSGDTIYVRGDLKAQMRQECSRCLEPATFSAGGAFAYTLVPEKTETAEDL
                     ELSAQELETSYYRGDFIDLAPIICEQIILLAPMKILCTEDCKGLCPHCGVNLNTGSCT
                     CRSKKVDDRLAVLKKFKVIN"
     gene            2813..4216
                     /gene="gltX"
                     /locus_tag="PLA58_09905"
     CDS             2813..4216
                     /gene="gltX"
                     /locus_tag="PLA58_09905"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOR63148.1"
                     /translation="MTAKIRTRFAPSPTGYLHIGGARTALFSWLFARHNGGEFVLRIE
                     DTDQQRSTDESTKAILDAMSWMGLNWDEGPHFQAQRVDLHRQMVQKLLEEGKAYICTC
                     TPEELEIKRKEALATGKKPKYDGACREKGLKKSPGSVVRFRGTDTGVTVVEDLIKGNI
                     VFNNEELDDLIIERGDGYPTYNFAVVIDDAMMNISHVIRGDDHVNNTPRQILMYQALG
                     FEVPKFAHVPMILGSDKTRLSKRHGATSVMAYKEMGYLPEALVNYLVRLSWSHGDQEI
                     FSQKELIEFFSLDAVGKSPAVFNPDKLLWLNAHYIKEASPARLAEEMKPLWPAGADGS
                     DESFIRKIIVDLQPRAKTLVELAQASEFYFAVEISYEAEAAQKFLTPEVAAHLKSIAA
                     AIPGIEDYSKAGLEAFLKNFIEANGIKFKTIAQPLRVALTGKTVSPGIDEVMVTLGKD
                     RVAQRINAAVDYIAKQH"
     gene            4379..4453
                     /locus_tag="PLA58_09910"
     tRNA            4379..4453
                     /locus_tag="PLA58_09910"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4412..4414,aa:Glu,seq:ctc)
BASE COUNT         1029 a         1255 c         1231 g          994 t
ORIGIN      
        1 ggggctggac gttgacttca tcgcagccga aaagcttttt gcagcgctcg atggcgagct
       61 tttcgggaat atccacatat tcacaaccgc cgtaatagcg tttgtccgga tacccttcgg
      121 catatttgtt ggtcattacc gagccctggg cttccaggac cgcttcactg acaaaattct
      181 ccgaggcgat gagttcgagc tttcccgcct ggcgtttggt ttccaactga atggcctgtg
      241 cgacttccgg atcaacctga tttaaaaaag acatgaataa aaaaatcctc caaaaaaact
      301 atggcgcata tttcttgaaa acgagaacgg cattgacgcc gccaaatccg aagctgttgg
      361 acagggcggt ggaaatgtcc tgacgccttg cgacattcgg aacgaaatcc agatcgcatt
      421 cctcgtcggg atcgtccagg ttgacggtgg gcggaaggcg tccctcctga agcgccttga
      481 cggtaaaaat cgcctccacc gcgcccgtgc ctcccagcat atggcccgtc atcgatttgg
      541 tcgaactgat cagcaatctc tttgcgtgct cgccgaaaag cgctttgatg gcttgcgttt
      601 cataaagatc gttcaggggg gtggatgtgc cgtgagcgtt aatgtagtca atatcggccg
      661 gctgcaggcc ggcgtcttgg atcgccactt tcatgcaccg tccggcgcct tcatgccccg
      721 gaggcggcgc ggccaggtga taggcgtccg atgtgcagcc gtatccgacg agttccgcgt
      781 agatctttgc tccccggttg agggcgaaag acagttcttc cagaatgaca acgccgctgc
      841 cttcggcgat gacaaagccg tcgcgtccct tgtcgaaggg gcggctggct ttttccggct
      901 cgtcattgcg gagagaaagc gcgcgcattg cgcagaaccc cgcaacgctg agcggcgtga
      961 tcgcggcttc aacgccgccg gcaatcatgg cgtccgcgtc gccgtaggcg atgattttgt
     1021 acgcgtcgcc gagcgccgac gttccggaag cgcaggccgt cacagcgcaa ttgatggggc
     1081 ctttggcgcc gaagcgcatg gaaacatggc cggacgccag attggccaga atggccggta
     1141 cggagaaagg tgaaattttg cgggggccgg aggcatgaag cacgacggct tcttcttcaa
     1201 aagtgccggc gccgccgatc gcggagccga tgagcacgcc gactcgctcg gcaatgtccg
     1261 ggccgagtgt cagcgccgcg tcgtccatcg ccatctggga cgcggcgaga gcgtaaaccg
     1321 cgaatttgtc ctgccggcga acttcttttt tatcgacata ttgcagcgga tcaaaattcc
     1381 tgatttcacc agcgacgcgg gaggcgtacg cagatgcgtc aaaccgggta atggggccga
     1441 tgccggactt tccggccacg gcgttcgccc aggattccgc tgcggtgttg cccagcggag
     1501 ttacggcgcc gaggcctgtt atcacaacac gccttttcat tgaacaaccc ccgctggaga
     1561 attaattgac gccctttttc cggagaaaat cgacgacgtc ttgaactgtc cggatttttt
     1621 ccagctcctc gtcggcgatt tccacgccga acgcttcttc catttccatg atcagctcaa
     1681 ccagatccag agaatcggcg cccagatcat cgataaaaga agcttccagc ttgcactgct
     1741 ccggtgtgac gtccaactgt tccgtgacga gttttatgac tttttcttct aatgacatgc
     1801 aaccctcctc tagcccaaga ttaggttaaa acgatccaac gcgaaacccc gcctgtcatc
     1861 cgcaatcggc aggggctaaa ccgcctgctc gaacaacatt agtccatctt ttccgccgaa
     1921 attacttccc tgcctttgta cgcgccgcaa ttcgggcaga cacggtgggg cattttcggc
     1981 tccccgcact gcgggcaaac cgacacagaa ggctgtttca ggaaatcgtg cgctctgcgc
     2041 atattgcgtc tggttttaga atgtcttttt actgggtttg gcatagcgta tcctcttgat
     2101 taaattaatt tattactttg aacttcttca gcaccgccag acggtcatca accttttttg
     2161 aacggcaggt gcaggaaccg gtgtttaaat tgacgccgca atgcgggcat aaccctttgc
     2221 aatcctcggt gcagagaatc ttcatcggcg ccagcaggat gatctgttcg cagatgatcg
     2281 gcgccagatc gataaaatcc ccacgatagt agctggtttc cagctcctgt gcggaaagtt
     2341 ccagatcttc cgctgtctcc gtcttttccg ggaccagcgt ataggcaaac gctccgcccg
     2401 cggaaaacgt cgcgggctcc agacagcggc tgcattcctg gcgcatttgc gccttgaggt
     2461 ccccgcggac ataaattgta tcacccgaac gggtgatcaa gcagtccacg tccgcgctga
     2521 ccagtgaaaa ttccggcagc gccccgcccc caaaacatcc ctgaaaccag acgctgcctt
     2581 cggaaaaggt caaccgcagg ccctcttcag ggagaatggc cagattaatt ttcaaagaat
     2641 cagacattga atcttgcagc cctgttataa taagccccac tccaattgag cggggaagta
     2701 aatgataaaa tgattgtatt gtcaagcata atttgcttgc cgaaaggcaa aaggcgcttg
     2761 acaaatgttc cgtcgccgcc ctataacagc ccaaaaattt aagggaatca ttatgaccgc
     2821 caagattcgc acccgctttg ccccgtcccc gaccggctat ctgcacatcg gaggcgcccg
     2881 aaccgccctt tttagctggc tttttgcccg gcataacggc ggtgaatttg tgctgagaat
     2941 tgaagacacg gatcagcagc gctccacaga cgaatccacg aaggcgattc tggacgccat
     3001 gagctggatg ggactcaact gggatgaagg accgcatttt caggcccagc gggtggacct
     3061 gcaccgtcag atggtgcaaa agcttctgga ggaaggcaaa gcctatattt gcacctgtac
     3121 gcccgaagag ctggaaatca aaaggaaaga ggcgcttgcg accgggaaaa aaccaaaata
     3181 tgacggcgcc tgccgcgaga aagggctgaa gaaatcaccc ggctccgttg tccgctttcg
     3241 tggaacggat accggcgtta ccgtcgtgga agacctcatt aaggggaata tcgtctttaa
     3301 taacgaagag ttggatgatt tgatcatcga gcgcggcgat gggtatccca cgtataattt
     3361 tgccgttgtc atcgacgacg cgatgatgaa catttcgcat gtcatccgcg gcgacgacca
     3421 cgtcaacaac accccgcggc agattctgat gtatcaggcg ctgggatttg aagttccgaa
     3481 gttcgcccat gttccgatga ttctgggatc cgacaaaact cgtctgagca agcgccacgg
     3541 cgccacctcc gtcatggcct acaaggaaat gggctatctg ccggaggcgc tcgtcaacta
     3601 cctggttcgc ctgagctggt cgcacggaga ccaggagatt ttttcccaga aagagctgat
     3661 cgagttcttt tcgctcgacg cagtcggcaa gtcgcccgcc gttttcaatc ccgacaaact
     3721 gctgtggctt aacgcccact atatcaagga agcgagtccg gcgaggctgg cggaagaaat
     3781 gaagcccctg tggcccgccg gcgcggacgg aagcgatgaa tccttcatcc ggaagatcat
     3841 cgtggatttg cagccccggg ccaagacgtt ggtggagctg gcgcaggcgt cggaatttta
     3901 ctttgccgtt gagatctctt atgaggcaga agccgcgcag aagtttctga ccccggaagt
     3961 agccgcacat ttgaaatcca tcgccgcggc cattccgggc attgaagatt acagcaaggc
     4021 aggcttggaa gctttcttga aaaactttat cgaggccaac ggcatcaagt tcaaaacgat
     4081 cgcacagccc cttcgggtgg cactgaccgg aaaaaccgtc agccccggca ttgacgaagt
     4141 catggttacg ctggggaaag atcgtgtggc gcaaagaatc aacgcggccg tggactacat
     4201 tgccaaacag cattgagccg gacgcaggaa ggacgcaccg cggcattgaa cggcaatttt
     4261 gccttgacat tttgaggcgc tttgactagg taagcacgct cgactgtccg ctgcgttcgg
     4321 cggattgtcg gttggcggcg accggagttc cgcgccgcgc ggattgaacg catttcatgg
     4381 tcccatcgtc tagtggttag gacgctggcc tctcacgccg gaaaccgggg ttcaaacccc
     4441 cgtgggacta ccaaaataaa aacaattgtt tatgagcatt tcataagcca ttttttattt
     4501 ttaagaacg
//
LOCUS       DAOSRM010000134         4405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_17927_length_4405_cov_89.122069, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000134 DAOSRM010000000
VERSION     DAOSRM010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17927_length_4405_cov_89.122069"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="PLA58_09915"
     CDS             complement(<1..1597)
                     /locus_tag="PLA58_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1010..1012),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOR63149.1"
                     /translation="MQITRRGFLTMTGAVGGGVALSSLGLNLNPTRAYADELGKMDRI
                     KIAKQVTTICCYCSVGCGLVCSVDKATGKIFNIEGDADHPINEGSLCAKGAGFFDLTE
                     ANKHRLTKVLYRKPYGTEWEEKDWDFALSRIARLVKDTRDKGFVKRNDKGELVNRCES
                     IGHYGSSNIDNEECWLVTAKCRALGMVYIDHQARVUHSPSVAALGESFGRGSMTNHYC
                     DLKNADVILIMGSNAAEHHPIAFKWILRAKEKGATIIHVDPRYTRTSARCDLHVPLRS
                     GTDIAFLGGMISYIIENNKYFKDYVLNYTNASFIVGEDYYFKDGLFSGYDAKKRKYDK
                     ATWVLEKDGKGIPKRDMTLTHPRSVFQMMKKHYSRYTLDKVSSITGVSKENLLKVYEI
                     YASTGVPDKAGTECYALGWTHHTTGSQNIRTMSIIQLLLGNMGIAGGGINALRGEPNV
                     QGSTDHAILYNVLPGYLKMPSASLDTLEHYLKKYTPESKDPQSANYYSNYPKFFVSFL
                     KSYWEDKATKENEFGYPWLPKMDD"
     gene            1798..2151
                     /locus_tag="PLA58_09920"
     CDS             1798..2151
                     /locus_tag="PLA58_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOR63150.1"
                     /translation="MEIKYKVWIEKNGKVVFGKGRDDILKAIDEQRSLNAAAKKLEMS
                     YRAAWGRLKASEERMGMKLVEVGVHDKSMQLTGQARSIIDRFEKLEKDVERLLHTADQ
                     DFQKLLQVEEKIGKP"
     gene            complement(2157..2786)
                     /locus_tag="PLA58_09925"
     CDS             complement(2157..2786)
                     /locus_tag="PLA58_09925"
                     /inference="COORDINATES: protein motif:HMM:NF019959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR63151.1"
                     /translation="MSSEERRKKEKESRKNSILKAARKLFFERGFKSVTVDLIAAKAE
                     VSKGSIYLYFDSKEEIYTQILISANIDRHKEWNVFAKQGGTASEALIAFAHEYVSYFL
                     DNNELFRILMTFMLQAENMNLTDEQNTQLIHTTNENVHVIAEILQRGVDSGEFISGMD
                     VRRMQYAVWGLLNGVISLYIYTGVPERRGERIFTIVHDSLNALIKGIKY"
     gene            complement(2942..3532)
                     /locus_tag="PLA58_09930"
     CDS             complement(2942..3532)
                     /locus_tag="PLA58_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOR63152.1"
                     /translation="MKRKIVCALLMMVLLATPAFALSDAYKGNIYEVGKLKPVDSVLK
                     VKVGEIAPGFTLPAVSGRKVSLADYRGKKNVVLSFVPAAWTPVCSDQWPGYNIVQELF
                     EENDAVLLGISVDNIPTLYSWTNQMGHLWFEVLSDFWPHGAVASRLGILRSNGTAERA
                     IIIIDKKGIIRYIDVHDINERPPLESIILQLEKLRK"
     gene            complement(3543..4115)
                     /locus_tag="PLA58_09935"
     CDS             complement(3543..4115)
                     /locus_tag="PLA58_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOR63153.1"
                     /translation="MRKQVAVMMVMMILSLPALLGAETLPPQVGASLPDMKIQKPADS
                     AGLKYLGLSGSGTFSADQVKAQALMIQIFSLYCPYCQKDAPNMNRFFSLMEANPRLRG
                     KIKILGIGAGNTSFEVNTFKKKYKVEFPLVPDADFTIHKIIGEVRTPYFIVVKLGGPK
                     KREVVYSRLGAHEDVEAFLAEVVRLTGIQQ"
     gene            complement(4128..>4405)
                     /locus_tag="PLA58_09940"
     CDS             complement(4128..>4405)
                     /locus_tag="PLA58_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63154.1"
                     /translation="RLIIVGDASMAPYELNDAHGAIYIGQNEARPSVEYLKFLARTFR
                     HAVWLNPQSQDSWFYTWTTKQIAGIFPMFELSLDGLDKAVHRLISRN"
BASE COUNT         1057 a         1139 c         1110 g         1099 t
ORIGIN      
        1 cgtcgtccat cttgggcagc caggggtagc cgaattcatt ttccttcgtt gccttgtcct
       61 cccagtagga tttcaggaag ctgacaaaaa acttcggata attcgaataa tagtttgccg
      121 actggggatc tttggattcc ggcgtgtatt ttttcagata atgttccagc gtgtcgagag
      181 acgccgacgg cattttcaaa tagccgggca gaacattata cagaatcgca tggtcggtgg
      241 aaccctgaac gttcggttcg ccgcgcagag cgttgatgcc gccgcccgca atgcccatgt
      301 tgcccagcag aagttggata atggacatcg tgcggatgtt ctggctgccg gtggtgtggt
      361 gcgtccagcc cagcgcgtag cattcggttc ccgccttgtc cggcacgccg gtggaagcgt
      421 agatttcata aaccttaagg aggttttcct tcgatacgcc ggtgatgctg gaaaccttat
      481 ccagtgtgta gcgggaatag tgttttttca tcatctggaa aacggatcgg ggatgggtca
      541 gggtcatatc ccgtttggga atgcccttgc cgtctttttc cagcacccag gttgccttat
      601 cgtatttgcg tttcttcgca tcgtatccgg agaaaagacc gtctttaaaa tagtagtcct
      661 caccgacgat aaacgaggcg tttgtataat tgagaacata atctttgaag tatttgttat
      721 tctcaatgat gtaggaaatc atgcccccca ggaaagcgat gtccgtgccg gaacgaagcg
      781 gcacatggag atcgcagcgg gcggatgttc gagtgtaacg gggatcaacg tgaataatgg
      841 tggcgccttt ttctttcgct ctcaaaatcc acttgaaagc aatggggtga tgctcggcgg
      901 cattgctgcc cataatcagg atgacatcgg catttttcaa atcacagtag tggttcgtca
      961 tggaaccgcg tccgaacgac tctcccagag ccgctacaga tggactgtgt cagaccctgg
     1021 cctgatggtc gatatagacc atacccagcg cgcggcattt cgcggtcacc agccagcatt
     1081 cttcattatc aatattggag ctgccgtaat ggcctatcga ctcacagcgg ttgaccagtt
     1141 ctcccttgtc atttcttttt acaaaaccct tatcccgcgt gtccttgacc agacgggcaa
     1201 tccgggaaag ggcaaagtcc cagtcctttt cttcccactc cgtaccatac ggcttccggt
     1261 aaagcacctt ggtgagacga tgcttgttcg cttcggtcag atcgaaaaaa cccgcgccct
     1321 tggcgcaaag cgagccttcg ttgatcggat gatccgcgtc gccttcgatg ttgaaaattt
     1381 tccccgtggc cttgtccacg ctgcatacca gaccgcaacc aacggagcaa taacaacaga
     1441 tcgtcgttac ctgcttggca atcttgattc gatccatttt gccaagctcg tcggcatatg
     1501 cccgggttgg attgaggttg agccccagac tcgacagggc cacgcctcct ccaacggctc
     1561 cggtcatcgt taaaaagcct cttcttgtga tttgcataaa aatcctcccc atacatgtgt
     1621 caattttgat ctatgtcata cgtggcatat attgaccgaa agtcatttaa aagtcaatat
     1681 attttctaaa acataatgga gggaaccggc ggggcaaaat gatttgattg catttcacgc
     1741 ccaacttccc tatacagttt atattcatga gtcctgcagg caggaggagt tcttcccatg
     1801 gaaatcaagt acaaggtatg gattgaaaaa aacggaaaag tcgtgttcgg caaggggcgg
     1861 gacgacattc tcaaggccat cgatgagcag cgcagcctca atgccgcggc caaaaagctg
     1921 gaaatgtcct atcgggcggc atggggacga ttgaaagctt cggaagagcg gatgggcatg
     1981 aagctggtgg aagtcggcgt gcacgacaaa tccatgcagc tcaccgggca ggcccgatcc
     2041 attattgatc gatttgaaaa actggaaaaa gacgtggaac ggcttctgca cacggcagac
     2101 caggattttc agaaattact ccaggtggaa gagaaaatcg gcaaacccta aggattttaa
     2161 tattttatgc ctttgatcaa agcgttgagg ctgtcgtgaa cgatggtgaa gattcgctcc
     2221 cctcttcttt caggcacgcc ggtatagatg taaagagaaa tcactccgtt gagaagcccc
     2281 cagacggcat actgcattcg gcggacgtcc atgccggaaa tgaattcgcc tgaatccacg
     2341 ccccgttgaa gaatttccgc aatcacgtgc acattttcat tggtcgtgtg aatgagctgt
     2401 gtattttgct catccgtcag attcatgttt tccgcctgca gcataaaggt catcaggatc
     2461 cggaacaact cgttattatc cagaaagtag ctgacgtatt catgggcgaa agcgatcagg
     2521 gcctccgaag cggttcctcc ctgtttggca aaaacgttcc attccttgtg acgatcgata
     2581 ttggctgaaa tcaggatctg ggtgtaaatt tcttccttgc tgtcaaaata gaggtaaatg
     2641 gagcccttgc tcacctccgc cttggcggcg atcaggtcaa ccgtgacgga tttgaacccc
     2701 cgttcaaaaa acagcttgcg ggcggccttc aaaatggaat ttttccggct ctccttttct
     2761 ttttttcttc tttcttccga actcaagatc ggctctcctt tgaacgcaag aggaagaccc
     2821 cctcggcgtc tttttgcaag tctctgttaa tgcttgataa atgctaacag aatggcctca
     2881 tgacttacgg tcatgttgcg taccgcaatt gaaaacccct gtcaaggaaa attaaagaaa
     2941 tctatttgcg cagtttttca agctgcagga taatgctttc cagaggcgga cgctcgttga
     3001 tgtcatgaac atcaatgtag cggatgatgc cttttttatc gatgatgatg attgcccgct
     3061 ccgccgttcc gttggaacgc agaatgccca agcgggatgc taccgcgccg tgcggccaga
     3121 aatcggacag cacctcaaac cagagatgcc ccatctgatt ggtccaggaa tagagagtcg
     3181 gaatattatc gacgctgatg cccaggagca cggcgtcgtt ttcttcaaac agttcctgaa
     3241 cgatattata gccgggccac tggtcggaac aaaccggcgt ccaggccgcc ggcacaaagg
     3301 acagcacgac attctttttg ccccggtagt ctgcgagcga caccttcctg ccgcttacgg
     3361 ccggcagcgt gaaaccgggg gcgatctctc cgactttgac ttttaaaaca ctgtccaccg
     3421 gcttcagctt gccgacctca taaatgttcc ccttgtaagc gtcggaaagg gcaaaagccg
     3481 gagttgccag gagaaccatc atcaacaacg cgcagacaat ctttcttttc atggaagcct
     3541 cctcactgct gaattccggt caaccggacc acttcggcca gaaacgcttc cacgtcttcg
     3601 tgcgcgccca gcctggagta aaccacttcc cgttttttgg gcccgcccag tttcacaaca
     3661 ataaaatacg gcgtgcggac ttcgccgatg attttgtgaa tggtaaaatc cgcgtccgga
     3721 accagcggaa attcaacctt gtacttcttt ttgaacgtat tcacttcaaa ggatgtgttt
     3781 cccgcgccga tgccgagaat cttgattttg cccctgagcc gcggattggc ctccatcaga
     3841 gagaaaaagc ggttcatatt gggcgcatcc ttctgacagt aggggcaata gaggctgaag
     3901 atctggatca tcaaagcctg tgctttgacc tgatccgcgg aaaacgtacc ggagccggaa
     3961 aggcccagat atttgagacc ggccgaatcc gcgggttttt gaattttcat atcggggagc
     4021 gacgctccca cctgcggcgg cagcgtttca gcgcccagca gtgccggcag cgataaaatc
     4081 atcatgacca tcatcacggc gacttgtttt ctcatgtttt ctcctcctca atttcgggat
     4141 atcaggcgat gcacggcttt atccaatccg tccagcgaca gttcgaacat cggaaaaatg
     4201 ccggcaatct gtttggtcgt ccaggtataa aaccaggaat cctgcgactg cggattgagc
     4261 catacggcgt ggcgaaacgt cctggccaga aacttcagat attccacgga aggccgggct
     4321 tcgttctggc cgatataaat tgcgccgtgg gcgtcattga gttcataggg cgccatggac
     4381 gcgtccccca cgataatcag ccgcg
//
LOCUS       DAOSRM010000135         4300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_18493_length_4300_cov_113.383274, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000135 DAOSRM010000000
VERSION     DAOSRM010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18493_length_4300_cov_113.383274"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..727)
                     /locus_tag="PLA58_09945"
     CDS             complement(29..727)
                     /locus_tag="PLA58_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HOR63155.1"
                     /translation="MIHIEQFRYGDNLAYLLYGKTEAMAIDGGAWQEILSFLERHTLS
                     LRYVANTHRHYDHTPGDDHLLAKTNAAFLDCRTLADQERFLLDGEPVVVYRTPGHSED
                     SICFHAGNDLITGDTLFNATVGNCFTGDLRGFFESIRRLMALPDNTRIFAGHDYVRDS
                     LAFARLLEPDNPEIERFRQICDPYFLHSTLAQEKKMNPYLRFNEAPIVRLLQERNLPR
                     ATEWERWQSLMTIE"
     gene            complement(738..1277)
                     /locus_tag="PLA58_09950"
     CDS             complement(738..1277)
                     /locus_tag="PLA58_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOR63156.1"
                     /translation="MLSRTAAVLILIDFQGKLAQSMCDKEDLFANTVRLIRGFRALGL
                     PLLVTEQTPEKLGTTIPEVALELGDAKPIAKETFSCWANPLFHDQLEALTRRHVVLTG
                     IESHVCVYQTALDLMQNGYTVHLVTDAVSSRTPENRKVGIQAVKSAGGELSSTEMVLF
                     ELLKSAADPKAREIFKIVK"
     gene            1384..2163
                     /gene="fdhD"
                     /locus_tag="PLA58_09955"
     CDS             1384..2163
                     /gene="fdhD"
                     /locus_tag="PLA58_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOR63157.1"
                     /translation="MTLPANEKVLLHEYRESGFVEVSLPFSTEYAVTLSINGRPFVTM
                     ACSGSYLKEHMAGYLITEGIISDPDQIEKIDIDEARLTVKVFLAEDRIIAQKLECIKT
                     ITAAGGRTKKNWPSEDLIRKRLPQVRANTILQSMSEFLSYSREHEATHGVHSAALYSL
                     EGERLVFFDEIGRHNAIDKVIGHAALHRISLEDKMICSTGRISSEIAFKIINARAPVL
                     ISRASPTTLSVELLRRFNILSVIRAVSGRFFVVNGKENIRL"
     gene            complement(2219..3820)
                     /locus_tag="PLA58_09960"
     CDS             complement(2219..3820)
                     /locus_tag="PLA58_09960"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOR63158.1"
                     /translation="MNIGSLADDNIRRFGEYDFVCFDGRWHTNVAMNQGANRLGNALK
                     KLGVKKGDRVGVQLFNCTELIQTFFAAFKIGAILVPINPSLKTPELAYIYRDAGLSAL
                     VSSPDDFGGIREARKEAPGLAHVISTGPQAPEDGLSFNGICAAQPDALALADTDNDDT
                     AVMIYTAGTTGNPKGVMLTHYNWYTHVTGYYDRVFLDSWGVSVRGETREDGGQTGKDP
                     RESEVFGVSRDRVSLITLPLFHGYGIFALNLEFLTGGKLVVINRWDPEEAMKRIETYR
                     VTDFRGVPTMYIQILNHPSLAKYDLRSLRTCICGAAPMPLAVARAWKEKFGIDIWEGY
                     GLSEATTVNCGNIAERRPPKYGSIGKCYQKCNAIKIFDSQDRELPAGQTGEIVIRGPG
                     VMKGYWNKPVETAAAIRNGWLHTGDVGRMDDDGYLYITDRKKDLIIRGGENIYPKEIE
                     NILYRHPDVLEAGVIGVPDPVYGESVKAFVALKTPGSVTEEELLEFCKKELPSYKRPK
                     AVQFLESLPKSAVGKILRRELRNIK"
     gene            complement(3887..>4300)
                     /gene="mce"
                     /locus_tag="PLA58_09965"
     CDS             complement(3887..>4300)
                     /gene="mce"
                     /locus_tag="PLA58_09965"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOR63159.1"
                     /translation="KEIESMKILKIDHIGIAVKNLAESSRLYEILGIPRAGVEEVAEQ
                     KVKVSFFPVGDSEIELLESTEPSGPIAKYIEKNGEGIQHMALRVDDLEAALAELKAKG
                     IRLIDEKPRYGAGGAKIAFVHPKSTGGILLELCER"
BASE COUNT          828 a         1212 c         1271 g          989 t
ORIGIN      
        1 cttcagcctt cagccttcgg cctccctcct actcgatcgt catcagcgac tgccagcgct
       61 cccattcggt ggcgcgcgga agattgcgct cctgaagcag tcggacaatg ggggcttcgt
      121 tgaatctcag gtagggattc attttctttt cctgggccag cgtggagtgg aggaaatacg
      181 gatcgcagat ctgccggaag cgttcgattt cgggattgtc cggttccaga agccgtgcaa
      241 aagccagaga atccctcaca tagtcgtgcc cggcaaagat gcgcgtattg tccgggagtg
      301 ccatcagtcg ccggattgat tcaaaaaatc cgcgcaggtc cccggtaaag caattgccga
      361 ccgtcgcgtt gaacagcgtg tcgccggtga taagatcgtt ccccgcatga aagcagattg
      421 aatcctcgga gtgtccgggt gtccggtaaa cgacgaccgg ctcgccgtcc agaagaaacc
      481 tttcctgatc ggccagcgtc cggcagtcca gaaacgccgc atttgttttt gccagaaggt
      541 ggtcgtcacc cggcgtgtgg tcgtagtgcc ggtgcgtatt ggcgacatat cggagcgaca
      601 gcgtgtgccg ctccagaaac gacaggattt cctgccaggc gccgccgtcg atggccatcg
      661 cttcggtctt gccgtaaaga agatacgcca gattgtcccc gtaccggaat tgttcaatgt
      721 ggatcatgcc gtttccctta tttcacgatt ttaaaaattt ctctggcttt cggatcggcg
      781 gcgctcttca gcagctcgaa caaaaccatt tccgtggatg aaagctctcc gccggcgctt
      841 ttgacggcct gaatgccgac cttgcggttt tcgggcgttc gggaagagac ggcgtcggtg
      901 accagatgaa ccgtatagcc gttctgcatc aggtccagcg ccgtctggta aacgcagacg
      961 tggctttcaa tgccggtcag gacgacatgg cgacgggtga gggcttccag ttgatcgtgg
     1021 aaaaggggat tggcccagca gctgaacgtt tccttggcaa tgggtttggc gtctcccagc
     1081 tccagggcca cctccggtat cgtcgttccc agtttttccg gggtctgctc cgtgaccaga
     1141 agaggcaggc cgagcgcccg gaatccccga atcaaccgga cggtgttggc aaaaagatct
     1201 tccttgtcgc acatggactg ggccagtttg ccctgaaaat cgatcaggat gagcacggcg
     1261 gcagttcggc tgagcatggt tcctcccttt gattttgctg atgcggcagg atagttaatt
     1321 tgcttaattc ttgtcaatca ttattattgc tggtaaaagg acaaaaagtc tggagacaag
     1381 aaaatgacgt tgccggcgaa tgaaaaggtt cttttgcatg agtatcgcga aagcggcttt
     1441 gtggaagtgt ctctcccgtt ttccacggag tatgccgtga cgctgagcat caacggccgc
     1501 ccctttgtta cgatggcctg ctccggaagt tatctgaagg agcacatggc cggctatctg
     1561 atcaccgagg ggatcatcag cgatccggat caaattgaga aaatagacat tgacgaggcc
     1621 cgtctgacgg tcaaggtctt tttggcggaa gacaggatca tcgcccaaaa actcgaatgc
     1681 atcaaaacca ttaccgcggc gggggggcga accaaaaaaa actggccgtc ggaggatttg
     1741 atccggaaga ggctgccgca ggtgagggcg aacacgattc tgcaatccat gagcgagttt
     1801 ctgtcttatt cgcgggagca tgaggccacg cacggcgtcc acagcgcggc gctctattcg
     1861 ctggagggtg agagactggt cttcttcgac gagatcggcc gccataacgc catcgataaa
     1921 gtcatcggcc acgcggccct ccatcgcatc agcctggaag acaagatgat ttgttcgacg
     1981 ggccgcatct cgagcgagat cgccttcaaa atcatcaacg cccgcgcgcc ggttctgatt
     2041 tcgcgcgcct cgccgaccac gctgagcgtc gagcttttgc gccggttcaa tatcctgagc
     2101 gtcatccgcg ccgtcagcgg ccggttcttt gtcgtgaacg ggaaagaaaa catccggctg
     2161 tagggcccga tcggatggcg gttcgttttg tacgccgttt cttgggttgg cggtgtcgct
     2221 acttgatgtt ccgcagttcg cggcgcagaa tttttcccac cgcgcttttg ggcagggact
     2281 ccagaaactg aacggctttg ggccttttat acgaaggcag ctcctttttg cagaattcca
     2341 gcagctcctc ctcagtgacg gaaccgggcg ttttcagggc gacgaacgcc ttgaccgatt
     2401 cgccgtacac cgggtcggga acgccgatga cgcccgcttc aagcacgtcg ggatgccggt
     2461 acaggatatt ttcgatctcc ttgggataga tgttttctcc gccccggatg atcaggtctt
     2521 tcttccggtc ggtgatgtac agatagccgt cgtcgtccat gcggcccaca tctcccgtgt
     2581 gcagccagcc gttgcggatc gcggccgccg tttccacggg cttgttccag tagcccttca
     2641 tgacgcccgg ccccctgatg acgatttctc ccgtctggcc ggcgggcagt tcgcggtcct
     2701 ggctgtcgaa gattttaatg gcgttgcatt tctggtagca cttgccgatg gagccgtact
     2761 tgggcggccg ccgttccgcg atattgccgc agttgacggt cgtcgcctcg ctcaggccgt
     2821 agccctccca gatgtcgatg ccgaattttt ccttccaggc gcgcgccacg gcaagcggca
     2881 tcggcgccgc gccgcagatg caggtccgaa gcgaacgcaa atcgtacttg gccagcgatg
     2941 gatggttgag gatctggatg tacatggtcg gcacgccgcg gaaatccgtg accctgtatg
     3001 tttcgatgcg cttcatggct tcctcggggt cccagcggtt gatgacgacc agcttgccgc
     3061 cggtgaggaa ttcaaggttc agggcgaaga tgccgtagcc gtggaacagc gggagggtaa
     3121 tcagcgaaac ccggtcgcgg ctgacgccga agacttccga ttcccgcgga tcttttccgg
     3181 tctgtccgcc gtcctctctt gtctcgccgc ggaccgacac gccccaggag tccagaaaca
     3241 cccggtcgta gtatcccgtg acgtgggtgt accagttgta gtgggtcagc atcacgccct
     3301 tggggtttcc cgtggtgccg gcggtgtaga tcatgacggc cgtgtcgtca ttgtccgtgt
     3361 ccgcgagagc aagcgcatcc ggttgtgccg cgcaaatgcc gttgaaagaa aggccgtctt
     3421 cgggtgcctg aggtccggtg gaaatcacat gcgcaagccc gggcgcttct ttgcgggctt
     3481 cccggatgcc gccgaaatcg tcgggcgaac tgaccagggc cgaaaggccc gcgtcgcggt
     3541 aaatgtaggc cagctcgggt gtcttcagcg acggattgat cggaacgagg atggctccga
     3601 ttttgaaagc ggcaaagaag gtctggatca gctcggtgca gttgaaaagc tgcacgccga
     3661 cccggtcgcc ttttttgacg cccagcttct tcagggcgtt gcccaggcgg ttggccccct
     3721 ggttcatggc gacgttcgtg tgccaccggc cgtcaaagca gacaaaatca tactcgccga
     3781 atctgcggat gttgtcgtcg gccagacttc cgatattcat ggttcccctc ctcaggaatg
     3841 cgctctttgc gcaagatgaa taaaaggcgg tccgctcgtc ggggttttat ctttcgcaga
     3901 gctccagcag aatcccgccc gtggatttgg ggtgaacgaa ggcgattttt gcgccgcccg
     3961 cgccgtagcg gggcttctca tcgatcaggc ggatgccttt ggccttcagt tcggccagcg
     4021 ccgcttccag gtcatccacg cgcagggcca tgtgctgaat gccctcgccg tttttttcaa
     4081 tgtatttggc aatggggccg gaaggctccg tggattccag aagttcgatt tccgaatccc
     4141 ctacggggaa aaaagaaact ttcacctttt gctcggccac ttcttccacg cccgcccggg
     4201 gaatgccgag tatctcgtag agcctggagc tttccgccag attttttacc gcgatgccga
     4261 tgtgatcgat tttcaaaatt ttcatacttt caatctcctt
//
LOCUS       DAOSRM010000136         4058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_19907_length_4058_cov_111.957282, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000136 DAOSRM010000000
VERSION     DAOSRM010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19907_length_4058_cov_111.957282"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLA58_09970"
     CDS             complement(<1..236)
                     /locus_tag="PLA58_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63160.1"
                     /translation="MKNRIDNPNIQCITVGMIKRTSFIEQLSTALRRSPVTALLGPRQ
                     CGKTTLAQTFGQNRVSVYEISLQNTLRLAAGMNA"
     gene            complement(521..1912)
                     /locus_tag="PLA58_09975"
     CDS             complement(521..1912)
                     /locus_tag="PLA58_09975"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR63161.1"
                     /translation="MRKGENQNKEYKQAWNDNCLKTICAFANTGGGKLFIGIDDEGNP
                     MGVKDAARYLDDIPNKTKDILGITPDVTVKKKAGKDIIEVTVAPSSAPISYHGRFYTR
                     SGSSSVEIKGHALVDLLMRKSGRSWDGFIEEGATLKDIDTSAINKFRKLAAKKIPQIA
                     AEKNVQAILQKLNLLEKGKLRRAAVLLFGKNPKKFYITAYIKIGKFVSATDLISTDDI
                     EGNLFDQVDKAIEMLRVKYLLSNITYEGIYRKDNMEYPEEALREAIINAVIHRNYMGA
                     HTQLRIDPDSLNLWNEGGLPPGIAVADLKKWHLSRPRNELLADVFFKAGMIEAWGRGT
                     VKIVDECKKAGLPEPEFREEFGGLSVHFRKADKAQEAQVEAQEAQVELLNWQREVMKY
                     CFDDEKTGNEIMKIAGYSSRTGNFKKGLQRLLDYQLLELTIPDKPQSRLQKYRLTDKG
                     RRLIAGMRGDKRK"
     gene            complement(1931..2104)
                     /locus_tag="PLA58_09980"
     CDS             complement(1931..2104)
                     /locus_tag="PLA58_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63162.1"
                     /translation="MKTCSNCGQPIQENDMENAAEILGGLQEASTGEVDDQNLCQACK
                     EELGMLSLMGFGE"
     gene            complement(2273..2776)
                     /locus_tag="PLA58_09985"
     CDS             complement(2273..2776)
                     /locus_tag="PLA58_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63163.1"
                     /translation="MRTIVCDAGPIIHLYEAHSLQLLRRTGDLFLPSRVFDEVHAVLD
                     LNDPWPEWLSVETLSLHEQNEAGIWKKSGDLHDGEAEALVLARRKKADWFLTDDSTTR
                     LFVALLGMEVHGSLGVILWNAAHGHLSRKETELALDNLEASSLWLSVRIYQEARQSLD
                     AIFDANP"
     gene            complement(2773..2976)
                     /locus_tag="PLA58_09990"
     CDS             complement(2773..2976)
                     /locus_tag="PLA58_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63164.1"
                     /translation="MKTLSVTVPDHLAECINDYVKAGFFLSEPDVVLAAMSEFVRRNR
                     MDLMERFAREDIEWAIKEAHAAK"
     gene            complement(3077..>4058)
                     /locus_tag="PLA58_09995"
     CDS             complement(3077..>4058)
                     /locus_tag="PLA58_09995"
                     /inference="COORDINATES: protein motif:HMM:NF022379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOR63165.1"
                     /translation="LWAKNWSDDIKIVRKEYTADNYRKTGEKCIADYYNKYAPFKERV
                     LGIETEEYVNLDPEGEFKWNVRMDRLDYAGDGKYEIHDYKTAGYLPKQEQADEDRQLA
                     LYSLWVKENFRDAENVELVWHYLAFDREVRSERTAEQLENLREETLQAVKKVVSAKEF
                     PANESNLCNWCEYKSICPKWAHLFKVETLPPKEFKKEDGVRLVDALAVLSEQKKELDT
                     QIEAVKDDLIAYARNLGVEVVFGSNKKATVSIRKDIAFPAKNTEEREALIQLLKAEGL
                     WGEVEDLDIFALKKIVQSREWDEDVLNKLVKYQEEKTSATVRLGKLNKEE"
BASE COUNT          834 a         1089 c          982 g         1153 t
ORIGIN      
        1 catgcattca tccctgcggc aagccgcagg gtattctgca aagagatttc ataaaccgaa
       61 acacgatttt gaccaaatgt ctgcgccagc gtggttttgc cgcattgacg gggacccagc
      121 agggcagtca ccggtgaccg tcgcaaggca gtggatagct gttctatgaa ggatgttcgt
      181 ttaatcatgc caaccgttat acattgaata ttcggattgt cgattcgatt tttcaatggt
      241 ttatattaaa tccaaagtga ccgctctttt cagaagtcct gcaaacgagg cagggctgaa
      301 catgcgtgcg agaaacaatg cttttttctt cacttcacaa cctcccagta cccgcctttg
      361 gccgggccga cgcgttttat ccggccggct tcacggagct ttcttaccgc ccgctccccc
      421 gcacttgctg atttgcctac ttgttcggcc aattccggta tggtcaggct gggattactc
      481 tggaggcgct ccataatttt ccccggcgtt ctcaccctgc ttatttcctt ttatctcccc
      541 tcatcccggc tataagccgc cgacccttgt cggtgagacg gtatttttgc agacggcttt
      601 ggggtttgtc gggaatagtc aattcaagaa gctgataatc aagcagcctt tgcaggcctt
      661 tcttgaaatt ccctgtgcgg ctgctataac cggcaatttt catgatttca ttgcctgtct
      721 tttcatcatc aaagcagtat ttcatgactt cccgctgcca attgagaagt tcgacttggg
      781 cttcttgggc ctcgacttgg gcctcttggg ccttgtccgc tttgcgaaaa tggacggaca
      841 agccgccgaa ttcttcccgg aattccggtt ccggcaatcc tgcctttttg cattcgtcca
      901 caatcttgac ggtgccgcgg ccccacgcct caatcatgcc cgctttgaaa aagacgtcgg
      961 ccagcagctc attgcggggg cgggataagt gccatttctt taaatccgca actgcgatgc
     1021 cgggcggcag gccgccttca ttccagagat tgaggctgtc cggatcgatc cgcagttggg
     1081 tgtgcgcgcc catgtaattg cggtgaatga cggcgttgat gatggcttcg cgcagagcct
     1141 cttccggata ttccatgttg tccttgcggt aaatgccttc atacgtgatg ttggaaagca
     1201 gatatttgac ccgcaacatt tcaatcgcct tgtccacctg atcaaagagg ttgccctcaa
     1261 tatcgtccgt gctgatcaga tcggtggcgc tgacaaattt gccgatcttg atataggcgg
     1321 tgatgtaaaa tttcttcggg ttttttccaa aaagcaaaac ggcggcacga cgcagtttgc
     1381 ctttttccag aaggtttaat ttctggagga tcgcctgcac gtttttttcc gccgcaattt
     1441 gcgggatttt tttggcggcc agtttccgaa atttgttaat ggcggatgtg tcaatgtctt
     1501 ttagggttgc gccctcttcg ataaatccgt cccaggagcg tccggatttc cgcatcagca
     1561 gatccaccag cgcatgcccc ttgatttcaa ccgaggaact gccgcttcgt gtataaaaac
     1621 ggccgtggta ggaaatcggg gcggaagacg gggcgacggt tacttcaata atatccttgc
     1681 cggctttctt tttgacggtt acatccggcg taatgcccag aatgtctttg gttttattgg
     1741 ggatgtcgtc aagatagcga gctgcgtctt tcacacccat cgggttgcct tcatcgtcaa
     1801 tgccgataaa gagctttccg ccgccggtgt tggcaaaagc gcaaatggtt ttcaaacagt
     1861 tatcattcca ggcctgtttg tattctttgt tttgattttc acccttacgc ataataatgg
     1921 tcctcagagt tcactcgcca aaccccatca ggctgagcat gcccagctct tccttgcagg
     1981 cctgacaaag attctgatcg tcaacctcgc cggttgacgc ctcttgaaga ccgcctaaaa
     2041 tctccgccgc attttccatg tcgttttcct ggatgggttg tccgcagttg ctgcatgtct
     2101 tcatacacct atccttttga ttattcaagg gttcaactaa atctgaataa tgcctttggc
     2161 ctcgtccgaa aagaagtcaa ggagcgggta gaggcctgtg ggcgtgatct gtgatgctgg
     2221 aactatgtgt gtcgcgttct tcctgcatct tgattctgag tgcttgtttg cgtcagggat
     2281 ttgcatcaaa aatggcgtcg agggattgcc gcgcctcctg gtaaatgcgg acggaaagcc
     2341 agagcgacga agcttcaaga ttgtccagcg ccagttcggt ttcctttcgc gacagatgcc
     2401 catgggcggc attccaaagg atcactccaa gcgacccgtg gacttccatg cccaggagag
     2461 cgacaaaaag ccgcgtcgtt gaatcgtctg tgagaaacca gtcagccttt ttcctgcgcg
     2521 ccaggacgag ggcctccgcc tcgccgtcat ggaggtctcc ggatttcttc cagatcccgg
     2581 cttcattttg ttcatggaga gaaagggtct cgacgctgag ccattcaggc cagggatcgt
     2641 tcagatcgag cacggcgtga acttcgtcaa acacacgcga gggcaggaac aggtcgcctg
     2701 ttcgtctgag caactgcagg gaatgagcct cataaagatg tatgatgggt ccggcatcac
     2761 agacgatggt tctcatttgg ctgcatgagc ctctttaata gcccactcga tgtcttctcg
     2821 ggcaaaacgc tccatcagat ccatccgatt acgtcggacg aattccgaca tggctgccag
     2881 gacgacatcc ggctcggaca ggaaaaatcc cgccttcaca taatcgttaa tgcactcggc
     2941 cagatggtcc ggcacggtga ccgatagtgt cttcatgggc gaccgtctcc tttctgaaaa
     3001 ttaccttgat tttcttatca tttttctctg ttatccgcaa gcgccgccaa gctatgattt
     3061 ctgtatgtat ggttttttat tcctccttgt tcaacttccc cagtctcact gtggcgcttg
     3121 tcttctcttc ctgatacttc acaagcttgt tgagaacatc ctcgtcccat tccctgctct
     3181 gaacgatttt cttcagagcg aaaatatcca gatcctcgac ttcaccccac agaccttcag
     3241 ccttaagcaa ttgaatcagg gcttcacgct cttctgtgtt cttcgcagga aaggcgatat
     3301 ctttccggat ggaaacagtc gcctttttat tggacccgaa aacaacctcg acgccaaggt
     3361 tccgggcata agcgatcaga tcatccttga cggcttcgat ttgtgtatcc agttctttct
     3421 tttgctctga aagaacggca agcgcgtcga caagcctgac gccgtcttcc tttttgaact
     3481 ctttcggcgg cagggtttcc actttgaaca ggtgcgccca tttcggacag atgcttttat
     3541 attcgcacca gttgcagaga tttgattcat tggccgggaa ctctttcgcg ctgacaacct
     3601 tctttaccgc ttgaagcgtt tcttcgcgca ggttttccag ttgctccgcg gttctttcag
     3661 agcgcacttc tctgtcgaag gccaggtaat gccagaccaa ttcaacgttt tcggcatccc
     3721 tgaagttctc tttcacccaa agggaataaa gcgccagttg ccggtcttca tccgcctgct
     3781 cctgtttggg aagatatccc gcggttttgt aatcgtggat ctcgtatttc ccatcgcctg
     3841 cgtaatccag cctgtccatc cggacgttcc atttgaattc cccttcagga tcgaggttga
     3901 cgtattcttc cgtctcgatg cccagcacgc gttctttgaa aggtgcatac ttgttgtaat
     3961 agtcggcaat gcacttctcg ccggttttgc ggtagttgtc cgccgtgtat tctttccgga
     4021 cgattttaat atcgtccgac cagttttttg cccacagg
//
LOCUS       DAOSRM010000137         3906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_20934_length_3906_cov_95.391846, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000137 DAOSRM010000000
VERSION     DAOSRM010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20934_length_3906_cov_95.391846"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..>1180)
                     /gene="hemW"
                     /locus_tag="PLA58_10000"
     CDS             complement(73..>1180)
                     /gene="hemW"
                     /locus_tag="PLA58_10000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOR63166.1"
                     /translation="FKGFDTIYIGGGTPSLLSIEQIENLLAAVHRHFQISPAHANVEH
                     HSLNVDKPATNHIPRHTGEYPDHLKVARGRKRRYPEDVEITMEVNPGDVSKTYLQDLK
                     ALGVNRLNIGVQSFDDRLLYFLGRRHSAKEALAAIDDARKAGFQNIGLDLIYGVADQD
                     TRLWIKTLEKALSFAPEHLSCYQLSLDEKTSLYRQYREKGAQPPSENEALDFFMTTSR
                     ILTDAGYIHYEVSNFARAENLRSRHNMKYWRHVPYLGLGPAAHSFCDRRRWWNTADVD
                     AYIRGLSEGKTPVGSSEELSTEQLAMEALFLGLRTKDGIDLGQYQARYGVDLLEQKTQ
                     TLHQLAEEGLVEIKDGCLRPTLSGMAVADSLALI"
     assembly_gap    1181..1280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1635..3431)
                     /gene="lepA"
                     /locus_tag="PLA58_10005"
     CDS             complement(1635..3431)
                     /gene="lepA"
                     /locus_tag="PLA58_10005"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOR63167.1"
                     /translation="MENIRNFSIIAHIDHGKSTLADRLIQHTGVCNERNFQDQLLDNM
                     DIERERGITIKSNAISLPYRAKDGKDYILNLIDTPGHVDFSYEVSRSLASCEGVLLLI
                     DAAQGVQAQTLANLYLAMEHNLEIIPIINKIDLPSADIDRVLEEIDSELGLDPDTHIK
                     ASAKEGTGIEEILEAIVERIPPPTGGEENPLAALIFDAKYDAYRGTIIHCRVFDGSVK
                     AGDIIRFMYNNATYKVEEVGHFLLTRARREKLSAGEVGYIIAGVKTVSDVRTGDTITH
                     QDRPCLQPLPGFKEVKPVVFASIYPIASDDYQDLADSLEKYKLNDASFIYEKDSSAAL
                     GQGFRCGFLGLLHLDIVQERLEREYDLSIILSVPSVRYRFTLKDGKVIYVDNPAHYPD
                     PAEIDKGEEPYIRASMMLPERYLGSVMKLCMEKRGVNSNMNYTGPGRVELSYEMPLAE
                     VIFDFYDRFKSVTQGYGSFDYDIIDYRESNLVLMDILVNGEKVDALSQIVHRERARAR
                     ALIACDRLKDEIPRQMYKIAIQGAIGSEIIARSTISAFRKDVTAKCYGGDITRKRKLL
                     EKQKEGKKRMKMIGSVSIPQSAFLAVLKSDSD"
     gene            complement(3499..>3906)
                     /locus_tag="PLA58_10010"
     CDS             complement(3499..>3906)
                     /locus_tag="PLA58_10010"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417462.1"
                     /note="essential enzyme that recycles AMP in active cells;
                     converts ATP and AMP to two molecules of ADP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOR63168.1"
                     /translation="LAQAEALWAALQKENIKLDIVIEIDLDRETAKKRIMGRRLCKMD
                     NNHPNNIYIDAIKPAEKNGKIVCRVCGCEELATRADDQDAAAIDKRHGIYYDTKTGTL
                     AAVNYFKERTKVIVVDGRAGVKEVSEDLMKKLG"
BASE COUNT          777 a          965 c         1132 g          932 t
ORIGIN      
        1 tcatgatgga ctgatataac gccacgcgtt aaacgtcaga cggaaaaatt gccgggtcaa
       61 aagggggctg ttttaaatga gcgccaggct gtccgcgacg gccatgcccg aaagcgtcgg
      121 acgcaggcag ccgtccttga tttccacaag gccctcctcc gccagttgat ggagggtttg
      181 tgttttctgt tcgagcagat ccacgccata tcgggcttga tattggccga ggtcaatgcc
      241 gtctttcgtg cgcaggccga gaaaaagcgc ttccatggcc aattgctccg ttgaaagctc
      301 ttcggatgac ccaaccggcg ttttgccttc ggagaggccc cggatatagg catcgacgtc
      361 cgccgtattc caccagcgcc tgcggtcgca aaacgaatgg gcggcgggtc ccaaccccag
      421 ataggggaca tgccgccagt atttcatgtt gtgtctggac cggaggtttt ccgcgcgcgc
      481 gaagttggac acttcgtaat gaatataacc cgcatccgtc agaatccgcg atgttgtcat
      541 aaagaaatcc agcgcctcgt tttcagatgg cggttgcgcg cctttctccc ggtattgccg
      601 gtagagagat gttttctcgt cgagcgagag ttggtagcag gacagatgtt ccggggcaaa
      661 ggacagggct ttctccaggg ttttgatcca gaggcgggta tcctggtcgg caacgccgta
      721 gatcaaatca aggccgatgt tttgaaatcc ggcttttcgg gcgtcatcaa ttgcggccag
      781 agcttctttt gcggaatgtc tgcgtccgag aaaatacaaa aggcggtcgt caaaggattg
      841 cacgccgata ttcaggcggt tgacgcccag agctttcagg tcctgaagat aggtcttaga
      901 aacgtctccc ggattgactt ccatggtgat ttcaacgtct tccggatacc ggcgcttgcg
      961 ccctcgcgcc acctttaggt ggtcagggta ttcgccggta tgacggggga tgtgattggt
     1021 tgccggttta tcaacgttca aagaatgatg ttcaacattg gcatgggcag ggctgatttg
     1081 aaaatggcga tggacggcgg caagtagatt ttcgatttgt tcaatggaaa gaagggaagg
     1141 cgtcccgccg ccgatataaa tcgtgtcaaa gcctttgaaa nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn gatttgcaga aagccatttc ctgtacgagg gccttcacaa
     1321 attcaggaat ccgatcaggg caggcaacgg agtaaaagct gcaatagccg catttgcttt
     1381 tacagaaagg gatgtgaaga taaagaccgg cttgatccat atgcattgat ttaggtcctt
     1441 ggtgcacaaa atatccattt gaacggacat ggggagcgtt ttggttgata aatcttggcg
     1501 aagcgcagga taagcgctga caactgtttg agccgaaggc gagttttgtc agcgcctgaa
     1561 gcgagccgta gatttaccca aaacgctcac tgtccgtgaa aaggattttt gtgtgacata
     1621 tgaccagcgt gtcgttagtc gctatccgac ttcagcaccg cgagaaacgc gctctgcggg
     1681 atgctgaccg acccgatcat cttcatgcgc ttcttgcctt ccttctgctt ctccagcagt
     1741 tttcgtttgc gcgtgatgtc gccgccgtag cacttggcgg tgacgtcctt gcggaacgcg
     1801 ctgatcgtgc tgcgggcgat gatttccgat ccgatggcgc cctgaatggc gatcttgtac
     1861 atctggcgcg ggatttcgtc cttcaggcgg tcgcaggcga tcagcgcccg ggcgcgggcg
     1921 cgctcccggt ggacgatctg ggaaagcgca tcgacctttt ctccgttgac cagaatgtcc
     1981 atcaggacca ggttgctttc ccggtaatcg atgatgtcgt agtcgaacga gccgtagccc
     2041 tgcgtgacgg atttgaaccg gtcgtaaaag tcgaaaatga cctcggccag cggcatttcg
     2101 taagagagtt ccacgcggcc ggggccggta taattcatat tggaattgac gccgcgtttt
     2161 tccatgcaca gcttcatgac gcttcccaga tagcgctccg gcagcatcat ggaagcgcgg
     2221 atataaggct cctcgccttt atcaatttcc gcggggtcag gataatgggc tggattgtcg
     2281 acgtagatca ctttgccgtc tttgagggtg aagcggtaac gcacgctggg aacggaaaga
     2341 ataatcgaca ggtcatattc cctctccagt ctctcctgaa cgatgtccag gtgcagaagt
     2401 ccgaggaagc cgcagcggaa gccctgcccc aatgcggcgg aggagtcttt ttcgtagatg
     2461 aacgatgcgt cgttcagctt atatttttcg agcgagtccg ccagatcctg gtaatcgtcg
     2521 gacgcgatgg gatagatcga cgcgaagacc acgggcttga cttccttgaa tcccggcaga
     2581 ggctgcaggc agggcctgtc ctggtgtgtg atggtgtcgc ccgtgcgcac gtcggagacg
     2641 gtcttgacgc cggcaatgat gtagcctacc tcgcctgcgg aaagtttttc ccgtctggcg
     2701 cgagtgagga ggaaatgacc gacttcttcc accttgtagg ttgcgttgtt gtacatgaag
     2761 cggatgatgt cgccggcttt gacggagccg tcaaaaacgc ggcagtgaat gatggttccc
     2821 cggtaggcgt cgtatttggc gtcaaagatc agagccgcga gcggattttc ttcgccgcct
     2881 gtgggcggcg gaatgcgctc gacaatcgct tccaggattt cttcgatgcc ggtgccttcc
     2941 ttggcggaag ccttgatgtg cgtgtccgga tccagcccca gttcggaatc gatttcctcc
     3001 aagacgcggt cgatgtcggc cgacggaagg tcgattttat tgatgatggg aatgatctcc
     3061 aggttgtgct ccatcgcgag gtagaggttc gccagcgtct gggcctggac gccctgcgcc
     3121 gcgtcgatca gaagcagcac gccttcgcag gaggccagcg accgtgagac ttcgtaggaa
     3181 aaatcaacat ggcccggcgt atcgatcagg ttcaggatat agtccttgcc gtctttggcg
     3241 cggtagggca gggagatggc attgctcttg atggtgatgc cgcgttcgcg ctcgatgtcc
     3301 atgttgtcca gaagctgatc ctgaaaattt cgctcattgc agacgccggt gtgctggatc
     3361 agccggtcgg ccagcgtgga tttgccgtga tcgatatggg cgataatgct gaaatttctg
     3421 atattttcca taggggttta aaaaaagccc tccaaggagt ctggggggct ttttatccta
     3481 aaagcggtcc ggatgcaatt atccgagttt tttcatcagg tcttcggaaa cttccttgac
     3541 gccggcgcgg ccgtcaacga cgatcacttt ggttcgttcc ttgaagtaat tgacggcggc
     3601 aagcgttccg gttttcgtgt cgtagtaaat gccgtggcgc ttgtcgatgg cggccgcgtc
     3661 ctgatcatcg gcgcgggtgg caagctcttc gcagccgcaa acgcggcaga cgatcttgcc
     3721 gtttttctcc gccggtttga tggcgtcgat gtagatgttg ttcggatggt tgttgtccat
     3781 cttgcaaagc ctccggccca tgatgcgctt cttggcggtc tcgcggtcga gatcgatttc
     3841 aatcacgata tccagtttga tgttttcctt ctgcagggcg gcccagagcg cttccgcctg
     3901 cgccag
//
LOCUS       DAOSRM010000138         3839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_21377_length_3839_cov_103.979123, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000138 DAOSRM010000000
VERSION     DAOSRM010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21377_length_3839_cov_103.979123"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="PLA58_10015"
     CDS             complement(<1..1508)
                     /locus_tag="PLA58_10015"
                     /inference="COORDINATES: protein motif:HMM:NF006182.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-CoA synthetase"
                     /protein_id="HOR63169.1"
                     /translation="MSNICYRDTIGDMARRAATKYGDKIAIIFRDMKISFYDLERQAR
                     RFANLMTSYGVRKGDRVAIYAYNSHYYPISMMGLAKIGAIQVPINYMLNAEEIAYVVN
                     HSGSRIFIVEDILYPIIAKDQAKFTTVEKWGFLPLGDSLVPEGFFNLVTEMEAMTYDE
                     PQVEVSAEDIVQIPYTSGTESKPKGAMLSHRALMSQYHSCIFDGQYETDDVSLHALPL
                     FHCAQLHCFLMPYLYVGATNVIMHKADPLEMIRLIEKYQVTHMFAPPTVWIGILNHPE
                     FRNHYHSSLKKAAYGASIMPVEIIKQLAVTFRGLKLWNYYGQTEMGPVATILKPEDQL
                     LKPGSAGKPVLNVETRLMDDDGKFVPLGEVGEIVHRSGHVMTGYLNDPEKTAEAFEFG
                     WFHSGDLGRFDTDGYLYVVDRKKDMIKTGGENVASREVEEVLYQVPGVEEVAVIGLAD
                     PKWIEIVAAVVVPKKGAALGEKEIIAHCRERLAGFKCPKKVILVDRLPKNPSG"
     assembly_gap    1686..1785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1843..3486)
                     /locus_tag="PLA58_10020"
     CDS             complement(1843..3486)
                     /locus_tag="PLA58_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR63170.1"
                     /translation="MSGFVNKTMGDVIAETAGRFPDHPALIAPQFQVRLNYRELYEQC
                     RKTARGLMAMGVRRGDHVSVWTTNVPEWVYLQFALGMVGGILVTINTNYQSHELDYIL
                     RQSDSTTLFLIDRYRDTSFYETVRQIIPELDSCKPGKTASSKLPVLKNIVYIGNREST
                     PGMFKFSDLIAMGESVSEQDLDDRMKTLTDDDVINMQYTSGTTGFPKGVMLTHHNIIN
                     NARMVGDVMGMTDKDTLLIQVPVFHCFGCVMSTLNSVYHASTMVLLESFDPLLSLELI
                     HKEKCTAVNGVPTMFIAMLHHPDFERYDMTSLRTGIMAGAPCPVEAMNQVRTKMHCSE
                     VVIAFGQTESSPVMTMTRRDDPVDLRVATVGRLLPDIEGKIVDPESGEDLPADTQGEI
                     VTRSACVMKGYYKMPEATTAAIDSDGWLHTGDLGEIDPNGYFKVTGRIKDMIIRGGEN
                     IYPRELEEFIFTHPKVQNVQVVGIPDPKYGEQVLAAIHLKNGNQATAEEFIEFCKGKI
                     ARHKIPKYWEFVDEYPMTASGKIQKFKIKEMFEKKYKEG"
BASE COUNT          742 a         1045 c         1020 g          932 t
ORIGIN      
        1 cccgatggat tcttgggcag tctgtcaaca aggatcacct ttttgggaca tttgaacccg
       61 gccagcctct cccggcagtg ggcgatgatt tccttttcgc cgagcgccgc gccctttttc
      121 ggcacgacca ccgccgccac aatttctatc catttgggat cggccagccc gatgacggcc
      181 acttcctcaa cgcccggaac ctgatagagc acttcctcga cctcgcggga cgccacgttt
      241 tccccgccgg ttttgatcat gtccttcttg cggtccacca catagagata gccgtcggtg
      301 tcaaaacggc ccagatcgcc cgagtggaac cagccgaact cgaaggcctc ggcggtcttt
      361 tccggatcgt tgaggtagcc cgtcatgacg tggcccgagc gatggacgat ttccccgact
      421 tctcccaggg gaacgaattt tccgtcgtcg tccatcagcc gcgtttccac gttcagcacc
      481 ggcttgcccg ccgatcccgg cttcaggagc tgatcttccg gcttcaggat cgtggccacc
      541 ggccccatct ccgtctggcc gtaatagttc cacagtttga ggccgcggaa ggtcaccgcc
      601 agttgtttga tgatttccac cggcatgatg ctggcgccgt aggccgcctt tttcagactg
      661 ctgtggtaat ggtttctgaa ttccggatgg ttcaggattc cgatccacac cgtgggcggc
      721 gcgaacatat gcgtgacctg gtatttttca atgagccgga tcatctccag ggggtcggcc
      781 ttgtgcatga tcacgttggt ggcgccgaca tacaggtacg gcatgagaaa gcagtgcagc
      841 tgggcgcagt gaaacagggg cagcgcgtga aggctcacgt cgtcggtttc gtactggccg
      901 tcgaaaatgc agctgtggta ctgggacatc agcgcccggt gcgacagcat cgcgcccttg
      961 ggtttggatt ccgtgccgct ggtgtaggga atctgcacaa tgtcttcggc gctgacctcc
     1021 acctggggtt cgtcgtaggt catggcctcc atttccgtca ccaggttgaa gaagccttcc
     1081 ggcaccagcg aatcccccag cgggaggaag ccccattttt cgaccgtcgt gaatttcgcc
     1141 tggtccttgg cgatgatcgg atacagaatg tcttccacaa tgaagatcct ggatccggag
     1201 tgattgacga cgtaagcgat ctcttcggcg ttgagcatgt aattgatggg aacctggatg
     1261 gcgccgatct tggccagacc catcatgctg atggggtagt aatgggaatt gtacgcatag
     1321 atcgccaccc ggtcgccctt cctgacgccg tagctggtca tgagattggc aaagcgccgg
     1381 gcctgccgct caagatcata aaacgaaatt ttcatgtcgc gaaaaatgat ggcgatcttg
     1441 tcgccgtatt tggtggccgc cctgcgggcc atgtccccga tcgtatcccg atagcaaatg
     1501 ttgctcatgt ttatatcctt tcgcttttgt tcttgggctt tccctattgc ttgcaggaac
     1561 ggttataaac cgttccctac acctggggaa gataggtttt taggctgaag gctgaaggtc
     1621 atgtggaagc cgtcgattct tttttatctg aagatcccgc cttcagtctt ttgcctttgt
     1681 cttttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccttt gtcttttacc
     1801 ttcagccttc ggccttcagt cttcagcctt tatcttttgc cttcagcctt ctttatactt
     1861 cttctcaaac atttccttaa tcttgaactt ctgaatcttg ccgctggccg tcatcggata
     1921 ttcgtccacg aactcccagt atttcgggat cttgtgacgc gcgattttcc ccttgcagaa
     1981 ttcgatgaat tcctccgccg tggcttgatt cccgtttttc agatggatgg ccgcgaggac
     2041 ctgctcgccg tatttgggat ccggaatgcc gacgacctga acattttgca ccttgggatg
     2101 cgtgaagata aattcttcca gctcccgggg ataaatattt tcgccgcccc ggataatcat
     2161 atccttgatc cggccggtaa ctttaaaata gccgtttggg tcgatctcgc ccaggtcccc
     2221 cgtgtgcagc caaccgtcgg aatcaatggc ggcggtcgtg gcttccggca tcttgtagta
     2281 gcctttcatc acgcaggcgc tgcgcgtgac gatttcgccc tgggtatccg cgggcaaatc
     2341 ttcgccgctt tctggatcga cgatttttcc ttcgatgtcc ggcagcagac ggccgaccgt
     2401 ggccacgcgc aggtccaccg gatcatcgcg gcgggtcatg gtcatgaccg gcgaggattc
     2461 cgtctggccg aacgcgatga cgacttcgga acagtgcatt ttcgtgcgca cctgattcat
     2521 ggcctctacc gggcagggcg ctccggccat aatgccggtc cgcagggacg tcatgtcgta
     2581 gcgttcgaaa tcgggatgat ggagcatggc gatgaacatg gtcggcacgc cgttgacggc
     2641 cgtgcatttc tctttgtgaa tcaactccag agacagaagc gggtcgaaag actccaaaag
     2701 caccatggtg gaggcgtggt aaacgctgtt gagcgtgctc atgacgcacc cgaagcagtg
     2761 aaagacgggc acttggatga gcagcgtgtc cttgtccgtc atccccatga catcgccgac
     2821 catgcgggcg ttgttgatga tattgtgatg ggtcagcatc acgcctttcg gaaaccccgt
     2881 cgttcccgac gtgtactgca tattgatgac atcgtcatcg gtgagcgttt tcatccggtc
     2941 gtccagatcc tgctccgaaa cgctctcgcc catggcgatc agatcggaaa atttgaacat
     3001 gcccggcgtg ctttcccggt tcccgatata gacaatgttt ttcagcaccg gtagtttgga
     3061 agaagccgtc ttgccgggtt tgcaggaatc cagttcggga atgatctgcc ggaccgtttc
     3121 gtaaaaactg gtgtcccggt accggtcgat cagaaacagc gtggttgaat ccgattgtct
     3181 gaggatgtaa tccagctcgt gagactggta attggtattg atggtcacca gaatgccgcc
     3241 caccatcccc agggcgaatt gcagatacac ccattccggc acgttggtcg tccagacgga
     3301 aacgtgatcg ccgcgccgga cgcccatcgc catcagaccc cgggcggttt tgcggcattg
     3361 ttcataaagt tcgcggtagt tgagccggac ctgaaactgc ggagcaatca aagccggatg
     3421 atcgggaaac cggccggccg tttctgcaat cacatccccc atcgttttgt tcacaaaacc
     3481 tgacataacc cacctcatcc tgtaatattt ttcagcgtca cgcggaacca cgccgggctc
     3541 ccgcttagcg tcactgctgc gcaggcgtcc tttgttggat cacttaacgt aaactggggt
     3601 gaatatacgt gatttataga aaatgtcaat aaaatcattg aatattggcg ctattctttt
     3661 ttcaggccgg gccgggtgcg gggacacgga gcaagtattt gataatattc gattccggca
     3721 ctcgcgtccc cgcgcatcct ttagccggtg aaacattcag gggcttcgcg gaaaaaacaa
     3781 ccggatggtg aaatacgggt ggacaacgcg gaaaagaatg agagctttga atgacggcc
//
LOCUS       DAOSRM010000139         3803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_21644_length_3803_cov_114.418090, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000139 DAOSRM010000000
VERSION     DAOSRM010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21644_length_3803_cov_114.418090"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..686
                     /locus_tag="PLA58_10025"
     CDS             126..686
                     /locus_tag="PLA58_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOR63171.1"
                     /translation="MNKEFIAIRWHGRGGQGAITAAKIVAGAAFGSGYAGVVMAPTFG
                     TERRGAPVMTSLKISKTKIYDLSPIETPDIVVVLDHLLLKEADVTHGLKPGGIIVLNT
                     PMAHESYHFQDYRLATADITAISVEAGLPHGMVNTGIIGSLEKAANLLGIDLLVSGIE
                     AEFRTRKPEKNSLAAKIAYERTVAGG"
     gene            689..997
                     /locus_tag="PLA58_10030"
     CDS             689..997
                     /locus_tag="PLA58_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR63172.1"
                     /translation="MGTRNYRRKTSHSEHGAGDGGRTGSWRVERPVLDRNLCIPCKRN
                     KEACFICWLFCPDVVISRTIPPTINLEYCKGCGICAEECPTKAITMVDEAKFAEEDKE
                     "
     gene            1003..2202
                     /gene="porA"
                     /locus_tag="PLA58_10035"
     CDS             1003..2202
                     /gene="porA"
                     /locus_tag="PLA58_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOR63173.1"
                     /translation="MHIIENGNAAAALGVKLCRASVIAAYPITPQTPLTEKLSEYVEL
                     GEMKAEYIPVESEHSALAVCIAASSAGARTFTATSANGLLYMHEQVQWASGARLPMVM
                     CVVNRAVGAPWNVWNDQQDSISQRDTGWIQIYCADHQQIIDTVIKAYWLAEQVSIPVM
                     VCYDGYILSHTFMPFDLPDQEQVDSFLPPFQPDYFLNPQDPANLNTVTLPDVRMDVRG
                     ELAHGYMEIRCLLQEDLRKALQTAAEAEKRFAGIFNRGADPYIEPYRCDDAEHVAVGL
                     GSITYQMRVCVDQLRTEGIKIGVMGVRYYRPFPDEAIAAALKGKKGVIVVEKALSYGY
                     EGALMSDLKSALYEHLAGTGSLPVVQNFIAGIGGREIRTDDLTQTLRAAAKGRVSKEP
                     VWIGLRL"
     gene            2212..3114
                     /locus_tag="PLA58_10040"
     CDS             2212..3114
                     /locus_tag="PLA58_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOR63174.1"
                     /translation="MQAKSTILSLPTEELVHPGTRACTGCGLSIAYRVGLKALGRDTI
                     LVVPPSCLTVLQGLFPVASTKLPCLNVTFASTAAAAAGILATLKAQGNEHTTVAGWAG
                     DGGTSDIGLQALSGAAERGDNFIYFCYDNEGYMNTGVQRSGTTPIGAITANTPFKGKL
                     QQKKDVPAIMAAHHLSYVAACSAAYPLDLYDKIKNCLALPGTKYFHIHIPCPPGWGYD
                     PRYGIKIGRLAVETGYYDLYEIVNGEFKLTAASEKLIEKRKLVPVREYFRAQSRFRTL
                     TEAQIGDIQKTIDEKWAGYYKKDE"
     gene            complement(3191..>3803)
                     /locus_tag="PLA58_10045"
     CDS             complement(3191..>3803)
                     /locus_tag="PLA58_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOR63175.1"
                     /translation="MEKDKVIDYDWKIWKGGLIKGEKLERFINAAVNHTPIEKLKIPF
                     YAVATNAGTGEEMVFGRGNTGVAVHASCSVPGVFQPVRIGKDTYVDGGVVSPVAVDVA
                     RRYGADVVIAVDISGGLDKDAPSGVMNTMQKTFNIMYAKNVAAQLKNADVVIRPNVEE
                     IGSTDMEKFNQAIFEGEKAATAALPEIRKVLDKLNAEGRLPVR"
BASE COUNT          820 a         1150 c         1095 g          738 t
ORIGIN      
        1 agtattgaac caaaagttac tgaaactgaa aaagtgctga acggcttgat caaatctttg
       61 caaagagatt gatttttcac cttcagtctt cagtcttcag cctaaccccc tcaggatgtt
      121 ttaacatgaa caaagaattt attgccatcc gctggcacgg acgcggcggc cagggcgcga
      181 tcacggcggc caagattgtg gccggcgccg cattcggttc cggctatgcg ggcgtggtca
      241 tggcgccgac cttcggcacc gagcgaagag gagcgccggt catgacgtcg ctcaagatct
      301 cgaagacgaa aatttacgat ctgtcgccca tcgaaacgcc ggacatcgtc gtcgtcctgg
      361 accatctgct cctcaaggag gcggatgtca cccacggcct caagcccggc ggcatcatcg
      421 tcctgaacac gccgatggcc cacgaatcct atcattttca ggattaccgg ctggccaccg
      481 cggacatcac ggccatatcg gtggaagcgg ggctgccgca cggcatggtc aacaccggca
      541 ttatcggctc cctggaaaaa gccgcgaatc tcctgggaat cgaccttttg gtttcaggca
      601 ttgaagccga atttcgcacg cgaaaaccgg aaaagaactc cctggcggct aaaatcgcct
      661 acgagagaac cgtggcggga ggctgaacat gggaaccaga aattatcgaa gaaaaacgtc
      721 gcacagcgaa cacggggcgg gagacggagg aaggacggga tcctggaggg tggagcggcc
      781 ggttctcgac cggaacctct gcattccctg caaaagaaac aaggaggcct gcttcatctg
      841 ctggctgttc tgtccggacg tcgtgatttc gcggacgatt ccgcccacca tcaatctgga
      901 atactgcaaa ggctgcggca tctgcgccga agaatgcccc accaaggcca tcaccatggt
      961 ggacgaagcc aaattcgcgg aagaagacaa ggagtagagg ccatgcatat catcgaaaac
     1021 ggaaacgccg cagcggctct gggcgtcaag ctctgccggg ccagtgtaat cgcggcctat
     1081 cccatcacgc ctcagacacc gctcacggaa aagctctccg aatacgtcga attaggcgag
     1141 atgaaggcgg aatatatccc cgtggaaagc gagcactccg cgctggccgt ctgcattgcc
     1201 gcgtcctccg ccggcgcccg gacttttacg gccaccagcg ccaacggact gctgtacatg
     1261 cacgagcagg tgcagtgggc atcgggcgcg aggctgccga tggtcatgtg cgtggtcaac
     1321 cgggccgtcg gcgccccctg gaatgtctgg aacgaccagc aggattccat ctcgcagcgg
     1381 gacacgggct ggatacagat ctactgcgcg gatcaccagc agattatcga cacggtcatc
     1441 aaggcttact ggctggccga gcaggtgagc attcccgtga tggtctgcta cgacggatat
     1501 attttatccc acaccttcat gccgtttgat ctccccgatc aggaacaggt ggattctttt
     1561 ctgccgccgt tccagccgga ttattttctc aatccccagg accccgccaa tctcaatacg
     1621 gtgacgctgc cggatgtgcg gatggatgtc aggggcgaac tggcccacgg atacatggaa
     1681 atccgctgcc tgctgcagga agacctgcgc aaagcgctcc aaaccgcggc cgaggcggaa
     1741 aagcgctttg ccggaatctt caaccgcggc gccgatccct acatcgaacc gtaccgctgc
     1801 gacgacgcgg agcatgttgc cgtcggcctg ggatcgatca cctaccagat gcgcgtttgc
     1861 gtggaccagt tgagaacgga aggcatcaaa atcggcgtga tgggcgtccg ctattaccgc
     1921 ccgtttccgg atgaggcgat tgctgcggcc ctgaaaggga aaaaaggcgt gatcgtcgtc
     1981 gaaaaggcgc tgagctacgg atatgaaggc gccctgatgt cggatttaaa gtcggcgctg
     2041 tatgaacacc tggccggaac cggatcgctt ccggtcgtcc agaatttcat cgcgggcatc
     2101 ggcggccgcg aaattcgaac cgatgatttg acgcaaaccc ttcgcgccgc ggcgaaaggc
     2161 cgggtttcca aggagcccgt ctggatcggg cttcggctat aggagaatgc catgcaggcg
     2221 aaatcaacca ttttaagttt accgacggaa gaactggtcc atccgggaac cagggcgtgc
     2281 acgggctgcg gcctgtccat cgcctaccgg gtggggctga aggccctggg ccgtgacacc
     2341 attctggtgg tgccgcccag ttgcctcacc gtgctgcagg ggcttttccc ggtggcctcg
     2401 acgaaactgc cctgtctgaa cgtgacgttt gcttcaacgg ccgccgccgc agcgggcatc
     2461 ctggccacgc tcaaagccca gggcaacgag cacaccacgg tcgccggctg ggcgggcgac
     2521 gggggaacca gcgacatcgg cctgcaggcc ctgtccggcg cggcggagcg cggcgacaac
     2581 ttcatctatt tttgctacga taatgaaggc tacatgaaca cgggcgtgca gcgctcggga
     2641 acgacgccca tcggcgccat caccgcaaac acaccgttca aggggaagct tcagcagaaa
     2701 aaggatgtgc cggccatcat ggccgcgcac catctaagct acgttgcggc ctgctcggcg
     2761 gcctacccgc tggacctcta cgacaaaatc aaaaactgcc tcgcgctgcc cggcaccaag
     2821 tatttccaca ttcacattcc ctgccctccg ggctggggct atgatccgcg ctacggaatc
     2881 aaaatcgggc ggctggccgt ggaaacgggc tattacgatc tttatgaaat cgtcaacggt
     2941 gaattcaagc tgacggccgc ttcggaaaag ctgattgaaa agcgaaagct ggtcccggtc
     3001 cgcgaatact tccgcgccca gtcccgcttc cggacgctga ccgaggcgca gatcggcgac
     3061 atccagaaaa ccattgatga gaaatgggcg ggatactaca agaaagacga gtgacgatca
     3121 tcggacgcag gctccgggat ccgcgcttct ggggcgcttt tcccggtaaa aactgacggc
     3181 cgccattccc ctatcggacc ggcaaacggc cttcggcgtt cagtttgtcc aacactttcc
     3241 gtatttcagg cagcgccgcc gtggccgcct tttccccctc gaagatcgcc tggttgaatt
     3301 tctccatgtc ggtcgatccg atttcctcca cgttcggacg gatgacgacg tcggcgtttt
     3361 tcagctgggc ggcgacgttt ttggcgtaca tgatgttgaa tgttttctgc atggtattca
     3421 tcactccgct gggggcgtcc ttgtccaaac cgccggaaat atccacggcg atcaccacat
     3481 ccgcgccata ccgtctggcc acatccacgg ccaccggact gaccacgccg ccgtccacat
     3541 acgtgtcctt cccgatcctg accggctgaa acacccccgg cacgctgcaa ctggcgtgca
     3601 cggcgacgcc ggtgttgccc ctgccgaaca ccatttcctc accggtgccg gcgttggtcg
     3661 ccacggcgta gaacgggatt ttgagcttct cgatgggggt atggtttaca gcggcattga
     3721 tgaaacgctc gagtttttcc cctttgataa gtccgccctt ccagatcttc cagtcgtagt
     3781 cgatcacctt gtccttttcc atc
//
LOCUS       DAOSRM010000140         3765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_21924_length_3765_cov_108.715633, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000140 DAOSRM010000000
VERSION     DAOSRM010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21924_length_3765_cov_108.715633"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..385)
                     /locus_tag="PLA58_10050"
     CDS             complement(110..385)
                     /locus_tag="PLA58_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63176.1"
                     /translation="MKKTLGTIVAAAAVVLLTATFGFAEYAAAGAAQFPYFQLGCLIV
                     GGLIMVSLKRKYNKMYTAEVVGAFALYTLLMALFTNPVIDAVKNIVT"
     gene            778..1407
                     /locus_tag="PLA58_10055"
     CDS             778..1407
                     /locus_tag="PLA58_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63177.1"
                     /translation="MNLNHPEATQQALNMLRSGDPFQWYVIPLLVMVLYIYANEYQKG
                     NYRVIAGGLALYMVHWFVEIVNALVQYFSGHALWTVPAGTAFEILVGVGIEISLMFSI
                     AGIVVCKLMPKDPAVRILGIPNRLFFAVVNAAFASILEIFLVRTPAFVWVYGWWGAFP
                     VFVTVYIPFFAAACYVHDWQPRSQKLFIGSLFLANASALIVFAGILGWI"
     gene            1425..1907
                     /locus_tag="PLA58_10060"
     CDS             1425..1907
                     /locus_tag="PLA58_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63178.1"
                     /translation="MKWFLKCVTAGVILAFVFGCASSGTMLKSETVGAIPDGKYETII
                     RLDTPECLPLQPDAGFIYVEPEGKVITDFPRQVAVKNLDSLDRYERLNRDRMGAFVIK
                     DRRGRVRGYYQLLFEYRAFIWERGEDILLQITIPRGRRGAIADAGINAAGMGAISVGR
                     "
     gene            complement(1933..3381)
                     /locus_tag="PLA58_10065"
     CDS             complement(1933..3381)
                     /locus_tag="PLA58_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR63179.1"
                     /translation="MKQKVHRLDPFFRPQSIALFGSMQEGWFFGAAVIISDLMKWNYT
                     GKIYPVHPTARTVYGIPVFQDLAQVKDVPELAVIVTSFRHAPGILEQCGAKGIRAAVI
                     VSDGFGEAGPEGKIRQEELLRIAKAHGIRVMGPNTVGVFNSESRFTTVPYDRGYEYAK
                     QGKLSIITQTGMYSPQAMAWHEYEAGINKVIDLGNMSDVDETDCLEYLSRDDSTDVIS
                     LYMEHSRRPRVFLDALKKASLRKPVLCLLPGGSPGAAQAMASHTGSMAGNAELYQAAL
                     RQGGALRVEEYEDLRDCATPFLRYPLPQGNRLGIITFSGAIGIQAIDAAEACGLSLGK
                     LSSESRRELAAVSGTLGGHPIDVGPASATAGAGIFDLYQKCFDILAADENIDCIYLNT
                     YVFNGLKPAYYEDLLGRIGSCRGKPVVSWCYGPSQQLVCEFGKLAERHGIPFYLNSRK
                     AVRSLSYMARYAHWKAVASGKPLRRPSVHPAG"
BASE COUNT          798 a          967 c         1081 g          919 t
ORIGIN      
        1 taatgtccgt attgctgcat cctgtgtgtc ttgatcatgg ccctgtcctc ctttccaaat
       61 gtctctttct tctagatgtg tcttctatcg accctgctgc tgctgttgat caggtcacaa
      121 tgtttttcac cgcatcaatc accggattgg tgaagagcgc catcagaagg gtgtaaagag
      181 cgaaggcgcc gacgacttcc gccgtgtaca tcttgttata cttcctcttc aggctgacca
      241 taatcagtcc gcccacaatc aggcagccca gctgaaaata cgggaattgg gccgcccctg
      301 ccgccgcata ctctgcaaat ccaaaggttg ccgtcaaaag aaccactgcc gccgccgcta
      361 cgattgttcc taaggtcttc ttcatgatct gtttcctcct tcaagggttt ttttgtttct
      421 ttcgctttat ttctaatgaa atttatgtgc caaaaatgga aaatcgatag agaatcgcgg
      481 caagtatagg atttacaatg caaataaact gaaggtcttc cggtgaacag agctcccaaa
      541 agtccttttc cccgcttttt cttaatcaaa tgttcaacga ggttgaacgt tatgatgaaa
      601 aaatctggtg tgaaaaaaaa gagtcgggta taagtagtgc gaaccttcag gcccgctttt
      661 tgagcgcagc taaagctacg cactacgttg gtggggttat catctggtgt agtgcgggtc
      721 ttcagacccg ctgaaaacaa aagaggggaa cctcttgttt ttgaaaggag gccggcgatg
      781 aatttgaacc accccgaggc aactcagcag gcgctgaata tgttgcgcag cggcgaccct
      841 tttcagtggt atgttattcc tctcctggtg atggtcctgt acatctatgc caatgagtat
      901 caaaagggca attaccgcgt gattgccggc ggactggccc tttacatggt ccactggttt
      961 gtggaaatcg tcaacgccct cgtgcagtat ttctccggcc atgccctgtg gaccgttccc
     1021 gcgggaaccg cctttgaaat tctggtgggt gtcggaatcg aaatcagcct gatgttttcg
     1081 atcgccggaa tcgtggtctg caaactgatg ccgaaagacc ctgccgtcag gattctgggg
     1141 attccgaatc gtttgttctt tgccgtcgtc aacgcggcct ttgcctccat tttggaaata
     1201 ttcctggtgc ggacgccggc gtttgtctgg gtttatggct ggtggggcgc tttcccggtt
     1261 ttcgtgacgg tttacattcc tttctttgcc gccgcctgct acgtgcacga ctggcagccc
     1321 agaagccaga agctgtttat cggctctctt tttctcgcca atgcctccgc cctgattgtc
     1381 ttcgcgggca ttctcgggtg gatctgagaa agggaggaat gaaaatgaaa tggtttttga
     1441 aatgtgtgac ggcaggggtt attctggcgt tcgtcttcgg ctgtgcgtcc tccgggacga
     1501 tgctcaaaag tgagacggta ggcgccatac cggacgggaa atatgagacg attatccggc
     1561 tcgatacgcc ggaatgcctt ccgttgcagc cggatgccgg gtttatttat gttgaaccgg
     1621 agggaaaggt catcacggat ttcccgcgtc aggtcgccgt aaaaaacctc gattctctgg
     1681 atcgctacga acggctgaac cgggatcgca tgggggcgtt cgtcatcaaa gaccgccggg
     1741 gcagggtgcg ggggtactac caattgctgt tcgagtaccg ggcgttcatc tgggagcggg
     1801 gggaggatat tttattgcag atcacgattc ccagaggcag gaggggcgcc atcgcggatg
     1861 ccggaataaa cgccgcgggc atgggggcga tttccgtcgg ccggtaaacc gcgcggccgg
     1921 cggcagggct gttcatccgg cggggtggac ggacgggcgc cggagcggtt tcccggaagc
     1981 gacggccttc cagtgcgcat accgggccat gtaggaaaga gaccggacgg ctttacggct
     2041 gttgagataa aaaggaatgc cgtggcgttc ggccagtttt ccgaattcgc aaaccagctg
     2101 ctgcgacggt ccgtagcacc agctgacgac cggttttccc cggcaggacc cgatccggcc
     2161 aagcaagtct tcataatagg ccggtttcaa tccgttgaaa acataagtat tcaggtaaat
     2221 acaatcgatg ttttcatccg cggcgaggat atcgaagcat ttttggtaca ggtcgaatat
     2281 tcccgcgcct gccgtggcgg aagccggtcc cacgtcaatg gggtggccgc ccagcgtgcc
     2341 gcttacggcg gcaagctccc tccggctttc cgaagaaagc tttccgaggg aaaggccgca
     2401 ggcttcggcg gcatcgatcg cctggatgcc gatggccccg gagaaagtga taatgccgag
     2461 acggtttccc tggggcaggg gataacggag aaacggggtg gcgcagtcgc ggaggtcttc
     2521 gtattcctcc acccggagag cgccgccctg cctgagcgcc gcctggtaca gttcggcatt
     2581 gccggccatg gatcccgtgt gcgaggccat ggcttgagcc gcgccgggag aaccgcccgg
     2641 caggaggcac aaaacaggct tgcgaagaga agccttcttt aaagcgtcca gaaaaacccg
     2701 gggcctccgg gagtgctcca tgtagaggga gatcacgtcc gtgctgtcgt cccgactgag
     2761 atattccaga cagtccgttt cgtcgacatc gctcatgttg cccagatcaa tcactttgtt
     2821 gattccggct tcgtattcat gccacgccat ggcctgcggg ctgtacatgc cggtctgggt
     2881 gatgatggaa agttttccct gctttgcata ctcatatccc cggtcgtagg gaaccgtggt
     2941 gaaccggctt tcggagttga agaccccgac ggtattcggt cccatgaccc ggatcccgtg
     3001 ggcttttgcg atccgcaaga gctcttcctg acggattttg ccttccggcc ccgcttctcc
     3061 gaaaccgtcg gaaacgatga ccgcggcccg gatgcctttg gcgccgcact gttcgagaat
     3121 gcccggcgcg tgccggaagg aggtgacgat gacggccagt tcgggaacgt ccttcacctg
     3181 ggccagatcc tgaaagaccg ggattccgta aacggttcgg gcggtgggat gaacgggata
     3241 gatttttccc gtgtagttcc atttcatgag gtcgctgatg atgacggcgg cgccgaaaaa
     3301 ccagccttcc tgcatgctgc cgaacagggc gatgctttgc ggacggaaaa acggatccag
     3361 ccggtgaacc ttttgcttca tgccagtgct cctttatgga agtgacgtgt ggggctaccc
     3421 ttatcccaac ggaggcaaca tttcaaatgg taattgatca gagggcaggg tttgacgaga
     3481 gtccgggttt gcagtttcct gccggatcag tcgttgcggg gaaataaaaa aggttcatac
     3541 ggttcttgtg tgcttcctgg atgcgagcat ttgttaattt tcatgggcgt ccaaccttat
     3601 gcatacgaaa ttaatgacga tcactgctac ggcgacctga aatattcttg gaatggttat
     3661 aaagggactg ctgaaaatat ttcacaccac aaagaggaag ttctaataaa ttaagacggc
     3721 aaggcccgta ccgagtcaat tgcatatatt ttttctgttt ttgcg
//
LOCUS       DAOSRM010000141         3715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_22303_length_3715_cov_111.929508, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000141 DAOSRM010000000
VERSION     DAOSRM010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22303_length_3715_cov_111.929508"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1924
                     /locus_tag="PLA58_10070"
     CDS             116..1924
                     /locus_tag="PLA58_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOR63180.1"
                     /translation="MAEKFISDRNLRFLLYEVNDVESLLKYPRFADHSREVFDMILDT
                     AMRMGKDLFKPLFEEMDRKAPEYVGGEVKVHPKVREIMRAAGDGGWIASTFPYELGGQ
                     QMPFTVGVMLPTAIFGAANYSAAVYHGLTHGAAGLIASYGTQEMKDTYLPKMTAGEWQ
                     GTMALTEPQAGSSLTDLTTTATPTDQGYYKIKGQKLFISAGDHDGCDNVIHLALVRLK
                     DAPPGIKGISLFIIPKKRIRDDGSLEPNDVNCAGIYHKLGYRGSPITQLAFGENDDCR
                     GWLVGEANKGLAYMFQMMNGARIDVGLAAAVISSAAYYASLEYARERPQGRRLTSKDP
                     LSPQIPIIEHADIRRMLLFQKAVVEGSLSLLFQCGIYEDLEHVTEGEERENYNLLLEL
                     LTPVAKTYPSEMGILSCSAGLQILGGYGFCEEFPLEQLYRDVRIHPIHEGTTGIQGMD
                     LLGRKMTMKKGKAAQLYLAEVRKAIKEARDDAELKPFADKLAEAVALLEKVTMHQFGV
                     AAKEGPEMFLADATVYLEFFGIIAIAWQWLRQATVAQRALAGGPGEADTNFYQGKLAA
                     FRYFFGYELPKIQGLSDRLLNQDGLTVKMKNEYFED"
     gene            2053..2319
                     /gene="rpsT"
                     /locus_tag="PLA58_10075"
     CDS             2053..2319
                     /gene="rpsT"
                     /locus_tag="PLA58_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOR63181.1"
                     /translation="MATHKSAEKRDRQAKARRTRNIAAKSAIKTKVKAVIAAVESKNR
                     ENSEKALKAAIPAVAKAASKGLIHKKNASRKISRLARKVNAVKE"
     gene            complement(2401..3519)
                     /locus_tag="PLA58_10080"
     CDS             complement(2401..3519)
                     /locus_tag="PLA58_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR63182.1"
                     /translation="MILFLLTFLCLYGGINFYFFFRARSVLHFSGALQALLLVLLIFL
                     ILAPVVVRTLESLHCEQAARFVACTGYVWMAFVFLFFFLSVSLELVRFLHKLIVPEIG
                     TMALKAVTFGLSVLLSAGLVVYGCIDAQRVRVRTLQIPTEQILPGKGKIRIIQISDVH
                     VGIIIRNDRLSPMLEKVREAKPDMLVSTGDLLDGELDNIMADAERFAAIETPAGKFAV
                     LGNHEYYAGVSRSIEFTEAAGFQILRDDVTQAAGLTIFGEDDITGRKPDATPKSAAFL
                     KALAEKQNGFVLLLKHQPHIREKANFNLQLSGHTHGGQLFPFGFIVKLYFPRIYGLHT
                     LAENKLLYISRGTGTWGPPVRIFAPPEITVIDLIGKKQ"
BASE COUNT          914 a         1024 c         1054 g          723 t
ORIGIN      
        1 attacggggt aaacggaatc tccggagctg aaaggctccg gagatttttc acgaaatcgg
       61 gagaccctat ttttttggac ttccctatct ttgagtcaaa tagaaggaat ggattatggc
      121 ggaaaaattt atcagtgacc ggaacttacg attcctgctt tacgaagtca atgacgtgga
      181 gtcccttttg aaataccccc gctttgccga ccacagccgg gaagtattcg atatgattct
      241 ggatacggcg atgcggatgg ggaaagacct cttcaaaccc ctgttcgagg aaatggaccg
      301 caaggcgccc gaatatgtgg gcggcgaagt caaagttcat cccaaggtcc gcgaaatcat
      361 gcgcgcggcg ggcgacggcg gctggatcgc ctccaccttt ccctacgagc tgggcggcca
      421 gcagatgccc ttcaccgtcg gcgtgatgct gccgacggcg attttcggcg cggccaacta
      481 ttccgccgcc gtttatcacg gtctgacgca cggcgcggcc ggcctgattg catcctacgg
      541 cacgcaggaa atgaaagata cctacctgcc caaaatgacc gcgggcgaat ggcagggcac
      601 aatggcgctc accgaaccgc aggccggctc atcgctgacc gatcttacca ccacggcgac
      661 gccgaccgac cagggctact acaaaatcaa gggccagaag ctgttcatct ccgccggaga
      721 ccatgacgga tgcgacaatg tgattcacct ggcgctggtt cgtctcaagg acgcgcctcc
      781 cggcatcaag ggcatttcgc tcttcatcat tcccaagaag cgcatcaggg acgatgggtc
      841 actcgaaccc aacgacgtca actgcgcggg catttaccac aaactcggct accggggctc
      901 ccccatcacc cagcttgcct tcggcgaaaa cgacgactgc cgcggatggc tggtcggcga
      961 agccaacaag gggctggcct acatgttcca gatgatgaac ggcgcccgca tcgacgtggg
     1021 tctggccgcg gccgtgattt cctccgccgc ctactatgct tccctggaat acgccaggga
     1081 aagaccgcag ggacggaggc tgacctccaa ggatccgctg tcgccgcaga ttccgatcat
     1141 cgagcacgcg gatatccgtc gcatgctcct gttccagaaa gccgtcgtgg aagggtcgtt
     1201 gtcgctcttg tttcagtgcg gcatttatga ggacctggag catgtcaccg aaggcgagga
     1261 gcgtgaaaac tacaacctgc ttctggagct gctgacgccg gtcgccaaaa cctatccgtc
     1321 ggaaatgggc attttatcct gctccgcggg tctgcagatt ctgggcggct acggcttctg
     1381 cgaggagttt ccccttgaac agctttaccg cgacgtgcgc atccacccga ttcatgaggg
     1441 cacaaccggc attcagggaa tggacctgct cggccgaaag atgacgatga aaaaaggcaa
     1501 ggccgcccag ttgtatctgg cggaagtgcg caaggccatc aaggaggccc gggacgacgc
     1561 ggaattgaag ccgtttgccg acaaactggc cgaagccgtc gcgctcctgg aaaaagtaac
     1621 gatgcatcag ttcggcgtgg ccgccaagga aggcccggag atgtttctgg ccgacgccac
     1681 ggtctatctg gaattcttcg gcatcatcgc gattgcctgg cagtggctgc gccaagccac
     1741 cgtagctcaa agggcgctgg ccggcgggcc cggcgaagcc gacacgaatt tctatcaggg
     1801 aaagcttgcc gcgtttcgct acttcttcgg ctacgaactg cccaagattc aaggcttgtc
     1861 cgaccgcctg ctcaaccagg acggcctgac cgtgaagatg aaaaacgagt actttgaaga
     1921 ttgatcggct gtggatggag gcggtctcgc ggcctgcctc caatccgctt cccaaaattg
     1981 cgcttgacag cctgaaataa ttgcgttaaa gacccgcacc ttacaaaaaa agaagttcag
     2041 gaggagaacc ttttggcaac acacaaatca gcagaaaaac gcgatcgtca ggccaaggcc
     2101 cgccgcacgc gcaatatcgc agccaagtcc gcgatcaaga ccaaggttaa agcggtgatc
     2161 gcggctgtgg agagcaaaaa cagggaaaat tcggaaaaag cattgaaagc agcgattccc
     2221 gcggtggcga aagccgcttc caaaggcctg attcacaaga aaaacgcctc acgtaaaatt
     2281 tcccgattag ccagaaaagt caatgccgta aaggaataaa caatatataa atcagcaaca
     2341 tgagcgcggt cgtgctttcg ggcaggccgc gtttttattt cgccggcatt ccatttcccc
     2401 ttattgtttt ttgccgatca gatcgatgac ggtgatttcc ggcggggcga aaatgcgcac
     2461 gggcggcccc caggtgccgg tgccgcggct gatgtacagc agcttgtttt ccgccagagt
     2521 atgcaaaccg taaattctgg ggaaatagag ttttacgata aagccgaagg ggaaaagctg
     2581 gccgccgtgc gtgtggccgg aaagctgcaa gttgaaattg gccttttccc gaatatgcgg
     2641 ctggtgcttc aaaagcagaa caaagccgtt ttgcttttcg gccagcgctt tcagaaacgc
     2701 cgcgcttttc ggcgtggcgt cgggcttccg gccggtgata tcgtcttcac cgaaaatcgt
     2761 aagtcccgcc gcctgcgtca catcgtcgcg cagaatctga aagccggctg cttcggtaaa
     2821 ttcaatggag cgcgaaacgc ccgcgtaata ttcatgattg cccagaacgg caaatttccc
     2881 ggcaggcgtt tcgattgcgg caaagcgctc cgcgtccgcc atgatgttgt ccagttcgcc
     2941 gtcgagcagg tcgccggtgg aaaccagcat gtcgggtttg gcctcccgga ccttctccag
     3001 catgggcgaa aggcggtcgt tgcggataat gatgcccaca tggacgtcgg agatctggat
     3061 gatgcggatc tttccttttc cggggagaat ctgttctgta ggaatttgca gggtcctgac
     3121 gcggacgcgc tgcgcgtcga tgcagccgta aaccaccagt ccggcggaca gaagcacgga
     3181 caggccgaac gtcaccgcct tcagcgccat cgtgccgatt tcgggaacga tcagcttgtg
     3241 aaggaaccgg accagctcga gggaaacgct caaaaagaaa aacagaaaga caaacgccat
     3301 ccagacatag ccggtgcagg cgacaaagcg agcggcctgc tcacagtgaa gcgactccag
     3361 cgtccggaca acgaccggcg cgaggatcag gaaaatgagg aggaccagca gcagggcctg
     3421 cagggcgccg gagaaatgaa gaacgcttct ggcccggaaa aagaaataaa aattaattcc
     3481 gccgtaaaga cataaaaagg ttaaaagaaa tagaatcatc ctgatttcca tagtcggtta
     3541 ctatccgcct ggaaactaaa ggaaaacaag gcgaaagtca atcattaagg cagatcggtc
     3601 tttcggtcgc agattgaggt tgacaaggca aaacccttca tataaatacg acatctcttc
     3661 aaggcaggcc gatgtagctc agctggtaga gcaactgatt cgtaatcagt aggtc
//
LOCUS       DAOSRM010000142         3678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_22607_length_3678_cov_79.904223, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000142 DAOSRM010000000
VERSION     DAOSRM010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22607_length_3678_cov_79.904223"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..131)
                     /locus_tag="PLA58_10085"
                     /pseudo
     CDS             complement(1..131)
                     /locus_tag="PLA58_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(239..670)
                     /locus_tag="PLA58_10090"
     CDS             complement(239..670)
                     /locus_tag="PLA58_10090"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63183.1"
                     /translation="MAVKQISVLLGNVPGSFARMTHILHTEDINTIAVSAASIEEDSR
                     VRLVVNDPDRAAALLESFNFNVEVTPVLAAEIPLHAGHINAILKPLAKGDINILYLYT
                     TINRIGKETIVIIGVDKLEEAREILAENWINLIDDEIYSIP"
     gene            complement(680..1219)
                     /locus_tag="PLA58_10095"
     CDS             complement(680..1219)
                     /locus_tag="PLA58_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOR63184.1"
                     /translation="MITKTIFSGFGGQGVMMMGTSLAQSAMSKGFHVTYLPAYGAEMR
                     GGTANCTVAVSDDEIASPVASEPNYLVVMNQPALFTFQNRVTPGGSILINSSIVEASP
                     SRQDVTTFYIPCSDMAQELGNARTANIVMMGAFVRQSGIVPPEIYLKTLETIMGSRKK
                     ALADINRKAFTAGYEYVQK"
     gene            complement(1216..1989)
                     /locus_tag="PLA58_10100"
     CDS             complement(1216..1989)
                     /locus_tag="PLA58_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOR63185.1"
                     /translation="MAKIVFKHPESLKENIFHYCPGCGHSIVHRLVAEVIDEMGIRGI
                     TIGVPPAGCAVLAYNYFDVDMIEALHGRGPAMATAIKRCLPDRLVFSYQGDGDLAAIG
                     IAESFHAANRGENISVIFINNAVYGMTGGQMAPTTMLNQRSTTTPAGRKQAVDGYPVK
                     VSEIFSQLDGATYVERCSVSSPAGVNKTKKAIRKAFQCQMAGLGFSLVEVLSLCPTNW
                     KLSSVDSCKYIDEVMSKVFPPGVYKDQTGQSGKTAEARP"
     gene            complement(1997..3055)
                     /gene="vorB"
                     /locus_tag="PLA58_10105"
     CDS             complement(1997..3055)
                     /gene="vorB"
                     /locus_tag="PLA58_10105"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOR63186.1"
                     /translation="MNKILMSGNNVIGEAAIRAGCRFYAGYPITPQNELTEYMAEHMR
                     NAEGGVFIQSESEVAAINMIAGAAAAGVRVMTSSSGPGISLKQEGISSMAAQELPGII
                     VNIMRGGPGLGNILPSQGDYFQATRGGGHGDYRTIVFAPGSCQELADLTMDAFDLADK
                     YRTPAMILADGMMGQMMEPVIFKKPKPRQYPEKFMLRGAGSGKSKFIRGLLLDPVDSE
                     AHNWKLARKYEVISENETRCEEHRIDDAKLVIVAYGTAARIAKGAIKRLRSHDMKIGL
                     FRPITLWPFPEMRLRTLSEKVNNFLVFEMSTGQMLEDVRLALQGYADIEFHGRPGGAV
                     PTPAELANVIARVYEQKD"
     gene            complement(3048..3257)
                     /locus_tag="PLA58_10110"
     CDS             complement(3048..3257)
                     /locus_tag="PLA58_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR63187.1"
                     /translation="MKGYIFIKKELCKECGLCVHFCPKDHIIPSKEYNAQGYHPVCVD
                     ETKECNGCAICATMCPEIAIEVYRE"
BASE COUNT          711 a         1014 c         1067 g          886 t
ORIGIN      
        1 cgcgccagat ggcggttcag actctgggat ctttccctct ggcatttcac ggtaaggccg
       61 gtcggaaggt gggtcagctg aacgcaggtg gaagttttgt tgactttctg tcctccgggg
      121 ccggaggatc ggacaaagga ttcttcgatg tctcgttcgg aaacgccaag ctcctgcatg
      181 cgcagcgcaa gcgctttttc tttttccgcg gaaaccggca taagcggctc ctcacaactc
      241 aggggatgga gtagatttcg tcgtcgatca gattgatcca gttttcggcg agaatttcac
      301 gggcttcctc cagtttatcg acgccgatga tcacgatggt ttcttttccg atgcggttga
      361 tggtcgtgta caggtagaga atattgatgt cgcctttcgc cagcggtttc aggatggcgt
      421 tgatgtgccc cgcgtgcagg gggatttcgg cggccagcac cggcgtcact tccacgttga
      481 aattgaagct ctccaaaaga gcggcggcgc ggtcggggtc gttgaccacc agccggacgc
      541 ggctgtcctc ctcaatgctg gcggcggaga ccgcgatcgt gttgatgtct tccgtgtgca
      601 gaatatgcgt catcctggcg aaagaaccgg gaacattgcc cagcagaacg gaaatttgtt
      661 taacggccat acctgtcctt tacttttgaa cgtattcgta tccggcggta aacgccttgc
      721 ggttgatgtc cgccagcgcc tttttccggc ttcccatgat cgtctccagg gttttcagat
      781 aaatttcagg cgggacaatg ccggactgcc ggacgaaggc gcccatcatc acgatgttgg
      841 cggtccgggc gttgccgagt tcctgggcca tgtcggagca ggggatgtaa aaggttgtga
      901 cgtcctggcg gctgggggac gcctcgacaa tcgaggaatt gatcaggatg cttcccccgg
      961 gggttacgcg gttttgaaac gtaaacagcg cgggctggtt catcaccacc agatagttcg
     1021 gctccgacgc gacgggcgag gcgatttcat catcgctcac cgccacggtg cagttggccg
     1081 ttccgcccct catttccgcg ccgtaggcgg gcaggtaggt gacatgaaag cccttgctca
     1141 tcgcgctctg ggccaggctg gtgcccatca tcatgacgcc ctggcctccg aagcctgaaa
     1201 aaattgtttt ggtaatcatg gccgggcctc cgccgttttt ccggattgcc ccgtttgatc
     1261 tttatatacg ccgggaggaa agactttgct catgacctcg tcaatgtatt tgcaggaatc
     1321 gacggacgag agtttccagt tggtcgggca gagggaaagc acttccacca gagaaaaacc
     1381 caaaccggcc atctggcact gaaaggcttt ccgaatggct tttttggttt tgttgacgcc
     1441 cgccggcgag ctgaccgagc atcgctccac gtaggtggcg ccgtcgagct gggaaaagat
     1501 ttcgctgacc ttgaccggat aaccgtccac ggcctgtttg cggcccgcgg gcgtcgtggt
     1561 ggagcgctga ttcagcatcg tggtcggggc catctgtccg ccggtcatac cgtaaaccgc
     1621 attgttgatg aagatgacgc tgatattttc gccgcggttg gcggcatgaa aactttcggc
     1681 aatgccgatg gcggcaaggt ctccgtcgcc ctggtaggaa aaaacgagcc ggtcgggaag
     1741 acagcgcttg atggctgttg ccatggccgg accgcgtccg tgaagcgctt cgatcatgtc
     1801 cacgtcaaag taattgtagg ccagcacggc gcatccggcc ggcggaacgc cgatcgtgat
     1861 gccccgaatg cccatctcgt caatcacttc ggcgaccagc cggtggacga tgctgtgccc
     1921 gcagccggga cagtaatgaa aaatattttc tttcaggctc tcgggatgct tgaaaacgat
     1981 cttggccatg gttgaatcag tccttttgct catagactct ggcgatgaca ttggccagtt
     2041 ccgcgggcgt cggcacggcg ccgcccggtc gtccgtgaaa ttcgatgtcg gcgtatccct
     2101 gcagggccag tcgaacgtct tccagcatct ggccggtgct catttcaaac accaggaaat
     2161 tgttcacctt ttctgaaagg gtccgcagcc tcatttccgg gaagggccac agcgtgatcg
     2221 ggcggaaaag cccgattttc atgtcatggc tgcgcagcct tttgatggcg cccttggcga
     2281 tgcgcgccgc ggttccgtaa gcgacgatga cgagctttgc gtcgtcgatc cggtgttctt
     2341 cacagcgggt ttcgttttcg gagatcactt cgtatttccg ggccagtttc cagttgtgcg
     2401 cttcgctgtc caccggatcc agaagaaggc cgcggatgaa tttgcttttg ccgctgccgg
     2461 cgccgcgcag catgaatttt tcaggatact gccgtggttt gggctttttg aagattaccg
     2521 gctccatcat ctgccccatc attccgtcgg ccagaatcat ggcgggcgtg cggtatttgt
     2581 cggccagatc gaaggcgtcc atggtcaggt ccgccagttc ctggcagctt cccggcgcga
     2641 aaacgatggt gcggtaatcg ccgtggccgc cgccccgcgt ggcctggaag taatcgccct
     2701 gcgacggaag gatgttgccc agtcctggtc ctccgcgcat gatgttgacg atgattccgg
     2761 gcagctcctg cgccgccatg gaagaaatgc cttcctgctt cagggagatg ccgggtccgg
     2821 aggaggacgt catcacccgg acgcccgcgg ccgctgcgcc ggcaatcatg ttgatggccg
     2881 ccacttcgct ttcggactga atgaaaacgc cgccttcagc gttgcgcatg tgctcggcca
     2941 tgtattcggt cagttcgttc tgcggcgtga tcggatagcc ggcataaaaa cggcagcccg
     3001 cgcggatcgc ggcttcgccg atcacattgt ttccggacat cagaatttta ttcacggtaa
     3061 acctctatgg cgatttccgg gcacatggtc gcgcagatcg cgcagccgtt gcattctttg
     3121 gtttcatcga cgcacacggg atgatacccc tgcgcgttgt attctttgga cggtatgata
     3181 tggtctttgg ggcagaaatg cacgcagagt ccgcattcct tgcaaagttc ctttttgatg
     3241 aaaatatagc ctttcaaaac ctatccttaa atccgttttt gcccgggacg caattccttt
     3301 tggcggatgc tatcttgcgc ataatgttca taaagtcaag aaaatttagc tcatagcccg
     3361 cagcgcgtag ggtattgaag gaacggttct attttttaca atgtagtgcg gggtaaaccc
     3421 cgctgatacc gtgaccaata tcccgagagc tttgaataac ggccattttt gtcatttcga
     3481 ccgaagggag aaatcttgaa caaacggcca ttattagatt tctcgtcgct cacgctcctc
     3541 gaaatgacat ttttcaaagc tctcatcccg ctaaacccgg cgcggctaaa gccacgcact
     3601 gcaccgggtg gcacgaggtt gcgcctgtct catatctttc cgatctgaaa accttccagc
     3661 ttgatggcca ccgagcgc
//
LOCUS       DAOSRM010000143         3636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_22964_length_3636_cov_111.439263, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000143 DAOSRM010000000
VERSION     DAOSRM010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22964_length_3636_cov_111.439263"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..388)
                     /locus_tag="PLA58_10115"
     CDS             complement(209..388)
                     /locus_tag="PLA58_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR63188.1"
                     /translation="MAYVITDECIACGSCEDECPVQAISEGEDKYVIDAKLCTDCGAC
                     CDQCPVEAIVPGEEK"
     gene            complement(449..670)
                     /locus_tag="PLA58_10120"
     CDS             complement(449..670)
                     /locus_tag="PLA58_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOR63189.1"
                     /translation="MKEEVQKALEKVRPGLQADGGDVELIDVSPEGVVKVKLTGACHG
                     CPMSQMTLKMGIEKILKQQVPSVREVVSV"
     gene            complement(717..1307)
                     /locus_tag="PLA58_10125"
     CDS             complement(717..1307)
                     /locus_tag="PLA58_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63190.1"
                     /translation="MMKKVCARILLVVAVLSVIACGGLRYSQLDPAAKDYHPKRIAVF
                     PADVGTYEEARNAIEQIVPGVLMDKKWFTDVIDTATLYRQIQANDELRKISTEYISKL
                     QTLNYSDPILSKRIGELTHTDGFLLVAVDYWNYTVEKDKKLAKVSVGLKLIDPATGKI
                     MWKAGHHLGESYMLIKPDLPDVARNVVKDMVHEMPH"
     gene            complement(1347..3074)
                     /gene="ispH"
                     /locus_tag="PLA58_10130"
     CDS             complement(1347..3074)
                     /gene="ispH"
                     /locus_tag="PLA58_10130"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOR63191.1"
                     /translation="MEIKLAKTAGFCMGVRRAVDTVLDVSQHETGRKIYTYGPLIHNP
                     QTIELLKSRGITAIQSIDEIPDRQNALLIIRAHGIAPGERKKIKESGVKIIDATCPKV
                     GYVQAIIKKHTALDYTVIIAGDREHPEVDGLWGYTEGRGIVVSTLEDAEKIPALEKVC
                     IVAQTTQDSDHYREIVRKIQQKNPQAVVFNTICSSTEKRQEEIISLAAEMDALFVVGG
                     KNSANTRRLADLARKQNTPTFHIETVKELKNVDLGPYKSIGVSAGASTPNWIIDQITD
                     HLAEISSPTPKTAFLLKLWLWMVKTDFYSALGAGCLALAGMLLQNIPVAAASLAVASF
                     FVYAMHVLNRLVTSKESGLIGSFREPFYLRHEKIFRLSAFASLFIALTLSLAGSILAF
                     GLLLFISLAGGLYNMKLLPGRGRFERLRDIPGSKNFFTAFAWGIATAVLPALSAGCAF
                     SAGTAVAFIFTFILVFTRSALSDIMDMQSDRLLGRETIPVMIGKENTQILLKIILLIL
                     LVILILSPVAGWSPTPGLFLILCVLYVWICFSLCDRRAGFSGAIIEGLLETSYIIAGF
                     AVLGWLVFR"
     gene            complement(3064..>3636)
                     /locus_tag="PLA58_10135"
     CDS             complement(3064..>3636)
                     /locus_tag="PLA58_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOR63192.1"
                     /translation="YVSRGGLKLKGALDFFSLNVEGMAALDVGASTGGFTDCLLQAGA
                     RAVFAVDVAYGQLAWKLREDPRVTVIERTNIRHYNGAGLTEKPSLAVIDVSFISLATV
                     IPAVLPHLGDSARILALIKPQFEARREEVGKNGVVEDAAVQERVVREIASLCQSLGLR
                     VEGTCASPLLGPAGNKEFFILARKINGHGN"
BASE COUNT          788 a         1016 c          982 g          850 t
ORIGIN      
        1 atccttaaga tttctcgtcg ctcgcgctcc tcgaaatgac atttttcaaa gctctcgggg
       61 tcatgaaaaa cgacggcctt gtaaaatagc cggaaagcgc cttttcattt tgatggttgc
      121 cccgagggac ggacgatatt caaaaaaagc aggggaaaac ccgccggtcg ggacggacat
      181 tccccctacg taaaaagacg cgcagaactt atttttcttc tccgggaacg atcgcttcca
      241 ccgggcattg atcgcagcag gcgccgcagt ccgtgcacag cttcgcatca atcacatact
      301 tgtcttcgcc ttcggatatc gcttgcaccg gacactcatc ctcgcatgag ccgcaggcaa
      361 tgcattcatc tgtaatcaca taggccattg ttttagaaat ctcctttacg tcaaaaattt
      421 ttcgcgttta ccgtttttca gccgtcgttt agaccgacac gacttccctg acggaaggca
      481 cctgctgttt caggatcttt tcgatgccca ttttcaacgt catctgcgac atcgggcagc
      541 cgtggcaggc gccggtcagt ttgaccttga ccacgccttc cggagaaacg tcaatcaatt
      601 ccacatcgcc gccgtccgcc tgaagtcccg gccggacttt ttccagtgct ttttggactt
      661 cttccttcat aaatcatctc cctgtgtttt taagtgtttc ggattttgga tcgccgtcaa
      721 tgcggcattt catggaccat gtccttgacg acgttgcggg cgacatcggg caggtccggc
      781 ttgatcagca tgtagctttc ccccaggtga tggccggctt tccacatgat tttcccggtg
      841 gccggatcaa tcagcttcag gccgacgctg accttggcca gtttcttgtc tttctccacc
      901 gtgtaattcc agtagtccac ggcgaccagc aaaaagccgt ccgtatgggt cagttcgccg
      961 attcttttgc ttagaatggg atcggagtag ttcagggtct ggagtttgga gatgtattcc
     1021 gtcgatattt ttcgcagttc gtcattggcc tgaatctggc ggtacagggt ggccgtatcg
     1081 atgacgtccg taaaccattt tttgtccata agcacaccgg gcacgatctg ttcgatggca
     1141 ttgcgtgctt cttcataggt tcctacatcg gccgggaaca cggcaattcg tttgggatga
     1201 taatcctttg ccgccgggtc cagttgagaa taccgcagtc cgccgcaggc gatgacgctc
     1261 aaaacagcaa cgaccagaag aatccgtgcg cacacttttt tcatcatgat ccttaccccc
     1321 ttctttatgt tttgcaggca tcgactctac cgaaaaacaa gccagcccaa aacggcaaaa
     1381 ccggcaatga tatagctggt ttccagaagc ccttcaataa tggcccctga aaaccccgcc
     1441 cttctatcac aaagactaaa acaaatccat acatataata cgcataaaat caggaaaaga
     1501 ccgggtgtcg ggctccagcc ggcgaccggc gataatatta aaattaccag taatatcaag
     1561 aggataattt ttagaaggat ttgcgtattt tcttttccga tcatgacggg aatcgtctcc
     1621 cgccccagga gccggtcgct ttgcatgtcc atgatatccg acagggcaga ccgggtaaac
     1681 accaggataa atgtaaaaat aaaagcaacc gctgttccgg cgctgaaagc gcagccggcg
     1741 ctcaaagcgg gcagaaccgc cgtggcgatg ccccacgcga aggccgtaaa aaagtttttg
     1801 gaacccggga tgtccctcag ccgctcaaag cgccctctgc ccggcaacag tttcatgtta
     1861 tagagaccgc cggccagcga gatgaaaagc agcagcccaa acgccaagat gctgcccgcg
     1921 agcgacaatg tgagagcgat gaagagggaa gcaaaggcag acagacggaa tattttctcg
     1981 tgccgcagat agaatggctc gcggaacgag ccgatcagcc cggattcttt cgaagtgacc
     2041 aggcgattga gaacgtgcat ggcatagaca aaaaaggaag cgacggcgag agacgcagcg
     2101 gcgacgggga tattttgcag gagcatgccc gcaagcgcca gacacccggc gcccagggcg
     2161 gagtaaaagt ccgttttcac catccacagc cagagcttca ggagaaaggc cgttttgggg
     2221 gtgggagaac tgatttccgc caaatgatcc gtgatctggt cgatgatcca gttgggggtg
     2281 gaggcgccgg cggacacgcc gatgcttttg taggggccga gatcgacgtt cttcagttct
     2341 ttgacggttt cgatgtggaa cgtcggcgtg ttttgtttcc gggccaggtc ggccaggcgg
     2401 cgggtgttcg cgctgttttt gcctccgacg acaaatagag cgtccatttc cgcggccagc
     2461 gaaataattt cttcctgccg cttctccgtg gaggagcaga tcgtgttgaa aacgacggcc
     2521 tgcgggtttt tctgctgaat tttccggacg atttcccggt agtggtcgct gtcctgcgtg
     2581 gtctgggcca caatgcagac cttttccagc gcggggattt tttccgcgtc ttccagcgtc
     2641 gagacaacaa tgccccggcc ttcggtgtag ccccacaggc cgtccacctc cggatgttcg
     2701 cggtctccgg cgatgatcac cgtgtagtcc agcgccgtgt gttttttaat gatggcctgc
     2761 acgtagccga ccttcggaca ggtggcgtca atgattttaa cgccgctttc cttgattttc
     2821 tttcgctcac cgggcgcgat gccgtgcgcc cggatgatca gcagggcgtt ttgacggtcg
     2881 ggaatttcat cgatgctttg gatggccgta atgccccggc ttttcaagag ttcgatggtt
     2941 tgcgggttgt ggatcagggg gccgtaggtg tagatctttc ggccggtttc atgctgggaa
     3001 acgtccagaa ccgtgtccac cgcccggcgg acgcccatgc agaaccccgc tgttttggcc
     3061 agtttaattt ccatgtccgt tgatcttccg cgccaggata aaaaactcct tgttgcctgc
     3121 cggtcccaaa aggggcgagg cgcaggtccc ttccacgcgc aagccgaggg attgacaaag
     3181 cgaggcgatt tcgcgaacca cccgctcctg aaccgccgcg tcttccacca cgccgttttt
     3241 gccgacttct tccctgcgcg cttcaaactg cggtttgatg agagcgagga tccgcgcgga
     3301 atccccgagg tgcggcagga ctgcgggaat cacggttgca agcgagatga aagaaacgtc
     3361 gatgacggcc agcgacggtt tttcagtaag ccccgcgccg ttgtaatggc ggatgttcgt
     3421 gcgttcgatg accgtaacgc gcggatcttc ccgcagcttc caggccagct ggccgtaggc
     3481 gacatccacg gcgaacactg ctcttgcgcc cgcctgcagg aggcagtcgg tgaagccgcc
     3541 ggtggatgcc ccgacatcca gagcagccat tccttctacg ttcagcgaaa aaaaatccag
     3601 cgcccccttc aacttcaatc cgccgcggct gacgta
//
LOCUS       DAOSRM010000144         3542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_23754_length_3542_cov_98.338113, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000144 DAOSRM010000000
VERSION     DAOSRM010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23754_length_3542_cov_98.338113"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1346
                     /locus_tag="PLA58_10140"
     CDS             81..1346
                     /locus_tag="PLA58_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOR63193.1"
                     /translation="MDKRFNDLILAAIKQRISDLHITGGYPLIFRRDGVIHSDKNMKW
                     THTEIDKLVKEMLSDRQLFTLRRRWSVDFAMSLQEVRIRINAFYTTRGLSLAIRLLPE
                     TIPDVNALNLHPSLNQIRDLRAGMVLVCGSTGSGKSTTVAAIIEEINRTRPVHIITIE
                     DPIEYRFKPKKAFIEQREVGIHVPSFEQGLLDSLRENPDVIFVGELRDPDTIKTALTA
                     AESGHLVFGTLHATDAEDAIYRINNCVSGEGQEIIRYQFASSFSWLIIQQLIHIEKAG
                     FRVPVLSILRGSSSVRSSIRENKLTQLETIMHTGRNEGMFTMELYRKEFIDRLPSFNH
                     PYKSFGMGADSSKEKDYVSSLIDPNAVQNVVYTANLDDALIKPEDKRKEDDWQQYVVL
                     EDHDVDLKDIFEQYDHSDNRFNSNGSKDK"
     gene            1502..2593
                     /locus_tag="PLA58_10145"
     CDS             1502..2593
                     /locus_tag="PLA58_10145"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOR63194.1"
                     /translation="MAKYLYNEEAVANLKLGDTSVLDRLVDFESARAAHLKKEHAKKD
                     KRMTIGEAVATYVQDGDIITDGGFSYVRTPHQAFYEIIRQGKKNLQMIGAPNTNQSFF
                     IAYGLVTHSHSSYTGAEMRGIDKVYDRALKAGKITILSEWSHGGVAQGFKAAQLGVPG
                     VFSKQMLGSDLVRYNPYLKVVQHPLKADKDPSVFVPALYPDCAIIHCHAADKYGNAYI
                     YGPAVNDLAVAAAARKLIITAEEIVPESDIRYNKQGQIIPFFYADAVVEMPFGALPGS
                     MPGCYYWPRQWWEKCMRFGGVSEENNLAFCNEWIMGTKNQFEFVDKLGGAKWIAEARR
                     QTKAAEADNEDIDFSYEEYTLTHDSGIYY"
     gene            2608..3399
                     /locus_tag="PLA58_10150"
     CDS             2608..3399
                     /locus_tag="PLA58_10150"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOR63195.1"
                     /translation="MRDLNAPANPLETLAYIIAQQIEDHSIVYIGTGIPMVAGILAKK
                     THAPNITLVYESGGQDPIEGDMPWSVGCPMTWRKSPTIMEMSYSFAQCYNGYVDIGFL
                     GFAQVDMYGNINTHMIGKDYHHPKVRLTGSGGNNDLSSLTEKMVLVGLQTPDKFPERV
                     DFITSVGWLEGGDSRREAGLIGNGPAAVISPWGVYDFHPVSKRMRVKSLTPGVTFELA
                     QQLTGFELLKPEGAIPETKLVTPEALKVIRAEVDPRGVFTVMPTQ"
BASE COUNT          872 a          946 c          970 g          754 t
ORIGIN      
        1 ttaagatttc tcgtcgctca cgctcctcga aatgacattt ttcaaagctc tcggcatacc
       61 ggcaaaaggt tatggtttct atggacaagc gtttcaatga tctgattctt gcggcgatca
      121 aacagagaat ttcggatctg catattacgg gaggttatcc gctgattttc cgaagggacg
      181 gcgtgattca ttccgataaa aacatgaaat ggacgcacac ggaaatcgac aagctggtca
      241 aggaaatgct gagcgaccgc cagcttttca ccctgagaag gcgatggtct gtcgatttcg
      301 cgatgtctct tcaggaagtg cggatcagga tcaatgcgtt ctacacgacg cggggactga
      361 gcctggccat ccgcctgctt cccgaaacga ttccggacgt gaacgcgctg aacctgcacc
      421 cctccctcaa ccagattcgc gatcttcggg cgggaatggt tctggtctgc ggcagcacgg
      481 gatcgggcaa atccaccacg gtcgcggcga ttattgaaga aatcaaccgg acccggccgg
      541 tccatatcat cacgattgaa gacccgattg aataccggtt caagcccaag aaagcgttta
      601 ttgaacagcg ggaggtgggc attcacgtgc cgtctttcga gcagggactg ctggattccc
      661 ttcgtgaaaa tccggacgtc atttttgtcg gggagcttcg ggacccggat accatcaaga
      721 ccgccctgac ggcggccgag tccggccatc tggtgttcgg aaccctgcac gccacggacg
      781 cggaagacgc catttaccgc atcaacaatt gcgtttccgg agagggccag gagatcattc
      841 gctatcaatt tgcctcttcg ttttcctggc tgatcatcca gcaactgatt catatcgaga
      901 aggcgggctt ccgcgttccg gtcctgtcca tcctgcgggg ttcttcatcc gtccggagca
      961 gcatccgtga aaataagctg acgcagttgg aaacgattat gcataccggc cggaatgaag
     1021 gcatgttcac gatggaactc taccgaaagg agtttatcga tcgccttcct tcctttaacc
     1081 acccgtacaa gagtttcggc atgggtgcgg attcgtccaa ggaaaaggac tatgtttctt
     1141 cactgatcga tcccaatgcc gttcaaaacg ttgtttacac cgccaatctc gacgatgcgt
     1201 tgatcaagcc tgaggacaaa cgcaaggaag atgactggca gcagtatgtc gttctggaag
     1261 accatgacgt tgatctgaag gatattttcg agcagtacga tcactccgac aaccggttca
     1321 attccaacgg ctcaaaagat aagtaagcaa agacatcaag aatatcaaag tctttcgagc
     1381 accactgccg gacaacactt aaaagttaac tcttgacctt aacgggccgg gatagtaatc
     1441 tttttaaaag tttataatgt gagggaacac atggggttaa tacaaagagg aggagaaaga
     1501 gatggcaaaa tatctctaca acgaggaggc cgtcgcgaat ctgaaactcg gcgatacatc
     1561 cgttctggat cgcctggttg attttgaatc agcgcgcgcg gcgcatttaa agaaagaaca
     1621 cgccaagaag gacaagcgga tgaccattgg cgaggcggtt gcgacgtacg tgcaggatgg
     1681 agacatcatc acggacggtg gtttttctta cgtacgcaca ccccatcagg cgttttacga
     1741 aatcatccgt caggggaaaa agaacctgca gatgatcggc gcgcccaata ccaatcagag
     1801 cttttttatc gcctacgggt tggtgacgca ttcccacagt tcctatacgg gcgcggaaat
     1861 gcgcgggatt gataaggttt atgaccgggc gctcaaagcc ggcaagatca cgattctttc
     1921 cgaatggagt cacggcggcg tggctcaggg attcaaggcc gcacagctcg gcgttccggg
     1981 ggttttcagc aaacagatgc tgggcagcga tctcgtccgc tacaacccgt acctcaaggt
     2041 cgttcagcat ccgctgaagg cggacaagga tccgtcggtg tttgttccgg ccctgtatcc
     2101 cgattgcgcg atcatccatt gccacgcggc ggacaagtac ggcaatgcct acatttacgg
     2161 ccccgccgtc aatgatctgg cagtagcggc ggcggcgcgc aaactgatca tcacagcaga
     2221 ggagatcgtt cctgaaagcg acatccgcta caacaaacag ggacagatca tcccgttctt
     2281 ctacgccgat gccgtggtcg aaatgccctt tggcgcgctt cccggcagca tgcccggctg
     2341 ttactactgg ccgcggcagt ggtgggagaa gtgcatgcga ttcggcggcg tctcggaaga
     2401 aaacaacctg gcattttgca atgaatggat tatgggcaca aagaatcaat ttgaatttgt
     2461 ggacaagctg ggaggcgcca aatggatcgc cgaggcccgc cgccagacga aagccgcgga
     2521 agccgacaat gaagacattg atttctcata cgaggaatat accctgacgc atgattcagg
     2581 aatttactat taggacagga gcagattatg cgagacttaa acgcaccggc caatccgctg
     2641 gagacattgg cctatattat cgcgcagcag atcgaagacc attcgattgt ttatatcggc
     2701 acgggaatcc ccatggtggc ggggattctg gccaagaaaa cccacgcccc caacattacg
     2761 cttgtgtatg aatccggcgg gcaggacccg atcgaaggcg atatgccctg gtcggtcggc
     2821 tgtcccatga cctggcgcaa atcccccacc atcatggaga tgtcctattc gttcgcgcag
     2881 tgctacaacg gctacgtgga catcgggttt ctggggtttg cccaggtgga tatgtacggc
     2941 aacatcaaca cccacatgat cggcaaggac taccatcatc ccaaagttcg cctgaccggc
     3001 agcggcggca acaacgattt gtcgtcgctc accgagaaga tggttctggt cggactccag
     3061 acgccggaca agttcccgga gagggtcgat tttatcacgt cggtcggctg gctcgaaggc
     3121 ggcgattccc gcagggaggc gggcctcatc ggcaacggcc ccgccgcggt gatcagtccc
     3181 tggggtgtct atgattttca tccggtaagc aagcgcatgc gggtcaaatc cctgacgccc
     3241 ggcgtcacct ttgaactggc ccagcagctc accggttttg agcttctcaa acccgaagga
     3301 gcaattcccg aaaccaaact ggttacgccc gaagccttga aggtgatccg cgccgaagtg
     3361 gatccgcggg gcgtcttcac cgtcatgccg acgcagtaaa ggcccaaaaa aaagatgctg
     3421 cagcaaccgg caatgaaatc gcgggtccgc acgaaaaggg cccgcgattt ttttcatggc
     3481 tgaaaatcgg cggtctcgta aaaagtaatc caagtcgtcc ccccggcgaa ggccggggcc
     3541 ta
//
LOCUS       DAOSRM010000145         3538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_23785_length_3538_cov_116.701981, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000145 DAOSRM010000000
VERSION     DAOSRM010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23785_length_3538_cov_116.701981"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1129)
                     /locus_tag="PLA58_10155"
     CDS             complement(146..1129)
                     /locus_tag="PLA58_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOR63196.1"
                     /translation="MQKKYEALSEQVTLTRTLMARSVIYKYLNRTNLICYSELSRLLG
                     CEAFVKHENHNPTGSFKIRGALNFFHHMSREELEAGILVATRGNHGLAMAWAGQWFHV
                     PCTVVVPEDNNPEINRIIESYGAELIVHGEDFYDAQSYCEELVDSAGYYYVQQGNEPE
                     ILNGLATMGLEILEDLPQVDVIICPIGGGAGCASLLKTVRAVNPAVEIIGVEPQNAPS
                     FYESWKKGEIVTLDDAHTVADGLAARSVFHLPYVILKNTISDVVLLTEDEILEGIKMA
                     LTATHNVAEAAGAVSLMAAYKIRDRLAGKRVVMIMSGGNLKMDNLRKAIGC"
     gene            complement(1227..2519)
                     /locus_tag="PLA58_10160"
     CDS             complement(1227..2519)
                     /locus_tag="PLA58_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOR63197.1"
                     /translation="MRLFYNMLLAVAAVPGLLFFLLKMLLTGKYRHSFLQKLGARQDH
                     LPAGIAPGRRVWVHAVSVGEVTAAAPVVAALKAKRPDVRIIFSTSTETGQGMAQRLVK
                     DADAFIYFPLDLPFSVGHMLDVVRPEVFVPVETELWPNFLAACRRRNISTVMVNGRLS
                     SRSFSRYRRTRFFWRQVLKNVDAAGMISEVDASRIAGMGMDPAKISILGNAKYDALAA
                     MASPALREDIARRFHARPHEKFLVAGSTHPGEEDVVIGVYRELLARDPDFRLIVVPRH
                     VERAGDVVRLLRESGFPDVITATQMSNGQSRRDEKVIVVDVIGELFKTYALATVVYCG
                     GSLVPRGGQNILEAAAWGKVIFYGPSMDDFSSEAALLEAAGAGIRIHSARELLEKMLQ
                     THADPQDAQARGERGRAVVLANRGAAQRYAEMVIRYIG"
     gene            2749..3408
                     /gene="rpe"
                     /locus_tag="PLA58_10165"
     CDS             2749..3408
                     /gene="rpe"
                     /locus_tag="PLA58_10165"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOR63198.1"
                     /translation="MKLIAPSILSADGGRLAEEIAAVEKAGADWIHVDVMDGHFVPNI
                     TMGPAVIKALRKTTRLPFDVHLMIQNPDDYVEAFADAGADYITVHVEAATHLHRTLGQ
                     IKKAGKKAGVSLNPSTPLGFLEEILPDLDLLLIMSVNPGFGGQQFIPGSLAKIRRAAD
                     LLASLPQKPLLEVDGGVNLANIAAIAGAGADVLVAGAAVFGTPDYAQTIAALKAEANK
                     S"
BASE COUNT          735 a         1075 c          965 g          763 t
ORIGIN      
        1 tagccgttgg gttcgggcgg aaagcgggtc gccacgcgcc cttcgtgttt gccggcgcga
       61 aggccttcgt cgatgatgtc ccggataaaa tcgctcgggg cgggggttga cggttcagtc
      121 ataaaacaaa aaactccttt aaaaattaac agccgatggc ttttctcaaa ttgtccattt
      181 tcaggttgcc gccggacatg atcatgacca cccttttgcc cgccagccgg tccctgattt
      241 tgtaagcggc catcagggaa acggcgcccg cggcttcggc cacgttgtga gtggccgtaa
      301 gcgccatttt gattccttcc agaatttcat cctcggtcaa cagcaccacg tcgctgatgg
      361 tgtttttgag gatgacatag ggcagatgga aaacgcttcg cgcggcaagg ccgtcggcca
      421 ccgtatgggc gtcatccagc gtgacgatct ctcccttctt ccaggactcg taaaaggaag
      481 gcgcgttctg gggctccacg ccgatgattt cgacggcggg attgacggcg cggacggttt
      541 tcagcaggga cgcgcacccc gcgcctccgc cgatgggaca aatgatcaca tcgacctgcg
      601 gcaggtcctc cagaatctcc agtcccatgg tggccaggcc gtttaaaatt tccggctcgt
      661 tgccctgctg aacatagtaa tatccggcgc tgtccaccag ttcctcgcaa taggactggg
      721 cgtcgtagaa gtcttctccg tggacgatga gctccgcgcc gtagctttcg atgatgcggt
      781 tgatttccgg gttgttgtct tccggcacga ccaccgtgca gggcacgtgg aaccactggc
      841 ccgcccaggc catcgccagg ccgtgattgc cccgggtcgc caccagaatg ccggcttcca
      901 gctcctcgcg ggacatgtga tggaaaaagt tcagggcgcc ccgaatcttg aaagacccgg
      961 tcggattgtg gttttcatgc ttgacaaagg cttcacagcc cagaagcctg gagagctccg
     1021 aatagcagat caggttcgtc cggttgaggt atttgtagat caccgatctc gccatcaggg
     1081 ttcttgtcag agtgacctgc tcgcttagcg cttcgtattt tttctgcatc aataacctcc
     1141 ctggtcttag cagcccgttt cttttaaacc actttttcgc ccggcgtcaa gcaaacttca
     1201 cttttattcc cttcagcctt cggtcttcag cctatatacc tgataaccat ttccgcgtag
     1261 cgttgcgccg cgccccggtt ggccaggaca accgcccggc cccgttcgcc gcgcgcctgt
     1321 gcatcctgcg gatcggcatg cgtttgcagc attttttcca gcagctcgcg ggcgctgtga
     1381 atacgaatgc cggcgcccgc cgcttccagg agcgcggctt ccgagctgaa atcatccatg
     1441 gaggggccgt aaaaaatcac ttttccccag gcggccgctt ccagaatgtt ctggccgccc
     1501 ctcggcacga gcgagccgcc gcagtaaacc accgtcgcca gcgcatacgt cttgaaaagc
     1561 tcgccgatca catcgacgac gattactttt tcatcccggc gggactgccc gttgctcatc
     1621 tgcgtcgccg tgatgacatc ggggaatccc gactcccgca ggagccggac cacatcgcct
     1681 gcccgttcga catggcgcgg cacgacaatc agccgaaaat ccggatcgcg cgccagcagt
     1741 tcccgataga ccccgatcac aacgtcttct tcgcccggat gcgtgcttcc cgcgacaaga
     1801 aatttctcgt ggggacgcgc atgaaagcgg cgggcgatat cctcccgcag ggccggcgat
     1861 gccatcgccg ccagggcatc gtacttggcg ttgcccaaga tgctgatctt tgccggatcc
     1921 atgcccatgc cggcaatccg ggaggcgtcc acttcggaaa tcatcccggc ggcgtccaca
     1981 tttttcagaa cctgacgcca gaaaaaccgc gtccggcggt atctggaaaa ggagcgggag
     2041 gaaagacggc cgttcaccat cacggtggag atattccgcc tgcggcaggc agccagaaaa
     2101 ttgggccaca gctccgtctc caccggaaca aacacctccg gacggacgac atccagcata
     2161 tgaccgacgc tgaacggaag atcaagaggg aaataaataa acgcgtccgc gtccttcacc
     2221 agtctttgcg ccatgccctg gccggtttcc gtcgatgtcg aaaaaataat ccgaacgtcc
     2281 ggccttttcg ccttcaacgc cgcgacgacc ggcgcggcgg cggtcacctc tcccacggaa
     2341 accgcgtgaa cccagacgcg ccgtccgggc gcgatgcccg ccggcagatg atcctgacgc
     2401 gcgcccagct tctggagaaa actgtggcgg tacttgcccg taagcaacat tttgagcaga
     2461 aagaacagca gtcccggaac cgccgcgacc gccagaagca tgttatagaa caatctcatg
     2521 cgcgcatctt tgattttttc agctttggtg tcaagaaaat ccttcggata ggttattagg
     2581 ctgaagactg aaggctaaag gttattttga ggggcgcttc gcgcccattc ctctttccct
     2641 tcggacttca gccttcagcc ttcggtcttc tttcgatcct cggccttctt tcgattgaca
     2701 gtgcccggtc gttggtatat tgaagaacaa cgatttggag aaaatcctat gaaactgatt
     2761 gctccttcga ttctttccgc cgacggcgga aggctcgcgg aagaaatcgc cgcggtggaa
     2821 aaagccggcg cggactggat ccacgtcgat gtcatggacg ggcattttgt ccccaacatc
     2881 acgatggggc cggccgtcat caaggctttg cgaaaaacca cccggctgcc ctttgacgtg
     2941 cacctgatga ttcaaaatcc ggacgattac gtcgaagcct tcgcggacgc gggcgcggat
     3001 tatattaccg tccatgtgga ggcggccacg catctgcacc gcacactcgg acagattaaa
     3061 aaagccggga aaaaagccgg tgtttcattg aacccgtcca caccgctggg ttttctggag
     3121 gaaatcctcc ccgacctcga cctgcttttg atcatgtcgg tcaaccccgg attcggcgga
     3181 cagcagttca ttccaggctc tcttgccaaa atcagacggg ctgctgatct gctcgcctcc
     3241 ctgccgcaaa aacctctgct ggaagtggac ggaggggtga atctggccaa tatcgccgcc
     3301 atcgcgggag cgggcgcgga tgtgctggtc gcgggcgcgg cggttttcgg aacgcccgac
     3361 tacgcacaaa ccatcgccgc cctcaaagcc gaagccaaca aatcataaat ttctttaatg
     3421 gggcttttgc ggggaacggt cgcgaccgtt ccctgcaatt cccttgaatt catcaattag
     3481 aacgatgggt tgcaacccat tgttctcggg gaaagcaaac tgacgaaaat catttctt
//
LOCUS       DAOSRM010000146         3518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_23973_length_3518_cov_99.835692, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000146 DAOSRM010000000
VERSION     DAOSRM010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23973_length_3518_cov_99.835692"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLA58_10170"
     CDS             <1..216
                     /locus_tag="PLA58_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOR63199.1"
                     /translation="DLRDYGIGAQILADLGVHKMRMMTNNPKKIVGLEGYAIAVTQRV
                     PIEIPPNENNIRYMTTKKKKMGHILEL"
     gene            313..783
                     /gene="ribE"
                     /locus_tag="PLA58_10175"
     CDS             313..783
                     /gene="ribE"
                     /locus_tag="PLA58_10175"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOR63200.1"
                     /translation="MPKIIEGKIVAKGMKFAIAASRFNDFISGRLIDGAVDTLVRAGA
                     DEKDIAIYKVPGAFELPLAAKKLAKSQRFDAVICLGAVIRGATPHFEYVSAEVSKGIA
                     NVGLDAEIPVAFGVLTTDTIEQAIERAGAKSGNKGADAAMAAIEMVDLFKKMNP"
     gene            839..1294
                     /gene="nusB"
                     /locus_tag="PLA58_10180"
     CDS             839..1294
                     /gene="nusB"
                     /locus_tag="PLA58_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOR63201.1"
                     /translation="MQERRKAREVALQVLYSLNFVTLDTESAQELFWGNFVAPKSARE
                     FAATLISGAWSHREELDRLIADSSDNWSLGRMSKVDISILRMAVFEFLYCDDIPPKVS
                     INEAVDLGKAFGSENSGSFINGILDALHLKLQKKHADHAVHTATRPAKA"
     gene            1248..1823
                     /locus_tag="PLA58_10185"
     CDS             1248..1823
                     /locus_tag="PLA58_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63202.1"
                     /translation="MQITLSTQPPDRQKHKCLVLGFFDDEKPPRGICGLMDWRLNSLI
                     SREIKQGHIRGEFKEQVAIPWPERIDAEMLFLFGLGGLADLSYDRIYEAAYAVMTAAG
                     RMKLWDFSFDLPGDGRSMLSPAGVLEAMLTGFFDCLSEDISRLTHTNICLVTSPQNLQ
                     EVALGMKRFNENVRHLGSVDFSALQARLAGY"
     gene            complement(2092..2499)
                     /locus_tag="PLA58_10190"
     CDS             complement(2092..2499)
                     /locus_tag="PLA58_10190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR63203.1"
                     /translation="MIMKKMPARVESLHELLTFIRAEAKKRGFSESALNRIELVAEEA
                     LVNVFVHGYAKGQGQVEVRCLVADDPSLTIEIHDTGVAFDPLSLADPDVQSDLTKRKI
                     GGMGVFFIRKMTDRVAYRREGNSNILAMTFLNR"
     gene            complement(2518..2853)
                     /locus_tag="PLA58_10195"
     CDS             complement(2518..2853)
                     /locus_tag="PLA58_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOR63204.1"
                     /translation="MEIVVTNESDIAVFAITGRLDAVSVPQLEERLNQWFEQPGKKLI
                     FDLQGLDYISSAGLRVFLTTAKKMKARDGKLCMARLRDNVKDVFTISGFIALIPAFDT
                     LGAAQDAIK"
     gene            3041..>3518
                     /locus_tag="PLA58_10200"
     CDS             3041..>3518
                     /locus_tag="PLA58_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOR63205.1"
                     /translation="MIYNEEFETLPREALEALQFKRLQQVASRLYHTVGFYKKSFDRA
                     GVTPESLKSLDDLKRFPFTTKQDLRDNYPFGLFAAPMSNVVRLHASSGTTGASTVVGY
                     TKRDIDTWAELMGRCFVAAGLTKNDVIHNAYGYGLFTGGLGAHYGAEKIGASVVPMS"
BASE COUNT          840 a          933 c          979 g          766 t
ORIGIN      
        1 gatctgcggg attacggcat cggcgcgcag attctggcgg atctgggcgt gcacaagatg
       61 cggatgatga ccaacaatcc caagaagatt gtcggcctgg aaggatacgc cattgccgtg
      121 acccagcggg ttccgatcga aattcctccc aacgaaaata acatccggta tatgaccacc
      181 aagaaaaaga aaatggggca tattctggag ttgtaaaaaa gcgcagcggc gccaaaaatc
      241 ggacaagggg ctgtaccccc tgagggcggc aagtcctctt gctccaataa acaaaaggat
      301 ggtgctttaa tcatgccgaa gatcattgaa ggaaaaattg tcgccaaagg gatgaaattc
      361 gcgatcgccg caagccggtt caacgatttc atcagtggac gccttatcga cggcgcggtg
      421 gacacgctgg tccgggcggg agcggatgag aaagacatcg ccatttacaa ggttcccggc
      481 gctttcgaac tgccgcttgc agcgaaaaag ctggccaaaa gccagcgctt tgacgcggtc
      541 atctgcctgg gcgcggtcat ccgcggcgcg acgccgcatt ttgaatacgt cagcgcggaa
      601 gtatccaagg gaatcgccaa cgtggggctg gacgcggaaa ttccggtggc cttcggcgtt
      661 ctgacgacgg ataccatcga acaggccatt gaacgggcgg gcgccaagtc cggcaataaa
      721 ggcgcggacg cggcgatggc ggccattgaa atggtggacc tgttcaaaaa gatgaacccc
      781 taaggcgaaa agcaggacaa cgccggcatg atccggcatt gcgacgtttg aggattcgat
      841 gcaggaacgg agaaaggcgc gcgaagtcgc cttgcaggtt ctttatagtt tgaattttgt
      901 cacccttgac acggaaagcg cccaggagct tttctggggg aattttgtcg cgcccaagtc
      961 ggccagggaa tttgcggcca cgctgatttc cggcgcctgg agccaccggg aagaactgga
     1021 ccggctcatt gccgatagtt cggacaactg gtcgctggga aggatgtcca aggttgacat
     1081 cagcattctg cgaatggcgg tttttgagtt tctctactgt gacgacattc cgcccaaagt
     1141 cagcattaat gaagcggttg acctgggaaa agcgttcgga tcggaaaatt ccggctcttt
     1201 tatcaacggc atcctggatg ccctccattt gaaactacaa aaaaaacatg cagatcacgc
     1261 tgtccacaca gccacccgac cggcaaaagc ataaatgtct ggttttgggt tttttcgacg
     1321 atgaaaagcc cccgcgcggc atttgcgggc tgatggactg gcgtctcaac agcctgattt
     1381 ccagggaaat caaacaggga cacatccggg gcgagttcaa ggagcaggtg gcgattccct
     1441 ggccggagcg cattgacgcg gagatgcttt ttcttttcgg cctgggcggt cttgccgatc
     1501 tttcctatga tcggatttac gaggcggcct atgcggtgat gaccgcggca ggcagaatga
     1561 agctgtggga tttttcgttt gatctgcccg gggacggccg ttcgatgctc tctccggccg
     1621 gtgtgctgga ggccatgctg accggttttt ttgactgcct gtcggaggat atcagccggc
     1681 tgacccatac caatatctgt ctggtcacat cgcctcagaa cctgcaagag gtcgccctcg
     1741 gcatgaagcg attcaatgaa aatgtgcggc acctgggatc ggttgatttc agcgcgcttc
     1801 aggcgcgcct cgccggatat tgatcagaca atataacacg ccgtaaaaat taccgatcac
     1861 gacccgcctt tgcgaaaagc aaagtgacaa agccgtcctg ccggagatga tgtatggaga
     1921 acttttcatc attacacttt tgtcatcgcg aggagcgaag cgacgtggcg atctcttgaa
     1981 atgatcaaat gcttatgaga ttgcctcact tcgttcgcaa tgacgaagag acatcatgca
     2041 aatgtttcga atggatggat gaaaaagggt gaaggcgaag gcccgccgga ttcagcggtt
     2101 cagaaacgtc atggccagga tattgctgtt tccttcccgg cgataggcca cccggtcggt
     2161 catcttgcgg atgaagaata cgcccatgcc gccgatcttg cgtttcgtca gatccgattg
     2221 aacgtccgga tcagccagcg acaggggatc gaaagccacc cccgtgtcgt ggatctcaat
     2281 cgtcagcgac ggatcgtccg caaccaggca gcgcacttca acctgtccct ggcctttagc
     2341 gtagccatgc acgaacacgt tgaccagcgc ctcctccgca accagttcaa tccggttcag
     2401 ggcgctttcg gaaaaccccc gcttttttgc ctcggcccga atgaaagtca gcagttcatg
     2461 caagctttcc acccgggcgg gcattttctt cataatcata gacgaatccg gccaacgcta
     2521 cttgatggcg tcctgagccg ctcccagagt gtcaaatgcg ggaatcagag cgataaaacc
     2581 cgagatggta aaaacgtctt tcacattgtc ccggagtctg gccatgcaca atttcccgtc
     2641 acgcgccttc attttcttgg ccgtggtcag aaagactctc agaccggcgc tgctgatata
     2701 gtcgagtccc tgcagatcga aaatcagctt ttttccgggc tgttcaaacc actgattgag
     2761 ccgctcctcc agttggggga cggaaacggc atccagtctt cctgtaatgg caaaaacggc
     2821 aatatctgac tcatttgtaa cgacgatttc cataggtgtg ctccttatcg tgatggctcg
     2881 aacctgaaaa aaagcattcg gacggcgcga gtatagaaag aacatcggcg tctgtcaata
     2941 aattttaagg gttacctgaa atcttaaaac tttcgttttg agcaaagttt atgctatagg
     3001 aaggcaaaca tttctgaaag ccggaggaga accgaacccc atgatctaca atgaagaatt
     3061 tgaaaccctg ccccgggagg cgctcgaagc gctgcagttc aaaagactcc aacaggttgc
     3121 ctcgcggctt taccataccg tcggttttta caaaaagtct tttgaccggg cgggtgtgac
     3181 gccggagagc ctgaaatccc tcgatgatct gaagcgtttt cctttcacca ccaagcagga
     3241 tttgcgggac aattatccct tcgggctttt tgccgcgccg atgagcaatg tcgtgcgcct
     3301 gcacgcctcg tccggcacaa ccggcgcatc caccgtggtc ggctacacga agcgcgacat
     3361 cgacacctgg gcggagctca tggggcgctg ttttgtggcc gcgggcctca ccaagaacga
     3421 cgtcattcac aacgcttacg gctacggcct gttcaccggc ggtctgggcg ctcattacgg
     3481 cgcggaaaaa atcggcgcca gcgtcgtgcc gatgtccg
//
LOCUS       DAOSRM010000147         3510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_24042_length_3510_cov_100.322721, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000147 DAOSRM010000000
VERSION     DAOSRM010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24042_length_3510_cov_100.322721"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLA58_10205"
     CDS             complement(<1..261)
                     /locus_tag="PLA58_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOR63206.1"
                     /translation="MTTPGEILHRYRIRPDKRRGQSFLTDLNTLHKIAAAGKITPEDV
                     VVEIGAGFGVLTQNIAPCAGRLIAVEIDLRLVEILRKELAQFD"
     gene            complement(269..1294)
                     /gene="tsaD"
                     /locus_tag="PLA58_10210"
     CDS             complement(269..1294)
                     /gene="tsaD"
                     /locus_tag="PLA58_10210"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOR63207.1"
                     /translation="MFVLGIESSCDETAAAVVRDGKLLSSVIASQIKDHSAYGGVVPE
                     IASRKHIEAITPVIEQALRDAGAAFRDMEGIAVTRGPGLIGSLLVGLSTAKALAYALG
                     IPFVGVNHLEAHIMASLLGEKKPRFPFAALVVSGGHTNVYLVKDYHRFALLGQTRDDA
                     AGEALDKAAKLLDLGYPGGVVIDRMAREGNPDLFCFPRAMKDSPDFSFSGLKTSLLVM
                     MKKRGKAFVQNEIPDVAAGYQEAIVDVLVEKTLRAARENDLSQIVVCGGVAANSRLRG
                     KFSEAAIQEGRELFFPPMILCTDNAAMVAALGEVMLENGRSDSLDLNAVSRWPLVLPP
                     PNKDGRG"
     gene            1406..2995
                     /gene="nadB"
                     /locus_tag="PLA58_10215"
     CDS             1406..2995
                     /gene="nadB"
                     /locus_tag="PLA58_10215"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOR63208.1"
                     /translation="MEIKTDFLIIGSGIAGLSLAIKVAQLGSVAIVTKKEKSESNTNY
                     AQGGIAAVTDKTDSFEEHISDTLECGAGLCRKDVVEFVVREAPPRIQELIEWGVNFTK
                     SEEPPHLYDLGQEGGHHRRRVLHAKDLTGQEIERALHEKVAALKNVRIYENHIGIDLI
                     LQKDPQGRTVNCLGAYVLDINQDEIHTYRAKYTILSTGGAGKVYLITTNPDIATGDGI
                     AMAYRAGAKIANMEFIQFHPTCLFHPEAKAFLISEAVRGEGGILKLKNGSTFMEKYHP
                     MKSLAPRDVVAKAIDNEIKQSGDEYVLLDISHHSREFLLNRFPNIYGKCLEFGIDMAR
                     DPIPIAPAAHYICGGVAVDHCGKTSIDNLFACGEVSCTGLHGANRLASNSLLEALVYS
                     HRVFTKIAKSFRQTPMSAVPIRPWDPGDTSASDDNIVITNNWDEIRRCMWNYAGIVRS
                     DKRLARAERRIDMVQREIHEYYWNYKVTKDLIELRNITTVAKLIVKSALARKESRGLH
                     FTMDYPETKDDFLKDTILVKK"
     gene            complement(3058..>3510)
                     /locus_tag="PLA58_10220"
     CDS             complement(3058..>3510)
                     /locus_tag="PLA58_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOR63209.1"
                     /translation="RLVRSYALAALEDVALWHERDISHSSVERVIAPDATIALDFMLG
                     RFTGLMDRLVVYPDRMLANLNMTHGVIFSQRVLLSLIEKGTTREDAYAIVQENAMKSW
                     QEGTEFRKLLGQDERVQKHLNAADLDVIFDVNHFLKHLDYIFERVFGG"
BASE COUNT          813 a         1070 c          935 g          692 t
ORIGIN      
        1 gtcgaattgg gcaagctctt tccggaggat ttccaccagt ctgaggtcaa tttccacagc
       61 gatcagccgg ccggcgcagg gcgcaatatt ttgggtcaaa acaccgaagc cggcgccgat
      121 ttccacaaca acgtcttccg gggtgatctt tccggctgcc gcgattttat ggagcgtgtt
      181 gagatccgtc agaaacgact gtccgcgtct tttatcgggc cgaatcctgt atcggtgaag
      241 aatttctccg ggtgtcgtca tgtctcgttc agccccgccc atctttgttc ggaggcggca
      301 aaaccagagg ccagcgggac accgcgttca gatccagaga atcgctcctg ccgttttcca
      361 gcatgacctc tcccagggca gcgaccatcg ccgcgttgtc cgtgcacaaa atcatcggcg
      421 gaaaaaacag ctctctgcct tcctgaatcg ccgcctcgga aaacttcccg cgcaaacgac
      481 tgttggcggc aacgccgccg caaacgacaa tctgcgaaag gtcgttttcc cgggcggccc
      541 tgagtgtttt ttccaccaga acatcaacga tggcttcctg gtagccggcg gcaacgtccg
      601 gaatctcgtt ttggacaaat gccttccccc gcttcttcat cataaccagc agagatgttt
      661 tcaacccgct gaagctgaaa tccggcgaat ccttcatcgc ccgggggaaa caaaacaaat
      721 cgggatttcc ttccctggcc atgcggtcga tcacgacgcc ccccggatag cccagatcga
      781 gcagcttggc cgccttgtcc agcgcctcgc ctgccgcatc gtccctggtc tggcccagca
      841 gcgcaaaccg gtgataatct ttcaccagat acacattcgt atggccgccg gaaaccacga
      901 gggcggcaaa cggaaaccgc ggttttttct cccccagcag agacgccatg atgtgcgctt
      961 cgagatggtt gacgccgaca aacggaatgc ccagcgcata ggccagcgcc ttggcggtgg
     1021 aaagcccgac caagagcgat ccgatcagcc ccggcccgcg ggtgacggca atgccttcca
     1081 tatcccggaa ggcggcgccc gcgtcccgga gggcctgttc aatcacggga gtaatcgcct
     1141 cgatatgttt gcgcgacgcg atctccggca ccacgccccc gtaagcgctg tgatccttga
     1201 tttgcgaggc aatcacgctg gagagcagct ttccgtcccg gacgacggcg gccgccgttt
     1261 catcacagga tgactcgatt cctaaaacga acaaatgaac tcctttgtca taattttctt
     1321 tacaaaccgc tgcaaaggct tatataaaag cccccggcat ttgtaaacaa acttttagcg
     1381 gcaggaatga tcgggctctt ctttcatgga aataaaaacg gacttcctca tcatcggcag
     1441 cgggatcgcg gggctcagcc tcgccatcaa agtggcgcag ctcggatcgg tcgccattgt
     1501 caccaaaaag gaaaaatcgg aatccaacac caactatgcc cagggcggca tcgccgcggt
     1561 aacggacaag accgacagct ttgaagagca catcagcgac acgctggaat gcggcgcagg
     1621 cctctgccgg aaggacgtcg ttgagtttgt cgtgcgggaa gcgccgccgc gcattcagga
     1681 gctgatcgaa tggggcgtca atttcacgaa atcggaagag cctccccacc tttatgacct
     1741 gggccaggaa gggggccacc atcgccgccg ggttcttcat gccaaagacc tcaccggcca
     1801 ggaaatcgaa cgggcgctgc atgaaaaggt cgccgcgctg aaaaacgtcc ggatttatga
     1861 aaatcacatc ggcatcgacc tgattttgca aaaagacccc caggggcgga cggtcaactg
     1921 tctgggcgcc tacgtgctcg acatcaatca agacgaaatt cacacctatc gcgccaaata
     1981 tacgatttta tcgacgggag gcgcgggcaa agtgtatctg atcaccacca acccggacat
     2041 cgccacgggc gacggcattg ccatggccta ccgcgccggc gcgaaaatcg ccaatatgga
     2101 attcatccag tttcatccca cctgcctgtt ccatccggaa gccaaggcgt ttctgatcag
     2161 cgaagccgtg cgcggcgaag gcggcatcct gaagttaaaa aacggttcga ccttcatgga
     2221 aaaataccat cccatgaaaa gcctcgcgcc gcgcgacgtg gtggccaaag ccatcgacaa
     2281 cgaaatcaag cagtccggcg atgaatacgt ccttctggac atctcgcacc acagccgcga
     2341 atttctcctg aaccggtttc ccaatatcta cggcaaatgt ctggagttcg gcatcgacat
     2401 ggcccgggac ccgattccca tcgccccggc ggctcattac atctgcggcg gtgtcgcggt
     2461 ggatcactgc ggcaaaacct ccatcgacaa tctgttcgcc tgcggggaag tttcctgcac
     2521 cggcctgcac ggcgcgaacc gcctggccag caactcgctt ctggaggcgc tggtttattc
     2581 acaccgggtc tttacaaaga tcgccaaatc cttccggcag actcccatga gcgccgtgcc
     2641 gatccgaccc tgggaccccg gcgacacttc ggccagcgac gacaatatcg tgatcaccaa
     2701 caactgggat gaaatccgcc ggtgcatgtg gaactacgcg ggcatcgtcc gttcggacaa
     2761 acgcctcgcg cgggccgaac gccggatcga catggtgcag cgggaaattc acgaatacta
     2821 ctggaactac aaggtcacca aggatctgat cgaactgcgc aacatcacca ccgtggccaa
     2881 gctgattgtg aaaagcgcgc tggcccggaa ggaaagccgc ggcctgcatt tcacgatgga
     2941 ttatcccgaa acgaaggatg acttcctgaa agacacgatc ctggtaaaaa aataacggct
     3001 gctggagttt tttgaatctt ccgcggggaa cggccgcgac cgttccctgc gtttcattta
     3061 accaccgaaa acccgttcaa agatgtaatc cagatgcttc aggaaatgat ttacgtcgaa
     3121 aataacgtcc aggtcggcgg cgttcagatg cttttgcacc ctttcatcct gcccgaggag
     3181 tttgcgaaat tcggttccct cctgccagga tttcatcgcg ttttcctgaa cgatggcata
     3241 ggcgtcttcc cgcgtcgtac ccttttcaat gagcgaaagc agaactcttt gcgaaaagat
     3301 cacaccgtgc gtcatgttga gattggcgag catccggtcc ggatacacca ccagcctgtc
     3361 catcaaaccg gtaaagcgcc ccagcatgaa atcgagcgcg atggtggcgt ccggcgcgat
     3421 cacccgctcc actgaggagt ggctgatgtc tcgctcgtgc cataaggcga catcttccag
     3481 ggcggccagg gcgtaggaac gcaccaggcg
//
LOCUS       DAOSRM010000148         3505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_24099_length_3505_cov_106.189275, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000148 DAOSRM010000000
VERSION     DAOSRM010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24099_length_3505_cov_106.189275"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLA58_10225"
     CDS             <1..186
                     /locus_tag="PLA58_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63210.1"
                     /translation="TRLRLKQLSKADLSLRQDLLNKTLSMAAQRDIWATGISARLKVC
                     ATLNEGLYARFMAFFPY"
     gene            complement(164..1138)
                     /gene="meaB"
                     /locus_tag="PLA58_10230"
     CDS             complement(164..1138)
                     /gene="meaB"
                     /locus_tag="PLA58_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOR63211.1"
                     /translation="MTQTTQKICAGDIRSASRLIRDLEDKRPEARLRLRQLFPHTGRS
                     FIIGITGSPGAGKSTLADALIGELRKRKKTVGVLAVDPSSPFSGGALLGDRVRMLRHA
                     EDEDVFIRSLATRGALGGLAHAAGDAIHVMEAMGKDVILVETVGIGQQELDIIHHAHS
                     IVVVLVPGMGDEIQTMKAGVMEIADVFVINKADRPGAKQLKTELTSLLNASPHGPRDW
                     IPPIVSIGNLYEPESFAKKTAELMDAVAKHHAHLVVSGQLAERTERKAVMEITDALRA
                     CILEPVIERLITDGELKKIVGKIQNRETDPYTEAEAIARRFLNTGKTP"
     gene            complement(1160..1564)
                     /locus_tag="PLA58_10235"
     CDS             complement(1160..1564)
                     /locus_tag="PLA58_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOR63212.1"
                     /translation="MPERKIRVMVAKPGLDGHDRGARILARCFRDAGFEVIYTGCHQT
                     PEQVAAAAIQEDVDLVGLSCLSGAHRILFPRVVELLREKNASDITVIGGGIIPEQDFP
                     MLYDAGIQAIFTPGASLDSIVEWVRTHIRGRN"
     gene            complement(1575..3257)
                     /locus_tag="PLA58_10240"
     CDS             complement(1575..3257)
                     /locus_tag="PLA58_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOR63213.1"
                     /translation="MYFSDETQKASRKLTEQWQANVQNTLKTNPDWKNRFSTVSDLDI
                     KRLYTPEDLQNTDFARDIGAPGEFPYLRGNQVTGYRGQPWTFRMFSGMGSAEDTNRRW
                     HLLLREGQTGLSTAFDFPTLMGYDSDSPKALGEVGKCGVAIDTLEDFLALIDNIPLDR
                     VTTSMTINPPATVLWAMYCAAAEIKGVPLNQIGGTIQNDMLKEFIAQKTFMCPPEPSV
                     RLISDTVEFGAKYVPRWNPISISGYHIREAGATAVQELAFTLRDGIEYVEDILRRKKM
                     SVDDFAPRLSFFFNAHIDLFEEICKLRAARRIWAKVMRDRFGAKNPRSLWMRFHVQTA
                     GCSLTAQQPYNNVVRTTVEALAAVLGGTQSLHTNSLDEVLCLPSEHAVEIALRTQQIL
                     AEETGVVNTIDPLAGSYFVESLTNEMEAKAWEYIEKIDAMGGMIAAIERGYPQMEIAD
                     AAYAFQRQIDAGEKIMVGVNKYATEAQHPVPLVEIDEKVGEEQIRRLKDLRRRRDSRA
                     VSQSLADIRAACKNGANVMPHCIAAVKALATEQEICDVYREVYGEYRDPGLY"
     gene            3312..>3505
                     /locus_tag="PLA58_10245"
     CDS             3312..>3505
                     /locus_tag="PLA58_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63214.1"
                     /translation="MQNKKTPSFQTVKKLTRIFRFCQEIFDAAPSNEEAPRGFPEGFF
                     ILNRSCFRLQILHPLPGLVI"
BASE COUNT          647 a         1012 c         1040 g          806 t
ORIGIN      
        1 actcgccttc ggctcaaaca gttgtcaaag gctgatcttt cgcttcgcca agatttatta
       61 aacaaaacgc tctcaatggc cgctcaaaga gatatttggg caacgggcat ttcggcccgt
      121 ctgaaagtgt gcgcaactct gaacgagggc ctttatgccc gcttcatggc gtttttcccg
      181 tattgaggaa acgacgggcg atggcttccg cttcggtata ggggtccgtt tcccggttct
      241 gaattttccc gacgattttt ttcagctcgc cgtccgtgat caggcgctcg atcaccggct
      301 ccaggatgca ggcgcgcagg gcgtcggtaa tttccatgac ggctttgcgt tccgtccgct
      361 cggccagttg tccgctgacg acaaggtgtg cgtggtgctt cgcgacggcg tccatcagct
      421 ccgccgtttt tttggcgaag ctttccggtt catacagatt gccgatgctg acgatgggcg
      481 gaatccagtc ccgggggccg tgcggtgagg cgttgagcag cgaggtcagc tccgttttca
      541 actgcttcgc gccgggacga tccgccttgt tgatgacgaa cacgtcggcg atttccatga
      601 cgcccgcctt catggtctgg atctcgtcgc ccatgccggg gaccagcacc acgacgatgc
      661 tgtgcgcgtg gtgaatgatg tcgagttcct gctggccgat gccgaccgtt tccaccagaa
      721 tgacatcctt gcccatggct tccatgacgt ggattgcgtc gcccgcggca tgtgccaatc
      781 cgcccagcgc gccccgcgtg gccagcgaac ggatgaacac gtcctcgtct tccgcgtggc
      841 gcagcatgcg gacgcggtcg cccagcagtg cgccgccgga gaaaggactg ctcggatcaa
      901 cggccagaac gcccacggtt ttttttcgtt tgcgcagctc gccgatcagc gcgtcggcca
      961 gtgtgctttt ccccgcgccg ggcgagccgg tgatgccgat aatgaaggat cggcccgtgt
     1021 gggggaaaag ctgccgcagc cgaaggcgcg cttcgggccg tttgtcttcc aggtcccgga
     1081 tcagcctgga ggccgagcgg atgtcgcctg cgcatatttt ttgggtcgtt tgggtcatgt
     1141 cgtgtttttg ccgcgccggt cagtttctgc cccgaatatg ggtgcggacc cattccacaa
     1201 tcgaatccag cgaagcgccg ggggtgaaga tcgcctggat gcccgcatcg tagagcatgg
     1261 ggaaatcctg ctcggggatg atgcctccgc cgatcacggt gatgtcggaa gcgttctttt
     1321 ctctgagcag ttccaccacg cgcggaaaca aaatgcggtg cgcgcccgac aggcagctta
     1381 aacccacaag gtccacgtct tcctgaatcg cggctgcggc aacctgctcc ggggtttgat
     1441 gacagcctgt gtaaatgact tcaaaaccgg cgtcgcgaaa acagcgggcc agaatgcgcg
     1501 cgccgcggtc gtggccgtcc agaccgggtt tggccaccat cactcgaatt tttctttccg
     1561 gcataatctt ctccttaata caaccccggg tccctgtact ccccgtaaac ctcgcggtaa
     1621 acatcgcaga tttcctgctc cgtggccagg gctttcaccg ccgcgatgca gtgcggcatg
     1681 acgtttgccc cgttcttgca cgcggcgcga atgtcggcaa gcgattgact cacggcgcgg
     1741 ctgtcgcggc gtctgcgcaa atctttcaac cgccgaatct gctcttcgcc cactttttca
     1801 tcgatttcca ccagcggcac cggatgctgc gcttcggtgg cgtatttgtt gacgcccacc
     1861 atgatttttt ctccggcgtc aatctgccgc tggaaggcat aggccgcatc cgcgatttcc
     1921 atttgcgggt agcctctttc gatggcggcg atcatgccgc ccatggcgtc gatcttttcg
     1981 atgtactccc aggcttttgc ctccatctca ttggtgaggg attccacaaa gtaggaaccg
     2041 gccagaggat cgattgtgtt gaccacgccc gtttcttcgg ccagaatctg ctgggtgcgc
     2101 agcgcgattt ccacggcgtg ctcggacggc aggcaaagca cttcatccag cgaattggtg
     2161 tgcagcgact gggttcctcc cagcaccgcg gccagcgcct cgacggtggt tcgaacgacg
     2221 ttgttgtaag gctgctgagc ggtgagcgag cagccggcgg tctggacgtg aaagcgcatc
     2281 cacagcgatc gcggattttt ggcgccgaag cggtcgcgca ttactttggc ccagatgcgg
     2341 cgcgcggcgc ggagtttgca gatttcctcg aaaaggtcga tatgcgcgtt gaaaaagaag
     2401 gaaaggcgcg gcgcgaaatc gtccacgctc attttcttgc ggcgaagaat atcttcgacg
     2461 tattcgatgc cgtctctgag cgtaaaggcc agctcctgca cggccgtggc gcccgcctcc
     2521 cggatatggt agccgctgat gctgatgggg ttccatcgcg gaacgtattt tgcgccgaat
     2581 tcgacggtgt cgctgatcag cctgacggaa ggctccggcg gacacatgaa cgtcttctgc
     2641 gcgatgaatt ccttgagcat gtcgttttgg atggtgccgc cgatttggtt cagggggacg
     2701 cccttgattt cggccgccgc gcagtacatg gcccacagca cggttgccgg cgggttgatc
     2761 gtcatggagg tggtgacgcg atccagcgga atattgtcga tcagcgccag aaaatcctca
     2821 agggtgtcga tggccacgcc gcacttgccg acctcgccca gggctttcgg cgagtcgctg
     2881 tcatagccca tgagcgtggg aaagtcgaag gcggtggaaa ggccggtctg tccttcccgc
     2941 aaaagcagat gccagcgccg gttggtgtcc tcggcgctgc ccatgccgga gaacatccgg
     3001 aacgtccagg gttgtccgcg gtatccggtg acctggttgc cgcgaagata gggaaattcc
     3061 cccggggcgc cgatgtcgcg ggcaaaatcc gtgttttgca ggtcttcggg cgtgtagagc
     3121 cttttgatgt caaggtcgga aacggttgaa aagcggtttt tccagtcggg attggttttt
     3181 aaagtatttt gaacattcgc ctgccattgt tcggtaagct tccgggacgc cttttgtgtt
     3241 tcatccgaga aatacaatgt aacctcctgc cgtcgttatg aacctttaaa aacttccagg
     3301 aaaaaagggt aatgcagaat aaaaaaacgc caagttttca aacggtgaaa aaactaaccc
     3361 gcattttccg cttctgtcaa gaaatattcg acgccgcgcc gtcaaacgaa gaagcccccc
     3421 ggggtttccc ggagggcttc tttatcttga acagatcatg ttttcggtta caaatccttc
     3481 atccgctgcc gggcctcgtg ataca
//
LOCUS       DAOSRM010000149         3428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_24781_length_3428_cov_72.472873, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000149 DAOSRM010000000
VERSION     DAOSRM010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24781_length_3428_cov_72.472873"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLA58_10250"
     CDS             <1..331
                     /locus_tag="PLA58_10250"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOR63215.1"
                     /translation="ALHITSCVIGFTMIKTFRCKDTASLFDDRLVLRFKAVERQARRK
                     LAYLHRARNLQDLRQPPGNQLEALKGERKGQFSIRIIDQWRICFTWESGDAYNVEIVD
                     YHLEKRK"
     gene            328..630
                     /locus_tag="PLA58_10255"
     CDS             328..630
                     /locus_tag="PLA58_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOR63216.1"
                     /translation="MKDLELLEPVTPGEILYEEFMKPLGISANQLARDVDVPPGRISE
                     IINGKRSITADTALRLGKYFGVSPEIWLNLQVDYDMRIARRTIWPKIESHVRVRAA"
     gene            780..2282
                     /locus_tag="PLA58_10260"
     CDS             780..2282
                     /locus_tag="PLA58_10260"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOR63217.1"
                     /translation="MKITVKKGSLADVKTEAFILVFCEGEKTLSGPAADIDRKSGGLI
                     ADIIKSGGFEAKPSQVTMIYSRTLAAKRIALVGLGKKNELTPEKIRRAFAKVMQELRG
                     SKVRKAAVSLDLSLLNVRKEKVVSAAVEGAVLGLYRYSPYKTVGREELQDVQEMTIVP
                     GPKDYALVRDEVQKTKIIADAVCFARDLVSAPANEMTPSILAEQARVVAKRKNVSCRV
                     LEKGQMKALGMNALLGVAAGSDQPPKLIVLEYTGGKKGEAPIALVGKGLTFDSGGISI
                     KPAEKMDEMKTDMAGGAAVLAVIRAAADLKLPVHLVGLVPATENLSGGSALKPGDVLK
                     SHSGRTIEVLNTDAEGRLILADALSYACRYKPSAIIDIATLTGACVVALGEEVAGMLG
                     TDDRMKKALREAADATGELIWELPLWDHYHELIKSDIADYKNSAGRLGGAITGAAFLS
                     KFVGEYPWVHLDIAGPAWTTKDRGYIPKGASGIPVRLLVEFLRNRVQEKK"
     gene            2583..>3428
                     /locus_tag="PLA58_10265"
     CDS             2583..>3428
                     /locus_tag="PLA58_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR63218.1"
                     /translation="MPPLLEIQNLKTVFTTERGVLRAVDGVSLSLSAGATLCVVGESG
                     CGKTMLALSIMRLIPPNGRIAEGRIYFNGQDLLKLSEEEMREKRGRDIAMIFQEPMTS
                     LNPVLRVGEQIAEAIRLHQHVSDREALDRSVKLLGEVGIPDPGRRVKDYPHQMSGGMR
                     QRVMIAMAMSCRPRLLLADEPTTALDVTIQAQILKLISDLKEKNNMAVIWITHDLGIV
                     AEAAQKVAVMYAGKIVEIADVERLFADPLHPYTRGLIESRPSGWAESDAEKGSYLKTI
                     PGTVPG"
BASE COUNT          817 a          829 c         1036 g          746 t
ORIGIN      
        1 tgctttacat attacgagct gcgtaatagg tttcaccatg attaaaactt ttcgttgtaa
       61 ggacacagcc tctcttttcg atgatcgttt agtccttcgc tttaaggctg ttgagcgaca
      121 ggcaagacga aaacttgcct atttgcatcg tgctcgtaac ctacaagacc ttagacagcc
      181 accgggcaat caattggaag cacttaaagg cgagcgaaag gggcaattca gcatccgtat
      241 cattgatcag tggcgcattt gttttacatg ggaaagcggt gacgcctata atgttgaaat
      301 cgttgattat catttggaga aaagaaaatg aaagatttgg aattattaga accggttact
      361 cccggtgaaa ttctttacga agaattcatg aaaccattgg gaataagcgc caatcagcta
      421 gcccgcgacg ttgatgtgcc gccgggacgg atcagcgaaa tcattaacgg caagcggtcg
      481 attaccgccg atacggctct tcgcctcggc aagtattttg gcgtgtcccc tgaaatatgg
      541 ctgaatctcc aggttgatta cgatatgcgg attgcacgca ggaccatttg gcccaaaata
      601 gagtctcatg tccgcgtccg ggcggcttaa caacatttct tgaaaacatg ctgttccagc
      661 ctattcctta ttatcgtgca tggtatgttg ttaattttag acctgtctgg ttgtgctcgt
      721 tttagtaaat gaaaatggcg ttcgaaatta catcagtttg ctttcatatg gaggtttaag
      781 tgaaaattac ggttaaaaag ggaagtcttg ccgatgtcaa aacggaggct tttattttgg
      841 ttttttgtga aggggagaag acgctgtccg gtcccgcggc ggatatcgac agaaaatccg
      901 gcgggctcat cgccgatatt ataaaaagcg gtggttttga ggcaaagcct tcgcaggtga
      961 cgatgatcta cagccggact ctcgcggcca aaagaatcgc gctggtggga ctgggcaaaa
     1021 aaaatgagct gacgccggaa aaaatccgca gggcttttgc gaaggtcatg caggagcttc
     1081 gcggatcaaa ggttcgtaaa gcggcagtgt cgctggattt gagccttttg aacgttcgaa
     1141 aggaaaaagt cgtgtctgcg gcggtggaag gggccgttct gggtctttac cgctattcgc
     1201 cttataaaac agtcggccgc gaagaattgc aggatgtgca ggagatgacc attgtgcccg
     1261 gtccgaagga ttatgccctc gtccgggatg aagttcaaaa aacaaaaatc attgcggatg
     1321 cggtttgttt tgcccgcgat ctggtttcgg cgccggccaa tgagatgacg ccgtcgattc
     1381 tggccgaaca ggcgcgggtg gtcgcgaaaa gaaaaaatgt ttcctgccgc gtgctggaaa
     1441 agggacagat gaaagcgctg ggcatgaacg cgctgctggg agtggccgcc ggcagcgatc
     1501 agccgcccaa gctgatcgtg ctcgaataca cggggggcaa aaaaggtgaa gccccgattg
     1561 ccctggtggg caaaggcctg acgttcgaca gcggcggcat atccatcaag cctgcggaaa
     1621 aaatggacga gatgaagacg gacatggcgg gcggcgcggc ggtgctggcc gttatccggg
     1681 cggcggcgga tctgaagctg cccgtccatc ttgtgggcct ggtgccggcc acggaaaacc
     1741 tctccggcgg cagcgcgctg aagccgggcg atgttttaaa atcccattcc ggtaggacga
     1801 tagaagtgct gaacacggac gcggaaggcc gcctgattct ggccgatgct ctctcctacg
     1861 cctgccggta caaaccctcg gcgatcatcg acatcgccac attgaccggc gcgtgcgtcg
     1921 tggccctcgg tgaagaggtg gccggaatgc tgggaacgga cgaccggatg aagaaggccc
     1981 tccgggaagc ggccgacgcg accggtgaac tgatctggga actgccgctg tgggatcatt
     2041 atcacgagct gatcaaaagc gacatcgccg attacaaaaa cagcgcgggc cggctgggag
     2101 gcgcgatcac gggagcggcc tttttgagca aatttgtcgg ggagtatccc tgggtgcatc
     2161 tggacatcgc ggggccggcc tggaccacga aagatcgagg ctacattccg aagggcgcgt
     2221 cgggcatccc cgtgcgcctg ctggtggaat ttttgcgaaa ccgggtgcag gaaaaaaagt
     2281 agtgcgaacc ttcaggttcg ctgtaaaggc gtctggattg atgaactcgt caatgtcgat
     2341 ttaggcccct ttgagtcata ccggcgaagg ccggtatcca gtaattaaat cagtcatgga
     2401 ccccggcctc cgccggggtg acggcagagt ttcgaaaaga ctggattccc gccagaagaa
     2461 tgcgggaatg accgatagga cagcaatgac ggcaatggag acttttttac gaaagcgtca
     2521 ggattagagc aaaccccttg gcggggctga agccacgcac tacattatct gtgagcagag
     2581 atatgccgcc gcttctggaa attcaaaatt taaaaaccgt atttacgact gaaaggggcg
     2641 tcctgcgggc cgttgacggg gtatccctgt cgctttccgc cggggcgacc ctgtgtgtcg
     2701 tgggggaatc cggctgcggg aaaaccatgc tggctctttc catcatgcgg ctgattccgc
     2761 ccaatggccg gattgccgaa ggccggattt acttcaacgg ccaggatctt cttaagcttt
     2821 cggaagaaga aatgcgcgaa aagcgcggcc gggacattgc catgattttt caggagccga
     2881 tgacgtccct caatcctgtt ttgcgcgtag gcgagcagat cgcggaggcc attcgcctgc
     2941 accagcatgt ttcggaccgg gaggcgctgg accgcagcgt gaaactgctg ggcgaggtcg
     3001 gcattcccga tccgggaaga agggtgaagg attatccgca tcagatgagc ggcggcatgc
     3061 gtcaacgggt catgattgcc atggccatgt cctgccgccc ccggttgctc ctggcggacg
     3121 aacccaccac ggcgctggac gtgactattc aggcgcagat tctgaagctg atttccgatc
     3181 tgaaggaaaa aaacaacatg gcggttattt ggatcacgca cgatctggga atcgttgccg
     3241 aggcggccca gaaggtggcg gtcatgtacg cgggcaagat cgtggaaatt gcggacgtgg
     3301 aaagattgtt tgccgatccg ctgcatccct acacgcgcgg gctgatcgaa tcacggccct
     3361 cgggatgggc agaaagcgat gcggaaaagg ggtcctattt aaaaaccatt ccgggaacgg
     3421 ttcccggc
//
LOCUS       DAOSRM010000150         3279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_26332_length_3279_cov_94.702854, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000150 DAOSRM010000000
VERSION     DAOSRM010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26332_length_3279_cov_94.702854"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /gene="tsaE"
                     /locus_tag="PLA58_10270"
     CDS             complement(<1..470)
                     /gene="tsaE"
                     /locus_tag="PLA58_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOR63219.1"
                     /translation="MNRVVVEMESESAGQTFAFGFSIGKRAEDGDVFALSGELGSGKT
                     CFTGGMARGLGVGEDYAITSPTFTLINEYPGRCRLYHCDVYRLNASSDLEDLGFDEYL
                     SGKGVLAIEWAEKISDALPQEAVSISFLYVDENRRKIRINGPRERVRDLLQDIS"
     gene            complement(467..877)
                     /gene="acpS"
                     /locus_tag="PLA58_10275"
     CDS             complement(467..877)
                     /gene="acpS"
                     /locus_tag="PLA58_10275"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOR63220.1"
                     /translation="MIYGIGIDLVENDRIGKIIRKWGPKFLSRVFTGEEIDYCGRHAQ
                     ASLHYGARFAVKESFLKAIGKGLGAGVKLAEIEVVNDPGGKPEIRLSGGARAFVERAG
                     IRKIHLSITHTKNYASAAVVLEKGWPDDWFADTR"
     gene            complement(874..2079)
                     /gene="folP"
                     /locus_tag="PLA58_10280"
     CDS             complement(874..2079)
                     /gene="folP"
                     /locus_tag="PLA58_10280"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOR63221.1"
                     /translation="MKIVTITQSSEAVALFQKIGVDPYGIRAMTPKTRHVNILLPARP
                     CKIANILKQEMLALGADAAVARGSVSCAVDATDVLLMGTHKQILALAGKIEKQPFGLD
                     GIACDLRRLLGHLEQKPLVLKTSRRELLLGDRTLIMGILNVTPDSFSDGNRYFDRRRA
                     IDRGLQMAEEGADIIDIGGESTRPGAKTVPLKEEIARVVPVVEQLAGKLSIPLSVDTT
                     KARVAEKALAAGAEIVNDISALSGDRKMASVIRKASAAVVLMHMRGTPADMQQGDLRY
                     DDLTGEIIAFLGDAVQKALAAGISRESLVIDPGIGFGKTYEDNCRILNRLDEFRALGL
                     PLLVGTSRKAFIGSVTGEAADQRTEGTAATVAAAVMRGCSIVRVHDVAAMKKVAAMTD
                     AIVRAGMAP"
     gene            complement(2105..>3279)
                     /gene="ftsH"
                     /locus_tag="PLA58_10285"
     CDS             complement(2105..>3279)
                     /gene="ftsH"
                     /locus_tag="PLA58_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOR63222.1"
                     /translation="SISGSDFVEMFVGVGASRVRDLFSQAKKNAPCIIFIDEIDAVGR
                     HRGAGLGGGHDEREQTLNQLLVEMDGFESNEGVILVSATNRPDVLDPALLRPGRFDRQ
                     VVVPLPDVKGREKIFEVHTRKIPVADDVDYSVLARGTPGASGADIENLVNEGVLNAAR
                     LNKEKVDMADFEFAKDKILMGTERKSMVISDLEKRNTAYHEAGHTLVARLIPGTDPIH
                     KVTIIPRGRALGLTQQLPVDEKHTYPSKYLENNIAVLLGGRAAEELILKDFTTGAGND
                     IERATNLARKMVCEWGMSKILGPLSYGRKEEQIFLGRDFATHKDYSEETAKKIDEEVI
                     SIVQRNYDKAVSLLDEHIEILHKMSRELLEKEVLNALEIDAIIGSALPAEHAAQTS"
BASE COUNT          636 a         1067 c          831 g          745 t
ORIGIN      
        1 ctagaaatgt cctgcagcaa atcccggacc ctttcccggg gtccgttgat ccttatcttt
       61 cgcctgtttt catccacgta cagaaaagaa atcgatacgg cctcctgcgg cagcgcgtcc
      121 gaaatcttct ccgcccattc gatggccaat acgcccttcc cagacaggta ctcgtcaaat
      181 cccaggtcct ccagatcgct cgacgcattc aaccggtaaa cgtcgcagtg gtaaagcctg
      241 caccgtcccg gatactcgtt gatcagcgtg aaggtcgggc tggtgatcgc gtagtcttcg
      301 cccacgccca gcccccgcgc cattccgccc gtgaagcatg ttttgcccga ccccagttct
      361 ccggaaaggg cgaaaacatc accgtcttcg gcccgcttcc cgatcgaaaa gccaaaggca
      421 aaggtttgcc cggcgctttc cgattccatt tcaacgacaa ctctgttcat cttgtatccg
      481 caaaccaatc atccggccag cctttttcca gcacgaccgc cgcggaagcg taatttttcg
      541 tgtgcgtaat gctcaggtgg attttacgga tcccggctcg ctccacaaag gcccgcgcgc
      601 cgccggacag acgaatctcc ggcttgcctc ccgggtcatt caccacctcg atctccgcca
      661 gtttcacgcc cgcgccaagg cccttgccga tcgccttgag aaacgactcc ttgaccgcaa
      721 accgcgctcc gtagtgaagc gaagcctgcg catgccgccc gcagtagtcg atttcttcgc
      781 cggtaaacac ccgggacaaa aatttaggcc cccatttccg gatgattttc ccgatgcggt
      841 cgttttccac caaatcgatg ccgattccgt aaatcatggc gccattcccg cgcgaacaat
      901 cgcgtccgtc atggccgcca cttttttcat ggccgcgaca tcatggacgc gaacgataga
      961 acagcccctc atcaccgccg cggcaaccgt cgccgcggtg ccttccgttc tttgatccgc
     1021 cgcctccccc gtaacactgc cgataaaagc tttgcgggag gttccgacca ggagcgggag
     1081 gcccagcgcc ctgaactcat ccagcctgtt gagaatccgg cagttgtcct cataggtttt
     1141 gccgaagccg attcccggat cgatcaccag actctcccgg ctgatgcccg ccgccagcgc
     1201 tttttggacg gcatctccga ggaaagcgat aatttcgccc gtcaggtcgt catagcgcag
     1261 atcgccttgc tgcatgtcgg ccggggttcc ccgcatgtgc atcagaacaa ccgccgccga
     1321 ggctttccgt atcaccgaag ccattttccg gtcgccggac agagcactga tatcattgac
     1381 aatttccgca cccgccgcga gcgctttttc agcaacccgg gcttttgtgg tatccacaga
     1441 caaggggatg gacagctttc cggccagttg ctccaccacg ggaaccaccc gggcgatttc
     1501 ttccttcagg ggaacggttt tcgctcccgg ccgggtggat tcgccgccga tatcgatgat
     1561 gtccgcgcct tcctccgcca tttgcaggcc ccggtctatg gccctccgac gatcaaaata
     1621 gcgattgccg tcggaaaacg aatccggggt gacgtttaaa atgcccatga tcagcgttct
     1681 gtctcccaga agcaattccc gtcgcgacgt tttcaggaca agtggttttt gttccagatg
     1741 gcccagcagc cggcgaaggt cgcaggcgat gccatccagt ccgaagggct gcttttcaat
     1801 ttttccggcc agagcgagga tctgcttgtg ggtccccatc agcaaaacgt ccgtggcgtc
     1861 caccgcgcag gacacgcttc cgcgcgcgac cgctgcgtcg gcgcccaaag cgagcatctc
     1921 ctgtttgaga atattggcga tcttgcaggg cctggcgggc aaaagaatat tgacgtgccg
     1981 tgttttgggc gtcatcgcgc gaatcccata aggatccacg ccgattttct gaaaaagggc
     2041 aaccgcttcg ctgctctgag taattgtaac gatcttcaaa gttgacggct tcgtaaaagc
     2101 cccctcagga agtctgcgcg gcatgctcgg ccggcagagc gctgccgata atcgcatcga
     2161 tctccagagc attgagaacc tctttttcca gaagctccct ggacattttg tgcagaattt
     2221 cgatatgctc gtcaagcagg gatacggctt tgtcatagtt gcgctggaca atcgaaatga
     2281 cttcctcgtc gatttttttt gccgtttctt cgctgtaatc cttgtgcgtc gcaaaatccc
     2341 gccccagaaa gatctgctct tccttccttc cgtagctgag cggccccagg atcttgctca
     2401 tgccccattc gcaaaccatc tttcgggcca gattggtcgc cctttcgatg tcgttgcccg
     2461 ctccggttgt aaaatccttc agaatcagct cttcggccgc gcgcccgccc aacagaacgg
     2521 cgatattgtt ttccagatac ttgcttgggt aggtgtgctt ttcatccacc ggcaactgct
     2581 gcgtcagtcc cagagcccgg ccgcgcggaa tgatcgtgac cttgtggatc ggatcggttc
     2641 cgggaatcag acgggccacc agcgtgtggc ccgcctcatg gtaggccgtg tttcgctttt
     2701 ccaggtcgct gatgaccatg ctctttcttt ccgtgcccat caggattttg tccttggcga
     2761 attcaaagtc cgccatatcg actttttcct tgttcagcct cgcggcgttc aacacgcctt
     2821 cattgaccag gttttcaatg tcggcccccg acgcgccggg cgttcccctg gccagaaccg
     2881 aataatccac gtcgtccgcg acgggaatct ttcgcgtgtg cacttcaaag atcttctccc
     2941 gccccttcac atccggcaaa ggcaccacca cctggcggtc aaagcgcccc ggtcgcaaaa
     3001 gcgccgggtc cagcacgtcg ggacggttgg tcgccgaaac cagaatcacg ccttcgttgg
     3061 actcgaagcc gtccatttcc accagcagtt ggttgagcgt ctgctcccgc tcgtcatgtc
     3121 cgccgcccag accggcgccc cgatggcgcc ctaccgcgtc gatttcatcg ataaaaatga
     3181 tgcagggggc gtttttcttt gcctggctga aaaggtcccg cacgcgggac gctccaacgc
     3241 cgacaaacat ttccacaaaa tcagagccgc tgatgctca
//
LOCUS       DAOSRM010000151         3106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_28311_length_3106_cov_133.980334, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000151 DAOSRM010000000
VERSION     DAOSRM010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28311_length_3106_cov_133.980334"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2221
                     /locus_tag="PLA58_10290"
     CDS             <1..2221
                     /locus_tag="PLA58_10290"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR63223.1"
                     /translation="ALADPDAFRKIVRDWAEVMKHDVTISLYTRFGTPFAINNSAGHG
                     TLPARNYRSGRPENFTTVSGNNIQKILFERGGKMHGCMPGCLVQCSIIYPDKNGKKIC
                     AAYEYETIALLGTNLGITDNDAIARLKFLCDDIGLDAIEAGSALGVAAEAGKMNWGDA
                     EGAENLLLEIEKETPLGFALGNGVVTTARFLNVERIPAFKGQALPAHDPRAVKGTGVT
                     YFSSPMGADHTAGLTYRQPKEKKDQIQTSLATQIKAAACDAFGYCLNAVPGGESVYPF
                     FAGLMNARYGLALTEEDILAAAKEALRDQLAFNEQAQFSRIDTTIPAFFREELIAPTS
                     SVFDVDEAEVRNLWKGLETFREKKKVWEIRIPPMPDILMGEGVAKSMGRKIRDMKVSK
                     IFLVTDPFMAKSGRAAEAADILKKSGIETEIFAEVEPDPPIELIERAGALYRETGCNG
                     ILGLGGGSSLDTAKTLGLRVTHPGDLREYEGIVGGGGKIKPIFPPLICLPTTSGTGSE
                     VNPCAVLTDKARDLKFILMSNHFIPKLAVIDPLFTRTMPPGLTIESGIDALSHCIEGY
                     VSLATPYHPYFESKALYGIKLIGRSLITACREPDNMRARTDMCMAALCGGLAFLKGLG
                     LGHALTHAIGAHYHLPHGRAAIFGLLGFVMANRETCRDAFMDMAYLINRTDDLEGALR
                     WLYKELQIDLRLKSYGISREALKEIAFYTSRDAVNMATDPTSPGQSKILELLSAMYE"
     gene            2406..>3106
                     /locus_tag="PLA58_10295"
     CDS             2406..>3106
                     /locus_tag="PLA58_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOR63224.1"
                     /translation="MLSGSLNRQIGLLINRKGEISMVILGDHRGIFIPGLDAFRAAST
                     RFKGLRLIHTHLDGEGLSPEDLADLSHLRLDMIGALQVQEDGSPGKLFWAHLIPENPQ
                     GAYWLIHDPQEPHRMNLNFLSFITALEDEFARRQKSRRIDAAEKAILVRVEKNPLEGA
                     EASLEELRQLASTCGVEVFDAQIQYRPQPDPKYLVGRGRLSDIDLRAAQIGANLLIFD
                     HELTPAQVRSIGDFTG"
BASE COUNT          672 a          988 c          888 g          558 t
ORIGIN      
        1 ggcgctggcc gatccggacg ccttccgcaa aatcgtccgc gactgggcgg aagtgatgaa
       61 gcacgacgtc accatcagtc tttacacgcg cttcggcacg ccgtttgcga tcaacaactc
      121 cgcggggcac ggaacccttc cggcccgcaa ttaccgctcc ggacgccccg aaaatttcac
      181 gacggtcagc ggcaacaaca tccagaagat tctgttcgag cgcggcggaa aaatgcacgg
      241 ctgcatgccc ggctgtctgg tgcagtgctc gatcatctat ccggacaaga acggcaaaaa
      301 gatctgcgcg gcgtatgaat acgaaacgat cgctcttctg ggaaccaacc tgggcatcac
      361 cgacaacgac gccatcgccc ggctgaagtt cctgtgcgac gacatcggcc tcgacgccat
      421 tgaggcgggc agcgccctgg gcgtcgcggc cgaagcgggc aaaatgaact ggggcgacgc
      481 cgaaggcgcg gaaaacctgc ttttggaaat cgaaaaagaa acgccgctcg gcttcgcgct
      541 gggcaacggc gtcgtgacga cggcccgttt tctcaatgtg gagcgcattc ccgccttcaa
      601 gggccaggcc ctgcccgccc acgatccccg cgcggtcaag ggaacgggcg tcacgtactt
      661 ttcctcgccc atgggcgcgg accacacggc cggcctgacc taccgccagc ccaaggaaaa
      721 gaaagaccag atccagacat cgcttgcgac gcagatcaag gcggcggcct gcgatgcgtt
      781 cggctactgc ctcaacgccg ttcccggcgg cgaatccgtt tatcctttct ttgccgggct
      841 catgaacgcg cgctacggcc ttgcgctgac cgaagaggac attcttgctg ccgccaagga
      901 agccctgcgc gaccagctgg ctttcaacga gcaggcgcag ttcagcagga tcgacacgac
      961 gattcccgcc tttttccgcg aggagctcat cgccccgacc agttccgtct tcgacgtcga
     1021 tgaagcggaa gtgcggaatt tatggaaagg cctggagacg ttccgggaaa agaagaaagt
     1081 ctgggaaatc cgcatcccgc cgatgcccga tattctgatg ggcgaaggcg tcgccaaatc
     1141 catgggccgg aaaatccgcg acatgaaggt gtcgaagatt ttcctggtca ccgatccgtt
     1201 catggccaaa agcggccgcg ccgccgaagc cgccgacatt ctgaagaaaa gcggcatcga
     1261 aacggaaatt ttcgccgagg tcgaaccgga cccgcccatt gaactcattg aacgcgcggg
     1321 cgctctgtac agggaaaccg gctgcaacgg cattttgggg ctgggcggcg gctcttcgct
     1381 ggacacggcc aaaacgctgg gcctgcgcgt cacgcatccg ggcgacctgc gcgaatacga
     1441 aggcatcgtc ggcggcggcg gcaaaatcaa accgatcttt ccgccgctca tctgcctgcc
     1501 gaccacctcc ggcaccggca gcgaggtcaa cccctgcgcc gttttgacgg acaaggcccg
     1561 cgatttgaag ttcatcctga tgagcaatca ttttattccc aaactggccg tgatcgatcc
     1621 tctcttcacc agaaccatgc cgccgggcct gaccattgaa tccggcatcg acgcgctttc
     1681 gcactgcatc gaaggctacg tttcgctcgc aaccccttac catccctact ttgaatccaa
     1741 ggcgctctac ggcatcaagc tgatcggccg gagcctcatc accgcctgcc gggagccgga
     1801 caatatgcgc gcgcgaaccg acatgtgcat ggcggccctg tgcggcggcc tggcgttcct
     1861 caaaggactg ggactgggcc acgccctcac ccacgccatc ggcgcgcatt atcacctgcc
     1921 gcacggacgg gcggccatct tcggcctgct gggcttcgtg atggccaaca gggaaacctg
     1981 ccgggacgcc tttatggaca tggcctacct catcaaccgg acggacgacc tggaaggagc
     2041 gctccgctgg ctttacaaag aactgcaaat cgacctgcgg ctcaaatcct acggcatttc
     2101 ccgggaagcc ctgaaggaaa tcgcctttta tacatcgcgc gacgcggtga acatggcgac
     2161 ggatccgacc tcgcccggac aatcgaaaat tctggaactg ctttcggcca tgtatgaatg
     2221 aacaaaaaca acgccgtttg cccgctgacg agcaaaacca ggcataagga aaacagacca
     2281 tcaataaaat attcggcaat acgtcaggac tcggcgcccg gcaaatcaaa tccctggaac
     2341 ggctttaccg gcgcggcatc ccgccggaaa gtctgatcag ccatgatctt gcccgcgaga
     2401 tttcattgct gtccggttcg ctcaaccggc agatcggcct gctgattaac cgcaaaggcg
     2461 aaatcagcat ggtgatcctg ggcgatcaca gaggcatctt catccccggc ctggatgctt
     2521 ttcgcgcggc gtccacccgc ttcaaggggt tgcgcctgat ccacacgcat ctggacggcg
     2581 aaggcctttc gccggaggat ctggcggacc tgtcccatct gcgcctggac atgatcggcg
     2641 ctcttcaggt acaggaagac ggttcgccgg gcaagctgtt ctgggcgcat ctgatcccgg
     2701 aaaatccgca gggcgcctac tggctgatcc atgatcccca ggagccgcac cgaatgaacc
     2761 tcaacttttt atccttcatc accgcgctgg aagacgagtt tgcccgccgg cagaaaagcc
     2821 ggcgaattga cgcggcggaa aaagccattc tggtccgtgt ggaaaaaaat cctctggagg
     2881 gcgctgaagc ctcgctggaa gaacttcggc aactggcctc cacctgcggg gtggaggtct
     2941 ttgacgcaca gattcaatac cgtccgcagc cggatccgaa atatctggtc ggccggggca
     3001 gattgtcgga catcgatctc cgggccgccc agatcggcgc gaacctgctg atctttgacc
     3061 atgaactgac gcccgcgcag gtgcgctcca tcggcgattt taccgg
//
LOCUS       DAOSRM010000152         2993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_29786_length_2993_cov_97.641253, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000152 DAOSRM010000000
VERSION     DAOSRM010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29786_length_2993_cov_97.641253"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PLA58_10300"
     CDS             <1..1215
                     /locus_tag="PLA58_10300"
                     /inference="COORDINATES: protein motif:HMM:NF039390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR63225.1"
                     /translation="DDTFRLIMKDKERLLDIQEPLRFIFSHSALREGWDSPNVFQICT
                     LNETHSELKKRQEIGRGMRLAVNQEGLRIQDKNVNRLTVIANEAYEDFARKLQAEIEE
                     DCHVSFQCRIKNKQKRETVKYRKGFELDAKFKDIWDKIKFQTTYKVDYDTPELIKAAA
                     KAVQEMPATKKAVIKSTKTAVEFDESGIIADVRASYNISIDGKFRIPDILFYIQERTE
                     LTRSTVLEVLLQSGRCGEVLINPQLFLDNAVAAINDVLNALMIDGIKYEKIGAKEYEM
                     RLFEDYDFHISDHTFEITRKDKTIYSHLLPLDSGVEYAFARDCEEREDIEFYFKLPNW
                     FKIKTPIGAYNPDWALIKKNEKTVYFVAETKSAGQELRTSEKQKVKCGRAHFREFPEV
                     SFRQATHVSDLD"
     gene            1222..2943
                     /locus_tag="PLA58_10305"
     CDS             1222..2943
                     /locus_tag="PLA58_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOR63226.1"
                     /translation="MAIKLDMDMAMQLAPRRARERGREYFEDGQVIDLAERKGLLIAK
                     VEGSRTYTVKMTSKADQNFHYSCTCPMGAEGEFCKHCVAVALAWIEGREVKQTKNAKE
                     IGLQDIETYLKTLDLSILVEILMEQAHRDETLRRKLFLKTARALSSGFDFAYWKRVIK
                     EAFDTQGFVNYHEASSFTDAASQTVDDIDELLEGRCSSEVISLAEYAIERAGTALESM
                     DDSNGEMGEVLNRLQNIHFKACRKVKPDPARLAKRLFHLELNSQFDEFYGAVKTYAPI
                     LGKAGLDAYRNLANQEWAKIPPGVNRWNAREKQSHTVYRLFHIMETLAELSGDVEQLV
                     AIKKRDLSSAYNYLTIAEIYKKAGNRDKALEWAEAGIKAFPERTDARLREFLADEYHR
                     RKRHEEALNLISLNFVDYLSLDRYQQLKQHADKTDAWPFWREKMMKYMQTHLTKQKKE
                     INRWSYDPGYSVLVEIFLWEKNPEAAWEAANAGGCSQSIWMKLAAIRGQDYPMDSVGI
                     YRKFVGPMIEQTNNQAYEDAIQLIKKIQAIMNRLGKENIFSGYVTELRTKYKAKRNFI
                     KLLDQIR"
BASE COUNT          860 a          674 c          777 g          682 t
ORIGIN      
        1 gacgatacat tcaggctcat tatgaaggac aaggaaaggc ttctggatat ccaagagcct
       61 ttgcgcttta ttttcagcca ttccgccctg cgcgaggggt gggacagtcc caatgttttc
      121 cagatttgca cgctcaacga gacgcattcg gaattgaaaa aacggcagga aatcgggcgg
      181 gggatgcgcc ttgccgtcaa tcaggaaggc ctgcgcatac aggacaaaaa cgtcaatcgt
      241 cttaccgtca ttgccaacga agcttacgaa gattttgcgc gcaagcttca agccgaaatc
      301 gaggaagact gccacgtgtc ctttcaatgt cggatcaaaa ataagcaaaa gcgggaaacc
      361 gtcaaatatc gcaaaggttt cgagctggat gcaaagttta aggacatctg ggacaagatc
      421 aaatttcaaa ccacttataa agtggactac gatacgccgg aattgatcaa ggcagccgcc
      481 aaagcggttc aggaaatgcc cgcaaccaaa aaagcggtca tcaaatcaac aaaaacagcc
      541 gtcgaatttg atgaatcggg tatcattgcc gatgtgcgcg cttcttacaa catatctatt
      601 gatggtaaat tccggattcc cgatatcctg ttttatattc aggaaagaac ggagttgacc
      661 cgctctacgg tgttggaggt tcttttacaa tcgggaaggt gcggcgaggt tctcatcaat
      721 ccccagttgt ttctcgataa tgccgtggcg gccatcaatg atgtattaaa tgcattaatg
      781 atcgacggta tcaagtatga aaaaatcggc gccaaggaat atgaaatgcg cttgtttgaa
      841 gactacgatt ttcatatctc cgatcataca ttcgaaatca cgagaaaaga caaaaccata
      901 tacagtcatc ttttgcctct cgattccggc gtcgaatacg cttttgccag ggattgcgaa
      961 gaacgtgagg atattgaatt ttatttcaag ctgcccaact ggtttaaaat taaaacgccg
     1021 atcggcgcct ataatccgga ctgggcgctg atcaagaaaa acgaaaaaac cgtttatttt
     1081 gtggcagaaa ccaaatccgc agggcaggaa ctgcggacgt ccgagaaaca gaaggtcaaa
     1141 tgcggacggg cacacttccg ggaatttcct gaagtgagtt tcaggcaagc gacccatgtc
     1201 agtgatctgg attaattgtt tatggcaata aagttggaca tggatatggc gatgcagctc
     1261 gcgccaagac gcgcacgcga acggggccgc gagtatttcg aggacgggca ggtgattgat
     1321 ctggccgaac gcaaggggtt gcttattgcc aaggttgaag ggagcaggac ttataccgtc
     1381 aagatgacct caaaggcgga tcaaaatttt cattattcct gcacctgccc gatgggggct
     1441 gaaggcgagt tttgtaaaca ttgcgtcgcc gtggcgctgg cctggatcga ggggcgggag
     1501 gttaaacaaa ccaaaaacgc caaagagatt ggccttcaag atattgaaac ctatttgaaa
     1561 acactggatt tatccatcct tgttgagata ctgatggagc aggcgcatcg ggatgaaacg
     1621 ctccggagaa agctttttct taaaacggcc agggcccttt cctccggatt cgactttgct
     1681 tactggaagc gggtgatcaa agaagccttt gatacacaag gctttgtcaa ttaccatgaa
     1741 gcgagcagtt ttaccgacgc cgcctcacaa acggttgacg acattgatga gttgctggaa
     1801 ggtcgctgtt cgtcggaggt catctcgctt gccgaatatg ccattgaacg ggcgggaact
     1861 gcattggagt cgatggatga ttccaacggc gaaatgggag aggttctgaa tcgcctgcag
     1921 aatatccatt tcaaagcttg ccggaaagtg aaaccggatc ccgcccggtt ggctaagcgt
     1981 ctttttcatc ttgaattgaa cagccagttc gatgaatttt atggcgcggt caaaacctat
     2041 gctcccattc tgggcaaagc gggactggac gcttatcgga acctggccaa tcaggagtgg
     2101 gcaaaaattc ccccgggcgt caaccggtgg aatgcccgtg agaaacaatc ccatacggtt
     2161 tatcgcctct ttcatatcat ggaaaccctg gcggagctct ccggtgatgt cgagcaattg
     2221 gttgcgatca aaaaacgaga tctgtcttcg gcatacaatt acctgaccat agccgaaata
     2281 tacaagaagg ccggcaacag ggataaagct ctggaatggg ctgaagcggg aatcaaggcg
     2341 tttcccgagc gcacagatgc ccgtttgcgg gaatttctgg cggatgaata ccaccggcgc
     2401 aaacgccacg aagaggcatt aaatctcata agtttgaatt ttgtggatta cctgtcgctg
     2461 gatcgctatc agcagttaaa gcagcacgcc gacaagaccg atgcatggcc tttctggcgt
     2521 gaaaaaatga tgaaatatat gcagacgcac ctgacgaaac agaaaaagga aatcaaccgc
     2581 tggagctatg atcccggtta ctccgtgctg gtggaaattt tcttgtggga aaaaaatccg
     2641 gaagcggcct gggaagcggc caacgccggg ggatgttcgc aatccatctg gatgaagctt
     2701 gcggctatcc gcgggcagga ttatccaatg gattccgtgg ggatttaccg gaaatttgtc
     2761 ggtccaatga ttgagcaaac aaacaatcaa gcctacgaag acgcaatcca gctgatcaaa
     2821 aaaattcaag cgatcatgaa ccggctcgga aaggaaaata tattttccgg ttacgtgacc
     2881 gaacttcgaa ccaagtacaa agccaagaga aacttcatca aactgctgga tcagattcgg
     2941 tagggaacgc tcgcgataac ctgagaactt tgaaaaatgt catttcgagg agc
//
LOCUS       DAOSRM010000153         2868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_31535_length_2868_cov_83.258087, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000153 DAOSRM010000000
VERSION     DAOSRM010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31535_length_2868_cov_83.258087"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="PLA58_10310"
     CDS             complement(<1..1238)
                     /locus_tag="PLA58_10310"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR63227.1"
                     /translation="MSFKKFQLNEKIMAGVAAAGYQEPTPIQDQSIAAIIQGRDIMGL
                     AQTGTGKTAAFVLPILQRLMSGKPRQVRALILAPTRELSEQTHEAIRELGQKTRLSSA
                     AIYGGVSIQSQITKLRSRVDIVSACPGRLLDHIGRGTIDLSGVEVLVLDEADHMFDMG
                     FLPDVRKIVKHLPAQRQTLLFSATMPHEIRSLADDLLCRPVHVEIGHIAPAMTVSHTF
                     YPVQMDQKTALLKDILEKTETTSVLVFTRTKSRAKNLAEQLNRAGHTVTSLHGNLTQQ
                     KRRQSLDGFRDGRYQVMVATDIAARGIDVTRISHVINYDMPATTDAYTHRIGRTGRAE
                     RTGDAFTFMTGQDGEQMRSLKKLLGDKLRYQNVEGLTIGPSACESMQTPAAAPKGFPS
                     RKNPFSSRPAATRRPVRRSF"
     gene            complement(1550..2761)
                     /locus_tag="PLA58_10315"
     CDS             complement(1550..2761)
                     /locus_tag="PLA58_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR63228.1"
                     /translation="MKKLLLIIMIVFSAFVVAIPAGAAANPGFDDKEIRIAQFGPQTG
                     PAAPWGAVARGGALLAEIVNAEGGIHGRKIKYFIRDDQYSPAVAQMVVKELVDKHGIF
                     AFTGGVSGAGGHAVKDYLAANKVLWVVPGTAALNTVADRDKNGMPSLYRYCSYPLFED
                     EASIVTKYAVEKLKYKKIGIVYQNDVFGKGGLTGLRQRLATYKMKPAEEAPVEPNEKD
                     LTSQMLRMKNSGAECVLMWVSPTLAVITLKNAQTVGYKPQWIAFDGLSDYQMMYKITG
                     GLFEGVITAAFVPPIDSNEPLVVKYREAAKKYTPEETWGTYVLAGIMFMEPLIEALKR
                     TGPNLSTEAVITELNKFKNWKGLGAPITWTPQVHQGTDSVQIQKCGPKGSYILLQDWT
                     RNELATWKKKK"
BASE COUNT          558 a          771 c          832 g          707 t
ORIGIN      
        1 ttaaacgaac gccggacggg gcgcctggtt gcggcaggtc gcgatgaaaa cggatttttc
       61 cggctgggaa atccctttgg cgcggctgcg ggtgtttgca tggactcaca ggcggatggt
      121 ccgatggtta acccttcgac gttctgatag cgcagcttgt cgcccagcag ctttttgagg
      181 gaccgcatct gttcgccgtc ctgtccggtc atgaaggtaa acgcatctcc ggttcgctcg
      241 gcgcgtccgg ttcgtccgat gcggtgcgtg taggcatcgg tggtcgcggg catgtcgtag
      301 ttgatgacgt gcgaaatccg ggtgacgtcg atgccgcggg cggcgatgtc cgtggcgacc
      361 atgacctggt agcgaccgtc ccgaaagccg tccagggact gcctgcgttt ctgctgcgtg
      421 aggttgccgt gcagggacgt cactgtatgt cctgctctgt tgagttgttc ggccaggttt
      481 ttggcgcgcg attttgtccg ggtaaaaacc agcacggaag ttgtctcggt tttttccaga
      541 atgtctttga gcagcgcggt tttctggtcc atctgcaccg ggtaaaaggt gtgggaaacg
      601 gtcatcgcgg gggcgatgtg tccgatctca acgtggacgg ggcggcaaag cagatcatcc
      661 gccaggctgc gaatctcgtg cggcatggtg gcggaaaaaa gcagggtctg gcgctgagcc
      721 ggcagatgtt tgacaatctt gcgcacgtcc ggcagaaatc ccatatcgaa catgtggtcg
      781 gcttcgtcca gaacgagcac ttcgacgcct gataaatcaa tggtgccgcg gccgatatgg
      841 tcgagcaatc tgcccgggca ggcggaaacg atatccacgc gcgaacgcag tttggtaatc
      901 tggctctgga tgctgacgcc tccatagatg gccgcgctgg aaagccgggt cttttgtccc
      961 agctcgcgaa tcgcctcgtg ggtctgttcg gacaattccc gtgtgggcgc gaggatcagg
     1021 gcgcgaacct gacgcggttt ccccgacatc aggcgctgta aaatcggcag cacaaaggcc
     1081 gcggtcttgc cggttccggt ttgggccagt cccatgatgt cccggccttg gatgatcgcc
     1141 gcgatggact gatcctggat gggggtcggc tcctgatagc cggcggcggc cactcccgcc
     1201 ataatttttt cattcaactg aaacttctta aaactcattt tatatcctta attttatgta
     1261 ccagcgtctg ttcaaattcc atccgatgca aaaccgttat aaggctggta aaagccggcg
     1321 cccgcaaacg ctgatgatga cgtctgaagg acaggcctca aacacggcgc ggatcctgtt
     1381 gtgattagcg ggattatcct taacaaattt ctgatttaga aaggtgggaa ggtcaatggg
     1441 cttcgctatt cattgcggca ttaatacagg caatccgtat aaatagcaag gaatatcttt
     1501 tgaaataaac agacaggcga ggcagccggg ccccgcctgt cttgtgtttc tattttttct
     1561 ttttccaggt ggccagttcg ttcctggtcc agtcctgcag cagaatgtaa ctgcctttgg
     1621 gaccgcattt ctggatctgg acggagtcgg tgccctgatg cacctgcggc gtccaggtga
     1681 tcggcgctcc cagtcccttc cagtttttga atttattgag ctcggtgatg acggcttccg
     1741 tgctcagatt cgggccggtt cgcttgaggg cctcgatcag cggctccatg aacatgatgc
     1801 cggccagaac gtatgtgccc caggtttcct ccggcgtgta tttcttggcg gcttcccggt
     1861 attttacaac gagcggttca ttggagtcga tgggcggcac gaacgcggcg gtgatcacac
     1921 cttcaaagag tcctccggtg attttgtaca tcatctgata gtcggaaagg ccgtcaaacg
     1981 cgatccattg gggcttgtag ccgaccgtct gggcgttctt gagcgtgatg accgccaatg
     2041 tggggctgac ccacatgagg acgcattccg cgccggaatt tttcatccgg agcatctgcg
     2101 aggtcaggtc tttttcattc ggctccaccg gggcttcttc ggcgggcttc atcttgtaag
     2161 ttgccaggcg ctgccgcaaa ccggtcaggc cgccttttcc aaacacatcg ttctggtaaa
     2221 cgatgccgat cttcttgtat ttgagttttt ccacggcata cttggtgaca atgctcgcct
     2281 catcctcaaa cagggggtag gagcagtaac ggtaaaggct cggcatgccg tttttgtccc
     2341 tgtcggccac cgtgttgagc gcggcggtcc ccggaacgac ccagagcact ttattggccg
     2401 ccagataatc tttcaccgcg tgccctccgg cgccggaaac gccgccggta aaggcaaaga
     2461 tgccgtgttt gtccaccagc tccttgacca ccatctgggc caccgccgga ctgtactgat
     2521 catcccgaat aaaatatttg attttgcggc cgtggatgcc gccttccgcg ttcacaattt
     2581 cagccagcag agcgccgccg cgcgcaaccg cgccccaggg tgcggcagga cctgtctggg
     2641 ggccgaactg ggcaatgcga atttctttgt catcaaaacc gggattggct gcagcaccag
     2701 ccgggattgc cactacaaag gctgagaaca cgatcattat gataagcaag agcttcttca
     2761 tagatttctc cttttctccg ataggctgat ttgtggatca agttttctga aaactcattt
     2821 tttgacaagt tcgtaaaaag tcattttcgc acccttttcg tcataccg
//
LOCUS       DAOSRM010000154         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_38559_length_2474_cov_98.751964, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000154 DAOSRM010000000
VERSION     DAOSRM010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38559_length_2474_cov_98.751964"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="PLA58_10320"
     CDS             <1..1536
                     /locus_tag="PLA58_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR63229.1"
                     /translation="SMSHEIRTPMNAILGMADLLSETPLTQEQMKFVQVFKEAGENLL
                     NIINDILDISKVEAGQVHLEHIAFDLGEMIERIGDIMSTRAHKKGLELTCHISPEVPM
                     NLMGDPIRLRQVLVNLLGNAMKFTEAGEVLLDVSMAAQTTGDVDRNTAELIFSVIDTG
                     IGIPADKAQDIFERFTQADASTTRKYGGTGLGLTISKSLVELMGGKIAVDSEEGKGSV
                     FFFTIPFALQTEPLVADSCLEDLMGRRGLIIDDNATNRLIVREMLTGWGATSTSVDNG
                     PSGITALQEAQTSHQPYDFVVLDYQMSAMDGLTTASEIRKDPGLSSTAIIMLSSSYPQ
                     AEIMEARKLGIDHFLYKPVKRNDLRDAICSALGKLRMSGPKPAVVTGAPAAQKALKIL
                     LVEDNEDNRLLVSTFLKNTPHHLVMAENGAVGVEAFKAGDYDMVFMDVQMPVMDGYTA
                     TREIRQWETAEGRPAVPIIALTAHALKEDEQKSFDAGCNGHLTKPIKKTKLLAALAEY
                     TAD"
     gene            complement(1625..>2474)
                     /locus_tag="PLA58_10325"
     CDS             complement(1625..>2474)
                     /locus_tag="PLA58_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOR63230.1"
                     /translation="FCRIIAIYSKMLHLSIEHVGESPVEDSVNTATVRLAADVGFDEV
                     LRTARAAGITSPLLPVPSMALGSFEVSPLELAYAYATLASGGTRFERFALFSVTTPDG
                     DRLWSGKMKRRQVFDPRATYLTGYALEGVLERGTAREAKSLHLVFPVSGKTGTTNGNR
                     DSWFVGYTPDVVCAVWVGYDSGADTGLTGAAGALRIFARFMRAFYSVSGPPAAAVPDG
                     IEMARIDPESGYLATSRCPRTFPEAYLRGTAPGETCPDHPVHPVMDALRNKMREAGGF
                     LRELFR"
BASE COUNT          588 a          672 c          721 g          493 t
ORIGIN      
        1 agtatgagcc atgagatccg gacgcccatg aacgccatcc tcggcatggc ggatctcctg
       61 agtgaaacgc ctttgaccca ggaacagatg aaatttgtgc aggtgtttaa agaagcggga
      121 gagaatcttc tgaacatcat caacgatatc ctcgatattt ccaaggtgga agccgggcag
      181 gtccatctgg agcatattgc gtttgatctc ggggaaatga ttgagcggat cggcgacatc
      241 atgtccacca gggcccacaa gaaaggtctg gaattaacct gccatatttc tccggaggtc
      301 ccgatgaacc ttatgggcga tcccatacgg ctgcgccagg tactggtgaa ccttttgggc
      361 aatgccatga aattcaccga agcgggagaa gtcctgctgg atgtgagcat ggcggcgcaa
      421 accaccgggg acgttgatcg aaatacggcg gaattgatct tttccgtcat tgatacgggc
      481 atcggaattc ccgccgacaa ggcacaggac atctttgagc ggtttacaca ggccgacgct
      541 tcaaccacgc gtaaatacgg cggaacggga cttgggctga cgatttccaa atcgctcgtt
      601 gaactcatgg gggggaaaat tgcagtggac agtgaagagg gaaaaggaag cgttttcttt
      661 tttacgattc ctttcgcgct tcagacagag cccttggttg cagactcgtg tctggaggat
      721 ttaatgggcc gtcgaggtct catcatcgac gataatgcca cgaatcgctt gattgtccgg
      781 gaaatgctga caggatgggg agccaccagc acctccgtgg acaacggccc gtccggcatt
      841 acggcgttgc aggaagcgca aacatcccat cagccctatg attttgttgt attggattat
      901 cagatgtccg ccatggatgg actgacgaca gcctcggaaa ttcgtaagga ccccggcctg
      961 tcgtccacgg cgattattat gctttcatct tcctatcccc aggcggaaat catggaggca
     1021 agaaaactcg gcatcgatca ttttctctat aaacccgtca agcggaacga tttgagggat
     1081 gcgatctgtt cggcgctggg gaaactgcgg atgtcggggc caaaaccggc ggttgttacc
     1141 ggtgcccccg cggcacaaaa agccctgaaa attcttctgg tggaggacaa cgaggacaac
     1201 cgccttctgg tctcaacctt tctgaaaaat acgccgcatc atctggtgat ggcggaaaac
     1261 ggcgctgtcg gcgtcgaggc cttcaaggcg ggagactacg acatggtctt catggatgtg
     1321 cagatgcccg ttatggacgg ctatacggcc acccgtgaga tacgccaatg ggaaacagcc
     1381 gaagggcggc ccgccgttcc catcatcgcc ttgaccgctc acgccttgaa agaggacgaa
     1441 caaaaaagtt ttgacgccgg ctgcaacgga cacctgacca agccgatcaa aaagacaaaa
     1501 ctgctggcag ccctggccga gtataccgct gattagggga tgttgttcgt gaagccgcag
     1561 caaatccgca tccttcatgc aagccgggat cagggctgct ggttgaaaaa gtctgcaaaa
     1621 aactttatcg gaacagttcg cgcagaaaac cgccggcttc gcgcatttta tttcggagtg
     1681 cgtccatgac cgggtggacc ggatggtcag ggcaggtttc cccgggagcc gttccccgaa
     1741 ggtaagcttc cggaaaggtc cgggggcacc gtgatgtggc gaggtatccg gattccggat
     1801 caatccgtgc catttcgatc ccgtccggaa cggcggcggc cggcggtccg gagaccgaat
     1861 agaaggcacg catgaaacgc gcgaaaatcc gcagcgctcc ggccgcgccc gtcagaccgg
     1921 tatccgcccc ggagtcatac cccacccata cggcgcagac gacgtcgggc gtatagccca
     1981 cgaaccagga gtcccggttg ccgttggttg ttccggtttt gcccgacacg ggaaaaacca
     2041 aatgcaggga ctttgcctcc cgggccgtgc cgcgctccag gaccccttcc agagcgtagc
     2101 cggtcagata cgttgcccgc ggatcaaaga cttgcctgcg tttcattttt ccggaccaga
     2161 gccggtctcc gtccggcgtc gtcacggaaa aaagggcaaa ccgttcaaag cgcgttccgc
     2221 cggaagcaag ggtcgcgtag gcataggcaa gttcgagggg cgatacttcg aagctgccca
     2281 gcgccatgga gggaacgggc agaaggggac tggtgatgcc cgccgcccgg gcggttctca
     2341 aaacctcatc gaaccccacg tcggcggcca gccggacggt ggccgtgttg acggaatctt
     2401 cgacaggact ttcgccaaca tgctcaatcg acagatgtaa catcttggaa taaatagcga
     2461 taatccgaca aaaa
//
LOCUS       DAOSRM010000155         2286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate AMR_MDS_2267
            NODE_42971_length_2286_cov_105.374272, whole genome shotgun
            sequence.
ACCESSION   DAOSRM010000155 DAOSRM010000000
VERSION     DAOSRM010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864334
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (wastewater metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:58:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,026
            CDSs (with protein)               :: 2,026
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42971_length_2286_cov_105.374272"
                     /isolate="AMR_MDS_2267"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="PLA58_10330"
     CDS             <1..1440
                     /locus_tag="PLA58_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR63231.1"
                     /translation="LRQQKKRIALVHPRGSNWFPGRRDITDIANRMVPQGMLSIAAWL
                     TRQGHDVFVYDCLGPGAPVDIDRQARDILAFDPKVVGFSATTSSFPDAADLAKKIKDS
                     RPDIVTVCGGVHVSALREKLLADYPAFDYLAAGEGELTLGELAAGVDPSEITGLIRRE
                     NGVAVANPPRKHIPDLDSLSFPAYEKLCGFPRDYHLPLFSYIRIPGATMITSRGCMYQ
                     CSYCDRSVFQKGFRYNSAAYIYEHMKYLRDRFGVRHVNIYDDLFTANRKRIVELCEKL
                     ARFPLGVNFNCAVRVGYTDDELLGMLKAAGCLMVSLGIESADPDMLARHKSGVSLDDV
                     RDTVRRIQAAKLRAKGLFMMGLPGETEASIRRTSDFIISLGLDDMNMAKFTPFPGAPL
                     WSTIHGEGTFKEDWRLMNCLNFVFVPRGIESKEKLDHLYNEYVKRFYSDPAWRRRFRG
                     RIWEHRHSLLYLLRHLPSFWSAKNQFEPE"
     gene            1580..1972
                     /locus_tag="PLA58_10335"
     CDS             1580..1972
                     /locus_tag="PLA58_10335"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOR63232.1"
                     /translation="MEKDDVVKYWIESSDSDFQVMESLFENGHYLWSLFLGHLVIEKL
                     LKAYHVKKVDTDYPRIHNLPEIARRASLALSDEQKFVMAELTTFNLRARYPDYKNRFQ
                     KKADRQYTETHLNKIREMRKWLQEKIKE"
     gene            1945..2268
                     /locus_tag="PLA58_10340"
     CDS             1945..2268
                     /locus_tag="PLA58_10340"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOR63233.1"
                     /translation="MVTGKDKRVIYEKIRDYVKTLEKNKVPVWRLYLYGSYAKNTFHP
                     DSDIDLAVFWDRDDIDGLDEDIQLRMLSWEVDLMIDPHSFARTDFDETDPFVREIIAT
                     GERII"
BASE COUNT          556 a          590 c          604 g          536 t
ORIGIN      
        1 ttacgtcaac aaaaaaaacg cattgccctg gttcatcccc gcgggtccaa ctggtttccg
       61 ggcagacgcg acatcaccga catcgccaac cggatggtgc cgcagggaat gctctccatc
      121 gcggcctggc tgacgcgcca agggcatgac gttttcgttt atgactgcct cggtcccggc
      181 gctccggtgg atatcgaccg gcaggcccgg gatattcttg cgttcgaccc taaagtcgta
      241 ggcttttccg ccaccacgtc atcctttccc gacgcggctg acctcgcgaa aaagatcaag
      301 gacagccggc ccgatattgt taccgtctgc ggtggcgtgc atgtctcagc cttaagggaa
      361 aagcttctgg cggactatcc cgccttcgat taccttgcgg cgggtgaggg cgaactgacc
      421 ttgggtgaac tggctgcggg cgttgatccc tcagaaatca cgggattgat ccgcagagaa
      481 aacggcgtgg ccgttgccaa ccctccaaga aaacatattc ccgacctcga cagcctgtct
      541 tttccggctt acgaaaagct gtgcggattt ccccgcgact accacctgcc gcttttcagc
      601 tacatccgga ttcccggcgc gacgatgatc acctcccgcg gctgcatgta tcaatgctcc
      661 tactgcgatc gctccgtttt tcagaagggc ttccgctaca actcggcggc ctacatctat
      721 gaacacatga aatatctgcg cgaccggttc ggcgtgcgcc acgtcaacat ctacgatgac
      781 ctgtttaccg ccaaccggaa acgtatcgtg gagctgtgcg aaaaactcgc tcgctttccg
      841 ctgggcgtga acttcaactg cgccgtgcgc gtgggctaca cggacgatga actgctcggg
      901 atgctcaagg cggcgggctg cctgatggtg tctctgggga tcgaatcggc cgatccggac
      961 atgcttgccc gccacaaatc cggcgtgtcg ctggacgatg tgcgcgacac cgtgcggcgc
     1021 attcaggcgg cgaaactccg cgccaaaggc ctgttcatga tggggctgcc gggggagacc
     1081 gaagcgtcga tccggcggac ctccgatttc attatctcgc tggggctcga cgacatgaat
     1141 atggccaagt tcacgccgtt tcccggcgcg ccgctgtggt caacgattca tggggagggg
     1201 actttcaaag aagactggcg gctgatgaac tgcctgaact tcgtttttgt tccgcgcggg
     1261 attgaatcaa aagaaaaact cgatcatctc tacaacgagt acgtcaagcg cttttactcc
     1321 gatccggcct ggcgcaggcg gttccggggg cgcatctggg agcaccgcca cagtctgctg
     1381 tatctgctga ggcaccttcc ctccttctgg tcggccaaaa accagtttga gccggagtaa
     1441 ggccggcaga tttcacaagg attatagatt ttaccgttat tggattcggt gttcagcatg
     1501 gtgtttgtcg ggatagctga caaacaccat cacttaataa tttgttgata atcaaggatt
     1561 catcatgtaa attacggtca tggaaaagga tgatgttgta aaatactgga ttgaatcatc
     1621 ggattctgat tttcaggtca tggagagtct gtttgaaaac gggcattact tatggtcatt
     1681 atttttgggg cacttggtta ttgaaaaatt gctgaaggca taccatgtaa aaaaagttga
     1741 caccgattat cccagaatac ataatttgcc ggaaattgcc cgcagggcgt ctcttgcact
     1801 atccgacgag caaaagtttg ttatggcgga gttgactaca tttaatctca gggcaagata
     1861 ccctgattat aagaaccgat ttcaaaaaaa agccgatcgc caatatacgg agacgcacct
     1921 caacaaaata cgggaaatga gaaaatggtt acaggaaaag ataaaagagt aatttacgaa
     1981 aagatcaggg actacgtaaa gacactggaa aaaaacaagg tgccggtctg gcgtttatat
     2041 ctttacggct cctatgcaaa aaatacattt catcccgaca gcgatattga tctggccgtg
     2101 ttctgggatc gcgacgacat tgacggcctg gacgaagata ttcaattaag aatgttgagc
     2161 tgggaagtcg atctgatgat tgatccgcat tcttttgccc gcaccgattt tgatgaaacc
     2221 gatccgttcg tccgggaaat catcgcaaca ggggaacgca ttatctaaag aagtttgcaa
     2281 tagaaa
//
